SMED30024013

Overview
NameSMED30024013
Smed IDSMED30024013
Length (bp)1790
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30024013 (SMED30024013) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30024013 (SMED30024013) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30024013 (SMED30024013) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30024013 (SMED30024013) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30024013

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 5

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
Smed sexual biotypeSMED30024013h1SMcG0001721 Contig31507newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30024013h1SMcG0001721 Contig31507uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
nervous systemSMED30024013h1SMcG0001721 dd_Smed_v4_16281_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30024013h1SMcG0001721 dd_Smed_v4_16281_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30024013h1SMcG0001721 dd_Smed_v4_16281_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30024013 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.006711:27701..31160 +8682
Homology
BLAST of SMED30024013 vs. Ensembl Human
Match: MECOM (MDS1 and EVI1 complex locus [Source:HGNC Symbol;Acc:HGNC:3498])

HSP 1 Score: 132.109 bits (331), Expect = 2.003e-31
Identity = 56/80 (70.00%), Postives = 67/80 (83.75%), Query Frame = 3
Query: 1512 RYHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIRQ 1751
            RY C  C K FPR+ANL RHLRTHTGEQPY+C +CD+ FSISSN+QRHVRNIHNKE+P+KC  CDR F Q T+LDRH+++
Sbjct:  723 RYTCRYCGKIFPRSANLTRHLRTHTGEQPYRCKYCDRSFSISSNLQRHVRNIHNKEKPFKCHLCDRCFGQQTNLDRHLKK 802          

HSP 2 Score: 118.627 bits (296), Expect = 4.095e-27
Identity = 75/223 (33.63%), Postives = 111/223 (49.78%), Query Frame = 3
Query:  276 QFQCPDCGANFSSRGPLKRHQNALCKNPNGLLIKTRQTIFKHNSVIQRKQVLGTNQNCTT-----TNLSGGSEHRKTSSDYKQ--------SVSFPISKLSSPISNGSNLNLFCDVCNKPCRSQVSLIRH-RKQHQNPNSHYSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYTQYSNLCRHKRMSRDCQRKAKCTECGMEFTNGKTYDTHKQHCLG 902
            Q++C DC   F S+  L  HQ   C  P+       +   +        Q + T Q C        +L    +H  + ++ ++        + ++  + +   +S+ S  +  C+ C K      +L RH R QH    +H +C  CGKTF  SSGLKQH HIHSS KP+ C  C KSYTQ+SNLCRHKRM  DC+ + KC +CG  F+   + + H++ C G
Sbjct:   20 QYRCEDCDQLFESKAELADHQKFPCSTPHSAFSMVEEDFQQKLESENDLQEIHTIQECKECDQVFPDLQSLEKHMLSHTEEREYKCDQCPKAFNWKSNLIRHQMSHDSGKHYECENCAKVFTDPSNLQRHIRSQHVGARAH-ACPECGKTFATSSGLKQHKHIHSSVKPFICEVCHKSYTQFSNLCRHKRMHADCRTQIKCKDCGQMFSTTSSLNKHRRFCEG 241          

HSP 3 Score: 60.8474 bits (146), Expect = 5.585e-9
Identity = 29/76 (38.16%), Postives = 45/76 (59.21%), Query Frame = 3
Query: 1521 CHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIR 1748
            C  C + FP   +L +H+ +HT E+ YKC  C K F+  SN+ RH  + H+  + Y+C  C ++F   ++L RHIR
Sbjct:   77 CKECDQVFPDLQSLEKHMLSHTEEREYKCDQCPKAFNWKSNLIRHQMS-HDSGKHYECENCAKVFTDPSNLQRHIR 151          

HSP 4 Score: 57.7658 bits (138), Expect = 5.020e-8
Identity = 28/77 (36.36%), Postives = 41/77 (53.25%), Query Frame = 3
Query: 1512 RYHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRH 1742
             Y C  C K F   +NL RH  +H   + Y+C  C K F+  SN+QRH+R+ H   R + C +C + F   + L +H
Sbjct:  102 EYKCDQCPKAFNWKSNLIRHQMSHDSGKHYECENCAKVFTDPSNLQRHIRSQHVGARAHACPECGKTFATSSGLKQH 178          

HSP 5 Score: 55.8398 bits (133), Expect = 2.230e-7
Identity = 24/56 (42.86%), Postives = 36/56 (64.29%), Query Frame = 3
Query: 1587 GEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIRQL 1754
            G++ Y C +C K F  S+N+ RH+R  H  E+PY+C  CDR F   ++L RH+R +
Sbjct:  720 GKERYTCRYCGKIFPRSANLTRHLR-THTGEQPYRCKYCDRSFSISSNLQRHVRNI 774          

HSP 6 Score: 53.5286 bits (127), Expect = 1.051e-6
Identity = 30/83 (36.14%), Postives = 43/83 (51.81%), Query Frame = 3
Query:  657 YSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYTQYSNLCRHKRMSRDCQRKAKCTECGMEFTNGKTYDTH-KQHCLG 902
            Y+C  CGK F  S+ L +H+  H+  +PY C  C +S++  SNL RH R   + ++  KC  C   F      D H K+H  G
Sbjct:  724 YTCRYCGKIFPRSANLTRHLRTHTGEQPYRCKYCDRSFSISSNLQRHVRNIHNKEKPFKCHLCDRCFGQQTNLDRHLKKHENG 806          
BLAST of SMED30024013 vs. Ensembl Human
Match: MECOM (MDS1 and EVI1 complex locus [Source:HGNC Symbol;Acc:HGNC:3498])

HSP 1 Score: 131.724 bits (330), Expect = 2.224e-31
Identity = 56/80 (70.00%), Postives = 67/80 (83.75%), Query Frame = 3
Query: 1512 RYHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIRQ 1751
            RY C  C K FPR+ANL RHLRTHTGEQPY+C +CD+ FSISSN+QRHVRNIHNKE+P+KC  CDR F Q T+LDRH+++
Sbjct:  723 RYTCRYCGKIFPRSANLTRHLRTHTGEQPYRCKYCDRSFSISSNLQRHVRNIHNKEKPFKCHLCDRCFGQQTNLDRHLKK 802          

HSP 2 Score: 118.242 bits (295), Expect = 5.201e-27
Identity = 75/223 (33.63%), Postives = 111/223 (49.78%), Query Frame = 3
Query:  276 QFQCPDCGANFSSRGPLKRHQNALCKNPNGLLIKTRQTIFKHNSVIQRKQVLGTNQNCTT-----TNLSGGSEHRKTSSDYKQ--------SVSFPISKLSSPISNGSNLNLFCDVCNKPCRSQVSLIRH-RKQHQNPNSHYSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYTQYSNLCRHKRMSRDCQRKAKCTECGMEFTNGKTYDTHKQHCLG 902
            Q++C DC   F S+  L  HQ   C  P+       +   +        Q + T Q C        +L    +H  + ++ ++        + ++  + +   +S+ S  +  C+ C K      +L RH R QH    +H +C  CGKTF  SSGLKQH HIHSS KP+ C  C KSYTQ+SNLCRHKRM  DC+ + KC +CG  F+   + + H++ C G
Sbjct:   20 QYRCEDCDQLFESKAELADHQKFPCSTPHSAFSMVEEDFQQKLESENDLQEIHTIQECKECDQVFPDLQSLEKHMLSHTEEREYKCDQCPKAFNWKSNLIRHQMSHDSGKHYECENCAKVFTDPSNLQRHIRSQHVGARAH-ACPECGKTFATSSGLKQHKHIHSSVKPFICEVCHKSYTQFSNLCRHKRMHADCRTQIKCKDCGQMFSTTSSLNKHRRFCEG 241          

HSP 3 Score: 60.8474 bits (146), Expect = 6.017e-9
Identity = 29/76 (38.16%), Postives = 45/76 (59.21%), Query Frame = 3
Query: 1521 CHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIR 1748
            C  C + FP   +L +H+ +HT E+ YKC  C K F+  SN+ RH  + H+  + Y+C  C ++F   ++L RHIR
Sbjct:   77 CKECDQVFPDLQSLEKHMLSHTEEREYKCDQCPKAFNWKSNLIRHQMS-HDSGKHYECENCAKVFTDPSNLQRHIR 151          

HSP 4 Score: 57.7658 bits (138), Expect = 5.601e-8
Identity = 28/77 (36.36%), Postives = 41/77 (53.25%), Query Frame = 3
Query: 1512 RYHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRH 1742
             Y C  C K F   +NL RH  +H   + Y+C  C K F+  SN+QRH+R+ H   R + C +C + F   + L +H
Sbjct:  102 EYKCDQCPKAFNWKSNLIRHQMSHDSGKHYECENCAKVFTDPSNLQRHIRSQHVGARAHACPECGKTFATSSGLKQH 178          

HSP 5 Score: 55.4546 bits (132), Expect = 2.447e-7
Identity = 24/56 (42.86%), Postives = 36/56 (64.29%), Query Frame = 3
Query: 1587 GEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIRQL 1754
            G++ Y C +C K F  S+N+ RH+R  H  E+PY+C  CDR F   ++L RH+R +
Sbjct:  720 GKERYTCRYCGKIFPRSANLTRHLR-THTGEQPYRCKYCDRSFSISSNLQRHVRNI 774          

HSP 6 Score: 53.5286 bits (127), Expect = 1.125e-6
Identity = 30/83 (36.14%), Postives = 43/83 (51.81%), Query Frame = 3
Query:  657 YSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYTQYSNLCRHKRMSRDCQRKAKCTECGMEFTNGKTYDTH-KQHCLG 902
            Y+C  CGK F  S+ L +H+  H+  +PY C  C +S++  SNL RH R   + ++  KC  C   F      D H K+H  G
Sbjct:  724 YTCRYCGKIFPRSANLTRHLRTHTGEQPYRCKYCDRSFSISSNLQRHVRNIHNKEKPFKCHLCDRCFGQQTNLDRHLKKHENG 806          
BLAST of SMED30024013 vs. Ensembl Human
Match: MECOM (MDS1 and EVI1 complex locus [Source:HGNC Symbol;Acc:HGNC:3498])

HSP 1 Score: 132.109 bits (331), Expect = 2.246e-31
Identity = 56/80 (70.00%), Postives = 67/80 (83.75%), Query Frame = 3
Query: 1512 RYHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIRQ 1751
            RY C  C K FPR+ANL RHLRTHTGEQPY+C +CD+ FSISSN+QRHVRNIHNKE+P+KC  CDR F Q T+LDRH+++
Sbjct:  732 RYTCRYCGKIFPRSANLTRHLRTHTGEQPYRCKYCDRSFSISSNLQRHVRNIHNKEKPFKCHLCDRCFGQQTNLDRHLKK 811          

HSP 2 Score: 119.013 bits (297), Expect = 3.670e-27
Identity = 75/223 (33.63%), Postives = 111/223 (49.78%), Query Frame = 3
Query:  276 QFQCPDCGANFSSRGPLKRHQNALCKNPNGLLIKTRQTIFKHNSVIQRKQVLGTNQNCTT-----TNLSGGSEHRKTSSDYKQ--------SVSFPISKLSSPISNGSNLNLFCDVCNKPCRSQVSLIRH-RKQHQNPNSHYSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYTQYSNLCRHKRMSRDCQRKAKCTECGMEFTNGKTYDTHKQHCLG 902
            Q++C DC   F S+  L  HQ   C  P+       +   +        Q + T Q C        +L    +H  + ++ ++        + ++  + +   +S+ S  +  C+ C K      +L RH R QH    +H +C  CGKTF  SSGLKQH HIHSS KP+ C  C KSYTQ+SNLCRHKRM  DC+ + KC +CG  F+   + + H++ C G
Sbjct:   20 QYRCEDCDQLFESKAELADHQKFPCSTPHSAFSMVEEDFQQKLESENDLQEIHTIQECKECDQVFPDLQSLEKHMLSHTEEREYKCDQCPKAFNWKSNLIRHQMSHDSGKHYECENCAKVFTDPSNLQRHIRSQHVGARAH-ACPECGKTFATSSGLKQHKHIHSSVKPFICEVCHKSYTQFSNLCRHKRMHADCRTQIKCKDCGQMFSTTSSLNKHRRFCEG 241          

HSP 3 Score: 60.8474 bits (146), Expect = 5.318e-9
Identity = 29/76 (38.16%), Postives = 45/76 (59.21%), Query Frame = 3
Query: 1521 CHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIR 1748
            C  C + FP   +L +H+ +HT E+ YKC  C K F+  SN+ RH  + H+  + Y+C  C ++F   ++L RHIR
Sbjct:   77 CKECDQVFPDLQSLEKHMLSHTEEREYKCDQCPKAFNWKSNLIRHQMS-HDSGKHYECENCAKVFTDPSNLQRHIR 151          

HSP 4 Score: 57.7658 bits (138), Expect = 4.946e-8
Identity = 28/77 (36.36%), Postives = 41/77 (53.25%), Query Frame = 3
Query: 1512 RYHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRH 1742
             Y C  C K F   +NL RH  +H   + Y+C  C K F+  SN+QRH+R+ H   R + C +C + F   + L +H
Sbjct:  102 EYKCDQCPKAFNWKSNLIRHQMSHDSGKHYECENCAKVFTDPSNLQRHIRSQHVGARAHACPECGKTFATSSGLKQH 178          

HSP 5 Score: 55.8398 bits (133), Expect = 2.313e-7
Identity = 24/56 (42.86%), Postives = 36/56 (64.29%), Query Frame = 3
Query: 1587 GEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIRQL 1754
            G++ Y C +C K F  S+N+ RH+R  H  E+PY+C  CDR F   ++L RH+R +
Sbjct:  729 GKERYTCRYCGKIFPRSANLTRHLR-THTGEQPYRCKYCDRSFSISSNLQRHVRNI 783          

HSP 6 Score: 53.5286 bits (127), Expect = 1.036e-6
Identity = 30/83 (36.14%), Postives = 43/83 (51.81%), Query Frame = 3
Query:  657 YSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYTQYSNLCRHKRMSRDCQRKAKCTECGMEFTNGKTYDTH-KQHCLG 902
            Y+C  CGK F  S+ L +H+  H+  +PY C  C +S++  SNL RH R   + ++  KC  C   F      D H K+H  G
Sbjct:  733 YTCRYCGKIFPRSANLTRHLRTHTGEQPYRCKYCDRSFSISSNLQRHVRNIHNKEKPFKCHLCDRCFGQQTNLDRHLKKHENG 815          
BLAST of SMED30024013 vs. Ensembl Human
Match: MECOM (MDS1 and EVI1 complex locus [Source:HGNC Symbol;Acc:HGNC:3498])

HSP 1 Score: 132.109 bits (331), Expect = 2.246e-31
Identity = 56/80 (70.00%), Postives = 67/80 (83.75%), Query Frame = 3
Query: 1512 RYHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIRQ 1751
            RY C  C K FPR+ANL RHLRTHTGEQPY+C +CD+ FSISSN+QRHVRNIHNKE+P+KC  CDR F Q T+LDRH+++
Sbjct:  732 RYTCRYCGKIFPRSANLTRHLRTHTGEQPYRCKYCDRSFSISSNLQRHVRNIHNKEKPFKCHLCDRCFGQQTNLDRHLKK 811          

HSP 2 Score: 119.013 bits (297), Expect = 3.670e-27
Identity = 75/223 (33.63%), Postives = 111/223 (49.78%), Query Frame = 3
Query:  276 QFQCPDCGANFSSRGPLKRHQNALCKNPNGLLIKTRQTIFKHNSVIQRKQVLGTNQNCTT-----TNLSGGSEHRKTSSDYKQ--------SVSFPISKLSSPISNGSNLNLFCDVCNKPCRSQVSLIRH-RKQHQNPNSHYSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYTQYSNLCRHKRMSRDCQRKAKCTECGMEFTNGKTYDTHKQHCLG 902
            Q++C DC   F S+  L  HQ   C  P+       +   +        Q + T Q C        +L    +H  + ++ ++        + ++  + +   +S+ S  +  C+ C K      +L RH R QH    +H +C  CGKTF  SSGLKQH HIHSS KP+ C  C KSYTQ+SNLCRHKRM  DC+ + KC +CG  F+   + + H++ C G
Sbjct:   20 QYRCEDCDQLFESKAELADHQKFPCSTPHSAFSMVEEDFQQKLESENDLQEIHTIQECKECDQVFPDLQSLEKHMLSHTEEREYKCDQCPKAFNWKSNLIRHQMSHDSGKHYECENCAKVFTDPSNLQRHIRSQHVGARAH-ACPECGKTFATSSGLKQHKHIHSSVKPFICEVCHKSYTQFSNLCRHKRMHADCRTQIKCKDCGQMFSTTSSLNKHRRFCEG 241          

HSP 3 Score: 60.8474 bits (146), Expect = 5.318e-9
Identity = 29/76 (38.16%), Postives = 45/76 (59.21%), Query Frame = 3
Query: 1521 CHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIR 1748
            C  C + FP   +L +H+ +HT E+ YKC  C K F+  SN+ RH  + H+  + Y+C  C ++F   ++L RHIR
Sbjct:   77 CKECDQVFPDLQSLEKHMLSHTEEREYKCDQCPKAFNWKSNLIRHQMS-HDSGKHYECENCAKVFTDPSNLQRHIR 151          

HSP 4 Score: 57.7658 bits (138), Expect = 4.946e-8
Identity = 28/77 (36.36%), Postives = 41/77 (53.25%), Query Frame = 3
Query: 1512 RYHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRH 1742
             Y C  C K F   +NL RH  +H   + Y+C  C K F+  SN+QRH+R+ H   R + C +C + F   + L +H
Sbjct:  102 EYKCDQCPKAFNWKSNLIRHQMSHDSGKHYECENCAKVFTDPSNLQRHIRSQHVGARAHACPECGKTFATSSGLKQH 178          

HSP 5 Score: 55.8398 bits (133), Expect = 2.313e-7
Identity = 24/56 (42.86%), Postives = 36/56 (64.29%), Query Frame = 3
Query: 1587 GEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIRQL 1754
            G++ Y C +C K F  S+N+ RH+R  H  E+PY+C  CDR F   ++L RH+R +
Sbjct:  729 GKERYTCRYCGKIFPRSANLTRHLR-THTGEQPYRCKYCDRSFSISSNLQRHVRNI 783          

HSP 6 Score: 53.5286 bits (127), Expect = 1.036e-6
Identity = 30/83 (36.14%), Postives = 43/83 (51.81%), Query Frame = 3
Query:  657 YSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYTQYSNLCRHKRMSRDCQRKAKCTECGMEFTNGKTYDTH-KQHCLG 902
            Y+C  CGK F  S+ L +H+  H+  +PY C  C +S++  SNL RH R   + ++  KC  C   F      D H K+H  G
Sbjct:  733 YTCRYCGKIFPRSANLTRHLRTHTGEQPYRCKYCDRSFSISSNLQRHVRNIHNKEKPFKCHLCDRCFGQQTNLDRHLKKHENG 815          
BLAST of SMED30024013 vs. Ensembl Human
Match: MECOM (MDS1 and EVI1 complex locus [Source:HGNC Symbol;Acc:HGNC:3498])

HSP 1 Score: 132.109 bits (331), Expect = 2.257e-31
Identity = 56/80 (70.00%), Postives = 67/80 (83.75%), Query Frame = 3
Query: 1512 RYHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIRQ 1751
            RY C  C K FPR+ANL RHLRTHTGEQPY+C +CD+ FSISSN+QRHVRNIHNKE+P+KC  CDR F Q T+LDRH+++
Sbjct:  911 RYTCRYCGKIFPRSANLTRHLRTHTGEQPYRCKYCDRSFSISSNLQRHVRNIHNKEKPFKCHLCDRCFGQQTNLDRHLKK 990          

HSP 2 Score: 119.013 bits (297), Expect = 3.443e-27
Identity = 75/223 (33.63%), Postives = 111/223 (49.78%), Query Frame = 3
Query:  276 QFQCPDCGANFSSRGPLKRHQNALCKNPNGLLIKTRQTIFKHNSVIQRKQVLGTNQNCTT-----TNLSGGSEHRKTSSDYKQ--------SVSFPISKLSSPISNGSNLNLFCDVCNKPCRSQVSLIRH-RKQHQNPNSHYSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYTQYSNLCRHKRMSRDCQRKAKCTECGMEFTNGKTYDTHKQHCLG 902
            Q++C DC   F S+  L  HQ   C  P+       +   +        Q + T Q C        +L    +H  + ++ ++        + ++  + +   +S+ S  +  C+ C K      +L RH R QH    +H +C  CGKTF  SSGLKQH HIHSS KP+ C  C KSYTQ+SNLCRHKRM  DC+ + KC +CG  F+   + + H++ C G
Sbjct:  208 QYRCEDCDQLFESKAELADHQKFPCSTPHSAFSMVEEDFQQKLESENDLQEIHTIQECKECDQVFPDLQSLEKHMLSHTEEREYKCDQCPKAFNWKSNLIRHQMSHDSGKHYECENCAKVFTDPSNLQRHIRSQHVGARAH-ACPECGKTFATSSGLKQHKHIHSSVKPFICEVCHKSYTQFSNLCRHKRMHADCRTQIKCKDCGQMFSTTSSLNKHRRFCEG 429          

HSP 3 Score: 61.2326 bits (147), Expect = 4.709e-9
Identity = 29/76 (38.16%), Postives = 45/76 (59.21%), Query Frame = 3
Query: 1521 CHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIR 1748
            C  C + FP   +L +H+ +HT E+ YKC  C K F+  SN+ RH  + H+  + Y+C  C ++F   ++L RHIR
Sbjct:  265 CKECDQVFPDLQSLEKHMLSHTEEREYKCDQCPKAFNWKSNLIRHQMS-HDSGKHYECENCAKVFTDPSNLQRHIR 339          

HSP 4 Score: 58.151 bits (139), Expect = 4.148e-8
Identity = 28/77 (36.36%), Postives = 41/77 (53.25%), Query Frame = 3
Query: 1512 RYHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRH 1742
             Y C  C K F   +NL RH  +H   + Y+C  C K F+  SN+QRH+R+ H   R + C +C + F   + L +H
Sbjct:  290 EYKCDQCPKAFNWKSNLIRHQMSHDSGKHYECENCAKVFTDPSNLQRHIRSQHVGARAHACPECGKTFATSSGLKQH 366          

HSP 5 Score: 55.8398 bits (133), Expect = 2.240e-7
Identity = 24/56 (42.86%), Postives = 36/56 (64.29%), Query Frame = 3
Query: 1587 GEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIRQL 1754
            G++ Y C +C K F  S+N+ RH+R  H  E+PY+C  CDR F   ++L RH+R +
Sbjct:  908 GKERYTCRYCGKIFPRSANLTRHLRT-HTGEQPYRCKYCDRSFSISSNLQRHVRNI 962          

HSP 6 Score: 53.9138 bits (128), Expect = 9.110e-7
Identity = 29/80 (36.25%), Postives = 42/80 (52.50%), Query Frame = 3
Query:  657 YSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYTQYSNLCRHKRMSRDCQRKAKCTECGMEFTNGKTYDTH-KQH 893
            Y+C  CGK F  S+ L +H+  H+  +PY C  C +S++  SNL RH R   + ++  KC  C   F      D H K+H
Sbjct:  912 YTCRYCGKIFPRSANLTRHLRTHTGEQPYRCKYCDRSFSISSNLQRHVRNIHNKEKPFKCHLCDRCFGQQTNLDRHLKKH 991          
BLAST of SMED30024013 vs. Ensembl Celegans
Match: egl-43 (pep chromosome:WBcel235:II:9960333:9962344:1 gene:WBGene00001207.1 transcript:R53.3b.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:egl-43)

HSP 1 Score: 99.3673 bits (246), Expect = 1.374e-22
Identity = 42/55 (76.36%), Postives = 47/55 (85.45%), Query Frame = 3
Query: 1512 RYHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNK 1676
            RY C  C K FPR+ANL RHLRTHTGEQPYKC +C++ FSISSN+QRHVRNIHNK
Sbjct:  222 RYTCKFCQKVFPRSANLTRHLRTHTGEQPYKCQYCERSFSISSNLQRHVRNIHNK 276          

HSP 2 Score: 56.225 bits (134), Expect = 2.859e-8
Identity = 24/52 (46.15%), Postives = 33/52 (63.46%), Query Frame = 3
Query: 1599 YKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIRQL 1754
            Y C FC K F  S+N+ RH+R  H  E+PYKC  C+R F   ++L RH+R +
Sbjct:  223 YTCKFCQKVFPRSANLTRHLRT-HTGEQPYKCQYCERSFSISSNLQRHVRNI 273          

HSP 3 Score: 48.521 bits (114), Expect = 8.348e-6
Identity = 20/49 (40.82%), Postives = 30/49 (61.22%), Query Frame = 3
Query:  657 YSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYTQYSNLCRHKR 803
            Y+C  C K F  S+ L +H+  H+  +PY C  C++S++  SNL RH R
Sbjct:  223 YTCKFCQKVFPRSANLTRHLRTHTGEQPYKCQYCERSFSISSNLQRHVR 271          
BLAST of SMED30024013 vs. Ensembl Celegans
Match: egl-43 (pep chromosome:WBcel235:II:9955185:9962344:1 gene:WBGene00001207.1 transcript:R53.3a.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:egl-43)

HSP 1 Score: 98.5969 bits (244), Expect = 1.855e-21
Identity = 42/55 (76.36%), Postives = 47/55 (85.45%), Query Frame = 3
Query: 1512 RYHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNK 1676
            RY C  C K FPR+ANL RHLRTHTGEQPYKC +C++ FSISSN+QRHVRNIHNK
Sbjct:  443 RYTCKFCQKVFPRSANLTRHLRTHTGEQPYKCQYCERSFSISSNLQRHVRNIHNK 497          

HSP 2 Score: 73.559 bits (179), Expect = 1.511e-13
Identity = 37/84 (44.05%), Postives = 48/84 (57.14%), Query Frame = 3
Query:  645 PNSHYSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYTQYSNLCRHKRMSRDCQRKAKCTECGMEFTNGKTYDTHKQHC 896
            P+  + C VC K+F  +SGLKQH HIH S KP+ C  C KSYTQ+SNLCRH+R+  D      C  C  +  +      H+  C
Sbjct:  155 PDGSHKCGVCPKSFSSASGLKQHSHIHCSLKPFRCHLCPKSYTQFSNLCRHRRVHSD---GWTCPTCQSQMPSQAALTKHRPVC 235          

HSP 3 Score: 55.0694 bits (131), Expect = 1.082e-7
Identity = 24/52 (46.15%), Postives = 33/52 (63.46%), Query Frame = 3
Query: 1599 YKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIRQL 1754
            Y C FC K F  S+N+ RH+R  H  E+PYKC  C+R F   ++L RH+R +
Sbjct:  444 YTCKFCQKVFPRSANLTRHLRT-HTGEQPYKCQYCERSFSISSNLQRHVRNI 494          
BLAST of SMED30024013 vs. Ensembl Celegans
Match: F47E1.3 (pep chromosome:WBcel235:X:9046213:9054885:1 gene:WBGene00018567.1 transcript:F47E1.3.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:F47E1.3)

HSP 1 Score: 82.8037 bits (203), Expect = 2.239e-16
Identity = 35/77 (45.45%), Postives = 55/77 (71.43%), Query Frame = 3
Query: 1521 CHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIRQ 1751
            C +C K+F ++++LN+HLR H+GE+PYKC+FC K F+ SS ++ HVR  H+ E+P+KC  C + F    + D H+R+
Sbjct:  526 CSVCQKSFSQSSSLNKHLRVHSGERPYKCSFCPKAFTASSILRTHVRQ-HSGEKPFKCAHCGKAFASHAAHDSHVRR 601          

HSP 2 Score: 62.3882 bits (150), Expect = 6.833e-10
Identity = 39/114 (34.21%), Postives = 56/114 (49.12%), Query Frame = 3
Query:  546 ISNGSNLNLFCDVCNKPCRSQVSLIRHRKQ-HQNPNSHYSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYTQYSNLCRHKRMSRDCQRKAKCTECGMEFTNGKTYDTH 884
            + NG N    C +CN+    +  L  H    H+N   H  C VC K+F  SS L +H+ +HS  +PY C  C K++T  S L  H R     ++  KC  CG  F +   +D+H
Sbjct:  488 VDNG-NRKFPCTICNRSFEKRDRLRIHILHVHENHRPHV-CSVCQKSFSQSSSLNKHLRVHSGERPYKCSFCPKAFTASSILRTHVR-QHSGEKPFKCAHCGKAFASHAAHDSH 598          

HSP 3 Score: 50.447 bits (119), Expect = 3.581e-6
Identity = 22/52 (42.31%), Postives = 30/52 (57.69%), Query Frame = 3
Query: 1515 YHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIH 1670
            Y C  C K F  ++ L  H+R H+GE+P+KCA C K F+  +    HVR  H
Sbjct:  552 YKCSFCPKAFTASSILRTHVRQHSGEKPFKCAHCGKAFASHAAHDSHVRRSH 603          

HSP 4 Score: 49.2914 bits (116), Expect = 6.380e-6
Identity = 22/82 (26.83%), Postives = 42/82 (51.22%), Query Frame = 3
Query: 1515 YHCHLCLKTFPRAANLNRHLR----THTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIR 1748
            + C  C K F      ++HL+       G + + C  C++ F     ++ H+ ++H   RP+ C+ C + F Q +SL++H+R
Sbjct:  463 FSCERCGKVFSYEYYRDKHLKYTRCVDNGNRKFPCTICNRSFEKRDRLRIHILHVHENHRPHVCSVCQKSFSQSSSLNKHLR 544          
BLAST of SMED30024013 vs. Ensembl Celegans
Match: che-1 (Transcription factor che-1 [Source:UniProtKB/Swiss-Prot;Acc:Q966L8])

HSP 1 Score: 75.8702 bits (185), Expect = 1.707e-15
Identity = 34/78 (43.59%), Postives = 49/78 (62.82%), Query Frame = 3
Query: 1515 YHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIR 1748
            + C  C K F +AANL  H R HTGE+P+ C  C++ FS SS++  H R  H  ERPY C +C++ F   ++L +H+R
Sbjct:  107 FRCQTCGKAFSQAANLTAHKRIHTGEKPFMCPVCNRPFSQSSSLVTH-RRTHTGERPYPCAQCEKAFTDSSTLTKHLR 183          

HSP 2 Score: 68.1662 bits (165), Expect = 9.761e-13
Identity = 32/78 (41.03%), Postives = 50/78 (64.10%), Query Frame = 3
Query: 1515 YHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIR 1748
            + C +C + F ++++L  H RTHTGE+PY CA C+K F+ SS + +H+R  H   +PY C+ C   F Q  +L RH++
Sbjct:  135 FMCPVCNRPFSQSSSLVTHRRTHTGERPYPCAQCEKAFTDSSTLTKHLR-THTGHKPYVCSICMMKFTQSGNLHRHMK 211          

HSP 3 Score: 60.8474 bits (146), Expect = 2.560e-10
Identity = 31/78 (39.74%), Postives = 40/78 (51.28%), Query Frame = 3
Query:  570 LFCDVCNKPCRSQVSLIRHRKQHQNPNSHYSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYTQYSNLCRHKR 803
              C VCN+P     SL+ HR+ H      Y C  C K F  SS L +H+  H+ +KPY C  C   +TQ  NL RH +
Sbjct:  135 FMCPVCNRPFSQSSSLVTHRRTHTGERP-YPCAQCEKAFTDSSTLTKHLRTHTGHKPYVCSICMMKFTQSGNLHRHMK 211          

HSP 4 Score: 56.225 bits (134), Expect = 8.574e-9
Identity = 31/109 (28.44%), Postives = 50/109 (45.87%), Query Frame = 3
Query:  534 LSSPISNGSNLNLFCDVCNKPCRSQVSLIRHRKQHQNPNSHYSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYTQYSNLCRHKRMSRDCQRKAKCTECGMEFT 860
            +  P++  +     C  C K      +L  H++ H      + C VC + F  SS L  H   H+  +PY C  C+K++T  S L +H R +    +   C+ C M+FT
Sbjct:   95 VQRPMARSTPKPFRCQTCGKAFSQAANLTAHKRIHTGEKP-FMCPVCNRPFSQSSSLVTHRRTHTGERPYPCAQCEKAFTDSSTLTKHLR-THTGHKPYVCSICMMKFT 201          
BLAST of SMED30024013 vs. Ensembl Celegans
Match: che-1 (Transcription factor che-1 [Source:UniProtKB/Swiss-Prot;Acc:Q966L8])

HSP 1 Score: 77.0258 bits (188), Expect = 2.372e-15
Identity = 34/78 (43.59%), Postives = 49/78 (62.82%), Query Frame = 3
Query: 1515 YHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIR 1748
            + C  C K F +AANL  H R HTGE+P+ C  C++ FS SS++  H R  H  ERPY C +C++ F   ++L +H+R
Sbjct:  166 FRCQTCGKAFSQAANLTAHKRIHTGEKPFMCPVCNRPFSQSSSLVTH-RRTHTGERPYPCAQCEKAFTDSSTLTKHLR 242          

HSP 2 Score: 68.9366 bits (167), Expect = 1.067e-12
Identity = 32/78 (41.03%), Postives = 50/78 (64.10%), Query Frame = 3
Query: 1515 YHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIR 1748
            + C +C + F ++++L  H RTHTGE+PY CA C+K F+ SS + +H+R  H   +PY C+ C   F Q  +L RH++
Sbjct:  194 FMCPVCNRPFSQSSSLVTHRRTHTGERPYPCAQCEKAFTDSSTLTKHLR-THTGHKPYVCSICMMKFTQSGNLHRHMK 270          

HSP 3 Score: 61.6178 bits (148), Expect = 2.986e-10
Identity = 31/78 (39.74%), Postives = 40/78 (51.28%), Query Frame = 3
Query:  570 LFCDVCNKPCRSQVSLIRHRKQHQNPNSHYSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYTQYSNLCRHKR 803
              C VCN+P     SL+ HR+ H      Y C  C K F  SS L +H+  H+ +KPY C  C   +TQ  NL RH +
Sbjct:  194 FMCPVCNRPFSQSSSLVTHRRTHTGERP-YPCAQCEKAFTDSSTLTKHLRTHTGHKPYVCSICMMKFTQSGNLHRHMK 270          

HSP 4 Score: 56.6102 bits (135), Expect = 1.388e-8
Identity = 31/109 (28.44%), Postives = 50/109 (45.87%), Query Frame = 3
Query:  534 LSSPISNGSNLNLFCDVCNKPCRSQVSLIRHRKQHQNPNSHYSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYTQYSNLCRHKRMSRDCQRKAKCTECGMEFT 860
            +  P++  +     C  C K      +L  H++ H      + C VC + F  SS L  H   H+  +PY C  C+K++T  S L +H R +    +   C+ C M+FT
Sbjct:  154 VQRPMARSTPKPFRCQTCGKAFSQAANLTAHKRIHTGEKP-FMCPVCNRPFSQSSSLVTHRRTHTGERPYPCAQCEKAFTDSSTLTKHLR-THTGHKPYVCSICMMKFT 260          

HSP 5 Score: 47.7506 bits (112), Expect = 9.592e-6
Identity = 22/52 (42.31%), Postives = 32/52 (61.54%), Query Frame = 3
Query: 1593 QPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIR 1748
            +P++C  C K FS ++N+  H R IH  E+P+ C  C+R F Q +SL  H R
Sbjct:  164 KPFRCQTCGKAFSQAANLTAHKR-IHTGEKPFMCPVCNRPFSQSSSLVTHRR 214          
BLAST of SMED30024013 vs. Ensembl Fly
Match: ham (gene:FBgn0045852 transcript:FBtr0332016)

HSP 1 Score: 134.806 bits (338), Expect = 5.633e-33
Identity = 56/80 (70.00%), Postives = 66/80 (82.50%), Query Frame = 3
Query: 1512 RYHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIRQ 1751
            RY C  C K FPR+ANL RHLRTHTGEQPY C +CD+ FSISSN+QRHVRNIHNKERP++C  CDR F Q T+LDRH+++
Sbjct:  459 RYSCKFCGKVFPRSANLTRHLRTHTGEQPYPCKYCDRAFSISSNLQRHVRNIHNKERPFRCELCDRSFGQQTNLDRHVKK 538          

HSP 2 Score: 58.5362 bits (140), Expect = 1.266e-8
Identity = 29/80 (36.25%), Postives = 42/80 (52.50%), Query Frame = 3
Query:  657 YSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYTQYSNLCRHKRMSRDCQRKAKCTECGMEFTNGKTYDTH-KQH 893
            YSC  CGK F  S+ L +H+  H+  +PY C  C ++++  SNL RH R   + +R  +C  C   F      D H K+H
Sbjct:  460 YSCKFCGKVFPRSANLTRHLRTHTGEQPYPCKYCDRAFSISSNLQRHVRNIHNKERPFRCELCDRSFGQQTNLDRHVKKH 539          

HSP 3 Score: 55.8398 bits (133), Expect = 8.392e-8
Identity = 24/52 (46.15%), Postives = 32/52 (61.54%), Query Frame = 3
Query: 1599 YKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIRQL 1754
            Y C FC K F  S+N+ RH+R  H  E+PY C  CDR F   ++L RH+R +
Sbjct:  460 YSCKFCGKVFPRSANLTRHLR-THTGEQPYPCKYCDRAFSISSNLQRHVRNI 510          
BLAST of SMED30024013 vs. Ensembl Fly
Match: CG10348 (gene:FBgn0032707 transcript:FBtr0343112)

HSP 1 Score: 131.724 bits (330), Expect = 2.945e-32
Identity = 56/80 (70.00%), Postives = 67/80 (83.75%), Query Frame = 3
Query: 1512 RYHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIRQ 1751
            RY C  C K FPR+ANL RHLRTHTGEQPYKC +C++ FSISSN+QRHVRNIHNKERP+KC  C+R F Q T+LDRH+++
Sbjct:  310 RYTCKFCGKVFPRSANLTRHLRTHTGEQPYKCKYCERSFSISSNLQRHVRNIHNKERPFKCEICERCFGQQTNLDRHLKK 389          

HSP 2 Score: 58.5362 bits (140), Expect = 1.239e-8
Identity = 30/80 (37.50%), Postives = 43/80 (53.75%), Query Frame = 3
Query:  657 YSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYTQYSNLCRHKRMSRDCQRKAKCTECGMEFTNGKTYDTH-KQH 893
            Y+C  CGK F  S+ L +H+  H+  +PY C  C++S++  SNL RH R   + +R  KC  C   F      D H K+H
Sbjct:  311 YTCKFCGKVFPRSANLTRHLRTHTGEQPYKCKYCERSFSISSNLQRHVRNIHNKERPFKCEICERCFGQQTNLDRHLKKH 390          

HSP 3 Score: 55.0694 bits (131), Expect = 1.545e-7
Identity = 24/52 (46.15%), Postives = 33/52 (63.46%), Query Frame = 3
Query: 1599 YKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIRQL 1754
            Y C FC K F  S+N+ RH+R  H  E+PYKC  C+R F   ++L RH+R +
Sbjct:  311 YTCKFCGKVFPRSANLTRHLRT-HTGEQPYKCKYCERSFSISSNLQRHVRNI 361          
BLAST of SMED30024013 vs. Ensembl Fly
Match: CG10348 (gene:FBgn0032707 transcript:FBtr0301647)

HSP 1 Score: 131.724 bits (330), Expect = 2.945e-32
Identity = 56/80 (70.00%), Postives = 67/80 (83.75%), Query Frame = 3
Query: 1512 RYHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIRQ 1751
            RY C  C K FPR+ANL RHLRTHTGEQPYKC +C++ FSISSN+QRHVRNIHNKERP+KC  C+R F Q T+LDRH+++
Sbjct:  310 RYTCKFCGKVFPRSANLTRHLRTHTGEQPYKCKYCERSFSISSNLQRHVRNIHNKERPFKCEICERCFGQQTNLDRHLKK 389          

HSP 2 Score: 58.5362 bits (140), Expect = 1.239e-8
Identity = 30/80 (37.50%), Postives = 43/80 (53.75%), Query Frame = 3
Query:  657 YSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYTQYSNLCRHKRMSRDCQRKAKCTECGMEFTNGKTYDTH-KQH 893
            Y+C  CGK F  S+ L +H+  H+  +PY C  C++S++  SNL RH R   + +R  KC  C   F      D H K+H
Sbjct:  311 YTCKFCGKVFPRSANLTRHLRTHTGEQPYKCKYCERSFSISSNLQRHVRNIHNKERPFKCEICERCFGQQTNLDRHLKKH 390          

HSP 3 Score: 55.0694 bits (131), Expect = 1.545e-7
Identity = 24/52 (46.15%), Postives = 33/52 (63.46%), Query Frame = 3
Query: 1599 YKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIRQL 1754
            Y C FC K F  S+N+ RH+R  H  E+PYKC  C+R F   ++L RH+R +
Sbjct:  311 YTCKFCGKVFPRSANLTRHLRT-HTGEQPYKCKYCERSFSISSNLQRHVRNI 361          
BLAST of SMED30024013 vs. Ensembl Fly
Match: ham (gene:FBgn0045852 transcript:FBtr0305621)

HSP 1 Score: 132.494 bits (332), Expect = 7.292e-32
Identity = 56/80 (70.00%), Postives = 66/80 (82.50%), Query Frame = 3
Query: 1512 RYHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIRQ 1751
            RY C  C K FPR+ANL RHLRTHTGEQPY C +CD+ FSISSN+QRHVRNIHNKERP++C  CDR F Q T+LDRH+++
Sbjct:  816 RYSCKFCGKVFPRSANLTRHLRTHTGEQPYPCKYCDRAFSISSNLQRHVRNIHNKERPFRCELCDRSFGQQTNLDRHVKK 895          

HSP 2 Score: 100.138 bits (248), Expect = 1.542e-21
Identity = 52/117 (44.44%), Postives = 66/117 (56.41%), Query Frame = 3
Query:  570 LFCDVCNKPCRSQVSLIRHRKQHQNPNSHYSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYTQYSNLCRHKRMSRDCQRKAKCTECGMEFTNGKTYDTHKQHCLGLDPSGY 920
            +FCD  N        L RH + +      + C  CGKTF  SSGLKQH HIHSS KP++C  C K+YTQ+SNLCRHKRM   C+ + KC +C   F+   +   HK+ C    P  Y
Sbjct:  288 VFCDPSN--------LQRHIRAYHVGARCHPCPECGKTFGTSSGLKQHQHIHSSVKPFACEVCSKAYTQFSNLCRHKRMHATCRMQIKCDKCNQSFSTLTSLTKHKKFCDSTGPGPY 396          

HSP 3 Score: 58.151 bits (139), Expect = 2.195e-8
Identity = 29/80 (36.25%), Postives = 42/80 (52.50%), Query Frame = 3
Query:  657 YSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYTQYSNLCRHKRMSRDCQRKAKCTECGMEFTNGKTYDTH-KQH 893
            YSC  CGK F  S+ L +H+  H+  +PY C  C ++++  SNL RH R   + +R  +C  C   F      D H K+H
Sbjct:  817 YSCKFCGKVFPRSANLTRHLRTHTGEQPYPCKYCDRAFSISSNLQRHVRNIHNKERPFRCELCDRSFGQQTNLDRHVKKH 896          

HSP 4 Score: 56.9954 bits (136), Expect = 5.043e-8
Identity = 29/80 (36.25%), Postives = 46/80 (57.50%), Query Frame = 3
Query: 1512 RYHCHLCLKTFPRAANLNRHLRT-HTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIR 1748
            +Y C  C K F   +NL RH+R  H G + + C  C K F  SS +++H ++IH+  +P+ C  C + + Q ++L RH R
Sbjct:  279 KYSCENCSKVFCDPSNLQRHIRAYHVGARCHPCPECGKTFGTSSGLKQH-QHIHSSVKPFACEVCSKAYTQFSNLCRHKR 357          

HSP 5 Score: 55.0694 bits (131), Expect = 1.971e-7
Identity = 24/52 (46.15%), Postives = 32/52 (61.54%), Query Frame = 3
Query: 1599 YKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIRQL 1754
            Y C FC K F  S+N+ RH+R  H  E+PY C  CDR F   ++L RH+R +
Sbjct:  817 YSCKFCGKVFPRSANLTRHLR-THTGEQPYPCKYCDRAFSISSNLQRHVRNI 867          

HSP 6 Score: 51.2174 bits (121), Expect = 2.928e-6
Identity = 28/79 (35.44%), Postives = 42/79 (53.16%), Query Frame = 3
Query: 1521 CHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIRQLC 1757
            C  C KTF  ++ L +H   H+  +P+ C  C K ++  SN+ RH R         KC KC++ F  LTSL +H ++ C
Sbjct:  311 CPECGKTFGTSSGLKQHQHIHSSVKPFACEVCSKAYTQFSNLCRHKRMHATCRMQIKCDKCNQSFSTLTSLTKH-KKFC 388          
BLAST of SMED30024013 vs. Ensembl Fly
Match: ham (gene:FBgn0045852 transcript:FBtr0332015)

HSP 1 Score: 132.494 bits (332), Expect = 7.292e-32
Identity = 56/80 (70.00%), Postives = 66/80 (82.50%), Query Frame = 3
Query: 1512 RYHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIRQ 1751
            RY C  C K FPR+ANL RHLRTHTGEQPY C +CD+ FSISSN+QRHVRNIHNKERP++C  CDR F Q T+LDRH+++
Sbjct:  816 RYSCKFCGKVFPRSANLTRHLRTHTGEQPYPCKYCDRAFSISSNLQRHVRNIHNKERPFRCELCDRSFGQQTNLDRHVKK 895          

HSP 2 Score: 100.138 bits (248), Expect = 1.542e-21
Identity = 52/117 (44.44%), Postives = 66/117 (56.41%), Query Frame = 3
Query:  570 LFCDVCNKPCRSQVSLIRHRKQHQNPNSHYSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYTQYSNLCRHKRMSRDCQRKAKCTECGMEFTNGKTYDTHKQHCLGLDPSGY 920
            +FCD  N        L RH + +      + C  CGKTF  SSGLKQH HIHSS KP++C  C K+YTQ+SNLCRHKRM   C+ + KC +C   F+   +   HK+ C    P  Y
Sbjct:  288 VFCDPSN--------LQRHIRAYHVGARCHPCPECGKTFGTSSGLKQHQHIHSSVKPFACEVCSKAYTQFSNLCRHKRMHATCRMQIKCDKCNQSFSTLTSLTKHKKFCDSTGPGPY 396          

HSP 3 Score: 58.151 bits (139), Expect = 2.195e-8
Identity = 29/80 (36.25%), Postives = 42/80 (52.50%), Query Frame = 3
Query:  657 YSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYTQYSNLCRHKRMSRDCQRKAKCTECGMEFTNGKTYDTH-KQH 893
            YSC  CGK F  S+ L +H+  H+  +PY C  C ++++  SNL RH R   + +R  +C  C   F      D H K+H
Sbjct:  817 YSCKFCGKVFPRSANLTRHLRTHTGEQPYPCKYCDRAFSISSNLQRHVRNIHNKERPFRCELCDRSFGQQTNLDRHVKKH 896          

HSP 4 Score: 56.9954 bits (136), Expect = 5.043e-8
Identity = 29/80 (36.25%), Postives = 46/80 (57.50%), Query Frame = 3
Query: 1512 RYHCHLCLKTFPRAANLNRHLRT-HTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIR 1748
            +Y C  C K F   +NL RH+R  H G + + C  C K F  SS +++H ++IH+  +P+ C  C + + Q ++L RH R
Sbjct:  279 KYSCENCSKVFCDPSNLQRHIRAYHVGARCHPCPECGKTFGTSSGLKQH-QHIHSSVKPFACEVCSKAYTQFSNLCRHKR 357          

HSP 5 Score: 55.0694 bits (131), Expect = 1.971e-7
Identity = 24/52 (46.15%), Postives = 32/52 (61.54%), Query Frame = 3
Query: 1599 YKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIRQL 1754
            Y C FC K F  S+N+ RH+R  H  E+PY C  CDR F   ++L RH+R +
Sbjct:  817 YSCKFCGKVFPRSANLTRHLR-THTGEQPYPCKYCDRAFSISSNLQRHVRNI 867          

HSP 6 Score: 51.2174 bits (121), Expect = 2.928e-6
Identity = 28/79 (35.44%), Postives = 42/79 (53.16%), Query Frame = 3
Query: 1521 CHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIRQLC 1757
            C  C KTF  ++ L +H   H+  +P+ C  C K ++  SN+ RH R         KC KC++ F  LTSL +H ++ C
Sbjct:  311 CPECGKTFGTSSGLKQHQHIHSSVKPFACEVCSKAYTQFSNLCRHKRMHATCRMQIKCDKCNQSFSTLTSLTKH-KKFC 388          
BLAST of SMED30024013 vs. Ensembl Zebrafish
Match: si:ch211-263k4.2 (si:ch211-263k4.2 [Source:ZFIN;Acc:ZDB-GENE-081104-207])

HSP 1 Score: 129.798 bits (325), Expect = 6.116e-31
Identity = 55/80 (68.75%), Postives = 67/80 (83.75%), Query Frame = 3
Query: 1512 RYHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIRQ 1751
            RY C  C K FPR+ANL RHLRTHTGEQPY+C +CD+ FSISSN+QRHVRNIHNKE+P+KC  C+R F Q T+LDRH+++
Sbjct:  589 RYTCRYCGKIFPRSANLTRHLRTHTGEQPYRCKYCDRSFSISSNLQRHVRNIHNKEKPFKCHLCNRCFGQQTNLDRHLKK 668          

HSP 2 Score: 61.6178 bits (148), Expect = 2.101e-9
Identity = 25/49 (51.02%), Postives = 34/49 (69.39%), Query Frame = 3
Query:  756 CQKSYTQYSNLCRHKRMSRDCQRKAKCTECGMEFTNGKTYDTHKQHCLG 902
            C KSYTQ+SNLCRHKRM  DC+ + KC +CG  F+   + + H++ C G
Sbjct:    9 CHKSYTQFSNLCRHKRMHADCRTQIKCKDCGQMFSTTSSLNKHRRFCEG 57          

HSP 3 Score: 56.6102 bits (135), Expect = 8.299e-8
Identity = 24/56 (42.86%), Postives = 36/56 (64.29%), Query Frame = 3
Query: 1587 GEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIRQL 1754
            G++ Y C +C K F  S+N+ RH+R  H  E+PY+C  CDR F   ++L RH+R +
Sbjct:  586 GKERYTCRYCGKIFPRSANLTRHLR-THTGEQPYRCKYCDRSFSISSNLQRHVRNI 640          

HSP 4 Score: 53.9138 bits (128), Expect = 4.777e-7
Identity = 29/80 (36.25%), Postives = 42/80 (52.50%), Query Frame = 3
Query:  657 YSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYTQYSNLCRHKRMSRDCQRKAKCTECGMEFTNGKTYDTH-KQH 893
            Y+C  CGK F  S+ L +H+  H+  +PY C  C +S++  SNL RH R   + ++  KC  C   F      D H K+H
Sbjct:  590 YTCRYCGKIFPRSANLTRHLRTHTGEQPYRCKYCDRSFSISSNLQRHVRNIHNKEKPFKCHLCNRCFGQQTNLDRHLKKH 669          
BLAST of SMED30024013 vs. Ensembl Zebrafish
Match: si:ch211-263k4.2 (si:ch211-263k4.2 [Source:ZFIN;Acc:ZDB-GENE-081104-207])

HSP 1 Score: 128.642 bits (322), Expect = 1.530e-30
Identity = 55/80 (68.75%), Postives = 67/80 (83.75%), Query Frame = 3
Query: 1512 RYHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIRQ 1751
            RY C  C K FPR+ANL RHLRTHTGEQPY+C +CD+ FSISSN+QRHVRNIHNKE+P+KC  C+R F Q T+LDRH+++
Sbjct:  710 RYTCRYCGKIFPRSANLTRHLRTHTGEQPYRCKYCDRSFSISSNLQRHVRNIHNKEKPFKCHLCNRCFGQQTNLDRHLKK 789          

HSP 2 Score: 113.62 bits (283), Expect = 8.974e-26
Identity = 59/120 (49.17%), Postives = 76/120 (63.33%), Query Frame = 3
Query:  546 ISNGSNLNLFCDVCNKPCRSQVSLIRH-RKQHQNPNSHYSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYTQYSNLCRHKRMSRDCQRKAKCTECGMEFTNGKTYDTHKQHCLG 902
            +S+ S     C+ C+K      +L RH R QH    +H +C  CGKTF  SSGLKQH HIHSS KP+SC  C KSYTQ+SNLCRHKRM  DC+ + KC +CG  F+   + + H++ C G
Sbjct:   60 MSHDSGKRFECENCDKVFTDPSNLQRHIRSQHVGARAH-TCPECGKTFATSSGLKQHKHIHSSVKPFSCEVCHKSYTQFSNLCRHKRMHADCRTQIKCKDCGQMFSTTSSLNKHRRFCEG 178          

HSP 3 Score: 62.7734 bits (151), Expect = 8.960e-10
Identity = 30/78 (38.46%), Postives = 46/78 (58.97%), Query Frame = 3
Query: 1515 YHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIR 1748
            + C  C K FP   +L +H+  HT E+ YKC  C K F+  SN+ RH  + H+  + ++C  CD++F   ++L RHIR
Sbjct:   12 HECKDCEKIFPSEYSLGQHMIVHTEEREYKCDQCPKAFNWKSNLIRHQMS-HDSGKRFECENCDKVFTDPSNLQRHIR 88          

HSP 4 Score: 62.003 bits (149), Expect = 1.767e-9
Identity = 39/104 (37.50%), Postives = 44/104 (42.31%), Query Frame = 3
Query:  576 CDVCNKPCRSQVSLIRHRKQHQNPNSHYSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYTQYSNLCRHKRMSRDCQRKAKCTECGMEFTNGKTYDTHK 887
            C  C K   S+ SL +H   H      Y CD C K F   S L +H   H S K + C NC K +T  SNL RH R      R   C ECG  F        HK
Sbjct:   14 CKDCEKIFPSEYSLGQHMIVHTE-EREYKCDQCPKAFNWKSNLIRHQMSHDSGKRFECENCDKVFTDPSNLQRHIRSQHVGARAHTCPECGKTFATSSGLKQHK 116          

HSP 5 Score: 59.6918 bits (143), Expect = 7.548e-9
Identity = 29/80 (36.25%), Postives = 48/80 (60.00%), Query Frame = 3
Query: 1512 RYHCHLCLKTFPRAANLNRHLRT-HTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIR 1748
            R+ C  C K F   +NL RH+R+ H G + + C  C K F+ SS +++H ++IH+  +P+ C  C + + Q ++L RH R
Sbjct:   67 RFECENCDKVFTDPSNLQRHIRSQHVGARAHTCPECGKTFATSSGLKQH-KHIHSSVKPFSCEVCHKSYTQFSNLCRHKR 145          

HSP 6 Score: 59.3066 bits (142), Expect = 1.083e-8
Identity = 28/77 (36.36%), Postives = 42/77 (54.55%), Query Frame = 3
Query: 1512 RYHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRH 1742
             Y C  C K F   +NL RH  +H   + ++C  CDK F+  SN+QRH+R+ H   R + C +C + F   + L +H
Sbjct:   39 EYKCDQCPKAFNWKSNLIRHQMSHDSGKRFECENCDKVFTDPSNLQRHIRSQHVGARAHTCPECGKTFATSSGLKQH 115          

HSP 7 Score: 55.8398 bits (133), Expect = 1.239e-7
Identity = 24/56 (42.86%), Postives = 36/56 (64.29%), Query Frame = 3
Query: 1587 GEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIRQL 1754
            G++ Y C +C K F  S+N+ RH+R  H  E+PY+C  CDR F   ++L RH+R +
Sbjct:  707 GKERYTCRYCGKIFPRSANLTRHLR-THTGEQPYRCKYCDRSFSISSNLQRHVRNI 761          

HSP 8 Score: 53.5286 bits (127), Expect = 6.830e-7
Identity = 27/76 (35.53%), Postives = 39/76 (51.32%), Query Frame = 3
Query:  657 YSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYTQYSNLCRHKRMSRDCQRKAKCTECGMEFTNGKTYDTH 884
            Y+C  CGK F  S+ L +H+  H+  +PY C  C +S++  SNL RH R   + ++  KC  C   F      D H
Sbjct:  711 YTCRYCGKIFPRSANLTRHLRTHTGEQPYRCKYCDRSFSISSNLQRHVRNIHNKEKPFKCHLCNRCFGQQTNLDRH 786          

HSP 9 Score: 50.8322 bits (120), Expect = 4.698e-6
Identity = 26/76 (34.21%), Postives = 37/76 (48.68%), Query Frame = 3
Query:  657 YSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYTQYSNLCRHKRMSRDCQRKAKCTECGMEFTNGKTYDTH 884
            + C  C K F     L QHM +H+  + Y C  C K++   SNL RH+ MS D  ++ +C  C   FT+      H
Sbjct:   12 HECKDCEKIFPSEYSLGQHMIVHTEEREYKCDQCPKAFNWKSNLIRHQ-MSHDSGKRFECENCDKVFTDPSNLQRH 86          
BLAST of SMED30024013 vs. Ensembl Zebrafish
Match: mecom (MDS1 and EVI1 complex locus [Source:ZFIN;Acc:ZDB-GENE-050208-123])

HSP 1 Score: 105.145 bits (261), Expect = 4.274e-23
Identity = 59/129 (45.74%), Postives = 78/129 (60.47%), Query Frame = 3
Query:  546 ISNGSNLNLFCDVCNKPCRSQVS-LIRH-RKQHQNPNSHYSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLN--------CQKSYTQYSNLCRHKRMSRDCQRKAKCTECGMEFTNGKTYDTHKQHCLG 902
            +S+ S  +  C+ C+K   +  S L RH R QH    +H +C  CGKTF  SSGLKQH HIHSS KP+ C +        C KSYTQ+SNLCRHKRM  DC+ + KC +CG  F+   + + H++ C G
Sbjct:    1 MSHDSGKHYECENCSKQVFTDPSNLQRHIRSQHVGARAH-ACPDCGKTFATSSGLKQHKHIHSSVKPFICKSLRPFICEVCHKSYTQFSNLCRHKRMHADCRTQIKCKDCGQMFSTTSSLNKHRRFCEG 128          
BLAST of SMED30024013 vs. Ensembl Zebrafish
Match: znf1080 (zinc finger protein 1080 [Source:ZFIN;Acc:ZDB-GENE-131125-96])

HSP 1 Score: 86.2705 bits (212), Expect = 1.700e-18
Identity = 39/78 (50.00%), Postives = 55/78 (70.51%), Query Frame = 3
Query: 1515 YHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIR 1748
            + C  C K+F R+ +LN H+RTHTGE+P+ C  C K FSISS++ RH+R IH  E+P+ CT+C + F Q +SL  H+R
Sbjct:  121 FTCTQCGKSFNRSEHLNEHMRTHTGEKPFTCIQCGKIFSISSSLNRHMR-IHTGEKPFTCTQCGKSFSQSSSLKEHMR 197          

HSP 2 Score: 80.4925 bits (197), Expect = 1.651e-16
Identity = 37/78 (47.44%), Postives = 53/78 (67.95%), Query Frame = 3
Query: 1515 YHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIR 1748
            + C  C K F  +++LNRH+R HTGE+P+ C  C K FS SS+++ H+R IH  E+P+ CT+C + F Q +SL  HIR
Sbjct:  149 FTCIQCGKIFSISSSLNRHMRIHTGEKPFTCTQCGKSFSQSSSLKEHMR-IHTGEKPFTCTQCGKSFSQSSSLKEHIR 225          

HSP 3 Score: 73.1738 bits (178), Expect = 4.437e-14
Identity = 32/78 (41.03%), Postives = 51/78 (65.38%), Query Frame = 3
Query: 1515 YHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIR 1748
            + C  C K+F  +++LN H+R HTGE+P+ C  C K F+ S ++  H+R  H  E+P+ C +C +IF   +SL+RH+R
Sbjct:   93 FTCTQCGKSFSSSSHLNEHIRIHTGEKPFTCTQCGKSFNRSEHLNEHMRT-HTGEKPFTCIQCGKIFSISSSLNRHMR 169          

HSP 4 Score: 71.2478 bits (173), Expect = 2.364e-13
Identity = 33/79 (41.77%), Postives = 49/79 (62.03%), Query Frame = 3
Query: 1512 RYHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIR 1748
            R+ C  C K+F R   L  H+  HTGE+P+ C  C K FS SS++  H+R IH  E+P+ CT+C + F++   L+ H+R
Sbjct:   64 RFTCTQCGKSFGRKDILKIHMMIHTGEKPFTCTQCGKSFSSSSHLNEHIR-IHTGEKPFTCTQCGKSFNRSEHLNEHMR 141          

HSP 5 Score: 69.707 bits (169), Expect = 8.084e-13
Identity = 29/67 (43.28%), Postives = 47/67 (70.15%), Query Frame = 3
Query: 1515 YHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIF 1715
            + C  C K+F ++++L  H+R HTGE+P+ C  C K FS SS+++ H+R IH  E+P+ CT+C++ F
Sbjct:  177 FTCTQCGKSFSQSSSLKEHMRIHTGEKPFTCTQCGKSFSQSSSLKEHIR-IHTGEKPFTCTQCEKSF 242          

HSP 6 Score: 65.0846 bits (157), Expect = 2.806e-11
Identity = 38/112 (33.93%), Postives = 55/112 (49.11%), Query Frame = 3
Query:  576 CDVCNKPCRSQVSLIRHRKQHQNPNSHYSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYTQYSNLCRHKRMSRDCQRKAKCTECGMEFTNGKTYDTHKQHCLGLDP 911
            C  C K       L  H + H      ++C  CGK F ISS L +HM IH+  KP++C  C KS++Q S+L  H R+    ++   CT+CG  F+   +   H +   G  P
Sbjct:  123 CTQCGKSFNRSEHLNEHMRTHTGEKP-FTCIQCGKIFSISSSLNRHMRIHTGEKPFTCTQCGKSFSQSSSLKEHMRIHTG-EKPFTCTQCGKSFSQSSSLKEHIRIHTGEKP 232          

HSP 7 Score: 63.929 bits (154), Expect = 5.650e-11
Identity = 37/95 (38.95%), Postives = 52/95 (54.74%), Query Frame = 3
Query:  576 CDVCNKPCRSQVSLIRHRKQHQNPNSHYSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYTQYSNLCRHKRMSRDCQRKAKCTECGMEFT 860
            C  C K      SL RH + H      ++C  CGK+F  SS LK+HM IH+  KP++C  C KS++Q S+L  H R+    ++   CT+C   FT
Sbjct:  151 CIQCGKIFSISSSLNRHMRIHTGEKP-FTCTQCGKSFSQSSSLKEHMRIHTGEKPFTCTQCGKSFSQSSSLKEHIRIHTG-EKPFTCTQCEKSFT 243          

HSP 8 Score: 58.151 bits (139), Expect = 4.766e-9
Identity = 35/112 (31.25%), Postives = 54/112 (48.21%), Query Frame = 3
Query:  576 CDVCNKPCRSQVSLIRHRKQHQNPNSHYSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYTQYSNLCRHKRMSRDCQRKAKCTECGMEFTNGKTYDTHKQHCLGLDP 911
            C  C K   S   L  H + H      ++C  CGK+F  S  L +HM  H+  KP++C+ C K ++  S+L RH R+    ++   CT+CG  F+   +   H +   G  P
Sbjct:   95 CTQCGKSFSSSSHLNEHIRIHTGEKP-FTCTQCGKSFNRSEHLNEHMRTHTGEKPFTCIQCGKIFSISSSLNRHMRIHTG-EKPFTCTQCGKSFSQSSSLKEHMRIHTGEKP 204          

HSP 9 Score: 54.299 bits (129), Expect = 1.044e-7
Identity = 26/67 (38.81%), Postives = 38/67 (56.72%), Query Frame = 3
Query:  576 CDVCNKPCRSQVSLIRHRKQHQNPNSHYSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYTQ 776
            C  C K      SL  H + H      ++C  CGK+F  SS LK+H+ IH+  KP++C  C+KS+T+
Sbjct:  179 CTQCGKSFSQSSSLKEHMRIHTGEKP-FTCTQCGKSFSQSSSLKEHIRIHTGEKPFTCTQCEKSFTR 244          
BLAST of SMED30024013 vs. Ensembl Zebrafish
Match: BX005085.1 (pep chromosome:GRCz11:3:7123912:7129057:-1 gene:ENSDARG00000089947.3 transcript:ENSDART00000125947.3 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:BX005085.1)

HSP 1 Score: 86.2705 bits (212), Expect = 4.340e-18
Identity = 36/78 (46.15%), Postives = 60/78 (76.92%), Query Frame = 3
Query: 1515 YHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIR 1748
            + C  C K+F R++NLN+H+R+HTGE+P+ C  C K FS SSN+ +H++ IH +E+P++CT+C + F QL++L++H +
Sbjct:   84 FTCTQCGKSFGRSSNLNQHMRSHTGEKPFTCTLCGKSFSHSSNLNKHMK-IHPEEKPFRCTQCGKSFSQLSNLNKHTK 160          

HSP 2 Score: 78.5666 bits (192), Expect = 1.923e-15
Identity = 35/78 (44.87%), Postives = 55/78 (70.51%), Query Frame = 3
Query: 1515 YHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIR 1748
            Y C  C K+F   +NLN H++ H+GE+P+ C+ C K FS SSN+ +H++ IH +ERP+ CT+C + F   +S++RH+R
Sbjct:  224 YACTECGKSFIFLSNLNIHMKVHSGEKPFTCSQCGKSFSRSSNLNQHMK-IHTEERPFTCTQCGKCFSHSSSVNRHMR 300          

HSP 3 Score: 77.7962 bits (190), Expect = 3.413e-15
Identity = 36/78 (46.15%), Postives = 55/78 (70.51%), Query Frame = 3
Query: 1515 YHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIR 1748
            + C LC K+F  ++NLN+H++THTGE P  C  C K FS SSN+ +H++ IH  E+PY CT+C + F  L++L+ H++
Sbjct:  168 FTCTLCGKSFIFSSNLNKHMKTHTGENPLTCTQCGKSFSQSSNLNKHMK-IHTGEKPYACTECGKSFIFLSNLNIHMK 244          

HSP 4 Score: 72.7886 bits (177), Expect = 1.317e-13
Identity = 31/78 (39.74%), Postives = 51/78 (65.38%), Query Frame = 3
Query: 1515 YHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIR 1748
            + C  C K+F + +NLN+H + HTGE+P+ C  C K F  SSN+ +H++  H  E P  CT+C + F Q ++L++H++
Sbjct:  140 FRCTQCGKSFSQLSNLNKHTKIHTGEKPFTCTLCGKSFIFSSNLNKHMK-THTGENPLTCTQCGKSFSQSSNLNKHMK 216          

HSP 5 Score: 63.5438 bits (153), Expect = 1.581e-10
Identity = 37/105 (35.24%), Postives = 57/105 (54.29%), Query Frame = 3
Query:  570 LFCDVCNKPCRSQVSLIRHRKQHQNPNSHYSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYTQYSNLCRHKRMSRDCQRKAKCTECGMEFTNGKTYDTH 884
            L C  C K      +L +H K H      Y+C  CGK+F   S L  HM +HS  KP++C  C KS+++ SNL +H ++  + +R   CT+CG  F++  + + H
Sbjct:  196 LTCTQCGKSFSQSSNLNKHMKIHTGEKP-YACTECGKSFIFLSNLNIHMKVHSGEKPFTCSQCGKSFSRSSNLNQHMKIHTE-ERPFTCTQCGKCFSHSSSVNRH 298          

HSP 6 Score: 63.1586 bits (152), Expect = 2.154e-10
Identity = 29/76 (38.16%), Postives = 46/76 (60.53%), Query Frame = 3
Query: 1521 CHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIR 1748
            C  C  +     NL  H+R HTGE+P+ C  C K F  SSN+ +H+R+ H  E+P+ CT C + F   ++L++H++
Sbjct:   58 CTQCGTSCEGKGNLKIHMRIHTGEKPFTCTQCGKSFGRSSNLNQHMRS-HTGEKPFTCTLCGKSFSHSSNLNKHMK 132          

HSP 7 Score: 62.003 bits (149), Expect = 5.956e-10
Identity = 37/112 (33.04%), Postives = 56/112 (50.00%), Query Frame = 3
Query:  576 CDVCNKPCRSQVSLIRHRKQHQNPNSHYSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYTQYSNLCRHKRMSRDCQRKAKCTECGMEFTNGKTYDTHKQHCLGLDP 911
            C  C K      +L +H + H      ++C +CGK+F  SS L +HM IH   KP+ C  C KS++Q SNL +H ++    ++   CT CG  F      + H +   G +P
Sbjct:   86 CTQCGKSFGRSSNLNQHMRSHTGEKP-FTCTLCGKSFSHSSNLNKHMKIHPEEKPFRCTQCGKSFSQLSNLNKHTKIHTG-EKPFTCTLCGKSFIFSSNLNKHMKTHTGENP 195          

HSP 8 Score: 61.2326 bits (147), Expect = 9.206e-10
Identity = 56/206 (27.18%), Postives = 87/206 (42.23%), Query Frame = 3
Query:  273 SQFQCPDCGANFSSRGPLKRHQNALCKNPNGLLIKTRQTIFKHNSVIQRKQVLGTNQNCTTTNLSGGSEHRKTSSDYKQSVSFPISKLSSPISNGSNLN-----------LFCDVCNKPCRSQVSLIRHRKQHQNPNSHYSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYTQYSNLCRHKRMSRDCQRKAKCTECGMEF 857
            + F C  CG +   +G LK H          + I T +  F   +  Q  +  G + N         ++H ++ +  K    F  +      S+ SNLN             C  C K      +L +H K H      ++C +CGK+F  SS L +HM  H+   P +C  C KS++Q SNL +H ++    ++   CTECG  F
Sbjct:   54 THFICTQCGTSCEGKGNLKIH----------MRIHTGEKPF---TCTQCGKSFGRSSNL--------NQHMRSHTGEK---PFTCTLCGKSFSHSSNLNKHMKIHPEEKPFRCTQCGKSFSQLSNLNKHTKIHTGEKP-FTCTLCGKSFIFSSNLNKHMKTHTGENPLTCTQCGKSFSQSSNLNKHMKIHTG-EKPYACTECGKSF 233          

HSP 9 Score: 53.5286 bits (127), Expect = 3.379e-7
Identity = 35/112 (31.25%), Postives = 53/112 (47.32%), Query Frame = 3
Query:  576 CDVCNKPCRSQVSLIRHRKQHQNPNSHYSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYTQYSNLCRHKRMSRDCQRKAKCTECGMEFTNGKTYDTHKQHCLGLDP 911
            C +C K      +L +H K H      + C  CGK+F   S L +H  IH+  KP++C  C KS+   SNL +H + +   +    CT+CG  F+     + H +   G  P
Sbjct:  114 CTLCGKSFSHSSNLNKHMKIHPEEKP-FRCTQCGKSFSQLSNLNKHTKIHTGEKPFTCTLCGKSFIFSSNLNKHMK-THTGENPLTCTQCGKSFSQSSNLNKHMKIHTGEKP 223          

HSP 10 Score: 50.447 bits (119), Expect = 2.971e-6
Identity = 28/77 (36.36%), Postives = 41/77 (53.25%), Query Frame = 3
Query:  576 CDVCNKPCRSQVSLIRHRKQHQNPNSHYSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYTQYSNLCRHKRM 806
            C  C K      +L  H K H      ++C  CGK+F  SS L QHM IH+  +P++C  C K ++  S++ RH R+
Sbjct:  226 CTECGKSFIFLSNLNIHMKVHSGEKP-FTCSQCGKSFSRSSNLNQHMKIHTEERPFTCTQCGKCFSHSSSVNRHMRI 301          
BLAST of SMED30024013 vs. Ensembl Xenopus
Match: ENSXETT00000031589.1 (MDS1 and EVI1 complex locus protein EVI1-A-like [Source:NCBI gene;Acc:101731113])

HSP 1 Score: 130.568 bits (327), Expect = 3.606e-32
Identity = 56/80 (70.00%), Postives = 67/80 (83.75%), Query Frame = 3
Query: 1512 RYHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIRQ 1751
            RY C  C K FPR+ANL RHLRTHTGEQPY+C +CD+ FSISSN+QRHVRNIHNKE+P+KC  CDR F Q T+LDRH+++
Sbjct:  131 RYTCRYCGKIFPRSANLTRHLRTHTGEQPYRCKYCDRSFSISSNLQRHVRNIHNKEKPFKCHLCDRCFGQQTNLDRHLKK 210          

HSP 2 Score: 55.8398 bits (133), Expect = 1.276e-7
Identity = 24/56 (42.86%), Postives = 36/56 (64.29%), Query Frame = 3
Query: 1587 GEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIRQL 1754
            G++ Y C +C K F  S+N+ RH+R  H  E+PY+C  CDR F   ++L RH+R +
Sbjct:  128 GKERYTCRYCGKIFPRSANLTRHLRT-HTGEQPYRCKYCDRSFSISSNLQRHVRNI 182          

HSP 3 Score: 53.5286 bits (127), Expect = 7.247e-7
Identity = 29/80 (36.25%), Postives = 42/80 (52.50%), Query Frame = 3
Query:  657 YSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYTQYSNLCRHKRMSRDCQRKAKCTECGMEFTNGKTYDTH-KQH 893
            Y+C  CGK F  S+ L +H+  H+  +PY C  C +S++  SNL RH R   + ++  KC  C   F      D H K+H
Sbjct:  132 YTCRYCGKIFPRSANLTRHLRTHTGEQPYRCKYCDRSFSISSNLQRHVRNIHNKEKPFKCHLCDRCFGQQTNLDRHLKKH 211          
BLAST of SMED30024013 vs. Ensembl Xenopus
Match: PRDM16 (PR domain 16 [Source:NCBI gene;Acc:100485690])

HSP 1 Score: 128.642 bits (322), Expect = 2.693e-30
Identity = 55/80 (68.75%), Postives = 67/80 (83.75%), Query Frame = 3
Query: 1512 RYHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIRQ 1751
            RY C  C K FPR+ANL RHLRTHTGEQPY+C +CD+ FSISSN+QRHVRNIHNKE+P+KC  C+R F Q T+LDRH+++
Sbjct:  842 RYTCRYCGKIFPRSANLTRHLRTHTGEQPYRCKYCDRSFSISSNLQRHVRNIHNKEKPFKCHLCNRCFGQQTNLDRHLKK 921          

HSP 2 Score: 114.775 bits (286), Expect = 7.776e-26
Identity = 76/216 (35.19%), Postives = 114/216 (52.78%), Query Frame = 3
Query:  279 FQCPDCGANFSSRGPLKRHQNALCKNPN----GLLIKTRQTIFKHNSVIQRK--QVLGTNQNCTTTNLSGGSEHRKTSSDY-KQSVSFPISKLSSPISNGSNLNLFCDVCNKPCRSQVSLIRH-RKQHQNPNSHYSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYTQYSNLCRHKRMSRDCQRKAKCTECGMEFTNGKTYDTHKQHCLG 902
            F+C +C   F S+  L+RHQ   C   N     L  + +Q  F +  + + K  +    N+     ++   +E R+   D   ++ ++  + +   +S+ S     C+ C K      +L RH R QH    +H +C  CGKTF  SSGLKQH HIHS+ KP+ C  C KSYTQ+SNLCRHKRM  DC+ + KC +CG  F+   + + H++ C G
Sbjct:  127 FRCEECDELFQSKLDLRRHQKYACTTVNHIYESLNEEIKQEGFDNEQMHECKDCERPFPNKYSLEQHMIIHTEEREYKCDQCPKAFNWKSNLIRHQMSHDSGKRFECENCVKVFTDPSNLQRHIRSQHVGARAH-ACPDCGKTFATSSGLKQHKHIHSTVKPFICEVCHKSYTQFSNLCRHKRMHADCRTQIKCKDCGQMFSTTSSLNKHRRFCEG 341          

HSP 3 Score: 60.8474 bits (146), Expect = 4.961e-9
Identity = 29/80 (36.25%), Postives = 49/80 (61.25%), Query Frame = 3
Query: 1512 RYHCHLCLKTFPRAANLNRHLRT-HTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIR 1748
            R+ C  C+K F   +NL RH+R+ H G + + C  C K F+ SS +++H ++IH+  +P+ C  C + + Q ++L RH R
Sbjct:  230 RFECENCVKVFTDPSNLQRHIRSQHVGARAHACPDCGKTFATSSGLKQH-KHIHSTVKPFICEVCHKSYTQFSNLCRHKR 308          

HSP 4 Score: 58.151 bits (139), Expect = 4.014e-8
Identity = 28/78 (35.90%), Postives = 45/78 (57.69%), Query Frame = 3
Query: 1515 YHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIR 1748
            + C  C + FP   +L +H+  HT E+ YKC  C K F+  SN+ RH  + H+  + ++C  C ++F   ++L RHIR
Sbjct:  175 HECKDCERPFPNKYSLEQHMIIHTEEREYKCDQCPKAFNWKSNLIRHQMS-HDSGKRFECENCVKVFTDPSNLQRHIR 251          

HSP 5 Score: 55.8398 bits (133), Expect = 2.008e-7
Identity = 24/56 (42.86%), Postives = 36/56 (64.29%), Query Frame = 3
Query: 1587 GEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIRQL 1754
            G++ Y C +C K F  S+N+ RH+R  H  E+PY+C  CDR F   ++L RH+R +
Sbjct:  839 GKERYTCRYCGKIFPRSANLTRHLR-THTGEQPYRCKYCDRSFSISSNLQRHVRNI 893          

HSP 6 Score: 55.4546 bits (132), Expect = 2.342e-7
Identity = 27/77 (35.06%), Postives = 40/77 (51.95%), Query Frame = 3
Query: 1512 RYHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRH 1742
             Y C  C K F   +NL RH  +H   + ++C  C K F+  SN+QRH+R+ H   R + C  C + F   + L +H
Sbjct:  202 EYKCDQCPKAFNWKSNLIRHQMSHDSGKRFECENCVKVFTDPSNLQRHIRSQHVGARAHACPDCGKTFATSSGLKQH 278          

HSP 7 Score: 54.299 bits (129), Expect = 6.321e-7
Identity = 29/80 (36.25%), Postives = 42/80 (52.50%), Query Frame = 3
Query:  657 YSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYTQYSNLCRHKRMSRDCQRKAKCTECGMEFTNGKTYDTH-KQH 893
            Y+C  CGK F  S+ L +H+  H+  +PY C  C +S++  SNL RH R   + ++  KC  C   F      D H K+H
Sbjct:  843 YTCRYCGKIFPRSANLTRHLRTHTGEQPYRCKYCDRSFSISSNLQRHVRNIHNKEKPFKCHLCNRCFGQQTNLDRHLKKH 922          

HSP 8 Score: 50.8322 bits (120), Expect = 8.017e-6
Identity = 31/93 (33.33%), Postives = 45/93 (48.39%), Query Frame = 3
Query:  522 PISKLSSPISNGSNLNLFCDVCNKPCRSQVSLIRHRKQHQNPNSHYSCDVCGKTFKISSGLKQHM-HIHSSNKPYSCLNCQKSYTQYSNLCRH 797
            P + LS  I         C  C K      +L RH + H      Y C  C ++F ISS L++H+ +IH+  KP+ C  C + + Q +NL RH
Sbjct:  827 PPTTLSDSILRKGKERYTCRYCGKIFPRSANLTRHLRTHTGEQP-YRCKYCDRSFSISSNLQRHVRNIHNKEKPFKCHLCNRCFGQQTNLDRH 918          
BLAST of SMED30024013 vs. Ensembl Xenopus
Match: PRDM16 (PR domain 16 [Source:NCBI gene;Acc:100485690])

HSP 1 Score: 128.257 bits (321), Expect = 3.299e-30
Identity = 55/80 (68.75%), Postives = 67/80 (83.75%), Query Frame = 3
Query: 1512 RYHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIRQ 1751
            RY C  C K FPR+ANL RHLRTHTGEQPY+C +CD+ FSISSN+QRHVRNIHNKE+P+KC  C+R F Q T+LDRH+++
Sbjct:  815 RYTCRYCGKIFPRSANLTRHLRTHTGEQPYRCKYCDRSFSISSNLQRHVRNIHNKEKPFKCHLCNRCFGQQTNLDRHLKK 894          

HSP 2 Score: 114.39 bits (285), Expect = 1.017e-25
Identity = 76/216 (35.19%), Postives = 114/216 (52.78%), Query Frame = 3
Query:  279 FQCPDCGANFSSRGPLKRHQNALCKNPN----GLLIKTRQTIFKHNSVIQRK--QVLGTNQNCTTTNLSGGSEHRKTSSDY-KQSVSFPISKLSSPISNGSNLNLFCDVCNKPCRSQVSLIRH-RKQHQNPNSHYSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYTQYSNLCRHKRMSRDCQRKAKCTECGMEFTNGKTYDTHKQHCLG 902
            F+C +C   F S+  L+RHQ   C   N     L  + +Q  F +  + + K  +    N+     ++   +E R+   D   ++ ++  + +   +S+ S     C+ C K      +L RH R QH    +H +C  CGKTF  SSGLKQH HIHS+ KP+ C  C KSYTQ+SNLCRHKRM  DC+ + KC +CG  F+   + + H++ C G
Sbjct:  101 FRCEECDELFQSKLDLRRHQKYACTTVNHIYESLNEEIKQEGFDNEQMHECKDCERPFPNKYSLEQHMIIHTEEREYKCDQCPKAFNWKSNLIRHQMSHDSGKRFECENCVKVFTDPSNLQRHIRSQHVGARAH-ACPDCGKTFATSSGLKQHKHIHSTVKPFICEVCHKSYTQFSNLCRHKRMHADCRTQIKCKDCGQMFSTTSSLNKHRRFCEG 315          

HSP 3 Score: 60.8474 bits (146), Expect = 5.667e-9
Identity = 29/80 (36.25%), Postives = 49/80 (61.25%), Query Frame = 3
Query: 1512 RYHCHLCLKTFPRAANLNRHLRT-HTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIR 1748
            R+ C  C+K F   +NL RH+R+ H G + + C  C K F+ SS +++H ++IH+  +P+ C  C + + Q ++L RH R
Sbjct:  204 RFECENCVKVFTDPSNLQRHIRSQHVGARAHACPDCGKTFATSSGLKQH-KHIHSTVKPFICEVCHKSYTQFSNLCRHKR 282          

HSP 4 Score: 57.7658 bits (138), Expect = 5.006e-8
Identity = 28/78 (35.90%), Postives = 45/78 (57.69%), Query Frame = 3
Query: 1515 YHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIR 1748
            + C  C + FP   +L +H+  HT E+ YKC  C K F+  SN+ RH  + H+  + ++C  C ++F   ++L RHIR
Sbjct:  149 HECKDCERPFPNKYSLEQHMIIHTEEREYKCDQCPKAFNWKSNLIRHQMS-HDSGKRFECENCVKVFTDPSNLQRHIR 225          

HSP 5 Score: 55.8398 bits (133), Expect = 1.939e-7
Identity = 24/56 (42.86%), Postives = 36/56 (64.29%), Query Frame = 3
Query: 1587 GEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIRQL 1754
            G++ Y C +C K F  S+N+ RH+R  H  E+PY+C  CDR F   ++L RH+R +
Sbjct:  812 GKERYTCRYCGKIFPRSANLTRHLR-THTGEQPYRCKYCDRSFSISSNLQRHVRNI 866          

HSP 6 Score: 55.4546 bits (132), Expect = 2.900e-7
Identity = 27/77 (35.06%), Postives = 40/77 (51.95%), Query Frame = 3
Query: 1512 RYHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRH 1742
             Y C  C K F   +NL RH  +H   + ++C  C K F+  SN+QRH+R+ H   R + C  C + F   + L +H
Sbjct:  176 EYKCDQCPKAFNWKSNLIRHQMSHDSGKRFECENCVKVFTDPSNLQRHIRSQHVGARAHACPDCGKTFATSSGLKQH 252          

HSP 7 Score: 53.9138 bits (128), Expect = 7.378e-7
Identity = 27/76 (35.53%), Postives = 39/76 (51.32%), Query Frame = 3
Query:  657 YSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYTQYSNLCRHKRMSRDCQRKAKCTECGMEFTNGKTYDTH 884
            Y+C  CGK F  S+ L +H+  H+  +PY C  C +S++  SNL RH R   + ++  KC  C   F      D H
Sbjct:  816 YTCRYCGKIFPRSANLTRHLRTHTGEQPYRCKYCDRSFSISSNLQRHVRNIHNKEKPFKCHLCNRCFGQQTNLDRH 891          

HSP 8 Score: 50.447 bits (119), Expect = 9.540e-6
Identity = 31/93 (33.33%), Postives = 45/93 (48.39%), Query Frame = 3
Query:  522 PISKLSSPISNGSNLNLFCDVCNKPCRSQVSLIRHRKQHQNPNSHYSCDVCGKTFKISSGLKQHM-HIHSSNKPYSCLNCQKSYTQYSNLCRH 797
            P + LS  I         C  C K      +L RH + H      Y C  C ++F ISS L++H+ +IH+  KP+ C  C + + Q +NL RH
Sbjct:  800 PPTTLSDSILRKGKERYTCRYCGKIFPRSANLTRHLRTHTGEQP-YRCKYCDRSFSISSNLQRHVRNIHNKEKPFKCHLCNRCFGQQTNLDRH 891          
BLAST of SMED30024013 vs. Ensembl Xenopus
Match: PRDM16 (PR domain 16 [Source:NCBI gene;Acc:100485690])

HSP 1 Score: 128.257 bits (321), Expect = 3.621e-30
Identity = 55/80 (68.75%), Postives = 67/80 (83.75%), Query Frame = 3
Query: 1512 RYHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIRQ 1751
            RY C  C K FPR+ANL RHLRTHTGEQPY+C +CD+ FSISSN+QRHVRNIHNKE+P+KC  C+R F Q T+LDRH+++
Sbjct:  842 RYTCRYCGKIFPRSANLTRHLRTHTGEQPYRCKYCDRSFSISSNLQRHVRNIHNKEKPFKCHLCNRCFGQQTNLDRHLKK 921          

HSP 2 Score: 114.775 bits (286), Expect = 8.069e-26
Identity = 76/216 (35.19%), Postives = 114/216 (52.78%), Query Frame = 3
Query:  279 FQCPDCGANFSSRGPLKRHQNALCKNPN----GLLIKTRQTIFKHNSVIQRK--QVLGTNQNCTTTNLSGGSEHRKTSSDY-KQSVSFPISKLSSPISNGSNLNLFCDVCNKPCRSQVSLIRH-RKQHQNPNSHYSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYTQYSNLCRHKRMSRDCQRKAKCTECGMEFTNGKTYDTHKQHCLG 902
            F+C +C   F S+  L+RHQ   C   N     L  + +Q  F +  + + K  +    N+     ++   +E R+   D   ++ ++  + +   +S+ S     C+ C K      +L RH R QH    +H +C  CGKTF  SSGLKQH HIHS+ KP+ C  C KSYTQ+SNLCRHKRM  DC+ + KC +CG  F+   + + H++ C G
Sbjct:  127 FRCEECDELFQSKLDLRRHQKYACTTVNHIYESLNEEIKQEGFDNEQMHECKDCERPFPNKYSLEQHMIIHTEEREYKCDQCPKAFNWKSNLIRHQMSHDSGKRFECENCVKVFTDPSNLQRHIRSQHVGARAH-ACPDCGKTFATSSGLKQHKHIHSTVKPFICEVCHKSYTQFSNLCRHKRMHADCRTQIKCKDCGQMFSTTSSLNKHRRFCEG 341          

HSP 3 Score: 60.8474 bits (146), Expect = 5.920e-9
Identity = 29/80 (36.25%), Postives = 49/80 (61.25%), Query Frame = 3
Query: 1512 RYHCHLCLKTFPRAANLNRHLRT-HTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIR 1748
            R+ C  C+K F   +NL RH+R+ H G + + C  C K F+ SS +++H ++IH+  +P+ C  C + + Q ++L RH R
Sbjct:  230 RFECENCVKVFTDPSNLQRHIRSQHVGARAHACPDCGKTFATSSGLKQH-KHIHSTVKPFICEVCHKSYTQFSNLCRHKR 308          

HSP 4 Score: 57.7658 bits (138), Expect = 4.629e-8
Identity = 28/78 (35.90%), Postives = 45/78 (57.69%), Query Frame = 3
Query: 1515 YHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIR 1748
            + C  C + FP   +L +H+  HT E+ YKC  C K F+  SN+ RH  + H+  + ++C  C ++F   ++L RHIR
Sbjct:  175 HECKDCERPFPNKYSLEQHMIIHTEEREYKCDQCPKAFNWKSNLIRHQMS-HDSGKRFECENCVKVFTDPSNLQRHIR 251          

HSP 5 Score: 55.4546 bits (132), Expect = 2.544e-7
Identity = 24/56 (42.86%), Postives = 36/56 (64.29%), Query Frame = 3
Query: 1587 GEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIRQL 1754
            G++ Y C +C K F  S+N+ RH+R  H  E+PY+C  CDR F   ++L RH+R +
Sbjct:  839 GKERYTCRYCGKIFPRSANLTRHLR-THTGEQPYRCKYCDRSFSISSNLQRHVRNI 893          

HSP 6 Score: 55.4546 bits (132), Expect = 2.796e-7
Identity = 27/77 (35.06%), Postives = 40/77 (51.95%), Query Frame = 3
Query: 1512 RYHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRH 1742
             Y C  C K F   +NL RH  +H   + ++C  C K F+  SN+QRH+R+ H   R + C  C + F   + L +H
Sbjct:  202 EYKCDQCPKAFNWKSNLIRHQMSHDSGKRFECENCVKVFTDPSNLQRHIRSQHVGARAHACPDCGKTFATSSGLKQH 278          

HSP 7 Score: 54.299 bits (129), Expect = 6.250e-7
Identity = 29/80 (36.25%), Postives = 42/80 (52.50%), Query Frame = 3
Query:  657 YSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYTQYSNLCRHKRMSRDCQRKAKCTECGMEFTNGKTYDTH-KQH 893
            Y+C  CGK F  S+ L +H+  H+  +PY C  C +S++  SNL RH R   + ++  KC  C   F      D H K+H
Sbjct:  843 YTCRYCGKIFPRSANLTRHLRTHTGEQPYRCKYCDRSFSISSNLQRHVRNIHNKEKPFKCHLCNRCFGQQTNLDRHLKKH 922          

HSP 8 Score: 50.8322 bits (120), Expect = 7.861e-6
Identity = 31/93 (33.33%), Postives = 45/93 (48.39%), Query Frame = 3
Query:  522 PISKLSSPISNGSNLNLFCDVCNKPCRSQVSLIRHRKQHQNPNSHYSCDVCGKTFKISSGLKQHM-HIHSSNKPYSCLNCQKSYTQYSNLCRH 797
            P + LS  I         C  C K      +L RH + H      Y C  C ++F ISS L++H+ +IH+  KP+ C  C + + Q +NL RH
Sbjct:  827 PPTTLSDSILRKGKERYTCRYCGKIFPRSANLTRHLRTHTGEQP-YRCKYCDRSFSISSNLQRHVRNIHNKEKPFKCHLCNRCFGQQTNLDRH 918          
BLAST of SMED30024013 vs. Ensembl Xenopus
Match: cip2a (cell proliferation regulating inhibitor of protein phosphatase 2A [Source:Xenbase;Acc:XB-GENE-5926363])

HSP 1 Score: 86.2705 bits (212), Expect = 1.707e-17
Identity = 42/79 (53.16%), Postives = 51/79 (64.56%), Query Frame = 3
Query: 1512 RYHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIR 1748
            RY CH+C K+F   +NL  H R HTGE+PYKC  CD CFS  S++ RH R  H+  RPYKC +CD+ F Q   L  HIR
Sbjct:  189 RYKCHVCEKSFSENSNLIVHQRIHTGEKPYKCPECDICFSQHSSLVRHRRK-HSGARPYKCEECDKTFSQKGHLSNHIR 266          

HSP 2 Score: 75.485 bits (184), Expect = 6.865e-14
Identity = 35/76 (46.05%), Postives = 49/76 (64.47%), Query Frame = 3
Query: 1515 YHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRH 1742
            Y C  C KTF +  +L+ H+RTHTGE+PYKC  C KCFS  S++  H + IH  E+PY C  C + F ++++L  H
Sbjct:  246 YKCEECDKTFSQKGHLSNHIRTHTGERPYKCGECGKCFSEHSHLTGH-QKIHTGEKPYTCDVCHKSFSKISNLKAH 320          

HSP 3 Score: 72.0182 bits (175), Expect = 7.538e-13
Identity = 41/107 (38.32%), Postives = 53/107 (49.53%), Query Frame = 3
Query: 1512 RYHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVR---------------------------NIHNKERPYKCTKCDRIFDQLTSLDRHIRQ 1751
            RY C  C K F R ++L  H R HTGE+P+KC  CDK FS +S++  H+R                            IH  E+PYKC +CD  F Q +SL RH R+
Sbjct:  133 RYRCSECGKGFTRNSHLKAHRRIHTGERPFKCGECDKTFSENSHLTVHLRVHSGEKRYKCHVCEKSFSENSNLIVHQRIHTGEKPYKCPECDICFSQHSSLVRHRRK 239          

HSP 4 Score: 63.1586 bits (152), Expect = 5.690e-10
Identity = 33/77 (42.86%), Postives = 42/77 (54.55%), Query Frame = 3
Query:  576 CDVCNKPCRSQVSLIRHRKQHQNPNSHYSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYTQYSNLCRHKRM 806
            C  C K       L  H+K H      Y+CDVC K+F   S LK H  IH+  +PY+C  C KS+TQ+S L RH R+
Sbjct:  276 CGECGKCFSEHSHLTGHQKIHTGEKP-YTCDVCHKSFSKISNLKAHQQIHTGYRPYACTQCGKSFTQHSTLVRHHRV 351          

HSP 5 Score: 60.077 bits (144), Expect = 5.955e-9
Identity = 37/103 (35.92%), Postives = 50/103 (48.54%), Query Frame = 3
Query:  576 CDVCNKPCRSQVSLIRHRKQHQNPNSHYSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYTQYSNLCRHKRMSRDCQRKAKCTECGMEFTNGKTYDTH 884
            C+ C+K    +  L  H + H      Y C  CGK F   S L  H  IH+  KPY+C  C KS+++ SNL  H+++     R   CT+CG  FT   T   H
Sbjct:  248 CEECDKTFSQKGHLSNHIRTHTGERP-YKCGECGKCFSEHSHLTGHQKIHTGEKPYTCDVCHKSFSKISNLKAHQQIHTG-YRPYACTQCGKSFTQHSTLVRH 348          

HSP 6 Score: 57.7658 bits (138), Expect = 2.983e-8
Identity = 33/112 (29.46%), Postives = 53/112 (47.32%), Query Frame = 3
Query:  576 CDVCNKPCRSQVSLIRHRKQHQNPNSHYSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYTQYSNLCRHKRMSRDCQRKAKCTECGMEFTNGKTYDTHKQHCLGLDP 911
            CD+C        SL+RHR++H      Y C+ C KTF     L  H+  H+  +PY C  C K ++++S+L  H+++    ++   C  C   F+       H+Q   G  P
Sbjct:  223 CDICFS---QHSSLVRHRRKHSGARP-YKCEECDKTFSQKGHLSNHIRTHTGERPYKCGECGKCFSEHSHLTGHQKIHTG-EKPYTCDVCHKSFSKISNLKAHQQIHTGYRP 329          

HSP 7 Score: 56.9954 bits (136), Expect = 4.882e-8
Identity = 34/95 (35.79%), Postives = 45/95 (47.37%), Query Frame = 3
Query:  576 CDVCNKPCRSQVSLIRHRKQHQNPNSHYSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYTQYSNLCRHKRMSRDCQRKAKCTECGMEFT 860
            C  C+K       L  H + H      Y C VC K+F  +S L  H  IH+  KPY C  C   ++Q+S+L RH+R      R  KC EC   F+
Sbjct:  164 CGECDKTFSENSHLTVHLRVHSG-EKRYKCHVCEKSFSENSNLIVHQRIHTGEKPYKCPECDICFSQHSSLVRHRR-KHSGARPYKCEECDKTFS 256          

HSP 8 Score: 55.8398 bits (133), Expect = 1.086e-7
Identity = 36/112 (32.14%), Postives = 54/112 (48.21%), Query Frame = 3
Query:  576 CDVCNKPCRSQVSLIRHRKQHQNPNSHYSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYTQYSNLCRHKRMSRDCQRKAKCTECGMEFTNGKTYDTHKQHCLGLDP 911
            C  C K       L  HR+ H      + C  C KTF  +S L  H+ +HS  K Y C  C+KS+++ SNL  H+R+    ++  KC EC + F+   +   H++   G  P
Sbjct:  136 CSECGKGFTRNSHLKAHRRIHTGERP-FKCGECDKTFSENSHLTVHLRVHSGEKRYKCHVCEKSFSENSNLIVHQRIHTG-EKPYKCPECDICFSQHSSLVRHRRKHSGARP 245          

HSP 9 Score: 53.1434 bits (126), Expect = 8.026e-7
Identity = 36/112 (32.14%), Postives = 50/112 (44.64%), Query Frame = 3
Query:  576 CDVCNKPCRSQVSLIRHRKQHQNPNSHYSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYTQYSNLCRHKRMSRDCQRKAKCTECGMEFTNGKTYDTHKQHCLGLDP 911
            C VC K      +LI H++ H      Y C  C   F   S L +H   HS  +PY C  C K+++Q  +L  H R +   +R  KC ECG  F+       H++   G  P
Sbjct:  192 CHVCEKSFSENSNLIVHQRIHTGEKP-YKCPECDICFSQHSSLVRHRRKHSGARPYKCEECDKTFSQKGHLSNHIR-THTGERPYKCGECGKCFSEHSHLTGHQKIHTGEKP 301          

HSP 10 Score: 51.9878 bits (123), Expect = 2.013e-6
Identity = 23/54 (42.59%), Postives = 37/54 (68.52%), Query Frame = 3
Query: 1587 GEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIR 1748
            GE+ Y+C+ C K F+ +S+++ H R IH  ERP+KC +CD+ F + + L  H+R
Sbjct:  130 GEKRYRCSECGKGFTRNSHLKAH-RRIHTGERPFKCGECDKTFSENSHLTVHLR 182          

HSP 11 Score: 49.6766 bits (117), Expect = 9.305e-6
Identity = 27/85 (31.76%), Postives = 43/85 (50.59%), Query Frame = 3
Query:  657 YSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYTQYSNLCRHKRMSRDCQRKAKCTECGMEFTNGKTYDTHKQHCLGLDP 911
            Y C  CGK F  +S LK H  IH+  +P+ C  C K++++ S+L  H R+    +++ KC  C   F+       H++   G  P
Sbjct:  134 YRCSECGKGFTRNSHLKAHRRIHTGERPFKCGECDKTFSENSHLTVHLRVHSG-EKRYKCHVCEKSFSENSNLIVHQRIHTGEKP 217          
BLAST of SMED30024013 vs. Ensembl Mouse
Match: Mecom (MDS1 and EVI1 complex locus [Source:MGI Symbol;Acc:MGI:95457])

HSP 1 Score: 131.339 bits (329), Expect = 1.540e-31
Identity = 56/80 (70.00%), Postives = 67/80 (83.75%), Query Frame = 3
Query: 1512 RYHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIRQ 1751
            RY C  C K FPR+ANL RHLRTHTGEQPY+C +CD+ FSISSN+QRHVRNIHNKE+P+KC  CDR F Q T+LDRH+++
Sbjct:  399 RYTCRYCGKIFPRSANLTRHLRTHTGEQPYRCKYCDRSFSISSNLQRHVRNIHNKEKPFKCHLCDRCFGQQTNLDRHLKK 478          

HSP 2 Score: 62.003 bits (149), Expect = 1.804e-9
Identity = 48/170 (28.24%), Postives = 78/170 (45.88%), Query Frame = 3
Query:  276 QFQCPDCGANFSSRGPLKRHQNALCKNPNGLLIKTRQTIFKHNSVIQRKQVLGTNQNCTT-----TNLSGGSEHRKTSSDYKQ--------SVSFPISKLSSPISNGSNLNLFCDVCNKPCRSQVSLIRH-RKQHQNPNSHYSCDVCGKTFKISSGLKQHMHIHSSNKPY 743
            Q +C DC   F S+  L  HQ   C  P+       + + ++       + +  NQ+C        +L    +H  + ++ ++        + ++  + +   +S+ S  +  C+ C K      +L RH R QH    +H +C  CGKTF  SSGLKQH HIHSS KP+
Sbjct:   20 QHRCEDCDQLFESKAELADHQKFPCSTPHSAFSMVEEDLQQNLESESDLREIHGNQDCKECDRVFPDLQSLEKHMLSHTEEREYKCDQCPKAFNWKSNLIRHQMSHDSGKHYECENCAKVFTDPSNLQRHIRSQHVGARAH-ACPECGKTFATSSGLKQHKHIHSSVKPF 188          

HSP 3 Score: 60.8474 bits (146), Expect = 3.661e-9
Identity = 29/76 (38.16%), Postives = 45/76 (59.21%), Query Frame = 3
Query: 1521 CHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIR 1748
            C  C + FP   +L +H+ +HT E+ YKC  C K F+  SN+ RH  + H+  + Y+C  C ++F   ++L RHIR
Sbjct:   77 CKECDRVFPDLQSLEKHMLSHTEEREYKCDQCPKAFNWKSNLIRHQMS-HDSGKHYECENCAKVFTDPSNLQRHIR 151          

HSP 4 Score: 57.3806 bits (137), Expect = 4.497e-8
Identity = 28/76 (36.84%), Postives = 41/76 (53.95%), Query Frame = 3
Query: 1515 YHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRH 1742
            Y C  C K F   +NL RH  +H   + Y+C  C K F+  SN+QRH+R+ H   R + C +C + F   + L +H
Sbjct:  103 YKCDQCPKAFNWKSNLIRHQMSHDSGKHYECENCAKVFTDPSNLQRHIRSQHVGARAHACPECGKTFATSSGLKQH 178          

HSP 5 Score: 55.4546 bits (132), Expect = 1.944e-7
Identity = 24/56 (42.86%), Postives = 36/56 (64.29%), Query Frame = 3
Query: 1587 GEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIRQL 1754
            G++ Y C +C K F  S+N+ RH+R  H  E+PY+C  CDR F   ++L RH+R +
Sbjct:  396 GKERYTCRYCGKIFPRSANLTRHLR-THTGEQPYRCKYCDRSFSISSNLQRHVRNI 450          

HSP 6 Score: 53.1434 bits (126), Expect = 8.396e-7
Identity = 30/83 (36.14%), Postives = 43/83 (51.81%), Query Frame = 3
Query:  657 YSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYTQYSNLCRHKRMSRDCQRKAKCTECGMEFTNGKTYDTH-KQHCLG 902
            Y+C  CGK F  S+ L +H+  H+  +PY C  C +S++  SNL RH R   + ++  KC  C   F      D H K+H  G
Sbjct:  400 YTCRYCGKIFPRSANLTRHLRTHTGEQPYRCKYCDRSFSISSNLQRHVRNIHNKEKPFKCHLCDRCFGQQTNLDRHLKKHENG 482          
BLAST of SMED30024013 vs. Ensembl Mouse
Match: Mecom (MDS1 and EVI1 complex locus [Source:MGI Symbol;Acc:MGI:95457])

HSP 1 Score: 130.568 bits (327), Expect = 1.564e-31
Identity = 56/80 (70.00%), Postives = 67/80 (83.75%), Query Frame = 3
Query: 1512 RYHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIRQ 1751
            RY C  C K FPR+ANL RHLRTHTGEQPY+C +CD+ FSISSN+QRHVRNIHNKE+P+KC  CDR F Q T+LDRH+++
Sbjct:  389 RYTCRYCGKIFPRSANLTRHLRTHTGEQPYRCKYCDRSFSISSNLQRHVRNIHNKEKPFKCHLCDRCFGQQTNLDRHLKK 468          

HSP 2 Score: 61.6178 bits (148), Expect = 2.034e-9
Identity = 48/170 (28.24%), Postives = 78/170 (45.88%), Query Frame = 3
Query:  276 QFQCPDCGANFSSRGPLKRHQNALCKNPNGLLIKTRQTIFKHNSVIQRKQVLGTNQNCTT-----TNLSGGSEHRKTSSDYKQ--------SVSFPISKLSSPISNGSNLNLFCDVCNKPCRSQVSLIRH-RKQHQNPNSHYSCDVCGKTFKISSGLKQHMHIHSSNKPY 743
            Q +C DC   F S+  L  HQ   C  P+       + + ++       + +  NQ+C        +L    +H  + ++ ++        + ++  + +   +S+ S  +  C+ C K      +L RH R QH    +H +C  CGKTF  SSGLKQH HIHSS KP+
Sbjct:   10 QHRCEDCDQLFESKAELADHQKFPCSTPHSAFSMVEEDLQQNLESESDLREIHGNQDCKECDRVFPDLQSLEKHMLSHTEEREYKCDQCPKAFNWKSNLIRHQMSHDSGKHYECENCAKVFTDPSNLQRHIRSQHVGARAH-ACPECGKTFATSSGLKQHKHIHSSVKPF 178          

HSP 3 Score: 60.4622 bits (145), Expect = 4.245e-9
Identity = 29/76 (38.16%), Postives = 45/76 (59.21%), Query Frame = 3
Query: 1521 CHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIR 1748
            C  C + FP   +L +H+ +HT E+ YKC  C K F+  SN+ RH  + H+  + Y+C  C ++F   ++L RHIR
Sbjct:   67 CKECDRVFPDLQSLEKHMLSHTEEREYKCDQCPKAFNWKSNLIRHQMS-HDSGKHYECENCAKVFTDPSNLQRHIR 141          

HSP 4 Score: 56.9954 bits (136), Expect = 4.703e-8
Identity = 28/76 (36.84%), Postives = 41/76 (53.95%), Query Frame = 3
Query: 1515 YHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRH 1742
            Y C  C K F   +NL RH  +H   + Y+C  C K F+  SN+QRH+R+ H   R + C +C + F   + L +H
Sbjct:   93 YKCDQCPKAFNWKSNLIRHQMSHDSGKHYECENCAKVFTDPSNLQRHIRSQHVGARAHACPECGKTFATSSGLKQH 168          

HSP 5 Score: 55.0694 bits (131), Expect = 2.209e-7
Identity = 24/56 (42.86%), Postives = 36/56 (64.29%), Query Frame = 3
Query: 1587 GEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIRQL 1754
            G++ Y C +C K F  S+N+ RH+R  H  E+PY+C  CDR F   ++L RH+R +
Sbjct:  386 GKERYTCRYCGKIFPRSANLTRHLR-THTGEQPYRCKYCDRSFSISSNLQRHVRNI 440          

HSP 6 Score: 53.5286 bits (127), Expect = 6.728e-7
Identity = 27/76 (35.53%), Postives = 39/76 (51.32%), Query Frame = 3
Query:  657 YSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYTQYSNLCRHKRMSRDCQRKAKCTECGMEFTNGKTYDTH 884
            Y+C  CGK F  S+ L +H+  H+  +PY C  C +S++  SNL RH R   + ++  KC  C   F      D H
Sbjct:  390 YTCRYCGKIFPRSANLTRHLRTHTGEQPYRCKYCDRSFSISSNLQRHVRNIHNKEKPFKCHLCDRCFGQQTNLDRH 465          
BLAST of SMED30024013 vs. Ensembl Mouse
Match: Mecom (MDS1 and EVI1 complex locus [Source:MGI Symbol;Acc:MGI:95457])

HSP 1 Score: 131.724 bits (330), Expect = 1.602e-31
Identity = 56/80 (70.00%), Postives = 67/80 (83.75%), Query Frame = 3
Query: 1512 RYHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIRQ 1751
            RY C  C K FPR+ANL RHLRTHTGEQPY+C +CD+ FSISSN+QRHVRNIHNKE+P+KC  CDR F Q T+LDRH+++
Sbjct:  723 RYTCRYCGKIFPRSANLTRHLRTHTGEQPYRCKYCDRSFSISSNLQRHVRNIHNKEKPFKCHLCDRCFGQQTNLDRHLKK 802          

HSP 2 Score: 117.472 bits (293), Expect = 6.197e-27
Identity = 74/223 (33.18%), Postives = 113/223 (50.67%), Query Frame = 3
Query:  276 QFQCPDCGANFSSRGPLKRHQNALCKNPNGLLIKTRQTIFKHNSVIQRKQVLGTNQNCTT-----TNLSGGSEHRKTSSDYKQ--------SVSFPISKLSSPISNGSNLNLFCDVCNKPCRSQVSLIRH-RKQHQNPNSHYSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYTQYSNLCRHKRMSRDCQRKAKCTECGMEFTNGKTYDTHKQHCLG 902
            Q +C DC   F S+  L  HQ   C  P+       + + ++       + +  NQ+C        +L    +H  + ++ ++        + ++  + +   +S+ S  +  C+ C K      +L RH R QH    +H +C  CGKTF  SSGLKQH HIHSS KP+ C  C KSYTQ+SNLCRHKRM  DC+ + KC +CG  F+   + + H++ C G
Sbjct:   20 QHRCEDCDQLFESKAELADHQKFPCSTPHSAFSMVEEDLQQNLESESDLREIHGNQDCKECDRVFPDLQSLEKHMLSHTEEREYKCDQCPKAFNWKSNLIRHQMSHDSGKHYECENCAKVFTDPSNLQRHIRSQHVGARAH-ACPECGKTFATSSGLKQHKHIHSSVKPFICEVCHKSYTQFSNLCRHKRMHADCRTQIKCKDCGQMFSTTSSLNKHRRFCEG 241          

HSP 3 Score: 61.2326 bits (147), Expect = 3.343e-9
Identity = 29/76 (38.16%), Postives = 45/76 (59.21%), Query Frame = 3
Query: 1521 CHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIR 1748
            C  C + FP   +L +H+ +HT E+ YKC  C K F+  SN+ RH  + H+  + Y+C  C ++F   ++L RHIR
Sbjct:   77 CKECDRVFPDLQSLEKHMLSHTEEREYKCDQCPKAFNWKSNLIRHQMS-HDSGKHYECENCAKVFTDPSNLQRHIR 151          

HSP 4 Score: 57.7658 bits (138), Expect = 3.820e-8
Identity = 28/77 (36.36%), Postives = 41/77 (53.25%), Query Frame = 3
Query: 1512 RYHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRH 1742
             Y C  C K F   +NL RH  +H   + Y+C  C K F+  SN+QRH+R+ H   R + C +C + F   + L +H
Sbjct:  102 EYKCDQCPKAFNWKSNLIRHQMSHDSGKHYECENCAKVFTDPSNLQRHIRSQHVGARAHACPECGKTFATSSGLKQH 178          

HSP 5 Score: 55.4546 bits (132), Expect = 1.741e-7
Identity = 24/56 (42.86%), Postives = 36/56 (64.29%), Query Frame = 3
Query: 1587 GEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIRQL 1754
            G++ Y C +C K F  S+N+ RH+R  H  E+PY+C  CDR F   ++L RH+R +
Sbjct:  720 GKERYTCRYCGKIFPRSANLTRHLR-THTGEQPYRCKYCDRSFSISSNLQRHVRNI 774          

HSP 6 Score: 53.5286 bits (127), Expect = 7.796e-7
Identity = 29/80 (36.25%), Postives = 42/80 (52.50%), Query Frame = 3
Query:  657 YSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYTQYSNLCRHKRMSRDCQRKAKCTECGMEFTNGKTYDTH-KQH 893
            Y+C  CGK F  S+ L +H+  H+  +PY C  C +S++  SNL RH R   + ++  KC  C   F      D H K+H
Sbjct:  724 YTCRYCGKIFPRSANLTRHLRTHTGEQPYRCKYCDRSFSISSNLQRHVRNIHNKEKPFKCHLCDRCFGQQTNLDRHLKKH 803          
BLAST of SMED30024013 vs. Ensembl Mouse
Match: Mecom (MDS1 and EVI1 complex locus [Source:MGI Symbol;Acc:MGI:95457])

HSP 1 Score: 131.724 bits (330), Expect = 1.717e-31
Identity = 56/80 (70.00%), Postives = 67/80 (83.75%), Query Frame = 3
Query: 1512 RYHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIRQ 1751
            RY C  C K FPR+ANL RHLRTHTGEQPY+C +CD+ FSISSN+QRHVRNIHNKE+P+KC  CDR F Q T+LDRH+++
Sbjct:  732 RYTCRYCGKIFPRSANLTRHLRTHTGEQPYRCKYCDRSFSISSNLQRHVRNIHNKEKPFKCHLCDRCFGQQTNLDRHLKK 811          

HSP 2 Score: 117.472 bits (293), Expect = 6.234e-27
Identity = 74/223 (33.18%), Postives = 113/223 (50.67%), Query Frame = 3
Query:  276 QFQCPDCGANFSSRGPLKRHQNALCKNPNGLLIKTRQTIFKHNSVIQRKQVLGTNQNCTT-----TNLSGGSEHRKTSSDYKQ--------SVSFPISKLSSPISNGSNLNLFCDVCNKPCRSQVSLIRH-RKQHQNPNSHYSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYTQYSNLCRHKRMSRDCQRKAKCTECGMEFTNGKTYDTHKQHCLG 902
            Q +C DC   F S+  L  HQ   C  P+       + + ++       + +  NQ+C        +L    +H  + ++ ++        + ++  + +   +S+ S  +  C+ C K      +L RH R QH    +H +C  CGKTF  SSGLKQH HIHSS KP+ C  C KSYTQ+SNLCRHKRM  DC+ + KC +CG  F+   + + H++ C G
Sbjct:   20 QHRCEDCDQLFESKAELADHQKFPCSTPHSAFSMVEEDLQQNLESESDLREIHGNQDCKECDRVFPDLQSLEKHMLSHTEEREYKCDQCPKAFNWKSNLIRHQMSHDSGKHYECENCAKVFTDPSNLQRHIRSQHVGARAH-ACPECGKTFATSSGLKQHKHIHSSVKPFICEVCHKSYTQFSNLCRHKRMHADCRTQIKCKDCGQMFSTTSSLNKHRRFCEG 241          

HSP 3 Score: 61.2326 bits (147), Expect = 3.156e-9
Identity = 29/76 (38.16%), Postives = 45/76 (59.21%), Query Frame = 3
Query: 1521 CHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIR 1748
            C  C + FP   +L +H+ +HT E+ YKC  C K F+  SN+ RH  + H+  + Y+C  C ++F   ++L RHIR
Sbjct:   77 CKECDRVFPDLQSLEKHMLSHTEEREYKCDQCPKAFNWKSNLIRHQMS-HDSGKHYECENCAKVFTDPSNLQRHIR 151          

HSP 4 Score: 57.7658 bits (138), Expect = 3.731e-8
Identity = 28/77 (36.36%), Postives = 41/77 (53.25%), Query Frame = 3
Query: 1512 RYHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRH 1742
             Y C  C K F   +NL RH  +H   + Y+C  C K F+  SN+QRH+R+ H   R + C +C + F   + L +H
Sbjct:  102 EYKCDQCPKAFNWKSNLIRHQMSHDSGKHYECENCAKVFTDPSNLQRHIRSQHVGARAHACPECGKTFATSSGLKQH 178          

HSP 5 Score: 55.4546 bits (132), Expect = 1.701e-7
Identity = 24/56 (42.86%), Postives = 36/56 (64.29%), Query Frame = 3
Query: 1587 GEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIRQL 1754
            G++ Y C +C K F  S+N+ RH+R  H  E+PY+C  CDR F   ++L RH+R +
Sbjct:  729 GKERYTCRYCGKIFPRSANLTRHLR-THTGEQPYRCKYCDRSFSISSNLQRHVRNI 783          

HSP 6 Score: 53.5286 bits (127), Expect = 7.233e-7
Identity = 29/80 (36.25%), Postives = 42/80 (52.50%), Query Frame = 3
Query:  657 YSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYTQYSNLCRHKRMSRDCQRKAKCTECGMEFTNGKTYDTH-KQH 893
            Y+C  CGK F  S+ L +H+  H+  +PY C  C +S++  SNL RH R   + ++  KC  C   F      D H K+H
Sbjct:  733 YTCRYCGKIFPRSANLTRHLRTHTGEQPYRCKYCDRSFSISSNLQRHVRNIHNKEKPFKCHLCDRCFGQQTNLDRHLKKH 812          
BLAST of SMED30024013 vs. Ensembl Mouse
Match: Mecom (MDS1 and EVI1 complex locus [Source:MGI Symbol;Acc:MGI:95457])

HSP 1 Score: 131.339 bits (329), Expect = 2.066e-31
Identity = 56/80 (70.00%), Postives = 67/80 (83.75%), Query Frame = 3
Query: 1512 RYHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIRQ 1751
            RY C  C K FPR+ANL RHLRTHTGEQPY+C +CD+ FSISSN+QRHVRNIHNKE+P+KC  CDR F Q T+LDRH+++
Sbjct:  732 RYTCRYCGKIFPRSANLTRHLRTHTGEQPYRCKYCDRSFSISSNLQRHVRNIHNKEKPFKCHLCDRCFGQQTNLDRHLKK 811          

HSP 2 Score: 117.472 bits (293), Expect = 7.341e-27
Identity = 74/223 (33.18%), Postives = 113/223 (50.67%), Query Frame = 3
Query:  276 QFQCPDCGANFSSRGPLKRHQNALCKNPNGLLIKTRQTIFKHNSVIQRKQVLGTNQNCTT-----TNLSGGSEHRKTSSDYKQ--------SVSFPISKLSSPISNGSNLNLFCDVCNKPCRSQVSLIRH-RKQHQNPNSHYSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYTQYSNLCRHKRMSRDCQRKAKCTECGMEFTNGKTYDTHKQHCLG 902
            Q +C DC   F S+  L  HQ   C  P+       + + ++       + +  NQ+C        +L    +H  + ++ ++        + ++  + +   +S+ S  +  C+ C K      +L RH R QH    +H +C  CGKTF  SSGLKQH HIHSS KP+ C  C KSYTQ+SNLCRHKRM  DC+ + KC +CG  F+   + + H++ C G
Sbjct:   20 QHRCEDCDQLFESKAELADHQKFPCSTPHSAFSMVEEDLQQNLESESDLREIHGNQDCKECDRVFPDLQSLEKHMLSHTEEREYKCDQCPKAFNWKSNLIRHQMSHDSGKHYECENCAKVFTDPSNLQRHIRSQHVGARAH-ACPECGKTFATSSGLKQHKHIHSSVKPFICEVCHKSYTQFSNLCRHKRMHADCRTQIKCKDCGQMFSTTSSLNKHRRFCEG 241          

HSP 3 Score: 60.8474 bits (146), Expect = 3.538e-9
Identity = 29/76 (38.16%), Postives = 45/76 (59.21%), Query Frame = 3
Query: 1521 CHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIR 1748
            C  C + FP   +L +H+ +HT E+ YKC  C K F+  SN+ RH  + H+  + Y+C  C ++F   ++L RHIR
Sbjct:   77 CKECDRVFPDLQSLEKHMLSHTEEREYKCDQCPKAFNWKSNLIRHQMS-HDSGKHYECENCAKVFTDPSNLQRHIR 151          

HSP 4 Score: 57.7658 bits (138), Expect = 3.882e-8
Identity = 28/77 (36.36%), Postives = 41/77 (53.25%), Query Frame = 3
Query: 1512 RYHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRH 1742
             Y C  C K F   +NL RH  +H   + Y+C  C K F+  SN+QRH+R+ H   R + C +C + F   + L +H
Sbjct:  102 EYKCDQCPKAFNWKSNLIRHQMSHDSGKHYECENCAKVFTDPSNLQRHIRSQHVGARAHACPECGKTFATSSGLKQH 178          

HSP 5 Score: 55.4546 bits (132), Expect = 1.850e-7
Identity = 24/56 (42.86%), Postives = 36/56 (64.29%), Query Frame = 3
Query: 1587 GEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIRQL 1754
            G++ Y C +C K F  S+N+ RH+R  H  E+PY+C  CDR F   ++L RH+R +
Sbjct:  729 GKERYTCRYCGKIFPRSANLTRHLR-THTGEQPYRCKYCDRSFSISSNLQRHVRNI 783          

HSP 6 Score: 53.5286 bits (127), Expect = 6.580e-7
Identity = 29/80 (36.25%), Postives = 42/80 (52.50%), Query Frame = 3
Query:  657 YSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYTQYSNLCRHKRMSRDCQRKAKCTECGMEFTNGKTYDTH-KQH 893
            Y+C  CGK F  S+ L +H+  H+  +PY C  C +S++  SNL RH R   + ++  KC  C   F      D H K+H
Sbjct:  733 YTCRYCGKIFPRSANLTRHLRTHTGEQPYRCKYCDRSFSISSNLQRHVRNIHNKEKPFKCHLCDRCFGQQTNLDRHLKKH 812          
BLAST of SMED30024013 vs. UniProt/SwissProt
Match: sp|Q8I7Z8|HAM_DROME (Transcription factor hamlet OS=Drosophila melanogaster OX=7227 GN=ham PE=2 SV=1)

HSP 1 Score: 132.494 bits (332), Expect = 7.310e-31
Identity = 56/80 (70.00%), Postives = 66/80 (82.50%), Query Frame = 3
Query: 1512 RYHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIRQ 1751
            RY C  C K FPR+ANL RHLRTHTGEQPY C +CD+ FSISSN+QRHVRNIHNKERP++C  CDR F Q T+LDRH+++
Sbjct:  816 RYSCKFCGKVFPRSANLTRHLRTHTGEQPYPCKYCDRAFSISSNLQRHVRNIHNKERPFRCELCDRSFGQQTNLDRHVKK 895          

HSP 2 Score: 100.138 bits (248), Expect = 1.546e-20
Identity = 52/117 (44.44%), Postives = 66/117 (56.41%), Query Frame = 3
Query:  570 LFCDVCNKPCRSQVSLIRHRKQHQNPNSHYSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYTQYSNLCRHKRMSRDCQRKAKCTECGMEFTNGKTYDTHKQHCLGLDPSGY 920
            +FCD  N        L RH + +      + C  CGKTF  SSGLKQH HIHSS KP++C  C K+YTQ+SNLCRHKRM   C+ + KC +C   F+   +   HK+ C    P  Y
Sbjct:  288 VFCDPSN--------LQRHIRAYHVGARCHPCPECGKTFGTSSGLKQHQHIHSSVKPFACEVCSKAYTQFSNLCRHKRMHATCRMQIKCDKCNQSFSTLTSLTKHKKFCDSTGPGPY 396          

HSP 3 Score: 58.151 bits (139), Expect = 2.200e-7
Identity = 29/80 (36.25%), Postives = 42/80 (52.50%), Query Frame = 3
Query:  657 YSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYTQYSNLCRHKRMSRDCQRKAKCTECGMEFTNGKTYDTH-KQH 893
            YSC  CGK F  S+ L +H+  H+  +PY C  C ++++  SNL RH R   + +R  +C  C   F      D H K+H
Sbjct:  817 YSCKFCGKVFPRSANLTRHLRTHTGEQPYPCKYCDRAFSISSNLQRHVRNIHNKERPFRCELCDRSFGQQTNLDRHVKKH 896          

HSP 4 Score: 56.9954 bits (136), Expect = 5.055e-7
Identity = 29/80 (36.25%), Postives = 46/80 (57.50%), Query Frame = 3
Query: 1512 RYHCHLCLKTFPRAANLNRHLRT-HTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIR 1748
            +Y C  C K F   +NL RH+R  H G + + C  C K F  SS +++H ++IH+  +P+ C  C + + Q ++L RH R
Sbjct:  279 KYSCENCSKVFCDPSNLQRHIRAYHVGARCHPCPECGKTFGTSSGLKQH-QHIHSSVKPFACEVCSKAYTQFSNLCRHKR 357          

HSP 5 Score: 55.0694 bits (131), Expect = 1.976e-6
Identity = 24/52 (46.15%), Postives = 32/52 (61.54%), Query Frame = 3
Query: 1599 YKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIRQL 1754
            Y C FC K F  S+N+ RH+R  H  E+PY C  CDR F   ++L RH+R +
Sbjct:  817 YSCKFCGKVFPRSANLTRHLR-THTGEQPYPCKYCDRAFSISSNLQRHVRNI 867          
BLAST of SMED30024013 vs. UniProt/SwissProt
Match: sp|Q03112|MECOM_HUMAN (Histone-lysine N-methyltransferase MECOM OS=Homo sapiens OX=9606 GN=MECOM PE=1 SV=3)

HSP 1 Score: 132.109 bits (331), Expect = 1.084e-30
Identity = 56/80 (70.00%), Postives = 67/80 (83.75%), Query Frame = 3
Query: 1512 RYHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIRQ 1751
            RY C  C K FPR+ANL RHLRTHTGEQPY+C +CD+ FSISSN+QRHVRNIHNKE+P+KC  CDR F Q T+LDRH+++
Sbjct:  911 RYTCRYCGKIFPRSANLTRHLRTHTGEQPYRCKYCDRSFSISSNLQRHVRNIHNKEKPFKCHLCDRCFGQQTNLDRHLKK 990          

HSP 2 Score: 119.013 bits (297), Expect = 1.653e-26
Identity = 75/223 (33.63%), Postives = 111/223 (49.78%), Query Frame = 3
Query:  276 QFQCPDCGANFSSRGPLKRHQNALCKNPNGLLIKTRQTIFKHNSVIQRKQVLGTNQNCTT-----TNLSGGSEHRKTSSDYKQ--------SVSFPISKLSSPISNGSNLNLFCDVCNKPCRSQVSLIRH-RKQHQNPNSHYSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYTQYSNLCRHKRMSRDCQRKAKCTECGMEFTNGKTYDTHKQHCLG 902
            Q++C DC   F S+  L  HQ   C  P+       +   +        Q + T Q C        +L    +H  + ++ ++        + ++  + +   +S+ S  +  C+ C K      +L RH R QH    +H +C  CGKTF  SSGLKQH HIHSS KP+ C  C KSYTQ+SNLCRHKRM  DC+ + KC +CG  F+   + + H++ C G
Sbjct:  208 QYRCEDCDQLFESKAELADHQKFPCSTPHSAFSMVEEDFQQKLESENDLQEIHTIQECKECDQVFPDLQSLEKHMLSHTEEREYKCDQCPKAFNWKSNLIRHQMSHDSGKHYECENCAKVFTDPSNLQRHIRSQHVGARAH-ACPECGKTFATSSGLKQHKHIHSSVKPFICEVCHKSYTQFSNLCRHKRMHADCRTQIKCKDCGQMFSTTSSLNKHRRFCEG 429          

HSP 3 Score: 61.2326 bits (147), Expect = 2.262e-8
Identity = 29/76 (38.16%), Postives = 45/76 (59.21%), Query Frame = 3
Query: 1521 CHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIR 1748
            C  C + FP   +L +H+ +HT E+ YKC  C K F+  SN+ RH  + H+  + Y+C  C ++F   ++L RHIR
Sbjct:  265 CKECDQVFPDLQSLEKHMLSHTEEREYKCDQCPKAFNWKSNLIRHQMS-HDSGKHYECENCAKVFTDPSNLQRHIR 339          

HSP 4 Score: 58.151 bits (139), Expect = 1.992e-7
Identity = 28/77 (36.36%), Postives = 41/77 (53.25%), Query Frame = 3
Query: 1512 RYHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRH 1742
             Y C  C K F   +NL RH  +H   + Y+C  C K F+  SN+QRH+R+ H   R + C +C + F   + L +H
Sbjct:  290 EYKCDQCPKAFNWKSNLIRHQMSHDSGKHYECENCAKVFTDPSNLQRHIRSQHVGARAHACPECGKTFATSSGLKQH 366          

HSP 5 Score: 55.8398 bits (133), Expect = 1.076e-6
Identity = 24/56 (42.86%), Postives = 36/56 (64.29%), Query Frame = 3
Query: 1587 GEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIRQL 1754
            G++ Y C +C K F  S+N+ RH+R  H  E+PY+C  CDR F   ++L RH+R +
Sbjct:  908 GKERYTCRYCGKIFPRSANLTRHLRT-HTGEQPYRCKYCDRSFSISSNLQRHVRNI 962          

HSP 6 Score: 53.9138 bits (128), Expect = 4.375e-6
Identity = 29/80 (36.25%), Postives = 42/80 (52.50%), Query Frame = 3
Query:  657 YSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYTQYSNLCRHKRMSRDCQRKAKCTECGMEFTNGKTYDTH-KQH 893
            Y+C  CGK F  S+ L +H+  H+  +PY C  C +S++  SNL RH R   + ++  KC  C   F      D H K+H
Sbjct:  912 YTCRYCGKIFPRSANLTRHLRTHTGEQPYRCKYCDRSFSISSNLQRHVRNIHNKEKPFKCHLCDRCFGQQTNLDRHLKKH 991          
BLAST of SMED30024013 vs. UniProt/SwissProt
Match: sp|P14404|MECOM_MOUSE (Histone-lysine N-methyltransferase MECOM OS=Mus musculus OX=10090 GN=Mecom PE=1 SV=2)

HSP 1 Score: 131.724 bits (330), Expect = 1.322e-30
Identity = 56/80 (70.00%), Postives = 67/80 (83.75%), Query Frame = 3
Query: 1512 RYHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIRQ 1751
            RY C  C K FPR+ANL RHLRTHTGEQPY+C +CD+ FSISSN+QRHVRNIHNKE+P+KC  CDR F Q T+LDRH+++
Sbjct:  913 RYTCRYCGKIFPRSANLTRHLRTHTGEQPYRCKYCDRSFSISSNLQRHVRNIHNKEKPFKCHLCDRCFGQQTNLDRHLKK 992          

HSP 2 Score: 117.857 bits (294), Expect = 3.984e-26
Identity = 74/223 (33.18%), Postives = 113/223 (50.67%), Query Frame = 3
Query:  276 QFQCPDCGANFSSRGPLKRHQNALCKNPNGLLIKTRQTIFKHNSVIQRKQVLGTNQNCTT-----TNLSGGSEHRKTSSDYKQ--------SVSFPISKLSSPISNGSNLNLFCDVCNKPCRSQVSLIRH-RKQHQNPNSHYSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYTQYSNLCRHKRMSRDCQRKAKCTECGMEFTNGKTYDTHKQHCLG 902
            Q +C DC   F S+  L  HQ   C  P+       + + ++       + +  NQ+C        +L    +H  + ++ ++        + ++  + +   +S+ S  +  C+ C K      +L RH R QH    +H +C  CGKTF  SSGLKQH HIHSS KP+ C  C KSYTQ+SNLCRHKRM  DC+ + KC +CG  F+   + + H++ C G
Sbjct:  210 QHRCEDCDQLFESKAELADHQKFPCSTPHSAFSMVEEDLQQNLESESDLREIHGNQDCKECDRVFPDLQSLEKHMLSHTEEREYKCDQCPKAFNWKSNLIRHQMSHDSGKHYECENCAKVFTDPSNLQRHIRSQHVGARAH-ACPECGKTFATSSGLKQHKHIHSSVKPFICEVCHKSYTQFSNLCRHKRMHADCRTQIKCKDCGQMFSTTSSLNKHRRFCEG 431          

HSP 3 Score: 61.6178 bits (148), Expect = 1.990e-8
Identity = 29/76 (38.16%), Postives = 45/76 (59.21%), Query Frame = 3
Query: 1521 CHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIR 1748
            C  C + FP   +L +H+ +HT E+ YKC  C K F+  SN+ RH  + H+  + Y+C  C ++F   ++L RHIR
Sbjct:  267 CKECDRVFPDLQSLEKHMLSHTEEREYKCDQCPKAFNWKSNLIRHQMS-HDSGKHYECENCAKVFTDPSNLQRHIR 341          

HSP 4 Score: 58.151 bits (139), Expect = 2.345e-7
Identity = 28/77 (36.36%), Postives = 41/77 (53.25%), Query Frame = 3
Query: 1512 RYHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRH 1742
             Y C  C K F   +NL RH  +H   + Y+C  C K F+  SN+QRH+R+ H   R + C +C + F   + L +H
Sbjct:  292 EYKCDQCPKAFNWKSNLIRHQMSHDSGKHYECENCAKVFTDPSNLQRHIRSQHVGARAHACPECGKTFATSSGLKQH 368          

HSP 5 Score: 55.8398 bits (133), Expect = 1.223e-6
Identity = 24/56 (42.86%), Postives = 36/56 (64.29%), Query Frame = 3
Query: 1587 GEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIRQL 1754
            G++ Y C +C K F  S+N+ RH+R  H  E+PY+C  CDR F   ++L RH+R +
Sbjct:  910 GKERYTCRYCGKIFPRSANLTRHLRT-HTGEQPYRCKYCDRSFSISSNLQRHVRNI 964          

HSP 6 Score: 53.5286 bits (127), Expect = 5.061e-6
Identity = 29/80 (36.25%), Postives = 42/80 (52.50%), Query Frame = 3
Query:  657 YSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYTQYSNLCRHKRMSRDCQRKAKCTECGMEFTNGKTYDTH-KQH 893
            Y+C  CGK F  S+ L +H+  H+  +PY C  C +S++  SNL RH R   + ++  KC  C   F      D H K+H
Sbjct:  914 YTCRYCGKIFPRSANLTRHLRTHTGEQPYRCKYCDRSFSISSNLQRHVRNIHNKEKPFKCHLCDRCFGQQTNLDRHLKKH 993          
BLAST of SMED30024013 vs. UniProt/SwissProt
Match: sp|B7ZRU9|EVI1A_XENLA (MDS1 and EVI1 complex locus protein EVI1-A OS=Xenopus laevis OX=8355 GN=mecom-a PE=1 SV=1)

HSP 1 Score: 131.339 bits (329), Expect = 1.620e-30
Identity = 56/80 (70.00%), Postives = 67/80 (83.75%), Query Frame = 3
Query: 1512 RYHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIRQ 1751
            RY C  C K FPR+ANL RHLRTHTGEQPY+C +CD+ FSISSN+QRHVRNIHNKE+P+KC  CDR F Q T+LDRH+++
Sbjct:  733 RYTCRYCGKIFPRSANLTRHLRTHTGEQPYRCKYCDRSFSISSNLQRHVRNIHNKEKPFKCHLCDRCFGQQTNLDRHLKK 812          

HSP 2 Score: 111.694 bits (278), Expect = 3.563e-24
Identity = 77/224 (34.38%), Postives = 113/224 (50.45%), Query Frame = 3
Query:  276 QFQCPDCGANFSSRGPLKRHQNALCKNPNGL--LIK----------TRQTIFKHNSVIQR-KQVLGTNQNCTTTNLSGGSEHRKTSSDYKQSVSFPISKLSSPISNGSNLNLFCDVCNKPCRSQVS-LIRH-RKQHQNPNSHYSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYTQYSNLCRHKRMSRDCQRKAKCTECGMEFTNGKTYDTHKQHCLG 902
            Q++C +C   F S+  L  HQ   C  P+    L++          +  T  +H    +   QV    Q+     LS   E         ++ ++  + +   +S+ +  +  C+ C+K   +  S L RH R QH    +H +C  CGKTF  SSGLKQH HIHSS KP+ C  C KSYTQ+SNLCRHKRM  DC+ + KC +CG  F+   + + H++ C G
Sbjct:   20 QYRCEECDQLFESKTELSDHQKYPCVTPHSAFSLVENSFPPSLNDDSDLTEMQHTHECKECDQVFPDMQSLEKHLLSHTEEREYKCDQCPKAFNWKSNLIRHQMSHDTGKHYECENCSKQVFTDPSNLQRHIRSQHVGARAH-ACSDCGKTFATSSGLKQHKHIHSSVKPFVCEVCHKSYTQFSNLCRHKRMHADCRTQIKCKDCGQMFSTTSSLNKHRRFCEG 242          

HSP 3 Score: 57.7658 bits (138), Expect = 2.378e-7
Identity = 30/79 (37.97%), Postives = 46/79 (58.23%), Query Frame = 3
Query: 1515 YHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDR-IFDQLTSLDRHIR 1748
            + C  C + FP   +L +HL +HT E+ YKC  C K F+  SN+ RH  + H+  + Y+C  C + +F   ++L RHIR
Sbjct:   75 HECKECDQVFPDMQSLEKHLLSHTEEREYKCDQCPKAFNWKSNLIRHQMS-HDTGKHYECENCSKQVFTDPSNLQRHIR 152          

HSP 4 Score: 55.8398 bits (133), Expect = 1.093e-6
Identity = 24/56 (42.86%), Postives = 36/56 (64.29%), Query Frame = 3
Query: 1587 GEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIRQL 1754
            G++ Y C +C K F  S+N+ RH+R  H  E+PY+C  CDR F   ++L RH+R +
Sbjct:  730 GKERYTCRYCGKIFPRSANLTRHLR-THTGEQPYRCKYCDRSFSISSNLQRHVRNI 784          

HSP 5 Score: 53.9138 bits (128), Expect = 4.648e-6
Identity = 30/83 (36.14%), Postives = 43/83 (51.81%), Query Frame = 3
Query:  657 YSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYTQYSNLCRHKRMSRDCQRKAKCTECGMEFTNGKTYDTH-KQHCLG 902
            Y+C  CGK F  S+ L +H+  H+  +PY C  C +S++  SNL RH R   + ++  KC  C   F      D H K+H  G
Sbjct:  734 YTCRYCGKIFPRSANLTRHLRTHTGEQPYRCKYCDRSFSISSNLQRHVRNIHNKEKPFKCHLCDRCFGQQTNLDRHLKKHENG 816          

HSP 6 Score: 53.1434 bits (126), Expect = 6.166e-6
Identity = 28/78 (35.90%), Postives = 41/78 (52.56%), Query Frame = 3
Query: 1512 RYHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDK-CFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRH 1742
             Y C  C K F   +NL RH  +H   + Y+C  C K  F+  SN+QRH+R+ H   R + C+ C + F   + L +H
Sbjct:  102 EYKCDQCPKAFNWKSNLIRHQMSHDTGKHYECENCSKQVFTDPSNLQRHIRSQHVGARAHACSDCGKTFATSSGLKQH 179          
BLAST of SMED30024013 vs. UniProt/SwissProt
Match: sp|B7ZRM8|EVI1B_XENLA (MDS1 and EVI1 complex locus protein EVI1-B OS=Xenopus laevis OX=8355 GN=mecom-b PE=2 SV=1)

HSP 1 Score: 130.568 bits (327), Expect = 2.668e-30
Identity = 55/80 (68.75%), Postives = 67/80 (83.75%), Query Frame = 3
Query: 1512 RYHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIRQ 1751
            RY C  C K FPR+ANL RHLRTHTGEQPY+C +CD+ FSISSN+QRH+RNIHNKE+P+KC  CDR F Q T+LDRH+++
Sbjct:  730 RYTCRYCGKIFPRSANLTRHLRTHTGEQPYRCKYCDRSFSISSNLQRHIRNIHNKEKPFKCHLCDRCFGQQTNLDRHLKK 809          

HSP 2 Score: 114.005 bits (284), Expect = 5.798e-25
Identity = 77/224 (34.38%), Postives = 111/224 (49.55%), Query Frame = 3
Query:  276 QFQCPDCGANFSSRGPLKRHQNALCKNPNG---LLIKTRQTIFKHNSVIQR----------KQVLGTNQNCTTTNLSGGSEHRKTSSDYKQSVSFPISKLSSPISNGSNLNLFCDVCNKPCRSQVS-LIRH-RKQHQNPNSHYSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYTQYSNLCRHKRMSRDCQRKAKCTECGMEFTNGKTYDTHKQHCLG 902
            Q+ C +C   F S+  L  HQ   C  P+    L+  +   I   +S +             QV    Q+     LS   E         ++ ++  + +   +S+ +  +  C+ C+K   +  S L RH R QH    +H +C  CGKTF  SSGLKQH HIHSS KP+ C  C KSYTQ+SNLCRHKRM  DC+ + KC +CG  F+   + + H++ C G
Sbjct:   20 QYHCEECDQLFESKTELSNHQKYSCGTPHSAFSLVENSFPPILNDDSDLTEMQHAHECKECDQVFPDMQSLEKHLLSHTEEREYKCDQCPKAFNWKSNLIRHQMSHDTGKHYECENCSKQVFTDPSNLQRHIRSQHVGARAH-ACSECGKTFATSSGLKQHKHIHSSVKPFVCEVCHKSYTQFSNLCRHKRMHADCRTQIKCKDCGQMFSTTSSLNKHRRFCEG 242          

HSP 3 Score: 57.7658 bits (138), Expect = 2.747e-7
Identity = 30/79 (37.97%), Postives = 46/79 (58.23%), Query Frame = 3
Query: 1515 YHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDR-IFDQLTSLDRHIR 1748
            + C  C + FP   +L +HL +HT E+ YKC  C K F+  SN+ RH  + H+  + Y+C  C + +F   ++L RHIR
Sbjct:   75 HECKECDQVFPDMQSLEKHLLSHTEEREYKCDQCPKAFNWKSNLIRHQMS-HDTGKHYECENCSKQVFTDPSNLQRHIR 152          

HSP 4 Score: 56.225 bits (134), Expect = 7.113e-7
Identity = 25/56 (44.64%), Postives = 36/56 (64.29%), Query Frame = 3
Query: 1587 GEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIRQL 1754
            G++ Y C +C K F  S+N+ RH+R  H  E+PY+C  CDR F   ++L RHIR +
Sbjct:  727 GKERYTCRYCGKIFPRSANLTRHLR-THTGEQPYRCKYCDRSFSISSNLQRHIRNI 781          

HSP 5 Score: 53.9138 bits (128), Expect = 4.014e-6
Identity = 28/78 (35.90%), Postives = 42/78 (53.85%), Query Frame = 3
Query: 1512 RYHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDK-CFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRH 1742
             Y C  C K F   +NL RH  +H   + Y+C  C K  F+  SN+QRH+R+ H   R + C++C + F   + L +H
Sbjct:  102 EYKCDQCPKAFNWKSNLIRHQMSHDTGKHYECENCSKQVFTDPSNLQRHIRSQHVGARAHACSECGKTFATSSGLKQH 179          

HSP 6 Score: 53.1434 bits (126), Expect = 6.768e-6
Identity = 30/83 (36.14%), Postives = 43/83 (51.81%), Query Frame = 3
Query:  657 YSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYTQYSNLCRHKRMSRDCQRKAKCTECGMEFTNGKTYDTH-KQHCLG 902
            Y+C  CGK F  S+ L +H+  H+  +PY C  C +S++  SNL RH R   + ++  KC  C   F      D H K+H  G
Sbjct:  731 YTCRYCGKIFPRSANLTRHLRTHTGEQPYRCKYCDRSFSISSNLQRHIRNIHNKEKPFKCHLCDRCFGQQTNLDRHLKKHENG 813          
BLAST of SMED30024013 vs. TrEMBL
Match: A0A0B1S9I4 (Zinc finger, C2H2 type OS=Oesophagostomum dentatum OX=61180 GN=OESDEN_18342 PE=4 SV=1)

HSP 1 Score: 131.339 bits (329), Expect = 3.613e-32
Identity = 56/80 (70.00%), Postives = 68/80 (85.00%), Query Frame = 3
Query: 1512 RYHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIRQ 1751
            RY C  C K FPR+ANL RHLRTHTGEQPYKC +C++ FSISSN+QRHVRNIHNKERP++C +CDR F Q T+LDRH+++
Sbjct:   48 RYTCKFCQKVFPRSANLTRHLRTHTGEQPYKCQYCERSFSISSNLQRHVRNIHNKERPFRCPRCDRCFGQQTNLDRHLKK 127          
BLAST of SMED30024013 vs. TrEMBL
Match: A0A0C2D5P8 (Zinc finger, C2H2 type (Fragment) OS=Ancylostoma duodenale OX=51022 GN=ANCDUO_04811 PE=4 SV=1)

HSP 1 Score: 130.568 bits (327), Expect = 4.690e-32
Identity = 56/80 (70.00%), Postives = 68/80 (85.00%), Query Frame = 3
Query: 1512 RYHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIRQ 1751
            RY C  C K FPR+ANL RHLRTHTGEQPYKC +C++ FSISSN+QRHVRNIHNKERP++C +CDR F Q T+LDRH+++
Sbjct:   33 RYTCKFCQKVFPRSANLTRHLRTHTGEQPYKCQYCERSFSISSNLQRHVRNIHNKERPFRCPRCDRCFGQQTNLDRHLKK 112          
BLAST of SMED30024013 vs. TrEMBL
Match: A0A1L8DNR8 (Putative conserved secreted protein OS=Nyssomyia neivai OX=330878 PE=4 SV=1)

HSP 1 Score: 132.109 bits (331), Expect = 1.117e-31
Identity = 56/80 (70.00%), Postives = 67/80 (83.75%), Query Frame = 3
Query: 1512 RYHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIRQ 1751
            RY C  C K FPR+ANL RHLRTHTGEQPYKC +C++ FSISSN+QRHVRNIHNKERP+KC  C+R F Q T+LDRH+++
Sbjct:   31 RYACKFCGKVFPRSANLTRHLRTHTGEQPYKCKYCERSFSISSNLQRHVRNIHNKERPFKCHLCERCFGQQTNLDRHLKK 110          
BLAST of SMED30024013 vs. TrEMBL
Match: A0A0D6LYC1 (Zinc finger, C2H2 type OS=Ancylostoma ceylanicum OX=53326 GN=ANCCEY_03889 PE=4 SV=1)

HSP 1 Score: 133.65 bits (335), Expect = 1.729e-31
Identity = 56/80 (70.00%), Postives = 68/80 (85.00%), Query Frame = 3
Query: 1512 RYHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIRQ 1751
            RY C  C K FPR+ANL RHLRTHTGEQPYKC +C++ FSISSN+QRHVRNIHNKERP++C +CDR F Q T+LDRH+++
Sbjct:  172 RYTCKFCQKVFPRSANLTRHLRTHTGEQPYKCQYCERSFSISSNLQRHVRNIHNKERPFRCPRCDRCFGQQTNLDRHLKK 251          
BLAST of SMED30024013 vs. TrEMBL
Match: Q4RE09 (Chromosome undetermined SCAF15202, whole genome shotgun sequence (Fragment) OS=Tetraodon nigroviridis OX=99883 GN=GSTENG00036656001 PE=4 SV=1)

HSP 1 Score: 129.413 bits (324), Expect = 1.853e-31
Identity = 56/80 (70.00%), Postives = 67/80 (83.75%), Query Frame = 3
Query: 1512 RYHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIRQ 1751
            RY C  C K FPR+ANL RHLRTHTGEQPY+C FCD+ FSISSN+QRH+RNIHNKE+P+KC  CDR F Q T+LDRH+++
Sbjct:   51 RYACRYCGKIFPRSANLTRHLRTHTGEQPYRCKFCDRSFSISSNLQRHIRNIHNKEKPFKCHLCDRCFGQQTNLDRHLKK 130          
BLAST of SMED30024013 vs. Ensembl Cavefish
Match: mecom (MDS1 and EVI1 complex locus [Source:ZFIN;Acc:ZDB-GENE-050208-123])

HSP 1 Score: 130.954 bits (328), Expect = 2.827e-31
Identity = 55/80 (68.75%), Postives = 67/80 (83.75%), Query Frame = 3
Query: 1512 RYHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIRQ 1751
            RY C  C K FPR+ANL RHLRTHTGEQPY+C +CD+ FSISSN+QRH+RNIHNKE+P+KC  CDR F Q T+LDRH+++
Sbjct:  729 RYTCRYCGKIFPRSANLTRHLRTHTGEQPYRCKYCDRSFSISSNLQRHIRNIHNKEKPFKCHLCDRCFGQQTNLDRHLKK 808          

HSP 2 Score: 112.849 bits (281), Expect = 1.452e-25
Identity = 77/229 (33.62%), Postives = 111/229 (48.47%), Query Frame = 3
Query:  276 QFQCPDCGANFSSRGPLKRHQNALCKN---------------PNGLLIKTRQTIFKHNSVIQR----KQVLGTNQNCTTTNLSGGSEHRKTSSDYKQSVSFPISKLSSPISNGSNLNLFCDVCNKPCRSQVSLIRH-RKQHQNPNSHYSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYTQYSNLCRHKRMSRDCQRKAKCTECGMEFTNGKTYDTHKQHCLG 902
            +F+C DC  +FSS   L  HQ   C +                +G   +    +       Q     +QV    Q+    +LS   E         ++ ++  + +   +S+ S  +  C+ C+K      +L RH R QH    +H +C  CGKTF  SSGLKQH HIHSS KP+ C  C KSYTQ+SNLCRHKRM  DC+ + KC +CG  F+   + + H++ C G
Sbjct:   20 EFRCEDCEQHFSSGPELIEHQKESCSSRLHSHFILPEQDVPLSDGENTELHPLLLHPPDSPQECKECEQVFPDTQSLEAHSLSHSEEREYKCDQCPKAFNWKSNLIRHQMSHDSGKHYECENCSKVFTDPSNLQRHIRSQHVGARAH-ACPDCGKTFATSSGLKQHKHIHSSVKPFICEVCHKSYTQFSNLCRHKRMHADCRTQIKCKDCGQMFSTTSSLNKHRRFCEG 247          

HSP 3 Score: 57.7658 bits (138), Expect = 3.150e-8
Identity = 28/77 (36.36%), Postives = 40/77 (51.95%), Query Frame = 3
Query: 1512 RYHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRH 1742
             Y C  C K F   +NL RH  +H   + Y+C  C K F+  SN+QRH+R+ H   R + C  C + F   + L +H
Sbjct:  108 EYKCDQCPKAFNWKSNLIRHQMSHDSGKHYECENCSKVFTDPSNLQRHIRSQHVGARAHACPDCGKTFATSSGLKQH 184          

HSP 4 Score: 56.9954 bits (136), Expect = 4.961e-8
Identity = 28/76 (36.84%), Postives = 43/76 (56.58%), Query Frame = 3
Query: 1521 CHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIR 1748
            C  C + FP   +L  H  +H+ E+ YKC  C K F+  SN+ RH  + H+  + Y+C  C ++F   ++L RHIR
Sbjct:   83 CKECEQVFPDTQSLEAHSLSHSEEREYKCDQCPKAFNWKSNLIRHQMS-HDSGKHYECENCSKVFTDPSNLQRHIR 157          

HSP 5 Score: 56.225 bits (134), Expect = 8.886e-8
Identity = 25/56 (44.64%), Postives = 36/56 (64.29%), Query Frame = 3
Query: 1587 GEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIRQL 1754
            G++ Y C +C K F  S+N+ RH+R  H  E+PY+C  CDR F   ++L RHIR +
Sbjct:  726 GKERYTCRYCGKIFPRSANLTRHLR-THTGEQPYRCKYCDRSFSISSNLQRHIRNI 780          

HSP 6 Score: 53.1434 bits (126), Expect = 8.457e-7
Identity = 29/80 (36.25%), Postives = 42/80 (52.50%), Query Frame = 3
Query:  657 YSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYTQYSNLCRHKRMSRDCQRKAKCTECGMEFTNGKTYDTH-KQH 893
            Y+C  CGK F  S+ L +H+  H+  +PY C  C +S++  SNL RH R   + ++  KC  C   F      D H K+H
Sbjct:  730 YTCRYCGKIFPRSANLTRHLRTHTGEQPYRCKYCDRSFSISSNLQRHIRNIHNKEKPFKCHLCDRCFGQQTNLDRHLKKH 809          
BLAST of SMED30024013 vs. Ensembl Cavefish
Match: mecom (MDS1 and EVI1 complex locus [Source:ZFIN;Acc:ZDB-GENE-050208-123])

HSP 1 Score: 130.568 bits (327), Expect = 3.471e-31
Identity = 55/80 (68.75%), Postives = 67/80 (83.75%), Query Frame = 3
Query: 1512 RYHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIRQ 1751
            RY C  C K FPR+ANL RHLRTHTGEQPY+C +CD+ FSISSN+QRH+RNIHNKE+P+KC  CDR F Q T+LDRH+++
Sbjct:  755 RYTCRYCGKIFPRSANLTRHLRTHTGEQPYRCKYCDRSFSISSNLQRHIRNIHNKEKPFKCHLCDRCFGQQTNLDRHLKK 834          

HSP 2 Score: 110.923 bits (276), Expect = 6.013e-25
Identity = 78/230 (33.91%), Postives = 112/230 (48.70%), Query Frame = 3
Query:  276 QFQCPDCGANFSSRGPLKRHQNALCKN---------------PNGLLIKTRQTIFKHNSVIQR----KQVLGTNQNCTTTNLSGGSEHRKTSSDYKQSVSFPISKLSSPISNGSNLNLFCDVCNKPCRSQVS-LIRH-RKQHQNPNSHYSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYTQYSNLCRHKRMSRDCQRKAKCTECGMEFTNGKTYDTHKQHCLG 902
            +F+C DC  +FSS   L  HQ   C +                +G   +    +       Q     +QV    Q+    +LS   E         ++ ++  + +   +S+ S  +  C+ C+K   +  S L RH R QH    +H +C  CGKTF  SSGLKQH HIHSS KP+ C  C KSYTQ+SNLCRHKRM  DC+ + KC +CG  F+   + + H++ C G
Sbjct:   20 EFRCEDCEQHFSSGPELIEHQKESCSSRLHSHFILPEQDVPLSDGENTELHPLLLHPPDSPQECKECEQVFPDTQSLEAHSLSHSEEREYKCDQCPKAFNWKSNLIRHQMSHDSGKHYECENCSKQVFTDPSNLQRHIRSQHVGARAH-ACPDCGKTFATSSGLKQHKHIHSSVKPFICEVCHKSYTQFSNLCRHKRMHADCRTQIKCKDCGQMFSTTSSLNKHRRFCEG 248          

HSP 3 Score: 56.225 bits (134), Expect = 9.708e-8
Identity = 25/56 (44.64%), Postives = 36/56 (64.29%), Query Frame = 3
Query: 1587 GEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIRQL 1754
            G++ Y C +C K F  S+N+ RH+R  H  E+PY+C  CDR F   ++L RHIR +
Sbjct:  752 GKERYTCRYCGKIFPRSANLTRHLR-THTGEQPYRCKYCDRSFSISSNLQRHIRNI 806          

HSP 4 Score: 53.1434 bits (126), Expect = 7.206e-7
Identity = 29/80 (36.25%), Postives = 42/80 (52.50%), Query Frame = 3
Query:  657 YSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYTQYSNLCRHKRMSRDCQRKAKCTECGMEFTNGKTYDTH-KQH 893
            Y+C  CGK F  S+ L +H+  H+  +PY C  C +S++  SNL RH R   + ++  KC  C   F      D H K+H
Sbjct:  756 YTCRYCGKIFPRSANLTRHLRTHTGEQPYRCKYCDRSFSISSNLQRHIRNIHNKEKPFKCHLCDRCFGQQTNLDRHLKKH 835          

HSP 5 Score: 52.7582 bits (125), Expect = 9.158e-7
Identity = 28/78 (35.90%), Postives = 40/78 (51.28%), Query Frame = 3
Query: 1512 RYHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDK-CFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRH 1742
             Y C  C K F   +NL RH  +H   + Y+C  C K  F+  SN+QRH+R+ H   R + C  C + F   + L +H
Sbjct:  108 EYKCDQCPKAFNWKSNLIRHQMSHDSGKHYECENCSKQVFTDPSNLQRHIRSQHVGARAHACPDCGKTFATSSGLKQH 185          

HSP 6 Score: 52.373 bits (124), Expect = 1.393e-6
Identity = 28/77 (36.36%), Postives = 43/77 (55.84%), Query Frame = 3
Query: 1521 CHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDR-IFDQLTSLDRHIR 1748
            C  C + FP   +L  H  +H+ E+ YKC  C K F+  SN+ RH  + H+  + Y+C  C + +F   ++L RHIR
Sbjct:   83 CKECEQVFPDTQSLEAHSLSHSEEREYKCDQCPKAFNWKSNLIRHQMS-HDSGKHYECENCSKQVFTDPSNLQRHIR 158          
BLAST of SMED30024013 vs. Ensembl Cavefish
Match: si:ch211-263k4.2 (PR/SET domain 16 [Source:NCBI gene;Acc:103028334])

HSP 1 Score: 129.798 bits (325), Expect = 6.654e-31
Identity = 55/80 (68.75%), Postives = 67/80 (83.75%), Query Frame = 3
Query: 1512 RYHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIRQ 1751
            RY C  C K FPR+ANL RHLRTHTGEQPY+C +CD+ FSISSN+QRHVRNIHNKE+P+KC  C+R F Q T+LDRH+++
Sbjct:  983 RYACRYCGKIFPRSANLTRHLRTHTGEQPYRCKYCDRSFSISSNLQRHVRNIHNKEKPFKCHLCNRCFGQQTNLDRHLKK 1062          

HSP 2 Score: 117.472 bits (293), Expect = 6.558e-27
Identity = 74/217 (34.10%), Postives = 117/217 (53.92%), Query Frame = 3
Query:  279 FQCPDCGANFSSRGPLKRHQNALCKNPNGLLIKTRQTIFKHNS-----VIQRK--QVLGTNQNCTTTNLSGGSEHRKTSSDY-KQSVSFPISKLSSPISNGSNLNLFCDVCNKPCRSQVSLIRH-RKQHQNPNSHYSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYTQYSNLCRHKRMSRDCQRKAKCTECGMEFTNGKTYDTHKQHCLG 902
            ++C DC   F+S+  L+RHQ   C + N +     + + +        V + K  + +  ++   + ++   +E R+   D   ++ ++  + +   +S+ S     C+ C+K      +L RH R QH    +H +C  CGKTF  SSGLKQH HIHSS KP+ C  C KSYTQ+SNLCRHKRM  DC+ + KC +CG  F+   + + H++ C G
Sbjct:  231 YRCEDCDELFTSKLELRRHQKYSCSSGNSIFDTLSEDLKQEREDSDEPVYECKDCEKIFPSEYSLSQHMVVHTEEREYKCDQCPKAFNWKSNLIRHQMSHDSGKRFECENCDKVFTDPSNLQRHIRSQHVGARAH-TCPECGKTFATSSGLKQHKHIHSSVKPFMCEVCHKSYTQFSNLCRHKRMHADCRTQIKCKDCGQMFSTTSSLNKHRRFCEG 446          

HSP 3 Score: 66.2402 bits (160), Expect = 7.025e-11
Identity = 31/78 (39.74%), Postives = 47/78 (60.26%), Query Frame = 3
Query: 1515 YHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIR 1748
            Y C  C K FP   +L++H+  HT E+ YKC  C K F+  SN+ RH  + H+  + ++C  CD++F   ++L RHIR
Sbjct:  280 YECKDCEKIFPSEYSLSQHMVVHTEEREYKCDQCPKAFNWKSNLIRHQMS-HDSGKRFECENCDKVFTDPSNLQRHIR 356          

HSP 4 Score: 60.077 bits (144), Expect = 5.854e-9
Identity = 29/80 (36.25%), Postives = 48/80 (60.00%), Query Frame = 3
Query: 1512 RYHCHLCLKTFPRAANLNRHLRT-HTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIR 1748
            R+ C  C K F   +NL RH+R+ H G + + C  C K F+ SS +++H ++IH+  +P+ C  C + + Q ++L RH R
Sbjct:  335 RFECENCDKVFTDPSNLQRHIRSQHVGARAHTCPECGKTFATSSGLKQH-KHIHSSVKPFMCEVCHKSYTQFSNLCRHKR 413          

HSP 5 Score: 60.077 bits (144), Expect = 5.955e-9
Identity = 28/77 (36.36%), Postives = 42/77 (54.55%), Query Frame = 3
Query: 1512 RYHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRH 1742
             Y C  C K F   +NL RH  +H   + ++C  CDK F+  SN+QRH+R+ H   R + C +C + F   + L +H
Sbjct:  307 EYKCDQCPKAFNWKSNLIRHQMSHDSGKRFECENCDKVFTDPSNLQRHIRSQHVGARAHTCPECGKTFATSSGLKQH 383          

HSP 6 Score: 56.225 bits (134), Expect = 8.750e-8
Identity = 24/56 (42.86%), Postives = 36/56 (64.29%), Query Frame = 3
Query: 1587 GEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIRQL 1754
            G++ Y C +C K F  S+N+ RH+R  H  E+PY+C  CDR F   ++L RH+R +
Sbjct:  980 GKERYACRYCGKIFPRSANLTRHLR-THTGEQPYRCKYCDRSFSISSNLQRHVRNI 1034          

HSP 7 Score: 53.9138 bits (128), Expect = 4.520e-7
Identity = 29/80 (36.25%), Postives = 42/80 (52.50%), Query Frame = 3
Query:  657 YSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYTQYSNLCRHKRMSRDCQRKAKCTECGMEFTNGKTYDTH-KQH 893
            Y+C  CGK F  S+ L +H+  H+  +PY C  C +S++  SNL RH R   + ++  KC  C   F      D H K+H
Sbjct:  984 YACRYCGKIFPRSANLTRHLRTHTGEQPYRCKYCDRSFSISSNLQRHVRNIHNKEKPFKCHLCNRCFGQQTNLDRHLKKH 1063          

HSP 8 Score: 50.447 bits (119), Expect = 5.297e-6
Identity = 31/93 (33.33%), Postives = 44/93 (47.31%), Query Frame = 3
Query:  522 PISKLSSPISNGSNLNLFCDVCNKPCRSQVSLIRHRKQHQNPNSHYSCDVCGKTFKISSGLKQHM-HIHSSNKPYSCLNCQKSYTQYSNLCRH 797
            P   LS  I         C  C K      +L RH + H      Y C  C ++F ISS L++H+ +IH+  KP+ C  C + + Q +NL RH
Sbjct:  968 PPPSLSDAILRKGKERYACRYCGKIFPRSANLTRHLRTHTG-EQPYRCKYCDRSFSISSNLQRHVRNIHNKEKPFKCHLCNRCFGQQTNLDRH 1059          
BLAST of SMED30024013 vs. Ensembl Cavefish
Match: si:ch211-263k4.2 (PR/SET domain 16 [Source:NCBI gene;Acc:103028334])

HSP 1 Score: 129.798 bits (325), Expect = 7.193e-31
Identity = 55/80 (68.75%), Postives = 67/80 (83.75%), Query Frame = 3
Query: 1512 RYHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIRQ 1751
            RY C  C K FPR+ANL RHLRTHTGEQPY+C +CD+ FSISSN+QRHVRNIHNKE+P+KC  C+R F Q T+LDRH+++
Sbjct:  975 RYACRYCGKIFPRSANLTRHLRTHTGEQPYRCKYCDRSFSISSNLQRHVRNIHNKEKPFKCHLCNRCFGQQTNLDRHLKK 1054          

HSP 2 Score: 117.472 bits (293), Expect = 6.501e-27
Identity = 74/217 (34.10%), Postives = 117/217 (53.92%), Query Frame = 3
Query:  279 FQCPDCGANFSSRGPLKRHQNALCKNPNGLLIKTRQTIFKHNS-----VIQRK--QVLGTNQNCTTTNLSGGSEHRKTSSDY-KQSVSFPISKLSSPISNGSNLNLFCDVCNKPCRSQVSLIRH-RKQHQNPNSHYSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYTQYSNLCRHKRMSRDCQRKAKCTECGMEFTNGKTYDTHKQHCLG 902
            ++C DC   F+S+  L+RHQ   C + N +     + + +        V + K  + +  ++   + ++   +E R+   D   ++ ++  + +   +S+ S     C+ C+K      +L RH R QH    +H +C  CGKTF  SSGLKQH HIHSS KP+ C  C KSYTQ+SNLCRHKRM  DC+ + KC +CG  F+   + + H++ C G
Sbjct:  223 YRCEDCDELFTSKLELRRHQKYSCSSGNSIFDTLSEDLKQEREDSDEPVYECKDCEKIFPSEYSLSQHMVVHTEEREYKCDQCPKAFNWKSNLIRHQMSHDSGKRFECENCDKVFTDPSNLQRHIRSQHVGARAH-TCPECGKTFATSSGLKQHKHIHSSVKPFMCEVCHKSYTQFSNLCRHKRMHADCRTQIKCKDCGQMFSTTSSLNKHRRFCEG 438          

HSP 3 Score: 66.2402 bits (160), Expect = 7.551e-11
Identity = 31/78 (39.74%), Postives = 47/78 (60.26%), Query Frame = 3
Query: 1515 YHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIR 1748
            Y C  C K FP   +L++H+  HT E+ YKC  C K F+  SN+ RH  + H+  + ++C  CD++F   ++L RHIR
Sbjct:  272 YECKDCEKIFPSEYSLSQHMVVHTEEREYKCDQCPKAFNWKSNLIRHQMS-HDSGKRFECENCDKVFTDPSNLQRHIR 348          

HSP 4 Score: 60.077 bits (144), Expect = 5.891e-9
Identity = 29/80 (36.25%), Postives = 48/80 (60.00%), Query Frame = 3
Query: 1512 RYHCHLCLKTFPRAANLNRHLRT-HTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIR 1748
            R+ C  C K F   +NL RH+R+ H G + + C  C K F+ SS +++H ++IH+  +P+ C  C + + Q ++L RH R
Sbjct:  327 RFECENCDKVFTDPSNLQRHIRSQHVGARAHTCPECGKTFATSSGLKQH-KHIHSSVKPFMCEVCHKSYTQFSNLCRHKR 405          

HSP 5 Score: 60.077 bits (144), Expect = 6.255e-9
Identity = 28/77 (36.36%), Postives = 42/77 (54.55%), Query Frame = 3
Query: 1512 RYHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRH 1742
             Y C  C K F   +NL RH  +H   + ++C  CDK F+  SN+QRH+R+ H   R + C +C + F   + L +H
Sbjct:  299 EYKCDQCPKAFNWKSNLIRHQMSHDSGKRFECENCDKVFTDPSNLQRHIRSQHVGARAHTCPECGKTFATSSGLKQH 375          

HSP 6 Score: 56.225 bits (134), Expect = 9.365e-8
Identity = 24/56 (42.86%), Postives = 36/56 (64.29%), Query Frame = 3
Query: 1587 GEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIRQL 1754
            G++ Y C +C K F  S+N+ RH+R  H  E+PY+C  CDR F   ++L RH+R +
Sbjct:  972 GKERYACRYCGKIFPRSANLTRHLR-THTGEQPYRCKYCDRSFSISSNLQRHVRNI 1026          

HSP 7 Score: 53.9138 bits (128), Expect = 4.842e-7
Identity = 29/80 (36.25%), Postives = 42/80 (52.50%), Query Frame = 3
Query:  657 YSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYTQYSNLCRHKRMSRDCQRKAKCTECGMEFTNGKTYDTH-KQH 893
            Y+C  CGK F  S+ L +H+  H+  +PY C  C +S++  SNL RH R   + ++  KC  C   F      D H K+H
Sbjct:  976 YACRYCGKIFPRSANLTRHLRTHTGEQPYRCKYCDRSFSISSNLQRHVRNIHNKEKPFKCHLCNRCFGQQTNLDRHLKKH 1055          

HSP 8 Score: 50.447 bits (119), Expect = 5.217e-6
Identity = 31/93 (33.33%), Postives = 44/93 (47.31%), Query Frame = 3
Query:  522 PISKLSSPISNGSNLNLFCDVCNKPCRSQVSLIRHRKQHQNPNSHYSCDVCGKTFKISSGLKQHM-HIHSSNKPYSCLNCQKSYTQYSNLCRH 797
            P   LS  I         C  C K      +L RH + H      Y C  C ++F ISS L++H+ +IH+  KP+ C  C + + Q +NL RH
Sbjct:  960 PPPSLSDAILRKGKERYACRYCGKIFPRSANLTRHLRTHTG-EQPYRCKYCDRSFSISSNLQRHVRNIHNKEKPFKCHLCNRCFGQQTNLDRH 1051          
BLAST of SMED30024013 vs. Ensembl Cavefish
Match: si:ch211-263k4.2 (PR/SET domain 16 [Source:NCBI gene;Acc:103028334])

HSP 1 Score: 129.413 bits (324), Expect = 7.689e-31
Identity = 55/80 (68.75%), Postives = 67/80 (83.75%), Query Frame = 3
Query: 1512 RYHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIRQ 1751
            RY C  C K FPR+ANL RHLRTHTGEQPY+C +CD+ FSISSN+QRHVRNIHNKE+P+KC  C+R F Q T+LDRH+++
Sbjct:  983 RYACRYCGKIFPRSANLTRHLRTHTGEQPYRCKYCDRSFSISSNLQRHVRNIHNKEKPFKCHLCNRCFGQQTNLDRHLKK 1062          

HSP 2 Score: 117.087 bits (292), Expect = 7.194e-27
Identity = 74/217 (34.10%), Postives = 117/217 (53.92%), Query Frame = 3
Query:  279 FQCPDCGANFSSRGPLKRHQNALCKNPNGLLIKTRQTIFKHNS-----VIQRK--QVLGTNQNCTTTNLSGGSEHRKTSSDY-KQSVSFPISKLSSPISNGSNLNLFCDVCNKPCRSQVSLIRH-RKQHQNPNSHYSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYTQYSNLCRHKRMSRDCQRKAKCTECGMEFTNGKTYDTHKQHCLG 902
            ++C DC   F+S+  L+RHQ   C + N +     + + +        V + K  + +  ++   + ++   +E R+   D   ++ ++  + +   +S+ S     C+ C+K      +L RH R QH    +H +C  CGKTF  SSGLKQH HIHSS KP+ C  C KSYTQ+SNLCRHKRM  DC+ + KC +CG  F+   + + H++ C G
Sbjct:  231 YRCEDCDELFTSKLELRRHQKYSCSSGNSIFDTLSEDLKQEREDSDEPVYECKDCEKIFPSEYSLSQHMVVHTEEREYKCDQCPKAFNWKSNLIRHQMSHDSGKRFECENCDKVFTDPSNLQRHIRSQHVGARAH-TCPECGKTFATSSGLKQHKHIHSSVKPFMCEVCHKSYTQFSNLCRHKRMHADCRTQIKCKDCGQMFSTTSSLNKHRRFCEG 446          

HSP 3 Score: 66.2402 bits (160), Expect = 7.829e-11
Identity = 31/78 (39.74%), Postives = 47/78 (60.26%), Query Frame = 3
Query: 1515 YHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIR 1748
            Y C  C K FP   +L++H+  HT E+ YKC  C K F+  SN+ RH  + H+  + ++C  CD++F   ++L RHIR
Sbjct:  280 YECKDCEKIFPSEYSLSQHMVVHTEEREYKCDQCPKAFNWKSNLIRHQMS-HDSGKRFECENCDKVFTDPSNLQRHIR 356          

HSP 4 Score: 60.077 bits (144), Expect = 6.212e-9
Identity = 29/80 (36.25%), Postives = 48/80 (60.00%), Query Frame = 3
Query: 1512 RYHCHLCLKTFPRAANLNRHLRT-HTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIR 1748
            R+ C  C K F   +NL RH+R+ H G + + C  C K F+ SS +++H ++IH+  +P+ C  C + + Q ++L RH R
Sbjct:  335 RFECENCDKVFTDPSNLQRHIRSQHVGARAHTCPECGKTFATSSGLKQH-KHIHSSVKPFMCEVCHKSYTQFSNLCRHKR 413          

HSP 5 Score: 60.077 bits (144), Expect = 6.373e-9
Identity = 28/77 (36.36%), Postives = 42/77 (54.55%), Query Frame = 3
Query: 1512 RYHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRH 1742
             Y C  C K F   +NL RH  +H   + ++C  CDK F+  SN+QRH+R+ H   R + C +C + F   + L +H
Sbjct:  307 EYKCDQCPKAFNWKSNLIRHQMSHDSGKRFECENCDKVFTDPSNLQRHIRSQHVGARAHTCPECGKTFATSSGLKQH 383          

HSP 6 Score: 56.225 bits (134), Expect = 9.379e-8
Identity = 24/56 (42.86%), Postives = 36/56 (64.29%), Query Frame = 3
Query: 1587 GEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIRQL 1754
            G++ Y C +C K F  S+N+ RH+R  H  E+PY+C  CDR F   ++L RH+R +
Sbjct:  980 GKERYACRYCGKIFPRSANLTRHLR-THTGEQPYRCKYCDRSFSISSNLQRHVRNI 1034          

HSP 7 Score: 53.9138 bits (128), Expect = 5.018e-7
Identity = 29/80 (36.25%), Postives = 42/80 (52.50%), Query Frame = 3
Query:  657 YSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYTQYSNLCRHKRMSRDCQRKAKCTECGMEFTNGKTYDTH-KQH 893
            Y+C  CGK F  S+ L +H+  H+  +PY C  C +S++  SNL RH R   + ++  KC  C   F      D H K+H
Sbjct:  984 YACRYCGKIFPRSANLTRHLRTHTGEQPYRCKYCDRSFSISSNLQRHVRNIHNKEKPFKCHLCNRCFGQQTNLDRHLKKH 1063          

HSP 8 Score: 50.447 bits (119), Expect = 5.179e-6
Identity = 31/93 (33.33%), Postives = 44/93 (47.31%), Query Frame = 3
Query:  522 PISKLSSPISNGSNLNLFCDVCNKPCRSQVSLIRHRKQHQNPNSHYSCDVCGKTFKISSGLKQHM-HIHSSNKPYSCLNCQKSYTQYSNLCRH 797
            P   LS  I         C  C K      +L RH + H      Y C  C ++F ISS L++H+ +IH+  KP+ C  C + + Q +NL RH
Sbjct:  968 PPPSLSDAILRKGKERYACRYCGKIFPRSANLTRHLRTHTG-EQPYRCKYCDRSFSISSNLQRHVRNIHNKEKPFKCHLCNRCFGQQTNLDRH 1059          
BLAST of SMED30024013 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000007831.1 (pep scaffold:Pmarinus_7.0:GL477655:47469:47963:1 gene:ENSPMAG00000007084.1 transcript:ENSPMAT00000007831.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 81.2629 bits (199), Expect = 6.470e-19
Identity = 35/76 (46.05%), Postives = 54/76 (71.05%), Query Frame = 3
Query: 1515 YHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRH 1742
            + C LC K F ++++L  H RTHTGE+P+KC+ C+K FS SSN+++H R  H  E+P+KCT C++ F + + L +H
Sbjct:   32 FRCPLCGKAFAKSSDLQSHQRTHTGEKPFKCSVCEKAFSQSSNLKKHQRT-HTGEKPFKCTVCEKAFTESSPLRKH 106          

HSP 2 Score: 68.5514 bits (166), Expect = 1.936e-14
Identity = 32/78 (41.03%), Postives = 51/78 (65.38%), Query Frame = 3
Query: 1515 YHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIR 1748
            + C +C K F +++ L  HLRTHT  +P++C  C K F+ SS++Q H R  H  E+P+KC+ C++ F Q ++L +H R
Sbjct:    6 FKCTVCEKAFAQSSYLTIHLRTHT--KPFRCPLCGKAFAKSSDLQSHQRT-HTGEKPFKCSVCEKAFSQSSNLKKHQR 80          

HSP 3 Score: 62.7734 bits (151), Expect = 1.816e-12
Identity = 36/104 (34.62%), Postives = 51/104 (49.04%), Query Frame = 3
Query:  576 CDVCNKPCRSQVSLIRHRKQHQNPNSHYSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYTQYSNLCRHKRMSRDCQRKAKCTECGMEFTNGKTYDTHK 887
            C VC K       L  H + H  P   + C +CGK F  SS L+ H   H+  KP+ C  C+K+++Q SNL +H+R     ++  KCT C   FT       H+
Sbjct:    8 CTVCEKAFAQSSYLTIHLRTHTKP---FRCPLCGKAFAKSSDLQSHQRTHTGEKPFKCSVCEKAFSQSSNLKKHQRTHTG-EKPFKCTVCEKAFTESSPLRKHQ 107          
BLAST of SMED30024013 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000000449.1 (pep scaffold:Pmarinus_7.0:GL478964:311851:312750:-1 gene:ENSPMAG00000000404.1 transcript:ENSPMAT00000000449.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 81.2629 bits (199), Expect = 1.296e-17
Identity = 36/78 (46.15%), Postives = 50/78 (64.10%), Query Frame = 3
Query: 1515 YHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIR 1748
            YHC +C K F +  NLN H RTHTGE+PY+C  C K F+  +N+  H R  H  E+P++C  CD+ F Q+ +L+ H R
Sbjct:  163 YHCKMCDKAFSQVTNLNNHYRTHTGEKPYRCQICGKAFTQVTNLNNHYR-THTGEKPFRCKMCDKAFSQVANLNSHQR 239          

HSP 2 Score: 73.559 bits (179), Expect = 6.101e-15
Identity = 35/76 (46.05%), Postives = 45/76 (59.21%), Query Frame = 3
Query: 1521 CHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIR 1748
            C  C K F +   LN H R HTGE+PY C  CDK FS  +N+  H R  H  E+PY+C  C + F Q+T+L+ H R
Sbjct:  137 CKECGKAFSQVTQLNNHWRIHTGEKPYHCKMCDKAFSQVTNLNNHYR-THTGEKPYRCQICGKAFTQVTNLNNHYR 211          

HSP 3 Score: 62.003 bits (149), Expect = 5.036e-11
Identity = 31/76 (40.79%), Postives = 43/76 (56.58%), Query Frame = 3
Query: 1521 CHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIR 1748
            C +C K F  A +LN H   H  ++P+ C  C K FS  + +  H R IH  E+PY C  CD+ F Q+T+L+ H R
Sbjct:  109 CKVCNKVFSHAKHLNNHQHIHMRDKPFICKECGKAFSQVTQLNNHWR-IHTGEKPYHCKMCDKAFSQVTNLNNHYR 183          

HSP 4 Score: 58.151 bits (139), Expect = 8.803e-10
Identity = 26/52 (50.00%), Postives = 33/52 (63.46%), Query Frame = 3
Query: 1515 YHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIH 1670
            Y C +C K F +  NLN H RTHTGE+P++C  CDK FS  +N+  H R  H
Sbjct:  191 YRCQICGKAFTQVTNLNNHYRTHTGEKPFRCKMCDKAFSQVANLNSHQRIHH 242          

HSP 5 Score: 56.9954 bits (136), Expect = 2.083e-9
Identity = 52/241 (21.58%), Postives = 89/241 (36.93%), Query Frame = 3
Query:  216 PTFIASVANIYSKSTTQASSQFQCPDCGANFSSRGPLKRHQNALCKNPNGLLIKTRQTIFKHNSVIQRKQVLGTNQNCTTTNLSGGSEHRKTSSD-----YKQSVSFPISKLSSPISNGSNLN-----------LFCDVCNKPCRSQVSLIRHRKQHQNPNSHYSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYTQYSNLCRHKRMSRDCQRKAKCTECGMEFTNGKTYDTHKQ 890
            P  +    + + K        + C  CG  F   G  KRH+    +  N       + +F+  S ++ +Q +   +NC      G +  + T  +     ++Q         +   S+  +LN             C  C K       L  H + H      Y C +C K F   + L  H   H+  KPY C  C K++TQ +NL  H R     ++  +C  C   F+     ++H++
Sbjct:    4 PYSVKLTGDAFKKHKALGERAYHCERCGRGFLQNGAFKRHRRMHTRG-NLYGCDFHERMFR--SYLKNEQRIHRGENCRRCEKCGEAFSKVTKRNSHQCIHRQETPHCCKVCNKVFSHAKHLNNHQHIHMRDKPFICKECGKAFSQVTQLNNHWRIHTGEKP-YHCKMCDKAFSQVTNLNNHYRTHTGEKPYRCQICGKAFTQVTNLNNHYRTHTG-EKPFRCKMCDKAFSQVANLNSHQR 239          

HSP 6 Score: 50.447 bits (119), Expect = 3.787e-7
Identity = 25/76 (32.89%), Postives = 42/76 (55.26%), Query Frame = 3
Query: 1521 CHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIR 1748
            C  C + F +    N H   H  E P+ C  C+K FS + ++  H ++IH +++P+ C +C + F Q+T L+ H R
Sbjct:   81 CEKCGEAFSKVTKRNSHQCIHRQETPHCCKVCNKVFSHAKHLNNH-QHIHMRDKPFICKECGKAFSQVTQLNNHWR 155          
BLAST of SMED30024013 vs. Ensembl Sea Lamprey
Match: ZNF436 (zinc finger protein 436 [Source:HGNC Symbol;Acc:HGNC:20814])

HSP 1 Score: 81.6481 bits (200), Expect = 3.169e-17
Identity = 37/77 (48.05%), Postives = 51/77 (66.23%), Query Frame = 3
Query: 1512 RYHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRH 1742
            R+ C  C + F   ++L +H RTHTGE+PY CA C + FS++S++ +H R +H  ERPY C  C R F QLTSL +H
Sbjct:    3 RHACPQCGRCFALRSSLTKHARTHTGERPYACAACGRSFSVASSLVKH-RRVHTGERPYACAACGRAFSQLTSLQKH 78          

HSP 2 Score: 61.2326 bits (147), Expect = 1.894e-10
Identity = 30/76 (39.47%), Postives = 43/76 (56.58%), Query Frame = 3
Query: 1521 CHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIR 1748
            C  C + F     L +H RTHTGE+P+ CA C + F+  S +++H R  H  ERP+ C +C R F   ++L  H R
Sbjct:  211 CSECGRRFLSVRELAQHARTHTGERPHVCAECGRAFAAVSTLRKHAR-THTGERPHVCPQCGRAFTSSSNLRAHAR 285          

HSP 3 Score: 60.077 bits (144), Expect = 4.305e-10
Identity = 52/210 (24.76%), Postives = 86/210 (40.95%), Query Frame = 3
Query:  285 CPDCGANFSSRGPLKRHQNALCKNPNGLLIKTR-------QTIFKHNSVIQRKQVLGTNQNCTTTNLSGGSEHRKTSSDYKQSVSFPISKLSSPISNGSNLNLFCDVCNKPCRSQVSLIRHRKQH-QNPNSHYSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYTQYSNLCRHKRMSRDCQRKAKCTECGMEFTNGKTYDTHKQ 890
            C  CG  FS R  L++H+    +   GL+ + R        T F  N   QR Q   T +      L G +    ++    QS            ++  +    C++C K      +L +H K+H ++ N  ++C  CG+ F     L QH   H+  +P+ C  C +++   S L +H R     +R   C +CG  FT+      H +
Sbjct:   90 CATCGKAFSHRAFLRKHEKTHAERVAGLVKERRFACERCCATFFD-NQSRQRHQTTHTGERPFVCELCGKAFSHHSTLRKHQS------------THSGDRPHVCELCGKAFPHATNLKQHLKRHTEDKNKPHACSECGRRFLSVRELAQHARTHTGERPHVCAECGRAFAAVSTLRKHARTHTG-ERPHVCPQCGRAFTSSSNLRAHAR 285          

HSP 4 Score: 58.9214 bits (141), Expect = 1.129e-9
Identity = 27/77 (35.06%), Postives = 47/77 (61.04%), Query Frame = 3
Query: 1521 CHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHV-RNIHNKERPYKCTKCDRIFDQLTSLDRHIR 1748
            C LC K F   + L +H  TH+G++P+ C  C K F  ++N+++H+ R+  +K +P+ C++C R F  +  L +H R
Sbjct:  153 CELCGKAFSHHSTLRKHQSTHSGDRPHVCELCGKAFPHATNLKQHLKRHTEDKNKPHACSECGRRFLSVRELAQHAR 229          

HSP 5 Score: 58.151 bits (139), Expect = 1.770e-9
Identity = 29/80 (36.25%), Postives = 47/80 (58.75%), Query Frame = 3
Query: 1512 RYHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIRQ 1751
            R+ C  C  TF    +  RH  THTGE+P+ C  C K FS  S +++H ++ H+ +RP+ C  C + F   T+L +H+++
Sbjct:  122 RFACERCCATFFDNQSRQRHQTTHTGERPFVCELCGKAFSHHSTLRKH-QSTHSGDRPHVCELCGKAFPHATNLKQHLKR 200          

HSP 6 Score: 56.9954 bits (136), Expect = 4.231e-9
Identity = 28/75 (37.33%), Postives = 43/75 (57.33%), Query Frame = 3
Query: 1521 CHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERP-YKCTKCDRIFDQLTSLDRH 1742
            C  C + F   + L +H RTHTGE+P+ C  C + F+ SSN++ H R    + RP + CT+C   F +  +L +H
Sbjct:  239 CAECGRAFAAVSTLRKHARTHTGERPHVCPQCGRAFTSSSNLRAHARAHAGQRRPQHACTQCGCRFARAGALRKH 313          

HSP 7 Score: 56.6102 bits (135), Expect = 4.973e-9
Identity = 51/215 (23.72%), Postives = 89/215 (41.40%), Query Frame = 3
Query:  279 FQCPDCGANFSSRGPLKRHQNA-LCKNPNGLLIKTRQTIFKHNSVIQRKQVLGTNQNCTTTNLSGGSEHRKTSSDYKQSVSFP-ISKLSSPISNGSNLNLFCDVCNKPCRSQVSLIRHRKQHQNPNSHYSCDVCGKTFKISSGLKQHM--HIHSSNKPYSCLNCQKSYTQYSNLCRHKRMSRDCQRKAKCTECGMEFTNGKTYDTHKQHCLGLDP 911
            + C  CG  FS    L++HQ     + P+     T    F H + +++      ++      ++G  + R+ + +   +  F   S+     ++       C++C K      +L +H+  H     H  C++CGK F  ++ LKQH+  H    NKP++C  C + +     L +H R     +R   C ECG  F    T   H +   G  P
Sbjct:   60 YACAACGRAFSQLTSLQKHQGRHSAEKPHACA--TCGKAFSHRAFLRK------HEKTHAERVAGLVKERRFACERCCATFFDNQSRQRHQTTHTGERPFVCELCGKAFSHHSTLRKHQSTHSGDRPHV-CELCGKAFPHATNLKQHLKRHTEDKNKPHACSECGRRFLSVRELAQHARTHTG-ERPHVCAECGRAFAAVSTLRKHARTHTGERP 264          

HSP 8 Score: 55.8398 bits (133), Expect = 1.056e-8
Identity = 31/114 (27.19%), Postives = 58/114 (50.88%), Query Frame = 3
Query:  576 CDVCNKPCRSQVSLIRHRKQHQNPNSHYSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYTQYSNLCRHKRMSRDCQRKAKCTECGMEFTN----GKTYDTHKQHCLGL 905
            C  C +    + SL +H + H      Y+C  CG++F ++S L +H  +H+  +PY+C  C ++++Q ++L +H+      ++   C  CG  F++     K   TH +   GL
Sbjct:    6 CPQCGRCFALRSSLTKHARTHTGERP-YACAACGRSFSVASSLVKHRRVHTGERPYACAACGRAFSQLTSLQKHQ-GRHSAEKPHACATCGKAFSHRAFLRKHEKTHAERVAGL 117          

HSP 9 Score: 51.9878 bits (123), Expect = 1.629e-7
Identity = 23/77 (29.87%), Postives = 42/77 (54.55%), Query Frame = 3
Query:  657 YSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYTQYSNLCRHKRMSRDCQRKAKCTECGMEFTNGKTYDTHK 887
            ++C  CG+ F + S L +H   H+  +PY+C  C +S++  S+L +H+R+    +R   C  CG  F+   +   H+
Sbjct:    4 HACPQCGRCFALRSSLTKHARTHTGERPYACAACGRSFSVASSLVKHRRVHTG-ERPYACAACGRAFSQLTSLQKHQ 79          

HSP 10 Score: 47.3654 bits (111), Expect = 5.550e-6
Identity = 21/53 (39.62%), Postives = 31/53 (58.49%), Query Frame = 3
Query: 1590 EQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIR 1748
            E+ + C  C +CF++ S++ +H R  H  ERPY C  C R F   +SL +H R
Sbjct:    1 EKRHACPQCGRCFALRSSLTKHAR-THTGERPYACAACGRSFSVASSLVKHRR 52          
BLAST of SMED30024013 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000007400.1 (pep scaffold:Pmarinus_7.0:GL476367:705224:706108:-1 gene:ENSPMAG00000006685.1 transcript:ENSPMAT00000007400.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 75.0998 bits (183), Expect = 5.348e-17
Identity = 31/50 (62.00%), Postives = 42/50 (84.00%), Query Frame = 3
Query: 1602 KCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIRQ 1751
            +C +CD+ FSISSN+QRHVRNIHNKE+P++C  C R F Q T+LDRH+++
Sbjct:    1 RCKYCDRSFSISSNLQRHVRNIHNKEKPFRCPLCSRCFGQQTNLDRHLKK 50          

HSP 2 Score: 46.9802 bits (110), Expect = 4.515e-7
Identity = 18/48 (37.50%), Postives = 31/48 (64.58%), Query Frame = 3
Query: 1521 CHLCLKTFPRAANLNRHLR-THTGEQPYKCAFCDKCFSISSNMQRHVR 1661
            C  C ++F  ++NL RH+R  H  E+P++C  C +CF   +N+ RH++
Sbjct:    2 CKYCDRSFSISSNLQRHVRNIHNKEKPFRCPLCSRCFGQQTNLDRHLK 49          
BLAST of SMED30024013 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000000923.1 (pep scaffold:Pmarinus_7.0:GL480775:18221:19434:-1 gene:ENSPMAG00000000839.1 transcript:ENSPMAT00000000923.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 72.0182 bits (175), Expect = 9.003e-16
Identity = 36/78 (46.15%), Postives = 44/78 (56.41%), Query Frame = 3
Query: 1515 YHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIR 1748
            Y C  C + F R AN  RH R HTGE+PY CA C + F    ++  H R +H  ERPY C  C R F +  SL RH+R
Sbjct:   22 YACGDCGRRFARGANEERHRRVHTGERPYACAACGRRFRQKPHLAAHTR-LHTGERPYACAACGRRFAKPYSLTRHLR 98          

HSP 2 Score: 58.5362 bits (140), Expect = 4.948e-11
Identity = 29/66 (43.94%), Postives = 37/66 (56.06%), Query Frame = 3
Query: 1551 AANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIR 1748
            A  L  ++R HTGE+PY C  C + F+  +N +RH R +H  ERPY C  C R F Q   L  H R
Sbjct:    6 AVCLGSNIRNHTGERPYACGDCGRRFARGANEERH-RRVHTGERPYACAACGRRFRQKPHLAAHTR 70          

HSP 3 Score: 47.7506 bits (112), Expect = 3.532e-7
Identity = 23/83 (27.71%), Postives = 39/83 (46.99%), Query Frame = 3
Query:  576 CDVCNKPCRSQVSLIRHRKQHQNPNSHYSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYTQYSNLCRHKRMSRDCQR 824
            C  C +      +  RHR+ H      Y+C  CG+ F+    L  H  +H+  +PY+C  C + + +  +L RH R +   +R
Sbjct:   24 CGDCGRRFARGANEERHRRVHTGERP-YACAACGRRFRQKPHLAAHTRLHTGERPYACAACGRRFAKPYSLTRHLRSAAHARR 105          

HSP 4 Score: 47.3654 bits (111), Expect = 4.208e-7
Identity = 21/76 (27.63%), Postives = 36/76 (47.37%), Query Frame = 3
Query:  657 YSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYTQYSNLCRHKRMSRDCQRKAKCTECGMEFTNGKTYDTH 884
            Y+C  CG+ F   +  ++H  +H+  +PY+C  C + + Q  +L  H R+    +R   C  CG  F    +   H
Sbjct:   22 YACGDCGRRFARGANEERHRRVHTGERPYACAACGRRFRQKPHLAAHTRLHTG-ERPYACAACGRRFAKPYSLTRH 96          

HSP 5 Score: 46.2098 bits (108), Expect = 9.810e-7
Identity = 21/56 (37.50%), Postives = 32/56 (57.14%), Query Frame = 3
Query: 1515 YHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKER 1682
            Y C  C + F +  +L  H R HTGE+PY CA C + F+   ++ RH+R+  +  R
Sbjct:   50 YACAACGRRFRQKPHLAAHTRLHTGERPYACAACGRRFAKPYSLTRHLRSAAHARR 105          
BLAST of SMED30024013 vs. Ensembl Yeast
Match: ZAP1 (Zinc-regulated transcription factor; binds to zinc-responsive promoters to induce transcription of certain genes in presence of zinc, represses other genes in low zinc; regulates its own transcription; contains seven zinc-finger domains [Source:SGD;Acc:S000003592])

HSP 1 Score: 71.2478 bits (173), Expect = 2.418e-13
Identity = 32/78 (41.03%), Postives = 51/78 (65.38%), Query Frame = 3
Query: 1515 YHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIR 1748
            Y C  C + F     L +H RTH+GE+PYKC  C+K F+ISS+++ H+R  H  E+P +C  C + F++ ++L +HI+
Sbjct:  768 YKCKTCKRCFSSEETLVQHTRTHSGEKPYKCHICNKKFAISSSLKIHIR-THTGEKPLQCKICGKRFNESSNLSKHIK 844          

HSP 2 Score: 68.9366 bits (167), Expect = 1.113e-12
Identity = 35/81 (43.21%), Postives = 48/81 (59.26%), Query Frame = 3
Query: 1512 RYHC--HLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIR 1748
             Y C  H C +TFP+   L RHL+ H+  +PYKC  C +CFS    + +H R  H+ E+PYKC  C++ F   +SL  HIR
Sbjct:  737 EYQCLWHDCHRTFPQRQKLIRHLKVHSKYKPYKCKTCKRCFSSEETLVQHTR-THSGEKPYKCHICNKKFAISSSLKIHIR 816          

HSP 3 Score: 68.5514 bits (166), Expect = 1.518e-12
Identity = 31/74 (41.89%), Postives = 48/74 (64.86%), Query Frame = 3
Query: 1515 YHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLD 1736
            Y CH+C K F  +++L  H+RTHTGE+P +C  C K F+ SSN+ +H++     ++ YKC+ C + FD L  L+
Sbjct:  796 YKCHICNKKFAISSSLKIHIRTHTGEKPLQCKICGKRFNESSNLSKHIK---THQKKYKCSDCSKSFDDLGKLN 866          

HSP 4 Score: 63.5438 bits (153), Expect = 6.377e-11
Identity = 38/116 (32.76%), Postives = 58/116 (50.00%), Query Frame = 3
Query:  576 CDVCNKPCRSQVSLIRHRKQHQNPNSHYSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYTQYSNLCRHKRMSRDCQRKAKCTECGMEFTNGKTYDTHKQHCLGLDPSGYL 923
            C  C +   S+ +L++H + H      Y C +C K F ISS LK H+  H+  KP  C  C K + + SNL +H +     Q+K KC++C   F +    ++ K  C  L+   YL
Sbjct:  770 CKTCKRCFSSEETLVQHTRTHSGEKP-YKCHICNKKFAISSSLKIHIRTHTGEKPLQCKICGKRFNESSNLSKHIKTH---QKKYKCSDCSKSFDDLGKLNSQKVKC-ALERKPYL 880          
BLAST of SMED30024013 vs. Ensembl Yeast
Match: AZF1 (Zinc-finger transcription factor; involved in diauxic shift; in the presence of glucose, activates transcription of genes involved in growth and carbon metabolism; in nonfermentable carbon sources, activates transcription of genes involved in maintenance of cell wall integrity; relocalizes to the cytosol in response to hypoxia [Source:SGD;Acc:S000005639])

HSP 1 Score: 64.3142 bits (155), Expect = 3.279e-11
Identity = 27/78 (34.62%), Postives = 46/78 (58.97%), Query Frame = 3
Query: 1512 RYHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHI 1745
            ++ C  C + F +A +L  H+R+H G +P+ C +C K F+   N++ H R +H  E+PY C  CD+ F +  +L  H+
Sbjct:  592 KHECPYCHRLFSQATHLEVHVRSHIGYKPFVCDYCGKRFTQGGNLRTHER-LHTGEKPYSCDICDKKFSRKGNLAAHL 668          

HSP 2 Score: 63.929 bits (154), Expect = 4.355e-11
Identity = 31/78 (39.74%), Postives = 42/78 (53.85%), Query Frame = 3
Query: 1515 YHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKC--TKCDRIFDQLTSLDRH 1742
            + C  C K F +  NL  H R HTGE+PY C  CDK FS   N+  H+   H K +P+ C    C++ F QL ++  H
Sbjct:  621 FVCDYCGKRFTQGGNLRTHERLHTGEKPYSCDICDKKFSRKGNLAAHLV-THQKLKPFVCKLENCNKTFTQLGNMKAH 697          

HSP 3 Score: 49.2914 bits (116), Expect = 1.660e-6
Identity = 27/79 (34.18%), Postives = 36/79 (45.57%), Query Frame = 3
Query:  570 LFCDVCNKPCRSQVSLIRHRKQHQNPNSHYSCDVCGKTFKISSGLKQHMHIHSSNKPYSC--LNCQKSYTQYSNLCRHK 800
              CD C K      +L  H + H      YSCD+C K F     L  H+  H   KP+ C   NC K++TQ  N+  H+
Sbjct:  621 FVCDYCGKRFTQGGNLRTHERLHTG-EKPYSCDICDKKFSRKGNLAAHLVTHQKLKPFVCKLENCNKTFTQLGNMKAHQ 698          
BLAST of SMED30024013 vs. Ensembl Yeast
Match: MSN2 (Stress-responsive transcriptional activator; activated in stochastic pulses of nuclear localization in response to various stress conditions; binds DNA at stress response elements of responsive genes; relative distribution to nucleus increases upon DNA replication stress [Source:SGD;Acc:S000004640])

HSP 1 Score: 59.6918 bits (143), Expect = 7.668e-10
Identity = 23/50 (46.00%), Postives = 37/50 (74.00%), Query Frame = 3
Query: 1515 YHCHLCLKTFPRAANLNRHLRT-HTGEQPYKCAFCDKCFSISSNMQRHVR 1661
            +HCH+C K+F R+ +L RH+R+ H+ E+P+ C  CDK FS S N+ +H++
Sbjct:  647 FHCHICPKSFKRSEHLKRHVRSVHSNERPFACHICDKKFSRSDNLSQHIK 696          

HSP 2 Score: 56.6102 bits (135), Expect = 7.998e-9
Identity = 22/53 (41.51%), Postives = 36/53 (67.92%), Query Frame = 3
Query: 1590 EQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIR 1748
            E+P+ C  C K F  S +++RHVR++H+ ERP+ C  CD+ F +  +L +HI+
Sbjct:  644 EKPFHCHICPKSFKRSEHLKRHVRSVHSNERPFACHICDKKFSRSDNLSQHIK 696          
BLAST of SMED30024013 vs. Ensembl Yeast
Match: CRZ1 (Transcription factor, activates transcription of stress response genes; nuclear localization is positively regulated by calcineurin-mediated dephosphorylation; rapidly localizes to the nucleus under blue light stress; can be activated in stochastic pulses of nuclear localization in response to calcium [Source:SGD;Acc:S000004972])

HSP 1 Score: 57.7658 bits (138), Expect = 3.618e-9
Identity = 29/87 (33.33%), Postives = 45/87 (51.72%), Query Frame = 3
Query: 1515 YHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCT---------KCDRIFDQLTSLDRHIR 1748
            + C +C K F R  NL  HLRTHT E+P+ C+ C K F+   + +RH  ++H  ++ Y C           C + F +  +L RH +
Sbjct:  569 FACDVCGKKFTRPYNLKSHLRTHTNERPFICSICGKAFARQHDRKRH-EDLHTGKKRYVCGGKLKDGKPWGCGKKFARSDALGRHFK 654          

HSP 2 Score: 48.9062 bits (115), Expect = 2.030e-6
Identity = 25/79 (31.65%), Postives = 43/79 (54.43%), Query Frame = 3
Query:  639 QNPNSHYSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYTQYSNLCRHKRMSRDCQRKAKCTECGMEFTNGKTY 875
            +NP ++++CDVCGK F     LK H+  H++ +P+ C  C K++ +  +  RH+    D     K   CG +  +GK +
Sbjct:  564 KNP-ANFACDVCGKKFTRPYNLKSHLRTHTNERPFICSICGKAFARQHDRKRHE----DLHTGKKRYVCGGKLKDGKPW 637          
BLAST of SMED30024013 vs. Ensembl Yeast
Match: ADR1 (Carbon source-responsive zinc-finger transcription factor; required for transcription of the glucose-repressed gene ADH2, of peroxisomal protein genes, and of genes required for ethanol, glycerol, and fatty acid utilization [Source:SGD;Acc:S000002624])

HSP 1 Score: 56.9954 bits (136), Expect = 7.862e-9
Identity = 21/52 (40.38%), Postives = 32/52 (61.54%), Query Frame = 3
Query: 1515 YHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIH 1670
            + C +C + F R  +L RH R+HT E+PY C  C++CF+    + RH + IH
Sbjct:  104 FVCEVCTRAFARQEHLKRHYRSHTNEKPYPCGLCNRCFTRRDLLIRHAQKIH 155          
BLAST of SMED30024013 vs. Ensembl Nematostella
Match: EDO47692 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RLH7])

HSP 1 Score: 83.5741 bits (205), Expect = 3.578e-19
Identity = 37/78 (47.44%), Postives = 54/78 (69.23%), Query Frame = 3
Query: 1515 YHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIR 1748
            Y C +CLK+F +AANL  H R H+GE+P++C  C + FS SS++  H+R  HN ERPYKC  C++ F   ++L +H+R
Sbjct:   22 YQCMVCLKSFTQAANLTAHFRIHSGEKPFRCRVCGRQFSQSSSVTTHMRT-HNGERPYKCKVCNKAFADSSTLTKHVR 98          

HSP 2 Score: 73.559 bits (179), Expect = 1.053e-15
Identity = 32/78 (41.03%), Postives = 52/78 (66.67%), Query Frame = 3
Query: 1515 YHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIR 1748
            + C +C + F +++++  H+RTH GE+PYKC  C+K F+ SS + +HVR  H  E+PY+C  C + F Q  +++RH R
Sbjct:   50 FRCRVCGRQFSQSSSVTTHMRTHNGERPYKCKVCNKAFADSSTLTKHVRT-HTGEKPYQCEICHQRFSQSGNMNRHKR 126          

HSP 3 Score: 64.3142 bits (155), Expect = 1.462e-12
Identity = 28/70 (40.00%), Postives = 46/70 (65.71%), Query Frame = 3
Query: 1539 TFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIR 1748
            ++ R + L  H+RTH+G +PY+C  C K F+ ++N+  H R IH+ E+P++C  C R F Q +S+  H+R
Sbjct:    2 SYARLSTLRLHMRTHSGVKPYQCMVCLKSFTQAANLTAHFR-IHSGEKPFRCRVCGRQFSQSSSVTTHMR 70          

HSP 4 Score: 58.9214 bits (141), Expect = 1.354e-10
Identity = 28/54 (51.85%), Postives = 37/54 (68.52%), Query Frame = 3
Query: 1515 YHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNK 1676
            Y C +C K F  ++ L +H+RTHTGE+PY+C  C + FS S NM RH R IHN+
Sbjct:   78 YKCKVCNKAFADSSTLTKHVRTHTGEKPYQCEICHQRFSQSGNMNRHKR-IHNE 130          

HSP 5 Score: 55.0694 bits (131), Expect = 3.076e-9
Identity = 29/77 (37.66%), Postives = 40/77 (51.95%), Query Frame = 3
Query:  576 CDVCNKPCRSQVSLIRHRKQHQNPNSHYSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYTQYSNLCRHKRM 806
            C VC +      S+  H + H N    Y C VC K F  SS L +H+  H+  KPY C  C + ++Q  N+ RHKR+
Sbjct:   52 CRVCGRQFSQSSSVTTHMRTH-NGERPYKCKVCNKAFADSSTLTKHVRTHTGEKPYQCEICHQRFSQSGNMNRHKRI 127          

HSP 6 Score: 50.8322 bits (120), Expect = 8.130e-8
Identity = 31/105 (29.52%), Postives = 48/105 (45.71%), Query Frame = 3
Query:  576 CDVCNKPCRSQVSLIRHRKQHQNPNSHYSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYTQYSNLCRHKRMSRDCQRKAKCTECGMEFTNGKTYDTHKQ 890
            C VC K      +L  H + H      + C VCG+ F  SS +  HM  H+  +PY C  C K++   S L +H R +   ++  +C  C   F+     + HK+
Sbjct:   24 CMVCLKSFTQAANLTAHFRIHSGEKP-FRCRVCGRQFSQSSSVTTHMRTHNGERPYKCKVCNKAFADSSTLTKHVR-THTGEKPYQCEICHQRFSQSGNMNRHKR 126          

HSP 7 Score: 50.0618 bits (118), Expect = 1.356e-7
Identity = 28/85 (32.94%), Postives = 42/85 (49.41%), Query Frame = 3
Query:  657 YSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYTQYSNLCRHKRMSRDCQRKAKCTECGMEFTNGKTYDTHKQHCLGLDP 911
            Y C VC K+F  ++ L  H  IHS  KP+ C  C + ++Q S++  H R + + +R  KC  C   F +  T   H +   G  P
Sbjct:   22 YQCMVCLKSFTQAANLTAHFRIHSGEKPFRCRVCGRQFSQSSSVTTHMR-THNGERPYKCKVCNKAFADSSTLTKHVRTHTGEKP 105          
BLAST of SMED30024013 vs. Ensembl Nematostella
Match: EDO32891 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7STS3])

HSP 1 Score: 85.1149 bits (209), Expect = 1.144e-18
Identity = 36/77 (46.75%), Postives = 55/77 (71.43%), Query Frame = 3
Query: 1521 CHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIRQ 1751
            CH+C K F ++++LN+H+R H+GE+PYKC FCDK F+ SS ++ H+R  H+ E+P+KC  C R F    + D H+R+
Sbjct:  130 CHVCQKRFSQSSSLNKHMRVHSGERPYKCTFCDKAFTASSILRTHIRQ-HSGEKPFKCKYCGRAFASHAAHDSHVRR 205          

HSP 2 Score: 63.5438 bits (153), Expect = 2.395e-11
Identity = 26/76 (34.21%), Postives = 43/76 (56.58%), Query Frame = 3
Query: 1515 YHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRH 1742
            Y C  C K F  ++ L  H+R H+GE+P+KC +C + F+  +    HVR  H +++ + C  C ++F  +T L  H
Sbjct:  156 YKCTFCDKAFTASSILRTHIRQHSGEKPFKCKYCGRAFASHAAHDSHVRRTHTRDQTWTCEVCGQVFTDMTQLHYH 231          

HSP 3 Score: 55.0694 bits (131), Expect = 1.549e-8
Identity = 36/118 (30.51%), Postives = 55/118 (46.61%), Query Frame = 3
Query:  531 KLSSPISNGSNLNLFCDVCNKPCRSQVSLIRHRKQHQNPNSHYSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYTQYSNLCRHKRMSRDCQRKAKCTECGMEFTNGKTYDTH 884
            K +  + NG +    C +C++    +  L  H       +  + C VC K F  SS L +HM +HS  +PY C  C K++T  S L  H R     ++  KC  CG  F +   +D+H
Sbjct:   87 KYTRCLDNG-DRRFPCPLCDRSFDKRDRLRIHVLHVHEKHRPHECHVCQKRFSQSSSLNKHMRVHSGERPYKCTFCDKAFTASSILRTHIRQHSG-EKPFKCKYCGRAFASHAAHDSH 202          

HSP 4 Score: 51.2174 bits (121), Expect = 3.324e-7
Identity = 25/80 (31.25%), Postives = 43/80 (53.75%), Query Frame = 3
Query: 1521 CHLCLKTFPRAANLNRHLR----THTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIR 1748
            C  C K F      ++HL+       G++ + C  CD+ F     ++ HV ++H K RP++C  C + F Q +SL++H+R
Sbjct:   69 CERCGKLFAYEYYRDKHLKYTRCLDNGDRRFPCPLCDRSFDKRDRLRIHVLHVHEKHRPHECHVCQKRFSQSSSLNKHMR 148          

HSP 5 Score: 50.447 bits (119), Expect = 5.122e-7
Identity = 30/96 (31.25%), Postives = 43/96 (44.79%), Query Frame = 3
Query:  576 CDVCNKPCRSQVSLIRHRKQHQNPNSHYSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYTQYSNLCRHKRMSRDCQRKAKCTECGMEFTN 863
            C VC K      SL +H + H      Y C  C K F  SS L+ H+  HS  KP+ C  C +++  ++    H R +    +   C  CG  FT+
Sbjct:  130 CHVCQKRFSQSSSLNKHMRVHSGERP-YKCTFCDKAFTASSILRTHIRQHSGEKPFKCKYCGRAFASHAAHDSHVRRTHTRDQTWTCEVCGQVFTD 224          
BLAST of SMED30024013 vs. Ensembl Nematostella
Match: EDO45259 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RTG0])

HSP 1 Score: 82.4185 bits (202), Expect = 1.149e-18
Identity = 38/78 (48.72%), Postives = 50/78 (64.10%), Query Frame = 3
Query: 1515 YHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIR 1748
            + C  C K F     L RH+R+HTGE PYKC  C + F  S+++QRH+RN H  E+PYKC +C R F + T L RH+R
Sbjct:   35 WKCSFCSKAFQYPCYLRRHMRSHTGESPYKCTQCSRAFVRSTDLQRHLRN-HTGEKPYKCKECSRAFARSTDLKRHMR 111          

HSP 2 Score: 79.337 bits (194), Expect = 1.410e-17
Identity = 36/77 (46.75%), Postives = 51/77 (66.23%), Query Frame = 3
Query: 1515 YHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHI 1745
            Y C  C + F R+ +L RHLR HTGE+PYKC  C + F+ S++++RH+R  H  E+PYKC +C + F Q  SL  H+
Sbjct:   63 YKCTQCSRAFVRSTDLQRHLRNHTGEKPYKCKECSRAFARSTDLKRHMRT-HTGEKPYKCWQCSKAFSQSGSLQTHL 138          

HSP 3 Score: 53.5286 bits (127), Expect = 1.364e-8
Identity = 31/103 (30.10%), Postives = 51/103 (49.51%), Query Frame = 3
Query:  576 CDVCNKPCRSQVSLIRHRKQHQNPNSHYSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYTQYSNLCRHKRMSRDCQRKAKCTECGMEFTNGKTYDTH 884
            C  C+K  +    L RH + H    S Y C  C + F  S+ L++H+  H+  KPY C  C +++ + ++L RH R     ++  KC +C   F+   +  TH
Sbjct:   37 CSFCSKAFQYPCYLRRHMRSHTG-ESPYKCTQCSRAFVRSTDLQRHLRNHTGEKPYKCKECSRAFARSTDLKRHMRTHTG-EKPYKCWQCSKAFSQSGSLQTH 137          
BLAST of SMED30024013 vs. Ensembl Nematostella
Match: EDO49116 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RGU6])

HSP 1 Score: 81.2629 bits (199), Expect = 4.248e-18
Identity = 38/77 (49.35%), Postives = 52/77 (67.53%), Query Frame = 3
Query: 1515 YHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHI 1745
            Y C++C + F +  NL RH+ THTGE+P+KC +C K F  SS+++RHVR IH  ERPYKC +C + F    +L  HI
Sbjct:   60 YTCNICSRGFAQLTNLQRHILTHTGEKPHKCTYCPKAFVSSSDLRRHVR-IHTGERPYKCKQCSKAFTTSGNLQSHI 135          

HSP 2 Score: 77.7962 bits (190), Expect = 9.013e-17
Identity = 37/77 (48.05%), Postives = 50/77 (64.94%), Query Frame = 3
Query: 1515 YHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHI 1745
            Y C  C K F R+ N  RH+  HTG++PYKC  C K F+++++++ H R IH  E+PY C  C R F QLT+L RHI
Sbjct:    4 YTCQHCSKVFTRSWNFQRHVLIHTGQKPYKCQKCPKAFALAAHLKIHNR-IHTGEKPYTCNICSRGFAQLTNLQRHI 79          

HSP 3 Score: 72.0182 bits (175), Expect = 7.193e-15
Identity = 35/76 (46.05%), Postives = 48/76 (63.16%), Query Frame = 3
Query: 1521 CHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIR 1748
            C  C K F  +++L RH+R HTGE+PYKC  C K F+ S N+Q H+   H  E+PYKC+ C+  F   ++L  HIR
Sbjct:   90 CTYCPKAFVSSSDLRRHVRIHTGERPYKCKQCSKAFTTSGNLQSHILT-HTGEKPYKCSICNWKFISSSNLRTHIR 164          

HSP 4 Score: 71.633 bits (174), Expect = 1.047e-14
Identity = 35/78 (44.87%), Postives = 47/78 (60.26%), Query Frame = 3
Query: 1515 YHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIR 1748
            Y C  C K F  AA+L  H R HTGE+PY C  C + F+  +N+QRH+   H  E+P+KCT C + F   + L RH+R
Sbjct:   32 YKCQKCPKAFALAAHLKIHNRIHTGEKPYTCNICSRGFAQLTNLQRHILT-HTGEKPHKCTYCPKAFVSSSDLRRHVR 108          

HSP 5 Score: 54.299 bits (129), Expect = 1.105e-8
Identity = 32/103 (31.07%), Postives = 51/103 (49.51%), Query Frame = 3
Query:  576 CDVCNKPCRSQVSLIRHRKQHQNPNSHYSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYTQYSNLCRHKRMSRDCQRKAKCTECGMEFTNGKTYDTH 884
            C++C++      +L RH   H     H  C  C K F  SS L++H+ IH+  +PY C  C K++T   NL  H  ++   ++  KC+ C  +F +     TH
Sbjct:   62 CNICSRGFAQLTNLQRHILTHTGEKPH-KCTYCPKAFVSSSDLRRHVRIHTGERPYKCKQCSKAFTTSGNLQSHI-LTHTGEKPYKCSICNWKFISSSNLRTH 162          

HSP 6 Score: 52.7582 bits (125), Expect = 3.965e-8
Identity = 24/49 (48.98%), Postives = 32/49 (65.31%), Query Frame = 3
Query: 1515 YHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVR 1661
            Y C  C K F  + NL  H+ THTGE+PYKC+ C+  F  SSN++ H+R
Sbjct:  116 YKCKQCSKAFTTSGNLQSHILTHTGEKPYKCSICNWKFISSSNLRTHIR 164          

HSP 7 Score: 50.447 bits (119), Expect = 2.243e-7
Identity = 29/99 (29.29%), Postives = 49/99 (49.49%), Query Frame = 3
Query:  597 CRSQVSLIRHRKQHQNPNSH---YSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYTQYSNLCRHKRMSRDCQRKAKCTECGMEFTNGKTYDTH 884
            C    +L  H K H   ++    Y+C++C + F   + L++H+  H+  KP+ C  C K++   S+L RH R+    +R  KC +C   FT      +H
Sbjct:   37 CPKAFALAAHLKIHNRIHTGEKPYTCNICSRGFAQLTNLQRHILTHTGEKPHKCTYCPKAFVSSSDLRRHVRIHTG-ERPYKCKQCSKAFTTSGNLQSH 134          

HSP 8 Score: 49.6766 bits (117), Expect = 4.665e-7
Identity = 26/76 (34.21%), Postives = 39/76 (51.32%), Query Frame = 3
Query:  657 YSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYTQYSNLCRHKRMSRDCQRKAKCTECGMEFTNGKTYDTH 884
            Y C  C K F +++ LK H  IH+  KPY+C  C + + Q +NL RH  ++   ++  KCT C   F +      H
Sbjct:   32 YKCQKCPKAFALAAHLKIHNRIHTGEKPYTCNICSRGFAQLTNLQRHI-LTHTGEKPHKCTYCPKAFVSSSDLRRH 106          

HSP 9 Score: 47.7506 bits (112), Expect = 2.052e-6
Identity = 24/53 (45.28%), Postives = 30/53 (56.60%), Query Frame = 3
Query: 1590 EQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIR 1748
            ++PY C  C K F+ S N QRHV  IH  ++PYKC KC + F     L  H R
Sbjct:    1 DKPYTCQHCSKVFTRSWNFQRHVL-IHTGQKPYKCQKCPKAFALAAHLKIHNR 52          
BLAST of SMED30024013 vs. Ensembl Nematostella
Match: EDO46333 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RQB7])

HSP 1 Score: 84.7297 bits (208), Expect = 5.553e-18
Identity = 35/77 (45.45%), Postives = 55/77 (71.43%), Query Frame = 3
Query: 1521 CHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIRQ 1751
            CH+C K F ++++LN+H+R H+GE+PYKC +CDK F+ SS ++ H+R  H+ E+P+KC  C R F    + D H+R+
Sbjct:  219 CHVCKKRFSQSSSLNKHMRVHSGERPYKCTYCDKAFTASSILRTHIRQ-HSGEKPFKCKHCGRAFASHAAHDSHVRR 294          

HSP 2 Score: 63.5438 bits (153), Expect = 5.571e-11
Identity = 30/77 (38.96%), Postives = 41/77 (53.25%), Query Frame = 3
Query: 1515 YHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHI 1745
            Y C  C K F  ++ L  H+R H+GE+P+KC  C + F+  +    HVR  H KE+P  C  C + F Q   L  HI
Sbjct:  245 YKCTYCDKAFTASSILRTHIRQHSGEKPFKCKHCGRAFASHAAHDSHVRRTHTKEKPCICEYCGKAFAQSYELKFHI 321          

HSP 3 Score: 55.8398 bits (133), Expect = 1.622e-8
Identity = 33/103 (32.04%), Postives = 49/103 (47.57%), Query Frame = 3
Query:  576 CDVCNKPCRSQVSLIRHRKQHQNPNSHYSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYTQYSNLCRHKRMSRDCQRKAKCTECGMEFTNGKTYDTH 884
            C+ C++    +  L  H       +  + C VC K F  SS L +HM +HS  +PY C  C K++T  S L  H R     ++  KC  CG  F +   +D+H
Sbjct:  190 CEQCDRSFDKRDRLRIHVLHVHEKHRPHECHVCKKRFSQSSSLNKHMRVHSGERPYKCTYCDKAFTASSILRTHIRQHSG-EKPFKCKHCGRAFASHAAHDSH 291          

HSP 4 Score: 53.9138 bits (128), Expect = 8.025e-8
Identity = 27/82 (32.93%), Postives = 43/82 (52.44%), Query Frame = 3
Query: 1515 YHCHLCLKTFPRAANLNRHLR----THTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIR 1748
            Y C  C K F       RHL+       G++ + C  CD+ F     ++ HV ++H K RP++C  C + F Q +SL++H+R
Sbjct:  156 YSCERCGKVFAYKYYRERHLKYTRCVDQGDRRFPCEQCDRSFDKRDRLRIHVLHVHEKHRPHECHVCKKRFSQSSSLNKHMR 237          

HSP 5 Score: 53.1434 bits (126), Expect = 1.525e-7
Identity = 37/126 (29.37%), Postives = 60/126 (47.62%), Query Frame = 3
Query:  546 ISNGSNLNLFCDVCNKPCRSQVSLIRHRKQHQ---NPNSHYSCDVCGKTFKISSGLKQH-MHIHSSNKPYSCLNCQKSYTQYSNLCRHKRMSRDCQRKAKCTECGMEFTNGKTYDTHKQHCLGLDP 911
            IS G+  N  C+ C K    +    RH K  +     +  + C+ C ++F     L+ H +H+H  ++P+ C  C+K ++Q S+L +H R+    +R  KCT C   FT      TH +   G  P
Sbjct:  148 ISEGAPGNYSCERCGKVFAYKYYRERHLKYTRCVDQGDRRFPCEQCDRSFDKRDRLRIHVLHVHEKHRPHECHVCKKRFSQSSSLNKHMRVHSG-ERPYKCTYCDKAFTASSILRTHIRQHSGEKP 272          

HSP 6 Score: 50.447 bits (119), Expect = 1.069e-6
Identity = 29/96 (30.21%), Postives = 43/96 (44.79%), Query Frame = 3
Query:  576 CDVCNKPCRSQVSLIRHRKQHQNPNSHYSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYTQYSNLCRHKRMSRDCQRKAKCTECGMEFTN 863
            C VC K      SL +H + H      Y C  C K F  SS L+ H+  HS  KP+ C +C +++  ++    H R +   ++   C  CG  F  
Sbjct:  219 CHVCKKRFSQSSSLNKHMRVHSGERP-YKCTYCDKAFTASSILRTHIRQHSGEKPFKCKHCGRAFASHAAHDSHVRRTHTKEKPCICEYCGKAFAQ 313          
BLAST of SMED30024013 vs. Ensembl Medaka
Match: mecom (MDS1 and EVI1 complex locus [Source:NCBI gene;Acc:101169474])

HSP 1 Score: 131.339 bits (329), Expect = 1.965e-31
Identity = 55/80 (68.75%), Postives = 67/80 (83.75%), Query Frame = 3
Query: 1512 RYHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIRQ 1751
            RY C  C K FPR+ANL RHLRTHTGEQPY+C +CD+ FSISSN+QRH+RNIHNKE+P+KC  CDR F Q T+LDRH+++
Sbjct:  748 RYTCRYCGKIFPRSANLTRHLRTHTGEQPYRCKYCDRSFSISSNLQRHIRNIHNKEKPFKCHLCDRCFGQQTNLDRHLKK 827          

HSP 2 Score: 119.013 bits (297), Expect = 1.568e-27
Identity = 80/227 (35.24%), Postives = 113/227 (49.78%), Query Frame = 3
Query:  276 QFQCPDCGANFSSRGPLKRHQNALCKNP-----------NGLLIKTRQTIFKHNSV------IQRKQVLGTNQNCTTTNLSGGSEHRKTSSDYKQSVSFPISKLSSPISNGSNLNLFCDVCNKPCRSQVSLIRH-RKQHQNPNSHYSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYTQYSNLCRHKRMSRDCQRKAKCTECGMEFTNGKTYDTHKQHCLG 902
            Q++C DC  +F SR  L  HQ   C  P           NGL     + +  ++S        +  QV    Q+     LS   E         ++ ++  + +   +S+ S  +  C+ C+K      +L RH R QH    +H +C  CGKTF  SSGLKQH HIHSS KP+ C  C KSYTQ+SNLCRHKRM  DC+ + KC +CG  F+   + + H++ C G
Sbjct:   20 QYRCEDCDQHFESRNQLLDHQKQPCGGPPSSFLNSEEDFNGLKDHEPEDLRPYSSSHGLHECKECDQVFPDVQSLEAHMLSHSEEREYKCDQCPKAFNWKSNLIRHQMSHDSGKHYECENCSKVFTDPSNLQRHIRSQHVGARAH-ACSDCGKTFATSSGLKQHKHIHSSVKPFMCEVCHKSYTQFSNLCRHKRMHADCRTQIKCKDCGQMFSTTSSLNKHRRFCEG 245          

HSP 3 Score: 59.6918 bits (143), Expect = 8.301e-9
Identity = 28/78 (35.90%), Postives = 45/78 (57.69%), Query Frame = 3
Query: 1515 YHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIR 1748
            + C  C + FP   +L  H+ +H+ E+ YKC  C K F+  SN+ RH  + H+  + Y+C  C ++F   ++L RHIR
Sbjct:   79 HECKECDQVFPDVQSLEAHMLSHSEEREYKCDQCPKAFNWKSNLIRHQMS-HDSGKHYECENCSKVFTDPSNLQRHIR 155          

HSP 4 Score: 58.151 bits (139), Expect = 2.008e-8
Identity = 28/77 (36.36%), Postives = 41/77 (53.25%), Query Frame = 3
Query: 1512 RYHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRH 1742
             Y C  C K F   +NL RH  +H   + Y+C  C K F+  SN+QRH+R+ H   R + C+ C + F   + L +H
Sbjct:  106 EYKCDQCPKAFNWKSNLIRHQMSHDSGKHYECENCSKVFTDPSNLQRHIRSQHVGARAHACSDCGKTFATSSGLKQH 182          

HSP 5 Score: 56.225 bits (134), Expect = 8.471e-8
Identity = 25/56 (44.64%), Postives = 36/56 (64.29%), Query Frame = 3
Query: 1587 GEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIRQL 1754
            G++ Y C +C K F  S+N+ RH+R  H  E+PY+C  CDR F   ++L RHIR +
Sbjct:  745 GKERYTCRYCGKIFPRSANLTRHLR-THTGEQPYRCKYCDRSFSISSNLQRHIRNI 799          

HSP 6 Score: 53.1434 bits (126), Expect = 8.266e-7
Identity = 29/80 (36.25%), Postives = 42/80 (52.50%), Query Frame = 3
Query:  657 YSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYTQYSNLCRHKRMSRDCQRKAKCTECGMEFTNGKTYDTH-KQH 893
            Y+C  CGK F  S+ L +H+  H+  +PY C  C +S++  SNL RH R   + ++  KC  C   F      D H K+H
Sbjct:  749 YTCRYCGKIFPRSANLTRHLRTHTGEQPYRCKYCDRSFSISSNLQRHIRNIHNKEKPFKCHLCDRCFGQQTNLDRHLKKH 828          
BLAST of SMED30024013 vs. Ensembl Medaka
Match: mecom (MDS1 and EVI1 complex locus [Source:NCBI gene;Acc:101169474])

HSP 1 Score: 130.954 bits (328), Expect = 2.463e-31
Identity = 55/80 (68.75%), Postives = 67/80 (83.75%), Query Frame = 3
Query: 1512 RYHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIRQ 1751
            RY C  C K FPR+ANL RHLRTHTGEQPY+C +CD+ FSISSN+QRH+RNIHNKE+P+KC  CDR F Q T+LDRH+++
Sbjct:  910 RYTCRYCGKIFPRSANLTRHLRTHTGEQPYRCKYCDRSFSISSNLQRHIRNIHNKEKPFKCHLCDRCFGQQTNLDRHLKK 989          

HSP 2 Score: 116.701 bits (291), Expect = 1.016e-26
Identity = 79/227 (34.80%), Postives = 111/227 (48.90%), Query Frame = 3
Query:  276 QFQCPDCGANFSSRGPLKRHQNALCKNPNGLLIKTRQT--IFKHNSVIQR--------------KQVLGTNQNCTTTNLSGGSEHRKTSSDYKQSVSFPISKLSSPISNGSNLNLFCDVCNKPCRSQVS-LIRH-RKQHQNPNSHYSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYTQYSNLCRHKRMSRDCQRKAKCTECGMEFTNGKTYDTHKQHCLG 902
            Q++C DC  +F SR  L  HQ   C  P    + + +   +  H     R               QV    Q+     LS   E         ++ ++  + +   +S+ S  +  C+ C+K   +  S L RH R QH    +H +C  CGKTF  SSGLKQH HIHSS KP+ C  C KSYTQ+SNLCRHKRM  DC+ + KC +CG  F+   + + H++ C G
Sbjct:  209 QYRCEDCDQHFESRNQLLDHQKQPCGGPPSSFLNSGEDSGLKDHEPEDLRPYSSSHGLHECKECDQVFPDVQSLEAHMLSHSEEREYKCDQCPKAFNWKSNLIRHQMSHDSGKHYECENCSKQVFTDPSNLQRHIRSQHVGARAH-ACSDCGKTFATSSGLKQHKHIHSSVKPFMCEVCHKSYTQFSNLCRHKRMHADCRTQIKCKDCGQMFSTTSSLNKHRRFCEG 434          

HSP 3 Score: 56.225 bits (134), Expect = 9.635e-8
Identity = 25/56 (44.64%), Postives = 36/56 (64.29%), Query Frame = 3
Query: 1587 GEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIRQL 1754
            G++ Y C +C K F  S+N+ RH+R  H  E+PY+C  CDR F   ++L RHIR +
Sbjct:  907 GKERYTCRYCGKIFPRSANLTRHLR-THTGEQPYRCKYCDRSFSISSNLQRHIRNI 961          

HSP 4 Score: 55.0694 bits (131), Expect = 1.994e-7
Identity = 28/79 (35.44%), Postives = 45/79 (56.96%), Query Frame = 3
Query: 1515 YHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDR-IFDQLTSLDRHIR 1748
            + C  C + FP   +L  H+ +H+ E+ YKC  C K F+  SN+ RH  + H+  + Y+C  C + +F   ++L RHIR
Sbjct:  267 HECKECDQVFPDVQSLEAHMLSHSEEREYKCDQCPKAFNWKSNLIRHQMS-HDSGKHYECENCSKQVFTDPSNLQRHIR 344          

HSP 5 Score: 53.9138 bits (128), Expect = 4.652e-7
Identity = 28/78 (35.90%), Postives = 41/78 (52.56%), Query Frame = 3
Query: 1512 RYHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDK-CFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRH 1742
             Y C  C K F   +NL RH  +H   + Y+C  C K  F+  SN+QRH+R+ H   R + C+ C + F   + L +H
Sbjct:  294 EYKCDQCPKAFNWKSNLIRHQMSHDSGKHYECENCSKQVFTDPSNLQRHIRSQHVGARAHACSDCGKTFATSSGLKQH 371          

HSP 6 Score: 53.1434 bits (126), Expect = 7.074e-7
Identity = 29/80 (36.25%), Postives = 42/80 (52.50%), Query Frame = 3
Query:  657 YSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYTQYSNLCRHKRMSRDCQRKAKCTECGMEFTNGKTYDTH-KQH 893
            Y+C  CGK F  S+ L +H+  H+  +PY C  C +S++  SNL RH R   + ++  KC  C   F      D H K+H
Sbjct:  911 YTCRYCGKIFPRSANLTRHLRTHTGEQPYRCKYCDRSFSISSNLQRHIRNIHNKEKPFKCHLCDRCFGQQTNLDRHLKKH 990          
BLAST of SMED30024013 vs. Ensembl Medaka
Match: mecom (MDS1 and EVI1 complex locus [Source:NCBI gene;Acc:101169474])

HSP 1 Score: 130.954 bits (328), Expect = 2.528e-31
Identity = 55/80 (68.75%), Postives = 67/80 (83.75%), Query Frame = 3
Query: 1512 RYHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIRQ 1751
            RY C  C K FPR+ANL RHLRTHTGEQPY+C +CD+ FSISSN+QRH+RNIHNKE+P+KC  CDR F Q T+LDRH+++
Sbjct:  946 RYTCRYCGKIFPRSANLTRHLRTHTGEQPYRCKYCDRSFSISSNLQRHIRNIHNKEKPFKCHLCDRCFGQQTNLDRHLKK 1025          

HSP 2 Score: 110.153 bits (274), Expect = 1.137e-24
Identity = 79/235 (33.62%), Postives = 112/235 (47.66%), Query Frame = 3
Query:  276 QFQCPDCGANFSSRGPLKRHQNALCKNPNGLLIKTRQT--IFKHNSVIQR--------------KQVLGTNQNCTTTNLSGGSEHRKTSSDYKQSVSFPISKLSSPISNGSNLNLFCDVCNKPCRSQVS-LIRH-RKQHQNPNSHYSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLN--------CQKSYTQYSNLCRHKRMSRDCQRKAKCTECGMEFTNGKTYDTHKQHCLG 902
            Q++C DC  +F SR  L  HQ   C  P    + + +   +  H     R               QV    Q+     LS   E         ++ ++  + +   +S+ S  +  C+ C+K   +  S L RH R QH    +H +C  CGKTF  SSGLKQH HIHSS KP+ C +        C KSYTQ+SNLCRHKRM  DC+ + KC +CG  F+   + + H++ C G
Sbjct:  209 QYRCEDCDQHFESRNQLLDHQKQPCGGPPSSFLNSGEDSGLKDHEPEDLRPYSSSHGLHECKECDQVFPDVQSLEAHMLSHSEEREYKCDQCPKAFNWKSNLIRHQMSHDSGKHYECENCSKQVFTDPSNLQRHIRSQHVGARAH-ACSDCGKTFATSSGLKQHKHIHSSVKPFMCKSLRPYLCEVCHKSYTQFSNLCRHKRMHADCRTQIKCKDCGQMFSTTSSLNKHRRFCEG 442          

HSP 3 Score: 56.225 bits (134), Expect = 9.370e-8
Identity = 25/56 (44.64%), Postives = 36/56 (64.29%), Query Frame = 3
Query: 1587 GEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIRQL 1754
            G++ Y C +C K F  S+N+ RH+R  H  E+PY+C  CDR F   ++L RHIR +
Sbjct:  943 GKERYTCRYCGKIFPRSANLTRHLR-THTGEQPYRCKYCDRSFSISSNLQRHIRNI 997          

HSP 4 Score: 55.0694 bits (131), Expect = 1.842e-7
Identity = 28/79 (35.44%), Postives = 45/79 (56.96%), Query Frame = 3
Query: 1515 YHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDR-IFDQLTSLDRHIR 1748
            + C  C + FP   +L  H+ +H+ E+ YKC  C K F+  SN+ RH  + H+  + Y+C  C + +F   ++L RHIR
Sbjct:  267 HECKECDQVFPDVQSLEAHMLSHSEEREYKCDQCPKAFNWKSNLIRHQMS-HDSGKHYECENCSKQVFTDPSNLQRHIR 344          

HSP 5 Score: 54.299 bits (129), Expect = 3.877e-7
Identity = 28/78 (35.90%), Postives = 41/78 (52.56%), Query Frame = 3
Query: 1512 RYHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDK-CFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRH 1742
             Y C  C K F   +NL RH  +H   + Y+C  C K  F+  SN+QRH+R+ H   R + C+ C + F   + L +H
Sbjct:  294 EYKCDQCPKAFNWKSNLIRHQMSHDSGKHYECENCSKQVFTDPSNLQRHIRSQHVGARAHACSDCGKTFATSSGLKQH 371          

HSP 6 Score: 53.1434 bits (126), Expect = 7.817e-7
Identity = 29/80 (36.25%), Postives = 42/80 (52.50%), Query Frame = 3
Query:  657 YSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYTQYSNLCRHKRMSRDCQRKAKCTECGMEFTNGKTYDTH-KQH 893
            Y+C  CGK F  S+ L +H+  H+  +PY C  C +S++  SNL RH R   + ++  KC  C   F      D H K+H
Sbjct:  947 YTCRYCGKIFPRSANLTRHLRTHTGEQPYRCKYCDRSFSISSNLQRHIRNIHNKEKPFKCHLCDRCFGQQTNLDRHLKKH 1026          
BLAST of SMED30024013 vs. Ensembl Medaka
Match: mecom (MDS1 and EVI1 complex locus [Source:NCBI gene;Acc:101169474])

HSP 1 Score: 130.954 bits (328), Expect = 2.694e-31
Identity = 55/80 (68.75%), Postives = 67/80 (83.75%), Query Frame = 3
Query: 1512 RYHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIRQ 1751
            RY C  C K FPR+ANL RHLRTHTGEQPY+C +CD+ FSISSN+QRH+RNIHNKE+P+KC  CDR F Q T+LDRH+++
Sbjct:  946 RYTCRYCGKIFPRSANLTRHLRTHTGEQPYRCKYCDRSFSISSNLQRHIRNIHNKEKPFKCHLCDRCFGQQTNLDRHLKK 1025          

HSP 2 Score: 110.153 bits (274), Expect = 1.237e-24
Identity = 79/235 (33.62%), Postives = 112/235 (47.66%), Query Frame = 3
Query:  276 QFQCPDCGANFSSRGPLKRHQNALCKNPNGLLIKTRQT--IFKHNSVIQR--------------KQVLGTNQNCTTTNLSGGSEHRKTSSDYKQSVSFPISKLSSPISNGSNLNLFCDVCNKPCRSQVS-LIRH-RKQHQNPNSHYSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLN--------CQKSYTQYSNLCRHKRMSRDCQRKAKCTECGMEFTNGKTYDTHKQHCLG 902
            Q++C DC  +F SR  L  HQ   C  P    + + +   +  H     R               QV    Q+     LS   E         ++ ++  + +   +S+ S  +  C+ C+K   +  S L RH R QH    +H +C  CGKTF  SSGLKQH HIHSS KP+ C +        C KSYTQ+SNLCRHKRM  DC+ + KC +CG  F+   + + H++ C G
Sbjct:  209 QYRCEDCDQHFESRNQLLDHQKQPCGGPPSSFLNSGEDSGLKDHEPEDLRPYSSSHGLHECKECDQVFPDVQSLEAHMLSHSEEREYKCDQCPKAFNWKSNLIRHQMSHDSGKHYECENCSKQVFTDPSNLQRHIRSQHVGARAH-ACSDCGKTFATSSGLKQHKHIHSSVKPFMCKSLRPYLCEVCHKSYTQFSNLCRHKRMHADCRTQIKCKDCGQMFSTTSSLNKHRRFCEG 442          

HSP 3 Score: 56.225 bits (134), Expect = 9.251e-8
Identity = 25/56 (44.64%), Postives = 36/56 (64.29%), Query Frame = 3
Query: 1587 GEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIRQL 1754
            G++ Y C +C K F  S+N+ RH+R  H  E+PY+C  CDR F   ++L RHIR +
Sbjct:  943 GKERYTCRYCGKIFPRSANLTRHLR-THTGEQPYRCKYCDRSFSISSNLQRHIRNI 997          

HSP 4 Score: 55.0694 bits (131), Expect = 1.999e-7
Identity = 28/79 (35.44%), Postives = 45/79 (56.96%), Query Frame = 3
Query: 1515 YHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDR-IFDQLTSLDRHIR 1748
            + C  C + FP   +L  H+ +H+ E+ YKC  C K F+  SN+ RH  + H+  + Y+C  C + +F   ++L RHIR
Sbjct:  267 HECKECDQVFPDVQSLEAHMLSHSEEREYKCDQCPKAFNWKSNLIRHQMS-HDSGKHYECENCSKQVFTDPSNLQRHIR 344          

HSP 5 Score: 54.299 bits (129), Expect = 3.964e-7
Identity = 28/78 (35.90%), Postives = 41/78 (52.56%), Query Frame = 3
Query: 1512 RYHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDK-CFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRH 1742
             Y C  C K F   +NL RH  +H   + Y+C  C K  F+  SN+QRH+R+ H   R + C+ C + F   + L +H
Sbjct:  294 EYKCDQCPKAFNWKSNLIRHQMSHDSGKHYECENCSKQVFTDPSNLQRHIRSQHVGARAHACSDCGKTFATSSGLKQH 371          

HSP 6 Score: 53.1434 bits (126), Expect = 7.277e-7
Identity = 29/80 (36.25%), Postives = 42/80 (52.50%), Query Frame = 3
Query:  657 YSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYTQYSNLCRHKRMSRDCQRKAKCTECGMEFTNGKTYDTH-KQH 893
            Y+C  CGK F  S+ L +H+  H+  +PY C  C +S++  SNL RH R   + ++  KC  C   F      D H K+H
Sbjct:  947 YTCRYCGKIFPRSANLTRHLRTHTGEQPYRCKYCDRSFSISSNLQRHIRNIHNKEKPFKCHLCDRCFGQQTNLDRHLKKH 1026          
BLAST of SMED30024013 vs. Ensembl Medaka
Match: si:ch211-263k4.2 (PR/SET domain 16 [Source:NCBI gene;Acc:101156011])

HSP 1 Score: 130.183 bits (326), Expect = 5.271e-31
Identity = 55/80 (68.75%), Postives = 67/80 (83.75%), Query Frame = 3
Query: 1512 RYHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIRQ 1751
            RY C  C K FPR+ANL RHLRTHTGEQPY+C +CD+ FSISSN+QRHVRNIHNKE+P+KC  C+R F Q T+LDRH+++
Sbjct: 1057 RYACRYCGKIFPRSANLTRHLRTHTGEQPYRCKYCDRSFSISSNLQRHVRNIHNKEKPFKCHLCNRCFGQQTNLDRHLKK 1136          

HSP 2 Score: 114.005 bits (284), Expect = 7.796e-26
Identity = 79/229 (34.50%), Postives = 115/229 (50.22%), Query Frame = 3
Query:  264 QASSQFQCPDCGANFSSRGPLKRHQNALCKNPNGLLIKTRQTIFKHNSVIQRKQV--------LGTNQNCTTTNLSGGSEHRKTSSDY-KQSVSFPISKLSSPISNGSNLNLFCDVCNKPCRSQ------VSLIRH-RKQHQNPNSHYSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYTQYSNLCRHKRMSRDCQRKAKCTECGMEFTNGKTYDTHKQHCLG 902
            Q    ++C DC   FSS   L+RHQ   C +   +    R   FK       + V        +  N+     ++   +E R+   D   ++ ++  + +   +S+ S     C+ C+K   +Q       +L RH R QH    +H +C  CGKTF  SSGLKQH HIHSS KP+ C  C KSYTQ+SNLCRHKRM  DC+ + KC +CG  F+   + + H++ C G
Sbjct:  253 QDEQMYRCEDCDELFSSTLELRRHQKYSCSSAGSIFDALRDD-FKQEREDSDEPVHECKDCEKIFPNEYSLGQHMIVHTEEREYKCDQCPKAFNWKSNLIRHQMSHDSGKRFECENCDKVQHTQHVFTDPSNLQRHIRSQHVGARAH-TCPECGKTFATSSGLKQHKHIHSSVKPFICEVCHKSYTQFSNLCRHKRMHADCRTQIKCKDCGQLFSTTSSLNKHRRFCEG 479          

HSP 3 Score: 57.3806 bits (137), Expect = 4.558e-8
Identity = 30/84 (35.71%), Postives = 46/84 (54.76%), Query Frame = 3
Query: 1515 YHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDR------IFDQLTSLDRHIR 1748
            + C  C K FP   +L +H+  HT E+ YKC  C K F+  SN+ RH  + H+  + ++C  CD+      +F   ++L RHIR
Sbjct:  307 HECKDCEKIFPNEYSLGQHMIVHTEEREYKCDQCPKAFNWKSNLIRHQMS-HDSGKRFECENCDKVQHTQHVFTDPSNLQRHIR 389          

HSP 4 Score: 56.6102 bits (135), Expect = 7.234e-8
Identity = 24/56 (42.86%), Postives = 36/56 (64.29%), Query Frame = 3
Query: 1587 GEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIRQL 1754
            G++ Y C +C K F  S+N+ RH+R  H  E+PY+C  CDR F   ++L RH+R +
Sbjct: 1054 GKERYACRYCGKIFPRSANLTRHLR-THTGEQPYRCKYCDRSFSISSNLQRHVRNI 1108          

HSP 5 Score: 54.299 bits (129), Expect = 3.766e-7
Identity = 29/80 (36.25%), Postives = 42/80 (52.50%), Query Frame = 3
Query:  657 YSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYTQYSNLCRHKRMSRDCQRKAKCTECGMEFTNGKTYDTH-KQH 893
            Y+C  CGK F  S+ L +H+  H+  +PY C  C +S++  SNL RH R   + ++  KC  C   F      D H K+H
Sbjct: 1058 YACRYCGKIFPRSANLTRHLRTHTGEQPYRCKYCDRSFSISSNLQRHVRNIHNKEKPFKCHLCNRCFGQQTNLDRHLKKH 1137          

HSP 6 Score: 53.9138 bits (128), Expect = 5.301e-7
Identity = 28/83 (33.73%), Postives = 42/83 (50.60%), Query Frame = 3
Query: 1512 RYHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKC------FSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRH 1742
             Y C  C K F   +NL RH  +H   + ++C  CDK       F+  SN+QRH+R+ H   R + C +C + F   + L +H
Sbjct:  334 EYKCDQCPKAFNWKSNLIRHQMSHDSGKRFECENCDKVQHTQHVFTDPSNLQRHIRSQHVGARAHTCPECGKTFATSSGLKQH 416          
BLAST of SMED30024013 vs. Planmine SMEST
Match: SMESG000059642.1 (SMESG000059642.1)

HSP 1 Score: 1067.37 bits (2759), Expect = 0.000e+0
Identity = 579/580 (99.83%), Postives = 580/580 (100.00%), Query Frame = 3
Query:   24 MNFAKLTGESQSGFLFTSGLMNGEKSQIPLPFFDSNWKDVNTNEWCSLKLDHLGLNYQEKLENLPTFIASVANIYSKSTTQASSQFQCPDCGANFSSRGPLKRHQNALCKNPNGLLIKTRQTIFKHNSVIQRKQVLGTNQNCTTTNLSGGSEHRKTSSDYKQSVSFPISKLSSPISNGSNLNLFCDVCNKPCRSQVSLIRHRKQHQNPNSHYSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYTQYSNLCRHKRMSRDCQRKAKCTECGMEFTNGKTYDTHKQHCLGLDPSGYLKSPXXXXXXXXXXXXXXXXXXXXXXTWSSPNVHIQQSATVKSFNTEDLIREIVPLDLTVRKSKTDCPTSQTFPDSRVEQLIKWNNLNKCGGNNSIQSLNPNHLNSINSMASAFPSNCHGNDYMSSINQYFLQFPVMIPRPRXXXXXXXXXXXXXXXXKHHNADDKKSVNTVIELAXXXXXXXXXXXXXXXXXXXTIRYHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIRQLCSM 1763
            MNFAKLTGESQSGFLFTSGLMNGEKSQIPLPFFDSNWKDVNTNEWCSLKLDHLGLNYQEKLENLPTFIASVANIYSKSTTQASSQFQCPDCGANFSSRGPLKRHQNALCKNPNGLLIKTRQTIFKHNSVIQRKQVLGTNQNCTTTNLSGGSEHRKTSSDYKQSVSFPISKLSSPISNGSNLNLFCDVCNKPCRSQVSLIRHRKQHQNPNSHYSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYTQYSNLCRHKRMSRDCQRKAKCTECGMEFTNGKTYDTHKQHCLGLDPSGYLKSPSYSSQANSINNNNNNSGNIIESTWSSPNVHIQQSATVKSFNTEDLIREIVPLDLTVRKSKTDCPTS+TFPDSRVEQLIKWNNLNKCGGNNSIQSLNPNHLNSINSMASAFPSNCHGNDYMSSINQYFLQFPVMIPRPRSLNLDNSKSSSSINSLKHHNADDKKSVNTVIELASSKSHTQSSRCSSSFSSSSTIRYHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIRQLCSM
Sbjct:    1 MNFAKLTGESQSGFLFTSGLMNGEKSQIPLPFFDSNWKDVNTNEWCSLKLDHLGLNYQEKLENLPTFIASVANIYSKSTTQASSQFQCPDCGANFSSRGPLKRHQNALCKNPNGLLIKTRQTIFKHNSVIQRKQVLGTNQNCTTTNLSGGSEHRKTSSDYKQSVSFPISKLSSPISNGSNLNLFCDVCNKPCRSQVSLIRHRKQHQNPNSHYSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYTQYSNLCRHKRMSRDCQRKAKCTECGMEFTNGKTYDTHKQHCLGLDPSGYLKSPSYSSQANSINNNNNNSGNIIESTWSSPNVHIQQSATVKSFNTEDLIREIVPLDLTVRKSKTDCPTSETFPDSRVEQLIKWNNLNKCGGNNSIQSLNPNHLNSINSMASAFPSNCHGNDYMSSINQYFLQFPVMIPRPRSLNLDNSKSSSSINSLKHHNADDKKSVNTVIELASSKSHTQSSRCSSSFSSSSTIRYHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIRQLCSM 580          
BLAST of SMED30024013 vs. Planmine SMEST
Match: SMESG000028951.1 (SMESG000028951.1)

HSP 1 Score: 123.25 bits (308), Expect = 1.110e-30
Identity = 52/79 (65.82%), Postives = 64/79 (81.01%), Query Frame = 3
Query: 1512 RYHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIR 1748
            RYHC  C K FPR+ANL RH+RTHTGEQPYKC+FC +CFSISSN+QRH+RNIH KERP+ C  C + F Q  +L+RH++
Sbjct:  243 RYHCRFCGKLFPRSANLTRHVRTHTGEQPYKCSFCTRCFSISSNLQRHIRNIHQKERPFNCDICFKRFGQRANLERHVK 321          

HSP 2 Score: 64.6994 bits (156), Expect = 4.761e-11
Identity = 28/49 (57.14%), Postives = 35/49 (71.43%), Query Frame = 3
Query:  657 YSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYTQYSNLCRHKR 803
            ++C  C K F   +GLKQH H HS+ KPY C  C K+YTQ+SNLCRHK+
Sbjct:   13 FTCTDCDKIFNSEAGLKQHRHTHSTFKPYRCTICPKAYTQFSNLCRHKK 61          

HSP 3 Score: 53.9138 bits (128), Expect = 1.736e-7
Identity = 26/52 (50.00%), Postives = 33/52 (63.46%), Query Frame = 3
Query: 1599 YKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIRQL 1754
            Y C FC K F  S+N+ RHVR  H  E+PYKC+ C R F   ++L RHIR +
Sbjct:  244 YHCRFCGKLFPRSANLTRHVR-THTGEQPYKCSFCTRCFSISSNLQRHIRNI 294          

HSP 4 Score: 50.8322 bits (120), Expect = 1.437e-6
Identity = 47/156 (30.13%), Postives = 67/156 (42.95%), Query Frame = 3
Query:  483 RKTSSDYKQSVSFPISK-LSSPISNGSNLNLFCDVCNKPCRSQVSLIRHRKQHQNPNS---------HYSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYTQYSNLCRHKRMSRDCQRKAKCTECGMEFTNGKTYDTH-KQHCLGLDPSG 917
            R+TSS +    + PIS+ L+S   +   LN     C +P +S        K  + PNS          Y C  CGK F  S+ L +H+  H+  +PY C  C + ++  SNL RH R     +R   C  C   F      + H K H +    SG
Sbjct:  188 RQTSSYHNSIHNLPISRELNSSPPHIDQLN-----CEQPPKSY-------KSSETPNSPDSFIVKRDRYHCRFCGKLFPRSANLTRHVRTHTGEQPYKCSFCTRCFSISSNLQRHIRNIHQKERPFNCDICFKRFGQRANLERHVKNHLVNQQNSG 331          
BLAST of SMED30024013 vs. Planmine SMEST
Match: SMESG000063468.1 (SMESG000063468.1)

HSP 1 Score: 93.5893 bits (231), Expect = 1.836e-20
Identity = 41/51 (80.39%), Postives = 45/51 (88.24%), Query Frame = 3
Query: 1521 CHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHN 1673
            C +C K FPRAANLNRHLRTHTGEQPY C +C++ FSISSNMQRHVRNIHN
Sbjct:  296 CSVCGKIFPRAANLNRHLRTHTGEQPYHCPYCERSFSISSNMQRHVRNIHN 346          

HSP 2 Score: 83.5741 bits (205), Expect = 3.921e-17
Identity = 47/97 (48.45%), Postives = 59/97 (60.82%), Query Frame = 3
Query:  642 NPNSHYSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYTQYSNLCRHKRMSRDCQRKAKCTECGM--EFTNGKTYDTHKQHCLGLDPSGYLK 926
            NPN+   C +C K F   SGLKQHMHIH+S KP+ C  C K+YTQ+SNLCRHKRM + C+RK+      +     N  TY ++ Q     DP G LK
Sbjct:   10 NPNT---CPLCSKCFTSISGLKQHMHIHASFKPFRCQVCHKAYTQFSNLCRHKRMHKRCKRKSCFDSSNLIEPLINNNTYFSNLQ-----DPQGKLK 98          
BLAST of SMED30024013 vs. Planmine SMEST
Match: SMESG000000960.1 (SMESG000000960.1)

HSP 1 Score: 80.1073 bits (196), Expect = 3.971e-17
Identity = 39/80 (48.75%), Postives = 51/80 (63.75%), Query Frame = 3
Query: 1512 RYHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIRQ 1751
            ++ C+ C K+F R   L RH R HTGE+P  C FCDK F  S ++  HVR IH  E+PY+C+ CDR+F +     RHIRQ
Sbjct:  105 QFTCNTCKKSFYRRDELKRHERIHTGERPLACHFCDKRFMRSDHLSTHVR-IHTGEKPYQCSYCDRLFSRSDERLRHIRQ 183          

HSP 2 Score: 62.3882 bits (150), Expect = 4.875e-11
Identity = 25/50 (50.00%), Postives = 35/50 (70.00%), Query Frame = 3
Query: 1521 CHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIH 1670
            CH C K F R+ +L+ H+R HTGE+PY+C++CD+ FS S    RH+R  H
Sbjct:  136 CHFCDKRFMRSDHLSTHVRIHTGEKPYQCSYCDRLFSRSDERLRHIRQTH 185          

HSP 3 Score: 47.7506 bits (112), Expect = 4.465e-6
Identity = 30/98 (30.61%), Postives = 44/98 (44.90%), Query Frame = 3
Query:  534 LSSPISNGSNLNLF------CDVCNKPCRSQVSLIRHRKQHQNPNSHYSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYTQYSNLCRHKRMS 809
            +SSP+S  S  + F      C+ C K    +  L RH + H       +C  C K F  S  L  H+ IH+  KPY C  C + +++     RH R +
Sbjct:   88 ISSPLSEISEKSQFRIKQFTCNTCKKSFYRRDELKRHERIHTGERP-LACHFCDKRFMRSDHLSTHVRIHTGEKPYQCSYCDRLFSRSDERLRHIRQT 184          
BLAST of SMED30024013 vs. Planmine SMEST
Match: SMESG000000679.1 (SMESG000000679.1)

HSP 1 Score: 82.0333 bits (201), Expect = 2.416e-16
Identity = 35/78 (44.87%), Postives = 55/78 (70.51%), Query Frame = 3
Query: 1515 YHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIR 1748
            + CH+C +TF +++++  H+RTH+GE+PY+C  C K FS SS + +H R +H+ E+PYKC  C   F Q  +L+RH+R
Sbjct:  373 FSCHICCRTFSQSSSVTTHMRTHSGERPYRCHHCSKAFSDSSTLTKHTR-VHSGEKPYKCRICLLKFSQSGNLNRHMR 449          

HSP 2 Score: 76.2554 bits (186), Expect = 2.052e-14
Identity = 34/76 (44.74%), Postives = 50/76 (65.79%), Query Frame = 3
Query: 1521 CHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIR 1748
            CHLC +++ R + L  H+RTH+GE+P+KC  C K FS  +N+  H R IH+ E+P+ C  C R F Q +S+  H+R
Sbjct:  319 CHLCGRSYARPSTLKIHMRTHSGERPFKCNVCSKTFSQVANLTAHKR-IHSGEKPFSCHICCRTFSQSSSVTTHMR 393          

HSP 3 Score: 73.559 bits (179), Expect = 1.585e-13
Identity = 33/78 (42.31%), Postives = 50/78 (64.10%), Query Frame = 3
Query: 1515 YHCHLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHNKERPYKCTKCDRIFDQLTSLDRHIR 1748
            + C++C KTF + ANL  H R H+GE+P+ C  C + FS SS++  H+R  H+ ERPY+C  C + F   ++L +H R
Sbjct:  345 FKCNVCSKTFSQVANLTAHKRIHSGEKPFSCHICCRTFSQSSSVTTHMR-THSGERPYRCHHCSKAFSDSSTLTKHTR 421          

HSP 4 Score: 60.8474 bits (146), Expect = 1.382e-9
Identity = 32/103 (31.07%), Postives = 54/103 (52.43%), Query Frame = 3
Query:  576 CDVCNKPCRSQVSLIRHRKQHQNPNSHYSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYTQYSNLCRHKRMSRDCQRKAKCTECGMEFTNGKTYDTH 884
            C+VC+K      +L  H++ H      +SC +C +TF  SS +  HM  HS  +PY C +C K+++  S L +H R+    ++  KC  C ++F+     + H
Sbjct:  347 CNVCSKTFSQVANLTAHKRIHSGEKP-FSCHICCRTFSQSSSVTTHMRTHSGERPYRCHHCSKAFSDSSTLTKHTRV-HSGEKPYKCRICLLKFSQSGNLNRH 447          

HSP 5 Score: 55.0694 bits (131), Expect = 9.977e-8
Identity = 33/112 (29.46%), Postives = 53/112 (47.32%), Query Frame = 3
Query:  576 CDVCNKPCRSQVSLIRHRKQHQNPNSHYSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYTQYSNLCRHKRMSRDCQRKAKCTECGMEFTNGKTYDTHKQHCLGLDP 911
            C +C +      +L  H + H      + C+VC KTF   + L  H  IHS  KP+SC  C ++++Q S++  H R +   +R  +C  C   F++  T   H +   G  P
Sbjct:  319 CHLCGRSYARPSTLKIHMRTHSGERP-FKCNVCSKTFSQVANLTAHKRIHSGEKPFSCHICCRTFSQSSSVTTHMR-THSGERPYRCHHCSKAFSDSSTLTKHTRVHSGEKP 428          
The following BLAST results are available for this feature:
BLAST of SMED30024013 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
MECOM2.003e-3170.00MDS1 and EVI1 complex locus [Source:HGNC Symbol;Ac... [more]
MECOM2.224e-3170.00MDS1 and EVI1 complex locus [Source:HGNC Symbol;Ac... [more]
MECOM2.246e-3170.00MDS1 and EVI1 complex locus [Source:HGNC Symbol;Ac... [more]
MECOM2.246e-3170.00MDS1 and EVI1 complex locus [Source:HGNC Symbol;Ac... [more]
MECOM2.257e-3170.00MDS1 and EVI1 complex locus [Source:HGNC Symbol;Ac... [more]
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BLAST of SMED30024013 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
egl-431.374e-2276.36pep chromosome:WBcel235:II:9960333:9962344:1 gene:... [more]
egl-431.855e-2176.36pep chromosome:WBcel235:II:9955185:9962344:1 gene:... [more]
F47E1.32.239e-1645.45pep chromosome:WBcel235:X:9046213:9054885:1 gene:W... [more]
che-11.707e-1543.59Transcription factor che-1 [Source:UniProtKB/Swis... [more]
che-12.372e-1543.59Transcription factor che-1 [Source:UniProtKB/Swis... [more]
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BLAST of SMED30024013 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ham5.633e-3370.00gene:FBgn0045852 transcript:FBtr0332016[more]
CG103482.945e-3270.00gene:FBgn0032707 transcript:FBtr0343112[more]
CG103482.945e-3270.00gene:FBgn0032707 transcript:FBtr0301647[more]
ham7.292e-3270.00gene:FBgn0045852 transcript:FBtr0305621[more]
ham7.292e-3270.00gene:FBgn0045852 transcript:FBtr0332015[more]
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BLAST of SMED30024013 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
si:ch211-263k4.26.116e-3168.75si:ch211-263k4.2 [Source:ZFIN;Acc:ZDB-GENE-081104-... [more]
si:ch211-263k4.21.530e-3068.75si:ch211-263k4.2 [Source:ZFIN;Acc:ZDB-GENE-081104-... [more]
mecom4.274e-2345.74MDS1 and EVI1 complex locus [Source:ZFIN;Acc:ZDB-G... [more]
znf10801.700e-1850.00zinc finger protein 1080 [Source:ZFIN;Acc:ZDB-GENE... [more]
BX005085.14.340e-1846.15pep chromosome:GRCz11:3:7123912:7129057:-1 gene:EN... [more]
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BLAST of SMED30024013 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSXETT00000031589.13.606e-3270.00MDS1 and EVI1 complex locus protein EVI1-A-like [S... [more]
PRDM162.693e-3068.75PR domain 16 [Source:NCBI gene;Acc:100485690][more]
PRDM163.299e-3068.75PR domain 16 [Source:NCBI gene;Acc:100485690][more]
PRDM163.621e-3068.75PR domain 16 [Source:NCBI gene;Acc:100485690][more]
cip2a1.707e-1753.16cell proliferation regulating inhibitor of protein... [more]
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BLAST of SMED30024013 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Mecom1.540e-3170.00MDS1 and EVI1 complex locus [Source:MGI Symbol;Acc... [more]
Mecom1.564e-3170.00MDS1 and EVI1 complex locus [Source:MGI Symbol;Acc... [more]
Mecom1.602e-3170.00MDS1 and EVI1 complex locus [Source:MGI Symbol;Acc... [more]
Mecom1.717e-3170.00MDS1 and EVI1 complex locus [Source:MGI Symbol;Acc... [more]
Mecom2.066e-3170.00MDS1 and EVI1 complex locus [Source:MGI Symbol;Acc... [more]
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BLAST of SMED30024013 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q8I7Z8|HAM_DROME7.310e-3170.00Transcription factor hamlet OS=Drosophila melanoga... [more]
sp|Q03112|MECOM_HUMAN1.084e-3070.00Histone-lysine N-methyltransferase MECOM OS=Homo s... [more]
sp|P14404|MECOM_MOUSE1.322e-3070.00Histone-lysine N-methyltransferase MECOM OS=Mus mu... [more]
sp|B7ZRU9|EVI1A_XENLA1.620e-3070.00MDS1 and EVI1 complex locus protein EVI1-A OS=Xeno... [more]
sp|B7ZRM8|EVI1B_XENLA2.668e-3068.75MDS1 and EVI1 complex locus protein EVI1-B OS=Xeno... [more]
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BLAST of SMED30024013 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A0B1S9I43.613e-3270.00Zinc finger, C2H2 type OS=Oesophagostomum dentatum... [more]
A0A0C2D5P84.690e-3270.00Zinc finger, C2H2 type (Fragment) OS=Ancylostoma d... [more]
A0A1L8DNR81.117e-3170.00Putative conserved secreted protein OS=Nyssomyia n... [more]
A0A0D6LYC11.729e-3170.00Zinc finger, C2H2 type OS=Ancylostoma ceylanicum O... [more]
Q4RE091.853e-3170.00Chromosome undetermined SCAF15202, whole genome sh... [more]
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BLAST of SMED30024013 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
mecom2.827e-3168.75MDS1 and EVI1 complex locus [Source:ZFIN;Acc:ZDB-G... [more]
mecom3.471e-3168.75MDS1 and EVI1 complex locus [Source:ZFIN;Acc:ZDB-G... [more]
si:ch211-263k4.26.654e-3168.75PR/SET domain 16 [Source:NCBI gene;Acc:103028334][more]
si:ch211-263k4.27.193e-3168.75PR/SET domain 16 [Source:NCBI gene;Acc:103028334][more]
si:ch211-263k4.27.689e-3168.75PR/SET domain 16 [Source:NCBI gene;Acc:103028334][more]
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BLAST of SMED30024013 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSPMAT00000007831.16.470e-1946.05pep scaffold:Pmarinus_7.0:GL477655:47469:47963:1 g... [more]
ENSPMAT00000000449.11.296e-1746.15pep scaffold:Pmarinus_7.0:GL478964:311851:312750:-... [more]
ZNF4363.169e-1748.05zinc finger protein 436 [Source:HGNC Symbol;Acc:HG... [more]
ENSPMAT00000007400.15.348e-1762.00pep scaffold:Pmarinus_7.0:GL476367:705224:706108:-... [more]
ENSPMAT00000000923.19.003e-1646.15pep scaffold:Pmarinus_7.0:GL480775:18221:19434:-1 ... [more]
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BLAST of SMED30024013 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 5
Match NameE-valueIdentityDescription
ZAP12.418e-1341.03Zinc-regulated transcription factor; binds to zinc... [more]
AZF13.279e-1134.62Zinc-finger transcription factor; involved in diau... [more]
MSN27.668e-1046.00Stress-responsive transcriptional activator; activ... [more]
CRZ13.618e-933.33Transcription factor, activates transcription of s... [more]
ADR17.862e-940.38Carbon source-responsive zinc-finger transcription... [more]
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BLAST of SMED30024013 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO476923.578e-1947.44Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO328911.144e-1846.75Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO452591.149e-1848.72Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO491164.248e-1849.35Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO463335.553e-1845.45Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of SMED30024013 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
mecom1.965e-3168.75MDS1 and EVI1 complex locus [Source:NCBI gene;Acc:... [more]
mecom2.463e-3168.75MDS1 and EVI1 complex locus [Source:NCBI gene;Acc:... [more]
mecom2.528e-3168.75MDS1 and EVI1 complex locus [Source:NCBI gene;Acc:... [more]
mecom2.694e-3168.75MDS1 and EVI1 complex locus [Source:NCBI gene;Acc:... [more]
si:ch211-263k4.25.271e-3168.75PR/SET domain 16 [Source:NCBI gene;Acc:101156011][more]
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BLAST of SMED30024013 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000059642.10.000e+099.83SMESG000059642.1[more]
SMESG000028951.11.110e-3065.82SMESG000028951.1[more]
SMESG000063468.11.836e-2080.39SMESG000063468.1[more]
SMESG000000960.13.971e-1748.75SMESG000000960.1[more]
SMESG000000679.12.416e-1644.87SMESG000000679.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30024013 ID=SMED30024013|Name=SMED30024013|organism=Schmidtea mediterranea sexual|type=transcript|length=1790bp
TGTCACTCAATTAAAACAAAGAAATGAATTTCGCCAAACTCACCGGTGAG
AGTCAATCGGGATTTTTATTCACTTCCGGATTGATGAATGGGGAAAAATC
CCAAATCCCTCTGCCATTTTTCGACTCGAATTGGAAAGATGTCAATACAA
ATGAATGGTGCTCTTTGAAACTGGATCATTTAGGGTTGAATTATCAGGAG
AAACTTGAGAACTTACCAACATTTATTGCGTCTGTTGCCAATATCTATAG
CAAATCAACGACGCAGGCTTCTTCACAATTCCAGTGCCCTGACTGTGGCG
CAAACTTTTCATCGCGAGGCCCATTAAAACGACATCAAAATGCATTGTGT
AAAAATCCGAATGGACTTTTAATAAAAACTCGGCAAACCATTTTCAAGCA
TAACAGCGTTATACAGAGAAAGCAAGTATTGGGAACGAATCAGAATTGCA
CTACGACTAACTTATCCGGAGGATCAGAGCATCGTAAAACGAGTTCTGAT
TACAAGCAGTCGGTTTCATTTCCAATATCAAAATTATCAAGTCCGATCTC
AAACGGGTCGAACCTCAATCTGTTTTGCGACGTTTGCAATAAACCTTGTC
GAAGTCAAGTCAGTTTGATCAGGCATAGGAAGCAGCACCAGAATCCGAAT
TCCCATTATTCTTGTGATGTTTGTGGGAAAACGTTTAAAATAAGTTCCGG
ACTCAAACAGCACATGCACATTCACTCGTCCAATAAACCTTATTCTTGCT
TAAATTGCCAAAAGTCCTATACTCAATATAGTAATCTCTGTCGACATAAA
AGAATGAGTAGAGATTGTCAAAGAAAAGCAAAGTGTACTGAATGTGGAAT
GGAATTCACCAATGGTAAAACCTATGACACTCATAAACAGCATTGTTTAG
GTTTGGATCCAAGTGGATATTTGAAATCCCCATCTTACAGTTCTCAAGCA
AATTCCATAAATAATAATAATAATAATTCTGGCAATATTATTGAATCAAC
TTGGAGCAGTCCAAATGTACATATTCAACAGTCAGCCACTGTCAAGTCAT
TCAATACCGAGGACTTAATCAGAGAAATTGTCCCTTTGGATTTAACTGTT
AGAAAATCAAAAACTGACTGTCCAACTTCTCAAACCTTTCCCGATTCAAG
AGTTGAACAGTTGATCAAATGGAACAATTTAAATAAATGTGGGGGTAACA
ACAGCATTCAGTCTCTAAATCCCAATCACTTGAATTCCATAAATAGTATG
GCATCGGCATTTCCATCGAACTGCCATGGAAATGATTACATGAGTTCAAT
CAATCAATACTTTCTACAATTTCCAGTAATGATTCCTCGTCCCAGATCTT
TGAATCTCGATAATTCAAAATCATCGAGTTCTATCAATTCATTGAAGCAT
CACAATGCTGATGACAAAAAATCTGTCAACACTGTCATTGAATTGGCATC
AAGCAAAAGCCACACCCAGAGTAGCCGTTGTTCCAGCAGTTTCAGCTCTT
CATCAACGATCCGATACCATTGCCATTTGTGCTTAAAGACATTTCCTCGG
GCTGCCAACTTGAACCGACATTTGCGAACTCATACTGGTGAACAACCGTA
CAAGTGTGCTTTCTGCGACAAATGTTTCAGTATTTCTTCCAATATGCAGC
GCCATGTGAGAAACATTCACAATAAAGAGCGTCCGTACAAATGTACGAAG
TGCGATCGGATATTTGATCAATTGACTTCATTGGACCGGCACATTCGTCA
ACTGTGTAGCATGTGAAATCTATTAATACGAAATTTTCTG
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protein sequence of SMED30024013-orf-1

>SMED30024013-orf-1 ID=SMED30024013-orf-1|Name=SMED30024013-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=581bp
MNFAKLTGESQSGFLFTSGLMNGEKSQIPLPFFDSNWKDVNTNEWCSLKL
DHLGLNYQEKLENLPTFIASVANIYSKSTTQASSQFQCPDCGANFSSRGP
LKRHQNALCKNPNGLLIKTRQTIFKHNSVIQRKQVLGTNQNCTTTNLSGG
SEHRKTSSDYKQSVSFPISKLSSPISNGSNLNLFCDVCNKPCRSQVSLIR
HRKQHQNPNSHYSCDVCGKTFKISSGLKQHMHIHSSNKPYSCLNCQKSYT
QYSNLCRHKRMSRDCQRKAKCTECGMEFTNGKTYDTHKQHCLGLDPSGYL
KSPSYSSQANSINNNNNNSGNIIESTWSSPNVHIQQSATVKSFNTEDLIR
EIVPLDLTVRKSKTDCPTSQTFPDSRVEQLIKWNNLNKCGGNNSIQSLNP
NHLNSINSMASAFPSNCHGNDYMSSINQYFLQFPVMIPRPRSLNLDNSKS
SSSINSLKHHNADDKKSVNTVIELASSKSHTQSSRCSSSFSSSSTIRYHC
HLCLKTFPRAANLNRHLRTHTGEQPYKCAFCDKCFSISSNMQRHVRNIHN
KERPYKCTKCDRIFDQLTSLDRHIRQLCSM*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: INTERPRO
TermDefinition
IPR041697Znf-C2H2_11
IPR036236Znf_C2H2_sf
IPR015880Znf_C2H2-like
IPR013087Znf_C2H2_type
IPR007087Znf_C2H2
Vocabulary: molecular function
TermDefinition
GO:0003676nucleic acid binding
GO:0046872metal ion binding
GO:0003677DNA binding
Vocabulary: cellular component
TermDefinition
GO:0005622intracellular
GO:0005634nucleus
Vocabulary: biological process
TermDefinition
GO:0006351transcription, DNA-templated
GO:0006355regulation of transcription, DNA-templated
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013087Zinc finger C2H2-typeSMARTSM00355c2h2final6coord: 240..260
e-value: 60.0
score: 6.4
coord: 526..549
e-value: 0.0036
score: 26.5
coord: 86..106
e-value: 14.0
score: 12.0
coord: 269..290
e-value: 16.0
score: 11.4
coord: 555..575
e-value: 9.0
score: 13.7
coord: 212..234
e-value: 9.0E-4
score: 28.5
coord: 498..520
e-value: 8.8E-4
score: 28.6
coord: 183..205
e-value: 0.023
score: 23.8
IPR013087Zinc finger C2H2-typePFAMPF00096zf-C2H2coord: 212..234
e-value: 1.7E-4
score: 21.9
coord: 498..520
e-value: 8.3E-5
score: 22.8
coord: 526..549
e-value: 0.0023
score: 18.3
coord: 555..575
e-value: 0.006
score: 17.0
IPR013087Zinc finger C2H2-typePROSITEPS00028ZINC_FINGER_C2H2_1coord: 500..520
IPR013087Zinc finger C2H2-typePROSITEPS00028ZINC_FINGER_C2H2_1coord: 528..549
IPR013087Zinc finger C2H2-typePROSITEPS00028ZINC_FINGER_C2H2_1coord: 214..234
IPR013087Zinc finger C2H2-typePROSITEPS50157ZINC_FINGER_C2H2_2coord: 183..210
score: 8.6
IPR013087Zinc finger C2H2-typePROSITEPS50157ZINC_FINGER_C2H2_2coord: 555..580
score: 9.743
IPR013087Zinc finger C2H2-typePROSITEPS50157ZINC_FINGER_C2H2_2coord: 86..105
score: 9.432
IPR013087Zinc finger C2H2-typePROSITEPS50157ZINC_FINGER_C2H2_2coord: 498..525
score: 15.708
IPR013087Zinc finger C2H2-typePROSITEPS50157ZINC_FINGER_C2H2_2coord: 212..239
score: 15.147
IPR013087Zinc finger C2H2-typePROSITEPS50157ZINC_FINGER_C2H2_2coord: 240..268
score: 9.785
IPR013087Zinc finger C2H2-typePROSITEPS50157ZINC_FINGER_C2H2_2coord: 526..554
score: 14.627
NoneNo IPR availableGENE3DG3DSA:3.30.160.60coord: 522..548
e-value: 1.5E-12
score: 49.0
NoneNo IPR availableGENE3DG3DSA:3.30.160.60coord: 184..231
e-value: 2.4E-11
score: 45.8
NoneNo IPR availableGENE3DG3DSA:3.30.160.60coord: 232..295
e-value: 3.7E-9
score: 38.6
NoneNo IPR availablePFAMPF12874zf-metcoord: 184..201
e-value: 0.032
score: 14.6
NoneNo IPR availableGENE3DG3DSA:3.30.160.60coord: 497..521
e-value: 4.2E-9
score: 38.1
NoneNo IPR availableGENE3DG3DSA:3.30.160.60coord: 549..576
e-value: 6.2E-8
score: 34.4
NoneNo IPR availablePANTHERPTHR24393FAMILY NOT NAMEDcoord: 490..575
NoneNo IPR availablePANTHERPTHR24393FAMILY NOT NAMEDcoord: 202..293
NoneNo IPR availablePANTHERPTHR24393:SF69MDS1 AND EVI1 COMPLEX LOCUS PROTEINcoord: 202..293
coord: 490..575
IPR041697Zinc-finger C2H2-type 11PFAMPF16622zf-C2H2_11coord: 84..104
e-value: 3.6E-5
score: 23.4
IPR036236Zinc finger C2H2 superfamilySUPERFAMILYSSF57667beta-beta-alpha zinc fingerscoord: 86..205
IPR036236Zinc finger C2H2 superfamilySUPERFAMILYSSF57667beta-beta-alpha zinc fingerscoord: 209..258
IPR036236Zinc finger C2H2 superfamilySUPERFAMILYSSF57667beta-beta-alpha zinc fingerscoord: 506..564