SMED30023994

Overview
NameSMED30023994
Smed IDSMED30023994
Length (bp)1526
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30023994 (SMED30023994) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30023994 (SMED30023994) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30023994 (SMED30023994) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30023994 (SMED30023994) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30023994

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 5

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
cephalic gangliaSMED30023994SMESG000076399.1 dd_Smed_v4_5426_1_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30023994SMESG000076399.1 dd_Smed_v4_5426_1_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30023994SMESG000076399.1 dd_Smed_v4_5426_1_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30023994SMESG000076399.1 dd_Smed_v4_5426_1_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
enteric muscle cellSMED30023994SMESG000076399.1 dd_Smed_v4_5426_1_1dd_Smed_v4PMID:30471994
Scimone et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30023994 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.007453:3865..17587 +6945
Homology
[Close]
BLAST of SMED30023994 vs. Ensembl Fly
Match: M6 (gene:FBgn0037092 transcript:FBtr0332713)

HSP 1 Score: 50.8322 bits (120), Expect = 9.604e-7
Identity = 36/134 (26.87%), Postives = 69/134 (51.49%), Query Frame = 3 
Query:   90 CISQIPYTSVIGFISVMIGCAVTSATLYDSINRTDELLSKYF-FKINFLSKALVIFATLSXXXXXXXXXXXXXXXXXTLATRNKVYNRSRCILGGRRIAAMLMCLLFVANVTWVGIMCLLMIPVLGWVMLSTSC 488
            C+++IPY ++I  +  ++G  +   T+Y   + T  ++ + F  ++ ++    +IF  +   +  +   +L   F+AT ATR KVY   R  +GGR   A+LM + ++ N  W  I+C L++    + M    C
Sbjct:    8 CMARIPYATLIATLMCLLGVGIFCFTMYRGASLTVIMVDQVFHLRLIWIEAVQMIFVIIGAGMAALGFMILFVGFLATGATRYKVYRAWRSRVGGRISCAVLMGITYLLNFVWSLILCFLVVVTFIYTMFWNMC 141
[Close]
BLAST of SMED30023994 vs. Ensembl Fly
Match: M6 (gene:FBgn0037092 transcript:FBtr0332714)

HSP 1 Score: 50.447 bits (119), Expect = 1.967e-6
Identity = 36/134 (26.87%), Postives = 69/134 (51.49%), Query Frame = 3 
Query:   90 CISQIPYTSVIGFISVMIGCAVTSATLYDSINRTDELLSKYF-FKINFLSKALVIFATLSXXXXXXXXXXXXXXXXXTLATRNKVYNRSRCILGGRRIAAMLMCLLFVANVTWVGIMCLLMIPVLGWVMLSTSC 488
            C+++IPY ++I  +  ++G  +   T+Y   + T  ++ + F  ++ ++    +IF  +   +  +   +L   F+AT ATR KVY   R  +GGR   A+LM + ++ N  W  I+C L++    + M    C
Sbjct:   57 CMARIPYATLIATLMCLLGVGIFCFTMYRGASLTVIMVDQVFHLRLIWIEAVQMIFVIIGAGMAALGFMILFVGFLATGATRYKVYRAWRSRVGGRISCAVLMGITYLLNFVWSLILCFLVVVTFIYTMFWNMC 190
[Close]
BLAST of SMED30023994 vs. Ensembl Fly
Match: M6 (gene:FBgn0037092 transcript:FBtr0078356)

HSP 1 Score: 50.447 bits (119), Expect = 2.077e-6
Identity = 36/134 (26.87%), Postives = 69/134 (51.49%), Query Frame = 3 
Query:   90 CISQIPYTSVIGFISVMIGCAVTSATLYDSINRTDELLSKYF-FKINFLSKALVIFATLSXXXXXXXXXXXXXXXXXTLATRNKVYNRSRCILGGRRIAAMLMCLLFVANVTWVGIMCLLMIPVLGWVMLSTSC 488
            C+++IPY ++I  +  ++G  +   T+Y   + T  ++ + F  ++ ++    +IF  +   +  +   +L   F+AT ATR KVY   R  +GGR   A+LM + ++ N  W  I+C L++    + M    C
Sbjct:   74 CMARIPYATLIATLMCLLGVGIFCFTMYRGASLTVIMVDQVFHLRLIWIEAVQMIFVIIGAGMAALGFMILFVGFLATGATRYKVYRAWRSRVGGRISCAVLMGITYLLNFVWSLILCFLVVVTFIYTMFWNMC 207
[Close]
BLAST of SMED30023994 vs. Ensembl Fly
Match: M6 (gene:FBgn0037092 transcript:FBtr0339793)

HSP 1 Score: 48.521 bits (114), Expect = 2.462e-6
Identity = 36/135 (26.67%), Postives = 69/135 (51.11%), Query Frame = 3 
Query:   87 ECISQIPYTSVIGFISVMIGCAVTSATLYDSINRTDELLSKYF-FKINFLSKALVIFATLSXXXXXXXXXXXXXXXXXTLATRNKVYNRSRCILGGRRIAAMLMCLLFVANVTWVGIMCLLMIPVLGWVMLSTSC 488
             C+++IPY ++I  +  ++G  +   T+Y   + T  ++ + F  ++ ++    +IF  +   +  +   +L   F+AT ATR KVY   R  +GGR   A+LM + ++ N  W  I+C L++    + M    C
Sbjct:    7 SCMARIPYATLIATLMCLLGVGIFCFTMYRGASLTVIMVDQVFHLRLIWIEAVQMIFVIIGAGMAALGFMILFVGFLATGATRYKVYRAWRSRVGGRISCAVLMGITYLLNFVWSLILCFLVVVTFIYTMFWNMC 141
[Close]
BLAST of SMED30023994 vs. Ensembl Fly
Match: M6 (gene:FBgn0037092 transcript:FBtr0332712)

HSP 1 Score: 50.0618 bits (118), Expect = 2.581e-6
Identity = 36/134 (26.87%), Postives = 69/134 (51.49%), Query Frame = 3 
Query:   90 CISQIPYTSVIGFISVMIGCAVTSATLYDSINRTDELLSKYF-FKINFLSKALVIFATLSXXXXXXXXXXXXXXXXXTLATRNKVYNRSRCILGGRRIAAMLMCLLFVANVTWVGIMCLLMIPVLGWVMLSTSC 488
            C+++IPY ++I  +  ++G  +   T+Y   + T  ++ + F  ++ ++    +IF  +   +  +   +L   F+AT ATR KVY   R  +GGR   A+LM + ++ N  W  I+C L++    + M    C
Sbjct:   79 CMARIPYATLIATLMCLLGVGIFCFTMYRGASLTVIMVDQVFHLRLIWIEAVQMIFVIIGAGMAALGFMILFVGFLATGATRYKVYRAWRSRVGGRISCAVLMGITYLLNFVWSLILCFLVVVTFIYTMFWNMC 212
[Close]
BLAST of SMED30023994 vs. TrEMBL
Match: A0A1I8IK25 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig023149g1 PE=4 SV=1)

HSP 1 Score: 165.236 bits (417), Expect = 1.205e-43
Identity = 100/256 (39.06%), Postives = 152/256 (59.38%), Query Frame = 3 
Query:   87 ECISQIPYTSVIGFISVMIGCAVTSATLYDSINRTDELLSKYFFKINFLSKALVIFATLSXXXXXXXXXXXXXXXXXTLATRNKVYNRSRCILGGRRIAAMLMCLLFVANVTWVGIMCLLMIPVLGWVMLSTSCRYEISQYQQ-------TKQFSY-NFSLTNYGIYPDYWQPADSTEKVKLSITDFEMYKEFCQLISTIGPLYTCSAVGSLVVILGLITFIGSLSSMYMRLKYTKELTNFIVAFEDQKNNELDYI 830
            EC++ IPY SVI F+ V+IG  V + TLY+++ +TD      F+ I  L    +    +S V V++ V +L F  +AT ATRNK+Y   RCILGGR  A + M   ++ ++ WVGI C LMIP++G++M+   C  E + ++         K F+Y  F+LT+YG+Y   +       K   +IT    ++ FC  I TIGPLY  +  G++ VI GL+ FIG+LS+ + +LKYTKELT++  A   + + E+DY+
Sbjct:    3 ECLANIPYASVIAFVLVLIGGCVMTGTLYEAVRQTDVYFRSTFYPIEELLYVQIASVVVSPVTVVLAVILLTFGILATGATRNKIYRGDRCILGGRFSAGLFMAFSYLLSLVWVGIACFLMIPIVGYIMMDKVCYEEWTYWRDIDGEKTNAKFFTYFKFNLTHYGLYKKLYTFRPEL-KFNETITTATAFQNFCSKIGTIGPLYAFAWAGAVFVIFGLMCFIGALSANFTKLKYTKELTDYRAA--GEVSTEMDYL 255
[Close]
BLAST of SMED30023994 vs. TrEMBL
Match: A0A267G677 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig023149g2 PE=4 SV=1)

HSP 1 Score: 164.851 bits (416), Expect = 1.596e-43
Identity = 100/256 (39.06%), Postives = 152/256 (59.38%), Query Frame = 3 
Query:   87 ECISQIPYTSVIGFISVMIGCAVTSATLYDSINRTDELLSKYFFKINFLSKALVIFATLSXXXXXXXXXXXXXXXXXTLATRNKVYNRSRCILGGRRIAAMLMCLLFVANVTWVGIMCLLMIPVLGWVMLSTSCRYEISQYQQ-------TKQFSY-NFSLTNYGIYPDYWQPADSTEKVKLSITDFEMYKEFCQLISTIGPLYTCSAVGSLVVILGLITFIGSLSSMYMRLKYTKELTNFIVAFEDQKNNELDYI 830
            EC++ IPY SVI F+ V+IG  V + TLY+++ +TD      F+ I  L    +    +S V V++ V +L F  +AT ATRNK+Y   RCILGGR  A + M   ++ ++ WVGI C LMIP++G++M+   C  E + ++         K F+Y  F+LT+YG+Y   +       K   +IT    ++ FC  I TIGPLY  +  G++ VI GL+ FIG+LS+ + +LKYTKELT++  A   + + E+DY+
Sbjct:    3 ECLANIPYASVIAFVLVLIGGCVMTGTLYEAVRQTDVYFRSTFYPIEELLYVQIASVVVSPVTVVLAVILLTFGILATGATRNKIYRGDRCILGGRFSAGLFMAFSYLISLVWVGIACFLMIPIVGYIMMDKVCYEEWTYWRDIDGEKTNAKFFTYFKFNLTHYGLYKKLYTFRPEL-KFNETITTATAFQNFCSKIGTIGPLYAFAWAGAVFVIFGLMCFIGALSANFTKLKYTKELTDYRAA--GEVSTEMDYL 255
[Close]
BLAST of SMED30023994 vs. TrEMBL
Match: A0A267GF79 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig001072g2 PE=4 SV=1)

HSP 1 Score: 140.198 bits (352), Expect = 3.631e-34
Identity = 85/255 (33.33%), Postives = 147/255 (57.65%), Query Frame = 3 
Query:   93 ISQIPYTSVIGFISVMIGCAVTSATLYDSINRTDELLSKYFFKINFLSKALVIFATLSXXXXXXXXXXXXXXXXXTLATRNKVYNRSRCILGGRRIAAMLMCLLFVANVTWVGIMCLLMIPVLGWVMLSTSC----RYEIS---QYQQTKQFSY-NFSLTNYGIYPDYWQPADSTEKVKLSITDFEMYKEFCQLISTIGPLYTCSAVGSLVVILGLITFIGSLSSMYMRLKYTKELTNFIVAFEDQ-KNNELDYI 830
            +  IPY S+I F+ +++G  + + TLY+++ RTD      F+ I+  S A  +   ++ + V++   +L F  +AT ATR K+Y  +R IL GR  A + + L  ++ + W+ +MC LM P  GW+++   C    RY ++   +    K F+Y  F+LT+YGIY   +   + + K   +IT    ++ FC  ++TIGPLY  +  G++ V+ GL  FIG+LS+ Y +LKYT++LT +  A + + +  E +Y+
Sbjct:    5 LENIPYPSMISFVLILVGGCIMTGTLYEAVRRTDTYFRSTFYPIDGFSYAQTVSVIITPITVVLAAALLAFGCLATGATREKIYRGNRSILSGRGAALVFLALAHLSCLCWLALMCGLMFPATGWLVMDRVCAEEWRYWLNIDGEKTNAKFFNYFRFNLTHYGIYKKLYN-FNPSLKYNETITTATAFQNFCSNVNTIGPLYMFALGGAMFVLFGLFCFIGALSANYTKLKYTQDLTQYRTAKQPEVEPAETEYL 258
[Close]
BLAST of SMED30023994 vs. TrEMBL
Match: A0A267FYM8 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig001072g3 PE=4 SV=1)

HSP 1 Score: 140.198 bits (352), Expect = 3.782e-34
Identity = 84/255 (32.94%), Postives = 145/255 (56.86%), Query Frame = 3 
Query:   93 ISQIPYTSVIGFISVMIGCAVTSATLYDSINRTDELLSKYFFKINFLSKALVIFATLSXXXXXXXXXXXXXXXXXTLATRNKVYNRSRCILGGRRIAAMLMCLLFVANVTWVGIMCLLMIPVLGWVMLSTSCRYE-------ISQYQQTKQFSY-NFSLTNYGIYPDYWQPADSTEKVKLSITDFEMYKEFCQLISTIGPLYTCSAVGSLVVILGLITFIGSLSSMYMRLKYTKELTNFIVAFEDQ-KNNELDYI 830
            +  IPY S+I F+ +++G  + + TLY+++ RTD      F+ I+ LS A  +   ++ + V++   +L F  +AT ATR K+Y  ++ IL GR  A + + L  ++ + W+ +MC LM P  GW+++   C  E         +    K F+Y  F+LT+YGIY   +   + + K   +IT    ++ FC  ++TIGPLY  +  G++ V+ GL  FIG+LS+ Y +LKYT++LT +  A + + +  E +Y+
Sbjct:    5 LENIPYPSMISFVLILVGGCIMTGTLYEAVRRTDTYFRSTFYPIDGLSYAQTVSVIITPITVVLAAALLAFGCLATGATREKIYRGNKSILSGRGAALVFLALAHLSCLCWLALMCGLMFPATGWLVMDRVCAEEWRYWLDIDGEKTNAKFFNYFRFNLTHYGIYKKLYN-FNPSLKYNETITTATAFQNFCSNVNTIGPLYMFALGGAMFVLFGLFCFIGALSANYTKLKYTQDLTQYRTAKQPEVEPAETEYL 258
[Close]
BLAST of SMED30023994 vs. TrEMBL
Match: A0A1I8H8I2 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 140.198 bits (352), Expect = 3.821e-34
Identity = 84/255 (32.94%), Postives = 144/255 (56.47%), Query Frame = 3 
Query:   93 ISQIPYTSVIGFISVMIGCAVTSATLYDSINRTDELLSKYFFKINFLSKALVIFATLSXXXXXXXXXXXXXXXXXTLATRNKVYNRSRCILGGRRIAAMLMCLLFVANVTWVGIMCLLMIPVLGWVMLSTSCRYE-------ISQYQQTKQFSY-NFSLTNYGIYPDYWQPADSTEKVKLSITDFEMYKEFCQLISTIGPLYTCSAVGSLVVILGLITFIGSLSSMYMRLKYTKELTNFIVAFEDQ-KNNELDYI 830
            +  IPY S+I F+ +++G  + + TLY+++ RTD      F+ I+  S A  +   ++ + V++   +L F  +AT ATR K+Y  +R IL GR  A + + L  ++ + W+ +MC LM P  GW+++   C  E         +    K F+Y  F+LT+YGIY   +   + + K   +IT    ++ FC  ++TIGPLY  +  G++ V+ GL  FIG+LS+ Y +LKYT++LT +  A + + +  E +Y+
Sbjct:    5 LENIPYPSMISFVLILVGGCIMTGTLYEAVRRTDTYFRSTFYPIDGFSYAQTVSVIITPITVVLAAALLAFGCLATGATREKIYRGNRSILSGRGAALVFLALAHLSCLCWLALMCGLMFPATGWLVMDRVCAEEWRYWLDIDGEKTNAKFFNYFRFNLTHYGIYKKLYN-FNPSLKYNETITTATAFQNFCSNVNTIGPLYMFALGGAMFVLFGLFCFIGALSANYTKLKYTQDLTQYRTAKQPEVEPAETEYL 258
[Close]
BLAST of SMED30023994 vs. Planmine SMEST
Match: SMESG000076399.1 (SMESG000076399.1)

HSP 1 Score: 481.871 bits (1239), Expect = 1.276e-170
Identity = 254/255 (99.61%), Postives = 255/255 (100.00%), Query Frame = 3 
Query:   84 MECISQIPYTSVIGFISVMIGCAVTSATLYDSINRTDELLSKYFFKINFLSKALVIFATLSXXXXXXXXXXXXXXXXXTLATRNKVYNRSRCILGGRRIAAMLMCLLFVANVTWVGIMCLLMIPVLGWVMLSTSCRYEISQYQQTKQFSYNFSLTNYGIYPDYWQPADSTEKVKLSITDFEMYKEFCQLISTIGPLYTCSAVGSLVVILGLITFIGSLSSMYMRLKYTKELTNFIVAFEDQKNNELDYIVNTKAM 848
            MECISQIPYTSVIGFISVMIGCAVTSATLYDSINRTDELLSKYFFKINFLSKALVIFATLSIVLVIICVFVLIFAFIATLATRNKVYNRSRCILGGRRIAAMLMCLLFVANVTWVGIMCLLMIPVLGWVMLSTSCRYEISQYQQTKQFSYNFSL+NYGIYPDYWQPADSTEKVKLSITDFEMYKEFCQLISTIGPLYTCSAVGSLVVILGLITFIGSLSSMYMRLKYTKELTNFIVAFEDQKNNELDYIVNTKAM
Sbjct:    1 MECISQIPYTSVIGFISVMIGCAVTSATLYDSINRTDELLSKYFFKINFLSKALVIFATLSIVLVIICVFVLIFAFIATLATRNKVYNRSRCILGGRRIAAMLMCLLFVANVTWVGIMCLLMIPVLGWVMLSTSCRYEISQYQQTKQFSYNFSLSNYGIYPDYWQPADSTEKVKLSITDFEMYKEFCQLISTIGPLYTCSAVGSLVVILGLITFIGSLSSMYMRLKYTKELTNFIVAFEDQKNNELDYIVNTKAM 255
[Close]
BLAST of SMED30023994 vs. Planmine SMEST
Match: SMESG000033520.1 (SMESG000033520.1)

HSP 1 Score: 86.6557 bits (213), Expect = 1.531e-19
Identity = 53/164 (32.32%), Postives = 91/164 (55.49%), Query Frame = 3 
Query:  324 ATRNKVYNRSRCILGGRRIAAMLMCLLFVANVTWVGIMCLLMIPVLGWVMLSTSCRYEISQYQQTKQFSYNFSLTNYGIYPDYWQPADSTEKVKLSITDFEMYKEFCQLISTIGPLYTCSAVGSLVVILGLITFIGSLSSMYMRLKYTKELTNF--IVAFEDQK 809
            AT++ VY      +GG+  A  L+ + F A + W+ I   L+IP + W+MLS++C +E+        F + F+LT++G Y + ++  D +   + +IT    ++  C   +  GPL+  +  GSL+V+LGL  F   LS+ Y RL++T E+  +  IV  E +K
Sbjct:   22 ATKSSVYRGDFMCIGGKNSATALLAVNFTAVLIWIIISSFLVIPSIIWLMLSSTCLHEL---LPGLAFKFIFNLTSFGFYEEPYK-YDVSLNYREAITTRSDFESLCNENTKSGPLFVGALGGSLLVVLGLALFNTYLSASYARLRFTNEIAEYRKIVDLEKEK 181
[Close]
BLAST of SMED30023994 vs. Planmine SMEST
Match: SMESG000033520.1 (SMESG000033520.1)

HSP 1 Score: 86.2705 bits (212), Expect = 5.655e-19
Identity = 53/164 (32.32%), Postives = 91/164 (55.49%), Query Frame = 3 
Query:  324 ATRNKVYNRSRCILGGRRIAAMLMCLLFVANVTWVGIMCLLMIPVLGWVMLSTSCRYEISQYQQTKQFSYNFSLTNYGIYPDYWQPADSTEKVKLSITDFEMYKEFCQLISTIGPLYTCSAVGSLVVILGLITFIGSLSSMYMRLKYTKELTNF--IVAFEDQK 809
            AT++ VY      +GG+  A  L+ + F A + W+ I   L+IP + W+MLS++C +E+        F + F+LT++G Y + ++  D +   + +IT    ++  C   +  GPL+  +  GSL+V+LGL  F   LS+ Y RL++T E+  +  IV  E +K
Sbjct:   57 ATKSSVYRGDFMCIGGKNSATALLAVNFTAVLIWIIISSFLVIPSIIWLMLSSTCLHEL---LPGLAFKFIFNLTSFGFYEEPYK-YDVSLNYREAITTRSDFESLCNENTKSGPLFVGALGGSLLVVLGLALFNTYLSASYARLRFTNEIAEYRKIVDLEKEK 216
The following BLAST results are available for this feature:
BLAST of SMED30023994 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023994 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023994 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
M69.604e-726.87gene:FBgn0037092 transcript:FBtr0332713[more]
M61.967e-626.87gene:FBgn0037092 transcript:FBtr0332714[more]
M62.077e-626.87gene:FBgn0037092 transcript:FBtr0078356[more]
M62.462e-626.67gene:FBgn0037092 transcript:FBtr0339793[more]
M62.581e-626.87gene:FBgn0037092 transcript:FBtr0332712[more]
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BLAST of SMED30023994 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023994 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023994 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023994 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023994 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A1I8IK251.205e-4339.06Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A267G6771.596e-4339.06Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A267GF793.631e-3433.33Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A267FYM83.782e-3432.94Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A1I8H8I23.821e-3432.94Uncharacterized protein OS=Macrostomum lignano OX=... [more]
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BLAST of SMED30023994 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023994 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023994 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023994 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023994 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023994 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 3
Match NameE-valueIdentityDescription
SMESG000076399.11.276e-17099.61SMESG000076399.1[more]
SMESG000033520.11.531e-1932.32SMESG000033520.1[more]
SMESG000033520.15.655e-1932.32SMESG000033520.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30023994 ID=SMED30023994|Name=SMED30023994|organism=Schmidtea mediterranea sexual|type=transcript|length=1526bp
GAAAATATTAAATTTTAAATTTTATTTTTAATTTCTCTAATTTGTAATAT
TTTAAAAAGTTCTGAGAACTCTCGACAATCTAAATGGAATGTATTAGTCA
AATTCCATACACATCAGTTATTGGATTTATTTCTGTTATGATCGGATGTG
CAGTTACAAGTGCGACGCTATATGATTCGATTAATAGAACTGATGAGCTA
TTATCCAAATATTTTTTCAAAATCAACTTCCTTAGTAAAGCTTTAGTAAT
TTTTGCAACTCTGTCGATTGTTTTGGTGATTATTTGTGTTTTTGTACTGA
TTTTTGCATTTATCGCCACTCTGGCTACAAGAAATAAAGTTTATAACAGA
TCGAGATGCATTTTAGGTGGCCGAAGGATAGCAGCAATGTTGATGTGCTT
GCTGTTTGTTGCCAATGTTACCTGGGTTGGTATAATGTGCCTGTTGATGA
TCCCGGTACTCGGATGGGTTATGCTGAGTACGAGTTGTCGTTATGAGATC
TCTCAGTATCAGCAAACGAAGCAATTCTCTTACAATTTCAGCCTGACAAA
TTACGGCATTTATCCAGATTATTGGCAGCCAGCTGACAGCACAGAAAAAG
TTAAACTTTCTATCACCGATTTTGAAATGTACAAAGAATTTTGTCAATTG
ATCTCTACAATCGGGCCGCTCTACACTTGCAGTGCAGTTGGATCCTTGGT
CGTCATTCTGGGATTAATCACATTCATAGGATCTCTCTCATCGATGTATA
TGCGCCTCAAATACACAAAAGAGTTGACTAACTTCATCGTGGCATTTGAG
GACCAAAAGAACAACGAGCTCGATTACATCGTCAACACAAAGGCCATGTG
ACCGAAACTGCAAATAAATATTGACATTAGCATGTATCTTTTTAATAAAT
ATTCCCTTCCTGTCGCTGTCGAGTGATTTGCCGGGTTTCCTTGTTGTTTC
TTATTTCCGTTTGTGTAGATATTTTGATCTAGCAATTCTGTACAGCCCCG
CATGAGATATATTGTTTTGTAATTTTATGTCTATTGTGATTCGATTTATG
CAGTGCAATTTGCTGCTGCTGCATCTTATTGTATTCTTTCTGACTCCGTG
CTATTCACCTCGCCCGACTTTTTTACTCGTTCGACTTGTCAATTATTTAT
ATTCATTCATTTGTTTATTTCTCTGTGCAATATGTTGTTTTAATTTCTTT
ATAATATAACTTGTTGGATTGTTATTGCGTTTTGTTGATACTGATATATA
ACCCGTCATGTTCTGCATCATTTCATTGTTTGCATTGATCATTCATTGCG
TCACAGTTCATGGCTGCGTTCATATTGCTGCGTGATTAAATGATATTCAA
TCATCTGGTGACGTAATACTCATATGCCAGATGATTGCTGTAGTTTTGTG
TAGACATAATACAGTTGAATCCTGCATACAAGCTTGAGAAAACCCTAATG
GTAAGTTTTCAGTTTTTAAATAATCAATTTTTTGCACAATACATTGGAAT
CAATATTTTTTCCCAATAAATCTTTG
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protein sequence of SMED30023994-orf-1

>SMED30023994-orf-1 ID=SMED30023994-orf-1|Name=SMED30023994-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=256bp
MECISQIPYTSVIGFISVMIGCAVTSATLYDSINRTDELLSKYFFKINFL
SKALVIFATLSIVLVIICVFVLIFAFIATLATRNKVYNRSRCILGGRRIA
AMLMCLLFVANVTWVGIMCLLMIPVLGWVMLSTSCRYEISQYQQTKQFSY
NFSLTNYGIYPDYWQPADSTEKVKLSITDFEMYKEFCQLISTIGPLYTCS
AVGSLVVILGLITFIGSLSSMYMRLKYTKELTNFIVAFEDQKNNELDYIV
NTKAM*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000101muscle cell
PLANA:0003104enteric muscle cell
PLANA:0003116parenchymal cell
Vocabulary: INTERPRO
TermDefinition
IPR001614Myelin_PLP
Vocabulary: cellular component
TermDefinition
GO:0016021integral component of membrane
GO:0016020membrane
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001614Myelin proteolipid protein PLPPFAMPF01275Myelin_PLPcoord: 1..228
e-value: 8.8E-23
score: 81.4
IPR001614Myelin proteolipid protein PLPPANTHERPTHR11683MYELIN PROTEOLIPIDcoord: 2..245
NoneNo IPR availablePANTHERPTHR11683:SF12M6, ISOFORM Fcoord: 2..245
NoneNo IPR availableTMHMMTMhelixcoord: 7..29
NoneNo IPR availableTMHMMTMhelixcoord: 196..218
NoneNo IPR availableTMHMMTMhelixcoord: 56..78
NoneNo IPR availableTMHMMTMhelixcoord: 99..121