SMED30023987

Overview
NameSMED30023987
Smed IDSMED30023987
Length (bp)3660
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30023987 (SMED30023987) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30023987 (SMED30023987) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30023987 (SMED30023987) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30023987 (SMED30023987) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30023987

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 20

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
Smed sexual biotypeSMED30023987h1SMcG0005825 Contig14157uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30023987h1SMcG0005825 Contig14157newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30023987h1SMcG0003217 Contig4616GPL15192PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30023987h1SMcG0005825 Contig4616GPL15192PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30023987h1SMcG0005825 Contig21206newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30023987h1SMcG0005825 Contig21206uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30023987h1SMcG0005825 Contig13466uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30023987h1SMcG0005825 Contig13466newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30023987h1SMcG0005825 Contig13053uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30023987h1SMcG0005825 Contig13053newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
cephalic gangliaSMED30023987h1SMcG0005825 dd_Smed_v4_16935_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30023987h1SMcG0005825 dd_Smed_v4_6644_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30023987h1SMcG0005825 dd_Smed_v4_6644_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30023987h1SMcG0005825 dd_Smed_v4_6644_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30023987h1SMcG0005825 dd_Smed_v4_6644_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30023987h1SMcG0005825 dd_Smed_v4_6644_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30023987h1SMcG0005828 dd_Smed_v4_16935_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
reproductive organSMED30023987h1SMcG0005825 Contig6998newmark_estsPMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
reproductive organSMED30023987h1SMcG0005825 Contig6998uc_Smed_v2PMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
epidermisSMED30023987h1SMcG0005825 dd_Smed_v4_6644_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of SMED30023987 vs. TrEMBL
Match: A0A553MUV9 (USP domain-containing protein (Fragment) OS=Danionella translucida OX=623744 GN=DNTS_023892 PE=4 SV=1)

HSP 1 Score: 66.2402 bits (160), Expect = 1.945e-7
Identity = 67/296 (22.64%), Postives = 123/296 (41.55%), Query Frame = 3 
Query:  108 GFPMPQSYCYLNSVLQMFFSCKIFRLNLSEWIKSNQNINENTNFLYALEDLFKSMESRRENNAQFAIHELQNVFQTLSSEEDIHKCYLQILSRIDAAVTRCSTAPENIGDQNEFPVNIKTKNDESLIDLFGGRKCLVKKCVSCGKTHEKLQRFKSMPLEVPSSPWKISIEHCIFNHFRDLAIMKSE------LSCCEYPNIKLSSQYAVVSQYIFIYLVREAFG---MSEKKIHTFIYFWSFIDIPTKDGNVKYKVISVIDHEDGLENGHYYLKALHXXXXXXKWLLKKNSQVVEI 968
            G P   + CYLN+VLQM F  + FR  +  +   +  +++      A++ LF++++   E +       LQ++   L          + +  + DAA               ++  NI  K D+ L  +F G       C+   KTH+ L++ K  P  + S      +    F+ + D  IM  E        C +  + K+++    V   + ++L R  F    M   K    +     + +  +DGNV +++ ++ +H   LE GHYY    H   +   W    +S+VV++
Sbjct:   22 GLPNRGATCYLNTVLQMLFMTERFREAVERFNGCDSPLSK------AIQVLFETLKVENETSTNKITDALQDIIDELK---------INVCKQADAA---------------KYLQNILRKLDKDLSGIFQGEMIQTTSCLDPEKTHDPLKQVK--PFFMISVSLNSDLVQECFDSYFDPIIMSGEGRQLSCRGCGKMMDTKMTTCLLKVPSVLVLHLERFEFDYEYMRCNKDMREVLVPPQLSVKMEDGNVLFELYAIANHNGSLEWGHYY---AHIKNADGHWFEFNDSKVVKV 282
[Close]
BLAST of SMED30023987 vs. TrEMBL
Match: A0A553MUW0 (USP domain-containing protein (Fragment) OS=Danionella translucida OX=623744 GN=DNTS_023892 PE=4 SV=1)

HSP 1 Score: 65.855 bits (159), Expect = 2.734e-7
Identity = 67/296 (22.64%), Postives = 123/296 (41.55%), Query Frame = 3 
Query:  108 GFPMPQSYCYLNSVLQMFFSCKIFRLNLSEWIKSNQNINENTNFLYALEDLFKSMESRRENNAQFAIHELQNVFQTLSSEEDIHKCYLQILSRIDAAVTRCSTAPENIGDQNEFPVNIKTKNDESLIDLFGGRKCLVKKCVSCGKTHEKLQRFKSMPLEVPSSPWKISIEHCIFNHFRDLAIMKSE------LSCCEYPNIKLSSQYAVVSQYIFIYLVREAFG---MSEKKIHTFIYFWSFIDIPTKDGNVKYKVISVIDHEDGLENGHYYLKALHXXXXXXKWLLKKNSQVVEI 968
            G P   + CYLN+VLQM F  + FR  +  +   +  +++      A++ LF++++   E +       LQ++   L          + +  + DAA               ++  NI  K D+ L  +F G       C+   KTH+ L++ K  P  + S      +    F+ + D  IM  E        C +  + K+++    V   + ++L R  F    M   K    +     + +  +DGNV +++ ++ +H   LE GHYY    H   +   W    +S+VV++
Sbjct:   22 GLPNRGATCYLNTVLQMLFMTERFREAVERFNGCDSPLSK------AIQVLFETLKVENETSTNKITDALQDIIDELK---------INVCKQADAA---------------KYLQNILRKLDKDLSGIFQGEMIQTTSCLDPEKTHDPLKQVK--PFFMISVSLNSDLVQECFDSYFDPIIMSGEGRQLSCRGCGKMMDTKMTTCLLKVPSVLVLHLERFEFDYEYMRCNKDMREVLVPPQLSVKMEDGNVLFELYAIANHNGSLEWGHYY---AHIKNADGHWFEFNDSKVVKV 282
[Close]
BLAST of SMED30023987 vs. TrEMBL
Match: A0CTQ8 (Uncharacterized protein OS=Paramecium tetraurelia OX=5888 GN=GSPATT00010409001 PE=3 SV=1)

HSP 1 Score: 65.0846 bits (157), Expect = 7.974e-7
Identity = 70/277 (25.27%), Postives = 120/277 (43.32%), Query Frame = 3 
Query:  105 IGFPMPQSYCYLNSVLQMFFSCKIFRLNLSEWIKSNQNINENTNFLYALEDLFKSMESRRENNAQFAIHELQ----NVFQTLSSEEDIHKCYLQILSRIDAA-VTRCSTAPENIGDQNEFPVNIKTKNDESLIDLFGGRKCLVKKCVSCGKTHEKLQRF--KSMPLEVPSSPWKISIEHCIFNHFRDLAIMKS-ELSCCEYPN--IKLSSQYAVVSQYIFIYLVREAFGMSEKKIHTFIYFWSFIDI----PTKDGNVKYKVISVIDHEDGLENGHY 893
            +G   P+ YCY+NS LQ   S  I  LN S   +S Q   +N  F  A +DL + +++    +A  ++ +LQ    N F+  S ++D H+  L +LS I    V +     E   +  E     KT+N + +  LF G+      C+ C +  E        ++PL     P +  ++ C+  +F++  I  + +   C++ N  +    Q     +Y+ ++L R       +KI   + +   +DI           KY +  VI H   L  GHY
Sbjct:  278 VGLQNPKYYCYMNSALQFLLS--IRELNDSLLKQSKQ---QNKKFTMAYQDLLRIVQNSIPGSA-ISVEKLQTMCLNKFK-YSQQQDAHEFLLYLLSEIQQELVGKNKYQKEEFQNAQEAWDVYKTRNPDIITSLFAGQIASKSHCLKCKEISEGFDPILDLNLPLSKYYIPREFKLQDCLQTYFKEEQINDAWKCDKCQFVNKSVLRKIQITQTPKYLILHLKRFTQFPKSQKITDEVTYPEILDIKEFCAENVEQTKYTLKGVISHMGQLNGGHY 547
[Close]
BLAST of SMED30023987 vs. TrEMBL
Match: A0CEF3 (Uncharacterized protein OS=Paramecium tetraurelia OX=5888 GN=GSPATT00037607001 PE=3 SV=1)

HSP 1 Score: 61.6178 bits (148), Expect = 9.701e-6
Identity = 70/281 (24.91%), Postives = 126/281 (44.84%), Query Frame = 3 
Query:  105 IGFPMPQSYCYLNSVLQMFFSCKIFRLNLSEWIKSNQNINENTNFLYALEDLFKSMESRRENNAQFAIHELQNV----FQTLSSEEDIHKCYLQILSRI-DAAVTRCSTAPENIGDQNEFPVNIKTKNDESLIDLFGGRKCLVKKCVSCGKTHEKLQRF--KSMPLEVPSSPWKISIEHCIFNHFRDLAIMKS-ELSCCEYPN------IKLSSQYAVVSQYIFIYLVREAFGMSEKKIHTFIYFWSFIDIPTK-DGNV---KYKVISVIDHEDGLENGHY 893
            +G   P+ YCY+NS+LQ   S K    +L ++ K      +N  F  A ++  K +++    ++  ++ +LQN+    F+  + ++D H+  L +LS I +  V +     E   +  E     K++N + + DLF G+      C  C +  E        ++PL+  S P +  +  C+ N+F++  I    +   C + N      IKL+       +Y+ ++L R       +KI   + +   +DI    + NV   KY +  VI H   L  GHY
Sbjct:  266 VGLNNPKYYCYMNSILQCLLSIKELNNSLIKYPKQ-----QNKKFTMAYQEFLKVVQNSIPGSS-ISVEKLQNMCLNKFK-YTQQQDAHEFLLYLLSEIQEELVGKKQYKKEEFPNAQEAWDIYKSRNPDIITDLFAGQIASKSYCSKCKQISEGFDPILDLNLPLQKNSIPKEYKLYDCLQNYFKEEQINDVWKCDRCNFVNKSVLRKIKLTQ----TPKYLILHLKRFTQIPRSQKITDEVKYPEILDIKEFCEENVEYTKYTLKGVISHMGQLNGGHY 535
[Close]
BLAST of SMED30023987 vs. TrEMBL
Match: A0EB12 (Uncharacterized protein OS=Paramecium tetraurelia OX=5888 GN=GSPATT00025213001 PE=3 SV=1)

HSP 1 Score: 61.6178 bits (148), Expect = 9.901e-6
Identity = 69/318 (21.70%), Postives = 135/318 (42.45%), Query Frame = 3 
Query:  105 IGFPMPQSYCYLNSVLQMFFSCKIFRLNLSEWIKSNQNINENTNFLYALEDLFKSMESRRENNAQFAIHELQNVFQTLSS------EEDIHKCYLQILSRIDAAVTRCSTAPENIGDQNEFPVNIKTKNDESLIDLFGGRKCLVKKCVSCGKTHEKLQRFKSMPLEVPSSPWKISIEHCIFNHFRDLAIMKSELSC--CEYPNIKLSSQYAV--VSQYIFIYLVREAFGMSEKKIHTFIYFWSFIDIP---TKDGNVKYKVISVIDHEDGLENGHYYLKALHXXXXXXKWLLKKNSQVVEIQDSEVCKRFSIGILLEK 1019
            +G   P+ YCYLNS LQ+  S      +LSE I + Q   +   +L A ++L +++    + +  F+    Q+V++  S       ++D H+  L  L +I   +       +      +   +  +KN + +  +F G+      C SC +  E       + L +  S   + +  C+ +++R+  ++     C  C+  N  +  +  +    +Y+ I   R     + +KI+  I +   +DI    +K    KYK+ ++I H   +  GHY   A    E    W L  +  V +I   ++  R +  +L +K
Sbjct:  267 VGLRNPRYYCYLNSALQVLLSID----SLSEGILAVQP-KQGQKYLVAYQELLRTI----KRSGSFSAIAAQSVWENCSGKFQFNQQQDSHEFLLFFLGKIQEELVGKYKNKQEFNSAEQAWSDYTSKNYDIVDTIFAGQSTSQSFCKSCNQVCEGYDPIWDLSLPISKSYTGVDLMDCLKSYYRE-EVINDTWKCDKCKKTNKSVKRRMFISQTPKYLIIQFKRFTTFPTSQKINDSISYPEKLDIQEFCSKGLQTKYKLKALITHMGSINGGHYKAYA----ERYDNWYLFDDETVSKINGKQLSDRSAYVLLYQK 570
[Close]
BLAST of SMED30023987 vs. Planmine SMEST
Match: SMESG000038485.1 (SMESG000038485.1)

HSP 1 Score: 969.148 bits (2504), Expect = 0.000e+0
Identity = 519/599 (86.64%), Postives = 529/599 (88.31%), Query Frame = 3 
Query:  348 EDIHKCYLQILSRIDAAVTRCSTAPENIGDQNEFPVNIKTKNDESLIDLFGGRKCLVKKCVSCGKTHEKLQRFKSMPLEVPSSPWKISIEHCIFNHFRDLAIMKSELSCCEYPNIKLSSQYAVVSQYIFIYLVREAFGMSEKKIHTFIYFWSFIDIPTKDGNVKYKVISVIDHEDGLENGHYYLKALHXXXXXXKWLLKKNSQVVEIQDSEVCKRFSIGILLEKAENGIQDVPKLLTSGKEFNSHFRSAMQAIFRIFYLCEDFKNGLISFYEKEIVNRNTLIYGTRLGEILHYMSENNNKLVDILIEEFCFNWSNPFYSIKELCCDILQKVNFNIKKAKEIRENPKDNNYNDTEKSNSEKFQNKPKAEAPXXXXXXXXXXXXXXXXXXXPKNFKSNKRNKNTERCQNKNNLNKDNYQKGCDTPMKRECKEKFKETSEQANDVKQIKEQIYSDNXXXXXXXXXXXXXXXXDNCNGKIFELLGFKIVDFTFPEEKYFFSTEKHYLQIKKCPKTEKSQFIPKYLFLCFSVNENHAEINQGSISQAQFEERVYSLLSIVNYDQSNGQNKYYVKCVEKSAELSGDFESISKSDINSNTLVAALM 2144
            EDIHKCYLQILSRIDAAVTRCSTAPENIGDQNEFPVNIKTKNDESLI+LFGGRKCL+KKCVSCGKTHEK QRFKSMPLEVPSSPWKISIEHCIFNHFRD AIMKSELSCCEYPNIKLSSQY  V QYIFIYLVREAFGMSEKKIHTFIYF SFIDIPTKDGNVKYKVISVIDHEDGLENGHYYLKALHESESSSKWLLKKNSQVVEIQDSEVCKRFSIGILLEKAENGIQDVPKLLTSGKEFNSHFRSAMQAIFRIFYLCEDFKN LISFYEKEIVNRNTLIYGT            +NK + I             +SIKELCCDILQKVNFNIKKA+EIRENPKDNNYN +                                  CFPKNFKSNKRNKNTERCQNKNNLNKDNYQKGCDTPMKRECKEKFKETSEQANDVKQIKEQIYSDNEKEQPEPEIIPIIPEIDNCNGKIFELLGFKIVDFTFPEEKYFFSTEKHYLQIKKCPKTEKSQFIPKYLFLCFSVNENHAEINQGSISQAQFEERVYSLLSIVNYDQSNG+NKYYVKCVEKSA+LSGDFESISKSDI +NTLVAALM
Sbjct:    4 EDIHKCYLQILSRIDAAVTRCSTAPENIGDQNEFPVNIKTKNDESLIELFGGRKCLLKKCVSCGKTHEKFQRFKSMPLEVPSSPWKISIEHCIFNHFRDSAIMKSELSCCEYPNIKLSSQYDAVPQYIFIYLVREAFGMSEKKIHTFIYFRSFIDIPTKDGNVKYKVISVIDHEDGLENGHYYLKALHESESSSKWLLKKNSQVVEIQDSEVCKRFSIGILLEKAENGIQDVPKLLTSGKEFNSHFRSAMQAIFRIFYLCEDFKNDLISFYEKEIVNRNTLIYGT------------SNKNIYI-------------FSIKELCCDILQKVNFNIKKAQEIRENPKDNNYNGS----------------------------------CFPKNFKSNKRNKNTERCQNKNNLNKDNYQKGCDTPMKRECKEKFKETSEQANDVKQIKEQIYSDNEKEQPEPEIIPIIPEIDNCNGKIFELLGFKIVDFTFPEEKYFFSTEKHYLQIKKCPKTEKSQFIPKYLFLCFSVNENHAEINQGSISQAQFEERVYSLLSIVNYDQSNGRNKYYVKCVEKSAQLSGDFESISKSDIKTNTLVAALM 543

HSP 2 Score: 369.392 bits (947), Expect = 4.671e-167
Identity = 180/194 (92.78%), Postives = 187/194 (96.39%), Query Frame = 3 
Query: 2577 KNLEGLQLAHLVRIILKSDEKTCYSSIISLLFLCPKFRDFVLEIYKKTGDVEIGKNSEMREKSPFFEICSLYHSLVSCGTLGDEYPSSEEKCTKNIFSEELGECYIALLQNLKKVFLPKFMKISDDDSVTGYSRLIHDLFGNKIVIKTSCLNCGLRTESKEFVYYLSIHLPIDKKSESIQNCVNNGFIGNVFKI 3158
            ++++ LQLAHLVRIILKSDEKTCYSSIISLLFLCPKFRDFVLEIYKKT   ++GK SEM  KSPFFEICSLYHSLVSCGTLGDEYPSSEEKCTKNIFSEELGECYIALLQNLKKVFLPKFMKISDDDSVTGYSRLIHDLFGNKIVIKTSCLNCGLRTESKEFVYYLSIHLPIDKKSESIQNCVNNGFIGNVFK+
Sbjct:  622 EDIKRLQLAHLVRIILKSDEKTCYSSIISLLFLCPKFRDFVLEIYKKTDSAKMGKYSEMPAKSPFFEICSLYHSLVSCGTLGDEYPSSEEKCTKNIFSEELGECYIALLQNLKKVFLPKFMKISDDDSVTGYSRLIHDLFGNKIVIKTSCLNCGLRTESKEFVYYLSIHLPIDKKSESIQNCVNNGFIGNVFKV 815

HSP 3 Score: 240.35 bits (612), Expect = 4.671e-167
Identity = 118/121 (97.52%), Postives = 120/121 (99.17%), Query Frame = 2 
Query: 3152 QNNCCSKQSHSTKTKFNCCGEYLLICFDKKTRERSKSGEINMKLDDGLLLEYIKSLDNKSTQVYSLYAVIDEIQSNSGKRYEIKFLSNEKWLRVNSTEAVPVDNNAITSLENAAFLFKKVT 3514
            ++NCCSKQSHSTKTKFNCCGEYLLICFDKKTRERSKSGEINMKLDDGLLLEYIKSLDNKSTQVYSLYAVIDEIQSNSGKRYEIKFLSNEKWLRVNSTEAV VDNNAITSLENAAFLFKKVT
Sbjct:  816 KDNCCSKQSHSTKTKFNCCGEYLLICFDKKTRERSKSGEINMKLDDGLLLEYIKSLDNKSTQVYSLYAVIDEIQSNSGKRYEIKFLSNEKWLRVNSTEAVSVDNNAITSLENAAFLFKKVT 936
[Close]
BLAST of SMED30023987 vs. Planmine SMEST
Match: SMESG000038485.1 (SMESG000038485.1)

HSP 1 Score: 964.14 bits (2491), Expect = 0.000e+0
Identity = 515/599 (85.98%), Postives = 523/599 (87.31%), Query Frame = 3 
Query:  348 EDIHKCYLQILSRIDAAVTRCSTAPENIGDQNEFPVNIKTKNDESLIDLFGGRKCLVKKCVSCGKTHEKLQRFKSMPLEVPSSPWKISIEHCIFNHFRDLAIMKSELSCCEYPNIKLSSQYAVVSQYIFIYLVREAFGMSEKKIHTFIYFWSFIDIPTKDGNVKYKVISVIDHEDGLENGHYYLKALHXXXXXXKWLLKKNSQVVEIQDSEVCKRFSIGILLEKAENGIQDVPKLLTSGKEFNSHFRSAMQAIFRIFYLCEDFKNGLISFYEKEIVNRNTLIYGTRLGEILHYMSENNNKLVDILIEEFCFNWSNPFYSIKELCCDILQKVNFNIKKAKEIRENPKDNNYNDTEKSNSEKFQNKPKAEAPXXXXXXXXXXXXXXXXXXXPKNFKSNKRNKNTERCQNKNNLNKDNYQKGCDTPMKRECKEKFKETSEQANDVKQIKEQIYSDNXXXXXXXXXXXXXXXXDNCNGKIFELLGFKIVDFTFPEEKYFFSTEKHYLQIKKCPKTEKSQFIPKYLFLCFSVNENHAEINQGSISQAQFEERVYSLLSIVNYDQSNGQNKYYVKCVEKSAELSGDFESISKSDINSNTLVAALM 2144
            EDIHKCYLQILSRIDAAVTRCSTAPENIGDQNEFPVNIKTKNDESLI+LFGGRKCL+KKCVSCGKTHEK QRFKSMPLEVPSSPWKISIEHCIFNHFRD AIMKSELSCCEYPNIKLSSQY  V QYIFIYLVREAFGMSEKKIHTFIYF SFIDIPTKDGNVKYKVISVIDHEDGLENGHYYLKALHESESSSKWLLKKNSQVVEIQDSEVCKRFSIGILLEKAENGIQDVPKLLTSGKEFNSHFRSAMQAIFRIFYLCEDFKN LISFYEKEIVNRNTLIYGT                                 +IKELCCDILQKVNFNIKKA+EIRENPKDNNYN +                                  CFPKNFKSNKRNKNTERCQNKNNLNKDNYQKGCDTPMKRECKEKFKETSEQANDVKQIKEQIYSDNEKEQPEPEIIPIIPEIDNCNGKIFELLGFKIVDFTFPEEKYFFSTEKHYLQIKKCPKTEKSQFIPKYLFLCFSVNENHAEINQGSISQAQFEERVYSLLSIVNYDQSNG+NKYYVKCVEKSA+LSGDFESISKSDI +NTLVAALM
Sbjct:    4 EDIHKCYLQILSRIDAAVTRCSTAPENIGDQNEFPVNIKTKNDESLIELFGGRKCLLKKCVSCGKTHEKFQRFKSMPLEVPSSPWKISIEHCIFNHFRDSAIMKSELSCCEYPNIKLSSQYDAVPQYIFIYLVREAFGMSEKKIHTFIYFRSFIDIPTKDGNVKYKVISVIDHEDGLENGHYYLKALHESESSSKWLLKKNSQVVEIQDSEVCKRFSIGILLEKAENGIQDVPKLLTSGKEFNSHFRSAMQAIFRIFYLCEDFKNDLISFYEKEIVNRNTLIYGTS--------------------------------NIKELCCDILQKVNFNIKKAQEIRENPKDNNYNGS----------------------------------CFPKNFKSNKRNKNTERCQNKNNLNKDNYQKGCDTPMKRECKEKFKETSEQANDVKQIKEQIYSDNEKEQPEPEIIPIIPEIDNCNGKIFELLGFKIVDFTFPEEKYFFSTEKHYLQIKKCPKTEKSQFIPKYLFLCFSVNENHAEINQGSISQAQFEERVYSLLSIVNYDQSNGRNKYYVKCVEKSAQLSGDFESISKSDIKTNTLVAALM 536

HSP 2 Score: 372.089 bits (954), Expect = 7.422e-168
Identity = 182/195 (93.33%), Postives = 186/195 (95.38%), Query Frame = 3 
Query: 2574 NKNLEGLQLAHLVRIILKSDEKTCYSSIISLLFLCPKFRDFVLEIYKKTGDVEIGKNSEMREKSPFFEICSLYHSLVSCGTLGDEYPSSEEKCTKNIFSEELGECYIALLQNLKKVFLPKFMKISDDDSVTGYSRLIHDLFGNKIVIKTSCLNCGLRTESKEFVYYLSIHLPIDKKSESIQNCVNNGFIGNVFKI 3158
            NK +  LQLAHLVRIILKSDEKTCYSSIISLLFLCPKFRDFVLEIYKKT   ++GK SEM  KSPFFEICSLYHSLVSCGTLGDEYPSSEEKCTKNIFSEELGECYIALLQNLKKVFLPKFMKISDDDSVTGYSRLIHDLFGNKIVIKTSCLNCGLRTESKEFVYYLSIHLPIDKKSESIQNCVNNGFIGNVFK+
Sbjct:  646 NKQVSRLQLAHLVRIILKSDEKTCYSSIISLLFLCPKFRDFVLEIYKKTDSAKMGKYSEMPAKSPFFEICSLYHSLVSCGTLGDEYPSSEEKCTKNIFSEELGECYIALLQNLKKVFLPKFMKISDDDSVTGYSRLIHDLFGNKIVIKTSCLNCGLRTESKEFVYYLSIHLPIDKKSESIQNCVNNGFIGNVFKV 840

HSP 3 Score: 240.35 bits (612), Expect = 7.422e-168
Identity = 118/121 (97.52%), Postives = 120/121 (99.17%), Query Frame = 2 
Query: 3152 QNNCCSKQSHSTKTKFNCCGEYLLICFDKKTRERSKSGEINMKLDDGLLLEYIKSLDNKSTQVYSLYAVIDEIQSNSGKRYEIKFLSNEKWLRVNSTEAVPVDNNAITSLENAAFLFKKVT 3514
            ++NCCSKQSHSTKTKFNCCGEYLLICFDKKTRERSKSGEINMKLDDGLLLEYIKSLDNKSTQVYSLYAVIDEIQSNSGKRYEIKFLSNEKWLRVNSTEAV VDNNAITSLENAAFLFKKVT
Sbjct:  841 KDNCCSKQSHSTKTKFNCCGEYLLICFDKKTRERSKSGEINMKLDDGLLLEYIKSLDNKSTQVYSLYAVIDEIQSNSGKRYEIKFLSNEKWLRVNSTEAVSVDNNAITSLENAAFLFKKVT 961
[Close]
BLAST of SMED30023987 vs. Planmine SMEST
Match: SMESG000038485.1 (SMESG000038485.1)

HSP 1 Score: 963.37 bits (2489), Expect = 0.000e+0
Identity = 517/599 (86.31%), Postives = 525/599 (87.65%), Query Frame = 3 
Query:  348 EDIHKCYLQILSRIDAAVTRCSTAPENIGDQNEFPVNIKTKNDESLIDLFGGRKCLVKKCVSCGKTHEKLQRFKSMPLEVPSSPWKISIEHCIFNHFRDLAIMKSELSCCEYPNIKLSSQYAVVSQYIFIYLVREAFGMSEKKIHTFIYFWSFIDIPTKDGNVKYKVISVIDHEDGLENGHYYLKALHXXXXXXKWLLKKNSQVVEIQDSEVCKRFSIGILLEKAENGIQDVPKLLTSGKEFNSHFRSAMQAIFRIFYLCEDFKNGLISFYEKEIVNRNTLIYGTRLGEILHYMSENNNKLVDILIEEFCFNWSNPFYSIKELCCDILQKVNFNIKKAKEIRENPKDNNYNDTEKSNSEKFQNKPKAEAPXXXXXXXXXXXXXXXXXXXPKNFKSNKRNKNTERCQNKNNLNKDNYQKGCDTPMKRECKEKFKETSEQANDVKQIKEQIYSDNXXXXXXXXXXXXXXXXDNCNGKIFELLGFKIVDFTFPEEKYFFSTEKHYLQIKKCPKTEKSQFIPKYLFLCFSVNENHAEINQGSISQAQFEERVYSLLSIVNYDQSNGQNKYYVKCVEKSAELSGDFESISKSDINSNTLVAALM 2144
            EDIHKCYLQILSRIDAAVTRCSTAPENIGDQNEFPVNIKTKNDESLI+LFGGRKCL+KKCVSCGKTHEK QRFKSMPLEVPSSPWKISIEHCIFNHFRD AIMKSELSCCEYPNIKLSSQY  V QYIFIYLVREAFGMSEKKIHTFIYF SFIDIPTKDGNVKYKVISVIDHEDGLENGHYYLKALHESESSSKWLLKKNSQVVEIQDSEVCKRFSIGILLEKAENGIQDVPKLLTSGKEFNSHFRSAMQAIFRIFYLCEDFKN LISFYEKEIVNRNTLIYGT                                 +IKELCCDILQKVNFNIKKA+EIRENPKDNNYN                                F+ SCFPKNFKSNKRNKNTERCQNKNNLNKDNYQKGCDTPMKRECKEKFKETSEQANDVKQIKEQIYSDNEKEQPEPEIIPIIPEIDNCNGKIFELLGFKIVDFTFPEEKYFFSTEKHYLQIKKCPKTEKSQFIPKYLFLCFSVNENHAEINQGSISQAQFEERVYSLLSIVNYDQSNG+NKYYVKCVEKSA+LSGDFESISKSDI +NTLVAALM
Sbjct:    4 EDIHKCYLQILSRIDAAVTRCSTAPENIGDQNEFPVNIKTKNDESLIELFGGRKCLLKKCVSCGKTHEKFQRFKSMPLEVPSSPWKISIEHCIFNHFRDSAIMKSELSCCEYPNIKLSSQYDAVPQYIFIYLVREAFGMSEKKIHTFIYFRSFIDIPTKDGNVKYKVISVIDHEDGLENGHYYLKALHESESSSKWLLKKNSQVVEIQDSEVCKRFSIGILLEKAENGIQDVPKLLTSGKEFNSHFRSAMQAIFRIFYLCEDFKNDLISFYEKEIVNRNTLIYGTS--------------------------------NIKELCCDILQKVNFNIKKAQEIRENPKDNNYNGN--------------------------LILHFNGSCFPKNFKSNKRNKNTERCQNKNNLNKDNYQKGCDTPMKRECKEKFKETSEQANDVKQIKEQIYSDNEKEQPEPEIIPIIPEIDNCNGKIFELLGFKIVDFTFPEEKYFFSTEKHYLQIKKCPKTEKSQFIPKYLFLCFSVNENHAEINQGSISQAQFEERVYSLLSIVNYDQSNGRNKYYVKCVEKSAQLSGDFESISKSDIKTNTLVAALM 544

HSP 2 Score: 370.933 bits (951), Expect = 1.583e-167
Identity = 182/194 (93.81%), Postives = 185/194 (95.36%), Query Frame = 3 
Query: 2574 NKNLEGLQLAHLVRIILKSDEKTCYSSIISLLFLCPKFRDFVLEIYKKTGDVEIGKNSEMREKSPFFEICSLYHSLVSCGTLGDEYPSSEEKCTKNIFSEELGECYIALLQNLKKVFLPKFMKISDDDSVTGYSRLIHDLFGNKIVIKTSCLNCGLRTESKEFVYYLSIHLPIDKKSESIQNCVNNGFIGNVFK 3155
            NK +  LQLAHLVRIILKSDEKTCYSSIISLLFLCPKFRDFVLEIYKKT   ++GK SEM  KSPFFEICSLYHSLVSCGTLGDEYPSSEEKCTKNIFSEELGECYIALLQNLKKVFLPKFMKISDDDSVTGYSRLIHDLFGNKIVIKTSCLNCGLRTESKEFVYYLSIHLPIDKKSESIQNCVNNGFIGNVFK
Sbjct:  654 NKQVSRLQLAHLVRIILKSDEKTCYSSIISLLFLCPKFRDFVLEIYKKTDSAKMGKYSEMPAKSPFFEICSLYHSLVSCGTLGDEYPSSEEKCTKNIFSEELGECYIALLQNLKKVFLPKFMKISDDDSVTGYSRLIHDLFGNKIVIKTSCLNCGLRTESKEFVYYLSIHLPIDKKSESIQNCVNNGFIGNVFK 847

HSP 3 Score: 240.35 bits (612), Expect = 1.583e-167
Identity = 118/121 (97.52%), Postives = 120/121 (99.17%), Query Frame = 2 
Query: 3152 QNNCCSKQSHSTKTKFNCCGEYLLICFDKKTRERSKSGEINMKLDDGLLLEYIKSLDNKSTQVYSLYAVIDEIQSNSGKRYEIKFLSNEKWLRVNSTEAVPVDNNAITSLENAAFLFKKVT 3514
            ++NCCSKQSHSTKTKFNCCGEYLLICFDKKTRERSKSGEINMKLDDGLLLEYIKSLDNKSTQVYSLYAVIDEIQSNSGKRYEIKFLSNEKWLRVNSTEAV VDNNAITSLENAAFLFKKVT
Sbjct:  848 KDNCCSKQSHSTKTKFNCCGEYLLICFDKKTRERSKSGEINMKLDDGLLLEYIKSLDNKSTQVYSLYAVIDEIQSNSGKRYEIKFLSNEKWLRVNSTEAVSVDNNAITSLENAAFLFKKVT 968
[Close]
BLAST of SMED30023987 vs. Planmine SMEST
Match: SMESG000038485.1 (SMESG000038485.1)

HSP 1 Score: 953.355 bits (2463), Expect = 0.000e+0
Identity = 512/599 (85.48%), Postives = 520/599 (86.81%), Query Frame = 3 
Query:  348 EDIHKCYLQILSRIDAAVTRCSTAPENIGDQNEFPVNIKTKNDESLIDLFGGRKCLVKKCVSCGKTHEKLQRFKSMPLEVPSSPWKISIEHCIFNHFRDLAIMKSELSCCEYPNIKLSSQYAVVSQYIFIYLVREAFGMSEKKIHTFIYFWSFIDIPTKDGNVKYKVISVIDHEDGLENGHYYLKALHXXXXXXKWLLKKNSQVVEIQDSEVCKRFSIGILLEKAENGIQDVPKLLTSGKEFNSHFRSAMQAIFRIFYLCEDFKNGLISFYEKEIVNRNTLIYGTRLGEILHYMSENNNKLVDILIEEFCFNWSNPFYSIKELCCDILQKVNFNIKKAKEIRENPKDNNYNDTEKSNSEKFQNKPKAEAPXXXXXXXXXXXXXXXXXXXPKNFKSNKRNKNTERCQNKNNLNKDNYQKGCDTPMKRECKEKFKETSEQANDVKQIKEQIYSDNXXXXXXXXXXXXXXXXDNCNGKIFELLGFKIVDFTFPEEKYFFSTEKHYLQIKKCPKTEKSQFIPKYLFLCFSVNENHAEINQGSISQAQFEERVYSLLSIVNYDQSNGQNKYYVKCVEKSAELSGDFESISKSDINSNTLVAALM 2144
            EDIHKCYLQILSRIDAAVTRCSTAPENIGDQNEFPVNIKTKNDESLI+LFGGRKCL+KKCVSCGKTHEK QRFKSMPLEVPSSPWKISIEHCIFNHFRD AIMKSELSCCEYPNIKLSSQY  V QYIFIYLVREAFGMSEKKIHTFIYF SFIDIPTKDGNVKYKVISVIDHEDGLENGHYYLKALHESESSSKWLLKKNSQVVEIQDSEVCKRFSIGILLEKAEN   DVPKLLTSGKEFNSHFRSAMQAIFRIFYLCEDFKN LISFYEKEIVNRNTLIYGT                                 +IKELCCDILQKVNFNIKKA+EIRENPKDNNYN +                                  CFPKNFKSNKRNKNTERCQNKNNLNKDNYQKGCDTPMKRECKEKFKETSEQANDVKQIKEQIYSDNEKEQPEPEIIPIIPEIDNCNGKIFELLGFKIVDFTFPEEKYFFSTEKHYLQIKKCPKTEKSQFIPKYLFLCFSVNENHAEINQGSISQAQFEERVYSLLSIVNYDQSNG+NKYYVKCVEKSA+LSGDFESISKSDI +NTLVAALM
Sbjct:    4 EDIHKCYLQILSRIDAAVTRCSTAPENIGDQNEFPVNIKTKNDESLIELFGGRKCLLKKCVSCGKTHEKFQRFKSMPLEVPSSPWKISIEHCIFNHFRDSAIMKSELSCCEYPNIKLSSQYDAVPQYIFIYLVREAFGMSEKKIHTFIYFRSFIDIPTKDGNVKYKVISVIDHEDGLENGHYYLKALHESESSSKWLLKKNSQVVEIQDSEVCKRFSIGILLEKAEN---DVPKLLTSGKEFNSHFRSAMQAIFRIFYLCEDFKNDLISFYEKEIVNRNTLIYGTS--------------------------------NIKELCCDILQKVNFNIKKAQEIRENPKDNNYNGS----------------------------------CFPKNFKSNKRNKNTERCQNKNNLNKDNYQKGCDTPMKRECKEKFKETSEQANDVKQIKEQIYSDNEKEQPEPEIIPIIPEIDNCNGKIFELLGFKIVDFTFPEEKYFFSTEKHYLQIKKCPKTEKSQFIPKYLFLCFSVNENHAEINQGSISQAQFEERVYSLLSIVNYDQSNGRNKYYVKCVEKSAQLSGDFESISKSDIKTNTLVAALM 533

HSP 2 Score: 372.474 bits (955), Expect = 1.655e-163
Identity = 182/195 (93.33%), Postives = 186/195 (95.38%), Query Frame = 3 
Query: 2574 NKNLEGLQLAHLVRIILKSDEKTCYSSIISLLFLCPKFRDFVLEIYKKTGDVEIGKNSEMREKSPFFEICSLYHSLVSCGTLGDEYPSSEEKCTKNIFSEELGECYIALLQNLKKVFLPKFMKISDDDSVTGYSRLIHDLFGNKIVIKTSCLNCGLRTESKEFVYYLSIHLPIDKKSESIQNCVNNGFIGNVFKI 3158
            NK +  LQLAHLVRIILKSDEKTCYSSIISLLFLCPKFRDFVLEIYKKT   ++GK SEM  KSPFFEICSLYHSLVSCGTLGDEYPSSEEKCTKNIFSEELGECYIALLQNLKKVFLPKFMKISDDDSVTGYSRLIHDLFGNKIVIKTSCLNCGLRTESKEFVYYLSIHLPIDKKSESIQNCVNNGFIGNVFK+
Sbjct:  643 NKQVSRLQLAHLVRIILKSDEKTCYSSIISLLFLCPKFRDFVLEIYKKTDSAKMGKYSEMPAKSPFFEICSLYHSLVSCGTLGDEYPSSEEKCTKNIFSEELGECYIALLQNLKKVFLPKFMKISDDDSVTGYSRLIHDLFGNKIVIKTSCLNCGLRTESKEFVYYLSIHLPIDKKSESIQNCVNNGFIGNVFKV 837

HSP 3 Score: 225.328 bits (573), Expect = 1.655e-163
Identity = 113/121 (93.39%), Postives = 115/121 (95.04%), Query Frame = 2 
Query: 3152 QNNCCSKQSHSTKTKFNCCGEYLLICFDKKTRERSKSGEINMKLDDGLLLEYIKSLDNKSTQVYSLYAVIDEIQSNSGKRYEIKFLSNEKWLRVNSTEAVPVDNNAITSLENAAFLFKKVT 3514
            ++NCCSKQSHSTKTKFNCCGEYLLICFDKKTRERSKSGEINMKLDDGLLLEY     NKSTQVYSLYAVIDEIQSNSGKRYEIKFLSNEKWLRVNSTEAV VDNNAITSLENAAFLFKKVT
Sbjct:  838 KDNCCSKQSHSTKTKFNCCGEYLLICFDKKTRERSKSGEINMKLDDGLLLEY-----NKSTQVYSLYAVIDEIQSNSGKRYEIKFLSNEKWLRVNSTEAVSVDNNAITSLENAAFLFKKVT 953
[Close]
BLAST of SMED30023987 vs. Planmine SMEST
Match: SMESG000038485.1 (SMESG000038485.1)

HSP 1 Score: 901.738 bits (2329), Expect = 0.000e+0
Identity = 492/599 (82.14%), Postives = 500/599 (83.47%), Query Frame = 3 
Query:  348 EDIHKCYLQILSRIDAAVTRCSTAPENIGDQNEFPVNIKTKNDESLIDLFGGRKCLVKKCVSCGKTHEKLQRFKSMPLEVPSSPWKISIEHCIFNHFRDLAIMKSELSCCEYPNIKLSSQYAVVSQYIFIYLVREAFGMSEKKIHTFIYFWSFIDIPTKDGNVKYKVISVIDHEDGLENGHYYLKALHXXXXXXKWLLKKNSQVVEIQDSEVCKRFSIGILLEKAENGIQDVPKLLTSGKEFNSHFRSAMQAIFRIFYLCEDFKNGLISFYEKEIVNRNTLIYGTRLGEILHYMSENNNKLVDILIEEFCFNWSNPFYSIKELCCDILQKVNFNIKKAKEIRENPKDNNYNDTEKSNSEKFQNKPKAEAPXXXXXXXXXXXXXXXXXXXPKNFKSNKRNKNTERCQNKNNLNKDNYQKGCDTPMKRECKEKFKETSEQANDVKQIKEQIYSDNXXXXXXXXXXXXXXXXDNCNGKIFELLGFKIVDFTFPEEKYFFSTEKHYLQIKKCPKTEKSQFIPKYLFLCFSVNENHAEINQGSISQAQFEERVYSLLSIVNYDQSNGQNKYYVKCVEKSAELSGDFESISKSDINSNTLVAALM 2144
            EDIHKCYLQILSRIDAAVTRCSTAPENIGDQNEFPVNIKTKNDESLI+LFGGRKCL+KKCVSCGKTHEK QRFKSMPLEVP                        ELSCCEYPNIKLSSQY  V QYIFIYLVREAFGMSEKKIHTFIYF SFIDIPTKDGNVKYKVISVIDHEDGLENGHYYLKALHESESSSKWLLKKNSQVVEIQDSEVCKRFSIGILLEKAENGIQDVPKLLTSGKEFNSHFRSAMQAIFRIFYLCEDFKN LISFYEKEIVNRNTLIYGT                                 +IKELCCDILQKVNFNIKKA+EIRENPKDNNYN +                                  CFPKNFKSNKRNKNTERCQNKNNLNKDNYQKGCDTPMKRECKEKFKETSEQANDVKQIKEQIYSDNEKEQPEPEIIPIIPEIDNCNGKIFELLGFKIVDFTFPEEKYFFSTEKHYLQIKKCPKTEKSQFIPKYLFLCFSVNENHAEINQGSISQAQFEERVYSLLSIVNYDQSNG+NKYYVKCVEKSA+LSGDFESISKSDI +NTLVAALM
Sbjct:    4 EDIHKCYLQILSRIDAAVTRCSTAPENIGDQNEFPVNIKTKNDESLIELFGGRKCLLKKCVSCGKTHEKFQRFKSMPLEVP------------------------ELSCCEYPNIKLSSQYDAVPQYIFIYLVREAFGMSEKKIHTFIYFRSFIDIPTKDGNVKYKVISVIDHEDGLENGHYYLKALHESESSSKWLLKKNSQVVEIQDSEVCKRFSIGILLEKAENGIQDVPKLLTSGKEFNSHFRSAMQAIFRIFYLCEDFKNDLISFYEKEIVNRNTLIYGTS--------------------------------NIKELCCDILQKVNFNIKKAQEIRENPKDNNYNGS----------------------------------CFPKNFKSNKRNKNTERCQNKNNLNKDNYQKGCDTPMKRECKEKFKETSEQANDVKQIKEQIYSDNEKEQPEPEIIPIIPEIDNCNGKIFELLGFKIVDFTFPEEKYFFSTEKHYLQIKKCPKTEKSQFIPKYLFLCFSVNENHAEINQGSISQAQFEERVYSLLSIVNYDQSNGRNKYYVKCVEKSAQLSGDFESISKSDIKTNTLVAALM 512

HSP 2 Score: 372.089 bits (954), Expect = 5.593e-168
Identity = 182/195 (93.33%), Postives = 186/195 (95.38%), Query Frame = 3 
Query: 2574 NKNLEGLQLAHLVRIILKSDEKTCYSSIISLLFLCPKFRDFVLEIYKKTGDVEIGKNSEMREKSPFFEICSLYHSLVSCGTLGDEYPSSEEKCTKNIFSEELGECYIALLQNLKKVFLPKFMKISDDDSVTGYSRLIHDLFGNKIVIKTSCLNCGLRTESKEFVYYLSIHLPIDKKSESIQNCVNNGFIGNVFKI 3158
            NK +  LQLAHLVRIILKSDEKTCYSSIISLLFLCPKFRDFVLEIYKKT   ++GK SEM  KSPFFEICSLYHSLVSCGTLGDEYPSSEEKCTKNIFSEELGECYIALLQNLKKVFLPKFMKISDDDSVTGYSRLIHDLFGNKIVIKTSCLNCGLRTESKEFVYYLSIHLPIDKKSESIQNCVNNGFIGNVFK+
Sbjct:  622 NKQVSRLQLAHLVRIILKSDEKTCYSSIISLLFLCPKFRDFVLEIYKKTDSAKMGKYSEMPAKSPFFEICSLYHSLVSCGTLGDEYPSSEEKCTKNIFSEELGECYIALLQNLKKVFLPKFMKISDDDSVTGYSRLIHDLFGNKIVIKTSCLNCGLRTESKEFVYYLSIHLPIDKKSESIQNCVNNGFIGNVFKV 816

HSP 3 Score: 240.736 bits (613), Expect = 5.593e-168
Identity = 118/121 (97.52%), Postives = 120/121 (99.17%), Query Frame = 2 
Query: 3152 QNNCCSKQSHSTKTKFNCCGEYLLICFDKKTRERSKSGEINMKLDDGLLLEYIKSLDNKSTQVYSLYAVIDEIQSNSGKRYEIKFLSNEKWLRVNSTEAVPVDNNAITSLENAAFLFKKVT 3514
            ++NCCSKQSHSTKTKFNCCGEYLLICFDKKTRERSKSGEINMKLDDGLLLEYIKSLDNKSTQVYSLYAVIDEIQSNSGKRYEIKFLSNEKWLRVNSTEAV VDNNAITSLENAAFLFKKVT
Sbjct:  817 KDNCCSKQSHSTKTKFNCCGEYLLICFDKKTRERSKSGEINMKLDDGLLLEYIKSLDNKSTQVYSLYAVIDEIQSNSGKRYEIKFLSNEKWLRVNSTEAVSVDNNAITSLENAAFLFKKVT 937
The following BLAST results are available for this feature:
BLAST of SMED30023987 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023987 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30023987 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30023987 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023987 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023987 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023987 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023987 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A553MUV91.945e-722.64USP domain-containing protein (Fragment) OS=Danion... [more]
A0A553MUW02.734e-722.64USP domain-containing protein (Fragment) OS=Danion... [more]
A0CTQ87.974e-725.27Uncharacterized protein OS=Paramecium tetraurelia ... [more]
A0CEF39.701e-624.91Uncharacterized protein OS=Paramecium tetraurelia ... [more]
A0EB129.901e-621.70Uncharacterized protein OS=Paramecium tetraurelia ... [more]
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BLAST of SMED30023987 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023987 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023987 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023987 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023987 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023987 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000038485.14.671e-16786.64SMESG000038485.1[more]
SMESG000038485.17.422e-16885.98SMESG000038485.1[more]
SMESG000038485.11.583e-16786.31SMESG000038485.1[more]
SMESG000038485.11.655e-16385.48SMESG000038485.1[more]
SMESG000038485.15.593e-16882.14SMESG000038485.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30023987 ID=SMED30023987|Name=SMED30023987|organism=Schmidtea mediterranea sexual|type=transcript|length=3660bp
CTATATAAATCTTATATGATACATTTCATTTAATTTTCAAGATTAAAATA
TTTGCATTTCAATTAGAGTTACATTTTGTTATGGAGTGTAATCAATCCCA
TAATATAGGTTTCCCAATGCCACAATCATATTGTTATTTAAATTCAGTTT
TACAAATGTTTTTTTCTTGTAAAATATTCCGACTAAATCTTAGCGAGTGG
ATAAAAAGCAATCAGAATATCAATGAAAATACAAATTTTCTTTATGCGTT
GGAGGATTTATTCAAGTCCATGGAATCTCGGAGAGAGAATAATGCTCAAT
TTGCAATTCATGAATTGCAAAACGTCTTTCAAACTTTATCATCGGAAGAG
GATATTCACAAGTGCTACCTTCAGATATTGAGCAGGATTGATGCCGCCGT
TACTAGATGCTCGACCGCACCAGAAAATATTGGTGACCAAAATGAATTTC
CAGTAAATATTAAAACAAAAAATGATGAATCTTTGATTGACTTATTTGGA
GGTCGTAAATGTTTAGTAAAAAAATGTGTTTCATGTGGCAAAACTCATGA
AAAACTTCAAAGATTCAAATCAATGCCATTGGAAGTTCCGTCGTCTCCCT
GGAAAATTTCAATTGAGCATTGCATTTTCAATCATTTTAGAGATTTAGCA
ATTATGAAATCGGAATTGTCTTGCTGTGAATATCCAAATATCAAGCTTTC
CTCACAATATGCTGTGGTCTCACAATACATTTTTATCTATTTAGTTCGCG
AGGCTTTCGGAATGTCTGAAAAGAAAATTCATACTTTCATATATTTTTGG
AGTTTTATAGATATCCCAACGAAGGATGGAAATGTAAAATATAAAGTCAT
ATCGGTAATTGACCATGAAGATGGATTGGAAAATGGCCACTATTATTTGA
AAGCTCTCCATGAAAGTGAAAGCTCTTCTAAATGGCTGTTAAAAAAGAAT
TCACAAGTTGTCGAAATCCAAGATTCTGAAGTTTGCAAAAGATTTTCCAT
TGGAATATTGCTTGAAAAAGCCGAAAATGGTATTCAAGATGTTCCTAAAT
TGTTGACTTCTGGAAAAGAATTCAATTCTCATTTCCGGAGCGCCATGCAA
GCAATTTTTCGAATTTTTTACTTATGTGAAGATTTTAAAAATGGTCTTAT
CAGTTTCTATGAAAAAGAAATAGTTAATAGAAATACTTTAATTTATGGTA
CAAGACTCGGCGAGATCTTGCATTATATGTCAGAAAATAATAATAAATTG
GTTGACATTTTAATAGAGGAATTTTGTTTTAATTGGTCAAATCCTTTTTA
TAGTATCAAAGAATTATGTTGCGATATTTTACAAAAAGTTAATTTTAACA
TAAAGAAAGCTAAAGAGATCCGTGAAAATCCAAAGGATAATAATTATAAT
GATACGGAAAAATCAAATTCAGAAAAGTTTCAAAATAAACCGAAAGCCGA
AGCTCCTACCTCAACAAAATTAAAAACATCAAAAAAATCCCGTTCATTTT
CATGTTCATGTTTCCCTAAGAATTTCAAAAGTAATAAACGAAATAAAAAC
ACAGAAAGATGTCAAAATAAAAATAATTTGAATAAAGATAATTATCAGAA
AGGCTGTGATACACCAATGAAAAGAGAATGTAAAGAAAAATTTAAGGAAA
CATCAGAACAAGCAAATGATGTCAAGCAAATAAAAGAACAAATATATTCC
GATAATGAAAAGGAGCAGCCGGAACCTGAAATTATCCCAATAATTCCAGA
AATAGATAATTGCAATGGAAAAATATTCGAACTTTTGGGATTCAAAATCG
TTGATTTTACTTTTCCAGAAGAAAAATATTTTTTTTCAACGGAAAAACAT
TATTTGCAAATTAAGAAATGCCCGAAGACTGAAAAAAGCCAATTTATACC
GAAATATTTGTTTCTTTGTTTTAGTGTGAATGAAAATCATGCTGAAATTA
ACCAAGGAAGTATCAGTCAAGCTCAATTTGAAGAACGGGTTTATTCATTG
CTTTCTATTGTAAATTATGATCAATCAAATGGGCAAAATAAATATTATGT
AAAATGTGTTGAAAAATCGGCAGAATTATCTGGAGATTTTGAATCTATTT
CTAAATCTGATATTAATTCGAATACTCTCGTAGCTGCTCTGATGGAACTG
AAAGAACCGCCAAAAAATGAAGAAAAAAATGAGAAGAAAATCGAGGAGCA
AAATAATTACCTAAATAAAGAGAAAAATAATGACAGAAATGAGGAGCGAA
ATGAGGATAAAAATGAGAAAATAGAGGATAAATATGATAACCAAAACGAA
GTGAAAAGAGAGGAGAAAGTTGTAGGTGAAAATAATAAGCAAAATTATGA
TCAAAATAAAGACAAAACTGAGGGGGGGAAACAGAAGAAAAAGGAAGAGA
AAAATAATGATAAAAATGATCAGCGAAATGAGGAGAAATATGATAATCAA
AACGAAGGGAAAAGAGAGGAGAAAAATGTAGGTGAAAATGAGAAGCAAAA
TGATGACCAAAATGAAGAGAAAAATAATGAAAGAAATGAGGAAAAAGTGA
ATTTATTTGAAATGAATCTTTCGAACAAAAATCTCGAAGGACTTCAATTA
GCTCATTTAGTTCGAATCATTTTGAAATCCGACGAAAAAACTTGTTATTC
TTCTATAATAAGTTTATTATTTCTCTGCCCTAAATTTAGAGATTTTGTGC
TGGAAATATATAAAAAGACTGGCGACGTAGAGATAGGTAAAAATAGTGAA
ATGCGGGAGAAATCTCCATTTTTTGAAATCTGCTCCTTATATCATAGCTT
AGTCTCCTGTGGGACCCTTGGTGATGAATATCCTTCCTCAGAGGAAAAAT
GCACCAAAAATATATTTTCCGAAGAACTCGGTGAATGTTACATAGCTTTG
TTGCAAAATCTTAAAAAAGTTTTTTTGCCTAAATTTATGAAAATTTCTGA
TGATGACTCCGTTACCGGTTACAGTCGCTTGATTCATGATTTATTTGGAA
ACAAAATTGTTATCAAAACTTCCTGTTTAAATTGTGGACTGAGAACAGAA
TCCAAAGAATTTGTTTATTATCTTTCAATTCACTTACCTATTGATAAAAA
AAGTGAAAGTATTCAGAATTGTGTGAATAATGGCTTCATAGGAAATGTTT
TCAAAATAATTGCTGTTCCAAGCAGTCTCATTCAACTAAAACTAAATTCA
ATTGTTGCGGAGAATATCTGTTAATATGTTTTGATAAAAAGACACGTGAG
AGAAGTAAAAGTGGAGAAATCAATATGAAACTCGATGATGGACTTTTATT
AGAATATATTAAAAGCTTAGATAATAAAAGCACACAAGTTTATTCATTAT
ACGCAGTGATTGATGAAATCCAATCAAATTCTGGAAAGCGGTATGAAATT
AAATTTCTATCCAATGAAAAATGGTTGCGAGTTAATTCTACTGAAGCTGT
CCCTGTGGATAATAATGCCATTACGTCACTTGAAAATGCGGCTTTTTTAT
TCAAAAAAGTTACATGAAATGTATTCAAAGTTTTATGATTTTGACTTTAT
AAATTTTATTGAGATCGTTTATTTGATGCTGAATTTTACATTTTTAATTT
TATTTAATGTATTTCATGATAAGTTTATTATTTTCTTACTTTATTAAACG
TCATTTTTTG
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protein sequence of SMED30023987-orf-1

>SMED30023987-orf-1 ID=SMED30023987-orf-1|Name=SMED30023987-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=1048bp
MECNQSHNIGFPMPQSYCYLNSVLQMFFSCKIFRLNLSEWIKSNQNINEN
TNFLYALEDLFKSMESRRENNAQFAIHELQNVFQTLSSEEDIHKCYLQIL
SRIDAAVTRCSTAPENIGDQNEFPVNIKTKNDESLIDLFGGRKCLVKKCV
SCGKTHEKLQRFKSMPLEVPSSPWKISIEHCIFNHFRDLAIMKSELSCCE
YPNIKLSSQYAVVSQYIFIYLVREAFGMSEKKIHTFIYFWSFIDIPTKDG
NVKYKVISVIDHEDGLENGHYYLKALHESESSSKWLLKKNSQVVEIQDSE
VCKRFSIGILLEKAENGIQDVPKLLTSGKEFNSHFRSAMQAIFRIFYLCE
DFKNGLISFYEKEIVNRNTLIYGTRLGEILHYMSENNNKLVDILIEEFCF
NWSNPFYSIKELCCDILQKVNFNIKKAKEIRENPKDNNYNDTEKSNSEKF
QNKPKAEAPTSTKLKTSKKSRSFSCSCFPKNFKSNKRNKNTERCQNKNNL
NKDNYQKGCDTPMKRECKEKFKETSEQANDVKQIKEQIYSDNEKEQPEPE
IIPIIPEIDNCNGKIFELLGFKIVDFTFPEEKYFFSTEKHYLQIKKCPKT
EKSQFIPKYLFLCFSVNENHAEINQGSISQAQFEERVYSLLSIVNYDQSN
GQNKYYVKCVEKSAELSGDFESISKSDINSNTLVAALMELKEPPKNEEKN
EKKIEEQNNYLNKEKNNDRNEERNEDKNEKIEDKYDNQNEVKREEKVVGE
NNKQNYDQNKDKTEGGKQKKKEEKNNDKNDQRNEEKYDNQNEGKREEKNV
GENEKQNDDQNEEKNNERNEEKVNLFEMNLSNKNLEGLQLAHLVRIILKS
DEKTCYSSIISLLFLCPKFRDFVLEIYKKTGDVEIGKNSEMREKSPFFEI
CSLYHSLVSCGTLGDEYPSSEEKCTKNIFSEELGECYIALLQNLKKVFLP
KFMKISDDDSVTGYSRLIHDLFGNKIVIKTSCLNCGLRTESKEFVYYLSI
HLPIDKKSESIQNCVNNGFIGNVFKIIAVPSSLIQLKLNSIVAENIC*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0002089reproductive organ
Vocabulary: INTERPRO
TermDefinition
IPR001394Peptidase_C19_UCH
IPR028889USP_dom
Vocabulary: biological process
TermDefinition
GO:0016579protein deubiquitination
GO:0006511ubiquitin-dependent protein catabolic process
Vocabulary: molecular function
TermDefinition
GO:0036459thiol-dependent ubiquitinyl hydrolase activity