SMED30023981

Overview


Name	SMED30023981
Smed ID	SMED30023981
Length (bp)	2404

Neoblast Clusters

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.

Expression of SMED30023981 (SMED30023981) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30023981 (SMED30023981) in cells (dots) of each of the 12 neoblast clusters.

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30023981 (SMED30023981) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30023981 (SMED30023981) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Alignments

SMED30023981 aligns in the following genomic locations:


Alignment Location	Alignment Score
v31.000959:27190..30782 +	11345

Homology

[Close]

BLAST of SMED30023981 vs. Ensembl Human
Match: POLRMT (RNA polymerase mitochondrial [Source:HGNC Symbol;Acc:HGNC:9200])

HSP 1 Score: 157.532 bits (397), Expect = 1.786e-51
Identity = 79/167 (47.31%), Postives = 105/167 (62.87%), Query Frame = -2

Query: 1561 ISWKTPMNFIVKQPYFAD-----------------DSDI-LKSDCLKNKNAFPPNFIHSLDSTHMMLTALSCWEKNITFVSVHDCFWTHACDVDEMNKFCREEFVKLHSQPILENLSDYLLEKYQFQINPNENDNGRKIAFNKLLKNIPKKGTFDIREVLDSTYFFS 2007
            + W TP+   V QPY  D                 + DI  K +  K KN FPPNFIHSLDS+HMMLTAL C+ K +TFVSVHDC+WTHA DV  MN+ CRE+FV+LHS+PIL++LS +L+++  F   P +     ++   + L+ +PK G FD+ +V  STYFFS
Sbjct: 1068 VEWVTPLGVPVIQPYRLDSKVKQIGGGIQSITYTHNGDISRKPNTRKQKNGFPPNFIHSLDSSHMMLTALHCYRKGLTFVSVHDCYWTHAADVSVMNQVCREQFVRLHSEPILQDLSRFLVKR--FCSEPQKILEASQL--KETLQAVPKPGAFDLEQVKRSTYFFS 1230

HSP 2 Score: 67.0106 bits (162), Expect = 1.786e-51
Identity = 48/104 (46.15%), Postives = 68/104 (65.38%), Query Frame = -3

Query: 2091 QDVYSDVLEHLEVKRKEDAKNGVEEAILLEGLLSRKVIKXXXXXXXXXXXXXXXTLQILRELKNLEKLPPQNIKSCAAYLSKLTLGSIGEIFASATNIQNWFIE 2402
            QDVYS V   +EV R++DA+ G+  A +LEG ++RKV+KQTVMT VYGVT+YGG LQI + L+ L   P + +   + YL +    S+ E+F+    IQ+W  E
Sbjct:  953 QDVYSGVAAQVEVFRRQDAQRGMRVAQVLEGFITRKVVKQTVMTVVYGVTRYGGRLQIEKRLRELSDFPQEFVWEASHYLVRQVFKSLQEMFSGTRAIQHWLTE 1056

[Close]

BLAST of SMED30023981 vs. Ensembl Human
Match: POLRMT (RNA polymerase mitochondrial [Source:HGNC Symbol;Acc:HGNC:9200])

HSP 1 Score: 125.176 bits (313), Expect = 5.179e-33
Identity = 53/78 (67.95%), Postives = 68/78 (87.18%), Query Frame = -2

Query: 1690 KNKNAFPPNFIHSLDSTHMMLTALSCWEKNITFVSVHDCFWTHACDVDEMNKFCREEFVKLHSQPILENLSDYLLEKY 1923
            K KN FPPNFIHSLDS+HMMLTAL C+ K +TFVSVHDC+WTHA DV  MN+ CRE+FV+LHS+PIL++LS +L++++
Sbjct:   31 KQKNGFPPNFIHSLDSSHMMLTALHCYRKGLTFVSVHDCYWTHAADVSVMNQVCREQFVRLHSEPILQDLSRFLVKRF 108

[Close]

BLAST of SMED30023981 vs. Ensembl Human
Match: KANSL3 (KAT8 regulatory NSL complex subunit 3 [Source:HGNC Symbol;Acc:HGNC:25473])

HSP 1 Score: 67.781 bits (164), Expect = 7.043e-11
Identity = 69/327 (21.10%), Postives = 140/327 (42.81%), Query Frame = 2

Query:  518 QISKRIYYTYAASHWRMDICSTAHAYLLENLSDEYLVIYIESLRVMYHRKKVRTILDQLNI---------WAQNQKILHRFSWASLLEII-HREPEKL-----------NEDLSEFSWNIENR-WK----------PIIIHIYSDRWSDSNRLQKFIKILKTFSTVVDFPFDDSGRLRKKFEALRNQLQLELKHKKSSVFVMGWDLGALLGLLSSIKIHGYFTSVFCGVICLGLPLKGLKGERGIIPEDYIHLLTKETLIISGDMSSLGNGDDIEDFKDRMKGRVDIKHINIGGCDHLLRLSSGYAMRYRISQDVVDRLIAKNIRNF 1402
            + ++R+    A+  W   +    H  L+E LS   L  Y+++L+ +  + K+ T++D++ +          A+   +L +  W   + ++ H +P KL               S F  +  +R W+          P+  H+ ++           + +L+    ++       G +R K       L++        + ++GW+ GAL+     + +  Y T+V    +CLG PL  + G RG + +D +  +    L + G  S   + + +EDF++++  R +   + +GG D  LR+S        ++Q +VDR I   I +F
Sbjct:  173 KCARRVRQALASVSWDTKLIQWLHTTLVETLSLPMLAAYLDALQTL--KGKIPTLIDRMLVSSNTKTGAAGAEALSLLLKRPWDPAVGVLSHNKPSKLPGSPLILIASSGPSSSVFPTSRRHRFWQSQLSCLGKVIPVATHLLNN--------GSGVGVLQCLEHMI-------GAVRSKV------LEIHSHFPHKPIILIGWNTGALVAC--HVSVMEYVTAV----VCLGFPLLTVDGPRGDV-DDPLLDMKTPVLFVIGQNSLQCHPEAMEDFREKI--RAENSLVVVGGADDNLRISKAKKKSEGLTQSMVDRCIQDEIVDF 467

[Close]

BLAST of SMED30023981 vs. Ensembl Human
Match: KANSL3 (KAT8 regulatory NSL complex subunit 3 [Source:HGNC Symbol;Acc:HGNC:25473])

HSP 1 Score: 67.781 bits (164), Expect = 7.557e-11
Identity = 70/327 (21.41%), Postives = 141/327 (43.12%), Query Frame = 2

Query:  518 QISKRIYYTYAASHWRMDICSTAHAYLLENLSDEYLVIYIESLRVMYHRKKVRTILDQLNI---------WAQNQKILHRFSWASLLEII-HREPEKL-----------NEDLSEFSWNIENR-WK----------PIIIHIYSDRWSDSNRLQKFIKILKTFSTVVDFPFDDSGRLRKKFEALRNQLQLELKHKKSSVFVMGWDLGALLGLLSSIKIHGYFTSVFCGVICLGLPLKGLKGERGIIPEDYIHLLTKETLIISGDMSSLGNGDDIEDFKDRMKGRVDIKHINIGGCDHLLRLSSGYAMRYRISQDVVDRLIAKNIRNF 1402
            + ++R+    A+  W   +    H  L+E LS   L  Y+++L+ +  + K+ T++D++ +          A+   +L +  W   + ++ H +P KL               S F  +  +R W+          P+  H+ ++           + +L+    ++       G +R K      ++     HK   + ++GW+ GAL+     + +  Y T+V    +CLG PL  + G RG + +D +  +    L + G  S   + + +EDF++++  R +   + +GG D  LR+S        ++Q +VDR I   I +F
Sbjct:  173 KCARRVRQALASVSWDTKLIQWLHTTLVETLSLPMLAAYLDALQTL--KGKIPTLIDRMLVSSNTKTGAAGAEALSLLLKRPWDPAVGVLSHNKPSKLPGSPLILIASSGPSSSVFPTSRRHRFWQSQLSCLGKVIPVATHLLNN--------GSGVGVLQCLEHMI-------GAVRSKVL----EIHSHFPHKP--IILIGWNTGALVAC--HVSVMEYVTAV----VCLGFPLLTVDGPRGDV-DDPLLDMKTPVLFVIGQNSLQCHPEAMEDFREKI--RAENSLVVVGGADDNLRISKAKKKSEGLTQSMVDRCIQDEIVDF 467

[Close]

BLAST of SMED30023981 vs. Ensembl Human
Match: KANSL3 (KAT8 regulatory NSL complex subunit 3 [Source:HGNC Symbol;Acc:HGNC:25473])

HSP 1 Score: 67.3958 bits (163), Expect = 8.521e-11
Identity = 70/327 (21.41%), Postives = 141/327 (43.12%), Query Frame = 2

Query:  518 QISKRIYYTYAASHWRMDICSTAHAYLLENLSDEYLVIYIESLRVMYHRKKVRTILDQLNI---------WAQNQKILHRFSWASLLEII-HREPEKL-----------NEDLSEFSWNIENR-WK----------PIIIHIYSDRWSDSNRLQKFIKILKTFSTVVDFPFDDSGRLRKKFEALRNQLQLELKHKKSSVFVMGWDLGALLGLLSSIKIHGYFTSVFCGVICLGLPLKGLKGERGIIPEDYIHLLTKETLIISGDMSSLGNGDDIEDFKDRMKGRVDIKHINIGGCDHLLRLSSGYAMRYRISQDVVDRLIAKNIRNF 1402
            + ++R+    A+  W   +    H  L+E LS   L  Y+++L+ +  + K+ T++D++ +          A+   +L +  W   + ++ H +P KL               S F  +  +R W+          P+  H+ ++           + +L+    ++       G +R K      ++     HK   + ++GW+ GAL+     + +  Y T+V    +CLG PL  + G RG + +D +  +    L + G  S   + + +EDF++++  R +   + +GG D  LR+S        ++Q +VDR I   I +F
Sbjct:  173 KCARRVRQALASVSWDTKLIQWLHTTLVETLSLPMLAAYLDALQTL--KGKIPTLIDRMLVSSNTKTGAAGAEALSLLLKRPWDPAVGVLSHNKPSKLPGSPLILIASSGPSSSVFPTSRRHRFWQSQLSCLGKVIPVATHLLNN--------GSGVGVLQCLEHMI-------GAVRSKVL----EIHSHFPHKP--IILIGWNTGALVAC--HVSVMEYVTAV----VCLGFPLLTVDGPRGDV-DDPLLDMKTPVLFVIGQNSLQCHPEAMEDFREKI--RAENSLVVVGGADDNLRISKAKKKSEGLTQSMVDRCIQDEIVDF 467

[Close]

BLAST of SMED30023981 vs. Ensembl Celegans
Match: rpom-1 (DNA-directed RNA polymerase [Source:UniProtKB/TrEMBL;Acc:Q8WQA3])

HSP 1 Score: 136.346 bits (342), Expect = 3.348e-42
Identity = 70/157 (44.59%), Postives = 97/157 (61.78%), Query Frame = -2

Query: 1561 ISWKTPMNFIVKQPYFA----DDSDILKSDCLKNKNAFPPNFIHSLDSTHMMLTALSCWEKNITFVSVHDCFWTHACDVDEMNKFCREEFVKLHSQPILENLSDYLLEKY----QFQINPNENDNGRKIAFNKLLKNIPKKGTFDIREVLDSTYFFS 2007
            + W TP+   V QPY          IL    +K  +AFPPNF+HSLDSTHMMLT+L+C ++ ITF +VHDCFWTHA  VD+MN+ CR++FV LHSQPI+E  S++  + Y      +I P ++       ++++     + G  DI +V DS YFFS
Sbjct:  986 VEWITPLGLPVVQPYCKLVERKGKLILAPVPMKQVDAFPPNFVHSLDSTHMMLTSLNCAQRGITFAAVHDCFWTHANSVDQMNEICRQQFVSLHSQPIVEQCSEWFKKTYLTPKMAKILPEKDFQ----KYSEIFTANIEHGDLDIEKVKDSVYFFS 1138

HSP 2 Score: 56.225 bits (134), Expect = 3.348e-42
Identity = 51/138 (36.96%), Postives = 82/138 (59.42%), Query Frame = -3

Query: 2007 DVYSDVLEHLEVKRKEDAKNGVEEAILLEGL-------LSRKVIKXXXXXXXXXXXXXXXTLQILRELKNLEKLPPQNIKSCAAYLSKLTLGSIGEIFASATNIQNWFIEVLIVAKY*KIFIKFASCLLTSVDYAKPI 2399
            DVYSDV + +E KR++D ++  E+  +   L       + RKVIKQTVMTTVYGVT YG  LQI R+LK ++ +P ++    A YL++ T  S+ + F S+  +++WF          ++  K +S L+ +V++  P+
Sbjct:  866 DVYSDVAQRVEQKRQQDEQSNGEDCDVARKLREALPQNVPRKVIKQTVMTTVYGVTMYGAVLQIKRQLKAMD-IPGEDAAIFARYLARKTFASLNDAFTSSMALKDWF----------RLIAKGSSDLMKTVEWITPL 992

[Close]

Query: 1561 ISWKTPMNFIVKQPYFA----DDSDILKSDCLKNKNAFPPNFIHSLDSTHMMLTALSCWEKNITFVSVHDCFWTHACDVDEMNKFCREEFVKLHSQPILENLSDYLLEKY----QFQINPNENDNGRKIAFNKLLKNIPKKGTFDIREVLDSTYFFS 2007
            + W TP+   V QPY          IL    +K  +AFPPNF+HSLDSTHMMLT+L+C ++ ITF +VHDCFWTHA  VD+MN+ CR++FV LHSQPI+E  S++  + Y      +I P ++       ++++     + G  DI +V DS YFFS
Sbjct:  986 VEWITPLGLPVVQPYCKLVERKGKLILAPVPMKQVDAFPPNFVHSLDSTHMMLTSLNCAQRGITFAAVHDCFWTHANSVDQMNEICRQQFVSLHSQPIVEQCSEWFKKTYLTPKMAKILPEKDFQ----KYSEIFTANIEHGDLDIEKVKDSVYFFS 1138

HSP 2 Score: 56.225 bits (134), Expect = 3.348e-42
Identity = 51/138 (36.96%), Postives = 82/138 (59.42%), Query Frame = -3

Query: 2007 DVYSDVLEHLEVKRKEDAKNGVEEAILLEGL-------LSRKVIKXXXXXXXXXXXXXXXTLQILRELKNLEKLPPQNIKSCAAYLSKLTLGSIGEIFASATNIQNWFIEVLIVAKY*KIFIKFASCLLTSVDYAKPI 2399
            DVYSDV + +E KR++D ++  E+  +   L       + RKVIKQTVMTTVYGVT YG  LQI R+LK ++ +P ++    A YL++ T  S+ + F S+  +++WF          ++  K +S L+ +V++  P+
Sbjct:  866 DVYSDVAQRVEQKRQQDEQSNGEDCDVARKLREALPQNVPRKVIKQTVMTTVYGVTMYGAVLQIKRQLKAMD-IPGEDAAIFARYLARKTFASLNDAFTSSMALKDWF----------RLIAKGSSDLMKTVEWITPL 992

[Close]

BLAST of SMED30023981 vs. Ensembl Celegans
Match: rpom-1 (DNA-directed RNA polymerase [Source:UniProtKB/TrEMBL;Acc:Q8WQA3])

HSP 1 Score: 136.346 bits (342), Expect = 1.064e-39
Identity = 70/157 (44.59%), Postives = 97/157 (61.78%), Query Frame = -2

Query: 1561 ISWKTPMNFIVKQPYFA----DDSDILKSDCLKNKNAFPPNFIHSLDSTHMMLTALSCWEKNITFVSVHDCFWTHACDVDEMNKFCREEFVKLHSQPILENLSDYLLEKY----QFQINPNENDNGRKIAFNKLLKNIPKKGTFDIREVLDSTYFFS 2007
            + W TP+   V QPY          IL    +K  +AFPPNF+HSLDSTHMMLT+L+C ++ ITF +VHDCFWTHA  VD+MN+ CR++FV LHSQPI+E  S++  + Y      +I P ++       ++++     + G  DI +V DS YFFS
Sbjct: 1004 VEWITPLGLPVVQPYCKLVERKGKLILAPVPMKQVDAFPPNFVHSLDSTHMMLTSLNCAQRGITFAAVHDCFWTHANSVDQMNEICRQQFVSLHSQPIVEQCSEWFKKTYLTPKMAKILPEKDFQ----KYSEIFTANIEHGDLDIEKVKDSVYFFS 1156

HSP 2 Score: 47.3654 bits (111), Expect = 1.064e-39
Identity = 51/146 (34.93%), Postives = 85/146 (58.22%), Query Frame = -3

Query: 2007 DVYSDVLEHLEVKRKEDAKNGVEEAILLEGL-------LSRKVIKXXXXXXXXXXXXXXXTLQILRELKNLEKLPPQNIKSCAAYLSKLTLGSIGE--IFA------SATNIQNWFIEVLIVAKY*KIFIKFASCLLTSVDYAKPI 2399
            DVYSDV + +E KR++D ++  E+  +   L       + RKVIKQTVMTTVYGVT YG  LQI R+LK ++ +P ++    A YL++ T  S+ +  IFA      +  ++ + F   + +  + ++  K +S L+ +V++  P+
Sbjct:  866 DVYSDVAQRVEQKRQQDEQSNGEDCDVARKLREALPQNVPRKVIKQTVMTTVYGVTMYGAVLQIKRQLKAMD-IPGEDAAIFARYLARKTFASLNDAAIFARYLARKTFASLNDAFTSSMALKDWFRLIAKGSSDLMKTVEWITPL 1010

[Close]

BLAST of SMED30023981 vs. Ensembl Fly
Match: mtRNApol (gene:FBgn0261938 transcript:FBtr0332273)

HSP 1 Score: 172.555 bits (436), Expect = 1.631e-57
Identity = 88/178 (49.44%), Postives = 113/178 (63.48%), Query Frame = -2

Query: 1561 ISRLRKANISWKTPMNFIVKQPY------------FADDSDI-----LKSDCLKNKNAFPPNFIHSLDSTHMMLTALSCWEKNITFVSVHDCFWTHACDVDEMNKFCREEFVKLHSQPILENLSDYLLEKYQFQ----INPNENDNGRKIAFNKLLKNIPKKGTFDIREVLDSTYFFS 2031
            IS +   N+ W TP+   V QPY            F   +++      + + LK KNAFPPNFIHSLDS+HMMLT+L C  + ITFVSVHDCFWTHA  V E+N+ CRE+FV LHSQPILE LS+++   Y F+    IN    ++  K   N++LK +P+KG FD+R VLDS YFFS
Sbjct: 1192 ISGVCSQNVEWVTPLGLPVVQPYNRQEMKHSPRSGFKVSANMPMDLYERPNILKQKNAFPPNFIHSLDSSHMMLTSLHCERQGITFVSVHDCFWTHANTVPELNRMCREQFVALHSQPILEQLSEFMRHTYSFKDSDFINDGSVEDLSKRQLNRILKQMPQKGDFDLRNVLDSVYFFS 1369

HSP 2 Score: 71.2478 bits (173), Expect = 1.631e-57
Identity = 53/104 (50.96%), Postives = 68/104 (65.38%), Query Frame = -3

Query: 2091 QDVYSDVLEHLEVKRKEDAKNGVEEAILLEGLLSRKVIKXXXXXXXXXXXXXXXTLQILRELKNLEKLPPQNIKSCAAYLSKLTLGSIGEIFASATNIQNWFIE 2402
            QDVYS V   +E  RK DA+NG+  A  L G + RKVIKQTVMTTVYGVT+YG  LQI R+LK+++  P   +   + YL+  T  S+ E+F S   IQ+WF E
Sbjct: 1084 QDVYSAVAALVEKSRKADAQNGLHVAEALAGFVRRKVIKQTVMTTVYGVTRYGARLQIARQLKDIDDFPKDWVWPASTYLTTKTFESLREMFTSTREIQDWFTE 1187

[Close]

BLAST of SMED30023981 vs. Ensembl Fly
Match: mtRNApol (gene:FBgn0261938 transcript:FBtr0077989)

HSP 1 Score: 172.555 bits (436), Expect = 1.631e-57
Identity = 88/178 (49.44%), Postives = 113/178 (63.48%), Query Frame = -2

Query: 1561 ISRLRKANISWKTPMNFIVKQPY------------FADDSDI-----LKSDCLKNKNAFPPNFIHSLDSTHMMLTALSCWEKNITFVSVHDCFWTHACDVDEMNKFCREEFVKLHSQPILENLSDYLLEKYQFQ----INPNENDNGRKIAFNKLLKNIPKKGTFDIREVLDSTYFFS 2031
            IS +   N+ W TP+   V QPY            F   +++      + + LK KNAFPPNFIHSLDS+HMMLT+L C  + ITFVSVHDCFWTHA  V E+N+ CRE+FV LHSQPILE LS+++   Y F+    IN    ++  K   N++LK +P+KG FD+R VLDS YFFS
Sbjct: 1192 ISGVCSQNVEWVTPLGLPVVQPYNRQEMKHSPRSGFKVSANMPMDLYERPNILKQKNAFPPNFIHSLDSSHMMLTSLHCERQGITFVSVHDCFWTHANTVPELNRMCREQFVALHSQPILEQLSEFMRHTYSFKDSDFINDGSVEDLSKRQLNRILKQMPQKGDFDLRNVLDSVYFFS 1369

HSP 2 Score: 71.2478 bits (173), Expect = 1.631e-57
Identity = 53/104 (50.96%), Postives = 68/104 (65.38%), Query Frame = -3

Query: 2091 QDVYSDVLEHLEVKRKEDAKNGVEEAILLEGLLSRKVIKXXXXXXXXXXXXXXXTLQILRELKNLEKLPPQNIKSCAAYLSKLTLGSIGEIFASATNIQNWFIE 2402
            QDVYS V   +E  RK DA+NG+  A  L G + RKVIKQTVMTTVYGVT+YG  LQI R+LK+++  P   +   + YL+  T  S+ E+F S   IQ+WF E
Sbjct: 1084 QDVYSAVAALVEKSRKADAQNGLHVAEALAGFVRRKVIKQTVMTTVYGVTRYGARLQIARQLKDIDDFPKDWVWPASTYLTTKTFESLREMFTSTREIQDWFTE 1187

[Close]

BLAST of SMED30023981 vs. Ensembl Zebrafish
Match: polrmt (polymerase (RNA) mitochondrial (DNA directed) [Source:ZFIN;Acc:ZDB-GENE-030131-1528])

HSP 1 Score: 162.925 bits (411), Expect = 3.988e-51
Identity = 83/171 (48.54%), Postives = 104/171 (60.82%), Query Frame = -2

Query: 1561 NISWKTPMNFIVKQPYFADDSDILKS-----------------DCLKNKNAFPPNFIHSLDSTHMMLTALSCWEKNITFVSVHDCFWTHACDVDEMNKFCREEFVKLHSQPILENLSDYLLEKYQFQINPNENDNGRKI----AFNKLLKNIPKKGTFDIREVLDSTYFFS 2010
             + W TP+   + QPY    + ILK                  D +K KNAFPPNFIHSLDSTHMMLT+L C    +TFVSVHDC+WTHA  VD MN+ CRE+FV LHSQPILE LS +LL+KY  +  P      +K+       K+L ++P+ G FD+ +V DS YFFS
Sbjct: 1113 TVEWVTPLGLPIIQPYHRIRNQILKGNIQNLNIHISHDANEKPDSMKQKNAFPPNFIHSLDSTHMMLTSLHCHRAGLTFVSVHDCYWTHAVTVDIMNRVCREQFVALHSQPILEELSWFLLQKYSRR--PPVGSKPKKLLEYYTLMKVLSSVPQTGDFDLEKVKDSVYFFS 1281

HSP 2 Score: 60.077 bits (144), Expect = 3.988e-51
Identity = 47/104 (45.19%), Postives = 64/104 (61.54%), Query Frame = -3

Query: 2091 QDVYSDVLEHLEVKRKEDAKNGVEEAILLEGLLSRKVIKXXXXXXXXXXXXXXXTLQILRELKNLEKLPPQNIKSCAAYLSKLTLGSIGEIFASATNIQNWFIE 2402
            QDVYS V + +E  R  DA+ G++ A +L+G +SRKV+KQTVMT VYGVT+YGG LQI + LK ++  P   +   + YL      S+ E+F     IQ W  E
Sbjct:  999 QDVYSGVAKQVEEFRVRDAERGLKIAQVLQGFISRKVVKQTVMTVVYGVTRYGGRLQIEKRLKEIDDFPKDYVWDASHYLVVQVFNSLKEMFTGTREIQEWLTE 1102

[Close]

BLAST of SMED30023981 vs. Ensembl Zebrafish
Match: kansl3 (KAT8 regulatory NSL complex subunit 3 [Source:NCBI gene;Acc:555951])

HSP 1 Score: 64.3142 bits (155), Expect = 4.514e-10
Identity = 67/327 (20.49%), Postives = 144/327 (44.04%), Query Frame = 2

Query:  518 QISKRIYYTYAASHWRMDICSTAHAYLLENLSDEYLVIYIESLRVMYHRKKVRTILDQL---------NIWAQNQKILHRFSWASLLEII-HREPEKLN---------EDLSEFSWNIENR---WK----------PIIIHIYSDRWSDSNRLQKFIKILKTFSTVVDFPFDDSGRLRKKFEALRNQLQLELKHKKSSVFVMGWDLGALLGLLSSIKIHGYFTSVFCGVICLGLPLKGLKGERGIIPEDYIHLLTKETLIISGDMSSLGNGDDIEDFKDRMKGRVDIKHINIGGCDHLLRLSSGYAMRYRISQDVVDRLIAKNIRNF 1402
            + ++++ +  ++  W + +    H  LLE+LS   L  Y++ L+ +  + KV +++D++            A+   +L +  W   + ++ H +P KL             +  ++    R   W+          PI +H+ S            + I +    ++       G +R K      ++     HK   + ++GW++G+L+     + +  Y T+V    +CLG PL+ + G RG + +D +  +    L + G  +   + +++E+F++++  R D   + +GG D  LR++S       ++Q +VDR I   I +F
Sbjct:  173 KCARKVRHALSSVSWDIKLTHWLHNTLLESLSLAMLAAYLDVLQTL--KSKVPSLIDRMLLSSSVKTGAAGAEALSLLLKRPWDPAVGVLSHNKPSKLPGSPLILIAPSGPTNPAFPTSRRMRFWQSQLSCLGKVIPINVHVGSG---------ANVGITQCLEHMI-------GTVRGKV----IEVHSHFPHKP--IILVGWNVGSLMAC--HVSLMEYMTAV----VCLGFPLQTISGPRGDV-DDPLLDMKTPVLFVVGQNALQCSPENMEEFREKI--RADNSLVVVGGADDSLRINSTKMKTEGLTQTMVDRCIQDEIVDF 466

[Close]

BLAST of SMED30023981 vs. Ensembl Zebrafish
Match: kansl3 (KAT8 regulatory NSL complex subunit 3 [Source:NCBI gene;Acc:555951])

HSP 1 Score: 64.3142 bits (155), Expect = 5.128e-10
Identity = 67/327 (20.49%), Postives = 144/327 (44.04%), Query Frame = 2

Query:  518 QISKRIYYTYAASHWRMDICSTAHAYLLENLSDEYLVIYIESLRVMYHRKKVRTILDQL---------NIWAQNQKILHRFSWASLLEII-HREPEKLN---------EDLSEFSWNIENR---WK----------PIIIHIYSDRWSDSNRLQKFIKILKTFSTVVDFPFDDSGRLRKKFEALRNQLQLELKHKKSSVFVMGWDLGALLGLLSSIKIHGYFTSVFCGVICLGLPLKGLKGERGIIPEDYIHLLTKETLIISGDMSSLGNGDDIEDFKDRMKGRVDIKHINIGGCDHLLRLSSGYAMRYRISQDVVDRLIAKNIRNF 1402
            + ++++ +  ++  W + +    H  LLE+LS   L  Y++ L+ +  + KV +++D++            A+   +L +  W   + ++ H +P KL             +  ++    R   W+          PI +H+ S            + I +    ++       G +R K      ++     HK   + ++GW++G+L+     + +  Y T+V    +CLG PL+ + G RG + +D +  +    L + G  +   + +++E+F++++  R D   + +GG D  LR++S       ++Q +VDR I   I +F
Sbjct:  173 KCARKVRHALSSVSWDIKLTHWLHNTLLESLSLAMLAAYLDVLQTL--KSKVPSLIDRMLLSSSVKTGAAGAEALSLLLKRPWDPAVGVLSHNKPSKLPGSPLILIAPSGPTNPAFPTSRRMRFWQSQLSCLGKVIPINVHVGSG---------ANVGITQCLEHMI-------GTVRGKV----IEVHSHFPHKP--IILVGWNVGSLMAC--HVSLMEYMTAV----VCLGFPLQTISGPRGDV-DDPLLDMKTPVLFVVGQNALQCSPENMEEFREKI--RADNSLVVVGGADDSLRINSTKMKTEGLTQTMVDRCIQDEIVDF 466

[Close]

BLAST of SMED30023981 vs. Ensembl Xenopus
Match: pacrg (parkin co-regulated [Source:Xenbase;Acc:XB-GENE-6258806])

HSP 1 Score: 165.622 bits (418), Expect = 3.931e-53
Identity = 84/172 (48.84%), Postives = 108/172 (62.79%), Query Frame = -2

Query: 1561 NISWKTPMNFIVKQPYFADDSDILKS-----------------DCLKNKNAFPPNFIHSLDSTHMMLTALSCWEKNITFVSVHDCFWTHACDVDEMNKFCREEFVKLHSQPILENLSDYLLEKYQFQINPNENDNGRKIAFNKL-----LKNIPKKGTFDIREVLDSTYFFS 2010
             + W+TP+   + QPY      + +S                 D +K KNAFPPNFIHSLDSTHMMLTAL C+   +TFVSVHDCFWTH   VD MNK CRE+FV LHSQPIL+NLS +LL+KY   ++   +D   K++   L       N+P+ G FD+++V+DSTYFFS
Sbjct: 1040 TVEWQTPLGLPIIQPYHRTKPVLFQSNLQVVNLQSSHDVNERPDTMKQKNAFPPNFIHSLDSTHMMLTALHCYSHGLTFVSVHDCFWTHPDTVDVMNKVCREQFVALHSQPILQNLSQFLLQKYCQTLS---SDKMAKMSPENLRMTLHFSNVPETGNFDLKQVIDSTYFFS 1208

HSP 2 Score: 64.6994 bits (156), Expect = 3.931e-53
Identity = 48/104 (46.15%), Postives = 67/104 (64.42%), Query Frame = -3

Query: 2091 QDVYSDVLEHLEVKRKEDAKNGVEEAILLEGLLSRKVIKXXXXXXXXXXXXXXXTLQILRELKNLEKLPPQNIKSCAAYLSKLTLGSIGEIFASATNIQNWFIE 2402
            QDVYS V + +E  RK DA+ GV+ A +L+G + RKV+KQTVMT VYGVT+YGG LQI + L+ L+  P + +   + YL +    S+ E+F+    IQ W  E
Sbjct:  926 QDVYSGVAQQVEEFRKRDAQRGVKIAQILDGFIGRKVVKQTVMTVVYGVTRYGGRLQIEKRLRELDNFPKEYVWEASHYLVQQVFSSLKEMFSGTREIQLWLTE 1029

[Close]

BLAST of SMED30023981 vs. Ensembl Xenopus
Match: pacrg (parkin co-regulated [Source:Xenbase;Acc:XB-GENE-6258806])

HSP 1 Score: 165.622 bits (418), Expect = 4.488e-53
Identity = 84/172 (48.84%), Postives = 108/172 (62.79%), Query Frame = -2

Query: 1561 NISWKTPMNFIVKQPYFADDSDILKS-----------------DCLKNKNAFPPNFIHSLDSTHMMLTALSCWEKNITFVSVHDCFWTHACDVDEMNKFCREEFVKLHSQPILENLSDYLLEKYQFQINPNENDNGRKIAFNKL-----LKNIPKKGTFDIREVLDSTYFFS 2010
             + W+TP+   + QPY      + +S                 D +K KNAFPPNFIHSLDSTHMMLTAL C+   +TFVSVHDCFWTH   VD MNK CRE+FV LHSQPIL+NLS +LL+KY   ++   +D   K++   L       N+P+ G FD+++V+DSTYFFS
Sbjct: 1059 TVEWQTPLGLPIIQPYHRTKPVLFQSNLQVVNLQSSHDVNERPDTMKQKNAFPPNFIHSLDSTHMMLTALHCYSHGLTFVSVHDCFWTHPDTVDVMNKVCREQFVALHSQPILQNLSQFLLQKYCQTLS---SDKMAKMSPENLRMTLHFSNVPETGNFDLKQVIDSTYFFS 1227

HSP 2 Score: 64.3142 bits (155), Expect = 4.488e-53
Identity = 48/104 (46.15%), Postives = 67/104 (64.42%), Query Frame = -3

Query: 2091 QDVYSDVLEHLEVKRKEDAKNGVEEAILLEGLLSRKVIKXXXXXXXXXXXXXXXTLQILRELKNLEKLPPQNIKSCAAYLSKLTLGSIGEIFASATNIQNWFIE 2402
            QDVYS V + +E  RK DA+ GV+ A +L+G + RKV+KQTVMT VYGVT+YGG LQI + L+ L+  P + +   + YL +    S+ E+F+    IQ W  E
Sbjct:  945 QDVYSGVAQQVEEFRKRDAQRGVKIAQILDGFIGRKVVKQTVMTVVYGVTRYGGRLQIEKRLRELDNFPKEYVWEASHYLVQQVFSSLKEMFSGTREIQLWLTE 1048

[Close]

BLAST of SMED30023981 vs. Ensembl Xenopus
Match: pacrg (parkin co-regulated [Source:Xenbase;Acc:XB-GENE-6258806])

HSP 1 Score: 153.68 bits (387), Expect = 1.773e-49
Identity = 81/165 (49.09%), Postives = 102/165 (61.82%), Query Frame = -2

Query: 1561 NISWKTPMNFIVKQPY--------------FADDSDI-LKSDCLKNKNAFPPNFIHSLDSTHMMLTALSCWEKNITFVSVHDCFWTHACDVDEMNKFCREEFVKLHSQPILENLSDYLLEKYQFQINPNENDNGRKIAFNKLLKNIPKKGTFDIREVLDSTYFFS 2010
             + W+TP+   + QPY                   D+  + D +K KNAFPPNFIHSLDSTHMMLTAL C+   +TFVSVHDCFWTH   VD MNK CRE+FV LHSQPIL+NLS +LL+KY   ++       R +    L   +   G FD+++V+DSTYFFS
Sbjct: 1034 TVEWQTPLGLPIIQPYHRTKPVLSNLQVVNLQSSHDVNERPDTMKQKNAFPPNFIHSLDSTHMMLTALHCYSHGLTFVSVHDCFWTHPDTVDVMNKVCREQFVALHSQPILQNLSQFLLQKYCQTLS-------RPMIILFLCFIV---GNFDLKQVIDSTYFFS 1188

HSP 2 Score: 64.6994 bits (156), Expect = 1.773e-49
Identity = 48/104 (46.15%), Postives = 67/104 (64.42%), Query Frame = -3

Query: 2091 QDVYSDVLEHLEVKRKEDAKNGVEEAILLEGLLSRKVIKXXXXXXXXXXXXXXXTLQILRELKNLEKLPPQNIKSCAAYLSKLTLGSIGEIFASATNIQNWFIE 2402
            QDVYS V + +E  RK DA+ GV+ A +L+G + RKV+KQTVMT VYGVT+YGG LQI + L+ L+  P + +   + YL +    S+ E+F+    IQ W  E
Sbjct:  920 QDVYSGVAQQVEEFRKRDAQRGVKIAQILDGFIGRKVVKQTVMTVVYGVTRYGGRLQIEKRLRELDNFPKEYVWEASHYLVQQVFSSLKEMFSGTREIQLWLTE 1023

[Close]

BLAST of SMED30023981 vs. Ensembl Xenopus
Match: pacrg (parkin co-regulated [Source:Xenbase;Acc:XB-GENE-6258806])

HSP 1 Score: 152.525 bits (384), Expect = 2.890e-49
Identity = 79/163 (48.47%), Postives = 102/163 (62.58%), Query Frame = -2

Query: 1561 NISWKTPMNFIVKQPYFADD-------------SDILKSDCLKNKNAFPPNFIHSLDSTHMMLTALSCWEKNITFVSVHDCFWTHACDVDEMNKFCREEFVKLHSQPILENLSDYLLEKYQFQINPNENDNGRKIAFNKLLKNIPKKGTFDIREVLDSTYFFS 2010
             + W+TP+   + QPY                    ++++ +K KNAFPPNFIHSLDSTHMMLTAL C+   +TFVSVHDCFWTH   VD MNK CRE+FV LHSQPIL+NLS +LL+KY   +    +   R +    L   +   G FD+++V+DSTYFFS
Sbjct: 1007 TVEWQTPLGLPIIQPYHRTKPVLVSYVLLHCMCGATMQNNTMKQKNAFPPNFIHSLDSTHMMLTALHCYSHGLTFVSVHDCFWTHPDTVDVMNKVCREQFVALHSQPILQNLSQFLLQKYCQTL----STWSRPMIILFLCFIV---GNFDLKQVIDSTYFFS 1162

HSP 2 Score: 65.0846 bits (157), Expect = 2.890e-49
Identity = 48/104 (46.15%), Postives = 67/104 (64.42%), Query Frame = -3

Query: 2091 QDVYSDVLEHLEVKRKEDAKNGVEEAILLEGLLSRKVIKXXXXXXXXXXXXXXXTLQILRELKNLEKLPPQNIKSCAAYLSKLTLGSIGEIFASATNIQNWFIE 2402
            QDVYS V + +E  RK DA+ GV+ A +L+G + RKV+KQTVMT VYGVT+YGG LQI + L+ L+  P + +   + YL +    S+ E+F+    IQ W  E
Sbjct:  893 QDVYSGVAQQVEEFRKRDAQRGVKIAQILDGFIGRKVVKQTVMTVVYGVTRYGGRLQIEKRLRELDNFPKEYVWEASHYLVQQVFSSLKEMFSGTREIQLWLTE 996

[Close]

BLAST of SMED30023981 vs. Ensembl Xenopus
Match: pacrg (parkin co-regulated [Source:Xenbase;Acc:XB-GENE-6258806])

HSP 1 Score: 144.436 bits (363), Expect = 9.969e-47
Identity = 68/124 (54.84%), Postives = 81/124 (65.32%), Query Frame = -2

Query: 1690 NISWKTPMNFIVKQPYFADDSDILKS-----------------DCLKNKNAFPPNFIHSLDSTHMMLTALSCWEKNITFVSVHDCFWTHACDVDEMNKFCREEFVKLHSQPILENLSDYLLEKY 2010
             + W+TP+   + QPY      + +S                 D +K KNAFPPNFIHSLDSTHMMLTAL C+   +TFVSVHDCFWTH   VD MNK CRE+FV LHSQPIL+NLS +LL+KY
Sbjct: 1040 TVEWQTPLGLPIIQPYHRTKPVLFQSNLQVVNLQSSHDVNERPDTMKQKNAFPPNFIHSLDSTHMMLTALHCYSHGLTFVSVHDCFWTHPDTVDVMNKVCREQFVALHSQPILQNLSQFLLQKY 1163

HSP 2 Score: 64.3142 bits (155), Expect = 9.969e-47
Identity = 48/104 (46.15%), Postives = 67/104 (64.42%), Query Frame = -3

Query: 2091 QDVYSDVLEHLEVKRKEDAKNGVEEAILLEGLLSRKVIKXXXXXXXXXXXXXXXTLQILRELKNLEKLPPQNIKSCAAYLSKLTLGSIGEIFASATNIQNWFIE 2402
            QDVYS V + +E  RK DA+ GV+ A +L+G + RKV+KQTVMT VYGVT+YGG LQI + L+ L+  P + +   + YL +    S+ E+F+    IQ W  E
Sbjct:  926 QDVYSGVAQQVEEFRKRDAQRGVKIAQILDGFIGRKVVKQTVMTVVYGVTRYGGRLQIEKRLRELDNFPKEYVWEASHYLVQQVFSSLKEMFSGTREIQLWLTE 1029

[Close]

BLAST of SMED30023981 vs. Ensembl Mouse
Match: Polrmt (polymerase (RNA) mitochondrial (DNA directed) [Source:MGI Symbol;Acc:MGI:1915843])

HSP 1 Score: 160.614 bits (405), Expect = 2.401e-52
Identity = 77/169 (45.56%), Postives = 105/169 (62.13%), Query Frame = -2

Query: 1561 ISWKTPMNFIVKQPYFADDS-----------------DILKSDCLKNKNAFPPNFIHSLDSTHMMLTALSCWEKNITFVSVHDCFWTHACDVDEMNKFCREEFVKLHSQPILENLSDYLLEKYQFQINPNENDNGRKIAFNKL---LKNIPKKGTFDIREVLDSTYFFS 2007
            + W TP+   + QPY  +                   +  K + LK KN FPPNFIHSLDS+HMMLTAL C+ K + FVSVHDCFWTHA D+  MN+ CRE+FV+LHSQPILE+L+ +L +++    +     +  +    KL   L+++PK GTFD+ +V+ STYFFS
Sbjct: 1039 VEWVTPLGIPIIQPYHRESKVQVKGGLQSITLTSSVDESQKPNTLKQKNGFPPNFIHSLDSSHMMLTALHCYRKGLIFVSVHDCFWTHAADIPTMNEVCREQFVRLHSQPILEDLAKFLKKRFCSVSSIKSLKSSERALVTKLQETLQSLPKTGTFDLGQVIRSTYFFS 1207

HSP 2 Score: 66.6254 bits (161), Expect = 2.401e-52
Identity = 49/104 (47.12%), Postives = 67/104 (64.42%), Query Frame = -3

Query: 2091 QDVYSDVLEHLEVKRKEDAKNGVEEAILLEGLLSRKVIKXXXXXXXXXXXXXXXTLQILRELKNLEKLPPQNIKSCAAYLSKLTLGSIGEIFASATNIQNWFIE 2402
            QDVY +V   +E  R++DAK G+  A +LEG +SRKV+KQTVMT VYGVT+YGG LQI + L+ L   P + +   + YL +    S+ E+F S   IQ+W  E
Sbjct:  924 QDVYREVATQVEEFRQQDAKEGLRVAQVLEGFISRKVVKQTVMTVVYGVTRYGGRLQIEKRLRELSDFPQEFVWEASHYLVRQVFKSLQEMFTSTRAIQHWLTE 1027

[Close]

BLAST of SMED30023981 vs. Ensembl Mouse
Match: Polrmt (polymerase (RNA) mitochondrial (DNA directed) [Source:MGI Symbol;Acc:MGI:1915843])

HSP 1 Score: 160.229 bits (404), Expect = 2.766e-52
Identity = 77/169 (45.56%), Postives = 105/169 (62.13%), Query Frame = -2

Query: 1561 ISWKTPMNFIVKQPYFADDS-----------------DILKSDCLKNKNAFPPNFIHSLDSTHMMLTALSCWEKNITFVSVHDCFWTHACDVDEMNKFCREEFVKLHSQPILENLSDYLLEKYQFQINPNENDNGRKIAFNKL---LKNIPKKGTFDIREVLDSTYFFS 2007
            + W TP+   + QPY  +                   +  K + LK KN FPPNFIHSLDS+HMMLTAL C+ K + FVSVHDCFWTHA D+  MN+ CRE+FV+LHSQPILE+L+ +L +++    +     +  +    KL   L+++PK GTFD+ +V+ STYFFS
Sbjct:  965 VEWVTPLGIPIIQPYHRESKVQVKGGLQSITLTSSVDESQKPNTLKQKNGFPPNFIHSLDSSHMMLTALHCYRKGLIFVSVHDCFWTHAADIPTMNEVCREQFVRLHSQPILEDLAKFLKKRFCSVSSIKSLKSSERALVTKLQETLQSLPKTGTFDLGQVIRSTYFFS 1133

HSP 2 Score: 66.6254 bits (161), Expect = 2.766e-52
Identity = 49/104 (47.12%), Postives = 67/104 (64.42%), Query Frame = -3

Query: 2091 QDVYSDVLEHLEVKRKEDAKNGVEEAILLEGLLSRKVIKXXXXXXXXXXXXXXXTLQILRELKNLEKLPPQNIKSCAAYLSKLTLGSIGEIFASATNIQNWFIE 2402
            QDVY +V   +E  R++DAK G+  A +LEG +SRKV+KQTVMT VYGVT+YGG LQI + L+ L   P + +   + YL +    S+ E+F S   IQ+W  E
Sbjct:  850 QDVYREVATQVEEFRQQDAKEGLRVAQVLEGFISRKVVKQTVMTVVYGVTRYGGRLQIEKRLRELSDFPQEFVWEASHYLVRQVFKSLQEMFTSTRAIQHWLTE 953

[Close]

BLAST of SMED30023981 vs. Ensembl Mouse
Match: Polrmt (polymerase (RNA) mitochondrial (DNA directed) [Source:MGI Symbol;Acc:MGI:1915843])

HSP 1 Score: 158.688 bits (400), Expect = 4.507e-49
Identity = 77/169 (45.56%), Postives = 105/169 (62.13%), Query Frame = -2

Query: 1561 ISWKTPMNFIVKQPYFADDSDILKS-----------------DCLKNKNAFPPNFIHSLDSTHMMLTALSCWEKNITFVSVHDCFWTHACDVDEMNKFCREEFVKLHSQPILENLSDYLLEKYQFQINPNENDNGRKIAFNKL---LKNIPKKGTFDIREVLDSTYFFS 2007
            + W TP+   + QPY  +    +K                  + LK KN FPPNFIHSLDS+HMMLTAL C+ K + FVSVHDCFWTHA D+  MN+ CRE+FV+LHSQPILE+L+ +L +++    +     +  +    KL   L+++PK GTFD+ +V+ STYFFS
Sbjct:  225 VEWVTPLGIPIIQPYHRESKVQVKGGLQSITLTSSVDESQKPNTLKQKNGFPPNFIHSLDSSHMMLTALHCYRKGLIFVSVHDCFWTHAADIPTMNEVCREQFVRLHSQPILEDLAKFLKKRFCSVSSIKSLKSSERALVTKLQETLQSLPKTGTFDLGQVIRSTYFFS 393

HSP 2 Score: 57.3806 bits (137), Expect = 4.507e-49
Identity = 46/102 (45.10%), Postives = 63/102 (61.76%), Query Frame = -3

Query: 2091 LEHLEVK-----RKEDAKNGVEEAILLEGLLSRKVIKXXXXXXXXXXXXXXXTLQILRELKNLEKLPPQNIKSCAAYLSKLTLGSIGEIFASATNIQNWFIE 2381
            + HL V      R++DAK G+  A +LEG +SRKV+KQTVMT VYGVT+YGG LQI + L+ L   P + +   + YL +    S+ E+F S   IQ+W  E
Sbjct:  112 ISHLPVHQVEEFRQQDAKEGLRVAQVLEGFISRKVVKQTVMTVVYGVTRYGGRLQIEKRLRELSDFPQEFVWEASHYLVRQVFKSLQEMFTSTRAIQHWLTE 213

[Close]

BLAST of SMED30023981 vs. Ensembl Mouse
Match: Kansl3 (KAT8 regulatory NSL complex subunit 3 [Source:MGI Symbol;Acc:MGI:1918055])

HSP 1 Score: 68.9366 bits (167), Expect = 1.829e-11
Identity = 74/322 (22.98%), Postives = 144/322 (44.72%), Query Frame = 2

Query:  518 QISKRIYYTYAASHWRMDICSTAHAYLLENLSDEYLVIYIESLRVMYHRKKVRTILDQLNI---------WAQNQKILHRFSWASLLEII-HREPEKLNEDLSEFSWNIENRWKPIIIHIYSDRWS---DSNRLQKFIK-----ILKTFSTVVDFPFDDSGR-----LRKKFEALRNQLQLEL----KHKKSSVFVMGWDLGALLGLLSSIKIHGYFTSVFCGVICLGLPLKGLKGERGIIPEDYIHLLTKETLIISGDMSSLGNGDDIEDFKDRMKGRVDIKHINIGGCDHLLRLSSGYAMRYRISQDVVDRLIAKNIRNF 1402
            + ++R+    A+  W   +    H  L+E LS   L  Y+++L+ +  + K+ T++D++ +          A+   +L +  W   + ++ H +P KL                P+I+ + S   S    ++R  +F +     + K          + SG      L     A+R+++ LE+     HK   + ++GW+ GAL+     + +  Y T+V    +CLG PL  + G RG + +D +  +    L + G  S   + + +EDF++++  R +   + +GG D  LR+S        ++Q +VDR I   I +F
Sbjct:  173 KCARRVRQALASVSWDTKLTQWLHTTLVETLSLPMLAAYLDALQTL--KGKIPTLIDRMLVSSNTKTGAAGAEALSLLLKRPWDPAVGVLSHNKPSKLP-------------GSPLILIVSSGPSSSVFPASRRHRFWQSQLSCLGKVIPVATHLLNNGSGVGVLQCLEHMIGAVRSKV-LEIHSHFPHKP--IILIGWNTGALVAC--HVSVMEYVTAV----VCLGFPLLTVDGPRGDV-DDPLLDMKTPVLFVIGQNSLQCHPEAMEDFREKI--RAENSLVVVGGADDNLRISKAKKKSEGLTQSMVDRCIQDEIVDF 467

[Close]

BLAST of SMED30023981 vs. Ensembl Mouse
Match: Kansl3 (KAT8 regulatory NSL complex subunit 3 [Source:MGI Symbol;Acc:MGI:1918055])

HSP 1 Score: 68.5514 bits (166), Expect = 2.158e-11
Identity = 74/322 (22.98%), Postives = 144/322 (44.72%), Query Frame = 2

Query:  518 QISKRIYYTYAASHWRMDICSTAHAYLLENLSDEYLVIYIESLRVMYHRKKVRTILDQLNI---------WAQNQKILHRFSWASLLEII-HREPEKLNEDLSEFSWNIENRWKPIIIHIYSDRWS---DSNRLQKFIK-----ILKTFSTVVDFPFDDSGR-----LRKKFEALRNQLQLEL----KHKKSSVFVMGWDLGALLGLLSSIKIHGYFTSVFCGVICLGLPLKGLKGERGIIPEDYIHLLTKETLIISGDMSSLGNGDDIEDFKDRMKGRVDIKHINIGGCDHLLRLSSGYAMRYRISQDVVDRLIAKNIRNF 1402
            + ++R+    A+  W   +    H  L+E LS   L  Y+++L+ +  + K+ T++D++ +          A+   +L +  W   + ++ H +P KL                P+I+ + S   S    ++R  +F +     + K          + SG      L     A+R+++ LE+     HK   + ++GW+ GAL+     + +  Y T+V    +CLG PL  + G RG + +D +  +    L + G  S   + + +EDF++++  R +   + +GG D  LR+S        ++Q +VDR I   I +F
Sbjct:   74 KCARRVRQALASVSWDTKLTQWLHTTLVETLSLPMLAAYLDALQTL--KGKIPTLIDRMLVSSNTKTGAAGAEALSLLLKRPWDPAVGVLSHNKPSKLP-------------GSPLILIVSSGPSSSVFPASRRHRFWQSQLSCLGKVIPVATHLLNNGSGVGVLQCLEHMIGAVRSKV-LEIHSHFPHKP--IILIGWNTGALVAC--HVSVMEYVTAV----VCLGFPLLTVDGPRGDV-DDPLLDMKTPVLFVIGQNSLQCHPEAMEDFREKI--RAENSLVVVGGADDNLRISKAKKKSEGLTQSMVDRCIQDEIVDF 368

[Close]

BLAST of SMED30023981 vs. UniProt/SwissProt
Match: sp|Q8BKF1|RPOM_MOUSE (DNA-directed RNA polymerase, mitochondrial OS=Mus musculus OX=10090 GN=Polrmt PE=1 SV=1)

HSP 1 Score: 160.614 bits (405), Expect = 1.461e-51
Identity = 77/169 (45.56%), Postives = 105/169 (62.13%), Query Frame = -2

Query: 1561 ISWKTPMNFIVKQPYFADDS-----------------DILKSDCLKNKNAFPPNFIHSLDSTHMMLTALSCWEKNITFVSVHDCFWTHACDVDEMNKFCREEFVKLHSQPILENLSDYLLEKYQFQINPNENDNGRKIAFNKL---LKNIPKKGTFDIREVLDSTYFFS 2007
            + W TP+   + QPY  +                   +  K + LK KN FPPNFIHSLDS+HMMLTAL C+ K + FVSVHDCFWTHA D+  MN+ CRE+FV+LHSQPILE+L+ +L +++    +     +  +    KL   L+++PK GTFD+ +V+ STYFFS
Sbjct: 1039 VEWVTPLGIPIIQPYHRESKVQVKGGLQSITLTSSVDESQKPNTLKQKNGFPPNFIHSLDSSHMMLTALHCYRKGLIFVSVHDCFWTHAADIPTMNEVCREQFVRLHSQPILEDLAKFLKKRFCSVSSIKSLKSSERALVTKLQETLQSLPKTGTFDLGQVIRSTYFFS 1207

HSP 2 Score: 66.6254 bits (161), Expect = 1.461e-51
Identity = 49/104 (47.12%), Postives = 67/104 (64.42%), Query Frame = -3

Query: 2091 QDVYSDVLEHLEVKRKEDAKNGVEEAILLEGLLSRKVIKXXXXXXXXXXXXXXXTLQILRELKNLEKLPPQNIKSCAAYLSKLTLGSIGEIFASATNIQNWFIE 2402
            QDVY +V   +E  R++DAK G+  A +LEG +SRKV+KQTVMT VYGVT+YGG LQI + L+ L   P + +   + YL +    S+ E+F S   IQ+W  E
Sbjct:  924 QDVYREVATQVEEFRQQDAKEGLRVAQVLEGFISRKVVKQTVMTVVYGVTRYGGRLQIEKRLRELSDFPQEFVWEASHYLVRQVFKSLQEMFTSTRAIQHWLTE 1027

[Close]

BLAST of SMED30023981 vs. UniProt/SwissProt
Match: sp|O00411|RPOM_HUMAN (DNA-directed RNA polymerase, mitochondrial OS=Homo sapiens OX=9606 GN=POLRMT PE=1 SV=2)

HSP 1 Score: 157.532 bits (397), Expect = 7.914e-51
Identity = 79/167 (47.31%), Postives = 105/167 (62.87%), Query Frame = -2

Query: 1561 ISWKTPMNFIVKQPYFAD-----------------DSDI-LKSDCLKNKNAFPPNFIHSLDSTHMMLTALSCWEKNITFVSVHDCFWTHACDVDEMNKFCREEFVKLHSQPILENLSDYLLEKYQFQINPNENDNGRKIAFNKLLKNIPKKGTFDIREVLDSTYFFS 2007
            + W TP+   V QPY  D                 + DI  K +  K KN FPPNFIHSLDS+HMMLTAL C+ K +TFVSVHDC+WTHA DV  MN+ CRE+FV+LHS+PIL++LS +L+++  F   P +     ++   + L+ +PK G FD+ +V  STYFFS
Sbjct: 1068 VEWVTPLGVPVIQPYRLDSKVKQIGGGIQSITYTHNGDISRKPNTRKQKNGFPPNFIHSLDSSHMMLTALHCYRKGLTFVSVHDCYWTHAADVSVMNQVCREQFVRLHSEPILQDLSRFLVKR--FCSEPQKILEASQL--KETLQAVPKPGAFDLEQVKRSTYFFS 1230

HSP 2 Score: 67.0106 bits (162), Expect = 7.914e-51
Identity = 48/104 (46.15%), Postives = 68/104 (65.38%), Query Frame = -3

Query: 2091 QDVYSDVLEHLEVKRKEDAKNGVEEAILLEGLLSRKVIKXXXXXXXXXXXXXXXTLQILRELKNLEKLPPQNIKSCAAYLSKLTLGSIGEIFASATNIQNWFIE 2402
            QDVYS V   +EV R++DA+ G+  A +LEG ++RKV+KQTVMT VYGVT+YGG LQI + L+ L   P + +   + YL +    S+ E+F+    IQ+W  E
Sbjct:  953 QDVYSGVAAQVEVFRRQDAQRGMRVAQVLEGFITRKVVKQTVMTVVYGVTRYGGRLQIEKRLRELSDFPQEFVWEASHYLVRQVFKSLQEMFSGTRAIQHWLTE 1056

[Close]

BLAST of SMED30023981 vs. UniProt/SwissProt
Match: sp|O24600|RPOT3_ARATH (DNA-directed RNA polymerase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=RPOT3 PE=2 SV=1)

HSP 1 Score: 138.272 bits (347), Expect = 1.618e-35
Identity = 66/162 (40.74%), Postives = 91/162 (56.17%), Query Frame = -2

Query: 1564 ISWKTPMNFIVKQPYFADDSDILKS--------------DCLKNKNAFPPNFIHSLDSTHMMLTALSCWEKNITFVSVHDCFWTHACDVDEMNKFCREEFVKLHSQPILENLSDYLLEKYQFQINPNENDNGRKIAFNKLLKNIPKKGTFDIREVLDSTYFF 2007
            + W TP+   V QPY   +  ++++              D  K + AFPPNF+HSLD THMM+TA++C E  + F  VHD +WTHACDVD MN+  RE+FV+L++ PILE+L     E Y                 N +   +PK+G FD++EVL S YFF
Sbjct:  847 VRWITPLGLPVVQPYCRSERHLIRTSLQVLALQREGNTVDVRKQRTAFPPNFVHSLDGTHMMMTAVACREAGLNFAGVHDSYWTHACDVDTMNRILREKFVELYNTPILEDLLQSFQESYP----------------NLVFPPVPKRGDFDLKEVLKSQYFF 992

HSP 2 Score: 35.039 bits (79), Expect = 1.618e-35
Identity = 40/108 (37.04%), Postives = 60/108 (55.56%), Query Frame = -3

Query: 2097 DVYSDVLEHLEVKRKEDAKNGVEE------AILLEGLLSRKVIKXXXXXXXXXXXXXXXTLQILRELKNLEKLPPQNIKSCAA-YLSKLTLGSIGEIFASATNIQNWF 2399
            DVYS++   +    K+D+    E       A +L   + RK++KQTVMT+VYGVT  G   QI R L+    +  + +   AA Y +K+TL ++GEIF +A  I +W 
Sbjct:  726 DVYSEISRRVHEIMKKDSSKDPESNPTAALAKILITQVDRKLVKQTVMTSVYGVTYVGAREQIKRRLEEKGVITDERMLFAAACYSAKVTLAALGEIFEAARAIMSWL 833

[Close]

BLAST of SMED30023981 vs. UniProt/SwissProt
Match: sp|Q8L6J5|RPO1B_TOBAC (DNA-directed RNA polymerase 1B, mitochondrial OS=Nicotiana tabacum OX=4097 GN=RPOT1-TOM PE=2 SV=2)

HSP 1 Score: 136.732 bits (343), Expect = 1.862e-35
Identity = 70/162 (43.21%), Postives = 96/162 (59.26%), Query Frame = -2

Query: 1564 ISWKTPMNFIVKQPYFADDSDILKSD--------------CLKNKNAFPPNFIHSLDSTHMMLTALSCWEKNITFVSVHDCFWTHACDVDEMNKFCREEFVKLHSQPILENLSDYLLEKYQFQINPNENDNGRKIAFNKLLKNIPKKGTFDIREVLDSTYFF 2007
            + W TP+   V QPY      ++K+                 + + AFPPNF+HSLD +HMM+TA++C E  ++F  VHD +WTHACDVD+MNK  RE+FV+L+  PILEN    LLE +Q Q  P+       + F  L    P++G FD+REVL+S YFF
Sbjct:  856 VRWTTPLGLPVVQPYRKLGRHLIKTSLQILTLQRETDKVMVKRQRTAFPPNFVHSLDGSHMMMTAIACKESGLSFAGVHDSYWTHACDVDQMNKILREKFVELYDAPILEN----LLESFQ-QSFPD-------LQFPPL----PERGDFDLREVLESPYFF 1001

HSP 2 Score: 36.5798 bits (83), Expect = 1.862e-35
Identity = 41/108 (37.96%), Postives = 63/108 (58.33%), Query Frame = -3

Query: 2097 DVYSDVLEH-LEVKRKEDAKN-----GVEEAILLEGLLSRKVIKXXXXXXXXXXXXXXXTLQILRELKNLEKLPPQN-IKSCAAYLSKLTLGSIGEIFASATNIQNWF 2399
            DVYS +    L++ +++ AK+      V  A LL   + RK++KQTVMT+VYGVT  G   QI + LK    +   N + + A Y +K TL ++GE+F +A +I +W 
Sbjct:  735 DVYSGIAARVLDIMKRDAAKDPANDPNVMRARLLINQVDRKLVKQTVMTSVYGVTYIGARDQIKKRLKERGVIEDDNELFAAACYAAKTTLTALGEMFEAARSIMSWL 842

[Close]

BLAST of SMED30023981 vs. UniProt/SwissProt
Match: sp|Q93Y94|RPOT1_NICSY (DNA-directed RNA polymerase 1, mitochondrial OS=Nicotiana sylvestris OX=4096 GN=RPOT1 PE=2 SV=1)

HSP 1 Score: 132.88 bits (333), Expect = 9.956e-35
Identity = 69/162 (42.59%), Postives = 95/162 (58.64%), Query Frame = -2

Query: 1564 ISWKTPMNFIVKQPYFADDSDILKSD--------------CLKNKNAFPPNFIHSLDSTHMMLTALSCWEKNITFVSVHDCFWTHACDVDEMNKFCREEFVKLHSQPILENLSDYLLEKYQFQINPNENDNGRKIAFNKLLKNIPKKGTFDIREVLDSTYFF 2007
            + W TP+   V QPY      ++K+                 + + AFPPNF+HSLD +HMM+TA++C E  ++F  VHD +WTHA DVD+MNK  RE+FV+L+  PILEN    LLE +Q Q  P+       + F  L    P++G FD+REVL+S YFF
Sbjct:  856 VRWTTPLGLPVVQPYRKLGRHLIKTSLQILTLQRETDKVMVKRQRTAFPPNFVHSLDGSHMMMTAIACKESGLSFAGVHDSYWTHASDVDQMNKILREKFVELYDAPILEN----LLESFQ-QSFPD-------LQFPPL----PERGDFDLREVLESPYFF 1001

HSP 2 Score: 37.7354 bits (86), Expect = 9.956e-35
Identity = 42/108 (38.89%), Postives = 63/108 (58.33%), Query Frame = -3

Query: 2097 DVYSDVLEH-LEVKRKEDAKN-----GVEEAILLEGLLSRKVIKXXXXXXXXXXXXXXXTLQILRELKNLEKLPPQN-IKSCAAYLSKLTLGSIGEIFASATNIQNWF 2399
            DVYS +    L++ +++ AK+      V  A LL   + RK++KQTVMT+VYGVT  G   QI R LK    +   N + + A Y +K TL ++GE+F +A +I +W 
Sbjct:  735 DVYSGIAARVLDIMKRDAAKDPANDPNVMRARLLINQVDRKLVKQTVMTSVYGVTYIGARDQIKRRLKERGVIEDDNELFAAACYAAKTTLTALGEMFEAARSIMSWL 842

[Close]

BLAST of SMED30023981 vs. TrEMBL
Match: A0A034WBI1 (DNA-directed RNA polymerase (Fragment) OS=Bactrocera dorsalis OX=27457 GN=RPOM PE=3 SV=1)

HSP 1 Score: 178.718 bits (452), Expect = 7.922e-59
Identity = 90/178 (50.56%), Postives = 114/178 (64.04%), Query Frame = -2

Query: 1561 ISRLRKANISWKTPMNFIVKQPYFADD-----------SDIL------KSDCLKNKNAFPPNFIHSLDSTHMMLTALSCWEKNITFVSVHDCFWTHACDVDEMNKFCREEFVKLHSQPILENLSDYLLEKYQFQ----INPNENDNGRKIAFNKLLKNIPKKGTFDIREVLDSTYFFS 2031
            IS +   N+ W TP+   V QPY   +           S+ +      K + +K KNAFPPNFIHSLDS+HMMLT+L C +K +TFVSVHDCFWTHAC V EMN+ CRE+FV LHSQPILE+LS ++  KY F+    +N    D+  K   N+ L  +PKKG FD+R VL+S YFFS
Sbjct:  647 ISGVCGQNVEWVTPLGLPVVQPYIRQEVKQTMRTAARVSETMAMDMYEKPNVMKQKNAFPPNFIHSLDSSHMMLTSLYCEQKGLTFVSVHDCFWTHACTVPEMNQICREQFVSLHSQPILEDLSAFMRSKYSFRECDLLNDGSADDLSKRRLNRTLGEMPKKGDFDLRNVLNSVYFFS 824

HSP 2 Score: 80.4925 bits (197), Expect = 7.922e-59
Identity = 56/105 (53.33%), Postives = 70/105 (66.67%), Query Frame = -3

Query: 2088 QDVYSDVLEHLEVKRKEDAKNGVEEAILLEGLLSRKVIKXXXXXXXXXXXXXXXTLQILRELKNLEKLPPQNIKSCAAYLSKLTLGSIGEIFASATNIQNWFIEV 2402
            QDVYS V   +E  RK DA NGV+ A  LEG + RKVIKQTVMTTVYGVT+YG  LQI R+LK++E  P + +   + YL+  T  S+ E+F S   IQ+WF E 
Sbjct:  539 QDVYSAVAALVEKSRKSDASNGVQVAQALEGFVRRKVIKQTVMTTVYGVTRYGARLQIARQLKDIESFPKEWVWPASTYLTSKTFESLREMFTSTREIQDWFTEC 643

[Close]

BLAST of SMED30023981 vs. TrEMBL
Match: A0A1A9UTY2 (DNA-directed RNA polymerase OS=Glossina austeni OX=7395 PE=3 SV=1)

HSP 1 Score: 182.185 bits (461), Expect = 1.434e-58
Identity = 90/173 (52.02%), Postives = 113/173 (65.32%), Query Frame = -2

Query: 1561 ISRLRKANISWKTPMNFIVKQPYFADDSDIL------------KSDCLKNKNAFPPNFIHSLDSTHMMLTALSCWEKNITFVSVHDCFWTHACDVDEMNKFCREEFVKLHSQPILENLSDYLLEKYQFQ----INPNENDNGRKIAFNKLLKNIPKKGTFDIREVLDSTYFFS 2031
            IS +   N+ W TP+   V QPY   D  I             + + +K KNAFPPNFIHSLDS+HMMLT+L C  + +TFVSVHDCFWTHAC V E+NK CRE+FV LHSQPILE+LS+YL + Y F+     N    ++G K   N++L  IPKKG FD++ VL+S YFFS
Sbjct: 1192 ISGVCGKNVEWITPLGLPVVQPYNRQDKQISSRIFNASTDMYERPNVMKQKNAFPPNFIHSLDSSHMMLTSLHCEREGLTFVSVHDCFWTHACTVPELNKICREQFVSLHSQPILEDLSEYLKKTYSFKPSDFANDGSAEDGSKRRSNRILGQIPKKGDFDLQSVLNSVYFFS 1364

HSP 2 Score: 76.2554 bits (186), Expect = 1.434e-58
Identity = 54/108 (50.00%), Postives = 74/108 (68.52%), Query Frame = -3

Query: 2079 QDVYSDVLEHLEVKRKEDAKNGVEEAILLEGLLSRKVIKXXXXXXXXXXXXXXXTLQILRELKNLEKLPPQNIKSCAAYLSKLTLGSIGEIFASATNIQNWFIEVLIV 2402
            QDVYS V+  +E  RKEDAK G++ A +L+G + RKVIKQTVMTTVYGVT++G  LQI R+LK+++  P   +   ++YL+  T  S+ E+F S   IQ+WF E   V
Sbjct: 1084 QDVYSGVVVLVEKARKEDAKAGLQIAEVLKGFIKRKVIKQTVMTTVYGVTRHGARLQIARQLKDIDDFPNDWVWPASSYLTTKTFLSLREMFTSTREIQDWFTECARV 1191

[Close]

BLAST of SMED30023981 vs. TrEMBL
Match: A0A0K8VPA8 (DNA-directed RNA polymerase OS=Bactrocera latifrons OX=174628 GN=Polrmt_2 PE=3 SV=1)

HSP 1 Score: 179.104 bits (453), Expect = 2.627e-58
Identity = 91/178 (51.12%), Postives = 115/178 (64.61%), Query Frame = -2

Query: 1561 ISRLRKANISWKTPMNFIVKQPY-----------FADDSDIL------KSDCLKNKNAFPPNFIHSLDSTHMMLTALSCWEKNITFVSVHDCFWTHACDVDEMNKFCREEFVKLHSQPILENLSDYLLEKYQFQ----INPNENDNGRKIAFNKLLKNIPKKGTFDIREVLDSTYFFS 2031
            IS +   N+ W TP+   V QPY            A  S+ +      K + +K KNAFPPNFIHSLDS+HMMLT+L C +K++TFVSVHDCFWTHAC V EMN+ CRE+FV LHSQPILE+LS ++  KY F+    +N    D+  K   N+ L+ +PKKG FD+R VL S YFFS
Sbjct:  290 ISGVCGQNVEWVTPLGLPVVQPYNRQEVKQTMRTAARVSETMAMDMYEKPNVMKQKNAFPPNFIHSLDSSHMMLTSLYCEQKDVTFVSVHDCFWTHACTVSEMNQICREQFVSLHSQPILEDLSAFMRSKYSFRECDLLNDGSADDLSKRRLNRTLREMPKKGDFDLRNVLKSVYFFS 467

HSP 2 Score: 78.5666 bits (192), Expect = 2.627e-58
Identity = 55/105 (52.38%), Postives = 69/105 (65.71%), Query Frame = -3

Query: 2088 QDVYSDVLEHLEVKRKEDAKNGVEEAILLEGLLSRKVIKXXXXXXXXXXXXXXXTLQILRELKNLEKLPPQNIKSCAAYLSKLTLGSIGEIFASATNIQNWFIEV 2402
            QDVYS V   +E  RK DA  GV+ A  LEG + RKVIKQTVMTTVYGVT+YG  LQI R+LK++E  P + +   + YL+  T  S+ E+F S   IQ+WF E 
Sbjct:  182 QDVYSAVAALVEKSRKSDASKGVQVAQALEGFVRRKVIKQTVMTTVYGVTRYGARLQIARQLKDIESFPKEWVWPASTYLTSKTFESLREMFTSTREIQDWFTEC 286

[Close]

BLAST of SMED30023981 vs. TrEMBL
Match: A0A0P5SZN7 (DNA-directed RNA polymerase OS=Daphnia magna OX=35525 GN=APZ42_013396 PE=3 SV=1)

HSP 1 Score: 177.178 bits (448), Expect = 5.778e-58
Identity = 82/162 (50.62%), Postives = 113/162 (69.75%), Query Frame = -2

Query: 1561 ISRLRKANISWKTPMNFIVKQPYFADDSD-ILKSDCLKNKNAFPPNFIHSLDSTHMMLTALSCWEKNITFVSVHDCFWTHACDVDEMNKFCREEFVKLHSQPILENLSDYLLEKYQFQ---INPNENDNG-RKIAFNKLLKNIPKKGTFDIREVLDSTYFFS 2031
            IS +   N+ W TP+   V QPY  +  D   + + +K +NAFPPNFIHSLDS+HMMLTAL C  K ITF S+HDCFWTH C V+EMNK CR++FV LHSQPILE+LS +++ ++  +   I  +E+  G  +   N++L+ +P++G FD+R VL+S YFFS
Sbjct: 1023 ISTVAGENVEWITPVGLPVVQPYNKNPYDETSRPNVMKQRNAFPPNFIHSLDSSHMMLTALHCQRKGITFASIHDCFWTHPCTVNEMNKICRQQFVSLHSQPILEDLSKFMVSQFASRASDIAEDESPKGLARHRLNQILREVPRRGVFDLRLVLESVYFFS 1184

HSP 2 Score: 78.9518 bits (193), Expect = 5.778e-58
Identity = 58/104 (55.77%), Postives = 73/104 (70.19%), Query Frame = -3

Query: 2091 QDVYSDVLEHLEVKRKEDAKNGVEEAILLEGLLSRKVIKXXXXXXXXXXXXXXXTLQILRELKNLEKLPPQNIKSCAAYLSKLTLGSIGEIFASATNIQNWFIE 2402
            QDVYS+V   +E +R +DA N VE A LL G +SRKVIKQTVMTTVYGVT+YG  LQI ++LK L  L P+ +   A+YL+  T  S+ E+F SA  IQ+WF E
Sbjct:  915 QDVYSNVATLVERERAKDAANDVEIAQLLAGQISRKVIKQTVMTTVYGVTRYGARLQIAKQLKELPGLSPEQVNIGASYLADKTFQSLQEMFTSAKEIQDWFTE 1018

[Close]

BLAST of SMED30023981 vs. TrEMBL
Match: A0A0A1X790 (DNA-directed RNA polymerase OS=Zeugodacus cucurbitae OX=28588 GN=POLRMT PE=3 SV=1)

HSP 1 Score: 179.104 bits (453), Expect = 6.067e-58
Identity = 90/178 (50.56%), Postives = 115/178 (64.61%), Query Frame = -2

Query: 1561 ISRLRKANISWKTPMNFIVKQPY-----------FADDSDIL------KSDCLKNKNAFPPNFIHSLDSTHMMLTALSCWEKNITFVSVHDCFWTHACDVDEMNKFCREEFVKLHSQPILENLSDYLLEKYQFQ----INPNENDNGRKIAFNKLLKNIPKKGTFDIREVLDSTYFFS 2031
            IS +   N+ W TP+   V QPY            A  S+ +      K + +K KNAFPPNFIHSLDS+HMMLT+L C +K +TFVSVHDCFWTHAC V +MN+ CRE+FV LHSQPILE+LS ++  KY FQ    +N    D+  K   N++L  +P+KG FD+R VL+S YFFS
Sbjct: 1180 ISGVCGQNVEWVTPLGLPVVQPYNRSEVKHTMRTGARVSETMAMDMYEKPNVMKQKNAFPPNFIHSLDSSHMMLTSLYCEQKGLTFVSVHDCFWTHACSVPDMNQICREQFVSLHSQPILEDLSTFMRSKYTFQESDMLNDGSADDLSKRRLNRILGEMPRKGKFDLRNVLNSVYFFS 1357

HSP 2 Score: 77.411 bits (189), Expect = 6.067e-58
Identity = 55/105 (52.38%), Postives = 70/105 (66.67%), Query Frame = -3

Query: 2088 QDVYSDVLEHLEVKRKEDAKNGVEEAILLEGLLSRKVIKXXXXXXXXXXXXXXXTLQILRELKNLEKLPPQNIKSCAAYLSKLTLGSIGEIFASATNIQNWFIEV 2402
            QDVYS V   +E  RK +A NGV+ A  LEG + RKVIKQTVMTTVYGVT+YG  LQI R+LK++E  P + +   + YL+  T  S+ E+F S   IQ+WF E 
Sbjct: 1072 QDVYSAVAALVEKSRKSEALNGVQVAQALEGFVRRKVIKQTVMTTVYGVTRYGARLQIARQLKDIESFPKEWVWPASTYLTSKTFESLREMFTSTREIQDWFTEC 1176

[Close]

BLAST of SMED30023981 vs. Ensembl Cavefish
Match: polrmt (RNA polymerase mitochondrial [Source:NCBI gene;Acc:103025872])

HSP 1 Score: 169.859 bits (429), Expect = 2.387e-56
Identity = 85/171 (49.71%), Postives = 107/171 (62.57%), Query Frame = -2

Query: 1561 ANISWKTPMNFIVKQPYFADDSDILKS-----------------DCLKNKNAFPPNFIHSLDSTHMMLTALSCWEKNITFVSVHDCFWTHACDVDEMNKFCREEFVKLHSQPILENLSDYLLEKYQFQINPNENDNGRKIAFNK---LLKNIPKKGTFDIREVLDSTYFFS 2013
            + + W TP+   + QPY    S +++S                 D +K KNAFPPNFIHSLDSTHMMLTAL C+   +TFVSVHDCFWTH   VD MNK CRE+FV LH+QPIL+NLS++LL+KY          N   + FN+   LL  +P+ G FD++ V DSTYFFS
Sbjct: 1036 STVEWVTPLGLPIVQPYHRSRSQLVRSNIQMLNVQISYDVHEKPDTMKQKNAFPPNFIHSLDSTHMMLTALYCYSAGVTFVSVHDCFWTHPITVDTMNKVCREQFVSLHNQPILQNLSEFLLQKYCS--GSVGKTNKAFLEFNRMQQLLARVPETGDFDLQRVKDSTYFFS 1204

HSP 2 Score: 70.4774 bits (171), Expect = 2.387e-56
Identity = 50/104 (48.08%), Postives = 68/104 (65.38%), Query Frame = -3

Query: 2091 QDVYSDVLEHLEVKRKEDAKNGVEEAILLEGLLSRKVIKXXXXXXXXXXXXXXXTLQILRELKNLEKLPPQNIKSCAAYLSKLTLGSIGEIFASATNIQNWFIE 2402
            QDVYS V + +E  RK DA  G++ AI+LEG ++RKV+KQTVMT VYGVT+YGG LQI + LK ++  P + +   + YL +    S+ E+F     IQNW  E
Sbjct:  923 QDVYSGVAQQVEEFRKRDAAKGLKTAIVLEGFINRKVVKQTVMTVVYGVTRYGGRLQIEKRLKEIDDFPKEYLWDASHYLVQQVFSSLKEMFTGTREIQNWLTE 1026

[Close]

BLAST of SMED30023981 vs. Ensembl Cavefish
Match: polrmt (RNA polymerase mitochondrial [Source:NCBI gene;Acc:103025872])

HSP 1 Score: 169.474 bits (428), Expect = 3.551e-56
Identity = 85/171 (49.71%), Postives = 107/171 (62.57%), Query Frame = -2

Query: 1561 ANISWKTPMNFIVKQPYFADDSDILKS-----------------DCLKNKNAFPPNFIHSLDSTHMMLTALSCWEKNITFVSVHDCFWTHACDVDEMNKFCREEFVKLHSQPILENLSDYLLEKYQFQINPNENDNGRKIAFNK---LLKNIPKKGTFDIREVLDSTYFFS 2013
            + + W TP+   + QPY    S +++S                 D +K KNAFPPNFIHSLDSTHMMLTAL C+   +TFVSVHDCFWTH   VD MNK CRE+FV LH+QPIL+NLS++LL+KY          N   + FN+   LL  +P+ G FD++ V DSTYFFS
Sbjct: 1116 STVEWVTPLGLPIVQPYHRSRSQLVRSNIQMLNVQISYDVHEKPDTMKQKNAFPPNFIHSLDSTHMMLTALYCYSAGVTFVSVHDCFWTHPITVDTMNKVCREQFVSLHNQPILQNLSEFLLQKYCS--GSVGKTNKAFLEFNRMQQLLARVPETGDFDLQRVKDSTYFFS 1284

HSP 2 Score: 70.0922 bits (170), Expect = 3.551e-56
Identity = 50/104 (48.08%), Postives = 68/104 (65.38%), Query Frame = -3

Query: 2091 QDVYSDVLEHLEVKRKEDAKNGVEEAILLEGLLSRKVIKXXXXXXXXXXXXXXXTLQILRELKNLEKLPPQNIKSCAAYLSKLTLGSIGEIFASATNIQNWFIE 2402
            QDVYS V + +E  RK DA  G++ AI+LEG ++RKV+KQTVMT VYGVT+YGG LQI + LK ++  P + +   + YL +    S+ E+F     IQNW  E
Sbjct: 1003 QDVYSGVAQQVEEFRKRDAAKGLKTAIVLEGFINRKVVKQTVMTVVYGVTRYGGRLQIEKRLKEIDDFPKEYLWDASHYLVQQVFSSLKEMFTGTREIQNWLTE 1106

[Close]

BLAST of SMED30023981 vs. Ensembl Cavefish
Match: kansl3 (KAT8 regulatory NSL complex subunit 3 [Source:NCBI gene;Acc:103024162])

HSP 1 Score: 66.6254 bits (161), Expect = 8.402e-11
Identity = 69/318 (21.70%), Postives = 140/318 (44.03%), Query Frame = 2

Query:  518 QISKRIYYTYAASHWRMDICSTAHAYLLENLSDEYLVIYIESLRVMYHRKKVRTILDQLNIWAQNQKILHRFSWASLLEIIHREPEKLNEDLSEFSWNIENRWK--PIIIHIYS----DRWSDSNRLQKFIKILKTFSTVVDFPFDDS-----------------GRLRKKFEALRNQLQLELKHKKSSVFVMGWDLGALLGLLSSIKIHGYFTSVFCGVICLGLPLKGLKGERGIIPEDYIHLLTKETLIISGDMSSLGNGDDIEDFKDRMKGRVDIKHINIGGCDHLLRLSSGYAMRYRISQDVVDRLIAKNIRNF 1402
            + + ++ +  A+ +W   +    H+ ++E+LS   L  Y++ L+ +  + KV +++D++    Q+       S +  L ++ + P   +  +   S N  ++    P+I+   S       S S R++ +   L     V+  P +                   G +R K      ++   L HK   + ++GW++GAL      I  H         V+CLG PL+ + G RG + +  + + T    ++  +    G  +++E+F++++  R D   + +GG D  LR+SS       ++Q +VDR I   I +F
Sbjct:  173 KCAHKVRHALASVNWDTKLTQWLHSTMVESLSLTLLAAYLDVLQTL--KSKVPSLIDRM---LQSSSSKTGASGSEALSLLLKRP--WDPAVGVLSQNKPSKLPGSPLILICPSAPTLPSVSTSRRMRFWQSQLSCLGKVI--PINTHVGNGSNLGITQCLEHMVGTVRGKV----IEVHSHLPHKP--IILVGWNVGAL------IACHVSLMEYVTAVVCLGFPLQTVSGPRGDVDDPLLDMKTPVLFVVGQNALQCGM-ENMEEFREKI--RADNSMVVVGGADDNLRISSSKMKTEGLTQTMVDRCIQDEIVDF 466

[Close]

BLAST of SMED30023981 vs. Ensembl Cavefish
Match: kansl3 (KAT8 regulatory NSL complex subunit 3 [Source:NCBI gene;Acc:103024162])

HSP 1 Score: 66.2402 bits (160), Expect = 1.129e-10
Identity = 69/318 (21.70%), Postives = 140/318 (44.03%), Query Frame = 2

Query:  518 QISKRIYYTYAASHWRMDICSTAHAYLLENLSDEYLVIYIESLRVMYHRKKVRTILDQLNIWAQNQKILHRFSWASLLEIIHREPEKLNEDLSEFSWNIENRWK--PIIIHIYS----DRWSDSNRLQKFIKILKTFSTVVDFPFDDS-----------------GRLRKKFEALRNQLQLELKHKKSSVFVMGWDLGALLGLLSSIKIHGYFTSVFCGVICLGLPLKGLKGERGIIPEDYIHLLTKETLIISGDMSSLGNGDDIEDFKDRMKGRVDIKHINIGGCDHLLRLSSGYAMRYRISQDVVDRLIAKNIRNF 1402
            + + ++ +  A+ +W   +    H+ ++E+LS   L  Y++ L+ +  + KV +++D++    Q+       S +  L ++ + P   +  +   S N  ++    P+I+   S       S S R++ +   L     V+  P +                   G +R K      ++   L HK   + ++GW++GAL      I  H         V+CLG PL+ + G RG + +  + + T    ++  +    G  +++E+F++++  R D   + +GG D  LR+SS       ++Q +VDR I   I +F
Sbjct:  173 KCAHKVRHALASVNWDTKLTQWLHSTMVESLSLTLLAAYLDVLQTL--KSKVPSLIDRM---LQSSSSKTGASGSEALSLLLKRP--WDPAVGVLSQNKPSKLPGSPLILICPSAPTLPSVSTSRRMRFWQSQLSCLGKVI--PINTHVGNGSNLGITQCLEHMVGTVRGKV----IEVHSHLPHKP--IILVGWNVGAL------IACHVSLMEYVTAVVCLGFPLQTVSGPRGDVDDPLLDMKTPVLFVVGQNALQCGM-ENMEEFREKI--RADNSMVVVGGADDNLRISSSKMKTEGLTQTMVDRCIQDEIVDF 466

[Close]

BLAST of SMED30023981 vs. Ensembl Cavefish
Match: kansl3 (KAT8 regulatory NSL complex subunit 3 [Source:NCBI gene;Acc:103024162])

HSP 1 Score: 65.855 bits (159), Expect = 1.452e-10
Identity = 69/318 (21.70%), Postives = 140/318 (44.03%), Query Frame = 2

Query:  518 QISKRIYYTYAASHWRMDICSTAHAYLLENLSDEYLVIYIESLRVMYHRKKVRTILDQLNIWAQNQKILHRFSWASLLEIIHREPEKLNEDLSEFSWNIENRWK--PIIIHIYS----DRWSDSNRLQKFIKILKTFSTVVDFPFDDS-----------------GRLRKKFEALRNQLQLELKHKKSSVFVMGWDLGALLGLLSSIKIHGYFTSVFCGVICLGLPLKGLKGERGIIPEDYIHLLTKETLIISGDMSSLGNGDDIEDFKDRMKGRVDIKHINIGGCDHLLRLSSGYAMRYRISQDVVDRLIAKNIRNF 1402
            + + ++ +  A+ +W   +    H+ ++E+LS   L  Y++ L+ +  + KV +++D++    Q+       S +  L ++ + P   +  +   S N  ++    P+I+   S       S S R++ +   L     V+  P +                   G +R K      ++   L HK   + ++GW++GAL      I  H         V+CLG PL+ + G RG + +  + + T    ++  +    G  +++E+F++++  R D   + +GG D  LR+SS       ++Q +VDR I   I +F
Sbjct:  173 KCAHKVRHALASVNWDTKLTQWLHSTMVESLSLTLLAAYLDVLQTL--KSKVPSLIDRM---LQSSSSKTGASGSEALSLLLKRP--WDPAVGVLSQNKPSKLPGSPLILICPSAPTLPSVSTSRRMRFWQSQLSCLGKVI--PINTHVGNGSNLGITQCLEHMVGTVRGKV----IEVHSHLPHKP--IILVGWNVGAL------IACHVSLMEYVTAVVCLGFPLQTVSGPRGDVDDPLLDMKTPVLFVVGQNALQCGM-ENMEEFREKI--RADNSMVVVGGADDNLRISSSKMKTEGLTQTMVDRCIQDEIVDF 466

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BLAST of SMED30023981 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000005603.1 (pep scaffold:Pmarinus_7.0:GL476532:352361:404828:1 gene:ENSPMAG00000005070.1 transcript:ENSPMAT00000005603.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 70.4774 bits (171), Expect = 2.693e-13
Identity = 49/104 (47.12%), Postives = 68/104 (65.38%), Query Frame = -3

Query: 2091 QDVYSDVLEHLEVKRKEDAKNGVEEAILLEGLLSRKVIKXXXXXXXXXXXXXXXTLQILRELKNLEKLPPQNIKSCAAYLSKLTLGSIGEIFASATNIQNWFIE 2402
            QDVY+ V + ++V R EDAKN V+ A +L G + RK++KQTVMT VYGVT+YGG  QI + L  ++  P + +   +AYL+KL   S+ E+F     IQNW  E
Sbjct:  746 QDVYASVAQQVDVFRAEDAKNDVKIAQVLGGFIGRKLVKQTVMTVVYGVTRYGGRQQIEKRLSEMDDFPKEYVWDASAYLTKLVFSSLREMFTGTREIQNWLTE 849

HSP 2 Score: 22.3274 bits (46), Expect = 2.693e-13
Identity = 6/16 (37.50%), Postives = 10/16 (62.50%), Query Frame = -2

Query: 1963 NISWKTPMNFIVKQPY 2010
            ++ W TP+   + QPY
Sbjct:  860 SVEWVTPLGLPIIQPY 875

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BLAST of SMED30023981 vs. Ensembl Sea Lamprey
Match: kansl3 (KAT8 regulatory NSL complex subunit 3 [Source:NCBI gene;Acc:555951])

HSP 1 Score: 65.855 bits (159), Expect = 1.224e-11
Identity = 78/352 (22.16%), Postives = 155/352 (44.03%), Query Frame = 2

Query:  389 WTISDTNMKTRLLFILEEGYGSLIVLPTINENLDLINFNNSFRQISKRIYYTYAASHWRMDICSTAHAYLLENLSDEYLVIYIESLRVMYHRKKVRTILDQLNIWAQNQKILHRFSWASLLEIIHREP-EKLNEDLSEFSWNIENRWKPIIIHIYSDR---------------W-SDSNRLQKFIKI-LKTFSTVVDFPFDDSGRLRKKFEALRNQ---LQLELKHKKSSVFVMGWDLGALLGLLSSIKIHGYFTSVFCGVICLGLPLKGLKGERGIIPEDYIHLLTKETLIISGDMSSLGNGDDIEDFKDRMKGRVDIKHINIGGCDHLLRLSSGYAMRYRISQDVVDRLI 1381
            W+IS   + T+++  L+    + +    ++   + +    +  + + R+    A+  W   +    HA L+++LS   L  Y++ L+ +  R KV +++D++ I     K     + A  L ++ + P +     LS++   ++    P+++   S                 W S  + L K + + + T S    F       L     A+R +   LQL+       V ++GW++GAL      +  H         V+CLG PL   +GERG + +D +  L   TL + G  S+L +   +++F++R++    +  + +GG D  LR++        ++Q +VDR I
Sbjct:   62 WSISQNKLFTKVVKALQADRLTRLAYQAVSG--EAVLRRAAVDRCASRVRAALASCGWEPRLTQWLHATLIDSLSVAALSSYLDVLQTL--RSKVPSLVDRM-IAPSTTK--SEATSAEALSLLLKRPWDPAVGLLSQYK-PVKLLGAPLLLVAPSGPTASTAAAAASRRLRFWNSQLSCLGKVVPVTMHTTSGGAGFSVGQC--LEHMVGAVRTKVVCLQLQHHFPNRPVVLIGWNVGAL------VACHVSLMESVSAVVCLGFPLFTTEGERGDV-DDPLLELRVPTLFVIGQRSTLSSPAAMDEFRERLRAHSGL--VIVGGADSSLRMNKTKRRLEGLTQSMVDRCI 394

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BLAST of SMED30023981 vs. Ensembl Yeast
Match: RPO41 (Mitochondrial RNA polymerase; single subunit enzyme similar to those of T3 and T7 bacteriophages; requires a specificity subunit encoded by MTF1 for promoter recognition; Mtf1p interacts with and stabilizes the Rpo41p-promoter complex, enhancing DNA bending and melting to facilitate pre-initiation open complex formation; Rpo41p also synthesizes RNA primers for mitochondrial DNA replication [Source:SGD;Acc:S000001858])

HSP 1 Score: 98.2117 bits (243), Expect = 8.150e-27
Identity = 42/125 (33.60%), Postives = 70/125 (56.00%), Query Frame = -2

Query: 1687 ANISWKTPMNFIVKQPYFADDSDILKSD----------------CLKNKNAFPPNFIHSLDSTHMMLTALSCWEKNITFVSVHDCFWTHACDVDEMNKFCREEFVKLHSQPILENLSDYLLEKYQ 2013
            +++ W TP+   + QPY  +    ++++                  + K   PPNFIHSLD++HM+L+A  C ++ + F SVHD +WTHA D+D MN   RE+F+KLH   ++  L +   ++Y+
Sbjct: 1106 SSVIWTTPLGLPIVQPYREESKKQVETNLQTVFISDPFAVNPVNARRQKAGLPPNFIHSLDASHMLLSAAECGKQGLDFASVHDSYWTHASDIDTMNVVLREQFIKLHEVDLVLRLKEEFDQRYK 1230

HSP 2 Score: 40.0466 bits (92), Expect = 8.150e-27
Identity = 41/104 (39.42%), Postives = 63/104 (60.58%), Query Frame = -3

Query: 2091 QDVYSDVLEHLEVKRKEDAKNGVEEAILLEGLLSRKVIKXXXXXXXXXXXXXXXTLQILRELKNLEKLPPQNIKSCAAYLSKLTLGSIGEIFASATNIQNWFIE 2402
            QDVY+ V   ++ + +  A+ G E A +L+  ++RKV+KQTVMT VYGVT  G T QI ++L  +     +++   + YL+K    +I E+F SA  IQ+W  E
Sbjct:  976 QDVYAHVARLVQKRLEIAAEKGDENAKILKDKITRKVVKQTVMTNVYGVTYVGATFQIAKQLSPIFDDRKESL-DFSKYLTKHVFSAIRELFHSAHLIQDWLGE 1078

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BLAST of SMED30023981 vs. Ensembl Nematostella
Match: EDO47752 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RKW2])

HSP 1 Score: 140.969 bits (354), Expect = 1.234e-40
Identity = 72/167 (43.11%), Postives = 96/167 (57.49%), Query Frame = -2

Query: 1564 ISWKTPMNFIVKQPYFADDSDILKS-----------------DCLKNKNAFPPNFIHSLDSTHMMLTALSCWEKNITFVSVHDCFWTHACDVDEMNKFCREEFVKLHSQPILENLSDYLLEKYQFQ--INPNENDNGRKIAFNKLLKNIPKKGTFDIREVLDSTYFF 2007
            + W TP++  V QPY       +K+                 D  K + AFPPNF+HSLDSTHMMLTA+ C    I F SVHD FWTHA  +D MN+ CRE+FV LH+QPIL++L  + ++ Y     + P +  +   I  N   + +P+ G FD+ EVLDSTYFF
Sbjct:  520 VDWYTPLSLYVCQPYCKKAVKTIKTRLQGIEIPTQGFAMSVPDSRKQRAAFPPNFVHSLDSTHMMLTAVHCQRAGINFASVHDSFWTHAAHIDIMNRLCREQFVSLHTQPILQDLQTHFVDNYGGLKFMRPLKKKDHAPI-HNATFEGLPEIGDFDVSEVLDSTYFF 685

HSP 2 Score: 45.0542 bits (105), Expect = 1.234e-40
Identity = 40/81 (49.38%), Postives = 53/81 (65.43%), Query Frame = -3

Query: 2097 GVEEAILLEGLLSRKVIKXXXXXXXXXXXXXXXTLQILRELKNLEKLPPQNIKSCAAYLSKLTLGSIGEIFASATNIQNWF 2339
            G   A LLE  ++R+V+KQTVMT VYGVT  GG LQI R+LK+L+ L  + +   +AYL      SIG++F  A  IQ+WF
Sbjct:  427 GHRIATLLEDKVTRRVVKQTVMTIVYGVTFVGGRLQIERQLKDLD-LDERVLFEASAYLVHKVFASIGQMFTRAKAIQDWF 506

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BLAST of SMED30023981 vs. Ensembl Nematostella
Match: EDO28665 (DNA-directed RNA polymerase [Source:UniProtKB/TrEMBL;Acc:A7T5U5])

HSP 1 Score: 120.939 bits (302), Expect = 2.991e-39
Identity = 56/123 (45.53%), Postives = 73/123 (59.35%), Query Frame = -2

Query: 1690 ISWKTPMNFIVKQPYFADDSDILKS-----------------DCLKNKNAFPPNFIHSLDSTHMMLTALSCWEKNITFVSVHDCFWTHACDVDEMNKFCREEFVKLHSQPILENLSDYLLEKY 2007
            + W TP++  V QPY       +K+                 D  K + AFPPNF+HSLDSTHMMLTA+ C    I F SVHD FWTHA  +D MN+ CRE+FV LH+QPIL++L  + ++ Y
Sbjct:  291 VDWYTPLSLYVCQPYCKKAVKTIKTRLQGIEIPTQGFAMSVPDSRKQRAAFPPNFVHSLDSTHMMLTAVHCQRAGINFASVHDSFWTHAAHIDIMNRLCREQFVSLHTQPILQDLQAHFVDNY 413

HSP 2 Score: 60.4622 bits (145), Expect = 2.991e-39
Identity = 49/102 (48.04%), Postives = 67/102 (65.69%), Query Frame = -3

Query: 2097 QDVYSDVLEHLEVKRKEDAKNGVEEAILLEGLLSRKVIKXXXXXXXXXXXXXXXTLQILRELKNLEKLPPQNIKSCAAYLSKLTLGSIGEIFASATNIQNWF 2402
            QDVY++V + +E  R  DA+ G   A LLE  ++R+V+KQTVMT VYGVT  GG LQI R+LK+L+ L  + +   +AYL      SIG++F  A  IQ+WF
Sbjct:  177 QDVYAEVAKLVEEARLHDAEQGHRIATLLEDKVTRRVVKQTVMTIVYGVTFVGGRLQIERQLKDLD-LDERVLFEASAYLVHKVFASIGQMFTRAKAIQDWF 277

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BLAST of SMED30023981 vs. Ensembl Nematostella
Match: EDO41702 (DNA-directed RNA polymerase [Source:UniProtKB/TrEMBL;Acc:A7S3D0])

HSP 1 Score: 60.077 bits (144), Expect = 1.793e-19
Identity = 49/102 (48.04%), Postives = 67/102 (65.69%), Query Frame = -3

Query: 2097 QDVYSDVLEHLEVKRKEDAKNGVEEAILLEGLLSRKVIKXXXXXXXXXXXXXXXTLQILRELKNLEKLPPQNIKSCAAYLSKLTLGSIGEIFASATNIQNWF 2402
            QDVY++V + +E  R  DA+ G   A LLE  ++R+V+KQTVMT VYGVT  GG LQI R+LK+L+ L  + +   +AYL      SIG++F  A  IQ+WF
Sbjct:  313 QDVYAEVAKLVEEARLHDAEQGHRIATLLEDKVTRRVVKQTVMTIVYGVTFVGGRLQIERQLKDLD-LDERVLFEASAYLVHKVFASIGQMFTRAKAIQDWF 413

HSP 2 Score: 54.6842 bits (130), Expect = 1.793e-19
Identity = 28/69 (40.58%), Postives = 36/69 (52.17%), Query Frame = -2

Query: 1852 ISWKTPMNFIVKQPYFADDSDILKS-----------------DCLKNKNAFPPNFIHSLDSTHMMLTAL 2007
            + W TP++  V QPY       +K+                 D  K + AFPPNF+HSLDSTHMMLTA+
Sbjct:  427 VDWYTPLSLYVCQPYCKKAVKTIKTRLQGIEIPTQGFAMSVPDSRKQRAAFPPNFVHSLDSTHMMLTAV 495

[Close]

BLAST of SMED30023981 vs. Ensembl Nematostella
Match: EDO37729 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SEX0])

HSP 1 Score: 80.1073 bits (196), Expect = 2.883e-16
Identity = 53/167 (31.74%), Postives = 80/167 (47.90%), Query Frame = 2

Query:  944 GRLRKKFEALRNQLQLELKHKKSSVFVMGWDLGALLGLLSSIKIHGYFTSVFCGVICLGLPLKGLKGERGIIPEDYIHLLTKETLIISGDMSSLGNGDDIEDFKDRMKGRVDIKHINIGGCDHLLRLSSGYAMRYRISQDVVDRLIAKNIRNFAIDQVLHSYYKREE 1444
            G +R K   LR+            + ++GW +GAL      +          C V+CLG PL GL G RG I ED +  L   TL + G  S L   +DIE+ ++R+K    +  + +GG D  LRL+     +  ++Q++VDRLI   I  F +  VL S    ++
Sbjct:  147 GAVRTKVLELRSHF------PNRPIVLIGWSIGAL------VSCQVALMESVCAVVCLGFPLTGLDGVRGDI-EDPLLELKAPTLFVIGSNSCLNTQEDIEEVRERIKAETSL--LVVGGADEQLRLTRAKKKQEGLTQNMVDRLIMDQIGEF-LGNVLTSVNNNQQ 297

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BLAST of SMED30023981 vs. Ensembl Medaka
Match: polrmt (RNA polymerase mitochondrial [Source:NCBI gene;Acc:101162744])

HSP 1 Score: 171.785 bits (434), Expect = 2.730e-57
Identity = 86/171 (50.29%), Postives = 107/171 (62.57%), Query Frame = -2

Query: 1561 NISWKTPMNFIVKQPYFADDSDILKS-----------------DCLKNKNAFPPNFIHSLDSTHMMLTALSCWEKNITFVSVHDCFWTHACDVDEMNKFCREEFVKLHSQPILENLSDYLLEKY-QFQINPNENDNGRKIAFNK---LLKNIPKKGTFDIREVLDSTYFFS 2010
             + W TP+   + QPY    + +LKS                 D +K KNAFPPNFIHSLDSTHMMLTAL C+   +TFVSVHDCFWTHA  VD MNK CRE+FV LHSQPIL+ LSD+LL KY      P E  + +   + +   LL  +P+ G FD+++V +STYFFS
Sbjct: 1178 TVEWVTPLGLPIIQPYHRTRNQVLKSSIQHINLQISHDSKERPDTVKQKNAFPPNFIHSLDSTHMMLTALHCYSAGLTFVSVHDCFWTHALTVDTMNKVCREQFVALHSQPILQELSDFLLHKYCSGSGLPAEAKSKKYQEYRRLMLLLTKVPQTGDFDLQQVKESTYFFS 1348

HSP 2 Score: 71.633 bits (174), Expect = 2.730e-57
Identity = 50/104 (48.08%), Postives = 69/104 (66.35%), Query Frame = -3

Query: 2091 QDVYSDVLEHLEVKRKEDAKNGVEEAILLEGLLSRKVIKXXXXXXXXXXXXXXXTLQILRELKNLEKLPPQNIKSCAAYLSKLTLGSIGEIFASATNIQNWFIE 2402
            QDVYS V + +E  R  DA++G++ A +LEG +SRKV+KQTVMT VYGVT+YGG LQI + LK +E  P ++I   + YL +L    + E+F     IQ+W  E
Sbjct: 1064 QDVYSGVAQQVEELRARDARDGLKIAQVLEGFISRKVVKQTVMTVVYGVTRYGGRLQIEKRLKEIENFPKEHIWDASHYLVRLVFNGLKEMFTGTREIQDWLTE 1167

[Close]

BLAST of SMED30023981 vs. Ensembl Medaka
Match: kansl3 (KAT8 regulatory NSL complex subunit 3 [Source:NCBI gene;Acc:101169863])

HSP 1 Score: 64.3142 bits (155), Expect = 3.952e-10
Identity = 67/312 (21.47%), Postives = 136/312 (43.59%), Query Frame = 2

Query:  518 QISKRIYYTYAASHWRMDICSTAHAYLLENLSDEYLVIYIESLRVMYHRKKVRTILDQLNIW--------AQNQKILHRFSWASLLEIIHR-EPEKL-NEDLSEFSWNIENRWKPIIIHIYSDRW--SDSNRLQKFIKILKTFSTVVDFPFDDS-----GRLRKKFEALRNQLQLELKHKKSSVFVMGWDLGALLGLLSSIKIHGYFTSVFCGVICLGLPLKGLKGERGIIPEDYIHLLTKETLIISGDMSSLGNGDDIEDFKDRMKGRVDIKHINIGGCDHLLRLSSGYAMRYRISQDVVDRLIAKNIRNF 1402
            + ++++    A+ +W   +    H  L+E+LS   L  Y++ L+ +  + K+ +++D++ +         A+   +L +  W   + ++ + +P KL    L   + +  +   P +      R+  S  + L K I +    ++              G +R K      ++     HK   + ++GW+ GAL      I  H         V+CLG PL  + G RG + +  + + T    +I G  +   N + +E+F++++  R D   + +GG D  LR++S       ++Q +VDR I   I +F
Sbjct:  171 KCARKVRQALASMNWDTKLTQWLHTTLIESLSLPMLAAYLDVLQTL--KTKLPSLIDRMLLPTAKTGAPSAEALSLLLKRPWDPAVGVLSQNKPSKLPGSPLILIAPSTPS--NPALATTRRMRFWQSQLSCLGKVIPVHTHVNSGASISITQCLEHMLGTVRAKVM----EVHSHFPHKP--IVLVGWNAGAL------IACHVSLMEYLTAVVCLGFPLITVNGLRGDVDDPLLDMKTPVLFVI-GQHALQCNAESMEEFREKL--RADNSMVVVGGADDNLRINSAKMKSEGLTQTIVDRCIQDEIADF 463

[Close]

BLAST of SMED30023981 vs. Ensembl Medaka
Match: kansl3 (KAT8 regulatory NSL complex subunit 3 [Source:NCBI gene;Acc:101169863])

HSP 1 Score: 63.929 bits (154), Expect = 5.491e-10
Identity = 67/312 (21.47%), Postives = 135/312 (43.27%), Query Frame = 2

Query:  518 QISKRIYYTYAASHWRMDICSTAHAYLLENLSDEYLVIYIESLRVMYHRKKVRTILDQL--------NIWAQNQKILHRFSWASLLEIIHR-EPEKL-NEDLSEFSWNIENRWKPIIIHIYSDRW--SDSNRLQKFIKILKTFSTVVDFPFDDS-----GRLRKKFEALRNQLQLELKHKKSSVFVMGWDLGALLGLLSSIKIHGYFTSVFCGVICLGLPLKGLKGERGIIPEDYIHLLTKETLIISGDMSSLGNGDDIEDFKDRMKGRVDIKHINIGGCDHLLRLSSGYAMRYRISQDVVDRLIAKNIRNF 1402
            + ++++    A+ +W   +    H  L+E+LS   L  Y++ L+ +  + K+ +++D++           A+   +L +  W   + ++ + +P KL    L   + +  +   P +      R+  S  + L K I +    ++              G +R K      ++     HK   + ++GW+ GAL      I  H         V+CLG PL  + G RG + +  + + T    +I G  +   N + +E+F++++  R D   + +GG D  LR++S       ++Q +VDR I   I +F
Sbjct:  171 KCARKVRQALASMNWDTKLTQWLHTTLIESLSLPMLAAYLDVLQTL--KTKLPSLIDRMLLPTAKTGAPSAEALSLLLKRPWDPAVGVLSQNKPSKLPGSPLILIAPSTPS--NPALATTRRMRFWQSQLSCLGKVIPVHTHVNSGASISITQCLEHMLGTVRAKV----MEVHSHFPHKP--IVLVGWNAGAL------IACHVSLMEYLTAVVCLGFPLITVNGLRGDVDDPLLDMKTPVLFVI-GQHALQCNAESMEEFREKL--RADNSMVVVGGADDNLRINSAKMKSEGLTQTIVDRCIQDEIADF 463

[Close]

BLAST of SMED30023981 vs. Ensembl Medaka
Match: kansl3 (KAT8 regulatory NSL complex subunit 3 [Source:NCBI gene;Acc:101169863])

HSP 1 Score: 63.929 bits (154), Expect = 5.548e-10
Identity = 67/312 (21.47%), Postives = 135/312 (43.27%), Query Frame = 2

Query:  518 QISKRIYYTYAASHWRMDICSTAHAYLLENLSDEYLVIYIESLRVMYHRKKVRTILDQL--------NIWAQNQKILHRFSWASLLEIIHR-EPEKL-NEDLSEFSWNIENRWKPIIIHIYSDRW--SDSNRLQKFIKILKTFSTVVDFPFDDS-----GRLRKKFEALRNQLQLELKHKKSSVFVMGWDLGALLGLLSSIKIHGYFTSVFCGVICLGLPLKGLKGERGIIPEDYIHLLTKETLIISGDMSSLGNGDDIEDFKDRMKGRVDIKHINIGGCDHLLRLSSGYAMRYRISQDVVDRLIAKNIRNF 1402
            + ++++    A+ +W   +    H  L+E+LS   L  Y++ L+ +  + K+ +++D++           A+   +L +  W   + ++ + +P KL    L   + +  +   P +      R+  S  + L K I +    ++              G +R K      ++     HK   + ++GW+ GAL      I  H         V+CLG PL  + G RG + +  + + T    +I G  +   N + +E+F++++  R D   + +GG D  LR++S       ++Q +VDR I   I +F
Sbjct:  171 KCARKVRQALASMNWDTKLTQWLHTTLIESLSLPMLAAYLDVLQTL--KTKLPSLIDRMLLPTAKTGAPSAEALSLLLKRPWDPAVGVLSQNKPSKLPGSPLILIAPSTPS--NPALATTRRMRFWQSQLSCLGKVIPVHTHVNSGASISITQCLEHMLGTVRAKV----MEVHSHFPHKP--IVLVGWNAGAL------IACHVSLMEYLTAVVCLGFPLITVNGLRGDVDDPLLDMKTPVLFVI-GQHALQCNAESMEEFREKL--RADNSMVVVGGADDNLRINSAKMKSEGLTQTIVDRCIQDEIADF 463

[Close]

BLAST of SMED30023981 vs. Ensembl Medaka
Match: kansl3 (KAT8 regulatory NSL complex subunit 3 [Source:NCBI gene;Acc:101169863])

HSP 1 Score: 63.5438 bits (153), Expect = 6.633e-10
Identity = 67/312 (21.47%), Postives = 135/312 (43.27%), Query Frame = 2

Query:  518 QISKRIYYTYAASHWRMDICSTAHAYLLENLSDEYLVIYIESLRVMYHRKKVRTILDQL--------NIWAQNQKILHRFSWASLLEIIHR-EPEKL-NEDLSEFSWNIENRWKPIIIHIYSDRW--SDSNRLQKFIKILKTFSTVVDFPFDDS-----GRLRKKFEALRNQLQLELKHKKSSVFVMGWDLGALLGLLSSIKIHGYFTSVFCGVICLGLPLKGLKGERGIIPEDYIHLLTKETLIISGDMSSLGNGDDIEDFKDRMKGRVDIKHINIGGCDHLLRLSSGYAMRYRISQDVVDRLIAKNIRNF 1402
            + ++++    A+ +W   +    H  L+E+LS   L  Y++ L+ +  + K+ +++D++           A+   +L +  W   + ++ + +P KL    L   + +  +   P +      R+  S  + L K I +    ++              G +R K      ++     HK   + ++GW+ GAL      I  H         V+CLG PL  + G RG + +D +  +    L + G  +   N + +E+F++++  R D   + +GG D  LR++S       ++Q +VDR I   I +F
Sbjct:  171 KCARKVRQALASMNWDTKLTQWLHTTLIESLSLPMLAAYLDVLQTL--KTKLPSLIDRMLLPTAKTGAPSAEALSLLLKRPWDPAVGVLSQNKPSKLPGSPLILIAPSTPS--NPALATTRRMRFWQSQLSCLGKVIPVHTHVNSGASISITQCLEHMLGTVRAKVM----EVHSHFPHKP--IVLVGWNAGAL------IACHVSLMEYLTAVVCLGFPLITVNGLRGDV-DDPLLDMKTPVLFVIGQHALQCNAESMEEFREKL--RADNSMVVVGGADDNLRINSAKMKSEGLTQTIVDRCIQDEIADF 463

[Close]

BLAST of SMED30023981 vs. Planmine SMEST
Match: SMESG000026343.1 (SMESG000026343.1)

HSP 1 Score: 1005.74 bits (2599), Expect = 0.000e+0
Identity = 489/489 (100.00%), Postives = 489/489 (100.00%), Query Frame = 2

Query:   35 MLNWVDPYVSLNNIYHDHSYAFSESYYNNFVKNYYLQPSFHNPSEILLDNNANIEIGESDMIIDHNFIDNFSQLCGSVLKKLFGNKYEISSDNGIKTEISNTDSQDKSKCFNFFKSDEWTISDTNMKTRLLFILEEGYGSLIVLPTINENLDLINFNNSFRQISKRIYYTYAASHWRMDICSTAHAYLLENLSDEYLVIYIESLRVMYHRKKVRTILDQLNIWAQNQKILHRFSWASLLEIIHREPEKLNEDLSEFSWNIENRWKPIIIHIYSDRWSDSNRLQKFIKILKTFSTVVDFPFDDSGRLRKKFEALRNQLQLELKHKKSSVFVMGWDLGALLGLLSSIKIHGYFTSVFCGVICLGLPLKGLKGERGIIPEDYIHLLTKETLIISGDMSSLGNGDDIEDFKDRMKGRVDIKHINIGGCDHLLRLSSGYAMRYRISQDVVDRLIAKNIRNFAIDQVLHSYYKREELVEYTQTKGQSSTDQMKFF 1501
            MLNWVDPYVSLNNIYHDHSYAFSESYYNNFVKNYYLQPSFHNPSEILLDNNANIEIGESDMIIDHNFIDNFSQLCGSVLKKLFGNKYEISSDNGIKTEISNTDSQDKSKCFNFFKSDEWTISDTNMKTRLLFILEEGYGSLIVLPTINENLDLINFNNSFRQISKRIYYTYAASHWRMDICSTAHAYLLENLSDEYLVIYIESLRVMYHRKKVRTILDQLNIWAQNQKILHRFSWASLLEIIHREPEKLNEDLSEFSWNIENRWKPIIIHIYSDRWSDSNRLQKFIKILKTFSTVVDFPFDDSGRLRKKFEALRNQLQLELKHKKSSVFVMGWDLGALLGLLSSIKIHGYFTSVFCGVICLGLPLKGLKGERGIIPEDYIHLLTKETLIISGDMSSLGNGDDIEDFKDRMKGRVDIKHINIGGCDHLLRLSSGYAMRYRISQDVVDRLIAKNIRNFAIDQVLHSYYKREELVEYTQTKGQSSTDQMKFF
Sbjct:    1 MLNWVDPYVSLNNIYHDHSYAFSESYYNNFVKNYYLQPSFHNPSEILLDNNANIEIGESDMIIDHNFIDNFSQLCGSVLKKLFGNKYEISSDNGIKTEISNTDSQDKSKCFNFFKSDEWTISDTNMKTRLLFILEEGYGSLIVLPTINENLDLINFNNSFRQISKRIYYTYAASHWRMDICSTAHAYLLENLSDEYLVIYIESLRVMYHRKKVRTILDQLNIWAQNQKILHRFSWASLLEIIHREPEKLNEDLSEFSWNIENRWKPIIIHIYSDRWSDSNRLQKFIKILKTFSTVVDFPFDDSGRLRKKFEALRNQLQLELKHKKSSVFVMGWDLGALLGLLSSIKIHGYFTSVFCGVICLGLPLKGLKGERGIIPEDYIHLLTKETLIISGDMSSLGNGDDIEDFKDRMKGRVDIKHINIGGCDHLLRLSSGYAMRYRISQDVVDRLIAKNIRNFAIDQVLHSYYKREELVEYTQTKGQSSTDQMKFF 489

[Close]

BLAST of SMED30023981 vs. Planmine SMEST
Match: SMESG000026344.1 (SMESG000026344.1)

HSP 1 Score: 337.806 bits (865), Expect = 2.603e-137
Identity = 160/161 (99.38%), Postives = 160/161 (99.38%), Query Frame = -2

Query: 1561 LSSNISRLRKANISWKTPMNFIVKQPYFADDSDILKSDCLKNKNAFPPNFIHSLDSTHMMLTALSCWEKNITFVSVHDCFWTHACDVDEMNKFCREEFVKLHSQPILENLSDYLLEKYQFQINPNENDNGRKIAFNKLLKNIPKKGTFDIREVLDSTYFFS 2043
            LSSNISRLRKANISWKTPMNFIVKQPYFADDSDILKSDCLKNKNAFPPNFIHSLDSTHMMLTALSCWEKNITFVSVHDCFWTHACDVDEMNK CREEFVKLHSQPILENLSDYLLEKYQFQINPNENDNGRKIAFNKLLKNIPKKGTFDIREVLDSTYFFS
Sbjct:  896 LSSNISRLRKANISWKTPMNFIVKQPYFADDSDILKSDCLKNKNAFPPNFIHSLDSTHMMLTALSCWEKNITFVSVHDCFWTHACDVDEMNKICREEFVKLHSQPILENLSDYLLEKYQFQINPNENDNGRKIAFNKLLKNIPKKGTFDIREVLDSTYFFS 1056

HSP 2 Score: 172.17 bits (435), Expect = 2.603e-137
Identity = 104/105 (99.05%), Postives = 105/105 (100.00%), Query Frame = -3

Query: 2088 QDVYSDVLEHLEVKRKEDAKNGVEEAILLEGLLSRKVIKXXXXXXXXXXXXXXXTLQILRELKNLEKLPPQNIKSCAAYLSKLTLGSIGEIFASATNIQNWFIEV 2402
            QDVYSDVLEHLEVKRKEDAKNGVEEAILLEGLLSRKVIKQTVMTTVYGVTQYGGTLQILRELKNLEKLPPQNIKSCAAYLSKLTLGSIGEIFASATNIQNWFIE+
Sbjct:  792 QDVYSDVLEHLEVKRKEDAKNGVEEAILLEGLLSRKVIKQTVMTTVYGVTQYGGTLQILRELKNLEKLPPQNIKSCAAYLSKLTLGSIGEIFASATNIQNWFIEL 896

[Close]

BLAST of SMED30023981 vs. Planmine SMEST
Match: SMESG000026344.1 (SMESG000026344.1)

HSP 1 Score: 307.375 bits (786), Expect = 4.603e-128
Identity = 145/146 (99.32%), Postives = 145/146 (99.32%), Query Frame = -2

Query: 1606 LSSNISRLRKANISWKTPMNFIVKQPYFADDSDILKSDCLKNKNAFPPNFIHSLDSTHMMLTALSCWEKNITFVSVHDCFWTHACDVDEMNKFCREEFVKLHSQPILENLSDYLLEKYQFQINPNENDNGRKIAFNKLLKNIPKKG 2043
            LSSNISRLRKANISWKTPMNFIVKQPYFADDSDILKSDCLKNKNAFPPNFIHSLDSTHMMLTALSCWEKNITFVSVHDCFWTHACDVDEMNK CREEFVKLHSQPILENLSDYLLEKYQFQINPNENDNGRKIAFNKLLKNIPKKG
Sbjct:  896 LSSNISRLRKANISWKTPMNFIVKQPYFADDSDILKSDCLKNKNAFPPNFIHSLDSTHMMLTALSCWEKNITFVSVHDCFWTHACDVDEMNKICREEFVKLHSQPILENLSDYLLEKYQFQINPNENDNGRKIAFNKLLKNIPKKG 1041

HSP 2 Score: 171.785 bits (434), Expect = 4.603e-128
Identity = 104/105 (99.05%), Postives = 105/105 (100.00%), Query Frame = -3

Query: 2088 QDVYSDVLEHLEVKRKEDAKNGVEEAILLEGLLSRKVIKXXXXXXXXXXXXXXXTLQILRELKNLEKLPPQNIKSCAAYLSKLTLGSIGEIFASATNIQNWFIEV 2402
            QDVYSDVLEHLEVKRKEDAKNGVEEAILLEGLLSRKVIKQTVMTTVYGVTQYGGTLQILRELKNLEKLPPQNIKSCAAYLSKLTLGSIGEIFASATNIQNWFIE+
Sbjct:  792 QDVYSDVLEHLEVKRKEDAKNGVEEAILLEGLLSRKVIKQTVMTTVYGVTQYGGTLQILRELKNLEKLPPQNIKSCAAYLSKLTLGSIGEIFASATNIQNWFIEL 896

The following BLAST results are available for this feature:

BLAST of SMED30023981 vs. Ensembl Human
BLAST of SMED30023981 vs. Ensembl Celegans
BLAST of SMED30023981 vs. Ensembl Fly
BLAST of SMED30023981 vs. Ensembl Zebrafish
BLAST of SMED30023981 vs. Ensembl Xenopus
BLAST of SMED30023981 vs. Ensembl Mouse
BLAST of SMED30023981 vs. UniProt/SwissProt
BLAST of SMED30023981 vs. TrEMBL
BLAST of SMED30023981 vs. Ensembl Cavefish
BLAST of SMED30023981 vs. Ensembl Sea Lamprey
BLAST of SMED30023981 vs. Ensembl Yeast
BLAST of SMED30023981 vs. Ensembl Nematostella
BLAST of SMED30023981 vs. Ensembl Medaka
BLAST of SMED30023981 vs. Planmine SMEST

BLAST of SMED30023981 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5


Match Name	E-value	Identity	Description
POLRMT	1.786e-51	47.31	RNA polymerase mitochondrial [Source:HGNC Symbol;A...	[more]
POLRMT	5.179e-33	67.95	RNA polymerase mitochondrial [Source:HGNC Symbol;A...	[more]
KANSL3	7.043e-11	21.10	KAT8 regulatory NSL complex subunit 3 [Source:HGNC...	[more]
KANSL3	7.557e-11	21.41	KAT8 regulatory NSL complex subunit 3 [Source:HGNC...	[more]
KANSL3	8.521e-11	21.41	KAT8 regulatory NSL complex subunit 3 [Source:HGNC...	[more]

BLAST of SMED30023981 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 3


Match Name	E-value	Identity	Description
rpom-1	3.348e-42	44.59	DNA-directed RNA polymerase [Source:UniProtKB/TrE...	[more]
rpom-1	3.348e-42	44.59	DNA-directed RNA polymerase [Source:UniProtKB/TrE...	[more]
rpom-1	1.064e-39	44.59	DNA-directed RNA polymerase [Source:UniProtKB/TrE...	[more]

BLAST of SMED30023981 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 2


Match Name	E-value	Identity	Description
mtRNApol	1.631e-57	49.44	gene:FBgn0261938 transcript:FBtr0332273	[more]
mtRNApol	1.631e-57	49.44	gene:FBgn0261938 transcript:FBtr0077989	[more]

BLAST of SMED30023981 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 3


Match Name	E-value	Identity	Description
polrmt	3.988e-51	48.54	polymerase (RNA) mitochondrial (DNA directed) [Sou...	[more]
kansl3	4.514e-10	20.49	KAT8 regulatory NSL complex subunit 3 [Source:NCBI...	[more]
kansl3	5.128e-10	20.49	KAT8 regulatory NSL complex subunit 3 [Source:NCBI...	[more]

BLAST of SMED30023981 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5


Match Name	E-value	Identity	Description
pacrg	3.931e-53	48.84	parkin co-regulated [Source:Xenbase;Acc:XB-GENE-62...	[more]
pacrg	4.488e-53	48.84	parkin co-regulated [Source:Xenbase;Acc:XB-GENE-62...	[more]
pacrg	1.773e-49	49.09	parkin co-regulated [Source:Xenbase;Acc:XB-GENE-62...	[more]
pacrg	2.890e-49	48.47	parkin co-regulated [Source:Xenbase;Acc:XB-GENE-62...	[more]
pacrg	9.969e-47	54.84	parkin co-regulated [Source:Xenbase;Acc:XB-GENE-62...	[more]

BLAST of SMED30023981 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5


Match Name	E-value	Identity	Description
Polrmt	2.401e-52	45.56	polymerase (RNA) mitochondrial (DNA directed) [Sou...	[more]
Polrmt	2.766e-52	45.56	polymerase (RNA) mitochondrial (DNA directed) [Sou...	[more]
Polrmt	4.507e-49	45.56	polymerase (RNA) mitochondrial (DNA directed) [Sou...	[more]
Kansl3	1.829e-11	22.98	KAT8 regulatory NSL complex subunit 3 [Source:MGI ...	[more]
Kansl3	2.158e-11	22.98	KAT8 regulatory NSL complex subunit 3 [Source:MGI ...	[more]

BLAST of SMED30023981 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5


Match Name	E-value	Identity	Description
sp\|Q8BKF1\|RPOM_MOUSE	1.461e-51	45.56	DNA-directed RNA polymerase, mitochondrial OS=Mus ...	[more]
sp\|O00411\|RPOM_HUMAN	7.914e-51	47.31	DNA-directed RNA polymerase, mitochondrial OS=Homo...	[more]
sp\|O24600\|RPOT3_ARATH	1.618e-35	40.74	DNA-directed RNA polymerase 3, chloroplastic OS=Ar...	[more]
sp\|Q8L6J5\|RPO1B_TOBAC	1.862e-35	43.21	DNA-directed RNA polymerase 1B, mitochondrial OS=N...	[more]
sp\|Q93Y94\|RPOT1_NICSY	9.956e-35	42.59	DNA-directed RNA polymerase 1, mitochondrial OS=Ni...	[more]

BLAST of SMED30023981 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A034WBI1	7.922e-59	50.56	DNA-directed RNA polymerase (Fragment) OS=Bactroce...	[more]
A0A1A9UTY2	1.434e-58	52.02	DNA-directed RNA polymerase OS=Glossina austeni OX...	[more]
A0A0K8VPA8	2.627e-58	51.12	DNA-directed RNA polymerase OS=Bactrocera latifron...	[more]
A0A0P5SZN7	5.778e-58	50.62	DNA-directed RNA polymerase OS=Daphnia magna OX=35...	[more]
A0A0A1X790	6.067e-58	50.56	DNA-directed RNA polymerase OS=Zeugodacus cucurbit...	[more]

BLAST of SMED30023981 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
polrmt	2.387e-56	49.71	RNA polymerase mitochondrial [Source:NCBI gene;Acc...	[more]
polrmt	3.551e-56	49.71	RNA polymerase mitochondrial [Source:NCBI gene;Acc...	[more]
kansl3	8.402e-11	21.70	KAT8 regulatory NSL complex subunit 3 [Source:NCBI...	[more]
kansl3	1.129e-10	21.70	KAT8 regulatory NSL complex subunit 3 [Source:NCBI...	[more]
kansl3	1.452e-10	21.70	KAT8 regulatory NSL complex subunit 3 [Source:NCBI...	[more]

BLAST of SMED30023981 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 2


Match Name	E-value	Identity	Description
ENSPMAT00000005603.1	2.693e-13	47.12	pep scaffold:Pmarinus_7.0:GL476532:352361:404828:1...	[more]
kansl3	1.224e-11	22.16	KAT8 regulatory NSL complex subunit 3 [Source:NCBI...	[more]

BLAST of SMED30023981 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 1


Match Name	E-value	Identity	Description
RPO41	8.150e-27	33.60	Mitochondrial RNA polymerase; single subunit enzym...	[more]

BLAST of SMED30023981 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 4


Match Name	E-value	Identity	Description
EDO47752	1.234e-40	43.11	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO28665	2.991e-39	45.53	DNA-directed RNA polymerase [Source:UniProtKB/TrE...	[more]
EDO41702	1.793e-19	48.04	DNA-directed RNA polymerase [Source:UniProtKB/TrE...	[more]
EDO37729	2.883e-16	31.74	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]

BLAST of SMED30023981 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5


Match Name	E-value	Identity	Description
polrmt	2.730e-57	50.29	RNA polymerase mitochondrial [Source:NCBI gene;Acc...	[more]
kansl3	3.952e-10	21.47	KAT8 regulatory NSL complex subunit 3 [Source:NCBI...	[more]
kansl3	5.491e-10	21.47	KAT8 regulatory NSL complex subunit 3 [Source:NCBI...	[more]
kansl3	5.548e-10	21.47	KAT8 regulatory NSL complex subunit 3 [Source:NCBI...	[more]
kansl3	6.633e-10	21.47	KAT8 regulatory NSL complex subunit 3 [Source:NCBI...	[more]

BLAST of SMED30023981 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 3


Match Name	E-value	Identity	Description
SMESG000026343.1	0.000e+0	100.00	SMESG000026343.1	[more]
SMESG000026344.1	2.603e-137	99.38	SMESG000026344.1	[more]
SMESG000026344.1	4.603e-128	99.32	SMESG000026344.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence
protein sequence
protein sequence
protein sequence

transcript sequence

>SMED30023981 ID=SMED30023981|Name=SMED30023981|organism=Schmidtea mediterranea sexual|type=transcript|length=2404bp
TAATTATTATTTTTTCAATAAATCATAGTGCATTATGTTAAATTGGGTGG
ATCCATATGTATCATTAAACAATATTTATCACGACCACTCTTATGCATTT
AGTGAATCTTATTATAATAATTTTGTAAAGAATTATTATCTCCAGCCTTC
ATTTCACAATCCTTCAGAAATTTTATTAGATAATAATGCTAATATTGAAA
TAGGCGAAAGTGATATGATAATAGATCACAACTTTATTGATAATTTTAGT
CAACTTTGTGGGTCCGTATTAAAAAAACTATTTGGTAATAAATACGAAAT
TTCAAGTGACAATGGAATCAAAACCGAAATATCGAACACAGACTCTCAAG
ATAAGTCCAAGTGTTTCAATTTTTTTAAATCAGATGAATGGACTATTAGT
GACACAAATATGAAAACTCGATTGTTGTTCATATTGGAAGAAGGTTATGG
GTCTTTAATTGTTTTACCAACAATTAATGAAAATTTAGATCTTATTAATT
TTAACAACAGTTTTCGTCAAATTTCCAAACGAATTTATTACACATATGCC
GCATCACACTGGAGGATGGATATTTGTTCGACAGCTCACGCATATTTATT
GGAAAATTTATCTGATGAATATTTAGTCATTTATATTGAAAGTTTGCGCG
TTATGTATCATAGAAAAAAAGTCCGAACGATTCTTGATCAATTAAATATT
TGGGCTCAAAACCAAAAAATTCTTCATCGATTTTCCTGGGCCTCATTATT
AGAAATTATCCACCGAGAACCGGAAAAGCTGAATGAAGATTTATCGGAAT
TTAGCTGGAATATAGAGAATAGATGGAAACCGATAATAATTCATATTTAT
TCCGATAGGTGGTCGGACAGTAATCGATTGCAAAAATTTATAAAAATTCT
GAAAACTTTCTCAACAGTAGTTGACTTTCCATTTGATGATTCAGGTAGAC
TGAGGAAGAAATTTGAAGCGTTGCGCAATCAATTGCAATTAGAGCTAAAA
CACAAAAAATCTTCGGTATTTGTAATGGGCTGGGATTTGGGTGCATTGCT
GGGTTTGTTATCTTCCATTAAAATTCATGGTTACTTCACATCGGTTTTCT
GTGGCGTGATTTGTCTCGGGCTTCCGTTGAAGGGTCTCAAAGGTGAACGG
GGAATCATACCTGAGGATTATATACATTTATTAACAAAAGAAACCTTGAT
AATATCCGGTGACATGTCTAGTCTGGGAAATGGCGATGACATTGAAGATT
TTAAGGATCGAATGAAAGGTAGAGTCGATATTAAACACATAAATATTGGA
GGCTGCGATCATCTGTTACGACTGAGTTCAGGATATGCAATGAGATACAG
AATTTCACAGGATGTCGTTGATAGATTAATTGCTAAAAACATAAGAAATT
TTGCAATCGATCAAGTGTTGCATTCATATTACAAAAGAGAGGAACTAGTG
GAATATACACAAACAAAAGGACAGAGTTCTACTGATCAGATGAAATTTTT
CAATTAAATATGTAATAATATAATAAATGCTGAATTTTTATTTTTTCTAA
AAACATTTCAACTAAAAAAATAAGTCGAGTCCAAAACTTCTCTGATATCG
AAAGTTCCCTTTTTCGGTATATTTTTTAATAATTTATTAAATGCTATTTT
TCTGCCATTGTCATTTTCATTTGGATTAATCTGGAATTGATACTTTTCTA
AAAGATAATCTGATAAATTTTCAAGAATTGGTTGCGAATGTAATTTTACA
AATTCTTCTCTGCAAAACTTATTCATTTCATCAACATCGCAAGCATGTGT
CCAAAAACAATCGTGTACTGATACGAATGTTATGTTTTTTTCCCAGCAAC
TCAGAGCCGTCAACATCATGTGAGTCGAATCAAGGGAATGAATAAAGTTA
GGAGGAAATGCATTTTTATTTTTGAGACAATCTGATTTCAAAATGTCACT
ATCATCAGCAAAATATGGTTGTTTCACGATAAAATTCATTGGTGTTTTCC
AGGAAATATTGGCTTTGCGTAATCTACTGATGTTAGAAGACAACTAGCAA
ATTTTATAAATATTTTCTAATATTTAGCAACTATAAGCACCTCAATAAAC
CAATTTTGAATATTAGTTGCTGAAGCAAAAATTTCTCCGATTGAACCCAA
TGTTAATTTGGATAAATAAGCAGCACACGATTTGATATTTTGCGGTGGCA
GTTTTTCAAGATTTTTCAATTCGCGTAAAATCTGTAAAGTTCCTCCATAT
TGAGTTACACCGTATACAGTTGTCATAACCGTTTGTTTGATAACTTTTCG
AGATAATAATCCTTCCAATAAAATTGCTTCTTCAACACCATTTTTAGCAT
CTTCTTTTCGTTTCACTTCTAGATGTTCTAAAACATCTGAATAAACATCT
TGTG

protein sequence of SMED30023981-orf-1

>SMED30023981-orf-1 ID=SMED30023981-orf-1|Name=SMED30023981-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=491bp
MLNWVDPYVSLNNIYHDHSYAFSESYYNNFVKNYYLQPSFHNPSEILLDN
NANIEIGESDMIIDHNFIDNFSQLCGSVLKKLFGNKYEISSDNGIKTEIS
NTDSQDKSKCFNFFKSDEWTISDTNMKTRLLFILEEGYGSLIVLPTINEN
LDLINFNNSFRQISKRIYYTYAASHWRMDICSTAHAYLLENLSDEYLVIY
IESLRVMYHRKKVRTILDQLNIWAQNQKILHRFSWASLLEIIHREPEKLN
EDLSEFSWNIENRWKPIIIHIYSDRWSDSNRLQKFIKILKTFSTVVDFPF
DDSGRLRKKFEALRNQLQLELKHKKSSVFVMGWDLGALLGLLSSIKIHGY
FTSVFCGVICLGLPLKGLKGERGIIPEDYIHLLTKETLIISGDMSSLGNG
DDIEDFKDRMKGRVDIKHINIGGCDHLLRLSSGYAMRYRISQDVVDRLIA
KNIRNFAIDQVLHSYYKREELVEYTQTKGQSSTDQMKFFN*

protein sequence of SMED30023981-orf-2

>SMED30023981-orf-2 ID=SMED30023981-orf-2|Name=SMED30023981-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=112bp
QDVYSDVLEHLEVKRKEDAKNGVEEAILLEGLLSRKVIKQTVMTTVYGVT
QYGGTLQILRELKNLEKLPPQNIKSCAAYLSKLTLGSIGEIFASATNIQN
WFIEVLIVAKY*

protein sequence of SMED30023981-orf-3

>SMED30023981-orf-3 ID=SMED30023981-orf-3|Name=SMED30023981-orf-3|organism=Schmidtea mediterranea sexual|type=polypeptide|length=144bp
MNFIVKQPYFADDSDILKSDCLKNKNAFPPNFIHSLDSTHMMLTALSCWE
KNITFVSVHDCFWTHACDVDEMNKFCREEFVKLHSQPILENLSDYLLEKY
QFQINPNENDNGRKIAFNKLLKNIPKKGTFDIREVLDSTYFFS*

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0000026	gut
PLANA:0000429	neoblast
PLANA:0002109	X1 cell
PLANA:0002111	X2 cell

Vocabulary: **INTERPRO**
Term	Definition
IPR002092	DNA-dir_Rpol_phage-type
IPR029058	AB_hydrolase

Vocabulary: **molecular function**
Term	Definition
GO:0003677	DNA binding
GO:0003899	DNA-directed 5'-3' RNA polymerase activity
GO:0043995	histone acetyltransferase activity (H4-K5 specific)
GO:0043996	histone acetyltransferase activity (H4-K8 specific)
GO:0046972	histone acetyltransferase activity (H4-K16 specific)

Vocabulary: **biological process**
Term	Definition
GO:0006351	transcription, DNA-templated
GO:0016569	covalent chromatin modification
GO:0043981	histone H4-K5 acetylation
GO:0043982	histone H4-K8 acetylation
GO:0043984	histone H4-K16 acetylation

Vocabulary: **cellular component**
Term	Definition
GO:0000123	histone acetyltransferase complex
GO:0005634	nucleus
GO:0005654	nucleoplasm
GO:0005730	nucleolus
GO:0043231	intracellular membrane-bounded organelle

InterPro

Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002092	DNA-directed RNA polymerase, phage-type	PFAM	PF00940	RNA_pol	coord: 12..143 e-value: 1.5E-50 score: 172.2
IPR002092	DNA-directed RNA polymerase, phage-type	PANTHER	PTHR10102	DNA-DIRECTED RNA POLYMERASE, MITOCHONDRIAL	coord: 14..143
None	No IPR available	GENE3D	G3DSA:3.30.70.370		coord: 19..132 e-value: 4.3E-20 score: 74.2
None	No IPR available	PANTHER	PTHR10102:SF0	DNA-DIRECTED RNA POLYMERASE, MITOCHONDRIAL	coord: 14..143
None	No IPR available	SUPERFAMILY	SSF56672	DNA/RNA polymerases	coord: 5..143