Transcription factor glial cells missing

Overview
NameTranscription factor glial cells missing
Smed IDSMED30023953
Length (bp)1218
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Transcription factor glial cells missing (SMED30023953) t-SNE clustered cells

Violin plots show distribution of expression levels for Transcription factor glial cells missing (SMED30023953) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Transcription factor glial cells missing (SMED30023953) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Transcription factor glial cells missing (SMED30023953) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30023953

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 3

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
prepharyngeal regionSMED30023953h1SMcG0013982 SmedASXL_011737SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
X1 cellSMED30023953h1SMcG0013982 SmedASXL_011737SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
neoblastSMED30023953h1SMcG0013982 dd_Smed_v4_15107_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Transcription factor glial cells missing vs. Ensembl Human
Match: GCM1 (glial cells missing transcription factor 1 [Source:HGNC Symbol;Acc:HGNC:4197])

HSP 1 Score: 179.874 bits (455), Expect = 1.860e-51
Identity = 84/165 (50.91%), Postives = 115/165 (69.70%), Query Frame = 1 
Query:  124 INEWDVADLSLPQLIETND-FQIWPDGHIKLVYDYSKNERVRRHISGWAMRNTNNHNREILKKSCLGVLLCEKKCIRPGTREYVVLRPAICDKARKKQMKNKCPNCNGNLILQPCKGNLGFPVTHYWRRDEDRVFFQAKGIHDHLKPDLRPHRENRKKSSESNNV 615
            I  WD+ D+ LPQ ++  D FQ WPD + K +Y  S+++  +RH+S WAMRNTNNHN  ILKKSCLGV++C + C+    R+ + LRPAICDKAR+KQ + +CPNC+G L L PC+G+ GFPVT++WR D   +FFQ+KG HDH KP+ +   E R+   + N  
Sbjct:   13 ILSWDINDVKLPQNVKKTDWFQEWPDSYAKHIYS-SEDKNAQRHLSSWAMRNTNNHNSRILKKSCLGVVVCGRDCLAEEGRK-IYLRPAICDKARQKQQRKRCPNCDGPLKLIPCRGHGGFPVTNFWRHDGRFIFFQSKGEHDHPKPETKLEAEARRAMKKVNTA 175
[Close]
BLAST of Transcription factor glial cells missing vs. Ensembl Human
Match: GCM2 (glial cells missing transcription factor 2 [Source:HGNC Symbol;Acc:HGNC:4198])

HSP 1 Score: 179.874 bits (455), Expect = 6.649e-51
Identity = 82/157 (52.23%), Postives = 113/157 (71.97%), Query Frame = 1 
Query:  133 WDVADLSLPQLIETND-FQIWPDGHIKLVYDYSKNERVRRHISGWAMRNTNNHNREILKKSCLGVLLCEKKCIRP-GTREYVVLRPAICDKARKKQMKNKCPNCNGNLILQPCKGNLGFPVTHYWRRDEDRVFFQAKGIHDHLKPDLRPHRENRKKS 597
            WD+ D  +PQ +   D F+ WPDG+++ +Y  S  ++ +RH+SGWAMRNTNNHN  ILKKSCLGV++C + C  P G+R  + LRPAICDKAR KQ K  CPNC+  L L PC+G+ G+PVT++WR D + +FFQAKG+HDH +P+ +   E R+ +
Sbjct:   21 WDINDPQMPQELALFDQFREWPDGYVRFIYS-SDEKKAQRHLSGWAMRNTNNHNGHILKKSCLGVVVCTQACTLPDGSR--LQLRPAICDKARLKQQKKACPNCHSALELIPCRGHSGYPVTNFWRLDGNAIFFQAKGVHDHPRPESKSETEARRSA 174
[Close]
BLAST of Transcription factor glial cells missing vs. Ensembl Fly
Match: gcm (gene:FBgn0014179 transcript:FBtr0335492)

HSP 1 Score: 187.963 bits (476), Expect = 3.155e-54
Identity = 85/156 (54.49%), Postives = 109/156 (69.87%), Query Frame = 1 
Query:  130 EWDVADLSLPQLIETNDFQIWPDGHIKLVYDYSKNERVRRHISGWAMRNTNNHNREILKKSCLGVLLCEKKCIRPGTREYVVLRPAICDKARKKQMKNKCPN--CNGNLILQPCKGNLGFPVTHYWRRDEDRVFFQAKGIHDHLKPDLRPHRENRK 591
            +WD+ D  +P + E +DF  W +GH +L+Y    +E  R+H SGWAMRNTNNHN  ILKKSCLGVLLC  KC  P     V LRPAICDKAR+KQ   +CPN  CNG L +Q C+G+ G+PVTH+WRRD + ++FQAKG HDH +P+ +   E R+
Sbjct:   33 DWDINDSKMPSVGEFDDFNDWSNGHCRLIYSVQSDE-ARKHASGWAMRNTNNHNVNILKKSCLGVLLCSAKCKLPNGAS-VHLRPAICDKARRKQQGKQCPNRNCNGRLEIQACRGHCGYPVTHFWRRDGNGIYFQAKGTHDHPRPEAKGSTEARR 186
[Close]
BLAST of Transcription factor glial cells missing vs. Ensembl Fly
Match: gcm (gene:FBgn0014179 transcript:FBtr0079855)

HSP 1 Score: 187.963 bits (476), Expect = 3.155e-54
Identity = 85/156 (54.49%), Postives = 109/156 (69.87%), Query Frame = 1 
Query:  130 EWDVADLSLPQLIETNDFQIWPDGHIKLVYDYSKNERVRRHISGWAMRNTNNHNREILKKSCLGVLLCEKKCIRPGTREYVVLRPAICDKARKKQMKNKCPN--CNGNLILQPCKGNLGFPVTHYWRRDEDRVFFQAKGIHDHLKPDLRPHRENRK 591
            +WD+ D  +P + E +DF  W +GH +L+Y    +E  R+H SGWAMRNTNNHN  ILKKSCLGVLLC  KC  P     V LRPAICDKAR+KQ   +CPN  CNG L +Q C+G+ G+PVTH+WRRD + ++FQAKG HDH +P+ +   E R+
Sbjct:   33 DWDINDSKMPSVGEFDDFNDWSNGHCRLIYSVQSDE-ARKHASGWAMRNTNNHNVNILKKSCLGVLLCSAKCKLPNGAS-VHLRPAICDKARRKQQGKQCPNRNCNGRLEIQACRGHCGYPVTHFWRRDGNGIYFQAKGTHDHPRPEAKGSTEARR 186
[Close]
BLAST of Transcription factor glial cells missing vs. Ensembl Fly
Match: gcm2 (gene:FBgn0019809 transcript:FBtr0079837)

HSP 1 Score: 179.104 bits (453), Expect = 3.172e-50
Identity = 83/165 (50.30%), Postives = 112/165 (67.88%), Query Frame = 1 
Query:   85 SSINIDVTATTPLINEWDVADLSLPQL--IETNDFQIWPDGHIKLVYDYSKNERVRRHISGWAMRNTNNHNREILKKSCLGVLLCEKKCIRPGTREYVVLRPAICDKARKKQMKNKCPN--C-NGNLILQPCKGNLGFPVTHYWRRDEDRVFFQAKGIHDHLKPD 564
             S+ +  ++T     EWD+ D  +P +   E ++F  W DGH++ +Y    NE  ++HISGWAMRNTNNHN  ILKKSCLGVL+C + C  P   + + LRPAICDKAR+KQ    CPN  C  G L ++PC+G+ G+PVTH+WR   + +FFQAKG+HDHL+PD
Sbjct:   51 GSMTMPSSSTGKGKREWDINDAIVPHVPDQEFDEFNEWSDGHVRHIYSL-HNEEAKKHISGWAMRNTNNHNVNILKKSCLGVLVCSQHCTLPNGSK-INLRPAICDKARRKQEGKACPNKSCRGGRLEIKPCRGHCGYPVTHFWRHSGNAIFFQAKGVHDHLRPD 213
[Close]
BLAST of Transcription factor glial cells missing vs. Ensembl Zebrafish
Match: gcm2 (glial cells missing transcription factor 2 [Source:ZFIN;Acc:ZDB-GENE-050127-1])

HSP 1 Score: 179.104 bits (453), Expect = 5.679e-51
Identity = 82/156 (52.56%), Postives = 112/156 (71.79%), Query Frame = 1 
Query:  133 WDVADLSLPQLIETND-FQIWPDGHIKLVYDYSKNERVRRHISGWAMRNTNNHNREILKKSCLGVLLCEKKCIRPGTREYVVLRPAICDKARKKQMKNKCPNCNGNLILQPCKGNLGFPVTHYWRRDEDRVFFQAKGIHDHLKPDLRPHRENRKKS 597
            WD+ D  LPQ  +  D FQ W DG+++ +Y  S+++  +RH+SGWAMRNTNNHN +ILKKSCLGV++C + C  P   + + LRPAICDKAR+KQ K  CPNCN  L L PC+G+ G+PVT++WR D   +FFQAKG+HDH +P+ +   E R+ +
Sbjct:    5 WDINDPKLPQDTKQYDAFQEWTDGYVRYIYS-SEDKNAQRHLSGWAMRNTNNHNCQILKKSCLGVVVCSRNCSLPDGSK-LQLRPAICDKARQKQQKKLCPNCNSALELIPCRGHSGYPVTNFWRVDGKAIFFQAKGVHDHPRPESKSETEARRSA 158
[Close]
BLAST of Transcription factor glial cells missing vs. Ensembl Zebrafish
Match: gcm2 (glial cells missing transcription factor 2 [Source:ZFIN;Acc:ZDB-GENE-050127-1])

HSP 1 Score: 179.489 bits (454), Expect = 6.332e-51
Identity = 82/156 (52.56%), Postives = 112/156 (71.79%), Query Frame = 1 
Query:  133 WDVADLSLPQLIETND-FQIWPDGHIKLVYDYSKNERVRRHISGWAMRNTNNHNREILKKSCLGVLLCEKKCIRPGTREYVVLRPAICDKARKKQMKNKCPNCNGNLILQPCKGNLGFPVTHYWRRDEDRVFFQAKGIHDHLKPDLRPHRENRKKS 597
            WD+ D  LPQ  +  D FQ W DG+++ +Y  S+++  +RH+SGWAMRNTNNHN +ILKKSCLGV++C + C  P   + + LRPAICDKAR+KQ K  CPNCN  L L PC+G+ G+PVT++WR D   +FFQAKG+HDH +P+ +   E R+ +
Sbjct:   23 WDINDPKLPQDTKQYDAFQEWTDGYVRYIYS-SEDKNAQRHLSGWAMRNTNNHNCQILKKSCLGVVVCSRNCSLPDGSK-LQLRPAICDKARQKQQKKLCPNCNSALELIPCRGHSGYPVTNFWRVDGKAIFFQAKGVHDHPRPESKSETEARRSA 176
[Close]
BLAST of Transcription factor glial cells missing vs. Ensembl Xenopus
Match: gcm1 (glial cells missing transcription factor 1 [Source:NCBI gene;Acc:549669])

HSP 1 Score: 181.03 bits (458), Expect = 2.438e-52
Identity = 87/164 (53.05%), Postives = 111/164 (67.68%), Query Frame = 1 
Query:  133 WDVADLSLPQLIETND-FQIWPDGHIKLVYDYSKNERVRRHISGWAMRNTNNHNREILKKSCLGVLLCEKKCIRPGTREYVVLRPAICDKARKKQMKNKCPNCNGNLILQPCKGNLGFPVTHYWRRDEDRVFFQAKGIHDHLKPDLRPHRENRKKSSESNNVYM 621
            WD+ D+ LPQ  E  D FQ WPD ++K +Y  S N   +RH+SGWAMRNTNNHN  ILKKSCLGVL+C   C  P  R+ V LRPAICDKAR+KQ    CPNC+G L L  C+G+ GFPVT++WR +   ++FQ KG+HDH KP+ +   E+RK   +  N  +
Sbjct:   24 WDINDMKLPQDPEQTDWFQEWPDSYVKHIYS-SSNRNAQRHLSGWAMRNTNNHNSRILKKSCLGVLVCSNDCTVPDGRK-VYLRPAICDKARQKQQSKHCPNCSGPLKLISCRGHGGFPVTNFWRHEGPYIYFQTKGVHDHPKPETKLESESRKVGHKKRNAIV 185
[Close]
BLAST of Transcription factor glial cells missing vs. Ensembl Xenopus
Match: GCM2 (glial cells missing homolog 2 [Source:NCBI gene;Acc:100488293])

HSP 1 Score: 174.481 bits (441), Expect = 4.331e-49
Identity = 80/157 (50.96%), Postives = 115/157 (73.25%), Query Frame = 1 
Query:  133 WDVADLSLPQ-LIETNDFQIWPDGHIKLVYDYSKNERVRRHISGWAMRNTNNHNREILKKSCLGVLLCEKKC-IRPGTREYVVLRPAICDKARKKQMKNKCPNCNGNLILQPCKGNLGFPVTHYWRRDEDRVFFQAKGIHDHLKPDLRPHRENRKKS 597
            WD+ D  LPQ L + + FQ W DG+++ +Y+ ++++  +RH+SGWAMRNTNNHN +ILKKSCLGV++C + C +  G +  ++LRPAICDKAR+KQ K  C NCN  L L PC+G+ G+PVT++WR D   +FFQAKG+HDH +P+ +   E R+ +
Sbjct:   24 WDINDPKLPQDLKQFDSFQEWTDGYVRFIYN-AEDKNAQRHLSGWAMRNTNNHNCQILKKSCLGVVVCSRNCTLLDGGK--LLLRPAICDKARQKQQKKMCSNCNSALELIPCRGHSGYPVTNFWRLDGKAIFFQAKGVHDHPRPESKSETEARRSA 177
[Close]
BLAST of Transcription factor glial cells missing vs. Ensembl Xenopus
Match: gcm1 (glial cells missing transcription factor 1 [Source:NCBI gene;Acc:549669])

HSP 1 Score: 169.088 bits (427), Expect = 5.381e-48
Identity = 80/151 (52.98%), Postives = 104/151 (68.87%), Query Frame = 1 
Query:  169 ETNDFQIWPDGHIKLVYDYSKNERVRRHISGWAMRNTNNHNREILKKSCLGVLLCEKKCIRPGTREYVVLRPAICDKARKKQMKNKCPNCNGNLILQPCKGNLGFPVTHYWRRDEDRVFFQAKGIHDHLKPDLRPHRENRKKSSESNNVYM 621
            +T+ FQ WPD ++K +Y  S N   +RH+SGWAMRNTNNHN  ILKKSCLGVL+C   C  P  R+ V LRPAICDKAR+KQ    CPNC+G L L  C+G+ GFPVT++WR +   ++FQ KG+HDH KP+ +   E+RK   +  N  +
Sbjct:   16 QTDWFQEWPDSYVKHIYS-SSNRNAQRHLSGWAMRNTNNHNSRILKKSCLGVLVCSNDCTVPDGRK-VYLRPAICDKARQKQQSKHCPNCSGPLKLISCRGHGGFPVTNFWRHEGPYIYFQTKGVHDHPKPETKLESESRKVGHKKRNAIV 164
[Close]
BLAST of Transcription factor glial cells missing vs. Ensembl Mouse
Match: Gcm2 (glial cells missing homolog 2 [Source:MGI Symbol;Acc:MGI:1861438])

HSP 1 Score: 174.866 bits (442), Expect = 1.355e-52
Identity = 82/177 (46.33%), Postives = 122/177 (68.93%), Query Frame = 1 
Query:   73 MHLESSINIDVTATTPLINEWDVADLSLPQL-IETNDFQIWPDGHIKLVYDYSKNERVRRHISGWAMRNTNNHNREILKKSCLGVLLCEKKC-IRPGTREYVVLRPAICDKARKKQMKNKCPNCNGNLILQPCKGNLGFPVTHYWRRDEDRVFFQAKGIHDHLKPDLRPHRENRKKS 597
            M  +S+ + D   +  +   WD+ D  +PQ     + F+ WPDG+++ +Y  S+ ++ +RH+SGWAMRNTNNHN  ILKKSCLGV++C + C ++ G+  ++ LRPAICDKAR KQ K  CPNC+  L L PC+G+ G+PVT++WR D + +FFQAKG+HDH +P+ +   E R+ +
Sbjct:    1 MPADSTQDEDAVLSYGMKLTWDINDPQMPQEPTHFDHFREWPDGYVRFIYS-SQEKKAQRHLSGWAMRNTNNHNGHILKKSCLGVVVCARACALKDGS--HLQLRPAICDKARLKQQKKACPNCHSPLELVPCRGHSGYPVTNFWRLDGNAIFFQAKGVHDHPRPESKSETEGRRSA 174
[Close]
BLAST of Transcription factor glial cells missing vs. Ensembl Mouse
Match: Gcm1 (glial cells missing homolog 1 [Source:MGI Symbol;Acc:MGI:108045])

HSP 1 Score: 180.644 bits (457), Expect = 6.468e-52
Identity = 88/175 (50.29%), Postives = 119/175 (68.00%), Query Frame = 1 
Query:  124 INEWDVADLSLPQLIETND-FQIWPDGHIKLVYDYSKNERVRRHISGWAMRNTNNHNREILKKSCLGVLLCEKKCIRPGTREYVVLRPAICDKARKKQMKNKCPNCNGNLILQPCKGNLGFPVTHYWRRDEDRVFFQAKGIHDHLKPDLRPHRENRKK------SSESNNVYMEN 627
            I  WD+ D+ LPQ ++T D FQ WPD ++K +Y  S +   +RH+S WAMRNTNNHN  ILKKSCLGV++C + C     R+ + LRPAICDKAR+KQ +  CPNCNG L L PC+G+ GFPVT++WR D   +FFQ+KG HDH +P+ +   E R+       +S SN++ M+ 
Sbjct:   13 ILSWDINDVKLPQNVKTTDWFQEWPDSYVKHIYS-SDDRNAQRHLSSWAMRNTNNHNSRILKKSCLGVVVCSRDCSTEEGRK-IYLRPAICDKARQKQQRKSCPNCNGPLKLIPCRGHGGFPVTNFWRHDGRFIFFQSKGEHDHPRPETKLEAEARRAMKKVHMASASNSLRMKG 185
[Close]
BLAST of Transcription factor glial cells missing vs. Ensembl Mouse
Match: Gcm2 (glial cells missing homolog 2 [Source:MGI Symbol;Acc:MGI:1861438])

HSP 1 Score: 178.333 bits (451), Expect = 1.955e-50
Identity = 83/177 (46.89%), Postives = 122/177 (68.93%), Query Frame = 1 
Query:   73 MHLESSINIDVTATTPLINEWDVADLSLPQLIETND-FQIWPDGHIKLVYDYSKNERVRRHISGWAMRNTNNHNREILKKSCLGVLLCEKKC-IRPGTREYVVLRPAICDKARKKQMKNKCPNCNGNLILQPCKGNLGFPVTHYWRRDEDRVFFQAKGIHDHLKPDLRPHRENRKKS 597
            M  +S+ + D   +  +   WD+ D  +PQ     D F+ WPDG+++ +Y  S+ ++ +RH+SGWAMRNTNNHN  ILKKSCLGV++C + C ++ G+  ++ LRPAICDKAR KQ K  CPNC+  L L PC+G+ G+PVT++WR D + +FFQAKG+HDH +P+ +   E R+ +
Sbjct:    1 MPADSTQDEDAVLSYGMKLTWDINDPQMPQEPTHFDHFREWPDGYVRFIYS-SQEKKAQRHLSGWAMRNTNNHNGHILKKSCLGVVVCARACALKDGS--HLQLRPAICDKARLKQQKKACPNCHSPLELVPCRGHSGYPVTNFWRLDGNAIFFQAKGVHDHPRPESKSETEGRRSA 174
[Close]
BLAST of Transcription factor glial cells missing vs. UniProt/SwissProt
Match: sp|Q27403|GCM_DROME (Transcription factor glial cells missing OS=Drosophila melanogaster OX=7227 GN=gcm PE=1 SV=2)

HSP 1 Score: 187.963 bits (476), Expect = 3.162e-53
Identity = 85/156 (54.49%), Postives = 109/156 (69.87%), Query Frame = 1 
Query:  130 EWDVADLSLPQLIETNDFQIWPDGHIKLVYDYSKNERVRRHISGWAMRNTNNHNREILKKSCLGVLLCEKKCIRPGTREYVVLRPAICDKARKKQMKNKCPN--CNGNLILQPCKGNLGFPVTHYWRRDEDRVFFQAKGIHDHLKPDLRPHRENRK 591
            +WD+ D  +P + E +DF  W +GH +L+Y    +E  R+H SGWAMRNTNNHN  ILKKSCLGVLLC  KC  P     V LRPAICDKAR+KQ   +CPN  CNG L +Q C+G+ G+PVTH+WRRD + ++FQAKG HDH +P+ +   E R+
Sbjct:   33 DWDINDSKMPSVGEFDDFNDWSNGHCRLIYSVQSDE-ARKHASGWAMRNTNNHNVNILKKSCLGVLLCSAKCKLPNGAS-VHLRPAICDKARRKQQGKQCPNRNCNGRLEIQACRGHCGYPVTHFWRRDGNGIYFQAKGTHDHPRPEAKGSTEARR 186
[Close]
BLAST of Transcription factor glial cells missing vs. UniProt/SwissProt
Match: sp|P70348|GCM1_MOUSE (Chorion-specific transcription factor GCMa OS=Mus musculus OX=10090 GN=Gcm1 PE=1 SV=1)

HSP 1 Score: 180.644 bits (457), Expect = 4.524e-51
Identity = 88/175 (50.29%), Postives = 119/175 (68.00%), Query Frame = 1 
Query:  124 INEWDVADLSLPQLIETND-FQIWPDGHIKLVYDYSKNERVRRHISGWAMRNTNNHNREILKKSCLGVLLCEKKCIRPGTREYVVLRPAICDKARKKQMKNKCPNCNGNLILQPCKGNLGFPVTHYWRRDEDRVFFQAKGIHDHLKPDLRPHRENRKK------SSESNNVYMEN 627
            I  WD+ D+ LPQ ++T D FQ WPD ++K +Y  S +   +RH+S WAMRNTNNHN  ILKKSCLGV++C + C     R+ + LRPAICDKAR+KQ +  CPNCNG L L PC+G+ GFPVT++WR D   +FFQ+KG HDH +P+ +   E R+       +S SN++ M+ 
Sbjct:   13 ILSWDINDVKLPQNVKTTDWFQEWPDSYVKHIYS-SDDRNAQRHLSSWAMRNTNNHNSRILKKSCLGVVVCSRDCSTEEGRK-IYLRPAICDKARQKQQRKSCPNCNGPLKLIPCRGHGGFPVTNFWRHDGRFIFFQSKGEHDHPRPETKLEAEARRAMKKVHMASASNSLRMKG 185
[Close]
BLAST of Transcription factor glial cells missing vs. UniProt/SwissProt
Match: sp|Q9NP62|GCM1_HUMAN (Chorion-specific transcription factor GCMa OS=Homo sapiens OX=9606 GN=GCM1 PE=1 SV=1)

HSP 1 Score: 179.874 bits (455), Expect = 8.934e-51
Identity = 84/165 (50.91%), Postives = 115/165 (69.70%), Query Frame = 1 
Query:  124 INEWDVADLSLPQLIETND-FQIWPDGHIKLVYDYSKNERVRRHISGWAMRNTNNHNREILKKSCLGVLLCEKKCIRPGTREYVVLRPAICDKARKKQMKNKCPNCNGNLILQPCKGNLGFPVTHYWRRDEDRVFFQAKGIHDHLKPDLRPHRENRKKSSESNNV 615
            I  WD+ D+ LPQ ++  D FQ WPD + K +Y  S+++  +RH+S WAMRNTNNHN  ILKKSCLGV++C + C+    R+ + LRPAICDKAR+KQ + +CPNC+G L L PC+G+ GFPVT++WR D   +FFQ+KG HDH KP+ +   E R+   + N  
Sbjct:   13 ILSWDINDVKLPQNVKKTDWFQEWPDSYAKHIYS-SEDKNAQRHLSSWAMRNTNNHNSRILKKSCLGVVVCGRDCLAEEGRK-IYLRPAICDKARQKQQRKRCPNCDGPLKLIPCRGHGGFPVTNFWRHDGRFIFFQSKGEHDHPKPETKLEAEARRAMKKVNTA 175
[Close]
BLAST of Transcription factor glial cells missing vs. UniProt/SwissProt
Match: sp|O75603|GCM2_HUMAN (Chorion-specific transcription factor GCMb OS=Homo sapiens OX=9606 GN=GCM2 PE=1 SV=1)

HSP 1 Score: 179.874 bits (455), Expect = 3.193e-50
Identity = 82/157 (52.23%), Postives = 113/157 (71.97%), Query Frame = 1 
Query:  133 WDVADLSLPQLIETND-FQIWPDGHIKLVYDYSKNERVRRHISGWAMRNTNNHNREILKKSCLGVLLCEKKCIRP-GTREYVVLRPAICDKARKKQMKNKCPNCNGNLILQPCKGNLGFPVTHYWRRDEDRVFFQAKGIHDHLKPDLRPHRENRKKS 597
            WD+ D  +PQ +   D F+ WPDG+++ +Y  S  ++ +RH+SGWAMRNTNNHN  ILKKSCLGV++C + C  P G+R  + LRPAICDKAR KQ K  CPNC+  L L PC+G+ G+PVT++WR D + +FFQAKG+HDH +P+ +   E R+ +
Sbjct:   21 WDINDPQMPQELALFDQFREWPDGYVRFIYS-SDEKKAQRHLSGWAMRNTNNHNGHILKKSCLGVVVCTQACTLPDGSR--LQLRPAICDKARLKQQKKACPNCHSALELIPCRGHSGYPVTNFWRLDGNAIFFQAKGVHDHPRPESKSETEARRSA 174
[Close]
BLAST of Transcription factor glial cells missing vs. UniProt/SwissProt
Match: sp|Q9Z288|GCM1_RAT (Chorion-specific transcription factor GCMa OS=Rattus norvegicus OX=10116 GN=Gcm1 PE=2 SV=1)

HSP 1 Score: 177.948 bits (450), Expect = 4.763e-50
Identity = 83/157 (52.87%), Postives = 110/157 (70.06%), Query Frame = 1 
Query:  124 INEWDVADLSLPQLIETND-FQIWPDGHIKLVYDYSKNERVRRHISGWAMRNTNNHNREILKKSCLGVLLCEKKCIRPGTREYVVLRPAICDKARKKQMKNKCPNCNGNLILQPCKGNLGFPVTHYWRRDEDRVFFQAKGIHDHLKPDLRPHRENRK 591
            I  WD+ D+ LPQ ++  D FQ WPD ++K +Y  S +   +RH+S WAMRNTNNHN  ILKKSCLGV++C + C     R+ + LRPAICDKAR+KQ +  CPNCNG L L PC+G+ GFPVT++WR D   +FFQ+KG HDH +P+ +   E R+
Sbjct:   13 ILSWDINDMKLPQNVKKTDWFQEWPDSYVKHIYS-SDDRSAQRHLSSWAMRNTNNHNSRILKKSCLGVVVCSRDCSTEEGRK-IYLRPAICDKARQKQQRKSCPNCNGPLKLIPCRGHGGFPVTNFWRHDGRFIFFQSKGEHDHPRPETKLEAEARR 167
[Close]
BLAST of Transcription factor glial cells missing vs. TrEMBL
Match: A0A1B0FMT7 (GCM domain-containing protein OS=Glossina morsitans morsitans OX=37546 PE=4 SV=1)

HSP 1 Score: 192.586 bits (488), Expect = 3.007e-52
Identity = 92/181 (50.83%), Postives = 122/181 (67.40%), Query Frame = 1 
Query:   73 MHLESSINIDVTATTPLIN------EWDVADLSLPQLIETNDFQIWPDGHIKLVYDYSKNERVRRHISGWAMRNTNNHNREILKKSCLGVLLCEKKCIRPGTREYVVLRPAICDKARKKQMKNKCPN--CNGNLILQPCKGNLGFPVTHYWRRDEDRVFFQAKGIHDHLKPDLRPHRENRK 591
            M +++SIN+ +   +  ++      +WD+ D  +P + E +DFQ W +GH +LVY  + NE  R+H SGWAMRNTNNHN  ILKKSCLGVLLC  KC  P     V LRPAICDKAR+KQ    CPN  C+G L +QPC+G+ G+PVTH+WRR  + +FFQAKG HDH +P+ +   E R+
Sbjct:    1 MVVQTSINLPIRNNSSPVHISRVQIDWDINDSVVPHVTEYDDFQDWANGHCRLVYS-ANNEDARKHSSGWAMRNTNNHNINILKKSCLGVLLCSDKCKLPNGNN-VNLRPAICDKARRKQQGKPCPNRNCSGRLEIQPCRGHCGYPVTHFWRRSGNSIFFQAKGTHDHPRPEAKGSSEARR 179
[Close]
BLAST of Transcription factor glial cells missing vs. TrEMBL
Match: B3MUW7 (GCM domain-containing protein OS=Drosophila ananassae OX=7217 GN=Dana\GF21907 PE=4 SV=1)

HSP 1 Score: 191.815 bits (486), Expect = 3.108e-52
Identity = 88/164 (53.66%), Postives = 112/164 (68.29%), Query Frame = 1 
Query:  130 EWDVADLSLPQLIETNDFQIWPDGHIKLVYDYSKNERVRRHISGWAMRNTNNHNREILKKSCLGVLLCEKKCIRPGTREYVVLRPAICDKARKKQMKNKCPN--CNGNLILQPCKGNLGFPVTHYWRRDEDRVFFQAKGIHDHLKPDLRPHRENRKKSSESNNV 615
            +WD+ D  +P + E +DF  W +GH +L+Y    +E  R+H SGWAMRNTNNHN  ILKKSCLGVLLC  KC  P     V LRPAICDKAR+KQ   +CPN  CNG L +QPC+G+ G+PVTH+WRRD + ++FQAKG HDH +P+ +   E R+  S    V
Sbjct:   29 DWDINDAKMPTVGEFDDFSDWANGHCRLIYSAHSDE-ARKHASGWAMRNTNNHNVNILKKSCLGVLLCSAKCKLPNGAS-VHLRPAICDKARRKQQGKQCPNRNCNGRLEIQPCRGHCGYPVTHFWRRDGNGIYFQAKGTHDHPRPEAKGSTEARRLLSGGRRV 190
[Close]
BLAST of Transcription factor glial cells missing vs. TrEMBL
Match: A0A1W4VHS3 (transcription factor glial cells missing OS=Drosophila ficusphila OX=30025 GN=LOC108097825 PE=4 SV=1)

HSP 1 Score: 191.815 bits (486), Expect = 3.128e-52
Identity = 85/156 (54.49%), Postives = 109/156 (69.87%), Query Frame = 1 
Query:  130 EWDVADLSLPQLIETNDFQIWPDGHIKLVYDYSKNERVRRHISGWAMRNTNNHNREILKKSCLGVLLCEKKCIRPGTREYVVLRPAICDKARKKQMKNKCPN--CNGNLILQPCKGNLGFPVTHYWRRDEDRVFFQAKGIHDHLKPDLRPHRENRK 591
            +WD+ D  +P ++E +DF  W +GH +L+Y    +E  R+H SGWAMRNTNNHN  ILKKSCLGVLLC  KC  P     V LRPAICDKAR+KQ   +CPN  CNG L +Q C+G+ G+PVTH+WRRD   ++FQAKG HDH +P+ +   E R+
Sbjct:   33 DWDINDSKMPSVVEFDDFSDWANGHCRLIYSVQSDE-ARKHASGWAMRNTNNHNVNILKKSCLGVLLCSAKCKLPNGAS-VHLRPAICDKARRKQQGKQCPNRNCNGRLEIQACRGHCGYPVTHFWRRDGSGIYFQAKGTHDHPRPEAKGSTEARR 186
[Close]
BLAST of Transcription factor glial cells missing vs. TrEMBL
Match: A0A2R7W3I9 (Glial-cells-missing OS=Oncopeltus fasciatus OX=7536 GN=OFAS_OFAS006827 PE=4 SV=1)

HSP 1 Score: 182.185 bits (461), Expect = 6.141e-52
Identity = 86/165 (52.12%), Postives = 114/165 (69.09%), Query Frame = 1 
Query:  127 NEWDVADLSLPQLIETNDFQIWPDGHIKLVYDYSKNERVRRHISGWAMRNTNNHNREILKKSCLGVLLCEKKCIRPGTREYVVLRPAICDKARKKQMKNKCPN--CNGNLILQPCKGNLGFPVTHYWRRDEDRVFFQAKGIHDHLKPDLRPHRENRKKSSESNNV 615
            ++WD+ D ++P++ E N ++ W DGH +LVY    +E  +RH SGWAMRNTNNHN  ILKKSCLGVL+C ++C+ P   + V LRPAICDKARKKQ    CPN  CNG L + PC+G+ G+PVTH+WR  E  +FFQAKG+HDH +P+ +   E R+       V
Sbjct:   13 HDWDINDSNVPRVSEYNRWEEWADGHCRLVYP-PHSEEAKRHASGWAMRNTNNHNVHILKKSCLGVLVCSRRCVLPNG-DSVHLRPAICDKARKKQQGKPCPNRLCNGRLEILPCRGHCGYPVTHFWRHTEHAIFFQAKGVHDHQRPEAKSTSEARRSLGAGRRV 175
[Close]
BLAST of Transcription factor glial cells missing vs. TrEMBL
Match: A0A482WKB2 (GCM domain-containing protein OS=Laodelphax striatellus OX=195883 GN=LSTR_LSTR010437 PE=4 SV=1)

HSP 1 Score: 192.586 bits (488), Expect = 6.560e-52
Identity = 90/164 (54.88%), Postives = 115/164 (70.12%), Query Frame = 1 
Query:  130 EWDVADLSLPQLIETNDFQIWPDGHIKLVYDYSKNERVRRHISGWAMRNTNNHNREILKKSCLGVLLCEKKCIRPGTREYVVLRPAICDKARKKQMKNKCPN--CNGNLILQPCKGNLGFPVTHYWRRDEDRVFFQAKGIHDHLKPDLRPHRENRKKSSESNNV 615
            +WD+ D ++P++ E NDF+ W DGH +LVY  S +E  +RH SGWAMRNTNNHN  ILKKSCLGVL+C + C+ P   + V LRPAICDKARKKQ    CPN  CNG L + PC+G+ G+PVTH+WR  E  +FFQAKG+HDHL+P+ +   E R+       V
Sbjct:   29 DWDINDSNVPRVNEFNDFEEWADGHCRLVYPAS-SEEAKRHASGWAMRNTNNHNVHILKKSCLGVLVCSRHCLLPNG-DSVHLRPAICDKARKKQQGKPCPNRQCNGRLEVLPCRGHCGYPVTHFWRHTEHAIFFQAKGVHDHLRPEAKSASEARRSLGAGRRV 190
[Close]
BLAST of Transcription factor glial cells missing vs. Ensembl Cavefish
Match: gcm2 (glial cells missing transcription factor 2 [Source:NCBI gene;Acc:103026873])

HSP 1 Score: 175.252 bits (443), Expect = 1.830e-49
Identity = 81/157 (51.59%), Postives = 114/157 (72.61%), Query Frame = 1 
Query:  133 WDVADLSLPQLIETND-FQIWPDGHIKLVYDYSKNERVRRHISGWAMRNTNNHNREILKKSCLGVLLCEKKC-IRPGTREYVVLRPAICDKARKKQMKNKCPNCNGNLILQPCKGNLGFPVTHYWRRDEDRVFFQAKGIHDHLKPDLRPHRENRKKS 597
            WD+ D  LPQ  +  D FQ W DG+++ +Y  ++++  +RH+SGWAMRNTNNHN +ILKKSCLGV++C + C +  GT+  + LRPAICDKAR+KQ K  CPNC+  L L PC+G+ G+PVT++WR D   +FFQAKG+HDH +P+ +   E R+ +
Sbjct:   23 WDINDPKLPQDPKQFDPFQEWTDGYVRYIYS-AEDKNAQRHLSGWAMRNTNNHNCQILKKSCLGVVVCARGCTLADGTK--LQLRPAICDKARQKQQKKLCPNCSSALELVPCRGHSGYPVTNFWRVDGKAIFFQAKGVHDHPRPESKSETEARRSA 176
[Close]
BLAST of Transcription factor glial cells missing vs. Ensembl Sea Lamprey
Match: gcm2 (glial cells missing transcription factor 2 [Source:ZFIN;Acc:ZDB-GENE-050127-1])

HSP 1 Score: 173.711 bits (439), Expect = 1.522e-49
Identity = 83/158 (52.53%), Postives = 111/158 (70.25%), Query Frame = 1 
Query:  133 WDVADLSLPQ-LIETNDFQIWPDGHIKLVYDYSKNERVRRHISGWAMRNTNNHNREILKKSCLGVLLCEKKCIRPGTREYVVLRPAICDKARKKQMKNKCPNCNGNLILQPCKGNLGFPVTHYWRRDEDRVFFQAKGIHDHLKPDLRPHRENRKKSSE 603
            WD+ D  LPQ L + + FQ W DG+ + V+  ++++  +RH+SGWAMRNTNNHN  ILKKSCLGV+ C + C  P  R+ V LRPAICDKAR+KQ K  CPNC   L L PC+G+ G+PVT++WR +   +FFQAKG HDH +P+ +   E R+ SS+
Sbjct:   23 WDINDPKLPQDLKQVDPFQEWNDGYARFVFS-AEDKNAQRHLSGWAMRNTNNHNCAILKKSCLGVVACGRACTMPDGRK-VHLRPAICDKARQKQQKKLCPNCGSPLDLLPCRGHSGYPVTNFWRHEGRFIFFQAKGSHDHPRPESKTEAEARRSSSK 178
[Close]
BLAST of Transcription factor glial cells missing vs. Ensembl Sea Lamprey
Match: ENSPMAT00000001147.1 (pep scaffold:Pmarinus_7.0:GL476699:2797:4762:1 gene:ENSPMAG00000001027.1 transcript:ENSPMAT00000001147.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 72.0182 bits (175), Expect = 2.335e-16
Identity = 29/55 (52.73%), Postives = 40/55 (72.73%), Query Frame = 1 
Query:  427 CPNCNGNLILQPCKGNLGFPVTHYWRRDEDRVFFQAKGIHDHLKPDLRPHRENRK 591
            CPNC G L L PC+G+ G+PVT++WR +   VFFQAKG+HDH +P+ +   E R+
Sbjct:    3 CPNCQGPLELVPCRGHSGYPVTNFWRHEGKLVFFQAKGVHDHPRPESKTEAEGRR 57
[Close]
BLAST of Transcription factor glial cells missing vs. Ensembl Nematostella
Match: EDO33215 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SSU8])

HSP 1 Score: 139.428 bits (350), Expect = 4.887e-40
Identity = 68/144 (47.22%), Postives = 94/144 (65.28%), Query Frame = 1 
Query:  148 LSLPQLI-ETNDFQIWPDGHIKLVYDYSKNERVRRHISGWAMRNTNNHNREILKKSCLGVLLCEKKCIRPGTREYVVLRPAICDKARKKQMKNKCPN--CNGNLILQPCKGNLGFPVTHYWRRDEDRVFFQAKGIHDHLKPDLR 570
            +S  Q + E ++F  W DG  KL Y     E  + HISGWAM+ TNNHN+ +LKK+C+GVLLC K C  P   + +V+RPAI DK R++Q+   CPN  C+G L  + C GN G+PVTH+W   +D ++F++KG HDH +P  R
Sbjct:    2 ISTTQAVDEFDEFNEWIDGSCKLRYSAYSRE-AQAHISGWAMKYTNNHNKYVLKKTCVGVLLCSKDCTLPNGLK-IVVRPAISDKVRERQIGQNCPNASCSGILSHRKCTGNNGYPVTHFWVHQDDGIYFESKGTHDHFRPQAR 143
[Close]
BLAST of Transcription factor glial cells missing vs. Ensembl Nematostella
Match: EDO25565 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A8DW44])

HSP 1 Score: 138.272 bits (347), Expect = 9.940e-40
Identity = 67/141 (47.52%), Postives = 93/141 (65.96%), Query Frame = 1 
Query:  148 LSLPQLI-ETNDFQIWPDGHIKLVYDYSKNERVRRHISGWAMRNTNNHNREILKKSCLGVLLCEKKCIRPGTREYVVLRPAICDKARKKQMKNKCPN--CNGNLILQPCKGNLGFPVTHYWRRDEDRVFFQAKGIHDHLKP 561
            +S  Q + E ++F  W DG  KL Y     E  + HISGWAM+ TNNHN+ +LKK+C+GVLLC K C  P   + +V+RPAI DK R++Q+   CPN  C+G L  + C GN G+PVTH+W   +D ++F++KG HDH +P
Sbjct:    2 ISTTQAVDEFDEFNEWIDGSCKLRYSAYSRE-AQAHISGWAMKYTNNHNKYVLKKTCVGVLLCSKDCTLPNGLK-IVVRPAISDKVRERQIGQNCPNASCSGILSHRKCTGNNGYPVTHFWVHQDDGIYFESKGTHDHFRP 140
[Close]
BLAST of Transcription factor glial cells missing vs. Ensembl Medaka
Match: ENSORLT00000033677.1 (glial cells missing transcription factor 2 [Source:NCBI gene;Acc:101156002])

HSP 1 Score: 136.732 bits (343), Expect = 5.771e-36
Identity = 63/111 (56.76%), Postives = 83/111 (74.77%), Query Frame = 1 
Query:  268 MRNTNNHNREILKKSCLGVLLCEKKCIRP-GTREYVVLRPAICDKARKKQMKNKCPNCNGNLILQPCKGNLGFPVTHYWRRDEDRVFFQAKGIHDHLKPDLRPHRENRKKS 597
            MRNTNNHN +ILKKSCLGV++C + C  P G+R  + LRPAICDKAR+KQ K  CP+C+  L L PC+G+ G+PVT++WR D   +FFQAKG+HDH +P+ +   E R+ S
Sbjct:    1 MRNTNNHNCQILKKSCLGVVVCSRGCSLPDGSR--LQLRPAICDKARQKQQKKLCPSCSAGLELLPCRGHSGYPVTNFWRVDGKSIFFQAKGVHDHPRPESKSETEARRSS 109
[Close]
BLAST of Transcription factor glial cells missing vs. Planmine SMEST
Match: SMESG000036444.1 (SMESG000036444.1)

HSP 1 Score: 758.829 bits (1958), Expect = 0.000e+0
Identity = 365/368 (99.18%), Postives = 366/368 (99.46%), Query Frame = 1 
Query:   40 MVDNQGLPHLRMHLESSINIDVTATTPLINEWDVADLSLPQLIETNDFQIWPDGHIKLVYDYSKNERVRRHISGWAMRNTNNHNREILKKSCLGVLLCEKKCIRPGTREYVVLRPAICDKARKKQMKNKCPNCNGNLILQPCKGNLGFPVTHYWRRDEDRVFFQAKGIHDHLKPDLRPHRENRKKSSESNNVYMENNFLIHPSDSLSNNKKENYCSGCLQYNCMCQKNFENFRSQFFMPRRSPGMFWNPYQYPNTLYNSQYHKLPCYFMTPESSSYFHQQDPIFNSPIGYPPVLSPGNIPLIKSEELSVSNPFRIDPFLINHNTNQCDPVLNSDFSRQTPFQPSIFSISGRYPAHDALSNLSMPHSKY 1143
            MVDNQGLPHLRMHLESSINIDVTATTPLINEWDVADLSLPQLIETNDFQIWPDGHIKLVYDYSKNERVRRHISGWAMRNTNNHNREILKKSCLGVLLCEKKCIRPGTREYVVLRPAICDKARKKQMKNKCPNCNGNLILQPCKGNLGFPVTHYWRRDEDRVFFQAKGIHDHLKPDLRPHRENRKKSSESNNVYMENNFLIHPSDSLSNNKKENYCSGCLQYNCMCQKNFENFRSQFFMPRRSPGMFWNPYQYPNTLYNSQYHKLPCYFMTPESSSYFHQQ+PIFNSPIGYPPVLSPGNIPLIKSEELSVSNPFRIDPFLINHNTNQCDPVLNSDFSRQTPFQPSI SIS RYPAHDALSNLSMPHSKY
Sbjct:    1 MVDNQGLPHLRMHLESSINIDVTATTPLINEWDVADLSLPQLIETNDFQIWPDGHIKLVYDYSKNERVRRHISGWAMRNTNNHNREILKKSCLGVLLCEKKCIRPGTREYVVLRPAICDKARKKQMKNKCPNCNGNLILQPCKGNLGFPVTHYWRRDEDRVFFQAKGIHDHLKPDLRPHRENRKKSSESNNVYMENNFLIHPSDSLSNNKKENYCSGCLQYNCMCQKNFENFRSQFFMPRRSPGMFWNPYQYPNTLYNSQYHKLPCYFMTPESSSYFHQQEPIFNSPIGYPPVLSPGNIPLIKSEELSVSNPFRIDPFLINHNTNQCDPVLNSDFSRQTPFQPSISSISCRYPAHDALSNLSMPHSKY 368
[Close]
BLAST of Transcription factor glial cells missing vs. Planmine SMEST
Match: SMESG000056241.1 (SMESG000056241.1)

HSP 1 Score: 105.145 bits (261), Expect = 1.192e-24
Identity = 64/196 (32.65%), Postives = 96/196 (48.98%), Query Frame = 1 
Query:   70 RMHLESSINIDVTATTPLINEWDVADLSLPQLIETNDFQIWPDGHIKLVYDYSKNERVRRHISGWAMRNTNNHNREILKKSCLGVLLCEKKCIRPGTREYVVLRPAICDKARKKQMKNK-CPN--CNGNLILQPCKGNLGFPVTHYWRRDE--DRVFFQAKGIHDHLKPDLRPHRENRKKSSESNNVYMENNFLIH 642
              H  +S +  +  +   I++WD+ D  LPQ    + F++WPDG   L+Y+ + N + R+H                       V+ C K C  P     V L P I + AR+ Q+  K CPN  C+G LI Q C G  G PVTHYWR ++  + ++F A+G HDH KP+++    +++   E  N    N FL H
Sbjct:   59 EFHEHNSADAGILNSCFAISDWDIKDAVLPQPEFYHSFEMWPDGDCHLIYN-ATNNKARKHKR---------------------VIKCSKTCWYPNQNSIVRLAPKISEAARRCQLNKKPCPNPDCDGFLIQQLCTGKSGNPVTHYWRYNQHTNSIYFMARGSHDHEKPNIQIKMIDKRLKCEKLNFNELNEFLFH 232
[Close]
BLAST of Transcription factor glial cells missing vs. Planmine SMEST
Match: SMESG000056241.1 (SMESG000056241.1)

HSP 1 Score: 89.7373 bits (221), Expect = 1.913e-19
Identity = 55/159 (34.59%), Postives = 79/159 (49.69%), Query Frame = 1 
Query:  181 FQIWPDGHIKLVYDYSKNERVRRHISGWAMRNTNNHNREILKKSCLGVLLCEKKCIRPGTREYVVLRPAICDKARKKQMKNK-CPN--CNGNLILQPCKGNLGFPVTHYWRRDE--DRVFFQAKGIHDHLKPDLRPHRENRKKSSESNNVYMENNFLIH 642
            F++WPDG   L+Y+ + N + R+H                       V+ C K C  P     V L P I + AR+ Q+  K CPN  C+G LI Q C G  G PVTHYWR ++  + ++F A+G HDH KP+++    +++   E  N    N FL H
Sbjct:   80 FEMWPDGDCHLIYN-ATNNKARKHKR---------------------VIKCSKTCWYPNQNSIVRLAPKISEAARRCQLNKKPCPNPDCDGFLIQQLCTGKSGNPVTHYWRYNQHTNSIYFMARGSHDHEKPNIQIKMIDKRLKCEKLNFNELNEFLFH 216
The following BLAST results are available for this feature:
BLAST of Transcription factor glial cells missing vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 2
Match NameE-valueIdentityDescription
GCM11.860e-5150.91glial cells missing transcription factor 1 [Source... [more]
GCM26.649e-5152.23glial cells missing transcription factor 2 [Source... [more]
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BLAST of Transcription factor glial cells missing vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Transcription factor glial cells missing vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 3
Match NameE-valueIdentityDescription
gcm3.155e-5454.49gene:FBgn0014179 transcript:FBtr0335492[more]
gcm3.155e-5454.49gene:FBgn0014179 transcript:FBtr0079855[more]
gcm23.172e-5050.30gene:FBgn0019809 transcript:FBtr0079837[more]
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BLAST of Transcription factor glial cells missing vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
gcm25.679e-5152.56glial cells missing transcription factor 2 [Source... [more]
gcm26.332e-5152.56glial cells missing transcription factor 2 [Source... [more]
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BLAST of Transcription factor glial cells missing vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 3
Match NameE-valueIdentityDescription
gcm12.438e-5253.05glial cells missing transcription factor 1 [Source... [more]
GCM24.331e-4950.96glial cells missing homolog 2 [Source:NCBI gene;Ac... [more]
gcm15.381e-4852.98glial cells missing transcription factor 1 [Source... [more]
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BLAST of Transcription factor glial cells missing vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 3
Match NameE-valueIdentityDescription
Gcm21.355e-5246.33glial cells missing homolog 2 [Source:MGI Symbol;A... [more]
Gcm16.468e-5250.29glial cells missing homolog 1 [Source:MGI Symbol;A... [more]
Gcm21.955e-5046.89glial cells missing homolog 2 [Source:MGI Symbol;A... [more]
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BLAST of Transcription factor glial cells missing vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q27403|GCM_DROME3.162e-5354.49Transcription factor glial cells missing OS=Drosop... [more]
sp|P70348|GCM1_MOUSE4.524e-5150.29Chorion-specific transcription factor GCMa OS=Mus ... [more]
sp|Q9NP62|GCM1_HUMAN8.934e-5150.91Chorion-specific transcription factor GCMa OS=Homo... [more]
sp|O75603|GCM2_HUMAN3.193e-5052.23Chorion-specific transcription factor GCMb OS=Homo... [more]
sp|Q9Z288|GCM1_RAT4.763e-5052.87Chorion-specific transcription factor GCMa OS=Ratt... [more]
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BLAST of Transcription factor glial cells missing vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A1B0FMT73.007e-5250.83GCM domain-containing protein OS=Glossina morsitan... [more]
B3MUW73.108e-5253.66GCM domain-containing protein OS=Drosophila ananas... [more]
A0A1W4VHS33.128e-5254.49transcription factor glial cells missing OS=Drosop... [more]
A0A2R7W3I96.141e-5252.12Glial-cells-missing OS=Oncopeltus fasciatus OX=753... [more]
A0A482WKB26.560e-5254.88GCM domain-containing protein OS=Laodelphax striat... [more]
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BLAST of Transcription factor glial cells missing vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
gcm21.830e-4951.59glial cells missing transcription factor 2 [Source... [more]
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BLAST of Transcription factor glial cells missing vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 2
Match NameE-valueIdentityDescription
gcm21.522e-4952.53glial cells missing transcription factor 2 [Source... [more]
ENSPMAT00000001147.12.335e-1652.73pep scaffold:Pmarinus_7.0:GL476699:2797:4762:1 gen... [more]
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BLAST of Transcription factor glial cells missing vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Transcription factor glial cells missing vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 2
Match NameE-valueIdentityDescription
EDO332154.887e-4047.22Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO255659.940e-4047.52Predicted protein [Source:UniProtKB/TrEMBL;Acc:A8... [more]
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BLAST of Transcription factor glial cells missing vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ENSORLT00000033677.15.771e-3656.76glial cells missing transcription factor 2 [Source... [more]
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BLAST of Transcription factor glial cells missing vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 3
Match NameE-valueIdentityDescription
SMESG000036444.10.000e+099.18SMESG000036444.1[more]
SMESG000056241.11.192e-2432.65SMESG000056241.1[more]
SMESG000056241.11.913e-1934.59SMESG000056241.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30023953 ID=SMED30023953|Name=Transcription factor glial cells missing|organism=Schmidtea mediterranea sexual|type=transcript|length=1218bp
CGCCTTTTAGCAATATTTTAGTAGCCTTTTAGCAATATTATGGTGGATAA
TCAGGGTCTCCCTCATTTGAGAATGCATTTAGAATCATCAATTAATATAG
ATGTAACTGCCACCACCCCATTAATAAATGAATGGGATGTAGCGGATTTG
AGTTTACCACAGTTGATTGAAACAAATGACTTTCAAATATGGCCTGATGG
TCACATAAAATTGGTGTATGATTATTCGAAAAACGAAAGAGTCCGAAGAC
ATATAAGCGGTTGGGCGATGAGAAACACAAATAACCACAATCGAGAAATA
TTGAAGAAATCATGTCTTGGTGTATTGTTGTGTGAAAAAAAATGCATAAG
ACCGGGAACTAGAGAATATGTCGTATTGAGACCAGCGATTTGTGATAAAG
CTCGGAAAAAGCAAATGAAGAATAAATGCCCAAATTGCAATGGAAATTTG
ATATTGCAACCATGTAAAGGAAATCTTGGCTTTCCTGTCACTCATTATTG
GAGAAGAGACGAAGATCGAGTATTTTTTCAAGCGAAAGGAATTCATGATC
ATTTGAAGCCAGATTTACGACCTCATAGAGAAAATAGAAAGAAGTCTTCG
GAATCGAATAATGTATATATGGAAAATAATTTTTTAATCCATCCCTCAGA
TTCATTGTCAAATAATAAAAAAGAAAATTATTGCTCTGGATGTTTGCAGT
ATAATTGTATGTGCCAGAAAAATTTCGAAAATTTTCGATCTCAATTTTTT
ATGCCAAGACGATCGCCGGGAATGTTTTGGAATCCATATCAATATCCCAA
CACATTATACAATTCCCAATATCATAAACTTCCATGTTATTTCATGACTC
CCGAATCAAGTTCTTACTTTCACCAACAGGACCCGATTTTCAACAGTCCA
ATTGGTTATCCTCCGGTTTTGTCACCTGGGAATATCCCTCTCATTAAATC
CGAAGAGTTATCTGTTTCAAATCCATTTCGAATCGATCCGTTTCTCATCA
ATCACAACACAAATCAATGCGATCCGGTTTTGAATTCCGATTTCTCCCGC
CAAACACCTTTCCAACCATCCATTTTCTCAATCAGCGGTCGATATCCTGC
TCACGATGCTTTATCAAATCTATCAATGCCCCATTCCAAATACTGACCCA
AACCATACTTATTCCTCCCAAGATGTAGTTATCATTTCGTAAATAAATAT
TTCAATTTGTCAAAAAAA
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protein sequence of SMED30023953-orf-1

>SMED30023953-orf-1 ID=SMED30023953-orf-1|Name=SMED30023953-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=369bp
MVDNQGLPHLRMHLESSINIDVTATTPLINEWDVADLSLPQLIETNDFQI
WPDGHIKLVYDYSKNERVRRHISGWAMRNTNNHNREILKKSCLGVLLCEK
KCIRPGTREYVVLRPAICDKARKKQMKNKCPNCNGNLILQPCKGNLGFPV
THYWRRDEDRVFFQAKGIHDHLKPDLRPHRENRKKSSESNNVYMENNFLI
HPSDSLSNNKKENYCSGCLQYNCMCQKNFENFRSQFFMPRRSPGMFWNPY
QYPNTLYNSQYHKLPCYFMTPESSSYFHQQDPIFNSPIGYPPVLSPGNIP
LIKSEELSVSNPFRIDPFLINHNTNQCDPVLNSDFSRQTPFQPSIFSISG
RYPAHDALSNLSMPHSKY*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: molecular function
TermDefinition
GO:0001228transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
GO:0003677DNA binding
GO:0001077transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
GO:0001158enhancer sequence-specific DNA binding
GO:0003700transcription factor activity, sequence-specific DNA binding
GO:0005515protein binding
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000419prepharyngeal region
PLANA:0002109X1 cell
Vocabulary: INTERPRO
TermDefinition
IPR043020GCM_large
IPR043021GCM_small
IPR039791GCM
IPR036115GCM_dom_sf
IPR003902Tscrpt_reg_GCM
Vocabulary: biological process
TermDefinition
GO:0006355regulation of transcription, DNA-templated
GO:0006351transcription, DNA-templated
GO:0006366transcription from RNA polymerase II promoter
GO:0007275multicellular organism development
GO:0007403glial cell fate determination
GO:0007516hemocyte development
GO:0008283cell proliferation
GO:0010001glial cell differentiation
GO:0010468regulation of gene expression
GO:0010628positive regulation of gene expression
GO:0021782glial cell development
GO:0030097hemopoiesis
GO:0030182neuron differentiation
GO:0031290retinal ganglion cell axon guidance
GO:0035164embryonic plasmatocyte differentiation
GO:0035165embryonic crystal cell differentiation
GO:0035169lymph gland plasmatocyte differentiation
GO:0035289posterior head segmentation
GO:0042063gliogenesis
GO:0042387plasmatocyte differentiation
GO:0042690negative regulation of crystal cell differentiation
GO:0045610regulation of hemocyte differentiation
GO:0045687positive regulation of glial cell differentiation
GO:0045893positive regulation of transcription, DNA-templated
GO:0045944positive regulation of transcription from RNA polymerase II promoter
GO:0048813dendrite morphogenesis
GO:0050764regulation of phagocytosis
GO:0060252positive regulation of glial cell proliferation
GO:0060857establishment of glial blood-brain barrier
Vocabulary: cellular component
TermDefinition
GO:0005634nucleus
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003902Transcription regulator GCM domainPFAMPF03615GCMcoord: 46..183
e-value: 1.1E-60
score: 203.6
IPR003902Transcription regulator GCM domainPROSITEPS50807GCMcoord: 30..187
score: 53.343
IPR043020GCM, large domainGENE3DG3DSA:3.30.1370.90coord: 63..174
e-value: 3.9E-55
score: 187.0
IPR043021GCM, small domainGENE3DG3DSA:2.20.28.80coord: 95..184
e-value: 3.9E-55
score: 187.0
IPR039791Chorion-specific transcription factor GCMPANTHERPTHR12414GLIAL CELLS MISSING RELATED/GLIDEcoord: 30..224
NoneNo IPR availablePRODOMPD014393coord: 31..184
e-value: 2.0E-45
score: 461.0
IPR036115GCM domain superfamilySUPERFAMILYSSF90073GCM domaincoord: 31..184