slit1

Overview
Nameslit1 (ABC61956.2)
Smed IDSMED30023930
Length (bp)4812
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of slit1 (SMED30023930) t-SNE clustered cells

Violin plots show distribution of expression levels for slit1 (SMED30023930) in cells (dots) of each of the 12 neoblast clusters.

 

back to top


 

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of slit1 (SMED30023930) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for slit1 (SMED30023930) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

back to top


 

Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

back to top
Anatomical Expression

PAGE et. al., 2020




SMED30023930

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 15

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
midlineSMED30023930h1SMcG0013415 DQ336176smed_ncbi_20240424PMID:23318641
Chen et al., 2013
whole organism asexual adult fluorescence in situ hybridization evidence
dorsal midlineSMED30023930h1SMcG0013415 DQ336176smed_ncbi_20240424PMID:20707997
Gurley et al., 2010
whole organism asexual adult fluorescence in situ hybridization evidence
ventral midlineSMED30023930h1SMcG0013415 DQ336176smed_ncbi_20240424PMID:20707997
Gurley et al., 2010
whole organism asexual adult fluorescence in situ hybridization evidence
midlineSMED30023930h1SMcG0013415 DQ336176smed_ncbi_20240424PMID:20707997
Gurley et al., 2010
whole organism asexual adult fluorescence in situ hybridization evidence
muscle cellSMED30023930h1SMcG0013415 dd_Smed_v4_12111_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
midlineSMED30023930h1SMcG0013415 DQ336176smed_ncbi_20240424PMID:17553481
Cebrià et al., 2007
whole organism asexual adult colorimetric in situ hybridization evidence
ventral midlineSMED30023930h1SMcG0013415 DQ336176smed_ncbi_20240424PMID:21295483
Gaviño et al., 2011
whole organism asexual adult colorimetric in situ hybridization evidence
pharynxSMED30023930h1SMcG0013415 DQ336176smed_ncbi_20240424PMID:23318635
Blassberg et al., 2013
whole organism asexual adult colorimetric in situ hybridization evidence
pharynx pouchSMED30023930h1SMcG0013415 DQ336176smed_ncbi_20240424PMID:23318635
Blassberg et al., 2013
whole organism asexual adult colorimetric in situ hybridization evidence
midlineSMED30023930h1SMcG0013415 DQ336176smed_ncbi_20240424PMID:23318635
Blassberg et al., 2013
whole organism asexual adult colorimetric in situ hybridization evidence
midlineSMED30023930h1SMcG0013415 DQ336176smed_ncbi_20240424PMID:20422023
Felix et al., 2010
whole organism asexual adult colorimetric in situ hybridization evidence
Category 2 cellSMED30023930h1SMcG0013415 dd_Smed_v4_12111_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017
whole organism asexual adult single-cell RNA-sequencing evidence
dorsal midlineSMED30023930h1SMcG0013415 DQ336176smed_ncbi_20240424PMID:24415944
Scimone et al., 2014
whole organism asexual adult fluorescence in situ hybridization evidence
ventral midlineSMED30023930h1SMcG0013415 DQ336176smed_ncbi_20240424PMID:24415944
Scimone et al., 2014
whole organism asexual adult fluorescence in situ hybridization evidence
muscle cellSMED30023930h1SMcG0013415 dd_Smed_v4_12111_0_1dd_Smed_v4PMID:30962434
Cote et al., 2019
whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of slit1 vs. Ensembl Human
Match: SLIT3 (slit guidance ligand 3 [Source:HGNC Symbol;Acc:HGNC:11087])

HSP 1 Score: 723.776 bits (1867), Expect = 0.000e+0
Identity = 464/1375 (33.75%), Postives = 701/1375 (50.98%), Query Frame = 1
Query:  157 CPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQLQ------------------------CINDNIFNKLGMLKYLLLEGNKIFWI-SEAISKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCASEDKANLENMCPSDKKVCSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQIYNFQC--NKEFESKYETHDEECVPKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLPPIYSIHEAPNLMKLDLS------------------------FNQIETLEPDVFTRSSKLREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQFHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCDGNASVYHSVDSCHETNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVLSFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGN-YECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYC-NDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNSIYF-QKNSYIEISPPSRGFLMPRGNISLNFMTNSSSGLILYQGSEQTYLVVNLYQGHLRISYNLGDKVVGPEG--YSMGMVNDSRLHALHIEIVGENLTMYLNGRVHCTLKSKYLNSNRYMALDTGIFIGGAPHKILQSAIMKFHIETDSEFIGCMESLLINRKKIDFSKYLPKSINVHPGC 4113
            CP  C C   SV+C       VP  +     RL+L  N I  I K DF  L NL+VL L +N+++ IE GAF +L  L +LRLN+N +Q LP+ LF    KL +LDL +NQ+Q                        CI D  F  L  L+ L L  N I  I   + + +  ++ LR+H N L C+CHLAWL+ +L+Q+  +        LC  P  L    ++ +   E+ C +         C ++   C   C CS++ IV C GK L  IP +LP  +VE+ L+ N+I  +  G  ++YK LK+I +++N + DI P AF GL  LTS+ L  N +  I K  F GLVSL  L L  N I CL  +T +  + L+LL L +NKL ++ K  F P  +++ L+L  NP  CDC + W+ DY++D    T  A C  PR L    +  ++   F+C  ++++ S++ +   EC   + CP KC C   +V+CSN  L+ +P  +      L ++ N +  L       + PNL K++LS                         NQ+ET+   VF   S L+ + L SN + C++N+TF  L  + +L L +N I+ I   +F   ++L  + L  N F+C+C L W+ +W R    V GN   P CQ P  LK+ PI  +  + F CDGN        SC  +      C+                    T V CS+  L+ +P  +PKD TELYL+ NHL ++       L +L  + LS N I+ L     + +  L TL+LS+N+L+CI   AF  L +L+VL L  ND+S++P  +FNDL +L+++ALG NP HCDC+++WL+++    + + GI+ C+SPE M  + +  +    F C     D  I AKC++C+  PCK+NG C+      Y+C CPY + GK+C   IN C   PC++GG C   D++ + + C CP+GF G +C IN DDC  N C N ATC D  N+Y C C   + G+ CD    +C  ++N CQ+  +C  L +  + C C  G+ GK CE + DDC  + C+H +QCVD  N Y C C  G+ G FCE P   P VL                       +  S C  +EC N   CI       Q+  C+C PG++G  C+ L ++ F  K+SY+E+   +   + P+ NISL   T+  +G++LY+G +   L + LYQGH+R+ Y   D +  P    YS+  VND + H++ +  + + L + ++     +L    L     + +++ +++GG P     SA+ +        F GC+  + IN +  DF    P+S+ V PGC
Sbjct:   34 CPTKCTCSAASVDCHGLGLRAVPRGIPRNAERLDLDRNNITRITKMDFAGLKNLRVLHLEDNQVSVIERGAFQDLKQLERLRLNKNKLQVLPELLFQSTPKLTRLDLSENQIQGIPRKAFRGITDVKNLQLDNNHISCIEDGAFRALRDLEILTLNNNNISRILVTSFNHMPKIRTLRLHSNHLYCDCHLAWLSDWLRQRRTV----GQFTLCMAPVHLRGFNVADVQKKEYVCPAPHSE--PPSCNANSISCPSPCTCSNN-IVDCRGKGLMEIPANLPEGIVEIRLEQNSIKAIPAGAFTQYKKLKRIDISKNQISDIAPDAFQGLKSLTSLVLYGNKITEIVKGLFDGLVSLQLLLLNANKINCLRVNTFQDLQNLNLLSLYDNKLQTISKGLFAPLQSIQTLHLAQNPFVCDCHLKWLADYLQDNPIETSGARCSSPRRLANKRISQIKSKKFRCSGSEDYRSRFSS---ECFMDLVCPEKCRCEGTIVDCSNQKLVRIPSHLPEYVTDLRLNDNEVSVLEATGIFKKLPNLRKINLSNNKIKEVREGAFDGAASVQELMLTGNQLETVHGRVFRGLSGLKTLMLRSNLIGCVSNDTFAGLSSVRLLSLYDNRITTITPGAFTTLVSLSTINLLSNPFNCNCHLAWLGKWLRKRRIVSGN---PRCQKPFFLKEIPIQDVAIQDFTCDGNEE-----SSCQLSPRCPEQCTC-----------------METVVRCSNKGLRALPRGMPKDVTELYLEGNHLTAVPR-ELSALRHLTLIDLSNNSISMLTNYTFSNMSHLSTLILSYNRLRCIPVHAFNGLRSLRVLTLHGNDISSVPEGSFNDLTSLSHLALGTNPLHCDCSLRWLSEWVKAGYKEPGIARCSSPEPMADRLLLTTPTHRFQCKGPV-DINIVAKCNACLSSPCKNNGTCTQDPVELYRCACPYSYKGKDCTVPINTCIQNPCQHGGTCHLSDSHKDGFSCSCPLGFEGQRCEINPDDCEDNDCENNATCVDGINNYVCICPPNYTGELCDEVIDHCVPELNLCQHEAKCIPL-DKGFSCECVPGYSGKLCETDNDDCVAHKCRHGAQCVDTINGYTCTCPQGFSGPFCEHPP--PMVL-----------------------LQTSPCDQYECQNGAQCI----VVQQEPTCRCPPGFAGPRCEKLITVNFVGKDSYVEL---ASAKVRPQANISLQVATDKDNGILLYKG-DNDPLALELYQGHVRLVY---DSLSSPPTTVYSVETVNDGQFHSVELVTLNQTLNLVVDKGTPKSLGK--LQKQPAVGINSPLYLGGIPTSTGLSALRQGTDRPLGGFHGCIHEVRINNELQDFKALPPQSLGVSPGC 1332          

HSP 2 Score: 195.282 bits (495), Expect = 2.377e-49
Identity = 140/469 (29.85%), Postives = 229/469 (48.83%), Query Frame = 1
Query:  154 ICPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSD-FLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQLQCINDNIFNKLGMLKYLLLEGNKIFWISE-AISKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCASEDKANLENMCPSDKKVCSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQIYNFQCNKEFESKYETHDEECVPKVSCPLKCTCSSDVV 1554
            +CP  C+CE   V+C ++    +P  L E  T L L  N ++V+  +  F KL NL+ + LSNN+I  +  GAFD   ++ +L L  N ++ +   +F  L+ L+ L LR N + C++++ F  L  ++ L L  N+I  I+  A + L  L  + +  N  NCNCHLAWL  +L+++  +    + +  C  PF L  IPI  ++  +F C      N E+ C    + C ++C C  + +V+C  K L  +P+ +P +V EL L+ N++T + +  LS  + L  I L+ N +  +    F  ++ L+++ L+ N L+ I  +AF GL SL  L L  NDI  +P+ +              N LTSL            +L L  NPL+CDC + W+ +++K       +A C  P  +    L     + FQC    +         C+    C    TC+ D V
Sbjct:  504 VCPEKCRCEGTIVDCSNQKLVRIPSHLPEYVTDLRLNDNEVSVLEATGIFKKLPNLRKINLSNNKIKEVREGAFDGAASVQELMLTGNQLETVHGRVFRGLSGLKTLMLRSNLIGCVSNDTFAGLSSVRLLSLYDNRITTITPGAFTTLVSLSTINLLSNPFNCNCHLAWLGKWLRKRRIV----SGNPRCQKPFFLKEIPIQDVAIQDFTC----DGNEESSCQLSPR-CPEQCTC-METVVRCSNKGLRALPRGMPKDVTELYLEGNHLTAVPR-ELSALRHLTLIDLSNNSISMLTNYTFSNMSHLSTLILSYNRLRCIPVHAFNGLRSLRVLTLHGNDISSVPEGSF-------------NDLTSLS-----------HLALGTNPLHCDCSLRWLSEWVKAGYKEPGIARCSSPEPMADRLLLTTPTHRFQCKGPVDINIVAKCNACL-SSPCKNNGTCTQDPV 936          

HSP 3 Score: 182.956 bits (463), Expect = 1.433e-45
Identity = 128/479 (26.72%), Postives = 213/479 (44.47%), Query Frame = 1
Query: 1507 PKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLP-----------------------------------------------PIYSIHEAPNLMKLDLSFNQIETLEPDVFTRSSKLREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQFHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCDGNASVYHSVDSCHETNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVL------------------------SFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICS 2730
            P V+CP KCTCS+  V+C   GL ++P  I  + + L +  NN+ ++                                                P       P L +LDLS NQI+ +    F   + ++ + L++N + CI +  F+ L++LEIL L+ N IS I+  SF +   ++ L L+ N  +CDC L W+S+W R   TV    +   C +P+ L+   +  +  K ++C    S   S ++   + S  + C+  N                   VDC    L EIP  +P+   E+ L++N ++++   +F     LK + +S N I+++  +    LKSL +LVL                        + NK+ C+  + F++L NL +L L  N + TI    F  L+++  + L QNPF CDC++KWL  +  D  ++   + C+SP ++  K I   K   F CS
Sbjct:   30 PAVACPTKCTCSAASVDCHGLGLRAVPRGIPRNAERLDLDRNNITRITKMDFAGLKNLRVLHLEDNQVSVIERGAFQDLKQLERLRLNKNKLQVLPELLFQSTPKLTRLDLSENQIQGIPRKAFRGITDVKNLQLDNNHISCIEDGAFRALRDLEILTLNNNNISRILVTSFNHMPKIRTLRLHSNHLYCDCHLAWLSDWLRQRRTV---GQFTLCMAPVHLRGFNVADVQKKEYVCPAPHSEPPSCNA--NSISCPSPCTCSN-----------------NIVDCRGKGLMEIPANLPEGIVEIRLEQNSIKAIPAGAFTQYKKLKRIDISKNQISDIAPDAFQGLKSLTSLVLYGNKITEIVKGLFDGLVSLQLLLLNANKINCLRVNTFQDLQNLNLLSLYDNKLQTISKGLFAPLQSIQTLHLAQNPFVCDCHLKWLADYLQDNPIETSGARCSSPRRLANKRISQIKSKKFRCS 486          

HSP 4 Score: 53.5286 bits (127), Expect = 4.651e-6
Identity = 36/111 (32.43%), Postives = 55/111 (49.55%), Query Frame = 1
Query: 2917 GICKNIDNYGNYECVCPVGFRGPKCTINV-DDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCND-INPCQ----NSGQCQLLPNNDYKCVCSKGWEGKQCER 3231
            G+C++++   +  C C  G+ GP C     D C+G+ C +G  C     SY CKC  G+ G  CD K    ND  N C     + GQC +    +  C+C  G+ G+ C++
Sbjct: 1341 GLCRSVEK-DSVVCECRPGWTGPLCDQEARDPCLGHRCHHGK-CVATGTSYMCKCAEGYGGDLCDNK----NDSANACSAFKCHHGQCHISDQGEPYCLCQPGFSGEHCQQ 1445          
BLAST of slit1 vs. Ensembl Human
Match: SLIT3 (slit guidance ligand 3 [Source:HGNC Symbol;Acc:HGNC:11087])

HSP 1 Score: 720.694 bits (1859), Expect = 0.000e+0
Identity = 464/1381 (33.60%), Postives = 701/1381 (50.76%), Query Frame = 1
Query:  157 CPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQLQ------------------------CINDNIFNKLGMLKYLLLEGNKIFWI-SEAISKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCASEDKANLENMCPSDKKVCSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQIYNFQC--NKEFESKYETHDEECVPKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLPPIYSIHEAPNLMKLDLS------------------------FNQIETLEPDVFTRSSKLREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQFHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCDGNASVYHSVDSCHETNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVLSFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDF------ICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGN-YECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYC-NDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNSIYF-QKNSYIEISPPSRGFLMPRGNISLNFMTNSSSGLILYQGSEQTYLVVNLYQGHLRISYNLGDKVVGPEG--YSMGMVNDSRLHALHIEIVGENLTMYLNGRVHCTLKSKYLNSNRYMALDTGIFIGGAPHKILQSAIMKFHIETDSEFIGCMESLLINRKKIDFSKYLPKSINVHPGC 4113
            CP  C C   SV+C       VP  +     RL+L  N I  I K DF  L NL+VL L +N+++ IE GAF +L  L +LRLN+N +Q LP+ LF    KL +LDL +NQ+Q                        CI D  F  L  L+ L L  N I  I   + + +  ++ LR+H N L C+CHLAWL+ +L+Q+  +        LC  P  L    ++ +   E+ C +         C ++   C   C CS++ IV C GK L  IP +LP  +VE+ L+ N+I  +  G  ++YK LK+I +++N + DI P AF GL  LTS+ L  N +  I K  F GLVSL  L L  N I CL  +T +  + L+LL L +NKL ++ K  F P  +++ L+L  NP  CDC + W+ DY++D    T  A C  PR L    +  ++   F+C  ++++ S++ +   EC   + CP KC C   +V+CSN  L+ +P  +      L ++ N +  L       + PNL K++LS                         NQ+ET+   VF   S L+ + L SN + C++N+TF  L  + +L L +N I+ I   +F   ++L  + L  N F+C+C L W+ +W R    V GN   P CQ P  LK+ PI  +  + F CDGN        SC  +      C+                    T V CS+  L+ +P  +PKD TELYL+ NHL ++       L +L  + LS N I+ L     + +  L TL+LS+N+L+CI   AF  L +L+VL L  ND+S++P  +FNDL +L+++ALG NP HCDC+++WL+++    + + GI+ C+SPE M  + +  +    F       C     D  I AKC++C+  PCK+NG C+      Y+C CPY + GK+C   IN C   PC++GG C   D++ + + C CP+GF G +C IN DDC  N C N ATC D  N+Y C C   + G+ CD    +C  ++N CQ+  +C  L +  + C C  G+ GK CE + DDC  + C+H +QCVD  N Y C C  G+ G FCE P   P VL                       +  S C  +EC N   CI       Q+  C+C PG++G  C+ L ++ F  K+SY+E+   +   + P+ NISL   T+  +G++LY+G +   L + LYQGH+R+ Y   D +  P    YS+  VND + H++ +  + + L + ++     +L    L     + +++ +++GG P     SA+ +        F GC+  + IN +  DF    P+S+ V PGC
Sbjct:   34 CPTKCTCSAASVDCHGLGLRAVPRGIPRNAERLDLDRNNITRITKMDFAGLKNLRVLHLEDNQVSVIERGAFQDLKQLERLRLNKNKLQVLPELLFQSTPKLTRLDLSENQIQGIPRKAFRGITDVKNLQLDNNHISCIEDGAFRALRDLEILTLNNNNISRILVTSFNHMPKIRTLRLHSNHLYCDCHLAWLSDWLRQRRTV----GQFTLCMAPVHLRGFNVADVQKKEYVCPAPHSE--PPSCNANSISCPSPCTCSNN-IVDCRGKGLMEIPANLPEGIVEIRLEQNSIKAIPAGAFTQYKKLKRIDISKNQISDIAPDAFQGLKSLTSLVLYGNKITEIVKGLFDGLVSLQLLLLNANKINCLRVNTFQDLQNLNLLSLYDNKLQTISKGLFAPLQSIQTLHLAQNPFVCDCHLKWLADYLQDNPIETSGARCSSPRRLANKRISQIKSKKFRCSGSEDYRSRFSS---ECFMDLVCPEKCRCEGTIVDCSNQKLVRIPSHLPEYVTDLRLNDNEVSVLEATGIFKKLPNLRKINLSNNKIKEVREGAFDGAASVQELMLTGNQLETVHGRVFRGLSGLKTLMLRSNLIGCVSNDTFAGLSSVRLLSLYDNRITTITPGAFTTLVSLSTINLLSNPFNCNCHLAWLGKWLRKRRIVSGN---PRCQKPFFLKEIPIQDVAIQDFTCDGNEE-----SSCQLSPRCPEQCTC-----------------METVVRCSNKGLRALPRGMPKDVTELYLEGNHLTAVPR-ELSALRHLTLIDLSNNSISMLTNYTFSNMSHLSTLILSYNRLRCIPVHAFNGLRSLRVLTLHGNDISSVPEGSFNDLTSLSHLALGTNPLHCDCSLRWLSEWVKAGYKEPGIARCSSPEPMADRLLLTTPTHRFQCKVLWFCCPGPVDINIVAKCNACLSSPCKNNGTCTQDPVELYRCACPYSYKGKDCTVPINTCIQNPCQHGGTCHLSDSHKDGFSCSCPLGFEGQRCEINPDDCEDNDCENNATCVDGINNYVCICPPNYTGELCDEVIDHCVPELNLCQHEAKCIPL-DKGFSCECVPGYSGKLCETDNDDCVAHKCRHGAQCVDTINGYTCTCPQGFSGPFCEHPP--PMVL-----------------------LQTSPCDQYECQNGAQCI----VVQQEPTCRCPPGFAGPRCEKLITVNFVGKDSYVEL---ASAKVRPQANISLQVATDKDNGILLYKG-DNDPLALELYQGHVRLVY---DSLSSPPTTVYSVETVNDGQFHSVELVTLNQTLNLVVDKGTPKSLGK--LQKQPAVGINSPLYLGGIPTSTGLSALRQGTDRPLGGFHGCIHEVRINNELQDFKALPPQSLGVSPGC 1339          

HSP 2 Score: 194.897 bits (494), Expect = 2.959e-49
Identity = 134/437 (30.66%), Postives = 220/437 (50.34%), Query Frame = 1
Query:  154 ICPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSD-FLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQLQCINDNIFNKLGMLKYLLLEGNKIFWISE-AISKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCASEDKANLENMCPSDKKVCSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQIYNFQC 1458
            +CP  C+CE   V+C ++    +P  L E  T L L  N ++V+  +  F KL NL+ + LSNN+I  +  GAFD   ++ +L L  N ++ +   +F  L+ L+ L LR N + C++++ F  L  ++ L L  N+I  I+  A + L  L  + +  N  NCNCHLAWL  +L+++  +    + +  C  PF L  IPI  ++  +F C      N E+ C    + C ++C C  + +V+C  K L  +P+ +P +V EL L+ N++T + +  LS  + L  I L+ N +  +    F  ++ L+++ L+ N L+ I  +AF GL SL  L L  NDI  +P+ +              N LTSL            +L L  NPL+CDC + W+ +++K       +A C  P  +    L     + FQC
Sbjct:  504 VCPEKCRCEGTIVDCSNQKLVRIPSHLPEYVTDLRLNDNEVSVLEATGIFKKLPNLRKINLSNNKIKEVREGAFDGAASVQELMLTGNQLETVHGRVFRGLSGLKTLMLRSNLIGCVSNDTFAGLSSVRLLSLYDNRITTITPGAFTTLVSLSTINLLSNPFNCNCHLAWLGKWLRKRRIV----SGNPRCQKPFFLKEIPIQDVAIQDFTC----DGNEESSCQLSPR-CPEQCTC-METVVRCSNKGLRALPRGMPKDVTELYLEGNHLTAVPR-ELSALRHLTLIDLSNNSISMLTNYTFSNMSHLSTLILSYNRLRCIPVHAFNGLRSLRVLTLHGNDISSVPEGSF-------------NDLTSLS-----------HLALGTNPLHCDCSLRWLSEWVKAGYKEPGIARCSSPEPMADRLLLTTPTHRFQC 905          

HSP 3 Score: 182.956 bits (463), Expect = 1.556e-45
Identity = 128/479 (26.72%), Postives = 213/479 (44.47%), Query Frame = 1
Query: 1507 PKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLP-----------------------------------------------PIYSIHEAPNLMKLDLSFNQIETLEPDVFTRSSKLREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQFHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCDGNASVYHSVDSCHETNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVL------------------------SFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICS 2730
            P V+CP KCTCS+  V+C   GL ++P  I  + + L +  NN+ ++                                                P       P L +LDLS NQI+ +    F   + ++ + L++N + CI +  F+ L++LEIL L+ N IS I+  SF +   ++ L L+ N  +CDC L W+S+W R   TV    +   C +P+ L+   +  +  K ++C    S   S ++   + S  + C+  N                   VDC    L EIP  +P+   E+ L++N ++++   +F     LK + +S N I+++  +    LKSL +LVL                        + NK+ C+  + F++L NL +L L  N + TI    F  L+++  + L QNPF CDC++KWL  +  D  ++   + C+SP ++  K I   K   F CS
Sbjct:   30 PAVACPTKCTCSAASVDCHGLGLRAVPRGIPRNAERLDLDRNNITRITKMDFAGLKNLRVLHLEDNQVSVIERGAFQDLKQLERLRLNKNKLQVLPELLFQSTPKLTRLDLSENQIQGIPRKAFRGITDVKNLQLDNNHISCIEDGAFRALRDLEILTLNNNNISRILVTSFNHMPKIRTLRLHSNHLYCDCHLAWLSDWLRQRRTV---GQFTLCMAPVHLRGFNVADVQKKEYVCPAPHSEPPSCNA--NSISCPSPCTCSN-----------------NIVDCRGKGLMEIPANLPEGIVEIRLEQNSIKAIPAGAFTQYKKLKRIDISKNQISDIAPDAFQGLKSLTSLVLYGNKITEIVKGLFDGLVSLQLLLLNANKINCLRVNTFQDLQNLNLLSLYDNKLQTISKGLFAPLQSIQTLHLAQNPFVCDCHLKWLADYLQDNPIETSGARCSSPRRLANKRISQIKSKKFRCS 486          

HSP 4 Score: 53.1434 bits (126), Expect = 5.372e-6
Identity = 36/111 (32.43%), Postives = 55/111 (49.55%), Query Frame = 1
Query: 2917 GICKNIDNYGNYECVCPVGFRGPKCTINV-DDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCND-INPCQ----NSGQCQLLPNNDYKCVCSKGWEGKQCER 3231
            G+C++++   +  C C  G+ GP C     D C+G+ C +G  C     SY CKC  G+ G  CD K    ND  N C     + GQC +    +  C+C  G+ G+ C++
Sbjct: 1348 GLCRSVEK-DSVVCECRPGWTGPLCDQEARDPCLGHRCHHGK-CVATGTSYMCKCAEGYGGDLCDNK----NDSANACSAFKCHHGQCHISDQGEPYCLCQPGFSGEHCQQ 1452          
BLAST of slit1 vs. Ensembl Human
Match: SLIT2 (slit guidance ligand 2 [Source:HGNC Symbol;Acc:HGNC:11086])

HSP 1 Score: 714.146 bits (1842), Expect = 0.000e+0
Identity = 487/1483 (32.84%), Postives = 735/1483 (49.56%), Query Frame = 1
Query:  151 EICPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQLQ------------------------CINDNIFNKLGMLKYLLLEGNKIFWISEA-ISKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCASEDKANLENMCPSDKKV-CSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQIYNFQCN--KEFESKYETHDEECVPKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLPPIYSIHEAPNLMKLDLSFNQIETLEPDVFTRSSKLREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQ------------------------FHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCD-GNASVYHSVDSCHETNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVLSFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGIC--KNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYC-NDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNSIYF-QKNSYIEISPPSRGFLMPRGNISLNFMTNSSSGLILYQGSEQTYLVVNLYQGHLRISYNLGDKVVGPEGYSMGMVNDSRLHALHIEIVGENLTMYLNGRVHCTLKSKYLNSNRYMALDTGIFIGGAPHKILQSAIMKFHIETDSEFIGCMESLLINRKKIDFSKYLPKSINVHPGCGKLISTVVASDLKIPSIMXXXXXXXXXXXXXXXNNVCKRPGICKNQGVCEVLPGSKKKFRCICKNGFVGRKCKKRKSMCHGTFTESHIVDPDSQGTCKSIERFQY 4428
            + CP  C C   +V+C      +VP  +   T RL+L GN I  I K+DF  L +L+VLQL  N+I++IE GAF +L  L +LRLNRN +Q  P+ LF    KL +LDL +NQ+Q                        CI D  F  L  L+ L L  N I  +S A  + +  L+  R+H N L C+CHLAWL+ +L+Q+  +   G ++  C  P  L    ++ +   EF C+ E++ +   M PS   + C   C CS++ IV C GK L+ IP +LP  + E+ L+ N I ++  G  S YK L++I L+ N + ++ P AF GL  L S+ L  N +  + K+ F GL SL  L L  N I CL  D  +    L+LL L +NKL ++ K TF P   ++ ++L  NP  CDC + W+ DY+      T  A C  PR L    +  ++   F+C+  +++ SK      +C   ++CP KC C    V+CSN  L  +PE I   T  L +++N    L       + P L K++ S N+I  +E   F  +S + EI L SNRL+ + ++ FK L+ L+ L L  N I+C+  DSF    +++ L L  NQ                        F+C+C L W+ EW R    V GN   P CQ P  LK+ PI  +  + F CD GN       +SC   +   T C+                    T V CS+  LK +P  IP+D TELYLD N   +L      N  +L  + LS N I+ L+    + +  L TL+LS+N+L+CI    F  L +L++L L  ND+S +P  AFNDL  L+++A+G NP +CDCN++WL+ +    + + GI+ CA P +M  K +  +    F C     D  I AKC+ C+  PCK++G C+      Y+C CPY F G++CD  I+AC   PC++GG C  K  +  G + C+C  GF G  C +NVDDC  N C N +TC D  N+Y+C C   + G+ C+ K  +C  D+NPCQ+  +C L P   +KC C+ G+ G+ C+ + DDC+ N CK+ + C D  N Y C+C  GY G FCE     P VL                          S C   +C N   CI     +  +  C+C PGY G  C+ L S+ F  K SY++I P ++  + P+ NI+L   T+  SG++LY+G ++ ++ V LY+G +R SY+ G        YS+  +ND   H + +  + ++L++ ++G     + +  L+    +  D+ +++GG P K   +++ +   +  + F GC+ +L IN +  DF K +P    + PGC      V A                                     G C+  P S+  F C C+ G++G  C +R +       +  + +    GTC  I  F Y
Sbjct:   26 QACPAQCSCSGSTVDCHGLALRSVPRNIPRNTERLDLNGNNITRITKTDFAGLRHLRVLQLMENKISTIERGAFQDLKELERLRLNRNHLQLFPELLFLGTAKLYRLDLSENQIQAIPRKAFRGAVDIKNLQLDYNQISCIEDGAFRALRDLEVLTLNNNNITRLSVASFNHMPKLRTFRLHSNNLYCDCHLAWLSDWLRQRPRV---GLYTQ-CMGPSHLRGHNVAEVQKREFVCSDEEEGHQSFMAPSCSVLHCPAACTCSNN-IVDCRGKGLTEIPTNLPETITEIRLEQNTIKVIPPGAFSPYKKLRRIDLSNNQISELAPDAFQGLRSLNSLVLYGNKITELPKSLFEGLFSLQLLLLNANKINCLRVDAFQDLHNLNLLSLYDNKLQTIAKGTFSPLRAIQTMHLAQNPFICDCHLKWLADYLHTNPIETSGARCTSPRRLANKRIGQIKSKKFRCSGTEDYRSKLSG---DCFADLACPEKCRCEGTTVDCSNQKLNKIPEHIPQYTAELRLNNNEFTVLEATGIFKKLPQLRKINFSNNKITDIEEGAFEGASGVNEILLTSNRLENVQHKMFKGLESLKTLMLRSNRITCVGNDSFIGLSSVRLLSLYDNQITTVAPGAFDTLHSLSTLNLLANPFNCNCYLAWLGEWLRKKRIVTGN---PRCQKPYFLKEIPIQDVAIQDFTCDDGNDD-----NSCSPLSRCPTECTC-----------------LDTVVRCSNKGLKVLPKGIPRDVTELYLDGNQF-TLVPKELSNYKHLTLIDLSNNRISTLSNQSFSNMTQLLTLILSYNRLRCIPPRTFDGLKSLRLLSLHGNDISVVPEGAFNDLSALSHLAIGANPLYCDCNMQWLSDWVKSEYKEPGIARCAGPGEMADKLLLTTPSKKFTCQGPV-DVNILAKCNPCLSNPCKNDGTCNSDPVDFYRCTCPYGFKGQDCDVPIHACISNPCKHGGTCHLKEGEEDG-FWCICADGFEGENCEVNVDDCEDNDCENNSTCVDGINNYTCLCPPEYTGELCEEKLDFCAQDLNPCQHDSKCILTPKG-FKCDCTPGYVGEHCDIDFDDCQDNKCKNGAHCTDAVNGYTCICPEGYSGLFCEFSP--PMVLP-----------------------RTSPCDNFDCQNGAQCI----VRINEPICQCLPGYQGEKCEKLVSVNFINKESYLQI-PSAK--VRPQTNITLQIATDEDSGILLYKG-DKDHIAVELYRGRVRASYDTGSHPASAI-YSVETINDGNFHIVELLALDQSLSLSVDGGNPKIITN--LSKQSTLNFDSPLYVGGMPGKSNVASLRQAPGQNGTSFHGCIRNLYINSELQDFQK-VPMQTGILPGCEPCHKKVCA------------------------------------HGTCQ--PSSQAGFTCECQEGWMGPLCDQRTN-------DPCLGNKCVHGTCLPINAFSY 1389          

HSP 2 Score: 58.9214 bits (141), Expect = 1.038e-7
Identity = 43/111 (38.74%), Postives = 56/111 (50.45%), Query Frame = 1
Query: 2917 GICKNIDNYGNYECVCPVGFRGPKCTINVDD-CVGNLCVNGATCNDLNN-SYSCKCQSGFRGKYCDIKFLYCNDINPCQ----NSGQCQLLPNNDYKCVCSKGWEGKQCER 3231
            G C+     G + C C  G+ GP C    +D C+GN CV+G TC  +N  SYSCKC  G  G  CD +    +  NPCQ      G+C+L       C CS G+ G  C+R
Sbjct: 1340 GTCQPSSQAG-FTCECQEGWMGPLCDQRTNDPCLGNKCVHG-TCLPINAFSYSCKCLEGHGGVLCDEEE---DLFNPCQAIKCKHGKCRLSGLGQPYCECSSGYTGDSCDR 1445          
BLAST of slit1 vs. Ensembl Human
Match: SLIT2 (slit guidance ligand 2 [Source:HGNC Symbol;Acc:HGNC:11086])

HSP 1 Score: 705.671 bits (1820), Expect = 0.000e+0
Identity = 486/1483 (32.77%), Postives = 731/1483 (49.29%), Query Frame = 1
Query:  151 EICPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQLQ------------------------CINDNIFNKLGMLKYLLLEGNKIFWISEA-ISKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCASEDKANLENMCPSDKKV-CSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQIYNFQCN--KEFESKYETHDEECVPKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLPPIYSIHEAPNLMKLDLSFNQIETLEPDVFTRSSKLREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQ------------------------FHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCD-GNASVYHSVDSCHETNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVLSFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGIC--KNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYC-NDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNSIYF-QKNSYIEISPPSRGFLMPRGNISLNFMTNSSSGLILYQGSEQTYLVVNLYQGHLRISYNLGDKVVGPEGYSMGMVNDSRLHALHIEIVGENLTMYLNGRVHCTLKSKYLNSNRYMALDTGIFIGGAPHKILQSAIMKFHIETDSEFIGCMESLLINRKKIDFSKYLPKSINVHPGCGKLISTVVASDLKIPSIMXXXXXXXXXXXXXXXNNVCKRPGICKNQGVCEVLPGSKKKFRCICKNGFVGRKCKKRKSMCHGTFTESHIVDPDSQGTCKSIERFQY 4428
            + CP  C C   +V+C      +VP  +   T RL+L GN I  I K+DF  L +L+VLQL  N+I++IE GAF +L  L +LRLNRN +Q  P+ LF    KL +LDL +NQ+Q                        CI D  F  L  L+ L L  N I  +S A  + +  L+  R+H N L C+CHLAWL+ +L+Q+  +   G ++  C  P  L    ++ +   EF C+         M PS   + C   C CS++ IV C GK L+ IP +LP  + E+ L+ N I ++  G  S YK L++I L+ N + ++ P AF GL  L S+ L  N +  + K+ F GL SL  L L  N I CL  D  +    L+LL L +NKL ++ K TF P   ++ ++L  NP  CDC + W+ DY+      T  A C  PR L    +  ++   F+C+  +++ SK      +C   ++CP KC C    V+CSN  L  +PE I   T  L +++N    L       + P L K++ S N+I  +E   F  +S + EI L SNRL+ + ++ FK L+ L+ L L  N I+C+  DSF    +++ L L  NQ                        F+C+C L W+ EW R    V GN   P CQ P  LK+ PI  +  + F CD GN       +SC   +   T C+                    T V CS+  LK +P  IP+D TELYLD N   +L      N  +L  + LS N I+ L+    + +  L TL+LS+N+L+CI    F  L +L++L L  ND+S +P  AFNDL  L+++A+G NP +CDCN++WL+ +    + + GI+ CA P +M  K +  +    F C     D  I AKC+ C+  PCK++G C+      Y+C CPY F G++CD  I+AC   PC++GG C  K  +  G + C+C  GF G  C +NVDDC  N C N +TC D  N+Y+C C   + G+ C+ K  +C  D+NPCQ+  +C L P   +KC C+ G+ G+ C+ + DDC+ N CK+ + C D  N Y C+C  GY G FCE     P VL                          S C   +C N   CI     +  +  C+C PGY G  C+ L S+ F  K SY++I P ++  + P+ NI+L   T+  SG++LY+G ++ ++ V LY+G +R SY+ G        YS+  +ND   H + +  + ++L++ ++G     + +  L+    +  D+ +++GG P K   +++ +   +  + F GC+ +L IN +  DF K +P    + PGC      V A                                     G C+  P S+  F C C+ G++G  C +R +       +  + +    GTC  I  F Y
Sbjct:   26 QACPAQCSCSGSTVDCHGLALRSVPRNIPRNTERLDLNGNNITRITKTDFAGLRHLRVLQLMENKISTIERGAFQDLKELERLRLNRNHLQLFPELLFLGTAKLYRLDLSENQIQAIPRKAFRGAVDIKNLQLDYNQISCIEDGAFRALRDLEVLTLNNNNITRLSVASFNHMPKLRTFRLHSNNLYCDCHLAWLSDWLRQRPRV---GLYTQ-CMGPSHLRGHNVAEVQKREFVCSGHQSF----MAPSCSVLHCPAACTCSNN-IVDCRGKGLTEIPTNLPETITEIRLEQNTIKVIPPGAFSPYKKLRRIDLSNNQISELAPDAFQGLRSLNSLVLYGNKITELPKSLFEGLFSLQLLLLNANKINCLRVDAFQDLHNLNLLSLYDNKLQTIAKGTFSPLRAIQTMHLAQNPFICDCHLKWLADYLHTNPIETSGARCTSPRRLANKRIGQIKSKKFRCSGTEDYRSKLSG---DCFADLACPEKCRCEGTTVDCSNQKLNKIPEHIPQYTAELRLNNNEFTVLEATGIFKKLPQLRKINFSNNKITDIEEGAFEGASGVNEILLTSNRLENVQHKMFKGLESLKTLMLRSNRITCVGNDSFIGLSSVRLLSLYDNQITTVAPGAFDTLHSLSTLNLLANPFNCNCYLAWLGEWLRKKRIVTGN---PRCQKPYFLKEIPIQDVAIQDFTCDDGNDD-----NSCSPLSRCPTECTC-----------------LDTVVRCSNKGLKVLPKGIPRDVTELYLDGNQF-TLVPKELSNYKHLTLIDLSNNRISTLSNQSFSNMTQLLTLILSYNRLRCIPPRTFDGLKSLRLLSLHGNDISVVPEGAFNDLSALSHLAIGANPLYCDCNMQWLSDWVKSEYKEPGIARCAGPGEMADKLLLTTPSKKFTCQGPV-DVNILAKCNPCLSNPCKNDGTCNSDPVDFYRCTCPYGFKGQDCDVPIHACISNPCKHGGTCHLKEGEEDG-FWCICADGFEGENCEVNVDDCEDNDCENNSTCVDGINNYTCLCPPEYTGELCEEKLDFCAQDLNPCQHDSKCILTPKG-FKCDCTPGYVGEHCDIDFDDCQDNKCKNGAHCTDAVNGYTCICPEGYSGLFCEFSP--PMVLP-----------------------RTSPCDNFDCQNGAQCI----VRINEPICQCLPGYQGEKCEKLVSVNFINKESYLQI-PSAK--VRPQTNITLQIATDEDSGILLYKG-DKDHIAVELYRGRVRASYDTGSHPASAI-YSVETINDGNFHIVELLALDQSLSLSVDGGNPKIITN--LSKQSTLNFDSPLYVGGMPGKSNVASLRQAPGQNGTSFHGCIRNLYINSELQDFQK-VPMQTGILPGCEPCHKKVCA------------------------------------HGTCQ--PSSQAGFTCECQEGWMGPLCDQRTN-------DPCLGNKCVHGTCLPINAFSY 1385          

HSP 2 Score: 58.9214 bits (141), Expect = 1.029e-7
Identity = 43/111 (38.74%), Postives = 56/111 (50.45%), Query Frame = 1
Query: 2917 GICKNIDNYGNYECVCPVGFRGPKCTINVDD-CVGNLCVNGATCNDLNN-SYSCKCQSGFRGKYCDIKFLYCNDINPCQ----NSGQCQLLPNNDYKCVCSKGWEGKQCER 3231
            G C+     G + C C  G+ GP C    +D C+GN CV+G TC  +N  SYSCKC  G  G  CD +    +  NPCQ      G+C+L       C CS G+ G  C+R
Sbjct: 1336 GTCQPSSQAG-FTCECQEGWMGPLCDQRTNDPCLGNKCVHG-TCLPINAFSYSCKCLEGHGGVLCDEEE---DLFNPCQAIKCKHGKCRLSGLGQPYCECSSGYTGDSCDR 1441          
BLAST of slit1 vs. Ensembl Human
Match: SLIT2 (slit guidance ligand 2 [Source:HGNC Symbol;Acc:HGNC:11086])

HSP 1 Score: 704.131 bits (1816), Expect = 0.000e+0
Identity = 487/1500 (32.47%), Postives = 736/1500 (49.07%), Query Frame = 1
Query:  151 EICPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQLQ------------------------CINDNIFNKLGMLKYLLLEGNKIFWISEA-ISKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCASEDKANLENMCPSDKKV-CSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQIYNFQCN----------KEFESKYETHDEECVPKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLPPIYSIHEAPNLMKLDLSFNQIETLEPDVFTRSSKLREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQ------------------------FHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCD-GNASVYHSVDSCHETNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVLSFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGIC--KNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGF---------RGKYCDIKFLYC-NDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNSIYF-QKNSYIEISPPSRGFLMPRGNISLNFMTNSSSGLILYQGSEQTYLVVNLYQGHLRISYNLGDKVVGPEGYSMGMVNDSRLHALHIEIVGENLTMYLNGRVHCTLKSKYLNSNRYMALDTGIFIGGAPHKILQSAIMKFHIETDSEFIGCMESLLINRKKIDFSKYLPKSINVHPGCGKLISTVVASDLKIPSIMXXXXXXXXXXXXXXXNNVCKRPGICKNQGVCEVLPGSKKKFRCICKNGFVGRKCKKRKSMCHGTFTESHIVDPDSQGTCKSIERFQY 4428
            + CP  C C   +V+C      +VP  +   T RL+L GN I  I K+DF  L +L+VLQL  N+I++IE GAF +L  L +LRLNRN +Q  P+ LF    KL +LDL +NQ+Q                        CI D  F  L  L+ L L  N I  +S A  + +  L+  R+H N L C+CHLAWL+ +L+Q+  +   G ++  C  P  L    ++ +   EF C+ E++ +   M PS   + C   C CS++ IV C GK L+ IP +LP  + E+ L+ N I ++  G  S YK L++I L+ N + ++ P AF GL  L S+ L  N +  + K+ F GL SL  L L  N I CL  D  +    L+LL L +NKL ++ K TF P   ++ ++L  NP  CDC + W+ DY+      T  A C  PR L    +  ++   F+C+          +++ SK      +C   ++CP KC C    V+CSN  L  +PE I   T  L +++N    L       + P L K++ S N+I  +E   F  +S + EI L SNRL+ + ++ FK L+ L+ L L  N I+C+  DSF    +++ L L  NQ                        F+C+C L W+ EW R    V GN   P CQ P  LK+ PI  +  + F CD GN       +SC   +   T C+                    T V CS+  LK +P  IP+D TELYLD N   +L      N  +L  + LS N I+ L+    + +  L TL+LS+N+L+CI    F  L +L++L L  ND+S +P  AFNDL  L+++A+G NP +CDCN++WL+ +    + + GI+ CA P +M  K +  +    F C     D  I AKC+ C+  PCK++G C+      Y+C CPY F G++CD  I+AC   PC++GG C  K  +  G + C+C  GF G  C +NVDDC  N C N +TC D  N+Y+C C   +         +G+ C+ K  +C  D+NPCQ+  +C L P   +KC C+ G+ G+ C+ + DDC+ N CK+ + C D  N Y C+C  GY G FCE     P VL                          S C   +C N   CI     +  +  C+C PGY G  C+ L S+ F  K SY++I P ++  + P+ NI+L   T+  SG++LY+G ++ ++ V LY+G +R SY+ G        YS+  +ND   H + +  + ++L++ ++G     + +  L+    +  D+ +++GG P K   +++ +   +  + F GC+ +L IN +  DF K +P    + PGC      V A                                     G C+  P S+  F C C+ G++G  C +R +       +  + +    GTC  I  F Y
Sbjct:   26 QACPAQCSCSGSTVDCHGLALRSVPRNIPRNTERLDLNGNNITRITKTDFAGLRHLRVLQLMENKISTIERGAFQDLKELERLRLNRNHLQLFPELLFLGTAKLYRLDLSENQIQAIPRKAFRGAVDIKNLQLDYNQISCIEDGAFRALRDLEVLTLNNNNITRLSVASFNHMPKLRTFRLHSNNLYCDCHLAWLSDWLRQRPRV---GLYTQ-CMGPSHLRGHNVAEVQKREFVCSDEEEGHQSFMAPSCSVLHCPAACTCSNN-IVDCRGKGLTEIPTNLPETITEIRLEQNTIKVIPPGAFSPYKKLRRIDLSNNQISELAPDAFQGLRSLNSLVLYGNKITELPKSLFEGLFSLQLLLLNANKINCLRVDAFQDLHNLNLLSLYDNKLQTIAKGTFSPLRAIQTMHLAQNPFICDCHLKWLADYLHTNPIETSGARCTSPRRLANKRIGQIKSKKFRCSAKEQYFIPGTEDYRSKLSG---DCFADLACPEKCRCEGTTVDCSNQKLNKIPEHIPQYTAELRLNNNEFTVLEATGIFKKLPQLRKINFSNNKITDIEEGAFEGASGVNEILLTSNRLENVQHKMFKGLESLKTLMLRSNRITCVGNDSFIGLSSVRLLSLYDNQITTVAPGAFDTLHSLSTLNLLANPFNCNCYLAWLGEWLRKKRIVTGN---PRCQKPYFLKEIPIQDVAIQDFTCDDGNDD-----NSCSPLSRCPTECTC-----------------LDTVVRCSNKGLKVLPKGIPRDVTELYLDGNQF-TLVPKELSNYKHLTLIDLSNNRISTLSNQSFSNMTQLLTLILSYNRLRCIPPRTFDGLKSLRLLSLHGNDISVVPEGAFNDLSALSHLAIGANPLYCDCNMQWLSDWVKSEYKEPGIARCAGPGEMADKLLLTTPSKKFTCQGPV-DVNILAKCNPCLSNPCKNDGTCNSDPVDFYRCTCPYGFKGQDCDVPIHACISNPCKHGGTCHLKEGEEDG-FWCICADGFEGENCEVNVDDCEDNDCENNSTCVDGINNYTCLCPPEYTAANLNEVEKGELCEEKLDFCAQDLNPCQHDSKCILTPKG-FKCDCTPGYVGEHCDIDFDDCQDNKCKNGAHCTDAVNGYTCICPEGYSGLFCEFSP--PMVLP-----------------------RTSPCDNFDCQNGAQCI----VRINEPICQCLPGYQGEKCEKLVSVNFINKESYLQI-PSAK--VRPQTNITLQIATDEDSGILLYKG-DKDHIAVELYRGRVRASYDTGSHPASAI-YSVETINDGNFHIVELLALDQSLSLSVDGGNPKIITN--LSKQSTLNFDSPLYVGGMPGKSNVASLRQAPGQNGTSFHGCIRNLYINSELQDFQK-VPMQTGILPGCEPCHKKVCA------------------------------------HGTCQ--PSSQAGFTCECQEGWMGPLCDQRTN-------DPCLGNKCVHGTCLPINAFSY 1406          

HSP 2 Score: 58.9214 bits (141), Expect = 9.559e-8
Identity = 43/111 (38.74%), Postives = 56/111 (50.45%), Query Frame = 1
Query: 2917 GICKNIDNYGNYECVCPVGFRGPKCTINVDD-CVGNLCVNGATCNDLNN-SYSCKCQSGFRGKYCDIKFLYCNDINPCQ----NSGQCQLLPNNDYKCVCSKGWEGKQCER 3231
            G C+     G + C C  G+ GP C    +D C+GN CV+G TC  +N  SYSCKC  G  G  CD +    +  NPCQ      G+C+L       C CS G+ G  C+R
Sbjct: 1357 GTCQPSSQAG-FTCECQEGWMGPLCDQRTNDPCLGNKCVHG-TCLPINAFSYSCKCLEGHGGVLCDEEE---DLFNPCQAIKCKHGKCRLSGLGQPYCECSSGYTGDSCDR 1462          
BLAST of slit1 vs. Ensembl Celegans
Match: slt-1 (Slit homolog 1 protein [Source:UniProtKB/Swiss-Prot;Acc:G5EFX6])

HSP 1 Score: 627.476 bits (1617), Expect = 0.000e+0
Identity = 452/1534 (29.47%), Postives = 706/1534 (46.02%), Query Frame = 1
Query:  100 LYCFSSTIWINDPSDESEICPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQLQCINDNIFNKLGMLKYLLLEGNKIF---------WISEAISKL-------------KHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCASEDKANLENMCPSDKKVCSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQIYNFQCNKEFESKYETHDEECVPKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLPPIYSIHEAPNLMKLDLSFNQIETLEPDVFTRSSKLREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQFHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCDGNASVYHSVDSCHE-TNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVLSFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCN-DINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNSIYFQK-NSYIEISPPSRGFLMPRGNISLNFMTNSSSGLILYQGSEQTYLVVNLYQGHLRISYNLGDKVVGPEGYSMGMVNDSRLHALHIEIVGENLTMYLNGRVHCTLKSKYLNSNRYMALDTGIFIGGAPHKILQSAIMKFHIETDSEFIGCMESLLINRKKIDFSKYLPKSINVHPGCGKLISTVVASDLKIPSIMXXXXXXXXXXXXXXXNNVCKRPGICKNQGVCEVLPGSKKKFRCICKNGFVGRKCKKRKSMCHGT-FTESHIVDPDSQGTCKSIERFQYRICTGTCSTSRMQSDVIKNFYHKPIDPSSSRCCQPTKYSTHEIQFKCENGRVYRRAANLPKNCKC 4623
            L CF   + I     ES  CP  C C + +V C  +    VP  +   T RL+L  N I  I  +DF  L NLK LQL +N+I +I   +F +LV L KLRL+RN I++LPD++F    KL  LDL +N +  ++D        L+ L L+ N IF         W+S  +  L                ++L +  N  NC+C L W+  +L++       G +  +C+TP  L    I  L      C+   K   +  C +  ++C   C C+    V C    L+ +P +LP +  E+ L+ N I+ +        K L ++ L++N + +I+P AFLGL  L ++ L  NN+  ++ + F GL SL  L L  N + C+ + T +   +L +L L +N + S+ + TF+   +L  L+L  NPL CDC + W+      K   T  A C QP+ L+      L    F+C K  ES    + + C     CP +C C    V+C+  GL ++P SI      L++S NN+  +    +IH   NL  LDLS N I  +    F + SKLRE+ LN N+L   ++        LEIL LS N I C  +  F     ++ + +  N   CDC++  +  W R++ +   +  +P CQ           + D++    D         ++C + +N     CS  +                   V CS+  L   P+ IP DTTELYLD N++  +       L +L  L LS+N +  L  N  + L  L TL++S+NKL+C+   AF  L+ L++L L  ND+S +P  AF++L ++ +IA+G N  +CDCN+ W +++   +F++ GI+ C  P  +  + +  ++P  F C  +      T KCD C+  PCK+N  C   +   Y C+C   F+G +C+ +I+AC+G PC N   CK +   G + C C  GF G  C  N+DDCV + C NG  C DL NSY C C   + GK+C+ K  YC   +NPC+N+G+C +  N  Y C+CS G+ G  CE N DDCK   C++   CVD    Y C+CRPGY G++CEIP                    M  E  + +   +S C   EC+         QN +  + CKC  G+SG  C    S+ F+   +Y+ + P     L   G I++   T S  G++LY G +  ++   LY G +++ Y +G+       YS   VND   H + I        + ++      +++   +          ++IGG P +  Q A  +FH++      GC+ S+ IN   I+  + L +++N    C   +                              N C   GI    G C     S K + C C + F G  C +++  C    F   HI     +  C+S++R +   C G C   +                    CC   K    +++  C+NG       ++ + C+C
Sbjct:    2 LICFIFILLI----PESATCPAECVCVDRTVSCVGQQLTEVPQNIPNDTIRLDLQDNEITKIGPNDFSSLMNLKALQLMDNQIVTIHNQSFSSLVFLQKLRLSRNRIRHLPDNVFQNNLKLTHLDLSENDITVVSDAQLQGPEFLEVLNLDKNHIFCLENNVISSWVSLEVLTLNGNRLTTFEEPSNARFRQLDLFNNPWNCDCRLRWMRKWLEKAE-----GQNKTVCATPLNLQGSSIEILQDKFMTCSGNRKRRYKKTCET-AEICPLPCTCT-GTTVDCRDSGLTYVPTNLPPSTTEIRLEQNQISSIPSHSFKNLKNLTRLDLSKNIITEIQPKAFLGLHNLHTLVLYGNNITDLKSDTFEGLGSLQLLLLNANQLTCIRRGTFDHVPKLSMLSLYDNDIKSISEVTFQNLTSLSTLHLAKNPLICDCNLQWLAQINLQKNIETSGARCEQPKRLRKKKFATLPPNKFKC-KGSESFVSMYADSCFIDSICPTQCDCYGTTVDCNKRGLNTIPTSIPRFATQLLLSGNNISTVDLNSNIHVLENLEVLDLSNNHITFINDKSFEKLSKLRELRLNDNKLHHFSSMVLDEQSNLEILDLSGNNIQCFSSIFFNKATRIREIKVIGNDLLCDCRILPLMSWLRSNSS--HSIDIPPCQQ--------FQYSDNES---DKQRCAAFPEETCSDDSNLCPPKCSCLDRV-----------------VRCSNKNLTSFPSRIPFDTTELYLDANYINEIPAHDLNRLYSLTKLDLSHNRLISLENNTFSNLTRLSTLIISYNKLRCLQPLAFNGLNALRILSLHGNDISFLPQSAFSNLTSITHIAVGSNSLYCDCNMAWFSKWIKSKFIEAGIARCEYPNTVSNQLLLTAQPYQFTCDSKVPTKLAT-KCDLCLNSPCKNNAICETTSSRKYTCNCTPGFYGVHCENQIDACYGSPCLNNATCK-VAQAGRFNCYCNKGFEGDYCEKNIDDCVNSKCENGGKCVDLINSYRCDCPMEYEGKHCEDKLEYCTKKLNPCENNGKC-IPINGSYSCMCSPGFTGNNCETNIDDCKNVECQNGGSCVDGILSYDCLCRPGYAGQYCEIPPM------------------MDMEYQKTDACQQSACGQGECV-------ASQNSSDFT-CKCHEGFSGPSCDRQMSVGFKNPGAYLALDP-----LASDGTITMTLRTTSKIGILLYYGDDH-FVSAELYDGRVKLVYYIGN-FPASHMYSSVKVNDGLPHRISIRTSERKCFLQIDKNPVQIVENSGKSDQLITKGKEMLYIGGLPIEKSQDAKRRFHVKNSESLKGCISSITINEVPINLQQAL-ENVNTEQSCSATV------------------------------NFCA--GIDCGNGKCTNNALSPKGYMCQCDSHFSGEHCDEKRIKCDKQKFRRHHI-----ENECRSVDRIKIAECNGYCGGEQ-------------------NCCTAVKKKQRKVKMICKNGTTKISTVHIIRQCQC 1400          
BLAST of slit1 vs. Ensembl Celegans
Match: clec-78 (C-type LECtin [Source:UniProtKB/TrEMBL;Acc:Q20531])

HSP 1 Score: 159.844 bits (403), Expect = 8.237e-39
Identity = 100/276 (36.23%), Postives = 137/276 (49.64%), Query Frame = 1
Query: 2773 CMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNN--SYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIP----AWKPTV----LKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYL 3570
            C  + C++N +CS+  H   QC C   + G  C+   + C  QPC NGG C+ +     Y+C CP  F G +C    D+C G  C NG  C+DL    S +C C++GF G  C+     C+  NPC+N  +C       +KC C  GWEG  C++N DDC  + C   + CVDL NDYKC C  G+ GK C I     A  P V    +  L SR+ + Q   T EN +  +     CA   C N+  CI    ++     C+C  GY G  CQ+L
Sbjct: 2017 CSAKMCENNAECSVFMHR-AQCHCKPGYVGDRCEMLEDVCSTQPCYNGGKCEQVGT--TYKCTCPKMFNGARCQFESDECNGVKCPNGGVCHDLPGVKSTTCLCRTGFAGPQCEEITDICSTNNPCRNGARCIGEKLGRFKCQCVPGWEGPNCDKNIDDCADSPCALNATCVDLINDYKCECPTGFSGKRCHIKENLCASSPCVHGLCIDKLYSRQCLCQPGWTGENCDQNI---DECAASPCQNDAKCI----DEINGYMCECADGYEGVHCQHL 2282          

HSP 2 Score: 133.265 bits (334), Expect = 1.226e-30
Identity = 91/277 (32.85%), Postives = 133/277 (48.01%), Query Frame = 1
Query: 2767 DSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKH--ESQCVDLHNDYKCMCRPGYIGKFCEIP----AWKPTV-----LKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQ 3564
            D C   PC  N  C  + + +Y+C+CP  F GK C  + N C   PC +G +C  ID   + +C+C  G+ G  C  N+D+C  + C N A C D  N Y C+C  G+ G +C     +C    PC N+  C  +    Y C C+ G++G  CE N D+C  N C       C D  ND+KC+C+PGY G  C++     A  P +     +    + + + +S  T    E +  S   CA   C NNG C++   +     +C C PG SG  C+
Sbjct: 2171 DDCADSPCALNATCVDLIN-DYKCECPTGFSGKRCHIKENLCASSPCVHG-LC--IDKLYSRQCLCQPGWTGENCDQNIDECAASPCQNDAKCIDEINGYMCECADGYEGVHCQHLVDHCAK-QPCHNNATCTNM-GATYHCDCTLGFDGVHCEMNIDECAENQCDKLGTESCRDAVNDFKCVCKPGYTGPRCDVKQDQCADSPCLNDAQCVDMGGAYKCVCKSGWTGPKCEQDNGS---CAAKPCRNNGFCVSLVADY----FCVCPPGVSGKNCE 2434          

HSP 3 Score: 124.02 bits (310), Expect = 6.399e-28
Identity = 76/243 (31.28%), Postives = 112/243 (46.09%), Query Frame = 1
Query: 2746 AYITAKC----DSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNI----------------------------------------DNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCE 3342
             Y+  +C    D C  +PC + GKC  +    Y+C CP  F+G  C    + C G  C NGG+C ++                                        +  G ++C C  G+ GP C  N+DDC  + C   ATC DL N Y C+C +GF GK C IK   C   +PC + G C +      +C+C  GW G+ C++N D+C  + C+++++C+D  N Y C C  GY G  C+
Sbjct: 2042 GYVGDRCEMLEDVCSTQPCYNGGKCEQV-GTTYKCTCPKMFNGARCQFESDECNGVKCPNGGVCHDLPGVKSTTCLCRTGFAGPQCEEITDICSTNNPCRNGARCIGEKLGRFKCQCVPGWEGPNCDKNIDDCADSPCALNATCVDLINDYKCECPTGFSGKRCHIKENLCAS-SPCVH-GLC-IDKLYSRQCLCQPGWTGENCDQNIDECAASPCQNDAKCIDEINGYMCECADGYEGVHCQ 2280          

HSP 4 Score: 121.709 bits (304), Expect = 3.250e-27
Identity = 85/281 (30.25%), Postives = 119/281 (42.35%), Query Frame = 1
Query: 2734 ETEDAYITAKC----DSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQ 3564
            E  D Y    C    D C K+PC +N  C+ +    Y CDC   F G +C+  I+ C    C   G     D   +++CVC  G+ GP+C +  D C  + C+N A C D+  +Y C C+SG+ G  C+     C    PC+N+G C  L   DY CVC  G  GK CE   + C    C +  +C D  +  +C C   + GK CE   +K T  KT                               C N G C        +   C+C PG++G  C+
Sbjct: 2269 ECADGYEGVHCQHLVDHCAKQPCHNNATCTNM-GATYHCDCTLGFDGVHCEMNIDECAENQCDKLGTESCRDAVNDFKCVCKPGYTGPRCDVKQDQCADSPCLNDAQCVDMGGAYKCVCKSGWTGPKCEQDNGSCA-AKPCRNNGFCVSL-VADYFCVCPPGVSGKNCESAPNRCIGQPCHNGGECGDFGSHLECACPASFTGKGCE---FKNTGCKT-------------------------------CENGGKCAEAAGGLQK---CECSPGFTGERCE 2509          

HSP 5 Score: 119.013 bits (297), Expect = 2.370e-26
Identity = 79/257 (30.74%), Postives = 114/257 (44.36%), Query Frame = 1
Query: 2587 FHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIY-HSKPTDFICSEETEDAYITAKCD----SCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCE 3342
            +HCDC +      F     +  I  CA  +  KL +        DF C    +  Y   +CD     C   PC ++ +C +     Y+C C   + G  C++   +C  +PCRN G C ++    +Y CVCP G  G  C    + C+G  C NG  C D  +   C C + F GK C+ K   C     C+N G+C        KC CS G+ G++CE N D+C    C   + CVD  N + C+C     G  C+
Sbjct: 2304 YHCDCTLG-----FDGVHCEMNIDECAENQCDKLGTESCRDAVNDFKCV--CKPGYTGPRCDVKQDQCADSPCLNDAQC-VDMGGAYKCVCKSGWTGPKCEQDNGSCAAKPCRNNGFCVSL--VADYFCVCPPGVSGKNCESAPNRCIGQPCHNGGECGDFGSHLECACPASFTGKGCEFKNTGCKT---CENGGKCAEAAGGLQKCECSPGFTGERCETNIDECSTAHCPSGATCVDQVNTHICVCPFNLTGVHCD 2547          
BLAST of slit1 vs. Ensembl Celegans
Match: Y46G5A.29 (pep chromosome:WBcel235:II:12856200:12862058:1 gene:WBGene00012917.1 transcript:Y46G5A.29.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:Y46G5A.29)

HSP 1 Score: 112.849 bits (281), Expect = 7.483e-25
Identity = 67/156 (42.95%), Postives = 91/156 (58.33%), Query Frame = 1
Query: 2890 CFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATC-NDLNN--SYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIP 3348
            C   PC+NGG C    + G   C C  G+ G  CT  +D C+ N C N +TC ND  +   YSCKCQ+G  GK C+I+   C   + C+N GQC L       C C  G  G++CER  ++C +NSCK+ ++CVDL NDY+C C  G++G+ CE P
Sbjct:  370 CQDNPCQNGGRCSM--SKGKSTCQCNPGYVGANCT-EIDKCLDNECDNNSTCFNDKRSPVGYSCKCQNGTVGKLCEIE---CPK-DQCENGGQCYLNTFGKIGCKCVPGTTGRRCEREINECGWNSCKNGAKCVDLFNDYRCDCADGWMGRNCERP 518          

HSP 2 Score: 80.1073 bits (196), Expect = 9.165e-15
Identity = 77/277 (27.80%), Postives = 116/277 (41.88%), Query Frame = 1
Query: 2479 HNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYG-NYECVCPVGFRGPKCTINVDDCVGNLCVNGATCN-DLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSC---KHESQCVDLHN 3294
            H+ K LI + ++ + +  + F     LN+  L ++      ++K+LN     R L +                      DF+ + + E        + C   PC++ G+CS+ +     C C   + G NC + I+ C    C N   C N       Y C C  G  G  C I   +C  + C NG  C  +      CKC  G  G+ C+ +   C   N C+N  +C  L  NDY+C C+ GW G+ CER   D  Y SC   K E QC ++ N
Sbjct:  306 HSSKFLIEKPSESTFMMGRMFKKFAPLNDTVLQED------DLKFLNSHVHHRMLQH----------------------DFVDNNDLEME------EKCQDNPCQNGGRCSM-SKGKSTCQCNPGYVGANCTE-IDKCLDNECDNNSTCFNDKRSPVGYSCKCQNGTVGKLCEI---ECPKDQCENGGQCYLNTFGKIGCKCVPGTTGRRCEREINECG-WNSCKNGAKCVDL-FNDYRCDCADGWMGRNCERPCQDI-YGSCRVWKREGQCQEMRN 540          
BLAST of slit1 vs. Ensembl Celegans
Match: glp-1 (pep chromosome:WBcel235:III:9092241:9099698:1 gene:WBGene00001609.1 transcript:F02A9.6.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:glp-1)

HSP 1 Score: 106.686 bits (265), Expect = 1.098e-22
Identity = 83/299 (27.76%), Postives = 130/299 (43.48%), Query Frame = 1
Query: 2722 ICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDC-VGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCE-----------------RNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQ 3564
            IC+     +Y     D C +  C     C + + +NY CDCP    G+ C++   A  G  C +G    N D   N+ CVC  G+ G  C  + ++C +   CVN +TC +L+  ++C C+ G+ GKYC+     C D   CQN G C    N    C C +G+ G++CE                 R        +C ++ +C++      C+C P YIG  CE                 IN+ +  + ++++       C Y+ C+NN  CI+    KN    C C  G+ G  C+
Sbjct:  141 ICNNGYGGSYCEEGIDHCAQNECAEGSTC-VNSVYNYYCDCPIGKSGRYCERTECALMGNICNHGRCIPNRDEDKNFRCVCDSGYEGEFCNKDKNECLIEETCVNNSTCFNLHGDFTCTCKPGYAGKYCEEAIDMCKDY-VCQNDGYCAHDSNQMPICYCEQGFTGQRCEIECPSGFGGIHCDLPLQRPHCSRSNGTCYNDGRCINGF----CVCEPDYIGDRCE-----------------INRKDFKFPDIQS-------CKYNPCVNNATCID---LKNSGYSCHCPLGFYGLNCE 406          

HSP 2 Score: 105.531 bits (262), Expect = 2.125e-22
Identity = 77/248 (31.05%), Postives = 115/248 (46.37%), Query Frame = 1
Query: 2839 DCPYP-FHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNND----YKCVCSKGWEGKQCERNTDDCKY-NSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQ 3564
            DC  P F G      +N C   PC NG +C     YG ++C+C  G+ G  C   +D C  N C  G+TC +   +Y C C  G  G+YC+     C  +    N G+C  +PN D    ++CVC  G+EG+ C ++ ++C    +C + S C +LH D+ C C+PGY GK+CE                              E I   +C  + C N+G C +   + NQ   C C+ G++G  C+
Sbjct:  109 DCKTPLFSG------VNPCDSDPCNNG-LCYPF--YGGFQCICNNGYGGSYCEEGIDHCAQNECAEGSTCVNSVYNYYCDCPIGKSGRYCE--RTECALMGNICNHGRC--IPNRDEDKNFRCVCDSGYEGEFCNKDKNECLIEETCVNNSTCFNLHGDFTCTCKPGYAGKYCE------------------------------EAI--DMCKDYVCQNDGYCAH---DSNQMPICYCEQGFTGQRCE 308          

HSP 3 Score: 89.3521 bits (220), Expect = 1.844e-17
Identity = 76/267 (28.46%), Postives = 115/267 (43.07%), Query Frame = 1
Query: 2773 CMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATC--NDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYK-CMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQ 3564
            C   PC +NG C    +  +QC C   + G  C++ I+ C    C  G  C  +++  NY C CP+G  G  C       +GN+C +G      D + ++ C C SG+ G++C+     C     C N+  C  L + D+ C C  G+ GK CE   D CK   C+++  C    N    C C  G+ G+ CEI    P+    +     + +   +  N               C N+G CING        +C C+P Y G  C+
Sbjct:  121 CDSDPC-NNGLCYPF-YGGFQCICNNGYGGSYCEEGIDHCAQNECAEGSTC--VNSVYNYYCDCPIGKSGRYCERTECALMGNICNHGRCIPNRDEDKNFRCVCDSGYEGEFCNKDKNECLIEETCVNNSTCFNL-HGDFTCTCKPGYAGKYCEEAIDMCKDYVCQNDGYCAHDSNQMPICYCEQGFTGQRCEIEC--PSGFGGIHCDLPLQRPHCSRSN-------------GTCYNDGRCING--------FCVCEPDYIGDRCE 359          

HSP 4 Score: 83.9593 bits (206), Expect = 9.014e-16
Identity = 93/301 (30.90%), Postives = 129/301 (42.86%), Query Frame = 1
Query: 2716 DFICSEETEDA--YITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFG---------QP--CRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDD--------CVGNLCVNGATCNDLNNS-YSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCE-RNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCE----IPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCK 3537
            DF C+ +   A  Y     D C    C+++G C+  ++    C C   F G+ C+    + FG         +P   R+ G C N     N  CVC   + G +C IN  D        C  N CVN ATC DL NS YSC C  GF G  C+ + L C     C N G C+ + N   +C C  G+ G  CE ++   C  + CK+   C    N   C C+ GY G  CE    I   K TV++ L  +R          +L +  I    C   EC  +G    GD +  Q+ + KC+
Sbjct:  254 DFTCTCKPGYAGKYCEEAIDMCKDYVCQNDGYCAHDSNQMPICYCEQGFTGQRCEIECPSGFGGIHCDLPLQRPHCSRSNGTCYNDGRCINGFCVCEPDYIGDRCEINRKDFKFPDIQSCKYNPCVNNATCIDLKNSGYSCHCPLGFYGLNCE-QHLLCTPTT-CANGGTCEGV-NGVIRCNCPNGFSGDYCEIKDRQLCSRHPCKNGGVC---KNTGYCECQYGYTGPTCEEVLVIEKSKETVIRDLCEQRKC-------MDLASNGICNPECNLEECNFDG----GDCSGGQRPFSKCQ 537          

HSP 5 Score: 76.2554 bits (186), Expect = 2.014e-13
Identity = 81/316 (25.63%), Postives = 122/316 (38.61%), Query Frame = 1
Query: 2722 ICSEETEDAYITAKCDSCM-KRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNL------------CVNGATCNDLN--NSYSCKCQSGFRGKYCDI--KFLYCNDI-----NPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNSIYFQKNSYI 3603
            +C    E  +     + C+ +  C +N  C    H ++ C C   + GK C++ I+ C    C+N G C +  N     C C  GF G +C I      G +              NG   ND    N + C C+  + G  C+I  K     DI     NPC N+  C  L N+ Y C C  G+ G  CE++   C   +C +   C  ++   +C C  G+ G +CEI                                   +C+ H C N G+C N         YC+C+ GY+G  C+ +  I   K + I
Sbjct:  219 VCDSGYEGEFCNKDKNECLIEETCVNNSTC-FNLHGDFTCTCKPGYAGKYCEEAIDMCKDYVCQNDGYCAHDSNQMPI-CYCEQGFTGQRCEIECPSGFGGIHCDLPLQRPHCSRSNGTCYNDGRCINGF-CVCEPDYIGDRCEINRKDFKFPDIQSCKYNPCVNNATCIDLKNSGYSCHCPLGFYGLNCEQHLL-CTPTTCANGGTCEGVNGVIRCNCPNGFSGDYCEIK-------------------------------DRQLCSRHPCKNGGVCKN-------TGYCECQYGYTGPTCEEVLVIEKSKETVI 492          

HSP 6 Score: 59.6918 bits (143), Expect = 2.227e-8
Identity = 71/257 (27.63%), Postives = 100/257 (38.91%), Query Frame = 1
Query: 2905 CRNGGICKNIDNYGNYECVCPVGFRGPKCTINVD-DC-VGNLCVNGA-------TCNDLNNSYSCK------CQSGFRGKYCDIKFLYCNDINPCQ----NSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTL------ISRRDINQS-----EMTYENL-----ENEVISESVCAYHECLNNGICIN--GDQNKNQKSYCKCKPGYSGHLCQ 3564
            C   G C N      Y C C  GF G  C    D DC  G  C+           C D  +           C+ G+ G  C       + +NPC     N+G C       ++C+C+ G+ G  CE   D C  N C   S CV+   +Y C C  G  G++CE    +  ++  +      I  RD +++     +  YE       +NE + E       C+NN  C N  GD        C CKPGY+G  C+
Sbjct:   29 CSVNGKCYNGKLIETYWCRCKKGFGGAFCERECDLDCKRGEKCIYDVYGENPTCICQDCEDETPPTERTQKGCEEGYGGPDCKTPLF--SGVNPCDSDPCNNGLCYPF-YGGFQCICNNGYGGSYCEEGIDHCAQNECAEGSTCVNSVYNYYCDCPIGKSGRYCE--RTECALMGNICNHGRCIPNRDEDKNFRCVCDSGYEGEFCNKDKNECLIEET-----CVNNSTCFNLHGDFT------CTCKPGYAGKYCE 269          
BLAST of slit1 vs. Ensembl Celegans
Match: crb-1 (Drosophila CRumBs homolog [Source:UniProtKB/TrEMBL;Acc:Q19350])

HSP 1 Score: 104.76 bits (260), Expect = 4.185e-22
Identity = 83/287 (28.92%), Postives = 126/287 (43.90%), Query Frame = 1
Query: 2767 DSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCR-PGYIGKFCE--------------------IPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSY-CKCKPGYSGHLC 3561
            + C    C   G CS +  +N  C+C   + G +C  +++ C   PC N G+C+  D    + C C   F G +C I    C+   CV+G        +++C C  G+ G  CD K  YC    PC N   C+      YKC C+ G+EG  CE N D+C    CK+ ++C D  NDY+C+C   G+ G+ C                     +  +K       I  R   ++  T +   N  IS   C + +C+N  + I  ++N+    Y C C  GY+G  C
Sbjct: 1369 NQCSSNTCSSRGACSPV-WNNTVCNCDNNWRGAHCQHQMDTCLDFPCNNDGVCRTNDE-NTFSCECQKFFMGTRCEIE-GSCLKAQCVHGECIQLSPETHTCSCNIGYEGDACDKKIDYCK-AGPCLNGANCENKLTG-YKCTCAVGFEGADCEINIDECALEFCKNGAKCRDKINDYECVCDGTGFEGRNCTTDINECANPNNCINGECTNTLGNYKCACRNGFIGPRCSVRNPCTAQIASNN-ISSVTCVHGKCVNPVVQI--EKNREVAKYECACDRGYTGPTC 1647          

HSP 2 Score: 104.375 bits (259), Expect = 5.212e-22
Identity = 74/222 (33.33%), Postives = 105/222 (47.30%), Query Frame = 1
Query: 2734 ETEDAYITAKCD---SCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQ-SGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCE-RN------------TDDCKYNSCKHESQCVDLHND---YKCMCRPGYIGKFC 3339
            E +  ++  +C+   SC+K  C H G+C  ++   + C C   + G  CDK+I+ C   PC NG  C+N      Y+C C VGF G  C IN+D+C    C NGA C D  N Y C C  +GF G+ C      C + N C N G+C     N YKC C  G+ G +C  RN            +  C +  C +    ++ + +   Y+C C  GY G  C
Sbjct: 1431 ECQKFFMGTRCEIEGSCLKAQCVH-GECIQLSPETHTCSCNIGYEGDACDKKIDYCKAGPCLNGANCEN--KLTGYKCTCAVGFEGADCEINIDECALEFCKNGAKCRDKINDYECVCDGTGFEGRNCTTDINECANPNNCIN-GECTNTLGN-YKCACRNGFIGPRCSVRNPCTAQIASNNISSVTCVHGKCVNPVVQIEKNREVAKYECACDRGYTGPTC 1647          

HSP 3 Score: 103.605 bits (257), Expect = 1.059e-21
Identity = 127/485 (26.19%), Postives = 201/485 (41.44%), Query Frame = 1
Query: 2806 CSLI--THHNYQCDCPYPFHGKNCDK-RINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCV-GNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLY-----CNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTL-------ISRRD-INQSEMTYENLEN-----EVISESVCAYHE------------------CLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNSIYFQKNSYIEISPPSRGFLMPRGNISLNFMTNSSSGLILYQGSEQTYLVVNLYQGHLRISYN-LGDKVVGPE---GY-SMGMVNDSRLHALHIEIV-GENLTMYLNGRVHCTLKSKYLNSNRYMALDTGIFIGGAPHKILQSAIMKF-HIETDSEFIGCMESLLINRKKIDFSKYLPKSINVHPGCGK 4119
            CSL+  T    QC+CP  F G +C + +   C  +PC NGG C + D    + C C   F G  C   VD  V G+ C NG  C     + +C+C  GF G  C+I   Y     C+DI  C N G C+L    +  CVC +G++G  CE  +  C  N C++   C D    Y C C  G+ G  CE      T + TL        S  D  +QS+++ E+  N     +V S  VC   +                  C +   C   + N N  + C C+ G+ G  C+ + S  F   S          ++      SL     SS   I Y+   +      +   HL  S N LG+K++  +   GY    M  +S  H L + +   +  T+++ G  +  ++ +    N +  +   +      +  L+  + +F  I     FIGCM  + ++   I F+    ++I++  GC +
Sbjct:  255 CSLVNGTSRTTQCECPSGFMGADCKELQARPCDREPCLNGGHCVD-DGQNLFTCFCLPSFTGIYCGEPVDCLVNGSDCKNGGKCVFALAATTCQCPEGFNGSNCEISNSYRSHPTCSDIR-CLNGGSCKLDAEGEPFCVCEEGFDGPFCEPKSG-CTINPCQNGGTCQDADGQYFCHCTSGFGGVHCETVDEPSTPIPTLGTFPSFTTSGIDGFSQSKISCEDCVNSSNCLDVESGPVCICDDGYFGQKCDQKHDKCSKVSCPSGQTCSQVNDNVNITAQCGCEIGHFGQQCEMVTSATFSAKSL---------YIHQSSKFSLG---TSSFENIAYE--LEFSFRTTVENTHLASSENILGEKILSIQLLSGYLVFNMTGNSLEHLLPMRVSDAQWYTVFVKGEDN-KIQIEVSTENGFSLVQKSV------NGQLEVFLTRFGKISGTHHFIGCMADVRVDGDLIIFADN-KRAIDIRKGCTR 714          

HSP 4 Score: 96.2857 bits (238), Expect = 1.634e-19
Identity = 83/299 (27.76%), Postives = 128/299 (42.81%), Query Frame = 1
Query: 2797 NGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATC-NDLNNSYSCKCQSGFRGKYCDI-KFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCE-RNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCE-----------------IPAWKPTVLKTLISRRDIN-QSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNSIYFQK---NSYIEISPPS 3621
            NG   +   +  +C CP  F G+NC+  +N C    C   G C  +D     +C+C  GF G  C +  D+C G  C NG  C  + +    C+C + + G  C++ K   C D +PCQN GQC    +  ++C C  G+ G+ CE R+ ++C +  C +   CV   +   C C  GY G+FCE                  P W  TV     + R  + Q +M              C    C N+G+C   D+N      C+C+  + G  C+   S    +      I++SP +
Sbjct: 1185 NGATCVDIWNKRKCVCPAGFAGENCEDNVNDCKFVDCGKHGYC--LDGIDEAKCICNNGFHGEHCELAKDECEGVECHNGGKCVKNRSEKIVCQCGNSWMGDSCNVTKTTNCKD-SPCQNFGQCMQKTDTFFECNCMDGYSGELCEQRDVNECNHYDC-NRGHCVMTVSGPACQCEMGYTGRFCEKLLNQCSSNTCSSRGACSPVWNNTVCNCDNNWRGAHCQHQM------------DTCLDFPCNNDGVCRTNDENTFS---CECQKFFMGTRCEIEGSCLKAQCVHGECIQLSPET 1464          

HSP 5 Score: 92.0485 bits (227), Expect = 3.350e-18
Identity = 71/234 (30.34%), Postives = 99/234 (42.31%), Query Frame = 1
Query: 2869 CDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCE--RNTDDCKYNSCKHESQCVD-LHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLC 3561
            C     +C    C+N G C  +   GN +C CP GF G  C  + D+C  N C NGA C +L  SY CKC  GF GKYC+I+       + C N+GQC +   +D  C CS G++G  CE     ++C   +  H    V+      +C C  G++G  C+    +P                               C    CLN G C++  QN      C C P ++G  C
Sbjct:  122 CQSNEPSCATHTCQNNGTC--VAENGNVKCACPPGFVGDHCETDEDECKENFCQNGADCENLKGSYECKCLKGFSGKYCEIQDKKQCTSDYCHNNGQC-ISTGSDLSCKCSPGFDGAFCELKAEVNECICENPAHVCSLVNGTSRTTQCECPSGFMGADCKELQARP-------------------------------CDREPCLNGGHCVDDGQN---LFTCFCLPSFTGIYC 318          

HSP 6 Score: 88.1965 bits (217), Expect = 4.244e-17
Identity = 86/296 (29.05%), Postives = 125/296 (42.23%), Query Frame = 1
Query: 2743 DAYITAKCDSCMKRPCKH----NGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATC--NDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNS----GQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTV--LKTLISRRD-INQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSY-CKCKPGYSGHLCQYLNSIYFQ 3588
            D Y    C+      C H     G C ++T     C C   + G+ C+K +N C    C + G C  +  + N  C C   +RG  C   +D C+   C N   C  ND  N++SC+CQ  F G  C+I+         C  +    G+C  L    + C C+ G+EG  C++  D CK   C + + C +    YKC C  G+ G  CEI   +  +   K     RD IN  E   +    E  + +    +EC N   CING+      +Y C C+ G+ G  C   N    Q
Sbjct: 1319 DGYSGELCEQRDVNECNHYDCNRGHC-VMTVSGPACQCEMGYTGRFCEKLLNQCSSNTCSSRGACSPV--WNNTVCNCDNNWRGAHCQHQMDTCLDFPCNNDGVCRTND-ENTFSCECQKFFMGTRCEIE-------GSCLKAQCVHGECIQLSPETHTCSCNIGYEGDACDKKIDYCKAGPCLNGANCENKLTGYKCTCAVGFEGADCEINIDECALEFCKNGAKCRDKINDYECVCDGTGFEGRNCTT-DINECANPNNCINGECTNTLGNYKCACRNGFIGPRCSVRNPCTAQ 1602          

HSP 7 Score: 82.0333 bits (201), Expect = 3.556e-15
Identity = 75/255 (29.41%), Postives = 105/255 (41.18%), Query Frame = 1
Query: 2788 CKHNGKCSLITHHNYQCDCPYPFHGKNCD-----KRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDI---------------------------KFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDY----KCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMT--YENLENEV 3438
            CK+ GKC +       C CP  F+G NC+     +    C    C NGG CK +D  G   CVC  GF GP C      C  N C NG TC D +  Y C C SGF G +C+                              + C D   C NS  C L   +   C+C  G+ G++C++  D C   SC     C  ++++     +C C  G+ G+ CE+        K+L   +    S  T  +EN+  E+
Sbjct:  331 CKNGGKC-VFALAATTCQCPEGFNGSNCEISNSYRSHPTCSDIRCLNGGSCK-LDAEGEPFCVCEEGFDGPFCEPK-SGCTINPCQNGGTCQDADGQYFCHCTSGFGGVHCETVDEPSTPIPTLGTFPSFTTSGIDGFSQSKISCED---CVNSSNC-LDVESGPVCICDDGYFGQKCDQKHDKCSKVSCPSGQTCSQVNDNVNITAQCGCEIGHFGQQCEMVTSATFSAKSLYIHQSSKFSLGTSSFENIAYEL 578          

HSP 8 Score: 81.6481 bits (200), Expect = 4.354e-15
Identity = 64/224 (28.57%), Postives = 97/224 (43.30%), Query Frame = 1
Query: 2905 CRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCE-RNTDDCKYNSCKHESQCVDLHND-YKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYL 3570
            C NG  C  +D +   +CVCP GF G  C  NV+DC    C     C D  +   C C +GF G++C++    C  +  C N G+C    +    C C   W G  C    T +CK + C++  QC+   +  ++C C  GY G+ CE              +RD+N+                 C +++C N G C+           C+C+ GY+G  C+ L
Sbjct: 1183 CLNGATC--VDIWNKRKCVCPAGFAGENCEDNVNDCKFVDCGKHGYCLDGIDEAKCICNNGFHGEHCELAKDECEGVE-CHNGGKCVKNRSEKIVCQCGNSWMGDSCNVTKTTNCKDSPCQNFGQCMQKTDTFFECNCMDGYSGELCE--------------QRDVNE-----------------CNHYDC-NRGHCV----MTVSGPACQCEMGYTGRFCEKL 1367          

HSP 9 Score: 63.5438 bits (153), Expect = 1.560e-9
Identity = 64/257 (24.90%), Postives = 103/257 (40.08%), Query Frame = 1
Query: 2833 QCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTIN-VDDCVGNLCVNGATCNDLNNS-YSCKCQSGFRGKYCDIKFLYCNDINPCQ----NSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCE--------IPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLC 3561
            +C C   FHG++C+   + C G  C NGG C   +      C C   + G  C +    +C  + C N   C    ++ + C C  G+ G+ C+ +     D+N C     N G C ++  +   C C  G+ G+ CE+  + C  N+C     C  + N+  C C   + G  C+         P     V +T        + +  +     E+  E  C   +C+ +G CI   Q   +   C C  GY G  C
Sbjct: 1235 KCICNNGFHGEHCELAKDECEGVECHNGGKCVK-NRSEKIVCQCGNSWMGDSCNVTKTTNCKDSPCQNFGQCMQKTDTFFECNCMDGYSGELCEQR-----DVNECNHYDCNRGHC-VMTVSGPACQCEMGYTGRFCEKLLNQCSSNTCSSRGACSPVWNNTVCNCDNNWRGAHCQHQMDTCLDFPCNNDGVCRTNDENTFSCECQKFFMGTRCEI--EGSCLKAQCV-HGECI---QLSPETHTCSCNIGYEGDAC 1478          
BLAST of slit1 vs. Ensembl Fly
Match: sli (gene:FBgn0264089 transcript:FBtr0330730)

HSP 1 Score: 738.413 bits (1905), Expect = 0.000e+0
Identity = 485/1503 (32.27%), Postives = 747/1503 (49.70%), Query Frame = 1
Query:  157 CPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQLQCINDNIFNKLGMLKYLLLEGNKIFWISEAI-SKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCAS-EDKANLENMCPSDKKVCSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQIYNFQCN-KEFESKYETHDEECVPKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLPPIYSIHEAPNLMKLDLSFNQIETLEPDVFTRSSKLREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQFHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCDGNASVYHSVDSCHETNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVLSFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNL-CVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCN-DINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNSIYF-QKNSYIEISPPSRGFLMPRGNISLNFMTNSSSGLILYQGSEQTYLVVNLYQGHLRISYNLGDKVVGPEGYSMGMVNDSRLHALHIEIVGENLTMYLNGRVHCTLKSKYLN--SNRYMALDTGIFIGGAPHKILQSAIMKFHIETDSEFIGCMESLLINRKKIDFSKYLPKSINVHPGCGKLISTVVASDLKIPSIMXXXXXXXXXXXXXXXNNVCKRPGICKNQGVCEVLPGSKKK--FRCICKNGFVGRKCKKRKSMCHGTFTESHIVDPDSQGTCKSIERFQYRICTGTCSTSRMQSDVIKNFYHKPIDPSSSRCCQPTKYSTHEIQFKCENGRVYRRAANLPKNCKCVANC 4635
            CP  C C   +V+C HR   +VP  +     RL L GN + VI ++DF +L  L++LQL++N+I +IE  +F +LV+L +L ++ N+I  +   +F     L+ L L +NQ+ C++++ F  L  L+ L L  N +  +   I   L  L+ LR+  N   C+CHL+WL+ FL+  + +    A    C +P +L    ++ L   EF+C+   + A +E  C ++   C   C C+ DGIV C  K L+++P  LP +  EL L+ N IT L     S ++ L++I L+ N +  I   A  GL QLT++ L  N +K +    F GL SL  L L  N+I C+ KD       L LL L +N + SL   TF    +++ ++L  NP  CDC + W+ DY+      T  A C  P+ +    + +L+   F+C+  E   K      EC     CP  C C    V+C+  GL  +P  I   T  L+++ N L ++         P+L+KL+L  NQ+  +EP+ F  +S ++E+ L  N++K I+N+ F  L +L+ L L +N ISC++  SF++  +L  L L  N F+C+C L W +EW R      G  R   C +P  ++D  I  L    F C    S     + C         C+                    T V CS  QLKEIP  IP +T+ELYL+ N ++ ++    ++L +L  L LS N IT L+      L  L TL++S+NKLQC+ + A   L+NL+VL L  N +S +P  +F DLK+L +IALG NP +CDC +KW + +    +++ GI+ CA PE+MK K I  +  + F+C     +  I AKC++C ++PC++  +C  +    YQC C   +HGK+C+  I+AC+G PCRN   C  ++  G + C C  G+ G +C  N+DDC+G + C N ATC D   SY C+CQ GF G++CD K  +C+ + NPC N  +C +     Y C C  G+ G  C  N DDC+ + C++   CVD  NDY+C C   Y GK+CE                  N   M Y          S C  HEC  +G+C   +  +     C+C PGY+G  C+YL SI F   NS++E+ P       P  N+++ F +   +G+++Y G +  +L V L+ G +R+SY++G+  V    YS  MV D + HA+ +  + +N T+    RV   L    +N  SN Y+ L T +F+GG P    Q A   + I   + F GCM+ + IN K +DF     +   + PGC  L       +      M     I  +       N C+R   C        +P S  +  ++C CK+G  GR C +  S C       +  + D    C+S +  +Y  C G C                      ++CC        +++  C N R Y +  ++ + C C   C
Sbjct:   73 CPRVCSCTGLNVDCSHRGLTSVPRKISADVERLELQGNNLTVIYETDFQRLTKLRMLQLTDNQIHTIERNSFQDLVSLERLDISNNVITTVGRRVFKGAQSLRSLQLDNNQITCLDEHAFKGLVELEILTLNNNNLTSLPHNIFGGLGRLRALRLSDNPFACDCHLSWLSRFLRSATRL----APYTRCQSPSQLKGQNVADLHDQEFKCSGLTEHAPME--CGAENS-CPHPCRCA-DGIVDCREKSLTSVPVTLPDDTTELRLEQNFITELPPKSFSSFRRLRRIDLSNNNISRIAHDALSGLKQLTTLVLYGNKIKDLPSGVFKGLGSLQLLLLNANEISCIRKDAFRDLHSLSLLSLYDNNIQSLANGTFDAMKSIKTVHLAKNPFICDCNLRWLADYLHKNPIETSGARCESPKRMHRRRIESLREEKFKCSWDELRMKLSG---ECRMDSDCPAMCHCEGTTVDCTGRGLKEIPRDIPLHTTELLLNDNELGRISSDGLFGRLPHLVKLELKRNQLTGIEPNAFEGASHIQELQLGENKIKEISNKMFLGLHQLKTLNLYDNQISCVMPGSFEHLNSLTSLNLASNPFNCNCHLAWFAEWLRKKSLNGGAAR---CGAPSKVRDVQIKDLPHSEFKCSSENS-----EGCLGDGYCPPSCTC-----------------TGTVVRCSRNQLKEIPRGIPAETSELYLESNEIEQIHYERIRHLRSLTRLDLSNNQITILSNYTFANLTKLSTLIISYNKLQCLQRHALSGLNNLRVLSLHGNRISMLPEGSFEDLKSLTHIALGSNPLYCDCGLKWFSDWIKLDYVEPGIARCAEPEQMKDKLILSTPSSSFVCRGRVRND-ILAKCNACFEQPCQNQAQCVALPQREYQCLCQPGYHGKHCEFMIDACYGNPCRNNATCTVLEE-GRFSCQCAPGYTGARCETNIDDCLGEIKCQNNATCIDGVESYKCECQPGFSGEFCDTKIQFCSPEFNPCANGAKC-MDHFTHYSCDCQAGFHGTNCTDNIDDCQNHMCQNGGTCVDGINDYQCRCPDDYTGKYCE----------------GHNMISMMYPQT-------SPCQNHEC-KHGVCFQPNA-QGSDYLCRCHPGYTGKWCEYLTSISFVHNNSFVELEPLR---TRPEANVTIVFSSAEQNGILMYDG-QDAHLAVELFNGRIRVSYDVGNHPVSTM-YSFEMVADGKYHAVELLAIKKNFTL----RVDRGLARSIINEGSNDYLKLTTPMFLGGLPVDPAQQAYKNWQIRNLTSFKGCMKEVWINHKLVDFGN-AQRQQKITPGCALLEGEQQEEEDDEQDFMDETPHIKEEPVDPCLENKCRRGSRC--------VPNSNARDGYQCKCKHGQRGRYCDQAASTCRKEQVREYYTEND----CRSRQPLKYAKCVGGC---------------------GNQCCAAKIVRRRKVRMVCSNNRKYIKNLDIVRKCGCTKKC 1468          

HSP 2 Score: 181.415 bits (459), Expect = 2.002e-45
Identity = 135/468 (28.85%), Postives = 219/468 (46.79%), Query Frame = 1
Query: 1504 VPKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNL--------KKLPPIY---------------SIHEAPNLMKLDLSFNQIETLEPDVFTRSSKLREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQFHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCDGNASVYHSVDSCHETNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVLSFNKLQ------------------------CIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKC 2766
            + +  CP  C+C+   V+CS+ GL S+P  I    + L +  NNL        ++L  +                S  +  +L +LD+S N I T+   VF  +  LR + L++N++ C++   FK L ELEIL L+ N ++ +  + F     L+ L L+ N F CDC L W+S + R+   +    R   CQSP  LK   +  L D+ F C G     H+   C   NS    C   +                   VDC +  L  +P  +P DTTEL L++N +  L   SF +   L+ + LS N I+ +  + L+ LK L TLVL  NK++                        CI +DAF++LH+L +L L  N++ ++    F+ +K++  + L +NPF CDCN++WL  +     ++   + C SP++M  + I   +   F CS +     ++ +C
Sbjct:   68 ITEARCPRVCSCTGLNVDCSHRGLTSVPRKISADVERLELQGNNLTVIYETDFQRLTKLRMLQLTDNQIHTIERNSFQDLVSLERLDISNNVITTVGRRVFKGAQSLRSLQLDNNQITCLDEHAFKGLVELEILTLNNNNLTSLPHNIFGGLGRLRALRLSDNPFACDCHLSWLSRFLRSATRLAPYTR---CQSPSQLKGQNVADLHDQEFKCSGLTE--HAPMECGAENSCPHPCRCAD-----------------GIVDCREKSLTSVPVTLPDDTTELRLEQNFITELPPKSFSSFRRLRRIDLSNNNISRIAHDALSGLKQLTTLVLYGNKIKDLPSGVFKGLGSLQLLLLNANEISCIRKDAFRDLHSLSLLSLYDNNIQSLANGTFDAMKSIKTVHLAKNPFICDCNLRWLADYLHKNPIETSGARCESPKRMHRRRIESLREEKFKCSWDELRMKLSGEC 513          
BLAST of slit1 vs. Ensembl Fly
Match: sli (gene:FBgn0264089 transcript:FBtr0330729)

HSP 1 Score: 731.865 bits (1888), Expect = 0.000e+0
Identity = 483/1510 (31.99%), Postives = 749/1510 (49.60%), Query Frame = 1
Query:  157 CPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQLQCINDNIFNKLGMLKYLLLEGNKIFWISEAI-SKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCAS-EDKANLENMCPSDKKVCSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQIYNFQCN-KEFESKYETHDEECVPKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLPPIYSIHEAPNLMKLDLSFNQIETLEPDVFTRSSKLREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQFHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCDGNASVYHSVDSCHETNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVLSFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNL-CVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCN-DINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNSIYF-QKNSYIEISPPSRGFLMPRGNISLNFMTNSSSGLILYQGSEQTYLVVNLYQGHLRISYNLGDKVVGPEGYSMGMVNDSRLHALHIEIVGENLTMYLNGRVHCTLKSKYLN--SNRYMALDTGIFIGGAPHKILQSAIMKFHIETDSEFIGCMESLLINRKKIDFSKYLPKSINVHPGCGKLISTVVASDLKIPSIMXXXXXXXXXXXXXXXNNVCKRPGICKNQGVCEVLPGSKKK--FRCICKNGFVGRKCKKRKS-------MCHGTFTESHIVDPDSQGTCKSIERFQYRICTGTCSTSRMQSDVIKNFYHKPIDPSSSRCCQPTKYSTHEIQFKCENGRVYRRAANLPKNCKCVANC 4635
            CP  C C   +V+C HR   +VP  +     RL L GN + VI ++DF +L  L++LQL++N+I +IE  +F +LV+L +L ++ N+I  +   +F     L+ L L +NQ+ C++++ F  L  L+ L L  N +  +   I   L  L+ LR+  N   C+CHL+WL+ FL+  + +    A    C +P +L    ++ L   EF+C+   + A +E  C ++   C   C C+ DGIV C  K L+++P  LP +  EL L+ N IT L     S ++ L++I L+ N +  I   A  GL QLT++ L  N +K +    F GL SL  L L  N+I C+ KD       L LL L +N + SL   TF    +++ ++L  NP  CDC + W+ DY+      T  A C  P+ +    + +L+   F+C+  E   K      EC     CP  C C    V+C+  GL  +P  I   T  L+++ N L ++         P+L+KL+L  NQ+  +EP+ F  +S ++E+ L  N++K I+N+ F  L +L+ L L +N ISC++  SF++  +L  L L  N F+C+C L W +EW R      G  R   C +P  ++D  I  L    F C    S     + C         C+                    T V CS  QLKEIP  IP +T+ELYL+ N ++ ++    ++L +L  L LS N IT L+      L  L TL++S+NKLQC+ + A   L+NL+VL L  N +S +P  +F DLK+L +IALG NP +CDC +KW + +    +++ GI+ CA PE+MK K I  +  + F+C     +  I AKC++C ++PC++  +C  +    YQC C   +HGK+C+  I+AC+G PCRN   C  ++  G + C C  G+ G +C  N+DDC+G + C N ATC D   SY C+CQ GF G++CD K  +C+ + NPC N  +C +     Y C C  G+ G  C  N DDC+ + C++   CVD  NDY+C C   Y GK+CE                  N   M Y          S C  HEC  +G+C   +  +     C+C PGY+G  C+YL SI F   NS++E+ P       P  N+++ F +   +G+++Y G +  +L V L+ G +R+SY++G+  V    YS  MV D + HA+ +  + +N T+    RV   L    +N  SN Y+ L T +F+GG P    Q A   + I   + F GCM+ + IN K +DF     +   + PGC  L       +      M     I  +       N C+R   C        +P S  +  ++C CK+G  GR C + +            T  +  + +  ++  C+S +  +Y  C G C                      ++CC        +++  C N R Y +  ++ + C C   C
Sbjct:   73 CPRVCSCTGLNVDCSHRGLTSVPRKISADVERLELQGNNLTVIYETDFQRLTKLRMLQLTDNQIHTIERNSFQDLVSLERLDISNNVITTVGRRVFKGAQSLRSLQLDNNQITCLDEHAFKGLVELEILTLNNNNLTSLPHNIFGGLGRLRALRLSDNPFACDCHLSWLSRFLRSATRL----APYTRCQSPSQLKGQNVADLHDQEFKCSGLTEHAPME--CGAENS-CPHPCRCA-DGIVDCREKSLTSVPVTLPDDTTELRLEQNFITELPPKSFSSFRRLRRIDLSNNNISRIAHDALSGLKQLTTLVLYGNKIKDLPSGVFKGLGSLQLLLLNANEISCIRKDAFRDLHSLSLLSLYDNNIQSLANGTFDAMKSIKTVHLAKNPFICDCNLRWLADYLHKNPIETSGARCESPKRMHRRRIESLREEKFKCSWDELRMKLSG---ECRMDSDCPAMCHCEGTTVDCTGRGLKEIPRDIPLHTTELLLNDNELGRISSDGLFGRLPHLVKLELKRNQLTGIEPNAFEGASHIQELQLGENKIKEISNKMFLGLHQLKTLNLYDNQISCVMPGSFEHLNSLTSLNLASNPFNCNCHLAWFAEWLRKKSLNGGAAR---CGAPSKVRDVQIKDLPHSEFKCSSENS-----EGCLGDGYCPPSCTC-----------------TGTVVRCSRNQLKEIPRGIPAETSELYLESNEIEQIHYERIRHLRSLTRLDLSNNQITILSNYTFANLTKLSTLIISYNKLQCLQRHALSGLNNLRVLSLHGNRISMLPEGSFEDLKSLTHIALGSNPLYCDCGLKWFSDWIKLDYVEPGIARCAEPEQMKDKLILSTPSSSFVCRGRVRND-ILAKCNACFEQPCQNQAQCVALPQREYQCLCQPGYHGKHCEFMIDACYGNPCRNNATCTVLEE-GRFSCQCAPGYTGARCETNIDDCLGEIKCQNNATCIDGVESYKCECQPGFSGEFCDTKIQFCSPEFNPCANGAKC-MDHFTHYSCDCQAGFHGTNCTDNIDDCQNHMCQNGGTCVDGINDYQCRCPDDYTGKYCE----------------GHNMISMMYPQT-------SPCQNHEC-KHGVCFQPNA-QGSDYLCRCHPGYTGKWCEYLTSISFVHNNSFVELEPLR---TRPEANVTIVFSSAEQNGILMYDG-QDAHLAVELFNGRIRVSYDVGNHPVSTM-YSFEMVADGKYHAVELLAIKKNFTL----RVDRGLARSIINEGSNDYLKLTTPMFLGGLPVDPAQQAYKNWQIRNLTSFKGCMKEVWINHKLVDFGN-AQRQQKITPGCALLEGEQQEEEDDEQDFMDETPHIKEEPVDPCLENKCRRGSRC--------VPNSNARDGYQCKCKHGQRGRYCDQGEGSTEPPTVTAASTCRKEQVREYYTENDCRSRQPLKYAKCVGGC---------------------GNQCCAAKIVRRRKVRMVCSNNRKYIKNLDIVRKCGCTKKC 1479          

HSP 2 Score: 181.03 bits (458), Expect = 2.315e-45
Identity = 135/468 (28.85%), Postives = 219/468 (46.79%), Query Frame = 1
Query: 1504 VPKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNL--------KKLPPIY---------------SIHEAPNLMKLDLSFNQIETLEPDVFTRSSKLREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQFHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCDGNASVYHSVDSCHETNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVLSFNKLQ------------------------CIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKC 2766
            + +  CP  C+C+   V+CS+ GL S+P  I    + L +  NNL        ++L  +                S  +  +L +LD+S N I T+   VF  +  LR + L++N++ C++   FK L ELEIL L+ N ++ +  + F     L+ L L+ N F CDC L W+S + R+   +    R   CQSP  LK   +  L D+ F C G     H+   C   NS    C   +                   VDC +  L  +P  +P DTTEL L++N +  L   SF +   L+ + LS N I+ +  + L+ LK L TLVL  NK++                        CI +DAF++LH+L +L L  N++ ++    F+ +K++  + L +NPF CDCN++WL  +     ++   + C SP++M  + I   +   F CS +     ++ +C
Sbjct:   68 ITEARCPRVCSCTGLNVDCSHRGLTSVPRKISADVERLELQGNNLTVIYETDFQRLTKLRMLQLTDNQIHTIERNSFQDLVSLERLDISNNVITTVGRRVFKGAQSLRSLQLDNNQITCLDEHAFKGLVELEILTLNNNNLTSLPHNIFGGLGRLRALRLSDNPFACDCHLSWLSRFLRSATRLAPYTR---CQSPSQLKGQNVADLHDQEFKCSG--LTEHAPMECGAENSCPHPCRCAD-----------------GIVDCREKSLTSVPVTLPDDTTELRLEQNFITELPPKSFSSFRRLRRIDLSNNNISRIAHDALSGLKQLTTLVLYGNKIKDLPSGVFKGLGSLQLLLLNANEISCIRKDAFRDLHSLSLLSLYDNNIQSLANGTFDAMKSIKTVHLAKNPFICDCNLRWLADYLHKNPIETSGARCESPKRMHRRRIESLREEKFKCSWDELRMKLSGEC 513          
BLAST of slit1 vs. Ensembl Fly
Match: sli (gene:FBgn0264089 transcript:FBtr0330732)

HSP 1 Score: 731.865 bits (1888), Expect = 0.000e+0
Identity = 483/1510 (31.99%), Postives = 749/1510 (49.60%), Query Frame = 1
Query:  157 CPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQLQCINDNIFNKLGMLKYLLLEGNKIFWISEAI-SKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCAS-EDKANLENMCPSDKKVCSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQIYNFQCN-KEFESKYETHDEECVPKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLPPIYSIHEAPNLMKLDLSFNQIETLEPDVFTRSSKLREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQFHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCDGNASVYHSVDSCHETNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVLSFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNL-CVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCN-DINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNSIYF-QKNSYIEISPPSRGFLMPRGNISLNFMTNSSSGLILYQGSEQTYLVVNLYQGHLRISYNLGDKVVGPEGYSMGMVNDSRLHALHIEIVGENLTMYLNGRVHCTLKSKYLN--SNRYMALDTGIFIGGAPHKILQSAIMKFHIETDSEFIGCMESLLINRKKIDFSKYLPKSINVHPGCGKLISTVVASDLKIPSIMXXXXXXXXXXXXXXXNNVCKRPGICKNQGVCEVLPGSKKK--FRCICKNGFVGRKCKKRKS-------MCHGTFTESHIVDPDSQGTCKSIERFQYRICTGTCSTSRMQSDVIKNFYHKPIDPSSSRCCQPTKYSTHEIQFKCENGRVYRRAANLPKNCKCVANC 4635
            CP  C C   +V+C HR   +VP  +     RL L GN + VI ++DF +L  L++LQL++N+I +IE  +F +LV+L +L ++ N+I  +   +F     L+ L L +NQ+ C++++ F  L  L+ L L  N +  +   I   L  L+ LR+  N   C+CHL+WL+ FL+  + +    A    C +P +L    ++ L   EF+C+   + A +E  C ++   C   C C+ DGIV C  K L+++P  LP +  EL L+ N IT L     S ++ L++I L+ N +  I   A  GL QLT++ L  N +K +    F GL SL  L L  N+I C+ KD       L LL L +N + SL   TF    +++ ++L  NP  CDC + W+ DY+      T  A C  P+ +    + +L+   F+C+  E   K      EC     CP  C C    V+C+  GL  +P  I   T  L+++ N L ++         P+L+KL+L  NQ+  +EP+ F  +S ++E+ L  N++K I+N+ F  L +L+ L L +N ISC++  SF++  +L  L L  N F+C+C L W +EW R      G  R   C +P  ++D  I  L    F C    S     + C         C+                    T V CS  QLKEIP  IP +T+ELYL+ N ++ ++    ++L +L  L LS N IT L+      L  L TL++S+NKLQC+ + A   L+NL+VL L  N +S +P  +F DLK+L +IALG NP +CDC +KW + +    +++ GI+ CA PE+MK K I  +  + F+C     +  I AKC++C ++PC++  +C  +    YQC C   +HGK+C+  I+AC+G PCRN   C  ++  G + C C  G+ G +C  N+DDC+G + C N ATC D   SY C+CQ GF G++CD K  +C+ + NPC N  +C +     Y C C  G+ G  C  N DDC+ + C++   CVD  NDY+C C   Y GK+CE                  N   M Y          S C  HEC  +G+C   +  +     C+C PGY+G  C+YL SI F   NS++E+ P       P  N+++ F +   +G+++Y G +  +L V L+ G +R+SY++G+  V    YS  MV D + HA+ +  + +N T+    RV   L    +N  SN Y+ L T +F+GG P    Q A   + I   + F GCM+ + IN K +DF     +   + PGC  L       +      M     I  +       N C+R   C        +P S  +  ++C CK+G  GR C + +            T  +  + +  ++  C+S +  +Y  C G C                      ++CC        +++  C N R Y +  ++ + C C   C
Sbjct:   73 CPRVCSCTGLNVDCSHRGLTSVPRKISADVERLELQGNNLTVIYETDFQRLTKLRMLQLTDNQIHTIERNSFQDLVSLERLDISNNVITTVGRRVFKGAQSLRSLQLDNNQITCLDEHAFKGLVELEILTLNNNNLTSLPHNIFGGLGRLRALRLSDNPFACDCHLSWLSRFLRSATRL----APYTRCQSPSQLKGQNVADLHDQEFKCSGLTEHAPME--CGAENS-CPHPCRCA-DGIVDCREKSLTSVPVTLPDDTTELRLEQNFITELPPKSFSSFRRLRRIDLSNNNISRIAHDALSGLKQLTTLVLYGNKIKDLPSGVFKGLGSLQLLLLNANEISCIRKDAFRDLHSLSLLSLYDNNIQSLANGTFDAMKSIKTVHLAKNPFICDCNLRWLADYLHKNPIETSGARCESPKRMHRRRIESLREEKFKCSWDELRMKLSG---ECRMDSDCPAMCHCEGTTVDCTGRGLKEIPRDIPLHTTELLLNDNELGRISSDGLFGRLPHLVKLELKRNQLTGIEPNAFEGASHIQELQLGENKIKEISNKMFLGLHQLKTLNLYDNQISCVMPGSFEHLNSLTSLNLASNPFNCNCHLAWFAEWLRKKSLNGGAAR---CGAPSKVRDVQIKDLPHSEFKCSSENS-----EGCLGDGYCPPSCTC-----------------TGTVVRCSRNQLKEIPRGIPAETSELYLESNEIEQIHYERIRHLRSLTRLDLSNNQITILSNYTFANLTKLSTLIISYNKLQCLQRHALSGLNNLRVLSLHGNRISMLPEGSFEDLKSLTHIALGSNPLYCDCGLKWFSDWIKLDYVEPGIARCAEPEQMKDKLILSTPSSSFVCRGRVRND-ILAKCNACFEQPCQNQAQCVALPQREYQCLCQPGYHGKHCEFMIDACYGNPCRNNATCTVLEE-GRFSCQCAPGYTGARCETNIDDCLGEIKCQNNATCIDGVESYKCECQPGFSGEFCDTKIQFCSPEFNPCANGAKC-MDHFTHYSCDCQAGFHGTNCTDNIDDCQNHMCQNGGTCVDGINDYQCRCPDDYTGKYCE----------------GHNMISMMYPQT-------SPCQNHEC-KHGVCFQPNA-QGSDYLCRCHPGYTGKWCEYLTSISFVHNNSFVELEPLR---TRPEANVTIVFSSAEQNGILMYDG-QDAHLAVELFNGRIRVSYDVGNHPVSTM-YSFEMVADGKYHAVELLAIKKNFTL----RVDRGLARSIINEGSNDYLKLTTPMFLGGLPVDPAQQAYKNWQIRNLTSFKGCMKEVWINHKLVDFGN-AQRQQKITPGCALLEGEQQEEEDDEQDFMDETPHIKEEPVDPCLENKCRRGSRC--------VPNSNARDGYQCKCKHGQRGRYCDQGEGSTEPPTVTAASTCRKEQVREYYTENDCRSRQPLKYAKCVGGC---------------------GNQCCAAKIVRRRKVRMVCSNNRKYIKNLDIVRKCGCTKKC 1479          

HSP 2 Score: 181.03 bits (458), Expect = 2.315e-45
Identity = 135/468 (28.85%), Postives = 219/468 (46.79%), Query Frame = 1
Query: 1504 VPKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNL--------KKLPPIY---------------SIHEAPNLMKLDLSFNQIETLEPDVFTRSSKLREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQFHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCDGNASVYHSVDSCHETNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVLSFNKLQ------------------------CIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKC 2766
            + +  CP  C+C+   V+CS+ GL S+P  I    + L +  NNL        ++L  +                S  +  +L +LD+S N I T+   VF  +  LR + L++N++ C++   FK L ELEIL L+ N ++ +  + F     L+ L L+ N F CDC L W+S + R+   +    R   CQSP  LK   +  L D+ F C G     H+   C   NS    C   +                   VDC +  L  +P  +P DTTEL L++N +  L   SF +   L+ + LS N I+ +  + L+ LK L TLVL  NK++                        CI +DAF++LH+L +L L  N++ ++    F+ +K++  + L +NPF CDCN++WL  +     ++   + C SP++M  + I   +   F CS +     ++ +C
Sbjct:   68 ITEARCPRVCSCTGLNVDCSHRGLTSVPRKISADVERLELQGNNLTVIYETDFQRLTKLRMLQLTDNQIHTIERNSFQDLVSLERLDISNNVITTVGRRVFKGAQSLRSLQLDNNQITCLDEHAFKGLVELEILTLNNNNLTSLPHNIFGGLGRLRALRLSDNPFACDCHLSWLSRFLRSATRLAPYTR---CQSPSQLKGQNVADLHDQEFKCSG--LTEHAPMECGAENSCPHPCRCAD-----------------GIVDCREKSLTSVPVTLPDDTTELRLEQNFITELPPKSFSSFRRLRRIDLSNNNISRIAHDALSGLKQLTTLVLYGNKIKDLPSGVFKGLGSLQLLLLNANEISCIRKDAFRDLHSLSLLSLYDNNIQSLANGTFDAMKSIKTVHLAKNPFICDCNLRWLADYLHKNPIETSGARCESPKRMHRRRIESLREEKFKCSWDELRMKLSGEC 513          
BLAST of slit1 vs. Ensembl Fly
Match: sli (gene:FBgn0264089 transcript:FBtr0330734)

HSP 1 Score: 726.857 bits (1875), Expect = 0.000e+0
Identity = 486/1535 (31.66%), Postives = 751/1535 (48.93%), Query Frame = 1
Query:  157 CPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQLQCINDNIFNKLGMLKYLLLEGNKIFWISEAISK-------------------------LKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCAS-EDKANLENMCPSDKKVCSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQIYNFQCN-KEFESKYETHDEECVPKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLPPIYSIHEAPNLMKLDLSFNQIETLEPDVFTRSSKLREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQFHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCDGNASVYHSVDSCHETNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVLSFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNL-CVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCN-DINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSY-CKCKPGYSGHLCQYLNSIYF-QKNSYIEISPPSRGFLMPRGNISLNFMTNSSSGLILYQGSEQTYLVVNLYQGHLRISYNLGDKVVGPEGYSMGMVNDSRLHALHIEIVGENLTMYLNGRVHCTLKSKYLN--SNRYMALDTGIFIGGAPHKILQSAIMKFHIETDSEFIGCMESLLINRKKIDFSKYLPKSINVHPGCGKLISTVVASDLKIPSIMXXXXXXXXXXXXXXXNNVCKRPGICKNQGVCEVLPGSKKK--FRCICKNGFVGRKCKKRKS-------MCHGTFTESHIVDPDSQGTCKSIERFQYRICTGTCSTSRMQSDVIKNFYHKPIDPSSSRCCQPTKYSTHEIQFKCENGRVYRRAANLPKNCKCVANC 4635
            CP  C C   +V+C HR   +VP  +     RL L GN + VI ++DF +L  L++LQL++N+I +IE  +F +LV+L +LRLN N ++ +P++  +    L +LD+ +N +  +   +F     L+ L L+ N+I  + E   K                         L  L+ LR+  N   C+CHL+WL+ FL+  + +    A    C +P +L    ++ L   EF+C+   + A +E  C ++   C   C C+ DGIV C  K L+++P  LP +  EL L+ N IT L     S ++ L++I L+ N +  I   A  GL QLT++ L  N +K +    F GL SL  L L  N+I C+ KD       L LL L +N + SL   TF    +++ ++L  NP  CDC + W+ DY+      T  A C  P+ +    + +L+   F+C+  E   K      EC     CP  C C    V+C+  GL  +P  I   T  L+++ N L ++         P+L+KL+L  NQ+  +EP+ F  +S ++E+ L  N++K I+N+ F  L +L+ L L +N ISC++  SF++  +L  L L  N F+C+C L W +EW R      G  R   C +P  ++D  I  L    F C    S     + C         C+                    T V CS  QLKEIP  IP +T+ELYL+ N ++ ++    ++L +L  L LS N IT L+      L  L TL++S+NKLQC+ + A   L+NL+VL L  N +S +P  +F DLK+L +IALG NP +CDC +KW + +    +++ GI+ CA PE+MK K I  +  + F+C     +  I AKC++C ++PC++  +C  +    YQC C   +HGK+C+  I+AC+G PCRN   C  ++  G + C C  G+ G +C  N+DDC+G + C N ATC D   SY C+CQ GF G++CD K  +C+ + NPC N  +C +     Y C C  G+ G  C  N DDC+ + C++   CVD  NDY+C C   Y GK+CE                  N   M Y          S C  HEC  +G+C     N     Y C+C PGY+G  C+YL SI F   NS++E+ P       P  N+++ F +   +G+++Y G +  +L V L+ G +R+SY++G+  V    YS  MV D + HA+ +  + +N T+    RV   L    +N  SN Y+ L T +F+GG P    Q A   + I   + F GCM+ + IN K +DF     +   + PGC  L       +      M     I  +       N C+R   C        +P S  +  ++C CK+G  GR C + +            T  +  + +  ++  C+S +  +Y  C G C                      ++CC        +++  C N R Y +  ++ + C C   C
Sbjct:   73 CPRVCSCTGLNVDCSHRGLTSVPRKISADVERLELQGNNLTVIYETDFQRLTKLRMLQLTDNQIHTIERNSFQDLVSLERLRLNNNRLKAIPENFVTSSASLLRLDISNNVITTVGRRVFKGAQSLRSLQLDNNQITCLDEHAFKGLVELEILTLNNNNLTSLPHNIFGGLGRLRALRLSDNPFACDCHLSWLSRFLRSATRL----APYTRCQSPSQLKGQNVADLHDQEFKCSGLTEHAPME--CGAENS-CPHPCRCA-DGIVDCREKSLTSVPVTLPDDTTELRLEQNFITELPPKSFSSFRRLRRIDLSNNNISRIAHDALSGLKQLTTLVLYGNKIKDLPSGVFKGLGSLQLLLLNANEISCIRKDAFRDLHSLSLLSLYDNNIQSLANGTFDAMKSIKTVHLAKNPFICDCNLRWLADYLHKNPIETSGARCESPKRMHRRRIESLREEKFKCSWDELRMKLSG---ECRMDSDCPAMCHCEGTTVDCTGRGLKEIPRDIPLHTTELLLNDNELGRISSDGLFGRLPHLVKLELKRNQLTGIEPNAFEGASHIQELQLGENKIKEISNKMFLGLHQLKTLNLYDNQISCVMPGSFEHLNSLTSLNLASNPFNCNCHLAWFAEWLRKKSLNGGAAR---CGAPSKVRDVQIKDLPHSEFKCSSENS-----EGCLGDGYCPPSCTC-----------------TGTVVRCSRNQLKEIPRGIPAETSELYLESNEIEQIHYERIRHLRSLTRLDLSNNQITILSNYTFANLTKLSTLIISYNKLQCLQRHALSGLNNLRVLSLHGNRISMLPEGSFEDLKSLTHIALGSNPLYCDCGLKWFSDWIKLDYVEPGIARCAEPEQMKDKLILSTPSSSFVCRGRVRND-ILAKCNACFEQPCQNQAQCVALPQREYQCLCQPGYHGKHCEFMIDACYGNPCRNNATCTVLEE-GRFSCQCAPGYTGARCETNIDDCLGEIKCQNNATCIDGVESYKCECQPGFSGEFCDTKIQFCSPEFNPCANGAKC-MDHFTHYSCDCQAGFHGTNCTDNIDDCQNHMCQNGGTCVDGINDYQCRCPDDYTGKYCE----------------GHNMISMMYPQT-------SPCQNHEC-KHGVCFQ--PNAQGSDYLCRCHPGYTGKWCEYLTSISFVHNNSFVELEPLR---TRPEANVTIVFSSAEQNGILMYDG-QDAHLAVELFNGRIRVSYDVGNHPVSTM-YSFEMVADGKYHAVELLAIKKNFTL----RVDRGLARSIINEGSNDYLKLTTPMFLGGLPVDPAQQAYKNWQIRNLTSFKGCMKEVWINHKLVDFGN-AQRQQKITPGCALLEGEQQEEEDDEQDFMDETPHIKEEPVDPCLENKCRRGSRC--------VPNSNARDGYQCKCKHGQRGRYCDQGEGSTEPPTVTAASTCRKEQVREYYTENDCRSRQPLKYAKCVGGC---------------------GNQCCAAKIVRRRKVRMVCSNNRKYIKNLDIVRKCGCTKKC 1503          

HSP 2 Score: 174.096 bits (440), Expect = 5.066e-43
Identity = 135/492 (27.44%), Postives = 218/492 (44.31%), Query Frame = 1
Query: 1504 VPKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLK-----------------------------------------------KLPPIYSIHEAPNLMKLDLSFNQIETLEPDVFTRSSKLREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQFHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCDGNASVYHSVDSCHETNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVLSFNKLQ------------------------CIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKC 2766
            + +  CP  C+C+   V+CS+ GL S+P  I    + L +  NNL                                                K  P   +  + +L++LD+S N I T+   VF  +  LR + L++N++ C++   FK L ELEIL L+ N ++ +  + F     L+ L L+ N F CDC L W+S + R+   +    R   CQSP  LK   +  L D+ F C G     H+   C   NS    C   +                   VDC +  L  +P  +P DTTEL L++N +  L   SF +   L+ + LS N I+ +  + L+ LK L TLVL  NK++                        CI +DAF++LH+L +L L  N++ ++    F+ +K++  + L +NPF CDCN++WL  +     ++   + C SP++M  + I   +   F CS +     ++ +C
Sbjct:   68 ITEARCPRVCSCTGLNVDCSHRGLTSVPRKISADVERLELQGNNLTVIYETDFQRLTKLRMLQLTDNQIHTIERNSFQDLVSLERLRLNNNRLKAIPENFVTSSASLLRLDISNNVITTVGRRVFKGAQSLRSLQLDNNQITCLDEHAFKGLVELEILTLNNNNLTSLPHNIFGGLGRLRALRLSDNPFACDCHLSWLSRFLRSATRLAPYTR---CQSPSQLKGQNVADLHDQEFKCSG--LTEHAPMECGAENSCPHPCRCAD-----------------GIVDCREKSLTSVPVTLPDDTTELRLEQNFITELPPKSFSSFRRLRRIDLSNNNISRIAHDALSGLKQLTTLVLYGNKIKDLPSGVFKGLGSLQLLLLNANEISCIRKDAFRDLHSLSLLSLYDNNIQSLANGTFDAMKSIKTVHLAKNPFICDCNLRWLADYLHKNPIETSGARCESPKRMHRRRIESLREEKFKCSWDELRMKLSGEC 537          
BLAST of slit1 vs. Ensembl Fly
Match: sli (gene:FBgn0264089 transcript:FBtr0330733)

HSP 1 Score: 726.087 bits (1873), Expect = 0.000e+0
Identity = 484/1534 (31.55%), Postives = 751/1534 (48.96%), Query Frame = 1
Query:  157 CPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQLQCINDNIFNKLGMLKYLLLEGNKIFWISEAISK-------------------------LKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCAS-EDKANLENMCPSDKKVCSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQIYNFQCN-KEFESKYETHDEECVPKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLPPIYSIHEAPNLMKLDLSFNQIETLEPDVFTRSSKLREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQFHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCDGNASVYHSVDSCHETNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVLSFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNL-CVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCN-DINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNSIYF-QKNSYIEISPPSRGFLMPRGNISLNFMTNSSSGLILYQGSEQTYLVVNLYQGHLRISYNLGDKVVGPEGYSMGMVNDSRLHALHIEIVGENLTMYLNGRVHCTLKSKYLN--SNRYMALDTGIFIGGAPHKILQSAIMKFHIETDSEFIGCMESLLINRKKIDFSKYLPKSINVHPGCGKLISTVVASDLKIPSIMXXXXXXXXXXXXXXXNNVCKRPGICKNQGVCEVLPGSKKK--FRCICKNGFVGRKCKKRKS-------MCHGTFTESHIVDPDSQGTCKSIERFQYRICTGTCSTSRMQSDVIKNFYHKPIDPSSSRCCQPTKYSTHEIQFKCENGRVYRRAANLPKNCKCVANC 4635
            CP  C C   +V+C HR   +VP  +     RL L GN + VI ++DF +L  L++LQL++N+I +IE  +F +LV+L +LRLN N ++ +P++  +    L +LD+ +N +  +   +F     L+ L L+ N+I  + E   K                         L  L+ LR+  N   C+CHL+WL+ FL+  + +    A    C +P +L    ++ L   EF+C+   + A +E  C ++   C   C C+ DGIV C  K L+++P  LP +  EL L+ N IT L     S ++ L++I L+ N +  I   A  GL QLT++ L  N +K +    F GL SL  L L  N+I C+ KD       L LL L +N + SL   TF    +++ ++L  NP  CDC + W+ DY+      T  A C  P+ +    + +L+   F+C+  E   K      EC     CP  C C    V+C+  GL  +P  I   T  L+++ N L ++         P+L+KL+L  NQ+  +EP+ F  +S ++E+ L  N++K I+N+ F  L +L+ L L +N ISC++  SF++  +L  L L  N F+C+C L W +EW R      G  R   C +P  ++D  I  L    F C    S     + C         C+                    T V CS  QLKEIP  IP +T+ELYL+ N ++ ++    ++L +L  L LS N IT L+      L  L TL++S+NKLQC+ + A   L+NL+VL L  N +S +P  +F DLK+L +IALG NP +CDC +KW + +    +++ GI+ CA PE+MK K I  +  + F+C     +  I AKC++C ++PC++  +C  +    YQC C   +HGK+C+  I+AC+G PCRN   C  ++  G + C C  G+ G +C  N+DDC+G + C N ATC D   SY C+CQ GF G++CD K  +C+ + NPC N  +C +     Y C C  G+ G  C  N DDC+ + C++   CVD  NDY+C C   Y GK+CE                  N   M Y          S C  HEC  +G+C   +  +     C+C PGY+G  C+YL SI F   NS++E+ P       P  N+++ F +   +G+++Y G +  +L V L+ G +R+SY++G+  V    YS  MV D + HA+ +  + +N T+    RV   L    +N  SN Y+ L T +F+GG P    Q A   + I   + F GCM+ + IN K +DF     +   + PGC  L       +      M     I  +       N C+R   C        +P S  +  ++C CK+G  GR C + +            T  +  + +  ++  C+S +  +Y  C G C                      ++CC        +++  C N R Y +  ++ + C C   C
Sbjct:   73 CPRVCSCTGLNVDCSHRGLTSVPRKISADVERLELQGNNLTVIYETDFQRLTKLRMLQLTDNQIHTIERNSFQDLVSLERLRLNNNRLKAIPENFVTSSASLLRLDISNNVITTVGRRVFKGAQSLRSLQLDNNQITCLDEHAFKGLVELEILTLNNNNLTSLPHNIFGGLGRLRALRLSDNPFACDCHLSWLSRFLRSATRL----APYTRCQSPSQLKGQNVADLHDQEFKCSGLTEHAPME--CGAENS-CPHPCRCA-DGIVDCREKSLTSVPVTLPDDTTELRLEQNFITELPPKSFSSFRRLRRIDLSNNNISRIAHDALSGLKQLTTLVLYGNKIKDLPSGVFKGLGSLQLLLLNANEISCIRKDAFRDLHSLSLLSLYDNNIQSLANGTFDAMKSIKTVHLAKNPFICDCNLRWLADYLHKNPIETSGARCESPKRMHRRRIESLREEKFKCSWDELRMKLSG---ECRMDSDCPAMCHCEGTTVDCTGRGLKEIPRDIPLHTTELLLNDNELGRISSDGLFGRLPHLVKLELKRNQLTGIEPNAFEGASHIQELQLGENKIKEISNKMFLGLHQLKTLNLYDNQISCVMPGSFEHLNSLTSLNLASNPFNCNCHLAWFAEWLRKKSLNGGAAR---CGAPSKVRDVQIKDLPHSEFKCSSENS-----EGCLGDGYCPPSCTC-----------------TGTVVRCSRNQLKEIPRGIPAETSELYLESNEIEQIHYERIRHLRSLTRLDLSNNQITILSNYTFANLTKLSTLIISYNKLQCLQRHALSGLNNLRVLSLHGNRISMLPEGSFEDLKSLTHIALGSNPLYCDCGLKWFSDWIKLDYVEPGIARCAEPEQMKDKLILSTPSSSFVCRGRVRND-ILAKCNACFEQPCQNQAQCVALPQREYQCLCQPGYHGKHCEFMIDACYGNPCRNNATCTVLEE-GRFSCQCAPGYTGARCETNIDDCLGEIKCQNNATCIDGVESYKCECQPGFSGEFCDTKIQFCSPEFNPCANGAKC-MDHFTHYSCDCQAGFHGTNCTDNIDDCQNHMCQNGGTCVDGINDYQCRCPDDYTGKYCE----------------GHNMISMMYPQT-------SPCQNHEC-KHGVCFQPNA-QGSDYLCRCHPGYTGKWCEYLTSISFVHNNSFVELEPLR---TRPEANVTIVFSSAEQNGILMYDG-QDAHLAVELFNGRIRVSYDVGNHPVSTM-YSFEMVADGKYHAVELLAIKKNFTL----RVDRGLARSIINEGSNDYLKLTTPMFLGGLPVDPAQQAYKNWQIRNLTSFKGCMKEVWINHKLVDFGN-AQRQQKITPGCALLEGEQQEEEDDEQDFMDETPHIKEEPVDPCLENKCRRGSRC--------VPNSNARDGYQCKCKHGQRGRYCDQGEGSTEPPTVTAASTCRKEQVREYYTENDCRSRQPLKYAKCVGGC---------------------GNQCCAAKIVRRRKVRMVCSNNRKYIKNLDIVRKCGCTKKC 1503          

HSP 2 Score: 175.252 bits (443), Expect = 1.338e-43
Identity = 135/492 (27.44%), Postives = 218/492 (44.31%), Query Frame = 1
Query: 1504 VPKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLK-----------------------------------------------KLPPIYSIHEAPNLMKLDLSFNQIETLEPDVFTRSSKLREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQFHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCDGNASVYHSVDSCHETNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVLSFNKLQ------------------------CIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKC 2766
            + +  CP  C+C+   V+CS+ GL S+P  I    + L +  NNL                                                K  P   +  + +L++LD+S N I T+   VF  +  LR + L++N++ C++   FK L ELEIL L+ N ++ +  + F     L+ L L+ N F CDC L W+S + R+   +    R   CQSP  LK   +  L D+ F C G     H+   C   NS    C   +                   VDC +  L  +P  +P DTTEL L++N +  L   SF +   L+ + LS N I+ +  + L+ LK L TLVL  NK++                        CI +DAF++LH+L +L L  N++ ++    F+ +K++  + L +NPF CDCN++WL  +     ++   + C SP++M  + I   +   F CS +     ++ +C
Sbjct:   68 ITEARCPRVCSCTGLNVDCSHRGLTSVPRKISADVERLELQGNNLTVIYETDFQRLTKLRMLQLTDNQIHTIERNSFQDLVSLERLRLNNNRLKAIPENFVTSSASLLRLDISNNVITTVGRRVFKGAQSLRSLQLDNNQITCLDEHAFKGLVELEILTLNNNNLTSLPHNIFGGLGRLRALRLSDNPFACDCHLSWLSRFLRSATRLAPYTR---CQSPSQLKGQNVADLHDQEFKCSG--LTEHAPMECGAENSCPHPCRCAD-----------------GIVDCREKSLTSVPVTLPDDTTELRLEQNFITELPPKSFSSFRRLRRIDLSNNNISRIAHDALSGLKQLTTLVLYGNKIKDLPSGVFKGLGSLQLLLLNANEISCIRKDAFRDLHSLSLLSLYDNNIQSLANGTFDAMKSIKTVHLAKNPFICDCNLRWLADYLHKNPIETSGARCESPKRMHRRRIESLREEKFKCSWDELRMKLSGEC 537          
BLAST of slit1 vs. Ensembl Zebrafish
Match: slit2 (slit homolog 2 (Drosophila) [Source:ZFIN;Acc:ZDB-GENE-010306-3])

HSP 1 Score: 681.789 bits (1758), Expect = 0.000e+0
Identity = 456/1378 (33.09%), Postives = 702/1378 (50.94%), Query Frame = 1
Query:  148 SEICPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQLQ------------------------CINDNIFNKLGMLKYLLLEGNKIFWISEA-ISKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCASEDKANLENMCPSDKKVCSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQIYNFQCN--KEFESKYETHDEECVPKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLPPIYSIHEAPNLMKLDLSFNQIETLEPDVFTRSS------------------------KLREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQFHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLC-DGNASVYHSVDSCHETNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVLSFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGN-YECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYC-NDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNSIYF-QKNSYIEISPPSRGFLMPRGNISLNFMTNSSSGLILYQGSEQTYLVVNLYQGHLRISYNLGDKVVGPEG-YSMGMVNDSRLHALHIEIVGENLTMYLNGRVHCTLKSKYLNSNRYMALDTGIFIGGAPHKILQSAIMKFHIETDSEFIGCMESLLINRKKIDFSKYLPKSINVHPGC 4113
            ++ CP+ C C   +V+C  +   +VP  +     RL+L  N +  I K+DF  L NL+VLQL  N+I+SIE GAF +L  L +LRLNRN +Q LP+ LF   TKL +LDL +NQ+Q                        CI D  F  L  L+ L L  N I  +S A  + +  L+  R+H N L C+C++AWL+ +L+Q+  +   G ++  C  P  L    I+ +   EF C        ++        C + C CS++ +V C GK L+ IP +LP  + E+ L+ N+I I+  G  + YK L++I L+ N + ++   +F GL  L S+ L  N +  + K  F GL SL  L L  N I CL  D+ +  + L+LL L +NKL ++ K TF     ++ L+L  NP  CDC + W+ DY++D    T  A C  PR L    +  ++   F+C+  +++ SK      +C   ++CP KC C    V+CS   L  +P+ I   T  L +++N    L       + P L K++LS N+I  +E   F  +S                         LR + L SNR+ C+NN +F  L  + +L L +N I+ +   +F    +L  L L  N F+C+C L W+ EW R    V GN   P CQSP  LK+ PI  +  + F C +GN       +SC         CS                    T V CS+  LK +P  IP+D TELYLD N    +      N  +L  + LS N I+ L+ +  + +  L TL+LS+N+L+CI   AF  L +L++L L  ND++ IP  AF DL +L+++ALG NP +CDC+++WL+ +    + + GI+ C  P  M  K +  +    F C+    D  I AKC+ C+  PCK++G CS      Y+C CPY F G++C++ I+AC   PC+NGG C   D   N + CVCP GF G +C IN+DDC  N C N +TC D  N+Y+C C   + G+ C+ K  +C +++N CQ+  +C +L    + C C+ G+ G+ CE + DDC+ N CK+ +QC+D  N Y C+C  GY G FCE     P VL                          S C +++C N   C+  D +      C+C PGY G  C+ L S+ F  + S+++I  PS   +  + NISL   T+  +G++LY+G  + ++ V LY+G LR+SY+ G     P   YS+  +ND   H + +    ++L++ ++G    ++ +   NS   +     +++GG P +   +++ +      S F GC+ +L IN +  D +++L +   V PGC
Sbjct:   18 AQSCPSQCSCSGTAVDCHGQSLRSVPRNIPRNVERLDLNANNLTKITKADFAGLKNLRVLQLMENKISSIERGAFQDLQELERLRLNRNNLQVLPELLFLGTTKLFRLDLSENQIQGIPRKAFRGSTEIKNLQLDYNQISCIEDGAFRALRDLEVLTLNNNNISRLSVASFNHMPKLRTFRLHSNNLLCDCNVAWLSDWLRQRPRL---GLYTQ-CMAPPSLRGHNIAEVQKKEFMCTGP-----QSHSSCSVLQCPELCTCSNN-VVDCRGKGLTEIPTNLPETITEIRLEQNSIKIIPAGAFAPYKRLRRIDLSNNQITELASDSFQGLRSLNSLVLYGNKITELPKGLFDGLFSLQLLLLNANKINCLRVDSFQDLQNLNLLSLYDNKLQTIAKGTFSSLRAIQTLHLAQNPFMCDCHLKWLADYLQDNPIETSGARCTSPRRLANKRIGQIKSKKFRCSGVEDYRSKL---GGDCFADLACPEKCRCEGTTVDCSGQKLTKIPDHIPQYTAELRLNNNEFTVLEATGIFKKLPQLRKINLSNNKITDIEEGTFEGASGVNELILTSNRLEGVHYSMLKGLGGLRTLMLRSNRISCVNNGSFTGLSSVRLLSLYDNLITSMSPGAFDTLHSLSTLNLLANPFNCNCHLAWLGEWLRKKRIVTGN---PRCQSPYFLKEIPIQDVAVQDFACEEGNDE-----NSCSPLARCPAECSC-----------------LDTVVRCSNKGLKVLPKGIPRDVTELYLDGNEFTQV-PLELSNYKHLTLIDLSNNQISTLSNHSFSNMSELLTLILSYNRLRCIPAKAFDGLKSLRLLSLHGNDIAVIPDGAFKDLSSLSHLALGANPLYCDCHMQWLSDWVKSGYKEPGIARCTGPGDMTDKLLLTTPSKKFTCTGPV-DVSILAKCNPCLSNPCKNDGTCSNHPVDFYRCTCPYGFKGQDCEEPIHACISNPCQNGGTCHLKDGEENTHWCVCPEGFEGDECEINIDDCEDNDCENNSTCVDGINNYTCLCSPEYTGELCEDKLDFCASELNLCQHDSKC-ILTAKGFMCECTPGYTGEHCEVDFDDCEDNKCKNGAQCIDAVNGYTCVCPEGYSGLFCEFSP--PMVLP-----------------------RTSPCDHYDCANGAQCVVKDTDP----VCQCLPGYEGVHCERLVSVNFINRESFLQI--PSN-LITEQANISLQIATDEDNGVLLYKGDNE-HIAVELYRGRLRVSYDSGS--YPPSAIYSVETINDGSFHVVELVAKDQSLSLSIDGGSPKSINTA--NSPSPVPSPAPLYLGGLPQQSGLASLRQGSGRNGSSFHGCIRNLYINDQLQDLTQFLLQE-GVVPGC 1316          

HSP 2 Score: 57.7658 bits (138), Expect = 1.414e-7
Identity = 40/121 (33.06%), Postives = 63/121 (52.07%), Query Frame = 1
Query: 2899 QPCRNG----GICKNIDNYGNYECVCPVGFRGPKCTINVDD-CVGNLCVNGATCNDLNN-SYSCKCQSGFRGKYCDIKFLYCNDINPCQ----NSGQCQLLPNNDYKCVCSKGWEGKQCER 3231
            QPC+      G C +     ++ C C  G+ GP C   V++ C GN C++G +C  +N+ SYSC+C  GF G  CD +      ++PCQ      G+C++       C C+ G+ G+ C+R
Sbjct: 1317 QPCQRSMCAHGQC-HATGQSSFSCECEAGWTGPLCDQQVNNPCDGNKCIHG-SCMAINSYSYSCRCLPGFAGVLCDEE----EQLSPCQYIACKYGRCRVSGLGKAYCECNSGYTGQSCDR 1431          

HSP 3 Score: 56.225 bits (134), Expect = 4.057e-7
Identity = 41/140 (29.29%), Postives = 65/140 (46.43%), Query Frame = 1
Query: 3010 CVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQC--ERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRR---DINQSEMT 3414
            C  ++C +G       +S+SC+C++G+ G  CD +     D N C + G C  + +  Y C C  G+ G  C  E     C+Y +CK+    V       C C  GY G+ C+  A +  +  T + +R     N SE+T
Sbjct: 1319 CQRSMCAHGQCHATGQSSFSCECEAGWTGPLCDQQVNNPCDGNKCIH-GSCMAINSYSYSCRCLPGFAGVLCDEEEQLSPCQYIACKYGRCRVSGLGKAYCECNSGYTGQSCDRAATESGMDPTQVRQRWRVGGNGSEIT 1457          

HSP 4 Score: 53.5286 bits (127), Expect = 2.919e-6
Identity = 41/150 (27.33%), Postives = 59/150 (39.33%), Query Frame = 1
Query: 3139 PCQNS----GQCQLLPNNDYKCVCSKGWEGKQCERNTDD-CKYNSCKHESQCVDLHN-DYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISES---VCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLC 3561
            PCQ S    GQC     + + C C  GW G  C++  ++ C  N C H S C+ +++  Y C C PG+ G  C+                            E E +S      C Y  C  +G+          K+YC+C  GY+G  C
Sbjct: 1318 PCQRSMCAHGQCHATGQSSFSCECEAGWTGPLCDQQVNNPCDGNKCIHGS-CMAINSYSYSCRCLPGFAGVLCD----------------------------EEEQLSPCQYIACKYGRCRVSGL---------GKAYCECNSGYTGQSC 1429          
BLAST of slit1 vs. Ensembl Zebrafish
Match: slit2 (slit homolog 2 (Drosophila) [Source:ZFIN;Acc:ZDB-GENE-010306-3])

HSP 1 Score: 680.248 bits (1754), Expect = 0.000e+0
Identity = 456/1378 (33.09%), Postives = 702/1378 (50.94%), Query Frame = 1
Query:  148 SEICPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQLQ------------------------CINDNIFNKLGMLKYLLLEGNKIFWISEA-ISKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCASEDKANLENMCPSDKKVCSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQIYNFQCN--KEFESKYETHDEECVPKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLPPIYSIHEAPNLMKLDLSFNQIETLEPDVFTRSS------------------------KLREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQFHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLC-DGNASVYHSVDSCHETNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVLSFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGN-YECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYC-NDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNSIYF-QKNSYIEISPPSRGFLMPRGNISLNFMTNSSSGLILYQGSEQTYLVVNLYQGHLRISYNLGDKVVGPEG-YSMGMVNDSRLHALHIEIVGENLTMYLNGRVHCTLKSKYLNSNRYMALDTGIFIGGAPHKILQSAIMKFHIETDSEFIGCMESLLINRKKIDFSKYLPKSINVHPGC 4113
            ++ CP+ C C   +V+C  +   +VP  +     RL+L  N +  I K+DF  L NL+VLQL  N+I+SIE GAF +L  L +LRLNRN +Q LP+ LF   TKL +LDL +NQ+Q                        CI D  F  L  L+ L L  N I  +S A  + +  L+  R+H N L C+C++AWL+ +L+Q+  +   G ++  C  P  L    I+ +   EF C        ++        C + C CS++ +V C GK L+ IP +LP  + E+ L+ N+I I+  G  + YK L++I L+ N + ++   +F GL  L S+ L  N +  + K  F GL SL  L L  N I CL  D+ +  + L+LL L +NKL ++ K TF     ++ L+L  NP  CDC + W+ DY++D    T  A C  PR L    +  ++   F+C+  +++ SK      +C   ++CP KC C    V+CS   L  +P+ I   T  L +++N    L       + P L K++LS N+I  +E   F  +S                         LR + L SNR+ C+NN +F  L  + +L L +N I+ +   +F    +L  L L  N F+C+C L W+ EW R    V GN   P CQSP  LK+ PI  +  + F C +GN       +SC         CS                    T V CS+  LK +P  IP+D TELYLD N    +      N  +L  + LS N I+ L+ +  + +  L TL+LS+N+L+CI   AF  L +L++L L  ND++ IP  AF DL +L+++ALG NP +CDC+++WL+ +    + + GI+ C  P  M  K +  +    F C+    D  I AKC+ C+  PCK++G CS      Y+C CPY F G++C++ I+AC   PC+NGG C   D   N + CVCP GF G +C IN+DDC  N C N +TC D  N+Y+C C   + G+ C+ K  +C +++N CQ+  +C +L    + C C+ G+ G+ CE + DDC+ N CK+ +QC+D  N Y C+C  GY G FCE     P VL                          S C +++C N   C+  D +      C+C PGY G  C+ L S+ F  + S+++I  PS   +  + NISL   T+  +G++LY+G  + ++ V LY+G LR+SY+ G     P   YS+  +ND   H + +    ++L++ ++G    ++ +   NS   +     +++GG P +   +++ +      S F GC+ +L IN +  D +++L +   V PGC
Sbjct:   18 AQSCPSQCSCSGTAVDCHGQSLRSVPRNIPRNVERLDLNANNLTKITKADFAGLKNLRVLQLMENKISSIERGAFQDLQELERLRLNRNNLQVLPELLFLGTTKLFRLDLSENQIQGIPRKAFRGSTEIKNLQLDYNQISCIEDGAFRALRDLEVLTLNNNNISRLSVASFNHMPKLRTFRLHSNNLLCDCNVAWLSDWLRQRPRL---GLYTQ-CMAPPSLRGHNIAEVQKKEFMCTGP-----QSHSSCSVLQCPELCTCSNN-VVDCRGKGLTEIPTNLPETITEIRLEQNSIKIIPAGAFAPYKRLRRIDLSNNQITELASDSFQGLRSLNSLVLYGNKITELPKGLFDGLFSLQLLLLNANKINCLRVDSFQDLQNLNLLSLYDNKLQTIAKGTFSSLRAIQTLHLAQNPFMCDCHLKWLADYLQDNPIETSGARCTSPRRLANKRIGQIKSKKFRCSGVEDYRSKL---GGDCFADLACPEKCRCEGTTVDCSGQKLTKIPDHIPQYTAELRLNNNEFTVLEATGIFKKLPQLRKINLSNNKITDIEEGTFEGASGVNELILTSNRLEGVHYSMLKGLGGLRTLMLRSNRISCVNNGSFTGLSSVRLLSLYDNLITSMSPGAFDTLHSLSTLNLLANPFNCNCHLAWLGEWLRKKRIVTGN---PRCQSPYFLKEIPIQDVAVQDFACEEGNDE-----NSCSPLARCPAECSC-----------------LDTVVRCSNKGLKVLPKGIPRDVTELYLDGNEFTQV-PLELSNYKHLTLIDLSNNQISTLSNHSFSNMSELLTLILSYNRLRCIPAKAFDGLKSLRLLSLHGNDIAVIPDGAFKDLSSLSHLALGANPLYCDCHMQWLSDWVKSGYKEPGIARCTGPGDMTDKLLLTTPSKKFTCTGPV-DVSILAKCNPCLSNPCKNDGTCSNHPVDFYRCTCPYGFKGQDCEEPIHACISNPCQNGGTCHLKDGEENTHWCVCPEGFEGDECEINIDDCEDNDCENNSTCVDGINNYTCLCSPEYTGELCEDKLDFCASELNLCQHDSKC-ILTAKGFMCECTPGYTGEHCEVDFDDCEDNKCKNGAQCIDAVNGYTCVCPEGYSGLFCEFSP--PMVLP-----------------------RTSPCDHYDCANGAQCVVKDTDP----VCQCLPGYEGVHCERLVSVNFINRESFLQI--PSN-LITEQANISLQIATDEDNGVLLYKGDNE-HIAVELYRGRLRVSYDSGS--YPPSAIYSVETINDGSFHVVELVAKDQSLSLSIDGGSPKSINTA--NSPSPVPSPAPLYLGGLPQQSGLASLRQGSGRNGSSFHGCIRNLYINDQLQDLTQFLLQE-GVVPGC 1316          

HSP 2 Score: 57.3806 bits (137), Expect = 1.798e-7
Identity = 40/121 (33.06%), Postives = 63/121 (52.07%), Query Frame = 1
Query: 2899 QPCRNG----GICKNIDNYGNYECVCPVGFRGPKCTINVDD-CVGNLCVNGATCNDLNN-SYSCKCQSGFRGKYCDIKFLYCNDINPCQ----NSGQCQLLPNNDYKCVCSKGWEGKQCER 3231
            QPC+      G C +     ++ C C  G+ GP C   V++ C GN C++G +C  +N+ SYSC+C  GF G  CD +      ++PCQ      G+C++       C C+ G+ G+ C+R
Sbjct: 1317 QPCQRSMCAHGQC-HATGQSSFSCECEAGWTGPLCDQQVNNPCDGNKCIHG-SCMAINSYSYSCRCLPGFAGVLCDEE----EQLSPCQYIACKYGRCRVSGLGKAYCECNSGYTGQSCDR 1431          

HSP 3 Score: 52.7582 bits (125), Expect = 4.283e-6
Identity = 41/150 (27.33%), Postives = 59/150 (39.33%), Query Frame = 1
Query: 3139 PCQNS----GQCQLLPNNDYKCVCSKGWEGKQCERNTDD-CKYNSCKHESQCVDLHN-DYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISES---VCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLC 3561
            PCQ S    GQC     + + C C  GW G  C++  ++ C  N C H S C+ +++  Y C C PG+ G  C+                            E E +S      C Y  C  +G+          K+YC+C  GY+G  C
Sbjct: 1318 PCQRSMCAHGQCHATGQSSFSCECEAGWTGPLCDQQVNNPCDGNKCIHGS-CMAINSYSYSCRCLPGFAGVLCD----------------------------EEEQLSPCQYIACKYGRCRVSGL---------GKAYCECNSGYTGQSC 1429          

HSP 4 Score: 51.9878 bits (123), Expect = 7.346e-6
Identity = 34/113 (30.09%), Postives = 53/113 (46.90%), Query Frame = 1
Query: 3010 CVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQC--ERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCE 3342
            C  ++C +G       +S+SC+C++G+ G  CD +     D N C + G C  + +  Y C C  G+ G  C  E     C+Y +CK+    V       C C  GY G+ C+
Sbjct: 1319 CQRSMCAHGQCHATGQSSFSCECEAGWTGPLCDQQVNNPCDGNKCIH-GSCMAINSYSYSCRCLPGFAGVLCDEEEQLSPCQYIACKYGRCRVSGLGKAYCECNSGYTGQSCD 1430          
BLAST of slit1 vs. Ensembl Zebrafish
Match: slit2 (slit homolog 2 (Drosophila) [Source:ZFIN;Acc:ZDB-GENE-010306-3])

HSP 1 Score: 675.626 bits (1742), Expect = 0.000e+0
Identity = 456/1387 (32.88%), Postives = 703/1387 (50.68%), Query Frame = 1
Query:  148 SEICPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQLQ------------------------CINDNIFNKLGMLKYLLLEGNKIFWISEA-ISKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCASEDKANLENMCPSDKKVCSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQIYNFQCN--KEFESKYETHDEECVPKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLPPIYSIHEAPNLMKLDLSFNQIETLEPDVFTRSS------------------------KLREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQFHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLC-DGNASVYHSVDSCHETNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVLSFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGN-YECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGF---------RGKYCDIKFLYC-NDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNSIYF-QKNSYIEISPPSRGFLMPRGNISLNFMTNSSSGLILYQGSEQTYLVVNLYQGHLRISYNLGDKVVGPEG-YSMGMVNDSRLHALHIEIVGENLTMYLNGRVHCTLKSKYLNSNRYMALDTGIFIGGAPHKILQSAIMKFHIETDSEFIGCMESLLINRKKIDFSKYLPKSINVHPGC 4113
            ++ CP+ C C   +V+C  +   +VP  +     RL+L  N +  I K+DF  L NL+VLQL  N+I+SIE GAF +L  L +LRLNRN +Q LP+ LF   TKL +LDL +NQ+Q                        CI D  F  L  L+ L L  N I  +S A  + +  L+  R+H N L C+C++AWL+ +L+Q+  +   G ++  C  P  L    I+ +   EF C        ++        C + C CS++ +V C GK L+ IP +LP  + E+ L+ N+I I+  G  + YK L++I L+ N + ++   +F GL  L S+ L  N +  + K  F GL SL  L L  N I CL  D+ +  + L+LL L +NKL ++ K TF     ++ L+L  NP  CDC + W+ DY++D    T  A C  PR L    +  ++   F+C+  +++ SK      +C   ++CP KC C    V+CS   L  +P+ I   T  L +++N    L       + P L K++LS N+I  +E   F  +S                         LR + L SNR+ C+NN +F  L  + +L L +N I+ +   +F    +L  L L  N F+C+C L W+ EW R    V GN   P CQSP  LK+ PI  +  + F C +GN       +SC         CS                    T V CS+  LK +P  IP+D TELYLD N    +      N  +L  + LS N I+ L+ +  + +  L TL+LS+N+L+CI   AF  L +L++L L  ND++ IP  AF DL +L+++ALG NP +CDC+++WL+ +    + + GI+ C  P  M  K +  +    F C+    D  I AKC+ C+  PCK++G CS      Y+C CPY F G++C++ I+AC   PC+NGG C   D   N + CVCP GF G +C IN+DDC  N C N +TC D  N+Y+C C   +         +G+ C+ K  +C +++N CQ+  +C +L    + C C+ G+ G+ CE + DDC+ N CK+ +QC+D  N Y C+C  GY G FCE     P VL                          S C +++C N   C+  D +      C+C PGY G  C+ L S+ F  + S+++I  PS   +  + NISL   T+  +G++LY+G  + ++ V LY+G LR+SY+ G     P   YS+  +ND   H + +    ++L++ ++G    ++ +   NS   +     +++GG P +   +++ +      S F GC+ +L IN +  D +++L +   V PGC
Sbjct:   18 AQSCPSQCSCSGTAVDCHGQSLRSVPRNIPRNVERLDLNANNLTKITKADFAGLKNLRVLQLMENKISSIERGAFQDLQELERLRLNRNNLQVLPELLFLGTTKLFRLDLSENQIQGIPRKAFRGSTEIKNLQLDYNQISCIEDGAFRALRDLEVLTLNNNNISRLSVASFNHMPKLRTFRLHSNNLLCDCNVAWLSDWLRQRPRL---GLYTQ-CMAPPSLRGHNIAEVQKKEFMCTGP-----QSHSSCSVLQCPELCTCSNN-VVDCRGKGLTEIPTNLPETITEIRLEQNSIKIIPAGAFAPYKRLRRIDLSNNQITELASDSFQGLRSLNSLVLYGNKITELPKGLFDGLFSLQLLLLNANKINCLRVDSFQDLQNLNLLSLYDNKLQTIAKGTFSSLRAIQTLHLAQNPFMCDCHLKWLADYLQDNPIETSGARCTSPRRLANKRIGQIKSKKFRCSGVEDYRSKL---GGDCFADLACPEKCRCEGTTVDCSGQKLTKIPDHIPQYTAELRLNNNEFTVLEATGIFKKLPQLRKINLSNNKITDIEEGTFEGASGVNELILTSNRLEGVHYSMLKGLGGLRTLMLRSNRISCVNNGSFTGLSSVRLLSLYDNLITSMSPGAFDTLHSLSTLNLLANPFNCNCHLAWLGEWLRKKRIVTGN---PRCQSPYFLKEIPIQDVAVQDFACEEGNDE-----NSCSPLARCPAECSC-----------------LDTVVRCSNKGLKVLPKGIPRDVTELYLDGNEFTQV-PLELSNYKHLTLIDLSNNQISTLSNHSFSNMSELLTLILSYNRLRCIPAKAFDGLKSLRLLSLHGNDIAVIPDGAFKDLSSLSHLALGANPLYCDCHMQWLSDWVKSGYKEPGIARCTGPGDMTDKLLLTTPSKKFTCTGPV-DVSILAKCNPCLSNPCKNDGTCSNHPVDFYRCTCPYGFKGQDCEEPIHACISNPCQNGGTCHLKDGEENTHWCVCPEGFEGDECEINIDDCEDNDCENNSTCVDGINNYTCLCSPEYTAANNNEVEKGELCEDKLDFCASELNLCQHDSKC-ILTAKGFMCECTPGYTGEHCEVDFDDCEDNKCKNGAQCIDAVNGYTCVCPEGYSGLFCEFSP--PMVLP-----------------------RTSPCDHYDCANGAQCVVKDTDP----VCQCLPGYEGVHCERLVSVNFINRESFLQI--PSN-LITEQANISLQIATDEDNGVLLYKGDNE-HIAVELYRGRLRVSYDSGS--YPPSAIYSVETINDGSFHVVELVAKDQSLSLSIDGGSPKSINTA--NSPSPVPSPAPLYLGGLPQQSGLASLRQGSGRNGSSFHGCIRNLYINDQLQDLTQFLLQE-GVVPGC 1325          

HSP 2 Score: 57.3806 bits (137), Expect = 1.641e-7
Identity = 40/121 (33.06%), Postives = 63/121 (52.07%), Query Frame = 1
Query: 2899 QPCRNG----GICKNIDNYGNYECVCPVGFRGPKCTINVDD-CVGNLCVNGATCNDLNN-SYSCKCQSGFRGKYCDIKFLYCNDINPCQ----NSGQCQLLPNNDYKCVCSKGWEGKQCER 3231
            QPC+      G C +     ++ C C  G+ GP C   V++ C GN C++G +C  +N+ SYSC+C  GF G  CD +      ++PCQ      G+C++       C C+ G+ G+ C+R
Sbjct: 1326 QPCQRSMCAHGQC-HATGQSSFSCECEAGWTGPLCDQQVNNPCDGNKCIHG-SCMAINSYSYSCRCLPGFAGVLCDEE----EQLSPCQYIACKYGRCRVSGLGKAYCECNSGYTGQSCDR 1440          

HSP 3 Score: 53.1434 bits (126), Expect = 4.110e-6
Identity = 41/150 (27.33%), Postives = 59/150 (39.33%), Query Frame = 1
Query: 3139 PCQNS----GQCQLLPNNDYKCVCSKGWEGKQCERNTDD-CKYNSCKHESQCVDLHN-DYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISES---VCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLC 3561
            PCQ S    GQC     + + C C  GW G  C++  ++ C  N C H S C+ +++  Y C C PG+ G  C+                            E E +S      C Y  C  +G+          K+YC+C  GY+G  C
Sbjct: 1327 PCQRSMCAHGQCHATGQSSFSCECEAGWTGPLCDQQVNNPCDGNKCIHGS-CMAINSYSYSCRCLPGFAGVLCD----------------------------EEEQLSPCQYIACKYGRCRVSGL---------GKAYCECNSGYTGQSC 1438          

HSP 4 Score: 51.9878 bits (123), Expect = 7.415e-6
Identity = 34/113 (30.09%), Postives = 53/113 (46.90%), Query Frame = 1
Query: 3010 CVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQC--ERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCE 3342
            C  ++C +G       +S+SC+C++G+ G  CD +     D N C + G C  + +  Y C C  G+ G  C  E     C+Y +CK+    V       C C  GY G+ C+
Sbjct: 1328 CQRSMCAHGQCHATGQSSFSCECEAGWTGPLCDQQVNNPCDGNKCIH-GSCMAINSYSYSCRCLPGFAGVLCDEEEQLSPCQYIACKYGRCRVSGLGKAYCECNSGYTGQSCD 1439          
BLAST of slit1 vs. Ensembl Zebrafish
Match: slit2 (slit homolog 2 (Drosophila) [Source:ZFIN;Acc:ZDB-GENE-010306-3])

HSP 1 Score: 674.47 bits (1739), Expect = 0.000e+0
Identity = 456/1387 (32.88%), Postives = 703/1387 (50.68%), Query Frame = 1
Query:  148 SEICPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQLQ------------------------CINDNIFNKLGMLKYLLLEGNKIFWISEA-ISKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCASEDKANLENMCPSDKKVCSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQIYNFQCN--KEFESKYETHDEECVPKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLPPIYSIHEAPNLMKLDLSFNQIETLEPDVFTRSS------------------------KLREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQFHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLC-DGNASVYHSVDSCHETNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVLSFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGN-YECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGF---------RGKYCDIKFLYC-NDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNSIYF-QKNSYIEISPPSRGFLMPRGNISLNFMTNSSSGLILYQGSEQTYLVVNLYQGHLRISYNLGDKVVGPEG-YSMGMVNDSRLHALHIEIVGENLTMYLNGRVHCTLKSKYLNSNRYMALDTGIFIGGAPHKILQSAIMKFHIETDSEFIGCMESLLINRKKIDFSKYLPKSINVHPGC 4113
            ++ CP+ C C   +V+C  +   +VP  +     RL+L  N +  I K+DF  L NL+VLQL  N+I+SIE GAF +L  L +LRLNRN +Q LP+ LF   TKL +LDL +NQ+Q                        CI D  F  L  L+ L L  N I  +S A  + +  L+  R+H N L C+C++AWL+ +L+Q+  +   G ++  C  P  L    I+ +   EF C        ++        C + C CS++ +V C GK L+ IP +LP  + E+ L+ N+I I+  G  + YK L++I L+ N + ++   +F GL  L S+ L  N +  + K  F GL SL  L L  N I CL  D+ +  + L+LL L +NKL ++ K TF     ++ L+L  NP  CDC + W+ DY++D    T  A C  PR L    +  ++   F+C+  +++ SK      +C   ++CP KC C    V+CS   L  +P+ I   T  L +++N    L       + P L K++LS N+I  +E   F  +S                         LR + L SNR+ C+NN +F  L  + +L L +N I+ +   +F    +L  L L  N F+C+C L W+ EW R    V GN   P CQSP  LK+ PI  +  + F C +GN       +SC         CS                    T V CS+  LK +P  IP+D TELYLD N    +      N  +L  + LS N I+ L+ +  + +  L TL+LS+N+L+CI   AF  L +L++L L  ND++ IP  AF DL +L+++ALG NP +CDC+++WL+ +    + + GI+ C  P  M  K +  +    F C+    D  I AKC+ C+  PCK++G CS      Y+C CPY F G++C++ I+AC   PC+NGG C   D   N + CVCP GF G +C IN+DDC  N C N +TC D  N+Y+C C   +         +G+ C+ K  +C +++N CQ+  +C +L    + C C+ G+ G+ CE + DDC+ N CK+ +QC+D  N Y C+C  GY G FCE     P VL                          S C +++C N   C+  D +      C+C PGY G  C+ L S+ F  + S+++I  PS   +  + NISL   T+  +G++LY+G  + ++ V LY+G LR+SY+ G     P   YS+  +ND   H + +    ++L++ ++G    ++ +   NS   +     +++GG P +   +++ +      S F GC+ +L IN +  D +++L +   V PGC
Sbjct:   18 AQSCPSQCSCSGTAVDCHGQSLRSVPRNIPRNVERLDLNANNLTKITKADFAGLKNLRVLQLMENKISSIERGAFQDLQELERLRLNRNNLQVLPELLFLGTTKLFRLDLSENQIQGIPRKAFRGSTEIKNLQLDYNQISCIEDGAFRALRDLEVLTLNNNNISRLSVASFNHMPKLRTFRLHSNNLLCDCNVAWLSDWLRQRPRL---GLYTQ-CMAPPSLRGHNIAEVQKKEFMCTGP-----QSHSSCSVLQCPELCTCSNN-VVDCRGKGLTEIPTNLPETITEIRLEQNSIKIIPAGAFAPYKRLRRIDLSNNQITELASDSFQGLRSLNSLVLYGNKITELPKGLFDGLFSLQLLLLNANKINCLRVDSFQDLQNLNLLSLYDNKLQTIAKGTFSSLRAIQTLHLAQNPFMCDCHLKWLADYLQDNPIETSGARCTSPRRLANKRIGQIKSKKFRCSGVEDYRSKL---GGDCFADLACPEKCRCEGTTVDCSGQKLTKIPDHIPQYTAELRLNNNEFTVLEATGIFKKLPQLRKINLSNNKITDIEEGTFEGASGVNELILTSNRLEGVHYSMLKGLGGLRTLMLRSNRISCVNNGSFTGLSSVRLLSLYDNLITSMSPGAFDTLHSLSTLNLLANPFNCNCHLAWLGEWLRKKRIVTGN---PRCQSPYFLKEIPIQDVAVQDFACEEGNDE-----NSCSPLARCPAECSC-----------------LDTVVRCSNKGLKVLPKGIPRDVTELYLDGNEFTQV-PLELSNYKHLTLIDLSNNQISTLSNHSFSNMSELLTLILSYNRLRCIPAKAFDGLKSLRLLSLHGNDIAVIPDGAFKDLSSLSHLALGANPLYCDCHMQWLSDWVKSGYKEPGIARCTGPGDMTDKLLLTTPSKKFTCTGPV-DVSILAKCNPCLSNPCKNDGTCSNHPVDFYRCTCPYGFKGQDCEEPIHACISNPCQNGGTCHLKDGEENTHWCVCPEGFEGDECEINIDDCEDNDCENNSTCVDGINNYTCLCSPEYTAANNNEVEKGELCEDKLDFCASELNLCQHDSKC-ILTAKGFMCECTPGYTGEHCEVDFDDCEDNKCKNGAQCIDAVNGYTCVCPEGYSGLFCEFSP--PMVLP-----------------------RTSPCDHYDCANGAQCVVKDTDP----VCQCLPGYEGVHCERLVSVNFINRESFLQI--PSN-LITEQANISLQIATDEDNGVLLYKGDNE-HIAVELYRGRLRVSYDSGS--YPPSAIYSVETINDGSFHVVELVAKDQSLSLSIDGGSPKSINTA--NSPSPVPSPAPLYLGGLPQQSGLASLRQGSGRNGSSFHGCIRNLYINDQLQDLTQFLLQE-GVVPGC 1325          

HSP 2 Score: 57.7658 bits (138), Expect = 1.452e-7
Identity = 40/121 (33.06%), Postives = 63/121 (52.07%), Query Frame = 1
Query: 2899 QPCRNG----GICKNIDNYGNYECVCPVGFRGPKCTINVDD-CVGNLCVNGATCNDLNN-SYSCKCQSGFRGKYCDIKFLYCNDINPCQ----NSGQCQLLPNNDYKCVCSKGWEGKQCER 3231
            QPC+      G C +     ++ C C  G+ GP C   V++ C GN C++G +C  +N+ SYSC+C  GF G  CD +      ++PCQ      G+C++       C C+ G+ G+ C+R
Sbjct: 1326 QPCQRSMCAHGQC-HATGQSSFSCECEAGWTGPLCDQQVNNPCDGNKCIHG-SCMAINSYSYSCRCLPGFAGVLCDEE----EQLSPCQYIACKYGRCRVSGLGKAYCECNSGYTGQSCDR 1440          

HSP 3 Score: 56.225 bits (134), Expect = 4.308e-7
Identity = 41/140 (29.29%), Postives = 65/140 (46.43%), Query Frame = 1
Query: 3010 CVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQC--ERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRR---DINQSEMT 3414
            C  ++C +G       +S+SC+C++G+ G  CD +     D N C + G C  + +  Y C C  G+ G  C  E     C+Y +CK+    V       C C  GY G+ C+  A +  +  T + +R     N SE+T
Sbjct: 1328 CQRSMCAHGQCHATGQSSFSCECEAGWTGPLCDQQVNNPCDGNKCIH-GSCMAINSYSYSCRCLPGFAGVLCDEEEQLSPCQYIACKYGRCRVSGLGKAYCECNSGYTGQSCDRAATESGMDPTQVRQRWRVGGNGSEIT 1466          

HSP 4 Score: 53.5286 bits (127), Expect = 3.099e-6
Identity = 41/150 (27.33%), Postives = 59/150 (39.33%), Query Frame = 1
Query: 3139 PCQNS----GQCQLLPNNDYKCVCSKGWEGKQCERNTDD-CKYNSCKHESQCVDLHN-DYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISES---VCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLC 3561
            PCQ S    GQC     + + C C  GW G  C++  ++ C  N C H S C+ +++  Y C C PG+ G  C+                            E E +S      C Y  C  +G+          K+YC+C  GY+G  C
Sbjct: 1327 PCQRSMCAHGQCHATGQSSFSCECEAGWTGPLCDQQVNNPCDGNKCIHGS-CMAINSYSYSCRCLPGFAGVLCD----------------------------EEEQLSPCQYIACKYGRCRVSGL---------GKAYCECNSGYTGQSC 1438          
BLAST of slit1 vs. Ensembl Zebrafish
Match: slit3 (slit homolog 3 (Drosophila) [Source:ZFIN;Acc:ZDB-GENE-010306-4])

HSP 1 Score: 671.774 bits (1732), Expect = 0.000e+0
Identity = 498/1585 (31.42%), Postives = 750/1585 (47.32%), Query Frame = 1
Query:  157 CPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQLQ------------------------CINDNIFNKLGMLKYLLLEGNKIFWIS-EAISKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCASEDKANLENMCPSDKKVCSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQIYNFQCN--KEFESKYETHDEECVPKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLPPIYSIHEAPNLMKLDLSFNQIETLEPDVFTRSS----------KLREIS--------------LNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQFHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCDGNAS--VYHSVDSCHETNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVLSFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNY-GNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYC-NDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNSIYF-QKNSYIEISPPSRGFLMPRGNISLNFMTNSSSGLILYQGSEQTYLVVNLYQGHLRISYNLGDKVVGPEGYSMGMVNDSRLHALHIEIVGENLTMYLNGRVHCTLKSKYLNSNRYMALDTGIFIGGAPHKILQSAIMKFHIETDSEFIGCMESLLINRKKIDFSKYLPK-----------SINVHPGCGKLISTVVASDLKIPSIMXXXXXXXXXXXXXXXNNVCKRPGICKNQGVCEVLPGSKKKFRCICKNGFVGRKCKKR--KSMCHG--------TFTES-----HIVDPDSQGTC--------KSIERFQYR-----ICTGTCSTSRMQSDVIKNFYHKPIDPSSSRCCQPTKYSTHEIQFKCENGRVYRRAANLPKNCKCV 4626
            CP+ C C    V+C+ + F  VP  +     RL+L  N I  I K DF  L NL+VL L NN+I+ IE GAF+ L  L ++RLNRN +Q LP+ LF   TKL +LDL +NQ+Q                        CI D  F  L  L+ L L  N I  I   + + +  L+ LR+H N L+C+CHL+WL+ +L+Q+  +    A    C  P  +  + +  +   EF C    +    + C     +C   C C+++ IV C  K L+ IP +LP  +VE+ L+ N I  +  G  S YK LK+I L++N + +I   AF GL  LTS+ L  N +  I K  F GLVSL  L L  N I CL  +T +  + L+LL L +NKL ++ K  F P   ++ L+L  NP  CDC + W+ DY+ D    T  A C  PR L    +  ++   F+C   +++ S+      EC   + CP +C C   VV+CSN  L  +P  +   T  L ++ N +  L    +  + PNL K++LS N++  +    F  +           KL+ +S              L SN++ C++N TF  L  + +L L +N IS I   +F    +L  + L  N + CDC L W+  W +    V GN   P CQ P  LK+ PI  +    F CDG        S   C E+ +    CS                    T V CS+  L+ +P  IPKDTTELYL+ N L S+ +    NL  L  L LS N I+ L     + +  L TL+LS+N+++C+   AF  L +L++L L  ND+ST+P  AFN L +L+++ALG NP +CDC ++WL+Q+    F + GI+ C  P  M  + +  +    F C +   D  + +KC  C+  PC++NG C      +Y C CP+ + G+NC+  INAC   PC NGG C       G Y CVCP G+ G +C +N DDC  N C N +TC D  N+Y+C C   ++G  C+     C    + CQ+  +C  L +  Y+C C  G+ G+QCE++ +DC  N C+H ++CVD  N Y C+C+ G+ G FCE     P VL                  L+N     S C   +C N   C+  +     +  C+C PGY G  CQ + +++F  K +Y+E+S      + P  +IS    T+  +G++LY+  +   L + LYQGH+R+ Y++  K      YS+  VND   H + + I  + L++ ++     +L    L     +  +T ++IGG P  I  S +     ++   F GC+  + +N +  D    L             S+     C +     V  D   P+           +     ++ C   G C  +G           + C+C +G+ G+ C ++   S C G          TES     H     S  TC        +++  + YR     ICT   STSR+Q    ++        SS  CC PTK    ++ FKC +G  +     +   C C 
Sbjct:   25 CPHKCSCSGSHVDCQGQAFKTVPRGIPRNAERLDLDRNNITRITKVDFSGLKNLRVLHLENNQISVIERGAFEGLKQLERIRLNRNRLQVLPELLFQSTTKLSRLDLSENQIQAVPRKAFRGITTVKNLQLDSNHISCIEDGAFRALRDLEILTLNNNNITLIPLSSFNHMPKLRTLRLHSNNLHCDCHLSWLSDWLRQRRGL----APFTQCMAPAHMRGLNVPDVQKREFVCTGPVETEPRS-CAPQVTICPAACTCNNN-IVDCRRKGLTEIPANLPEGIVEIRLEQNMIKNIPAGAFSTYKKLKRIDLSKNQISEIAEDAFSGLRSLTSLVLYGNKIAEIPKGLFDGLVSLQLLLLNANKINCLRVNTFKDLQNLNLLSLYDNKLQTISKGLFAPLRAIKTLHLAQNPFMCDCHLKWLADYLFDNPIETSGARCSHPRRLANKRISQVKGKKFRCTGAEDYRSRLSG---ECFQDLVCPERCRCEGTVVDCSNLKLTKIPPHLPEHTTDLRLNDNEISILEATGAFKKLPNLRKINLSNNKLRDIREGAFDGAGGVLELLLTGNKLQSVSGRMFKGLIGLKTLMLRSNQISCVDNATFTGLSSVRLLSLYDNRISTIAPGAFNTLHSLSTINLLSNPYVCDCHLAWLGLWLKKTRVVSGN---PRCQKPAFLKEIPIQDVAMPDFSCDGAEENVCLPSAPRCPESCT----CS-------------------ETVVRCSNRGLRSLPKTIPKDTTELYLEGNLLTSVPK-ELPNLKQLTLLDLSNNSISHLAPLTFSNMTQLATLILSYNQIRCVPVHAFDGLRSLRLLTLHGNDLSTVPEGAFNHLTSLSHLALGANPLYCDCELRWLSQWVKAGFKEPGIARCTGPPDMADRLLLTTPLNRFQC-KGPADLNLMSKCAPCLATPCQNNGTCVSDVTGSYHCTCPFGYKGRNCEIPINACISLPCTNGGTCHLTPGLDGQYSCVCPPGYEGQQCELNPDDCEDNDCENNSTCVDGINNYTCVCPPNYKGDLCEEVVDPCLPGFDLCQHDSKCVPL-SKGYRCECLPGYVGQQCEQDYNDCLENKCQHGAECVDAINGYTCVCKEGFSGLFCE--NHPPMVL------------------LQN----TSPCDQSDCQNGSECLVME----GEPVCRCLPGYFGTKCQKMVTLHFLGKEAYVELSGTR---IRPPMHISFQVATDKDNGILLYK-EDHDPLALELYQGHIRLIYDI-SKYPPTTVYSVESVNDGLFHTVEVLIQNQTLSLVVDKGTPKSLGK--LPRPPAVDHNTQLYIGGVPSLIGTSGLRPGPFQS---FNGCLHDVRLNGELQDLGAGLAGIEGVLFGCHSCSVCDQGACRQRGDEGVTCD--CPTGHSGPLCEQMSSQDPCESSRCVH-GECVAKG---------SSYSCLCVDGYSGQFCDRQDESSACKGLHCVYGECRLTESGEPACHCQPGYSGPTCETELTCEGEAVRDYLYRQMAHKICT---STSRVQRLECRH------SCSSGLCCAPTKSRRRKLSFKCPDGSSFSEEMEITVECGCT 1512          

HSP 2 Score: 289.656 bits (740), Expect = 7.392e-79
Identity = 212/655 (32.37%), Postives = 322/655 (49.16%), Query Frame = 1
Query:  154 ICPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQLQCINDNIFNKLGMLKYLLLEGNKIFWISEAI-SKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQC--ASEDKANLENMCPSDKKVCSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQ-GYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQIYNFQCNKEFESKYETHDEECVPKVS-CPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLPPIYSIHEAPNLMK---LDLSFNQIETLEPDVFTRSSKLREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQFHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCDGNASV 2094
            ICP  C C N  V+C+ +    +P  L E    + L  N I  I    F     LK + LS N+I+ I   AF  L +L  L L  N I  +P  LF  L  LQ L L  N++ C+  N F  L  L  L L  NK+  IS+ + + L+ +K L + +N   C+CHL WL  +L     I   GA    CS P RL++  IS +   +F+C  A + ++ L   C  D  VC ++C C    +V C   +L+ IP  LP +  +L L DN I+IL+  G   K   L+KI L+ N + DI  GAF G   +  + L  N L+++    F GL+ L  L L+ N I C+   T      + LL L +N+++++    F    +L  + L+ NP  CDC ++W+  ++K     +    C +P  L+  P++++ + +F C+   E+        C+P    CP  CTCS  VV CSN GL SLP++I   T  L +  N L  +P      E PNL +   LDLS N I  L P  F+  ++L  + L+ N+++C+    F  L+ L +L L  N +S +   +F +  +L +L L  N  +CDC+L+W+S+W +  +   G  R   C  P  + D  +       F C G A +
Sbjct:  271 ICPAACTCNNNIVDCRRKGLTEIPANLPEGIVEIRLEQNMIKNIPAGAFSTYKKLKRIDLSKNQISEIAEDAFSGLRSLTSLVLYGNKIAEIPKGLFDGLVSLQLLLLNANKINCLRVNTFKDLQNLNLLSLYDNKLQTISKGLFAPLRAIKTLHLAQNPFMCDCHLKWLADYLFDNP-IETSGAR---CSHPRRLANKRISQVKGKKFRCTGAEDYRSRLSGECFQD-LVCPERCRC-EGTVVDCSNLKLTKIPPHLPEHTTDLRLNDNEISILEATGAFKKLPNLRKINLSNNKLRDIREGAFDGAGGVLELLLTGNKLQSVSGRMFKGLIGLKTLMLRSNQISCVDNATFTGLSSVRLLSLYDNRISTIAPGAFNTLHSLSTINLLSNPYVCDCHLAWLGLWLKKTRVVSGNPRCQKPAFLKEIPIQDVAMPDFSCDGAEENV-------CLPSAPRCPESCTCSETVVRCSNRGLRSLPKTIPKDTTELYLEGNLLTSVP-----KELPNLKQLTLLDLSNNSISHLAPLTFSNMTQLATLILSYNQIRCVPVHAFDGLRSLRLLTLHGNDLSTVPEGAFNHLTSLSHLALGANPLYCDCELRWLSQWVKAGFKEPGIAR---CTGPPDMADRLLLTTPLNRFQCKGPADL 904          
BLAST of slit1 vs. Ensembl Xenopus
Match: zxdc (ZXD family zinc finger C [Source:Xenbase;Acc:XB-GENE-1033000])

HSP 1 Score: 714.531 bits (1843), Expect = 0.000e+0
Identity = 510/1615 (31.58%), Postives = 764/1615 (47.31%), Query Frame = 1
Query:  157 CPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQLQ------------------------CINDNIFNKLGMLKYLLLEGNKIFWIS-EAISKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCASEDKANLENMCPSDKKVCSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQIYNFQC--NKEFESKYETHDEECVPKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLPPIYSIHEAPNLMKLDLSFNQIETLEPDVFTRSSKLREISLNSNRLK------------------------CINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQFHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCDGNAS-----VYHSVDSCHETNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNE--TSFKNLLNLKTLV-LSYNGITELNKNVLTPLKSLETLVLSFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGIC-KNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYC-NDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNSIYF-QKNSYIEISPPSRGFLMPRGNISLNFMTNSSSGLILYQGSEQTYLVVNLYQGHLRISYNLGDKVVGPEG--YSMGMVNDSRLHALHIEIVGENLTMYLNGRVHCTLKSKYLNSNRYMALDTGIFIGGAPHKILQSAIMKFHIETDS---EFIGCMESLLINRKKIDFSKYLPKSINVHPGCGKLISTVVASDLKIPSIMXXXXXXXXXXXXXXXNNVCKRPGIC----KNQGVCEVLPG--------------------------SKKKFRCICKNGFVGRKCKKRK---SMC------HGTFTESHIVDP-------------DSQGTC-----KSIERFQ--YRICTGTCSTSRMQSDVIKNFYHKPIDPSSSRCCQPTKYSTHEIQFKCENGRVYRRAANLPKNCKC 4623
            CP+ C C   +V+C       VP  +     RL++  N I  I K+DF  L NL+VL L +N+I+ IE GAF +L  L ++RLN+N +Q LP+ LF    KL +LDL +NQ+Q                        CI D +F  L  L+ L L  N I  I   + + +  ++ LR+H N L C+CHL WL+ +L+Q+  I         C +P  L    ++ +   +F C           C ++   C   C CS++ +V C GK L+ IP +LP  ++E+ L+ N+I  +  G  + YK LK+I +++N + +I   AF GL  LTS+ L  N +  I K  F GLVSL  L L  N I CL  +T +  + L+LL L +NKL ++ K  F P  +++ L+L  NP  CDC + W+ DY++D    T  A C  PR L    +  ++   F+C  ++++ SK+     +C   + CP KC C   +V+CSN  +  +P  +   T  L ++ N +  L       + PNL K++LS N+I+ +    F  ++ ++E+ L  N+L+                        CINN+TF  L  + +L L +N I+ I   +F   ++L  + L  N F+C+C L W+ +W R    V GN   P CQ P  LK+ PI  +  + F CDGN         H  + C    SV                           V C +  L+ +P  IPKD TELYL+ NHL S+ +  +SFK+L    TL+ LS N I+ L+    + +  L TL+LS+N+L+CI   AF  L +L+VL L  ND+ST+P  +F+DL +L+++ALG NP +CDCN++WL+++    + + GI+ C+ PE M  + +  +    F C     D  I +KC+ C   PCK+NG C+      Y+C CP+ F G++CD  INAC   PC NGG C  N  N   + C CP+GF G KC IN DDC  N C N ATC D  N+Y C C   + G+ CD    +C  ++NPC +  +C +  +  Y C CS G+ G+ CE N DDC  + C+H +QC+D    Y C+C PG+ G FCEI    P VL                       +  S C  +EC N   CI       Q+  C+C  G++G  C+ L ++ F  K+SY+E+S  S   + P+ NISL   T+  +G++LY+G +   L + LYQGH+R+ Y   D +  P    YS+  +ND   H + + ++ ++L++ ++  +  +L    L     + L+T +++GG P     S +      TD     F GC+  + IN +  DF    P SI V PGC                                  N+CK  G C    K   +CE  PG                          +   + C C  G+ G+ C K+    S C      HG    S   DP             D +  C     + I R Q  Y  CT T    R +            + SS  CC P K    +  F+C +G  +         C C
Sbjct:   30 CPSKCTCSGPNVDCHGLGLKTVPKGIPRNAERLDMDKNNITRITKTDFAGLKNLRVLHLEDNQISIIERGAFQDLKQLERMRLNKNKLQVLPELLFQNNAKLTRLDLSENQIQGLPRKSFRGITDVKNLQLDNNQISCIEDGVFRALRELEILTLNNNNITRIPLTSFNHMPKIRTLRLHSNYLYCDCHLTWLSDWLRQRKTI----GQFTFCMSPVHLRGFNVADVQKKDFVCPGTQSE--PPSCNANAVSCPSACTCSNN-VVDCRGKGLTEIPANLPEGIIEIRLEQNSIKAIPAGVFTPYKKLKRIDISKNQISEIAADAFSGLKSLTSLVLYGNKITEIPKGLFDGLVSLQLLLLNANKINCLRVNTFQDLQNLNLLSLYDNKLQTISKGLFAPLQSIQTLHLAQNPFVCDCHLKWLADYLQDNPIETSGARCTSPRRLANKRISQIKSKKFRCTGSEDYRSKFSG---DCFMDLVCPEKCRCEGTIVDCSNQKISRIPSYLPEYTSDLRLNDNEITVLEATGIFKKLPNLRKINLSNNKIKDIREGAFDGAAGVQELMLTGNQLESVHGRMFRGLVGLKTLMLRSNLMSCINNDTFTGLSSVRLLSLYDNRITTITPGAFNTLVSLSTINLLSNPFNCNCHLAWLGKWLRKKRVVSGN---PRCQKPYFLKEIPIQDVAIQDFTCDGNEESSCLPASHCPEQCTCVESV---------------------------VRCGNQGLRTLPKNIPKDVTELYLEGNHLTSVPKEISSFKHL----TLIDLSNNSISVLSNYTFSNVTQLSTLILSYNRLRCIPVHAFNGLKSLRVLTLHGNDISTVPDGSFSDLTSLSHLALGTNPLYCDCNLRWLSEWVKAGYKEPGIARCSGPEDMADRLLLTTPTQKFQCKGPV-DMNIASKCNPCFSNPCKNNGTCNSDPVELYRCTCPFGFKGRDCDIPINACIQAPCENGGTCHTNEANKDGFSCSCPLGFEGEKCEINPDDCEDNDCENFATCVDGINNYICLCPPNYTGELCDEVIDHCVPELNPCLHDSKC-ISTDKGYMCECSPGFVGQNCETNEDDCANHKCRHGAQCIDDIGGYTCVCPPGFSGLFCEISP--PMVL-----------------------LQTSPCDNYECQNKAQCI----VVQQEPVCRCLAGFAGQRCEKLITVNFVGKDSYVELSGSS---VRPQANISLQVATDKDNGILLYKG-DNDPLALELYQGHVRLIY---DTLNSPPTTVYSVETINDGLFHTVELVMLNQSLSLVVDKGLPKSLGK--LQKQPSLNLNTPLYLGGIPPA---SGVPSMRQRTDHIPIGFNGCIHDVRINNELQDFRDLPPVSIGVFPGCKSC-------------------------------NICKH-GSCRSMEKTSVICECFPGWTGSMCDQEIDNTCINHKCIHGECVAANNAYMCKCAEGYTGQYCDKKNDSLSSCRNFKCNHGQCKLSERGDPYCECDPNFTGEHCDKENVCQGEVIREIVRKQHAYTSCTSTVRVPRTECSG---------NCSSGACCVPVKSRKRKYTFQCMDGTSFVEELERYIECGC 1516          

HSP 2 Score: 201.06 bits (510), Expect = 3.111e-51
Identity = 147/472 (31.14%), Postives = 232/472 (49.15%), Query Frame = 1
Query:  154 ICPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSD-FLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQLQCINDNIFNKLGMLKYLLLEGNKIFWISE-AISKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCASEDKANLENMC-PSDKKVCSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQIYNFQCNKEFESKYETHDEECVPKVSCPLK--CTCSSDVV 1554
            +CP  C+CE   V+C ++    +P  L E T+ L L  N I V+  +  F KL NL+ + LSNN+I  I  GAFD    + +L L  N ++ +   +F  L  L+ L LR N + CIN++ F  L  ++ L L  N+I  I+  A + L  L  + +  N  NCNCHLAWL  +L++K  +    + +  C  P+ L  IPI  ++  +F C      N E+ C P+    C ++C C  + +V+C  + L  +P+++P +V EL L+ N++T + +  +S +K L  I L+ N +  +    F  +TQL+++ L+ N L+ I  +AF GL SL  L L  NDI  +P                          +F    +L +L L  NPL CDC + W+ +++K       +A C  P ++    L       FQC    +    +   +C P  S P K   TC+SD V
Sbjct:  500 VCPEKCRCEGTIVDCSNQKISRIPSYLPEYTSDLRLNDNEITVLEATGIFKKLPNLRKINLSNNKIKDIREGAFDGAAGVQELMLTGNQLESVHGRMFRGLVGLKTLMLRSNLMSCINNDTFTGLSSVRLLSLYDNRITTITPGAFNTLVSLSTINLLSNPFNCNCHLAWLGKWLRKKRVV----SGNPRCQKPYFLKEIPIQDVAIQDFTC----DGNEESSCLPASH--CPEQCTCV-ESVVRCGNQGLRTLPKNIPKDVTELYLEGNHLTSVPK-EISSFKHLTLIDLSNNSISVLSNYTFSNVTQLSTLILSYNRLRCIPVHAFNGLKSLRVLTLHGNDISTVPDG------------------------SFSDLTSLSHLALGTNPLYCDCNLRWLSEWVKAGYKEPGIARCSGPEDMADRLLLTTPTQKFQCKGPVDMNIAS---KCNPCFSNPCKNNGTCNSDPV 932          
BLAST of slit1 vs. Ensembl Xenopus
Match: atad3a (ATPase family, AAA domain containing 3A [Source:Xenbase;Acc:XB-GENE-946129])

HSP 1 Score: 693.345 bits (1788), Expect = 0.000e+0
Identity = 470/1401 (33.55%), Postives = 711/1401 (50.75%), Query Frame = 1
Query:  157 CPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQLQ------------------------CINDNIFNKLGMLKYLLLEGNKIFWISEA-ISKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCASEDKANLENMCPSDKKVCSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQ----------------IYNFQCNKEFESKYETHDEECVPKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLPPIYSIHEAPNLMKLDLSFNQIETLEPDVFTRSSKLREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQ------------------------FHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCD-GNASVYHSVDSCHETNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVLSFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSE----------ETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGIC--KNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCN-DINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNSIYF-QKNSYIEISPPSRGFLMPRGNISLNFMTNSSSGLILYQGSEQTYLVVNLYQGHLRISYNLGDKVVGPEG--YSMGMVNDSRLHALHIEIVGENLTMYLNGRVHCTLKSKYLNSNRYMALDTGIFIGGAPHKILQSAIMKFHIETDSEFIGCMESLLINRKKIDFSKYLPKSINVHPGC 4113
            CP  C C   +V+C      +VP  +   T RL+L  N I  I K+DF  L +L++LQL  N+IT+IE GAF +L  L +LRLNRN +Q  P+ LF    KL +LDL +NQ+Q                        CI D  F  L  L+ L L  N I  +S A  + +  L+  R+H N L C+CHLAWL+ +L+Q+  +   G ++  C  P  L    I+ +   EF C  + ++ + + C      C   C CS++ IV C GK L+ IP +LP  + E+ L+ N+I ++  G  S YK L++I L+ N + +I   AF GL  L S+ L  N +  + K  F GLV L  L L  N I CL  D+ +    L+LL L +NKL ++ K TF P   ++ L+L  NP  CDC + W+ DY+      T  A C  PR L    +  ++                I++  C +++ SK      +C   ++CP KC C    V+CSN  L  +P+ I   T  L +++N    L       + P L K++LS N+I  +E   F  ++ + E+ L SNR++ + ++ FK L+ L+ L L  N ISC+  DSF    +++ L L  NQ                        F+C+C L W+ +W R    V GN   P CQ P  LK+ PI  +  + F CD GN       +SC   +     C+                    T V CS+  LK +P  IPK+ TELYLD N    L      N  +L  + LS N I+ L+ +  + +  L TL+LS+N+L+CI   AF  L +L++L L  NDVS IP  AFNDL +L+++A+G NP +CDCN++WL+ +    + + GI+ C+ P +M  K +  +    F C+E             D  I AKC+ C+  PCK++G C+      Y+C CPY F G++CD  I+AC   PCRN G C  K  D  G + C C  GF G  C +N+DDC  N C N +TC D  N+Y+C+C   + G+ C+ K  +C+ D+NPCQ+  +C +L    YKC C+ G+ G+ C+ + DDC+ N C++ ++CVD  N Y C+C  GY G FCE  +  P VL                          S C   EC N   CI     K  +  C+C PGY G+ C+ L S+ F  K+S+I+I P ++  +  + NI+L   T+  SG++LY+G ++  + V LY+G +R+SY+ G   + P    YS+  +ND   H + +  + + L++ ++G    ++ +  L     +++D+ ++IGG P K   + + +   +  + F+GC+ +L IN +  DF K L     V PGC
Sbjct:   37 CPAQCSCTGTTVDCHGLTLRSVPRNIPRNTERLDLNANNITRISKTDFAGLRHLRILQLMENKITTIERGAFQDLKELERLRLNRNNLQVFPELLFLGTPKLYRLDLSENQIQAIPRKAFRGAVDIKNLQLDYNQISCIEDGAFRALRDLEVLTLNNNNITRLSVASFNHMPKLRTFRLHSNNLYCDCHLAWLSDWLRQRPRV---GLYTQ-CMGPIHLRGHNIAEVQKREFVCTGQ-QSFMVHSCSVLH--CPAACTCSNN-IVDCRGKGLTEIPTNLPETITEMRLEQNSIKVIPPGAFSPYKKLRRIDLSNNQISEIAADAFQGLRSLNSLVLYGNKITELPKGLFEGLVLLQLLLLNANKINCLRVDSFQDLHNLNLLSLYDNKLQTIAKGTFAPLRAIQTLHLAQNPFICDCHLKWLADYLHTNPIETSGARCTSPRRLANKRIGQIKSKKFRCSAQEQYFIPGIHHLGCTEDYRSKLSG---DCFADLACPEKCRCEGTTVDCSNQKLTKIPDHIPQYTAELRLNNNEFTVLEATGIFKKLPQLRKINLSNNKITDIEEGAFEGANGVNELLLTSNRMENVRHKMFKGLEGLKTLMLRSNRISCVNNDSFTGLSSVRLLSLYDNQITTVAPGAFDTLHSLSTLNLLANPFNCNCHLAWLGDWLRKKRIVTGN---PRCQKPYFLKEIPIQDVAIQDFTCDDGNED-----NSCSPLSRCPAECTC-----------------LDTVVRCSNKGLKSLPKGIPKEVTELYLDGNMF-PLVPKELSNYKHLTLIDLSNNRISTLSNHSFSNMTQLLTLILSYNRLRCIPLRAFDGLKSLRLLSLHGNDVSAIPEGAFNDLSSLSHLAIGANPLYCDCNLQWLSDWVKSEYKEPGIARCSGPGEMADKLLLTTPSKKFTCNEIPGPCGFPIAGPVDVSILAKCNPCLSNPCKNDGTCNNDPVEFYRCTCPYGFKGQDCDVPIHACISNPCRNSGTCHLKEGDKDG-FWCACAEGFEGENCEVNIDDCEDNDCENNSTCVDGINNYTCRCPPEYTGELCEEKLDFCSQDVNPCQHDSKC-ILTLKGYKCDCTPGYIGEHCDIDYDDCQENKCRNGARCVDAVNGYTCVCPEGYSGLFCE--SAPPMVLP-----------------------RTSPCDNFECQNGAQCIV----KGNEPQCQCLPGYHGNKCEKLVSVNFVNKDSFIQI-PSAK--IRTQANITLQVATDEDSGILLYKG-DKDQVAVELYRGRVRVSYDTG---INPASAIYSVETINDGNFHVVELVNLDQTLSLVVDGGTPKSITN--LPKQSLISIDSTLYIGGMPMKNNIAGLRQSSSQNGTNFLGCIRNLYINNELQDFRK-LNLQAGVLPGC 1359          

HSP 2 Score: 58.5362 bits (140), Expect = 1.103e-7
Identity = 43/115 (37.39%), Postives = 58/115 (50.43%), Query Frame = 1
Query: 2917 GICKNIDNYGNYECVCPVGFRGPKCTINVDD-CVGNLCVNGATCNDLNN-SYSCKCQSGFRGKYCDIKFLYCNDINPCQN----SGQCQLLPNNDYKCVCSKGWEGKQCERNTDD 3243
            G C      G + C C  G+ G  C I  +D C+GN CV+G TC  +N+ SYSCKC  GF G  CD      +  NPCQ+     G+C+L       C C+ G+ G  C++   D
Sbjct: 1370 GTCLATSQAG-FTCECEGGWTGQLCDIQSNDPCLGNKCVHG-TCLPINSFSYSCKCLQGFVGVLCDEPE---DQPNPCQSIKCKHGKCRLSGLGKAYCECNSGYTGDSCDKAFTD 1479          
BLAST of slit1 vs. Ensembl Xenopus
Match: atad3a (ATPase family, AAA domain containing 3A [Source:Xenbase;Acc:XB-GENE-946129])

HSP 1 Score: 690.649 bits (1781), Expect = 0.000e+0
Identity = 468/1391 (33.64%), Postives = 708/1391 (50.90%), Query Frame = 1
Query:  157 CPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQLQ------------------------CINDNIFNKLGMLKYLLLEGNKIFWISEA-ISKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCASEDKANLENMCPSDKKVCSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQIYNFQCNKE-------FESKYETHDEECVPKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLPPIYSIHEAPNLMKLDLSFNQIETLEPDVFTRSSKLREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQ------------------------FHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCD-GNASVYHSVDSCHETNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVLSFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGIC--KNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGF---------RGKYCDIKFLYCN-DINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNSIYF-QKNSYIEISPPSRGFLMPRGNISLNFMTNSSSGLILYQGSEQTYLVVNLYQGHLRISYNLGDKVVGPEG--YSMGMVNDSRLHALHIEIVGENLTMYLNGRVHCTLKSKYLNSNRYMALDTGIFIGGAPHKILQSAIMKFHIETDSEFIGCMESLLINRKKIDFSKYLPKSINVHPGC 4113
            CP  C C   +V+C      +VP  +   T RL+L  N I  I K+DF  L +L++LQL  N+IT+IE GAF +L  L +LRLNRN +Q  P+ LF    KL +LDL +NQ+Q                        CI D  F  L  L+ L L  N I  +S A  + +  L+  R+H N L C+CHLAWL+ +L+Q+  +   G ++  C  P  L    I+ +   EF C  + ++ + + C      C   C CS++ IV C GK L+ IP +LP  + E+ L+ N+I ++  G  S YK L++I L+ N + +I   AF GL  L S+ L  N +  + K  F GLV L  L L  N I CL  D+ +    L+LL L +NKL ++ K TF P   ++ L+L  NP  CDC + W+ DY+      T  A C  PR L    +  ++   F+C+ +        E        +C   ++CP KC C    V+CSN  L  +P+ I   T  L +++N    L       + P L K++LS N+I  +E   F  ++ + E+ L SNR++ + ++ FK L+ L+ L L  N ISC+  DSF    +++ L L  NQ                        F+C+C L W+ +W R    V GN   P CQ P  LK+ PI  +  + F CD GN       +SC   +     C+                    T V CS+  LK +P  IPK+ TELYLD N    L      N  +L  + LS N I+ L+ +  + +  L TL+LS+N+L+CI   AF  L +L++L L  NDVS IP  AFNDL +L+++A+G NP +CDCN++WL+ +    + + GI+ C+ P +M  K +  +    F C+    D  I AKC+ C+  PCK++G C+      Y+C CPY F G++CD  I+AC   PCRN G C  K  D  G + C C  GF G  C +N+DDC  N C N +TC D  N+Y+C+C   +         +G+ C+ K  +C+ D+NPCQ+  +C +L    YKC C+ G+ G+ C+ + DDC+ N C++ ++CVD  N Y C+C  GY G FCE  +  P VL                          S C   EC N   CI     K  +  C+C PGY G+ C+ L S+ F  K+S+I+I P ++  +  + NI+L   T+  SG++LY+G ++  + V LY+G +R+SY+ G   + P    YS+  +ND   H + +  + + L++ ++G    ++ +  L     +++D+ ++IGG P K   + + +   +  + F+GC+ +L IN +  DF K L     V PGC
Sbjct:   28 CPAQCSCTGTTVDCHGLTLRSVPRNIPRNTERLDLNANNITRISKTDFAGLRHLRILQLMENKITTIERGAFQDLKELERLRLNRNNLQVFPELLFLGTPKLYRLDLSENQIQAIPRKAFRGAVDIKNLQLDYNQISCIEDGAFRALRDLEVLTLNNNNITRLSVASFNHMPKLRTFRLHSNNLYCDCHLAWLSDWLRQRPRV---GLYTQ-CMGPIHLRGHNIAEVQKREFVCTGQ-QSFMVHSCSVLH--CPAACTCSNN-IVDCRGKGLTEIPTNLPETITEMRLEQNSIKVIPPGAFSPYKKLRRIDLSNNQISEIAADAFQGLRSLNSLVLYGNKITELPKGLFEGLVLLQLLLLNANKINCLRVDSFQDLHNLNLLSLYDNKLQTIAKGTFAPLRAIQTLHLAQNPFICDCHLKWLADYLHTNPIETSGARCTSPRRLANKRIGQIKSKKFRCSAQEQYFIPGTEDYRSKLSGDCFADLACPEKCRCEGTTVDCSNQKLTKIPDHIPQYTAELRLNNNEFTVLEATGIFKKLPQLRKINLSNNKITDIEEGAFEGANGVNELLLTSNRMENVRHKMFKGLEGLKTLMLRSNRISCVNNDSFTGLSSVRLLSLYDNQITTVAPGAFDTLHSLSTLNLLANPFNCNCHLAWLGDWLRKKRIVTGN---PRCQKPYFLKEIPIQDVAIQDFTCDDGNED-----NSCSPLSRCPAECTC-----------------LDTVVRCSNKGLKSLPKGIPKEVTELYLDGNMF-PLVPKELSNYKHLTLIDLSNNRISTLSNHSFSNMTQLLTLILSYNRLRCIPLRAFDGLKSLRLLSLHGNDVSAIPEGAFNDLSSLSHLAIGANPLYCDCNLQWLSDWVKSEYKEPGIARCSGPGEMADKLLLTTPSKKFTCNGPV-DVSILAKCNPCLSNPCKNDGTCNNDPVEFYRCTCPYGFKGQDCDVPIHACISNPCRNSGTCHLKEGDKDG-FWCACAEGFEGENCEVNIDDCEDNDCENNSTCVDGINNYTCRCPPEYTAAKLNEVEKGELCEEKLDFCSQDVNPCQHDSKC-ILTLKGYKCDCTPGYIGEHCDIDYDDCQENKCRNGARCVDAVNGYTCVCPEGYSGLFCE--SAPPMVLP-----------------------RTSPCDNFECQNGAQCIV----KGNEPQCQCLPGYHGNKCEKLVSVNFVNKDSFIQI-PSAK--IRTQANITLQVATDEDSGILLYKG-DKDQVAVELYRGRVRVSYDTG---INPASAIYSVETINDGNFHVVELVNLDQTLSLVVDGGTPKSITN--LPKQSLISIDSTLYIGGMPMKNNIAGLRQSSSQNGTNFLGCIRNLYINNELQDFRK-LNLQAGVLPGC 1342          

HSP 2 Score: 57.7658 bits (138), Expect = 2.284e-7
Identity = 42/111 (37.84%), Postives = 57/111 (51.35%), Query Frame = 1
Query: 2917 GICKNIDNYGNYECVCPVGFRGPKCTINVDD-CVGNLCVNGATCNDLNN-SYSCKCQSGFRGKYCDIKFLYCNDINPCQN----SGQCQLLPNNDYKCVCSKGWEGKQCER 3231
            G C      G + C C  G+ G  C I  +D C+GN CV+G TC  +N+ SYSCKC  GF G  CD      +  NPCQ+     G+C+L       C C+ G+ G  C++
Sbjct: 1353 GTCLATSQAG-FTCECEGGWTGQLCDIQSNDPCLGNKCVHG-TCLPINSFSYSCKCLQGFVGVLCDEPE---DQPNPCQSIKCKHGKCRLSGLGKAYCECNSGYTGDSCDK 1458          
BLAST of slit1 vs. Ensembl Xenopus
Match: atad3a (ATPase family, AAA domain containing 3A [Source:Xenbase;Acc:XB-GENE-946129])

HSP 1 Score: 690.263 bits (1780), Expect = 0.000e+0
Identity = 470/1396 (33.67%), Postives = 712/1396 (51.00%), Query Frame = 1
Query:  157 CPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQLQ------------------------CINDNIFNKLGMLKYLLLEGNKIFWISEA-ISKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCASEDKANLENMCPSDKKVCSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQIYNFQCN--KEFESKYETHDEECVPKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLPPIYSIHEAPNLMKLDLSFNQIETLEPDVFTRSSKLREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQ------------------------FHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCD-GNASVYHSVDSCHETNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVLSFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSE----------ETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGIC--KNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGF---------RGKYCDIKFLYCN-DINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNSIYF-QKNSYIEISPPSRGFLMPRGNISLNFMTNSSSGLILYQGSEQTYLVVNLYQGHLRISYNLGDKVVGPEG--YSMGMVNDSRLHALHIEIVGENLTMYLNGRVHCTLKSKYLNSNRYMALDTGIFIGGAPHKILQSAIMKFHIETDSEFIGCMESLLINRKKIDFSKYLPKSINVHPGC 4113
            CP  C C   +V+C      +VP  +   T RL+L  N I  I K+DF  L +L++LQL  N+IT+IE GAF +L  L +LRLNRN +Q  P+ LF    KL +LDL +NQ+Q                        CI D  F  L  L+ L L  N I  +S A  + +  L+  R+H N L C+CHLAWL+ +L+Q+  +   G ++  C  P  L    I+ +   EF C  + ++ + + C      C   C CS++ IV C GK L+ IP +LP  + E+ L+ N+I ++  G  S YK L++I L+ N + +I   AF GL  L S+ L  N +  + K  F GLV L  L L  N I CL  D+ +    L+LL L +NKL ++ K TF P   ++ L+L  NP  CDC + W+ DY+      T  A C  PR L    +  ++   F+C+  +++ SK      +C   ++CP KC C    V+CSN  L  +P+ I   T  L +++N    L       + P L K++LS N+I  +E   F  ++ + E+ L SNR++ + ++ FK L+ L+ L L  N ISC+  DSF    +++ L L  NQ                        F+C+C L W+ +W R    V GN   P CQ P  LK+ PI  +  + F CD GN       +SC   +     C+                    T V CS+  LK +P  IPK+ TELYLD N    L      N  +L  + LS N I+ L+ +  + +  L TL+LS+N+L+CI   AF  L +L++L L  NDVS IP  AFNDL +L+++A+G NP +CDCN++WL+ +    + + GI+ C+ P +M  K +  +    F C+E             D  I AKC+ C+  PCK++G C+      Y+C CPY F G++CD  I+AC   PCRN G C  K  D  G + C C  GF G  C +N+DDC  N C N +TC D  N+Y+C+C   +         +G+ C+ K  +C+ D+NPCQ+  +C +L    YKC C+ G+ G+ C+ + DDC+ N C++ ++CVD  N Y C+C  GY G FCE  +  P VL                          S C   EC N   CI     K  +  C+C PGY G+ C+ L S+ F  K+S+I+I P ++  +  + NI+L   T+  SG++LY+G ++  + V LY+G +R+SY+ G   + P    YS+  +ND   H + +  + + L++ ++G    ++ +  L     +++D+ ++IGG P K   + + +   +  + F+GC+ +L IN +  DF K L     V PGC
Sbjct:   37 CPAQCSCTGTTVDCHGLTLRSVPRNIPRNTERLDLNANNITRISKTDFAGLRHLRILQLMENKITTIERGAFQDLKELERLRLNRNNLQVFPELLFLGTPKLYRLDLSENQIQAIPRKAFRGAVDIKNLQLDYNQISCIEDGAFRALRDLEVLTLNNNNITRLSVASFNHMPKLRTFRLHSNNLYCDCHLAWLSDWLRQRPRV---GLYTQ-CMGPIHLRGHNIAEVQKREFVCTGQ-QSFMVHSCSVLH--CPAACTCSNN-IVDCRGKGLTEIPTNLPETITEMRLEQNSIKVIPPGAFSPYKKLRRIDLSNNQISEIAADAFQGLRSLNSLVLYGNKITELPKGLFEGLVLLQLLLLNANKINCLRVDSFQDLHNLNLLSLYDNKLQTIAKGTFAPLRAIQTLHLAQNPFICDCHLKWLADYLHTNPIETSGARCTSPRRLANKRIGQIKSKKFRCSGTEDYRSKLSG---DCFADLACPEKCRCEGTTVDCSNQKLTKIPDHIPQYTAELRLNNNEFTVLEATGIFKKLPQLRKINLSNNKITDIEEGAFEGANGVNELLLTSNRMENVRHKMFKGLEGLKTLMLRSNRISCVNNDSFTGLSSVRLLSLYDNQITTVAPGAFDTLHSLSTLNLLANPFNCNCHLAWLGDWLRKKRIVTGN---PRCQKPYFLKEIPIQDVAIQDFTCDDGNED-----NSCSPLSRCPAECTC-----------------LDTVVRCSNKGLKSLPKGIPKEVTELYLDGNMF-PLVPKELSNYKHLTLIDLSNNRISTLSNHSFSNMTQLLTLILSYNRLRCIPLRAFDGLKSLRLLSLHGNDVSAIPEGAFNDLSSLSHLAIGANPLYCDCNLQWLSDWVKSEYKEPGIARCSGPGEMADKLLLTTPSKKFTCNEIPGPCGFPIAGPVDVSILAKCNPCLSNPCKNDGTCNNDPVEFYRCTCPYGFKGQDCDVPIHACISNPCRNSGTCHLKEGDKDG-FWCACAEGFEGENCEVNIDDCEDNDCENNSTCVDGINNYTCRCPPEYTAAKLNEVEKGELCEEKLDFCSQDVNPCQHDSKC-ILTLKGYKCDCTPGYIGEHCDIDYDDCQENKCRNGARCVDAVNGYTCVCPEGYSGLFCE--SAPPMVLP-----------------------RTSPCDNFECQNGAQCIV----KGNEPQCQCLPGYHGNKCEKLVSVNFVNKDSFIQI-PSAK--IRTQANITLQVATDEDSGILLYKG-DKDQVAVELYRGRVRVSYDTG---INPASAIYSVETINDGNFHVVELVNLDQTLSLVVDGGTPKSITN--LPKQSLISIDSTLYIGGMPMKNNIAGLRQSSSQNGTNFLGCIRNLYINNELQDFRK-LNLQAGVLPGC 1354          

HSP 2 Score: 57.3806 bits (137), Expect = 2.406e-7
Identity = 42/111 (37.84%), Postives = 57/111 (51.35%), Query Frame = 1
Query: 2917 GICKNIDNYGNYECVCPVGFRGPKCTINVDD-CVGNLCVNGATCNDLNN-SYSCKCQSGFRGKYCDIKFLYCNDINPCQN----SGQCQLLPNNDYKCVCSKGWEGKQCER 3231
            G C      G + C C  G+ G  C I  +D C+GN CV+G TC  +N+ SYSCKC  GF G  CD      +  NPCQ+     G+C+L       C C+ G+ G  C++
Sbjct: 1365 GTCLATSQAG-FTCECEGGWTGQLCDIQSNDPCLGNKCVHG-TCLPINSFSYSCKCLQGFVGVLCDEPE---DQPNPCQSIKCKHGKCRLSGLGKAYCECNSGYTGDSCDK 1470          
BLAST of slit1 vs. Ensembl Xenopus
Match: atad3a (ATPase family, AAA domain containing 3A [Source:Xenbase;Acc:XB-GENE-946129])

HSP 1 Score: 689.108 bits (1777), Expect = 0.000e+0
Identity = 469/1400 (33.50%), Postives = 711/1400 (50.79%), Query Frame = 1
Query:  157 CPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQLQ------------------------CINDNIFNKLGMLKYLLLEGNKIFWISEA-ISKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCASEDKANLENMCPSDKKVCSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQ----------------IYNFQCNKEFESKYETHDEECVPKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLPPIYSIHEAPNLMKLDLSFNQIETLEPDVFTRSSKLREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQ------------------------FHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCD-GNASVYHSVDSCHETNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVLSFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGIC--KNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGF---------RGKYCDIKFLYCN-DINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNSIYF-QKNSYIEISPPSRGFLMPRGNISLNFMTNSSSGLILYQGSEQTYLVVNLYQGHLRISYNLGDKVVGPEG--YSMGMVNDSRLHALHIEIVGENLTMYLNGRVHCTLKSKYLNSNRYMALDTGIFIGGAPHKILQSAIMKFHIETDSEFIGCMESLLINRKKIDFSKYLPKSINVHPGC 4113
            CP  C C   +V+C      +VP  +   T RL+L  N I  I K+DF  L +L++LQL  N+IT+IE GAF +L  L +LRLNRN +Q  P+ LF    KL +LDL +NQ+Q                        CI D  F  L  L+ L L  N I  +S A  + +  L+  R+H N L C+CHLAWL+ +L+Q+  +   G ++  C  P  L    I+ +   EF C  + ++ + + C      C   C CS++ IV C GK L+ IP +LP  + E+ L+ N+I ++  G  S YK L++I L+ N + +I   AF GL  L S+ L  N +  + K  F GLV L  L L  N I CL  D+ +    L+LL L +NKL ++ K TF P   ++ L+L  NP  CDC + W+ DY+      T  A C  PR L    +  ++                I++  C +++ SK      +C   ++CP KC C    V+CSN  L  +P+ I   T  L +++N    L       + P L K++LS N+I  +E   F  ++ + E+ L SNR++ + ++ FK L+ L+ L L  N ISC+  DSF    +++ L L  NQ                        F+C+C L W+ +W R    V GN   P CQ P  LK+ PI  +  + F CD GN       +SC   +     C+                    T V CS+  LK +P  IPK+ TELYLD N    L      N  +L  + LS N I+ L+ +  + +  L TL+LS+N+L+CI   AF  L +L++L L  NDVS IP  AFNDL +L+++A+G NP +CDCN++WL+ +    + + GI+ C+ P +M  K +  +    F C+    D  I AKC+ C+  PCK++G C+      Y+C CPY F G++CD  I+AC   PCRN G C  K  D  G + C C  GF G  C +N+DDC  N C N +TC D  N+Y+C+C   +         +G+ C+ K  +C+ D+NPCQ+  +C +L    YKC C+ G+ G+ C+ + DDC+ N C++ ++CVD  N Y C+C  GY G FCE  +  P VL                          S C   EC N   CI     K  +  C+C PGY G+ C+ L S+ F  K+S+I+I P ++  +  + NI+L   T+  SG++LY+G ++  + V LY+G +R+SY+ G   + P    YS+  +ND   H + +  + + L++ ++G    ++ +  L     +++D+ ++IGG P K   + + +   +  + F+GC+ +L IN +  DF K L     V PGC
Sbjct:   37 CPAQCSCTGTTVDCHGLTLRSVPRNIPRNTERLDLNANNITRISKTDFAGLRHLRILQLMENKITTIERGAFQDLKELERLRLNRNNLQVFPELLFLGTPKLYRLDLSENQIQAIPRKAFRGAVDIKNLQLDYNQISCIEDGAFRALRDLEVLTLNNNNITRLSVASFNHMPKLRTFRLHSNNLYCDCHLAWLSDWLRQRPRV---GLYTQ-CMGPIHLRGHNIAEVQKREFVCTGQ-QSFMVHSCSVLH--CPAACTCSNN-IVDCRGKGLTEIPTNLPETITEMRLEQNSIKVIPPGAFSPYKKLRRIDLSNNQISEIAADAFQGLRSLNSLVLYGNKITELPKGLFEGLVLLQLLLLNANKINCLRVDSFQDLHNLNLLSLYDNKLQTIAKGTFAPLRAIQTLHLAQNPFICDCHLKWLADYLHTNPIETSGARCTSPRRLANKRIGQIKSKKFRCSAQEQYFIPGIHHLGCTEDYRSKLSG---DCFADLACPEKCRCEGTTVDCSNQKLTKIPDHIPQYTAELRLNNNEFTVLEATGIFKKLPQLRKINLSNNKITDIEEGAFEGANGVNELLLTSNRMENVRHKMFKGLEGLKTLMLRSNRISCVNNDSFTGLSSVRLLSLYDNQITTVAPGAFDTLHSLSTLNLLANPFNCNCHLAWLGDWLRKKRIVTGN---PRCQKPYFLKEIPIQDVAIQDFTCDDGNED-----NSCSPLSRCPAECTC-----------------LDTVVRCSNKGLKSLPKGIPKEVTELYLDGNMF-PLVPKELSNYKHLTLIDLSNNRISTLSNHSFSNMTQLLTLILSYNRLRCIPLRAFDGLKSLRLLSLHGNDVSAIPEGAFNDLSSLSHLAIGANPLYCDCNLQWLSDWVKSEYKEPGIARCSGPGEMADKLLLTTPSKKFTCNGPV-DVSILAKCNPCLSNPCKNDGTCNNDPVEFYRCTCPYGFKGQDCDVPIHACISNPCRNSGTCHLKEGDKDG-FWCACAEGFEGENCEVNIDDCEDNDCENNSTCVDGINNYTCRCPPEYTAAKLNEVEKGELCEEKLDFCSQDVNPCQHDSKC-ILTLKGYKCDCTPGYIGEHCDIDYDDCQENKCRNGARCVDAVNGYTCVCPEGYSGLFCE--SAPPMVLP-----------------------RTSPCDNFECQNGAQCIV----KGNEPQCQCLPGYHGNKCEKLVSVNFVNKDSFIQI-PSAK--IRTQANITLQVATDEDSGILLYKG-DKDQVAVELYRGRVRVSYDTG---INPASAIYSVETINDGNFHVVELVNLDQTLSLVVDGGTPKSITN--LPKQSLISIDSTLYIGGMPMKNNIAGLRQSSSQNGTNFLGCIRNLYINNELQDFRK-LNLQAGVLPGC 1357          

HSP 2 Score: 58.5362 bits (140), Expect = 1.057e-7
Identity = 43/115 (37.39%), Postives = 58/115 (50.43%), Query Frame = 1
Query: 2917 GICKNIDNYGNYECVCPVGFRGPKCTINVDD-CVGNLCVNGATCNDLNN-SYSCKCQSGFRGKYCDIKFLYCNDINPCQN----SGQCQLLPNNDYKCVCSKGWEGKQCERNTDD 3243
            G C      G + C C  G+ G  C I  +D C+GN CV+G TC  +N+ SYSCKC  GF G  CD      +  NPCQ+     G+C+L       C C+ G+ G  C++   D
Sbjct: 1368 GTCLATSQAG-FTCECEGGWTGQLCDIQSNDPCLGNKCVHG-TCLPINSFSYSCKCLQGFVGVLCDEPE---DQPNPCQSIKCKHGKCRLSGLGKAYCECNSGYTGDSCDKAFTD 1477          
BLAST of slit1 vs. Ensembl Mouse
Match: Slit3 (slit guidance ligand 3 [Source:MGI Symbol;Acc:MGI:1315202])

HSP 1 Score: 729.169 bits (1881), Expect = 0.000e+0
Identity = 468/1381 (33.89%), Postives = 709/1381 (51.34%), Query Frame = 1
Query:  148 SEICPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQLQ------------------------CINDNIFNKLGMLKYLLLEGNKIFWI-SEAISKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCASEDKANLENMCPSDKKVCSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQIYNFQC--NKEFESKYETHDEECVPKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLPPIYSIHEAPNLMKLDLS------------------------FNQIETLEPDVFTRSSKLREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQFHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCDGNASVYHSVDSCHETNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNE--TSFKNLLNLKTLV-LSYNGITELNKNVLTPLKSLETLVLSFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGN-YECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYC-NDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNSIYF-QKNSYIEISPPSRGFLMPRGNISLNFMTNSSSGLILYQGSEQTYLVVNLYQGHLRISYNLGDKVVGPEG--YSMGMVNDSRLHALHIEIVGENLTMYLNGRVHCTLKSKYLNSNRYMALDTGIFIGGAPHKILQSAIMKFHIETDSEFIGCMESLLINRKKIDFSKYLPKSINVHPGC 4113
            +  CP  C C   SV+C       VP  +     RL+L  N I  I K DF  L NL+VL L +N+++ IE GAF +L  L +LRLN+N +Q LP+ LF    KL +LDL +NQ+Q                        CI D  F  L  L+ L L  N I  I   + + +  ++ LR+H N L C+CHLAWL+ +L+Q+  I        LC  P  L    ++ +   E+ C           C ++   C   C+CS++ IV C GK L+ IP +LP  +VE+ L+ N+I  +  G  ++YK LK+I +++N + DI P AF GL  LTS+ L  N +  I K  F GLVSL  L L  N I CL  +T +  + L+LL L +NKL ++ K  F P  +++ L+L  NP  CDC + W+ DY++D    T  A C  PR L    +  ++   F+C  ++++ +++ +   EC   + CP KC C   +V+CSN  L  +P  +   T  L ++ N++  L       + PNL K++LS                         NQ+ET+   +F   S L+ + L SN + C++N+TF  L  + +L L +N I+ I   +F   ++L  + L  N F+C+C + W+  W R    V GN   P CQ P  LK+ PI  +  + F CDGN        SC  +      C+                    T V CS+  L  +P  +PKD TELYL+ NHL ++ +  ++F+ L    TL+ LS N I+ L  +  + +  L TL+LS+N+L+CI   AF  L +L+VL L  ND+S++P  +FNDL +L+++ALG NP HCDC+++WL+++    + + GI+ C+SPE M  + +  +    F C     D  I AKC++C+  PCK+NG CS      Y+C CPY + GK+C   IN C   PC +GG C   +N  + + C CP+GF G +C IN DDC  N C N ATC D  N+Y+C C   + G+ CD    YC  ++N CQ+  +C  L +  ++C C  G+ GK CE N DDC  + C+H +QCVD  N Y C+C  G+ G FCE P   P VL                       +  S C  +EC N   CI       Q+  C+C PG++G  C+ L ++ F  K+SY+E++      + P+ NISL   T+  +G++LY+G +   L + LYQGH+R+ Y   D +  P    YS+  VND + H++ + ++ + L + ++     +L    L     +  ++ +++GG P     SA+ +        F GC+  + IN +  DF    P+S+ V PGC
Sbjct:   31 AAACPTKCTCSAASVDCHGLGLRAVPRGIPRNAERLDLDRNNITRITKMDFAGLKNLRVLHLEDNQVSIIERGAFQDLKQLERLRLNKNKLQVLPELLFQSTPKLTRLDLSENQIQGIPRKAFRGVTGVKNLQLDNNHISCIEDGAFRALRDLEILTLNNNNISRILVTSFNHMPKIRTLRLHSNHLYCDCHLAWLSDWLRQRRTI----GQFTLCMAPVHLRGFSVADVQKKEYVCPGPHSE--APACNANSLSCPSACSCSNN-IVDCRGKGLTEIPANLPEGIVEIRLEQNSIKSIPAGAFTQYKKLKRIDISKNQISDIAPDAFQGLKSLTSLVLYGNKITEIPKGLFDGLVSLQLLLLNANKINCLRVNTFQDLQNLNLLSLYDNKLQTISKGLFVPLQSIQTLHLAQNPFVCDCHLKWLADYLQDNPIETSGARCSSPRRLANKRISQIKSKKFRCSGSEDYRNRFSS---ECFMDLVCPEKCRCEGTIVDCSNQKLARIPSHLPEYTTDLRLNDNDISVLEATGIFKKLPNLRKINLSNNRIKEVREGAFDGAAGVQELMLTGNQLETMHGRMFRGLSSLKTLMLRSNLISCVSNDTFAGLSSVRLLSLYDNRITTITPGAFTTLVSLSTINLLSNPFNCNCHMAWLGRWLRKRRIVSGN---PRCQKPFFLKEIPIQDVAIQDFTCDGNEE-----SSCQLSPRCPEQCTC-----------------VETVVRCSNRGLHALPKGMPKDVTELYLEGNHLTAVPKELSAFRQL----TLIDLSNNSISMLTNHTFSNMSHLSTLILSYNRLRCIPVHAFNGLRSLRVLTLHGNDISSVPEGSFNDLTSLSHLALGTNPLHCDCSLRWLSEWVKAGYKEPGIARCSSPESMADRLLLTTPTHRFQCKGPV-DINIVAKCNACLSSPCKNNGTCSQDPVEQYRCTCPYSYKGKDCTVPINTCVQNPCEHGGTCHLSENLRDGFSCSCPLGFEGQRCEINPDDCEDNDCENSATCVDGINNYACLCPPNYTGELCDEVIDYCVPEMNLCQHEAKCISL-DKGFRCECVPGYSGKLCETNNDDCVAHKCRHGAQCVDEVNGYTCICPQGFSGLFCEHPP--PMVL-----------------------LQTSPCDQYECQNGAQCI----VVQQEPTCRCPPGFAGPRCEKLITVNFVGKDSYVELASAK---VRPQANISLQVATDKDNGILLYKG-DNDPLALELYQGHVRLVY---DSLSSPPTTVYSVETVNDGQFHSVELVMLNQTLNLVVDKGAPKSLGK--LQKQPAVGSNSPLYLGGIPTSTGLSALRQGADRPLGGFHGCIHEVRINNELQDFKALPPQSLGVSPGC 1332          

HSP 2 Score: 186.422 bits (472), Expect = 9.019e-47
Identity = 128/479 (26.72%), Postives = 213/479 (44.47%), Query Frame = 1
Query: 1507 PKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLP-----------------------------------------------PIYSIHEAPNLMKLDLSFNQIETLEPDVFTRSSKLREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQFHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCDGNASVYHSVDSCHETNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVL------------------------SFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICS 2730
            P  +CP KCTCS+  V+C   GL ++P  I  + + L +  NN+ ++                                                P       P L +LDLS NQI+ +    F   + ++ + L++N + CI +  F+ L++LEIL L+ N IS I+  SF +   ++ L L+ N  +CDC L W+S+W R   T+    +   C +P+ L+   +  +  K ++C G  S   + ++   + S  + CS  N                   VDC    L EIP  +P+   E+ L++N ++S+   +F     LK + +S N I+++  +    LKSL +LVL                        + NK+ C+  + F++L NL +L L  N + TI    F  L+++  + L QNPF CDC++KWL  +  D  ++   + C+SP ++  K I   K   F CS
Sbjct:   30 PAAACPTKCTCSAASVDCHGLGLRAVPRGIPRNAERLDLDRNNITRITKMDFAGLKNLRVLHLEDNQVSIIERGAFQDLKQLERLRLNKNKLQVLPELLFQSTPKLTRLDLSENQIQGIPRKAFRGVTGVKNLQLDNNHISCIEDGAFRALRDLEILTLNNNNISRILVTSFNHMPKIRTLRLHSNHLYCDCHLAWLSDWLRQRRTI---GQFTLCMAPVHLRGFSVADVQKKEYVCPGPHSEAPACNA--NSLSCPSACSCSN-----------------NIVDCRGKGLTEIPANLPEGIVEIRLEQNSIKSIPAGAFTQYKKLKRIDISKNQISDIAPDAFQGLKSLTSLVLYGNKITEIPKGLFDGLVSLQLLLLNANKINCLRVNTFQDLQNLNLLSLYDNKLQTISKGLFVPLQSIQTLHLAQNPFVCDCHLKWLADYLQDNPIETSGARCSSPRRLANKRISQIKSKKFRCS 486          

HSP 3 Score: 56.225 bits (134), Expect = 4.913e-7
Identity = 39/115 (33.91%), Postives = 59/115 (51.30%), Query Frame = 1
Query: 2905 CRNGGICKNIDNYGNYECVCPVGFRGPKCTINV-DDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCND-INPCQ----NSGQCQLLPNNDYKCVCSKGWEGKQCER 3231
            CR+G +C++++   +  C C  G+ GP C     D C+G+ C +G TC    +SY CKC  G+ G  CD K    ND  + C     + GQC +    +  C+C  G+ G  CE+
Sbjct: 1338 CRHG-LCRSVEK-DSVVCECHPGWTGPLCDQEARDPCLGHSCRHG-TCMATGDSYVCKCAEGYGGALCDQK----NDSASACSAFKCHHGQCHISDRGEPYCLCQPGFSGHHCEQ 1445          

HSP 4 Score: 52.7582 bits (125), Expect = 5.665e-6
Identity = 39/141 (27.66%), Postives = 60/141 (42.55%), Query Frame = 1
Query: 3154 GQCQLLPNNDYKCVCSKGWEGKQCERNT-DDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLN 3573
            G C+ +  +   C C  GW G  C++   D C  +SC+H + C+   + Y C C  GY G  C+              + D                S S C+  +C ++G C   D+    + YC C+PG+SGH C+  N
Sbjct: 1341 GLCRSVEKDSVVCECHPGWTGPLCDQEARDPCLGHSCRHGT-CMATGDSYVCKCAEGYGGALCD-------------QKND----------------SASACSAFKC-HHGQCHISDRG---EPYCLCQPGFSGHHCEQEN 1447          
BLAST of slit1 vs. Ensembl Mouse
Match: Slit2 (slit guidance ligand 2 [Source:MGI Symbol;Acc:MGI:1315205])

HSP 1 Score: 702.59 bits (1812), Expect = 0.000e+0
Identity = 486/1483 (32.77%), Postives = 734/1483 (49.49%), Query Frame = 1
Query:  151 EICPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQLQ------------------------CINDNIFNKLGMLKYLLLEGNKIFWISEA-ISKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCASEDKANLENMCPSDKKV-CSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQIYNFQCN--KEFESKYETHDEECVPKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLPPIYSIHEAPNLMKLDLSFNQIETLEPDVFTRSSKLREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQ------------------------FHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCD-GNASVYHSVDSCHETNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVLSFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGIC--KNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYC-NDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNSIYF-QKNSYIEISPPSRGFLMPRGNISLNFMTNSSSGLILYQGSEQTYLVVNLYQGHLRISYNLGDKVVGPEGYSMGMVNDSRLHALHIEIVGENLTMYLNGRVHCTLKSKYLNSNRYMALDTGIFIGGAPHKILQSAIMKFHIETDSEFIGCMESLLINRKKIDFSKYLPKSINVHPGCGKLISTVVASDLKIPSIMXXXXXXXXXXXXXXXNNVCKRPGICKNQGVCEVLPGSKKKFRCICKNGFVGRKCKKRKSMCHGTFTESHIVDPDSQGTCKSIERFQY 4428
            + CP  C C   +V+C      +VP  +   T RL+L GN I  I K DF  L +L+VLQL  NRI++IE GAF +L  L +LRLNRN +Q  P+ LF    KL +LDL +NQ+Q                        CI D  F  L  L+ L L  N I  +S A  + +  L+  R+H N L C+CHLAWL+ +L+Q+  +   G ++  C  P  L    ++ +   EF C+ E++ +   M PS   + C   C CS++ IV C GK L+ IP +LP  + E+ L+ N+I ++  G  S YK L+++ L+ N + ++ P AF GL  L S+ L  N +  + K+ F GL SL  L L  N I CL  D  +    L+LL L +NKL ++ K TF     ++ ++L  NP  CDC + W+ DY+      T  A C  PR L    +  ++   F+C+  +++ SK      +C   ++CP KC C    V+CSN  L  +P+ I   T  L +++N    L       + P L K++ S N+I  +E   F  +S + EI L SNRL+ + ++ FK L+ L+ L L  N ISC+  DSF    +++ L L  NQ                        F+C+C L W+ EW R    V GN   P CQ P  LK+ PI  +  + F CD GN       +SC   +   + C+                    T V CS+  LK +P  IPKD TELYLD N   +L      N  +L  + LS N I+ L+    + +  L TL+LS+N+L+CI    F  L +L++L L  ND+S +P  AFNDL  L+++A+G NP +CDCN++WL+ +    + + GI+ CA P +M  K +  +    F C     D  I AKC+ C+  PCK++G C+      Y+C CPY F G++CD  I+AC   PC++GG C  K  +N G + C C  GF G  C +N+DDC  N C N +TC D  N+Y+C C   + G+ C+ K  +C  D+NPCQ+  +C L P   +KC C+ G+ G+ C+ + DDC+ N CK+ + C D  N Y C+C  GY G FCE     P VL                          S C   +C N   CI     +  +  C+C PGY G  C+ L S+ F  K SY++I P ++  + P+ NI+L   T+  SG++LY+G ++ ++ V LY+G +R SY+ G        YS+  +ND   H + +  +  +L++ ++G     + +  L+    +  D+ +++GG P K   +++ +   +  + F GC+ +L IN +  DF K +P    + PGC      V A                                     G+C+  P S+  F C C+ G++G  C +R +       +  + +    GTC  I  F Y
Sbjct:   26 QACPAQCSCSGSTVDCHGLALRSVPRNIPRNTERLDLNGNNITRITKIDFAGLRHLRVLQLMENRISTIERGAFQDLKELERLRLNRNNLQLFPELLFLGTAKLYRLDLSENQIQAIPRKAFRGAVDIKNLQLDYNQISCIEDGAFRALRDLEVLTLNNNNITRLSVASFNHMPKLRTFRLHSNNLYCDCHLAWLSDWLRQRPRV---GLYTQ-CMGPSHLRGHNVAEVQKREFVCSDEEEGHQSFMAPSCSVLHCPAACTCSNN-IVDCRGKGLTEIPTNLPETITEIRLEQNSIRVIPPGAFSPYKKLRRLDLSNNQISELAPDAFQGLRSLNSLVLYGNKITELPKSLFEGLFSLQLLLLNANKINCLRVDAFQDLHNLNLLSLYDNKLQTVAKGTFSALRAIQTMHLAQNPFICDCHLKWLADYLHTNPIETSGARCTSPRRLANKRIGQIKSKKFRCSGTEDYRSKLSG---DCFADLACPEKCRCEGTTVDCSNQRLNKIPDHIPQYTAELRLNNNEFTVLEATGIFKKLPQLRKINFSNNKITDIEEGAFEGASGVNEILLTSNRLENVQHKMFKGLESLKTLMLRSNRISCVGNDSFIGLGSVRLLSLYDNQITTVAPGAFDSLHSLSTLNLLANPFNCNCHLAWLGEWLRRKRIVTGN---PRCQKPYFLKEIPIQDVAIQDFTCDDGNDD-----NSCSPLSRCPSECTC-----------------LDTVVRCSNKGLKVLPKGIPKDVTELYLDGNQF-TLVPKELSNYKHLTLIDLSNNRISTLSNQSFSNMTQLLTLILSYNRLRCIPPRTFDGLKSLRLLSLHGNDISVVPEGAFNDLSALSHLAIGANPLYCDCNMQWLSDWVKSEYKEPGIARCAGPGEMADKLLLTTPSKKFTCQGPV-DITIQAKCNPCLSNPCKNDGTCNNDPVDFYRCTCPYGFKGQDCDVPIHACISNPCKHGGTCHLKEGENAG-FWCTCADGFEGENCEVNIDDCEDNDCENNSTCVDGINNYTCLCPPEYTGELCEEKLDFCAQDLNPCQHDSKCILTPKG-FKCDCTPGYIGEHCDIDFDDCQDNKCKNGAHCTDAVNGYTCVCPEGYSGLFCEFSP--PMVLP-----------------------RTSPCDNFDCQNGAQCI----IRINEPICQCLPGYLGEKCEKLVSVNFVNKESYLQI-PSAK--VRPQTNITLQIATDEDSGILLYKG-DKDHIAVELYRGRVRASYDTGSHPASAI-YSVETINDGNFHIVELLTLDSSLSLSVDGGSPKVITN--LSKQSTLNFDSPLYVGGMPGKNNVASLRQAPGQNGTSFHGCIRNLYINSELQDFRK-MPMQTGILPGCEPCHKKVCA------------------------------------HGMCQ--PSSQSGFTCECEEGWMGPLCDQRTN-------DPCLGNKCVHGTCLPINAFSY 1389          

HSP 2 Score: 57.7658 bits (138), Expect = 1.548e-7
Identity = 42/111 (37.84%), Postives = 57/111 (51.35%), Query Frame = 1
Query: 2917 GICKNIDNYGNYECVCPVGFRGPKCTINVDD-CVGNLCVNGATCNDLNN-SYSCKCQSGFRGKYCDIKFLYCNDINPCQ----NSGQCQLLPNNDYKCVCSKGWEGKQCER 3231
            G+C+     G + C C  G+ GP C    +D C+GN CV+G TC  +N  SYSCKC  G  G  CD +    +  NPCQ      G+C+L       C C+ G+ G  C+R
Sbjct: 1340 GMCQPSSQSG-FTCECEEGWMGPLCDQRTNDPCLGNKCVHG-TCLPINAFSYSCKCLEGHGGVLCDEEE---DLFNPCQMIKCKHGKCRLSGVGQPYCECNSGFTGDSCDR 1445          
BLAST of slit1 vs. Ensembl Mouse
Match: Slit2 (slit guidance ligand 2 [Source:MGI Symbol;Acc:MGI:1315205])

HSP 1 Score: 699.508 bits (1804), Expect = 0.000e+0
Identity = 486/1491 (32.60%), Postives = 734/1491 (49.23%), Query Frame = 1
Query:  151 EICPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQLQ------------------------CINDNIFNKLGMLKYLLLEGNKIFWISEA-ISKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCASEDKANLENMCPSDKKV-CSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQIYNFQCN----------KEFESKYETHDEECVPKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLPPIYSIHEAPNLMKLDLSFNQIETLEPDVFTRSSKLREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQ------------------------FHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCD-GNASVYHSVDSCHETNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVLSFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGIC--KNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYC-NDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNSIYF-QKNSYIEISPPSRGFLMPRGNISLNFMTNSSSGLILYQGSEQTYLVVNLYQGHLRISYNLGDKVVGPEGYSMGMVNDSRLHALHIEIVGENLTMYLNGRVHCTLKSKYLNSNRYMALDTGIFIGGAPHKILQSAIMKFHIETDSEFIGCMESLLINRKKIDFSKYLPKSINVHPGCGKLISTVVASDLKIPSIMXXXXXXXXXXXXXXXNNVCKRPGICKNQGVCEVLPGSKKKFRCICKNGFVGRKCKKRKSMCHGTFTESHIVDPDSQGTCKSIERFQY 4428
            + CP  C C   +V+C      +VP  +   T RL+L GN I  I K DF  L +L+VLQL  NRI++IE GAF +L  L +LRLNRN +Q  P+ LF    KL +LDL +NQ+Q                        CI D  F  L  L+ L L  N I  +S A  + +  L+  R+H N L C+CHLAWL+ +L+Q+  +   G ++  C  P  L    ++ +   EF C+ E++ +   M PS   + C   C CS++ IV C GK L+ IP +LP  + E+ L+ N+I ++  G  S YK L+++ L+ N + ++ P AF GL  L S+ L  N +  + K+ F GL SL  L L  N I CL  D  +    L+LL L +NKL ++ K TF     ++ ++L  NP  CDC + W+ DY+      T  A C  PR L    +  ++   F+C+          +++ SK      +C   ++CP KC C    V+CSN  L  +P+ I   T  L +++N    L       + P L K++ S N+I  +E   F  +S + EI L SNRL+ + ++ FK L+ L+ L L  N ISC+  DSF    +++ L L  NQ                        F+C+C L W+ EW R    V GN   P CQ P  LK+ PI  +  + F CD GN       +SC   +   + C+                    T V CS+  LK +P  IPKD TELYLD N   +L      N  +L  + LS N I+ L+    + +  L TL+LS+N+L+CI    F  L +L++L L  ND+S +P  AFNDL  L+++A+G NP +CDCN++WL+ +    + + GI+ CA P +M  K +  +    F C     D  I AKC+ C+  PCK++G C+      Y+C CPY F G++CD  I+AC   PC++GG C  K  +N G + C C  GF G  C +N+DDC  N C N +TC D  N+Y+C C   + G+ C+ K  +C  D+NPCQ+  +C L P   +KC C+ G+ G+ C+ + DDC+ N CK+ + C D  N Y C+C  GY G FCE     P VL                          S C   +C N   CI     +  +  C+C PGY G  C+ L S+ F  K SY++I P ++  + P+ NI+L   T+  SG++LY+G ++ ++ V LY+G +R SY+ G        YS+  +ND   H + +  +  +L++ ++G     + +  L+    +  D+ +++GG P K   +++ +   +  + F GC+ +L IN +  DF K +P    + PGC      V A                                     G+C+  P S+  F C C+ G++G  C +R +       +  + +    GTC  I  F Y
Sbjct:   26 QACPAQCSCSGSTVDCHGLALRSVPRNIPRNTERLDLNGNNITRITKIDFAGLRHLRVLQLMENRISTIERGAFQDLKELERLRLNRNNLQLFPELLFLGTAKLYRLDLSENQIQAIPRKAFRGAVDIKNLQLDYNQISCIEDGAFRALRDLEVLTLNNNNITRLSVASFNHMPKLRTFRLHSNNLYCDCHLAWLSDWLRQRPRV---GLYTQ-CMGPSHLRGHNVAEVQKREFVCSDEEEGHQSFMAPSCSVLHCPAACTCSNN-IVDCRGKGLTEIPTNLPETITEIRLEQNSIRVIPPGAFSPYKKLRRLDLSNNQISELAPDAFQGLRSLNSLVLYGNKITELPKSLFEGLFSLQLLLLNANKINCLRVDAFQDLHNLNLLSLYDNKLQTVAKGTFSALRAIQTMHLAQNPFICDCHLKWLADYLHTNPIETSGARCTSPRRLANKRIGQIKSKKFRCSAKEQYFIPGTEDYRSKLSG---DCFADLACPEKCRCEGTTVDCSNQRLNKIPDHIPQYTAELRLNNNEFTVLEATGIFKKLPQLRKINFSNNKITDIEEGAFEGASGVNEILLTSNRLENVQHKMFKGLESLKTLMLRSNRISCVGNDSFIGLGSVRLLSLYDNQITTVAPGAFDSLHSLSTLNLLANPFNCNCHLAWLGEWLRRKRIVTGN---PRCQKPYFLKEIPIQDVAIQDFTCDDGNDD-----NSCSPLSRCPSECTC-----------------LDTVVRCSNKGLKVLPKGIPKDVTELYLDGNQF-TLVPKELSNYKHLTLIDLSNNRISTLSNQSFSNMTQLLTLILSYNRLRCIPPRTFDGLKSLRLLSLHGNDISVVPEGAFNDLSALSHLAIGANPLYCDCNMQWLSDWVKSEYKEPGIARCAGPGEMADKLLLTTPSKKFTCQGPV-DITIQAKCNPCLSNPCKNDGTCNNDPVDFYRCTCPYGFKGQDCDVPIHACISNPCKHGGTCHLKEGENAG-FWCTCADGFEGENCEVNIDDCEDNDCENNSTCVDGINNYTCLCPPEYTGELCEEKLDFCAQDLNPCQHDSKCILTPKG-FKCDCTPGYIGEHCDIDFDDCQDNKCKNGAHCTDAVNGYTCVCPEGYSGLFCEFSP--PMVLP-----------------------RTSPCDNFDCQNGAQCI----IRINEPICQCLPGYLGEKCEKLVSVNFVNKESYLQI-PSAK--VRPQTNITLQIATDEDSGILLYKG-DKDHIAVELYRGRVRASYDTGSHPASAI-YSVETINDGNFHIVELLTLDSSLSLSVDGGSPKVITN--LSKQSTLNFDSPLYVGGMPGKNNVASLRQAPGQNGTSFHGCIRNLYINSELQDFRK-MPMQTGILPGCEPCHKKVCA------------------------------------HGMCQ--PSSQSGFTCECEEGWMGPLCDQRTN-------DPCLGNKCVHGTCLPINAFSY 1397          

HSP 2 Score: 57.7658 bits (138), Expect = 1.536e-7
Identity = 42/111 (37.84%), Postives = 57/111 (51.35%), Query Frame = 1
Query: 2917 GICKNIDNYGNYECVCPVGFRGPKCTINVDD-CVGNLCVNGATCNDLNN-SYSCKCQSGFRGKYCDIKFLYCNDINPCQ----NSGQCQLLPNNDYKCVCSKGWEGKQCER 3231
            G+C+     G + C C  G+ GP C    +D C+GN CV+G TC  +N  SYSCKC  G  G  CD +    +  NPCQ      G+C+L       C C+ G+ G  C+R
Sbjct: 1348 GMCQPSSQSG-FTCECEEGWMGPLCDQRTNDPCLGNKCVHG-TCLPINAFSYSCKCLEGHGGVLCDEEE---DLFNPCQMIKCKHGKCRLSGVGQPYCECNSGFTGDSCDR 1453          
BLAST of slit1 vs. Ensembl Mouse
Match: Slit2 (slit guidance ligand 2 [Source:MGI Symbol;Acc:MGI:1315205])

HSP 1 Score: 695.271 bits (1793), Expect = 0.000e+0
Identity = 485/1483 (32.70%), Postives = 730/1483 (49.22%), Query Frame = 1
Query:  151 EICPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQLQ------------------------CINDNIFNKLGMLKYLLLEGNKIFWISEA-ISKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCASEDKANLENMCPSDKKV-CSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQIYNFQCN--KEFESKYETHDEECVPKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLPPIYSIHEAPNLMKLDLSFNQIETLEPDVFTRSSKLREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQ------------------------FHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCD-GNASVYHSVDSCHETNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVLSFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGIC--KNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYC-NDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNSIYF-QKNSYIEISPPSRGFLMPRGNISLNFMTNSSSGLILYQGSEQTYLVVNLYQGHLRISYNLGDKVVGPEGYSMGMVNDSRLHALHIEIVGENLTMYLNGRVHCTLKSKYLNSNRYMALDTGIFIGGAPHKILQSAIMKFHIETDSEFIGCMESLLINRKKIDFSKYLPKSINVHPGCGKLISTVVASDLKIPSIMXXXXXXXXXXXXXXXNNVCKRPGICKNQGVCEVLPGSKKKFRCICKNGFVGRKCKKRKSMCHGTFTESHIVDPDSQGTCKSIERFQY 4428
            + CP  C C   +V+C      +VP  +   T RL+L GN I  I K DF  L +L+VLQL  NRI++IE GAF +L  L +LRLNRN +Q  P+ LF    KL +LDL +NQ+Q                        CI D  F  L  L+ L L  N I  +S A  + +  L+  R+H N L C+CHLAWL+ +L+Q+  +   G ++  C  P  L    ++ +   EF C+         M PS   + C   C CS++ IV C GK L+ IP +LP  + E+ L+ N+I ++  G  S YK L+++ L+ N + ++ P AF GL  L S+ L  N +  + K+ F GL SL  L L  N I CL  D  +    L+LL L +NKL ++ K TF     ++ ++L  NP  CDC + W+ DY+      T  A C  PR L    +  ++   F+C+  +++ SK      +C   ++CP KC C    V+CSN  L  +P+ I   T  L +++N    L       + P L K++ S N+I  +E   F  +S + EI L SNRL+ + ++ FK L+ L+ L L  N ISC+  DSF    +++ L L  NQ                        F+C+C L W+ EW R    V GN   P CQ P  LK+ PI  +  + F CD GN       +SC   +   + C+                    T V CS+  LK +P  IPKD TELYLD N   +L      N  +L  + LS N I+ L+    + +  L TL+LS+N+L+CI    F  L +L++L L  ND+S +P  AFNDL  L+++A+G NP +CDCN++WL+ +    + + GI+ CA P +M  K +  +    F C     D  I AKC+ C+  PCK++G C+      Y+C CPY F G++CD  I+AC   PC++GG C  K  +N G + C C  GF G  C +N+DDC  N C N +TC D  N+Y+C C   + G+ C+ K  +C  D+NPCQ+  +C L P   +KC C+ G+ G+ C+ + DDC+ N CK+ + C D  N Y C+C  GY G FCE     P VL                          S C   +C N   CI     +  +  C+C PGY G  C+ L S+ F  K SY++I P ++  + P+ NI+L   T+  SG++LY+G ++ ++ V LY+G +R SY+ G        YS+  +ND   H + +  +  +L++ ++G     + +  L+    +  D+ +++GG P K   +++ +   +  + F GC+ +L IN +  DF K +P    + PGC      V A                                     G+C+  P S+  F C C+ G++G  C +R +       +  + +    GTC  I  F Y
Sbjct:   26 QACPAQCSCSGSTVDCHGLALRSVPRNIPRNTERLDLNGNNITRITKIDFAGLRHLRVLQLMENRISTIERGAFQDLKELERLRLNRNNLQLFPELLFLGTAKLYRLDLSENQIQAIPRKAFRGAVDIKNLQLDYNQISCIEDGAFRALRDLEVLTLNNNNITRLSVASFNHMPKLRTFRLHSNNLYCDCHLAWLSDWLRQRPRV---GLYTQ-CMGPSHLRGHNVAEVQKREFVCSGHQSF----MAPSCSVLHCPAACTCSNN-IVDCRGKGLTEIPTNLPETITEIRLEQNSIRVIPPGAFSPYKKLRRLDLSNNQISELAPDAFQGLRSLNSLVLYGNKITELPKSLFEGLFSLQLLLLNANKINCLRVDAFQDLHNLNLLSLYDNKLQTVAKGTFSALRAIQTMHLAQNPFICDCHLKWLADYLHTNPIETSGARCTSPRRLANKRIGQIKSKKFRCSGTEDYRSKLSG---DCFADLACPEKCRCEGTTVDCSNQRLNKIPDHIPQYTAELRLNNNEFTVLEATGIFKKLPQLRKINFSNNKITDIEEGAFEGASGVNEILLTSNRLENVQHKMFKGLESLKTLMLRSNRISCVGNDSFIGLGSVRLLSLYDNQITTVAPGAFDSLHSLSTLNLLANPFNCNCHLAWLGEWLRRKRIVTGN---PRCQKPYFLKEIPIQDVAIQDFTCDDGNDD-----NSCSPLSRCPSECTC-----------------LDTVVRCSNKGLKVLPKGIPKDVTELYLDGNQF-TLVPKELSNYKHLTLIDLSNNRISTLSNQSFSNMTQLLTLILSYNRLRCIPPRTFDGLKSLRLLSLHGNDISVVPEGAFNDLSALSHLAIGANPLYCDCNMQWLSDWVKSEYKEPGIARCAGPGEMADKLLLTTPSKKFTCQGPV-DITIQAKCNPCLSNPCKNDGTCNNDPVDFYRCTCPYGFKGQDCDVPIHACISNPCKHGGTCHLKEGENAG-FWCTCADGFEGENCEVNIDDCEDNDCENNSTCVDGINNYTCLCPPEYTGELCEEKLDFCAQDLNPCQHDSKCILTPKG-FKCDCTPGYIGEHCDIDFDDCQDNKCKNGAHCTDAVNGYTCVCPEGYSGLFCEFSP--PMVLP-----------------------RTSPCDNFDCQNGAQCI----IRINEPICQCLPGYLGEKCEKLVSVNFVNKESYLQI-PSAK--VRPQTNITLQIATDEDSGILLYKG-DKDHIAVELYRGRVRASYDTGSHPASAI-YSVETINDGNFHIVELLTLDSSLSLSVDGGSPKVITN--LSKQSTLNFDSPLYVGGMPGKNNVASLRQAPGQNGTSFHGCIRNLYINSELQDFRK-MPMQTGILPGCEPCHKKVCA------------------------------------HGMCQ--PSSQSGFTCECEEGWMGPLCDQRTN-------DPCLGNKCVHGTCLPINAFSY 1385          

HSP 2 Score: 57.7658 bits (138), Expect = 1.534e-7
Identity = 42/111 (37.84%), Postives = 57/111 (51.35%), Query Frame = 1
Query: 2917 GICKNIDNYGNYECVCPVGFRGPKCTINVDD-CVGNLCVNGATCNDLNN-SYSCKCQSGFRGKYCDIKFLYCNDINPCQ----NSGQCQLLPNNDYKCVCSKGWEGKQCER 3231
            G+C+     G + C C  G+ GP C    +D C+GN CV+G TC  +N  SYSCKC  G  G  CD +    +  NPCQ      G+C+L       C C+ G+ G  C+R
Sbjct: 1336 GMCQPSSQSG-FTCECEEGWMGPLCDQRTNDPCLGNKCVHG-TCLPINAFSYSCKCLEGHGGVLCDEEE---DLFNPCQMIKCKHGKCRLSGVGQPYCECNSGFTGDSCDR 1441          
BLAST of slit1 vs. Ensembl Mouse
Match: Slit2 (slit guidance ligand 2 [Source:MGI Symbol;Acc:MGI:1315205])

HSP 1 Score: 692.575 bits (1786), Expect = 0.000e+0
Identity = 486/1500 (32.40%), Postives = 735/1500 (49.00%), Query Frame = 1
Query:  151 EICPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQLQ------------------------CINDNIFNKLGMLKYLLLEGNKIFWISEA-ISKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCASEDKANLENMCPSDKKV-CSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQIYNFQCN----------KEFESKYETHDEECVPKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLPPIYSIHEAPNLMKLDLSFNQIETLEPDVFTRSSKLREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQ------------------------FHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCD-GNASVYHSVDSCHETNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVLSFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGIC--KNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGF---------RGKYCDIKFLYC-NDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNSIYF-QKNSYIEISPPSRGFLMPRGNISLNFMTNSSSGLILYQGSEQTYLVVNLYQGHLRISYNLGDKVVGPEGYSMGMVNDSRLHALHIEIVGENLTMYLNGRVHCTLKSKYLNSNRYMALDTGIFIGGAPHKILQSAIMKFHIETDSEFIGCMESLLINRKKIDFSKYLPKSINVHPGCGKLISTVVASDLKIPSIMXXXXXXXXXXXXXXXNNVCKRPGICKNQGVCEVLPGSKKKFRCICKNGFVGRKCKKRKSMCHGTFTESHIVDPDSQGTCKSIERFQY 4428
            + CP  C C   +V+C      +VP  +   T RL+L GN I  I K DF  L +L+VLQL  NRI++IE GAF +L  L +LRLNRN +Q  P+ LF    KL +LDL +NQ+Q                        CI D  F  L  L+ L L  N I  +S A  + +  L+  R+H N L C+CHLAWL+ +L+Q+  +   G ++  C  P  L    ++ +   EF C+ E++ +   M PS   + C   C CS++ IV C GK L+ IP +LP  + E+ L+ N+I ++  G  S YK L+++ L+ N + ++ P AF GL  L S+ L  N +  + K+ F GL SL  L L  N I CL  D  +    L+LL L +NKL ++ K TF     ++ ++L  NP  CDC + W+ DY+      T  A C  PR L    +  ++   F+C+          +++ SK      +C   ++CP KC C    V+CSN  L  +P+ I   T  L +++N    L       + P L K++ S N+I  +E   F  +S + EI L SNRL+ + ++ FK L+ L+ L L  N ISC+  DSF    +++ L L  NQ                        F+C+C L W+ EW R    V GN   P CQ P  LK+ PI  +  + F CD GN       +SC   +   + C+                    T V CS+  LK +P  IPKD TELYLD N   +L      N  +L  + LS N I+ L+    + +  L TL+LS+N+L+CI    F  L +L++L L  ND+S +P  AFNDL  L+++A+G NP +CDCN++WL+ +    + + GI+ CA P +M  K +  +    F C     D  I AKC+ C+  PCK++G C+      Y+C CPY F G++CD  I+AC   PC++GG C  K  +N G + C C  GF G  C +N+DDC  N C N +TC D  N+Y+C C   +         +G+ C+ K  +C  D+NPCQ+  +C L P   +KC C+ G+ G+ C+ + DDC+ N CK+ + C D  N Y C+C  GY G FCE     P VL                          S C   +C N   CI     +  +  C+C PGY G  C+ L S+ F  K SY++I P ++  + P+ NI+L   T+  SG++LY+G ++ ++ V LY+G +R SY+ G        YS+  +ND   H + +  +  +L++ ++G     + +  L+    +  D+ +++GG P K   +++ +   +  + F GC+ +L IN +  DF K +P    + PGC      V A                                     G+C+  P S+  F C C+ G++G  C +R +       +  + +    GTC  I  F Y
Sbjct:   26 QACPAQCSCSGSTVDCHGLALRSVPRNIPRNTERLDLNGNNITRITKIDFAGLRHLRVLQLMENRISTIERGAFQDLKELERLRLNRNNLQLFPELLFLGTAKLYRLDLSENQIQAIPRKAFRGAVDIKNLQLDYNQISCIEDGAFRALRDLEVLTLNNNNITRLSVASFNHMPKLRTFRLHSNNLYCDCHLAWLSDWLRQRPRV---GLYTQ-CMGPSHLRGHNVAEVQKREFVCSDEEEGHQSFMAPSCSVLHCPAACTCSNN-IVDCRGKGLTEIPTNLPETITEIRLEQNSIRVIPPGAFSPYKKLRRLDLSNNQISELAPDAFQGLRSLNSLVLYGNKITELPKSLFEGLFSLQLLLLNANKINCLRVDAFQDLHNLNLLSLYDNKLQTVAKGTFSALRAIQTMHLAQNPFICDCHLKWLADYLHTNPIETSGARCTSPRRLANKRIGQIKSKKFRCSAKEQYFIPGTEDYRSKLSG---DCFADLACPEKCRCEGTTVDCSNQRLNKIPDHIPQYTAELRLNNNEFTVLEATGIFKKLPQLRKINFSNNKITDIEEGAFEGASGVNEILLTSNRLENVQHKMFKGLESLKTLMLRSNRISCVGNDSFIGLGSVRLLSLYDNQITTVAPGAFDSLHSLSTLNLLANPFNCNCHLAWLGEWLRRKRIVTGN---PRCQKPYFLKEIPIQDVAIQDFTCDDGNDD-----NSCSPLSRCPSECTC-----------------LDTVVRCSNKGLKVLPKGIPKDVTELYLDGNQF-TLVPKELSNYKHLTLIDLSNNRISTLSNQSFSNMTQLLTLILSYNRLRCIPPRTFDGLKSLRLLSLHGNDISVVPEGAFNDLSALSHLAIGANPLYCDCNMQWLSDWVKSEYKEPGIARCAGPGEMADKLLLTTPSKKFTCQGPV-DITIQAKCNPCLSNPCKNDGTCNNDPVDFYRCTCPYGFKGQDCDVPIHACISNPCKHGGTCHLKEGENAG-FWCTCADGFEGENCEVNIDDCEDNDCENNSTCVDGINNYTCLCPPEYTAANLNEVEKGELCEEKLDFCAQDLNPCQHDSKCILTPKG-FKCDCTPGYIGEHCDIDFDDCQDNKCKNGAHCTDAVNGYTCVCPEGYSGLFCEFSP--PMVLP-----------------------RTSPCDNFDCQNGAQCI----IRINEPICQCLPGYLGEKCEKLVSVNFVNKESYLQI-PSAK--VRPQTNITLQIATDEDSGILLYKG-DKDHIAVELYRGRVRASYDTGSHPASAI-YSVETINDGNFHIVELLTLDSSLSLSVDGGSPKVITN--LSKQSTLNFDSPLYVGGMPGKNNVASLRQAPGQNGTSFHGCIRNLYINSELQDFRK-MPMQTGILPGCEPCHKKVCA------------------------------------HGMCQ--PSSQSGFTCECEEGWMGPLCDQRTN-------DPCLGNKCVHGTCLPINAFSY 1406          

HSP 2 Score: 57.7658 bits (138), Expect = 1.462e-7
Identity = 42/111 (37.84%), Postives = 57/111 (51.35%), Query Frame = 1
Query: 2917 GICKNIDNYGNYECVCPVGFRGPKCTINVDD-CVGNLCVNGATCNDLNN-SYSCKCQSGFRGKYCDIKFLYCNDINPCQ----NSGQCQLLPNNDYKCVCSKGWEGKQCER 3231
            G+C+     G + C C  G+ GP C    +D C+GN CV+G TC  +N  SYSCKC  G  G  CD +    +  NPCQ      G+C+L       C C+ G+ G  C+R
Sbjct: 1357 GMCQPSSQSG-FTCECEEGWMGPLCDQRTNDPCLGNKCVHG-TCLPINAFSYSCKCLEGHGGVLCDEEE---DLFNPCQMIKCKHGKCRLSGVGQPYCECNSGFTGDSCDR 1462          
BLAST of slit1 vs. UniProt/SwissProt
Match: sp|Q9WVB4|SLIT3_MOUSE (Slit homolog 3 protein OS=Mus musculus OX=10090 GN=Slit3 PE=2 SV=2)

HSP 1 Score: 729.169 bits (1881), Expect = 0.000e+0
Identity = 468/1381 (33.89%), Postives = 709/1381 (51.34%), Query Frame = 1
Query:  148 SEICPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQLQ------------------------CINDNIFNKLGMLKYLLLEGNKIFWI-SEAISKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCASEDKANLENMCPSDKKVCSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQIYNFQC--NKEFESKYETHDEECVPKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLPPIYSIHEAPNLMKLDLS------------------------FNQIETLEPDVFTRSSKLREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQFHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCDGNASVYHSVDSCHETNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNE--TSFKNLLNLKTLV-LSYNGITELNKNVLTPLKSLETLVLSFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGN-YECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYC-NDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNSIYF-QKNSYIEISPPSRGFLMPRGNISLNFMTNSSSGLILYQGSEQTYLVVNLYQGHLRISYNLGDKVVGPEG--YSMGMVNDSRLHALHIEIVGENLTMYLNGRVHCTLKSKYLNSNRYMALDTGIFIGGAPHKILQSAIMKFHIETDSEFIGCMESLLINRKKIDFSKYLPKSINVHPGC 4113
            +  CP  C C   SV+C       VP  +     RL+L  N I  I K DF  L NL+VL L +N+++ IE GAF +L  L +LRLN+N +Q LP+ LF    KL +LDL +NQ+Q                        CI D  F  L  L+ L L  N I  I   + + +  ++ LR+H N L C+CHLAWL+ +L+Q+  I        LC  P  L    ++ +   E+ C           C ++   C   C+CS++ IV C GK L+ IP +LP  +VE+ L+ N+I  +  G  ++YK LK+I +++N + DI P AF GL  LTS+ L  N +  I K  F GLVSL  L L  N I CL  +T +  + L+LL L +NKL ++ K  F P  +++ L+L  NP  CDC + W+ DY++D    T  A C  PR L    +  ++   F+C  ++++ +++ +   EC   + CP KC C   +V+CSN  L  +P  +   T  L ++ N++  L       + PNL K++LS                         NQ+ET+   +F   S L+ + L SN + C++N+TF  L  + +L L +N I+ I   +F   ++L  + L  N F+C+C + W+  W R    V GN   P CQ P  LK+ PI  +  + F CDGN        SC  +      C+                    T V CS+  L  +P  +PKD TELYL+ NHL ++ +  ++F+ L    TL+ LS N I+ L  +  + +  L TL+LS+N+L+CI   AF  L +L+VL L  ND+S++P  +FNDL +L+++ALG NP HCDC+++WL+++    + + GI+ C+SPE M  + +  +    F C     D  I AKC++C+  PCK+NG CS      Y+C CPY + GK+C   IN C   PC +GG C   +N  + + C CP+GF G +C IN DDC  N C N ATC D  N+Y+C C   + G+ CD    YC  ++N CQ+  +C  L +  ++C C  G+ GK CE N DDC  + C+H +QCVD  N Y C+C  G+ G FCE P   P VL                       +  S C  +EC N   CI       Q+  C+C PG++G  C+ L ++ F  K+SY+E++      + P+ NISL   T+  +G++LY+G +   L + LYQGH+R+ Y   D +  P    YS+  VND + H++ + ++ + L + ++     +L    L     +  ++ +++GG P     SA+ +        F GC+  + IN +  DF    P+S+ V PGC
Sbjct:   31 AAACPTKCTCSAASVDCHGLGLRAVPRGIPRNAERLDLDRNNITRITKMDFAGLKNLRVLHLEDNQVSIIERGAFQDLKQLERLRLNKNKLQVLPELLFQSTPKLTRLDLSENQIQGIPRKAFRGVTGVKNLQLDNNHISCIEDGAFRALRDLEILTLNNNNISRILVTSFNHMPKIRTLRLHSNHLYCDCHLAWLSDWLRQRRTI----GQFTLCMAPVHLRGFSVADVQKKEYVCPGPHSE--APACNANSLSCPSACSCSNN-IVDCRGKGLTEIPANLPEGIVEIRLEQNSIKSIPAGAFTQYKKLKRIDISKNQISDIAPDAFQGLKSLTSLVLYGNKITEIPKGLFDGLVSLQLLLLNANKINCLRVNTFQDLQNLNLLSLYDNKLQTISKGLFVPLQSIQTLHLAQNPFVCDCHLKWLADYLQDNPIETSGARCSSPRRLANKRISQIKSKKFRCSGSEDYRNRFSS---ECFMDLVCPEKCRCEGTIVDCSNQKLARIPSHLPEYTTDLRLNDNDISVLEATGIFKKLPNLRKINLSNNRIKEVREGAFDGAAGVQELMLTGNQLETMHGRMFRGLSSLKTLMLRSNLISCVSNDTFAGLSSVRLLSLYDNRITTITPGAFTTLVSLSTINLLSNPFNCNCHMAWLGRWLRKRRIVSGN---PRCQKPFFLKEIPIQDVAIQDFTCDGNEE-----SSCQLSPRCPEQCTC-----------------VETVVRCSNRGLHALPKGMPKDVTELYLEGNHLTAVPKELSAFRQL----TLIDLSNNSISMLTNHTFSNMSHLSTLILSYNRLRCIPVHAFNGLRSLRVLTLHGNDISSVPEGSFNDLTSLSHLALGTNPLHCDCSLRWLSEWVKAGYKEPGIARCSSPESMADRLLLTTPTHRFQCKGPV-DINIVAKCNACLSSPCKNNGTCSQDPVEQYRCTCPYSYKGKDCTVPINTCVQNPCEHGGTCHLSENLRDGFSCSCPLGFEGQRCEINPDDCEDNDCENSATCVDGINNYACLCPPNYTGELCDEVIDYCVPEMNLCQHEAKCISL-DKGFRCECVPGYSGKLCETNNDDCVAHKCRHGAQCVDEVNGYTCICPQGFSGLFCEHPP--PMVL-----------------------LQTSPCDQYECQNGAQCI----VVQQEPTCRCPPGFAGPRCEKLITVNFVGKDSYVELASAK---VRPQANISLQVATDKDNGILLYKG-DNDPLALELYQGHVRLVY---DSLSSPPTTVYSVETVNDGQFHSVELVMLNQTLNLVVDKGAPKSLGK--LQKQPAVGSNSPLYLGGIPTSTGLSALRQGADRPLGGFHGCIHEVRINNELQDFKALPPQSLGVSPGC 1332          

HSP 2 Score: 186.422 bits (472), Expect = 6.309e-46
Identity = 128/479 (26.72%), Postives = 213/479 (44.47%), Query Frame = 1
Query: 1507 PKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLP-----------------------------------------------PIYSIHEAPNLMKLDLSFNQIETLEPDVFTRSSKLREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQFHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCDGNASVYHSVDSCHETNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVL------------------------SFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICS 2730
            P  +CP KCTCS+  V+C   GL ++P  I  + + L +  NN+ ++                                                P       P L +LDLS NQI+ +    F   + ++ + L++N + CI +  F+ L++LEIL L+ N IS I+  SF +   ++ L L+ N  +CDC L W+S+W R   T+    +   C +P+ L+   +  +  K ++C G  S   + ++   + S  + CS  N                   VDC    L EIP  +P+   E+ L++N ++S+   +F     LK + +S N I+++  +    LKSL +LVL                        + NK+ C+  + F++L NL +L L  N + TI    F  L+++  + L QNPF CDC++KWL  +  D  ++   + C+SP ++  K I   K   F CS
Sbjct:   30 PAAACPTKCTCSAASVDCHGLGLRAVPRGIPRNAERLDLDRNNITRITKMDFAGLKNLRVLHLEDNQVSIIERGAFQDLKQLERLRLNKNKLQVLPELLFQSTPKLTRLDLSENQIQGIPRKAFRGVTGVKNLQLDNNHISCIEDGAFRALRDLEILTLNNNNISRILVTSFNHMPKIRTLRLHSNHLYCDCHLAWLSDWLRQRRTI---GQFTLCMAPVHLRGFSVADVQKKEYVCPGPHSEAPACNA--NSLSCPSACSCSN-----------------NIVDCRGKGLTEIPANLPEGIVEIRLEQNSIKSIPAGAFTQYKKLKRIDISKNQISDIAPDAFQGLKSLTSLVLYGNKITEIPKGLFDGLVSLQLLLLNANKINCLRVNTFQDLQNLNLLSLYDNKLQTISKGLFVPLQSIQTLHLAQNPFVCDCHLKWLADYLQDNPIETSGARCSSPRRLANKRISQIKSKKFRCS 486          

HSP 3 Score: 56.225 bits (134), Expect = 3.437e-6
Identity = 39/115 (33.91%), Postives = 59/115 (51.30%), Query Frame = 1
Query: 2905 CRNGGICKNIDNYGNYECVCPVGFRGPKCTINV-DDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCND-INPCQ----NSGQCQLLPNNDYKCVCSKGWEGKQCER 3231
            CR+G +C++++   +  C C  G+ GP C     D C+G+ C +G TC    +SY CKC  G+ G  CD K    ND  + C     + GQC +    +  C+C  G+ G  CE+
Sbjct: 1338 CRHG-LCRSVEK-DSVVCECHPGWTGPLCDQEARDPCLGHSCRHG-TCMATGDSYVCKCAEGYGGALCDQK----NDSASACSAFKCHHGQCHISDRGEPYCLCQPGFSGHHCEQ 1445          
BLAST of slit1 vs. UniProt/SwissProt
Match: sp|O88280|SLIT3_RAT (Slit homolog 3 protein OS=Rattus norvegicus OX=10116 GN=Slit3 PE=2 SV=1)

HSP 1 Score: 726.472 bits (1874), Expect = 0.000e+0
Identity = 463/1378 (33.60%), Postives = 706/1378 (51.23%), Query Frame = 1
Query:  148 SEICPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQLQ------------------------CINDNIFNKLGMLKYLLLEGNKIFWI-SEAISKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCASEDKANLENMCPSDKKVCSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQIYNFQC--NKEFESKYETHDEECVPKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLPPIYSIHEAPNLMKLDLS------------------------FNQIETLEPDVFTRSSKLREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQFHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCDGNASVYHSVDSCHETNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVLSFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGN-YECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYC-NDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNSIYF-QKNSYIEISPPSRGFLMPRGNISLNFMTNSSSGLILYQGSEQTYLVVNLYQGHLRISYNLGDKVVGPEG--YSMGMVNDSRLHALHIEIVGENLTMYLNGRVHCTLKSKYLNSNRYMALDTGIFIGGAPHKILQSAIMKFHIETDSEFIGCMESLLINRKKIDFSKYLPKSINVHPGC 4113
            +  CP  C C   SV+C       VP  +     RL+L  N I  I K DF  L NL+VL L +N+++ IE GAF +L  L +LRLN+N +Q LP+ LF    KL +LDL +NQ+Q                        CI D  F  L  L+ L L  N I  I   + + +  ++ LR+H N L C+CHLAWL+ +L+Q+  I        LC  P  L    ++ +   E+ C           C ++   C   C+CS++ IV C GK L+ IP +LP  +VE+ L+ N+I  +  G   +YK LK+I +++N + DI P AF GL  LTS+ L  N +  I K  F GLVSL  L L  N I CL  +T +  + L+LL L +NKL ++ K  F P  +++ L+L  NP  CDC + W+ DY++D    T  A C  PR L    +  ++   F+C  ++++ +++ +   EC   + CP KC C   +V+CSN  L  +P  +   T  L ++ N++  L       + PNL K++LS                         NQ+ET+   +F   S L+ + L SN + C+NN+TF  L  + +L L +N I+ I   +F   ++L  + L  N F+C+C + W+  W R    V GN   P CQ P  LK+ PI  +  + F C+GN       +SC  +      C+                    T V CS+  L  +P  +PKD TELYL+ NHL ++ +        L  + LS N I+ L  +  + +  L TL+LS+N+L+CI   AF  L +L+VL L  ND+S++P  +FNDL +L+++ALG NP HCDC+++WL+++    + + GI+ C+SPE M  + +  +    F C     D  I AKC++C+  PCK+NG CS      Y+C CPY + GK+C   IN C   PC++GG C   +++ + + C CP+GF G +C IN DDC  N C N ATC D  N+Y+C C   + G+ CD    YC  ++N CQ+  +C  L +  ++C C  G+ GK CE + DDC  + C+H +QCVD  N Y C+C  G+ G FCE P   P VL                       +  S C  +EC N   CI       Q+  C+C PG++G  C+ L ++ F  K+SY+E++      + P+ NISL   T+  +G++LY+G +   L + LYQGH+R+ Y   D +  P    YS+  VND + H++ + ++ + L + ++     +L    L     + +++ +++GG P     SA+ +        F GC+  + IN +  DF    P+S+ V PGC
Sbjct:   31 AAACPTKCTCSAASVDCHGLGLRAVPRGIPRNAERLDLDRNNITRITKMDFTGLKNLRVLHLEDNQVSVIERGAFQDLKQLERLRLNKNKLQVLPELLFQSTPKLTRLDLSENQIQGIPRKAFRGVTGVKNLQLDNNHISCIEDGAFRALRDLEILTLNNNNISRILVTSFNHMPKIRTLRLHSNHLYCDCHLAWLSDWLRQRRTI----GQFTLCMAPVHLRGFSVADVQKKEYVCPGPHSE--APACNANSLSCPSACSCSNN-IVDCRGKGLTEIPANLPEGIVEIRLEQNSIKSIPAGAFIQYKKLKRIDISKNQISDIAPDAFQGLKSLTSLVLYGNKITEIPKGLFDGLVSLQLLLLNANKINCLRVNTFQDLQNLNLLSLYDNKLQTISKGLFAPLQSIQTLHLAQNPFVCDCHLKWLADYLQDNPIETSGARCSSPRRLANKRISQIKSKKFRCSGSEDYRNRFSS---ECFMDLVCPEKCRCEGTIVDCSNQKLSRIPSHLPEYTTDLRLNDNDIAVLEATGIFKKLPNLRKINLSNNRIKEVREGAFDGAAGVQELMLTGNQLETMHGRMFRGLSGLKTLMLRSNLISCVNNDTFAGLSSVRLLSLYDNRITTISPGAFTTLVSLSTINLLSNPFNCNCHMAWLGRWLRKRRIVSGN---PRCQKPFFLKEIPIQDVAIQDFTCEGNEE-----NSCQLSPRCPEQCTC-----------------VETVVRCSNRGLHTLPKGMPKDVTELYLEGNHLTAVPK-ELSTFRQLTLIDLSNNSISMLTNHTFSNMSHLSTLILSYNRLRCIPVHAFNGLRSLRVLTLHGNDISSVPEGSFNDLTSLSHLALGINPLHCDCSLRWLSEWIKAGYKEPGIARCSSPESMADRLLLTTPTHRFQCKGPV-DINIVAKCNACLSSPCKNNGTCSQDPVEQYRCTCPYSYKGKDCTVPINTCVQNPCQHGGTCHLSESHRDGFSCSCPLGFEGQRCEINPDDCEDNDCENSATCVDGINNYACVCPPNYTGELCDEVIDYCVPEMNLCQHEAKCISL-DKGFRCECVPGYSGKLCETDNDDCVAHKCRHGAQCVDAVNGYTCICPQGFSGLFCEHPP--PMVL-----------------------LQTSPCDQYECQNGAQCI----VVQQEPTCRCPPGFAGPRCEKLITVNFVGKDSYVELASAK---VRPQANISLQVATDKDNGILLYKG-DNDPLALELYQGHVRLVY---DSLSSPPTTVYSVETVNDGQFHSVELVMLNQTLNLVVDKGAPKSLGK--LQKQPAVGINSPLYLGGIPTSTGLSALRQGADRPLGGFHGCIHEVRINNELQDFKALPPQSLGVSPGC 1332          

HSP 2 Score: 185.652 bits (470), Expect = 1.060e-45
Identity = 128/479 (26.72%), Postives = 213/479 (44.47%), Query Frame = 1
Query: 1507 PKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLP-----------------------------------------------PIYSIHEAPNLMKLDLSFNQIETLEPDVFTRSSKLREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQFHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCDGNASVYHSVDSCHETNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVL------------------------SFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICS 2730
            P  +CP KCTCS+  V+C   GL ++P  I  + + L +  NN+ ++                                                P       P L +LDLS NQI+ +    F   + ++ + L++N + CI +  F+ L++LEIL L+ N IS I+  SF +   ++ L L+ N  +CDC L W+S+W R   T+    +   C +P+ L+   +  +  K ++C G  S   + ++   + S  + CS  N                   VDC    L EIP  +P+   E+ L++N ++S+   +F     LK + +S N I+++  +    LKSL +LVL                        + NK+ C+  + F++L NL +L L  N + TI    F  L+++  + L QNPF CDC++KWL  +  D  ++   + C+SP ++  K I   K   F CS
Sbjct:   30 PAAACPTKCTCSAASVDCHGLGLRAVPRGIPRNAERLDLDRNNITRITKMDFTGLKNLRVLHLEDNQVSVIERGAFQDLKQLERLRLNKNKLQVLPELLFQSTPKLTRLDLSENQIQGIPRKAFRGVTGVKNLQLDNNHISCIEDGAFRALRDLEILTLNNNNISRILVTSFNHMPKIRTLRLHSNHLYCDCHLAWLSDWLRQRRTI---GQFTLCMAPVHLRGFSVADVQKKEYVCPGPHSEAPACNA--NSLSCPSACSCSN-----------------NIVDCRGKGLTEIPANLPEGIVEIRLEQNSIKSIPAGAFIQYKKLKRIDISKNQISDIAPDAFQGLKSLTSLVLYGNKITEIPKGLFDGLVSLQLLLLNANKINCLRVNTFQDLQNLNLLSLYDNKLQTISKGLFAPLQSIQTLHLAQNPFVCDCHLKWLADYLQDNPIETSGARCSSPRRLANKRISQIKSKKFRCS 486          

HSP 3 Score: 60.077 bits (144), Expect = 2.373e-7
Identity = 41/115 (35.65%), Postives = 59/115 (51.30%), Query Frame = 1
Query: 2905 CRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDD-CVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCND-INPCQ----NSGQCQLLPNNDYKCVCSKGWEGKQCER 3231
            CR+G +C++++   +  C C  G+ GP C     D C+G+ C +G TC    NSY CKC  G+ G  CD K    ND  N C     + GQC +    +  C+C  G+ G  CE+
Sbjct: 1338 CRHG-LCRSVEK-DSVVCECHPGWTGPLCDQEAQDPCLGHSCSHG-TCVATGNSYVCKCAEGYEGPLCDQK----NDSANACSAFKCHHGQCHISDRGEPYCLCQPGFSGNHCEQ 1445          
BLAST of slit1 vs. UniProt/SwissProt
Match: sp|P24014|SLIT_DROME (Protein slit OS=Drosophila melanogaster OX=7227 GN=sli PE=1 SV=2)

HSP 1 Score: 726.087 bits (1873), Expect = 0.000e+0
Identity = 484/1534 (31.55%), Postives = 751/1534 (48.96%), Query Frame = 1
Query:  157 CPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQLQCINDNIFNKLGMLKYLLLEGNKIFWISEAISK-------------------------LKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCAS-EDKANLENMCPSDKKVCSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQIYNFQCN-KEFESKYETHDEECVPKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLPPIYSIHEAPNLMKLDLSFNQIETLEPDVFTRSSKLREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQFHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCDGNASVYHSVDSCHETNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVLSFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNL-CVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCN-DINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNSIYF-QKNSYIEISPPSRGFLMPRGNISLNFMTNSSSGLILYQGSEQTYLVVNLYQGHLRISYNLGDKVVGPEGYSMGMVNDSRLHALHIEIVGENLTMYLNGRVHCTLKSKYLN--SNRYMALDTGIFIGGAPHKILQSAIMKFHIETDSEFIGCMESLLINRKKIDFSKYLPKSINVHPGCGKLISTVVASDLKIPSIMXXXXXXXXXXXXXXXNNVCKRPGICKNQGVCEVLPGSKKK--FRCICKNGFVGRKCKKRKS-------MCHGTFTESHIVDPDSQGTCKSIERFQYRICTGTCSTSRMQSDVIKNFYHKPIDPSSSRCCQPTKYSTHEIQFKCENGRVYRRAANLPKNCKCVANC 4635
            CP  C C   +V+C HR   +VP  +     RL L GN + VI ++DF +L  L++LQL++N+I +IE  +F +LV+L +LRLN N ++ +P++  +    L +LD+ +N +  +   +F     L+ L L+ N+I  + E   K                         L  L+ LR+  N   C+CHL+WL+ FL+  + +    A    C +P +L    ++ L   EF+C+   + A +E  C ++   C   C C+ DGIV C  K L+++P  LP +  EL L+ N IT L     S ++ L++I L+ N +  I   A  GL QLT++ L  N +K +    F GL SL  L L  N+I C+ KD       L LL L +N + SL   TF    +++ ++L  NP  CDC + W+ DY+      T  A C  P+ +    + +L+   F+C+  E   K      EC     CP  C C    V+C+  GL  +P  I   T  L+++ N L ++         P+L+KL+L  NQ+  +EP+ F  +S ++E+ L  N++K I+N+ F  L +L+ L L +N ISC++  SF++  +L  L L  N F+C+C L W +EW R      G  R   C +P  ++D  I  L    F C    S     + C         C+                    T V CS  QLKEIP  IP +T+ELYL+ N ++ ++    ++L +L  L LS N IT L+      L  L TL++S+NKLQC+ + A   L+NL+VL L  N +S +P  +F DLK+L +IALG NP +CDC +KW + +    +++ GI+ CA PE+MK K I  +  + F+C     +  I AKC++C ++PC++  +C  +    YQC C   +HGK+C+  I+AC+G PCRN   C  ++  G + C C  G+ G +C  N+DDC+G + C N ATC D   SY C+CQ GF G++CD K  +C+ + NPC N  +C +     Y C C  G+ G  C  N DDC+ + C++   CVD  NDY+C C   Y GK+CE                  N   M Y          S C  HEC  +G+C   +  +     C+C PGY+G  C+YL SI F   NS++E+ P       P  N+++ F +   +G+++Y G +  +L V L+ G +R+SY++G+  V    YS  MV D + HA+ +  + +N T+    RV   L    +N  SN Y+ L T +F+GG P    Q A   + I   + F GCM+ + IN K +DF     +   + PGC  L       +      M     I  +       N C+R   C        +P S  +  ++C CK+G  GR C + +            T  +  + +  ++  C+S +  +Y  C G C                      ++CC        +++  C N R Y +  ++ + C C   C
Sbjct:   73 CPRVCSCTGLNVDCSHRGLTSVPRKISADVERLELQGNNLTVIYETDFQRLTKLRMLQLTDNQIHTIERNSFQDLVSLERLRLNNNRLKAIPENFVTSSASLLRLDISNNVITTVGRRVFKGAQSLRSLQLDNNQITCLDEHAFKGLVELEILTLNNNNLTSLPHNIFGGLGRLRALRLSDNPFACDCHLSWLSRFLRSATRL----APYTRCQSPSQLKGQNVADLHDQEFKCSGLTEHAPME--CGAENS-CPHPCRCA-DGIVDCREKSLTSVPVTLPDDTTELRLEQNFITELPPKSFSSFRRLRRIDLSNNNISRIAHDALSGLKQLTTLVLYGNKIKDLPSGVFKGLGSLQLLLLNANEISCIRKDAFRDLHSLSLLSLYDNNIQSLANGTFDAMKSIKTVHLAKNPFICDCNLRWLADYLHKNPIETSGARCESPKRMHRRRIESLREEKFKCSWDELRMKLSG---ECRMDSDCPAMCHCEGTTVDCTGRGLKEIPRDIPLHTTELLLNDNELGRISSDGLFGRLPHLVKLELKRNQLTGIEPNAFEGASHIQELQLGENKIKEISNKMFLGLHQLKTLNLYDNQISCVMPGSFEHLNSLTSLNLASNPFNCNCHLAWFAEWLRKKSLNGGAAR---CGAPSKVRDVQIKDLPHSEFKCSSENS-----EGCLGDGYCPPSCTC-----------------TGTVVRCSRNQLKEIPRGIPAETSELYLESNEIEQIHYERIRHLRSLTRLDLSNNQITILSNYTFANLTKLSTLIISYNKLQCLQRHALSGLNNLRVLSLHGNRISMLPEGSFEDLKSLTHIALGSNPLYCDCGLKWFSDWIKLDYVEPGIARCAEPEQMKDKLILSTPSSSFVCRGRVRND-ILAKCNACFEQPCQNQAQCVALPQREYQCLCQPGYHGKHCEFMIDACYGNPCRNNATCTVLEE-GRFSCQCAPGYTGARCETNIDDCLGEIKCQNNATCIDGVESYKCECQPGFSGEFCDTKIQFCSPEFNPCANGAKC-MDHFTHYSCDCQAGFHGTNCTDNIDDCQNHMCQNGGTCVDGINDYQCRCPDDYTGKYCE----------------GHNMISMMYPQT-------SPCQNHEC-KHGVCFQPNA-QGSDYLCRCHPGYTGKWCEYLTSISFVHNNSFVELEPLR---TRPEANVTIVFSSAEQNGILMYDG-QDAHLAVELFNGRIRVSYDVGNHPVSTM-YSFEMVADGKYHAVELLAIKKNFTL----RVDRGLARSIINEGSNDYLKLTTPMFLGGLPVDPAQQAYKNWQIRNLTSFKGCMKEVWINHKLVDFGN-AQRQQKITPGCALLEGEQQEEEDDEQDFMDETPHIKEEPVDPCLENKCRRGSRC--------VPNSNARDGYQCKCKHGQRGRYCDQGEGSTEPPTVTAASTCRKEQVREYYTENDCRSRQPLKYAKCVGGC---------------------GNQCCAAKIVRRRKVRMVCSNNRKYIKNLDIVRKCGCTKKC 1503          

HSP 2 Score: 175.252 bits (443), Expect = 1.342e-42
Identity = 135/492 (27.44%), Postives = 218/492 (44.31%), Query Frame = 1
Query: 1504 VPKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLK-----------------------------------------------KLPPIYSIHEAPNLMKLDLSFNQIETLEPDVFTRSSKLREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQFHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCDGNASVYHSVDSCHETNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVLSFNKLQ------------------------CIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKC 2766
            + +  CP  C+C+   V+CS+ GL S+P  I    + L +  NNL                                                K  P   +  + +L++LD+S N I T+   VF  +  LR + L++N++ C++   FK L ELEIL L+ N ++ +  + F     L+ L L+ N F CDC L W+S + R+   +    R   CQSP  LK   +  L D+ F C G     H+   C   NS    C   +                   VDC +  L  +P  +P DTTEL L++N +  L   SF +   L+ + LS N I+ +  + L+ LK L TLVL  NK++                        CI +DAF++LH+L +L L  N++ ++    F+ +K++  + L +NPF CDCN++WL  +     ++   + C SP++M  + I   +   F CS +     ++ +C
Sbjct:   68 ITEARCPRVCSCTGLNVDCSHRGLTSVPRKISADVERLELQGNNLTVIYETDFQRLTKLRMLQLTDNQIHTIERNSFQDLVSLERLRLNNNRLKAIPENFVTSSASLLRLDISNNVITTVGRRVFKGAQSLRSLQLDNNQITCLDEHAFKGLVELEILTLNNNNLTSLPHNIFGGLGRLRALRLSDNPFACDCHLSWLSRFLRSATRLAPYTR---CQSPSQLKGQNVADLHDQEFKCSG--LTEHAPMECGAENSCPHPCRCAD-----------------GIVDCREKSLTSVPVTLPDDTTELRLEQNFITELPPKSFSSFRRLRRIDLSNNNISRIAHDALSGLKQLTTLVLYGNKIKDLPSGVFKGLGSLQLLLLNANEISCIRKDAFRDLHSLSLLSLYDNNIQSLANGTFDAMKSIKTVHLAKNPFICDCNLRWLADYLHKNPIETSGARCESPKRMHRRRIESLREEKFKCSWDELRMKLSGEC 537          
BLAST of slit1 vs. UniProt/SwissProt
Match: sp|O75094|SLIT3_HUMAN (Slit homolog 3 protein OS=Homo sapiens OX=9606 GN=SLIT3 PE=2 SV=3)

HSP 1 Score: 723.776 bits (1867), Expect = 0.000e+0
Identity = 464/1375 (33.75%), Postives = 701/1375 (50.98%), Query Frame = 1
Query:  157 CPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQLQ------------------------CINDNIFNKLGMLKYLLLEGNKIFWI-SEAISKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCASEDKANLENMCPSDKKVCSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQIYNFQC--NKEFESKYETHDEECVPKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLPPIYSIHEAPNLMKLDLS------------------------FNQIETLEPDVFTRSSKLREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQFHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCDGNASVYHSVDSCHETNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVLSFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGN-YECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYC-NDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNSIYF-QKNSYIEISPPSRGFLMPRGNISLNFMTNSSSGLILYQGSEQTYLVVNLYQGHLRISYNLGDKVVGPEG--YSMGMVNDSRLHALHIEIVGENLTMYLNGRVHCTLKSKYLNSNRYMALDTGIFIGGAPHKILQSAIMKFHIETDSEFIGCMESLLINRKKIDFSKYLPKSINVHPGC 4113
            CP  C C   SV+C       VP  +     RL+L  N I  I K DF  L NL+VL L +N+++ IE GAF +L  L +LRLN+N +Q LP+ LF    KL +LDL +NQ+Q                        CI D  F  L  L+ L L  N I  I   + + +  ++ LR+H N L C+CHLAWL+ +L+Q+  +        LC  P  L    ++ +   E+ C +         C ++   C   C CS++ IV C GK L  IP +LP  +VE+ L+ N+I  +  G  ++YK LK+I +++N + DI P AF GL  LTS+ L  N +  I K  F GLVSL  L L  N I CL  +T +  + L+LL L +NKL ++ K  F P  +++ L+L  NP  CDC + W+ DY++D    T  A C  PR L    +  ++   F+C  ++++ S++ +   EC   + CP KC C   +V+CSN  L+ +P  +      L ++ N +  L       + PNL K++LS                         NQ+ET+   VF   S L+ + L SN + C++N+TF  L  + +L L +N I+ I   +F   ++L  + L  N F+C+C L W+ +W R    V GN   P CQ P  LK+ PI  +  + F CDGN        SC  +      C+                    T V CS+  L+ +P  +PKD TELYL+ NHL ++       L +L  + LS N I+ L     + +  L TL+LS+N+L+CI   AF  L +L+VL L  ND+S++P  +FNDL +L+++ALG NP HCDC+++WL+++    + + GI+ C+SPE M  + +  +    F C     D  I AKC++C+  PCK+NG C+      Y+C CPY + GK+C   IN C   PC++GG C   D++ + + C CP+GF G +C IN DDC  N C N ATC D  N+Y C C   + G+ CD    +C  ++N CQ+  +C  L +  + C C  G+ GK CE + DDC  + C+H +QCVD  N Y C C  G+ G FCE P   P VL                       +  S C  +EC N   CI       Q+  C+C PG++G  C+ L ++ F  K+SY+E+   +   + P+ NISL   T+  +G++LY+G +   L + LYQGH+R+ Y   D +  P    YS+  VND + H++ +  + + L + ++     +L    L     + +++ +++GG P     SA+ +        F GC+  + IN +  DF    P+S+ V PGC
Sbjct:   34 CPTKCTCSAASVDCHGLGLRAVPRGIPRNAERLDLDRNNITRITKMDFAGLKNLRVLHLEDNQVSVIERGAFQDLKQLERLRLNKNKLQVLPELLFQSTPKLTRLDLSENQIQGIPRKAFRGITDVKNLQLDNNHISCIEDGAFRALRDLEILTLNNNNISRILVTSFNHMPKIRTLRLHSNHLYCDCHLAWLSDWLRQRRTV----GQFTLCMAPVHLRGFNVADVQKKEYVCPAPHSE--PPSCNANSISCPSPCTCSNN-IVDCRGKGLMEIPANLPEGIVEIRLEQNSIKAIPAGAFTQYKKLKRIDISKNQISDIAPDAFQGLKSLTSLVLYGNKITEIVKGLFDGLVSLQLLLLNANKINCLRVNTFQDLQNLNLLSLYDNKLQTISKGLFAPLQSIQTLHLAQNPFVCDCHLKWLADYLQDNPIETSGARCSSPRRLANKRISQIKSKKFRCSGSEDYRSRFSS---ECFMDLVCPEKCRCEGTIVDCSNQKLVRIPSHLPEYVTDLRLNDNEVSVLEATGIFKKLPNLRKINLSNNKIKEVREGAFDGAASVQELMLTGNQLETVHGRVFRGLSGLKTLMLRSNLIGCVSNDTFAGLSSVRLLSLYDNRITTITPGAFTTLVSLSTINLLSNPFNCNCHLAWLGKWLRKRRIVSGN---PRCQKPFFLKEIPIQDVAIQDFTCDGNEE-----SSCQLSPRCPEQCTC-----------------METVVRCSNKGLRALPRGMPKDVTELYLEGNHLTAVPR-ELSALRHLTLIDLSNNSISMLTNYTFSNMSHLSTLILSYNRLRCIPVHAFNGLRSLRVLTLHGNDISSVPEGSFNDLTSLSHLALGTNPLHCDCSLRWLSEWVKAGYKEPGIARCSSPEPMADRLLLTTPTHRFQCKGPV-DINIVAKCNACLSSPCKNNGTCTQDPVELYRCACPYSYKGKDCTVPINTCIQNPCQHGGTCHLSDSHKDGFSCSCPLGFEGQRCEINPDDCEDNDCENNATCVDGINNYVCICPPNYTGELCDEVIDHCVPELNLCQHEAKCIPL-DKGFSCECVPGYSGKLCETDNDDCVAHKCRHGAQCVDTINGYTCTCPQGFSGPFCEHPP--PMVL-----------------------LQTSPCDQYECQNGAQCI----VVQQEPTCRCPPGFAGPRCEKLITVNFVGKDSYVEL---ASAKVRPQANISLQVATDKDNGILLYKG-DNDPLALELYQGHVRLVY---DSLSSPPTTVYSVETVNDGQFHSVELVTLNQTLNLVVDKGTPKSLGK--LQKQPAVGINSPLYLGGIPTSTGLSALRQGTDRPLGGFHGCIHEVRINNELQDFKALPPQSLGVSPGC 1332          

HSP 2 Score: 195.282 bits (495), Expect = 1.141e-48
Identity = 140/469 (29.85%), Postives = 229/469 (48.83%), Query Frame = 1
Query:  154 ICPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSD-FLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQLQCINDNIFNKLGMLKYLLLEGNKIFWISE-AISKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCASEDKANLENMCPSDKKVCSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQIYNFQCNKEFESKYETHDEECVPKVSCPLKCTCSSDVV 1554
            +CP  C+CE   V+C ++    +P  L E  T L L  N ++V+  +  F KL NL+ + LSNN+I  +  GAFD   ++ +L L  N ++ +   +F  L+ L+ L LR N + C++++ F  L  ++ L L  N+I  I+  A + L  L  + +  N  NCNCHLAWL  +L+++  +    + +  C  PF L  IPI  ++  +F C      N E+ C    + C ++C C  + +V+C  K L  +P+ +P +V EL L+ N++T + +  LS  + L  I L+ N +  +    F  ++ L+++ L+ N L+ I  +AF GL SL  L L  NDI  +P+ +              N LTSL            +L L  NPL+CDC + W+ +++K       +A C  P  +    L     + FQC    +         C+    C    TC+ D V
Sbjct:  504 VCPEKCRCEGTIVDCSNQKLVRIPSHLPEYVTDLRLNDNEVSVLEATGIFKKLPNLRKINLSNNKIKEVREGAFDGAASVQELMLTGNQLETVHGRVFRGLSGLKTLMLRSNLIGCVSNDTFAGLSSVRLLSLYDNRITTITPGAFTTLVSLSTINLLSNPFNCNCHLAWLGKWLRKRRIV----SGNPRCQKPFFLKEIPIQDVAIQDFTC----DGNEESSCQLSPR-CPEQCTC-METVVRCSNKGLRALPRGMPKDVTELYLEGNHLTAVPR-ELSALRHLTLIDLSNNSISMLTNYTFSNMSHLSTLILSYNRLRCIPVHAFNGLRSLRVLTLHGNDISSVPEGSF-------------NDLTSLS-----------HLALGTNPLHCDCSLRWLSEWVKAGYKEPGIARCSSPEPMADRLLLTTPTHRFQCKGPVDINIVAKCNACL-SSPCKNNGTCTQDPV 936          

HSP 3 Score: 182.956 bits (463), Expect = 6.883e-45
Identity = 128/479 (26.72%), Postives = 213/479 (44.47%), Query Frame = 1
Query: 1507 PKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLP-----------------------------------------------PIYSIHEAPNLMKLDLSFNQIETLEPDVFTRSSKLREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQFHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCDGNASVYHSVDSCHETNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVL------------------------SFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICS 2730
            P V+CP KCTCS+  V+C   GL ++P  I  + + L +  NN+ ++                                                P       P L +LDLS NQI+ +    F   + ++ + L++N + CI +  F+ L++LEIL L+ N IS I+  SF +   ++ L L+ N  +CDC L W+S+W R   TV    +   C +P+ L+   +  +  K ++C    S   S ++   + S  + C+  N                   VDC    L EIP  +P+   E+ L++N ++++   +F     LK + +S N I+++  +    LKSL +LVL                        + NK+ C+  + F++L NL +L L  N + TI    F  L+++  + L QNPF CDC++KWL  +  D  ++   + C+SP ++  K I   K   F CS
Sbjct:   30 PAVACPTKCTCSAASVDCHGLGLRAVPRGIPRNAERLDLDRNNITRITKMDFAGLKNLRVLHLEDNQVSVIERGAFQDLKQLERLRLNKNKLQVLPELLFQSTPKLTRLDLSENQIQGIPRKAFRGITDVKNLQLDNNHISCIEDGAFRALRDLEILTLNNNNISRILVTSFNHMPKIRTLRLHSNHLYCDCHLAWLSDWLRQRRTV---GQFTLCMAPVHLRGFNVADVQKKEYVCPAPHSEPPSCNA--NSISCPSPCTCSN-----------------NIVDCRGKGLMEIPANLPEGIVEIRLEQNSIKAIPAGAFTQYKKLKRIDISKNQISDIAPDAFQGLKSLTSLVLYGNKITEIVKGLFDGLVSLQLLLLNANKINCLRVNTFQDLQNLNLLSLYDNKLQTISKGLFAPLQSIQTLHLAQNPFVCDCHLKWLADYLQDNPIETSGARCSSPRRLANKRISQIKSKKFRCS 486          
BLAST of slit1 vs. UniProt/SwissProt
Match: sp|O94813|SLIT2_HUMAN (Slit homolog 2 protein OS=Homo sapiens OX=9606 GN=SLIT2 PE=1 SV=1)

HSP 1 Score: 702.205 bits (1811), Expect = 0.000e+0
Identity = 486/1491 (32.60%), Postives = 731/1491 (49.03%), Query Frame = 1
Query:  151 EICPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQLQ------------------------CINDNIFNKLGMLKYLLLEGNKIFWISEA-ISKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCASEDKANLENMCPSDKKV-CSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQIYNFQCN----------KEFESKYETHDEECVPKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLPPIYSIHEAPNLMKLDLSFNQIETLEPDVFTRSSKLREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQ------------------------FHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCD-GNASVYHSVDSCHETNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVLSFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGIC--KNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYC-NDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNSIYF-QKNSYIEISPPSRGFLMPRGNISLNFMTNSSSGLILYQGSEQTYLVVNLYQGHLRISYNLGDKVVGPEGYSMGMVNDSRLHALHIEIVGENLTMYLNGRVHCTLKSKYLNSNRYMALDTGIFIGGAPHKILQSAIMKFHIETDSEFIGCMESLLINRKKIDFSKYLPKSINVHPGCGKLISTVVASDLKIPSIMXXXXXXXXXXXXXXXNNVCKRPGICKNQGVCEVLPGSKKKFRCICKNGFVGRKCKKRKSMCHGTFTESHIVDPDSQGTCKSIERFQY 4428
            + CP  C C   +V+C      +VP  +   T RL+L GN I  I K+DF  L +L+VLQL  N+I++IE GAF +L  L +LRLNRN +Q  P+ LF    KL +LDL +NQ+Q                        CI D  F  L  L+ L L  N I  +S A  + +  L+  R+H N L C+CHLAWL+ +L+Q+  +   G ++  C  P  L    ++ +   EF C+         M PS   + C   C CS++ IV C GK L+ IP +LP  + E+ L+ N I ++  G  S YK L++I L+ N + ++ P AF GL  L S+ L  N +  + K+ F GL SL  L L  N I CL  D  +    L+LL L +NKL ++ K TF P   ++ ++L  NP  CDC + W+ DY+      T  A C  PR L    +  ++   F+C+          +++ SK      +C   ++CP KC C    V+CSN  L  +PE I   T  L +++N    L       + P L K++ S N+I  +E   F  +S + EI L SNRL+ + ++ FK L+ L+ L L  N I+C+  DSF    +++ L L  NQ                        F+C+C L W+ EW R    V GN   P CQ P  LK+ PI  +  + F CD GN       +SC   +   T C+                    T V CS+  LK +P  IP+D TELYLD N   +L      N  +L  + LS N I+ L+    + +  L TL+LS+N+L+CI    F  L +L++L L  ND+S +P  AFNDL  L+++A+G NP +CDCN++WL+ +    + + GI+ CA P +M  K +  +    F C     D  I AKC+ C+  PCK++G C+      Y+C CPY F G++CD  I+AC   PC++GG C  K  +  G + C+C  GF G  C +NVDDC  N C N +TC D  N+Y+C C   + G+ C+ K  +C  D+NPCQ+  +C L P   +KC C+ G+ G+ C+ + DDC+ N CK+ + C D  N Y C+C  GY G FCE     P VL                          S C   +C N   CI     +  +  C+C PGY G  C+ L S+ F  K SY++I P ++  + P+ NI+L   T+  SG++LY+G ++ ++ V LY+G +R SY+ G        YS+  +ND   H + +  + ++L++ ++G     + +  L+    +  D+ +++GG P K   +++ +   +  + F GC+ +L IN +  DF K +P    + PGC      V A                                     G C+  P S+  F C C+ G++G  C +R +       +  + +    GTC  I  F Y
Sbjct:   26 QACPAQCSCSGSTVDCHGLALRSVPRNIPRNTERLDLNGNNITRITKTDFAGLRHLRVLQLMENKISTIERGAFQDLKELERLRLNRNHLQLFPELLFLGTAKLYRLDLSENQIQAIPRKAFRGAVDIKNLQLDYNQISCIEDGAFRALRDLEVLTLNNNNITRLSVASFNHMPKLRTFRLHSNNLYCDCHLAWLSDWLRQRPRV---GLYTQ-CMGPSHLRGHNVAEVQKREFVCSGHQSF----MAPSCSVLHCPAACTCSNN-IVDCRGKGLTEIPTNLPETITEIRLEQNTIKVIPPGAFSPYKKLRRIDLSNNQISELAPDAFQGLRSLNSLVLYGNKITELPKSLFEGLFSLQLLLLNANKINCLRVDAFQDLHNLNLLSLYDNKLQTIAKGTFSPLRAIQTMHLAQNPFICDCHLKWLADYLHTNPIETSGARCTSPRRLANKRIGQIKSKKFRCSAKEQYFIPGTEDYRSKLSG---DCFADLACPEKCRCEGTTVDCSNQKLNKIPEHIPQYTAELRLNNNEFTVLEATGIFKKLPQLRKINFSNNKITDIEEGAFEGASGVNEILLTSNRLENVQHKMFKGLESLKTLMLRSNRITCVGNDSFIGLSSVRLLSLYDNQITTVAPGAFDTLHSLSTLNLLANPFNCNCYLAWLGEWLRKKRIVTGN---PRCQKPYFLKEIPIQDVAIQDFTCDDGNDD-----NSCSPLSRCPTECTC-----------------LDTVVRCSNKGLKVLPKGIPRDVTELYLDGNQF-TLVPKELSNYKHLTLIDLSNNRISTLSNQSFSNMTQLLTLILSYNRLRCIPPRTFDGLKSLRLLSLHGNDISVVPEGAFNDLSALSHLAIGANPLYCDCNMQWLSDWVKSEYKEPGIARCAGPGEMADKLLLTTPSKKFTCQGPV-DVNILAKCNPCLSNPCKNDGTCNSDPVDFYRCTCPYGFKGQDCDVPIHACISNPCKHGGTCHLKEGEEDG-FWCICADGFEGENCEVNVDDCEDNDCENNSTCVDGINNYTCLCPPEYTGELCEEKLDFCAQDLNPCQHDSKCILTPKG-FKCDCTPGYVGEHCDIDFDDCQDNKCKNGAHCTDAVNGYTCICPEGYSGLFCEFSP--PMVLP-----------------------RTSPCDNFDCQNGAQCI----VRINEPICQCLPGYQGEKCEKLVSVNFINKESYLQI-PSAK--VRPQTNITLQIATDEDSGILLYKG-DKDHIAVELYRGRVRASYDTGSHPASAI-YSVETINDGNFHIVELLALDQSLSLSVDGGNPKIITN--LSKQSTLNFDSPLYVGGMPGKSNVASLRQAPGQNGTSFHGCIRNLYINSELQDFQK-VPMQTGILPGCEPCHKKVCA------------------------------------HGTCQ--PSSQAGFTCECQEGWMGPLCDQRTN-------DPCLGNKCVHGTCLPINAFSY 1393          

HSP 2 Score: 58.9214 bits (141), Expect = 4.987e-7
Identity = 43/111 (38.74%), Postives = 56/111 (50.45%), Query Frame = 1
Query: 2917 GICKNIDNYGNYECVCPVGFRGPKCTINVDD-CVGNLCVNGATCNDLNN-SYSCKCQSGFRGKYCDIKFLYCNDINPCQ----NSGQCQLLPNNDYKCVCSKGWEGKQCER 3231
            G C+     G + C C  G+ GP C    +D C+GN CV+G TC  +N  SYSCKC  G  G  CD +    +  NPCQ      G+C+L       C CS G+ G  C+R
Sbjct: 1344 GTCQPSSQAG-FTCECQEGWMGPLCDQRTNDPCLGNKCVHG-TCLPINAFSYSCKCLEGHGGVLCDEEE---DLFNPCQAIKCKHGKCRLSGLGQPYCECSSGYTGDSCDR 1449          
BLAST of slit1 vs. TrEMBL
Match: A1XCJ5 (Slit1 OS=Schmidtea mediterranea OX=79327 PE=2 SV=2)

HSP 1 Score: 2967.56 bits (7692), Expect = 0.000e+0
Identity = 1525/1534 (99.41%), Postives = 1528/1534 (99.61%), Query Frame = 1
Query:   43 MIFIFKDITFKFLLVLLSSLYCFSSTIWINDPSDESEICPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQLQCINDNIFNKLGMLKYLLLEGNKIFWISEAISKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCASEDKANLENMCPSDKKVCSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQIYNFQCNKEFESKYETHDEECVPKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLPPIYSIHEAPNLMKLDLSFNQIETLEPDVFTRSSKLREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQFHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCDGNASVYHSVDSCHETNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVLSFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNSIYFQKNSYIEISPPSRGFLMPRGNISLNFMTNSSSGLILYQGSEQTYLVVNLYQGHLRISYNLGDKVVGPEGYSMGMVNDSRLHALHIEIVGENLTMYLNGRVHCTLKSKYLNSNRYMALDTGIFIGGAPHKILQSAIMKFHIETDSEFIGCMESLLINRKKIDFSKYLPKSINVHPGCGKLISTVVASDLKIPSIMXXXXXXXXXXXXXXXNNVCKRPGICKNQGVCEVLPGSKKKFRCICKNGFVGRKCKKRKSMCHGTFTESHIVDPDSQGTCKSIERFQYRICTGTCSTSRMQSDVIKNFYHKPIDPSSSRCCQPTKYSTHEIQFKCENGRVYRRAANLPKNCKCVANCLND 4644
            MIFIFKDITFKFLLVLLSSLYCFSSTIWINDPSDESEICPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDNLVNLIKLRLNRNLIQYLPDDLFSKLTKLQKLDLRDNQLQCINDNIFNKLGMLKYLLLEGNKIFWISEAISKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTP  LSHIPISSLSSYEFQCASEDKANLENMCPSDKKVCSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGL+SLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQIYNFQCNKEFESKYETHDEECVPKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKL PIYSIHEAPNLMKLDLSFNQIETLEPDVFTRSSKLREI+LNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFKNNLNLKYLMLNKNQFHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCDGNASV HSVDSCHETNSVKTCCSIGNEECTKSKKCPSKCKCESTKVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLE LVLSFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNSIYFQKNSYIEISPPSRGFLMPRGNISLNFMTNSSSGLILYQGSEQTYLVVNLYQGHLRISYNLGDKVVGPEGYSMGMVNDSRLHALHIEIVGENLTMYLNGRVHCTLKSKYLNSNRYMALDTGIFIGGAPHKILQSAIMKFHIETDSEFIGCMESLLINRKKIDF+KYLPKSINVHPGCGKLISTVVASDLKIPSIMQPIKQILTQTTKKKPNNVCKRPGICKNQGVCEVLPGSKKK RCICKNGFVGRKCKKRKSMCHGTFTESHIVDPDSQGTCKSIERFQYRICTGTCSTSRMQSDVIKNFYHKPIDPSSSRCCQPTKYSTHEIQFKCENGRVYRRAANLPKNCKCVANCLND
Sbjct:    1 MIFIFKDITFKFLLVLLSSLYCFSSTIWINDPSDESEICPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDNLVNLIKLRLNRNLIQYLPDDLFSKLTKLQKLDLRDNQLQCINDNIFNKLGMLKYLLLEGNKIFWISEAISKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPXXLSHIPISSLSSYEFQCASEDKANLENMCPSDKKVCSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLISLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQIYNFQCNKEFESKYETHDEECVPKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLTPIYSIHEAPNLMKLDLSFNQIETLEPDVFTRSSKLREINLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFKNNLNLKYLMLNKNQFHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCDGNASVDHSVDSCHETNSVKTCCSIGNEECTKSKKCPSKCKCESTKVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLEILVLSFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNSIYFQKNSYIEISPPSRGFLMPRGNISLNFMTNSSSGLILYQGSEQTYLVVNLYQGHLRISYNLGDKVVGPEGYSMGMVNDSRLHALHIEIVGENLTMYLNGRVHCTLKSKYLNSNRYMALDTGIFIGGAPHKILQSAIMKFHIETDSEFIGCMESLLINRKKIDFTKYLPKSINVHPGCGKLISTVVASDLKIPSIMQPIKQILTQTTKKKPNNVCKRPGICKNQGVCEVLPGSKKKLRCICKNGFVGRKCKKRKSMCHGTFTESHIVDPDSQGTCKSIERFQYRICTGTCSTSRMQSDVIKNFYHKPIDPSSSRCCQPTKYSTHEIQFKCENGRVYRRAANLPKNCKCVANCLND 1534          
BLAST of slit1 vs. TrEMBL
Match: E1CHK5 (Slit OS=Dugesia japonica OX=6161 GN=Djslit PE=2 SV=1)

HSP 1 Score: 2213.73 bits (5735), Expect = 0.000e+0
Identity = 1120/1375 (81.45%), Postives = 1250/1375 (90.91%), Query Frame = 1
Query:   43 MIFIFKDITFKFLLVLLSSLYCFSSTIWINDPSDESEICPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQLQCINDNIFNKLGMLKYLLLEGNKIFWISEAISKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCASEDKANLENMCPSDKKVCSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQIYNFQCNKEFESKYETHDEECVPKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLPPIYSIHEAPNLMKLDLSFNQIETLEPDVFTRSSKLREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQFHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCDGNASVYHSVDSCHETNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVLSFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQS-EMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNSIYFQKNSYIEISPPSRGFLMPRGNISLNFMTNSSSGLILYQGSEQTYLVVNLYQGHLRISYNLGDKVVGPEGYSMGMVNDSRLHALHIEIVGENLTMYLNGRVHCTLKSKYLNSNRYMALDTGIFIGGAPHKILQSAIMKFHIETDSEFIGCMESLLINRKKIDFSKYLPKSINVHPGCGKLISTVVASDLKIPSI 4164
            M+ IFKDIT+KF L+LL SLYCFSSTIWINDPSDESE CPNGCQCENYSVECKHRLFPNVP+ILQEKTTRLNLLGNRINVIRKSDF KL  LKVLQLSNNRITSIEPGAFDNLVNLIKLRLNRNLI YLPD LFSKL+KLQKLDLRDNQLQCIN+N FNKL MLKYLLLEGNKIFWISE+ISKLK+LKELRI KNKL CNCHLAWLNWF+K + +I KP   SV+CS PFRLS IP+ +LS YEFQCASEDK NLEN+C  +KKVCSK+CNCS+DGIVKCVGK+LSNIPQDLP NVVELDL DNNITILQQGYLSKYK LKKI LT NGMVDIEPGAFLGL QLTS+YLN N LKTIRKNAF GL SLMFL+L  N+IECLP D  E  K+LH+LHLSENKLT+LK+ETF P + LR+LY+V+NPLNCDCR++WIVDYIK KE N P+A+C  PRN+QG  LR+LQ Y+FQC+ EF+SKYETH+EECVPKVSCPLKCTC+SD+VNCSN+GL SLP SIFPSTKTLIMSHNNL KL PIYS+HEAPNLMKLDLSFNQIE L+ DVF+RS+KL +I LNSN+LKCINNETFKNL +L  LQLS+N I+C+V DSFKNNLNLKYLMLNKN FHC+C +KW S+WSRNH+++LG+HRLPTCQSP +LK TP+THLD+KYFLCDGNAS+  S +SC+E +S + CCSIG++EC + KKCPSKCKCE TKVDCSD+QLKEIP+EIP+DTTELYLDRN L++LNETSFKNL+ LKTLVLSYNGITELNKNVLTPLK+LETLVLSFNKLQCIHQDAFK+L NLKVLILQSNDVSTIP+QAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFL+RFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKCD CMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNC+ +INACFGQPCRNGGICKNIDN+GNYECVCP+GFRGPKCT+N+DDCV + C+NG  C DL NSYSCKCQ+GFRGKYCD KF YC+DINPC+N G C  LPNNDYKC CSKGWEGK+C++N DDCKYN+CKHE++CVDLHNDYKC+CRPGY+GK+CE PAWKP  +   +SRR IN+S  M   + ENE+ISESVCAYHEC NNGIC++GDQNKNQKSYCKCKPG+SG LCQYLNS+YF KNSYIEI+PPSRGFLMP+GNISLNF+TNS+SGL+LYQG +QTYLVVNLYQGH+RISYNLGDKVVGPEGYSMGMVNDS+LH LHIEIVGENLTMYLNGRVHCTLKSKY+NSNR+MALDTGIFIGGAP+KILQSAI K+HI+TDSEFIGC+ESLLIN K+IDFSKYL +SINV PGCG L+STV+ SD++IPSI
Sbjct:    1 MLIIFKDITYKFYLLLLYSLYCFSSTIWINDPSDESETCPNGCQCENYSVECKHRLFPNVPIILQEKTTRLNLLGNRINVIRKSDFFKLIYLKVLQLSNNRITSIEPGAFDNLVNLIKLRLNRNLIHYLPDGLFSKLSKLQKLDLRDNQLQCINENTFNKLTMLKYLLLEGNKIFWISESISKLKNLKELRIKKNKLTCNCHLAWLNWFMKHRPDIFKPDVPSVVCSAPFRLSDIPVYTLSPYEFQCASEDKTNLENICQQNKKVCSKQCNCSNDGIVKCVGKQLSNIPQDLPVNVVELDLSDNNITILQQGYLSKYKTLKKINLTGNGMVDIEPGAFLGLQQLTSLYLNANKLKTIRKNAFSGLSSLMFLFLHGNEIECLPADAFEGVKKLHILHLSENKLTTLKRETFNPLIKLRFLYIVNNPLNCDCRLAWIVDYIKGKENNEPIASCHYPRNMQGLALRSLQTYSFQCSNEFDSKYETHNEECVPKVSCPLKCTCTSDIVNCSNTGLSSLPSSIFPSTKTLIMSHNNLNKLTPIYSVHEAPNLMKLDLSFNQIEKLDADVFSRSTKLTKIHLNSNKLKCINNETFKNLIDLRTLQLSDNEINCVVVDSFKNNLNLKYLMLNKNPFHCNCNMKWTSDWSRNHHSILGDHRLPTCQSPGLLKGTPLTHLDEKYFLCDGNASIDQSFESCNEISSTEACCSIGSDECKQIKKCPSKCKCEITKVDCSDMQLKEIPHEIPQDTTELYLDRNDLKTLNETSFKNLVFLKTLVLSYNGITELNKNVLTPLKNLETLVLSFNKLQCIHQDAFKDLKNLKVLILQSNDVSTIPFQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLERFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKCDGCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCENKINACFGQPCRNGGICKNIDNFGNYECVCPLGFRGPKCTLNIDDCVAHQCINGE-CVDLRNSYSCKCQNGFRGKYCDTKFSYCHDINPCKNHGVCHSLPNNDYKCTCSKGWEGKECDQNIDDCKYNTCKHEAKCVDLHNDYKCLCRPGYVGKYCEQPAWKP--ISKPLSRRYINESIIMIQSSSENEIISESVCAYHECSNNGICVSGDQNKNQKSYCKCKPGFSGPLCQYLNSLYFHKNSYIEINPPSRGFLMPKGNISLNFLTNSTSGLLLYQGFDQTYLVVNLYQGHVRISYNLGDKVVGPEGYSMGMVNDSKLHKLHIEIVGENLTMYLNGRVHCTLKSKYINSNRHMALDTGIFIGGAPNKILQSAITKYHIDTDSEFIGCIESLLINEKRIDFSKYLSRSINVKPGCGDLLSTVITSDMEIPSI 1372          
BLAST of slit1 vs. TrEMBL
Match: A0A4E0S2Z5 (Axon guidance protein OS=Fasciola hepatica OX=6192 GN=D915_002598 PE=4 SV=1)

HSP 1 Score: 909.827 bits (2350), Expect = 0.000e+0
Identity = 583/1718 (33.93%), Postives = 842/1718 (49.01%), Query Frame = 1
Query:  157 CPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQLQCINDNIFNKLGMLKYLLLEGNKIFWIS-EAISKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCST------------PFRLSH---IPISSLSSYEFQCASEDKANLENMCPSDKKVCSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETN-TPVAACLQPRNLQGTPLRNLQIYNFQCNKEFESKYETHDEEC--VPKVSCPLKCTC-----------SSDV---------------VNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLPPIYSIHEAPNLMKLDLSFNQIETLEPDVFTRSSKLREISLNSN------------------------RLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQFHCDCKLKWMSEWSRNHYT-VLGNHRLPTCQSPIILKDTPITHLDDKYFLCDGNASVYHSVDSCHE------------TNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVLSFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFIC----------SEETEDA---------------------------------------------YITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRR--------DINQSEMTYENLENEVIS----ESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNSIYF-QKNSYIEISPPSRGFLMPRGNISLNFMTNSSSGLILYQGS---------EQTYLVVNLYQGHLRISYNLGDKVVGPEGYSMGMVNDSRLHALHIEIVGENLTMYLN-----GRVHCTLKSKYLNSN-------------RYMALDTGIFIGGAPHKILQSAIMKFHIETDSEFIGCMESLLINRKKIDFSKYLPK-------------------------SINVHPGC---GKLISTVVASDLKIPSIMXXXXXXXXXXXXXXXNNVCKRPGICKNQGVCEVLPGSKKKFRCICKNGFVGRKCKKRKSMCHGTFTESHIVDPDSQGTCKSIERFQYRICTGTCS----------------TSRMQSDVIKNFY-------HKPIDPSSSRCCQPTKYSTHEIQFKCENGRVYRRAANLPKNCKCV 4626
            CP GC C +  VECK+  +  +P  +   T +L + GN ++ +  S F  L NLK L L+NN+I ++    F NL  L +LRLN+N +  LP+D+F  LT+LQ+LDLR+N+L C+  NIF  L  L+Y+ L GN I+WIS ++ S   HLK+L +  N   C+CH+  L  F    + +         C T               L H    P   L      CA +  A+           C   C+C+ D    C  + L +IPQDLP +++E++L  N +  L +     Y  L+KI+L  N +  ++  AF GL +L+S+Y++ N L+ +    F GL +L  LYL  N I CL + + E  + L +LHLSEN++ +  + +F     L+YLYLVHNPL CDCRM+W+  ++ +KE   T  A C +P  +QG  +R+L      C+    + +    EE   + K+ CP KC C           SSDV               V+CS +GL  LPE++  +TK L + +N +++L     + E P L  L L  N+IE ++   F  + +L+++ L++N                         L C+NN TF     L IL L+ N I CI   SF         +      +CDC L W+ +W R+H   V+     PTC  P  L  TP+  L   +F C+           CH             ++S  +CC+   +       CP  C CE+  V CSDL L EIP  IP  TT+LYL RN +++L+ +   +L  L TLVLSYN + EL +NV   LK+L+TLVLS N++QCIH +AF  L  L+VLILQ+N++S+IPY  FN L  LNNIALGQNP HCDCN KWLN FF  +FLDNGI+LC SPE M+ KS+YHSKP DF+C          S  TED                                               + +KC  CM  PC + G+C+     ++QC+CP P+HG+ C+   NAC   PC+NGG+C+ I     Y C+C  GF+G +C  ++  C  + C NG  C  +NN   C C +GF G  C   F+YC D+NPC N G+C  LP N Y+C C+KGW GK C+ N DDC YN C++ + CVD  N+Y C CRPGY+G +CE P W P    T + RR          + SE+ +  ++   +S    E++CAYH+C N   C+    +  Q   C C PG+SG  C++L SI     +SYI I PPSRG L+PRG ISL   T SSSG+++Y             + YL+V+L +GH+R+ + L  K    E  S+  +ND   H + I +  +NLT+ ++        +   KS Y   N             R++AL   ++ GGAP   L++A    + +      GC+E + IN + ID ++                             +I + PGC    K+  T V+       +        +  T++  +  C  P +C N G C  L      ++C C +GF G  C+K +S CH +   S I DP  Q +C S  +   R C G+CS                T   Q+D  ++ +       H+  +     CCQP K     + F+C +G  Y R   L K C C+
Sbjct:  139 CPPGCVCFSKDVECKYANWKQIPHGIPIHTEKLLITGNILSTLNASTFEGLVNLKSLILTNNQIRAVHEETFANLPRLRRLRLNQNQLSNLPEDVFRGLTQLQRLDLRENKLACLPPNIFKGLLELRYINLSGNNIYWISGDSFSDQSHLKQLDLTGNPFKCDCHIRPLIAFFLSTNQLPLLARSGATCHTHEGEVGQSSQTIGLHLLHQNNCPEGDLPKTHVACAEQAPAS---------SSCLSVCDCTVDRAANCQRRGLKSIPQDLPPDLMEINLAHNELESLPEMAFKNYPKLRKIVLDNNQITHVDGSAFNGLHELSSLYMSSNKLQALSPGLFLGLNNLKILYLHRNKISCLQQGSFEGLENLQILHLSENRIQTFPRGSFTDLRRLKYLYLVHNPLICDCRMAWLPAFLVEKEAGGTQWARCAEPLEVQGRHVRDLLPTLMSCDDSLINSHSAEGEESCDILKLKCPEKCRCTEITVTPKIQASSDVPFQLDELGLISDGLSVDCSEAGLTELPENMPVNTKDLNLRNNRIRELKADSILTELPQLNTLMLDGNRIEKIDSLAFRANRELKKLILSNNSLGSFDHRILQGMTKLQVLLLQHNELTCLNNMTFGTTSALRILMLNNNKIRCITKGSFDTMRLDSLSLSAN-PLNCDCHLSWLPDWLRDHVNQVIAGPLPPTCVEPEQLAGTPVASLARYHFTCNNTPG-----SGCHSGLGSGPPGSSPCSDSTASCCN-EEQAPVPQFGCPVGCNCEADGVYCSDLGLAEIPAGIPPGTTQLYLGRNQIKTLDPSRIGHLKKLHTLVLSYNKLRELPENVFESLKNLKTLVLSHNEIQCIHPNAFNGLSGLRVLILQANNISSIPYGTFNGLLQLNNIALGQNPLHCDCNGKWLNSFFRQQFLDNGIALCHSPENMRFKSLYHSKPGDFVCPWIRRTGQNQSSPTEDMATALFVDEVASESGTSPHEPRGWGTVRSVSSDDVARMSPEERASLAVASKCLPCMLNPCTNGGQCAARGQLDFQCNCPTPYHGRRCELEQNACSRNPCKNGGLCEVISLPSEYRCICSKGFQGERCEESIKVCSNHYCDNGGICEVVNNEQRCHCAAGFHGPRCQYAFVYCKDLNPCLNGGKCIALPENKYRCECTKGWTGKNCQTNADDCLYNRCENGASCVDGINEYICKCRPGYVGIYCERPTWNPDSYVTRMDRRIPARHLLKGFSSSEVRHSPIKIRSLSTPPLETLCAYHQCQNKATCVESHGDVYQ---CVCNPGFSGQYCEHLFSISLPSTHSYIAIVPPSRGALVPRGTISLKVSTRSSSGILVYYADLIKTGKNELPEQYLLVDLEEGHVRVMFALA-KDSNVERRSVFKINDGHAHTIQIRVESDNLTLTVDKVELPNNSYQPKKSAYPLPNEPESRTRENVTVLRHLALTNPLYFGGAPADRLEAAGRILNKDPVLGMTGCIEEVQINERPIDLARLFGAPVDKMTGAQESHSERPSAEAKQHGTAIGILPGCYTKPKMSQTAVSGSRTTELVGGEQWNSASDPTERSESTNCANPKVCLNGGTCVRLSTDTTHYKCECADGFTGPHCEKEQS-CHRSTQRSFIRDP--QTSCISTRKLTVRTCGGSCSGTGSDEDSTSEGLNDLTYTEQADATRSLWLPESIRRHRRSEKEDKICCQPIKVRYRRVHFQCVDGSAYERTVKLIKRCGCI 1833          

HSP 2 Score: 146.747 bits (369), Expect = 3.039e-31
Identity = 131/489 (26.79%), Postives = 203/489 (41.51%), Query Frame = 1
Query: 1513 VSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLPPIYSIHEAPNLMKLDLSFNQIET------------------------LEPDVFTRSSKLREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQFHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCDGNASVYHSV--DSCHETNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVLSFNKLQ------------------------CIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNG-ISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKCDSC--MKRPCKHNGKCSLIT 2820
            + CP  C C S  V C  +    +P  I   T+ L+++ N L  L    +     NL  L L+ NQI                          L  DVF   ++L+ + L  N+L C+    FK L EL  + LS N I  I  DSF +  +LK L L  N F CDC ++ +  +            L T Q P++ +     H  +            H +  ++C E +  KT  +   +    S              +C    LK IP ++P D  E+ L  N L+SL E +FKN   L+ +VL  N IT ++ +    L  L +L +S NKLQ                        C+ Q +F+ L NL++L L  N + T P  +F DL+ L  + L  NP  CDC + WL  F +++       + CA P +++ + +    PT   C +   +++     +SC  +K  C    +C+ IT
Sbjct:  137 IYCPPGCVCFSKDVECKYANWKQIPHGIPIHTEKLLITGNILSTL-NASTFEGLVNLKSLILTNNQIRAVHEETFANLPRLRRLRLNQNQLSNLPEDVFRGLTQLQRLDLRENKLACLPPNIFKGLLELRYINLSGNNIYWISGDSFSDQSHLKQLDLTGNPFKCDCHIRPLIAFF-----------LSTNQLPLLARSGATCHTHEGEVGQSSQTIGLHLLHQNNCPEGDLPKTHVACAEQAPASSSCLSVCDCTVDRAANCQRRGLKSIPQDLPPDLMEINLAHNELESLPEMAFKNYPKLRKIVLDNNQITHVDGSAFNGLHELSSLYMSSNKLQALSPGLFLGLNNLKILYLHRNKISCLQQGSFEGLENLQILHLSENRIQTFPRGSFTDLRRLKYLYLVHNPLICDCRMAWLPAFLVEKEAGGTQWARCAEPLEVQGRHVRDLLPTLMSCDDSLINSHSAEGEESCDILKLKCPEKCRCTEIT 613          
BLAST of slit1 vs. TrEMBL
Match: A0A267F2C1 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig016971g2 PE=4 SV=1)

HSP 1 Score: 867.455 bits (2240), Expect = 0.000e+0
Identity = 522/1546 (33.76%), Postives = 803/1546 (51.94%), Query Frame = 1
Query:  157 CPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQL------------------------QCINDNIFNKLGMLKYLLLEGNKIFWISE-AISKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCASEDKANLENMCP-SDKKVCSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDK-ETNTPVAACLQPRNLQGTPLRNLQIYNFQCNKEFESKYETHDEECVPKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLPPIYSIHEAPNLMKLDLSFNQIETLEPDVFTRSSKLREISLNSNRLK------------------------CINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQFHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCDGNASVYHSVDSCHETNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVLSFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNS--CKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNSIYFQKNSYIEISPPSRGFLMPRGNISLNFMTNSSSGLILYQGSEQTYLVVNLYQGHLRISYNLGDKVVGPEGYSMGMVNDSRLHALHIEIVGENLTMYLNGRVHCTLKSKYLN---SNRYMALDTGIFIGGAPHKILQSAIMKFHIETDSEFIGCMESLLINRKKIDFSKYLPKSINVHPGCGKLISTVVASDLKIPSIMXXXXXXXXXXXXXXXNNVCKRPGICKNQGVCEVLPGSKKKFRCICKNGFVGRKCKKRKSMCHGTFTESHIVDPDSQGTCKSIERFQYRICTGTCSTSRMQSDVIKNFYHKPIDPSSSRCCQPTKYSTHEIQFKCENGRVYRRAANLPKNCKCV 4626
            CP GC C   SV C       +P  L +   RLNL  NRI++IR+SDF  L  L++LQL NNRI  IEPG+FD L  L +LRLNRN I  LPD LF++ + L +LDL  N+L                        +C+ ++    L  L+ L L  N I  + E A S++  LK LR+  N   C+C LAWL+ +L+Q+  +   G H+  C+ P  L    ++ LSS +FQC+++      + C  +++  C +KC C  D  V+C  K LS +P+D+PTNV EL L++N IT++++G   +   L+++ L  N +  +E GAF GL  L S+ L+ N+++ + +  F GL  L  L L  N + C+P+D    ++ L +L L +N++ S+ + TF P  +++ L +  NP  CDC + W+ D++++     +    C  P  +    L  +    F+C ++ ES+   +   C     CP  C C  D V+CS   L SLP+++  +T+ L +S N ++ +P    + +  NL  +DLS N I T+EPD F  +S L  ++L+ N+L+                        C+N++TF+    + +L +S+N I C++  +F    NL  L L+ N  HC+C +KW+  ++  ++ ++ N + P C+SP +LKDTP+  L  ++F+C  + S+      C     V  CCS     C     CP KC+CE + V C    L E+P+ IP D+T+L+L +N ++ LN T    L NLK L LS+N +  L  N+   + +LE+L +SFNKL C+   AF  L  LKVLILQ ND++++P+ +F+DL  L  I L QNP HCDCN+KWL  FF  R LDNG+SLCASP  + +K IYH++P DF+C+  T+  +  +KC+ C   PC + G C  +++  + CDCP  F GK C  R++ACFG PC N   C+ +  +G Y C C +GF G  C  N+DDC+G+ C N ATC D  N+Y+C C  GF G+YC+ +  YC D NPCQN G+C   P   Y+C C  GWEG+ C+ N D+C+  +  C +   C+D    Y C CRPGY G+ CE       ++                 ++ +  ++  VC +HECLN G CI     +  +  C+C  GY G  C+ L  + F  +S+  I       + P GNI++   T    GL+LY G+   YL+++L+ G++R SY+LG    G  GY+   +ND + H +   I  E +T+ ++G    T+ + Y+    +    AL   I+IG  P           +I     F GC++ +L+N K ++F    P +  +  GC                                  N C++P +CKN+GVC+        +RC C+  F GR C+++   C    +  +  DP++   C+S  + + R C GTC                    S+  CCQP +     I+F C  GR   +   L + C C 
Sbjct:   58 CPVGCGCSGRSVSCSKAGLTTIPTELPDNVERLNLQSNRISLIRRSDFSGLTKLRILQLQNNRIERIEPGSFDELTQLERLRLNRNRITELPDGLFTRQSNLYRLDLSYNRLTFIGKDTLSGAPRLRNLQLDHNDIRCLTESALRGLERLEVLTLNRNNISTLPENAFSRMARLKTLRLADNNFICDCRLAWLSRWLRQQPQL---GLHTK-CAQPRLLFGSQVAELSSSDFQCSADHHKLYPSACAMAERANCPRKCYCQEDN-VRCNQKDLSEVPEDIPTNVQELHLENNKITLIRRGTFRRLTQLRRLFLNGNQISRLEEGAFEGLDNLYSLVLSRNSIRELPRGIFQGLKFLQLLLLDANKLTCIPEDIFHETRNLAILSLYKNEIKSIAETTFAPLRSIQQLTIAGNPFVCDCNLRWLNDFLREHPRLASSDNVCDSPDRMARKKLGEVASRKFRC-RDGESEATRNAGRCELYAECPANCKCEGDTVDCSWKRLSSLPKNLPVATRHLKLSDNMIRDVPVNSILKKLKNLQSIDLSNNLIATIEPDSFRENSNLEVLNLSGNKLRSLDPRMLSGLDKLKILQMSNNHLSCLNSDTFQETPNITVLSVSQNQIRCVMEGTFDTLQNLTSLHLDLNPLHCNCHMKWLPPFAVANHQLMRNSQ-PLCRSPDLLKDTPVVSLQSRHFVCQQDPSM-----GCQ---GVPPCCSGDAVSCDHRAFCPKKCECEGSVVRCIGQGLTEVPDHIPVDSTQLFLGQNMIEQLNFTRLNRLRNLKLLDLSHNQLYGLKSNMFANMSNLESLSVSFNKLACVEARAFAGLSKLKVLILQGNDINSLPFGSFDDLSQLTTIGLNQNPIHCDCNVKWLIDFFQRRGLDNGLSLCASPPDLAMKPIYHARPDDFVCTTATQLNH--SKCNICSTEPCLNGGTCRTVSYDRFACDCPAGFEGKRCQNRLDACFGSPCHNQAECE-VLRFGRYRCRCKIGFSGTHCETNIDDCIGHRCQNNATCLDGVNNYTCNCSPGFTGRYCETRLHYCTDYNPCQNGGECVTDPATGYRCKCVTGWEGRNCDINPDNCRRQTQPCMNGGSCIDGIGSYTCQCRPGYTGRLCERLEMASLIM-----------------DVPDAAVNRGVCTHHECLNGGRCI----QEGSEYRCQCLAGYEGKKCEKLTQVGFYDDSFARIETSR---IYPSGNITMEMATQQRQGLLLYAGNASAYLIMSLFNGYIRFSYSLG-VAPGANGYTYVKINDGKFHTVQAIINRETVTLSVDGMAK-TISATYVADAVARSVDALSEAIYIGTLPAAKRDDMQSNVNIRETFSFGGCVKRMLVNGKLVNFKLSDPTNRRLIAGCPAFAKPA---------------------------NPCEKPNLCKNRGVCQPRVEQPAGYRCKCRPNFTGRNCEEK--ACKRLASREYYTDPET--GCRSRRKLKIRRCQGTCLR------------------STGYCCQPKRLRHRMIKFLCPGGRRITKRVTLVRRCGCA 1510          

HSP 2 Score: 65.855 bits (159), Expect = 9.865e-7
Identity = 57/222 (25.68%), Postives = 101/222 (45.50%), Query Frame = 1
Query:  142 DESEICPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQLQCINDNIFNKLGMLKYLLLEGNKIFWIS-EAISKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCASEDKAN 804
            D    CP  C+CE   V C  +    VP  +   +T+L L  N I  +  +   +L NLK+L LS+N++                          L  ++F+ ++ L+ L +  N+L C+    F  L  LK L+L+GN I  +   +   L  L  + +++N ++C+C++ WL  F +++   +  G    LC++P  L+  PI      +F C +  + N
Sbjct:  764 DHRAFCPKKCECEGSVVRCIGQGLTEVPDHIPVDSTQLFLGQNMIEQLNFTRLNRLRNLKLLDLSHNQLYG------------------------LKSNMFANMSNLESLSVSFNKLACVEARAFAGLSKLKVLILQGNDINSLPFGSFDDLSQLTTIGLNQNPIHCDCNVKWLIDFFQRRG--LDNGLS--LCASPPDLAMKPIYHARPDDFVCTTATQLN 957          
BLAST of slit1 vs. TrEMBL
Match: A0A267F5J3 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig016971g1 PE=4 SV=1)

HSP 1 Score: 863.218 bits (2229), Expect = 0.000e+0
Identity = 521/1546 (33.70%), Postives = 802/1546 (51.88%), Query Frame = 1
Query:  157 CPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQL------------------------QCINDNIFNKLGMLKYLLLEGNKIFWISE-AISKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCASEDKANLENMCP-SDKKVCSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDK-ETNTPVAACLQPRNLQGTPLRNLQIYNFQCNKEFESKYETHDEECVPKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLPPIYSIHEAPNLMKLDLSFNQIETLEPDVFTRSSKLREISLNSNRLK------------------------CINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQFHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCDGNASVYHSVDSCHETNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVLSFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNS--CKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNSIYFQKNSYIEISPPSRGFLMPRGNISLNFMTNSSSGLILYQGSEQTYLVVNLYQGHLRISYNLGDKVVGPEGYSMGMVNDSRLHALHIEIVGENLTMYLNGRVHCTLKSKYLN---SNRYMALDTGIFIGGAPHKILQSAIMKFHIETDSEFIGCMESLLINRKKIDFSKYLPKSINVHPGCGKLISTVVASDLKIPSIMXXXXXXXXXXXXXXXNNVCKRPGICKNQGVCEVLPGSKKKFRCICKNGFVGRKCKKRKSMCHGTFTESHIVDPDSQGTCKSIERFQYRICTGTCSTSRMQSDVIKNFYHKPIDPSSSRCCQPTKYSTHEIQFKCENGRVYRRAANLPKNCKCV 4626
            CP GC C   SV C       +P  L +   RLNL  NRI++IR+SDF  L  L++LQL NNRI  IEPG+FD L  L +LRLNRN I  LPD LF++ + L +LDL  N+L                        +C+ ++    L  L+ L L  N I  + E A S++  LK LR+  N   C+C LAWL+ +L+Q+  +   G H+  CS P  L    ++ LSS +FQC+++      + C  +++  C ++C C  D  V+C  K LS +P+D+PTNV EL L++N IT++++G   +   L+++ L  N +  +E GAF GL  L S+ L+ N+++ + +  F GL  L  L L  N + C+P+D    ++ L +L L +N++ S+ + TF P  +++ L +  NP  CDC + W+ D++++     +    C  P  +    L  +    F+C ++ ES+   +   C     CP  C C  D V+CS   L SLP+++  +T+ L +S N ++ +P    + +  NL  +DLS N I T+EPD F  +S L  ++L+ N+L+                        C+N++TF+    + +L +S+N I C++  +F    NL  L L+ N  HC+C +KW+  ++  ++ ++ N + P C+SP +LKDTP+  L  ++F+C  + S+      C     V  CCS     C     CP KC+CE + V C    L E+P+ IP D+T+L+L +N ++ LN T    L NLK L LS+N +  L  N+   + +LE+L +SFNKL C+   AF  L  LKVLILQ ND++++P+ +F+DL  L  I L QNP HCDCN+KWL  FF  R LDNG+SLCASP  + +K IYH++P DF+C+  T+  +  +KC+ C   PC + G C  +++  + CDC   F GK C  R++ACFG PC N   C+ +  +G Y C C +GF G  C  N+DDC+G+ C N ATC D  N+Y+C C  GF G+YC+ +  YC D NPCQN G+C   P   Y+C C  GWEG+ C+ N D+C+  +  C +   C+D    Y C CRPGY G+ CE       ++                 ++ +  ++  VC +HECLN G CI     +  +  C+C  GY G  C+ L  + F  +S+  I       + P GNI++   T    GL+LY G+   YL+++L+ G++R SY+LG    G  GY+   +ND + H +   I  E +T+ ++G    T+ + Y+    +    AL   I+IG  P           +I     F GC++ +L+N K ++F    P +  +  GC                                  N C++P +CKN+GVC+        +RC C+  F GR C+++   C    +  +  DP++   C+S  + + R C GTC                    S+  CCQP +     I+F C  GR   +   L + C C 
Sbjct:   58 CPVGCGCSGRSVSCSKAGLTTIPTELPDNVERLNLQSNRISLIRRSDFSGLTKLRILQLQNNRIERIEPGSFDELTQLERLRLNRNRITELPDGLFTRQSNLYRLDLSYNRLTFIGKDTLSGAPRLRNLQLDHNDIRCLTESALRGLERLEVLTLNRNNISTLPENAFSRMARLKTLRLADNNFICDCRLAWLSRWLRQQPQL---GLHTK-CSQPRLLFGSQVAELSSSDFQCSADHHKLYPSACAMAERANCPRQCYCQEDN-VRCNQKDLSEVPEDIPTNVQELHLENNKITLIRRGTFRRLTQLRRLFLNGNQISRLEEGAFEGLDNLYSLVLSRNSIRELPRGIFQGLKFLQLLLLDANKLTCIPEDIFHETRNLAILSLYKNEIKSIAETTFAPLRSIQQLTIAGNPFVCDCNLRWLNDFLREHPRLASSDNVCDSPDRMARKKLGEVASRKFRC-RDGESEATRNAGRCELYAECPANCKCEGDTVDCSWKRLTSLPKNLPVATRHLKLSDNMIRDVPVNSILKKLKNLQSIDLSNNLIATIEPDSFRENSNLEVLNLSGNKLRSLDPRMLSGLDKLKILQMSNNHLSCLNSDTFQETPNITVLSVSQNQIRCVMEGTFDTLQNLTSLHLDLNPLHCNCHMKWLPPFAVANHQLMRNSQ-PLCRSPDLLKDTPVVSLQSRHFVCQQDPSM-----GCQ---GVPPCCSGDAVSCDHRAFCPKKCECEGSVVRCIGQGLTEVPDHIPVDSTQLFLGQNLIEQLNFTRLNRLRNLKLLDLSHNQLYGLKSNMFANMSNLESLSVSFNKLACVEAGAFAGLSKLKVLILQGNDINSLPFGSFDDLSQLTTIGLNQNPIHCDCNVKWLIDFFQRRGLDNGLSLCASPPDLAMKPIYHARPDDFVCTTATQLNH--SKCNICSTEPCLNGGTCRTVSYDRFACDCQAGFEGKRCQNRLDACFGSPCHNQAECE-VLRFGRYRCRCKIGFSGTHCETNIDDCIGHRCQNNATCLDGVNNYTCNCSPGFTGRYCETRLHYCTDYNPCQNGGECVTDPATGYRCKCVTGWEGRNCDINPDNCRRQTQPCMNGGSCIDGIGSYTCQCRPGYTGRLCERLEMASLIM-----------------DVPDAAVNRGVCTHHECLNGGRCI----QEGSEYRCQCLAGYEGKKCEKLTQVGFYDDSFARIETSR---IYPSGNITMEMATQQRQGLLLYAGNASAYLIMSLFNGYIRFSYSLG-VAPGANGYTYVKINDGKFHTVQAIINRETVTLSVDGMAK-TISATYVADAVARSVDALSEAIYIGTLPAAKRDDMQSNVNIRETFSFGGCVKRMLVNGKLVNFKLSDPTNRRLIAGCPAFAKP---------------------------ENPCEKPNLCKNRGVCQPRVEQPAGYRCKCRPNFTGRNCEEK--ACKRLASREYYTDPET--GCRSRRKLKIRRCQGTCPR------------------STGYCCQPKRLRHRMIKFLCPGGRRITKRVTLVRRCGCA 1510          

HSP 2 Score: 65.4698 bits (158), Expect = 1.292e-6
Identity = 57/222 (25.68%), Postives = 101/222 (45.50%), Query Frame = 1
Query:  142 DESEICPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQLQCINDNIFNKLGMLKYLLLEGNKIFWIS-EAISKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCASEDKAN 804
            D    CP  C+CE   V C  +    VP  +   +T+L L  N I  +  +   +L NLK+L LS+N++                          L  ++F+ ++ L+ L +  N+L C+    F  L  LK L+L+GN I  +   +   L  L  + +++N ++C+C++ WL  F +++   +  G    LC++P  L+  PI      +F C +  + N
Sbjct:  764 DHRAFCPKKCECEGSVVRCIGQGLTEVPDHIPVDSTQLFLGQNLIEQLNFTRLNRLRNLKLLDLSHNQLYG------------------------LKSNMFANMSNLESLSVSFNKLACVEAGAFAGLSKLKVLILQGNDINSLPFGSFDDLSQLTTIGLNQNPIHCDCNVKWLIDFFQRRG--LDNGLS--LCASPPDLAMKPIYHARPDDFVCTTATQLN 957          
BLAST of slit1 vs. Ensembl Cavefish
Match: slit3 (slit guidance ligand 3 [Source:NCBI gene;Acc:103037708])

HSP 1 Score: 695.271 bits (1793), Expect = 0.000e+0
Identity = 484/1553 (31.17%), Postives = 741/1553 (47.71%), Query Frame = 1
Query:  157 CPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQLQCINDNIFNKLGMLKYLLLEGNKIFWIS-EAISKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCASEDKANLENMCPSDKKVCSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQIYNFQC--NKEFESKYETHDEECVPKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLPPIYSIHEAPNLMKLDLSFNQIETLEPDVFTRS------------------------SKLREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQFHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCDG---NA---SVYHSVDSCHETNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVLSFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICK-NIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYC-NDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNSIYF-QKNSYIEISPPSRGFLMPRGNISLNFMTNSSSGLILYQGSEQTYLVVNLYQGHLRISYNLGDKVVGPEGYSMGMVNDSRLHALHIEIVGENLTMYLNGRVHCTLKSKYLNSNRYMALDTGIFIGGAPHKILQSAIMKFHIETDSEFIGCMESLLINRKKIDFSKYLPKSINVHPGCGKLISTV---------VASDLKIPSIMXXXXXXXXXXXXXXXNNVCKRPGICKNQGVCEVLPGSKKKFRCICKNGFVGRKCKKRKS--MCHGTFTESH--IVDPDSQGTCKSIERFQYRICTG--TCSTSRMQSDVIKNFYHK-------------PIDPSSSRCCQPTKYSTHEIQFKCENGRVYRRAANLPKNCKC 4623
            CP  C C    VEC+ + F +VP  +     RL++  N I  I K DF  L NL+VL L NN+I+ IE GAF +L  L +LRLNRN +Q LP+ LF    KL +L L  N + CI D  F  L  L+ L L  N I  I   + + +  L+ LR+H N L+C+CHL+WL+ +L+Q+  +    AH   C +P  +  + +  +   +F C    ++   + C     VC   C+C+++ IV C  K L+ IP +LP  +VE+ L+ N I  +  G  S YK LK+I L++N + DI   AF GL  LTS+ L  N +  I K  F GLVSL  L L  N I CL  +T +  + L+LL L +NKL ++ K  F P   ++ L+L  NP  CDC + W+ DY+ D    T  A C  PR L    +  ++   F+C  ++++ S+      EC   + CP +C C   VV+CSN  L S+P  +   T  L ++ N +  L    +  + PNL K++LS N++  +   VF  +                        + L+ + L SN++ C++N TF  L  + +L L +N IS I   +F    +L  + L  N + CDC L W+  W +    V GN   P CQ P  LK+ PI  +    F CDG   NA   SV    D C  +++V                           V CS+  L+ +P  IPKDTTELYL+ N L S+ +    +L  L  + LS N I+ L   + + +  L TL+LS+N+++C+   AF  L +L++L L  ND+STIP  AFN L +L+++ALG NP +CDC ++WL+Q+    F + GI+ C  P  M  + +  +    F C     D  + +KC  C+  PC++NG C      +Y C CP+ + G+NC+   NAC  QPC NGG C       G++ CVCP GF G +C +N +DC  N C N +TC D  N+Y+C C   +RG  C+     C    +PCQ+  +C  L    Y+C C  G+ G+ CE++ +DC  N C+H ++CVD  N Y C+C+ G+ G FCE P   P +L                      +   S C   +C N   C+  +     +  C+C PG+ G+ CQ +++++F  K S+I++    R  ++   +ISL   T+  +G++LY+  +   L + LYQGH+R+ Y++  K      YS+  VND   H + + I  + L++ ++     +L    L     +  +T ++IGG P ++   +           F GC+ ++ IN +  +    L     + PGC                V    + P+           T+    N+ C   G C   G+          + C C  G+ G  C  R+    C G         VD   + TC     +    C    +C    ++  + ++  HK             P       CC  TK    +I FKC +G  +        +C C
Sbjct:   33 CPAKCSCSGSHVECQQQGFRSVPRGIPRNAERLDVDRNNITRITKVDFSGLKNLRVLHLENNQISVIERGAFQDLKQLERLRLNRNRLQVLPELLFQSTPKLGRLQLDSNHISCIEDGAFRALRDLEILTLNNNNITLIPLSSFNHMPKLRTLRLHSNNLHCDCHLSWLSDWLRQRKGL----AHFTQCMSPAHMRGLSVPDVQKRDFVCTGPAQSEPRS-CAPQASVCPASCSCTNN-IVDCRRKGLTEIPANLPEGIVEIRLEQNLIKSIPPGAFSPYKKLKRIDLSKNHISDIADDAFSGLRSLTSLVLYGNKIAEIPKGLFDGLVSLQLLLLNANKINCLRVNTFKDLQSLNLLSLYDNKLQTISKGLFTPLRAIKTLHLAQNPFMCDCHLKWLADYLFDNPIETSGARCSHPRRLANKRISQIKGKKFRCTGSEDYRSRLTG---ECFQDLVCPERCRCEGTVVDCSNLKLTSIPPHLPEHTTDLRLNDNEISSLDATGAFKKLPNLRKINLSNNKLRDIREGVFDGAGGVLELLLTGNKLQAVQGRMFRGLTGLKTLMLRSNQITCVDNATFTGLSSVRLLSLYDNRISTIAPGAFNTLHSLSTINLLSNPYVCDCHLAWLGLWLKKTRVVSGN---PRCQRPAFLKEIPIQDVAAPDFTCDGVEENACLPSVPRCPDPCTCSDTV---------------------------VRCSNHGLRSLPKGIPKDTTELYLEGNMLTSVPK-ELSSLKQLSLVDLSNNSISSLTPFIFSNMTQLSTLILSYNQIRCVPVHAFDGLRSLRLLTLHGNDLSTIPEGAFNHLTSLSHLALGANPLYCDCELRWLSQWVKAGFKEPGIARCTGPPDMADRLLLTTPLNRFQCKGPV-DLNLMSKCAPCLATPCQNNGTCVSDVTGSYHCTCPFGYKGRNCEIAANACLSQPCINGGTCHLTPGQEGHFSCVCPPGFEGQRCEVNPNDCEDNDCENNSTCVDGINNYTCVCPPNYRGDLCEEVVDACLPGFDPCQHDSKCVPL-TRGYRCECLPGYVGQHCEQDYNDCLENKCQHGAECVDAINGYTCVCKDGFSGLFCENPP--PMIL----------------------LQKTSPCDQSDCQNGAECVLME----GEPVCRCLPGFFGNKCQKMHTVHFLGKESFIQL---PRTKILSSTHISLQVATDKDNGMLLYK-EDHDPLAIELYQGHIRLMYDIA-KYPPTTVYSVESVNDGFFHTVELLIQNQTLSLVVDKGAPKSLGK--LPRPPTVETNTRLYIGGVPSELSSPSSRSGADRQSQSFNGCIHNVRINGELQELGSGLDSVKAILPGCHACSVCAQGACRQRDDVGVTCECPAGSTGPLCDQQGTSNPCQNSRCVH-GQCVANGL---------AYSCKCAQGYSGPYCATREEPLACRGLHCVHGECRVDESGEATCACQPGYTGPTCETELSCQGEMIREQLKRHMGHKTCTSTIKVPRMECPRVCHPGLCCTSTKSRRRKITFKCSDGFTFSEELETTLDCNC 1498          

HSP 2 Score: 283.108 bits (723), Expect = 7.516e-77
Identity = 207/653 (31.70%), Postives = 317/653 (48.55%), Query Frame = 1
Query:  139 SDESEICPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQLQCINDNIFNKLGMLKYLLLEGNKIFWISEAI-SKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQC-ASED-KANLENMCPSDKKVCSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQ-GYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQIYNFQCNKEFESKYETHDEECVPKVS-CPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLPPIYSIHEAPNLMKLDLSFNQIETLEPDVFTRSSKLREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQFHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCDG 2082
            + ++ +CP  C C N  V+C+ +    +P  L E    + L  N I  I    F     LK + LS N I+ I   AF  L +L  L L  N I  +P  LF  L  LQ L L  N++ C+  N F  L  L  L L  NK+  IS+ + + L+ +K L + +N   C+CHL WL  +L     I   GA    CS P RL++  IS +   +F+C  SED ++ L   C  D  VC ++C C    +V C   +L++IP  LP +  +L L DN I+ L   G   K   L+KI L+ N + DI  G F G   +  + L  N L+ ++   F GL  L  L L+ N I C+   T      + LL L +N+++++    F    +L  + L+ NP  CDC ++W+  ++K     +    C +P  L+  P++++   +F C+          +  C+P V  CP  CTCS  VV CSN GL SLP+ I   T  L +  N L  +P    +     L  +DLS N I +L P +F+  ++L  + L+ N+++C+    F  L+ L +L L  N +S I   +F +  +L +L L  N  +CDC+L+W+S+W +  +   G  R   C  P  + D  +       F C G
Sbjct:  250 APQASVCPASCSCTNNIVDCRRKGLTEIPANLPEGIVEIRLEQNLIKSIPPGAFSPYKKLKRIDLSKNHISDIADDAFSGLRSLTSLVLYGNKIAEIPKGLFDGLVSLQLLLLNANKINCLRVNTFKDLQSLNLLSLYDNKLQTISKGLFTPLRAIKTLHLAQNPFMCDCHLKWLADYLFDNP-IETSGAR---CSHPRRLANKRISQIKGKKFRCTGSEDYRSRLTGECFQD-LVCPERCRC-EGTVVDCSNLKLTSIPPHLPEHTTDLRLNDNEISSLDATGAFKKLPNLRKINLSNNKLRDIREGVFDGAGGVLELLLTGNKLQAVQGRMFRGLTGLKTLMLRSNQITCVDNATFTGLSSVRLLSLYDNRISTIAPGAFNTLHSLSTINLLSNPYVCDCHLAWLGLWLKKTRVVSGNPRCQRPAFLKEIPIQDVAAPDFTCDG-------VEENACLPSVPRCPDPCTCSDTVVRCSNHGLRSLPKGIPKDTTELYLEGNMLTSVP--KELSSLKQLSLVDLSNNSISSLTPFIFSNMTQLSTLILSYNQIRCVPVHAFDGLRSLRLLTLHGNDLSTIPEGAFNHLTSLSHLALGANPLYCDCELRWLSQWVKAGFKEPGIAR---CTGPPDMADRLLLTTPLNRFQCKG 884          
BLAST of slit1 vs. Ensembl Cavefish
Match: slit3 (slit guidance ligand 3 [Source:NCBI gene;Acc:103037708])

HSP 1 Score: 688.723 bits (1776), Expect = 0.000e+0
Identity = 487/1577 (30.88%), Postives = 744/1577 (47.18%), Query Frame = 1
Query:  157 CPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQLQ------------------------CINDNIFNKLGMLKYLLLEGNKIFWIS-EAISKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCASEDKANLENMCPSDKKVCSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQIYNFQC--NKEFESKYETHDEECVPKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLPPIYSIHEAPNLMKLDLSFNQIETLEPDVFTRS------------------------SKLREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQFHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCDG---NA---SVYHSVDSCHETNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVLSFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICK-NIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYC-NDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNSIYF-QKNSYIEISPPSRGFLMPRGNISLNFMTNSSSGLILYQGSEQTYLVVNLYQGHLRISYNLGDKVVGPEGYSMGMVNDSRLHALHIEIVGENLTMYLNGRVHCTLKSKYLNSNRYMALDTGIFIGGAPHKILQSAIMKFHIETDSEFIGCMESLLINRKKIDFSKYLPKSINVHPGCGKLISTV---------VASDLKIPSIMXXXXXXXXXXXXXXXNNVCKRPGICKNQGVCEVLPGSKKKFRCICKNGFVGRKCKKRKS--MCHGTFTESH--IVDPDSQGTCKSIERFQYRICTG--TCSTSRMQSDVIKNFYHK-------------PIDPSSSRCCQPTKYSTHEIQFKCENGRVYRRAANLPKNCKC 4623
            CP  C C    VEC+ + F +VP  +     RL++  N I  I K DF  L NL+VL L NN+I+ IE GAF +L  L +LRLNRN +Q LP+ LF    KL +LDL +NQ+Q                        CI D  F  L  L+ L L  N I  I   + + +  L+ LR+H N L+C+CHL+WL+ +L+Q+  +    AH   C +P  +  + +  +   +F C    ++   + C     VC   C+C+++ IV C  K L+ IP +LP  +VE+ L+ N I  +  G  S YK LK+I L++N + DI   AF GL  LTS+ L  N +  I K  F GLVSL  L L  N I CL  +T +  + L+LL L +NKL ++ K  F P   ++ L+L  NP  CDC + W+ DY+ D    T  A C  PR L    +  ++   F+C  ++++ S+      EC   + CP +C C   VV+CSN  L S+P  +   T  L ++ N +  L    +  + PNL K++LS N++  +   VF  +                        + L+ + L SN++ C++N TF  L  + +L L +N IS I   +F    +L  + L  N + CDC L W+  W +    V GN   P CQ P  LK+ PI  +    F CDG   NA   SV    D C  +++V                           V CS+  L+ +P  IPKDTTELYL+ N L S+ +    +L  L  + LS N I+ L   + + +  L TL+LS+N+++C+   AF  L +L++L L  ND+STIP  AFN L +L+++ALG NP +CDC ++WL+Q+    F + GI+ C  P  M  + +  +    F C     D  + +KC  C+  PC++NG C      +Y C CP+ + G+NC+   NAC  QPC NGG C       G++ CVCP GF G +C +N +DC  N C N +TC D  N+Y+C C   +RG  C+     C    +PCQ+  +C  L    Y+C C  G+ G+ CE++ +DC  N C+H ++CVD  N Y C+C+ G+ G FCE P     + KT                        S C   +C N   C+  +     +  C+C PG+ G+ CQ +++++F  K S+I++    R  ++   +ISL   T+  +G++LY+  +   L + LYQGH+R+ Y++  K      YS+  VND   H + + I  + L++ ++     +L    L     +  +T ++IGG P ++   +           F GC+ ++ IN +  +    L     + PGC                V    + P+           T+    N+ C   G C   G+          + C C  G+ G  C  R+    C G         VD   + TC     +    C    +C    ++  + ++  HK             P       CC  TK    +I FKC +G  +        +C C
Sbjct:   33 CPAKCSCSGSHVECQQQGFRSVPRGIPRNAERLDVDRNNITRITKVDFSGLKNLRVLHLENNQISVIERGAFQDLKQLERLRLNRNRLQVLPELLFQSTPKLGRLDLSENQIQAIPRKSFRGITNVKNLQLDSNHISCIEDGAFRALRDLEILTLNNNNITLIPLSSFNHMPKLRTLRLHSNNLHCDCHLSWLSDWLRQRKGL----AHFTQCMSPAHMRGLSVPDVQKRDFVCTGPAQSEPRS-CAPQASVCPASCSCTNN-IVDCRRKGLTEIPANLPEGIVEIRLEQNLIKSIPPGAFSPYKKLKRIDLSKNHISDIADDAFSGLRSLTSLVLYGNKIAEIPKGLFDGLVSLQLLLLNANKINCLRVNTFKDLQSLNLLSLYDNKLQTISKGLFTPLRAIKTLHLAQNPFMCDCHLKWLADYLFDNPIETSGARCSHPRRLANKRISQIKGKKFRCTGSEDYRSRLTG---ECFQDLVCPERCRCEGTVVDCSNLKLTSIPPHLPEHTTDLRLNDNEISSLDATGAFKKLPNLRKINLSNNKLRDIREGVFDGAGGVLELLLTGNKLQAVQGRMFRGLTGLKTLMLRSNQITCVDNATFTGLSSVRLLSLYDNRISTIAPGAFNTLHSLSTINLLSNPYVCDCHLAWLGLWLKKTRVVSGN---PRCQRPAFLKEIPIQDVAAPDFTCDGVEENACLPSVPRCPDPCTCSDTV---------------------------VRCSNHGLRSLPKGIPKDTTELYLEGNMLTSVPK-ELSSLKQLSLVDLSNNSISSLTPFIFSNMTQLSTLILSYNQIRCVPVHAFDGLRSLRLLTLHGNDLSTIPEGAFNHLTSLSHLALGANPLYCDCELRWLSQWVKAGFKEPGIARCTGPPDMADRLLLTTPLNRFQCKGPV-DLNLMSKCAPCLATPCQNNGTCVSDVTGSYHCTCPFGYKGRNCEIAANACLSQPCINGGTCHLTPGQEGHFSCVCPPGFEGQRCEVNPNDCEDNDCENNSTCVDGINNYTCVCPPNYRGDLCEEVVDACLPGFDPCQHDSKCVPL-TRGYRCECLPGYVGQHCEQDYNDCLENKCQHGAECVDAINGYTCVCKDGFSGLFCENPPPMILLQKT------------------------SPCDQSDCQNGAECVLME----GEPVCRCLPGFFGNKCQKMHTVHFLGKESFIQL---PRTKILSSTHISLQVATDKDNGMLLYK-EDHDPLAIELYQGHIRLMYDIA-KYPPTTVYSVESVNDGFFHTVELLIQNQTLSLVVDKGAPKSLGK--LPRPPTVETNTRLYIGGVPSELSSPSSRSGADRQSQSFNGCIHNVRINGELQELGSGLDSVKAILPGCHACSVCAQGACRQRDDVGVTCECPAGSTGPLCDQQGTSNPCQNSRCVH-GQCVANGL---------AYSCKCAQGYSGPYCATREEPLACRGLHCVHGECRVDESGEATCACQPGYTGPTCETELSCQGEMIREQLKRHMGHKTCTSTIKVPRMECPRVCHPGLCCTSTKSRRRKITFKCSDGFTFSEELETTLDCNC 1522          

HSP 2 Score: 282.722 bits (722), Expect = 1.011e-76
Identity = 207/653 (31.70%), Postives = 317/653 (48.55%), Query Frame = 1
Query:  139 SDESEICPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQLQCINDNIFNKLGMLKYLLLEGNKIFWISEAI-SKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQC-ASED-KANLENMCPSDKKVCSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQ-GYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQIYNFQCNKEFESKYETHDEECVPKVS-CPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLPPIYSIHEAPNLMKLDLSFNQIETLEPDVFTRSSKLREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQFHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCDG 2082
            + ++ +CP  C C N  V+C+ +    +P  L E    + L  N I  I    F     LK + LS N I+ I   AF  L +L  L L  N I  +P  LF  L  LQ L L  N++ C+  N F  L  L  L L  NK+  IS+ + + L+ +K L + +N   C+CHL WL  +L     I   GA    CS P RL++  IS +   +F+C  SED ++ L   C  D  VC ++C C    +V C   +L++IP  LP +  +L L DN I+ L   G   K   L+KI L+ N + DI  G F G   +  + L  N L+ ++   F GL  L  L L+ N I C+   T      + LL L +N+++++    F    +L  + L+ NP  CDC ++W+  ++K     +    C +P  L+  P++++   +F C+          +  C+P V  CP  CTCS  VV CSN GL SLP+ I   T  L +  N L  +P    +     L  +DLS N I +L P +F+  ++L  + L+ N+++C+    F  L+ L +L L  N +S I   +F +  +L +L L  N  +CDC+L+W+S+W +  +   G  R   C  P  + D  +       F C G
Sbjct:  274 APQASVCPASCSCTNNIVDCRRKGLTEIPANLPEGIVEIRLEQNLIKSIPPGAFSPYKKLKRIDLSKNHISDIADDAFSGLRSLTSLVLYGNKIAEIPKGLFDGLVSLQLLLLNANKINCLRVNTFKDLQSLNLLSLYDNKLQTISKGLFTPLRAIKTLHLAQNPFMCDCHLKWLADYLFDNP-IETSGAR---CSHPRRLANKRISQIKGKKFRCTGSEDYRSRLTGECFQD-LVCPERCRC-EGTVVDCSNLKLTSIPPHLPEHTTDLRLNDNEISSLDATGAFKKLPNLRKINLSNNKLRDIREGVFDGAGGVLELLLTGNKLQAVQGRMFRGLTGLKTLMLRSNQITCVDNATFTGLSSVRLLSLYDNRISTIAPGAFNTLHSLSTINLLSNPYVCDCHLAWLGLWLKKTRVVSGNPRCQRPAFLKEIPIQDVAAPDFTCDG-------VEENACLPSVPRCPDPCTCSDTVVRCSNHGLRSLPKGIPKDTTELYLEGNMLTSVP--KELSSLKQLSLVDLSNNSISSLTPFIFSNMTQLSTLILSYNQIRCVPVHAFDGLRSLRLLTLHGNDLSTIPEGAFNHLTSLSHLALGANPLYCDCELRWLSQWVKAGFKEPGIAR---CTGPPDMADRLLLTTPLNRFQCKG 908          
BLAST of slit1 vs. Ensembl Cavefish
Match: slit3 (slit guidance ligand 3 [Source:NCBI gene;Acc:103037708])

HSP 1 Score: 683.715 bits (1763), Expect = 0.000e+0
Identity = 484/1553 (31.17%), Postives = 743/1553 (47.84%), Query Frame = 1
Query:  157 CPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQLQCINDNIFNKLGMLKYLLLEGNKIFWIS-EAISKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCASEDKANLENMCPSDKKVCSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQIYNFQC--NKEFESKYETHDEECVPKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLPPIYSIHEAPNLMKLDLSFNQIETLEPDVFTRS------------------------SKLREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQFHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCDG---NA---SVYHSVDSCHETNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVLSFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICK-NIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYC-NDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNSIYF-QKNSYIEISPPSRGFLMPRGNISLNFMTNSSSGLILYQGSEQTYLVVNLYQGHLRISYNLGDKVVGPEGYSMGMVNDSRLHALHIEIVGENLTMYLNGRVHCTLKSKYLNSNRYMALDTGIFIGGAPHKILQSAIMKFHIETDSEFIGCMESLLINRKKIDFSKYLPKSINVHPGCGKLISTV---------VASDLKIPSIMXXXXXXXXXXXXXXXNNVCKRPGICKNQGVCEVLPGSKKKFRCICKNGFVGRKCKKRKS--MCHGTFTESH--IVDPDSQGTCKSIERFQYRICTG--TCSTSRMQSDVIKNFYHK-------------PIDPSSSRCCQPTKYSTHEIQFKCENGRVYRRAANLPKNCKC 4623
            CP  C C    VEC+ + F +VP  +     RL++  N I  I K DF  L NL+VL L NN+I+ IE GAF +L  L +LRLNRN +Q LP+ LF    KL +LDL +NQ+Q I    F  +  +K L L  N I  I   + + +  L+ LR+H N L+C+CHL+WL+ +L+Q+  +    AH   C +P  +  + +  +   +F C    ++   + C     VC   C+C+++ IV C  K L+ IP +LP  +VE+ L+ N I  +  G  S YK LK+I L++N + DI   AF GL  LTS+ L  N +  I K  F GLVSL  L L  N I CL  +T +  + L+LL L +NKL ++ K  F P   ++ L+L  NP  CDC + W+ DY+ D    T  A C  PR L    +  ++   F+C  ++++ S+      EC   + CP +C C   VV+CSN  L S+P  +   T  L ++ N +  L    +  + PNL K++LS N++  +   VF  +                        + L+ + L SN++ C++N TF  L  + +L L +N IS I   +F    +L  + L  N + CDC L W+  W +    V GN   P CQ P  LK+ PI  +    F CDG   NA   SV    D C  +++V                           V CS+  L+ +P  IPKDTTELYL+ N L S+ +    +L  L  + LS N I+ L   + + +  L TL+LS+N+++C+   AF  L +L++L L  ND+STIP  AFN L +L+++ALG NP +CDC ++WL+Q+    F + GI+ C  P  M  + +  +    F C     D  + +KC  C+  PC++NG C      +Y C CP+ + G+NC+   NAC  QPC NGG C       G++ CVCP GF G +C +N +DC  N C N +TC D  N+Y+C C   +RG  C+     C    +PCQ+  +C  L    Y+C C  G+ G+ CE++ +DC  N C+H ++CVD  N Y C+C+ G+ G FCE P   P +L                      +   S C   +C N   C+  +     +  C+C PG+ G+ CQ +++++F  K S+I++    R  ++   +ISL   T+  +G++LY+  +   L + LYQGH+R+ Y++  K      YS+  VND   H + + I  + L++ ++     +L    L     +  +T ++IGG P ++   +           F GC+ ++ IN +  +    L     + PGC                V    + P+           T+    N+ C   G C   G+          + C C  G+ G  C  R+    C G         VD   + TC     +    C    +C    ++  + ++  HK             P       CC  TK    +I FKC +G  +        +C C
Sbjct:   33 CPAKCSCSGSHVECQQQGFRSVPRGIPRNAERLDVDRNNITRITKVDFSGLKNLRVLHLENNQISVIERGAFQDLKQLERLRLNRNRLQVLPELLFQSTPKLGRLDLSENQIQAIPRKSFRGITNVKNLTLNNNNITLIPLSSFNHMPKLRTLRLHSNNLHCDCHLSWLSDWLRQRKGL----AHFTQCMSPAHMRGLSVPDVQKRDFVCTGPAQSEPRS-CAPQASVCPASCSCTNN-IVDCRRKGLTEIPANLPEGIVEIRLEQNLIKSIPPGAFSPYKKLKRIDLSKNHISDIADDAFSGLRSLTSLVLYGNKIAEIPKGLFDGLVSLQLLLLNANKINCLRVNTFKDLQSLNLLSLYDNKLQTISKGLFTPLRAIKTLHLAQNPFMCDCHLKWLADYLFDNPIETSGARCSHPRRLANKRISQIKGKKFRCTGSEDYRSRLTG---ECFQDLVCPERCRCEGTVVDCSNLKLTSIPPHLPEHTTDLRLNDNEISSLDATGAFKKLPNLRKINLSNNKLRDIREGVFDGAGGVLELLLTGNKLQAVQGRMFRGLTGLKTLMLRSNQITCVDNATFTGLSSVRLLSLYDNRISTIAPGAFNTLHSLSTINLLSNPYVCDCHLAWLGLWLKKTRVVSGN---PRCQRPAFLKEIPIQDVAAPDFTCDGVEENACLPSVPRCPDPCTCSDTV---------------------------VRCSNHGLRSLPKGIPKDTTELYLEGNMLTSVPK-ELSSLKQLSLVDLSNNSISSLTPFIFSNMTQLSTLILSYNQIRCVPVHAFDGLRSLRLLTLHGNDLSTIPEGAFNHLTSLSHLALGANPLYCDCELRWLSQWVKAGFKEPGIARCTGPPDMADRLLLTTPLNRFQCKGPV-DLNLMSKCAPCLATPCQNNGTCVSDVTGSYHCTCPFGYKGRNCEIAANACLSQPCINGGTCHLTPGQEGHFSCVCPPGFEGQRCEVNPNDCEDNDCENNSTCVDGINNYTCVCPPNYRGDLCEEVVDACLPGFDPCQHDSKCVPL-TRGYRCECLPGYVGQHCEQDYNDCLENKCQHGAECVDAINGYTCVCKDGFSGLFCENPP--PMIL----------------------LQKTSPCDQSDCQNGAECVLME----GEPVCRCLPGFFGNKCQKMHTVHFLGKESFIQL---PRTKILSSTHISLQVATDKDNGMLLYK-EDHDPLAIELYQGHIRLMYDIA-KYPPTTVYSVESVNDGFFHTVELLIQNQTLSLVVDKGAPKSLGK--LPRPPTVETNTRLYIGGVPSELSSPSSRSGADRQSQSFNGCIHNVRINGELQELGSGLDSVKAILPGCHACSVCAQGACRQRDDVGVTCECPAGSTGPLCDQQGTSNPCQNSRCVH-GQCVANGL---------AYSCKCAQGYSGPYCATREEPLACRGLHCVHGECRVDESGEATCACQPGYTGPTCETELSCQGEMIREQLKRHMGHKTCTSTIKVPRMECPRVCHPGLCCTSTKSRRRKITFKCSDGFTFSEELETTLDCNC 1498          

HSP 2 Score: 283.108 bits (723), Expect = 7.919e-77
Identity = 207/653 (31.70%), Postives = 317/653 (48.55%), Query Frame = 1
Query:  139 SDESEICPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQLQCINDNIFNKLGMLKYLLLEGNKIFWISEAI-SKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQC-ASED-KANLENMCPSDKKVCSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQ-GYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQIYNFQCNKEFESKYETHDEECVPKVS-CPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLPPIYSIHEAPNLMKLDLSFNQIETLEPDVFTRSSKLREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQFHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCDG 2082
            + ++ +CP  C C N  V+C+ +    +P  L E    + L  N I  I    F     LK + LS N I+ I   AF  L +L  L L  N I  +P  LF  L  LQ L L  N++ C+  N F  L  L  L L  NK+  IS+ + + L+ +K L + +N   C+CHL WL  +L     I   GA    CS P RL++  IS +   +F+C  SED ++ L   C  D  VC ++C C    +V C   +L++IP  LP +  +L L DN I+ L   G   K   L+KI L+ N + DI  G F G   +  + L  N L+ ++   F GL  L  L L+ N I C+   T      + LL L +N+++++    F    +L  + L+ NP  CDC ++W+  ++K     +    C +P  L+  P++++   +F C+          +  C+P V  CP  CTCS  VV CSN GL SLP+ I   T  L +  N L  +P    +     L  +DLS N I +L P +F+  ++L  + L+ N+++C+    F  L+ L +L L  N +S I   +F +  +L +L L  N  +CDC+L+W+S+W +  +   G  R   C  P  + D  +       F C G
Sbjct:  250 APQASVCPASCSCTNNIVDCRRKGLTEIPANLPEGIVEIRLEQNLIKSIPPGAFSPYKKLKRIDLSKNHISDIADDAFSGLRSLTSLVLYGNKIAEIPKGLFDGLVSLQLLLLNANKINCLRVNTFKDLQSLNLLSLYDNKLQTISKGLFTPLRAIKTLHLAQNPFMCDCHLKWLADYLFDNP-IETSGAR---CSHPRRLANKRISQIKGKKFRCTGSEDYRSRLTGECFQD-LVCPERCRC-EGTVVDCSNLKLTSIPPHLPEHTTDLRLNDNEISSLDATGAFKKLPNLRKINLSNNKLRDIREGVFDGAGGVLELLLTGNKLQAVQGRMFRGLTGLKTLMLRSNQITCVDNATFTGLSSVRLLSLYDNRISTIAPGAFNTLHSLSTINLLSNPYVCDCHLAWLGLWLKKTRVVSGNPRCQRPAFLKEIPIQDVAAPDFTCDG-------VEENACLPSVPRCPDPCTCSDTVVRCSNHGLRSLPKGIPKDTTELYLEGNMLTSVP--KELSSLKQLSLVDLSNNSISSLTPFIFSNMTQLSTLILSYNQIRCVPVHAFDGLRSLRLLTLHGNDLSTIPEGAFNHLTSLSHLALGANPLYCDCELRWLSQWVKAGFKEPGIAR---CTGPPDMADRLLLTTPLNRFQCKG 884          
BLAST of slit1 vs. Ensembl Cavefish
Match: slit3 (slit guidance ligand 3 [Source:NCBI gene;Acc:103037708])

HSP 1 Score: 677.552 bits (1747), Expect = 0.000e+0
Identity = 471/1553 (30.33%), Postives = 734/1553 (47.26%), Query Frame = 1
Query:  157 CPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQLQCINDNIFNKLGMLKYLLLEGNKIFWIS-EAISKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCASEDKANLENMCPSDKKVCSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQIYNFQC--NKEFESKYETHDEECVPKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLPPIYSIHEAPNLMKLDLSFNQIETLEPDVFTRS------------------------SKLREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQFHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCDG---NA---SVYHSVDSCHETNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVLSFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICK-NIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYC-NDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNSIYF-QKNSYIEISPPSRGFLMPRGNISLNFMTNSSSGLILYQGSEQTYLVVNLYQGHLRISYNLGDKVVGPEGYSMGMVNDSRLHALHIEIVGENLTMYLNGRVHCTLKSKYLNSNRYMALDTGIFIGGAPHKILQSAIMKFHIETDSEFIGCMESLLINRKKIDFSKYLPKSINVHPGCGKLISTV---------VASDLKIPSIMXXXXXXXXXXXXXXXNNVCKRPGICKNQGVCEVLPGSKKKFRCICKNGFVGRKCKKRKS--MCHGTFTESH--IVDPDSQGTCKSIERFQYRICTG--TCSTSRMQSDVIKNFYHK-------------PIDPSSSRCCQPTKYSTHEIQFKCENGRVYRRAANLPKNCKC 4623
            CP  C C    VEC+ + F +VP  +     RL+L  N+I+VI +  F  L  L+ L+L+ NR+  +    F +   L +L L+ N IQ +P   F  +T ++ L L  N + CI D  F  L  L+ L L  N I  I   + + +  L+ LR+H N L+C+CHL+WL+ +L+Q+  +    AH   C +P  +  + +  +   +F C    ++   + C     VC   C+C+++ IV C  K L+ IP +LP  +VE+ L+ N I  +  G  S YK LK+I L++N + DI   AF GL  LTS+ L  N +  I K  F GLVSL  L L  N I CL  +T +  + L+LL L +NKL ++ K  F P   ++ L+L  NP  CDC + W+ DY+ D    T  A C  PR L    +  ++   F+C  ++++ S+      EC   + CP +C C   VV+CSN  L S+P  +   T  L ++ N +  L    +  + PNL K++LS N++  +   VF  +                        + L+ + L SN++ C++N TF  L  + +L L +N IS I   +F    +L  + L  N + CDC L W+  W +    V GN   P CQ P  LK+ PI  +    F CDG   NA   SV    D C  +++V                           V CS+  L+ +P  IPKDTTELYL+ N L S+ +    +L  L  + LS N I+ L   + + +  L TL+LS+N+++C+   AF  L +L++L L  ND+STIP  AFN L +L+++ALG NP +CDC ++WL+Q+    F + GI+ C  P  M  + +  +    F C     D  + +KC  C+  PC++NG C      +Y C CP+ + G+NC+   NAC  QPC NGG C       G++ CVCP GF G +C +N +DC  N C N +TC D  N+Y+C C   +RG  C+     C    +PCQ+  +C  L    Y+C C  G+ G+ CE++ +DC  N C+H ++CVD  N Y C+C+ G+ G FCE P   P +L                      +   S C   +C N   C+  +     +  C+C PG+ G+ CQ +++++F  K S+I++    R  ++   +ISL   T+  +G++LY+  +   L + LYQGH+R+ Y++  K      YS+  VND   H + + I  + L++ ++     +L    L     +  +T ++IGG P ++   +           F GC+ ++ IN +  +    L     + PGC                V    + P+           T+    N+ C   G C   G+          + C C  G+ G  C  R+    C G         VD   + TC     +    C    +C    ++  + ++  HK             P       CC  TK    +I FKC +G  +        +C C
Sbjct:   33 CPAKCSCSGSHVECQQQGFRSVPRGIPRNAERLHLENNQISVIERGAFQDLKQLERLRLNRNRLQVLPELLFQSTPKLGRLDLSENQIQAIPRKSFRGITNVKNLQLDSNHISCIEDGAFRALRDLEILTLNNNNITLIPLSSFNHMPKLRTLRLHSNNLHCDCHLSWLSDWLRQRKGL----AHFTQCMSPAHMRGLSVPDVQKRDFVCTGPAQSEPRS-CAPQASVCPASCSCTNN-IVDCRRKGLTEIPANLPEGIVEIRLEQNLIKSIPPGAFSPYKKLKRIDLSKNHISDIADDAFSGLRSLTSLVLYGNKIAEIPKGLFDGLVSLQLLLLNANKINCLRVNTFKDLQSLNLLSLYDNKLQTISKGLFTPLRAIKTLHLAQNPFMCDCHLKWLADYLFDNPIETSGARCSHPRRLANKRISQIKGKKFRCTGSEDYRSRLTG---ECFQDLVCPERCRCEGTVVDCSNLKLTSIPPHLPEHTTDLRLNDNEISSLDATGAFKKLPNLRKINLSNNKLRDIREGVFDGAGGVLELLLTGNKLQAVQGRMFRGLTGLKTLMLRSNQITCVDNATFTGLSSVRLLSLYDNRISTIAPGAFNTLHSLSTINLLSNPYVCDCHLAWLGLWLKKTRVVSGN---PRCQRPAFLKEIPIQDVAAPDFTCDGVEENACLPSVPRCPDPCTCSDTV---------------------------VRCSNHGLRSLPKGIPKDTTELYLEGNMLTSVPK-ELSSLKQLSLVDLSNNSISSLTPFIFSNMTQLSTLILSYNQIRCVPVHAFDGLRSLRLLTLHGNDLSTIPEGAFNHLTSLSHLALGANPLYCDCELRWLSQWVKAGFKEPGIARCTGPPDMADRLLLTTPLNRFQCKGPV-DLNLMSKCAPCLATPCQNNGTCVSDVTGSYHCTCPFGYKGRNCEIAANACLSQPCINGGTCHLTPGQEGHFSCVCPPGFEGQRCEVNPNDCEDNDCENNSTCVDGINNYTCVCPPNYRGDLCEEVVDACLPGFDPCQHDSKCVPL-TRGYRCECLPGYVGQHCEQDYNDCLENKCQHGAECVDAINGYTCVCKDGFSGLFCENPP--PMIL----------------------LQKTSPCDQSDCQNGAECVLME----GEPVCRCLPGFFGNKCQKMHTVHFLGKESFIQL---PRTKILSSTHISLQVATDKDNGMLLYK-EDHDPLAIELYQGHIRLMYDIA-KYPPTTVYSVESVNDGFFHTVELLIQNQTLSLVVDKGAPKSLGK--LPRPPTVETNTRLYIGGVPSELSSPSSRSGADRQSQSFNGCIHNVRINGELQELGSGLDSVKAILPGCHACSVCAQGACRQRDDVGVTCECPAGSTGPLCDQQGTSNPCQNSRCVH-GQCVANGL---------AYSCKCAQGYSGPYCATREEPLACRGLHCVHGECRVDESGEATCACQPGYTGPTCETELSCQGEMIREQLKRHMGHKTCTSTIKVPRMECPRVCHPGLCCTSTKSRRRKITFKCSDGFTFSEELETTLDCNC 1498          

HSP 2 Score: 282.722 bits (722), Expect = 9.844e-77
Identity = 207/653 (31.70%), Postives = 317/653 (48.55%), Query Frame = 1
Query:  139 SDESEICPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQLQCINDNIFNKLGMLKYLLLEGNKIFWISEAI-SKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQC-ASED-KANLENMCPSDKKVCSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQ-GYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQIYNFQCNKEFESKYETHDEECVPKVS-CPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLPPIYSIHEAPNLMKLDLSFNQIETLEPDVFTRSSKLREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQFHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCDG 2082
            + ++ +CP  C C N  V+C+ +    +P  L E    + L  N I  I    F     LK + LS N I+ I   AF  L +L  L L  N I  +P  LF  L  LQ L L  N++ C+  N F  L  L  L L  NK+  IS+ + + L+ +K L + +N   C+CHL WL  +L     I   GA    CS P RL++  IS +   +F+C  SED ++ L   C  D  VC ++C C    +V C   +L++IP  LP +  +L L DN I+ L   G   K   L+KI L+ N + DI  G F G   +  + L  N L+ ++   F GL  L  L L+ N I C+   T      + LL L +N+++++    F    +L  + L+ NP  CDC ++W+  ++K     +    C +P  L+  P++++   +F C+          +  C+P V  CP  CTCS  VV CSN GL SLP+ I   T  L +  N L  +P    +     L  +DLS N I +L P +F+  ++L  + L+ N+++C+    F  L+ L +L L  N +S I   +F +  +L +L L  N  +CDC+L+W+S+W +  +   G  R   C  P  + D  +       F C G
Sbjct:  250 APQASVCPASCSCTNNIVDCRRKGLTEIPANLPEGIVEIRLEQNLIKSIPPGAFSPYKKLKRIDLSKNHISDIADDAFSGLRSLTSLVLYGNKIAEIPKGLFDGLVSLQLLLLNANKINCLRVNTFKDLQSLNLLSLYDNKLQTISKGLFTPLRAIKTLHLAQNPFMCDCHLKWLADYLFDNP-IETSGAR---CSHPRRLANKRISQIKGKKFRCTGSEDYRSRLTGECFQD-LVCPERCRC-EGTVVDCSNLKLTSIPPHLPEHTTDLRLNDNEISSLDATGAFKKLPNLRKINLSNNKLRDIREGVFDGAGGVLELLLTGNKLQAVQGRMFRGLTGLKTLMLRSNQITCVDNATFTGLSSVRLLSLYDNRISTIAPGAFNTLHSLSTINLLSNPYVCDCHLAWLGLWLKKTRVVSGNPRCQRPAFLKEIPIQDVAAPDFTCDG-------VEENACLPSVPRCPDPCTCSDTVVRCSNHGLRSLPKGIPKDTTELYLEGNMLTSVP--KELSSLKQLSLVDLSNNSISSLTPFIFSNMTQLSTLILSYNQIRCVPVHAFDGLRSLRLLTLHGNDLSTIPEGAFNHLTSLSHLALGANPLYCDCELRWLSQWVKAGFKEPGIAR---CTGPPDMADRLLLTTPLNRFQCKG 884          
BLAST of slit1 vs. Ensembl Cavefish
Match: slit1a (slit homolog 1a (Drosophila) [Source:ZFIN;Acc:ZDB-GENE-030722-3])

HSP 1 Score: 633.254 bits (1632), Expect = 0.000e+0
Identity = 450/1571 (28.64%), Postives = 715/1571 (45.51%), Query Frame = 1
Query:  148 SEICPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQLQCINDNIFNKLGMLKYLLLEGNKIFWIS-EAISKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCASEDKANLENMCPSDKKVCSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQIYNFQCNKEFESKYETHDEECVPKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLPPIYSIHEAPNLMKLDLSFNQIETLEPDVFTRSSKLREISLNSNRLKCINNETFKNLKEL---------------------------------------------EILQLSENAISCIVTDSFXXXXXXXXXXXXXXQFHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCDGNASVYHSVDSCHETNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVLSFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYC-DIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNSIYF-QKNSYIEISPPSRGFLMPRGNISLNFMTNSSSGLILYQGSEQTYLVVNLYQGHLRISYNLGDKVVGPEGYSMGMVNDSRLHALHIEIVGENLTMYLNGRVHCTLKSKYLNSNRYMALDTGIFIGGAPHKILQSAIMKFHIETDSEFIGCMESLLINRKKIDFSKYLPKSINVHPGCGKLISTVVASDLKIPSIMXXXXXXXXXXXXXXXNNVCKRPGICKNQGV----CEVLPGSKKKFRCICKNGFVGRKCKK--------RKSMC-HGTFTESHIVDP-------------DSQGTCKS---IERFQYRICTGTCSTSRMQSDVIKNFYHKPIDPSSSRCCQPTKYSTHEIQFKCENGRVYRRAANLPKNCKCVA 4629
            S  CP  C C   +V+            L++      L+ N+I  I +  F  +  L+ L+L+ N++  +    F     L +L L+ N IQ +P   F   T ++ L L  N + CI D  F  L  L+ L L  N I  I   + + +  L+  R+H N LNC+CHL+WL+ +L+Q+  +         CS+P +L  I ++ +  +EF C+   + +    C      C   C CS++ IV C GK L+ IP +LP N+ E+ L+ N I  +  G  S YK L++I L+ N + +I P AF GL  L S+ L  N +  + K  F GL +L  L L  N I C+  +T +  + L LL L +NK+ +L K TF     ++ L+L  NP  CDC + W+ DY++     T  A C  PR L    +  ++   F+C+   +++    + EC     CP KC C S+VV+CSN  L  +PE I  ST  L +++N +  L    +      L K++LS N+I  +E  VF  +S + E+ L +N+L  + +  FK L+ L                                              +L L +N ++ I   +F     L  L L  N F+CDC+L W+ +W R+   V GN   P CQ P  LK+ P      +      +AS         E   ++T                         V CS+  L+ +P  IP++ TELYLD N   S+          L+ + LS N I  L       +  L TL+LS+N L+CI   AF  L +L++L L  ND+S +P   F+D+ +L+++A+G NP +CDC ++WL+ +    + + GI+ C  P  M+ K +  +    F C    + A I AKC+ C+  PC ++G C       Y+C C   F GK+C+  +NAC   PC NGG+C+       + C C +GF GP C IN+DDC  N C NGATC D  N+Y+C C   + G+ C +++ +     +PC++   C L+     KCVC+ G+ G  C  + DDC+ + C++  +CVD  N Y C C  GY G+ CE+P                                 S+C   +C N+  C+     +  ++ C+C PG+ G  C+ L S+ F  ++SY+ +S        P+ NI+L   T   +G++LY G +  ++ V LYQGH+++SY+ G +  G   YS   +ND + H + +    + + + ++G +  T+ S      R +  +  +++GG P  +  SA+  + I+  S F GC+++L IN +  DF+K   K   V PGC           +  P                     C +P     QG      + +P   + +RC C+ G+ G  C +        R+  C HG    S   D              D++  C+     + +Q +     C T+RM S     +        +  CC   +    +  F+C +G  +         C CV 
Sbjct:   38 SSACPALCTCSGTTVDLNLSFL----AFLEQ------LMENQITAIERGAFDDMKELERLRLNRNQLHQLPELLFQKNAALSRLDLSENFIQAIPRKAFRGATDIKNLQLDKNHIGCIEDGAFRALRGLEVLTLNNNNISGIPVSSFNHMPKLRTFRLHSNSLNCDCHLSWLSQWLRQRPTL----GLFTQCSSPAQLRGINVAEIQKHEFSCSGHAENSNPPSCNLAAGSCPAMCTCSNN-IVDCRGKGLTAIPANLPDNMAEIRLEQNGIKSVPPGAFSPYKKLRRIDLSNNQISEIAPDAFQGLRSLNSLVLYGNKITELPKGVFDGLYALQLLLLNANKIHCVRANTFQDLQNLSLLSLYDNKIQTLAKGTFASLRAIQTLHLAQNPFICDCNLKWLADYLRSNPIETSGARCASPRRLANKRIGQIKSKKFRCSGTEDTRL---NNECNNDPVCPAKCRCESNVVDCSNLRLTKIPEHIPASTTELRLNNNEITSLEATGAFKTLTQLKKINLSNNKISEIEDGVFEGASSVNELHLTANQLDSVRSSMFKGLEGLRMLNQFSGQELTKILHYVFFLSPECSATTASAASTTAASLGLYNVRLLSLYDNQLTTITPGAFDTLQTLSTLNLLANSFNCDCRLAWLGDWLRSRKIVTGN---PRCQRPAFLKEIPAAGRGQE------DASCMPRPQCPSECTCLET------------------------VVRCSNKHLRALPKGIPRNVTELYLDGNQF-SMVPKELAAFKYLQLVDLSNNKINSLTNASFANMSQLTTLILSYNSLRCIPSLAFSGLRSLRLLSLHGNDISELPDGIFSDVMSLSHLAIGANPLYCDCRLRWLSDWVKTGYKEPGIARCTGPPGMEGKLLLTTPAKKFECQGNVDPA-ILAKCNPCLSSPCLNHGSCHTDPVEVYKCTCAEGFKGKHCETALNACVSNPCANGGVCQVTGEGDAFSCSCSLGFEGPSCEINIDDCEDNDCENGATCIDGVNNYTCLCSPYYTGEMCEEMEDMCAPGHSPCKHQSTC-LITATGPKCVCAPGYVGDDCSVDYDDCQEHKCQNNGECVDEINGYSCSCTRGYSGQLCEVPP-----------------------------APRSLCELADCQNDAPCV----ERAGRALCQCLPGFGGPRCEKLVSVNFVDRDSYLLLSDLKN---WPQANITLQVSTAEDNGILLYNG-DNDHIAVELYQGHVKVSYDPGSQ-PGHAIYSTETINDGQFHTVELVTFDQMVNLSIDGGLPTTMDS--FGKLRPLNGEAPLYVGGMPLDVPSSALRMWQIQNGSSFHGCIQNLYINNELQDFTKTQMKP-GVVPGCEPCRKIYCLHGICQPDAAAGPVCHCQPGWSGPH---CDQPATSPCQGSKCVHGKCVPLDTQTYRCECQEGYRGALCNQQGELFNPCRRLQCKHGRCQVSDTGDAYCHCESGYTGDLCDAESECRGEPVRDFYQVQRGYAICQTTRMVS-----WVECSGACDTGACCASQRMKRRKYTFECSDGTSFSEEVEKTIKCGCVG 1505          

HSP 2 Score: 149.443 bits (376), Expect = 1.205e-35
Identity = 126/467 (26.98%), Postives = 208/467 (44.54%), Query Frame = 1
Query:  127 INDPSDESEICPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLK-LNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLD-LRDNQLQCINDNIF------------------NKLGM--LKYLLLEGNKIFWISE-AISKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCASEDKANLENMCPSDKKVCSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQIYNFQC 1458
            +N+  +   +CP  C+CE+  V+C +     +P  +   TT L L  N I  +  +   K L  LK + LSNN+I+ IE G F+   ++ +L L  N +  +   +F  L  L+ L+     +L  I   +F                    LG+  ++ L L  N++  I+  A   L+ L  L +  N  NC+C LAWL  +L+ +  +      +  C  P  L  IP +     +  C    +      CPS+       C C  + +V+C  K L  +P+ +P NV EL L  N  +++ +  L+ +K+L+ + L+ N +  +   +F  ++QLT++ L+ N+L+ I   AF GL SL  L L  NDI  LP                           F   ++L +L +  NPL CDCR+ W+ D++K       +A C  P  ++G  L       F+C
Sbjct:  468 LNNECNNDPVCPAKCRCESNVVDCSNLRLTKIPEHIPASTTELRLNNNEITSLEATGAFKTLTQLKKINLSNNKISEIEDGVFEGASSVNELHLTANQLDSVRSSMFKGLEGLRMLNQFSGQELTKILHYVFFLSPECSATTASAASTTAASLGLYNVRLLSLYDNQLTTITPGAFDTLQTLSTLNLLANSFNCDCRLAWLGDWLRSRKIVTG----NPRCQRPAFLKEIPAAGRGQEDASCMPRPQ------CPSE-------CTC-LETVVRCSNKHLRALPKGIPRNVTELYLDGNQFSMVPK-ELAAFKYLQLVDLSNNKINSLTNASFANMSQLTTLILSYNSLRCIPSLAFSGLRSLRLLSLHGNDISELPDG------------------------IFSDVMSLSHLAIGANPLYCDCRLRWLSDWVKTGYKEPGIARCTGPPGMEGKLLLTTPAKKFEC 891          
BLAST of slit1 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000003392.1 (pep scaffold:Pmarinus_7.0:GL476560:299294:319002:1 gene:ENSPMAG00000003104.1 transcript:ENSPMAT00000003392.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 217.624 bits (553), Expect = 1.629e-59
Identity = 183/725 (25.24%), Postives = 288/725 (39.72%), Query Frame = 1
Query: 2281 YLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVLSFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATC----NDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSG--------QCQLLPNNDYKCV-CSKGWE--------------GKQCERNTDD----CKYNSCKH-----ESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNSIYF-QKNSYIEISPPSRGFLMPRGNISLNFMTNSSSGLILYQGSEQTYLVVNLYQGHLRISYNLGDKVVGPEGYSMGMVNDSRLHALHIEIVGENLTMYLNGRVHCTLKSKYLNSNRYMALDTGIFIGGAPHKILQSAIMKFHIETDSEFIGCMESLLINRKKIDFSKYLPKSINVHPGCGKLISTVVASDLKIPSIMXXXXXXXXXXXXXXXNNVCKRPGICKNQGVCEVLPGSKKKFRCICKNGFVGRKCKKRKS 4344
            YLD N L ++      N  +L  + LS N I+ +     + +  L TL+LS+N+L+C+   AF  L  L++L L  NDVS IP+  F+DL +L+++ALG NP HCDC ++WL+ +    + + GI+ C+ P  M  K +  +    F C     D  I AKC                                       N C  QPC N G+C + D    Y C C  GF+G  C   ++ CV + C N  TC    +  ++ +    +   R   C I  L C +    +             QLL       V     W               G Q + ++D     C + SC+       +    L     C C     G  CE+P                    M Y       +  S C YH+C N G C+        +  C+C  G+ G  C  L S+ F Q++SY+++ PP +G   P+ NISL   T+  +G++LY G  + ++ V +Y+G +R+SY+ G     P  YS+  +ND   H + I  + + + + ++G    T+    L     + L+  ++IGG P  +  +A+ + H      F GC+  L IN +  D          + PGC                                    C++ G C + G C           C C+ G+ G +C +R  
Sbjct:    1 YLDGNQL-TMVPPELSNYKHLSFIDLSNNHISTIANFTFSNITQLSTLILSYNRLRCLPLRAFAGLKTLRLLSLHGNDVSMIPHGVFSDLTSLSHLALGSNPLHCDCAMRWLSDWVKVDYKEPGIARCSGPGDMAEKLLLTTPSKKFECVGP-PDVSIQAKC---------------------------------------NPCLSQPCLNHGVCSS-DPVDFYRCTCSYGFKGQDCGTPINACVSSPCQNAGTCHVREDGDDSGFQLYRRPDVRAGNCQINVLNCPNFLDFKRKTVEFNSTVVMAQLLTTTKTSQVHLHHSWHSPPPHPFSLPTRFGGPQSDWSSDSYCAICYFLSCESIVYPTGTDVTSLRTTNTCPCPTPGSGPLCEVPP------------------PMVY-------LQTSACQYHDCQNGGQCVE----AGGEPECQCLQGFVGSRCDKLTSVNFVQRDSYLQL-PPIKG--QPKANISLRVATDEDNGMLLYNGVSE-HIAVEIYRGRVRLSYDAG-SYPNPTIYSLETINDGEFHTVEIVTLEQMVNLSIDGGSPKTMIK--LGKPASLTLELPLYIGGMPTDVNSAALKQMHDRNGMGFHGCIRDLYINDELQDLRAPGRPPAGILPGCAP----------------------------------CRQQGACVH-GACRA--AGPSGHTCECEAGWTGAQCDQRSG 610          

HSP 2 Score: 76.6406 bits (187), Expect = 3.069e-14
Identity = 48/163 (29.45%), Postives = 72/163 (44.17%), Query Frame = 1
Query: 1069 QLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQIYNFQCNKEFESKYETHDEECVPKVSCPLKCTCSSDVVN 1557
             L+ I L+ N++ TI    F  +  L  L L YN + CLP       K L LL L  N ++ +    F    +L +L L  NPL+CDC M W+ D++K       +A C  P ++    L       F+C    +   +     C+ +  C     CSSD V+
Sbjct:   19 HLSFIDLSNNHISTIANFTFSNITQLSTLILSYNRLRCLPLRAFAGLKTLRLLSLHGNDVSMIPHGVFSDLTSLSHLALGSNPLHCDCAMRWLSDWVKVDYKEPGIARCSGPGDMAEKLLLTTPSKKFECVGPPDVSIQAKCNPCLSQ-PCLNHGVCSSDPVD 180          

HSP 3 Score: 72.0182 bits (175), Expect = 8.448e-13
Identity = 50/158 (31.65%), Postives = 77/158 (48.73%), Query Frame = 1
Query: 1621 MSHNNLKKLPPIYSIHEAPNLMKLDLSFNQIETLEPDVFTRSSKLREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQFHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCDGNASV 2094
            +  N L  +PP  S ++  +L  +DLS N I T+    F+  ++L  + L+ NRL+C+    F  LK L +L L  N +S I    F +  +L +L L  N  HCDC ++W+S+W +  Y   G  R   C  P  + +  +     K F C G   V
Sbjct:    2 LDGNQLTMVPPELSNYK--HLSFIDLSNNHISTIANFTFSNITQLSTLILSYNRLRCLPLRAFAGLKTLRLLSLHGNDVSMIPHGVFSDLTSLSHLALGSNPLHCDCAMRWLSDWVKVDYKEPGIAR---CSGPGDMAEKLLLTTPSKKFECVGPPDV 154          

HSP 4 Score: 61.6178 bits (148), Expect = 1.375e-9
Identity = 52/207 (25.12%), Postives = 90/207 (43.48%), Query Frame = 1
Query:  259 LLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQLQCINDNIFNKLGMLKYLLLEGNKIFWISEAI-SKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCASEDKANLENMC-PSDKKVCSKKCNCSHDGI 873
            L GN++ ++   +     +L  + LSNN I++I                         +  FS +T+L  L L  N+L+C+    F  L  L+ L L GN +  I   + S L  L  L +  N L+C+C + WL+ ++  K +  +PG     CS P  ++   + +  S +F+C      +++  C P   + C     CS D +
Sbjct:    2 LDGNQLTMV-PPELSNYKHLSFIDLSNNHISTIA------------------------NFTFSNITQLSTLILSYNRLRCLPLRAFAGLKTLRLLSLHGNDVSMIPHGVFSDLTSLSHLALGSNPLHCDCAMRWLSDWV--KVDYKEPGI--ARCSGPGDMAEKLLLTTPSKKFECVGPPDVSIQAKCNPCLSQPCLNHGVCSSDPV 179          

HSP 5 Score: 55.0694 bits (131), Expect = 1.401e-7
Identity = 39/119 (32.77%), Postives = 52/119 (43.70%), Query Frame = 1
Query: 2902 PCRNGGICKN----IDNYGNYECVCPVGFRGPKCTINVDD-CVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQN----SGQCQLLPNNDYKCVCSKGWEGKQCER 3231
            PCR  G C +          + C C  G+ G +C     D C  N CV+GA       SYSC+C +GF G  CD +       NPC+      G C++       C CS G+ G  C+R
Sbjct:  572 PCRQQGACVHGACRAAGPSGHTCECEAGWTGAQCDQRSGDPCQANKCVHGACIALDAFSYSCRCANGFVGTLCDEEQR--ELFNPCRGVRCKHGTCRISGAGKPFCECSPGYTGDNCDR 688          

HSP 6 Score: 51.6026 bits (122), Expect = 1.611e-6
Identity = 33/114 (28.95%), Postives = 52/114 (45.61%), Query Frame = 1
Query: 3025 CVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNS----GQCQLLPNNDYKCVCSKGWEGKQCERNTDD----CKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCE 3342
            CV+GA      + ++C+C++G+ G  CD +       +PCQ +    G C  L    Y C C+ G+ G  C+    +    C+   CKH +  +       C C PGY G  C+
Sbjct:  579 CVHGACRAAGPSGHTCECEAGWTGAQCDQR-----SGDPCQANKCVHGACIALDAFSYSCRCANGFVGTLCDEEQRELFNPCRGVRCKHGTCRISGAGKPFCECSPGYTGDNCD 687          

HSP 7 Score: 49.2914 bits (116), Expect = 8.530e-6
Identity = 38/138 (27.54%), Postives = 55/138 (39.86%), Query Frame = 1
Query: 3154 GQCQLLPNNDYKCVCSKGWEGKQC-ERNTDDCKYNSCKHESQCVDLHN-DYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLC 3561
            G C+    + + C C  GW G QC +R+ D C+ N C H + C+ L    Y C C  G++G  C                 D  Q E+            + C    C +    I+G      K +C+C PGY+G  C
Sbjct:  582 GACRAAGPSGHTCECEAGWTGAQCDQRSGDPCQANKCVHGA-CIALDAFSYSCRCANGFVGTLC-----------------DEEQRELF-----------NPCRGVRCKHGTCRISG----AGKPFCECSPGYTGDNC 686          
BLAST of slit1 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000002643.1 (pep scaffold:Pmarinus_7.0:GL478752:33855:40482:1 gene:ENSPMAG00000002413.1 transcript:ENSPMAT00000002643.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 130.183 bits (326), Expect = 4.952e-33
Identity = 78/247 (31.58%), Postives = 115/247 (46.56%), Query Frame = 1
Query: 2773 CMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMC-RPGYIGKFCEIP----AWKPTVLKTLISRRDINQSEMTYENLENEVISESV--CAYHECLNNGICI 3492
            C   PC H G C       + C C   + G NC+  +  C   PC N  +C  +D  G Y+C C  GF+G  C I++++C    C++G TC +  + YSC C  GF G+ C +    C    PC N   C  L +  ++CVC +G+ G  CE   D+C+   C++   C+DL + Y C C   GY GK CE+     A  P    +      +N + + +     +   E V  C    C+N G CI
Sbjct:    1 CASGPCLHGGTCLPAADGGFSCRCAPGYSGDNCEASVGGCASGPCLNDAVC--VDEPGGYKCYCVPGFQGRHCEIDINECASEPCLHGGTCTNGRDRYSCACPDGFAGERCQLDTNECAS-QPCHNGATCVDL-DGKFECVCPRGYTGAMCETEVDECESGPCQNGGVCIDLVDSYTCDCLETGYEGKDCELEILECASNPCRHNSTCIEGILNFTCICWPGFAGDACEEDVNECGDEPCMNGGQCI 243          

HSP 2 Score: 129.798 bits (325), Expect = 6.161e-33
Identity = 88/264 (33.33%), Postives = 131/264 (49.62%), Query Frame = 1
Query: 2773 CMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKC-QSGFRGKYCDIKFLYCNDINPCQNSGQC--QLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSY-CKCKPGYSG 3552
            C   PC H G C+      Y C CP  F G+ C    N C  QPC NG  C ++D  G +ECVCP G+ G  C   VD+C    C NG  C DL +SY+C C ++G+ GK C+++ L C   NPC+++  C   +L   ++ C+C  G+ G  CE + ++C    C +  QC++L        +P + GK  E+P        +  + R +  +       EN  ++   C    CLN G CI+     N  S+ C+C PG++G
Sbjct:   78 CASEPCLHGGTCT-NGRDRYSCACPDGFAGERCQLDTNECASQPCHNGATCVDLD--GKFECVCPRGYTGAMCETEVDECESGPCQNGGVCIDLVDSYTCDCLETGYEGKDCELEILECAS-NPCRHNSTCIEGIL---NFTCICWPGFAGDACEEDVNECGDEPCMNGGQCIELS-------KPDFYGKLSELPGTFHYANASGFTCRCLPGT-----TGENCSVNLDECESQPCLNGGSCID-----NIGSFSCECAPGFTG 317          

HSP 3 Score: 71.633 bits (174), Expect = 2.202e-13
Identity = 58/206 (28.16%), Postives = 89/206 (43.20%), Query Frame = 1
Query: 2722 ICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDC-PYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDI--KFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIG 3330
            +C      A    + D C   PC++ G C  +   +Y CDC    + GK+C+  I  C   PCR+   C  I+   N+ C+C  GF G  C  +V++C    C+NG  C +L+       +  F GK  ++   F Y N                + + C C  G  G+ C  N D+C+   C +   C+D    + C C PG+ G
Sbjct:  137 VCPRGYTGAMCETEVDECESGPCQNGGVCIDLV-DSYTCDCLETGYEGKDCELEILECASNPCRHNSTC--IEGILNFTCICWPGFAGDACEEDVNECGDEPCMNGGQCIELS-------KPDFYGKLSELPGTFHYAN---------------ASGFTCRCLPGTTGENCSVNLDECESQPCLNGGSCIDNIGSFSCECAPGFTG 317          

HSP 4 Score: 64.3142 bits (155), Expect = 6.192e-11
Identity = 42/143 (29.37%), Postives = 58/143 (40.56%), Query Frame = 1
Query: 3136 NPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQ 3564
             PC + G C    +  + C C+ G+ G  CE +   C    C +++ CVD    YKC C PG+ G+ CEI               DIN+                 CA   CL+ G C NG      +  C C  G++G  CQ
Sbjct:    4 GPCLHGGTCLPAADGGFSCRCAPGYSGDNCEASVGGCASGPCLNDAVCVDEPGGYKCYCVPGFQGRHCEI---------------DINE-----------------CASEPCLHGGTCTNG----RDRYSCACPDGFAGERCQ 110          
BLAST of slit1 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000002014.1 (pep scaffold:Pmarinus_7.0:GL482490:2146:12219:-1 gene:ENSPMAG00000001825.1 transcript:ENSPMAT00000002014.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 123.635 bits (309), Expect = 4.894e-32
Identity = 65/195 (33.33%), Postives = 102/195 (52.31%), Query Frame = 1
Query: 2758 AKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCE 3342
            A  D C   PC++ G+C +    +Y CDC   F GK C+  I+ C   PC+NGG C  ++  G++ C C  G+ G  C  ++D+C  + C NG  C +   S++C C +G+ G  CD     C   +PC N G+C+      + C C+ G+ G +CE + ++C  + C +  +C D    + C C PG+ G  CE
Sbjct:   10 ADVDECSPNPCQNGGRC-IDGAASYTCDCAQGFTGKRCETDIDECTSDPCQNGGQC--VNGAGSFTCSCLAGYTGTLCDSDIDECTSDPCQNGGQCVNGAGSFTCSCLAGYTGTLCDSDVNECAS-SPCLNGGRCR-DGVAAFTCECAPGFTGTRCETDVNECASSPCLNGGRCRDGVAAFTCECAPGFTGTRCE 199          

HSP 2 Score: 111.309 bits (277), Expect = 1.018e-27
Identity = 69/228 (30.26%), Postives = 103/228 (45.18%), Query Frame = 1
Query: 2881 INACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQ 3564
            ++ C   PC+NGG C  ID   +Y C C  GF G +C  ++D+C  + C NG  C +   S++C C +G+ G  CD     C   +PCQN GQC +     + C C  G+ G  C+ + ++C  + C +  +C D    + C C PG+ G  CE                D+N+                 CA   CLN G C +G         C+C PG++G  C+
Sbjct:   12 VDECSPNPCQNGGRC--IDGAASYTCDCAQGFTGKRCETDIDECTSDPCQNGGQCVNGAGSFTCSCLAGYTGTLCDSDIDECTS-DPCQNGGQC-VNGAGSFTCSCLAGYTGTLCDSDVNECASSPCLNGGRCRDGVAAFTCECAPGFTGTRCET---------------DVNE-----------------CASSPCLNGGRCRDG----VAAFTCECAPGFTGTRCE 199          

HSP 3 Score: 94.3597 bits (233), Expect = 6.258e-22
Identity = 51/160 (31.87%), Postives = 83/160 (51.88%), Query Frame = 1
Query: 2767 DSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDC 3246
            D C   PC++ G+C +    ++ C C   + G  CD  I+ C   PC+NGG C  ++  G++ C C  G+ G  C  +V++C  + C+NG  C D   +++C+C  GF G  C+     C   +PC N G+C+      + C C+ G+ G +CE   + C
Sbjct:   51 DECTSDPCQNGGQC-VNGAGSFTCSCLAGYTGTLCDSDIDECTSDPCQNGGQC--VNGAGSFTCSCLAGYTGTLCDSDVNECASSPCLNGGRCRDGVAAFTCECAPGFTGTRCETDVNECAS-SPCLNGGRCRD-GVAAFTCECAPGFTGTRCETVLNPC 205          
BLAST of slit1 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000005361.1 (pep scaffold:Pmarinus_7.0:GL479377:6554:13965:1 gene:ENSPMAG00000004883.1 transcript:ENSPMAT00000005361.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 118.627 bits (296), Expect = 5.069e-30
Identity = 72/196 (36.73%), Postives = 108/196 (55.10%), Query Frame = 1
Query: 2758 AKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEI 3345
            A  + C+  PC++NG C L  ++ + C C   F G  C+  ++ C  +PCR  G C N D  G++ C C +GF GP CT NV+DC  N C++G  C D  N++ C C+ G+ G  CD     C   N C+N   C  L    + C C+ G+ G+ C+ N DDC  N C +++ C+D  N Y C+C  G+ G++C +
Sbjct:    3 ADVNECLSNPCQNNGIC-LNRNNKFLCQCSSGFTGTFCEIDVDECNSRPCRQWGTCVNTD--GSFYCSCVLGFNGPLCTNNVNDCAPNPCIHG-RCVDQVNAFFCSCEEGWSGTTCDTNIDDCVG-NLCRNGATCVDLVK-AFNCSCAAGFTGQYCQNNIDDCIGNQCTNQAICIDGINRYSCICPDGWSGEYCAV 192          

HSP 2 Score: 107.071 bits (266), Expect = 4.255e-26
Identity = 73/205 (35.61%), Postives = 98/205 (47.80%), Query Frame = 1
Query: 2725 CSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKY--NSCKHE-SQCVDLHNDYKCMCRPGYIG 3330
            CS      +     D C  RPC+  G C + T  ++ C C   F+G  C   +N C   PC +G   + +D    + C C  G+ G  C  N+DDCVGNLC NGATC DL  +++C C +GF G+YC      C   N C N   C +   N Y C+C  GW G+ C  + D+C    +SC      C +    Y C C  GY G
Sbjct:   30 CSSGFTGTFCEIDVDECNSRPCRQWGTC-VNTDGSFYCSCVLGFNGPLCTNNVNDCAPNPCIHG---RCVDQVNAFFCSCEEGWSGTTCDTNIDDCVGNLCRNGATCVDLVKAFNCSCAAGFTGQYCQNNIDDCIG-NQCTNQAIC-IDGINRYSCICPDGWSGEYCAVDIDECSTGRSSCHTTLGVCSNSPGSYSCACIAGYEG 228          

HSP 3 Score: 93.2041 bits (230), Expect = 2.567e-21
Identity = 73/231 (31.60%), Postives = 104/231 (45.02%), Query Frame = 1
Query: 2869 CDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLC 3561
            C   +N C   PC+N GIC N +N   + C C  GF G  C I+VD+C    C    TC + + S+ C C  GF G  C      C   NPC + G+C +   N + C C +GW G  C+ N DDC  N C++ + CVDL   + C C  G+ G++C+                          N+++       C  ++C N  ICI+G      +  C C  G+SG  C
Sbjct:    1 CFADVNECLSNPCQNNGICLNRNN--KFLCQCSSGFTGTFCEIDVDECNSRPCRQWGTCVNTDGSFYCSCVLGFNGPLCTNNVNDCAP-NPCIH-GRC-VDQVNAFFCSCEEGWSGTTCDTNIDDCVGNLCRNGATCVDLVKAFNCSCAAGFTGQYCQ-------------------------NNIDD-------CIGNQCTNQAICIDGI----NRYSCICPDGWSGEYC 190          

HSP 4 Score: 67.0106 bits (162), Expect = 2.658e-12
Identity = 55/192 (28.65%), Postives = 83/192 (43.23%), Query Frame = 1
Query: 2989 CTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQ 3564
            C  +V++C+ N C N   C + NN + C+C SGF G +C+I    CN   PC+  G C +  +  + C C  G+ G  C  N +DC  N C H  +CVD  N + C C  G+ G  C+                          N+++       C  + C N   C++  +  N    C C  G++G  CQ
Sbjct:    1 CFADVNECLSNPCQNNGICLNRNNKFLCQCSSGFTGTFCEIDVDECNS-RPCRQWGTC-VNTDGSFYCSCVLGFNGPLCTNNVNDCAPNPCIH-GRCVDQVNAFFCSCEEGWSGTTCDT-------------------------NIDD-------CVGNLCRNGATCVDLVKAFN----CSCAAGFTGQYCQ 153          
BLAST of slit1 vs. Ensembl Sea Lamprey
Match: JAG1 (jagged canonical Notch ligand 1 [Source:HGNC Symbol;Acc:HGNC:6188])

HSP 1 Score: 123.25 bits (308), Expect = 2.270e-28
Identity = 73/221 (33.03%), Postives = 107/221 (48.42%), Query Frame = 1
Query: 2788 CKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVG-NLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDI--------------------------------------NPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGK 3333
            C  NG+C      N+ C C   F G  C + +N C    C NGG C  +D   +++C+CP G+ G  C  +VDDC   N C+NG TC+DL N +SC+C++G++GK C  K  +C++                                       NPCQN   C ++  + YKC+C  GWEG  C ++ +DC  + C +   CVD  N Y+C C  G+ G+
Sbjct:  465 CGPNGRCLSQAGGNFTCMCNSGFRGSFCHENVNDCAEGACHNGGTC--LDGINSFQCMCPDGWDGEFCENDVDDCSNHNPCLNGGTCHDLVNDFSCECKNGWKGKTCHSKISHCDEATCNNGGECFDVGDTFRCSCPTGWEGPTCNIAQSIACMPNPCQNGATC-VISGDSYKCICQSGWEGANCTQDINDCSSHPCYNGGTCVDGTNWYRCECAAGFAGE 682          

HSP 2 Score: 121.709 bits (304), Expect = 5.793e-28
Identity = 88/298 (29.53%), Postives = 130/298 (43.62%), Query Frame = 1
Query: 2767 DSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCR--------------NGGICKNIDNY------------GNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIP---AWKP-------TVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSG 3552
            D C+  PC++  +C  + + +Y C C   F G NC    + C   PC+                G+                   GN+ C+C  GFRG  C  NV+DC    C NG TC D  NS+ C C  G+ G++C+     C++ NPC N G C  L  ND+ C C  GW+GK C      C   +C +  +C D+ + ++C C  G+ G  C I    A  P       T + +  S + I QS     N   ++   + C+ H C N G C++G         C+C  G++G
Sbjct:  392 DLCVPNPCQNGARCVNLPNGDYFCHCSENFEGTNCTLLKDHCLTSPCQVIDSCTVAVPSNTSESGVAHVTSTVCGPNGRCLSQAGGNFTCMCNSGFRGSFCHENVNDCAEGACHNGGTCLDGINSFQCMCPDGWDGEFCENDVDDCSNHNPCLNGGTCHDLV-NDFSCECKNGWKGKTCHSKISHCDEATCNNGGECFDVGDTFRCSCPTGWEGPTCNIAQSIACMPNPCQNGATCVISGDSYKCICQSGWEGANCTQDI---NDCSSHPCYNGGTCVDG----TNWYRCECAAGFAG 681          

HSP 3 Score: 117.857 bits (294), Expect = 9.454e-27
Identity = 77/209 (36.84%), Postives = 111/209 (53.11%), Query Frame = 1
Query: 2722 ICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHN-DYKCMCRPGYIGKFCEI 3345
            +C+     A  T   D C   PC + G C L   + ++C CP  + G  C    + C   PC +   CKN+  +G Y C C  G+ G  C I+V+DC G  C+NG TC DL N YSC+C+ G+ G+ C+ +   C   +PC N GQC+   ++ Y CVC  G+ G  CE + D C  N C++ ++CV+L N DY C C   + G  C +
Sbjct:  226 LCAPGWTGANCTEDIDECASSPCGNGGTC-LNLVNGFRCTCPPQWTGHTCLVDTDECDDNPCVHARSCKNL--FGGYYCGCLPGWTGQNCDIDVNDCEGK-CLNGGTCKDLVNDYSCECKPGYTGRRCETEVNECAS-HPCLNGGQCRDAIDS-YYCVCMDGFAGDSCELDVDLCVPNPCQNGARCVNLPNGDYFCHCSENFEGTNCTL 428          

HSP 4 Score: 110.923 bits (276), Expect = 1.269e-24
Identity = 63/172 (36.63%), Postives = 90/172 (52.33%), Query Frame = 1
Query: 2830 YQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEI 3345
            Y+C C   + G NC + I+ C   PC NGG C N+ N   + C CP  + G  C ++ D+C  N CV+  +C +L   Y C C  G+ G+ CDI    C     C N G C+ L N DY C C  G+ G++CE   ++C  + C +  QC D  + Y C+C  G+ G  CE+
Sbjct:  223 YKCLCAPGWTGANCTEDIDECASSPCGNGGTCLNLVN--GFRCTCPPQWTGHTCLVDTDECDDNPCVHARSCKNLFGGYYCGCLPGWTGQNCDIDVNDCE--GKCLNGGTCKDLVN-DYSCECKPGYTGRRCETEVNECASHPCLNGGQCRDAIDSYYCVCMDGFAGDSCEL 389          

HSP 5 Score: 108.612 bits (270), Expect = 7.009e-24
Identity = 89/294 (30.27%), Postives = 121/294 (41.16%), Query Frame = 1
Query: 2767 DSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCV----------------------------DLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQ 3564
            D C   PC H   C  +    Y C C   + G+NCD  +N C G+ C NGG CK++ N  +Y C C  G+ G +C   V++C  + C+NG  C D  +SY C C  GF G  C++    C   NPCQN  +C  LPN DY C CS+ +EG  C    D C  + C+    C                                 ++ CMC  G+ G FC                         +EN+ +       CA   C N G C++G  N  Q   C C  G+ G  C+
Sbjct:  279 DECDDNPCVHARSCKNL-FGGYYCGCLPGWTGQNCDIDVNDCEGK-CLNGGTCKDLVN--DYSCECKPGYTGRRCETEVNECASHPCLNGGQCRDAIDSYYCVCMDGFAGDSCELDVDLCVP-NPCQNGARCVNLPNGDYFCHCSENFEGTNCTLLKDHCLTSPCQVIDSCTVAVPSNTSESGVAHVTSTVCGPNGRCLSQAGGNFTCMCNSGFRGSFC-------------------------HENVND-------CAEGACHNGGTCLDG-INSFQ---CMCPDGWDGEFCE 531          

HSP 6 Score: 101.293 bits (251), Expect = 1.228e-21
Identity = 86/270 (31.85%), Postives = 116/270 (42.96%), Query Frame = 1
Query: 2764 CDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFG-QPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDD--CVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQ 3564
            CD C+  P   +G CS      ++C C   + G  CDK +N C   QPCRNGG C N     +Y+C C  G+ G  C I+      + N C NG +     + Y C C  G+ G  C      C   +PC N G C  L N  ++C C   W G  C  +TD+C  N C H   C +L   Y C C PG+ G+ C+I               D+N  E                   +CLN G C    ++      C+CKPGY+G  C+
Sbjct:  125 CDQCIPYPGCVHGGCS----KPWECRCDTNWGGLLCDKDLNYCGNHQPCRNGGSCSNA-QPDSYKCDCQKGYHGVNCEIDYVQHITLNNTCANGGSSVGSPSGYKCLCAPGWTGANCTEDIDECAS-SPCGNGGTCLNLVNG-FRCTCPPQWTGHTCLVDTDECDDNPCVHARSCKNLFGGYYCGCLPGWTGQNCDI---------------DVNDCEG------------------KCLNGGTC----KDLVNDYSCECKPGYTGRRCE 350          
BLAST of slit1 vs. Ensembl Nematostella
Match: EDO48851 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RHH9])

HSP 1 Score: 170.244 bits (430), Expect = 2.149e-42
Identity = 108/310 (34.84%), Postives = 157/310 (50.65%), Query Frame = 1
Query: 2767 DSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDIN---------------QSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNSIYFQKNSYIEISPPSRGFLMPRGNI 3651
            + C K PCK+ G C    H  Y C C   F GKNC++ +N C   PC+NGG+CKN   +G Y C C  GF G  C  +V++C  N C NG  C + +  YSC C++GF GK C+     C+  NPC+N G C+   +  Y C C  G+ GK CE++ ++C  N CK+   C + H  Y C C+ G+ GK CE        LKT+I  + I                ++  T +N E +V   + C+ + C N G+C    +N++    C CK G++G  C+   S   +  SY ++    R    PRGN+
Sbjct:  370 NECSKNPCKNGGVCKN-EHGGYSCACKAGFTGKNCEQDVNECSKNPCKNGGVCKN--EHGGYSCACKAGFTGKNCEQDVNECSKNPCQNGGVCKNEHGGYSCACKAGFMGKNCEQDVNECSK-NPCKNGGVCKN-EHGGYSCACKAGFTGKNCEQDVNECSKNPCKNGGVCKNEHGGYSCACKAGFTGKNCEQDG-----LKTIIHLQSIKCGVCKNEHGGYSCACKAGFTGKNCEQDV---NECSVNPCKNGGVC----KNEHGGYSCACKAGFTGKNCEQAPSKPRECRSYKKLDTADRAAGRPRGNV 662          

HSP 2 Score: 156.762 bits (395), Expect = 2.892e-38
Identity = 97/275 (35.27%), Postives = 143/275 (52.00%), Query Frame = 1
Query: 2767 DSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEI--------PAWKPTVLKTLISRRDIN-QSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQ 3564
            ++C  +PCK+ G C  +    Y C C   F GKNC++ +N C   PC+NGG+CKN   +G Y C C  GF G  C  +V++C  N C NG  C + +  YSC C++GF GK C+     C+  NPCQN G C+   +  Y C C  G+ GK CE++ ++C  N CK+   C + H  Y C C+ G+ GK CE         P     V K          ++  T +N E +V   + C+ + C N G+C    +N++    C CK G++G +C+
Sbjct:  990 ETCSSKPCKNGGTCREVNGA-YSCTCKSGFTGKNCEQDVNECSKNPCKNGGVCKN--EHGGYSCACKAGFTGKNCEQDVNECNKNPCQNGGVCKNEHGGYSCACKAGFTGKNCEQDMNECSK-NPCQNGGVCK-NEHGGYSCACKAGFTGKNCEQDVNECSKNPCKNGGVCKNEHGGYSCACKAGFTGKNCEQDMNECSKNPCQNGGVCKNKHGGYSCACKAGFTGKNCEQDV---NECSKNPCKNGGVC----KNEHGGYSCTCKAGFTGKICE 1252          

HSP 3 Score: 154.066 bits (388), Expect = 1.997e-37
Identity = 98/275 (35.64%), Postives = 141/275 (51.27%), Query Frame = 1
Query: 2767 DSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEI--------PAWKPTVLKTLISRRDIN-QSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQ 3564
            + C K PC++ G C    H  Y C C   F GKNC++ +N C   PC+NGG+CKN   +G Y C C  GF G  C  +V++C  N C NG  C + +  YSC C++GF GK C+     C+  NPCQN G C+   +  Y C C  G+ GK CE++ ++C  N CK+   C + H  Y C C+ G+ GK CE         P     V K          ++  T +N E +V   + C+ + C N G+C    +N++    C CK G++G  C+
Sbjct: 1066 NECNKNPCQNGGVCKN-EHGGYSCACKAGFTGKNCEQDMNECSKNPCQNGGVCKN--EHGGYSCACKAGFTGKNCEQDVNECSKNPCKNGGVCKNEHGGYSCACKAGFTGKNCEQDMNECSK-NPCQNGGVCKNK-HGGYSCACKAGFTGKNCEQDVNECSKNPCKNGGVCKNEHGGYSCTCKAGFTGKICEQDVNECSKNPCKNGGVCKNEHGGYSCTCKAGFTGKNCEQDV---NECSKNPCKNGGVC----KNEHGGYSCTCKAGFTGKNCE 1328          

HSP 4 Score: 151.369 bits (381), Expect = 1.288e-36
Identity = 98/275 (35.64%), Postives = 139/275 (50.55%), Query Frame = 1
Query: 2767 DSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEI--------PAWKPTVLKTLISRRDIN-QSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQ 3564
            + C K PCK+ G C    H  Y C C   F GKNC++ +N C   PC+NGG+CKN   +G Y C C  GF G  C  +V++C  N C NG  C + +  YSC C+ GF GK C+     C  +NPC+N G C+   +  Y C C  G+ GK CE++ ++C  N CK+   C + H  Y C C+ G+ GK CE         P     V K          ++  T +N E +V   + C+ + C N G+C    +N++    C CK G+ G  C+
Sbjct:  218 NECSKNPCKNGGVCKN-EHGGYSCACKVGFTGKNCEQDVNECSVNPCKNGGVCKN--EHGGYSCACKAGFTGKNCEQDVNECSVNPCKNGGVCKNEHGGYSCACKVGFTGKNCEQDVNEC-SVNPCKNGGVCK-NEHGGYSCACKAGFTGKNCEQDVNECSKNPCKNGGVCKNEHGGYSCACKAGFTGKNCEQDVNECSKNPCKNGGVCKNEHGGYSCACKAGFTGKNCEQDV---NECSKNPCQNGGVC----KNEHGGYSCACKAGFMGKNCE 480          

HSP 5 Score: 149.828 bits (377), Expect = 3.622e-36
Identity = 85/222 (38.29%), Postives = 118/222 (53.15%), Query Frame = 1
Query: 2767 DSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCE----------IPAW----KPTVLKTLISRR 3390
            + C K PC++ G C    H  Y C C   F GK C++ +N C   PC+NGG+CKN   +G Y C C  GF G  C  +V++C  N C NG  C + +  YSC C++GF GK C+     CN  NPCQN G C+   +  Y C C  G+ GK CE++ ++C  N CK+   C + H  Y C C+ G+ GK CE           P W     PTV + +++R+
Sbjct:  728 NECSKNPCQNGGVCKN-EHGGYSCACKAGFTGKICEQDVNECNKNPCQNGGVCKN--EHGGYSCTCKAGFTGKNCEQDVNECNKNPCQNGGVCKNEHGGYSCTCKAGFTGKNCEQDVNECNK-NPCQNGGVCK-NEHGGYSCTCKAGFTGKNCEQDVNECSKNPCKNGGVCKNEHGGYSCACKAGFTGKICEQEICEICGTHAPGWMEGSHPTVAEGIVTRK 944          

HSP 6 Score: 147.902 bits (372), Expect = 1.358e-35
Identity = 96/271 (35.42%), Postives = 139/271 (51.29%), Query Frame = 1
Query: 2767 DSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVIS----ESVCAYHECLNNGICINGDQNKNQKSY-CKCKPGYSGHLCQ 3564
            + C K PCK+ G C    H  Y C C   F GK C++ +N C   PC+NGG+CKN   +G Y C C  GF G  C  +V++C  N C NG  C + +  YSC C++GF GK C+     C+  NPC+N G C+   +  Y C C  G+ GK CE++ ++C  N CK+   C + H  Y C C+ G+ GK          +L  + +   +  +E     +E+ V++    ++ C  + CLNNG CIN        SY C C   Y+G  C+
Sbjct: 1218 NECSKNPCKNGGVCKN-EHGGYSCTCKAGFTGKICEQDVNECSKNPCKNGGVCKN--EHGGYSCTCKAGFTGKNCEQDVNECSKNPCKNGGVCKNEHGGYSCTCKAGFTGKNCEQDVNECSK-NPCKNGGVCKN-EHGGYSCTCKAGFTGKNCEQDVNECSKNPCKNGGVCKNEHGGYSCTCKAGFTGKT------ANKMLTNVPASTRVIMAESALTAMEDTVVNARPDKNECKVNPCLNNGKCIN-----TPGSYKCNCIDEYTGKHCE 1472          

HSP 7 Score: 134.806 bits (338), Expect = 1.638e-31
Identity = 112/412 (27.18%), Postives = 165/412 (40.05%), Query Frame = 1
Query: 2281 YLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVLSFNKLQCIHQDAFKNLHN-----LKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKCD----SCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSG------------------------------------QCQLLPN--NDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKT 3375
            +  +N  Q +NE S     N       + G +   K   T  K+ E  V   +   C +    KN H       KV     N    +   + N  KN          + C C        F  +  +  ++ C S    K   +  ++   + C+   +  +    C+     C K PCK+ G C    H  Y C C   F GKNC++ +N C   PC+NGG+CKN   +G Y C C  GF G  C  +V++C  N C NG  C + +  YSC C++GF GK C+     C+  NPC+N G                                    +C +  N    Y C C  G+ GK CE++ ++C  N CK+   C + H  Y C C+ G+ GK CE    KP   ++
Sbjct:  246 FTGKNCEQDVNECSVNPCKNGGVCKNEHGGYSCACKAGFT-GKNCEQDVNECSVNPCKNGGVCKNEHGGYSCACKVGFTGKNCEQDVNECSVNPCKNGGVCKNEHGGYSCACKA-----GFTGKNCEQDVNEC-SKNPCKNGGVCKNEHGGYSCA--CKAGFTGKNCEQDVNECSKNPCKNGGVCKN-EHGGYSCACKAGFTGKNCEQDVNECSKNPCQNGGVCKN--EHGGYSCACKAGFMGKNCEQDVNECSKNPCKNGGVCKNEHGGYSCACKAGFTGKNCEQDVNECSK-NPCKNGGVCKNEHGGYSCACKAGFTGKNCEQDGLKTIIHLQSIKCGVCKNEHGGYSCACKAGFTGKNCEQDVNECSVNPCKNGGVCKNEHGGYSCACKAGFTGKNCEQAPSKPRECRS 644          

HSP 8 Score: 129.413 bits (324), Expect = 5.770e-30
Identity = 83/237 (35.02%), Postives = 122/237 (51.48%), Query Frame = 1
Query: 2881 INACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEI--------PAWKPTVLKTLISRRDIN-QSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQ 3564
            +N C   PC+NGG+CKN   +G Y C C VGF G  C  +V++C  N C NG  C + +  YSC C++GF GK C+     C  +NPC+N G C+   +  Y C C  G+ GK CE++ ++C  N CK+   C + H  Y C C+ G+ GK CE         P     V K          ++  T +N E +V   + C+ + C N G+C    +N++    C CK G++G  C+
Sbjct:  217 VNECSKNPCKNGGVCKN--EHGGYSCACKVGFTGKNCEQDVNECSVNPCKNGGVCKNEHGGYSCACKAGFTGKNCEQDVNEC-SVNPCKNGGVCK-NEHGGYSCACKVGFTGKNCEQDVNECSVNPCKNGGVCKNEHGGYSCACKAGFTGKNCEQDVNECSKNPCKNGGVCKNEHGGYSCACKAGFTGKNCEQDV---NECSKNPCKNGGVC----KNEHGGYSCACKAGFTGKNCE 442          

HSP 9 Score: 122.479 bits (306), Expect = 8.407e-28
Identity = 78/227 (34.36%), Postives = 108/227 (47.58%), Query Frame = 1
Query: 2767 DSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCER------------------------------NTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWK 3357
            + C K PCK+ G C    H  Y C C   F GKNC++ +N C   PC+NGG+CKN   +G Y C C  GF G  C  +V++C  N C NG  C + +  YSC C++GF GK C+     C+  NPC+N G C+   +  Y C C  G+ GK   +                              + ++CK N C +  +C++    YKC C   Y GK CE   +K
Sbjct: 1256 NECSKNPCKNGGVCKN-EHGGYSCTCKAGFTGKNCEQDVNECSKNPCKNGGVCKN--EHGGYSCTCKAGFTGKNCEQDVNECSKNPCKNGGVCKNEHGGYSCTCKAGFTGKNCEQDVNECSK-NPCKNGGVCK-NEHGGYSCTCKAGFTGKTANKMLTNVPASTRVIMAESALTAMEDTVVNARPDKNECKVNPCLNNGKCINTPGSYKCNCIDEYTGKHCETAKYK 1477          
BLAST of slit1 vs. Ensembl Nematostella
Match: EDO46513 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RPI0])

HSP 1 Score: 148.288 bits (373), Expect = 1.000e-39
Identity = 91/252 (36.11%), Postives = 132/252 (52.38%), Query Frame = 1
Query: 2767 DSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEI--------PAWKPTVLKTLISRRDIN-QSEMTYENLENEVISESVCAYHECLNNGICIN 3495
            ++C  +PCK+ G C  +    Y C C   F GKNC++ +N C   PC+NGG+CKN   +G Y C C  GF G  C  +V++C  N C NG  C + +  YSC C++GF GK C+     C++ NPC+N G C+   +  Y C C  G+ GK CE++ ++C  N C++   C + H  Y C C+ G+ GK CE         P     V K          ++  T +N E +   ++ C  + CLNNG CIN
Sbjct:    2 ETCSSKPCKNGGTCREVNGA-YSCTCKSGFTGKNCEQDVNECSKNPCKNGGVCKN--EHGGYSCACKAGFTGKNCEQDVNECSENPCKNGGVCKNEHGGYSCACKAGFTGKNCEQDVNECSE-NPCKNGGVCK-NEHGGYSCACKAGFTGKNCEQDVNECSKNPCQNGGVCKNEHGGYSCACKAGFTGKICEQDVNECNKNPCQNGGVCKNEHGGYSCTCKAGFTGKNCEQD---KNECKVNPCLNNGKCIN 245          

HSP 2 Score: 132.494 bits (332), Expect = 2.925e-34
Identity = 75/182 (41.21%), Postives = 103/182 (56.59%), Query Frame = 1
Query: 2767 DSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMC 3312
            + C + PCK+ G C    H  Y C C   F GKNC++ +N C   PC+NGG+CKN   +G Y C C  GF G  C  +V++C  N C NG  C + +  YSC C++GF GK C+     CN  NPCQN G C+   +  Y C C  G+ GK CE++ ++CK N C +  +C++    YKC C
Sbjct:   78 NECSENPCKNGGVCKN-EHGGYSCACKAGFTGKNCEQDVNECSENPCKNGGVCKN--EHGGYSCACKAGFTGKNCEQDVNECSKNPCQNGGVCKNEHGGYSCACKAGFTGKICEQDVNECNK-NPCQNGGVCK-NEHGGYSCTCKAGFTGKNCEQDKNECKVNPCLNNGKCINTPGSYKCNC 254          

HSP 3 Score: 117.087 bits (292), Expect = 5.336e-29
Identity = 74/228 (32.46%), Postives = 115/228 (50.44%), Query Frame = 1
Query: 2881 INACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQ 3564
            +  C  +PC+NGG C+ ++  G Y C C  GF G  C  +V++C  N C NG  C + +  YSC C++GF GK C+     C++ NPC+N G C+   +  Y C C  G+ GK CE++ ++C  N CK+   C + H  Y C C+ G+ GK CE               +D+N+                 C+ + C N G+C    +N++    C CK G++G +C+
Sbjct:    1 LETCSSKPCKNGGTCREVN--GAYSCTCKSGFTGKNCEQDVNECSKNPCKNGGVCKNEHGGYSCACKAGFTGKNCEQDVNECSE-NPCKNGGVCK-NEHGGYSCACKAGFTGKNCEQDVNECSENPCKNGGVCKNEHGGYSCACKAGFTGKNCE---------------QDVNE-----------------CSKNPCQNGGVC----KNEHGGYSCACKAGFTGKICE 188          
BLAST of slit1 vs. Ensembl Nematostella
Match: EDO39165 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SB01])

HSP 1 Score: 152.91 bits (385), Expect = 3.521e-37
Identity = 92/266 (34.59%), Postives = 132/266 (49.62%), Query Frame = 1
Query: 2767 DSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQ 3564
            D C   PCKH G C       + C C + + G  C+  +N C   PC NGG+C   D  G Y+C+CP GF G  C +N+D+C  + C NGATC DL + + C+CQ GF+G+ C+     C     C N   C  L N  Y+C C  G+ G+ C+ + D+C+ N C+H S C+D  + Y+C+C+PGY G++C I               DI++                 C    CLNNG+C+    +     +C C  G+SG  C 
Sbjct:  130 DECQSSPCKHQGTCQNYLG-GFNCLCRHGYSGITCEVDVNECSSSPCVNGGVCA--DGLGEYKCICPSGFSGENCQVNIDECASSPCKNGATCEDLVDEFRCQCQPGFKGQNCETNINECIGA-ACANGSTCIDLVNA-YQCTCLPGFTGRHCDVDIDECQSNPCQHGSACMDGVSSYQCICQPGYTGQYCHI---------------DIDE-----------------CLSRPCLNNGMCL----DLVSDFHCTCPTGFSGKDCS 354          

HSP 2 Score: 144.821 bits (364), Expect = 1.125e-34
Identity = 116/358 (32.40%), Postives = 157/358 (43.85%), Query Frame = 1
Query: 2767 DSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIP---------AWKPTVLKTLIS--------------RRDINQSEM------TYENLENE--------------VISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQ-----------YLNSIYFQ-KNSYIEISPPSRGFLMPRGNISLNFMTNS 3675
            D C   PCK+   C  +    ++C C   F G+NC+  IN C G  C NG  C  ID    Y+C C  GF G  C +++D+C  N C +G+ C D  +SY C CQ G+ G+YC I    C    PC N+G C  L  +D+ C C  G+ GK C  N DDC    C+H S C+DL NDYKC C  GY GK C I           +  T L  L                 +DI+          T E+L N                 + SVC  + C N G C +   N +    C+C  GY+GH C+           Y N+      N+Y  I P  RGF     +++++   +S
Sbjct:  206 DECASSPCKNGATCEDLVDE-FRCQCQPGFKGQNCETNINECIGAACANGSTC--IDLVNAYQCTCLPGFTGRHCDVDIDECQSNPCQHGSACMDGVSSYQCICQPGYTGQYCHIDIDECLS-RPCLNNGMCLDLV-SDFHCTCPTGFSGKDCSVNIDDCADAPCQHGSTCIDLVNDYKCSCLQGYTGKDCHIDINECSSNPCQYGGTCLNLLNGYACLCIDGYTGLNCEKDIDDCLFNPCHNGTCEDLVNNYKCVCPDNRQERTCAGNSSVCDTYLCRNGGSCFS---NNSTYYTCECPKGYTGHDCESKINYCKSSSCYGNATCLDGANNYTCICP--RGFTGTLCDVNVDECEDS 553          

HSP 3 Score: 139.428 bits (350), Expect = 5.722e-33
Identity = 93/272 (34.19%), Postives = 131/272 (48.16%), Query Frame = 1
Query: 2767 DSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPA--WKPTVLKTLISRRDINQSE----MTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQ 3564
            D C   PC + G C   T++ ++C C   + G  C+  I+ C  +PC + G C +  NY  + C C VG+ G  C +N+DDC  + C +G +C DL N Y C C +GF+G  C+     C   NPC N+G C +     Y CVC  G+ G +CE N DDC  + C + + C DL ND+ C C  GY G+ C I      P   K   +  D         M      N  I    C  + CLNNG C++G      +  C C  G++G  C+
Sbjct:  624 DECSSSPCVNGGLCVDYTNY-FECLCHPGYGGDRCEINIDDCANKPCLHNGNCTDYVNY--FTCACAVGYTGRTCEVNIDDCSPSPCRHGGSCQDLVNGYLCHCPAGFKGSKCETDIDECA-TNPCDNNGTC-VDRVASYDCVCKPGFTGTRCEANIDDCATSPCLNNAICTDLINDFHCACPAGYQGQTCAINVDDCTPQPCKQGGTCVDAVSGYTCLCMPGFTGINCSIEMDECGSYPCLNNGTCVDG----RNRVTCTCAVGFTGQFCE 886          

HSP 4 Score: 137.117 bits (344), Expect = 2.942e-32
Identity = 98/305 (32.13%), Postives = 147/305 (48.20%), Query Frame = 1
Query: 2692 SIYHSKPTDFICSEETEDAYITAKCDS----CMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCE--IPAWKPTVLKTLISRRDINQSEMTY-------ENLENEVISESVCAYHECLNNGICINGDQNKNQKSY-CKCKPGYSGHLCQ 3564
            S + +  T + C  E    Y    C+S    C    C  N  C L   +NY C CP  F G  CD  ++ C   PC NG  C+++ N  ++ C CP GF+G  C +++++C  + C+N A C D+ + Y C C+SG+ G  C+     C+  +PC N G C +   N ++C+C  G+ G +CE N DDC    C H   C D  N + C C  GY G+ CE  I    P+  +   S +D+    + +          E ++     CA + C NNG C++        SY C CKPG++G  C+
Sbjct:  483 SCFSNNSTYYTC--ECPKGYTGHDCESKINYCKSSSCYGNATC-LDGANNYTCICPRGFTGTLCDVNVDECEDSPCANGASCEDLIN--DFRCHCPAGFQGRTCAVDINECATSPCLNEAKCTDVVSGYRCTCRSGYTGTRCERDIDECSS-SPCVNGGLC-VDYTNYFECLCHPGYGGDRCEINIDDCANKPCLHNGNCTDYVNYFTCACAVGYTGRTCEVNIDDCSPSPCRHGGSCQDLVNGYLCHCPAGFKGSKCETDI---DECATNPCDNNGTCVD-----RVASYDCVCKPGFTGTRCE 772          

HSP 5 Score: 136.732 bits (343), Expect = 3.859e-32
Identity = 90/272 (33.09%), Postives = 126/272 (46.32%), Query Frame = 1
Query: 2767 DSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCE--IPAWKPTVLKTLISRRDI--NQSEMTYENLENEVISESV--CAYHECLNNGICINGDQNKNQKSY-CKCKPGYSGHLC 3561
            D C   PC+H G C  + +  Y C CP  F G  C+  I+ C   PC N G C  +D   +Y+CVC  GF G +C  N+DDC  + C+N A C DL N + C C +G++G+ C I    C    PC+  G C +   + Y C+C  G+ G  C    D+C    C +   CVD  N   C C  G+ G+FCE  I    P   +   + +D+  + +   Y     E  +  +  C  H C NN  C    +    K Y C C  G +G  C
Sbjct:  700 DDCSPSPCRHGGSCQDLVN-GYLCHCPAGFKGSKCETDIDECATNPCDNNGTC--VDRVASYDCVCKPGFTGTRCEANIDDCATSPCLNNAICTDLINDFHCACPAGYQGQTCAINVDDCTP-QPCKQGGTC-VDAVSGYTCLCMPGFTGINCSIEMDECGSYPCLNNGTCVDGRNRVTCTCAVGFTGQFCEVNIDDCDPAPCQNYGTCQDLVADFTCHCYPGFTGETCATPIDLCEAHPCQNNATC----RMDPLKGYSCSCPTGVTGRHC 962          

HSP 6 Score: 135.961 bits (341), Expect = 6.474e-32
Identity = 73/208 (35.10%), Postives = 109/208 (52.40%), Query Frame = 1
Query: 2722 ICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEI 3345
            +C +  ++         C    C++ G C       Y C+CP  + G +C+ +IN C    C     C  +D   NY C+CP GF G  C +NVD+C  + C NGA+C DL N + C C +GF+G+ C +    C   +PC N  +C  + +  Y+C C  G+ G +CER+ D+C  + C +   CVD  N ++C+C PGY G  CEI
Sbjct:  456 VCPDNRQERTCAGNSSVCDTYLCRNGGSCFSNNSTYYTCECPKGYTGHDCESKINYCKSSSCYGNATC--LDGANNYTCICPRGFTGTLCDVNVDECEDSPCANGASCEDLINDFRCHCPAGFQGRTCAVDINECA-TSPCLNEAKCTDVVSG-YRCTCRSGYTGTRCERDIDECSSSPCVNGGLCVDYTNYFECLCHPGYGGDRCEI 659          

HSP 7 Score: 128.642 bits (322), Expect = 9.635e-30
Identity = 91/272 (33.46%), Postives = 120/272 (44.12%), Query Frame = 1
Query: 2767 DSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIP---AWKPTVLKTLISRRDI--NQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSY-CKCKPGYSGHLCQ 3564
            D C   PC +NG C +    +Y C C   F G  C+  I+ C   PC N  IC ++ N  ++ C CP G++G  C INVDDC    C  G TC D  + Y+C C  GF G  C I+   C    PC N+G C +   N   C C+ G+ G+ CE N DDC    C++   C DL  D+ C C PG+ G+ C  P               R D     S      +       S+C    C NNG C+       Q  Y C C   ++   CQ
Sbjct:  738 DECATNPCDNNGTC-VDRVASYDCVCKPGFTGTRCEANIDDCATSPCLNNAICTDLIN--DFHCACPAGYQGQTCAINVDDCTPQPCKQGGTCVDAVSGYTCLCMPGFTGINCSIEMDECGSY-PCLNNGTC-VDGRNRVTCTCAVGFTGQFCEVNIDDCDPAPCQNYGTCQDLVADFTCHCYPGFTGETCATPIDLCEAHPCQNNATCRMDPLKGYSCSCPTGVTGRHCDVSICDSKPCRNNGSCV-----LTQSGYQCLCASSFTSPQCQ 999          

HSP 8 Score: 127.487 bits (319), Expect = 2.346e-29
Identity = 89/299 (29.77%), Postives = 137/299 (45.82%), Query Frame = 1
Query: 2716 DFICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQC-VDLHNDYKCMCRPGYIGKFCEIPAW--KP-----TVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNSIYFQ 3588
            D +C          A  D C   PC +N  C+ + + ++ C CP  + G+ C   ++ C  QPC+ GG C  +D    Y C+C  GF G  C+I +D+C    C+N  TC D  N  +C C  GF G++C++    C D  PCQN G CQ L   D+ C C  G+ G+ C    D C+ + C++ + C +D    Y C C  G  G+ C++     KP     + + T    + +  S  T    +  +     C +  CLN   C++ D        C C  GY G  C+ ++  + Q
Sbjct:  759 DCVCKPGFTGTRCEANIDDCATSPCLNNAICTDLIN-DFHCACPAGYQGQTCAINVDDCTPQPCKQGGTC--VDAVSGYTCLCMPGFTGINCSIEMDECGSYPCLNNGTCVDGRNRVTCTCAVGFTGQFCEVNIDDC-DPAPCQNYGTCQDLVA-DFTCHCYPGFTGETCATPIDLCEAHPCQNNATCRMDPLKGYSCSCPTGVTGRHCDVSICDSKPCRNNGSCVLTQSGYQCLCASSFTSPQCQRSIDD---CEHAHCLNGATCVDSD----NAYKCSCLEGYYGKHCELIDRCHSQ 1045          

HSP 9 Score: 122.865 bits (307), Expect = 5.923e-28
Identity = 89/287 (31.01%), Postives = 132/287 (45.99%), Query Frame = 1
Query: 2767 DSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCN-DLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNSIYFQKNSYIEISPPSR 3624
            D C   PC +NG C +   +   C C   F G+ C+  I+ C   PC+N G C+++    ++ C C  GF G  C   +D C  + C N ATC  D    YSC C +G  G++CD+      D  PC+N+G C +L  + Y+C+C+  +   QC+R+ DDC++  C + + CVD  N YKC C  GY GK CE           LI R                      C    C N   C++ D       +CKC  G++G  C+   +    + +   ++ PSR
Sbjct:  852 DECGSYPCLNNGTC-VDGRNRVTCTCAVGFTGQFCEVNIDDCDPAPCQNYGTCQDL--VADFTCHCYPGFTGETCATPIDLCEAHPCQNNATCRMDPLKGYSCSCPTGVTGRHCDVSIC---DSKPCRNNGSC-VLTQSGYQCLCASSFTSPQCQRSIDDCEHAHCLNGATCVDSDNAYKCSCLEGYYGKHCE-----------LIDR----------------------CHSQPCQNGATCVDDD----GVVFCKCAMGFTGTHCEKEFNHCIVRVADTNLTFPSR 1094          

HSP 10 Score: 114.005 bits (284), Expect = 2.726e-25
Identity = 90/323 (27.86%), Postives = 138/323 (42.72%), Query Frame = 1
Query: 2773 CMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPN-------------------------------------NDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCE--------------------IPAWKPTVLKTLISRR-DINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSY-CKCKPGYSGHLCQ 3564
            C    C + G CS  ++ +  C C   F G++C+  ++ C    C NG  C  ID   +++C+CPVGF G  C  N +DC  N C N ATC DL N +SC+C  G  G+ C+     C   +PC++ G CQ                                          +YKC+C  G+ G+ C+ N D+C  + CK+ + C DL ++++C C+PG+ G+ CE                    + A++ T L     R  D++  E               C  + C +   C++G       SY C C+PGY+G  C 
Sbjct:   18 CQHDSCLNGGTCSEGSN-SLLCSCLPGFTGQSCETDVDECKLVQCHNGATC--IDQVNSFKCICPVGFHGILCETNYNDCHSNPCQNNATCQDLINDFSCQCPQGITGQTCNTDIDECQS-SPCKHQGTCQNYLGGFNCLCRHGYSGITCEVDVNECSSSPCVNGGVCADGLGEYKCICPSGFSGENCQVNIDECASSPCKNGATCEDLVDEFRCQCQPGFKGQNCETNINECIGAACANGSTCIDLVNAYQCTCLPGFTGRHCDVDIDE---------------CQSNPCQHGSACMDG-----VSSYQCICQPGYTGQYCH 316          

HSP 11 Score: 112.464 bits (280), Expect = 9.708e-25
Identity = 104/361 (28.81%), Postives = 144/361 (39.89%), Query Frame = 1
Query: 2626 FLDRFLDNGISLCAS-PEKMKLKSIYHSKPTDFICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCV---------------------------------------CSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEI--------PAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQ 3564
            F  R  D  I  C S P +     +        IC       Y     D C+ RPC +NG C L    ++ C CP  F GK+C   I+ C   PC++G  C  ID   +Y+C C  G+ G  C I++++C  N C  G TC +L N Y+C C  G+ G  C+     C   NPC N G C+ L NN YKCV                                       C KG+ G  CE   + CK +SC   + C+D  N+Y C+C  G+ G  C++        P       + LI+  D               +  + CA   CLN   C     +      C C+ GY+G  C+
Sbjct:  272 FTGRHCDVDIDECQSNPCQHGSACMDGVSSYQCICQPGYTGQYCHIDIDECLSRPCLNNGMC-LDLVSDFHCTCPTGFSGKDCSVNIDDCADAPCQHGSTC--IDLVNDYKCSCLQGYTGKDCHIDINECSSNPCQYGGTCLNLLNGYACLCIDGYTGLNCEKDIDDCL-FNPCHN-GTCEDLVNN-YKCVCPDNRQERTCAGNSSVCDTYLCRNGGSCFSNNSTYYTCECPKGYTGHDCESKINYCKSSSCYGNATCLDGANNYTCICPRGFTGTLCDVNVDECEDSPCANGASCEDLIN--DFRCHCPAGFQGRTCAVDINECATSPCLNEAKCT----DVVSGYRCTCRSGYTGTRCE 620          

HSP 12 Score: 105.916 bits (263), Expect = 9.537e-23
Identity = 89/332 (26.81%), Postives = 135/332 (40.66%), Query Frame = 1
Query: 2401 PLKSLETLVLSFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFIC------SEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDC-----------KYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEI 3345
            P K   T V + +   C+    F  ++      ++ ++  + P        N      G+N   C C + +  QF      D        P   +          DF C      + ET    I    D C   PC++N  C +     Y C CP    G++CD  I  C  +PCRN G C  +     Y+C+C   F  P+C  ++DDC    C+NGATC D +N+Y C C  G+ GK+C++    C+   PCQN   C +  +    C C+ G+ G  CE+  + C               C     CV     + C+C+PG+ G  C+ 
Sbjct:  820 PCKQGGTCVDAVSGYTCLCMPGFTGIN----CSIEMDECGSYP------CLNNGTCVDGRNRVTCTCAVGFTGQFCEVNIDD------CDPAPCQNYGTCQDLVADFTCHCYPGFTGETCATPI----DLCEAHPCQNNATCRMDPLKGYSCSCPTGVTGRHCDVSI--CDSKPCRNNGSC--VLTQSGYQCLCASSFTSPQCQRSIDDCEHAHCLNGATCVDSDNAYKCSCLEGYYGKHCEL-IDRCHS-QPCQNGATC-VDDDGVVFCKCAMGFTGTHCEKEFNHCIVRVADTNLTFPSRICGDHGTCVSKPTGFLCVCQPGFTGLLCQT 1124          

HSP 13 Score: 87.4261 bits (215), Expect = 4.072e-17
Identity = 65/190 (34.21%), Postives = 90/190 (47.37%), Query Frame = 1
Query: 2773 CMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYC----NDIN------PCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKH-ESQCVDLHNDYKCM 3309
            C  +PC++NG C ++T   YQC C   F    C + I+ C    C NG  C + DN   Y+C C  G+ G  C + +D C    C NGATC D +    CKC  GF G +C+ +F +C     D N       C + G C   P   + CVC  G+ G  C+ + DDC  + C      CVD  + + C 
Sbjct:  967 CDSKPCRNNGSC-VLTQSGYQCLCASSFTSPQCQRSIDDCEHAHCLNGATCVDSDNA--YKCSCLEGYYGKHCEL-IDRCHSQPCQNGATCVDDDGVVFCKCAMGFTGTHCEKEFNHCIVRVADTNLTFPSRICGDHGTCVSKPTG-FLCVCQPGFTGLLCQTDIDDCSSDPCVGINVTCVDGIDTFTCQ 1151          
BLAST of slit1 vs. Ensembl Nematostella
Match: EDO42644 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S0V1])

HSP 1 Score: 139.428 bits (350), Expect = 5.396e-37
Identity = 83/193 (43.01%), Postives = 101/193 (52.33%), Query Frame = 1
Query: 2767 DSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEI 3345
            D C   PCK+ G C +   + Y C C   F GKNC+  I+ C G PC NGG CK  D    + C C VG+ G  C I++D+C    C NGATCND  N+Y+C C  GF G  C+     C    PCQN G C    NN Y C+C  G+ G  CE N D+C  N C +   C D  N + C C  GY G  CEI
Sbjct:   34 DECKSSPCKNGGTC-VDGINRYSCTCVAGFTGKNCETDIDECAGNPCANGGTCK--DGINGFTCECAVGYNGSTCEIDIDECASGPCQNGATCNDGVNNYTCSCIPGFNGTNCETNIDECAS-GPCQNGGTCNDGVNN-YTCLCIPGFNGTNCETNIDECIGNPCANGGTCKDGINGFTCECPVGYSGSTCEI 221          

HSP 2 Score: 122.094 bits (305), Expect = 4.692e-31
Identity = 82/244 (33.61%), Postives = 109/244 (44.67%), Query Frame = 1
Query: 2833 QCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQ 3564
            +C CP  F G  C+   + C   PC+NGG C  +D    Y C C  GF G  C  ++D+C GN C NG TC D  N ++C+C  G+ G  C+I    C    PCQN   C    NN Y C C  G+ G  CE N D+C    C++   C D  N+Y C+C PG+ G  CE                +I++                 C  + C N G C +G         C+C  GYSG  C+
Sbjct:   17 RCHCPAGFSGDKCENHDDECKSSPCKNGGTC--VDGINRYSCTCVAGFTGKNCETDIDECAGNPCANGGTCKDGINGFTCECAVGYNGSTCEIDIDECAS-GPCQNGATCNDGVNN-YTCSCIPGFNGTNCETNIDECASGPCQNGGTCNDGVNNYTCLCIPGFNGTNCET---------------NIDE-----------------CIGNPCANGGTCKDGIN----GFTCECPVGYSGSTCE 220          
BLAST of slit1 vs. Ensembl Nematostella
Match: EDO36195 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SJC2])

HSP 1 Score: 140.969 bits (354), Expect = 1.304e-36
Identity = 97/269 (36.06%), Postives = 124/269 (46.10%), Query Frame = 1
Query: 2773 CMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDI-----NPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQ 3564
            C   PC++ G C +     Y C C   ++G NC+  IN C   PC NGG C   D    YECVC  G+ G +C I++D+C  + CVNG TC D  N+Y+C C  GF G +     + C DI     NPC N G C  + N  Y C C  G+ G  CE + ++C  N C +   C DL N Y C C PGY G  CEI               DIN+                 CA + C+N G C +     N    C C PGY G  C+
Sbjct:   79 CTSAPCQNGGSC-VDRRDGYTCTCEAGYNGINCEIEINECDSGPCNNGGTCT--DRVNGYECVCNAGYTGTRCEIDIDECTSSPCVNGGTCTDAVNNYTCACLPGFTGYF----IVTCADIDECASNPCINGGTCTDMVNG-YNCTCPPGYNGTTCEIDINECASNPCLNGGTCNDLVNGYNCNCPPGYNGTTCEI---------------DINE-----------------CASNPCVNGGTCADLVNGFN----CTCPPGYKGTKCE 303          

HSP 2 Score: 129.028 bits (323), Expect = 1.678e-32
Identity = 81/265 (30.57%), Postives = 124/265 (46.79%), Query Frame = 1
Query: 2773 CMKRPCKHNGKCSL-ITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQ 3564
            C    C H G C    +    +C C   + G++C+  ++ C   PC N G C  +D   ++ C CP+GF G +C   + +C    C NG +C D  + Y+C C++G+ G  C+I+   C D  PC N G C    N  Y+CVC+ G+ G +CE + D+C  + C +   C D  N+Y C C PG+ G F             +++  DI++                 CA + C+N G C +     N    C C PGY+G  C+
Sbjct:    1 CASNKCVHGGTCEFDCSTLTSRCRCVAGYTGEHCEVNVDDCASSPCTNNGTC--VDGINSFACTCPLGFTGTRCETEIKECTSAPCQNGGSCVDRRDGYTCTCEAGYNGINCEIEINEC-DSGPCNNGGTCTDRVNG-YECVCNAGYTGTRCEIDIDECTSSPCVNGGTCTDAVNNYTCACLPGFTGYF-------------IVTCADIDE-----------------CASNPCINGGTCTDMVNGYN----CTCPPGYNGTTCE 227          

HSP 3 Score: 100.138 bits (248), Expect = 1.286e-22
Identity = 63/157 (40.13%), Postives = 82/157 (52.23%), Query Frame = 1
Query: 2767 DSCMKRPCKHNGKCSLITHHNYQCDCPYPFHG---KNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCE 3228
            D C   PC + G C+   + NY C C   F G     C   I+ C   PC NGG C ++ N   Y C CP G+ G  C I++++C  N C+NG TCNDL N Y+C C  G+ G  C+I    C   NPC N G C  L N  + C C  G++G +CE
Sbjct:  153 DECTSSPCVNGGTCTDAVN-NYTCACLPGFTGYFIVTC-ADIDECASNPCINGGTCTDMVN--GYNCTCPPGYNGTTCEIDINECASNPCLNGGTCNDLVNGYNCNCPPGYNGTTCEIDINECAS-NPCVNGGTCADLVNG-FNCTCPPGYKGTKCE 303          

HSP 4 Score: 92.0485 bits (227), Expect = 7.337e-20
Identity = 48/120 (40.00%), Postives = 67/120 (55.83%), Query Frame = 1
Query: 2767 DSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYC 3126
            D C   PC + G C+ + +  Y C CP  ++G  C+  IN C   PC NGG C ++ N   Y C CP G+ G  C I++++C  N CVNG TC DL N ++C C  G++G  C+I    C
Sbjct:  193 DECASNPCINGGTCTDMVN-GYNCTCPPGYNGTTCEIDINECASNPCLNGGTCNDLVN--GYNCNCPPGYNGTTCEIDINECASNPCVNGGTCADLVNGFNCTCPPGYKGTKCEIGKCVC 309          
BLAST of slit1 vs. Ensembl Medaka
Match: slit2 (slit guidance ligand 2 [Source:NCBI gene;Acc:101173607])

HSP 1 Score: 711.835 bits (1836), Expect = 0.000e+0
Identity = 474/1409 (33.64%), Postives = 714/1409 (50.67%), Query Frame = 1
Query:   43 MIFIFKDITFKFLLVLLSSLYCFSSTIWINDPSDESEICPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQLQ------------------------CINDNIFNKLGMLKYLLLEGNKIFWISEA-ISKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCASEDKANLENMCPSDKKV-CSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQIYNFQCN--KEFESKYETHDEECVPKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLPPIYSIHEAPNLMKLDLSFNQIETLEPDVFTRSSK------------------------LREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQFHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLC-DGNASVYHSVDSCHETNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVLSFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYG-NYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCN-DINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNSIYF-QKNSYIEISPPSRGFLMPRGNISLNFMTNSSSGLILYQGSEQTYLVVNLYQGHLRISYNLGDKVVGPEG-YSMGMVNDSRLHALHIEIVGENLTMYLNGRVHCTLKSKYLNSNRYMALDTGIFIGGAPHKILQSAIMKFHIET-----DSEFIGCMESLLINRKKIDFSKYL 4083
            M+   +   +   L  +S L     TI ++  +D ++ CP  C C   +V+C  +   +VP  +   T RL+L  N +  I K+DF  L +L+VLQL  N+IT+IE GAF +L  L +LRLNRN +   P+ LF   TKL +LDL +NQ+Q                        CI D  F  L  L+ L L  N I  +S A  + +  L+  R+H N L C+CH+AWL+ +L+Q+  +   G ++  C +P  L    ++ +   EF C    + +  +   S   + C + C CS++ IV C GK L+ IP +LP  + E+ L+ N I ++  G  S YK L++I L+ N + ++   AF GL  L S+ L  N +  I K  F GL SL  L L  N I CL  D  +    L+LL L +NKL ++ K TF     ++ L+L  NP  CDC + W+ DY++D    T  A C  PR L    +  ++   F+C+  +++ SK      +C   ++CP KC C    V+CSN  L  +P+ I   T  L +++N    L       + P+L K++LS N+I  +E   F  +S                         LR + L SNR+ C++N +F  L  + +L L +N ++ +   +F    +L  L L  N F+C+C L W+ +W R    V GN   P CQ+P  LK+ PI  +  + F C DGN     + +SC         CS                    T V CS+  L  +P  +PK+TTELYLD NH   +      N  +L  + LS N I+ L+ + L+ +  L TL+LS+N+L+CI   AF  L +L++L L  ND+S IP  AF DL +L+++ALG NP +CDC+++WL+ +    + + GI+ CA P  M  K +  +    F C+    D  I AKC+ C+  PCK++G C+    H Y+C CPY F G+NC+  I+AC   PC+NGG C   +  G N+ CVCP GF G  C IN+DDC  N C N +TC D  N+Y+C C   + G+ C+ K  +C  ++NPCQ+  +C L P   YKC C+ G+ G+ CE + DDCK N C++ +QC+D  N Y C+C  GY G FCE     P VL                          S C  HECLN   C+    +      C+C  GY G  C+ L S+ F  + SY+++  PS   L P+ NISL   T+  SG++LY+G +  ++ + LY+G LR+SY+ G     P   YS+  VND   HA+ +    + L++ ++G    ++ S  +N    + +D+ +++GG P +   S        +      + F GC+ +L IN K  D    L
Sbjct:    1 MVGALRPAAYSRSLATVSGLLVI--TILLSSGAD-AQPCPTQCSCTGTTVDCHGQGLRSVPRNIPRNTERLDLNANNLTKITKADFAGLRHLRVLQLMENKITTIERGAFQDLKELERLRLNRNNLAVFPELLFLGTTKLHRLDLSENQIQGVPRKAFRGAVEIKNLQLDYNHISCIEDGAFRALRDLEVLTLNNNNISRLSVASFNHMPKLRTFRLHSNNLQCDCHVAWLSEWLRQRPRL---GLYTQ-CMSPPHLRGHNVAEVQKKEFVCTDAVEGHQSSSSSSCSVLQCPESCTCSNN-IVDCRGKGLTEIPTNLPETITEIRLEQNAIKVIPAGAFSPYKRLRRIDLSNNQISELASDAFQGLRSLNSLVLYGNKITEISKGLFEGLFSLQLLLLNANKIACLRVDAFQDLHNLNLLSLYDNKLQTIAKGTFSSLRAIQTLHLAQNPFICDCHLKWLADYLQDNPIETSGARCTSPRRLANKRIGQIKSKKFRCSGTEDYRSKL---GGDCFADLACPEKCRCEGTTVDCSNQKLTKIPDHIPQYTAELRLNNNEFTVLEATGIFKKLPHLRKINLSNNRITDIEEGTFEGASGVNELILTSNKLENIHHSMLKGLGGLRTLMLRSNRISCVSNSSFVGLSSVRLLSLYDNQVTSMTPGAFDTLHSLSTLNLLANPFNCNCHLAWLGDWLRRKRIVTGN---PRCQNPYFLKEIPIQDVAVQDFACEDGN-----NENSCSPVMRCPAECSC-----------------LDTVVRCSNKGLTTLPKGLPKETTELYLDGNHFTQV-PVELSNYKHLTLIDLSNNQISTLSNHSLSNMSELLTLILSYNRLRCIPVRAFDGLKSLRLLSLHGNDISLIPEGAFKDLSSLSHLALGANPLYCDCHMQWLSDWVKSGYKEPGIARCAGPGDMTDKLLLTTPSKKFTCTGPV-DLSIQAKCEPCLSNPCKNDGTCTNDPVHYYRCTCPYGFKGQNCEDPIHACISNPCQNGGTCHLKEGDGSNFWCVCPEGFEGDSCEINIDDCEDNDCENNSTCVDGINNYTCMCSPEYTGELCEEKLDFCAPELNPCQHDSKCILTPQG-YKCECTPGFVGEHCELDYDDCKDNKCQNGAQCIDAVNGYTCVCSEGYSGLFCEFAP--PMVLP-----------------------RTSPCDNHECLNGAQCVVVGTDPR----CQCLHGYEGERCETLVSVNFVNRESYLQL--PSN-LLSPQTNISLQIATDEDSGVLLYKG-DNDHIAMELYRGRLRVSYDTGS--YPPSAIYSVETVNDGSFHAVELVAAEQTLSLSIDGGPPKSIGS--INKQSTLNIDSPLYLGGMPERAFASGGFSALQHSPGGHNGTSFHGCLRNLYINGKLQDLGAGL 1333          

HSP 2 Score: 56.9954 bits (136), Expect = 1.810e-7
Identity = 44/136 (32.35%), Postives = 66/136 (48.53%), Query Frame = 1
Query: 2899 QPCRN----GGICKNIDNYGNYECVCPVGFRGPKCTINVDD-CVGNLCVNGATCNDLNN-SYSCKCQSGFRGKYCDIKFLYCNDINPCQNS----GQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQ 3276
            QPC+      G C      G + C C  G+ G  C   V + C GN C+ G TC  +N+ SYSC+CQ GF G  CD +    + +N C  S    G+C++       C C+ G+ G+ C+R + +   N  +  +Q
Sbjct: 1359 QPCQRDTCVSGDCHPTGQRG-FTCTCHPGWTGTLCDQQVSNPCDGNKCIYG-TCVPINSYSYSCRCQPGFTGVLCDERDH--DTVNSCSLSHCKHGKCRVSGLGKAYCECNSGYTGEACDRASPELPLNPMRGATQ 1490          
BLAST of slit1 vs. Ensembl Medaka
Match: slit2 (slit guidance ligand 2 [Source:NCBI gene;Acc:101173607])

HSP 1 Score: 704.901 bits (1818), Expect = 0.000e+0
Identity = 475/1425 (33.33%), Postives = 715/1425 (50.18%), Query Frame = 1
Query:   43 MIFIFKDITFKFLLVLLSSLYCFSSTIWINDPSDESEICPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQLQ------------------------CINDNIFNKLGMLKYLLLEGNKIFWISEA-ISKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCASEDKANLENMCPSDKKV-CSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQIYNFQCN----------KEFESKYETHDEECVPKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLPPIYSIHEAPNLMKLDLSFNQIETLEPDVFTRSSK------------------------LREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQFHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLC-DGNASVYHSVDSCHETNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVLSFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYG-NYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGF--------RGKYCDIKFLYCN-DINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNSIYF-QKNSYIEISPPSRGFLMPRGNISLNFMTNSSSGLILYQGSEQTYLVVNLYQGHLRISYNLGDKVVGPEG-YSMGMVNDSRLHALHIEIVGENLTMYLNGRVHCTLKSKYLNSNRYMALDTGIFIGGAPHKILQSAIMKFHIET-----DSEFIGCMESLLINRKKIDFSKYL 4083
            M+   +   +   L  +S L     TI ++  +D ++ CP  C C   +V+C  +   +VP  +   T RL+L  N +  I K+DF  L +L+VLQL  N+IT+IE GAF +L  L +LRLNRN +   P+ LF   TKL +LDL +NQ+Q                        CI D  F  L  L+ L L  N I  +S A  + +  L+  R+H N L C+CH+AWL+ +L+Q+  +   G ++  C +P  L    ++ +   EF C    + +  +   S   + C + C CS++ IV C GK L+ IP +LP  + E+ L+ N I ++  G  S YK L++I L+ N + ++   AF GL  L S+ L  N +  I K  F GL SL  L L  N I CL  D  +    L+LL L +NKL ++ K TF     ++ L+L  NP  CDC + W+ DY++D    T  A C  PR L    +  ++   F+C+          +++ SK      +C   ++CP KC C    V+CSN  L  +P+ I   T  L +++N    L       + P+L K++LS N+I  +E   F  +S                         LR + L SNR+ C++N +F  L  + +L L +N ++ +   +F    +L  L L  N F+C+C L W+ +W R    V GN   P CQ+P  LK+ PI  +  + F C DGN     + +SC         CS                    T V CS+  L  +P  +PK+TTELYLD NH   +      N  +L  + LS N I+ L+ + L+ +  L TL+LS+N+L+CI   AF  L +L++L L  ND+S IP  AF DL +L+++ALG NP +CDC+++WL+ +    + + GI+ CA P  M  K +  +    F C+    D  I AKC+ C+  PCK++G C+    H Y+C CPY F G+NC+  I+AC   PC+NGG C   +  G N+ CVCP GF G  C IN+DDC  N C N +TC D  N+Y+C C   +        RG+ C+ K  +C  ++NPCQ+  +C L P   YKC C+ G+ G+ CE + DDCK N C++ +QC+D  N Y C+C  GY G FCE     P VL                          S C  HECLN   C+    +      C+C  GY G  C+ L S+ F  + SY+++  PS   L P+ NISL   T+  SG++LY+G +  ++ + LY+G LR+SY+ G     P   YS+  VND   HA+ +    + L++ ++G    ++ S  +N    + +D+ +++GG P +   S        +      + F GC+ +L IN K  D    L
Sbjct:    1 MVGALRPAAYSRSLATVSGLLVI--TILLSSGAD-AQPCPTQCSCTGTTVDCHGQGLRSVPRNIPRNTERLDLNANNLTKITKADFAGLRHLRVLQLMENKITTIERGAFQDLKELERLRLNRNNLAVFPELLFLGTTKLHRLDLSENQIQGVPRKAFRGAVEIKNLQLDYNHISCIEDGAFRALRDLEVLTLNNNNISRLSVASFNHMPKLRTFRLHSNNLQCDCHVAWLSEWLRQRPRL---GLYTQ-CMSPPHLRGHNVAEVQKKEFVCTDAVEGHQSSSSSSCSVLQCPESCTCSNN-IVDCRGKGLTEIPTNLPETITEIRLEQNAIKVIPAGAFSPYKRLRRIDLSNNQISELASDAFQGLRSLNSLVLYGNKITEISKGLFEGLFSLQLLLLNANKIACLRVDAFQDLHNLNLLSLYDNKLQTIAKGTFSSLRAIQTLHLAQNPFICDCHLKWLADYLQDNPIETSGARCTSPRRLANKRIGQIKSKKFRCSAREQYFIPGTEDYRSKL---GGDCFADLACPEKCRCEGTTVDCSNQKLTKIPDHIPQYTAELRLNNNEFTVLEATGIFKKLPHLRKINLSNNRITDIEEGTFEGASGVNELILTSNKLENIHHSMLKGLGGLRTLMLRSNRISCVSNSSFVGLSSVRLLSLYDNQVTSMTPGAFDTLHSLSTLNLLANPFNCNCHLAWLGDWLRRKRIVTGN---PRCQNPYFLKEIPIQDVAVQDFACEDGN-----NENSCSPVMRCPAECSC-----------------LDTVVRCSNKGLTTLPKGLPKETTELYLDGNHFTQV-PVELSNYKHLTLIDLSNNQISTLSNHSLSNMSELLTLILSYNRLRCIPVRAFDGLKSLRLLSLHGNDISLIPEGAFKDLSSLSHLALGANPLYCDCHMQWLSDWVKSGYKEPGIARCAGPGDMTDKLLLTTPSKKFTCTGPV-DLSIQAKCEPCLSNPCKNDGTCTNDPVHYYRCTCPYGFKGQNCEDPIHACISNPCQNGGTCHLKEGDGSNFWCVCPEGFEGDSCEINIDDCEDNDCENNSTCVDGINNYTCMCSPEYTAATNEVERGELCEEKLDFCAPELNPCQHDSKCILTPQG-YKCECTPGFVGEHCELDYDDCKDNKCQNGAQCIDAVNGYTCVCSEGYSGLFCEFAP--PMVLP-----------------------RTSPCDNHECLNGAQCVVVGTDPR----CQCLHGYEGERCETLVSVNFVNRESYLQL--PSN-LLSPQTNISLQIATDEDSGVLLYKG-DNDHIAMELYRGRLRVSYDTGS--YPPSAIYSVETVNDGSFHAVELVAAEQTLSLSIDGGPPKSIGS--INKQSTLNIDSPLYLGGMPERAFASGGFSALQHSPGGHNGTSFHGCLRNLYINGKLQDLGAGL 1349          

HSP 2 Score: 54.6842 bits (130), Expect = 1.078e-6
Identity = 42/121 (34.71%), Postives = 60/121 (49.59%), Query Frame = 1
Query: 2899 QPCRN----GGICKNIDNYGNYECVCPVGFRGPKCTINVDD-CVGNLCVNGATCNDLNN-SYSCKCQSGFRGKYCDIKFLYCNDINPCQNS----GQCQLLPNNDYKCVCSKGWEGKQCER 3231
            QPC+      G C      G + C C  G+ G  C   V + C GN C+ G TC  +N+ SYSC+CQ GF G  CD +    + +N C  S    G+C++       C C+ G+ G+ C+R
Sbjct: 1375 QPCQRDTCVSGDCHPTGQRG-FTCTCHPGWTGTLCDQQVSNPCDGNKCIYG-TCVPINSYSYSCRCQPGFTGVLCDERDH--DTVNSCSLSHCKHGKCRVSGLGKAYCECNSGYTGEACDR 1491          
BLAST of slit1 vs. Ensembl Medaka
Match: slit2 (slit guidance ligand 2 [Source:NCBI gene;Acc:101173607])

HSP 1 Score: 703.36 bits (1814), Expect = 0.000e+0
Identity = 474/1424 (33.29%), Postives = 713/1424 (50.07%), Query Frame = 1
Query:   43 MIFIFKDITFKFLLVLLSSLYCFSSTIWINDPSDESEICPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQLQ------------------------CINDNIFNKLGMLKYLLLEGNKIFWISEA-ISKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCASEDKANLENMCPSDKKVCSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQIYNFQCN----------KEFESKYETHDEECVPKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLPPIYSIHEAPNLMKLDLSFNQIETLEPDVFTRSSK------------------------LREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQFHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLC-DGNASVYHSVDSCHETNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVLSFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYG-NYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGF--------RGKYCDIKFLYCN-DINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNSIYF-QKNSYIEISPPSRGFLMPRGNISLNFMTNSSSGLILYQGSEQTYLVVNLYQGHLRISYNLGDKVVGPEG-YSMGMVNDSRLHALHIEIVGENLTMYLNGRVHCTLKSKYLNSNRYMALDTGIFIGGAPHKILQSAIMKFHIET-----DSEFIGCMESLLINRKKIDFSKYL 4083
            M+   +   +   L  +S L     TI ++  +D ++ CP  C C   +V+C  +   +VP  +   T RL+L  N +  I K+DF  L +L+VLQL  N+IT+IE GAF +L  L +LRLNRN +   P+ LF   TKL +LDL +NQ+Q                        CI D  F  L  L+ L L  N I  +S A  + +  L+  R+H N L C+CH+AWL+ +L+Q+  +   G ++  C +P  L    ++ +   EF C     ++  +        C + C CS++ IV C GK L+ IP +LP  + E+ L+ N I ++  G  S YK L++I L+ N + ++   AF GL  L S+ L  N +  I K  F GL SL  L L  N I CL  D  +    L+LL L +NKL ++ K TF     ++ L+L  NP  CDC + W+ DY++D    T  A C  PR L    +  ++   F+C+          +++ SK      +C   ++CP KC C    V+CSN  L  +P+ I   T  L +++N    L       + P+L K++LS N+I  +E   F  +S                         LR + L SNR+ C++N +F  L  + +L L +N ++ +   +F    +L  L L  N F+C+C L W+ +W R    V GN   P CQ+P  LK+ PI  +  + F C DGN     + +SC         CS                    T V CS+  L  +P  +PK+TTELYLD NH   +      N  +L  + LS N I+ L+ + L+ +  L TL+LS+N+L+CI   AF  L +L++L L  ND+S IP  AF DL +L+++ALG NP +CDC+++WL+ +    + + GI+ CA P  M  K +  +    F C+    D  I AKC+ C+  PCK++G C+    H Y+C CPY F G+NC+  I+AC   PC+NGG C   +  G N+ CVCP GF G  C IN+DDC  N C N +TC D  N+Y+C C   +        RG+ C+ K  +C  ++NPCQ+  +C L P   YKC C+ G+ G+ CE + DDCK N C++ +QC+D  N Y C+C  GY G FCE     P VL                          S C  HECLN   C+    +      C+C  GY G  C+ L S+ F  + SY+++  PS   L P+ NISL   T+  SG++LY+G +  ++ + LY+G LR+SY+ G     P   YS+  VND   HA+ +    + L++ ++G    ++ S  +N    + +D+ +++GG P +   S        +      + F GC+ +L IN K  D    L
Sbjct:    1 MVGALRPAAYSRSLATVSGLLVI--TILLSSGAD-AQPCPTQCSCTGTTVDCHGQGLRSVPRNIPRNTERLDLNANNLTKITKADFAGLRHLRVLQLMENKITTIERGAFQDLKELERLRLNRNNLAVFPELLFLGTTKLHRLDLSENQIQGVPRKAFRGAVEIKNLQLDYNHISCIEDGAFRALRDLEVLTLNNNNISRLSVASFNHMPKLRTFRLHSNNLQCDCHVAWLSEWLRQRPRL---GLYTQ-CMSPPHLRGHNVAEVQKKEFVCTGHQSSSSSSC---SVLQCPESCTCSNN-IVDCRGKGLTEIPTNLPETITEIRLEQNAIKVIPAGAFSPYKRLRRIDLSNNQISELASDAFQGLRSLNSLVLYGNKITEISKGLFEGLFSLQLLLLNANKIACLRVDAFQDLHNLNLLSLYDNKLQTIAKGTFSSLRAIQTLHLAQNPFICDCHLKWLADYLQDNPIETSGARCTSPRRLANKRIGQIKSKKFRCSAREQYFIPGTEDYRSKL---GGDCFADLACPEKCRCEGTTVDCSNQKLTKIPDHIPQYTAELRLNNNEFTVLEATGIFKKLPHLRKINLSNNRITDIEEGTFEGASGVNELILTSNKLENIHHSMLKGLGGLRTLMLRSNRISCVSNSSFVGLSSVRLLSLYDNQVTSMTPGAFDTLHSLSTLNLLANPFNCNCHLAWLGDWLRRKRIVTGN---PRCQNPYFLKEIPIQDVAVQDFACEDGN-----NENSCSPVMRCPAECSC-----------------LDTVVRCSNKGLTTLPKGLPKETTELYLDGNHFTQV-PVELSNYKHLTLIDLSNNQISTLSNHSLSNMSELLTLILSYNRLRCIPVRAFDGLKSLRLLSLHGNDISLIPEGAFKDLSSLSHLALGANPLYCDCHMQWLSDWVKSGYKEPGIARCAGPGDMTDKLLLTTPSKKFTCTGPV-DLSIQAKCEPCLSNPCKNDGTCTNDPVHYYRCTCPYGFKGQNCEDPIHACISNPCQNGGTCHLKEGDGSNFWCVCPEGFEGDSCEINIDDCEDNDCENNSTCVDGINNYTCMCSPEYTAATNEVERGELCEEKLDFCAPELNPCQHDSKCILTPQG-YKCECTPGFVGEHCELDYDDCKDNKCQNGAQCIDAVNGYTCVCSEGYSGLFCEFAP--PMVLP-----------------------RTSPCDNHECLNGAQCVVVGTDPR----CQCLHGYEGERCETLVSVNFVNRESYLQL--PSN-LLSPQTNISLQIATDEDSGVLLYKG-DNDHIAMELYRGRLRVSYDTGS--YPPSAIYSVETVNDGSFHAVELVAAEQTLSLSIDGGPPKSIGS--INKQSTLNIDSPLYLGGMPERAFASGGFSALQHSPGGHNGTSFHGCLRNLYINGKLQDLGAGL 1345          

HSP 2 Score: 54.6842 bits (130), Expect = 1.096e-6
Identity = 42/121 (34.71%), Postives = 60/121 (49.59%), Query Frame = 1
Query: 2899 QPCRN----GGICKNIDNYGNYECVCPVGFRGPKCTINVDD-CVGNLCVNGATCNDLNN-SYSCKCQSGFRGKYCDIKFLYCNDINPCQNS----GQCQLLPNNDYKCVCSKGWEGKQCER 3231
            QPC+      G C      G + C C  G+ G  C   V + C GN C+ G TC  +N+ SYSC+CQ GF G  CD +    + +N C  S    G+C++       C C+ G+ G+ C+R
Sbjct: 1371 QPCQRDTCVSGDCHPTGQRG-FTCTCHPGWTGTLCDQQVSNPCDGNKCIYG-TCVPINSYSYSCRCQPGFTGVLCDERDH--DTVNSCSLSHCKHGKCRVSGLGKAYCECNSGYTGEACDR 1487          
BLAST of slit1 vs. Ensembl Medaka
Match: slit2 (slit guidance ligand 2 [Source:NCBI gene;Acc:101173607])

HSP 1 Score: 682.945 bits (1761), Expect = 0.000e+0
Identity = 469/1425 (32.91%), Postives = 703/1425 (49.33%), Query Frame = 1
Query:   43 MIFIFKDITFKFLLVLLSSLYCFSSTIWINDPSDESEICPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQLQ------------------------CINDNIFNKLGMLKYLLLEGNKIFWISEA-ISKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCASEDKANLENMCPSDKKV-CSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQIYNFQCN----------KEFESKYETHDEECVPKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLPPIYSIHEAPNLMKLDLSFNQIETLEPDVFTRSSK------------------------LREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQFHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLC-DGNASVYHSVDSCHETNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVLSFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYG-NYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGF--------RGKYCDIKFLYCN-DINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNSIYF-QKNSYIEISPPSRGFLMPRGNISLNFMTNSSSGLILYQGSEQTYLVVNLYQGHLRISYNLGDKVVGPEG-YSMGMVNDSRLHALHIEIVGENLTMYLNGRVHCTLKSKYLNSNRYMALDTGIFIGGAPHKILQSAIMKFHIET-----DSEFIGCMESLLINRKKIDFSKYL 4083
            M+   +   +   L  +S L     TI ++  +D ++ CP  C C   +V+C  +   +VP  +   T RL+L  N +  I K+DF  L +L+VLQL  N+IT+IE GAF +L  L +LRLNRN +   P+ LF   TKL +LDL +NQ+Q                        CI D  F  L  L+ L L  N I  +S A  + +  L+  R+H N L C+CH+AWL+ +L+Q+  +   G ++  C +P  L    ++ +   EF C    + +  +   S   + C + C CS++ IV C GK L+ IP +LP  + E+ L+ N I ++  G  S YK L++I L+ N + ++   AF GL  L S+ L  N +  I K  F GL SL  L L  N I CL  D  +    L+LL L +NKL ++ K TF     ++ L+L  NP  CDC + W+ DY++D    T  A C  PR L    +  ++   F+C+          +++ SK      +C   ++CP KC C    V+CSN  L  +P+ I   T  L +++N    L       + P+L K++LS N+I  +E   F  +S                         LR + L SNR+ C++N +F  L  + +L L +N ++ +   +F    +L  L L  N F+C+C L W+ +W R    V GN   P CQ+P  LK+ PI  +  + F C DGN     + +SC         CS                    T V CS+  L  +P  +PK+TTELYLD NH   +       L N K L L                     ++LS+N+L+CI   AF  L +L++L L  ND+S IP  AF DL +L+++ALG NP +CDC+++WL+ +    + + GI+ CA P  M  K +  +    F C+    D  I AKC+ C+  PCK++G C+    H Y+C CPY F G+NC+  I+AC   PC+NGG C   +  G N+ CVCP GF G  C IN+DDC  N C N +TC D  N+Y+C C   +        RG+ C+ K  +C  ++NPCQ+  +C L P   YKC C+ G+ G+ CE + DDCK N C++ +QC+D  N Y C+C  GY G FCE     P VL                          S C  HECLN   C+    +      C+C  GY G  C+ L S+ F  + SY+++  PS   L P+ NISL   T+  SG++LY+G +  ++ + LY+G LR+SY+ G     P   YS+  VND   HA+ +    + L++ ++G    ++ S  +N    + +D+ +++GG P +   S        +      + F GC+ +L IN K  D    L
Sbjct:    1 MVGALRPAAYSRSLATVSGLLVI--TILLSSGAD-AQPCPTQCSCTGTTVDCHGQGLRSVPRNIPRNTERLDLNANNLTKITKADFAGLRHLRVLQLMENKITTIERGAFQDLKELERLRLNRNNLAVFPELLFLGTTKLHRLDLSENQIQGVPRKAFRGAVEIKNLQLDYNHISCIEDGAFRALRDLEVLTLNNNNISRLSVASFNHMPKLRTFRLHSNNLQCDCHVAWLSEWLRQRPRL---GLYTQ-CMSPPHLRGHNVAEVQKKEFVCTDAVEGHQSSSSSSCSVLQCPESCTCSNN-IVDCRGKGLTEIPTNLPETITEIRLEQNAIKVIPAGAFSPYKRLRRIDLSNNQISELASDAFQGLRSLNSLVLYGNKITEISKGLFEGLFSLQLLLLNANKIACLRVDAFQDLHNLNLLSLYDNKLQTIAKGTFSSLRAIQTLHLAQNPFICDCHLKWLADYLQDNPIETSGARCTSPRRLANKRIGQIKSKKFRCSAREQYFIPGTEDYRSKL---GGDCFADLACPEKCRCEGTTVDCSNQKLTKIPDHIPQYTAELRLNNNEFTVLEATGIFKKLPHLRKINLSNNRITDIEEGTFEGASGVNELILTSNKLENIHHSMLKGLGGLRTLMLRSNRISCVSNSSFVGLSSVRLLSLYDNQVTSMTPGAFDTLHSLSTLNLLANPFNCNCHLAWLGDWLRRKRIVTGN---PRCQNPYFLKEIPIQDVAVQDFACEDGN-----NENSCSPVMRCPAECSC-----------------LDTVVRCSNKGLTTLPKGLPKETTELYLDGNHFTQVP----VELSNYKHLTL---------------------IILSYNRLRCIPVRAFDGLKSLRLLSLHGNDISLIPEGAFKDLSSLSHLALGANPLYCDCHMQWLSDWVKSGYKEPGIARCAGPGDMTDKLLLTTPSKKFTCTGPV-DLSIQAKCEPCLSNPCKNDGTCTNDPVHYYRCTCPYGFKGQNCEDPIHACISNPCQNGGTCHLKEGDGSNFWCVCPEGFEGDSCEINIDDCEDNDCENNSTCVDGINNYTCMCSPEYTAATNEVERGELCEEKLDFCAPELNPCQHDSKCILTPQG-YKCECTPGFVGEHCELDYDDCKDNKCQNGAQCIDAVNGYTCVCSEGYSGLFCEFAP--PMVLP-----------------------RTSPCDNHECLNGAQCVVVGTDPR----CQCLHGYEGERCETLVSVNFVNRESYLQL--PSN-LLSPQTNISLQIATDEDSGVLLYKG-DNDHIAMELYRGRLRVSYDTGS--YPPSAIYSVETVNDGSFHAVELVAAEQTLSLSIDGGPPKSIGS--INKQSTLNIDSPLYLGGMPERAFASGGFSALQHSPGGHNGTSFHGCLRNLYINGKLQDLGAGL 1325          

HSP 2 Score: 54.299 bits (129), Expect = 1.317e-6
Identity = 42/121 (34.71%), Postives = 60/121 (49.59%), Query Frame = 1
Query: 2899 QPCRN----GGICKNIDNYGNYECVCPVGFRGPKCTINVDD-CVGNLCVNGATCNDLNN-SYSCKCQSGFRGKYCDIKFLYCNDINPCQNS----GQCQLLPNNDYKCVCSKGWEGKQCER 3231
            QPC+      G C      G + C C  G+ G  C   V + C GN C+ G TC  +N+ SYSC+CQ GF G  CD +    + +N C  S    G+C++       C C+ G+ G+ C+R
Sbjct: 1351 QPCQRDTCVSGDCHPTGQRG-FTCTCHPGWTGTLCDQQVSNPCDGNKCIYG-TCVPINSYSYSCRCQPGFTGVLCDERDH--DTVNSCSLSHCKHGKCRVSGLGKAYCECNSGYTGEACDR 1467          
BLAST of slit1 vs. Ensembl Medaka
Match: slit1a (slit guidance ligand 1 [Source:NCBI gene;Acc:101164917])

HSP 1 Score: 679.478 bits (1752), Expect = 0.000e+0
Identity = 481/1583 (30.39%), Postives = 752/1583 (47.50%), Query Frame = 1
Query:  148 SEICPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQLQ------------------------CINDNIFNKLGMLKYLLLEGNKIFWIS-EAISKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCASEDKANLENMCPSDKKVCSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQIYNFQCNKEFE--SKYETHD----EECVPKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLPPIYSIHEAPNLMKLDLSFNQIETLEPDVFTRSSKLREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQ------------------------FHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCDGNASVYHSVDSCHETNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVLSFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICK-NIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYC-DIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNSIYF-QKNSYIEISPPSRGFLMPRGNISLNFMTNSSSGLILYQGSEQTYLVVNLYQGHLRISYNLGDKVVGPEGYSMGMVNDSRLHALHIEIVGENLTMYLNGRVHCTLKSKYLNSNRYMALDTGIFIGGAPHKILQSAIMKFHIETDSEFIGCMESLLINRKKIDFSKYLPKSINVHPGCGKLISTVVASDLKIPSIMXXXXXXXXXXXXXXXNNVCKRPGICKNQGV----CEVLPGSKKKFRCICKNGFVGRKCKKRKSM-------------------------CHGTFTESHIVDPDSQGTCKSIERFQYRICTG--TCSTSRMQSDVIKNFYHKPIDPSSSRCCQPTKYSTHEIQFKCENGRVYRRAANLPKNCKCVA 4629
            +  CP  C C   +V+C       +P  +   T RL L GN +  I KSDF  L  ++VLQL  N+IT +E GAFD++  L +LRLNRN +Q LP+ LF K   L +LDL +N +Q                        CI D  F  +  L+ L L  N I  I   + + +  L+  R+H N LNC+CHLAWL  +L+Q+  I         C+ P  L  + ++ +  +EF C+   +++    C      C   C CS++ IV C GK L+ IP +LP N+ E+ L+ N I  +  G  S YK L++I L+ N + +I P AF GL  L S+ L  N +  + K  F GL +L  L L  N I C+  +T +  + L LL L +NK+ +L K TF     ++ L+L  NP  CDC + W+ DY++     T  A C  PR L    +  ++   F+C+      S   T D    + C     CP KC C S+VV+CSN  L  +PE I  ST  L +++N +  L    +      L K++LS N+I  +E   F  +S + E+ L +N++  + +  F+ L+ L +L L  N ISC+  DSF    N++ L L  NQ                        F+CDC+L W+ +W R+   V GN   P CQ P  LK+ P+  +    F CD          SC       + C+                    T V CS+  L  +P  IP++ TELYLD N   S+          L+ + LS N I  L  +    +  L TL+LS+N L+CI + AF  LH+L++L L  N++S +P   FND+ +L+++A+G NP HCDC ++WL+ +    + + GI+ CA P+ ++ K +  +    F C  +  D  + +KC+ C+  PC ++G C       Y+C CP  F GKNC+  +NAC   PC NGG C+ + DN G Y C C +GF GP C IN DDC  N C NGATC D  N+Y+C C   + G+ C +++ +     +PCQ+   C L+ ++  KCVCS G+ G  C  + +DC+ + C++ +QCVD  + Y C+C  G+ G+ CE+P   P+ L                          S+C   +C N+  C+     +  ++ C+C P + G  C+ L S+ F  ++SY+ +S        P+ NI+L   T   +G++LY G +  ++ V L+QGH+++SY+ G +  G   YS   +ND + H + +    + + + ++G +  T+ S    + R +  +  +++GG P  +       + I+  S F GC+++L IN +  DF+K   K   V PGC           +  P   Q                 C +P +   QG      + +P   + +RC C+ G+ G  C ++  +                         C   +T   + D +S+   + +  F Y++  G   C T+RM S     +       S+  CC   +    +  F+C +G  +         C CV 
Sbjct:   33 ASACPALCTCSGVTVDCHGLGLKTMPRNIPRNTERLELNGNNLTRITKSDFTGLKYVRVLQLMENQITVVERGAFDDMKELERLRLNRNQLQQLPELLFQKNPALSRLDLSENFIQSIPRKAFRGATDIKNLQLDKNHISCIEDGAFRAMRGLEVLTLNNNNISSIPVSSFNHMPKLRTFRLHSNNLNCDCHLAWLAQWLRQRPTI----GLFTQCTVPSELKGLNVAEVQKHEFTCSGHQESSSLQPCSIGGGSCPAMCTCSNN-IVDCRGKGLTAIPANLPDNMAEIRLEQNGIKSVPPGAFSSYKKLRRIDLSNNQISEIAPDAFQGLRSLNSLVLYGNKITDLPKGVFDGLYALQLLLLNANKIHCIRANTFQDLQNLSLLSLYDNKIQTLAKGTFTSLRAIQTLHLAQNPFICDCNLKWLADYLRSNPIETSGARCASPRRLANKRIGQIKSKKFRCSGSILTFSPLGTEDVRLNDACNSDPVCPPKCRCESNVVDCSNLKLTKIPEHIPSSTSELRLNNNEITSLDANGAFKNLAQLKKINLSNNKITEIEDGTFEGASAVIELHLTANQIDSVRSGMFRGLEGLRMLMLRNNKISCVHNDSFTGLHNVRLLSLYDNQLTTITPGAFDTLQTLSTLNLLANSFNCDCRLAWLGDWLRSRKIVTGN---PRCQRPAFLKEIPLQDVALPDFRCDEG----QEESSCIPRPQCPSECTC-----------------LETVVRCSNKHLHSLPRGIPRNVTELYLDGNQF-SIVPKELSTFKYLQLVDLSNNKINSLTNSSFANMSQLTTLILSYNSLRCIPKMAFSGLHSLRLLSLHGNEISELPDGIFNDVASLSHLAIGANPLHCDCRLRWLSDWVKTGYKEPGIARCAGPQGLEGKLLLTTPAKKFECHGDV-DIAVLSKCNPCLSNPCMNHGICHNDPVEIYRCSCPPGFKGKNCESGLNACVSNPCANGGTCRVHEDNEGQYSCTCALGFEGPTCKINTDDCEDNDCENGATCVDGINNYTCFCPPYYTGEMCEEMEDVCAPGRSPCQHQSTC-LITSSGPKCVCSPGYVGDDCSIDYNDCEEHRCQNGAQCVDELSGYSCICPKGFSGQLCEVP---PSPL--------------------------SLCELADCQNDAPCV----ERGGRALCQCPPEFGGPRCEKLVSVNFIDRDSYLLLSDLKN---WPQANITLQVSTAEDNGILLYNG-DNDHIAVELFQGHVKVSYDPGSQ-PGHAIYSTETINDGQFHTVELVTFDQMVNLSIDGGLPTTMDS--FGAVRPLNREAPLYVGGMPVDVHSGTFRLWQIQNSSSFHGCIQNLYINNELQDFTKTQMKP-GVVPGCEPCKKLYCVHGICQPDGAQGPVCHCQPGWIGAH---CDQPALNPCQGSKCVHGKCIPLDAQSYRCECQEGYRGALCNQQGELFNPCRRLSCKHGRCQISDAGSAYCHCESGYT-GELCDAESECRGEPVRDF-YQVQRGYAICQTTRMVS-----WVECSGACSTGACCGSQRMKRRKYTFECSDGTSFSEEVEKTIKCGCVG 1532          

HSP 2 Score: 188.348 bits (477), Expect = 1.806e-47
Identity = 135/453 (29.80%), Postives = 226/453 (49.89%), Query Frame = 1
Query:  121 IWINDPSDESEICPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLK-LNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQLQCINDNIFNKLGMLKYLLLEGNKIFWISE-AISKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCASEDKANLENMCPSDKKVCSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQIYNFQCNKEFE 1473
            + +ND  +   +CP  C+CE+  V+C +     +P  +   T+ L L  N I  +  +   K L  LK + LSNN+IT IE G F+    +I+L L  N I  +   +F  L  L+ L LR+N++ C++++ F  L  ++ L L  N++  I+  A   L+ L  L +  N  NC+C LAWL  +L+ +  +      +  C  P  L  IP+  ++  +F+C   D+   E+ C   +  C  +C C  + +V+C  K L ++P+ +P NV EL L  N  +I+ +  LS +K+L+ + L+ N +  +   +F  ++QLT++ L+ N+L+ I K AF GL SL  L L  N+I  LP                           F    +L +L +  NPL+CDCR+ W+ D++K       +A C  P+ L+G  L       F+C+ + +
Sbjct:  504 VRLNDACNSDPVCPPKCRCESNVVDCSNLKLTKIPEHIPSSTSELRLNNNEITSLDANGAFKNLAQLKKINLSNNKITEIEDGTFEGASAVIELHLTANQIDSVRSGMFRGLEGLRMLMLRNNKISCVHNDSFTGLHNVRLLSLYDNQLTTITPGAFDTLQTLSTLNLLANSFNCDCRLAWLGDWLRSRKIVTG----NPRCQRPAFLKEIPLQDVALPDFRC---DEGQEESSC-IPRPQCPSECTC-LETVVRCSNKHLHSLPRGIPRNVTELYLDGNQFSIVPK-ELSTFKYLQLVDLSNNKINSLTNSSFANMSQLTTLILSYNSLRCIPKMAFSGLHSLRLLSLHGNEISELPDG------------------------IFNDVASLSHLAIGANPLHCDCRLRWLSDWVKTGYKEPGIARCAGPQGLEGKLLLTTPAKKFECHGDVD 922          
BLAST of slit1 vs. Planmine SMEST
Match: SMESG000068721.1 (SMESG000068721.1)

HSP 1 Score: 2980.27 bits (7725), Expect = 0.000e+0
Identity = 1531/1534 (99.80%), Postives = 1532/1534 (99.87%), Query Frame = 1
Query:   43 MIFIFKDITFKFLLVLLSSLYCFSSTIWINDPSDESEICPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQLQCINDNIFNKLGMLKYLLLEGNKIFWISEAISKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCASEDKANLENMCPSDKKVCSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQIYNFQCNKEFESKYETHDEECVPKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLPPIYSIHEAPNLMKLDLSFNQIETLEPDVFTRSSKLREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQFHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCDGNASVYHSVDSCHETNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVLSFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNSIYFQKNSYIEISPPSRGFLMPRGNISLNFMTNSSSGLILYQGSEQTYLVVNLYQGHLRISYNLGDKVVGPEGYSMGMVNDSRLHALHIEIVGENLTMYLNGRVHCTLKSKYLNSNRYMALDTGIFIGGAPHKILQSAIMKFHIETDSEFIGCMESLLINRKKIDFSKYLPKSINVHPGCGKLISTVVASDLKIPSIMXXXXXXXXXXXXXXXNNVCKRPGICKNQGVCEVLPGSKKKFRCICKNGFVGRKCKKRKSMCHGTFTESHIVDPDSQGTCKSIERFQYRICTGTCSTSRMQSDVIKNFYHKPIDPSSSRCCQPTKYSTHEIQFKCENGRVYRRAANLPKNCKCVANCLND 4644
            MIFIFKDITFKFLLVLLSSLYCFSSTIWINDPSDESEICPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDNLVNLIKLRLNRNLIQYLPDDLFSKLTKLQKLDLRDNQLQCINDNIFNKLGMLKYLLLEGNKIFWISEAISKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCASEDKANLENMCPSDKKVCSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQIYNFQCNKEFESKYETHDEECVPKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKL PIYSIHEAPNLMKLDLSFNQIETLEPDVFTRSSKLREI+LNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFKNNLNLKYLMLNKNQFHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCDGNASV HSVDSCHETNSVKTCCSIGNEECTKSKKCPSKCKCESTKVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVLSFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNSIYFQKNSYIEISPPSRGFLMPRGNISLNFMTNSSSGLILYQGSEQTYLVVNLYQGHLRISYNLGDKVVGPEGYSMGMVNDSRLHALHIEIVGENLTMYLNGRVHCTLKSKYLNSNRYMALDTGIFIGGAPHKILQSAIMKFHIETDSEFIGCMESLLINRKKIDFSKYLPKSINVHPGCGKLISTVVASDLKIPSIMQPIKQILTQTTKKKPNNVCKRPGICKNQGVCEVLPGSKKKFRCICKNGFVGRKCKKRKSMCHGTFTESHIVDPDSQGTCKSIERFQYRICTGTCSTSRMQSDVIKNFYHKPIDPSSSRCCQPTKYSTHEIQFKCENGRVYRRAANLPKNCKCVANCLND
Sbjct:    1 MIFIFKDITFKFLLVLLSSLYCFSSTIWINDPSDESEICPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDNLVNLIKLRLNRNLIQYLPDDLFSKLTKLQKLDLRDNQLQCINDNIFNKLGMLKYLLLEGNKIFWISEAISKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCASEDKANLENMCPSDKKVCSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQIYNFQCNKEFESKYETHDEECVPKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLTPIYSIHEAPNLMKLDLSFNQIETLEPDVFTRSSKLREINLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFKNNLNLKYLMLNKNQFHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCDGNASVDHSVDSCHETNSVKTCCSIGNEECTKSKKCPSKCKCESTKVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVLSFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNSIYFQKNSYIEISPPSRGFLMPRGNISLNFMTNSSSGLILYQGSEQTYLVVNLYQGHLRISYNLGDKVVGPEGYSMGMVNDSRLHALHIEIVGENLTMYLNGRVHCTLKSKYLNSNRYMALDTGIFIGGAPHKILQSAIMKFHIETDSEFIGCMESLLINRKKIDFSKYLPKSINVHPGCGKLISTVVASDLKIPSIMQPIKQILTQTTKKKPNNVCKRPGICKNQGVCEVLPGSKKKFRCICKNGFVGRKCKKRKSMCHGTFTESHIVDPDSQGTCKSIERFQYRICTGTCSTSRMQSDVIKNFYHKPIDPSSSRCCQPTKYSTHEIQFKCENGRVYRRAANLPKNCKCVANCLND 1534          
BLAST of slit1 vs. Planmine SMEST
Match: SMESG000063217.1 (SMESG000063217.1)

HSP 1 Score: 135.191 bits (339), Expect = 2.602e-31
Identity = 84/246 (34.15%), Postives = 117/246 (47.56%), Query Frame = 1
Query: 2722 ICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQN--------------------------------------SGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEI 3345
            IC    E        D+C K  C + G C  +     +C+C   F G NC+  +N C  +PC+N   C +++N  +Y C CP G  G  C IN+D+C  N C+NGA C D    ++C+C  GF G  C+ K   C  + PC+                                       +GQC  + NN+ KC+C  G+EG+ CE   ++C  N C+H SQC DL NDY C+C PG+ GK CEI
Sbjct:  316 ICQRGFEGKRCETSIDNCQKISCFNGGSCINMIEGG-KCNCLPGFEGNNCEINVNECENKPCQNNAKCIDLNN--DYTCDCPEGLTGKNCEINIDECTPNPCLNGARCEDRVGGFTCQCLQGFVGLRCETKSDICKQLTPCKTEEICLNVNNTRSGYKCICPDGVQNCKKELSKCKNIVCVNGQCVDV-NNEEKCICIPGFEGRTCEIEINECFNNPCQHGSQCQDLFNDYLCICLPGFKGKKCEI 557          

HSP 2 Score: 126.716 bits (317), Expect = 9.637e-29
Identity = 73/186 (39.25%), Postives = 103/186 (55.38%), Query Frame = 1
Query: 2788 CKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEI 3345
            C++ G C + T  +YQC C   F+G+ C+  I  C    C+NGG C  +D  G+Y CVCP  + G  C      C    C NG  C D +  Y C C + + GK CD     C  INPC+N+G C+ +   DYKC+C +G+EGK+CE + D+C+  SC +   C+++    KC C PG+ G  CEI
Sbjct:  185 CENGGTC-INTVGSYQCKCKAGFNGERCENDIVDCTNDTCKNGGTC--LDKIGSYRCVCPNNYFGTNCENKRTLCELKPCKNGGICRDFDEDYQCICGNQWTGKNCDDVKNPC-KINPCKNNGFCESVNYQDYKCICQRGFEGKRCETSIDNCQKISCFNGGSCINMIEGGKCNCLPGFEGNNCEI 366          

HSP 3 Score: 120.939 bits (302), Expect = 5.523e-27
Identity = 87/298 (29.19%), Postives = 144/298 (48.32%), Query Frame = 1
Query: 2767 DSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVG---FRGPKCTINVDDC---------------------VGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAY-------HECLNNGICINGDQNKNQKSY-CKCKPGYSGHLCQ 3564
            + C   PC  NG+C +   + ++C C  P+ G  C  +++ C    C N  IC   + Y +Y C C      + G KC  +VD+C                     +G  C+N   C + N S+ CKC S + G+ C+     C + +PC+N   C  L +  ++C C  G+ G++CE   ++C++N C ++++C+D  NDY+C+C+ G+ GK CEI     T    L   + +++    +E   NE    + C +       + CLN G CIN   N+N   Y C C+ G++G  C+
Sbjct:  560 NECAHNPCGLNGQC-IDEVYGFRCQCMLPYSGPTCANKLDPCNPNACYNDAICIPGNKYDSYHCNCSTALGRYEGSKCEKDVDECNLDRTQLALYGLALLSMSNSQIG-PCLNDNQCINTNGSFICKCSSQYEGRQCEKPLNNCRN-DPCRNGATCDNLADG-FRCRCVAGFTGERCEIEINECQHNPCSNDAKCIDHINDYQCICQSGFSGKNCEINDNDCTESSCLGHGQCVDKVN-GFECKCNENYIGTNCEHLKRPCDSNPCLNKGSCINRKVNQNTPGYLCNCRRGFTGPRCE 852          

HSP 4 Score: 118.627 bits (296), Expect = 2.933e-26
Identity = 87/286 (30.42%), Postives = 123/286 (43.01%), Query Frame = 1
Query: 2725 CSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQC------QLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQ 3564
            CS + E        ++C   PC++   C  +    ++C C   F G+ C+  IN C   PC N   C  ID+  +Y+C+C  GF G  C IN +DC  + C+    C D  N + CKC   + G  C+     C D NPC N G C      Q  P   Y C C +G+ G +CE   D C+ N C     C    + +KC C   YIG+ CEI             ++         E  +NE +   +C +H     G C N  +    K  C C+ GY G  C+
Sbjct:  685 CSSQYEGRQCEKPLNNCRNDPCRNGATCDNLA-DGFRCRCVAGFTGERCEIEINECQHNPCSNDAKC--IDHINDYQCICQSGFSGKNCEINDNDCTESSCLGHGQCVDKVNGFECKCNENYIGTNCEHLKRPC-DSNPCLNKGSCINRKVNQNTPG--YLCNCRRGFTGPRCEIKMDWCRGNPCGDFGVCETDLDSFKCKCNSEYIGERCEI-------------KKTETCKSYAVEKFKNESLINKICKHH-----GNCFNDPETGGFK--CSCQSGYGGSFCE 944          

HSP 5 Score: 117.472 bits (293), Expect = 6.161e-26
Identity = 93/314 (29.62%), Postives = 131/314 (41.72%), Query Frame = 1
Query: 2773 CMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQP-------------------------------------------CRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDL-HNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENL------ENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQ 3564
            C   PC++ G C  +  H Y+C CP  + G NC+   N+C  Q                                            C NGG C  I+  G+Y+C C  GF G +C  ++ DC  + C NG TC D   SY C C + + G  C+ K   C ++ PC+N G C+   + DY+C+C   W GK C+   + CK N CK+   C  + + DYKC+C+ G+ GK CE        +        IN  E    N        N  I+ + C    C NN  CI    + N    C C  G +G  C+
Sbjct:   99 CASSPCRNGGICKSLEIHKYECQCPKNYLGMNCE-LPNSCGSQNPCGVNGKCILANDGEVKCHCNKGYTGDTCSNDIDECASYETPLCENGGTC--INTVGSYQCKCKAGFNGERCENDIVDCTNDTCKNGGTCLDKIGSYRCVCPNNYFGTNCENKRTLC-ELKPCKNGGICRDF-DEDYQCICGNQWTGKNCDDVKNPCKINPCKNNGFCESVNYQDYKCICQRGFEGKRCETSIDNCQKISCFNGGSCINMIEGGKCNCLPGFEGNNCEINVNECENKPCQNNAKCI----DLNNDYTCDCPEGLTGKNCE 403          

HSP 6 Score: 115.161 bits (287), Expect = 3.589e-25
Identity = 90/302 (29.80%), Postives = 136/302 (45.03%), Query Frame = 1
Query: 2767 DSCMKRPCKHNGKC-SLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHES----------------QCVDLHNDYKCMCRPGYIGKFCE--------------IPAWKPTVLKTLISR---RDINQSEMTYENLENEV--ISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQ 3564
            +S + + CKH+G C +      ++C C   + G  C+  I+ C  QPC NGG C   D  G Y C C  GF+G  C +N ++C  N C N   C D  N++SC C  G  G++C+    YC  +  CQN G C+ +   D++C C KG+ GK CE + ++CKYN   + +                +C+D    + C C+  + GK+CE              IP +   +          R+ N S +       E   I+   C  + C NNGIC   + +      C+C PG  G  C+
Sbjct:  908 ESLINKICKHHGNCFNDPETGGFKCSCQSGYGGSFCEVNIDDCASQPCINGGECG--DGTGMYFCKCQPGFQGVNCQVNTNECYSNSC-NTGKCIDQINNFSCVCPRGVSGRFCETIVDYCKKME-CQNGGICKNVIG-DFECNCPKGFAGKLCETDINECKYNIAGNATNLISNPHGPCSPTGTLECLDRPGTFHCSCKKYFTGKYCETRIDYCEKFRKSGNIPCFNNGICLNKYQNEVGRNYNFSCICPNGYGGERCKINSKKCQLNVCKNNGICRPFEIDDRMSFKCECPPGVGGLFCE 1204          

HSP 7 Score: 111.309 bits (277), Expect = 4.656e-24
Identity = 86/290 (29.66%), Postives = 128/290 (44.14%), Query Frame = 1
Query: 2800 GKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGAT----------------CNDLNNSYSCKCQSGFRGKYCDIKFLYCNDIN-----PCQNSGQC------QLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQC----VDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNS 3576
            GKC +   +N+ C CP    G+ C+  ++ C    C+NGGICKN+   G++EC CP GF G  C  ++++C  N+  N                   C D   ++ C C+  F GKYC+ +  YC         PC N+G C      ++  N ++ C+C  G+ G++C+ N+  C+ N CK+   C    +D    +KC C PG  G FCEI                         +  NE +S       +C NNG C+NG      K+ C C  G  G  C+   S
Sbjct:  996 GKC-IDQINNFSCVCPRGVSGRFCETIVDYCKKMECQNGGICKNV--IGDFECNCPKGFAGKLCETDINECKYNIAGNATNLISNPHGPCSPTGTLECLDRPGTFHCSCKKYFTGKYCETRIDYCEKFRKSGNIPCFNNGICLNKYQNEVGRNYNFSCICPNGYGGERCKINSKKCQLNVCKNNGICRPFEIDDRMSFKCECPPGVGGLFCEI-------------------------DYVNECLST------KCYNNGSCVNGF----GKAICHCNKGSCGEKCEITGS 1247          

HSP 8 Score: 105.531 bits (262), Expect = 3.232e-22
Identity = 94/313 (30.03%), Postives = 134/313 (42.81%), Query Frame = 1
Query: 2761 KCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCD-KRINAC-------------FGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCE--IPAWKPTV---LKTLISRRDINQSEM-TYENLENEVISESVCAYH----------------------ECLNNGICINGDQNKNQKSY---CKCKPGYSGHLCQ 3564
            K D C   PC   G C      +++C C   + G+ C+ K+   C               + C++ G C N    G ++C C  G+ G  C +N+DDC    C+NG  C D    Y CKCQ GF+G  C +    C   N C N+G+C +   N++ CVC +G  G+ CE   D CK   C++   C ++  D++C C  G+ GK CE  I   K  +      LIS      S   T E L+        C  +                       C NNGIC+N  QN+  ++Y   C C  GY G  C+
Sbjct:  854 KMDWCRGNPCGDFGVCE-TDLDSFKCKCNSEYIGERCEIKKTETCKSYAVEKFKNESLINKICKHHGNCFNDPETGGFKCSCQSGYGGSFCEVNIDDCASQPCINGGECGDGTGMYFCKCQPGFQGVNCQVNTNECYS-NSC-NTGKC-IDQINNFSCVCPRGVSGRFCETIVDYCKKMECQNGGICKNVIGDFECNCPKGFAGKLCETDINECKYNIAGNATNLISNPHGPCSPTGTLECLDRPGTFHCSCKKYFTGKYCETRIDYCEKFRKSGNIPCFNNGICLNKYQNEVGRNYNFSCICPNGYGGERCK 1162          

HSP 9 Score: 98.9821 bits (245), Expect = 3.009e-20
Identity = 78/258 (30.23%), Postives = 121/258 (46.90%), Query Frame = 1
Query: 2773 CMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLN-NSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDC-KYNS--CKHESQCVDLHNDYKCMCRPGYIGKFCE---------IPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQN 3507
            C    CK+   C    +  Y C+C Y + G  C+++ N C   PC+NGG CK I   GNY C C   + G  C  +  +C  + C NG  C  L  + Y C+C   + G  C++    C   NPC  +G+C L  + + KC C+KG+ G  C  + D+C  Y +  C++   C++    Y+C C+ G+ G+ CE               T L  + S R +  +     N EN+    ++C    C N GIC + D++
Sbjct:   24 CENHKCKNGATCQSDINGKYVCECAYGYKGTYCEQKSN-CPITPCQNGGTCKLI--AGNYVCQCLNEWVGEFCEFS-SNCASSPCRNGGICKSLEIHKYECQCPKNYLGMNCELPN-SCGSQNPCGVNGKCILANDGEVKCHCNKGYTGDTCSNDIDECASYETPLCENGGTCINTVGSYQCKCKAGFNGERCENDIVDCTNDTCKNGGTCLDKIGSYRCVCPNNYFGTNCENK---RTLCELKPCKNGGICRDFDED 273          

HSP 10 Score: 91.2781 bits (225), Expect = 6.180e-18
Identity = 81/267 (30.34%), Postives = 123/267 (46.07%), Query Frame = 1
Query: 2785 PCKHNGKCSLI--THHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCV--DLHNDYKCMCRPG---YIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQ 3564
            PCK    C  +  T   Y+C CP     +NC K ++ C    C NG  C +++N    +C+C  GF G  C I +++C  N C +G+ C DL N Y C C  GF+GK C+I    C   NPC  +GQC +     ++C C   + G  C    D C  N+C +++ C+  + ++ Y C C      Y G  CE            +   +++++++    L   ++S S      CLN+  CI    N N    CKC   Y G  C+
Sbjct:  452 PCKTEEICLNVNNTRSGYKCICPDGV--QNCKKELSKCKNIVCVNGQ-CVDVNN--EEKCICIPGFEGRTCEIEINECFNNPCQHGSQCQDLFNDYLCICLPGFKGKKCEININECAH-NPCGLNGQC-IDEVYGFRCQCMLPYSGPTCANKLDPCNPNACYNDAICIPGNKYDSYHCNCSTALGRYEGSKCE----------KDVDECNLDRTQLALYGL--ALLSMSNSQIGPCLNDNQCI----NTNGSFICKCSSQYEGRQCE 695          

HSP 11 Score: 65.4698 bits (158), Expect = 4.358e-10
Identity = 52/158 (32.91%), Postives = 73/158 (46.20%), Query Frame = 1
Query: 2830 YQCDCPYPFHGKNCDKRINAC--FGQ----PCRNGGICKN-----IDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCN----DLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNS 3258
            + C C   F GK C+ RI+ C  F +    PC N GIC N     +    N+ C+CP G+ G +C IN   C  N+C N   C     D   S+ C+C  G  G +C+I ++       C N+G C +       C C+KG  G++CE     C   S
Sbjct: 1097 FHCSCKKYFTGKYCETRIDYCEKFRKSGNIPCFNNGICLNKYQNEVGRNYNFSCICPNGYGGERCKINSKKCQLNVCKNNGICRPFEIDDRMSFKCECPPGVGGLFCEIDYVNECLSTKCYNNGSC-VNGFGKAICHCNKGSCGEKCEITGSACNLAS 1253          

HSP 12 Score: 56.6102 bits (135), Expect = 1.980e-7
Identity = 47/158 (29.75%), Postives = 71/158 (44.94%), Query Frame = 1
Query: 2707 KPTDFICS--EETEDAYITAKCDSCMKR------PCKHNGKC------SLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICK--NIDNYGNYECVCPVGFRGPKCTIN-VDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCN 3129
            +P  F CS  +     Y   + D C K       PC +NG C       +  ++N+ C CP  + G+ C      C    C+N GIC+   ID+  +++C CP G  G  C I+ V++C+   C N  +C +      C C  G  G+ C+I    CN
Sbjct: 1093 RPGTFHCSCKKYFTGKYCETRIDYCEKFRKSGNIPCFNNGICLNKYQNEVGRNYNFSCICPNGYGGERCKINSKKCQLNVCKNNGICRPFEIDDRMSFKCECPPGVGGLFCEIDYVNECLSTKCYNNGSCVNGFGKAICHCNKGSCGEKCEITGSACN 1250          
BLAST of slit1 vs. Planmine SMEST
Match: SMESG000027402.1 (SMESG000027402.1)

HSP 1 Score: 133.265 bits (334), Expect = 7.828e-31
Identity = 105/355 (29.58%), Postives = 152/355 (42.82%), Query Frame = 1
Query: 2722 ICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDI----------------------NPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENE-----------VISESVCAYHECLNNGIC-----INGDQNKNQKSYCKCKPGYSGHLCQYLNSIYFQKNSYIEISPPSRGFLMPRGNISLNFMTN 3672
            +C       Y   + + C+  PCK+NG C+  + ++Y+C+C   F GKNC+  I AC  QPC+NGG+C+ +D    Y C C  G+ G  C I    C  N C+NGA C  +N  + C C   F GK C+    YC +                       N C   G C       + C C  G+EG  CE + DDCK N CK+   C+D  N+++C+C  G+ GK CE           +     +  S+  Y +   E           +   S+C  + CLN G C     +NG Q       C C  G+ G +CQ+L         +I+  P  R  L     + +N   N
Sbjct:  439 LCQPGYTGKYCDKEINECVPNPCKNNGVCT-DSINDYKCNCSTSFKGKNCELNI-ACQNQPCKNGGVCQEVDT--GYTCKCKTGYTGTNCEIYKTGCSPNPCLNGAYCYTINQDFYCHCNKEFWGKRCEHSRPYCGNESCESAYDACSMPQMLNSSGIKHNYCGKYGTCLSHEKEKFICRCMPGFEGDSCEIDIDDCKTNPCKNGGICIDSINNFECICDNGFFGKTCEERTDYCIKSPCMNYGVCLRHSDELYNDFICECKNNWGGRLCHLPKHSICDSNPCLNGGSCSRLSTLNGFQ-------CHCNQGWIGTVCQFL---------HIDFQPCRRKNLCKNEGVCINVGYN 773          

HSP 2 Score: 112.079 bits (279), Expect = 2.340e-24
Identity = 70/201 (34.83%), Postives = 106/201 (52.74%), Query Frame = 1
Query: 2767 DSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDN--YGNYECVCPVGFRGPKCTINVDD-CVGNLCVNGATCNDLN--NSYSCKCQSGFRGKYCD---IKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEI 3345
            D C   PCK+ G C + + +N++C C   F GK C++R + C   PC N G+C    +  Y ++ C C   + G  C +     C  N C+NG +C+ L+  N + C C  G+ G  C    I F  C   N C+N G C  +  ND+ C C  G+EGK C+ NT++C  N C +  +C+D  N Y+C+C  G+ G  C++
Sbjct:  629 DDCKTNPCKNGGIC-IDSINNFECICDNGFFGKTCEERTDYCIKSPCMNYGVCLRHSDELYNDFICECKNNWGGRLCHLPKHSICDSNPCLNGGSCSRLSTLNGFQCHCNQGWIGTVCQFLHIDFQPCRRKNLCKNEGVCINVGYNDFTCHCKPGFEGKHCDINTNECNPNPCFNGGKCIDKLNSYRCICAKGFYGSNCQL 828          

HSP 3 Score: 111.694 bits (278), Expect = 2.680e-24
Identity = 101/319 (31.66%), Postives = 135/319 (42.32%), Query Frame = 1
Query: 2764 CDSCMKRP-CKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACF-GQPCRNGGICKNI-DNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQC----------------------------------------QLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEI-------PAWKPTVLKTLISRRDIN-QSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQY 3567
            CD CMK P CKH G C       + C C   + G+ CD+ +N C    PC+NGGIC+N+      Y+C+CP GF G  C I  D C  N C  G TC      + C+C   + GK CD     C D N CQN GQC                                        Q     DYKC+C  G+ GK C++  ++C  N CK+   C D  NDYKC C   + GK CE+       P     V + + +      ++  T  N E   I ++ C+ + CLN   C       NQ  YC C   + G  C++
Sbjct:  261 CDECMKYPGCKH-GTC---VDSPWTCSCENNWGGQFCDQSLNYCAENNPCKNGGICENLLSEVQPYKCICPSGFEGRDCEI--DPCFPNPCKKG-TCMKSKGEFYCQCIERWTGKLCDQDTSIC-DQNSCQNGGQCGKDENGNRRCICQQGYKGSECHLAAPCENSQCKHALDCQIQSTALGDYKCLCQPGYTGKYCDKEINECVPNPCKNNGVCTDSINDYKCNCSTSFKGKNCELNIACQNQPCKNGGVCQEVDTGYTCKCKTGYTGTNCE---IYKTGCSPNPCLNGAYCY----TINQDFYCHCNKEFWGKRCEH 564          

HSP 4 Score: 110.923 bits (276), Expect = 4.650e-24
Identity = 86/280 (30.71%), Postives = 132/280 (47.14%), Query Frame = 1
Query: 2788 CKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATC----NDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPN-NDYKCVCSKGWEGKQCERNTDDC----KYNSCKHESQCVDL-HNDYKCMCRPGYIGKFCEI--------PAWKP-TVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYL 3570
            C   G C       + C C   F G +C+  I+ C   PC+NGGIC  ID+  N+EC+C  GF G  C    D C+ + C+N   C    ++L N + C+C++ + G+ C +      D NPC N G C  L   N ++C C++GW G  C+    D     + N CK+E  C+++ +ND+ C C+PG+ GK C+I        P +     +  L S R I        N +   ++   CA   C    IC++    +     C+C  G  G LC ++
Sbjct:  597 CGKYGTCLSHEKEKFICRCMPGFEGDSCEIDIDDCKTNPCKNGGIC--IDSINNFECICDNGFFGKTCEERTDYCIKSPCMNYGVCLRHSDELYNDFICECKNNWGGRLCHLPKHSICDSNPCLNGGSCSRLSTLNGFQCHCNQGWIGTVCQFLHIDFQPCRRKNLCKNEGVCINVGYNDFTCHCKPGFEGKHCDINTNECNPNPCFNGGKCIDKLNSYRCICAKGFYGSNCQ---LNYDECASFPCAYGAICVD----RIDDFECRCPEGRDGKLCDHV 867          

HSP 5 Score: 105.145 bits (261), Expect = 3.302e-22
Identity = 71/201 (35.32%), Postives = 107/201 (53.23%), Query Frame = 1
Query: 2767 DSCMKRPCKHNGKC---SLITHHNYQCDCPYPFHGKNCD-KRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCT---INVDDCV-GNLCVNGATCNDLN-NSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCE 3342
            D C+K PC + G C   S   ++++ C+C   + G+ C   + + C   PC NGG C  +     ++C C  G+ G  C    I+   C   NLC N   C ++  N ++C C+ GF GK+CDI    CN  NPC N G+C +   N Y+C+C+KG+ G  C+ N D+C    C + + CVD  +D++C C  G  GK C+
Sbjct:  667 DYCIKSPCMNYGVCLRHSDELYNDFICECKNNWGGRLCHLPKHSICDSNPCLNGGSCSRLSTLNGFQCHCNQGWIGTVCQFLHIDFQPCRRKNLCKNEGVCINVGYNDFTCHCKPGFEGKHCDINTNECNP-NPCFNGGKC-IDKLNSYRCICAKGFYGSNCQLNYDECASFPCAYGAICVDRIDDFECRCPEGRDGKLCD 865          

HSP 6 Score: 88.9669 bits (219), Expect = 2.819e-17
Identity = 92/358 (25.70%), Postives = 148/358 (41.34%), Query Frame = 1
Query: 2626 FLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKCD---SCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKC-----------------------TINVDDCVGNLCVNGATC-NDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCV----DLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMT----YENLENEVISESVCAY-----------HECLNNGICINGDQNKNQKSYCKCKPGYSGHLC 3561
            +  ++ D  I+ C  P   K   +      D+ C+  T  ++    C+   +C  +PCK+ G C  +    Y C C   + G NC+     C   PC NG  C  I+   ++ C C   F G +C                        +N      N C    TC +     + C+C  GF G  C+I    C   NPC+N G C +   N+++C+C  G+ GK CE  TD C  + C +   C+    +L+ND+ C C+  + G+ C +P  K ++  +       + S ++    ++   N+    +VC +           + C N G+CIN   N      C CKPG+ G  C
Sbjct:  444 YTGKYCDKEINECV-PNPCKNNGVCTDSINDYKCNCST--SFKGKNCELNIACQNQPCKNGGVCQEVDT-GYTCKCKTGYTGTNCEIYKTGCSPNPCLNGAYCYTINQ--DFYCHCNKEFWGKRCEHSRPYCGNESCESAYDACSMPQMLNSSGIKHNYCGKYGTCLSHEKEKFICRCMPGFEGDSCEIDIDDCK-TNPCKNGGIC-IDSINNFECICDNGFFGKTCEERTDYCIKSPCMNYGVCLRHSDELYNDFICECKNNWGGRLCHLP--KHSICDSNPCLNGGSCSRLSTLNGFQCHCNQGWIGTVCQFLHIDFQPCRRKNLCKNEGVCINVGYNDFT---CHCKPGFEGKHC 788          
BLAST of slit1 vs. Planmine SMEST
Match: SMESG000027402.1 (SMESG000027402.1)

HSP 1 Score: 132.88 bits (333), Expect = 8.619e-31
Identity = 105/355 (29.58%), Postives = 152/355 (42.82%), Query Frame = 1
Query: 2722 ICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDI----------------------NPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENE-----------VISESVCAYHECLNNGIC-----INGDQNKNQKSYCKCKPGYSGHLCQYLNSIYFQKNSYIEISPPSRGFLMPRGNISLNFMTN 3672
            +C       Y   + + C+  PCK+NG C+  + ++Y+C+C   F GKNC+  I AC  QPC+NGG+C+ +D    Y C C  G+ G  C I    C  N C+NGA C  +N  + C C   F GK C+    YC +                       N C   G C       + C C  G+EG  CE + DDCK N CK+   C+D  N+++C+C  G+ GK CE           +     +  S+  Y +   E           +   S+C  + CLN G C     +NG Q       C C  G+ G +CQ+L         +I+  P  R  L     + +N   N
Sbjct:  432 LCQPGYTGKYCDKEINECVPNPCKNNGVCT-DSINDYKCNCSTSFKGKNCELNI-ACQNQPCKNGGVCQEVDT--GYTCKCKTGYTGTNCEIYKTGCSPNPCLNGAYCYTINQDFYCHCNKEFWGKRCEHSRPYCGNESCESAYDACSMPQMLNSSGIKHNYCGKYGTCLSHEKEKFICRCMPGFEGDSCEIDIDDCKTNPCKNGGICIDSINNFECICDNGFFGKTCEERTDYCIKSPCMNYGVCLRHSDELYNDFICECKNNWGGRLCHLPKHSICDSNPCLNGGSCSRLSTLNGFQ-------CHCNQGWIGTVCQFL---------HIDFQPCRRKNLCKNEGVCINVGYN 766          

HSP 2 Score: 111.694 bits (278), Expect = 2.602e-24
Identity = 70/201 (34.83%), Postives = 106/201 (52.74%), Query Frame = 1
Query: 2767 DSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDN--YGNYECVCPVGFRGPKCTINVDD-CVGNLCVNGATCNDLN--NSYSCKCQSGFRGKYCD---IKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEI 3345
            D C   PCK+ G C + + +N++C C   F GK C++R + C   PC N G+C    +  Y ++ C C   + G  C +     C  N C+NG +C+ L+  N + C C  G+ G  C    I F  C   N C+N G C  +  ND+ C C  G+EGK C+ NT++C  N C +  +C+D  N Y+C+C  G+ G  C++
Sbjct:  622 DDCKTNPCKNGGIC-IDSINNFECICDNGFFGKTCEERTDYCIKSPCMNYGVCLRHSDELYNDFICECKNNWGGRLCHLPKHSICDSNPCLNGGSCSRLSTLNGFQCHCNQGWIGTVCQFLHIDFQPCRRKNLCKNEGVCINVGYNDFTCHCKPGFEGKHCDINTNECNPNPCFNGGKCIDKLNSYRCICAKGFYGSNCQL 821          

HSP 3 Score: 111.694 bits (278), Expect = 3.006e-24
Identity = 101/319 (31.66%), Postives = 135/319 (42.32%), Query Frame = 1
Query: 2764 CDSCMKRP-CKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACF-GQPCRNGGICKNI-DNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQC----------------------------------------QLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEI-------PAWKPTVLKTLISRRDIN-QSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQY 3567
            CD CMK P CKH G C       + C C   + G+ CD+ +N C    PC+NGGIC+N+      Y+C+CP GF G  C I  D C  N C  G TC      + C+C   + GK CD     C D N CQN GQC                                        Q     DYKC+C  G+ GK C++  ++C  N CK+   C D  NDYKC C   + GK CE+       P     V + + +      ++  T  N E   I ++ C+ + CLN   C       NQ  YC C   + G  C++
Sbjct:  254 CDECMKYPGCKH-GTC---VDSPWTCSCENNWGGQFCDQSLNYCAENNPCKNGGICENLLSEVQPYKCICPSGFEGRDCEI--DPCFPNPCKKG-TCMKSKGEFYCQCIERWTGKLCDQDTSIC-DQNSCQNGGQCGKDENGNRRCICQQGYKGSECHLAAPCENSQCKHALDCQIQSTALGDYKCLCQPGYTGKYCDKEINECVPNPCKNNGVCTDSINDYKCNCSTSFKGKNCELNIACQNQPCKNGGVCQEVDTGYTCKCKTGYTGTNCE---IYKTGCSPNPCLNGAYCY----TINQDFYCHCNKEFWGKRCEH 557          

HSP 4 Score: 110.538 bits (275), Expect = 5.725e-24
Identity = 86/280 (30.71%), Postives = 132/280 (47.14%), Query Frame = 1
Query: 2788 CKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATC----NDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPN-NDYKCVCSKGWEGKQCERNTDDC----KYNSCKHESQCVDL-HNDYKCMCRPGYIGKFCEI--------PAWKP-TVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYL 3570
            C   G C       + C C   F G +C+  I+ C   PC+NGGIC  ID+  N+EC+C  GF G  C    D C+ + C+N   C    ++L N + C+C++ + G+ C +      D NPC N G C  L   N ++C C++GW G  C+    D     + N CK+E  C+++ +ND+ C C+PG+ GK C+I        P +     +  L S R I        N +   ++   CA   C    IC++    +     C+C  G  G LC ++
Sbjct:  590 CGKYGTCLSHEKEKFICRCMPGFEGDSCEIDIDDCKTNPCKNGGIC--IDSINNFECICDNGFFGKTCEERTDYCIKSPCMNYGVCLRHSDELYNDFICECKNNWGGRLCHLPKHSICDSNPCLNGGSCSRLSTLNGFQCHCNQGWIGTVCQFLHIDFQPCRRKNLCKNEGVCINVGYNDFTCHCKPGFEGKHCDINTNECNPNPCFNGGKCIDKLNSYRCICAKGFYGSNCQ---LNYDECASFPCAYGAICVD----RIDDFECRCPEGRDGKLCDHV 860          

HSP 5 Score: 104.76 bits (260), Expect = 3.800e-22
Identity = 71/201 (35.32%), Postives = 107/201 (53.23%), Query Frame = 1
Query: 2767 DSCMKRPCKHNGKC---SLITHHNYQCDCPYPFHGKNCD-KRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCT---INVDDCV-GNLCVNGATCNDLN-NSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCE 3342
            D C+K PC + G C   S   ++++ C+C   + G+ C   + + C   PC NGG C  +     ++C C  G+ G  C    I+   C   NLC N   C ++  N ++C C+ GF GK+CDI    CN  NPC N G+C +   N Y+C+C+KG+ G  C+ N D+C    C + + CVD  +D++C C  G  GK C+
Sbjct:  660 DYCIKSPCMNYGVCLRHSDELYNDFICECKNNWGGRLCHLPKHSICDSNPCLNGGSCSRLSTLNGFQCHCNQGWIGTVCQFLHIDFQPCRRKNLCKNEGVCINVGYNDFTCHCKPGFEGKHCDINTNECNP-NPCFNGGKC-IDKLNSYRCICAKGFYGSNCQLNYDECASFPCAYGAICVDRIDDFECRCPEGRDGKLCD 858          

HSP 6 Score: 88.5817 bits (218), Expect = 3.563e-17
Identity = 92/358 (25.70%), Postives = 148/358 (41.34%), Query Frame = 1
Query: 2626 FLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKCD---SCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKC-----------------------TINVDDCVGNLCVNGATC-NDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCV----DLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMT----YENLENEVISESVCAY-----------HECLNNGICINGDQNKNQKSYCKCKPGYSGHLC 3561
            +  ++ D  I+ C  P   K   +      D+ C+  T  ++    C+   +C  +PCK+ G C  +    Y C C   + G NC+     C   PC NG  C  I+   ++ C C   F G +C                        +N      N C    TC +     + C+C  GF G  C+I    C   NPC+N G C +   N+++C+C  G+ GK CE  TD C  + C +   C+    +L+ND+ C C+  + G+ C +P  K ++  +       + S ++    ++   N+    +VC +           + C N G+CIN   N      C CKPG+ G  C
Sbjct:  437 YTGKYCDKEINECV-PNPCKNNGVCTDSINDYKCNCST--SFKGKNCELNIACQNQPCKNGGVCQEVDT-GYTCKCKTGYTGTNCEIYKTGCSPNPCLNGAYCYTINQ--DFYCHCNKEFWGKRCEHSRPYCGNESCESAYDACSMPQMLNSSGIKHNYCGKYGTCLSHEKEKFICRCMPGFEGDSCEIDIDDCK-TNPCKNGGIC-IDSINNFECICDNGFFGKTCEERTDYCIKSPCMNYGVCLRHSDELYNDFICECKNNWGGRLCHLP--KHSICDSNPCLNGGSCSRLSTLNGFQCHCNQGWIGTVCQFLHIDFQPCRRKNLCKNEGVCINVGYNDFT---CHCKPGFEGKHC 781          
BLAST of slit1 vs. Planmine SMEST
Match: SMESG000022173.1 (SMESG000022173.1)

HSP 1 Score: 120.168 bits (300), Expect = 6.082e-27
Identity = 87/296 (29.39%), Postives = 127/296 (42.91%), Query Frame = 1
Query: 2800 GKCSL-ITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNS-CKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICI--------NGDQNKNQKSY-----CKCKPGYSGHLCQYLNSIYFQKNSYIEISPP---SRGFL 3633
            GKC + +  + Y CDCP  F+G  C     +C   PC     C   D    ++C CP GF G  C I+ D+C    C+NG  C D  + + C C  GF G  C +    C  ++ C N  +C +   N   C C+ GW G+ C +N ++C  N  C +   C+D    Y+C C+  + G+FC +    P +    + R+                     C  + C NNGICI        N   N+  K       CKC PG++G LC+    +     S +  S P   + GFL
Sbjct:  330 GKCKINVNTNKYICDCPESFYGDRCQFNQTSCDDNPCVGNSECI-TDKTSGFKCKCPPGFTGRNCEIDEDECSSKPCLNGGMCIDRPSGFLCVCPVGFSGDRCQVNKNDCQKLS-CMNGAKC-MDRANRIDCECTTGWTGRMCNQNINECAINQPCLNNGICIDTPGSYRCQCQATWSGQFCNLKIVVPNI--NYLERK---------------------CLINPCRNNGICILNSLPTSVNIPNNEYFKPIGGPYTCKCNPGFTGDLCE---KVIIANGSIVMYSEPRITTEGFL 596          

HSP 2 Score: 90.8929 bits (224), Expect = 6.570e-18
Identity = 82/293 (27.99%), Postives = 121/293 (41.30%), Query Frame = 1
Query: 2740 EDAYITAKCDSCMKRPCKHNGKCSLITHHN----YQCDCPYPFHGKNCDKRINACFGQP--CRNGGICKNIDNYGN---YECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTL--------ISRRDINQSEMTYENLENEVISESVCAYHE-CLNNGICINGDQNKNQKSY-CKCKPGYSGHLC 3561
            +  +    CD C+  P   +G C             C+C   + G  C+  +N C   P  C NGG C N  N      Y C+C  G    KC INV+                 N Y C C   F G  C      C+D NPC  + +C     + +KC C  G+ G+ CE + D+C    C +   C+D  + + C+C  G+ G  C++       L  +         +R D   +      + N+ I+E  CA ++ CLNNGICI+        SY C+C+  +SG  C
Sbjct:  244 QTGWTGDSCDQCIPYPGCVHGSCRFDETTKELLPLTCNCKKKWGGMFCNIDLNYCSNNPNVCENGGECLNFGNIKTNQQYRCICKPG----KCKINVN----------------TNKYICDCPESFYGDRCQFNQTSCDD-NPCVGNSECITDKTSGFKCKCPPGFTGRNCEIDEDECSSKPCLNGGMCIDRPSGFLCVCPVGFSGDRCQVNKNDCQKLSCMNGAKCMDRANRIDCECTTGWTGRMCNQNINE--CAINQPCLNNGICID-----TPGSYRCQCQATWSGQFC 508          

HSP 3 Score: 58.9214 bits (141), Expect = 3.642e-8
Identity = 76/308 (24.68%), Postives = 110/308 (35.71%), Query Frame = 1
Query: 2830 YQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCD---------------------------------------IKFLYC-NDINPCQNSGQC----QLLPNNDYKCVCSKG------------------WEGKQCERNTDDCKYNSCKHESQCV-DLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQ 3564
            ++  C   +HG+ CDK       +P  N     N  + G+   +C  G+ G  C   V  C+   C+NGA C   N    C+CQ+G+ G  CD                                       I   YC N+ N C+N G+C     +  N  Y+C+C  G                  + G +C+ N   C  N C   S+C+ D  + +KC C PG+ G+ CE                                I E  C+   CLN G+CI    ++     C C  G+SG  CQ
Sbjct:  175 FRYTCSQNYHGEECDK-----LCKPSANERGFYNCSSNGDR--ICHTGYSGSYCDKVV--CLKK-CINGA-CVGPN---QCRCQTGWTGDSCDQCIPYPGCVHGSCRFDETTKELLPLTCNCKKKWGGMFCNIDLNYCSNNPNVCENGGECLNFGNIKTNQQYRCICKPGKCKINVNTNKYICDCPESFYGDRCQFNQTSCDDNPCVGNSECITDKTSGFKCKCPPGFTGRNCE--------------------------------IDEDECSSKPCLNGGMCI----DRPSGFLCVCPVGFSGDRCQ 432          
The following BLAST results are available for this feature:
BLAST of slit1 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
SLIT32.377e-4933.75slit guidance ligand 3 [Source:HGNC Symbol;Acc:HGN... [more]
SLIT32.959e-4933.60slit guidance ligand 3 [Source:HGNC Symbol;Acc:HGN... [more]
SLIT21.038e-732.84slit guidance ligand 2 [Source:HGNC Symbol;Acc:HGN... [more]
SLIT21.029e-732.77slit guidance ligand 2 [Source:HGNC Symbol;Acc:HGN... [more]
SLIT29.559e-832.47slit guidance ligand 2 [Source:HGNC Symbol;Acc:HGN... [more]
back to top
BLAST of slit1 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
slt-10.000e+029.47Slit homolog 1 protein [Source:UniProtKB/Swiss-Pr... [more]
clec-788.237e-3936.23C-type LECtin [Source:UniProtKB/TrEMBL;Acc:Q20531... [more]
Y46G5A.297.483e-2542.95pep chromosome:WBcel235:II:12856200:12862058:1 gen... [more]
glp-11.098e-2227.76pep chromosome:WBcel235:III:9092241:9099698:1 gene... [more]
crb-14.185e-2228.92Drosophila CRumBs homolog [Source:UniProtKB/TrEMB... [more]
back to top
BLAST of slit1 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
sli2.002e-4532.27gene:FBgn0264089 transcript:FBtr0330730[more]
sli2.315e-4531.99gene:FBgn0264089 transcript:FBtr0330729[more]
sli2.315e-4531.99gene:FBgn0264089 transcript:FBtr0330732[more]
sli5.066e-4331.66gene:FBgn0264089 transcript:FBtr0330734[more]
sli1.338e-4331.55gene:FBgn0264089 transcript:FBtr0330733[more]
back to top
BLAST of slit1 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
slit21.414e-733.09slit homolog 2 (Drosophila) [Source:ZFIN;Acc:ZDB-G... [more]
slit21.798e-733.09slit homolog 2 (Drosophila) [Source:ZFIN;Acc:ZDB-G... [more]
slit21.641e-732.88slit homolog 2 (Drosophila) [Source:ZFIN;Acc:ZDB-G... [more]
slit21.452e-732.88slit homolog 2 (Drosophila) [Source:ZFIN;Acc:ZDB-G... [more]
slit37.392e-7931.42slit homolog 3 (Drosophila) [Source:ZFIN;Acc:ZDB-G... [more]
back to top
BLAST of slit1 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
zxdc3.111e-5131.58ZXD family zinc finger C [Source:Xenbase;Acc:XB-GE... [more]
atad3a1.103e-733.55ATPase family, AAA domain containing 3A [Source:Xe... [more]
atad3a2.284e-733.64ATPase family, AAA domain containing 3A [Source:Xe... [more]
atad3a2.406e-733.67ATPase family, AAA domain containing 3A [Source:Xe... [more]
atad3a1.057e-733.50ATPase family, AAA domain containing 3A [Source:Xe... [more]
back to top
BLAST of slit1 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Slit39.019e-4733.89slit guidance ligand 3 [Source:MGI Symbol;Acc:MGI:... [more]
Slit21.548e-732.77slit guidance ligand 2 [Source:MGI Symbol;Acc:MGI:... [more]
Slit21.536e-732.60slit guidance ligand 2 [Source:MGI Symbol;Acc:MGI:... [more]
Slit21.534e-732.70slit guidance ligand 2 [Source:MGI Symbol;Acc:MGI:... [more]
Slit21.462e-732.40slit guidance ligand 2 [Source:MGI Symbol;Acc:MGI:... [more]
back to top
BLAST of slit1 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q9WVB4|SLIT3_MOUSE6.309e-4633.89Slit homolog 3 protein OS=Mus musculus OX=10090 GN... [more]
sp|O88280|SLIT3_RAT1.060e-4533.60Slit homolog 3 protein OS=Rattus norvegicus OX=101... [more]
sp|P24014|SLIT_DROME1.342e-4231.55Protein slit OS=Drosophila melanogaster OX=7227 GN... [more]
sp|O75094|SLIT3_HUMAN1.141e-4833.75Slit homolog 3 protein OS=Homo sapiens OX=9606 GN=... [more]
sp|O94813|SLIT2_HUMAN4.987e-732.60Slit homolog 2 protein OS=Homo sapiens OX=9606 GN=... [more]
back to top
BLAST of slit1 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A1XCJ50.000e+099.41Slit1 OS=Schmidtea mediterranea OX=79327 PE=2 SV=2[more]
E1CHK50.000e+081.45Slit OS=Dugesia japonica OX=6161 GN=Djslit PE=2 SV... [more]
A0A4E0S2Z53.039e-3133.93Axon guidance protein OS=Fasciola hepatica OX=6192... [more]
A0A267F2C19.865e-733.76Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A267F5J31.292e-633.70Uncharacterized protein OS=Macrostomum lignano OX=... [more]
back to top
BLAST of slit1 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
slit37.516e-7731.17slit guidance ligand 3 [Source:NCBI gene;Acc:10303... [more]
slit31.011e-7630.88slit guidance ligand 3 [Source:NCBI gene;Acc:10303... [more]
slit37.919e-7731.17slit guidance ligand 3 [Source:NCBI gene;Acc:10303... [more]
slit39.844e-7730.33slit guidance ligand 3 [Source:NCBI gene;Acc:10303... [more]
slit1a1.205e-3528.64slit homolog 1a (Drosophila) [Source:ZFIN;Acc:ZDB-... [more]
back to top
BLAST of slit1 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSPMAT00000003392.11.629e-5925.24pep scaffold:Pmarinus_7.0:GL476560:299294:319002:1... [more]
ENSPMAT00000002643.14.952e-3331.58pep scaffold:Pmarinus_7.0:GL478752:33855:40482:1 g... [more]
ENSPMAT00000002014.14.894e-3233.33pep scaffold:Pmarinus_7.0:GL482490:2146:12219:-1 g... [more]
ENSPMAT00000005361.15.069e-3036.73pep scaffold:Pmarinus_7.0:GL479377:6554:13965:1 ge... [more]
JAG12.270e-2833.03jagged canonical Notch ligand 1 [Source:HGNC Symbo... [more]
back to top
BLAST of slit1 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of slit1 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO488512.149e-4234.84Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO465131.000e-3936.11Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO391653.521e-3734.59Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO426445.396e-3743.01Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO361951.304e-3636.06Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
back to top
BLAST of slit1 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
slit21.810e-733.64slit guidance ligand 2 [Source:NCBI gene;Acc:10117... [more]
slit21.078e-633.33slit guidance ligand 2 [Source:NCBI gene;Acc:10117... [more]
slit21.096e-633.29slit guidance ligand 2 [Source:NCBI gene;Acc:10117... [more]
slit21.317e-632.91slit guidance ligand 2 [Source:NCBI gene;Acc:10117... [more]
slit1a1.806e-4730.39slit guidance ligand 1 [Source:NCBI gene;Acc:10116... [more]
back to top
BLAST of slit1 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000068721.10.000e+099.80SMESG000068721.1[more]
SMESG000063217.12.602e-3134.15SMESG000063217.1[more]
SMESG000027402.17.828e-3129.58SMESG000027402.1[more]
SMESG000027402.18.619e-3129.58SMESG000027402.1[more]
SMESG000022173.16.082e-2729.39SMESG000022173.1[more]
back to top
Cross References
External references for this transcript
DatabaseAccession
SmedGD GBrowseSMED30023930
GenBankABC61956.2
Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30023930 ID=SMED30023930|Name=slit1|organism=Schmidtea mediterranea sexual|type=transcript|length=4812bp
ATTAAGTTATCAAATATAAGTATTTTATAAACACGAGAAAATATGATTTT
TATTTTCAAGGATATTACATTTAAATTTTTGTTGGTTTTATTGTCATCGC
TCTATTGTTTTTCGTCGACAATTTGGATCAATGATCCGTCTGACGAATCA
GAAATCTGTCCAAATGGGTGTCAATGTGAAAACTATTCAGTCGAATGCAA
ACATCGTTTGTTTCCAAACGTTCCGGTTATTTTACAAGAAAAAACTACAA
GACTAAATCTTCTTGGAAATCGAATCAATGTCATCAGAAAGTCAGATTTT
TTAAAATTAAATAACCTAAAAGTTTTGCAATTGTCAAACAACAGAATTAC
TTCAATTGAGCCAGGAGCGTTTGACAATCTAGTCAATCTTATCAAATTAC
GATTAAATCGTAATCTGATTCAATATCTTCCAGATGATCTGTTTTCCAAA
TTGACCAAATTACAAAAATTAGATCTCCGTGACAATCAGCTGCAATGCAT
CAATGACAACATTTTCAACAAGCTCGGGATGCTCAAATATTTACTTTTAG
AAGGTAACAAAATATTTTGGATATCAGAAGCCATATCAAAACTGAAACAT
TTAAAAGAATTACGCATACATAAAAACAAATTAAATTGTAACTGTCATCT
AGCTTGGCTTAATTGGTTTTTAAAACAAAAATCAAATATCATTAAGCCAG
GAGCTCATTCCGTTTTATGTTCGACGCCGTTCAGACTCTCTCATATTCCA
ATTTCTAGTCTCAGTTCTTATGAGTTTCAATGTGCAAGTGAAGACAAGGC
AAACCTAGAAAACATGTGCCCGTCAGATAAAAAGGTCTGCTCAAAAAAAT
GTAATTGTTCTCATGATGGAATTGTGAAATGCGTTGGGAAAAGGTTGTCC
AATATTCCTCAGGATCTACCGACAAATGTTGTGGAATTAGACTTGAAGGA
CAACAACATTACGATCCTCCAACAAGGATACCTGTCTAAGTATAAGTTTT
TGAAAAAGATAATTCTCACACGGAACGGAATGGTTGACATTGAACCTGGC
GCATTTTTGGGATTAACCCAACTGACATCTATTTATTTGAATGAAAACAA
TTTGAAAACAATCAGAAAAAACGCTTTTGGTGGATTGGTATCTCTAATGT
TTTTATATTTACAATACAACGACATCGAGTGTCTACCAAAGGATACCCTA
GAAAGCTCCAAGCGACTTCATTTATTACACCTTTCTGAAAACAAATTAAC
TTCTCTCAAGAAAGAAACATTCAAACCATTTTTAAATCTCAGATATTTGT
ATCTGGTTCATAACCCGTTGAATTGCGATTGCAGAATGTCCTGGATTGTA
GATTACATAAAAGATAAAGAGACTAATACTCCGGTGGCCGCATGCCTGCA
GCCAAGGAATTTGCAAGGTACCCCGTTGAGGAATCTTCAGATTTACAATT
TTCAATGCAACAAGGAATTTGAGTCAAAATATGAAACACATGATGAAGAA
TGTGTACCCAAAGTCAGTTGTCCCTTAAAATGTACTTGTTCATCTGACGT
AGTCAACTGTTCAAATTCTGGATTGATGTCGCTTCCAGAAAGTATTTTCC
CATCTACAAAAACGCTAATCATGTCGCATAATAATTTGAAAAAGTTACCT
CCCATTTATTCCATTCACGAAGCTCCCAATCTAATGAAATTAGATTTATC
ATTCAACCAAATAGAAACTCTTGAACCGGACGTTTTCACAAGAAGTTCAA
AATTACGAGAAATAAGTTTAAATTCTAACAGATTGAAATGCATTAACAAT
GAAACATTCAAGAATCTGAAGGAATTAGAAATTTTACAATTAAGCGAGAA
TGCTATCAGCTGTATCGTCACGGATTCATTCAAAAATAATCTGAATTTGA
AATATTTAATGCTGAATAAGAATCAGTTCCATTGCGATTGTAAGTTGAAG
TGGATGTCGGAATGGTCAAGAAATCACTATACAGTTCTCGGCAATCATCG
GCTTCCGACTTGCCAGTCACCAATCATATTGAAAGATACTCCCATTACAC
ATTTGGATGACAAGTATTTTCTATGTGACGGTAATGCGTCTGTTTACCAT
TCGGTTGATTCGTGTCATGAAACAAATTCCGTGAAGACCTGCTGCTCAAT
TGGAAATGAAGAGTGCACTAAATCCAAAAAATGTCCTTCGAAATGCAAAT
GCGAATCAACCAAAGTGGATTGTAGTGATTTACAGTTAAAAGAAATTCCA
AATGAAATTCCAAAAGATACTACAGAATTATATCTCGACCGAAACCATCT
GCAAAGTCTAAATGAAACATCTTTCAAGAATTTATTAAATTTAAAAACTT
TAGTTCTCAGCTATAATGGTATTACTGAATTGAACAAGAATGTTTTGACT
CCATTGAAAAGTCTGGAAACCTTAGTGTTGAGCTTCAACAAATTACAATG
CATTCATCAGGATGCCTTCAAAAATCTACACAATTTAAAAGTTCTAATAC
TCCAAAGTAATGATGTTTCTACGATTCCTTACCAAGCTTTTAACGACCTG
AAAAATCTCAATAACATAGCTCTGGGACAAAATCCATTTCATTGTGACTG
CAATATAAAGTGGTTAAACCAGTTCTTTTTAGATAGATTCCTGGATAATG
GAATCTCATTGTGTGCTAGTCCTGAAAAAATGAAATTAAAGTCTATTTAC
CATTCCAAACCAACTGATTTCATTTGTTCTGAAGAAACCGAAGATGCTTA
TATTACTGCTAAGTGTGACAGTTGCATGAAGAGACCATGCAAACATAACG
GAAAATGTTCCTTGATAACCCATCATAATTATCAATGTGATTGTCCTTAT
CCATTTCATGGGAAAAACTGTGACAAAAGAATAAATGCCTGTTTTGGACA
GCCATGCAGAAATGGTGGGATTTGCAAAAATATTGATAATTACGGAAACT
ATGAATGTGTTTGTCCAGTTGGTTTTCGTGGTCCAAAATGTACAATAAAT
GTTGACGATTGTGTGGGTAATTTATGTGTAAATGGAGCGACCTGTAATGA
TCTTAACAATAGCTACAGTTGTAAATGTCAAAGTGGTTTCCGAGGTAAAT
ATTGCGACATCAAATTTTTATACTGCAATGACATTAATCCATGCCAAAAT
TCTGGTCAATGTCAATTATTGCCAAATAATGATTACAAATGCGTTTGTTC
AAAAGGTTGGGAAGGCAAGCAATGTGAACGGAATACTGATGATTGCAAGT
ACAACAGCTGTAAACACGAATCCCAATGCGTAGATTTGCACAATGATTAC
AAGTGCATGTGCAGACCCGGTTACATTGGTAAATTCTGTGAAATCCCGGC
ATGGAAGCCCACGGTGTTGAAGACTTTAATATCTCGAAGGGATATTAATC
AGAGCGAAATGACTTATGAAAATTTAGAGAACGAGGTGATATCAGAATCA
GTTTGCGCTTACCACGAATGCCTAAATAACGGAATTTGTATAAATGGAGA
TCAAAATAAAAATCAGAAATCGTATTGCAAGTGTAAACCCGGATATTCTG
GACATCTCTGTCAATATTTGAATAGCATATATTTTCAAAAGAATTCTTAC
ATTGAAATCTCTCCTCCGTCGAGAGGATTTCTCATGCCAAGAGGAAATAT
TTCCCTAAATTTTATGACGAATTCTTCATCTGGTCTTATATTATATCAAG
GCTCTGAACAAACTTATCTCGTTGTAAATCTATATCAAGGTCATTTAAGA
ATTAGTTATAATCTTGGTGATAAGGTTGTTGGTCCCGAAGGTTATTCGAT
GGGAATGGTCAATGATAGTAGATTGCATGCGTTACATATTGAAATTGTCG
GTGAAAACTTAACGATGTACTTGAATGGTCGGGTTCACTGCACACTTAAA
TCAAAGTATTTAAACTCCAATCGGTACATGGCACTTGATACAGGGATTTT
TATTGGGGGAGCACCGCATAAAATTCTCCAGTCGGCCATAATGAAATTCC
ACATAGAAACCGATTCAGAATTTATTGGTTGCATGGAATCTCTGTTAATT
AATCGGAAAAAAATCGATTTCTCTAAATATCTACCAAAATCCATAAATGT
GCACCCAGGGTGCGGAAAACTGATATCAACTGTTGTTGCGTCAGATTTGA
AAATCCCGTCAATTATGCAACCCATTAAGCAGATTCTGACACAAACTACA
AAAAAGAAACCCAACAATGTTTGCAAGCGACCAGGAATTTGTAAAAATCA
AGGAGTCTGTGAAGTTCTTCCTGGCAGTAAGAAAAAGTTTCGATGCATAT
GTAAAAACGGTTTTGTTGGAAGAAAATGCAAAAAAAGAAAGTCAATGTGC
CATGGGACATTTACTGAAAGCCATATTGTTGACCCTGATTCTCAAGGGAC
CTGCAAGTCAATTGAAAGATTTCAATACCGAATTTGTACTGGAACTTGCA
GCACCAGTCGAATGCAATCGGATGTCATCAAGAATTTTTATCACAAGCCC
ATAGATCCCAGCTCATCTCGTTGCTGTCAACCAACCAAGTACTCCACTCA
TGAAATTCAGTTCAAGTGCGAAAACGGACGCGTATATCGAAGGGCTGCAA
ACCTTCCGAAAAATTGCAAATGTGTAGCTAACTGCCTAAATGATTGATCG
ATGGAGAATTTCCATTTTTTTTCTAAATTTTTCATGCCAAAATAAAAACC
AACTAATTTTTTATTTTTCTTAATCTATACTATGATTTATGTTTCGTCCA
AATTTCACAAATGATCCAATTAACAAATACTTAAAAGTCATAAGTCTCAT
CATATCCGTTAT
back to top

protein sequence of SMED30023930-orf-1

>SMED30023930-orf-1 ID=SMED30023930-orf-1|Name=SMED30023930-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=1535bp
MIFIFKDITFKFLLVLLSSLYCFSSTIWINDPSDESEICPNGCQCENYSV
ECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNN
RITSIEPGAFDNLVNLIKLRLNRNLIQYLPDDLFSKLTKLQKLDLRDNQL
QCINDNIFNKLGMLKYLLLEGNKIFWISEAISKLKHLKELRIHKNKLNCN
CHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCASE
DKANLENMCPSDKKVCSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELD
LKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLN
ENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSEN
KLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAA
CLQPRNLQGTPLRNLQIYNFQCNKEFESKYETHDEECVPKVSCPLKCTCS
SDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLPPIYSIHEAPNLMKL
DLSFNQIETLEPDVFTRSSKLREISLNSNRLKCINNETFKNLKELEILQL
SENAISCIVTDSFKNNLNLKYLMLNKNQFHCDCKLKWMSEWSRNHYTVLG
NHRLPTCQSPIILKDTPITHLDDKYFLCDGNASVYHSVDSCHETNSVKTC
CSIGNEECTKSKKCPSKCKCESTKVDCSDLQLKEIPNEIPKDTTELYLDR
NHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVLSFNK
LQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFH
CDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETE
DAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINAC
FGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGAT
CNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKC
VCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCE
IPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICI
NGDQNKNQKSYCKCKPGYSGHLCQYLNSIYFQKNSYIEISPPSRGFLMPR
GNISLNFMTNSSSGLILYQGSEQTYLVVNLYQGHLRISYNLGDKVVGPEG
YSMGMVNDSRLHALHIEIVGENLTMYLNGRVHCTLKSKYLNSNRYMALDT
GIFIGGAPHKILQSAIMKFHIETDSEFIGCMESLLINRKKIDFSKYLPKS
INVHPGCGKLISTVVASDLKIPSIMQPIKQILTQTTKKKPNNVCKRPGIC
KNQGVCEVLPGSKKKFRCICKNGFVGRKCKKRKSMCHGTFTESHIVDPDS
QGTCKSIERFQYRICTGTCSTSRMQSDVIKNFYHKPIDPSSSRCCQPTKY
STHEIQFKCENGRVYRRAANLPKNCKCVANCLND*
back to top
Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000028Category 2 cell
PLANA:0000030midline
PLANA:0000032dorsal midline
PLANA:0000038ventral midline
PLANA:0000081pharynx pouch
PLANA:0000101muscle cell
PLANA:0002056medial muscle cell
Vocabulary: INTERPRO
TermDefinition
IPR000372LRRNT
IPR001791Laminin_G
IPR000483Cys-rich_flank_reg_C
IPR003591Leu-rich_rpt_typical-subtyp
IPR000742EGF-like_dom
IPR001881EGF-like_Ca-bd_dom
IPR006207Cys_knot_C
IPR032675LRR_dom_sf
IPR013320ConA-like_dom_sf
IPR001611Leu-rich_rpt
IPR000152EGF-type_Asp/Asn_hydroxyl_site
IPR013032EGF-like_CS
IPR018097EGF_Ca-bd_CS
Vocabulary: molecular function
TermDefinition
GO:0005515protein binding
GO:0005509calcium ion binding
Vocabulary: cellular component
TermDefinition
GO:0016020membrane
GO:0016021integral component of membrane
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000372Leucine-rich repeat N-terminal domainSMARTSM00013lrrnt1coord: 713..745
e-value: 1.0E-8
score: 45.0
coord: 38..70
e-value: 2.4
score: 14.7
coord: 265..298
e-value: 0.062
score: 22.4
coord: 492..524
e-value: 7.4E-4
score: 28.8
IPR000372Leucine-rich repeat N-terminal domainPFAMPF01462LRRNTcoord: 714..740
e-value: 1.0E-6
score: 28.3
IPR000483Cysteine-rich flanking region, C-terminalSMARTSM00082lrrct1coord: 424..473
e-value: 5.6E-6
score: 35.9
coord: 847..896
e-value: 7.5E-10
score: 48.7
coord: 627..679
e-value: 0.0012
score: 28.1
coord: 195..248
e-value: 0.0073
score: 25.5
IPR001881EGF-like calcium-binding domainSMARTSM00179egfca_6coord: 1064..1100
e-value: 1.2E-6
score: 38.0
coord: 946..984
e-value: 5.0E-4
score: 28.5
coord: 986..1022
e-value: 5.1E-8
score: 42.7
coord: 1030..1062
e-value: 0.0062
score: 19.6
coord: 1392..1430
e-value: 0.97
score: 1.9
IPR003591Leucine-rich repeat, typical subtypeSMARTSM00369LRR_typ_2coord: 569..591
e-value: 29.0
score: 9.5
coord: 89..112
e-value: 4.2E-5
score: 33.0
coord: 137..160
e-value: 0.0067
score: 25.6
coord: 390..412
e-value: 22.0
score: 10.4
coord: 113..136
e-value: 0.0044
score: 26.2
coord: 744..763
e-value: 110.0
score: 4.8
coord: 365..388
e-value: 0.91
score: 18.6
coord: 161..184
e-value: 30.0
score: 9.3
coord: 592..615
e-value: 3.2
score: 16.7
coord: 545..567
e-value: 2.3
score: 17.2
coord: 764..787
e-value: 4.9
score: 15.8
coord: 812..835
e-value: 0.061
score: 22.5
coord: 788..811
e-value: 0.0025
score: 27.1
coord: 341..364
e-value: 0.0066
score: 25.7
IPR001791Laminin G domainSMARTSM00282LamG_3coord: 1199..1339
e-value: 7.8E-21
score: 85.2
IPR001791Laminin G domainPFAMPF00054Laminin_G_1coord: 1207..1342
e-value: 1.5E-15
score: 57.6
IPR001791Laminin G domainPROSITEPS50025LAM_G_DOMAINcoord: 1176..1357
score: 30.959
IPR006207Cystine knot, C-terminalSMARTSM00041CT_3coord: 1437..1532
e-value: 0.0024
score: 17.7
IPR006207Cystine knot, C-terminalPROSITEPS01185CTCK_1coord: 1494..1531
IPR006207Cystine knot, C-terminalPROSITEPS01225CTCK_2coord: 1429..1532
score: 12.026
NoneNo IPR availableSMARTSM00365LRR_sd22_2coord: 413..430
e-value: 280.0
score: 3.7
coord: 137..159
e-value: 40.0
score: 10.7
coord: 544..565
e-value: 400.0
score: 2.5
coord: 592..613
e-value: 310.0
score: 3.4
coord: 764..782
e-value: 500.0
score: 1.7
coord: 568..589
e-value: 570.0
score: 1.2
coord: 341..362
e-value: 500.0
score: 1.7
coord: 89..110
e-value: 14.0
score: 14.4
coord: 293..316
e-value: 120.0
score: 6.9
coord: 788..814
e-value: 130.0
score: 6.4
NoneNo IPR availableGENE3DG3DSA:2.10.25.10coord: 1067..1106
e-value: 3.0E-10
score: 41.6
NoneNo IPR availableGENE3DG3DSA:2.10.25.10coord: 988..1024
e-value: 3.7E-10
score: 41.5
NoneNo IPR availableGENE3DG3DSA:2.10.25.10coord: 1027..1066
e-value: 2.1E-8
score: 36.1
NoneNo IPR availableGENE3DG3DSA:2.10.25.10coord: 1381..1431
e-value: 4.1E-5
score: 25.3
NoneNo IPR availableGENE3DG3DSA:2.10.25.10coord: 1107..1177
e-value: 1.3E-7
score: 33.6
NoneNo IPR availableGENE3DG3DSA:2.60.120.200coord: 1178..1358
e-value: 8.5E-29
score: 102.5
NoneNo IPR availableGENE3DG3DSA:2.10.25.10coord: 904..949
e-value: 1.7E-7
score: 32.6
coord: 950..987
e-value: 6.2E-10
score: 40.5
NoneNo IPR availablePANTHERPTHR45836:SF4PROTEIN SLITcoord: 35..169
NoneNo IPR availablePANTHERPTHR45836:SF4PROTEIN SLITcoord: 569..815
coord: 148..576
coord: 731..1339
NoneNo IPR availablePANTHERPTHR45836FAMILY NOT NAMEDcoord: 569..815
coord: 148..576
coord: 731..1339
NoneNo IPR availablePANTHERPTHR45836FAMILY NOT NAMEDcoord: 35..169
NoneNo IPR availableCDDcd00110LamGcoord: 1178..1337
e-value: 1.21691E-24
score: 99.4148
NoneNo IPR availableCDDcd00054EGF_CAcoord: 986..1021
e-value: 1.0419E-7
score: 47.2462
NoneNo IPR availableCDDcd00054EGF_CAcoord: 1138..1174
e-value: 0.00661262
score: 33.7642
NoneNo IPR availableCDDcd00054EGF_CAcoord: 1394..1430
e-value: 6.14757E-5
score: 39.5422
NoneNo IPR availableCDDcd00054EGF_CAcoord: 1028..1062
e-value: 7.8846E-6
score: 42.2386
NoneNo IPR availableCDDcd00054EGF_CAcoord: 1064..1100
e-value: 8.66287E-7
score: 44.935
NoneNo IPR availableCDDcd00054EGF_CAcoord: 947..984
e-value: 6.4786E-6
score: 42.2386
NoneNo IPR availableSIGNALP_EUKSignalP-noTMSignalP-noTMcoord: 1..24
score: 0.723
NoneNo IPR availableSUPERFAMILYSSF57196EGF/Laminincoord: 1064..1105
NoneNo IPR availableSUPERFAMILYSSF52058L domain-likecoord: 492..862
NoneNo IPR availableSUPERFAMILYSSF57196EGF/Laminincoord: 905..946
NoneNo IPR availableSUPERFAMILYSSF52058L domain-likecoord: 362..438
NoneNo IPR availableSUPERFAMILYSSF57196EGF/Laminincoord: 946..990
NoneNo IPR availableSUPERFAMILYSSF52058L domain-likecoord: 62..666
NoneNo IPR availableSUPERFAMILYSSF57196EGF/Laminincoord: 1027..1070
NoneNo IPR availableSUPERFAMILYSSF57196EGF/Laminincoord: 1394..1438
NoneNo IPR availableSUPERFAMILYSSF57196EGF/Laminincoord: 983..1024
IPR000742EGF-like domainSMARTSM00181egf_5coord: 949..984
e-value: 1.7E-4
score: 31.0
coord: 1067..1100
e-value: 2.3E-4
score: 30.5
coord: 910..944
e-value: 0.019
score: 24.2
coord: 1393..1430
e-value: 0.0025
score: 27.0
coord: 1027..1062
e-value: 1.6E-4
score: 31.1
coord: 1137..1174
e-value: 0.0058
score: 25.8
coord: 989..1022
e-value: 1.8E-5
score: 34.2
IPR000742EGF-like domainPFAMPF00008EGFcoord: 1028..1059
e-value: 2.2E-6
score: 27.8
IPR000742EGF-like domainPROSITEPS50026EGF_3coord: 1024..1062
score: 18.53
IPR000742EGF-like domainPROSITEPS50026EGF_3coord: 946..984
score: 20.519
IPR000742EGF-like domainPROSITEPS50026EGF_3coord: 986..1022
score: 20.866
IPR000742EGF-like domainPROSITEPS50026EGF_3coord: 909..944
score: 13.749
IPR000742EGF-like domainPROSITEPS50026EGF_3coord: 1064..1100
score: 19.295
IPR000742EGF-like domainPROSITEPS50026EGF_3coord: 1390..1430
score: 14.749
IPR000742EGF-like domainPROSITEPS50026EGF_3coord: 1134..1174
score: 16.544
IPR001611Leucine-rich repeatPFAMPF13855LRR_8coord: 521..581
e-value: 3.9E-10
score: 39.4
coord: 69..126
e-value: 4.1E-12
score: 45.7
coord: 320..378
e-value: 4.7E-15
score: 55.1
coord: 744..801
e-value: 2.3E-12
score: 46.5
IPR001611Leucine-rich repeatPROSITEPS51450LRRcoord: 766..787
score: 7.065
IPR001611Leucine-rich repeatPROSITEPS51450LRRcoord: 546..567
score: 7.843
IPR001611Leucine-rich repeatPROSITEPS51450LRRcoord: 742..763
score: 5.425
IPR001611Leucine-rich repeatPROSITEPS51450LRRcoord: 186..207
score: 5.302
IPR001611Leucine-rich repeatPROSITEPS51450LRRcoord: 415..436
score: 4.886
IPR001611Leucine-rich repeatPROSITEPS51450LRRcoord: 67..88
score: 5.979
IPR001611Leucine-rich repeatPROSITEPS51450LRRcoord: 343..364
score: 5.471
IPR001611Leucine-rich repeatPROSITEPS51450LRRcoord: 594..615
score: 6.38
IPR001611Leucine-rich repeatPROSITEPS51450LRRcoord: 295..316
score: 5.225
IPR001611Leucine-rich repeatPROSITEPS51450LRRcoord: 618..639
score: 4.809
IPR001611Leucine-rich repeatPROSITEPS51450LRRcoord: 367..388
score: 6.38
IPR001611Leucine-rich repeatPROSITEPS51450LRRcoord: 521..542
score: 5.717
IPR001611Leucine-rich repeatPROSITEPS51450LRRcoord: 814..835
score: 6.21
IPR001611Leucine-rich repeatPROSITEPS51450LRRcoord: 319..340
score: 5.14
IPR001611Leucine-rich repeatPROSITEPS51450LRRcoord: 139..160
score: 7.519
IPR001611Leucine-rich repeatPROSITEPS51450LRRcoord: 391..412
score: 6.849
IPR001611Leucine-rich repeatPROSITEPS51450LRRcoord: 91..112
score: 8.721
IPR001611Leucine-rich repeatPROSITEPS51450LRRcoord: 570..591
score: 7.08
IPR001611Leucine-rich repeatPROSITEPS51450LRRcoord: 163..185
score: 5.61
IPR001611Leucine-rich repeatPROSITEPS51450LRRcoord: 115..136
score: 6.441
IPR001611Leucine-rich repeatPROSITEPS51450LRRcoord: 790..811
score: 6.688
IPR032675Leucine-rich repeat domain superfamilyGENE3DG3DSA:3.80.10.10coord: 263..482
e-value: 1.8E-54
score: 186.0
IPR032675Leucine-rich repeat domain superfamilyGENE3DG3DSA:3.80.10.10coord: 711..903
e-value: 6.0E-49
score: 167.9
IPR032675Leucine-rich repeat domain superfamilyGENE3DG3DSA:3.80.10.10coord: 37..255
e-value: 7.1E-50
score: 171.0
IPR032675Leucine-rich repeat domain superfamilyGENE3DG3DSA:3.80.10.10coord: 492..685
e-value: 1.4E-44
score: 153.5
IPR013032EGF-like, conserved sitePFAMPF12661hEGFcoord: 1400..1424
e-value: 0.021
score: 15.4
coord: 955..978
e-value: 0.029
score: 15.0
coord: 1073..1094
e-value: 0.0016
score: 19.0
coord: 995..1016
e-value: 1.9E-4
score: 21.9
IPR013032EGF-like, conserved sitePROSITEPS00022EGF_1coord: 1050..1061
IPR013032EGF-like, conserved sitePROSITEPS01186EGF_2coord: 932..943
IPR013032EGF-like, conserved sitePROSITEPS01186EGF_2coord: 1088..1099
IPR013032EGF-like, conserved sitePROSITEPS00022EGF_1coord: 932..943
IPR013032EGF-like, conserved sitePROSITEPS01186EGF_2coord: 972..983
IPR013032EGF-like, conserved sitePROSITEPS01186EGF_2coord: 1162..1173
IPR013032EGF-like, conserved sitePROSITEPS00022EGF_1coord: 972..983
IPR013032EGF-like, conserved sitePROSITEPS01186EGF_2coord: 1418..1429
IPR013032EGF-like, conserved sitePROSITEPS00022EGF_1coord: 1162..1173
IPR013032EGF-like, conserved sitePROSITEPS00022EGF_1coord: 1088..1099
IPR013032EGF-like, conserved sitePROSITEPS00022EGF_1coord: 1010..1021
IPR013032EGF-like, conserved sitePROSITEPS01186EGF_2coord: 1050..1061
IPR013032EGF-like, conserved sitePROSITEPS00022EGF_1coord: 1418..1429
IPR013032EGF-like, conserved sitePROSITEPS01186EGF_2coord: 1010..1021
IPR000152EGF-type aspartate/asparagine hydroxylation sitePROSITEPS00010ASX_HYDROXYLcoord: 1001..1012
IPR000152EGF-type aspartate/asparagine hydroxylation sitePROSITEPS00010ASX_HYDROXYLcoord: 1079..1090
IPR018097EGF-like calcium-binding, conserved sitePROSITEPS01187EGF_CAcoord: 1064..1088
IPR018097EGF-like calcium-binding, conserved sitePROSITEPS01187EGF_CAcoord: 986..1010
IPR013320Concanavalin A-like lectin/glucanase domain superfamilySUPERFAMILYSSF49899Concanavalin A-like lectins/glucanasescoord: 1158..1358