slit1
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30023930 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 15
Homology
BLAST of slit1 vs. Ensembl Human
Match: SLIT3 (slit guidance ligand 3 [Source:HGNC Symbol;Acc:HGNC:11087]) HSP 1 Score: 723.776 bits (1867), Expect = 0.000e+0 Identity = 464/1375 (33.75%), Postives = 701/1375 (50.98%), Query Frame = 1 Query: 157 CPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQLQ------------------------CINDNIFNKLGMLKYLLLEGNKIFWI-SEAISKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCASEDKANLENMCPSDKKVCSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQIYNFQC--NKEFESKYETHDEECVPKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLPPIYSIHEAPNLMKLDLS------------------------FNQIETLEPDVFTRSSKLREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQFHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCDGNASVYHSVDSCHETNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVLSFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGN-YECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYC-NDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNSIYF-QKNSYIEISPPSRGFLMPRGNISLNFMTNSSSGLILYQGSEQTYLVVNLYQGHLRISYNLGDKVVGPEG--YSMGMVNDSRLHALHIEIVGENLTMYLNGRVHCTLKSKYLNSNRYMALDTGIFIGGAPHKILQSAIMKFHIETDSEFIGCMESLLINRKKIDFSKYLPKSINVHPGC 4113 CP C C SV+C VP + RL+L N I I K DF L NL+VL L +N+++ IE GAF +L L +LRLN+N +Q LP+ LF KL +LDL +NQ+Q CI D F L L+ L L N I I + + + ++ LR+H N L C+CHLAWL+ +L+Q+ + LC P L ++ + E+ C + C ++ C C CS++ IV C GK L IP +LP +VE+ L+ N+I + G ++YK LK+I +++N + DI P AF GL LTS+ L N + I K F GLVSL L L N I CL +T + + L+LL L +NKL ++ K F P +++ L+L NP CDC + W+ DY++D T A C PR L + ++ F+C ++++ S++ + EC + CP KC C +V+CSN L+ +P + L ++ N + L + PNL K++LS NQ+ET+ VF S L+ + L SN + C++N+TF L + +L L +N I+ I +F ++L + L N F+C+C L W+ +W R V GN P CQ P LK+ PI + + F CDGN SC + C+ T V CS+ L+ +P +PKD TELYL+ NHL ++ L +L + LS N I+ L + + L TL+LS+N+L+CI AF L +L+VL L ND+S++P +FNDL +L+++ALG NP HCDC+++WL+++ + + GI+ C+SPE M + + + F C D I AKC++C+ PCK+NG C+ Y+C CPY + GK+C IN C PC++GG C D++ + + C CP+GF G +C IN DDC N C N ATC D N+Y C C + G+ CD +C ++N CQ+ +C L + + C C G+ GK CE + DDC + C+H +QCVD N Y C C G+ G FCE P P VL + S C +EC N CI Q+ C+C PG++G C+ L ++ F K+SY+E+ + + P+ NISL T+ +G++LY+G + L + LYQGH+R+ Y D + P YS+ VND + H++ + + + L + ++ +L L + +++ +++GG P SA+ + F GC+ + IN + DF P+S+ V PGC Sbjct: 34 CPTKCTCSAASVDCHGLGLRAVPRGIPRNAERLDLDRNNITRITKMDFAGLKNLRVLHLEDNQVSVIERGAFQDLKQLERLRLNKNKLQVLPELLFQSTPKLTRLDLSENQIQGIPRKAFRGITDVKNLQLDNNHISCIEDGAFRALRDLEILTLNNNNISRILVTSFNHMPKIRTLRLHSNHLYCDCHLAWLSDWLRQRRTV----GQFTLCMAPVHLRGFNVADVQKKEYVCPAPHSE--PPSCNANSISCPSPCTCSNN-IVDCRGKGLMEIPANLPEGIVEIRLEQNSIKAIPAGAFTQYKKLKRIDISKNQISDIAPDAFQGLKSLTSLVLYGNKITEIVKGLFDGLVSLQLLLLNANKINCLRVNTFQDLQNLNLLSLYDNKLQTISKGLFAPLQSIQTLHLAQNPFVCDCHLKWLADYLQDNPIETSGARCSSPRRLANKRISQIKSKKFRCSGSEDYRSRFSS---ECFMDLVCPEKCRCEGTIVDCSNQKLVRIPSHLPEYVTDLRLNDNEVSVLEATGIFKKLPNLRKINLSNNKIKEVREGAFDGAASVQELMLTGNQLETVHGRVFRGLSGLKTLMLRSNLIGCVSNDTFAGLSSVRLLSLYDNRITTITPGAFTTLVSLSTINLLSNPFNCNCHLAWLGKWLRKRRIVSGN---PRCQKPFFLKEIPIQDVAIQDFTCDGNEE-----SSCQLSPRCPEQCTC-----------------METVVRCSNKGLRALPRGMPKDVTELYLEGNHLTAVPR-ELSALRHLTLIDLSNNSISMLTNYTFSNMSHLSTLILSYNRLRCIPVHAFNGLRSLRVLTLHGNDISSVPEGSFNDLTSLSHLALGTNPLHCDCSLRWLSEWVKAGYKEPGIARCSSPEPMADRLLLTTPTHRFQCKGPV-DINIVAKCNACLSSPCKNNGTCTQDPVELYRCACPYSYKGKDCTVPINTCIQNPCQHGGTCHLSDSHKDGFSCSCPLGFEGQRCEINPDDCEDNDCENNATCVDGINNYVCICPPNYTGELCDEVIDHCVPELNLCQHEAKCIPL-DKGFSCECVPGYSGKLCETDNDDCVAHKCRHGAQCVDTINGYTCTCPQGFSGPFCEHPP--PMVL-----------------------LQTSPCDQYECQNGAQCI----VVQQEPTCRCPPGFAGPRCEKLITVNFVGKDSYVEL---ASAKVRPQANISLQVATDKDNGILLYKG-DNDPLALELYQGHVRLVY---DSLSSPPTTVYSVETVNDGQFHSVELVTLNQTLNLVVDKGTPKSLGK--LQKQPAVGINSPLYLGGIPTSTGLSALRQGTDRPLGGFHGCIHEVRINNELQDFKALPPQSLGVSPGC 1332 HSP 2 Score: 195.282 bits (495), Expect = 2.377e-49 Identity = 140/469 (29.85%), Postives = 229/469 (48.83%), Query Frame = 1 Query: 154 ICPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSD-FLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQLQCINDNIFNKLGMLKYLLLEGNKIFWISE-AISKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCASEDKANLENMCPSDKKVCSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQIYNFQCNKEFESKYETHDEECVPKVSCPLKCTCSSDVV 1554 +CP C+CE V+C ++ +P L E T L L N ++V+ + F KL NL+ + LSNN+I + GAFD ++ +L L N ++ + +F L+ L+ L LR N + C++++ F L ++ L L N+I I+ A + L L + + N NCNCHLAWL +L+++ + + + C PF L IPI ++ +F C N E+ C + C ++C C + +V+C K L +P+ +P +V EL L+ N++T + + LS + L I L+ N + + F ++ L+++ L+ N L+ I +AF GL SL L L NDI +P+ + N LTSL +L L NPL+CDC + W+ +++K +A C P + L + FQC + C+ C TC+ D V Sbjct: 504 VCPEKCRCEGTIVDCSNQKLVRIPSHLPEYVTDLRLNDNEVSVLEATGIFKKLPNLRKINLSNNKIKEVREGAFDGAASVQELMLTGNQLETVHGRVFRGLSGLKTLMLRSNLIGCVSNDTFAGLSSVRLLSLYDNRITTITPGAFTTLVSLSTINLLSNPFNCNCHLAWLGKWLRKRRIV----SGNPRCQKPFFLKEIPIQDVAIQDFTC----DGNEESSCQLSPR-CPEQCTC-METVVRCSNKGLRALPRGMPKDVTELYLEGNHLTAVPR-ELSALRHLTLIDLSNNSISMLTNYTFSNMSHLSTLILSYNRLRCIPVHAFNGLRSLRVLTLHGNDISSVPEGSF-------------NDLTSLS-----------HLALGTNPLHCDCSLRWLSEWVKAGYKEPGIARCSSPEPMADRLLLTTPTHRFQCKGPVDINIVAKCNACL-SSPCKNNGTCTQDPV 936 HSP 3 Score: 182.956 bits (463), Expect = 1.433e-45 Identity = 128/479 (26.72%), Postives = 213/479 (44.47%), Query Frame = 1 Query: 1507 PKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLP-----------------------------------------------PIYSIHEAPNLMKLDLSFNQIETLEPDVFTRSSKLREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQFHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCDGNASVYHSVDSCHETNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVL------------------------SFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICS 2730 P V+CP KCTCS+ V+C GL ++P I + + L + NN+ ++ P P L +LDLS NQI+ + F + ++ + L++N + CI + F+ L++LEIL L+ N IS I+ SF + ++ L L+ N +CDC L W+S+W R TV + C +P+ L+ + + K ++C S S ++ + S + C+ N VDC L EIP +P+ E+ L++N ++++ +F LK + +S N I+++ + LKSL +LVL + NK+ C+ + F++L NL +L L N + TI F L+++ + L QNPF CDC++KWL + D ++ + C+SP ++ K I K F CS Sbjct: 30 PAVACPTKCTCSAASVDCHGLGLRAVPRGIPRNAERLDLDRNNITRITKMDFAGLKNLRVLHLEDNQVSVIERGAFQDLKQLERLRLNKNKLQVLPELLFQSTPKLTRLDLSENQIQGIPRKAFRGITDVKNLQLDNNHISCIEDGAFRALRDLEILTLNNNNISRILVTSFNHMPKIRTLRLHSNHLYCDCHLAWLSDWLRQRRTV---GQFTLCMAPVHLRGFNVADVQKKEYVCPAPHSEPPSCNA--NSISCPSPCTCSN-----------------NIVDCRGKGLMEIPANLPEGIVEIRLEQNSIKAIPAGAFTQYKKLKRIDISKNQISDIAPDAFQGLKSLTSLVLYGNKITEIVKGLFDGLVSLQLLLLNANKINCLRVNTFQDLQNLNLLSLYDNKLQTISKGLFAPLQSIQTLHLAQNPFVCDCHLKWLADYLQDNPIETSGARCSSPRRLANKRISQIKSKKFRCS 486 HSP 4 Score: 53.5286 bits (127), Expect = 4.651e-6 Identity = 36/111 (32.43%), Postives = 55/111 (49.55%), Query Frame = 1 Query: 2917 GICKNIDNYGNYECVCPVGFRGPKCTINV-DDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCND-INPCQ----NSGQCQLLPNNDYKCVCSKGWEGKQCER 3231 G+C++++ + C C G+ GP C D C+G+ C +G C SY CKC G+ G CD K ND N C + GQC + + C+C G+ G+ C++ Sbjct: 1341 GLCRSVEK-DSVVCECRPGWTGPLCDQEARDPCLGHRCHHGK-CVATGTSYMCKCAEGYGGDLCDNK----NDSANACSAFKCHHGQCHISDQGEPYCLCQPGFSGEHCQQ 1445
BLAST of slit1 vs. Ensembl Human
Match: SLIT3 (slit guidance ligand 3 [Source:HGNC Symbol;Acc:HGNC:11087]) HSP 1 Score: 720.694 bits (1859), Expect = 0.000e+0 Identity = 464/1381 (33.60%), Postives = 701/1381 (50.76%), Query Frame = 1 Query: 157 CPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQLQ------------------------CINDNIFNKLGMLKYLLLEGNKIFWI-SEAISKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCASEDKANLENMCPSDKKVCSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQIYNFQC--NKEFESKYETHDEECVPKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLPPIYSIHEAPNLMKLDLS------------------------FNQIETLEPDVFTRSSKLREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQFHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCDGNASVYHSVDSCHETNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVLSFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDF------ICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGN-YECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYC-NDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNSIYF-QKNSYIEISPPSRGFLMPRGNISLNFMTNSSSGLILYQGSEQTYLVVNLYQGHLRISYNLGDKVVGPEG--YSMGMVNDSRLHALHIEIVGENLTMYLNGRVHCTLKSKYLNSNRYMALDTGIFIGGAPHKILQSAIMKFHIETDSEFIGCMESLLINRKKIDFSKYLPKSINVHPGC 4113 CP C C SV+C VP + RL+L N I I K DF L NL+VL L +N+++ IE GAF +L L +LRLN+N +Q LP+ LF KL +LDL +NQ+Q CI D F L L+ L L N I I + + + ++ LR+H N L C+CHLAWL+ +L+Q+ + LC P L ++ + E+ C + C ++ C C CS++ IV C GK L IP +LP +VE+ L+ N+I + G ++YK LK+I +++N + DI P AF GL LTS+ L N + I K F GLVSL L L N I CL +T + + L+LL L +NKL ++ K F P +++ L+L NP CDC + W+ DY++D T A C PR L + ++ F+C ++++ S++ + EC + CP KC C +V+CSN L+ +P + L ++ N + L + PNL K++LS NQ+ET+ VF S L+ + L SN + C++N+TF L + +L L +N I+ I +F ++L + L N F+C+C L W+ +W R V GN P CQ P LK+ PI + + F CDGN SC + C+ T V CS+ L+ +P +PKD TELYL+ NHL ++ L +L + LS N I+ L + + L TL+LS+N+L+CI AF L +L+VL L ND+S++P +FNDL +L+++ALG NP HCDC+++WL+++ + + GI+ C+SPE M + + + F C D I AKC++C+ PCK+NG C+ Y+C CPY + GK+C IN C PC++GG C D++ + + C CP+GF G +C IN DDC N C N ATC D N+Y C C + G+ CD +C ++N CQ+ +C L + + C C G+ GK CE + DDC + C+H +QCVD N Y C C G+ G FCE P P VL + S C +EC N CI Q+ C+C PG++G C+ L ++ F K+SY+E+ + + P+ NISL T+ +G++LY+G + L + LYQGH+R+ Y D + P YS+ VND + H++ + + + L + ++ +L L + +++ +++GG P SA+ + F GC+ + IN + DF P+S+ V PGC Sbjct: 34 CPTKCTCSAASVDCHGLGLRAVPRGIPRNAERLDLDRNNITRITKMDFAGLKNLRVLHLEDNQVSVIERGAFQDLKQLERLRLNKNKLQVLPELLFQSTPKLTRLDLSENQIQGIPRKAFRGITDVKNLQLDNNHISCIEDGAFRALRDLEILTLNNNNISRILVTSFNHMPKIRTLRLHSNHLYCDCHLAWLSDWLRQRRTV----GQFTLCMAPVHLRGFNVADVQKKEYVCPAPHSE--PPSCNANSISCPSPCTCSNN-IVDCRGKGLMEIPANLPEGIVEIRLEQNSIKAIPAGAFTQYKKLKRIDISKNQISDIAPDAFQGLKSLTSLVLYGNKITEIVKGLFDGLVSLQLLLLNANKINCLRVNTFQDLQNLNLLSLYDNKLQTISKGLFAPLQSIQTLHLAQNPFVCDCHLKWLADYLQDNPIETSGARCSSPRRLANKRISQIKSKKFRCSGSEDYRSRFSS---ECFMDLVCPEKCRCEGTIVDCSNQKLVRIPSHLPEYVTDLRLNDNEVSVLEATGIFKKLPNLRKINLSNNKIKEVREGAFDGAASVQELMLTGNQLETVHGRVFRGLSGLKTLMLRSNLIGCVSNDTFAGLSSVRLLSLYDNRITTITPGAFTTLVSLSTINLLSNPFNCNCHLAWLGKWLRKRRIVSGN---PRCQKPFFLKEIPIQDVAIQDFTCDGNEE-----SSCQLSPRCPEQCTC-----------------METVVRCSNKGLRALPRGMPKDVTELYLEGNHLTAVPR-ELSALRHLTLIDLSNNSISMLTNYTFSNMSHLSTLILSYNRLRCIPVHAFNGLRSLRVLTLHGNDISSVPEGSFNDLTSLSHLALGTNPLHCDCSLRWLSEWVKAGYKEPGIARCSSPEPMADRLLLTTPTHRFQCKVLWFCCPGPVDINIVAKCNACLSSPCKNNGTCTQDPVELYRCACPYSYKGKDCTVPINTCIQNPCQHGGTCHLSDSHKDGFSCSCPLGFEGQRCEINPDDCEDNDCENNATCVDGINNYVCICPPNYTGELCDEVIDHCVPELNLCQHEAKCIPL-DKGFSCECVPGYSGKLCETDNDDCVAHKCRHGAQCVDTINGYTCTCPQGFSGPFCEHPP--PMVL-----------------------LQTSPCDQYECQNGAQCI----VVQQEPTCRCPPGFAGPRCEKLITVNFVGKDSYVEL---ASAKVRPQANISLQVATDKDNGILLYKG-DNDPLALELYQGHVRLVY---DSLSSPPTTVYSVETVNDGQFHSVELVTLNQTLNLVVDKGTPKSLGK--LQKQPAVGINSPLYLGGIPTSTGLSALRQGTDRPLGGFHGCIHEVRINNELQDFKALPPQSLGVSPGC 1339 HSP 2 Score: 194.897 bits (494), Expect = 2.959e-49 Identity = 134/437 (30.66%), Postives = 220/437 (50.34%), Query Frame = 1 Query: 154 ICPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSD-FLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQLQCINDNIFNKLGMLKYLLLEGNKIFWISE-AISKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCASEDKANLENMCPSDKKVCSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQIYNFQC 1458 +CP C+CE V+C ++ +P L E T L L N ++V+ + F KL NL+ + LSNN+I + GAFD ++ +L L N ++ + +F L+ L+ L LR N + C++++ F L ++ L L N+I I+ A + L L + + N NCNCHLAWL +L+++ + + + C PF L IPI ++ +F C N E+ C + C ++C C + +V+C K L +P+ +P +V EL L+ N++T + + LS + L I L+ N + + F ++ L+++ L+ N L+ I +AF GL SL L L NDI +P+ + N LTSL +L L NPL+CDC + W+ +++K +A C P + L + FQC Sbjct: 504 VCPEKCRCEGTIVDCSNQKLVRIPSHLPEYVTDLRLNDNEVSVLEATGIFKKLPNLRKINLSNNKIKEVREGAFDGAASVQELMLTGNQLETVHGRVFRGLSGLKTLMLRSNLIGCVSNDTFAGLSSVRLLSLYDNRITTITPGAFTTLVSLSTINLLSNPFNCNCHLAWLGKWLRKRRIV----SGNPRCQKPFFLKEIPIQDVAIQDFTC----DGNEESSCQLSPR-CPEQCTC-METVVRCSNKGLRALPRGMPKDVTELYLEGNHLTAVPR-ELSALRHLTLIDLSNNSISMLTNYTFSNMSHLSTLILSYNRLRCIPVHAFNGLRSLRVLTLHGNDISSVPEGSF-------------NDLTSLS-----------HLALGTNPLHCDCSLRWLSEWVKAGYKEPGIARCSSPEPMADRLLLTTPTHRFQC 905 HSP 3 Score: 182.956 bits (463), Expect = 1.556e-45 Identity = 128/479 (26.72%), Postives = 213/479 (44.47%), Query Frame = 1 Query: 1507 PKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLP-----------------------------------------------PIYSIHEAPNLMKLDLSFNQIETLEPDVFTRSSKLREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQFHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCDGNASVYHSVDSCHETNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVL------------------------SFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICS 2730 P V+CP KCTCS+ V+C GL ++P I + + L + NN+ ++ P P L +LDLS NQI+ + F + ++ + L++N + CI + F+ L++LEIL L+ N IS I+ SF + ++ L L+ N +CDC L W+S+W R TV + C +P+ L+ + + K ++C S S ++ + S + C+ N VDC L EIP +P+ E+ L++N ++++ +F LK + +S N I+++ + LKSL +LVL + NK+ C+ + F++L NL +L L N + TI F L+++ + L QNPF CDC++KWL + D ++ + C+SP ++ K I K F CS Sbjct: 30 PAVACPTKCTCSAASVDCHGLGLRAVPRGIPRNAERLDLDRNNITRITKMDFAGLKNLRVLHLEDNQVSVIERGAFQDLKQLERLRLNKNKLQVLPELLFQSTPKLTRLDLSENQIQGIPRKAFRGITDVKNLQLDNNHISCIEDGAFRALRDLEILTLNNNNISRILVTSFNHMPKIRTLRLHSNHLYCDCHLAWLSDWLRQRRTV---GQFTLCMAPVHLRGFNVADVQKKEYVCPAPHSEPPSCNA--NSISCPSPCTCSN-----------------NIVDCRGKGLMEIPANLPEGIVEIRLEQNSIKAIPAGAFTQYKKLKRIDISKNQISDIAPDAFQGLKSLTSLVLYGNKITEIVKGLFDGLVSLQLLLLNANKINCLRVNTFQDLQNLNLLSLYDNKLQTISKGLFAPLQSIQTLHLAQNPFVCDCHLKWLADYLQDNPIETSGARCSSPRRLANKRISQIKSKKFRCS 486 HSP 4 Score: 53.1434 bits (126), Expect = 5.372e-6 Identity = 36/111 (32.43%), Postives = 55/111 (49.55%), Query Frame = 1 Query: 2917 GICKNIDNYGNYECVCPVGFRGPKCTINV-DDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCND-INPCQ----NSGQCQLLPNNDYKCVCSKGWEGKQCER 3231 G+C++++ + C C G+ GP C D C+G+ C +G C SY CKC G+ G CD K ND N C + GQC + + C+C G+ G+ C++ Sbjct: 1348 GLCRSVEK-DSVVCECRPGWTGPLCDQEARDPCLGHRCHHGK-CVATGTSYMCKCAEGYGGDLCDNK----NDSANACSAFKCHHGQCHISDQGEPYCLCQPGFSGEHCQQ 1452
BLAST of slit1 vs. Ensembl Human
Match: SLIT2 (slit guidance ligand 2 [Source:HGNC Symbol;Acc:HGNC:11086]) HSP 1 Score: 714.146 bits (1842), Expect = 0.000e+0 Identity = 487/1483 (32.84%), Postives = 735/1483 (49.56%), Query Frame = 1 Query: 151 EICPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQLQ------------------------CINDNIFNKLGMLKYLLLEGNKIFWISEA-ISKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCASEDKANLENMCPSDKKV-CSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQIYNFQCN--KEFESKYETHDEECVPKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLPPIYSIHEAPNLMKLDLSFNQIETLEPDVFTRSSKLREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQ------------------------FHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCD-GNASVYHSVDSCHETNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVLSFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGIC--KNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYC-NDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNSIYF-QKNSYIEISPPSRGFLMPRGNISLNFMTNSSSGLILYQGSEQTYLVVNLYQGHLRISYNLGDKVVGPEGYSMGMVNDSRLHALHIEIVGENLTMYLNGRVHCTLKSKYLNSNRYMALDTGIFIGGAPHKILQSAIMKFHIETDSEFIGCMESLLINRKKIDFSKYLPKSINVHPGCGKLISTVVASDLKIPSIMXXXXXXXXXXXXXXXNNVCKRPGICKNQGVCEVLPGSKKKFRCICKNGFVGRKCKKRKSMCHGTFTESHIVDPDSQGTCKSIERFQY 4428 + CP C C +V+C +VP + T RL+L GN I I K+DF L +L+VLQL N+I++IE GAF +L L +LRLNRN +Q P+ LF KL +LDL +NQ+Q CI D F L L+ L L N I +S A + + L+ R+H N L C+CHLAWL+ +L+Q+ + G ++ C P L ++ + EF C+ E++ + M PS + C C CS++ IV C GK L+ IP +LP + E+ L+ N I ++ G S YK L++I L+ N + ++ P AF GL L S+ L N + + K+ F GL SL L L N I CL D + L+LL L +NKL ++ K TF P ++ ++L NP CDC + W+ DY+ T A C PR L + ++ F+C+ +++ SK +C ++CP KC C V+CSN L +PE I T L +++N L + P L K++ S N+I +E F +S + EI L SNRL+ + ++ FK L+ L+ L L N I+C+ DSF +++ L L NQ F+C+C L W+ EW R V GN P CQ P LK+ PI + + F CD GN +SC + T C+ T V CS+ LK +P IP+D TELYLD N +L N +L + LS N I+ L+ + + L TL+LS+N+L+CI F L +L++L L ND+S +P AFNDL L+++A+G NP +CDCN++WL+ + + + GI+ CA P +M K + + F C D I AKC+ C+ PCK++G C+ Y+C CPY F G++CD I+AC PC++GG C K + G + C+C GF G C +NVDDC N C N +TC D N+Y+C C + G+ C+ K +C D+NPCQ+ +C L P +KC C+ G+ G+ C+ + DDC+ N CK+ + C D N Y C+C GY G FCE P VL S C +C N CI + + C+C PGY G C+ L S+ F K SY++I P ++ + P+ NI+L T+ SG++LY+G ++ ++ V LY+G +R SY+ G YS+ +ND H + + + ++L++ ++G + + L+ + D+ +++GG P K +++ + + + F GC+ +L IN + DF K +P + PGC V A G C+ P S+ F C C+ G++G C +R + + + + GTC I F Y Sbjct: 26 QACPAQCSCSGSTVDCHGLALRSVPRNIPRNTERLDLNGNNITRITKTDFAGLRHLRVLQLMENKISTIERGAFQDLKELERLRLNRNHLQLFPELLFLGTAKLYRLDLSENQIQAIPRKAFRGAVDIKNLQLDYNQISCIEDGAFRALRDLEVLTLNNNNITRLSVASFNHMPKLRTFRLHSNNLYCDCHLAWLSDWLRQRPRV---GLYTQ-CMGPSHLRGHNVAEVQKREFVCSDEEEGHQSFMAPSCSVLHCPAACTCSNN-IVDCRGKGLTEIPTNLPETITEIRLEQNTIKVIPPGAFSPYKKLRRIDLSNNQISELAPDAFQGLRSLNSLVLYGNKITELPKSLFEGLFSLQLLLLNANKINCLRVDAFQDLHNLNLLSLYDNKLQTIAKGTFSPLRAIQTMHLAQNPFICDCHLKWLADYLHTNPIETSGARCTSPRRLANKRIGQIKSKKFRCSGTEDYRSKLSG---DCFADLACPEKCRCEGTTVDCSNQKLNKIPEHIPQYTAELRLNNNEFTVLEATGIFKKLPQLRKINFSNNKITDIEEGAFEGASGVNEILLTSNRLENVQHKMFKGLESLKTLMLRSNRITCVGNDSFIGLSSVRLLSLYDNQITTVAPGAFDTLHSLSTLNLLANPFNCNCYLAWLGEWLRKKRIVTGN---PRCQKPYFLKEIPIQDVAIQDFTCDDGNDD-----NSCSPLSRCPTECTC-----------------LDTVVRCSNKGLKVLPKGIPRDVTELYLDGNQF-TLVPKELSNYKHLTLIDLSNNRISTLSNQSFSNMTQLLTLILSYNRLRCIPPRTFDGLKSLRLLSLHGNDISVVPEGAFNDLSALSHLAIGANPLYCDCNMQWLSDWVKSEYKEPGIARCAGPGEMADKLLLTTPSKKFTCQGPV-DVNILAKCNPCLSNPCKNDGTCNSDPVDFYRCTCPYGFKGQDCDVPIHACISNPCKHGGTCHLKEGEEDG-FWCICADGFEGENCEVNVDDCEDNDCENNSTCVDGINNYTCLCPPEYTGELCEEKLDFCAQDLNPCQHDSKCILTPKG-FKCDCTPGYVGEHCDIDFDDCQDNKCKNGAHCTDAVNGYTCICPEGYSGLFCEFSP--PMVLP-----------------------RTSPCDNFDCQNGAQCI----VRINEPICQCLPGYQGEKCEKLVSVNFINKESYLQI-PSAK--VRPQTNITLQIATDEDSGILLYKG-DKDHIAVELYRGRVRASYDTGSHPASAI-YSVETINDGNFHIVELLALDQSLSLSVDGGNPKIITN--LSKQSTLNFDSPLYVGGMPGKSNVASLRQAPGQNGTSFHGCIRNLYINSELQDFQK-VPMQTGILPGCEPCHKKVCA------------------------------------HGTCQ--PSSQAGFTCECQEGWMGPLCDQRTN-------DPCLGNKCVHGTCLPINAFSY 1389 HSP 2 Score: 58.9214 bits (141), Expect = 1.038e-7 Identity = 43/111 (38.74%), Postives = 56/111 (50.45%), Query Frame = 1 Query: 2917 GICKNIDNYGNYECVCPVGFRGPKCTINVDD-CVGNLCVNGATCNDLNN-SYSCKCQSGFRGKYCDIKFLYCNDINPCQ----NSGQCQLLPNNDYKCVCSKGWEGKQCER 3231 G C+ G + C C G+ GP C +D C+GN CV+G TC +N SYSCKC G G CD + + NPCQ G+C+L C CS G+ G C+R Sbjct: 1340 GTCQPSSQAG-FTCECQEGWMGPLCDQRTNDPCLGNKCVHG-TCLPINAFSYSCKCLEGHGGVLCDEEE---DLFNPCQAIKCKHGKCRLSGLGQPYCECSSGYTGDSCDR 1445
BLAST of slit1 vs. Ensembl Human
Match: SLIT2 (slit guidance ligand 2 [Source:HGNC Symbol;Acc:HGNC:11086]) HSP 1 Score: 705.671 bits (1820), Expect = 0.000e+0 Identity = 486/1483 (32.77%), Postives = 731/1483 (49.29%), Query Frame = 1 Query: 151 EICPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQLQ------------------------CINDNIFNKLGMLKYLLLEGNKIFWISEA-ISKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCASEDKANLENMCPSDKKV-CSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQIYNFQCN--KEFESKYETHDEECVPKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLPPIYSIHEAPNLMKLDLSFNQIETLEPDVFTRSSKLREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQ------------------------FHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCD-GNASVYHSVDSCHETNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVLSFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGIC--KNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYC-NDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNSIYF-QKNSYIEISPPSRGFLMPRGNISLNFMTNSSSGLILYQGSEQTYLVVNLYQGHLRISYNLGDKVVGPEGYSMGMVNDSRLHALHIEIVGENLTMYLNGRVHCTLKSKYLNSNRYMALDTGIFIGGAPHKILQSAIMKFHIETDSEFIGCMESLLINRKKIDFSKYLPKSINVHPGCGKLISTVVASDLKIPSIMXXXXXXXXXXXXXXXNNVCKRPGICKNQGVCEVLPGSKKKFRCICKNGFVGRKCKKRKSMCHGTFTESHIVDPDSQGTCKSIERFQY 4428 + CP C C +V+C +VP + T RL+L GN I I K+DF L +L+VLQL N+I++IE GAF +L L +LRLNRN +Q P+ LF KL +LDL +NQ+Q CI D F L L+ L L N I +S A + + L+ R+H N L C+CHLAWL+ +L+Q+ + G ++ C P L ++ + EF C+ M PS + C C CS++ IV C GK L+ IP +LP + E+ L+ N I ++ G S YK L++I L+ N + ++ P AF GL L S+ L N + + K+ F GL SL L L N I CL D + L+LL L +NKL ++ K TF P ++ ++L NP CDC + W+ DY+ T A C PR L + ++ F+C+ +++ SK +C ++CP KC C V+CSN L +PE I T L +++N L + P L K++ S N+I +E F +S + EI L SNRL+ + ++ FK L+ L+ L L N I+C+ DSF +++ L L NQ F+C+C L W+ EW R V GN P CQ P LK+ PI + + F CD GN +SC + T C+ T V CS+ LK +P IP+D TELYLD N +L N +L + LS N I+ L+ + + L TL+LS+N+L+CI F L +L++L L ND+S +P AFNDL L+++A+G NP +CDCN++WL+ + + + GI+ CA P +M K + + F C D I AKC+ C+ PCK++G C+ Y+C CPY F G++CD I+AC PC++GG C K + G + C+C GF G C +NVDDC N C N +TC D N+Y+C C + G+ C+ K +C D+NPCQ+ +C L P +KC C+ G+ G+ C+ + DDC+ N CK+ + C D N Y C+C GY G FCE P VL S C +C N CI + + C+C PGY G C+ L S+ F K SY++I P ++ + P+ NI+L T+ SG++LY+G ++ ++ V LY+G +R SY+ G YS+ +ND H + + + ++L++ ++G + + L+ + D+ +++GG P K +++ + + + F GC+ +L IN + DF K +P + PGC V A G C+ P S+ F C C+ G++G C +R + + + + GTC I F Y Sbjct: 26 QACPAQCSCSGSTVDCHGLALRSVPRNIPRNTERLDLNGNNITRITKTDFAGLRHLRVLQLMENKISTIERGAFQDLKELERLRLNRNHLQLFPELLFLGTAKLYRLDLSENQIQAIPRKAFRGAVDIKNLQLDYNQISCIEDGAFRALRDLEVLTLNNNNITRLSVASFNHMPKLRTFRLHSNNLYCDCHLAWLSDWLRQRPRV---GLYTQ-CMGPSHLRGHNVAEVQKREFVCSGHQSF----MAPSCSVLHCPAACTCSNN-IVDCRGKGLTEIPTNLPETITEIRLEQNTIKVIPPGAFSPYKKLRRIDLSNNQISELAPDAFQGLRSLNSLVLYGNKITELPKSLFEGLFSLQLLLLNANKINCLRVDAFQDLHNLNLLSLYDNKLQTIAKGTFSPLRAIQTMHLAQNPFICDCHLKWLADYLHTNPIETSGARCTSPRRLANKRIGQIKSKKFRCSGTEDYRSKLSG---DCFADLACPEKCRCEGTTVDCSNQKLNKIPEHIPQYTAELRLNNNEFTVLEATGIFKKLPQLRKINFSNNKITDIEEGAFEGASGVNEILLTSNRLENVQHKMFKGLESLKTLMLRSNRITCVGNDSFIGLSSVRLLSLYDNQITTVAPGAFDTLHSLSTLNLLANPFNCNCYLAWLGEWLRKKRIVTGN---PRCQKPYFLKEIPIQDVAIQDFTCDDGNDD-----NSCSPLSRCPTECTC-----------------LDTVVRCSNKGLKVLPKGIPRDVTELYLDGNQF-TLVPKELSNYKHLTLIDLSNNRISTLSNQSFSNMTQLLTLILSYNRLRCIPPRTFDGLKSLRLLSLHGNDISVVPEGAFNDLSALSHLAIGANPLYCDCNMQWLSDWVKSEYKEPGIARCAGPGEMADKLLLTTPSKKFTCQGPV-DVNILAKCNPCLSNPCKNDGTCNSDPVDFYRCTCPYGFKGQDCDVPIHACISNPCKHGGTCHLKEGEEDG-FWCICADGFEGENCEVNVDDCEDNDCENNSTCVDGINNYTCLCPPEYTGELCEEKLDFCAQDLNPCQHDSKCILTPKG-FKCDCTPGYVGEHCDIDFDDCQDNKCKNGAHCTDAVNGYTCICPEGYSGLFCEFSP--PMVLP-----------------------RTSPCDNFDCQNGAQCI----VRINEPICQCLPGYQGEKCEKLVSVNFINKESYLQI-PSAK--VRPQTNITLQIATDEDSGILLYKG-DKDHIAVELYRGRVRASYDTGSHPASAI-YSVETINDGNFHIVELLALDQSLSLSVDGGNPKIITN--LSKQSTLNFDSPLYVGGMPGKSNVASLRQAPGQNGTSFHGCIRNLYINSELQDFQK-VPMQTGILPGCEPCHKKVCA------------------------------------HGTCQ--PSSQAGFTCECQEGWMGPLCDQRTN-------DPCLGNKCVHGTCLPINAFSY 1385 HSP 2 Score: 58.9214 bits (141), Expect = 1.029e-7 Identity = 43/111 (38.74%), Postives = 56/111 (50.45%), Query Frame = 1 Query: 2917 GICKNIDNYGNYECVCPVGFRGPKCTINVDD-CVGNLCVNGATCNDLNN-SYSCKCQSGFRGKYCDIKFLYCNDINPCQ----NSGQCQLLPNNDYKCVCSKGWEGKQCER 3231 G C+ G + C C G+ GP C +D C+GN CV+G TC +N SYSCKC G G CD + + NPCQ G+C+L C CS G+ G C+R Sbjct: 1336 GTCQPSSQAG-FTCECQEGWMGPLCDQRTNDPCLGNKCVHG-TCLPINAFSYSCKCLEGHGGVLCDEEE---DLFNPCQAIKCKHGKCRLSGLGQPYCECSSGYTGDSCDR 1441
BLAST of slit1 vs. Ensembl Human
Match: SLIT2 (slit guidance ligand 2 [Source:HGNC Symbol;Acc:HGNC:11086]) HSP 1 Score: 704.131 bits (1816), Expect = 0.000e+0 Identity = 487/1500 (32.47%), Postives = 736/1500 (49.07%), Query Frame = 1 Query: 151 EICPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQLQ------------------------CINDNIFNKLGMLKYLLLEGNKIFWISEA-ISKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCASEDKANLENMCPSDKKV-CSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQIYNFQCN----------KEFESKYETHDEECVPKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLPPIYSIHEAPNLMKLDLSFNQIETLEPDVFTRSSKLREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQ------------------------FHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCD-GNASVYHSVDSCHETNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVLSFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGIC--KNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGF---------RGKYCDIKFLYC-NDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNSIYF-QKNSYIEISPPSRGFLMPRGNISLNFMTNSSSGLILYQGSEQTYLVVNLYQGHLRISYNLGDKVVGPEGYSMGMVNDSRLHALHIEIVGENLTMYLNGRVHCTLKSKYLNSNRYMALDTGIFIGGAPHKILQSAIMKFHIETDSEFIGCMESLLINRKKIDFSKYLPKSINVHPGCGKLISTVVASDLKIPSIMXXXXXXXXXXXXXXXNNVCKRPGICKNQGVCEVLPGSKKKFRCICKNGFVGRKCKKRKSMCHGTFTESHIVDPDSQGTCKSIERFQY 4428 + CP C C +V+C +VP + T RL+L GN I I K+DF L +L+VLQL N+I++IE GAF +L L +LRLNRN +Q P+ LF KL +LDL +NQ+Q CI D F L L+ L L N I +S A + + L+ R+H N L C+CHLAWL+ +L+Q+ + G ++ C P L ++ + EF C+ E++ + M PS + C C CS++ IV C GK L+ IP +LP + E+ L+ N I ++ G S YK L++I L+ N + ++ P AF GL L S+ L N + + K+ F GL SL L L N I CL D + L+LL L +NKL ++ K TF P ++ ++L NP CDC + W+ DY+ T A C PR L + ++ F+C+ +++ SK +C ++CP KC C V+CSN L +PE I T L +++N L + P L K++ S N+I +E F +S + EI L SNRL+ + ++ FK L+ L+ L L N I+C+ DSF +++ L L NQ F+C+C L W+ EW R V GN P CQ P LK+ PI + + F CD GN +SC + T C+ T V CS+ LK +P IP+D TELYLD N +L N +L + LS N I+ L+ + + L TL+LS+N+L+CI F L +L++L L ND+S +P AFNDL L+++A+G NP +CDCN++WL+ + + + GI+ CA P +M K + + F C D I AKC+ C+ PCK++G C+ Y+C CPY F G++CD I+AC PC++GG C K + G + C+C GF G C +NVDDC N C N +TC D N+Y+C C + +G+ C+ K +C D+NPCQ+ +C L P +KC C+ G+ G+ C+ + DDC+ N CK+ + C D N Y C+C GY G FCE P VL S C +C N CI + + C+C PGY G C+ L S+ F K SY++I P ++ + P+ NI+L T+ SG++LY+G ++ ++ V LY+G +R SY+ G YS+ +ND H + + + ++L++ ++G + + L+ + D+ +++GG P K +++ + + + F GC+ +L IN + DF K +P + PGC V A G C+ P S+ F C C+ G++G C +R + + + + GTC I F Y Sbjct: 26 QACPAQCSCSGSTVDCHGLALRSVPRNIPRNTERLDLNGNNITRITKTDFAGLRHLRVLQLMENKISTIERGAFQDLKELERLRLNRNHLQLFPELLFLGTAKLYRLDLSENQIQAIPRKAFRGAVDIKNLQLDYNQISCIEDGAFRALRDLEVLTLNNNNITRLSVASFNHMPKLRTFRLHSNNLYCDCHLAWLSDWLRQRPRV---GLYTQ-CMGPSHLRGHNVAEVQKREFVCSDEEEGHQSFMAPSCSVLHCPAACTCSNN-IVDCRGKGLTEIPTNLPETITEIRLEQNTIKVIPPGAFSPYKKLRRIDLSNNQISELAPDAFQGLRSLNSLVLYGNKITELPKSLFEGLFSLQLLLLNANKINCLRVDAFQDLHNLNLLSLYDNKLQTIAKGTFSPLRAIQTMHLAQNPFICDCHLKWLADYLHTNPIETSGARCTSPRRLANKRIGQIKSKKFRCSAKEQYFIPGTEDYRSKLSG---DCFADLACPEKCRCEGTTVDCSNQKLNKIPEHIPQYTAELRLNNNEFTVLEATGIFKKLPQLRKINFSNNKITDIEEGAFEGASGVNEILLTSNRLENVQHKMFKGLESLKTLMLRSNRITCVGNDSFIGLSSVRLLSLYDNQITTVAPGAFDTLHSLSTLNLLANPFNCNCYLAWLGEWLRKKRIVTGN---PRCQKPYFLKEIPIQDVAIQDFTCDDGNDD-----NSCSPLSRCPTECTC-----------------LDTVVRCSNKGLKVLPKGIPRDVTELYLDGNQF-TLVPKELSNYKHLTLIDLSNNRISTLSNQSFSNMTQLLTLILSYNRLRCIPPRTFDGLKSLRLLSLHGNDISVVPEGAFNDLSALSHLAIGANPLYCDCNMQWLSDWVKSEYKEPGIARCAGPGEMADKLLLTTPSKKFTCQGPV-DVNILAKCNPCLSNPCKNDGTCNSDPVDFYRCTCPYGFKGQDCDVPIHACISNPCKHGGTCHLKEGEEDG-FWCICADGFEGENCEVNVDDCEDNDCENNSTCVDGINNYTCLCPPEYTAANLNEVEKGELCEEKLDFCAQDLNPCQHDSKCILTPKG-FKCDCTPGYVGEHCDIDFDDCQDNKCKNGAHCTDAVNGYTCICPEGYSGLFCEFSP--PMVLP-----------------------RTSPCDNFDCQNGAQCI----VRINEPICQCLPGYQGEKCEKLVSVNFINKESYLQI-PSAK--VRPQTNITLQIATDEDSGILLYKG-DKDHIAVELYRGRVRASYDTGSHPASAI-YSVETINDGNFHIVELLALDQSLSLSVDGGNPKIITN--LSKQSTLNFDSPLYVGGMPGKSNVASLRQAPGQNGTSFHGCIRNLYINSELQDFQK-VPMQTGILPGCEPCHKKVCA------------------------------------HGTCQ--PSSQAGFTCECQEGWMGPLCDQRTN-------DPCLGNKCVHGTCLPINAFSY 1406 HSP 2 Score: 58.9214 bits (141), Expect = 9.559e-8 Identity = 43/111 (38.74%), Postives = 56/111 (50.45%), Query Frame = 1 Query: 2917 GICKNIDNYGNYECVCPVGFRGPKCTINVDD-CVGNLCVNGATCNDLNN-SYSCKCQSGFRGKYCDIKFLYCNDINPCQ----NSGQCQLLPNNDYKCVCSKGWEGKQCER 3231 G C+ G + C C G+ GP C +D C+GN CV+G TC +N SYSCKC G G CD + + NPCQ G+C+L C CS G+ G C+R Sbjct: 1357 GTCQPSSQAG-FTCECQEGWMGPLCDQRTNDPCLGNKCVHG-TCLPINAFSYSCKCLEGHGGVLCDEEE---DLFNPCQAIKCKHGKCRLSGLGQPYCECSSGYTGDSCDR 1462
BLAST of slit1 vs. Ensembl Celegans
Match: slt-1 (Slit homolog 1 protein [Source:UniProtKB/Swiss-Prot;Acc:G5EFX6]) HSP 1 Score: 627.476 bits (1617), Expect = 0.000e+0 Identity = 452/1534 (29.47%), Postives = 706/1534 (46.02%), Query Frame = 1 Query: 100 LYCFSSTIWINDPSDESEICPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQLQCINDNIFNKLGMLKYLLLEGNKIF---------WISEAISKL-------------KHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCASEDKANLENMCPSDKKVCSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQIYNFQCNKEFESKYETHDEECVPKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLPPIYSIHEAPNLMKLDLSFNQIETLEPDVFTRSSKLREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQFHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCDGNASVYHSVDSCHE-TNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVLSFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCN-DINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNSIYFQK-NSYIEISPPSRGFLMPRGNISLNFMTNSSSGLILYQGSEQTYLVVNLYQGHLRISYNLGDKVVGPEGYSMGMVNDSRLHALHIEIVGENLTMYLNGRVHCTLKSKYLNSNRYMALDTGIFIGGAPHKILQSAIMKFHIETDSEFIGCMESLLINRKKIDFSKYLPKSINVHPGCGKLISTVVASDLKIPSIMXXXXXXXXXXXXXXXNNVCKRPGICKNQGVCEVLPGSKKKFRCICKNGFVGRKCKKRKSMCHGT-FTESHIVDPDSQGTCKSIERFQYRICTGTCSTSRMQSDVIKNFYHKPIDPSSSRCCQPTKYSTHEIQFKCENGRVYRRAANLPKNCKC 4623 L CF + I ES CP C C + +V C + VP + T RL+L N I I +DF L NLK LQL +N+I +I +F +LV L KLRL+RN I++LPD++F KL LDL +N + ++D L+ L L+ N IF W+S + L ++L + N NC+C L W+ +L++ G + +C+TP L I L C+ K + C + ++C C C+ V C L+ +P +LP + E+ L+ N I+ + K L ++ L++N + +I+P AFLGL L ++ L NN+ ++ + F GL SL L L N + C+ + T + +L +L L +N + S+ + TF+ +L L+L NPL CDC + W+ K T A C QP+ L+ L F+C K ES + + C CP +C C V+C+ GL ++P SI L++S NN+ + +IH NL LDLS N I + F + SKLRE+ LN N+L ++ LEIL LS N I C + F ++ + + N CDC++ + W R++ + + +P CQ + D++ D ++C + +N CS + V CS+ L P+ IP DTTELYLD N++ + L +L L LS+N + L N + L L TL++S+NKL+C+ AF L+ L++L L ND+S +P AF++L ++ +IA+G N +CDCN+ W +++ +F++ GI+ C P + + + ++P F C + T KCD C+ PCK+N C + Y C+C F+G +C+ +I+AC+G PC N CK + G + C C GF G C N+DDCV + C NG C DL NSY C C + GK+C+ K YC +NPC+N+G+C + N Y C+CS G+ G CE N DDCK C++ CVD Y C+CRPGY G++CEIP M E + + +S C EC+ QN + + CKC G+SG C S+ F+ +Y+ + P L G I++ T S G++LY G + ++ LY G +++ Y +G+ YS VND H + I + ++ +++ + ++IGG P + Q A +FH++ GC+ S+ IN I+ + L +++N C + N C GI G C S K + C C + F G C +++ C F HI + C+S++R + C G C + CC K +++ C+NG ++ + C+C Sbjct: 2 LICFIFILLI----PESATCPAECVCVDRTVSCVGQQLTEVPQNIPNDTIRLDLQDNEITKIGPNDFSSLMNLKALQLMDNQIVTIHNQSFSSLVFLQKLRLSRNRIRHLPDNVFQNNLKLTHLDLSENDITVVSDAQLQGPEFLEVLNLDKNHIFCLENNVISSWVSLEVLTLNGNRLTTFEEPSNARFRQLDLFNNPWNCDCRLRWMRKWLEKAE-----GQNKTVCATPLNLQGSSIEILQDKFMTCSGNRKRRYKKTCET-AEICPLPCTCT-GTTVDCRDSGLTYVPTNLPPSTTEIRLEQNQISSIPSHSFKNLKNLTRLDLSKNIITEIQPKAFLGLHNLHTLVLYGNNITDLKSDTFEGLGSLQLLLLNANQLTCIRRGTFDHVPKLSMLSLYDNDIKSISEVTFQNLTSLSTLHLAKNPLICDCNLQWLAQINLQKNIETSGARCEQPKRLRKKKFATLPPNKFKC-KGSESFVSMYADSCFIDSICPTQCDCYGTTVDCNKRGLNTIPTSIPRFATQLLLSGNNISTVDLNSNIHVLENLEVLDLSNNHITFINDKSFEKLSKLRELRLNDNKLHHFSSMVLDEQSNLEILDLSGNNIQCFSSIFFNKATRIREIKVIGNDLLCDCRILPLMSWLRSNSS--HSIDIPPCQQ--------FQYSDNES---DKQRCAAFPEETCSDDSNLCPPKCSCLDRV-----------------VRCSNKNLTSFPSRIPFDTTELYLDANYINEIPAHDLNRLYSLTKLDLSHNRLISLENNTFSNLTRLSTLIISYNKLRCLQPLAFNGLNALRILSLHGNDISFLPQSAFSNLTSITHIAVGSNSLYCDCNMAWFSKWIKSKFIEAGIARCEYPNTVSNQLLLTAQPYQFTCDSKVPTKLAT-KCDLCLNSPCKNNAICETTSSRKYTCNCTPGFYGVHCENQIDACYGSPCLNNATCK-VAQAGRFNCYCNKGFEGDYCEKNIDDCVNSKCENGGKCVDLINSYRCDCPMEYEGKHCEDKLEYCTKKLNPCENNGKC-IPINGSYSCMCSPGFTGNNCETNIDDCKNVECQNGGSCVDGILSYDCLCRPGYAGQYCEIPPM------------------MDMEYQKTDACQQSACGQGECV-------ASQNSSDFT-CKCHEGFSGPSCDRQMSVGFKNPGAYLALDP-----LASDGTITMTLRTTSKIGILLYYGDDH-FVSAELYDGRVKLVYYIGN-FPASHMYSSVKVNDGLPHRISIRTSERKCFLQIDKNPVQIVENSGKSDQLITKGKEMLYIGGLPIEKSQDAKRRFHVKNSESLKGCISSITINEVPINLQQAL-ENVNTEQSCSATV------------------------------NFCA--GIDCGNGKCTNNALSPKGYMCQCDSHFSGEHCDEKRIKCDKQKFRRHHI-----ENECRSVDRIKIAECNGYCGGEQ-------------------NCCTAVKKKQRKVKMICKNGTTKISTVHIIRQCQC 1400
BLAST of slit1 vs. Ensembl Celegans
Match: clec-78 (C-type LECtin [Source:UniProtKB/TrEMBL;Acc:Q20531]) HSP 1 Score: 159.844 bits (403), Expect = 8.237e-39 Identity = 100/276 (36.23%), Postives = 137/276 (49.64%), Query Frame = 1 Query: 2773 CMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNN--SYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIP----AWKPTV----LKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYL 3570 C + C++N +CS+ H QC C + G C+ + C QPC NGG C+ + Y+C CP F G +C D+C G C NG C+DL S +C C++GF G C+ C+ NPC+N +C +KC C GWEG C++N DDC + C + CVDL NDYKC C G+ GK C I A P V + L SR+ + Q T EN + + CA C N+ CI ++ C+C GY G CQ+L Sbjct: 2017 CSAKMCENNAECSVFMHR-AQCHCKPGYVGDRCEMLEDVCSTQPCYNGGKCEQVGT--TYKCTCPKMFNGARCQFESDECNGVKCPNGGVCHDLPGVKSTTCLCRTGFAGPQCEEITDICSTNNPCRNGARCIGEKLGRFKCQCVPGWEGPNCDKNIDDCADSPCALNATCVDLINDYKCECPTGFSGKRCHIKENLCASSPCVHGLCIDKLYSRQCLCQPGWTGENCDQNI---DECAASPCQNDAKCI----DEINGYMCECADGYEGVHCQHL 2282 HSP 2 Score: 133.265 bits (334), Expect = 1.226e-30 Identity = 91/277 (32.85%), Postives = 133/277 (48.01%), Query Frame = 1 Query: 2767 DSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKH--ESQCVDLHNDYKCMCRPGYIGKFCEIP----AWKPTV-----LKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQ 3564 D C PC N C + + +Y+C+CP F GK C + N C PC +G +C ID + +C+C G+ G C N+D+C + C N A C D N Y C+C G+ G +C +C PC N+ C + Y C C+ G++G CE N D+C N C C D ND+KC+C+PGY G C++ A P + + + + + +S T E + S CA C NNG C++ + +C C PG SG C+ Sbjct: 2171 DDCADSPCALNATCVDLIN-DYKCECPTGFSGKRCHIKENLCASSPCVHG-LC--IDKLYSRQCLCQPGWTGENCDQNIDECAASPCQNDAKCIDEINGYMCECADGYEGVHCQHLVDHCAK-QPCHNNATCTNM-GATYHCDCTLGFDGVHCEMNIDECAENQCDKLGTESCRDAVNDFKCVCKPGYTGPRCDVKQDQCADSPCLNDAQCVDMGGAYKCVCKSGWTGPKCEQDNGS---CAAKPCRNNGFCVSLVADY----FCVCPPGVSGKNCE 2434 HSP 3 Score: 124.02 bits (310), Expect = 6.399e-28 Identity = 76/243 (31.28%), Postives = 112/243 (46.09%), Query Frame = 1 Query: 2746 AYITAKC----DSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNI----------------------------------------DNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCE 3342 Y+ +C D C +PC + GKC + Y+C CP F+G C + C G C NGG+C ++ + G ++C C G+ GP C N+DDC + C ATC DL N Y C+C +GF GK C IK C +PC + G C + +C+C GW G+ C++N D+C + C+++++C+D N Y C C GY G C+ Sbjct: 2042 GYVGDRCEMLEDVCSTQPCYNGGKCEQV-GTTYKCTCPKMFNGARCQFESDECNGVKCPNGGVCHDLPGVKSTTCLCRTGFAGPQCEEITDICSTNNPCRNGARCIGEKLGRFKCQCVPGWEGPNCDKNIDDCADSPCALNATCVDLINDYKCECPTGFSGKRCHIKENLCAS-SPCVH-GLC-IDKLYSRQCLCQPGWTGENCDQNIDECAASPCQNDAKCIDEINGYMCECADGYEGVHCQ 2280 HSP 4 Score: 121.709 bits (304), Expect = 3.250e-27 Identity = 85/281 (30.25%), Postives = 119/281 (42.35%), Query Frame = 1 Query: 2734 ETEDAYITAKC----DSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQ 3564 E D Y C D C K+PC +N C+ + Y CDC F G +C+ I+ C C G D +++CVC G+ GP+C + D C + C+N A C D+ +Y C C+SG+ G C+ C PC+N+G C L DY CVC G GK CE + C C + +C D + +C C + GK CE +K T KT C N G C + C+C PG++G C+ Sbjct: 2269 ECADGYEGVHCQHLVDHCAKQPCHNNATCTNM-GATYHCDCTLGFDGVHCEMNIDECAENQCDKLGTESCRDAVNDFKCVCKPGYTGPRCDVKQDQCADSPCLNDAQCVDMGGAYKCVCKSGWTGPKCEQDNGSCA-AKPCRNNGFCVSL-VADYFCVCPPGVSGKNCESAPNRCIGQPCHNGGECGDFGSHLECACPASFTGKGCE---FKNTGCKT-------------------------------CENGGKCAEAAGGLQK---CECSPGFTGERCE 2509 HSP 5 Score: 119.013 bits (297), Expect = 2.370e-26 Identity = 79/257 (30.74%), Postives = 114/257 (44.36%), Query Frame = 1 Query: 2587 FHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIY-HSKPTDFICSEETEDAYITAKCD----SCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCE 3342 +HCDC + F + I CA + KL + DF C + Y +CD C PC ++ +C + Y+C C + G C++ +C +PCRN G C ++ +Y CVCP G G C + C+G C NG C D + C C + F GK C+ K C C+N G+C KC CS G+ G++CE N D+C C + CVD N + C+C G C+ Sbjct: 2304 YHCDCTLG-----FDGVHCEMNIDECAENQCDKLGTESCRDAVNDFKCV--CKPGYTGPRCDVKQDQCADSPCLNDAQC-VDMGGAYKCVCKSGWTGPKCEQDNGSCAAKPCRNNGFCVSL--VADYFCVCPPGVSGKNCESAPNRCIGQPCHNGGECGDFGSHLECACPASFTGKGCEFKNTGCKT---CENGGKCAEAAGGLQKCECSPGFTGERCETNIDECSTAHCPSGATCVDQVNTHICVCPFNLTGVHCD 2547
BLAST of slit1 vs. Ensembl Celegans
Match: Y46G5A.29 (pep chromosome:WBcel235:II:12856200:12862058:1 gene:WBGene00012917.1 transcript:Y46G5A.29.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:Y46G5A.29) HSP 1 Score: 112.849 bits (281), Expect = 7.483e-25 Identity = 67/156 (42.95%), Postives = 91/156 (58.33%), Query Frame = 1 Query: 2890 CFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATC-NDLNN--SYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIP 3348 C PC+NGG C + G C C G+ G CT +D C+ N C N +TC ND + YSCKCQ+G GK C+I+ C + C+N GQC L C C G G++CER ++C +NSCK+ ++CVDL NDY+C C G++G+ CE P Sbjct: 370 CQDNPCQNGGRCSM--SKGKSTCQCNPGYVGANCT-EIDKCLDNECDNNSTCFNDKRSPVGYSCKCQNGTVGKLCEIE---CPK-DQCENGGQCYLNTFGKIGCKCVPGTTGRRCEREINECGWNSCKNGAKCVDLFNDYRCDCADGWMGRNCERP 518 HSP 2 Score: 80.1073 bits (196), Expect = 9.165e-15 Identity = 77/277 (27.80%), Postives = 116/277 (41.88%), Query Frame = 1 Query: 2479 HNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYG-NYECVCPVGFRGPKCTINVDDCVGNLCVNGATCN-DLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSC---KHESQCVDLHN 3294 H+ K LI + ++ + + + F LN+ L ++ ++K+LN R L + DF+ + + E + C PC++ G+CS+ + C C + G NC + I+ C C N C N Y C C G G C I +C + C NG C + CKC G G+ C+ + C N C+N +C L NDY+C C+ GW G+ CER D Y SC K E QC ++ N Sbjct: 306 HSSKFLIEKPSESTFMMGRMFKKFAPLNDTVLQED------DLKFLNSHVHHRMLQH----------------------DFVDNNDLEME------EKCQDNPCQNGGRCSM-SKGKSTCQCNPGYVGANCTE-IDKCLDNECDNNSTCFNDKRSPVGYSCKCQNGTVGKLCEI---ECPKDQCENGGQCYLNTFGKIGCKCVPGTTGRRCEREINECG-WNSCKNGAKCVDL-FNDYRCDCADGWMGRNCERPCQDI-YGSCRVWKREGQCQEMRN 540
BLAST of slit1 vs. Ensembl Celegans
Match: glp-1 (pep chromosome:WBcel235:III:9092241:9099698:1 gene:WBGene00001609.1 transcript:F02A9.6.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:glp-1) HSP 1 Score: 106.686 bits (265), Expect = 1.098e-22 Identity = 83/299 (27.76%), Postives = 130/299 (43.48%), Query Frame = 1 Query: 2722 ICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDC-VGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCE-----------------RNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQ 3564 IC+ +Y D C + C C + + +NY CDCP G+ C++ A G C +G N D N+ CVC G+ G C + ++C + CVN +TC +L+ ++C C+ G+ GKYC+ C D CQN G C N C C +G+ G++CE R +C ++ +C++ C+C P YIG CE IN+ + + ++++ C Y+ C+NN CI+ KN C C G+ G C+ Sbjct: 141 ICNNGYGGSYCEEGIDHCAQNECAEGSTC-VNSVYNYYCDCPIGKSGRYCERTECALMGNICNHGRCIPNRDEDKNFRCVCDSGYEGEFCNKDKNECLIEETCVNNSTCFNLHGDFTCTCKPGYAGKYCEEAIDMCKDY-VCQNDGYCAHDSNQMPICYCEQGFTGQRCEIECPSGFGGIHCDLPLQRPHCSRSNGTCYNDGRCINGF----CVCEPDYIGDRCE-----------------INRKDFKFPDIQS-------CKYNPCVNNATCID---LKNSGYSCHCPLGFYGLNCE 406 HSP 2 Score: 105.531 bits (262), Expect = 2.125e-22 Identity = 77/248 (31.05%), Postives = 115/248 (46.37%), Query Frame = 1 Query: 2839 DCPYP-FHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNND----YKCVCSKGWEGKQCERNTDDCKY-NSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQ 3564 DC P F G +N C PC NG +C YG ++C+C G+ G C +D C N C G+TC + +Y C C G G+YC+ C + N G+C +PN D ++CVC G+EG+ C ++ ++C +C + S C +LH D+ C C+PGY GK+CE E I +C + C N+G C + + NQ C C+ G++G C+ Sbjct: 109 DCKTPLFSG------VNPCDSDPCNNG-LCYPF--YGGFQCICNNGYGGSYCEEGIDHCAQNECAEGSTCVNSVYNYYCDCPIGKSGRYCE--RTECALMGNICNHGRC--IPNRDEDKNFRCVCDSGYEGEFCNKDKNECLIEETCVNNSTCFNLHGDFTCTCKPGYAGKYCE------------------------------EAI--DMCKDYVCQNDGYCAH---DSNQMPICYCEQGFTGQRCE 308 HSP 3 Score: 89.3521 bits (220), Expect = 1.844e-17 Identity = 76/267 (28.46%), Postives = 115/267 (43.07%), Query Frame = 1 Query: 2773 CMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATC--NDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYK-CMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQ 3564 C PC +NG C + +QC C + G C++ I+ C C G C +++ NY C CP+G G C +GN+C +G D + ++ C C SG+ G++C+ C C N+ C L + D+ C C G+ GK CE D CK C+++ C N C C G+ G+ CEI P+ + + + + N C N+G CING +C C+P Y G C+ Sbjct: 121 CDSDPC-NNGLCYPF-YGGFQCICNNGYGGSYCEEGIDHCAQNECAEGSTC--VNSVYNYYCDCPIGKSGRYCERTECALMGNICNHGRCIPNRDEDKNFRCVCDSGYEGEFCNKDKNECLIEETCVNNSTCFNL-HGDFTCTCKPGYAGKYCEEAIDMCKDYVCQNDGYCAHDSNQMPICYCEQGFTGQRCEIEC--PSGFGGIHCDLPLQRPHCSRSN-------------GTCYNDGRCING--------FCVCEPDYIGDRCE 359 HSP 4 Score: 83.9593 bits (206), Expect = 9.014e-16 Identity = 93/301 (30.90%), Postives = 129/301 (42.86%), Query Frame = 1 Query: 2716 DFICSEETEDA--YITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFG---------QP--CRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDD--------CVGNLCVNGATCNDLNNS-YSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCE-RNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCE----IPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCK 3537 DF C+ + A Y D C C+++G C+ ++ C C F G+ C+ + FG +P R+ G C N N CVC + G +C IN D C N CVN ATC DL NS YSC C GF G C+ + L C C N G C+ + N +C C G+ G CE ++ C + CK+ C N C C+ GY G CE I K TV++ L +R +L + I C EC +G GD + Q+ + KC+ Sbjct: 254 DFTCTCKPGYAGKYCEEAIDMCKDYVCQNDGYCAHDSNQMPICYCEQGFTGQRCEIECPSGFGGIHCDLPLQRPHCSRSNGTCYNDGRCINGFCVCEPDYIGDRCEINRKDFKFPDIQSCKYNPCVNNATCIDLKNSGYSCHCPLGFYGLNCE-QHLLCTPTT-CANGGTCEGV-NGVIRCNCPNGFSGDYCEIKDRQLCSRHPCKNGGVC---KNTGYCECQYGYTGPTCEEVLVIEKSKETVIRDLCEQRKC-------MDLASNGICNPECNLEECNFDG----GDCSGGQRPFSKCQ 537 HSP 5 Score: 76.2554 bits (186), Expect = 2.014e-13 Identity = 81/316 (25.63%), Postives = 122/316 (38.61%), Query Frame = 1 Query: 2722 ICSEETEDAYITAKCDSCM-KRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNL------------CVNGATCNDLN--NSYSCKCQSGFRGKYCDI--KFLYCNDI-----NPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNSIYFQKNSYI 3603 +C E + + C+ + C +N C H ++ C C + GK C++ I+ C C+N G C + N C C GF G +C I G + NG ND N + C C+ + G C+I K DI NPC N+ C L N+ Y C C G+ G CE++ C +C + C ++ +C C G+ G +CEI +C+ H C N G+C N YC+C+ GY+G C+ + I K + I Sbjct: 219 VCDSGYEGEFCNKDKNECLIEETCVNNSTC-FNLHGDFTCTCKPGYAGKYCEEAIDMCKDYVCQNDGYCAHDSNQMPI-CYCEQGFTGQRCEIECPSGFGGIHCDLPLQRPHCSRSNGTCYNDGRCINGF-CVCEPDYIGDRCEINRKDFKFPDIQSCKYNPCVNNATCIDLKNSGYSCHCPLGFYGLNCEQHLL-CTPTTCANGGTCEGVNGVIRCNCPNGFSGDYCEIK-------------------------------DRQLCSRHPCKNGGVCKN-------TGYCECQYGYTGPTCEEVLVIEKSKETVI 492 HSP 6 Score: 59.6918 bits (143), Expect = 2.227e-8 Identity = 71/257 (27.63%), Postives = 100/257 (38.91%), Query Frame = 1 Query: 2905 CRNGGICKNIDNYGNYECVCPVGFRGPKCTINVD-DC-VGNLCVNGA-------TCNDLNNSYSCK------CQSGFRGKYCDIKFLYCNDINPCQ----NSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTL------ISRRDINQS-----EMTYENL-----ENEVISESVCAYHECLNNGICIN--GDQNKNQKSYCKCKPGYSGHLCQ 3564 C G C N Y C C GF G C D DC G C+ C D + C+ G+ G C + +NPC N+G C ++C+C+ G+ G CE D C N C S CV+ +Y C C G G++CE + ++ + I RD +++ + YE +NE + E C+NN C N GD C CKPGY+G C+ Sbjct: 29 CSVNGKCYNGKLIETYWCRCKKGFGGAFCERECDLDCKRGEKCIYDVYGENPTCICQDCEDETPPTERTQKGCEEGYGGPDCKTPLF--SGVNPCDSDPCNNGLCYPF-YGGFQCICNNGYGGSYCEEGIDHCAQNECAEGSTCVNSVYNYYCDCPIGKSGRYCE--RTECALMGNICNHGRCIPNRDEDKNFRCVCDSGYEGEFCNKDKNECLIEET-----CVNNSTCFNLHGDFT------CTCKPGYAGKYCE 269
BLAST of slit1 vs. Ensembl Celegans
Match: crb-1 (Drosophila CRumBs homolog [Source:UniProtKB/TrEMBL;Acc:Q19350]) HSP 1 Score: 104.76 bits (260), Expect = 4.185e-22 Identity = 83/287 (28.92%), Postives = 126/287 (43.90%), Query Frame = 1 Query: 2767 DSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCR-PGYIGKFCE--------------------IPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSY-CKCKPGYSGHLC 3561 + C C G CS + +N C+C + G +C +++ C PC N G+C+ D + C C F G +C I C+ CV+G +++C C G+ G CD K YC PC N C+ YKC C+ G+EG CE N D+C CK+ ++C D NDY+C+C G+ G+ C + +K I R ++ T + N IS C + +C+N + I ++N+ Y C C GY+G C Sbjct: 1369 NQCSSNTCSSRGACSPV-WNNTVCNCDNNWRGAHCQHQMDTCLDFPCNNDGVCRTNDE-NTFSCECQKFFMGTRCEIE-GSCLKAQCVHGECIQLSPETHTCSCNIGYEGDACDKKIDYCK-AGPCLNGANCENKLTG-YKCTCAVGFEGADCEINIDECALEFCKNGAKCRDKINDYECVCDGTGFEGRNCTTDINECANPNNCINGECTNTLGNYKCACRNGFIGPRCSVRNPCTAQIASNN-ISSVTCVHGKCVNPVVQI--EKNREVAKYECACDRGYTGPTC 1647 HSP 2 Score: 104.375 bits (259), Expect = 5.212e-22 Identity = 74/222 (33.33%), Postives = 105/222 (47.30%), Query Frame = 1 Query: 2734 ETEDAYITAKCD---SCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQ-SGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCE-RN------------TDDCKYNSCKHESQCVDLHND---YKCMCRPGYIGKFC 3339 E + ++ +C+ SC+K C H G+C ++ + C C + G CDK+I+ C PC NG C+N Y+C C VGF G C IN+D+C C NGA C D N Y C C +GF G+ C C + N C N G+C N YKC C G+ G +C RN + C + C + ++ + + Y+C C GY G C Sbjct: 1431 ECQKFFMGTRCEIEGSCLKAQCVH-GECIQLSPETHTCSCNIGYEGDACDKKIDYCKAGPCLNGANCEN--KLTGYKCTCAVGFEGADCEINIDECALEFCKNGAKCRDKINDYECVCDGTGFEGRNCTTDINECANPNNCIN-GECTNTLGN-YKCACRNGFIGPRCSVRNPCTAQIASNNISSVTCVHGKCVNPVVQIEKNREVAKYECACDRGYTGPTC 1647 HSP 3 Score: 103.605 bits (257), Expect = 1.059e-21 Identity = 127/485 (26.19%), Postives = 201/485 (41.44%), Query Frame = 1 Query: 2806 CSLI--THHNYQCDCPYPFHGKNCDK-RINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCV-GNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLY-----CNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTL-------ISRRD-INQSEMTYENLEN-----EVISESVCAYHE------------------CLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNSIYFQKNSYIEISPPSRGFLMPRGNISLNFMTNSSSGLILYQGSEQTYLVVNLYQGHLRISYN-LGDKVVGPE---GY-SMGMVNDSRLHALHIEIV-GENLTMYLNGRVHCTLKSKYLNSNRYMALDTGIFIGGAPHKILQSAIMKF-HIETDSEFIGCMESLLINRKKIDFSKYLPKSINVHPGCGK 4119 CSL+ T QC+CP F G +C + + C +PC NGG C + D + C C F G C VD V G+ C NG C + +C+C GF G C+I Y C+DI C N G C+L + CVC +G++G CE + C N C++ C D Y C C G+ G CE T + TL S D +QS+++ E+ N +V S VC + C + C + N N + C C+ G+ G C+ + S F S ++ SL SS I Y+ + + HL S N LG+K++ + GY M +S H L + + + T+++ G + ++ + N + + + + L+ + +F I FIGCM + ++ I F+ ++I++ GC + Sbjct: 255 CSLVNGTSRTTQCECPSGFMGADCKELQARPCDREPCLNGGHCVD-DGQNLFTCFCLPSFTGIYCGEPVDCLVNGSDCKNGGKCVFALAATTCQCPEGFNGSNCEISNSYRSHPTCSDIR-CLNGGSCKLDAEGEPFCVCEEGFDGPFCEPKSG-CTINPCQNGGTCQDADGQYFCHCTSGFGGVHCETVDEPSTPIPTLGTFPSFTTSGIDGFSQSKISCEDCVNSSNCLDVESGPVCICDDGYFGQKCDQKHDKCSKVSCPSGQTCSQVNDNVNITAQCGCEIGHFGQQCEMVTSATFSAKSL---------YIHQSSKFSLG---TSSFENIAYE--LEFSFRTTVENTHLASSENILGEKILSIQLLSGYLVFNMTGNSLEHLLPMRVSDAQWYTVFVKGEDN-KIQIEVSTENGFSLVQKSV------NGQLEVFLTRFGKISGTHHFIGCMADVRVDGDLIIFADN-KRAIDIRKGCTR 714 HSP 4 Score: 96.2857 bits (238), Expect = 1.634e-19 Identity = 83/299 (27.76%), Postives = 128/299 (42.81%), Query Frame = 1 Query: 2797 NGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATC-NDLNNSYSCKCQSGFRGKYCDI-KFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCE-RNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCE-----------------IPAWKPTVLKTLISRRDIN-QSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNSIYFQK---NSYIEISPPS 3621 NG + + +C CP F G+NC+ +N C C G C +D +C+C GF G C + D+C G C NG C + + C+C + + G C++ K C D +PCQN GQC + ++C C G+ G+ CE R+ ++C + C + CV + C C GY G+FCE P W TV + R + Q +M C C N+G+C D+N C+C+ + G C+ S + I++SP + Sbjct: 1185 NGATCVDIWNKRKCVCPAGFAGENCEDNVNDCKFVDCGKHGYC--LDGIDEAKCICNNGFHGEHCELAKDECEGVECHNGGKCVKNRSEKIVCQCGNSWMGDSCNVTKTTNCKD-SPCQNFGQCMQKTDTFFECNCMDGYSGELCEQRDVNECNHYDC-NRGHCVMTVSGPACQCEMGYTGRFCEKLLNQCSSNTCSSRGACSPVWNNTVCNCDNNWRGAHCQHQM------------DTCLDFPCNNDGVCRTNDENTFS---CECQKFFMGTRCEIEGSCLKAQCVHGECIQLSPET 1464 HSP 5 Score: 92.0485 bits (227), Expect = 3.350e-18 Identity = 71/234 (30.34%), Postives = 99/234 (42.31%), Query Frame = 1 Query: 2869 CDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCE--RNTDDCKYNSCKHESQCVD-LHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLC 3561 C +C C+N G C + GN +C CP GF G C + D+C N C NGA C +L SY CKC GF GKYC+I+ + C N+GQC + +D C CS G++G CE ++C + H V+ +C C G++G C+ +P C CLN G C++ QN C C P ++G C Sbjct: 122 CQSNEPSCATHTCQNNGTC--VAENGNVKCACPPGFVGDHCETDEDECKENFCQNGADCENLKGSYECKCLKGFSGKYCEIQDKKQCTSDYCHNNGQC-ISTGSDLSCKCSPGFDGAFCELKAEVNECICENPAHVCSLVNGTSRTTQCECPSGFMGADCKELQARP-------------------------------CDREPCLNGGHCVDDGQN---LFTCFCLPSFTGIYC 318 HSP 6 Score: 88.1965 bits (217), Expect = 4.244e-17 Identity = 86/296 (29.05%), Postives = 125/296 (42.23%), Query Frame = 1 Query: 2743 DAYITAKCDSCMKRPCKH----NGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATC--NDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNS----GQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTV--LKTLISRRD-INQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSY-CKCKPGYSGHLCQYLNSIYFQ 3588 D Y C+ C H G C ++T C C + G+ C+K +N C C + G C + + N C C +RG C +D C+ C N C ND N++SC+CQ F G C+I+ C + G+C L + C C+ G+EG C++ D CK C + + C + YKC C G+ G CEI + + K RD IN E + E + + +EC N CING+ +Y C C+ G+ G C N Q Sbjct: 1319 DGYSGELCEQRDVNECNHYDCNRGHC-VMTVSGPACQCEMGYTGRFCEKLLNQCSSNTCSSRGACSPV--WNNTVCNCDNNWRGAHCQHQMDTCLDFPCNNDGVCRTND-ENTFSCECQKFFMGTRCEIE-------GSCLKAQCVHGECIQLSPETHTCSCNIGYEGDACDKKIDYCKAGPCLNGANCENKLTGYKCTCAVGFEGADCEINIDECALEFCKNGAKCRDKINDYECVCDGTGFEGRNCTT-DINECANPNNCINGECTNTLGNYKCACRNGFIGPRCSVRNPCTAQ 1602 HSP 7 Score: 82.0333 bits (201), Expect = 3.556e-15 Identity = 75/255 (29.41%), Postives = 105/255 (41.18%), Query Frame = 1 Query: 2788 CKHNGKCSLITHHNYQCDCPYPFHGKNCD-----KRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDI---------------------------KFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDY----KCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMT--YENLENEV 3438 CK+ GKC + C CP F+G NC+ + C C NGG CK +D G CVC GF GP C C N C NG TC D + Y C C SGF G +C+ + C D C NS C L + C+C G+ G++C++ D C SC C ++++ +C C G+ G+ CE+ K+L + S T +EN+ E+ Sbjct: 331 CKNGGKC-VFALAATTCQCPEGFNGSNCEISNSYRSHPTCSDIRCLNGGSCK-LDAEGEPFCVCEEGFDGPFCEPK-SGCTINPCQNGGTCQDADGQYFCHCTSGFGGVHCETVDEPSTPIPTLGTFPSFTTSGIDGFSQSKISCED---CVNSSNC-LDVESGPVCICDDGYFGQKCDQKHDKCSKVSCPSGQTCSQVNDNVNITAQCGCEIGHFGQQCEMVTSATFSAKSLYIHQSSKFSLGTSSFENIAYEL 578 HSP 8 Score: 81.6481 bits (200), Expect = 4.354e-15 Identity = 64/224 (28.57%), Postives = 97/224 (43.30%), Query Frame = 1 Query: 2905 CRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCE-RNTDDCKYNSCKHESQCVDLHND-YKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYL 3570 C NG C +D + +CVCP GF G C NV+DC C C D + C C +GF G++C++ C + C N G+C + C C W G C T +CK + C++ QC+ + ++C C GY G+ CE +RD+N+ C +++C N G C+ C+C+ GY+G C+ L Sbjct: 1183 CLNGATC--VDIWNKRKCVCPAGFAGENCEDNVNDCKFVDCGKHGYCLDGIDEAKCICNNGFHGEHCELAKDECEGVE-CHNGGKCVKNRSEKIVCQCGNSWMGDSCNVTKTTNCKDSPCQNFGQCMQKTDTFFECNCMDGYSGELCE--------------QRDVNE-----------------CNHYDC-NRGHCV----MTVSGPACQCEMGYTGRFCEKL 1367 HSP 9 Score: 63.5438 bits (153), Expect = 1.560e-9 Identity = 64/257 (24.90%), Postives = 103/257 (40.08%), Query Frame = 1 Query: 2833 QCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTIN-VDDCVGNLCVNGATCNDLNNS-YSCKCQSGFRGKYCDIKFLYCNDINPCQ----NSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCE--------IPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLC 3561 +C C FHG++C+ + C G C NGG C + C C + G C + +C + C N C ++ + C C G+ G+ C+ + D+N C N G C ++ + C C G+ G+ CE+ + C N+C C + N+ C C + G C+ P V +T + + + E+ E C +C+ +G CI Q + C C GY G C Sbjct: 1235 KCICNNGFHGEHCELAKDECEGVECHNGGKCVK-NRSEKIVCQCGNSWMGDSCNVTKTTNCKDSPCQNFGQCMQKTDTFFECNCMDGYSGELCEQR-----DVNECNHYDCNRGHC-VMTVSGPACQCEMGYTGRFCEKLLNQCSSNTCSSRGACSPVWNNTVCNCDNNWRGAHCQHQMDTCLDFPCNNDGVCRTNDENTFSCECQKFFMGTRCEI--EGSCLKAQCV-HGECI---QLSPETHTCSCNIGYEGDAC 1478
BLAST of slit1 vs. Ensembl Fly
Match: sli (gene:FBgn0264089 transcript:FBtr0330730) HSP 1 Score: 738.413 bits (1905), Expect = 0.000e+0 Identity = 485/1503 (32.27%), Postives = 747/1503 (49.70%), Query Frame = 1 Query: 157 CPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQLQCINDNIFNKLGMLKYLLLEGNKIFWISEAI-SKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCAS-EDKANLENMCPSDKKVCSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQIYNFQCN-KEFESKYETHDEECVPKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLPPIYSIHEAPNLMKLDLSFNQIETLEPDVFTRSSKLREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQFHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCDGNASVYHSVDSCHETNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVLSFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNL-CVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCN-DINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNSIYF-QKNSYIEISPPSRGFLMPRGNISLNFMTNSSSGLILYQGSEQTYLVVNLYQGHLRISYNLGDKVVGPEGYSMGMVNDSRLHALHIEIVGENLTMYLNGRVHCTLKSKYLN--SNRYMALDTGIFIGGAPHKILQSAIMKFHIETDSEFIGCMESLLINRKKIDFSKYLPKSINVHPGCGKLISTVVASDLKIPSIMXXXXXXXXXXXXXXXNNVCKRPGICKNQGVCEVLPGSKKK--FRCICKNGFVGRKCKKRKSMCHGTFTESHIVDPDSQGTCKSIERFQYRICTGTCSTSRMQSDVIKNFYHKPIDPSSSRCCQPTKYSTHEIQFKCENGRVYRRAANLPKNCKCVANC 4635 CP C C +V+C HR +VP + RL L GN + VI ++DF +L L++LQL++N+I +IE +F +LV+L +L ++ N+I + +F L+ L L +NQ+ C++++ F L L+ L L N + + I L L+ LR+ N C+CHL+WL+ FL+ + + A C +P +L ++ L EF+C+ + A +E C ++ C C C+ DGIV C K L+++P LP + EL L+ N IT L S ++ L++I L+ N + I A GL QLT++ L N +K + F GL SL L L N+I C+ KD L LL L +N + SL TF +++ ++L NP CDC + W+ DY+ T A C P+ + + +L+ F+C+ E K EC CP C C V+C+ GL +P I T L+++ N L ++ P+L+KL+L NQ+ +EP+ F +S ++E+ L N++K I+N+ F L +L+ L L +N ISC++ SF++ +L L L N F+C+C L W +EW R G R C +P ++D I L F C S + C C+ T V CS QLKEIP IP +T+ELYL+ N ++ ++ ++L +L L LS N IT L+ L L TL++S+NKLQC+ + A L+NL+VL L N +S +P +F DLK+L +IALG NP +CDC +KW + + +++ GI+ CA PE+MK K I + + F+C + I AKC++C ++PC++ +C + YQC C +HGK+C+ I+AC+G PCRN C ++ G + C C G+ G +C N+DDC+G + C N ATC D SY C+CQ GF G++CD K +C+ + NPC N +C + Y C C G+ G C N DDC+ + C++ CVD NDY+C C Y GK+CE N M Y S C HEC +G+C + + C+C PGY+G C+YL SI F NS++E+ P P N+++ F + +G+++Y G + +L V L+ G +R+SY++G+ V YS MV D + HA+ + + +N T+ RV L +N SN Y+ L T +F+GG P Q A + I + F GCM+ + IN K +DF + + PGC L + M I + N C+R C +P S + ++C CK+G GR C + S C + + D C+S + +Y C G C ++CC +++ C N R Y + ++ + C C C Sbjct: 73 CPRVCSCTGLNVDCSHRGLTSVPRKISADVERLELQGNNLTVIYETDFQRLTKLRMLQLTDNQIHTIERNSFQDLVSLERLDISNNVITTVGRRVFKGAQSLRSLQLDNNQITCLDEHAFKGLVELEILTLNNNNLTSLPHNIFGGLGRLRALRLSDNPFACDCHLSWLSRFLRSATRL----APYTRCQSPSQLKGQNVADLHDQEFKCSGLTEHAPME--CGAENS-CPHPCRCA-DGIVDCREKSLTSVPVTLPDDTTELRLEQNFITELPPKSFSSFRRLRRIDLSNNNISRIAHDALSGLKQLTTLVLYGNKIKDLPSGVFKGLGSLQLLLLNANEISCIRKDAFRDLHSLSLLSLYDNNIQSLANGTFDAMKSIKTVHLAKNPFICDCNLRWLADYLHKNPIETSGARCESPKRMHRRRIESLREEKFKCSWDELRMKLSG---ECRMDSDCPAMCHCEGTTVDCTGRGLKEIPRDIPLHTTELLLNDNELGRISSDGLFGRLPHLVKLELKRNQLTGIEPNAFEGASHIQELQLGENKIKEISNKMFLGLHQLKTLNLYDNQISCVMPGSFEHLNSLTSLNLASNPFNCNCHLAWFAEWLRKKSLNGGAAR---CGAPSKVRDVQIKDLPHSEFKCSSENS-----EGCLGDGYCPPSCTC-----------------TGTVVRCSRNQLKEIPRGIPAETSELYLESNEIEQIHYERIRHLRSLTRLDLSNNQITILSNYTFANLTKLSTLIISYNKLQCLQRHALSGLNNLRVLSLHGNRISMLPEGSFEDLKSLTHIALGSNPLYCDCGLKWFSDWIKLDYVEPGIARCAEPEQMKDKLILSTPSSSFVCRGRVRND-ILAKCNACFEQPCQNQAQCVALPQREYQCLCQPGYHGKHCEFMIDACYGNPCRNNATCTVLEE-GRFSCQCAPGYTGARCETNIDDCLGEIKCQNNATCIDGVESYKCECQPGFSGEFCDTKIQFCSPEFNPCANGAKC-MDHFTHYSCDCQAGFHGTNCTDNIDDCQNHMCQNGGTCVDGINDYQCRCPDDYTGKYCE----------------GHNMISMMYPQT-------SPCQNHEC-KHGVCFQPNA-QGSDYLCRCHPGYTGKWCEYLTSISFVHNNSFVELEPLR---TRPEANVTIVFSSAEQNGILMYDG-QDAHLAVELFNGRIRVSYDVGNHPVSTM-YSFEMVADGKYHAVELLAIKKNFTL----RVDRGLARSIINEGSNDYLKLTTPMFLGGLPVDPAQQAYKNWQIRNLTSFKGCMKEVWINHKLVDFGN-AQRQQKITPGCALLEGEQQEEEDDEQDFMDETPHIKEEPVDPCLENKCRRGSRC--------VPNSNARDGYQCKCKHGQRGRYCDQAASTCRKEQVREYYTEND----CRSRQPLKYAKCVGGC---------------------GNQCCAAKIVRRRKVRMVCSNNRKYIKNLDIVRKCGCTKKC 1468 HSP 2 Score: 181.415 bits (459), Expect = 2.002e-45 Identity = 135/468 (28.85%), Postives = 219/468 (46.79%), Query Frame = 1 Query: 1504 VPKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNL--------KKLPPIY---------------SIHEAPNLMKLDLSFNQIETLEPDVFTRSSKLREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQFHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCDGNASVYHSVDSCHETNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVLSFNKLQ------------------------CIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKC 2766 + + CP C+C+ V+CS+ GL S+P I + L + NNL ++L + S + +L +LD+S N I T+ VF + LR + L++N++ C++ FK L ELEIL L+ N ++ + + F L+ L L+ N F CDC L W+S + R+ + R CQSP LK + L D+ F C G H+ C NS C + VDC + L +P +P DTTEL L++N + L SF + L+ + LS N I+ + + L+ LK L TLVL NK++ CI +DAF++LH+L +L L N++ ++ F+ +K++ + L +NPF CDCN++WL + ++ + C SP++M + I + F CS + ++ +C Sbjct: 68 ITEARCPRVCSCTGLNVDCSHRGLTSVPRKISADVERLELQGNNLTVIYETDFQRLTKLRMLQLTDNQIHTIERNSFQDLVSLERLDISNNVITTVGRRVFKGAQSLRSLQLDNNQITCLDEHAFKGLVELEILTLNNNNLTSLPHNIFGGLGRLRALRLSDNPFACDCHLSWLSRFLRSATRLAPYTR---CQSPSQLKGQNVADLHDQEFKCSGLTE--HAPMECGAENSCPHPCRCAD-----------------GIVDCREKSLTSVPVTLPDDTTELRLEQNFITELPPKSFSSFRRLRRIDLSNNNISRIAHDALSGLKQLTTLVLYGNKIKDLPSGVFKGLGSLQLLLLNANEISCIRKDAFRDLHSLSLLSLYDNNIQSLANGTFDAMKSIKTVHLAKNPFICDCNLRWLADYLHKNPIETSGARCESPKRMHRRRIESLREEKFKCSWDELRMKLSGEC 513
BLAST of slit1 vs. Ensembl Fly
Match: sli (gene:FBgn0264089 transcript:FBtr0330729) HSP 1 Score: 731.865 bits (1888), Expect = 0.000e+0 Identity = 483/1510 (31.99%), Postives = 749/1510 (49.60%), Query Frame = 1 Query: 157 CPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQLQCINDNIFNKLGMLKYLLLEGNKIFWISEAI-SKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCAS-EDKANLENMCPSDKKVCSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQIYNFQCN-KEFESKYETHDEECVPKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLPPIYSIHEAPNLMKLDLSFNQIETLEPDVFTRSSKLREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQFHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCDGNASVYHSVDSCHETNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVLSFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNL-CVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCN-DINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNSIYF-QKNSYIEISPPSRGFLMPRGNISLNFMTNSSSGLILYQGSEQTYLVVNLYQGHLRISYNLGDKVVGPEGYSMGMVNDSRLHALHIEIVGENLTMYLNGRVHCTLKSKYLN--SNRYMALDTGIFIGGAPHKILQSAIMKFHIETDSEFIGCMESLLINRKKIDFSKYLPKSINVHPGCGKLISTVVASDLKIPSIMXXXXXXXXXXXXXXXNNVCKRPGICKNQGVCEVLPGSKKK--FRCICKNGFVGRKCKKRKS-------MCHGTFTESHIVDPDSQGTCKSIERFQYRICTGTCSTSRMQSDVIKNFYHKPIDPSSSRCCQPTKYSTHEIQFKCENGRVYRRAANLPKNCKCVANC 4635 CP C C +V+C HR +VP + RL L GN + VI ++DF +L L++LQL++N+I +IE +F +LV+L +L ++ N+I + +F L+ L L +NQ+ C++++ F L L+ L L N + + I L L+ LR+ N C+CHL+WL+ FL+ + + A C +P +L ++ L EF+C+ + A +E C ++ C C C+ DGIV C K L+++P LP + EL L+ N IT L S ++ L++I L+ N + I A GL QLT++ L N +K + F GL SL L L N+I C+ KD L LL L +N + SL TF +++ ++L NP CDC + W+ DY+ T A C P+ + + +L+ F+C+ E K EC CP C C V+C+ GL +P I T L+++ N L ++ P+L+KL+L NQ+ +EP+ F +S ++E+ L N++K I+N+ F L +L+ L L +N ISC++ SF++ +L L L N F+C+C L W +EW R G R C +P ++D I L F C S + C C+ T V CS QLKEIP IP +T+ELYL+ N ++ ++ ++L +L L LS N IT L+ L L TL++S+NKLQC+ + A L+NL+VL L N +S +P +F DLK+L +IALG NP +CDC +KW + + +++ GI+ CA PE+MK K I + + F+C + I AKC++C ++PC++ +C + YQC C +HGK+C+ I+AC+G PCRN C ++ G + C C G+ G +C N+DDC+G + C N ATC D SY C+CQ GF G++CD K +C+ + NPC N +C + Y C C G+ G C N DDC+ + C++ CVD NDY+C C Y GK+CE N M Y S C HEC +G+C + + C+C PGY+G C+YL SI F NS++E+ P P N+++ F + +G+++Y G + +L V L+ G +R+SY++G+ V YS MV D + HA+ + + +N T+ RV L +N SN Y+ L T +F+GG P Q A + I + F GCM+ + IN K +DF + + PGC L + M I + N C+R C +P S + ++C CK+G GR C + + T + + + ++ C+S + +Y C G C ++CC +++ C N R Y + ++ + C C C Sbjct: 73 CPRVCSCTGLNVDCSHRGLTSVPRKISADVERLELQGNNLTVIYETDFQRLTKLRMLQLTDNQIHTIERNSFQDLVSLERLDISNNVITTVGRRVFKGAQSLRSLQLDNNQITCLDEHAFKGLVELEILTLNNNNLTSLPHNIFGGLGRLRALRLSDNPFACDCHLSWLSRFLRSATRL----APYTRCQSPSQLKGQNVADLHDQEFKCSGLTEHAPME--CGAENS-CPHPCRCA-DGIVDCREKSLTSVPVTLPDDTTELRLEQNFITELPPKSFSSFRRLRRIDLSNNNISRIAHDALSGLKQLTTLVLYGNKIKDLPSGVFKGLGSLQLLLLNANEISCIRKDAFRDLHSLSLLSLYDNNIQSLANGTFDAMKSIKTVHLAKNPFICDCNLRWLADYLHKNPIETSGARCESPKRMHRRRIESLREEKFKCSWDELRMKLSG---ECRMDSDCPAMCHCEGTTVDCTGRGLKEIPRDIPLHTTELLLNDNELGRISSDGLFGRLPHLVKLELKRNQLTGIEPNAFEGASHIQELQLGENKIKEISNKMFLGLHQLKTLNLYDNQISCVMPGSFEHLNSLTSLNLASNPFNCNCHLAWFAEWLRKKSLNGGAAR---CGAPSKVRDVQIKDLPHSEFKCSSENS-----EGCLGDGYCPPSCTC-----------------TGTVVRCSRNQLKEIPRGIPAETSELYLESNEIEQIHYERIRHLRSLTRLDLSNNQITILSNYTFANLTKLSTLIISYNKLQCLQRHALSGLNNLRVLSLHGNRISMLPEGSFEDLKSLTHIALGSNPLYCDCGLKWFSDWIKLDYVEPGIARCAEPEQMKDKLILSTPSSSFVCRGRVRND-ILAKCNACFEQPCQNQAQCVALPQREYQCLCQPGYHGKHCEFMIDACYGNPCRNNATCTVLEE-GRFSCQCAPGYTGARCETNIDDCLGEIKCQNNATCIDGVESYKCECQPGFSGEFCDTKIQFCSPEFNPCANGAKC-MDHFTHYSCDCQAGFHGTNCTDNIDDCQNHMCQNGGTCVDGINDYQCRCPDDYTGKYCE----------------GHNMISMMYPQT-------SPCQNHEC-KHGVCFQPNA-QGSDYLCRCHPGYTGKWCEYLTSISFVHNNSFVELEPLR---TRPEANVTIVFSSAEQNGILMYDG-QDAHLAVELFNGRIRVSYDVGNHPVSTM-YSFEMVADGKYHAVELLAIKKNFTL----RVDRGLARSIINEGSNDYLKLTTPMFLGGLPVDPAQQAYKNWQIRNLTSFKGCMKEVWINHKLVDFGN-AQRQQKITPGCALLEGEQQEEEDDEQDFMDETPHIKEEPVDPCLENKCRRGSRC--------VPNSNARDGYQCKCKHGQRGRYCDQGEGSTEPPTVTAASTCRKEQVREYYTENDCRSRQPLKYAKCVGGC---------------------GNQCCAAKIVRRRKVRMVCSNNRKYIKNLDIVRKCGCTKKC 1479 HSP 2 Score: 181.03 bits (458), Expect = 2.315e-45 Identity = 135/468 (28.85%), Postives = 219/468 (46.79%), Query Frame = 1 Query: 1504 VPKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNL--------KKLPPIY---------------SIHEAPNLMKLDLSFNQIETLEPDVFTRSSKLREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQFHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCDGNASVYHSVDSCHETNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVLSFNKLQ------------------------CIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKC 2766 + + CP C+C+ V+CS+ GL S+P I + L + NNL ++L + S + +L +LD+S N I T+ VF + LR + L++N++ C++ FK L ELEIL L+ N ++ + + F L+ L L+ N F CDC L W+S + R+ + R CQSP LK + L D+ F C G H+ C NS C + VDC + L +P +P DTTEL L++N + L SF + L+ + LS N I+ + + L+ LK L TLVL NK++ CI +DAF++LH+L +L L N++ ++ F+ +K++ + L +NPF CDCN++WL + ++ + C SP++M + I + F CS + ++ +C Sbjct: 68 ITEARCPRVCSCTGLNVDCSHRGLTSVPRKISADVERLELQGNNLTVIYETDFQRLTKLRMLQLTDNQIHTIERNSFQDLVSLERLDISNNVITTVGRRVFKGAQSLRSLQLDNNQITCLDEHAFKGLVELEILTLNNNNLTSLPHNIFGGLGRLRALRLSDNPFACDCHLSWLSRFLRSATRLAPYTR---CQSPSQLKGQNVADLHDQEFKCSG--LTEHAPMECGAENSCPHPCRCAD-----------------GIVDCREKSLTSVPVTLPDDTTELRLEQNFITELPPKSFSSFRRLRRIDLSNNNISRIAHDALSGLKQLTTLVLYGNKIKDLPSGVFKGLGSLQLLLLNANEISCIRKDAFRDLHSLSLLSLYDNNIQSLANGTFDAMKSIKTVHLAKNPFICDCNLRWLADYLHKNPIETSGARCESPKRMHRRRIESLREEKFKCSWDELRMKLSGEC 513
BLAST of slit1 vs. Ensembl Fly
Match: sli (gene:FBgn0264089 transcript:FBtr0330732) HSP 1 Score: 731.865 bits (1888), Expect = 0.000e+0 Identity = 483/1510 (31.99%), Postives = 749/1510 (49.60%), Query Frame = 1 Query: 157 CPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQLQCINDNIFNKLGMLKYLLLEGNKIFWISEAI-SKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCAS-EDKANLENMCPSDKKVCSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQIYNFQCN-KEFESKYETHDEECVPKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLPPIYSIHEAPNLMKLDLSFNQIETLEPDVFTRSSKLREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQFHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCDGNASVYHSVDSCHETNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVLSFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNL-CVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCN-DINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNSIYF-QKNSYIEISPPSRGFLMPRGNISLNFMTNSSSGLILYQGSEQTYLVVNLYQGHLRISYNLGDKVVGPEGYSMGMVNDSRLHALHIEIVGENLTMYLNGRVHCTLKSKYLN--SNRYMALDTGIFIGGAPHKILQSAIMKFHIETDSEFIGCMESLLINRKKIDFSKYLPKSINVHPGCGKLISTVVASDLKIPSIMXXXXXXXXXXXXXXXNNVCKRPGICKNQGVCEVLPGSKKK--FRCICKNGFVGRKCKKRKS-------MCHGTFTESHIVDPDSQGTCKSIERFQYRICTGTCSTSRMQSDVIKNFYHKPIDPSSSRCCQPTKYSTHEIQFKCENGRVYRRAANLPKNCKCVANC 4635 CP C C +V+C HR +VP + RL L GN + VI ++DF +L L++LQL++N+I +IE +F +LV+L +L ++ N+I + +F L+ L L +NQ+ C++++ F L L+ L L N + + I L L+ LR+ N C+CHL+WL+ FL+ + + A C +P +L ++ L EF+C+ + A +E C ++ C C C+ DGIV C K L+++P LP + EL L+ N IT L S ++ L++I L+ N + I A GL QLT++ L N +K + F GL SL L L N+I C+ KD L LL L +N + SL TF +++ ++L NP CDC + W+ DY+ T A C P+ + + +L+ F+C+ E K EC CP C C V+C+ GL +P I T L+++ N L ++ P+L+KL+L NQ+ +EP+ F +S ++E+ L N++K I+N+ F L +L+ L L +N ISC++ SF++ +L L L N F+C+C L W +EW R G R C +P ++D I L F C S + C C+ T V CS QLKEIP IP +T+ELYL+ N ++ ++ ++L +L L LS N IT L+ L L TL++S+NKLQC+ + A L+NL+VL L N +S +P +F DLK+L +IALG NP +CDC +KW + + +++ GI+ CA PE+MK K I + + F+C + I AKC++C ++PC++ +C + YQC C +HGK+C+ I+AC+G PCRN C ++ G + C C G+ G +C N+DDC+G + C N ATC D SY C+CQ GF G++CD K +C+ + NPC N +C + Y C C G+ G C N DDC+ + C++ CVD NDY+C C Y GK+CE N M Y S C HEC +G+C + + C+C PGY+G C+YL SI F NS++E+ P P N+++ F + +G+++Y G + +L V L+ G +R+SY++G+ V YS MV D + HA+ + + +N T+ RV L +N SN Y+ L T +F+GG P Q A + I + F GCM+ + IN K +DF + + PGC L + M I + N C+R C +P S + ++C CK+G GR C + + T + + + ++ C+S + +Y C G C ++CC +++ C N R Y + ++ + C C C Sbjct: 73 CPRVCSCTGLNVDCSHRGLTSVPRKISADVERLELQGNNLTVIYETDFQRLTKLRMLQLTDNQIHTIERNSFQDLVSLERLDISNNVITTVGRRVFKGAQSLRSLQLDNNQITCLDEHAFKGLVELEILTLNNNNLTSLPHNIFGGLGRLRALRLSDNPFACDCHLSWLSRFLRSATRL----APYTRCQSPSQLKGQNVADLHDQEFKCSGLTEHAPME--CGAENS-CPHPCRCA-DGIVDCREKSLTSVPVTLPDDTTELRLEQNFITELPPKSFSSFRRLRRIDLSNNNISRIAHDALSGLKQLTTLVLYGNKIKDLPSGVFKGLGSLQLLLLNANEISCIRKDAFRDLHSLSLLSLYDNNIQSLANGTFDAMKSIKTVHLAKNPFICDCNLRWLADYLHKNPIETSGARCESPKRMHRRRIESLREEKFKCSWDELRMKLSG---ECRMDSDCPAMCHCEGTTVDCTGRGLKEIPRDIPLHTTELLLNDNELGRISSDGLFGRLPHLVKLELKRNQLTGIEPNAFEGASHIQELQLGENKIKEISNKMFLGLHQLKTLNLYDNQISCVMPGSFEHLNSLTSLNLASNPFNCNCHLAWFAEWLRKKSLNGGAAR---CGAPSKVRDVQIKDLPHSEFKCSSENS-----EGCLGDGYCPPSCTC-----------------TGTVVRCSRNQLKEIPRGIPAETSELYLESNEIEQIHYERIRHLRSLTRLDLSNNQITILSNYTFANLTKLSTLIISYNKLQCLQRHALSGLNNLRVLSLHGNRISMLPEGSFEDLKSLTHIALGSNPLYCDCGLKWFSDWIKLDYVEPGIARCAEPEQMKDKLILSTPSSSFVCRGRVRND-ILAKCNACFEQPCQNQAQCVALPQREYQCLCQPGYHGKHCEFMIDACYGNPCRNNATCTVLEE-GRFSCQCAPGYTGARCETNIDDCLGEIKCQNNATCIDGVESYKCECQPGFSGEFCDTKIQFCSPEFNPCANGAKC-MDHFTHYSCDCQAGFHGTNCTDNIDDCQNHMCQNGGTCVDGINDYQCRCPDDYTGKYCE----------------GHNMISMMYPQT-------SPCQNHEC-KHGVCFQPNA-QGSDYLCRCHPGYTGKWCEYLTSISFVHNNSFVELEPLR---TRPEANVTIVFSSAEQNGILMYDG-QDAHLAVELFNGRIRVSYDVGNHPVSTM-YSFEMVADGKYHAVELLAIKKNFTL----RVDRGLARSIINEGSNDYLKLTTPMFLGGLPVDPAQQAYKNWQIRNLTSFKGCMKEVWINHKLVDFGN-AQRQQKITPGCALLEGEQQEEEDDEQDFMDETPHIKEEPVDPCLENKCRRGSRC--------VPNSNARDGYQCKCKHGQRGRYCDQGEGSTEPPTVTAASTCRKEQVREYYTENDCRSRQPLKYAKCVGGC---------------------GNQCCAAKIVRRRKVRMVCSNNRKYIKNLDIVRKCGCTKKC 1479 HSP 2 Score: 181.03 bits (458), Expect = 2.315e-45 Identity = 135/468 (28.85%), Postives = 219/468 (46.79%), Query Frame = 1 Query: 1504 VPKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNL--------KKLPPIY---------------SIHEAPNLMKLDLSFNQIETLEPDVFTRSSKLREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQFHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCDGNASVYHSVDSCHETNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVLSFNKLQ------------------------CIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKC 2766 + + CP C+C+ V+CS+ GL S+P I + L + NNL ++L + S + +L +LD+S N I T+ VF + LR + L++N++ C++ FK L ELEIL L+ N ++ + + F L+ L L+ N F CDC L W+S + R+ + R CQSP LK + L D+ F C G H+ C NS C + VDC + L +P +P DTTEL L++N + L SF + L+ + LS N I+ + + L+ LK L TLVL NK++ CI +DAF++LH+L +L L N++ ++ F+ +K++ + L +NPF CDCN++WL + ++ + C SP++M + I + F CS + ++ +C Sbjct: 68 ITEARCPRVCSCTGLNVDCSHRGLTSVPRKISADVERLELQGNNLTVIYETDFQRLTKLRMLQLTDNQIHTIERNSFQDLVSLERLDISNNVITTVGRRVFKGAQSLRSLQLDNNQITCLDEHAFKGLVELEILTLNNNNLTSLPHNIFGGLGRLRALRLSDNPFACDCHLSWLSRFLRSATRLAPYTR---CQSPSQLKGQNVADLHDQEFKCSG--LTEHAPMECGAENSCPHPCRCAD-----------------GIVDCREKSLTSVPVTLPDDTTELRLEQNFITELPPKSFSSFRRLRRIDLSNNNISRIAHDALSGLKQLTTLVLYGNKIKDLPSGVFKGLGSLQLLLLNANEISCIRKDAFRDLHSLSLLSLYDNNIQSLANGTFDAMKSIKTVHLAKNPFICDCNLRWLADYLHKNPIETSGARCESPKRMHRRRIESLREEKFKCSWDELRMKLSGEC 513
BLAST of slit1 vs. Ensembl Fly
Match: sli (gene:FBgn0264089 transcript:FBtr0330734) HSP 1 Score: 726.857 bits (1875), Expect = 0.000e+0 Identity = 486/1535 (31.66%), Postives = 751/1535 (48.93%), Query Frame = 1 Query: 157 CPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQLQCINDNIFNKLGMLKYLLLEGNKIFWISEAISK-------------------------LKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCAS-EDKANLENMCPSDKKVCSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQIYNFQCN-KEFESKYETHDEECVPKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLPPIYSIHEAPNLMKLDLSFNQIETLEPDVFTRSSKLREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQFHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCDGNASVYHSVDSCHETNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVLSFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNL-CVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCN-DINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSY-CKCKPGYSGHLCQYLNSIYF-QKNSYIEISPPSRGFLMPRGNISLNFMTNSSSGLILYQGSEQTYLVVNLYQGHLRISYNLGDKVVGPEGYSMGMVNDSRLHALHIEIVGENLTMYLNGRVHCTLKSKYLN--SNRYMALDTGIFIGGAPHKILQSAIMKFHIETDSEFIGCMESLLINRKKIDFSKYLPKSINVHPGCGKLISTVVASDLKIPSIMXXXXXXXXXXXXXXXNNVCKRPGICKNQGVCEVLPGSKKK--FRCICKNGFVGRKCKKRKS-------MCHGTFTESHIVDPDSQGTCKSIERFQYRICTGTCSTSRMQSDVIKNFYHKPIDPSSSRCCQPTKYSTHEIQFKCENGRVYRRAANLPKNCKCVANC 4635 CP C C +V+C HR +VP + RL L GN + VI ++DF +L L++LQL++N+I +IE +F +LV+L +LRLN N ++ +P++ + L +LD+ +N + + +F L+ L L+ N+I + E K L L+ LR+ N C+CHL+WL+ FL+ + + A C +P +L ++ L EF+C+ + A +E C ++ C C C+ DGIV C K L+++P LP + EL L+ N IT L S ++ L++I L+ N + I A GL QLT++ L N +K + F GL SL L L N+I C+ KD L LL L +N + SL TF +++ ++L NP CDC + W+ DY+ T A C P+ + + +L+ F+C+ E K EC CP C C V+C+ GL +P I T L+++ N L ++ P+L+KL+L NQ+ +EP+ F +S ++E+ L N++K I+N+ F L +L+ L L +N ISC++ SF++ +L L L N F+C+C L W +EW R G R C +P ++D I L F C S + C C+ T V CS QLKEIP IP +T+ELYL+ N ++ ++ ++L +L L LS N IT L+ L L TL++S+NKLQC+ + A L+NL+VL L N +S +P +F DLK+L +IALG NP +CDC +KW + + +++ GI+ CA PE+MK K I + + F+C + I AKC++C ++PC++ +C + YQC C +HGK+C+ I+AC+G PCRN C ++ G + C C G+ G +C N+DDC+G + C N ATC D SY C+CQ GF G++CD K +C+ + NPC N +C + Y C C G+ G C N DDC+ + C++ CVD NDY+C C Y GK+CE N M Y S C HEC +G+C N Y C+C PGY+G C+YL SI F NS++E+ P P N+++ F + +G+++Y G + +L V L+ G +R+SY++G+ V YS MV D + HA+ + + +N T+ RV L +N SN Y+ L T +F+GG P Q A + I + F GCM+ + IN K +DF + + PGC L + M I + N C+R C +P S + ++C CK+G GR C + + T + + + ++ C+S + +Y C G C ++CC +++ C N R Y + ++ + C C C Sbjct: 73 CPRVCSCTGLNVDCSHRGLTSVPRKISADVERLELQGNNLTVIYETDFQRLTKLRMLQLTDNQIHTIERNSFQDLVSLERLRLNNNRLKAIPENFVTSSASLLRLDISNNVITTVGRRVFKGAQSLRSLQLDNNQITCLDEHAFKGLVELEILTLNNNNLTSLPHNIFGGLGRLRALRLSDNPFACDCHLSWLSRFLRSATRL----APYTRCQSPSQLKGQNVADLHDQEFKCSGLTEHAPME--CGAENS-CPHPCRCA-DGIVDCREKSLTSVPVTLPDDTTELRLEQNFITELPPKSFSSFRRLRRIDLSNNNISRIAHDALSGLKQLTTLVLYGNKIKDLPSGVFKGLGSLQLLLLNANEISCIRKDAFRDLHSLSLLSLYDNNIQSLANGTFDAMKSIKTVHLAKNPFICDCNLRWLADYLHKNPIETSGARCESPKRMHRRRIESLREEKFKCSWDELRMKLSG---ECRMDSDCPAMCHCEGTTVDCTGRGLKEIPRDIPLHTTELLLNDNELGRISSDGLFGRLPHLVKLELKRNQLTGIEPNAFEGASHIQELQLGENKIKEISNKMFLGLHQLKTLNLYDNQISCVMPGSFEHLNSLTSLNLASNPFNCNCHLAWFAEWLRKKSLNGGAAR---CGAPSKVRDVQIKDLPHSEFKCSSENS-----EGCLGDGYCPPSCTC-----------------TGTVVRCSRNQLKEIPRGIPAETSELYLESNEIEQIHYERIRHLRSLTRLDLSNNQITILSNYTFANLTKLSTLIISYNKLQCLQRHALSGLNNLRVLSLHGNRISMLPEGSFEDLKSLTHIALGSNPLYCDCGLKWFSDWIKLDYVEPGIARCAEPEQMKDKLILSTPSSSFVCRGRVRND-ILAKCNACFEQPCQNQAQCVALPQREYQCLCQPGYHGKHCEFMIDACYGNPCRNNATCTVLEE-GRFSCQCAPGYTGARCETNIDDCLGEIKCQNNATCIDGVESYKCECQPGFSGEFCDTKIQFCSPEFNPCANGAKC-MDHFTHYSCDCQAGFHGTNCTDNIDDCQNHMCQNGGTCVDGINDYQCRCPDDYTGKYCE----------------GHNMISMMYPQT-------SPCQNHEC-KHGVCFQ--PNAQGSDYLCRCHPGYTGKWCEYLTSISFVHNNSFVELEPLR---TRPEANVTIVFSSAEQNGILMYDG-QDAHLAVELFNGRIRVSYDVGNHPVSTM-YSFEMVADGKYHAVELLAIKKNFTL----RVDRGLARSIINEGSNDYLKLTTPMFLGGLPVDPAQQAYKNWQIRNLTSFKGCMKEVWINHKLVDFGN-AQRQQKITPGCALLEGEQQEEEDDEQDFMDETPHIKEEPVDPCLENKCRRGSRC--------VPNSNARDGYQCKCKHGQRGRYCDQGEGSTEPPTVTAASTCRKEQVREYYTENDCRSRQPLKYAKCVGGC---------------------GNQCCAAKIVRRRKVRMVCSNNRKYIKNLDIVRKCGCTKKC 1503 HSP 2 Score: 174.096 bits (440), Expect = 5.066e-43 Identity = 135/492 (27.44%), Postives = 218/492 (44.31%), Query Frame = 1 Query: 1504 VPKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLK-----------------------------------------------KLPPIYSIHEAPNLMKLDLSFNQIETLEPDVFTRSSKLREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQFHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCDGNASVYHSVDSCHETNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVLSFNKLQ------------------------CIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKC 2766 + + CP C+C+ V+CS+ GL S+P I + L + NNL K P + + +L++LD+S N I T+ VF + LR + L++N++ C++ FK L ELEIL L+ N ++ + + F L+ L L+ N F CDC L W+S + R+ + R CQSP LK + L D+ F C G H+ C NS C + VDC + L +P +P DTTEL L++N + L SF + L+ + LS N I+ + + L+ LK L TLVL NK++ CI +DAF++LH+L +L L N++ ++ F+ +K++ + L +NPF CDCN++WL + ++ + C SP++M + I + F CS + ++ +C Sbjct: 68 ITEARCPRVCSCTGLNVDCSHRGLTSVPRKISADVERLELQGNNLTVIYETDFQRLTKLRMLQLTDNQIHTIERNSFQDLVSLERLRLNNNRLKAIPENFVTSSASLLRLDISNNVITTVGRRVFKGAQSLRSLQLDNNQITCLDEHAFKGLVELEILTLNNNNLTSLPHNIFGGLGRLRALRLSDNPFACDCHLSWLSRFLRSATRLAPYTR---CQSPSQLKGQNVADLHDQEFKCSG--LTEHAPMECGAENSCPHPCRCAD-----------------GIVDCREKSLTSVPVTLPDDTTELRLEQNFITELPPKSFSSFRRLRRIDLSNNNISRIAHDALSGLKQLTTLVLYGNKIKDLPSGVFKGLGSLQLLLLNANEISCIRKDAFRDLHSLSLLSLYDNNIQSLANGTFDAMKSIKTVHLAKNPFICDCNLRWLADYLHKNPIETSGARCESPKRMHRRRIESLREEKFKCSWDELRMKLSGEC 537
BLAST of slit1 vs. Ensembl Fly
Match: sli (gene:FBgn0264089 transcript:FBtr0330733) HSP 1 Score: 726.087 bits (1873), Expect = 0.000e+0 Identity = 484/1534 (31.55%), Postives = 751/1534 (48.96%), Query Frame = 1 Query: 157 CPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQLQCINDNIFNKLGMLKYLLLEGNKIFWISEAISK-------------------------LKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCAS-EDKANLENMCPSDKKVCSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQIYNFQCN-KEFESKYETHDEECVPKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLPPIYSIHEAPNLMKLDLSFNQIETLEPDVFTRSSKLREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQFHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCDGNASVYHSVDSCHETNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVLSFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNL-CVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCN-DINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNSIYF-QKNSYIEISPPSRGFLMPRGNISLNFMTNSSSGLILYQGSEQTYLVVNLYQGHLRISYNLGDKVVGPEGYSMGMVNDSRLHALHIEIVGENLTMYLNGRVHCTLKSKYLN--SNRYMALDTGIFIGGAPHKILQSAIMKFHIETDSEFIGCMESLLINRKKIDFSKYLPKSINVHPGCGKLISTVVASDLKIPSIMXXXXXXXXXXXXXXXNNVCKRPGICKNQGVCEVLPGSKKK--FRCICKNGFVGRKCKKRKS-------MCHGTFTESHIVDPDSQGTCKSIERFQYRICTGTCSTSRMQSDVIKNFYHKPIDPSSSRCCQPTKYSTHEIQFKCENGRVYRRAANLPKNCKCVANC 4635 CP C C +V+C HR +VP + RL L GN + VI ++DF +L L++LQL++N+I +IE +F +LV+L +LRLN N ++ +P++ + L +LD+ +N + + +F L+ L L+ N+I + E K L L+ LR+ N C+CHL+WL+ FL+ + + A C +P +L ++ L EF+C+ + A +E C ++ C C C+ DGIV C K L+++P LP + EL L+ N IT L S ++ L++I L+ N + I A GL QLT++ L N +K + F GL SL L L N+I C+ KD L LL L +N + SL TF +++ ++L NP CDC + W+ DY+ T A C P+ + + +L+ F+C+ E K EC CP C C V+C+ GL +P I T L+++ N L ++ P+L+KL+L NQ+ +EP+ F +S ++E+ L N++K I+N+ F L +L+ L L +N ISC++ SF++ +L L L N F+C+C L W +EW R G R C +P ++D I L F C S + C C+ T V CS QLKEIP IP +T+ELYL+ N ++ ++ ++L +L L LS N IT L+ L L TL++S+NKLQC+ + A L+NL+VL L N +S +P +F DLK+L +IALG NP +CDC +KW + + +++ GI+ CA PE+MK K I + + F+C + I AKC++C ++PC++ +C + YQC C +HGK+C+ I+AC+G PCRN C ++ G + C C G+ G +C N+DDC+G + C N ATC D SY C+CQ GF G++CD K +C+ + NPC N +C + Y C C G+ G C N DDC+ + C++ CVD NDY+C C Y GK+CE N M Y S C HEC +G+C + + C+C PGY+G C+YL SI F NS++E+ P P N+++ F + +G+++Y G + +L V L+ G +R+SY++G+ V YS MV D + HA+ + + +N T+ RV L +N SN Y+ L T +F+GG P Q A + I + F GCM+ + IN K +DF + + PGC L + M I + N C+R C +P S + ++C CK+G GR C + + T + + + ++ C+S + +Y C G C ++CC +++ C N R Y + ++ + C C C Sbjct: 73 CPRVCSCTGLNVDCSHRGLTSVPRKISADVERLELQGNNLTVIYETDFQRLTKLRMLQLTDNQIHTIERNSFQDLVSLERLRLNNNRLKAIPENFVTSSASLLRLDISNNVITTVGRRVFKGAQSLRSLQLDNNQITCLDEHAFKGLVELEILTLNNNNLTSLPHNIFGGLGRLRALRLSDNPFACDCHLSWLSRFLRSATRL----APYTRCQSPSQLKGQNVADLHDQEFKCSGLTEHAPME--CGAENS-CPHPCRCA-DGIVDCREKSLTSVPVTLPDDTTELRLEQNFITELPPKSFSSFRRLRRIDLSNNNISRIAHDALSGLKQLTTLVLYGNKIKDLPSGVFKGLGSLQLLLLNANEISCIRKDAFRDLHSLSLLSLYDNNIQSLANGTFDAMKSIKTVHLAKNPFICDCNLRWLADYLHKNPIETSGARCESPKRMHRRRIESLREEKFKCSWDELRMKLSG---ECRMDSDCPAMCHCEGTTVDCTGRGLKEIPRDIPLHTTELLLNDNELGRISSDGLFGRLPHLVKLELKRNQLTGIEPNAFEGASHIQELQLGENKIKEISNKMFLGLHQLKTLNLYDNQISCVMPGSFEHLNSLTSLNLASNPFNCNCHLAWFAEWLRKKSLNGGAAR---CGAPSKVRDVQIKDLPHSEFKCSSENS-----EGCLGDGYCPPSCTC-----------------TGTVVRCSRNQLKEIPRGIPAETSELYLESNEIEQIHYERIRHLRSLTRLDLSNNQITILSNYTFANLTKLSTLIISYNKLQCLQRHALSGLNNLRVLSLHGNRISMLPEGSFEDLKSLTHIALGSNPLYCDCGLKWFSDWIKLDYVEPGIARCAEPEQMKDKLILSTPSSSFVCRGRVRND-ILAKCNACFEQPCQNQAQCVALPQREYQCLCQPGYHGKHCEFMIDACYGNPCRNNATCTVLEE-GRFSCQCAPGYTGARCETNIDDCLGEIKCQNNATCIDGVESYKCECQPGFSGEFCDTKIQFCSPEFNPCANGAKC-MDHFTHYSCDCQAGFHGTNCTDNIDDCQNHMCQNGGTCVDGINDYQCRCPDDYTGKYCE----------------GHNMISMMYPQT-------SPCQNHEC-KHGVCFQPNA-QGSDYLCRCHPGYTGKWCEYLTSISFVHNNSFVELEPLR---TRPEANVTIVFSSAEQNGILMYDG-QDAHLAVELFNGRIRVSYDVGNHPVSTM-YSFEMVADGKYHAVELLAIKKNFTL----RVDRGLARSIINEGSNDYLKLTTPMFLGGLPVDPAQQAYKNWQIRNLTSFKGCMKEVWINHKLVDFGN-AQRQQKITPGCALLEGEQQEEEDDEQDFMDETPHIKEEPVDPCLENKCRRGSRC--------VPNSNARDGYQCKCKHGQRGRYCDQGEGSTEPPTVTAASTCRKEQVREYYTENDCRSRQPLKYAKCVGGC---------------------GNQCCAAKIVRRRKVRMVCSNNRKYIKNLDIVRKCGCTKKC 1503 HSP 2 Score: 175.252 bits (443), Expect = 1.338e-43 Identity = 135/492 (27.44%), Postives = 218/492 (44.31%), Query Frame = 1 Query: 1504 VPKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLK-----------------------------------------------KLPPIYSIHEAPNLMKLDLSFNQIETLEPDVFTRSSKLREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQFHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCDGNASVYHSVDSCHETNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVLSFNKLQ------------------------CIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKC 2766 + + CP C+C+ V+CS+ GL S+P I + L + NNL K P + + +L++LD+S N I T+ VF + LR + L++N++ C++ FK L ELEIL L+ N ++ + + F L+ L L+ N F CDC L W+S + R+ + R CQSP LK + L D+ F C G H+ C NS C + VDC + L +P +P DTTEL L++N + L SF + L+ + LS N I+ + + L+ LK L TLVL NK++ CI +DAF++LH+L +L L N++ ++ F+ +K++ + L +NPF CDCN++WL + ++ + C SP++M + I + F CS + ++ +C Sbjct: 68 ITEARCPRVCSCTGLNVDCSHRGLTSVPRKISADVERLELQGNNLTVIYETDFQRLTKLRMLQLTDNQIHTIERNSFQDLVSLERLRLNNNRLKAIPENFVTSSASLLRLDISNNVITTVGRRVFKGAQSLRSLQLDNNQITCLDEHAFKGLVELEILTLNNNNLTSLPHNIFGGLGRLRALRLSDNPFACDCHLSWLSRFLRSATRLAPYTR---CQSPSQLKGQNVADLHDQEFKCSG--LTEHAPMECGAENSCPHPCRCAD-----------------GIVDCREKSLTSVPVTLPDDTTELRLEQNFITELPPKSFSSFRRLRRIDLSNNNISRIAHDALSGLKQLTTLVLYGNKIKDLPSGVFKGLGSLQLLLLNANEISCIRKDAFRDLHSLSLLSLYDNNIQSLANGTFDAMKSIKTVHLAKNPFICDCNLRWLADYLHKNPIETSGARCESPKRMHRRRIESLREEKFKCSWDELRMKLSGEC 537
BLAST of slit1 vs. Ensembl Zebrafish
Match: slit2 (slit homolog 2 (Drosophila) [Source:ZFIN;Acc:ZDB-GENE-010306-3]) HSP 1 Score: 681.789 bits (1758), Expect = 0.000e+0 Identity = 456/1378 (33.09%), Postives = 702/1378 (50.94%), Query Frame = 1 Query: 148 SEICPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQLQ------------------------CINDNIFNKLGMLKYLLLEGNKIFWISEA-ISKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCASEDKANLENMCPSDKKVCSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQIYNFQCN--KEFESKYETHDEECVPKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLPPIYSIHEAPNLMKLDLSFNQIETLEPDVFTRSS------------------------KLREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQFHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLC-DGNASVYHSVDSCHETNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVLSFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGN-YECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYC-NDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNSIYF-QKNSYIEISPPSRGFLMPRGNISLNFMTNSSSGLILYQGSEQTYLVVNLYQGHLRISYNLGDKVVGPEG-YSMGMVNDSRLHALHIEIVGENLTMYLNGRVHCTLKSKYLNSNRYMALDTGIFIGGAPHKILQSAIMKFHIETDSEFIGCMESLLINRKKIDFSKYLPKSINVHPGC 4113 ++ CP+ C C +V+C + +VP + RL+L N + I K+DF L NL+VLQL N+I+SIE GAF +L L +LRLNRN +Q LP+ LF TKL +LDL +NQ+Q CI D F L L+ L L N I +S A + + L+ R+H N L C+C++AWL+ +L+Q+ + G ++ C P L I+ + EF C ++ C + C CS++ +V C GK L+ IP +LP + E+ L+ N+I I+ G + YK L++I L+ N + ++ +F GL L S+ L N + + K F GL SL L L N I CL D+ + + L+LL L +NKL ++ K TF ++ L+L NP CDC + W+ DY++D T A C PR L + ++ F+C+ +++ SK +C ++CP KC C V+CS L +P+ I T L +++N L + P L K++LS N+I +E F +S LR + L SNR+ C+NN +F L + +L L +N I+ + +F +L L L N F+C+C L W+ EW R V GN P CQSP LK+ PI + + F C +GN +SC CS T V CS+ LK +P IP+D TELYLD N + N +L + LS N I+ L+ + + + L TL+LS+N+L+CI AF L +L++L L ND++ IP AF DL +L+++ALG NP +CDC+++WL+ + + + GI+ C P M K + + F C+ D I AKC+ C+ PCK++G CS Y+C CPY F G++C++ I+AC PC+NGG C D N + CVCP GF G +C IN+DDC N C N +TC D N+Y+C C + G+ C+ K +C +++N CQ+ +C +L + C C+ G+ G+ CE + DDC+ N CK+ +QC+D N Y C+C GY G FCE P VL S C +++C N C+ D + C+C PGY G C+ L S+ F + S+++I PS + + NISL T+ +G++LY+G + ++ V LY+G LR+SY+ G P YS+ +ND H + + ++L++ ++G ++ + NS + +++GG P + +++ + S F GC+ +L IN + D +++L + V PGC Sbjct: 18 AQSCPSQCSCSGTAVDCHGQSLRSVPRNIPRNVERLDLNANNLTKITKADFAGLKNLRVLQLMENKISSIERGAFQDLQELERLRLNRNNLQVLPELLFLGTTKLFRLDLSENQIQGIPRKAFRGSTEIKNLQLDYNQISCIEDGAFRALRDLEVLTLNNNNISRLSVASFNHMPKLRTFRLHSNNLLCDCNVAWLSDWLRQRPRL---GLYTQ-CMAPPSLRGHNIAEVQKKEFMCTGP-----QSHSSCSVLQCPELCTCSNN-VVDCRGKGLTEIPTNLPETITEIRLEQNSIKIIPAGAFAPYKRLRRIDLSNNQITELASDSFQGLRSLNSLVLYGNKITELPKGLFDGLFSLQLLLLNANKINCLRVDSFQDLQNLNLLSLYDNKLQTIAKGTFSSLRAIQTLHLAQNPFMCDCHLKWLADYLQDNPIETSGARCTSPRRLANKRIGQIKSKKFRCSGVEDYRSKL---GGDCFADLACPEKCRCEGTTVDCSGQKLTKIPDHIPQYTAELRLNNNEFTVLEATGIFKKLPQLRKINLSNNKITDIEEGTFEGASGVNELILTSNRLEGVHYSMLKGLGGLRTLMLRSNRISCVNNGSFTGLSSVRLLSLYDNLITSMSPGAFDTLHSLSTLNLLANPFNCNCHLAWLGEWLRKKRIVTGN---PRCQSPYFLKEIPIQDVAVQDFACEEGNDE-----NSCSPLARCPAECSC-----------------LDTVVRCSNKGLKVLPKGIPRDVTELYLDGNEFTQV-PLELSNYKHLTLIDLSNNQISTLSNHSFSNMSELLTLILSYNRLRCIPAKAFDGLKSLRLLSLHGNDIAVIPDGAFKDLSSLSHLALGANPLYCDCHMQWLSDWVKSGYKEPGIARCTGPGDMTDKLLLTTPSKKFTCTGPV-DVSILAKCNPCLSNPCKNDGTCSNHPVDFYRCTCPYGFKGQDCEEPIHACISNPCQNGGTCHLKDGEENTHWCVCPEGFEGDECEINIDDCEDNDCENNSTCVDGINNYTCLCSPEYTGELCEDKLDFCASELNLCQHDSKC-ILTAKGFMCECTPGYTGEHCEVDFDDCEDNKCKNGAQCIDAVNGYTCVCPEGYSGLFCEFSP--PMVLP-----------------------RTSPCDHYDCANGAQCVVKDTDP----VCQCLPGYEGVHCERLVSVNFINRESFLQI--PSN-LITEQANISLQIATDEDNGVLLYKGDNE-HIAVELYRGRLRVSYDSGS--YPPSAIYSVETINDGSFHVVELVAKDQSLSLSIDGGSPKSINTA--NSPSPVPSPAPLYLGGLPQQSGLASLRQGSGRNGSSFHGCIRNLYINDQLQDLTQFLLQE-GVVPGC 1316 HSP 2 Score: 57.7658 bits (138), Expect = 1.414e-7 Identity = 40/121 (33.06%), Postives = 63/121 (52.07%), Query Frame = 1 Query: 2899 QPCRNG----GICKNIDNYGNYECVCPVGFRGPKCTINVDD-CVGNLCVNGATCNDLNN-SYSCKCQSGFRGKYCDIKFLYCNDINPCQ----NSGQCQLLPNNDYKCVCSKGWEGKQCER 3231 QPC+ G C + ++ C C G+ GP C V++ C GN C++G +C +N+ SYSC+C GF G CD + ++PCQ G+C++ C C+ G+ G+ C+R Sbjct: 1317 QPCQRSMCAHGQC-HATGQSSFSCECEAGWTGPLCDQQVNNPCDGNKCIHG-SCMAINSYSYSCRCLPGFAGVLCDEE----EQLSPCQYIACKYGRCRVSGLGKAYCECNSGYTGQSCDR 1431 HSP 3 Score: 56.225 bits (134), Expect = 4.057e-7 Identity = 41/140 (29.29%), Postives = 65/140 (46.43%), Query Frame = 1 Query: 3010 CVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQC--ERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRR---DINQSEMT 3414 C ++C +G +S+SC+C++G+ G CD + D N C + G C + + Y C C G+ G C E C+Y +CK+ V C C GY G+ C+ A + + T + +R N SE+T Sbjct: 1319 CQRSMCAHGQCHATGQSSFSCECEAGWTGPLCDQQVNNPCDGNKCIH-GSCMAINSYSYSCRCLPGFAGVLCDEEEQLSPCQYIACKYGRCRVSGLGKAYCECNSGYTGQSCDRAATESGMDPTQVRQRWRVGGNGSEIT 1457 HSP 4 Score: 53.5286 bits (127), Expect = 2.919e-6 Identity = 41/150 (27.33%), Postives = 59/150 (39.33%), Query Frame = 1 Query: 3139 PCQNS----GQCQLLPNNDYKCVCSKGWEGKQCERNTDD-CKYNSCKHESQCVDLHN-DYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISES---VCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLC 3561 PCQ S GQC + + C C GW G C++ ++ C N C H S C+ +++ Y C C PG+ G C+ E E +S C Y C +G+ K+YC+C GY+G C Sbjct: 1318 PCQRSMCAHGQCHATGQSSFSCECEAGWTGPLCDQQVNNPCDGNKCIHGS-CMAINSYSYSCRCLPGFAGVLCD----------------------------EEEQLSPCQYIACKYGRCRVSGL---------GKAYCECNSGYTGQSC 1429
BLAST of slit1 vs. Ensembl Zebrafish
Match: slit2 (slit homolog 2 (Drosophila) [Source:ZFIN;Acc:ZDB-GENE-010306-3]) HSP 1 Score: 680.248 bits (1754), Expect = 0.000e+0 Identity = 456/1378 (33.09%), Postives = 702/1378 (50.94%), Query Frame = 1 Query: 148 SEICPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQLQ------------------------CINDNIFNKLGMLKYLLLEGNKIFWISEA-ISKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCASEDKANLENMCPSDKKVCSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQIYNFQCN--KEFESKYETHDEECVPKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLPPIYSIHEAPNLMKLDLSFNQIETLEPDVFTRSS------------------------KLREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQFHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLC-DGNASVYHSVDSCHETNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVLSFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGN-YECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYC-NDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNSIYF-QKNSYIEISPPSRGFLMPRGNISLNFMTNSSSGLILYQGSEQTYLVVNLYQGHLRISYNLGDKVVGPEG-YSMGMVNDSRLHALHIEIVGENLTMYLNGRVHCTLKSKYLNSNRYMALDTGIFIGGAPHKILQSAIMKFHIETDSEFIGCMESLLINRKKIDFSKYLPKSINVHPGC 4113 ++ CP+ C C +V+C + +VP + RL+L N + I K+DF L NL+VLQL N+I+SIE GAF +L L +LRLNRN +Q LP+ LF TKL +LDL +NQ+Q CI D F L L+ L L N I +S A + + L+ R+H N L C+C++AWL+ +L+Q+ + G ++ C P L I+ + EF C ++ C + C CS++ +V C GK L+ IP +LP + E+ L+ N+I I+ G + YK L++I L+ N + ++ +F GL L S+ L N + + K F GL SL L L N I CL D+ + + L+LL L +NKL ++ K TF ++ L+L NP CDC + W+ DY++D T A C PR L + ++ F+C+ +++ SK +C ++CP KC C V+CS L +P+ I T L +++N L + P L K++LS N+I +E F +S LR + L SNR+ C+NN +F L + +L L +N I+ + +F +L L L N F+C+C L W+ EW R V GN P CQSP LK+ PI + + F C +GN +SC CS T V CS+ LK +P IP+D TELYLD N + N +L + LS N I+ L+ + + + L TL+LS+N+L+CI AF L +L++L L ND++ IP AF DL +L+++ALG NP +CDC+++WL+ + + + GI+ C P M K + + F C+ D I AKC+ C+ PCK++G CS Y+C CPY F G++C++ I+AC PC+NGG C D N + CVCP GF G +C IN+DDC N C N +TC D N+Y+C C + G+ C+ K +C +++N CQ+ +C +L + C C+ G+ G+ CE + DDC+ N CK+ +QC+D N Y C+C GY G FCE P VL S C +++C N C+ D + C+C PGY G C+ L S+ F + S+++I PS + + NISL T+ +G++LY+G + ++ V LY+G LR+SY+ G P YS+ +ND H + + ++L++ ++G ++ + NS + +++GG P + +++ + S F GC+ +L IN + D +++L + V PGC Sbjct: 18 AQSCPSQCSCSGTAVDCHGQSLRSVPRNIPRNVERLDLNANNLTKITKADFAGLKNLRVLQLMENKISSIERGAFQDLQELERLRLNRNNLQVLPELLFLGTTKLFRLDLSENQIQGIPRKAFRGSTEIKNLQLDYNQISCIEDGAFRALRDLEVLTLNNNNISRLSVASFNHMPKLRTFRLHSNNLLCDCNVAWLSDWLRQRPRL---GLYTQ-CMAPPSLRGHNIAEVQKKEFMCTGP-----QSHSSCSVLQCPELCTCSNN-VVDCRGKGLTEIPTNLPETITEIRLEQNSIKIIPAGAFAPYKRLRRIDLSNNQITELASDSFQGLRSLNSLVLYGNKITELPKGLFDGLFSLQLLLLNANKINCLRVDSFQDLQNLNLLSLYDNKLQTIAKGTFSSLRAIQTLHLAQNPFMCDCHLKWLADYLQDNPIETSGARCTSPRRLANKRIGQIKSKKFRCSGVEDYRSKL---GGDCFADLACPEKCRCEGTTVDCSGQKLTKIPDHIPQYTAELRLNNNEFTVLEATGIFKKLPQLRKINLSNNKITDIEEGTFEGASGVNELILTSNRLEGVHYSMLKGLGGLRTLMLRSNRISCVNNGSFTGLSSVRLLSLYDNLITSMSPGAFDTLHSLSTLNLLANPFNCNCHLAWLGEWLRKKRIVTGN---PRCQSPYFLKEIPIQDVAVQDFACEEGNDE-----NSCSPLARCPAECSC-----------------LDTVVRCSNKGLKVLPKGIPRDVTELYLDGNEFTQV-PLELSNYKHLTLIDLSNNQISTLSNHSFSNMSELLTLILSYNRLRCIPAKAFDGLKSLRLLSLHGNDIAVIPDGAFKDLSSLSHLALGANPLYCDCHMQWLSDWVKSGYKEPGIARCTGPGDMTDKLLLTTPSKKFTCTGPV-DVSILAKCNPCLSNPCKNDGTCSNHPVDFYRCTCPYGFKGQDCEEPIHACISNPCQNGGTCHLKDGEENTHWCVCPEGFEGDECEINIDDCEDNDCENNSTCVDGINNYTCLCSPEYTGELCEDKLDFCASELNLCQHDSKC-ILTAKGFMCECTPGYTGEHCEVDFDDCEDNKCKNGAQCIDAVNGYTCVCPEGYSGLFCEFSP--PMVLP-----------------------RTSPCDHYDCANGAQCVVKDTDP----VCQCLPGYEGVHCERLVSVNFINRESFLQI--PSN-LITEQANISLQIATDEDNGVLLYKGDNE-HIAVELYRGRLRVSYDSGS--YPPSAIYSVETINDGSFHVVELVAKDQSLSLSIDGGSPKSINTA--NSPSPVPSPAPLYLGGLPQQSGLASLRQGSGRNGSSFHGCIRNLYINDQLQDLTQFLLQE-GVVPGC 1316 HSP 2 Score: 57.3806 bits (137), Expect = 1.798e-7 Identity = 40/121 (33.06%), Postives = 63/121 (52.07%), Query Frame = 1 Query: 2899 QPCRNG----GICKNIDNYGNYECVCPVGFRGPKCTINVDD-CVGNLCVNGATCNDLNN-SYSCKCQSGFRGKYCDIKFLYCNDINPCQ----NSGQCQLLPNNDYKCVCSKGWEGKQCER 3231 QPC+ G C + ++ C C G+ GP C V++ C GN C++G +C +N+ SYSC+C GF G CD + ++PCQ G+C++ C C+ G+ G+ C+R Sbjct: 1317 QPCQRSMCAHGQC-HATGQSSFSCECEAGWTGPLCDQQVNNPCDGNKCIHG-SCMAINSYSYSCRCLPGFAGVLCDEE----EQLSPCQYIACKYGRCRVSGLGKAYCECNSGYTGQSCDR 1431 HSP 3 Score: 52.7582 bits (125), Expect = 4.283e-6 Identity = 41/150 (27.33%), Postives = 59/150 (39.33%), Query Frame = 1 Query: 3139 PCQNS----GQCQLLPNNDYKCVCSKGWEGKQCERNTDD-CKYNSCKHESQCVDLHN-DYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISES---VCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLC 3561 PCQ S GQC + + C C GW G C++ ++ C N C H S C+ +++ Y C C PG+ G C+ E E +S C Y C +G+ K+YC+C GY+G C Sbjct: 1318 PCQRSMCAHGQCHATGQSSFSCECEAGWTGPLCDQQVNNPCDGNKCIHGS-CMAINSYSYSCRCLPGFAGVLCD----------------------------EEEQLSPCQYIACKYGRCRVSGL---------GKAYCECNSGYTGQSC 1429 HSP 4 Score: 51.9878 bits (123), Expect = 7.346e-6 Identity = 34/113 (30.09%), Postives = 53/113 (46.90%), Query Frame = 1 Query: 3010 CVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQC--ERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCE 3342 C ++C +G +S+SC+C++G+ G CD + D N C + G C + + Y C C G+ G C E C+Y +CK+ V C C GY G+ C+ Sbjct: 1319 CQRSMCAHGQCHATGQSSFSCECEAGWTGPLCDQQVNNPCDGNKCIH-GSCMAINSYSYSCRCLPGFAGVLCDEEEQLSPCQYIACKYGRCRVSGLGKAYCECNSGYTGQSCD 1430
BLAST of slit1 vs. Ensembl Zebrafish
Match: slit2 (slit homolog 2 (Drosophila) [Source:ZFIN;Acc:ZDB-GENE-010306-3]) HSP 1 Score: 675.626 bits (1742), Expect = 0.000e+0 Identity = 456/1387 (32.88%), Postives = 703/1387 (50.68%), Query Frame = 1 Query: 148 SEICPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQLQ------------------------CINDNIFNKLGMLKYLLLEGNKIFWISEA-ISKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCASEDKANLENMCPSDKKVCSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQIYNFQCN--KEFESKYETHDEECVPKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLPPIYSIHEAPNLMKLDLSFNQIETLEPDVFTRSS------------------------KLREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQFHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLC-DGNASVYHSVDSCHETNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVLSFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGN-YECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGF---------RGKYCDIKFLYC-NDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNSIYF-QKNSYIEISPPSRGFLMPRGNISLNFMTNSSSGLILYQGSEQTYLVVNLYQGHLRISYNLGDKVVGPEG-YSMGMVNDSRLHALHIEIVGENLTMYLNGRVHCTLKSKYLNSNRYMALDTGIFIGGAPHKILQSAIMKFHIETDSEFIGCMESLLINRKKIDFSKYLPKSINVHPGC 4113 ++ CP+ C C +V+C + +VP + RL+L N + I K+DF L NL+VLQL N+I+SIE GAF +L L +LRLNRN +Q LP+ LF TKL +LDL +NQ+Q CI D F L L+ L L N I +S A + + L+ R+H N L C+C++AWL+ +L+Q+ + G ++ C P L I+ + EF C ++ C + C CS++ +V C GK L+ IP +LP + E+ L+ N+I I+ G + YK L++I L+ N + ++ +F GL L S+ L N + + K F GL SL L L N I CL D+ + + L+LL L +NKL ++ K TF ++ L+L NP CDC + W+ DY++D T A C PR L + ++ F+C+ +++ SK +C ++CP KC C V+CS L +P+ I T L +++N L + P L K++LS N+I +E F +S LR + L SNR+ C+NN +F L + +L L +N I+ + +F +L L L N F+C+C L W+ EW R V GN P CQSP LK+ PI + + F C +GN +SC CS T V CS+ LK +P IP+D TELYLD N + N +L + LS N I+ L+ + + + L TL+LS+N+L+CI AF L +L++L L ND++ IP AF DL +L+++ALG NP +CDC+++WL+ + + + GI+ C P M K + + F C+ D I AKC+ C+ PCK++G CS Y+C CPY F G++C++ I+AC PC+NGG C D N + CVCP GF G +C IN+DDC N C N +TC D N+Y+C C + +G+ C+ K +C +++N CQ+ +C +L + C C+ G+ G+ CE + DDC+ N CK+ +QC+D N Y C+C GY G FCE P VL S C +++C N C+ D + C+C PGY G C+ L S+ F + S+++I PS + + NISL T+ +G++LY+G + ++ V LY+G LR+SY+ G P YS+ +ND H + + ++L++ ++G ++ + NS + +++GG P + +++ + S F GC+ +L IN + D +++L + V PGC Sbjct: 18 AQSCPSQCSCSGTAVDCHGQSLRSVPRNIPRNVERLDLNANNLTKITKADFAGLKNLRVLQLMENKISSIERGAFQDLQELERLRLNRNNLQVLPELLFLGTTKLFRLDLSENQIQGIPRKAFRGSTEIKNLQLDYNQISCIEDGAFRALRDLEVLTLNNNNISRLSVASFNHMPKLRTFRLHSNNLLCDCNVAWLSDWLRQRPRL---GLYTQ-CMAPPSLRGHNIAEVQKKEFMCTGP-----QSHSSCSVLQCPELCTCSNN-VVDCRGKGLTEIPTNLPETITEIRLEQNSIKIIPAGAFAPYKRLRRIDLSNNQITELASDSFQGLRSLNSLVLYGNKITELPKGLFDGLFSLQLLLLNANKINCLRVDSFQDLQNLNLLSLYDNKLQTIAKGTFSSLRAIQTLHLAQNPFMCDCHLKWLADYLQDNPIETSGARCTSPRRLANKRIGQIKSKKFRCSGVEDYRSKL---GGDCFADLACPEKCRCEGTTVDCSGQKLTKIPDHIPQYTAELRLNNNEFTVLEATGIFKKLPQLRKINLSNNKITDIEEGTFEGASGVNELILTSNRLEGVHYSMLKGLGGLRTLMLRSNRISCVNNGSFTGLSSVRLLSLYDNLITSMSPGAFDTLHSLSTLNLLANPFNCNCHLAWLGEWLRKKRIVTGN---PRCQSPYFLKEIPIQDVAVQDFACEEGNDE-----NSCSPLARCPAECSC-----------------LDTVVRCSNKGLKVLPKGIPRDVTELYLDGNEFTQV-PLELSNYKHLTLIDLSNNQISTLSNHSFSNMSELLTLILSYNRLRCIPAKAFDGLKSLRLLSLHGNDIAVIPDGAFKDLSSLSHLALGANPLYCDCHMQWLSDWVKSGYKEPGIARCTGPGDMTDKLLLTTPSKKFTCTGPV-DVSILAKCNPCLSNPCKNDGTCSNHPVDFYRCTCPYGFKGQDCEEPIHACISNPCQNGGTCHLKDGEENTHWCVCPEGFEGDECEINIDDCEDNDCENNSTCVDGINNYTCLCSPEYTAANNNEVEKGELCEDKLDFCASELNLCQHDSKC-ILTAKGFMCECTPGYTGEHCEVDFDDCEDNKCKNGAQCIDAVNGYTCVCPEGYSGLFCEFSP--PMVLP-----------------------RTSPCDHYDCANGAQCVVKDTDP----VCQCLPGYEGVHCERLVSVNFINRESFLQI--PSN-LITEQANISLQIATDEDNGVLLYKGDNE-HIAVELYRGRLRVSYDSGS--YPPSAIYSVETINDGSFHVVELVAKDQSLSLSIDGGSPKSINTA--NSPSPVPSPAPLYLGGLPQQSGLASLRQGSGRNGSSFHGCIRNLYINDQLQDLTQFLLQE-GVVPGC 1325 HSP 2 Score: 57.3806 bits (137), Expect = 1.641e-7 Identity = 40/121 (33.06%), Postives = 63/121 (52.07%), Query Frame = 1 Query: 2899 QPCRNG----GICKNIDNYGNYECVCPVGFRGPKCTINVDD-CVGNLCVNGATCNDLNN-SYSCKCQSGFRGKYCDIKFLYCNDINPCQ----NSGQCQLLPNNDYKCVCSKGWEGKQCER 3231 QPC+ G C + ++ C C G+ GP C V++ C GN C++G +C +N+ SYSC+C GF G CD + ++PCQ G+C++ C C+ G+ G+ C+R Sbjct: 1326 QPCQRSMCAHGQC-HATGQSSFSCECEAGWTGPLCDQQVNNPCDGNKCIHG-SCMAINSYSYSCRCLPGFAGVLCDEE----EQLSPCQYIACKYGRCRVSGLGKAYCECNSGYTGQSCDR 1440 HSP 3 Score: 53.1434 bits (126), Expect = 4.110e-6 Identity = 41/150 (27.33%), Postives = 59/150 (39.33%), Query Frame = 1 Query: 3139 PCQNS----GQCQLLPNNDYKCVCSKGWEGKQCERNTDD-CKYNSCKHESQCVDLHN-DYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISES---VCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLC 3561 PCQ S GQC + + C C GW G C++ ++ C N C H S C+ +++ Y C C PG+ G C+ E E +S C Y C +G+ K+YC+C GY+G C Sbjct: 1327 PCQRSMCAHGQCHATGQSSFSCECEAGWTGPLCDQQVNNPCDGNKCIHGS-CMAINSYSYSCRCLPGFAGVLCD----------------------------EEEQLSPCQYIACKYGRCRVSGL---------GKAYCECNSGYTGQSC 1438 HSP 4 Score: 51.9878 bits (123), Expect = 7.415e-6 Identity = 34/113 (30.09%), Postives = 53/113 (46.90%), Query Frame = 1 Query: 3010 CVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQC--ERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCE 3342 C ++C +G +S+SC+C++G+ G CD + D N C + G C + + Y C C G+ G C E C+Y +CK+ V C C GY G+ C+ Sbjct: 1328 CQRSMCAHGQCHATGQSSFSCECEAGWTGPLCDQQVNNPCDGNKCIH-GSCMAINSYSYSCRCLPGFAGVLCDEEEQLSPCQYIACKYGRCRVSGLGKAYCECNSGYTGQSCD 1439
BLAST of slit1 vs. Ensembl Zebrafish
Match: slit2 (slit homolog 2 (Drosophila) [Source:ZFIN;Acc:ZDB-GENE-010306-3]) HSP 1 Score: 674.47 bits (1739), Expect = 0.000e+0 Identity = 456/1387 (32.88%), Postives = 703/1387 (50.68%), Query Frame = 1 Query: 148 SEICPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQLQ------------------------CINDNIFNKLGMLKYLLLEGNKIFWISEA-ISKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCASEDKANLENMCPSDKKVCSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQIYNFQCN--KEFESKYETHDEECVPKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLPPIYSIHEAPNLMKLDLSFNQIETLEPDVFTRSS------------------------KLREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQFHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLC-DGNASVYHSVDSCHETNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVLSFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGN-YECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGF---------RGKYCDIKFLYC-NDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNSIYF-QKNSYIEISPPSRGFLMPRGNISLNFMTNSSSGLILYQGSEQTYLVVNLYQGHLRISYNLGDKVVGPEG-YSMGMVNDSRLHALHIEIVGENLTMYLNGRVHCTLKSKYLNSNRYMALDTGIFIGGAPHKILQSAIMKFHIETDSEFIGCMESLLINRKKIDFSKYLPKSINVHPGC 4113 ++ CP+ C C +V+C + +VP + RL+L N + I K+DF L NL+VLQL N+I+SIE GAF +L L +LRLNRN +Q LP+ LF TKL +LDL +NQ+Q CI D F L L+ L L N I +S A + + L+ R+H N L C+C++AWL+ +L+Q+ + G ++ C P L I+ + EF C ++ C + C CS++ +V C GK L+ IP +LP + E+ L+ N+I I+ G + YK L++I L+ N + ++ +F GL L S+ L N + + K F GL SL L L N I CL D+ + + L+LL L +NKL ++ K TF ++ L+L NP CDC + W+ DY++D T A C PR L + ++ F+C+ +++ SK +C ++CP KC C V+CS L +P+ I T L +++N L + P L K++LS N+I +E F +S LR + L SNR+ C+NN +F L + +L L +N I+ + +F +L L L N F+C+C L W+ EW R V GN P CQSP LK+ PI + + F C +GN +SC CS T V CS+ LK +P IP+D TELYLD N + N +L + LS N I+ L+ + + + L TL+LS+N+L+CI AF L +L++L L ND++ IP AF DL +L+++ALG NP +CDC+++WL+ + + + GI+ C P M K + + F C+ D I AKC+ C+ PCK++G CS Y+C CPY F G++C++ I+AC PC+NGG C D N + CVCP GF G +C IN+DDC N C N +TC D N+Y+C C + +G+ C+ K +C +++N CQ+ +C +L + C C+ G+ G+ CE + DDC+ N CK+ +QC+D N Y C+C GY G FCE P VL S C +++C N C+ D + C+C PGY G C+ L S+ F + S+++I PS + + NISL T+ +G++LY+G + ++ V LY+G LR+SY+ G P YS+ +ND H + + ++L++ ++G ++ + NS + +++GG P + +++ + S F GC+ +L IN + D +++L + V PGC Sbjct: 18 AQSCPSQCSCSGTAVDCHGQSLRSVPRNIPRNVERLDLNANNLTKITKADFAGLKNLRVLQLMENKISSIERGAFQDLQELERLRLNRNNLQVLPELLFLGTTKLFRLDLSENQIQGIPRKAFRGSTEIKNLQLDYNQISCIEDGAFRALRDLEVLTLNNNNISRLSVASFNHMPKLRTFRLHSNNLLCDCNVAWLSDWLRQRPRL---GLYTQ-CMAPPSLRGHNIAEVQKKEFMCTGP-----QSHSSCSVLQCPELCTCSNN-VVDCRGKGLTEIPTNLPETITEIRLEQNSIKIIPAGAFAPYKRLRRIDLSNNQITELASDSFQGLRSLNSLVLYGNKITELPKGLFDGLFSLQLLLLNANKINCLRVDSFQDLQNLNLLSLYDNKLQTIAKGTFSSLRAIQTLHLAQNPFMCDCHLKWLADYLQDNPIETSGARCTSPRRLANKRIGQIKSKKFRCSGVEDYRSKL---GGDCFADLACPEKCRCEGTTVDCSGQKLTKIPDHIPQYTAELRLNNNEFTVLEATGIFKKLPQLRKINLSNNKITDIEEGTFEGASGVNELILTSNRLEGVHYSMLKGLGGLRTLMLRSNRISCVNNGSFTGLSSVRLLSLYDNLITSMSPGAFDTLHSLSTLNLLANPFNCNCHLAWLGEWLRKKRIVTGN---PRCQSPYFLKEIPIQDVAVQDFACEEGNDE-----NSCSPLARCPAECSC-----------------LDTVVRCSNKGLKVLPKGIPRDVTELYLDGNEFTQV-PLELSNYKHLTLIDLSNNQISTLSNHSFSNMSELLTLILSYNRLRCIPAKAFDGLKSLRLLSLHGNDIAVIPDGAFKDLSSLSHLALGANPLYCDCHMQWLSDWVKSGYKEPGIARCTGPGDMTDKLLLTTPSKKFTCTGPV-DVSILAKCNPCLSNPCKNDGTCSNHPVDFYRCTCPYGFKGQDCEEPIHACISNPCQNGGTCHLKDGEENTHWCVCPEGFEGDECEINIDDCEDNDCENNSTCVDGINNYTCLCSPEYTAANNNEVEKGELCEDKLDFCASELNLCQHDSKC-ILTAKGFMCECTPGYTGEHCEVDFDDCEDNKCKNGAQCIDAVNGYTCVCPEGYSGLFCEFSP--PMVLP-----------------------RTSPCDHYDCANGAQCVVKDTDP----VCQCLPGYEGVHCERLVSVNFINRESFLQI--PSN-LITEQANISLQIATDEDNGVLLYKGDNE-HIAVELYRGRLRVSYDSGS--YPPSAIYSVETINDGSFHVVELVAKDQSLSLSIDGGSPKSINTA--NSPSPVPSPAPLYLGGLPQQSGLASLRQGSGRNGSSFHGCIRNLYINDQLQDLTQFLLQE-GVVPGC 1325 HSP 2 Score: 57.7658 bits (138), Expect = 1.452e-7 Identity = 40/121 (33.06%), Postives = 63/121 (52.07%), Query Frame = 1 Query: 2899 QPCRNG----GICKNIDNYGNYECVCPVGFRGPKCTINVDD-CVGNLCVNGATCNDLNN-SYSCKCQSGFRGKYCDIKFLYCNDINPCQ----NSGQCQLLPNNDYKCVCSKGWEGKQCER 3231 QPC+ G C + ++ C C G+ GP C V++ C GN C++G +C +N+ SYSC+C GF G CD + ++PCQ G+C++ C C+ G+ G+ C+R Sbjct: 1326 QPCQRSMCAHGQC-HATGQSSFSCECEAGWTGPLCDQQVNNPCDGNKCIHG-SCMAINSYSYSCRCLPGFAGVLCDEE----EQLSPCQYIACKYGRCRVSGLGKAYCECNSGYTGQSCDR 1440 HSP 3 Score: 56.225 bits (134), Expect = 4.308e-7 Identity = 41/140 (29.29%), Postives = 65/140 (46.43%), Query Frame = 1 Query: 3010 CVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQC--ERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRR---DINQSEMT 3414 C ++C +G +S+SC+C++G+ G CD + D N C + G C + + Y C C G+ G C E C+Y +CK+ V C C GY G+ C+ A + + T + +R N SE+T Sbjct: 1328 CQRSMCAHGQCHATGQSSFSCECEAGWTGPLCDQQVNNPCDGNKCIH-GSCMAINSYSYSCRCLPGFAGVLCDEEEQLSPCQYIACKYGRCRVSGLGKAYCECNSGYTGQSCDRAATESGMDPTQVRQRWRVGGNGSEIT 1466 HSP 4 Score: 53.5286 bits (127), Expect = 3.099e-6 Identity = 41/150 (27.33%), Postives = 59/150 (39.33%), Query Frame = 1 Query: 3139 PCQNS----GQCQLLPNNDYKCVCSKGWEGKQCERNTDD-CKYNSCKHESQCVDLHN-DYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISES---VCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLC 3561 PCQ S GQC + + C C GW G C++ ++ C N C H S C+ +++ Y C C PG+ G C+ E E +S C Y C +G+ K+YC+C GY+G C Sbjct: 1327 PCQRSMCAHGQCHATGQSSFSCECEAGWTGPLCDQQVNNPCDGNKCIHGS-CMAINSYSYSCRCLPGFAGVLCD----------------------------EEEQLSPCQYIACKYGRCRVSGL---------GKAYCECNSGYTGQSC 1438
BLAST of slit1 vs. Ensembl Zebrafish
Match: slit3 (slit homolog 3 (Drosophila) [Source:ZFIN;Acc:ZDB-GENE-010306-4]) HSP 1 Score: 671.774 bits (1732), Expect = 0.000e+0 Identity = 498/1585 (31.42%), Postives = 750/1585 (47.32%), Query Frame = 1 Query: 157 CPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQLQ------------------------CINDNIFNKLGMLKYLLLEGNKIFWIS-EAISKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCASEDKANLENMCPSDKKVCSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQIYNFQCN--KEFESKYETHDEECVPKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLPPIYSIHEAPNLMKLDLSFNQIETLEPDVFTRSS----------KLREIS--------------LNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQFHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCDGNAS--VYHSVDSCHETNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVLSFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNY-GNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYC-NDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNSIYF-QKNSYIEISPPSRGFLMPRGNISLNFMTNSSSGLILYQGSEQTYLVVNLYQGHLRISYNLGDKVVGPEGYSMGMVNDSRLHALHIEIVGENLTMYLNGRVHCTLKSKYLNSNRYMALDTGIFIGGAPHKILQSAIMKFHIETDSEFIGCMESLLINRKKIDFSKYLPK-----------SINVHPGCGKLISTVVASDLKIPSIMXXXXXXXXXXXXXXXNNVCKRPGICKNQGVCEVLPGSKKKFRCICKNGFVGRKCKKR--KSMCHG--------TFTES-----HIVDPDSQGTC--------KSIERFQYR-----ICTGTCSTSRMQSDVIKNFYHKPIDPSSSRCCQPTKYSTHEIQFKCENGRVYRRAANLPKNCKCV 4626 CP+ C C V+C+ + F VP + RL+L N I I K DF L NL+VL L NN+I+ IE GAF+ L L ++RLNRN +Q LP+ LF TKL +LDL +NQ+Q CI D F L L+ L L N I I + + + L+ LR+H N L+C+CHL+WL+ +L+Q+ + A C P + + + + EF C + + C +C C C+++ IV C K L+ IP +LP +VE+ L+ N I + G S YK LK+I L++N + +I AF GL LTS+ L N + I K F GLVSL L L N I CL +T + + L+LL L +NKL ++ K F P ++ L+L NP CDC + W+ DY+ D T A C PR L + ++ F+C +++ S+ EC + CP +C C VV+CSN L +P + T L ++ N + L + + PNL K++LS N++ + F + KL+ +S L SN++ C++N TF L + +L L +N IS I +F +L + L N + CDC L W+ W + V GN P CQ P LK+ PI + F CDG S C E+ + CS T V CS+ L+ +P IPKDTTELYL+ N L S+ + NL L L LS N I+ L + + L TL+LS+N+++C+ AF L +L++L L ND+ST+P AFN L +L+++ALG NP +CDC ++WL+Q+ F + GI+ C P M + + + F C + D + +KC C+ PC++NG C +Y C CP+ + G+NC+ INAC PC NGG C G Y CVCP G+ G +C +N DDC N C N +TC D N+Y+C C ++G C+ C + CQ+ +C L + Y+C C G+ G+QCE++ +DC N C+H ++CVD N Y C+C+ G+ G FCE P VL L+N S C +C N C+ + + C+C PGY G CQ + +++F K +Y+E+S + P +IS T+ +G++LY+ + L + LYQGH+R+ Y++ K YS+ VND H + + I + L++ ++ +L L + +T ++IGG P I S + ++ F GC+ + +N + D L S+ C + V D P+ + ++ C G C +G + C+C +G+ G+ C ++ S C G TES H S TC +++ + YR ICT STSR+Q ++ SS CC PTK ++ FKC +G + + C C Sbjct: 25 CPHKCSCSGSHVDCQGQAFKTVPRGIPRNAERLDLDRNNITRITKVDFSGLKNLRVLHLENNQISVIERGAFEGLKQLERIRLNRNRLQVLPELLFQSTTKLSRLDLSENQIQAVPRKAFRGITTVKNLQLDSNHISCIEDGAFRALRDLEILTLNNNNITLIPLSSFNHMPKLRTLRLHSNNLHCDCHLSWLSDWLRQRRGL----APFTQCMAPAHMRGLNVPDVQKREFVCTGPVETEPRS-CAPQVTICPAACTCNNN-IVDCRRKGLTEIPANLPEGIVEIRLEQNMIKNIPAGAFSTYKKLKRIDLSKNQISEIAEDAFSGLRSLTSLVLYGNKIAEIPKGLFDGLVSLQLLLLNANKINCLRVNTFKDLQNLNLLSLYDNKLQTISKGLFAPLRAIKTLHLAQNPFMCDCHLKWLADYLFDNPIETSGARCSHPRRLANKRISQVKGKKFRCTGAEDYRSRLSG---ECFQDLVCPERCRCEGTVVDCSNLKLTKIPPHLPEHTTDLRLNDNEISILEATGAFKKLPNLRKINLSNNKLRDIREGAFDGAGGVLELLLTGNKLQSVSGRMFKGLIGLKTLMLRSNQISCVDNATFTGLSSVRLLSLYDNRISTIAPGAFNTLHSLSTINLLSNPYVCDCHLAWLGLWLKKTRVVSGN---PRCQKPAFLKEIPIQDVAMPDFSCDGAEENVCLPSAPRCPESCT----CS-------------------ETVVRCSNRGLRSLPKTIPKDTTELYLEGNLLTSVPK-ELPNLKQLTLLDLSNNSISHLAPLTFSNMTQLATLILSYNQIRCVPVHAFDGLRSLRLLTLHGNDLSTVPEGAFNHLTSLSHLALGANPLYCDCELRWLSQWVKAGFKEPGIARCTGPPDMADRLLLTTPLNRFQC-KGPADLNLMSKCAPCLATPCQNNGTCVSDVTGSYHCTCPFGYKGRNCEIPINACISLPCTNGGTCHLTPGLDGQYSCVCPPGYEGQQCELNPDDCEDNDCENNSTCVDGINNYTCVCPPNYKGDLCEEVVDPCLPGFDLCQHDSKCVPL-SKGYRCECLPGYVGQQCEQDYNDCLENKCQHGAECVDAINGYTCVCKEGFSGLFCE--NHPPMVL------------------LQN----TSPCDQSDCQNGSECLVME----GEPVCRCLPGYFGTKCQKMVTLHFLGKEAYVELSGTR---IRPPMHISFQVATDKDNGILLYK-EDHDPLALELYQGHIRLIYDI-SKYPPTTVYSVESVNDGLFHTVEVLIQNQTLSLVVDKGTPKSLGK--LPRPPAVDHNTQLYIGGVPSLIGTSGLRPGPFQS---FNGCLHDVRLNGELQDLGAGLAGIEGVLFGCHSCSVCDQGACRQRGDEGVTCD--CPTGHSGPLCEQMSSQDPCESSRCVH-GECVAKG---------SSYSCLCVDGYSGQFCDRQDESSACKGLHCVYGECRLTESGEPACHCQPGYSGPTCETELTCEGEAVRDYLYRQMAHKICT---STSRVQRLECRH------SCSSGLCCAPTKSRRRKLSFKCPDGSSFSEEMEITVECGCT 1512 HSP 2 Score: 289.656 bits (740), Expect = 7.392e-79 Identity = 212/655 (32.37%), Postives = 322/655 (49.16%), Query Frame = 1 Query: 154 ICPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQLQCINDNIFNKLGMLKYLLLEGNKIFWISEAI-SKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQC--ASEDKANLENMCPSDKKVCSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQ-GYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQIYNFQCNKEFESKYETHDEECVPKVS-CPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLPPIYSIHEAPNLMK---LDLSFNQIETLEPDVFTRSSKLREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQFHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCDGNASV 2094 ICP C C N V+C+ + +P L E + L N I I F LK + LS N+I+ I AF L +L L L N I +P LF L LQ L L N++ C+ N F L L L L NK+ IS+ + + L+ +K L + +N C+CHL WL +L I GA CS P RL++ IS + +F+C A + ++ L C D VC ++C C +V C +L+ IP LP + +L L DN I+IL+ G K L+KI L+ N + DI GAF G + + L N L+++ F GL+ L L L+ N I C+ T + LL L +N+++++ F +L + L+ NP CDC ++W+ ++K + C +P L+ P++++ + +F C+ E+ C+P CP CTCS VV CSN GL SLP++I T L + N L +P E PNL + LDLS N I L P F+ ++L + L+ N+++C+ F L+ L +L L N +S + +F + +L +L L N +CDC+L+W+S+W + + G R C P + D + F C G A + Sbjct: 271 ICPAACTCNNNIVDCRRKGLTEIPANLPEGIVEIRLEQNMIKNIPAGAFSTYKKLKRIDLSKNQISEIAEDAFSGLRSLTSLVLYGNKIAEIPKGLFDGLVSLQLLLLNANKINCLRVNTFKDLQNLNLLSLYDNKLQTISKGLFAPLRAIKTLHLAQNPFMCDCHLKWLADYLFDNP-IETSGAR---CSHPRRLANKRISQVKGKKFRCTGAEDYRSRLSGECFQD-LVCPERCRC-EGTVVDCSNLKLTKIPPHLPEHTTDLRLNDNEISILEATGAFKKLPNLRKINLSNNKLRDIREGAFDGAGGVLELLLTGNKLQSVSGRMFKGLIGLKTLMLRSNQISCVDNATFTGLSSVRLLSLYDNRISTIAPGAFNTLHSLSTINLLSNPYVCDCHLAWLGLWLKKTRVVSGNPRCQKPAFLKEIPIQDVAMPDFSCDGAEENV-------CLPSAPRCPESCTCSETVVRCSNRGLRSLPKTIPKDTTELYLEGNLLTSVP-----KELPNLKQLTLLDLSNNSISHLAPLTFSNMTQLATLILSYNQIRCVPVHAFDGLRSLRLLTLHGNDLSTVPEGAFNHLTSLSHLALGANPLYCDCELRWLSQWVKAGFKEPGIAR---CTGPPDMADRLLLTTPLNRFQCKGPADL 904
BLAST of slit1 vs. Ensembl Xenopus
Match: zxdc (ZXD family zinc finger C [Source:Xenbase;Acc:XB-GENE-1033000]) HSP 1 Score: 714.531 bits (1843), Expect = 0.000e+0 Identity = 510/1615 (31.58%), Postives = 764/1615 (47.31%), Query Frame = 1 Query: 157 CPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQLQ------------------------CINDNIFNKLGMLKYLLLEGNKIFWIS-EAISKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCASEDKANLENMCPSDKKVCSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQIYNFQC--NKEFESKYETHDEECVPKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLPPIYSIHEAPNLMKLDLSFNQIETLEPDVFTRSSKLREISLNSNRLK------------------------CINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQFHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCDGNAS-----VYHSVDSCHETNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNE--TSFKNLLNLKTLV-LSYNGITELNKNVLTPLKSLETLVLSFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGIC-KNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYC-NDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNSIYF-QKNSYIEISPPSRGFLMPRGNISLNFMTNSSSGLILYQGSEQTYLVVNLYQGHLRISYNLGDKVVGPEG--YSMGMVNDSRLHALHIEIVGENLTMYLNGRVHCTLKSKYLNSNRYMALDTGIFIGGAPHKILQSAIMKFHIETDS---EFIGCMESLLINRKKIDFSKYLPKSINVHPGCGKLISTVVASDLKIPSIMXXXXXXXXXXXXXXXNNVCKRPGIC----KNQGVCEVLPG--------------------------SKKKFRCICKNGFVGRKCKKRK---SMC------HGTFTESHIVDP-------------DSQGTC-----KSIERFQ--YRICTGTCSTSRMQSDVIKNFYHKPIDPSSSRCCQPTKYSTHEIQFKCENGRVYRRAANLPKNCKC 4623 CP+ C C +V+C VP + RL++ N I I K+DF L NL+VL L +N+I+ IE GAF +L L ++RLN+N +Q LP+ LF KL +LDL +NQ+Q CI D +F L L+ L L N I I + + + ++ LR+H N L C+CHL WL+ +L+Q+ I C +P L ++ + +F C C ++ C C CS++ +V C GK L+ IP +LP ++E+ L+ N+I + G + YK LK+I +++N + +I AF GL LTS+ L N + I K F GLVSL L L N I CL +T + + L+LL L +NKL ++ K F P +++ L+L NP CDC + W+ DY++D T A C PR L + ++ F+C ++++ SK+ +C + CP KC C +V+CSN + +P + T L ++ N + L + PNL K++LS N+I+ + F ++ ++E+ L N+L+ CINN+TF L + +L L +N I+ I +F ++L + L N F+C+C L W+ +W R V GN P CQ P LK+ PI + + F CDGN H + C SV V C + L+ +P IPKD TELYL+ NHL S+ + +SFK+L TL+ LS N I+ L+ + + L TL+LS+N+L+CI AF L +L+VL L ND+ST+P +F+DL +L+++ALG NP +CDCN++WL+++ + + GI+ C+ PE M + + + F C D I +KC+ C PCK+NG C+ Y+C CP+ F G++CD INAC PC NGG C N N + C CP+GF G KC IN DDC N C N ATC D N+Y C C + G+ CD +C ++NPC + +C + + Y C CS G+ G+ CE N DDC + C+H +QC+D Y C+C PG+ G FCEI P VL + S C +EC N CI Q+ C+C G++G C+ L ++ F K+SY+E+S S + P+ NISL T+ +G++LY+G + L + LYQGH+R+ Y D + P YS+ +ND H + + ++ ++L++ ++ + +L L + L+T +++GG P S + TD F GC+ + IN + DF P SI V PGC N+CK G C K +CE PG + + C C G+ G+ C K+ S C HG S DP D + C + I R Q Y CT T R + + SS CC P K + F+C +G + C C Sbjct: 30 CPSKCTCSGPNVDCHGLGLKTVPKGIPRNAERLDMDKNNITRITKTDFAGLKNLRVLHLEDNQISIIERGAFQDLKQLERMRLNKNKLQVLPELLFQNNAKLTRLDLSENQIQGLPRKSFRGITDVKNLQLDNNQISCIEDGVFRALRELEILTLNNNNITRIPLTSFNHMPKIRTLRLHSNYLYCDCHLTWLSDWLRQRKTI----GQFTFCMSPVHLRGFNVADVQKKDFVCPGTQSE--PPSCNANAVSCPSACTCSNN-VVDCRGKGLTEIPANLPEGIIEIRLEQNSIKAIPAGVFTPYKKLKRIDISKNQISEIAADAFSGLKSLTSLVLYGNKITEIPKGLFDGLVSLQLLLLNANKINCLRVNTFQDLQNLNLLSLYDNKLQTISKGLFAPLQSIQTLHLAQNPFVCDCHLKWLADYLQDNPIETSGARCTSPRRLANKRISQIKSKKFRCTGSEDYRSKFSG---DCFMDLVCPEKCRCEGTIVDCSNQKISRIPSYLPEYTSDLRLNDNEITVLEATGIFKKLPNLRKINLSNNKIKDIREGAFDGAAGVQELMLTGNQLESVHGRMFRGLVGLKTLMLRSNLMSCINNDTFTGLSSVRLLSLYDNRITTITPGAFNTLVSLSTINLLSNPFNCNCHLAWLGKWLRKKRVVSGN---PRCQKPYFLKEIPIQDVAIQDFTCDGNEESSCLPASHCPEQCTCVESV---------------------------VRCGNQGLRTLPKNIPKDVTELYLEGNHLTSVPKEISSFKHL----TLIDLSNNSISVLSNYTFSNVTQLSTLILSYNRLRCIPVHAFNGLKSLRVLTLHGNDISTVPDGSFSDLTSLSHLALGTNPLYCDCNLRWLSEWVKAGYKEPGIARCSGPEDMADRLLLTTPTQKFQCKGPV-DMNIASKCNPCFSNPCKNNGTCNSDPVELYRCTCPFGFKGRDCDIPINACIQAPCENGGTCHTNEANKDGFSCSCPLGFEGEKCEINPDDCEDNDCENFATCVDGINNYICLCPPNYTGELCDEVIDHCVPELNPCLHDSKC-ISTDKGYMCECSPGFVGQNCETNEDDCANHKCRHGAQCIDDIGGYTCVCPPGFSGLFCEISP--PMVL-----------------------LQTSPCDNYECQNKAQCI----VVQQEPVCRCLAGFAGQRCEKLITVNFVGKDSYVELSGSS---VRPQANISLQVATDKDNGILLYKG-DNDPLALELYQGHVRLIY---DTLNSPPTTVYSVETINDGLFHTVELVMLNQSLSLVVDKGLPKSLGK--LQKQPSLNLNTPLYLGGIPPA---SGVPSMRQRTDHIPIGFNGCIHDVRINNELQDFRDLPPVSIGVFPGCKSC-------------------------------NICKH-GSCRSMEKTSVICECFPGWTGSMCDQEIDNTCINHKCIHGECVAANNAYMCKCAEGYTGQYCDKKNDSLSSCRNFKCNHGQCKLSERGDPYCECDPNFTGEHCDKENVCQGEVIREIVRKQHAYTSCTSTVRVPRTECSG---------NCSSGACCVPVKSRKRKYTFQCMDGTSFVEELERYIECGC 1516 HSP 2 Score: 201.06 bits (510), Expect = 3.111e-51 Identity = 147/472 (31.14%), Postives = 232/472 (49.15%), Query Frame = 1 Query: 154 ICPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSD-FLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQLQCINDNIFNKLGMLKYLLLEGNKIFWISE-AISKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCASEDKANLENMC-PSDKKVCSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQIYNFQCNKEFESKYETHDEECVPKVSCPLK--CTCSSDVV 1554 +CP C+CE V+C ++ +P L E T+ L L N I V+ + F KL NL+ + LSNN+I I GAFD + +L L N ++ + +F L L+ L LR N + CIN++ F L ++ L L N+I I+ A + L L + + N NCNCHLAWL +L++K + + + C P+ L IPI ++ +F C N E+ C P+ C ++C C + +V+C + L +P+++P +V EL L+ N++T + + +S +K L I L+ N + + F +TQL+++ L+ N L+ I +AF GL SL L L NDI +P +F +L +L L NPL CDC + W+ +++K +A C P ++ L FQC + + +C P S P K TC+SD V Sbjct: 500 VCPEKCRCEGTIVDCSNQKISRIPSYLPEYTSDLRLNDNEITVLEATGIFKKLPNLRKINLSNNKIKDIREGAFDGAAGVQELMLTGNQLESVHGRMFRGLVGLKTLMLRSNLMSCINNDTFTGLSSVRLLSLYDNRITTITPGAFNTLVSLSTINLLSNPFNCNCHLAWLGKWLRKKRVV----SGNPRCQKPYFLKEIPIQDVAIQDFTC----DGNEESSCLPASH--CPEQCTCV-ESVVRCGNQGLRTLPKNIPKDVTELYLEGNHLTSVPK-EISSFKHLTLIDLSNNSISVLSNYTFSNVTQLSTLILSYNRLRCIPVHAFNGLKSLRVLTLHGNDISTVPDG------------------------SFSDLTSLSHLALGTNPLYCDCNLRWLSEWVKAGYKEPGIARCSGPEDMADRLLLTTPTQKFQCKGPVDMNIAS---KCNPCFSNPCKNNGTCNSDPV 932
BLAST of slit1 vs. Ensembl Xenopus
Match: atad3a (ATPase family, AAA domain containing 3A [Source:Xenbase;Acc:XB-GENE-946129]) HSP 1 Score: 693.345 bits (1788), Expect = 0.000e+0 Identity = 470/1401 (33.55%), Postives = 711/1401 (50.75%), Query Frame = 1 Query: 157 CPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQLQ------------------------CINDNIFNKLGMLKYLLLEGNKIFWISEA-ISKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCASEDKANLENMCPSDKKVCSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQ----------------IYNFQCNKEFESKYETHDEECVPKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLPPIYSIHEAPNLMKLDLSFNQIETLEPDVFTRSSKLREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQ------------------------FHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCD-GNASVYHSVDSCHETNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVLSFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSE----------ETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGIC--KNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCN-DINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNSIYF-QKNSYIEISPPSRGFLMPRGNISLNFMTNSSSGLILYQGSEQTYLVVNLYQGHLRISYNLGDKVVGPEG--YSMGMVNDSRLHALHIEIVGENLTMYLNGRVHCTLKSKYLNSNRYMALDTGIFIGGAPHKILQSAIMKFHIETDSEFIGCMESLLINRKKIDFSKYLPKSINVHPGC 4113 CP C C +V+C +VP + T RL+L N I I K+DF L +L++LQL N+IT+IE GAF +L L +LRLNRN +Q P+ LF KL +LDL +NQ+Q CI D F L L+ L L N I +S A + + L+ R+H N L C+CHLAWL+ +L+Q+ + G ++ C P L I+ + EF C + ++ + + C C C CS++ IV C GK L+ IP +LP + E+ L+ N+I ++ G S YK L++I L+ N + +I AF GL L S+ L N + + K F GLV L L L N I CL D+ + L+LL L +NKL ++ K TF P ++ L+L NP CDC + W+ DY+ T A C PR L + ++ I++ C +++ SK +C ++CP KC C V+CSN L +P+ I T L +++N L + P L K++LS N+I +E F ++ + E+ L SNR++ + ++ FK L+ L+ L L N ISC+ DSF +++ L L NQ F+C+C L W+ +W R V GN P CQ P LK+ PI + + F CD GN +SC + C+ T V CS+ LK +P IPK+ TELYLD N L N +L + LS N I+ L+ + + + L TL+LS+N+L+CI AF L +L++L L NDVS IP AFNDL +L+++A+G NP +CDCN++WL+ + + + GI+ C+ P +M K + + F C+E D I AKC+ C+ PCK++G C+ Y+C CPY F G++CD I+AC PCRN G C K D G + C C GF G C +N+DDC N C N +TC D N+Y+C+C + G+ C+ K +C+ D+NPCQ+ +C +L YKC C+ G+ G+ C+ + DDC+ N C++ ++CVD N Y C+C GY G FCE + P VL S C EC N CI K + C+C PGY G+ C+ L S+ F K+S+I+I P ++ + + NI+L T+ SG++LY+G ++ + V LY+G +R+SY+ G + P YS+ +ND H + + + + L++ ++G ++ + L +++D+ ++IGG P K + + + + + F+GC+ +L IN + DF K L V PGC Sbjct: 37 CPAQCSCTGTTVDCHGLTLRSVPRNIPRNTERLDLNANNITRISKTDFAGLRHLRILQLMENKITTIERGAFQDLKELERLRLNRNNLQVFPELLFLGTPKLYRLDLSENQIQAIPRKAFRGAVDIKNLQLDYNQISCIEDGAFRALRDLEVLTLNNNNITRLSVASFNHMPKLRTFRLHSNNLYCDCHLAWLSDWLRQRPRV---GLYTQ-CMGPIHLRGHNIAEVQKREFVCTGQ-QSFMVHSCSVLH--CPAACTCSNN-IVDCRGKGLTEIPTNLPETITEMRLEQNSIKVIPPGAFSPYKKLRRIDLSNNQISEIAADAFQGLRSLNSLVLYGNKITELPKGLFEGLVLLQLLLLNANKINCLRVDSFQDLHNLNLLSLYDNKLQTIAKGTFAPLRAIQTLHLAQNPFICDCHLKWLADYLHTNPIETSGARCTSPRRLANKRIGQIKSKKFRCSAQEQYFIPGIHHLGCTEDYRSKLSG---DCFADLACPEKCRCEGTTVDCSNQKLTKIPDHIPQYTAELRLNNNEFTVLEATGIFKKLPQLRKINLSNNKITDIEEGAFEGANGVNELLLTSNRMENVRHKMFKGLEGLKTLMLRSNRISCVNNDSFTGLSSVRLLSLYDNQITTVAPGAFDTLHSLSTLNLLANPFNCNCHLAWLGDWLRKKRIVTGN---PRCQKPYFLKEIPIQDVAIQDFTCDDGNED-----NSCSPLSRCPAECTC-----------------LDTVVRCSNKGLKSLPKGIPKEVTELYLDGNMF-PLVPKELSNYKHLTLIDLSNNRISTLSNHSFSNMTQLLTLILSYNRLRCIPLRAFDGLKSLRLLSLHGNDVSAIPEGAFNDLSSLSHLAIGANPLYCDCNLQWLSDWVKSEYKEPGIARCSGPGEMADKLLLTTPSKKFTCNEIPGPCGFPIAGPVDVSILAKCNPCLSNPCKNDGTCNNDPVEFYRCTCPYGFKGQDCDVPIHACISNPCRNSGTCHLKEGDKDG-FWCACAEGFEGENCEVNIDDCEDNDCENNSTCVDGINNYTCRCPPEYTGELCEEKLDFCSQDVNPCQHDSKC-ILTLKGYKCDCTPGYIGEHCDIDYDDCQENKCRNGARCVDAVNGYTCVCPEGYSGLFCE--SAPPMVLP-----------------------RTSPCDNFECQNGAQCIV----KGNEPQCQCLPGYHGNKCEKLVSVNFVNKDSFIQI-PSAK--IRTQANITLQVATDEDSGILLYKG-DKDQVAVELYRGRVRVSYDTG---INPASAIYSVETINDGNFHVVELVNLDQTLSLVVDGGTPKSITN--LPKQSLISIDSTLYIGGMPMKNNIAGLRQSSSQNGTNFLGCIRNLYINNELQDFRK-LNLQAGVLPGC 1359 HSP 2 Score: 58.5362 bits (140), Expect = 1.103e-7 Identity = 43/115 (37.39%), Postives = 58/115 (50.43%), Query Frame = 1 Query: 2917 GICKNIDNYGNYECVCPVGFRGPKCTINVDD-CVGNLCVNGATCNDLNN-SYSCKCQSGFRGKYCDIKFLYCNDINPCQN----SGQCQLLPNNDYKCVCSKGWEGKQCERNTDD 3243 G C G + C C G+ G C I +D C+GN CV+G TC +N+ SYSCKC GF G CD + NPCQ+ G+C+L C C+ G+ G C++ D Sbjct: 1370 GTCLATSQAG-FTCECEGGWTGQLCDIQSNDPCLGNKCVHG-TCLPINSFSYSCKCLQGFVGVLCDEPE---DQPNPCQSIKCKHGKCRLSGLGKAYCECNSGYTGDSCDKAFTD 1479
BLAST of slit1 vs. Ensembl Xenopus
Match: atad3a (ATPase family, AAA domain containing 3A [Source:Xenbase;Acc:XB-GENE-946129]) HSP 1 Score: 690.649 bits (1781), Expect = 0.000e+0 Identity = 468/1391 (33.64%), Postives = 708/1391 (50.90%), Query Frame = 1 Query: 157 CPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQLQ------------------------CINDNIFNKLGMLKYLLLEGNKIFWISEA-ISKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCASEDKANLENMCPSDKKVCSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQIYNFQCNKE-------FESKYETHDEECVPKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLPPIYSIHEAPNLMKLDLSFNQIETLEPDVFTRSSKLREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQ------------------------FHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCD-GNASVYHSVDSCHETNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVLSFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGIC--KNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGF---------RGKYCDIKFLYCN-DINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNSIYF-QKNSYIEISPPSRGFLMPRGNISLNFMTNSSSGLILYQGSEQTYLVVNLYQGHLRISYNLGDKVVGPEG--YSMGMVNDSRLHALHIEIVGENLTMYLNGRVHCTLKSKYLNSNRYMALDTGIFIGGAPHKILQSAIMKFHIETDSEFIGCMESLLINRKKIDFSKYLPKSINVHPGC 4113 CP C C +V+C +VP + T RL+L N I I K+DF L +L++LQL N+IT+IE GAF +L L +LRLNRN +Q P+ LF KL +LDL +NQ+Q CI D F L L+ L L N I +S A + + L+ R+H N L C+CHLAWL+ +L+Q+ + G ++ C P L I+ + EF C + ++ + + C C C CS++ IV C GK L+ IP +LP + E+ L+ N+I ++ G S YK L++I L+ N + +I AF GL L S+ L N + + K F GLV L L L N I CL D+ + L+LL L +NKL ++ K TF P ++ L+L NP CDC + W+ DY+ T A C PR L + ++ F+C+ + E +C ++CP KC C V+CSN L +P+ I T L +++N L + P L K++LS N+I +E F ++ + E+ L SNR++ + ++ FK L+ L+ L L N ISC+ DSF +++ L L NQ F+C+C L W+ +W R V GN P CQ P LK+ PI + + F CD GN +SC + C+ T V CS+ LK +P IPK+ TELYLD N L N +L + LS N I+ L+ + + + L TL+LS+N+L+CI AF L +L++L L NDVS IP AFNDL +L+++A+G NP +CDCN++WL+ + + + GI+ C+ P +M K + + F C+ D I AKC+ C+ PCK++G C+ Y+C CPY F G++CD I+AC PCRN G C K D G + C C GF G C +N+DDC N C N +TC D N+Y+C+C + +G+ C+ K +C+ D+NPCQ+ +C +L YKC C+ G+ G+ C+ + DDC+ N C++ ++CVD N Y C+C GY G FCE + P VL S C EC N CI K + C+C PGY G+ C+ L S+ F K+S+I+I P ++ + + NI+L T+ SG++LY+G ++ + V LY+G +R+SY+ G + P YS+ +ND H + + + + L++ ++G ++ + L +++D+ ++IGG P K + + + + + F+GC+ +L IN + DF K L V PGC Sbjct: 28 CPAQCSCTGTTVDCHGLTLRSVPRNIPRNTERLDLNANNITRISKTDFAGLRHLRILQLMENKITTIERGAFQDLKELERLRLNRNNLQVFPELLFLGTPKLYRLDLSENQIQAIPRKAFRGAVDIKNLQLDYNQISCIEDGAFRALRDLEVLTLNNNNITRLSVASFNHMPKLRTFRLHSNNLYCDCHLAWLSDWLRQRPRV---GLYTQ-CMGPIHLRGHNIAEVQKREFVCTGQ-QSFMVHSCSVLH--CPAACTCSNN-IVDCRGKGLTEIPTNLPETITEMRLEQNSIKVIPPGAFSPYKKLRRIDLSNNQISEIAADAFQGLRSLNSLVLYGNKITELPKGLFEGLVLLQLLLLNANKINCLRVDSFQDLHNLNLLSLYDNKLQTIAKGTFAPLRAIQTLHLAQNPFICDCHLKWLADYLHTNPIETSGARCTSPRRLANKRIGQIKSKKFRCSAQEQYFIPGTEDYRSKLSGDCFADLACPEKCRCEGTTVDCSNQKLTKIPDHIPQYTAELRLNNNEFTVLEATGIFKKLPQLRKINLSNNKITDIEEGAFEGANGVNELLLTSNRMENVRHKMFKGLEGLKTLMLRSNRISCVNNDSFTGLSSVRLLSLYDNQITTVAPGAFDTLHSLSTLNLLANPFNCNCHLAWLGDWLRKKRIVTGN---PRCQKPYFLKEIPIQDVAIQDFTCDDGNED-----NSCSPLSRCPAECTC-----------------LDTVVRCSNKGLKSLPKGIPKEVTELYLDGNMF-PLVPKELSNYKHLTLIDLSNNRISTLSNHSFSNMTQLLTLILSYNRLRCIPLRAFDGLKSLRLLSLHGNDVSAIPEGAFNDLSSLSHLAIGANPLYCDCNLQWLSDWVKSEYKEPGIARCSGPGEMADKLLLTTPSKKFTCNGPV-DVSILAKCNPCLSNPCKNDGTCNNDPVEFYRCTCPYGFKGQDCDVPIHACISNPCRNSGTCHLKEGDKDG-FWCACAEGFEGENCEVNIDDCEDNDCENNSTCVDGINNYTCRCPPEYTAAKLNEVEKGELCEEKLDFCSQDVNPCQHDSKC-ILTLKGYKCDCTPGYIGEHCDIDYDDCQENKCRNGARCVDAVNGYTCVCPEGYSGLFCE--SAPPMVLP-----------------------RTSPCDNFECQNGAQCIV----KGNEPQCQCLPGYHGNKCEKLVSVNFVNKDSFIQI-PSAK--IRTQANITLQVATDEDSGILLYKG-DKDQVAVELYRGRVRVSYDTG---INPASAIYSVETINDGNFHVVELVNLDQTLSLVVDGGTPKSITN--LPKQSLISIDSTLYIGGMPMKNNIAGLRQSSSQNGTNFLGCIRNLYINNELQDFRK-LNLQAGVLPGC 1342 HSP 2 Score: 57.7658 bits (138), Expect = 2.284e-7 Identity = 42/111 (37.84%), Postives = 57/111 (51.35%), Query Frame = 1 Query: 2917 GICKNIDNYGNYECVCPVGFRGPKCTINVDD-CVGNLCVNGATCNDLNN-SYSCKCQSGFRGKYCDIKFLYCNDINPCQN----SGQCQLLPNNDYKCVCSKGWEGKQCER 3231 G C G + C C G+ G C I +D C+GN CV+G TC +N+ SYSCKC GF G CD + NPCQ+ G+C+L C C+ G+ G C++ Sbjct: 1353 GTCLATSQAG-FTCECEGGWTGQLCDIQSNDPCLGNKCVHG-TCLPINSFSYSCKCLQGFVGVLCDEPE---DQPNPCQSIKCKHGKCRLSGLGKAYCECNSGYTGDSCDK 1458
BLAST of slit1 vs. Ensembl Xenopus
Match: atad3a (ATPase family, AAA domain containing 3A [Source:Xenbase;Acc:XB-GENE-946129]) HSP 1 Score: 690.263 bits (1780), Expect = 0.000e+0 Identity = 470/1396 (33.67%), Postives = 712/1396 (51.00%), Query Frame = 1 Query: 157 CPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQLQ------------------------CINDNIFNKLGMLKYLLLEGNKIFWISEA-ISKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCASEDKANLENMCPSDKKVCSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQIYNFQCN--KEFESKYETHDEECVPKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLPPIYSIHEAPNLMKLDLSFNQIETLEPDVFTRSSKLREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQ------------------------FHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCD-GNASVYHSVDSCHETNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVLSFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSE----------ETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGIC--KNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGF---------RGKYCDIKFLYCN-DINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNSIYF-QKNSYIEISPPSRGFLMPRGNISLNFMTNSSSGLILYQGSEQTYLVVNLYQGHLRISYNLGDKVVGPEG--YSMGMVNDSRLHALHIEIVGENLTMYLNGRVHCTLKSKYLNSNRYMALDTGIFIGGAPHKILQSAIMKFHIETDSEFIGCMESLLINRKKIDFSKYLPKSINVHPGC 4113 CP C C +V+C +VP + T RL+L N I I K+DF L +L++LQL N+IT+IE GAF +L L +LRLNRN +Q P+ LF KL +LDL +NQ+Q CI D F L L+ L L N I +S A + + L+ R+H N L C+CHLAWL+ +L+Q+ + G ++ C P L I+ + EF C + ++ + + C C C CS++ IV C GK L+ IP +LP + E+ L+ N+I ++ G S YK L++I L+ N + +I AF GL L S+ L N + + K F GLV L L L N I CL D+ + L+LL L +NKL ++ K TF P ++ L+L NP CDC + W+ DY+ T A C PR L + ++ F+C+ +++ SK +C ++CP KC C V+CSN L +P+ I T L +++N L + P L K++LS N+I +E F ++ + E+ L SNR++ + ++ FK L+ L+ L L N ISC+ DSF +++ L L NQ F+C+C L W+ +W R V GN P CQ P LK+ PI + + F CD GN +SC + C+ T V CS+ LK +P IPK+ TELYLD N L N +L + LS N I+ L+ + + + L TL+LS+N+L+CI AF L +L++L L NDVS IP AFNDL +L+++A+G NP +CDCN++WL+ + + + GI+ C+ P +M K + + F C+E D I AKC+ C+ PCK++G C+ Y+C CPY F G++CD I+AC PCRN G C K D G + C C GF G C +N+DDC N C N +TC D N+Y+C+C + +G+ C+ K +C+ D+NPCQ+ +C +L YKC C+ G+ G+ C+ + DDC+ N C++ ++CVD N Y C+C GY G FCE + P VL S C EC N CI K + C+C PGY G+ C+ L S+ F K+S+I+I P ++ + + NI+L T+ SG++LY+G ++ + V LY+G +R+SY+ G + P YS+ +ND H + + + + L++ ++G ++ + L +++D+ ++IGG P K + + + + + F+GC+ +L IN + DF K L V PGC Sbjct: 37 CPAQCSCTGTTVDCHGLTLRSVPRNIPRNTERLDLNANNITRISKTDFAGLRHLRILQLMENKITTIERGAFQDLKELERLRLNRNNLQVFPELLFLGTPKLYRLDLSENQIQAIPRKAFRGAVDIKNLQLDYNQISCIEDGAFRALRDLEVLTLNNNNITRLSVASFNHMPKLRTFRLHSNNLYCDCHLAWLSDWLRQRPRV---GLYTQ-CMGPIHLRGHNIAEVQKREFVCTGQ-QSFMVHSCSVLH--CPAACTCSNN-IVDCRGKGLTEIPTNLPETITEMRLEQNSIKVIPPGAFSPYKKLRRIDLSNNQISEIAADAFQGLRSLNSLVLYGNKITELPKGLFEGLVLLQLLLLNANKINCLRVDSFQDLHNLNLLSLYDNKLQTIAKGTFAPLRAIQTLHLAQNPFICDCHLKWLADYLHTNPIETSGARCTSPRRLANKRIGQIKSKKFRCSGTEDYRSKLSG---DCFADLACPEKCRCEGTTVDCSNQKLTKIPDHIPQYTAELRLNNNEFTVLEATGIFKKLPQLRKINLSNNKITDIEEGAFEGANGVNELLLTSNRMENVRHKMFKGLEGLKTLMLRSNRISCVNNDSFTGLSSVRLLSLYDNQITTVAPGAFDTLHSLSTLNLLANPFNCNCHLAWLGDWLRKKRIVTGN---PRCQKPYFLKEIPIQDVAIQDFTCDDGNED-----NSCSPLSRCPAECTC-----------------LDTVVRCSNKGLKSLPKGIPKEVTELYLDGNMF-PLVPKELSNYKHLTLIDLSNNRISTLSNHSFSNMTQLLTLILSYNRLRCIPLRAFDGLKSLRLLSLHGNDVSAIPEGAFNDLSSLSHLAIGANPLYCDCNLQWLSDWVKSEYKEPGIARCSGPGEMADKLLLTTPSKKFTCNEIPGPCGFPIAGPVDVSILAKCNPCLSNPCKNDGTCNNDPVEFYRCTCPYGFKGQDCDVPIHACISNPCRNSGTCHLKEGDKDG-FWCACAEGFEGENCEVNIDDCEDNDCENNSTCVDGINNYTCRCPPEYTAAKLNEVEKGELCEEKLDFCSQDVNPCQHDSKC-ILTLKGYKCDCTPGYIGEHCDIDYDDCQENKCRNGARCVDAVNGYTCVCPEGYSGLFCE--SAPPMVLP-----------------------RTSPCDNFECQNGAQCIV----KGNEPQCQCLPGYHGNKCEKLVSVNFVNKDSFIQI-PSAK--IRTQANITLQVATDEDSGILLYKG-DKDQVAVELYRGRVRVSYDTG---INPASAIYSVETINDGNFHVVELVNLDQTLSLVVDGGTPKSITN--LPKQSLISIDSTLYIGGMPMKNNIAGLRQSSSQNGTNFLGCIRNLYINNELQDFRK-LNLQAGVLPGC 1354 HSP 2 Score: 57.3806 bits (137), Expect = 2.406e-7 Identity = 42/111 (37.84%), Postives = 57/111 (51.35%), Query Frame = 1 Query: 2917 GICKNIDNYGNYECVCPVGFRGPKCTINVDD-CVGNLCVNGATCNDLNN-SYSCKCQSGFRGKYCDIKFLYCNDINPCQN----SGQCQLLPNNDYKCVCSKGWEGKQCER 3231 G C G + C C G+ G C I +D C+GN CV+G TC +N+ SYSCKC GF G CD + NPCQ+ G+C+L C C+ G+ G C++ Sbjct: 1365 GTCLATSQAG-FTCECEGGWTGQLCDIQSNDPCLGNKCVHG-TCLPINSFSYSCKCLQGFVGVLCDEPE---DQPNPCQSIKCKHGKCRLSGLGKAYCECNSGYTGDSCDK 1470
BLAST of slit1 vs. Ensembl Xenopus
Match: atad3a (ATPase family, AAA domain containing 3A [Source:Xenbase;Acc:XB-GENE-946129]) HSP 1 Score: 689.108 bits (1777), Expect = 0.000e+0 Identity = 469/1400 (33.50%), Postives = 711/1400 (50.79%), Query Frame = 1 Query: 157 CPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQLQ------------------------CINDNIFNKLGMLKYLLLEGNKIFWISEA-ISKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCASEDKANLENMCPSDKKVCSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQ----------------IYNFQCNKEFESKYETHDEECVPKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLPPIYSIHEAPNLMKLDLSFNQIETLEPDVFTRSSKLREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQ------------------------FHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCD-GNASVYHSVDSCHETNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVLSFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGIC--KNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGF---------RGKYCDIKFLYCN-DINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNSIYF-QKNSYIEISPPSRGFLMPRGNISLNFMTNSSSGLILYQGSEQTYLVVNLYQGHLRISYNLGDKVVGPEG--YSMGMVNDSRLHALHIEIVGENLTMYLNGRVHCTLKSKYLNSNRYMALDTGIFIGGAPHKILQSAIMKFHIETDSEFIGCMESLLINRKKIDFSKYLPKSINVHPGC 4113 CP C C +V+C +VP + T RL+L N I I K+DF L +L++LQL N+IT+IE GAF +L L +LRLNRN +Q P+ LF KL +LDL +NQ+Q CI D F L L+ L L N I +S A + + L+ R+H N L C+CHLAWL+ +L+Q+ + G ++ C P L I+ + EF C + ++ + + C C C CS++ IV C GK L+ IP +LP + E+ L+ N+I ++ G S YK L++I L+ N + +I AF GL L S+ L N + + K F GLV L L L N I CL D+ + L+LL L +NKL ++ K TF P ++ L+L NP CDC + W+ DY+ T A C PR L + ++ I++ C +++ SK +C ++CP KC C V+CSN L +P+ I T L +++N L + P L K++LS N+I +E F ++ + E+ L SNR++ + ++ FK L+ L+ L L N ISC+ DSF +++ L L NQ F+C+C L W+ +W R V GN P CQ P LK+ PI + + F CD GN +SC + C+ T V CS+ LK +P IPK+ TELYLD N L N +L + LS N I+ L+ + + + L TL+LS+N+L+CI AF L +L++L L NDVS IP AFNDL +L+++A+G NP +CDCN++WL+ + + + GI+ C+ P +M K + + F C+ D I AKC+ C+ PCK++G C+ Y+C CPY F G++CD I+AC PCRN G C K D G + C C GF G C +N+DDC N C N +TC D N+Y+C+C + +G+ C+ K +C+ D+NPCQ+ +C +L YKC C+ G+ G+ C+ + DDC+ N C++ ++CVD N Y C+C GY G FCE + P VL S C EC N CI K + C+C PGY G+ C+ L S+ F K+S+I+I P ++ + + NI+L T+ SG++LY+G ++ + V LY+G +R+SY+ G + P YS+ +ND H + + + + L++ ++G ++ + L +++D+ ++IGG P K + + + + + F+GC+ +L IN + DF K L V PGC Sbjct: 37 CPAQCSCTGTTVDCHGLTLRSVPRNIPRNTERLDLNANNITRISKTDFAGLRHLRILQLMENKITTIERGAFQDLKELERLRLNRNNLQVFPELLFLGTPKLYRLDLSENQIQAIPRKAFRGAVDIKNLQLDYNQISCIEDGAFRALRDLEVLTLNNNNITRLSVASFNHMPKLRTFRLHSNNLYCDCHLAWLSDWLRQRPRV---GLYTQ-CMGPIHLRGHNIAEVQKREFVCTGQ-QSFMVHSCSVLH--CPAACTCSNN-IVDCRGKGLTEIPTNLPETITEMRLEQNSIKVIPPGAFSPYKKLRRIDLSNNQISEIAADAFQGLRSLNSLVLYGNKITELPKGLFEGLVLLQLLLLNANKINCLRVDSFQDLHNLNLLSLYDNKLQTIAKGTFAPLRAIQTLHLAQNPFICDCHLKWLADYLHTNPIETSGARCTSPRRLANKRIGQIKSKKFRCSAQEQYFIPGIHHLGCTEDYRSKLSG---DCFADLACPEKCRCEGTTVDCSNQKLTKIPDHIPQYTAELRLNNNEFTVLEATGIFKKLPQLRKINLSNNKITDIEEGAFEGANGVNELLLTSNRMENVRHKMFKGLEGLKTLMLRSNRISCVNNDSFTGLSSVRLLSLYDNQITTVAPGAFDTLHSLSTLNLLANPFNCNCHLAWLGDWLRKKRIVTGN---PRCQKPYFLKEIPIQDVAIQDFTCDDGNED-----NSCSPLSRCPAECTC-----------------LDTVVRCSNKGLKSLPKGIPKEVTELYLDGNMF-PLVPKELSNYKHLTLIDLSNNRISTLSNHSFSNMTQLLTLILSYNRLRCIPLRAFDGLKSLRLLSLHGNDVSAIPEGAFNDLSSLSHLAIGANPLYCDCNLQWLSDWVKSEYKEPGIARCSGPGEMADKLLLTTPSKKFTCNGPV-DVSILAKCNPCLSNPCKNDGTCNNDPVEFYRCTCPYGFKGQDCDVPIHACISNPCRNSGTCHLKEGDKDG-FWCACAEGFEGENCEVNIDDCEDNDCENNSTCVDGINNYTCRCPPEYTAAKLNEVEKGELCEEKLDFCSQDVNPCQHDSKC-ILTLKGYKCDCTPGYIGEHCDIDYDDCQENKCRNGARCVDAVNGYTCVCPEGYSGLFCE--SAPPMVLP-----------------------RTSPCDNFECQNGAQCIV----KGNEPQCQCLPGYHGNKCEKLVSVNFVNKDSFIQI-PSAK--IRTQANITLQVATDEDSGILLYKG-DKDQVAVELYRGRVRVSYDTG---INPASAIYSVETINDGNFHVVELVNLDQTLSLVVDGGTPKSITN--LPKQSLISIDSTLYIGGMPMKNNIAGLRQSSSQNGTNFLGCIRNLYINNELQDFRK-LNLQAGVLPGC 1357 HSP 2 Score: 58.5362 bits (140), Expect = 1.057e-7 Identity = 43/115 (37.39%), Postives = 58/115 (50.43%), Query Frame = 1 Query: 2917 GICKNIDNYGNYECVCPVGFRGPKCTINVDD-CVGNLCVNGATCNDLNN-SYSCKCQSGFRGKYCDIKFLYCNDINPCQN----SGQCQLLPNNDYKCVCSKGWEGKQCERNTDD 3243 G C G + C C G+ G C I +D C+GN CV+G TC +N+ SYSCKC GF G CD + NPCQ+ G+C+L C C+ G+ G C++ D Sbjct: 1368 GTCLATSQAG-FTCECEGGWTGQLCDIQSNDPCLGNKCVHG-TCLPINSFSYSCKCLQGFVGVLCDEPE---DQPNPCQSIKCKHGKCRLSGLGKAYCECNSGYTGDSCDKAFTD 1477
BLAST of slit1 vs. Ensembl Mouse
Match: Slit3 (slit guidance ligand 3 [Source:MGI Symbol;Acc:MGI:1315202]) HSP 1 Score: 729.169 bits (1881), Expect = 0.000e+0 Identity = 468/1381 (33.89%), Postives = 709/1381 (51.34%), Query Frame = 1 Query: 148 SEICPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQLQ------------------------CINDNIFNKLGMLKYLLLEGNKIFWI-SEAISKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCASEDKANLENMCPSDKKVCSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQIYNFQC--NKEFESKYETHDEECVPKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLPPIYSIHEAPNLMKLDLS------------------------FNQIETLEPDVFTRSSKLREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQFHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCDGNASVYHSVDSCHETNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNE--TSFKNLLNLKTLV-LSYNGITELNKNVLTPLKSLETLVLSFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGN-YECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYC-NDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNSIYF-QKNSYIEISPPSRGFLMPRGNISLNFMTNSSSGLILYQGSEQTYLVVNLYQGHLRISYNLGDKVVGPEG--YSMGMVNDSRLHALHIEIVGENLTMYLNGRVHCTLKSKYLNSNRYMALDTGIFIGGAPHKILQSAIMKFHIETDSEFIGCMESLLINRKKIDFSKYLPKSINVHPGC 4113 + CP C C SV+C VP + RL+L N I I K DF L NL+VL L +N+++ IE GAF +L L +LRLN+N +Q LP+ LF KL +LDL +NQ+Q CI D F L L+ L L N I I + + + ++ LR+H N L C+CHLAWL+ +L+Q+ I LC P L ++ + E+ C C ++ C C+CS++ IV C GK L+ IP +LP +VE+ L+ N+I + G ++YK LK+I +++N + DI P AF GL LTS+ L N + I K F GLVSL L L N I CL +T + + L+LL L +NKL ++ K F P +++ L+L NP CDC + W+ DY++D T A C PR L + ++ F+C ++++ +++ + EC + CP KC C +V+CSN L +P + T L ++ N++ L + PNL K++LS NQ+ET+ +F S L+ + L SN + C++N+TF L + +L L +N I+ I +F ++L + L N F+C+C + W+ W R V GN P CQ P LK+ PI + + F CDGN SC + C+ T V CS+ L +P +PKD TELYL+ NHL ++ + ++F+ L TL+ LS N I+ L + + + L TL+LS+N+L+CI AF L +L+VL L ND+S++P +FNDL +L+++ALG NP HCDC+++WL+++ + + GI+ C+SPE M + + + F C D I AKC++C+ PCK+NG CS Y+C CPY + GK+C IN C PC +GG C +N + + C CP+GF G +C IN DDC N C N ATC D N+Y+C C + G+ CD YC ++N CQ+ +C L + ++C C G+ GK CE N DDC + C+H +QCVD N Y C+C G+ G FCE P P VL + S C +EC N CI Q+ C+C PG++G C+ L ++ F K+SY+E++ + P+ NISL T+ +G++LY+G + L + LYQGH+R+ Y D + P YS+ VND + H++ + ++ + L + ++ +L L + ++ +++GG P SA+ + F GC+ + IN + DF P+S+ V PGC Sbjct: 31 AAACPTKCTCSAASVDCHGLGLRAVPRGIPRNAERLDLDRNNITRITKMDFAGLKNLRVLHLEDNQVSIIERGAFQDLKQLERLRLNKNKLQVLPELLFQSTPKLTRLDLSENQIQGIPRKAFRGVTGVKNLQLDNNHISCIEDGAFRALRDLEILTLNNNNISRILVTSFNHMPKIRTLRLHSNHLYCDCHLAWLSDWLRQRRTI----GQFTLCMAPVHLRGFSVADVQKKEYVCPGPHSE--APACNANSLSCPSACSCSNN-IVDCRGKGLTEIPANLPEGIVEIRLEQNSIKSIPAGAFTQYKKLKRIDISKNQISDIAPDAFQGLKSLTSLVLYGNKITEIPKGLFDGLVSLQLLLLNANKINCLRVNTFQDLQNLNLLSLYDNKLQTISKGLFVPLQSIQTLHLAQNPFVCDCHLKWLADYLQDNPIETSGARCSSPRRLANKRISQIKSKKFRCSGSEDYRNRFSS---ECFMDLVCPEKCRCEGTIVDCSNQKLARIPSHLPEYTTDLRLNDNDISVLEATGIFKKLPNLRKINLSNNRIKEVREGAFDGAAGVQELMLTGNQLETMHGRMFRGLSSLKTLMLRSNLISCVSNDTFAGLSSVRLLSLYDNRITTITPGAFTTLVSLSTINLLSNPFNCNCHMAWLGRWLRKRRIVSGN---PRCQKPFFLKEIPIQDVAIQDFTCDGNEE-----SSCQLSPRCPEQCTC-----------------VETVVRCSNRGLHALPKGMPKDVTELYLEGNHLTAVPKELSAFRQL----TLIDLSNNSISMLTNHTFSNMSHLSTLILSYNRLRCIPVHAFNGLRSLRVLTLHGNDISSVPEGSFNDLTSLSHLALGTNPLHCDCSLRWLSEWVKAGYKEPGIARCSSPESMADRLLLTTPTHRFQCKGPV-DINIVAKCNACLSSPCKNNGTCSQDPVEQYRCTCPYSYKGKDCTVPINTCVQNPCEHGGTCHLSENLRDGFSCSCPLGFEGQRCEINPDDCEDNDCENSATCVDGINNYACLCPPNYTGELCDEVIDYCVPEMNLCQHEAKCISL-DKGFRCECVPGYSGKLCETNNDDCVAHKCRHGAQCVDEVNGYTCICPQGFSGLFCEHPP--PMVL-----------------------LQTSPCDQYECQNGAQCI----VVQQEPTCRCPPGFAGPRCEKLITVNFVGKDSYVELASAK---VRPQANISLQVATDKDNGILLYKG-DNDPLALELYQGHVRLVY---DSLSSPPTTVYSVETVNDGQFHSVELVMLNQTLNLVVDKGAPKSLGK--LQKQPAVGSNSPLYLGGIPTSTGLSALRQGADRPLGGFHGCIHEVRINNELQDFKALPPQSLGVSPGC 1332 HSP 2 Score: 186.422 bits (472), Expect = 9.019e-47 Identity = 128/479 (26.72%), Postives = 213/479 (44.47%), Query Frame = 1 Query: 1507 PKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLP-----------------------------------------------PIYSIHEAPNLMKLDLSFNQIETLEPDVFTRSSKLREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQFHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCDGNASVYHSVDSCHETNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVL------------------------SFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICS 2730 P +CP KCTCS+ V+C GL ++P I + + L + NN+ ++ P P L +LDLS NQI+ + F + ++ + L++N + CI + F+ L++LEIL L+ N IS I+ SF + ++ L L+ N +CDC L W+S+W R T+ + C +P+ L+ + + K ++C G S + ++ + S + CS N VDC L EIP +P+ E+ L++N ++S+ +F LK + +S N I+++ + LKSL +LVL + NK+ C+ + F++L NL +L L N + TI F L+++ + L QNPF CDC++KWL + D ++ + C+SP ++ K I K F CS Sbjct: 30 PAAACPTKCTCSAASVDCHGLGLRAVPRGIPRNAERLDLDRNNITRITKMDFAGLKNLRVLHLEDNQVSIIERGAFQDLKQLERLRLNKNKLQVLPELLFQSTPKLTRLDLSENQIQGIPRKAFRGVTGVKNLQLDNNHISCIEDGAFRALRDLEILTLNNNNISRILVTSFNHMPKIRTLRLHSNHLYCDCHLAWLSDWLRQRRTI---GQFTLCMAPVHLRGFSVADVQKKEYVCPGPHSEAPACNA--NSLSCPSACSCSN-----------------NIVDCRGKGLTEIPANLPEGIVEIRLEQNSIKSIPAGAFTQYKKLKRIDISKNQISDIAPDAFQGLKSLTSLVLYGNKITEIPKGLFDGLVSLQLLLLNANKINCLRVNTFQDLQNLNLLSLYDNKLQTISKGLFVPLQSIQTLHLAQNPFVCDCHLKWLADYLQDNPIETSGARCSSPRRLANKRISQIKSKKFRCS 486 HSP 3 Score: 56.225 bits (134), Expect = 4.913e-7 Identity = 39/115 (33.91%), Postives = 59/115 (51.30%), Query Frame = 1 Query: 2905 CRNGGICKNIDNYGNYECVCPVGFRGPKCTINV-DDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCND-INPCQ----NSGQCQLLPNNDYKCVCSKGWEGKQCER 3231 CR+G +C++++ + C C G+ GP C D C+G+ C +G TC +SY CKC G+ G CD K ND + C + GQC + + C+C G+ G CE+ Sbjct: 1338 CRHG-LCRSVEK-DSVVCECHPGWTGPLCDQEARDPCLGHSCRHG-TCMATGDSYVCKCAEGYGGALCDQK----NDSASACSAFKCHHGQCHISDRGEPYCLCQPGFSGHHCEQ 1445 HSP 4 Score: 52.7582 bits (125), Expect = 5.665e-6 Identity = 39/141 (27.66%), Postives = 60/141 (42.55%), Query Frame = 1 Query: 3154 GQCQLLPNNDYKCVCSKGWEGKQCERNT-DDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLN 3573 G C+ + + C C GW G C++ D C +SC+H + C+ + Y C C GY G C+ + D S S C+ +C ++G C D+ + YC C+PG+SGH C+ N Sbjct: 1341 GLCRSVEKDSVVCECHPGWTGPLCDQEARDPCLGHSCRHGT-CMATGDSYVCKCAEGYGGALCD-------------QKND----------------SASACSAFKC-HHGQCHISDRG---EPYCLCQPGFSGHHCEQEN 1447
BLAST of slit1 vs. Ensembl Mouse
Match: Slit2 (slit guidance ligand 2 [Source:MGI Symbol;Acc:MGI:1315205]) HSP 1 Score: 702.59 bits (1812), Expect = 0.000e+0 Identity = 486/1483 (32.77%), Postives = 734/1483 (49.49%), Query Frame = 1 Query: 151 EICPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQLQ------------------------CINDNIFNKLGMLKYLLLEGNKIFWISEA-ISKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCASEDKANLENMCPSDKKV-CSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQIYNFQCN--KEFESKYETHDEECVPKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLPPIYSIHEAPNLMKLDLSFNQIETLEPDVFTRSSKLREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQ------------------------FHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCD-GNASVYHSVDSCHETNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVLSFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGIC--KNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYC-NDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNSIYF-QKNSYIEISPPSRGFLMPRGNISLNFMTNSSSGLILYQGSEQTYLVVNLYQGHLRISYNLGDKVVGPEGYSMGMVNDSRLHALHIEIVGENLTMYLNGRVHCTLKSKYLNSNRYMALDTGIFIGGAPHKILQSAIMKFHIETDSEFIGCMESLLINRKKIDFSKYLPKSINVHPGCGKLISTVVASDLKIPSIMXXXXXXXXXXXXXXXNNVCKRPGICKNQGVCEVLPGSKKKFRCICKNGFVGRKCKKRKSMCHGTFTESHIVDPDSQGTCKSIERFQY 4428 + CP C C +V+C +VP + T RL+L GN I I K DF L +L+VLQL NRI++IE GAF +L L +LRLNRN +Q P+ LF KL +LDL +NQ+Q CI D F L L+ L L N I +S A + + L+ R+H N L C+CHLAWL+ +L+Q+ + G ++ C P L ++ + EF C+ E++ + M PS + C C CS++ IV C GK L+ IP +LP + E+ L+ N+I ++ G S YK L+++ L+ N + ++ P AF GL L S+ L N + + K+ F GL SL L L N I CL D + L+LL L +NKL ++ K TF ++ ++L NP CDC + W+ DY+ T A C PR L + ++ F+C+ +++ SK +C ++CP KC C V+CSN L +P+ I T L +++N L + P L K++ S N+I +E F +S + EI L SNRL+ + ++ FK L+ L+ L L N ISC+ DSF +++ L L NQ F+C+C L W+ EW R V GN P CQ P LK+ PI + + F CD GN +SC + + C+ T V CS+ LK +P IPKD TELYLD N +L N +L + LS N I+ L+ + + L TL+LS+N+L+CI F L +L++L L ND+S +P AFNDL L+++A+G NP +CDCN++WL+ + + + GI+ CA P +M K + + F C D I AKC+ C+ PCK++G C+ Y+C CPY F G++CD I+AC PC++GG C K +N G + C C GF G C +N+DDC N C N +TC D N+Y+C C + G+ C+ K +C D+NPCQ+ +C L P +KC C+ G+ G+ C+ + DDC+ N CK+ + C D N Y C+C GY G FCE P VL S C +C N CI + + C+C PGY G C+ L S+ F K SY++I P ++ + P+ NI+L T+ SG++LY+G ++ ++ V LY+G +R SY+ G YS+ +ND H + + + +L++ ++G + + L+ + D+ +++GG P K +++ + + + F GC+ +L IN + DF K +P + PGC V A G+C+ P S+ F C C+ G++G C +R + + + + GTC I F Y Sbjct: 26 QACPAQCSCSGSTVDCHGLALRSVPRNIPRNTERLDLNGNNITRITKIDFAGLRHLRVLQLMENRISTIERGAFQDLKELERLRLNRNNLQLFPELLFLGTAKLYRLDLSENQIQAIPRKAFRGAVDIKNLQLDYNQISCIEDGAFRALRDLEVLTLNNNNITRLSVASFNHMPKLRTFRLHSNNLYCDCHLAWLSDWLRQRPRV---GLYTQ-CMGPSHLRGHNVAEVQKREFVCSDEEEGHQSFMAPSCSVLHCPAACTCSNN-IVDCRGKGLTEIPTNLPETITEIRLEQNSIRVIPPGAFSPYKKLRRLDLSNNQISELAPDAFQGLRSLNSLVLYGNKITELPKSLFEGLFSLQLLLLNANKINCLRVDAFQDLHNLNLLSLYDNKLQTVAKGTFSALRAIQTMHLAQNPFICDCHLKWLADYLHTNPIETSGARCTSPRRLANKRIGQIKSKKFRCSGTEDYRSKLSG---DCFADLACPEKCRCEGTTVDCSNQRLNKIPDHIPQYTAELRLNNNEFTVLEATGIFKKLPQLRKINFSNNKITDIEEGAFEGASGVNEILLTSNRLENVQHKMFKGLESLKTLMLRSNRISCVGNDSFIGLGSVRLLSLYDNQITTVAPGAFDSLHSLSTLNLLANPFNCNCHLAWLGEWLRRKRIVTGN---PRCQKPYFLKEIPIQDVAIQDFTCDDGNDD-----NSCSPLSRCPSECTC-----------------LDTVVRCSNKGLKVLPKGIPKDVTELYLDGNQF-TLVPKELSNYKHLTLIDLSNNRISTLSNQSFSNMTQLLTLILSYNRLRCIPPRTFDGLKSLRLLSLHGNDISVVPEGAFNDLSALSHLAIGANPLYCDCNMQWLSDWVKSEYKEPGIARCAGPGEMADKLLLTTPSKKFTCQGPV-DITIQAKCNPCLSNPCKNDGTCNNDPVDFYRCTCPYGFKGQDCDVPIHACISNPCKHGGTCHLKEGENAG-FWCTCADGFEGENCEVNIDDCEDNDCENNSTCVDGINNYTCLCPPEYTGELCEEKLDFCAQDLNPCQHDSKCILTPKG-FKCDCTPGYIGEHCDIDFDDCQDNKCKNGAHCTDAVNGYTCVCPEGYSGLFCEFSP--PMVLP-----------------------RTSPCDNFDCQNGAQCI----IRINEPICQCLPGYLGEKCEKLVSVNFVNKESYLQI-PSAK--VRPQTNITLQIATDEDSGILLYKG-DKDHIAVELYRGRVRASYDTGSHPASAI-YSVETINDGNFHIVELLTLDSSLSLSVDGGSPKVITN--LSKQSTLNFDSPLYVGGMPGKNNVASLRQAPGQNGTSFHGCIRNLYINSELQDFRK-MPMQTGILPGCEPCHKKVCA------------------------------------HGMCQ--PSSQSGFTCECEEGWMGPLCDQRTN-------DPCLGNKCVHGTCLPINAFSY 1389 HSP 2 Score: 57.7658 bits (138), Expect = 1.548e-7 Identity = 42/111 (37.84%), Postives = 57/111 (51.35%), Query Frame = 1 Query: 2917 GICKNIDNYGNYECVCPVGFRGPKCTINVDD-CVGNLCVNGATCNDLNN-SYSCKCQSGFRGKYCDIKFLYCNDINPCQ----NSGQCQLLPNNDYKCVCSKGWEGKQCER 3231 G+C+ G + C C G+ GP C +D C+GN CV+G TC +N SYSCKC G G CD + + NPCQ G+C+L C C+ G+ G C+R Sbjct: 1340 GMCQPSSQSG-FTCECEEGWMGPLCDQRTNDPCLGNKCVHG-TCLPINAFSYSCKCLEGHGGVLCDEEE---DLFNPCQMIKCKHGKCRLSGVGQPYCECNSGFTGDSCDR 1445
BLAST of slit1 vs. Ensembl Mouse
Match: Slit2 (slit guidance ligand 2 [Source:MGI Symbol;Acc:MGI:1315205]) HSP 1 Score: 699.508 bits (1804), Expect = 0.000e+0 Identity = 486/1491 (32.60%), Postives = 734/1491 (49.23%), Query Frame = 1 Query: 151 EICPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQLQ------------------------CINDNIFNKLGMLKYLLLEGNKIFWISEA-ISKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCASEDKANLENMCPSDKKV-CSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQIYNFQCN----------KEFESKYETHDEECVPKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLPPIYSIHEAPNLMKLDLSFNQIETLEPDVFTRSSKLREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQ------------------------FHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCD-GNASVYHSVDSCHETNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVLSFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGIC--KNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYC-NDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNSIYF-QKNSYIEISPPSRGFLMPRGNISLNFMTNSSSGLILYQGSEQTYLVVNLYQGHLRISYNLGDKVVGPEGYSMGMVNDSRLHALHIEIVGENLTMYLNGRVHCTLKSKYLNSNRYMALDTGIFIGGAPHKILQSAIMKFHIETDSEFIGCMESLLINRKKIDFSKYLPKSINVHPGCGKLISTVVASDLKIPSIMXXXXXXXXXXXXXXXNNVCKRPGICKNQGVCEVLPGSKKKFRCICKNGFVGRKCKKRKSMCHGTFTESHIVDPDSQGTCKSIERFQY 4428 + CP C C +V+C +VP + T RL+L GN I I K DF L +L+VLQL NRI++IE GAF +L L +LRLNRN +Q P+ LF KL +LDL +NQ+Q CI D F L L+ L L N I +S A + + L+ R+H N L C+CHLAWL+ +L+Q+ + G ++ C P L ++ + EF C+ E++ + M PS + C C CS++ IV C GK L+ IP +LP + E+ L+ N+I ++ G S YK L+++ L+ N + ++ P AF GL L S+ L N + + K+ F GL SL L L N I CL D + L+LL L +NKL ++ K TF ++ ++L NP CDC + W+ DY+ T A C PR L + ++ F+C+ +++ SK +C ++CP KC C V+CSN L +P+ I T L +++N L + P L K++ S N+I +E F +S + EI L SNRL+ + ++ FK L+ L+ L L N ISC+ DSF +++ L L NQ F+C+C L W+ EW R V GN P CQ P LK+ PI + + F CD GN +SC + + C+ T V CS+ LK +P IPKD TELYLD N +L N +L + LS N I+ L+ + + L TL+LS+N+L+CI F L +L++L L ND+S +P AFNDL L+++A+G NP +CDCN++WL+ + + + GI+ CA P +M K + + F C D I AKC+ C+ PCK++G C+ Y+C CPY F G++CD I+AC PC++GG C K +N G + C C GF G C +N+DDC N C N +TC D N+Y+C C + G+ C+ K +C D+NPCQ+ +C L P +KC C+ G+ G+ C+ + DDC+ N CK+ + C D N Y C+C GY G FCE P VL S C +C N CI + + C+C PGY G C+ L S+ F K SY++I P ++ + P+ NI+L T+ SG++LY+G ++ ++ V LY+G +R SY+ G YS+ +ND H + + + +L++ ++G + + L+ + D+ +++GG P K +++ + + + F GC+ +L IN + DF K +P + PGC V A G+C+ P S+ F C C+ G++G C +R + + + + GTC I F Y Sbjct: 26 QACPAQCSCSGSTVDCHGLALRSVPRNIPRNTERLDLNGNNITRITKIDFAGLRHLRVLQLMENRISTIERGAFQDLKELERLRLNRNNLQLFPELLFLGTAKLYRLDLSENQIQAIPRKAFRGAVDIKNLQLDYNQISCIEDGAFRALRDLEVLTLNNNNITRLSVASFNHMPKLRTFRLHSNNLYCDCHLAWLSDWLRQRPRV---GLYTQ-CMGPSHLRGHNVAEVQKREFVCSDEEEGHQSFMAPSCSVLHCPAACTCSNN-IVDCRGKGLTEIPTNLPETITEIRLEQNSIRVIPPGAFSPYKKLRRLDLSNNQISELAPDAFQGLRSLNSLVLYGNKITELPKSLFEGLFSLQLLLLNANKINCLRVDAFQDLHNLNLLSLYDNKLQTVAKGTFSALRAIQTMHLAQNPFICDCHLKWLADYLHTNPIETSGARCTSPRRLANKRIGQIKSKKFRCSAKEQYFIPGTEDYRSKLSG---DCFADLACPEKCRCEGTTVDCSNQRLNKIPDHIPQYTAELRLNNNEFTVLEATGIFKKLPQLRKINFSNNKITDIEEGAFEGASGVNEILLTSNRLENVQHKMFKGLESLKTLMLRSNRISCVGNDSFIGLGSVRLLSLYDNQITTVAPGAFDSLHSLSTLNLLANPFNCNCHLAWLGEWLRRKRIVTGN---PRCQKPYFLKEIPIQDVAIQDFTCDDGNDD-----NSCSPLSRCPSECTC-----------------LDTVVRCSNKGLKVLPKGIPKDVTELYLDGNQF-TLVPKELSNYKHLTLIDLSNNRISTLSNQSFSNMTQLLTLILSYNRLRCIPPRTFDGLKSLRLLSLHGNDISVVPEGAFNDLSALSHLAIGANPLYCDCNMQWLSDWVKSEYKEPGIARCAGPGEMADKLLLTTPSKKFTCQGPV-DITIQAKCNPCLSNPCKNDGTCNNDPVDFYRCTCPYGFKGQDCDVPIHACISNPCKHGGTCHLKEGENAG-FWCTCADGFEGENCEVNIDDCEDNDCENNSTCVDGINNYTCLCPPEYTGELCEEKLDFCAQDLNPCQHDSKCILTPKG-FKCDCTPGYIGEHCDIDFDDCQDNKCKNGAHCTDAVNGYTCVCPEGYSGLFCEFSP--PMVLP-----------------------RTSPCDNFDCQNGAQCI----IRINEPICQCLPGYLGEKCEKLVSVNFVNKESYLQI-PSAK--VRPQTNITLQIATDEDSGILLYKG-DKDHIAVELYRGRVRASYDTGSHPASAI-YSVETINDGNFHIVELLTLDSSLSLSVDGGSPKVITN--LSKQSTLNFDSPLYVGGMPGKNNVASLRQAPGQNGTSFHGCIRNLYINSELQDFRK-MPMQTGILPGCEPCHKKVCA------------------------------------HGMCQ--PSSQSGFTCECEEGWMGPLCDQRTN-------DPCLGNKCVHGTCLPINAFSY 1397 HSP 2 Score: 57.7658 bits (138), Expect = 1.536e-7 Identity = 42/111 (37.84%), Postives = 57/111 (51.35%), Query Frame = 1 Query: 2917 GICKNIDNYGNYECVCPVGFRGPKCTINVDD-CVGNLCVNGATCNDLNN-SYSCKCQSGFRGKYCDIKFLYCNDINPCQ----NSGQCQLLPNNDYKCVCSKGWEGKQCER 3231 G+C+ G + C C G+ GP C +D C+GN CV+G TC +N SYSCKC G G CD + + NPCQ G+C+L C C+ G+ G C+R Sbjct: 1348 GMCQPSSQSG-FTCECEEGWMGPLCDQRTNDPCLGNKCVHG-TCLPINAFSYSCKCLEGHGGVLCDEEE---DLFNPCQMIKCKHGKCRLSGVGQPYCECNSGFTGDSCDR 1453
BLAST of slit1 vs. Ensembl Mouse
Match: Slit2 (slit guidance ligand 2 [Source:MGI Symbol;Acc:MGI:1315205]) HSP 1 Score: 695.271 bits (1793), Expect = 0.000e+0 Identity = 485/1483 (32.70%), Postives = 730/1483 (49.22%), Query Frame = 1 Query: 151 EICPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQLQ------------------------CINDNIFNKLGMLKYLLLEGNKIFWISEA-ISKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCASEDKANLENMCPSDKKV-CSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQIYNFQCN--KEFESKYETHDEECVPKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLPPIYSIHEAPNLMKLDLSFNQIETLEPDVFTRSSKLREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQ------------------------FHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCD-GNASVYHSVDSCHETNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVLSFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGIC--KNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYC-NDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNSIYF-QKNSYIEISPPSRGFLMPRGNISLNFMTNSSSGLILYQGSEQTYLVVNLYQGHLRISYNLGDKVVGPEGYSMGMVNDSRLHALHIEIVGENLTMYLNGRVHCTLKSKYLNSNRYMALDTGIFIGGAPHKILQSAIMKFHIETDSEFIGCMESLLINRKKIDFSKYLPKSINVHPGCGKLISTVVASDLKIPSIMXXXXXXXXXXXXXXXNNVCKRPGICKNQGVCEVLPGSKKKFRCICKNGFVGRKCKKRKSMCHGTFTESHIVDPDSQGTCKSIERFQY 4428 + CP C C +V+C +VP + T RL+L GN I I K DF L +L+VLQL NRI++IE GAF +L L +LRLNRN +Q P+ LF KL +LDL +NQ+Q CI D F L L+ L L N I +S A + + L+ R+H N L C+CHLAWL+ +L+Q+ + G ++ C P L ++ + EF C+ M PS + C C CS++ IV C GK L+ IP +LP + E+ L+ N+I ++ G S YK L+++ L+ N + ++ P AF GL L S+ L N + + K+ F GL SL L L N I CL D + L+LL L +NKL ++ K TF ++ ++L NP CDC + W+ DY+ T A C PR L + ++ F+C+ +++ SK +C ++CP KC C V+CSN L +P+ I T L +++N L + P L K++ S N+I +E F +S + EI L SNRL+ + ++ FK L+ L+ L L N ISC+ DSF +++ L L NQ F+C+C L W+ EW R V GN P CQ P LK+ PI + + F CD GN +SC + + C+ T V CS+ LK +P IPKD TELYLD N +L N +L + LS N I+ L+ + + L TL+LS+N+L+CI F L +L++L L ND+S +P AFNDL L+++A+G NP +CDCN++WL+ + + + GI+ CA P +M K + + F C D I AKC+ C+ PCK++G C+ Y+C CPY F G++CD I+AC PC++GG C K +N G + C C GF G C +N+DDC N C N +TC D N+Y+C C + G+ C+ K +C D+NPCQ+ +C L P +KC C+ G+ G+ C+ + DDC+ N CK+ + C D N Y C+C GY G FCE P VL S C +C N CI + + C+C PGY G C+ L S+ F K SY++I P ++ + P+ NI+L T+ SG++LY+G ++ ++ V LY+G +R SY+ G YS+ +ND H + + + +L++ ++G + + L+ + D+ +++GG P K +++ + + + F GC+ +L IN + DF K +P + PGC V A G+C+ P S+ F C C+ G++G C +R + + + + GTC I F Y Sbjct: 26 QACPAQCSCSGSTVDCHGLALRSVPRNIPRNTERLDLNGNNITRITKIDFAGLRHLRVLQLMENRISTIERGAFQDLKELERLRLNRNNLQLFPELLFLGTAKLYRLDLSENQIQAIPRKAFRGAVDIKNLQLDYNQISCIEDGAFRALRDLEVLTLNNNNITRLSVASFNHMPKLRTFRLHSNNLYCDCHLAWLSDWLRQRPRV---GLYTQ-CMGPSHLRGHNVAEVQKREFVCSGHQSF----MAPSCSVLHCPAACTCSNN-IVDCRGKGLTEIPTNLPETITEIRLEQNSIRVIPPGAFSPYKKLRRLDLSNNQISELAPDAFQGLRSLNSLVLYGNKITELPKSLFEGLFSLQLLLLNANKINCLRVDAFQDLHNLNLLSLYDNKLQTVAKGTFSALRAIQTMHLAQNPFICDCHLKWLADYLHTNPIETSGARCTSPRRLANKRIGQIKSKKFRCSGTEDYRSKLSG---DCFADLACPEKCRCEGTTVDCSNQRLNKIPDHIPQYTAELRLNNNEFTVLEATGIFKKLPQLRKINFSNNKITDIEEGAFEGASGVNEILLTSNRLENVQHKMFKGLESLKTLMLRSNRISCVGNDSFIGLGSVRLLSLYDNQITTVAPGAFDSLHSLSTLNLLANPFNCNCHLAWLGEWLRRKRIVTGN---PRCQKPYFLKEIPIQDVAIQDFTCDDGNDD-----NSCSPLSRCPSECTC-----------------LDTVVRCSNKGLKVLPKGIPKDVTELYLDGNQF-TLVPKELSNYKHLTLIDLSNNRISTLSNQSFSNMTQLLTLILSYNRLRCIPPRTFDGLKSLRLLSLHGNDISVVPEGAFNDLSALSHLAIGANPLYCDCNMQWLSDWVKSEYKEPGIARCAGPGEMADKLLLTTPSKKFTCQGPV-DITIQAKCNPCLSNPCKNDGTCNNDPVDFYRCTCPYGFKGQDCDVPIHACISNPCKHGGTCHLKEGENAG-FWCTCADGFEGENCEVNIDDCEDNDCENNSTCVDGINNYTCLCPPEYTGELCEEKLDFCAQDLNPCQHDSKCILTPKG-FKCDCTPGYIGEHCDIDFDDCQDNKCKNGAHCTDAVNGYTCVCPEGYSGLFCEFSP--PMVLP-----------------------RTSPCDNFDCQNGAQCI----IRINEPICQCLPGYLGEKCEKLVSVNFVNKESYLQI-PSAK--VRPQTNITLQIATDEDSGILLYKG-DKDHIAVELYRGRVRASYDTGSHPASAI-YSVETINDGNFHIVELLTLDSSLSLSVDGGSPKVITN--LSKQSTLNFDSPLYVGGMPGKNNVASLRQAPGQNGTSFHGCIRNLYINSELQDFRK-MPMQTGILPGCEPCHKKVCA------------------------------------HGMCQ--PSSQSGFTCECEEGWMGPLCDQRTN-------DPCLGNKCVHGTCLPINAFSY 1385 HSP 2 Score: 57.7658 bits (138), Expect = 1.534e-7 Identity = 42/111 (37.84%), Postives = 57/111 (51.35%), Query Frame = 1 Query: 2917 GICKNIDNYGNYECVCPVGFRGPKCTINVDD-CVGNLCVNGATCNDLNN-SYSCKCQSGFRGKYCDIKFLYCNDINPCQ----NSGQCQLLPNNDYKCVCSKGWEGKQCER 3231 G+C+ G + C C G+ GP C +D C+GN CV+G TC +N SYSCKC G G CD + + NPCQ G+C+L C C+ G+ G C+R Sbjct: 1336 GMCQPSSQSG-FTCECEEGWMGPLCDQRTNDPCLGNKCVHG-TCLPINAFSYSCKCLEGHGGVLCDEEE---DLFNPCQMIKCKHGKCRLSGVGQPYCECNSGFTGDSCDR 1441
BLAST of slit1 vs. Ensembl Mouse
Match: Slit2 (slit guidance ligand 2 [Source:MGI Symbol;Acc:MGI:1315205]) HSP 1 Score: 692.575 bits (1786), Expect = 0.000e+0 Identity = 486/1500 (32.40%), Postives = 735/1500 (49.00%), Query Frame = 1 Query: 151 EICPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQLQ------------------------CINDNIFNKLGMLKYLLLEGNKIFWISEA-ISKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCASEDKANLENMCPSDKKV-CSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQIYNFQCN----------KEFESKYETHDEECVPKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLPPIYSIHEAPNLMKLDLSFNQIETLEPDVFTRSSKLREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQ------------------------FHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCD-GNASVYHSVDSCHETNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVLSFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGIC--KNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGF---------RGKYCDIKFLYC-NDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNSIYF-QKNSYIEISPPSRGFLMPRGNISLNFMTNSSSGLILYQGSEQTYLVVNLYQGHLRISYNLGDKVVGPEGYSMGMVNDSRLHALHIEIVGENLTMYLNGRVHCTLKSKYLNSNRYMALDTGIFIGGAPHKILQSAIMKFHIETDSEFIGCMESLLINRKKIDFSKYLPKSINVHPGCGKLISTVVASDLKIPSIMXXXXXXXXXXXXXXXNNVCKRPGICKNQGVCEVLPGSKKKFRCICKNGFVGRKCKKRKSMCHGTFTESHIVDPDSQGTCKSIERFQY 4428 + CP C C +V+C +VP + T RL+L GN I I K DF L +L+VLQL NRI++IE GAF +L L +LRLNRN +Q P+ LF KL +LDL +NQ+Q CI D F L L+ L L N I +S A + + L+ R+H N L C+CHLAWL+ +L+Q+ + G ++ C P L ++ + EF C+ E++ + M PS + C C CS++ IV C GK L+ IP +LP + E+ L+ N+I ++ G S YK L+++ L+ N + ++ P AF GL L S+ L N + + K+ F GL SL L L N I CL D + L+LL L +NKL ++ K TF ++ ++L NP CDC + W+ DY+ T A C PR L + ++ F+C+ +++ SK +C ++CP KC C V+CSN L +P+ I T L +++N L + P L K++ S N+I +E F +S + EI L SNRL+ + ++ FK L+ L+ L L N ISC+ DSF +++ L L NQ F+C+C L W+ EW R V GN P CQ P LK+ PI + + F CD GN +SC + + C+ T V CS+ LK +P IPKD TELYLD N +L N +L + LS N I+ L+ + + L TL+LS+N+L+CI F L +L++L L ND+S +P AFNDL L+++A+G NP +CDCN++WL+ + + + GI+ CA P +M K + + F C D I AKC+ C+ PCK++G C+ Y+C CPY F G++CD I+AC PC++GG C K +N G + C C GF G C +N+DDC N C N +TC D N+Y+C C + +G+ C+ K +C D+NPCQ+ +C L P +KC C+ G+ G+ C+ + DDC+ N CK+ + C D N Y C+C GY G FCE P VL S C +C N CI + + C+C PGY G C+ L S+ F K SY++I P ++ + P+ NI+L T+ SG++LY+G ++ ++ V LY+G +R SY+ G YS+ +ND H + + + +L++ ++G + + L+ + D+ +++GG P K +++ + + + F GC+ +L IN + DF K +P + PGC V A G+C+ P S+ F C C+ G++G C +R + + + + GTC I F Y Sbjct: 26 QACPAQCSCSGSTVDCHGLALRSVPRNIPRNTERLDLNGNNITRITKIDFAGLRHLRVLQLMENRISTIERGAFQDLKELERLRLNRNNLQLFPELLFLGTAKLYRLDLSENQIQAIPRKAFRGAVDIKNLQLDYNQISCIEDGAFRALRDLEVLTLNNNNITRLSVASFNHMPKLRTFRLHSNNLYCDCHLAWLSDWLRQRPRV---GLYTQ-CMGPSHLRGHNVAEVQKREFVCSDEEEGHQSFMAPSCSVLHCPAACTCSNN-IVDCRGKGLTEIPTNLPETITEIRLEQNSIRVIPPGAFSPYKKLRRLDLSNNQISELAPDAFQGLRSLNSLVLYGNKITELPKSLFEGLFSLQLLLLNANKINCLRVDAFQDLHNLNLLSLYDNKLQTVAKGTFSALRAIQTMHLAQNPFICDCHLKWLADYLHTNPIETSGARCTSPRRLANKRIGQIKSKKFRCSAKEQYFIPGTEDYRSKLSG---DCFADLACPEKCRCEGTTVDCSNQRLNKIPDHIPQYTAELRLNNNEFTVLEATGIFKKLPQLRKINFSNNKITDIEEGAFEGASGVNEILLTSNRLENVQHKMFKGLESLKTLMLRSNRISCVGNDSFIGLGSVRLLSLYDNQITTVAPGAFDSLHSLSTLNLLANPFNCNCHLAWLGEWLRRKRIVTGN---PRCQKPYFLKEIPIQDVAIQDFTCDDGNDD-----NSCSPLSRCPSECTC-----------------LDTVVRCSNKGLKVLPKGIPKDVTELYLDGNQF-TLVPKELSNYKHLTLIDLSNNRISTLSNQSFSNMTQLLTLILSYNRLRCIPPRTFDGLKSLRLLSLHGNDISVVPEGAFNDLSALSHLAIGANPLYCDCNMQWLSDWVKSEYKEPGIARCAGPGEMADKLLLTTPSKKFTCQGPV-DITIQAKCNPCLSNPCKNDGTCNNDPVDFYRCTCPYGFKGQDCDVPIHACISNPCKHGGTCHLKEGENAG-FWCTCADGFEGENCEVNIDDCEDNDCENNSTCVDGINNYTCLCPPEYTAANLNEVEKGELCEEKLDFCAQDLNPCQHDSKCILTPKG-FKCDCTPGYIGEHCDIDFDDCQDNKCKNGAHCTDAVNGYTCVCPEGYSGLFCEFSP--PMVLP-----------------------RTSPCDNFDCQNGAQCI----IRINEPICQCLPGYLGEKCEKLVSVNFVNKESYLQI-PSAK--VRPQTNITLQIATDEDSGILLYKG-DKDHIAVELYRGRVRASYDTGSHPASAI-YSVETINDGNFHIVELLTLDSSLSLSVDGGSPKVITN--LSKQSTLNFDSPLYVGGMPGKNNVASLRQAPGQNGTSFHGCIRNLYINSELQDFRK-MPMQTGILPGCEPCHKKVCA------------------------------------HGMCQ--PSSQSGFTCECEEGWMGPLCDQRTN-------DPCLGNKCVHGTCLPINAFSY 1406 HSP 2 Score: 57.7658 bits (138), Expect = 1.462e-7 Identity = 42/111 (37.84%), Postives = 57/111 (51.35%), Query Frame = 1 Query: 2917 GICKNIDNYGNYECVCPVGFRGPKCTINVDD-CVGNLCVNGATCNDLNN-SYSCKCQSGFRGKYCDIKFLYCNDINPCQ----NSGQCQLLPNNDYKCVCSKGWEGKQCER 3231 G+C+ G + C C G+ GP C +D C+GN CV+G TC +N SYSCKC G G CD + + NPCQ G+C+L C C+ G+ G C+R Sbjct: 1357 GMCQPSSQSG-FTCECEEGWMGPLCDQRTNDPCLGNKCVHG-TCLPINAFSYSCKCLEGHGGVLCDEEE---DLFNPCQMIKCKHGKCRLSGVGQPYCECNSGFTGDSCDR 1462
BLAST of slit1 vs. UniProt/SwissProt
Match: sp|Q9WVB4|SLIT3_MOUSE (Slit homolog 3 protein OS=Mus musculus OX=10090 GN=Slit3 PE=2 SV=2) HSP 1 Score: 729.169 bits (1881), Expect = 0.000e+0 Identity = 468/1381 (33.89%), Postives = 709/1381 (51.34%), Query Frame = 1 Query: 148 SEICPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQLQ------------------------CINDNIFNKLGMLKYLLLEGNKIFWI-SEAISKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCASEDKANLENMCPSDKKVCSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQIYNFQC--NKEFESKYETHDEECVPKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLPPIYSIHEAPNLMKLDLS------------------------FNQIETLEPDVFTRSSKLREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQFHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCDGNASVYHSVDSCHETNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNE--TSFKNLLNLKTLV-LSYNGITELNKNVLTPLKSLETLVLSFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGN-YECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYC-NDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNSIYF-QKNSYIEISPPSRGFLMPRGNISLNFMTNSSSGLILYQGSEQTYLVVNLYQGHLRISYNLGDKVVGPEG--YSMGMVNDSRLHALHIEIVGENLTMYLNGRVHCTLKSKYLNSNRYMALDTGIFIGGAPHKILQSAIMKFHIETDSEFIGCMESLLINRKKIDFSKYLPKSINVHPGC 4113 + CP C C SV+C VP + RL+L N I I K DF L NL+VL L +N+++ IE GAF +L L +LRLN+N +Q LP+ LF KL +LDL +NQ+Q CI D F L L+ L L N I I + + + ++ LR+H N L C+CHLAWL+ +L+Q+ I LC P L ++ + E+ C C ++ C C+CS++ IV C GK L+ IP +LP +VE+ L+ N+I + G ++YK LK+I +++N + DI P AF GL LTS+ L N + I K F GLVSL L L N I CL +T + + L+LL L +NKL ++ K F P +++ L+L NP CDC + W+ DY++D T A C PR L + ++ F+C ++++ +++ + EC + CP KC C +V+CSN L +P + T L ++ N++ L + PNL K++LS NQ+ET+ +F S L+ + L SN + C++N+TF L + +L L +N I+ I +F ++L + L N F+C+C + W+ W R V GN P CQ P LK+ PI + + F CDGN SC + C+ T V CS+ L +P +PKD TELYL+ NHL ++ + ++F+ L TL+ LS N I+ L + + + L TL+LS+N+L+CI AF L +L+VL L ND+S++P +FNDL +L+++ALG NP HCDC+++WL+++ + + GI+ C+SPE M + + + F C D I AKC++C+ PCK+NG CS Y+C CPY + GK+C IN C PC +GG C +N + + C CP+GF G +C IN DDC N C N ATC D N+Y+C C + G+ CD YC ++N CQ+ +C L + ++C C G+ GK CE N DDC + C+H +QCVD N Y C+C G+ G FCE P P VL + S C +EC N CI Q+ C+C PG++G C+ L ++ F K+SY+E++ + P+ NISL T+ +G++LY+G + L + LYQGH+R+ Y D + P YS+ VND + H++ + ++ + L + ++ +L L + ++ +++GG P SA+ + F GC+ + IN + DF P+S+ V PGC Sbjct: 31 AAACPTKCTCSAASVDCHGLGLRAVPRGIPRNAERLDLDRNNITRITKMDFAGLKNLRVLHLEDNQVSIIERGAFQDLKQLERLRLNKNKLQVLPELLFQSTPKLTRLDLSENQIQGIPRKAFRGVTGVKNLQLDNNHISCIEDGAFRALRDLEILTLNNNNISRILVTSFNHMPKIRTLRLHSNHLYCDCHLAWLSDWLRQRRTI----GQFTLCMAPVHLRGFSVADVQKKEYVCPGPHSE--APACNANSLSCPSACSCSNN-IVDCRGKGLTEIPANLPEGIVEIRLEQNSIKSIPAGAFTQYKKLKRIDISKNQISDIAPDAFQGLKSLTSLVLYGNKITEIPKGLFDGLVSLQLLLLNANKINCLRVNTFQDLQNLNLLSLYDNKLQTISKGLFVPLQSIQTLHLAQNPFVCDCHLKWLADYLQDNPIETSGARCSSPRRLANKRISQIKSKKFRCSGSEDYRNRFSS---ECFMDLVCPEKCRCEGTIVDCSNQKLARIPSHLPEYTTDLRLNDNDISVLEATGIFKKLPNLRKINLSNNRIKEVREGAFDGAAGVQELMLTGNQLETMHGRMFRGLSSLKTLMLRSNLISCVSNDTFAGLSSVRLLSLYDNRITTITPGAFTTLVSLSTINLLSNPFNCNCHMAWLGRWLRKRRIVSGN---PRCQKPFFLKEIPIQDVAIQDFTCDGNEE-----SSCQLSPRCPEQCTC-----------------VETVVRCSNRGLHALPKGMPKDVTELYLEGNHLTAVPKELSAFRQL----TLIDLSNNSISMLTNHTFSNMSHLSTLILSYNRLRCIPVHAFNGLRSLRVLTLHGNDISSVPEGSFNDLTSLSHLALGTNPLHCDCSLRWLSEWVKAGYKEPGIARCSSPESMADRLLLTTPTHRFQCKGPV-DINIVAKCNACLSSPCKNNGTCSQDPVEQYRCTCPYSYKGKDCTVPINTCVQNPCEHGGTCHLSENLRDGFSCSCPLGFEGQRCEINPDDCEDNDCENSATCVDGINNYACLCPPNYTGELCDEVIDYCVPEMNLCQHEAKCISL-DKGFRCECVPGYSGKLCETNNDDCVAHKCRHGAQCVDEVNGYTCICPQGFSGLFCEHPP--PMVL-----------------------LQTSPCDQYECQNGAQCI----VVQQEPTCRCPPGFAGPRCEKLITVNFVGKDSYVELASAK---VRPQANISLQVATDKDNGILLYKG-DNDPLALELYQGHVRLVY---DSLSSPPTTVYSVETVNDGQFHSVELVMLNQTLNLVVDKGAPKSLGK--LQKQPAVGSNSPLYLGGIPTSTGLSALRQGADRPLGGFHGCIHEVRINNELQDFKALPPQSLGVSPGC 1332 HSP 2 Score: 186.422 bits (472), Expect = 6.309e-46 Identity = 128/479 (26.72%), Postives = 213/479 (44.47%), Query Frame = 1 Query: 1507 PKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLP-----------------------------------------------PIYSIHEAPNLMKLDLSFNQIETLEPDVFTRSSKLREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQFHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCDGNASVYHSVDSCHETNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVL------------------------SFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICS 2730 P +CP KCTCS+ V+C GL ++P I + + L + NN+ ++ P P L +LDLS NQI+ + F + ++ + L++N + CI + F+ L++LEIL L+ N IS I+ SF + ++ L L+ N +CDC L W+S+W R T+ + C +P+ L+ + + K ++C G S + ++ + S + CS N VDC L EIP +P+ E+ L++N ++S+ +F LK + +S N I+++ + LKSL +LVL + NK+ C+ + F++L NL +L L N + TI F L+++ + L QNPF CDC++KWL + D ++ + C+SP ++ K I K F CS Sbjct: 30 PAAACPTKCTCSAASVDCHGLGLRAVPRGIPRNAERLDLDRNNITRITKMDFAGLKNLRVLHLEDNQVSIIERGAFQDLKQLERLRLNKNKLQVLPELLFQSTPKLTRLDLSENQIQGIPRKAFRGVTGVKNLQLDNNHISCIEDGAFRALRDLEILTLNNNNISRILVTSFNHMPKIRTLRLHSNHLYCDCHLAWLSDWLRQRRTI---GQFTLCMAPVHLRGFSVADVQKKEYVCPGPHSEAPACNA--NSLSCPSACSCSN-----------------NIVDCRGKGLTEIPANLPEGIVEIRLEQNSIKSIPAGAFTQYKKLKRIDISKNQISDIAPDAFQGLKSLTSLVLYGNKITEIPKGLFDGLVSLQLLLLNANKINCLRVNTFQDLQNLNLLSLYDNKLQTISKGLFVPLQSIQTLHLAQNPFVCDCHLKWLADYLQDNPIETSGARCSSPRRLANKRISQIKSKKFRCS 486 HSP 3 Score: 56.225 bits (134), Expect = 3.437e-6 Identity = 39/115 (33.91%), Postives = 59/115 (51.30%), Query Frame = 1 Query: 2905 CRNGGICKNIDNYGNYECVCPVGFRGPKCTINV-DDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCND-INPCQ----NSGQCQLLPNNDYKCVCSKGWEGKQCER 3231 CR+G +C++++ + C C G+ GP C D C+G+ C +G TC +SY CKC G+ G CD K ND + C + GQC + + C+C G+ G CE+ Sbjct: 1338 CRHG-LCRSVEK-DSVVCECHPGWTGPLCDQEARDPCLGHSCRHG-TCMATGDSYVCKCAEGYGGALCDQK----NDSASACSAFKCHHGQCHISDRGEPYCLCQPGFSGHHCEQ 1445
BLAST of slit1 vs. UniProt/SwissProt
Match: sp|O88280|SLIT3_RAT (Slit homolog 3 protein OS=Rattus norvegicus OX=10116 GN=Slit3 PE=2 SV=1) HSP 1 Score: 726.472 bits (1874), Expect = 0.000e+0 Identity = 463/1378 (33.60%), Postives = 706/1378 (51.23%), Query Frame = 1 Query: 148 SEICPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQLQ------------------------CINDNIFNKLGMLKYLLLEGNKIFWI-SEAISKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCASEDKANLENMCPSDKKVCSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQIYNFQC--NKEFESKYETHDEECVPKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLPPIYSIHEAPNLMKLDLS------------------------FNQIETLEPDVFTRSSKLREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQFHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCDGNASVYHSVDSCHETNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVLSFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGN-YECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYC-NDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNSIYF-QKNSYIEISPPSRGFLMPRGNISLNFMTNSSSGLILYQGSEQTYLVVNLYQGHLRISYNLGDKVVGPEG--YSMGMVNDSRLHALHIEIVGENLTMYLNGRVHCTLKSKYLNSNRYMALDTGIFIGGAPHKILQSAIMKFHIETDSEFIGCMESLLINRKKIDFSKYLPKSINVHPGC 4113 + CP C C SV+C VP + RL+L N I I K DF L NL+VL L +N+++ IE GAF +L L +LRLN+N +Q LP+ LF KL +LDL +NQ+Q CI D F L L+ L L N I I + + + ++ LR+H N L C+CHLAWL+ +L+Q+ I LC P L ++ + E+ C C ++ C C+CS++ IV C GK L+ IP +LP +VE+ L+ N+I + G +YK LK+I +++N + DI P AF GL LTS+ L N + I K F GLVSL L L N I CL +T + + L+LL L +NKL ++ K F P +++ L+L NP CDC + W+ DY++D T A C PR L + ++ F+C ++++ +++ + EC + CP KC C +V+CSN L +P + T L ++ N++ L + PNL K++LS NQ+ET+ +F S L+ + L SN + C+NN+TF L + +L L +N I+ I +F ++L + L N F+C+C + W+ W R V GN P CQ P LK+ PI + + F C+GN +SC + C+ T V CS+ L +P +PKD TELYL+ NHL ++ + L + LS N I+ L + + + L TL+LS+N+L+CI AF L +L+VL L ND+S++P +FNDL +L+++ALG NP HCDC+++WL+++ + + GI+ C+SPE M + + + F C D I AKC++C+ PCK+NG CS Y+C CPY + GK+C IN C PC++GG C +++ + + C CP+GF G +C IN DDC N C N ATC D N+Y+C C + G+ CD YC ++N CQ+ +C L + ++C C G+ GK CE + DDC + C+H +QCVD N Y C+C G+ G FCE P P VL + S C +EC N CI Q+ C+C PG++G C+ L ++ F K+SY+E++ + P+ NISL T+ +G++LY+G + L + LYQGH+R+ Y D + P YS+ VND + H++ + ++ + L + ++ +L L + +++ +++GG P SA+ + F GC+ + IN + DF P+S+ V PGC Sbjct: 31 AAACPTKCTCSAASVDCHGLGLRAVPRGIPRNAERLDLDRNNITRITKMDFTGLKNLRVLHLEDNQVSVIERGAFQDLKQLERLRLNKNKLQVLPELLFQSTPKLTRLDLSENQIQGIPRKAFRGVTGVKNLQLDNNHISCIEDGAFRALRDLEILTLNNNNISRILVTSFNHMPKIRTLRLHSNHLYCDCHLAWLSDWLRQRRTI----GQFTLCMAPVHLRGFSVADVQKKEYVCPGPHSE--APACNANSLSCPSACSCSNN-IVDCRGKGLTEIPANLPEGIVEIRLEQNSIKSIPAGAFIQYKKLKRIDISKNQISDIAPDAFQGLKSLTSLVLYGNKITEIPKGLFDGLVSLQLLLLNANKINCLRVNTFQDLQNLNLLSLYDNKLQTISKGLFAPLQSIQTLHLAQNPFVCDCHLKWLADYLQDNPIETSGARCSSPRRLANKRISQIKSKKFRCSGSEDYRNRFSS---ECFMDLVCPEKCRCEGTIVDCSNQKLSRIPSHLPEYTTDLRLNDNDIAVLEATGIFKKLPNLRKINLSNNRIKEVREGAFDGAAGVQELMLTGNQLETMHGRMFRGLSGLKTLMLRSNLISCVNNDTFAGLSSVRLLSLYDNRITTISPGAFTTLVSLSTINLLSNPFNCNCHMAWLGRWLRKRRIVSGN---PRCQKPFFLKEIPIQDVAIQDFTCEGNEE-----NSCQLSPRCPEQCTC-----------------VETVVRCSNRGLHTLPKGMPKDVTELYLEGNHLTAVPK-ELSTFRQLTLIDLSNNSISMLTNHTFSNMSHLSTLILSYNRLRCIPVHAFNGLRSLRVLTLHGNDISSVPEGSFNDLTSLSHLALGINPLHCDCSLRWLSEWIKAGYKEPGIARCSSPESMADRLLLTTPTHRFQCKGPV-DINIVAKCNACLSSPCKNNGTCSQDPVEQYRCTCPYSYKGKDCTVPINTCVQNPCQHGGTCHLSESHRDGFSCSCPLGFEGQRCEINPDDCEDNDCENSATCVDGINNYACVCPPNYTGELCDEVIDYCVPEMNLCQHEAKCISL-DKGFRCECVPGYSGKLCETDNDDCVAHKCRHGAQCVDAVNGYTCICPQGFSGLFCEHPP--PMVL-----------------------LQTSPCDQYECQNGAQCI----VVQQEPTCRCPPGFAGPRCEKLITVNFVGKDSYVELASAK---VRPQANISLQVATDKDNGILLYKG-DNDPLALELYQGHVRLVY---DSLSSPPTTVYSVETVNDGQFHSVELVMLNQTLNLVVDKGAPKSLGK--LQKQPAVGINSPLYLGGIPTSTGLSALRQGADRPLGGFHGCIHEVRINNELQDFKALPPQSLGVSPGC 1332 HSP 2 Score: 185.652 bits (470), Expect = 1.060e-45 Identity = 128/479 (26.72%), Postives = 213/479 (44.47%), Query Frame = 1 Query: 1507 PKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLP-----------------------------------------------PIYSIHEAPNLMKLDLSFNQIETLEPDVFTRSSKLREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQFHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCDGNASVYHSVDSCHETNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVL------------------------SFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICS 2730 P +CP KCTCS+ V+C GL ++P I + + L + NN+ ++ P P L +LDLS NQI+ + F + ++ + L++N + CI + F+ L++LEIL L+ N IS I+ SF + ++ L L+ N +CDC L W+S+W R T+ + C +P+ L+ + + K ++C G S + ++ + S + CS N VDC L EIP +P+ E+ L++N ++S+ +F LK + +S N I+++ + LKSL +LVL + NK+ C+ + F++L NL +L L N + TI F L+++ + L QNPF CDC++KWL + D ++ + C+SP ++ K I K F CS Sbjct: 30 PAAACPTKCTCSAASVDCHGLGLRAVPRGIPRNAERLDLDRNNITRITKMDFTGLKNLRVLHLEDNQVSVIERGAFQDLKQLERLRLNKNKLQVLPELLFQSTPKLTRLDLSENQIQGIPRKAFRGVTGVKNLQLDNNHISCIEDGAFRALRDLEILTLNNNNISRILVTSFNHMPKIRTLRLHSNHLYCDCHLAWLSDWLRQRRTI---GQFTLCMAPVHLRGFSVADVQKKEYVCPGPHSEAPACNA--NSLSCPSACSCSN-----------------NIVDCRGKGLTEIPANLPEGIVEIRLEQNSIKSIPAGAFIQYKKLKRIDISKNQISDIAPDAFQGLKSLTSLVLYGNKITEIPKGLFDGLVSLQLLLLNANKINCLRVNTFQDLQNLNLLSLYDNKLQTISKGLFAPLQSIQTLHLAQNPFVCDCHLKWLADYLQDNPIETSGARCSSPRRLANKRISQIKSKKFRCS 486 HSP 3 Score: 60.077 bits (144), Expect = 2.373e-7 Identity = 41/115 (35.65%), Postives = 59/115 (51.30%), Query Frame = 1 Query: 2905 CRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDD-CVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCND-INPCQ----NSGQCQLLPNNDYKCVCSKGWEGKQCER 3231 CR+G +C++++ + C C G+ GP C D C+G+ C +G TC NSY CKC G+ G CD K ND N C + GQC + + C+C G+ G CE+ Sbjct: 1338 CRHG-LCRSVEK-DSVVCECHPGWTGPLCDQEAQDPCLGHSCSHG-TCVATGNSYVCKCAEGYEGPLCDQK----NDSANACSAFKCHHGQCHISDRGEPYCLCQPGFSGNHCEQ 1445
BLAST of slit1 vs. UniProt/SwissProt
Match: sp|P24014|SLIT_DROME (Protein slit OS=Drosophila melanogaster OX=7227 GN=sli PE=1 SV=2) HSP 1 Score: 726.087 bits (1873), Expect = 0.000e+0 Identity = 484/1534 (31.55%), Postives = 751/1534 (48.96%), Query Frame = 1 Query: 157 CPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQLQCINDNIFNKLGMLKYLLLEGNKIFWISEAISK-------------------------LKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCAS-EDKANLENMCPSDKKVCSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQIYNFQCN-KEFESKYETHDEECVPKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLPPIYSIHEAPNLMKLDLSFNQIETLEPDVFTRSSKLREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQFHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCDGNASVYHSVDSCHETNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVLSFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNL-CVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCN-DINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNSIYF-QKNSYIEISPPSRGFLMPRGNISLNFMTNSSSGLILYQGSEQTYLVVNLYQGHLRISYNLGDKVVGPEGYSMGMVNDSRLHALHIEIVGENLTMYLNGRVHCTLKSKYLN--SNRYMALDTGIFIGGAPHKILQSAIMKFHIETDSEFIGCMESLLINRKKIDFSKYLPKSINVHPGCGKLISTVVASDLKIPSIMXXXXXXXXXXXXXXXNNVCKRPGICKNQGVCEVLPGSKKK--FRCICKNGFVGRKCKKRKS-------MCHGTFTESHIVDPDSQGTCKSIERFQYRICTGTCSTSRMQSDVIKNFYHKPIDPSSSRCCQPTKYSTHEIQFKCENGRVYRRAANLPKNCKCVANC 4635 CP C C +V+C HR +VP + RL L GN + VI ++DF +L L++LQL++N+I +IE +F +LV+L +LRLN N ++ +P++ + L +LD+ +N + + +F L+ L L+ N+I + E K L L+ LR+ N C+CHL+WL+ FL+ + + A C +P +L ++ L EF+C+ + A +E C ++ C C C+ DGIV C K L+++P LP + EL L+ N IT L S ++ L++I L+ N + I A GL QLT++ L N +K + F GL SL L L N+I C+ KD L LL L +N + SL TF +++ ++L NP CDC + W+ DY+ T A C P+ + + +L+ F+C+ E K EC CP C C V+C+ GL +P I T L+++ N L ++ P+L+KL+L NQ+ +EP+ F +S ++E+ L N++K I+N+ F L +L+ L L +N ISC++ SF++ +L L L N F+C+C L W +EW R G R C +P ++D I L F C S + C C+ T V CS QLKEIP IP +T+ELYL+ N ++ ++ ++L +L L LS N IT L+ L L TL++S+NKLQC+ + A L+NL+VL L N +S +P +F DLK+L +IALG NP +CDC +KW + + +++ GI+ CA PE+MK K I + + F+C + I AKC++C ++PC++ +C + YQC C +HGK+C+ I+AC+G PCRN C ++ G + C C G+ G +C N+DDC+G + C N ATC D SY C+CQ GF G++CD K +C+ + NPC N +C + Y C C G+ G C N DDC+ + C++ CVD NDY+C C Y GK+CE N M Y S C HEC +G+C + + C+C PGY+G C+YL SI F NS++E+ P P N+++ F + +G+++Y G + +L V L+ G +R+SY++G+ V YS MV D + HA+ + + +N T+ RV L +N SN Y+ L T +F+GG P Q A + I + F GCM+ + IN K +DF + + PGC L + M I + N C+R C +P S + ++C CK+G GR C + + T + + + ++ C+S + +Y C G C ++CC +++ C N R Y + ++ + C C C Sbjct: 73 CPRVCSCTGLNVDCSHRGLTSVPRKISADVERLELQGNNLTVIYETDFQRLTKLRMLQLTDNQIHTIERNSFQDLVSLERLRLNNNRLKAIPENFVTSSASLLRLDISNNVITTVGRRVFKGAQSLRSLQLDNNQITCLDEHAFKGLVELEILTLNNNNLTSLPHNIFGGLGRLRALRLSDNPFACDCHLSWLSRFLRSATRL----APYTRCQSPSQLKGQNVADLHDQEFKCSGLTEHAPME--CGAENS-CPHPCRCA-DGIVDCREKSLTSVPVTLPDDTTELRLEQNFITELPPKSFSSFRRLRRIDLSNNNISRIAHDALSGLKQLTTLVLYGNKIKDLPSGVFKGLGSLQLLLLNANEISCIRKDAFRDLHSLSLLSLYDNNIQSLANGTFDAMKSIKTVHLAKNPFICDCNLRWLADYLHKNPIETSGARCESPKRMHRRRIESLREEKFKCSWDELRMKLSG---ECRMDSDCPAMCHCEGTTVDCTGRGLKEIPRDIPLHTTELLLNDNELGRISSDGLFGRLPHLVKLELKRNQLTGIEPNAFEGASHIQELQLGENKIKEISNKMFLGLHQLKTLNLYDNQISCVMPGSFEHLNSLTSLNLASNPFNCNCHLAWFAEWLRKKSLNGGAAR---CGAPSKVRDVQIKDLPHSEFKCSSENS-----EGCLGDGYCPPSCTC-----------------TGTVVRCSRNQLKEIPRGIPAETSELYLESNEIEQIHYERIRHLRSLTRLDLSNNQITILSNYTFANLTKLSTLIISYNKLQCLQRHALSGLNNLRVLSLHGNRISMLPEGSFEDLKSLTHIALGSNPLYCDCGLKWFSDWIKLDYVEPGIARCAEPEQMKDKLILSTPSSSFVCRGRVRND-ILAKCNACFEQPCQNQAQCVALPQREYQCLCQPGYHGKHCEFMIDACYGNPCRNNATCTVLEE-GRFSCQCAPGYTGARCETNIDDCLGEIKCQNNATCIDGVESYKCECQPGFSGEFCDTKIQFCSPEFNPCANGAKC-MDHFTHYSCDCQAGFHGTNCTDNIDDCQNHMCQNGGTCVDGINDYQCRCPDDYTGKYCE----------------GHNMISMMYPQT-------SPCQNHEC-KHGVCFQPNA-QGSDYLCRCHPGYTGKWCEYLTSISFVHNNSFVELEPLR---TRPEANVTIVFSSAEQNGILMYDG-QDAHLAVELFNGRIRVSYDVGNHPVSTM-YSFEMVADGKYHAVELLAIKKNFTL----RVDRGLARSIINEGSNDYLKLTTPMFLGGLPVDPAQQAYKNWQIRNLTSFKGCMKEVWINHKLVDFGN-AQRQQKITPGCALLEGEQQEEEDDEQDFMDETPHIKEEPVDPCLENKCRRGSRC--------VPNSNARDGYQCKCKHGQRGRYCDQGEGSTEPPTVTAASTCRKEQVREYYTENDCRSRQPLKYAKCVGGC---------------------GNQCCAAKIVRRRKVRMVCSNNRKYIKNLDIVRKCGCTKKC 1503 HSP 2 Score: 175.252 bits (443), Expect = 1.342e-42 Identity = 135/492 (27.44%), Postives = 218/492 (44.31%), Query Frame = 1 Query: 1504 VPKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLK-----------------------------------------------KLPPIYSIHEAPNLMKLDLSFNQIETLEPDVFTRSSKLREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQFHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCDGNASVYHSVDSCHETNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVLSFNKLQ------------------------CIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKC 2766 + + CP C+C+ V+CS+ GL S+P I + L + NNL K P + + +L++LD+S N I T+ VF + LR + L++N++ C++ FK L ELEIL L+ N ++ + + F L+ L L+ N F CDC L W+S + R+ + R CQSP LK + L D+ F C G H+ C NS C + VDC + L +P +P DTTEL L++N + L SF + L+ + LS N I+ + + L+ LK L TLVL NK++ CI +DAF++LH+L +L L N++ ++ F+ +K++ + L +NPF CDCN++WL + ++ + C SP++M + I + F CS + ++ +C Sbjct: 68 ITEARCPRVCSCTGLNVDCSHRGLTSVPRKISADVERLELQGNNLTVIYETDFQRLTKLRMLQLTDNQIHTIERNSFQDLVSLERLRLNNNRLKAIPENFVTSSASLLRLDISNNVITTVGRRVFKGAQSLRSLQLDNNQITCLDEHAFKGLVELEILTLNNNNLTSLPHNIFGGLGRLRALRLSDNPFACDCHLSWLSRFLRSATRLAPYTR---CQSPSQLKGQNVADLHDQEFKCSG--LTEHAPMECGAENSCPHPCRCAD-----------------GIVDCREKSLTSVPVTLPDDTTELRLEQNFITELPPKSFSSFRRLRRIDLSNNNISRIAHDALSGLKQLTTLVLYGNKIKDLPSGVFKGLGSLQLLLLNANEISCIRKDAFRDLHSLSLLSLYDNNIQSLANGTFDAMKSIKTVHLAKNPFICDCNLRWLADYLHKNPIETSGARCESPKRMHRRRIESLREEKFKCSWDELRMKLSGEC 537
BLAST of slit1 vs. UniProt/SwissProt
Match: sp|O75094|SLIT3_HUMAN (Slit homolog 3 protein OS=Homo sapiens OX=9606 GN=SLIT3 PE=2 SV=3) HSP 1 Score: 723.776 bits (1867), Expect = 0.000e+0 Identity = 464/1375 (33.75%), Postives = 701/1375 (50.98%), Query Frame = 1 Query: 157 CPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQLQ------------------------CINDNIFNKLGMLKYLLLEGNKIFWI-SEAISKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCASEDKANLENMCPSDKKVCSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQIYNFQC--NKEFESKYETHDEECVPKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLPPIYSIHEAPNLMKLDLS------------------------FNQIETLEPDVFTRSSKLREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQFHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCDGNASVYHSVDSCHETNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVLSFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGN-YECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYC-NDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNSIYF-QKNSYIEISPPSRGFLMPRGNISLNFMTNSSSGLILYQGSEQTYLVVNLYQGHLRISYNLGDKVVGPEG--YSMGMVNDSRLHALHIEIVGENLTMYLNGRVHCTLKSKYLNSNRYMALDTGIFIGGAPHKILQSAIMKFHIETDSEFIGCMESLLINRKKIDFSKYLPKSINVHPGC 4113 CP C C SV+C VP + RL+L N I I K DF L NL+VL L +N+++ IE GAF +L L +LRLN+N +Q LP+ LF KL +LDL +NQ+Q CI D F L L+ L L N I I + + + ++ LR+H N L C+CHLAWL+ +L+Q+ + LC P L ++ + E+ C + C ++ C C CS++ IV C GK L IP +LP +VE+ L+ N+I + G ++YK LK+I +++N + DI P AF GL LTS+ L N + I K F GLVSL L L N I CL +T + + L+LL L +NKL ++ K F P +++ L+L NP CDC + W+ DY++D T A C PR L + ++ F+C ++++ S++ + EC + CP KC C +V+CSN L+ +P + L ++ N + L + PNL K++LS NQ+ET+ VF S L+ + L SN + C++N+TF L + +L L +N I+ I +F ++L + L N F+C+C L W+ +W R V GN P CQ P LK+ PI + + F CDGN SC + C+ T V CS+ L+ +P +PKD TELYL+ NHL ++ L +L + LS N I+ L + + L TL+LS+N+L+CI AF L +L+VL L ND+S++P +FNDL +L+++ALG NP HCDC+++WL+++ + + GI+ C+SPE M + + + F C D I AKC++C+ PCK+NG C+ Y+C CPY + GK+C IN C PC++GG C D++ + + C CP+GF G +C IN DDC N C N ATC D N+Y C C + G+ CD +C ++N CQ+ +C L + + C C G+ GK CE + DDC + C+H +QCVD N Y C C G+ G FCE P P VL + S C +EC N CI Q+ C+C PG++G C+ L ++ F K+SY+E+ + + P+ NISL T+ +G++LY+G + L + LYQGH+R+ Y D + P YS+ VND + H++ + + + L + ++ +L L + +++ +++GG P SA+ + F GC+ + IN + DF P+S+ V PGC Sbjct: 34 CPTKCTCSAASVDCHGLGLRAVPRGIPRNAERLDLDRNNITRITKMDFAGLKNLRVLHLEDNQVSVIERGAFQDLKQLERLRLNKNKLQVLPELLFQSTPKLTRLDLSENQIQGIPRKAFRGITDVKNLQLDNNHISCIEDGAFRALRDLEILTLNNNNISRILVTSFNHMPKIRTLRLHSNHLYCDCHLAWLSDWLRQRRTV----GQFTLCMAPVHLRGFNVADVQKKEYVCPAPHSE--PPSCNANSISCPSPCTCSNN-IVDCRGKGLMEIPANLPEGIVEIRLEQNSIKAIPAGAFTQYKKLKRIDISKNQISDIAPDAFQGLKSLTSLVLYGNKITEIVKGLFDGLVSLQLLLLNANKINCLRVNTFQDLQNLNLLSLYDNKLQTISKGLFAPLQSIQTLHLAQNPFVCDCHLKWLADYLQDNPIETSGARCSSPRRLANKRISQIKSKKFRCSGSEDYRSRFSS---ECFMDLVCPEKCRCEGTIVDCSNQKLVRIPSHLPEYVTDLRLNDNEVSVLEATGIFKKLPNLRKINLSNNKIKEVREGAFDGAASVQELMLTGNQLETVHGRVFRGLSGLKTLMLRSNLIGCVSNDTFAGLSSVRLLSLYDNRITTITPGAFTTLVSLSTINLLSNPFNCNCHLAWLGKWLRKRRIVSGN---PRCQKPFFLKEIPIQDVAIQDFTCDGNEE-----SSCQLSPRCPEQCTC-----------------METVVRCSNKGLRALPRGMPKDVTELYLEGNHLTAVPR-ELSALRHLTLIDLSNNSISMLTNYTFSNMSHLSTLILSYNRLRCIPVHAFNGLRSLRVLTLHGNDISSVPEGSFNDLTSLSHLALGTNPLHCDCSLRWLSEWVKAGYKEPGIARCSSPEPMADRLLLTTPTHRFQCKGPV-DINIVAKCNACLSSPCKNNGTCTQDPVELYRCACPYSYKGKDCTVPINTCIQNPCQHGGTCHLSDSHKDGFSCSCPLGFEGQRCEINPDDCEDNDCENNATCVDGINNYVCICPPNYTGELCDEVIDHCVPELNLCQHEAKCIPL-DKGFSCECVPGYSGKLCETDNDDCVAHKCRHGAQCVDTINGYTCTCPQGFSGPFCEHPP--PMVL-----------------------LQTSPCDQYECQNGAQCI----VVQQEPTCRCPPGFAGPRCEKLITVNFVGKDSYVEL---ASAKVRPQANISLQVATDKDNGILLYKG-DNDPLALELYQGHVRLVY---DSLSSPPTTVYSVETVNDGQFHSVELVTLNQTLNLVVDKGTPKSLGK--LQKQPAVGINSPLYLGGIPTSTGLSALRQGTDRPLGGFHGCIHEVRINNELQDFKALPPQSLGVSPGC 1332 HSP 2 Score: 195.282 bits (495), Expect = 1.141e-48 Identity = 140/469 (29.85%), Postives = 229/469 (48.83%), Query Frame = 1 Query: 154 ICPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSD-FLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQLQCINDNIFNKLGMLKYLLLEGNKIFWISE-AISKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCASEDKANLENMCPSDKKVCSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQIYNFQCNKEFESKYETHDEECVPKVSCPLKCTCSSDVV 1554 +CP C+CE V+C ++ +P L E T L L N ++V+ + F KL NL+ + LSNN+I + GAFD ++ +L L N ++ + +F L+ L+ L LR N + C++++ F L ++ L L N+I I+ A + L L + + N NCNCHLAWL +L+++ + + + C PF L IPI ++ +F C N E+ C + C ++C C + +V+C K L +P+ +P +V EL L+ N++T + + LS + L I L+ N + + F ++ L+++ L+ N L+ I +AF GL SL L L NDI +P+ + N LTSL +L L NPL+CDC + W+ +++K +A C P + L + FQC + C+ C TC+ D V Sbjct: 504 VCPEKCRCEGTIVDCSNQKLVRIPSHLPEYVTDLRLNDNEVSVLEATGIFKKLPNLRKINLSNNKIKEVREGAFDGAASVQELMLTGNQLETVHGRVFRGLSGLKTLMLRSNLIGCVSNDTFAGLSSVRLLSLYDNRITTITPGAFTTLVSLSTINLLSNPFNCNCHLAWLGKWLRKRRIV----SGNPRCQKPFFLKEIPIQDVAIQDFTC----DGNEESSCQLSPR-CPEQCTC-METVVRCSNKGLRALPRGMPKDVTELYLEGNHLTAVPR-ELSALRHLTLIDLSNNSISMLTNYTFSNMSHLSTLILSYNRLRCIPVHAFNGLRSLRVLTLHGNDISSVPEGSF-------------NDLTSLS-----------HLALGTNPLHCDCSLRWLSEWVKAGYKEPGIARCSSPEPMADRLLLTTPTHRFQCKGPVDINIVAKCNACL-SSPCKNNGTCTQDPV 936 HSP 3 Score: 182.956 bits (463), Expect = 6.883e-45 Identity = 128/479 (26.72%), Postives = 213/479 (44.47%), Query Frame = 1 Query: 1507 PKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLP-----------------------------------------------PIYSIHEAPNLMKLDLSFNQIETLEPDVFTRSSKLREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQFHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCDGNASVYHSVDSCHETNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVL------------------------SFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICS 2730 P V+CP KCTCS+ V+C GL ++P I + + L + NN+ ++ P P L +LDLS NQI+ + F + ++ + L++N + CI + F+ L++LEIL L+ N IS I+ SF + ++ L L+ N +CDC L W+S+W R TV + C +P+ L+ + + K ++C S S ++ + S + C+ N VDC L EIP +P+ E+ L++N ++++ +F LK + +S N I+++ + LKSL +LVL + NK+ C+ + F++L NL +L L N + TI F L+++ + L QNPF CDC++KWL + D ++ + C+SP ++ K I K F CS Sbjct: 30 PAVACPTKCTCSAASVDCHGLGLRAVPRGIPRNAERLDLDRNNITRITKMDFAGLKNLRVLHLEDNQVSVIERGAFQDLKQLERLRLNKNKLQVLPELLFQSTPKLTRLDLSENQIQGIPRKAFRGITDVKNLQLDNNHISCIEDGAFRALRDLEILTLNNNNISRILVTSFNHMPKIRTLRLHSNHLYCDCHLAWLSDWLRQRRTV---GQFTLCMAPVHLRGFNVADVQKKEYVCPAPHSEPPSCNA--NSISCPSPCTCSN-----------------NIVDCRGKGLMEIPANLPEGIVEIRLEQNSIKAIPAGAFTQYKKLKRIDISKNQISDIAPDAFQGLKSLTSLVLYGNKITEIVKGLFDGLVSLQLLLLNANKINCLRVNTFQDLQNLNLLSLYDNKLQTISKGLFAPLQSIQTLHLAQNPFVCDCHLKWLADYLQDNPIETSGARCSSPRRLANKRISQIKSKKFRCS 486
BLAST of slit1 vs. UniProt/SwissProt
Match: sp|O94813|SLIT2_HUMAN (Slit homolog 2 protein OS=Homo sapiens OX=9606 GN=SLIT2 PE=1 SV=1) HSP 1 Score: 702.205 bits (1811), Expect = 0.000e+0 Identity = 486/1491 (32.60%), Postives = 731/1491 (49.03%), Query Frame = 1 Query: 151 EICPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQLQ------------------------CINDNIFNKLGMLKYLLLEGNKIFWISEA-ISKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCASEDKANLENMCPSDKKV-CSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQIYNFQCN----------KEFESKYETHDEECVPKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLPPIYSIHEAPNLMKLDLSFNQIETLEPDVFTRSSKLREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQ------------------------FHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCD-GNASVYHSVDSCHETNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVLSFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGIC--KNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYC-NDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNSIYF-QKNSYIEISPPSRGFLMPRGNISLNFMTNSSSGLILYQGSEQTYLVVNLYQGHLRISYNLGDKVVGPEGYSMGMVNDSRLHALHIEIVGENLTMYLNGRVHCTLKSKYLNSNRYMALDTGIFIGGAPHKILQSAIMKFHIETDSEFIGCMESLLINRKKIDFSKYLPKSINVHPGCGKLISTVVASDLKIPSIMXXXXXXXXXXXXXXXNNVCKRPGICKNQGVCEVLPGSKKKFRCICKNGFVGRKCKKRKSMCHGTFTESHIVDPDSQGTCKSIERFQY 4428 + CP C C +V+C +VP + T RL+L GN I I K+DF L +L+VLQL N+I++IE GAF +L L +LRLNRN +Q P+ LF KL +LDL +NQ+Q CI D F L L+ L L N I +S A + + L+ R+H N L C+CHLAWL+ +L+Q+ + G ++ C P L ++ + EF C+ M PS + C C CS++ IV C GK L+ IP +LP + E+ L+ N I ++ G S YK L++I L+ N + ++ P AF GL L S+ L N + + K+ F GL SL L L N I CL D + L+LL L +NKL ++ K TF P ++ ++L NP CDC + W+ DY+ T A C PR L + ++ F+C+ +++ SK +C ++CP KC C V+CSN L +PE I T L +++N L + P L K++ S N+I +E F +S + EI L SNRL+ + ++ FK L+ L+ L L N I+C+ DSF +++ L L NQ F+C+C L W+ EW R V GN P CQ P LK+ PI + + F CD GN +SC + T C+ T V CS+ LK +P IP+D TELYLD N +L N +L + LS N I+ L+ + + L TL+LS+N+L+CI F L +L++L L ND+S +P AFNDL L+++A+G NP +CDCN++WL+ + + + GI+ CA P +M K + + F C D I AKC+ C+ PCK++G C+ Y+C CPY F G++CD I+AC PC++GG C K + G + C+C GF G C +NVDDC N C N +TC D N+Y+C C + G+ C+ K +C D+NPCQ+ +C L P +KC C+ G+ G+ C+ + DDC+ N CK+ + C D N Y C+C GY G FCE P VL S C +C N CI + + C+C PGY G C+ L S+ F K SY++I P ++ + P+ NI+L T+ SG++LY+G ++ ++ V LY+G +R SY+ G YS+ +ND H + + + ++L++ ++G + + L+ + D+ +++GG P K +++ + + + F GC+ +L IN + DF K +P + PGC V A G C+ P S+ F C C+ G++G C +R + + + + GTC I F Y Sbjct: 26 QACPAQCSCSGSTVDCHGLALRSVPRNIPRNTERLDLNGNNITRITKTDFAGLRHLRVLQLMENKISTIERGAFQDLKELERLRLNRNHLQLFPELLFLGTAKLYRLDLSENQIQAIPRKAFRGAVDIKNLQLDYNQISCIEDGAFRALRDLEVLTLNNNNITRLSVASFNHMPKLRTFRLHSNNLYCDCHLAWLSDWLRQRPRV---GLYTQ-CMGPSHLRGHNVAEVQKREFVCSGHQSF----MAPSCSVLHCPAACTCSNN-IVDCRGKGLTEIPTNLPETITEIRLEQNTIKVIPPGAFSPYKKLRRIDLSNNQISELAPDAFQGLRSLNSLVLYGNKITELPKSLFEGLFSLQLLLLNANKINCLRVDAFQDLHNLNLLSLYDNKLQTIAKGTFSPLRAIQTMHLAQNPFICDCHLKWLADYLHTNPIETSGARCTSPRRLANKRIGQIKSKKFRCSAKEQYFIPGTEDYRSKLSG---DCFADLACPEKCRCEGTTVDCSNQKLNKIPEHIPQYTAELRLNNNEFTVLEATGIFKKLPQLRKINFSNNKITDIEEGAFEGASGVNEILLTSNRLENVQHKMFKGLESLKTLMLRSNRITCVGNDSFIGLSSVRLLSLYDNQITTVAPGAFDTLHSLSTLNLLANPFNCNCYLAWLGEWLRKKRIVTGN---PRCQKPYFLKEIPIQDVAIQDFTCDDGNDD-----NSCSPLSRCPTECTC-----------------LDTVVRCSNKGLKVLPKGIPRDVTELYLDGNQF-TLVPKELSNYKHLTLIDLSNNRISTLSNQSFSNMTQLLTLILSYNRLRCIPPRTFDGLKSLRLLSLHGNDISVVPEGAFNDLSALSHLAIGANPLYCDCNMQWLSDWVKSEYKEPGIARCAGPGEMADKLLLTTPSKKFTCQGPV-DVNILAKCNPCLSNPCKNDGTCNSDPVDFYRCTCPYGFKGQDCDVPIHACISNPCKHGGTCHLKEGEEDG-FWCICADGFEGENCEVNVDDCEDNDCENNSTCVDGINNYTCLCPPEYTGELCEEKLDFCAQDLNPCQHDSKCILTPKG-FKCDCTPGYVGEHCDIDFDDCQDNKCKNGAHCTDAVNGYTCICPEGYSGLFCEFSP--PMVLP-----------------------RTSPCDNFDCQNGAQCI----VRINEPICQCLPGYQGEKCEKLVSVNFINKESYLQI-PSAK--VRPQTNITLQIATDEDSGILLYKG-DKDHIAVELYRGRVRASYDTGSHPASAI-YSVETINDGNFHIVELLALDQSLSLSVDGGNPKIITN--LSKQSTLNFDSPLYVGGMPGKSNVASLRQAPGQNGTSFHGCIRNLYINSELQDFQK-VPMQTGILPGCEPCHKKVCA------------------------------------HGTCQ--PSSQAGFTCECQEGWMGPLCDQRTN-------DPCLGNKCVHGTCLPINAFSY 1393 HSP 2 Score: 58.9214 bits (141), Expect = 4.987e-7 Identity = 43/111 (38.74%), Postives = 56/111 (50.45%), Query Frame = 1 Query: 2917 GICKNIDNYGNYECVCPVGFRGPKCTINVDD-CVGNLCVNGATCNDLNN-SYSCKCQSGFRGKYCDIKFLYCNDINPCQ----NSGQCQLLPNNDYKCVCSKGWEGKQCER 3231 G C+ G + C C G+ GP C +D C+GN CV+G TC +N SYSCKC G G CD + + NPCQ G+C+L C CS G+ G C+R Sbjct: 1344 GTCQPSSQAG-FTCECQEGWMGPLCDQRTNDPCLGNKCVHG-TCLPINAFSYSCKCLEGHGGVLCDEEE---DLFNPCQAIKCKHGKCRLSGLGQPYCECSSGYTGDSCDR 1449
BLAST of slit1 vs. TrEMBL
Match: A1XCJ5 (Slit1 OS=Schmidtea mediterranea OX=79327 PE=2 SV=2) HSP 1 Score: 2967.56 bits (7692), Expect = 0.000e+0 Identity = 1525/1534 (99.41%), Postives = 1528/1534 (99.61%), Query Frame = 1 Query: 43 MIFIFKDITFKFLLVLLSSLYCFSSTIWINDPSDESEICPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQLQCINDNIFNKLGMLKYLLLEGNKIFWISEAISKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCASEDKANLENMCPSDKKVCSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQIYNFQCNKEFESKYETHDEECVPKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLPPIYSIHEAPNLMKLDLSFNQIETLEPDVFTRSSKLREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQFHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCDGNASVYHSVDSCHETNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVLSFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNSIYFQKNSYIEISPPSRGFLMPRGNISLNFMTNSSSGLILYQGSEQTYLVVNLYQGHLRISYNLGDKVVGPEGYSMGMVNDSRLHALHIEIVGENLTMYLNGRVHCTLKSKYLNSNRYMALDTGIFIGGAPHKILQSAIMKFHIETDSEFIGCMESLLINRKKIDFSKYLPKSINVHPGCGKLISTVVASDLKIPSIMXXXXXXXXXXXXXXXNNVCKRPGICKNQGVCEVLPGSKKKFRCICKNGFVGRKCKKRKSMCHGTFTESHIVDPDSQGTCKSIERFQYRICTGTCSTSRMQSDVIKNFYHKPIDPSSSRCCQPTKYSTHEIQFKCENGRVYRRAANLPKNCKCVANCLND 4644 MIFIFKDITFKFLLVLLSSLYCFSSTIWINDPSDESEICPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDNLVNLIKLRLNRNLIQYLPDDLFSKLTKLQKLDLRDNQLQCINDNIFNKLGMLKYLLLEGNKIFWISEAISKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTP LSHIPISSLSSYEFQCASEDKANLENMCPSDKKVCSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGL+SLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQIYNFQCNKEFESKYETHDEECVPKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKL PIYSIHEAPNLMKLDLSFNQIETLEPDVFTRSSKLREI+LNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFKNNLNLKYLMLNKNQFHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCDGNASV HSVDSCHETNSVKTCCSIGNEECTKSKKCPSKCKCESTKVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLE LVLSFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNSIYFQKNSYIEISPPSRGFLMPRGNISLNFMTNSSSGLILYQGSEQTYLVVNLYQGHLRISYNLGDKVVGPEGYSMGMVNDSRLHALHIEIVGENLTMYLNGRVHCTLKSKYLNSNRYMALDTGIFIGGAPHKILQSAIMKFHIETDSEFIGCMESLLINRKKIDF+KYLPKSINVHPGCGKLISTVVASDLKIPSIMQPIKQILTQTTKKKPNNVCKRPGICKNQGVCEVLPGSKKK RCICKNGFVGRKCKKRKSMCHGTFTESHIVDPDSQGTCKSIERFQYRICTGTCSTSRMQSDVIKNFYHKPIDPSSSRCCQPTKYSTHEIQFKCENGRVYRRAANLPKNCKCVANCLND Sbjct: 1 MIFIFKDITFKFLLVLLSSLYCFSSTIWINDPSDESEICPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDNLVNLIKLRLNRNLIQYLPDDLFSKLTKLQKLDLRDNQLQCINDNIFNKLGMLKYLLLEGNKIFWISEAISKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPXXLSHIPISSLSSYEFQCASEDKANLENMCPSDKKVCSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLISLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQIYNFQCNKEFESKYETHDEECVPKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLTPIYSIHEAPNLMKLDLSFNQIETLEPDVFTRSSKLREINLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFKNNLNLKYLMLNKNQFHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCDGNASVDHSVDSCHETNSVKTCCSIGNEECTKSKKCPSKCKCESTKVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLEILVLSFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNSIYFQKNSYIEISPPSRGFLMPRGNISLNFMTNSSSGLILYQGSEQTYLVVNLYQGHLRISYNLGDKVVGPEGYSMGMVNDSRLHALHIEIVGENLTMYLNGRVHCTLKSKYLNSNRYMALDTGIFIGGAPHKILQSAIMKFHIETDSEFIGCMESLLINRKKIDFTKYLPKSINVHPGCGKLISTVVASDLKIPSIMQPIKQILTQTTKKKPNNVCKRPGICKNQGVCEVLPGSKKKLRCICKNGFVGRKCKKRKSMCHGTFTESHIVDPDSQGTCKSIERFQYRICTGTCSTSRMQSDVIKNFYHKPIDPSSSRCCQPTKYSTHEIQFKCENGRVYRRAANLPKNCKCVANCLND 1534
BLAST of slit1 vs. TrEMBL
Match: E1CHK5 (Slit OS=Dugesia japonica OX=6161 GN=Djslit PE=2 SV=1) HSP 1 Score: 2213.73 bits (5735), Expect = 0.000e+0 Identity = 1120/1375 (81.45%), Postives = 1250/1375 (90.91%), Query Frame = 1 Query: 43 MIFIFKDITFKFLLVLLSSLYCFSSTIWINDPSDESEICPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQLQCINDNIFNKLGMLKYLLLEGNKIFWISEAISKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCASEDKANLENMCPSDKKVCSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQIYNFQCNKEFESKYETHDEECVPKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLPPIYSIHEAPNLMKLDLSFNQIETLEPDVFTRSSKLREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQFHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCDGNASVYHSVDSCHETNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVLSFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQS-EMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNSIYFQKNSYIEISPPSRGFLMPRGNISLNFMTNSSSGLILYQGSEQTYLVVNLYQGHLRISYNLGDKVVGPEGYSMGMVNDSRLHALHIEIVGENLTMYLNGRVHCTLKSKYLNSNRYMALDTGIFIGGAPHKILQSAIMKFHIETDSEFIGCMESLLINRKKIDFSKYLPKSINVHPGCGKLISTVVASDLKIPSI 4164 M+ IFKDIT+KF L+LL SLYCFSSTIWINDPSDESE CPNGCQCENYSVECKHRLFPNVP+ILQEKTTRLNLLGNRINVIRKSDF KL LKVLQLSNNRITSIEPGAFDNLVNLIKLRLNRNLI YLPD LFSKL+KLQKLDLRDNQLQCIN+N FNKL MLKYLLLEGNKIFWISE+ISKLK+LKELRI KNKL CNCHLAWLNWF+K + +I KP SV+CS PFRLS IP+ +LS YEFQCASEDK NLEN+C +KKVCSK+CNCS+DGIVKCVGK+LSNIPQDLP NVVELDL DNNITILQQGYLSKYK LKKI LT NGMVDIEPGAFLGL QLTS+YLN N LKTIRKNAF GL SLMFL+L N+IECLP D E K+LH+LHLSENKLT+LK+ETF P + LR+LY+V+NPLNCDCR++WIVDYIK KE N P+A+C PRN+QG LR+LQ Y+FQC+ EF+SKYETH+EECVPKVSCPLKCTC+SD+VNCSN+GL SLP SIFPSTKTLIMSHNNL KL PIYS+HEAPNLMKLDLSFNQIE L+ DVF+RS+KL +I LNSN+LKCINNETFKNL +L LQLS+N I+C+V DSFKNNLNLKYLMLNKN FHC+C +KW S+WSRNH+++LG+HRLPTCQSP +LK TP+THLD+KYFLCDGNAS+ S +SC+E +S + CCSIG++EC + KKCPSKCKCE TKVDCSD+QLKEIP+EIP+DTTELYLDRN L++LNETSFKNL+ LKTLVLSYNGITELNKNVLTPLK+LETLVLSFNKLQCIHQDAFK+L NLKVLILQSNDVSTIP+QAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFL+RFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKCD CMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNC+ +INACFGQPCRNGGICKNIDN+GNYECVCP+GFRGPKCT+N+DDCV + C+NG C DL NSYSCKCQ+GFRGKYCD KF YC+DINPC+N G C LPNNDYKC CSKGWEGK+C++N DDCKYN+CKHE++CVDLHNDYKC+CRPGY+GK+CE PAWKP + +SRR IN+S M + ENE+ISESVCAYHEC NNGIC++GDQNKNQKSYCKCKPG+SG LCQYLNS+YF KNSYIEI+PPSRGFLMP+GNISLNF+TNS+SGL+LYQG +QTYLVVNLYQGH+RISYNLGDKVVGPEGYSMGMVNDS+LH LHIEIVGENLTMYLNGRVHCTLKSKY+NSNR+MALDTGIFIGGAP+KILQSAI K+HI+TDSEFIGC+ESLLIN K+IDFSKYL +SINV PGCG L+STV+ SD++IPSI Sbjct: 1 MLIIFKDITYKFYLLLLYSLYCFSSTIWINDPSDESETCPNGCQCENYSVECKHRLFPNVPIILQEKTTRLNLLGNRINVIRKSDFFKLIYLKVLQLSNNRITSIEPGAFDNLVNLIKLRLNRNLIHYLPDGLFSKLSKLQKLDLRDNQLQCINENTFNKLTMLKYLLLEGNKIFWISESISKLKNLKELRIKKNKLTCNCHLAWLNWFMKHRPDIFKPDVPSVVCSAPFRLSDIPVYTLSPYEFQCASEDKTNLENICQQNKKVCSKQCNCSNDGIVKCVGKQLSNIPQDLPVNVVELDLSDNNITILQQGYLSKYKTLKKINLTGNGMVDIEPGAFLGLQQLTSLYLNANKLKTIRKNAFSGLSSLMFLFLHGNEIECLPADAFEGVKKLHILHLSENKLTTLKRETFNPLIKLRFLYIVNNPLNCDCRLAWIVDYIKGKENNEPIASCHYPRNMQGLALRSLQTYSFQCSNEFDSKYETHNEECVPKVSCPLKCTCTSDIVNCSNTGLSSLPSSIFPSTKTLIMSHNNLNKLTPIYSVHEAPNLMKLDLSFNQIEKLDADVFSRSTKLTKIHLNSNKLKCINNETFKNLIDLRTLQLSDNEINCVVVDSFKNNLNLKYLMLNKNPFHCNCNMKWTSDWSRNHHSILGDHRLPTCQSPGLLKGTPLTHLDEKYFLCDGNASIDQSFESCNEISSTEACCSIGSDECKQIKKCPSKCKCEITKVDCSDMQLKEIPHEIPQDTTELYLDRNDLKTLNETSFKNLVFLKTLVLSYNGITELNKNVLTPLKNLETLVLSFNKLQCIHQDAFKDLKNLKVLILQSNDVSTIPFQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLERFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKCDGCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCENKINACFGQPCRNGGICKNIDNFGNYECVCPLGFRGPKCTLNIDDCVAHQCINGE-CVDLRNSYSCKCQNGFRGKYCDTKFSYCHDINPCKNHGVCHSLPNNDYKCTCSKGWEGKECDQNIDDCKYNTCKHEAKCVDLHNDYKCLCRPGYVGKYCEQPAWKP--ISKPLSRRYINESIIMIQSSSENEIISESVCAYHECSNNGICVSGDQNKNQKSYCKCKPGFSGPLCQYLNSLYFHKNSYIEINPPSRGFLMPKGNISLNFLTNSTSGLLLYQGFDQTYLVVNLYQGHVRISYNLGDKVVGPEGYSMGMVNDSKLHKLHIEIVGENLTMYLNGRVHCTLKSKYINSNRHMALDTGIFIGGAPNKILQSAITKYHIDTDSEFIGCIESLLINEKRIDFSKYLSRSINVKPGCGDLLSTVITSDMEIPSI 1372
BLAST of slit1 vs. TrEMBL
Match: A0A4E0S2Z5 (Axon guidance protein OS=Fasciola hepatica OX=6192 GN=D915_002598 PE=4 SV=1) HSP 1 Score: 909.827 bits (2350), Expect = 0.000e+0 Identity = 583/1718 (33.93%), Postives = 842/1718 (49.01%), Query Frame = 1 Query: 157 CPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQLQCINDNIFNKLGMLKYLLLEGNKIFWIS-EAISKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCST------------PFRLSH---IPISSLSSYEFQCASEDKANLENMCPSDKKVCSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETN-TPVAACLQPRNLQGTPLRNLQIYNFQCNKEFESKYETHDEEC--VPKVSCPLKCTC-----------SSDV---------------VNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLPPIYSIHEAPNLMKLDLSFNQIETLEPDVFTRSSKLREISLNSN------------------------RLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQFHCDCKLKWMSEWSRNHYT-VLGNHRLPTCQSPIILKDTPITHLDDKYFLCDGNASVYHSVDSCHE------------TNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVLSFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFIC----------SEETEDA---------------------------------------------YITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRR--------DINQSEMTYENLENEVIS----ESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNSIYF-QKNSYIEISPPSRGFLMPRGNISLNFMTNSSSGLILYQGS---------EQTYLVVNLYQGHLRISYNLGDKVVGPEGYSMGMVNDSRLHALHIEIVGENLTMYLN-----GRVHCTLKSKYLNSN-------------RYMALDTGIFIGGAPHKILQSAIMKFHIETDSEFIGCMESLLINRKKIDFSKYLPK-------------------------SINVHPGC---GKLISTVVASDLKIPSIMXXXXXXXXXXXXXXXNNVCKRPGICKNQGVCEVLPGSKKKFRCICKNGFVGRKCKKRKSMCHGTFTESHIVDPDSQGTCKSIERFQYRICTGTCS----------------TSRMQSDVIKNFY-------HKPIDPSSSRCCQPTKYSTHEIQFKCENGRVYRRAANLPKNCKCV 4626 CP GC C + VECK+ + +P + T +L + GN ++ + S F L NLK L L+NN+I ++ F NL L +LRLN+N + LP+D+F LT+LQ+LDLR+N+L C+ NIF L L+Y+ L GN I+WIS ++ S HLK+L + N C+CH+ L F + + C T L H P L CA + A+ C C+C+ D C + L +IPQDLP +++E++L N + L + Y L+KI+L N + ++ AF GL +L+S+Y++ N L+ + F GL +L LYL N I CL + + E + L +LHLSEN++ + + +F L+YLYLVHNPL CDCRM+W+ ++ +KE T A C +P +QG +R+L C+ + + EE + K+ CP KC C SSDV V+CS +GL LPE++ +TK L + +N +++L + E P L L L N+IE ++ F + +L+++ L++N L C+NN TF L IL L+ N I CI SF + +CDC L W+ +W R+H V+ PTC P L TP+ L +F C+ CH ++S +CC+ + CP C CE+ V CSDL L EIP IP TT+LYL RN +++L+ + +L L TLVLSYN + EL +NV LK+L+TLVLS N++QCIH +AF L L+VLILQ+N++S+IPY FN L LNNIALGQNP HCDCN KWLN FF +FLDNGI+LC SPE M+ KS+YHSKP DF+C S TED + +KC CM PC + G+C+ ++QC+CP P+HG+ C+ NAC PC+NGG+C+ I Y C+C GF+G +C ++ C + C NG C +NN C C +GF G C F+YC D+NPC N G+C LP N Y+C C+KGW GK C+ N DDC YN C++ + CVD N+Y C CRPGY+G +CE P W P T + RR + SE+ + ++ +S E++CAYH+C N C+ + Q C C PG+SG C++L SI +SYI I PPSRG L+PRG ISL T SSSG+++Y + YL+V+L +GH+R+ + L K E S+ +ND H + I + +NLT+ ++ + KS Y N R++AL ++ GGAP L++A + + GC+E + IN + ID ++ +I + PGC K+ T V+ + + T++ + C P +C N G C L ++C C +GF G C+K +S CH + S I DP Q +C S + R C G+CS T Q+D ++ + H+ + CCQP K + F+C +G Y R L K C C+ Sbjct: 139 CPPGCVCFSKDVECKYANWKQIPHGIPIHTEKLLITGNILSTLNASTFEGLVNLKSLILTNNQIRAVHEETFANLPRLRRLRLNQNQLSNLPEDVFRGLTQLQRLDLRENKLACLPPNIFKGLLELRYINLSGNNIYWISGDSFSDQSHLKQLDLTGNPFKCDCHIRPLIAFFLSTNQLPLLARSGATCHTHEGEVGQSSQTIGLHLLHQNNCPEGDLPKTHVACAEQAPAS---------SSCLSVCDCTVDRAANCQRRGLKSIPQDLPPDLMEINLAHNELESLPEMAFKNYPKLRKIVLDNNQITHVDGSAFNGLHELSSLYMSSNKLQALSPGLFLGLNNLKILYLHRNKISCLQQGSFEGLENLQILHLSENRIQTFPRGSFTDLRRLKYLYLVHNPLICDCRMAWLPAFLVEKEAGGTQWARCAEPLEVQGRHVRDLLPTLMSCDDSLINSHSAEGEESCDILKLKCPEKCRCTEITVTPKIQASSDVPFQLDELGLISDGLSVDCSEAGLTELPENMPVNTKDLNLRNNRIRELKADSILTELPQLNTLMLDGNRIEKIDSLAFRANRELKKLILSNNSLGSFDHRILQGMTKLQVLLLQHNELTCLNNMTFGTTSALRILMLNNNKIRCITKGSFDTMRLDSLSLSAN-PLNCDCHLSWLPDWLRDHVNQVIAGPLPPTCVEPEQLAGTPVASLARYHFTCNNTPG-----SGCHSGLGSGPPGSSPCSDSTASCCN-EEQAPVPQFGCPVGCNCEADGVYCSDLGLAEIPAGIPPGTTQLYLGRNQIKTLDPSRIGHLKKLHTLVLSYNKLRELPENVFESLKNLKTLVLSHNEIQCIHPNAFNGLSGLRVLILQANNISSIPYGTFNGLLQLNNIALGQNPLHCDCNGKWLNSFFRQQFLDNGIALCHSPENMRFKSLYHSKPGDFVCPWIRRTGQNQSSPTEDMATALFVDEVASESGTSPHEPRGWGTVRSVSSDDVARMSPEERASLAVASKCLPCMLNPCTNGGQCAARGQLDFQCNCPTPYHGRRCELEQNACSRNPCKNGGLCEVISLPSEYRCICSKGFQGERCEESIKVCSNHYCDNGGICEVVNNEQRCHCAAGFHGPRCQYAFVYCKDLNPCLNGGKCIALPENKYRCECTKGWTGKNCQTNADDCLYNRCENGASCVDGINEYICKCRPGYVGIYCERPTWNPDSYVTRMDRRIPARHLLKGFSSSEVRHSPIKIRSLSTPPLETLCAYHQCQNKATCVESHGDVYQ---CVCNPGFSGQYCEHLFSISLPSTHSYIAIVPPSRGALVPRGTISLKVSTRSSSGILVYYADLIKTGKNELPEQYLLVDLEEGHVRVMFALA-KDSNVERRSVFKINDGHAHTIQIRVESDNLTLTVDKVELPNNSYQPKKSAYPLPNEPESRTRENVTVLRHLALTNPLYFGGAPADRLEAAGRILNKDPVLGMTGCIEEVQINERPIDLARLFGAPVDKMTGAQESHSERPSAEAKQHGTAIGILPGCYTKPKMSQTAVSGSRTTELVGGEQWNSASDPTERSESTNCANPKVCLNGGTCVRLSTDTTHYKCECADGFTGPHCEKEQS-CHRSTQRSFIRDP--QTSCISTRKLTVRTCGGSCSGTGSDEDSTSEGLNDLTYTEQADATRSLWLPESIRRHRRSEKEDKICCQPIKVRYRRVHFQCVDGSAYERTVKLIKRCGCI 1833 HSP 2 Score: 146.747 bits (369), Expect = 3.039e-31 Identity = 131/489 (26.79%), Postives = 203/489 (41.51%), Query Frame = 1 Query: 1513 VSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLPPIYSIHEAPNLMKLDLSFNQIET------------------------LEPDVFTRSSKLREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQFHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCDGNASVYHSV--DSCHETNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVLSFNKLQ------------------------CIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNG-ISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKCDSC--MKRPCKHNGKCSLIT 2820 + CP C C S V C + +P I T+ L+++ N L L + NL L L+ NQI L DVF ++L+ + L N+L C+ FK L EL + LS N I I DSF + +LK L L N F CDC ++ + + L T Q P++ + H + H + ++C E + KT + + S +C LK IP ++P D E+ L N L+SL E +FKN L+ +VL N IT ++ + L L +L +S NKLQ C+ Q +F+ L NL++L L N + T P +F DL+ L + L NP CDC + WL F +++ + CA P +++ + + PT C + +++ +SC +K C +C+ IT Sbjct: 137 IYCPPGCVCFSKDVECKYANWKQIPHGIPIHTEKLLITGNILSTL-NASTFEGLVNLKSLILTNNQIRAVHEETFANLPRLRRLRLNQNQLSNLPEDVFRGLTQLQRLDLRENKLACLPPNIFKGLLELRYINLSGNNIYWISGDSFSDQSHLKQLDLTGNPFKCDCHIRPLIAFF-----------LSTNQLPLLARSGATCHTHEGEVGQSSQTIGLHLLHQNNCPEGDLPKTHVACAEQAPASSSCLSVCDCTVDRAANCQRRGLKSIPQDLPPDLMEINLAHNELESLPEMAFKNYPKLRKIVLDNNQITHVDGSAFNGLHELSSLYMSSNKLQALSPGLFLGLNNLKILYLHRNKISCLQQGSFEGLENLQILHLSENRIQTFPRGSFTDLRRLKYLYLVHNPLICDCRMAWLPAFLVEKEAGGTQWARCAEPLEVQGRHVRDLLPTLMSCDDSLINSHSAEGEESCDILKLKCPEKCRCTEIT 613
BLAST of slit1 vs. TrEMBL
Match: A0A267F2C1 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig016971g2 PE=4 SV=1) HSP 1 Score: 867.455 bits (2240), Expect = 0.000e+0 Identity = 522/1546 (33.76%), Postives = 803/1546 (51.94%), Query Frame = 1 Query: 157 CPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQL------------------------QCINDNIFNKLGMLKYLLLEGNKIFWISE-AISKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCASEDKANLENMCP-SDKKVCSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDK-ETNTPVAACLQPRNLQGTPLRNLQIYNFQCNKEFESKYETHDEECVPKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLPPIYSIHEAPNLMKLDLSFNQIETLEPDVFTRSSKLREISLNSNRLK------------------------CINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQFHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCDGNASVYHSVDSCHETNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVLSFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNS--CKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNSIYFQKNSYIEISPPSRGFLMPRGNISLNFMTNSSSGLILYQGSEQTYLVVNLYQGHLRISYNLGDKVVGPEGYSMGMVNDSRLHALHIEIVGENLTMYLNGRVHCTLKSKYLN---SNRYMALDTGIFIGGAPHKILQSAIMKFHIETDSEFIGCMESLLINRKKIDFSKYLPKSINVHPGCGKLISTVVASDLKIPSIMXXXXXXXXXXXXXXXNNVCKRPGICKNQGVCEVLPGSKKKFRCICKNGFVGRKCKKRKSMCHGTFTESHIVDPDSQGTCKSIERFQYRICTGTCSTSRMQSDVIKNFYHKPIDPSSSRCCQPTKYSTHEIQFKCENGRVYRRAANLPKNCKCV 4626 CP GC C SV C +P L + RLNL NRI++IR+SDF L L++LQL NNRI IEPG+FD L L +LRLNRN I LPD LF++ + L +LDL N+L +C+ ++ L L+ L L N I + E A S++ LK LR+ N C+C LAWL+ +L+Q+ + G H+ C+ P L ++ LSS +FQC+++ + C +++ C +KC C D V+C K LS +P+D+PTNV EL L++N IT++++G + L+++ L N + +E GAF GL L S+ L+ N+++ + + F GL L L L N + C+P+D ++ L +L L +N++ S+ + TF P +++ L + NP CDC + W+ D++++ + C P + L + F+C ++ ES+ + C CP C C D V+CS L SLP+++ +T+ L +S N ++ +P + + NL +DLS N I T+EPD F +S L ++L+ N+L+ C+N++TF+ + +L +S+N I C++ +F NL L L+ N HC+C +KW+ ++ ++ ++ N + P C+SP +LKDTP+ L ++F+C + S+ C V CCS C CP KC+CE + V C L E+P+ IP D+T+L+L +N ++ LN T L NLK L LS+N + L N+ + +LE+L +SFNKL C+ AF L LKVLILQ ND++++P+ +F+DL L I L QNP HCDCN+KWL FF R LDNG+SLCASP + +K IYH++P DF+C+ T+ + +KC+ C PC + G C +++ + CDCP F GK C R++ACFG PC N C+ + +G Y C C +GF G C N+DDC+G+ C N ATC D N+Y+C C GF G+YC+ + YC D NPCQN G+C P Y+C C GWEG+ C+ N D+C+ + C + C+D Y C CRPGY G+ CE ++ ++ + ++ VC +HECLN G CI + + C+C GY G C+ L + F +S+ I + P GNI++ T GL+LY G+ YL+++L+ G++R SY+LG G GY+ +ND + H + I E +T+ ++G T+ + Y+ + AL I+IG P +I F GC++ +L+N K ++F P + + GC N C++P +CKN+GVC+ +RC C+ F GR C+++ C + + DP++ C+S + + R C GTC S+ CCQP + I+F C GR + L + C C Sbjct: 58 CPVGCGCSGRSVSCSKAGLTTIPTELPDNVERLNLQSNRISLIRRSDFSGLTKLRILQLQNNRIERIEPGSFDELTQLERLRLNRNRITELPDGLFTRQSNLYRLDLSYNRLTFIGKDTLSGAPRLRNLQLDHNDIRCLTESALRGLERLEVLTLNRNNISTLPENAFSRMARLKTLRLADNNFICDCRLAWLSRWLRQQPQL---GLHTK-CAQPRLLFGSQVAELSSSDFQCSADHHKLYPSACAMAERANCPRKCYCQEDN-VRCNQKDLSEVPEDIPTNVQELHLENNKITLIRRGTFRRLTQLRRLFLNGNQISRLEEGAFEGLDNLYSLVLSRNSIRELPRGIFQGLKFLQLLLLDANKLTCIPEDIFHETRNLAILSLYKNEIKSIAETTFAPLRSIQQLTIAGNPFVCDCNLRWLNDFLREHPRLASSDNVCDSPDRMARKKLGEVASRKFRC-RDGESEATRNAGRCELYAECPANCKCEGDTVDCSWKRLSSLPKNLPVATRHLKLSDNMIRDVPVNSILKKLKNLQSIDLSNNLIATIEPDSFRENSNLEVLNLSGNKLRSLDPRMLSGLDKLKILQMSNNHLSCLNSDTFQETPNITVLSVSQNQIRCVMEGTFDTLQNLTSLHLDLNPLHCNCHMKWLPPFAVANHQLMRNSQ-PLCRSPDLLKDTPVVSLQSRHFVCQQDPSM-----GCQ---GVPPCCSGDAVSCDHRAFCPKKCECEGSVVRCIGQGLTEVPDHIPVDSTQLFLGQNMIEQLNFTRLNRLRNLKLLDLSHNQLYGLKSNMFANMSNLESLSVSFNKLACVEARAFAGLSKLKVLILQGNDINSLPFGSFDDLSQLTTIGLNQNPIHCDCNVKWLIDFFQRRGLDNGLSLCASPPDLAMKPIYHARPDDFVCTTATQLNH--SKCNICSTEPCLNGGTCRTVSYDRFACDCPAGFEGKRCQNRLDACFGSPCHNQAECE-VLRFGRYRCRCKIGFSGTHCETNIDDCIGHRCQNNATCLDGVNNYTCNCSPGFTGRYCETRLHYCTDYNPCQNGGECVTDPATGYRCKCVTGWEGRNCDINPDNCRRQTQPCMNGGSCIDGIGSYTCQCRPGYTGRLCERLEMASLIM-----------------DVPDAAVNRGVCTHHECLNGGRCI----QEGSEYRCQCLAGYEGKKCEKLTQVGFYDDSFARIETSR---IYPSGNITMEMATQQRQGLLLYAGNASAYLIMSLFNGYIRFSYSLG-VAPGANGYTYVKINDGKFHTVQAIINRETVTLSVDGMAK-TISATYVADAVARSVDALSEAIYIGTLPAAKRDDMQSNVNIRETFSFGGCVKRMLVNGKLVNFKLSDPTNRRLIAGCPAFAKPA---------------------------NPCEKPNLCKNRGVCQPRVEQPAGYRCKCRPNFTGRNCEEK--ACKRLASREYYTDPET--GCRSRRKLKIRRCQGTCLR------------------STGYCCQPKRLRHRMIKFLCPGGRRITKRVTLVRRCGCA 1510 HSP 2 Score: 65.855 bits (159), Expect = 9.865e-7 Identity = 57/222 (25.68%), Postives = 101/222 (45.50%), Query Frame = 1 Query: 142 DESEICPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQLQCINDNIFNKLGMLKYLLLEGNKIFWIS-EAISKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCASEDKAN 804 D CP C+CE V C + VP + +T+L L N I + + +L NLK+L LS+N++ L ++F+ ++ L+ L + N+L C+ F L LK L+L+GN I + + L L + +++N ++C+C++ WL F +++ + G LC++P L+ PI +F C + + N Sbjct: 764 DHRAFCPKKCECEGSVVRCIGQGLTEVPDHIPVDSTQLFLGQNMIEQLNFTRLNRLRNLKLLDLSHNQLYG------------------------LKSNMFANMSNLESLSVSFNKLACVEARAFAGLSKLKVLILQGNDINSLPFGSFDDLSQLTTIGLNQNPIHCDCNVKWLIDFFQRRG--LDNGLS--LCASPPDLAMKPIYHARPDDFVCTTATQLN 957
BLAST of slit1 vs. TrEMBL
Match: A0A267F5J3 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig016971g1 PE=4 SV=1) HSP 1 Score: 863.218 bits (2229), Expect = 0.000e+0 Identity = 521/1546 (33.70%), Postives = 802/1546 (51.88%), Query Frame = 1 Query: 157 CPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQL------------------------QCINDNIFNKLGMLKYLLLEGNKIFWISE-AISKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCASEDKANLENMCP-SDKKVCSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDK-ETNTPVAACLQPRNLQGTPLRNLQIYNFQCNKEFESKYETHDEECVPKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLPPIYSIHEAPNLMKLDLSFNQIETLEPDVFTRSSKLREISLNSNRLK------------------------CINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQFHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCDGNASVYHSVDSCHETNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVLSFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNS--CKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNSIYFQKNSYIEISPPSRGFLMPRGNISLNFMTNSSSGLILYQGSEQTYLVVNLYQGHLRISYNLGDKVVGPEGYSMGMVNDSRLHALHIEIVGENLTMYLNGRVHCTLKSKYLN---SNRYMALDTGIFIGGAPHKILQSAIMKFHIETDSEFIGCMESLLINRKKIDFSKYLPKSINVHPGCGKLISTVVASDLKIPSIMXXXXXXXXXXXXXXXNNVCKRPGICKNQGVCEVLPGSKKKFRCICKNGFVGRKCKKRKSMCHGTFTESHIVDPDSQGTCKSIERFQYRICTGTCSTSRMQSDVIKNFYHKPIDPSSSRCCQPTKYSTHEIQFKCENGRVYRRAANLPKNCKCV 4626 CP GC C SV C +P L + RLNL NRI++IR+SDF L L++LQL NNRI IEPG+FD L L +LRLNRN I LPD LF++ + L +LDL N+L +C+ ++ L L+ L L N I + E A S++ LK LR+ N C+C LAWL+ +L+Q+ + G H+ CS P L ++ LSS +FQC+++ + C +++ C ++C C D V+C K LS +P+D+PTNV EL L++N IT++++G + L+++ L N + +E GAF GL L S+ L+ N+++ + + F GL L L L N + C+P+D ++ L +L L +N++ S+ + TF P +++ L + NP CDC + W+ D++++ + C P + L + F+C ++ ES+ + C CP C C D V+CS L SLP+++ +T+ L +S N ++ +P + + NL +DLS N I T+EPD F +S L ++L+ N+L+ C+N++TF+ + +L +S+N I C++ +F NL L L+ N HC+C +KW+ ++ ++ ++ N + P C+SP +LKDTP+ L ++F+C + S+ C V CCS C CP KC+CE + V C L E+P+ IP D+T+L+L +N ++ LN T L NLK L LS+N + L N+ + +LE+L +SFNKL C+ AF L LKVLILQ ND++++P+ +F+DL L I L QNP HCDCN+KWL FF R LDNG+SLCASP + +K IYH++P DF+C+ T+ + +KC+ C PC + G C +++ + CDC F GK C R++ACFG PC N C+ + +G Y C C +GF G C N+DDC+G+ C N ATC D N+Y+C C GF G+YC+ + YC D NPCQN G+C P Y+C C GWEG+ C+ N D+C+ + C + C+D Y C CRPGY G+ CE ++ ++ + ++ VC +HECLN G CI + + C+C GY G C+ L + F +S+ I + P GNI++ T GL+LY G+ YL+++L+ G++R SY+LG G GY+ +ND + H + I E +T+ ++G T+ + Y+ + AL I+IG P +I F GC++ +L+N K ++F P + + GC N C++P +CKN+GVC+ +RC C+ F GR C+++ C + + DP++ C+S + + R C GTC S+ CCQP + I+F C GR + L + C C Sbjct: 58 CPVGCGCSGRSVSCSKAGLTTIPTELPDNVERLNLQSNRISLIRRSDFSGLTKLRILQLQNNRIERIEPGSFDELTQLERLRLNRNRITELPDGLFTRQSNLYRLDLSYNRLTFIGKDTLSGAPRLRNLQLDHNDIRCLTESALRGLERLEVLTLNRNNISTLPENAFSRMARLKTLRLADNNFICDCRLAWLSRWLRQQPQL---GLHTK-CSQPRLLFGSQVAELSSSDFQCSADHHKLYPSACAMAERANCPRQCYCQEDN-VRCNQKDLSEVPEDIPTNVQELHLENNKITLIRRGTFRRLTQLRRLFLNGNQISRLEEGAFEGLDNLYSLVLSRNSIRELPRGIFQGLKFLQLLLLDANKLTCIPEDIFHETRNLAILSLYKNEIKSIAETTFAPLRSIQQLTIAGNPFVCDCNLRWLNDFLREHPRLASSDNVCDSPDRMARKKLGEVASRKFRC-RDGESEATRNAGRCELYAECPANCKCEGDTVDCSWKRLTSLPKNLPVATRHLKLSDNMIRDVPVNSILKKLKNLQSIDLSNNLIATIEPDSFRENSNLEVLNLSGNKLRSLDPRMLSGLDKLKILQMSNNHLSCLNSDTFQETPNITVLSVSQNQIRCVMEGTFDTLQNLTSLHLDLNPLHCNCHMKWLPPFAVANHQLMRNSQ-PLCRSPDLLKDTPVVSLQSRHFVCQQDPSM-----GCQ---GVPPCCSGDAVSCDHRAFCPKKCECEGSVVRCIGQGLTEVPDHIPVDSTQLFLGQNLIEQLNFTRLNRLRNLKLLDLSHNQLYGLKSNMFANMSNLESLSVSFNKLACVEAGAFAGLSKLKVLILQGNDINSLPFGSFDDLSQLTTIGLNQNPIHCDCNVKWLIDFFQRRGLDNGLSLCASPPDLAMKPIYHARPDDFVCTTATQLNH--SKCNICSTEPCLNGGTCRTVSYDRFACDCQAGFEGKRCQNRLDACFGSPCHNQAECE-VLRFGRYRCRCKIGFSGTHCETNIDDCIGHRCQNNATCLDGVNNYTCNCSPGFTGRYCETRLHYCTDYNPCQNGGECVTDPATGYRCKCVTGWEGRNCDINPDNCRRQTQPCMNGGSCIDGIGSYTCQCRPGYTGRLCERLEMASLIM-----------------DVPDAAVNRGVCTHHECLNGGRCI----QEGSEYRCQCLAGYEGKKCEKLTQVGFYDDSFARIETSR---IYPSGNITMEMATQQRQGLLLYAGNASAYLIMSLFNGYIRFSYSLG-VAPGANGYTYVKINDGKFHTVQAIINRETVTLSVDGMAK-TISATYVADAVARSVDALSEAIYIGTLPAAKRDDMQSNVNIRETFSFGGCVKRMLVNGKLVNFKLSDPTNRRLIAGCPAFAKP---------------------------ENPCEKPNLCKNRGVCQPRVEQPAGYRCKCRPNFTGRNCEEK--ACKRLASREYYTDPET--GCRSRRKLKIRRCQGTCPR------------------STGYCCQPKRLRHRMIKFLCPGGRRITKRVTLVRRCGCA 1510 HSP 2 Score: 65.4698 bits (158), Expect = 1.292e-6 Identity = 57/222 (25.68%), Postives = 101/222 (45.50%), Query Frame = 1 Query: 142 DESEICPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQLQCINDNIFNKLGMLKYLLLEGNKIFWIS-EAISKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCASEDKAN 804 D CP C+CE V C + VP + +T+L L N I + + +L NLK+L LS+N++ L ++F+ ++ L+ L + N+L C+ F L LK L+L+GN I + + L L + +++N ++C+C++ WL F +++ + G LC++P L+ PI +F C + + N Sbjct: 764 DHRAFCPKKCECEGSVVRCIGQGLTEVPDHIPVDSTQLFLGQNLIEQLNFTRLNRLRNLKLLDLSHNQLYG------------------------LKSNMFANMSNLESLSVSFNKLACVEAGAFAGLSKLKVLILQGNDINSLPFGSFDDLSQLTTIGLNQNPIHCDCNVKWLIDFFQRRG--LDNGLS--LCASPPDLAMKPIYHARPDDFVCTTATQLN 957
BLAST of slit1 vs. Ensembl Cavefish
Match: slit3 (slit guidance ligand 3 [Source:NCBI gene;Acc:103037708]) HSP 1 Score: 695.271 bits (1793), Expect = 0.000e+0 Identity = 484/1553 (31.17%), Postives = 741/1553 (47.71%), Query Frame = 1 Query: 157 CPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQLQCINDNIFNKLGMLKYLLLEGNKIFWIS-EAISKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCASEDKANLENMCPSDKKVCSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQIYNFQC--NKEFESKYETHDEECVPKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLPPIYSIHEAPNLMKLDLSFNQIETLEPDVFTRS------------------------SKLREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQFHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCDG---NA---SVYHSVDSCHETNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVLSFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICK-NIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYC-NDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNSIYF-QKNSYIEISPPSRGFLMPRGNISLNFMTNSSSGLILYQGSEQTYLVVNLYQGHLRISYNLGDKVVGPEGYSMGMVNDSRLHALHIEIVGENLTMYLNGRVHCTLKSKYLNSNRYMALDTGIFIGGAPHKILQSAIMKFHIETDSEFIGCMESLLINRKKIDFSKYLPKSINVHPGCGKLISTV---------VASDLKIPSIMXXXXXXXXXXXXXXXNNVCKRPGICKNQGVCEVLPGSKKKFRCICKNGFVGRKCKKRKS--MCHGTFTESH--IVDPDSQGTCKSIERFQYRICTG--TCSTSRMQSDVIKNFYHK-------------PIDPSSSRCCQPTKYSTHEIQFKCENGRVYRRAANLPKNCKC 4623 CP C C VEC+ + F +VP + RL++ N I I K DF L NL+VL L NN+I+ IE GAF +L L +LRLNRN +Q LP+ LF KL +L L N + CI D F L L+ L L N I I + + + L+ LR+H N L+C+CHL+WL+ +L+Q+ + AH C +P + + + + +F C ++ + C VC C+C+++ IV C K L+ IP +LP +VE+ L+ N I + G S YK LK+I L++N + DI AF GL LTS+ L N + I K F GLVSL L L N I CL +T + + L+LL L +NKL ++ K F P ++ L+L NP CDC + W+ DY+ D T A C PR L + ++ F+C ++++ S+ EC + CP +C C VV+CSN L S+P + T L ++ N + L + + PNL K++LS N++ + VF + + L+ + L SN++ C++N TF L + +L L +N IS I +F +L + L N + CDC L W+ W + V GN P CQ P LK+ PI + F CDG NA SV D C +++V V CS+ L+ +P IPKDTTELYL+ N L S+ + +L L + LS N I+ L + + + L TL+LS+N+++C+ AF L +L++L L ND+STIP AFN L +L+++ALG NP +CDC ++WL+Q+ F + GI+ C P M + + + F C D + +KC C+ PC++NG C +Y C CP+ + G+NC+ NAC QPC NGG C G++ CVCP GF G +C +N +DC N C N +TC D N+Y+C C +RG C+ C +PCQ+ +C L Y+C C G+ G+ CE++ +DC N C+H ++CVD N Y C+C+ G+ G FCE P P +L + S C +C N C+ + + C+C PG+ G+ CQ +++++F K S+I++ R ++ +ISL T+ +G++LY+ + L + LYQGH+R+ Y++ K YS+ VND H + + I + L++ ++ +L L + +T ++IGG P ++ + F GC+ ++ IN + + L + PGC V + P+ T+ N+ C G C G+ + C C G+ G C R+ C G VD + TC + C +C ++ + ++ HK P CC TK +I FKC +G + +C C Sbjct: 33 CPAKCSCSGSHVECQQQGFRSVPRGIPRNAERLDVDRNNITRITKVDFSGLKNLRVLHLENNQISVIERGAFQDLKQLERLRLNRNRLQVLPELLFQSTPKLGRLQLDSNHISCIEDGAFRALRDLEILTLNNNNITLIPLSSFNHMPKLRTLRLHSNNLHCDCHLSWLSDWLRQRKGL----AHFTQCMSPAHMRGLSVPDVQKRDFVCTGPAQSEPRS-CAPQASVCPASCSCTNN-IVDCRRKGLTEIPANLPEGIVEIRLEQNLIKSIPPGAFSPYKKLKRIDLSKNHISDIADDAFSGLRSLTSLVLYGNKIAEIPKGLFDGLVSLQLLLLNANKINCLRVNTFKDLQSLNLLSLYDNKLQTISKGLFTPLRAIKTLHLAQNPFMCDCHLKWLADYLFDNPIETSGARCSHPRRLANKRISQIKGKKFRCTGSEDYRSRLTG---ECFQDLVCPERCRCEGTVVDCSNLKLTSIPPHLPEHTTDLRLNDNEISSLDATGAFKKLPNLRKINLSNNKLRDIREGVFDGAGGVLELLLTGNKLQAVQGRMFRGLTGLKTLMLRSNQITCVDNATFTGLSSVRLLSLYDNRISTIAPGAFNTLHSLSTINLLSNPYVCDCHLAWLGLWLKKTRVVSGN---PRCQRPAFLKEIPIQDVAAPDFTCDGVEENACLPSVPRCPDPCTCSDTV---------------------------VRCSNHGLRSLPKGIPKDTTELYLEGNMLTSVPK-ELSSLKQLSLVDLSNNSISSLTPFIFSNMTQLSTLILSYNQIRCVPVHAFDGLRSLRLLTLHGNDLSTIPEGAFNHLTSLSHLALGANPLYCDCELRWLSQWVKAGFKEPGIARCTGPPDMADRLLLTTPLNRFQCKGPV-DLNLMSKCAPCLATPCQNNGTCVSDVTGSYHCTCPFGYKGRNCEIAANACLSQPCINGGTCHLTPGQEGHFSCVCPPGFEGQRCEVNPNDCEDNDCENNSTCVDGINNYTCVCPPNYRGDLCEEVVDACLPGFDPCQHDSKCVPL-TRGYRCECLPGYVGQHCEQDYNDCLENKCQHGAECVDAINGYTCVCKDGFSGLFCENPP--PMIL----------------------LQKTSPCDQSDCQNGAECVLME----GEPVCRCLPGFFGNKCQKMHTVHFLGKESFIQL---PRTKILSSTHISLQVATDKDNGMLLYK-EDHDPLAIELYQGHIRLMYDIA-KYPPTTVYSVESVNDGFFHTVELLIQNQTLSLVVDKGAPKSLGK--LPRPPTVETNTRLYIGGVPSELSSPSSRSGADRQSQSFNGCIHNVRINGELQELGSGLDSVKAILPGCHACSVCAQGACRQRDDVGVTCECPAGSTGPLCDQQGTSNPCQNSRCVH-GQCVANGL---------AYSCKCAQGYSGPYCATREEPLACRGLHCVHGECRVDESGEATCACQPGYTGPTCETELSCQGEMIREQLKRHMGHKTCTSTIKVPRMECPRVCHPGLCCTSTKSRRRKITFKCSDGFTFSEELETTLDCNC 1498 HSP 2 Score: 283.108 bits (723), Expect = 7.516e-77 Identity = 207/653 (31.70%), Postives = 317/653 (48.55%), Query Frame = 1 Query: 139 SDESEICPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQLQCINDNIFNKLGMLKYLLLEGNKIFWISEAI-SKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQC-ASED-KANLENMCPSDKKVCSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQ-GYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQIYNFQCNKEFESKYETHDEECVPKVS-CPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLPPIYSIHEAPNLMKLDLSFNQIETLEPDVFTRSSKLREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQFHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCDG 2082 + ++ +CP C C N V+C+ + +P L E + L N I I F LK + LS N I+ I AF L +L L L N I +P LF L LQ L L N++ C+ N F L L L L NK+ IS+ + + L+ +K L + +N C+CHL WL +L I GA CS P RL++ IS + +F+C SED ++ L C D VC ++C C +V C +L++IP LP + +L L DN I+ L G K L+KI L+ N + DI G F G + + L N L+ ++ F GL L L L+ N I C+ T + LL L +N+++++ F +L + L+ NP CDC ++W+ ++K + C +P L+ P++++ +F C+ + C+P V CP CTCS VV CSN GL SLP+ I T L + N L +P + L +DLS N I +L P +F+ ++L + L+ N+++C+ F L+ L +L L N +S I +F + +L +L L N +CDC+L+W+S+W + + G R C P + D + F C G Sbjct: 250 APQASVCPASCSCTNNIVDCRRKGLTEIPANLPEGIVEIRLEQNLIKSIPPGAFSPYKKLKRIDLSKNHISDIADDAFSGLRSLTSLVLYGNKIAEIPKGLFDGLVSLQLLLLNANKINCLRVNTFKDLQSLNLLSLYDNKLQTISKGLFTPLRAIKTLHLAQNPFMCDCHLKWLADYLFDNP-IETSGAR---CSHPRRLANKRISQIKGKKFRCTGSEDYRSRLTGECFQD-LVCPERCRC-EGTVVDCSNLKLTSIPPHLPEHTTDLRLNDNEISSLDATGAFKKLPNLRKINLSNNKLRDIREGVFDGAGGVLELLLTGNKLQAVQGRMFRGLTGLKTLMLRSNQITCVDNATFTGLSSVRLLSLYDNRISTIAPGAFNTLHSLSTINLLSNPYVCDCHLAWLGLWLKKTRVVSGNPRCQRPAFLKEIPIQDVAAPDFTCDG-------VEENACLPSVPRCPDPCTCSDTVVRCSNHGLRSLPKGIPKDTTELYLEGNMLTSVP--KELSSLKQLSLVDLSNNSISSLTPFIFSNMTQLSTLILSYNQIRCVPVHAFDGLRSLRLLTLHGNDLSTIPEGAFNHLTSLSHLALGANPLYCDCELRWLSQWVKAGFKEPGIAR---CTGPPDMADRLLLTTPLNRFQCKG 884
BLAST of slit1 vs. Ensembl Cavefish
Match: slit3 (slit guidance ligand 3 [Source:NCBI gene;Acc:103037708]) HSP 1 Score: 688.723 bits (1776), Expect = 0.000e+0 Identity = 487/1577 (30.88%), Postives = 744/1577 (47.18%), Query Frame = 1 Query: 157 CPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQLQ------------------------CINDNIFNKLGMLKYLLLEGNKIFWIS-EAISKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCASEDKANLENMCPSDKKVCSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQIYNFQC--NKEFESKYETHDEECVPKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLPPIYSIHEAPNLMKLDLSFNQIETLEPDVFTRS------------------------SKLREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQFHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCDG---NA---SVYHSVDSCHETNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVLSFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICK-NIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYC-NDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNSIYF-QKNSYIEISPPSRGFLMPRGNISLNFMTNSSSGLILYQGSEQTYLVVNLYQGHLRISYNLGDKVVGPEGYSMGMVNDSRLHALHIEIVGENLTMYLNGRVHCTLKSKYLNSNRYMALDTGIFIGGAPHKILQSAIMKFHIETDSEFIGCMESLLINRKKIDFSKYLPKSINVHPGCGKLISTV---------VASDLKIPSIMXXXXXXXXXXXXXXXNNVCKRPGICKNQGVCEVLPGSKKKFRCICKNGFVGRKCKKRKS--MCHGTFTESH--IVDPDSQGTCKSIERFQYRICTG--TCSTSRMQSDVIKNFYHK-------------PIDPSSSRCCQPTKYSTHEIQFKCENGRVYRRAANLPKNCKC 4623 CP C C VEC+ + F +VP + RL++ N I I K DF L NL+VL L NN+I+ IE GAF +L L +LRLNRN +Q LP+ LF KL +LDL +NQ+Q CI D F L L+ L L N I I + + + L+ LR+H N L+C+CHL+WL+ +L+Q+ + AH C +P + + + + +F C ++ + C VC C+C+++ IV C K L+ IP +LP +VE+ L+ N I + G S YK LK+I L++N + DI AF GL LTS+ L N + I K F GLVSL L L N I CL +T + + L+LL L +NKL ++ K F P ++ L+L NP CDC + W+ DY+ D T A C PR L + ++ F+C ++++ S+ EC + CP +C C VV+CSN L S+P + T L ++ N + L + + PNL K++LS N++ + VF + + L+ + L SN++ C++N TF L + +L L +N IS I +F +L + L N + CDC L W+ W + V GN P CQ P LK+ PI + F CDG NA SV D C +++V V CS+ L+ +P IPKDTTELYL+ N L S+ + +L L + LS N I+ L + + + L TL+LS+N+++C+ AF L +L++L L ND+STIP AFN L +L+++ALG NP +CDC ++WL+Q+ F + GI+ C P M + + + F C D + +KC C+ PC++NG C +Y C CP+ + G+NC+ NAC QPC NGG C G++ CVCP GF G +C +N +DC N C N +TC D N+Y+C C +RG C+ C +PCQ+ +C L Y+C C G+ G+ CE++ +DC N C+H ++CVD N Y C+C+ G+ G FCE P + KT S C +C N C+ + + C+C PG+ G+ CQ +++++F K S+I++ R ++ +ISL T+ +G++LY+ + L + LYQGH+R+ Y++ K YS+ VND H + + I + L++ ++ +L L + +T ++IGG P ++ + F GC+ ++ IN + + L + PGC V + P+ T+ N+ C G C G+ + C C G+ G C R+ C G VD + TC + C +C ++ + ++ HK P CC TK +I FKC +G + +C C Sbjct: 33 CPAKCSCSGSHVECQQQGFRSVPRGIPRNAERLDVDRNNITRITKVDFSGLKNLRVLHLENNQISVIERGAFQDLKQLERLRLNRNRLQVLPELLFQSTPKLGRLDLSENQIQAIPRKSFRGITNVKNLQLDSNHISCIEDGAFRALRDLEILTLNNNNITLIPLSSFNHMPKLRTLRLHSNNLHCDCHLSWLSDWLRQRKGL----AHFTQCMSPAHMRGLSVPDVQKRDFVCTGPAQSEPRS-CAPQASVCPASCSCTNN-IVDCRRKGLTEIPANLPEGIVEIRLEQNLIKSIPPGAFSPYKKLKRIDLSKNHISDIADDAFSGLRSLTSLVLYGNKIAEIPKGLFDGLVSLQLLLLNANKINCLRVNTFKDLQSLNLLSLYDNKLQTISKGLFTPLRAIKTLHLAQNPFMCDCHLKWLADYLFDNPIETSGARCSHPRRLANKRISQIKGKKFRCTGSEDYRSRLTG---ECFQDLVCPERCRCEGTVVDCSNLKLTSIPPHLPEHTTDLRLNDNEISSLDATGAFKKLPNLRKINLSNNKLRDIREGVFDGAGGVLELLLTGNKLQAVQGRMFRGLTGLKTLMLRSNQITCVDNATFTGLSSVRLLSLYDNRISTIAPGAFNTLHSLSTINLLSNPYVCDCHLAWLGLWLKKTRVVSGN---PRCQRPAFLKEIPIQDVAAPDFTCDGVEENACLPSVPRCPDPCTCSDTV---------------------------VRCSNHGLRSLPKGIPKDTTELYLEGNMLTSVPK-ELSSLKQLSLVDLSNNSISSLTPFIFSNMTQLSTLILSYNQIRCVPVHAFDGLRSLRLLTLHGNDLSTIPEGAFNHLTSLSHLALGANPLYCDCELRWLSQWVKAGFKEPGIARCTGPPDMADRLLLTTPLNRFQCKGPV-DLNLMSKCAPCLATPCQNNGTCVSDVTGSYHCTCPFGYKGRNCEIAANACLSQPCINGGTCHLTPGQEGHFSCVCPPGFEGQRCEVNPNDCEDNDCENNSTCVDGINNYTCVCPPNYRGDLCEEVVDACLPGFDPCQHDSKCVPL-TRGYRCECLPGYVGQHCEQDYNDCLENKCQHGAECVDAINGYTCVCKDGFSGLFCENPPPMILLQKT------------------------SPCDQSDCQNGAECVLME----GEPVCRCLPGFFGNKCQKMHTVHFLGKESFIQL---PRTKILSSTHISLQVATDKDNGMLLYK-EDHDPLAIELYQGHIRLMYDIA-KYPPTTVYSVESVNDGFFHTVELLIQNQTLSLVVDKGAPKSLGK--LPRPPTVETNTRLYIGGVPSELSSPSSRSGADRQSQSFNGCIHNVRINGELQELGSGLDSVKAILPGCHACSVCAQGACRQRDDVGVTCECPAGSTGPLCDQQGTSNPCQNSRCVH-GQCVANGL---------AYSCKCAQGYSGPYCATREEPLACRGLHCVHGECRVDESGEATCACQPGYTGPTCETELSCQGEMIREQLKRHMGHKTCTSTIKVPRMECPRVCHPGLCCTSTKSRRRKITFKCSDGFTFSEELETTLDCNC 1522 HSP 2 Score: 282.722 bits (722), Expect = 1.011e-76 Identity = 207/653 (31.70%), Postives = 317/653 (48.55%), Query Frame = 1 Query: 139 SDESEICPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQLQCINDNIFNKLGMLKYLLLEGNKIFWISEAI-SKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQC-ASED-KANLENMCPSDKKVCSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQ-GYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQIYNFQCNKEFESKYETHDEECVPKVS-CPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLPPIYSIHEAPNLMKLDLSFNQIETLEPDVFTRSSKLREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQFHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCDG 2082 + ++ +CP C C N V+C+ + +P L E + L N I I F LK + LS N I+ I AF L +L L L N I +P LF L LQ L L N++ C+ N F L L L L NK+ IS+ + + L+ +K L + +N C+CHL WL +L I GA CS P RL++ IS + +F+C SED ++ L C D VC ++C C +V C +L++IP LP + +L L DN I+ L G K L+KI L+ N + DI G F G + + L N L+ ++ F GL L L L+ N I C+ T + LL L +N+++++ F +L + L+ NP CDC ++W+ ++K + C +P L+ P++++ +F C+ + C+P V CP CTCS VV CSN GL SLP+ I T L + N L +P + L +DLS N I +L P +F+ ++L + L+ N+++C+ F L+ L +L L N +S I +F + +L +L L N +CDC+L+W+S+W + + G R C P + D + F C G Sbjct: 274 APQASVCPASCSCTNNIVDCRRKGLTEIPANLPEGIVEIRLEQNLIKSIPPGAFSPYKKLKRIDLSKNHISDIADDAFSGLRSLTSLVLYGNKIAEIPKGLFDGLVSLQLLLLNANKINCLRVNTFKDLQSLNLLSLYDNKLQTISKGLFTPLRAIKTLHLAQNPFMCDCHLKWLADYLFDNP-IETSGAR---CSHPRRLANKRISQIKGKKFRCTGSEDYRSRLTGECFQD-LVCPERCRC-EGTVVDCSNLKLTSIPPHLPEHTTDLRLNDNEISSLDATGAFKKLPNLRKINLSNNKLRDIREGVFDGAGGVLELLLTGNKLQAVQGRMFRGLTGLKTLMLRSNQITCVDNATFTGLSSVRLLSLYDNRISTIAPGAFNTLHSLSTINLLSNPYVCDCHLAWLGLWLKKTRVVSGNPRCQRPAFLKEIPIQDVAAPDFTCDG-------VEENACLPSVPRCPDPCTCSDTVVRCSNHGLRSLPKGIPKDTTELYLEGNMLTSVP--KELSSLKQLSLVDLSNNSISSLTPFIFSNMTQLSTLILSYNQIRCVPVHAFDGLRSLRLLTLHGNDLSTIPEGAFNHLTSLSHLALGANPLYCDCELRWLSQWVKAGFKEPGIAR---CTGPPDMADRLLLTTPLNRFQCKG 908
BLAST of slit1 vs. Ensembl Cavefish
Match: slit3 (slit guidance ligand 3 [Source:NCBI gene;Acc:103037708]) HSP 1 Score: 683.715 bits (1763), Expect = 0.000e+0 Identity = 484/1553 (31.17%), Postives = 743/1553 (47.84%), Query Frame = 1 Query: 157 CPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQLQCINDNIFNKLGMLKYLLLEGNKIFWIS-EAISKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCASEDKANLENMCPSDKKVCSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQIYNFQC--NKEFESKYETHDEECVPKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLPPIYSIHEAPNLMKLDLSFNQIETLEPDVFTRS------------------------SKLREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQFHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCDG---NA---SVYHSVDSCHETNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVLSFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICK-NIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYC-NDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNSIYF-QKNSYIEISPPSRGFLMPRGNISLNFMTNSSSGLILYQGSEQTYLVVNLYQGHLRISYNLGDKVVGPEGYSMGMVNDSRLHALHIEIVGENLTMYLNGRVHCTLKSKYLNSNRYMALDTGIFIGGAPHKILQSAIMKFHIETDSEFIGCMESLLINRKKIDFSKYLPKSINVHPGCGKLISTV---------VASDLKIPSIMXXXXXXXXXXXXXXXNNVCKRPGICKNQGVCEVLPGSKKKFRCICKNGFVGRKCKKRKS--MCHGTFTESH--IVDPDSQGTCKSIERFQYRICTG--TCSTSRMQSDVIKNFYHK-------------PIDPSSSRCCQPTKYSTHEIQFKCENGRVYRRAANLPKNCKC 4623 CP C C VEC+ + F +VP + RL++ N I I K DF L NL+VL L NN+I+ IE GAF +L L +LRLNRN +Q LP+ LF KL +LDL +NQ+Q I F + +K L L N I I + + + L+ LR+H N L+C+CHL+WL+ +L+Q+ + AH C +P + + + + +F C ++ + C VC C+C+++ IV C K L+ IP +LP +VE+ L+ N I + G S YK LK+I L++N + DI AF GL LTS+ L N + I K F GLVSL L L N I CL +T + + L+LL L +NKL ++ K F P ++ L+L NP CDC + W+ DY+ D T A C PR L + ++ F+C ++++ S+ EC + CP +C C VV+CSN L S+P + T L ++ N + L + + PNL K++LS N++ + VF + + L+ + L SN++ C++N TF L + +L L +N IS I +F +L + L N + CDC L W+ W + V GN P CQ P LK+ PI + F CDG NA SV D C +++V V CS+ L+ +P IPKDTTELYL+ N L S+ + +L L + LS N I+ L + + + L TL+LS+N+++C+ AF L +L++L L ND+STIP AFN L +L+++ALG NP +CDC ++WL+Q+ F + GI+ C P M + + + F C D + +KC C+ PC++NG C +Y C CP+ + G+NC+ NAC QPC NGG C G++ CVCP GF G +C +N +DC N C N +TC D N+Y+C C +RG C+ C +PCQ+ +C L Y+C C G+ G+ CE++ +DC N C+H ++CVD N Y C+C+ G+ G FCE P P +L + S C +C N C+ + + C+C PG+ G+ CQ +++++F K S+I++ R ++ +ISL T+ +G++LY+ + L + LYQGH+R+ Y++ K YS+ VND H + + I + L++ ++ +L L + +T ++IGG P ++ + F GC+ ++ IN + + L + PGC V + P+ T+ N+ C G C G+ + C C G+ G C R+ C G VD + TC + C +C ++ + ++ HK P CC TK +I FKC +G + +C C Sbjct: 33 CPAKCSCSGSHVECQQQGFRSVPRGIPRNAERLDVDRNNITRITKVDFSGLKNLRVLHLENNQISVIERGAFQDLKQLERLRLNRNRLQVLPELLFQSTPKLGRLDLSENQIQAIPRKSFRGITNVKNLTLNNNNITLIPLSSFNHMPKLRTLRLHSNNLHCDCHLSWLSDWLRQRKGL----AHFTQCMSPAHMRGLSVPDVQKRDFVCTGPAQSEPRS-CAPQASVCPASCSCTNN-IVDCRRKGLTEIPANLPEGIVEIRLEQNLIKSIPPGAFSPYKKLKRIDLSKNHISDIADDAFSGLRSLTSLVLYGNKIAEIPKGLFDGLVSLQLLLLNANKINCLRVNTFKDLQSLNLLSLYDNKLQTISKGLFTPLRAIKTLHLAQNPFMCDCHLKWLADYLFDNPIETSGARCSHPRRLANKRISQIKGKKFRCTGSEDYRSRLTG---ECFQDLVCPERCRCEGTVVDCSNLKLTSIPPHLPEHTTDLRLNDNEISSLDATGAFKKLPNLRKINLSNNKLRDIREGVFDGAGGVLELLLTGNKLQAVQGRMFRGLTGLKTLMLRSNQITCVDNATFTGLSSVRLLSLYDNRISTIAPGAFNTLHSLSTINLLSNPYVCDCHLAWLGLWLKKTRVVSGN---PRCQRPAFLKEIPIQDVAAPDFTCDGVEENACLPSVPRCPDPCTCSDTV---------------------------VRCSNHGLRSLPKGIPKDTTELYLEGNMLTSVPK-ELSSLKQLSLVDLSNNSISSLTPFIFSNMTQLSTLILSYNQIRCVPVHAFDGLRSLRLLTLHGNDLSTIPEGAFNHLTSLSHLALGANPLYCDCELRWLSQWVKAGFKEPGIARCTGPPDMADRLLLTTPLNRFQCKGPV-DLNLMSKCAPCLATPCQNNGTCVSDVTGSYHCTCPFGYKGRNCEIAANACLSQPCINGGTCHLTPGQEGHFSCVCPPGFEGQRCEVNPNDCEDNDCENNSTCVDGINNYTCVCPPNYRGDLCEEVVDACLPGFDPCQHDSKCVPL-TRGYRCECLPGYVGQHCEQDYNDCLENKCQHGAECVDAINGYTCVCKDGFSGLFCENPP--PMIL----------------------LQKTSPCDQSDCQNGAECVLME----GEPVCRCLPGFFGNKCQKMHTVHFLGKESFIQL---PRTKILSSTHISLQVATDKDNGMLLYK-EDHDPLAIELYQGHIRLMYDIA-KYPPTTVYSVESVNDGFFHTVELLIQNQTLSLVVDKGAPKSLGK--LPRPPTVETNTRLYIGGVPSELSSPSSRSGADRQSQSFNGCIHNVRINGELQELGSGLDSVKAILPGCHACSVCAQGACRQRDDVGVTCECPAGSTGPLCDQQGTSNPCQNSRCVH-GQCVANGL---------AYSCKCAQGYSGPYCATREEPLACRGLHCVHGECRVDESGEATCACQPGYTGPTCETELSCQGEMIREQLKRHMGHKTCTSTIKVPRMECPRVCHPGLCCTSTKSRRRKITFKCSDGFTFSEELETTLDCNC 1498 HSP 2 Score: 283.108 bits (723), Expect = 7.919e-77 Identity = 207/653 (31.70%), Postives = 317/653 (48.55%), Query Frame = 1 Query: 139 SDESEICPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQLQCINDNIFNKLGMLKYLLLEGNKIFWISEAI-SKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQC-ASED-KANLENMCPSDKKVCSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQ-GYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQIYNFQCNKEFESKYETHDEECVPKVS-CPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLPPIYSIHEAPNLMKLDLSFNQIETLEPDVFTRSSKLREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQFHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCDG 2082 + ++ +CP C C N V+C+ + +P L E + L N I I F LK + LS N I+ I AF L +L L L N I +P LF L LQ L L N++ C+ N F L L L L NK+ IS+ + + L+ +K L + +N C+CHL WL +L I GA CS P RL++ IS + +F+C SED ++ L C D VC ++C C +V C +L++IP LP + +L L DN I+ L G K L+KI L+ N + DI G F G + + L N L+ ++ F GL L L L+ N I C+ T + LL L +N+++++ F +L + L+ NP CDC ++W+ ++K + C +P L+ P++++ +F C+ + C+P V CP CTCS VV CSN GL SLP+ I T L + N L +P + L +DLS N I +L P +F+ ++L + L+ N+++C+ F L+ L +L L N +S I +F + +L +L L N +CDC+L+W+S+W + + G R C P + D + F C G Sbjct: 250 APQASVCPASCSCTNNIVDCRRKGLTEIPANLPEGIVEIRLEQNLIKSIPPGAFSPYKKLKRIDLSKNHISDIADDAFSGLRSLTSLVLYGNKIAEIPKGLFDGLVSLQLLLLNANKINCLRVNTFKDLQSLNLLSLYDNKLQTISKGLFTPLRAIKTLHLAQNPFMCDCHLKWLADYLFDNP-IETSGAR---CSHPRRLANKRISQIKGKKFRCTGSEDYRSRLTGECFQD-LVCPERCRC-EGTVVDCSNLKLTSIPPHLPEHTTDLRLNDNEISSLDATGAFKKLPNLRKINLSNNKLRDIREGVFDGAGGVLELLLTGNKLQAVQGRMFRGLTGLKTLMLRSNQITCVDNATFTGLSSVRLLSLYDNRISTIAPGAFNTLHSLSTINLLSNPYVCDCHLAWLGLWLKKTRVVSGNPRCQRPAFLKEIPIQDVAAPDFTCDG-------VEENACLPSVPRCPDPCTCSDTVVRCSNHGLRSLPKGIPKDTTELYLEGNMLTSVP--KELSSLKQLSLVDLSNNSISSLTPFIFSNMTQLSTLILSYNQIRCVPVHAFDGLRSLRLLTLHGNDLSTIPEGAFNHLTSLSHLALGANPLYCDCELRWLSQWVKAGFKEPGIAR---CTGPPDMADRLLLTTPLNRFQCKG 884
BLAST of slit1 vs. Ensembl Cavefish
Match: slit3 (slit guidance ligand 3 [Source:NCBI gene;Acc:103037708]) HSP 1 Score: 677.552 bits (1747), Expect = 0.000e+0 Identity = 471/1553 (30.33%), Postives = 734/1553 (47.26%), Query Frame = 1 Query: 157 CPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQLQCINDNIFNKLGMLKYLLLEGNKIFWIS-EAISKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCASEDKANLENMCPSDKKVCSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQIYNFQC--NKEFESKYETHDEECVPKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLPPIYSIHEAPNLMKLDLSFNQIETLEPDVFTRS------------------------SKLREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQFHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCDG---NA---SVYHSVDSCHETNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVLSFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICK-NIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYC-NDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNSIYF-QKNSYIEISPPSRGFLMPRGNISLNFMTNSSSGLILYQGSEQTYLVVNLYQGHLRISYNLGDKVVGPEGYSMGMVNDSRLHALHIEIVGENLTMYLNGRVHCTLKSKYLNSNRYMALDTGIFIGGAPHKILQSAIMKFHIETDSEFIGCMESLLINRKKIDFSKYLPKSINVHPGCGKLISTV---------VASDLKIPSIMXXXXXXXXXXXXXXXNNVCKRPGICKNQGVCEVLPGSKKKFRCICKNGFVGRKCKKRKS--MCHGTFTESH--IVDPDSQGTCKSIERFQYRICTG--TCSTSRMQSDVIKNFYHK-------------PIDPSSSRCCQPTKYSTHEIQFKCENGRVYRRAANLPKNCKC 4623 CP C C VEC+ + F +VP + RL+L N+I+VI + F L L+ L+L+ NR+ + F + L +L L+ N IQ +P F +T ++ L L N + CI D F L L+ L L N I I + + + L+ LR+H N L+C+CHL+WL+ +L+Q+ + AH C +P + + + + +F C ++ + C VC C+C+++ IV C K L+ IP +LP +VE+ L+ N I + G S YK LK+I L++N + DI AF GL LTS+ L N + I K F GLVSL L L N I CL +T + + L+LL L +NKL ++ K F P ++ L+L NP CDC + W+ DY+ D T A C PR L + ++ F+C ++++ S+ EC + CP +C C VV+CSN L S+P + T L ++ N + L + + PNL K++LS N++ + VF + + L+ + L SN++ C++N TF L + +L L +N IS I +F +L + L N + CDC L W+ W + V GN P CQ P LK+ PI + F CDG NA SV D C +++V V CS+ L+ +P IPKDTTELYL+ N L S+ + +L L + LS N I+ L + + + L TL+LS+N+++C+ AF L +L++L L ND+STIP AFN L +L+++ALG NP +CDC ++WL+Q+ F + GI+ C P M + + + F C D + +KC C+ PC++NG C +Y C CP+ + G+NC+ NAC QPC NGG C G++ CVCP GF G +C +N +DC N C N +TC D N+Y+C C +RG C+ C +PCQ+ +C L Y+C C G+ G+ CE++ +DC N C+H ++CVD N Y C+C+ G+ G FCE P P +L + S C +C N C+ + + C+C PG+ G+ CQ +++++F K S+I++ R ++ +ISL T+ +G++LY+ + L + LYQGH+R+ Y++ K YS+ VND H + + I + L++ ++ +L L + +T ++IGG P ++ + F GC+ ++ IN + + L + PGC V + P+ T+ N+ C G C G+ + C C G+ G C R+ C G VD + TC + C +C ++ + ++ HK P CC TK +I FKC +G + +C C Sbjct: 33 CPAKCSCSGSHVECQQQGFRSVPRGIPRNAERLHLENNQISVIERGAFQDLKQLERLRLNRNRLQVLPELLFQSTPKLGRLDLSENQIQAIPRKSFRGITNVKNLQLDSNHISCIEDGAFRALRDLEILTLNNNNITLIPLSSFNHMPKLRTLRLHSNNLHCDCHLSWLSDWLRQRKGL----AHFTQCMSPAHMRGLSVPDVQKRDFVCTGPAQSEPRS-CAPQASVCPASCSCTNN-IVDCRRKGLTEIPANLPEGIVEIRLEQNLIKSIPPGAFSPYKKLKRIDLSKNHISDIADDAFSGLRSLTSLVLYGNKIAEIPKGLFDGLVSLQLLLLNANKINCLRVNTFKDLQSLNLLSLYDNKLQTISKGLFTPLRAIKTLHLAQNPFMCDCHLKWLADYLFDNPIETSGARCSHPRRLANKRISQIKGKKFRCTGSEDYRSRLTG---ECFQDLVCPERCRCEGTVVDCSNLKLTSIPPHLPEHTTDLRLNDNEISSLDATGAFKKLPNLRKINLSNNKLRDIREGVFDGAGGVLELLLTGNKLQAVQGRMFRGLTGLKTLMLRSNQITCVDNATFTGLSSVRLLSLYDNRISTIAPGAFNTLHSLSTINLLSNPYVCDCHLAWLGLWLKKTRVVSGN---PRCQRPAFLKEIPIQDVAAPDFTCDGVEENACLPSVPRCPDPCTCSDTV---------------------------VRCSNHGLRSLPKGIPKDTTELYLEGNMLTSVPK-ELSSLKQLSLVDLSNNSISSLTPFIFSNMTQLSTLILSYNQIRCVPVHAFDGLRSLRLLTLHGNDLSTIPEGAFNHLTSLSHLALGANPLYCDCELRWLSQWVKAGFKEPGIARCTGPPDMADRLLLTTPLNRFQCKGPV-DLNLMSKCAPCLATPCQNNGTCVSDVTGSYHCTCPFGYKGRNCEIAANACLSQPCINGGTCHLTPGQEGHFSCVCPPGFEGQRCEVNPNDCEDNDCENNSTCVDGINNYTCVCPPNYRGDLCEEVVDACLPGFDPCQHDSKCVPL-TRGYRCECLPGYVGQHCEQDYNDCLENKCQHGAECVDAINGYTCVCKDGFSGLFCENPP--PMIL----------------------LQKTSPCDQSDCQNGAECVLME----GEPVCRCLPGFFGNKCQKMHTVHFLGKESFIQL---PRTKILSSTHISLQVATDKDNGMLLYK-EDHDPLAIELYQGHIRLMYDIA-KYPPTTVYSVESVNDGFFHTVELLIQNQTLSLVVDKGAPKSLGK--LPRPPTVETNTRLYIGGVPSELSSPSSRSGADRQSQSFNGCIHNVRINGELQELGSGLDSVKAILPGCHACSVCAQGACRQRDDVGVTCECPAGSTGPLCDQQGTSNPCQNSRCVH-GQCVANGL---------AYSCKCAQGYSGPYCATREEPLACRGLHCVHGECRVDESGEATCACQPGYTGPTCETELSCQGEMIREQLKRHMGHKTCTSTIKVPRMECPRVCHPGLCCTSTKSRRRKITFKCSDGFTFSEELETTLDCNC 1498 HSP 2 Score: 282.722 bits (722), Expect = 9.844e-77 Identity = 207/653 (31.70%), Postives = 317/653 (48.55%), Query Frame = 1 Query: 139 SDESEICPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQLQCINDNIFNKLGMLKYLLLEGNKIFWISEAI-SKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQC-ASED-KANLENMCPSDKKVCSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQ-GYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQIYNFQCNKEFESKYETHDEECVPKVS-CPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLPPIYSIHEAPNLMKLDLSFNQIETLEPDVFTRSSKLREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQFHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCDG 2082 + ++ +CP C C N V+C+ + +P L E + L N I I F LK + LS N I+ I AF L +L L L N I +P LF L LQ L L N++ C+ N F L L L L NK+ IS+ + + L+ +K L + +N C+CHL WL +L I GA CS P RL++ IS + +F+C SED ++ L C D VC ++C C +V C +L++IP LP + +L L DN I+ L G K L+KI L+ N + DI G F G + + L N L+ ++ F GL L L L+ N I C+ T + LL L +N+++++ F +L + L+ NP CDC ++W+ ++K + C +P L+ P++++ +F C+ + C+P V CP CTCS VV CSN GL SLP+ I T L + N L +P + L +DLS N I +L P +F+ ++L + L+ N+++C+ F L+ L +L L N +S I +F + +L +L L N +CDC+L+W+S+W + + G R C P + D + F C G Sbjct: 250 APQASVCPASCSCTNNIVDCRRKGLTEIPANLPEGIVEIRLEQNLIKSIPPGAFSPYKKLKRIDLSKNHISDIADDAFSGLRSLTSLVLYGNKIAEIPKGLFDGLVSLQLLLLNANKINCLRVNTFKDLQSLNLLSLYDNKLQTISKGLFTPLRAIKTLHLAQNPFMCDCHLKWLADYLFDNP-IETSGAR---CSHPRRLANKRISQIKGKKFRCTGSEDYRSRLTGECFQD-LVCPERCRC-EGTVVDCSNLKLTSIPPHLPEHTTDLRLNDNEISSLDATGAFKKLPNLRKINLSNNKLRDIREGVFDGAGGVLELLLTGNKLQAVQGRMFRGLTGLKTLMLRSNQITCVDNATFTGLSSVRLLSLYDNRISTIAPGAFNTLHSLSTINLLSNPYVCDCHLAWLGLWLKKTRVVSGNPRCQRPAFLKEIPIQDVAAPDFTCDG-------VEENACLPSVPRCPDPCTCSDTVVRCSNHGLRSLPKGIPKDTTELYLEGNMLTSVP--KELSSLKQLSLVDLSNNSISSLTPFIFSNMTQLSTLILSYNQIRCVPVHAFDGLRSLRLLTLHGNDLSTIPEGAFNHLTSLSHLALGANPLYCDCELRWLSQWVKAGFKEPGIAR---CTGPPDMADRLLLTTPLNRFQCKG 884
BLAST of slit1 vs. Ensembl Cavefish
Match: slit1a (slit homolog 1a (Drosophila) [Source:ZFIN;Acc:ZDB-GENE-030722-3]) HSP 1 Score: 633.254 bits (1632), Expect = 0.000e+0 Identity = 450/1571 (28.64%), Postives = 715/1571 (45.51%), Query Frame = 1 Query: 148 SEICPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQLQCINDNIFNKLGMLKYLLLEGNKIFWIS-EAISKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCASEDKANLENMCPSDKKVCSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQIYNFQCNKEFESKYETHDEECVPKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLPPIYSIHEAPNLMKLDLSFNQIETLEPDVFTRSSKLREISLNSNRLKCINNETFKNLKEL---------------------------------------------EILQLSENAISCIVTDSFXXXXXXXXXXXXXXQFHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCDGNASVYHSVDSCHETNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVLSFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYC-DIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNSIYF-QKNSYIEISPPSRGFLMPRGNISLNFMTNSSSGLILYQGSEQTYLVVNLYQGHLRISYNLGDKVVGPEGYSMGMVNDSRLHALHIEIVGENLTMYLNGRVHCTLKSKYLNSNRYMALDTGIFIGGAPHKILQSAIMKFHIETDSEFIGCMESLLINRKKIDFSKYLPKSINVHPGCGKLISTVVASDLKIPSIMXXXXXXXXXXXXXXXNNVCKRPGICKNQGV----CEVLPGSKKKFRCICKNGFVGRKCKK--------RKSMC-HGTFTESHIVDP-------------DSQGTCKS---IERFQYRICTGTCSTSRMQSDVIKNFYHKPIDPSSSRCCQPTKYSTHEIQFKCENGRVYRRAANLPKNCKCVA 4629 S CP C C +V+ L++ L+ N+I I + F + L+ L+L+ N++ + F L +L L+ N IQ +P F T ++ L L N + CI D F L L+ L L N I I + + + L+ R+H N LNC+CHL+WL+ +L+Q+ + CS+P +L I ++ + +EF C+ + + C C C CS++ IV C GK L+ IP +LP N+ E+ L+ N I + G S YK L++I L+ N + +I P AF GL L S+ L N + + K F GL +L L L N I C+ +T + + L LL L +NK+ +L K TF ++ L+L NP CDC + W+ DY++ T A C PR L + ++ F+C+ +++ + EC CP KC C S+VV+CSN L +PE I ST L +++N + L + L K++LS N+I +E VF +S + E+ L +N+L + + FK L+ L +L L +N ++ I +F L L L N F+CDC+L W+ +W R+ V GN P CQ P LK+ P + +AS E ++T V CS+ L+ +P IP++ TELYLD N S+ L+ + LS N I L + L TL+LS+N L+CI AF L +L++L L ND+S +P F+D+ +L+++A+G NP +CDC ++WL+ + + + GI+ C P M+ K + + F C + A I AKC+ C+ PC ++G C Y+C C F GK+C+ +NAC PC NGG+C+ + C C +GF GP C IN+DDC N C NGATC D N+Y+C C + G+ C +++ + +PC++ C L+ KCVC+ G+ G C + DDC+ + C++ +CVD N Y C C GY G+ CE+P S+C +C N+ C+ + ++ C+C PG+ G C+ L S+ F ++SY+ +S P+ NI+L T +G++LY G + ++ V LYQGH+++SY+ G + G YS +ND + H + + + + + ++G + T+ S R + + +++GG P + SA+ + I+ S F GC+++L IN + DF+K K V PGC + P C +P QG + +P + +RC C+ G+ G C + R+ C HG S D D++ C+ + +Q + C T+RM S + + CC + + F+C +G + C CV Sbjct: 38 SSACPALCTCSGTTVDLNLSFL----AFLEQ------LMENQITAIERGAFDDMKELERLRLNRNQLHQLPELLFQKNAALSRLDLSENFIQAIPRKAFRGATDIKNLQLDKNHIGCIEDGAFRALRGLEVLTLNNNNISGIPVSSFNHMPKLRTFRLHSNSLNCDCHLSWLSQWLRQRPTL----GLFTQCSSPAQLRGINVAEIQKHEFSCSGHAENSNPPSCNLAAGSCPAMCTCSNN-IVDCRGKGLTAIPANLPDNMAEIRLEQNGIKSVPPGAFSPYKKLRRIDLSNNQISEIAPDAFQGLRSLNSLVLYGNKITELPKGVFDGLYALQLLLLNANKIHCVRANTFQDLQNLSLLSLYDNKIQTLAKGTFASLRAIQTLHLAQNPFICDCNLKWLADYLRSNPIETSGARCASPRRLANKRIGQIKSKKFRCSGTEDTRL---NNECNNDPVCPAKCRCESNVVDCSNLRLTKIPEHIPASTTELRLNNNEITSLEATGAFKTLTQLKKINLSNNKISEIEDGVFEGASSVNELHLTANQLDSVRSSMFKGLEGLRMLNQFSGQELTKILHYVFFLSPECSATTASAASTTAASLGLYNVRLLSLYDNQLTTITPGAFDTLQTLSTLNLLANSFNCDCRLAWLGDWLRSRKIVTGN---PRCQRPAFLKEIPAAGRGQE------DASCMPRPQCPSECTCLET------------------------VVRCSNKHLRALPKGIPRNVTELYLDGNQF-SMVPKELAAFKYLQLVDLSNNKINSLTNASFANMSQLTTLILSYNSLRCIPSLAFSGLRSLRLLSLHGNDISELPDGIFSDVMSLSHLAIGANPLYCDCRLRWLSDWVKTGYKEPGIARCTGPPGMEGKLLLTTPAKKFECQGNVDPA-ILAKCNPCLSSPCLNHGSCHTDPVEVYKCTCAEGFKGKHCETALNACVSNPCANGGVCQVTGEGDAFSCSCSLGFEGPSCEINIDDCEDNDCENGATCIDGVNNYTCLCSPYYTGEMCEEMEDMCAPGHSPCKHQSTC-LITATGPKCVCAPGYVGDDCSVDYDDCQEHKCQNNGECVDEINGYSCSCTRGYSGQLCEVPP-----------------------------APRSLCELADCQNDAPCV----ERAGRALCQCLPGFGGPRCEKLVSVNFVDRDSYLLLSDLKN---WPQANITLQVSTAEDNGILLYNG-DNDHIAVELYQGHVKVSYDPGSQ-PGHAIYSTETINDGQFHTVELVTFDQMVNLSIDGGLPTTMDS--FGKLRPLNGEAPLYVGGMPLDVPSSALRMWQIQNGSSFHGCIQNLYINNELQDFTKTQMKP-GVVPGCEPCRKIYCLHGICQPDAAAGPVCHCQPGWSGPH---CDQPATSPCQGSKCVHGKCVPLDTQTYRCECQEGYRGALCNQQGELFNPCRRLQCKHGRCQVSDTGDAYCHCESGYTGDLCDAESECRGEPVRDFYQVQRGYAICQTTRMVS-----WVECSGACDTGACCASQRMKRRKYTFECSDGTSFSEEVEKTIKCGCVG 1505 HSP 2 Score: 149.443 bits (376), Expect = 1.205e-35 Identity = 126/467 (26.98%), Postives = 208/467 (44.54%), Query Frame = 1 Query: 127 INDPSDESEICPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLK-LNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLD-LRDNQLQCINDNIF------------------NKLGM--LKYLLLEGNKIFWISE-AISKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCASEDKANLENMCPSDKKVCSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQIYNFQC 1458 +N+ + +CP C+CE+ V+C + +P + TT L L N I + + K L LK + LSNN+I+ IE G F+ ++ +L L N + + +F L L+ L+ +L I +F LG+ ++ L L N++ I+ A L+ L L + N NC+C LAWL +L+ + + + C P L IP + + C + CPS+ C C + +V+C K L +P+ +P NV EL L N +++ + L+ +K+L+ + L+ N + + +F ++QLT++ L+ N+L+ I AF GL SL L L NDI LP F ++L +L + NPL CDCR+ W+ D++K +A C P ++G L F+C Sbjct: 468 LNNECNNDPVCPAKCRCESNVVDCSNLRLTKIPEHIPASTTELRLNNNEITSLEATGAFKTLTQLKKINLSNNKISEIEDGVFEGASSVNELHLTANQLDSVRSSMFKGLEGLRMLNQFSGQELTKILHYVFFLSPECSATTASAASTTAASLGLYNVRLLSLYDNQLTTITPGAFDTLQTLSTLNLLANSFNCDCRLAWLGDWLRSRKIVTG----NPRCQRPAFLKEIPAAGRGQEDASCMPRPQ------CPSE-------CTC-LETVVRCSNKHLRALPKGIPRNVTELYLDGNQFSMVPK-ELAAFKYLQLVDLSNNKINSLTNASFANMSQLTTLILSYNSLRCIPSLAFSGLRSLRLLSLHGNDISELPDG------------------------IFSDVMSLSHLAIGANPLYCDCRLRWLSDWVKTGYKEPGIARCTGPPGMEGKLLLTTPAKKFEC 891
BLAST of slit1 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000003392.1 (pep scaffold:Pmarinus_7.0:GL476560:299294:319002:1 gene:ENSPMAG00000003104.1 transcript:ENSPMAT00000003392.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 217.624 bits (553), Expect = 1.629e-59 Identity = 183/725 (25.24%), Postives = 288/725 (39.72%), Query Frame = 1 Query: 2281 YLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVLSFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATC----NDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSG--------QCQLLPNNDYKCV-CSKGWE--------------GKQCERNTDD----CKYNSCKH-----ESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNSIYF-QKNSYIEISPPSRGFLMPRGNISLNFMTNSSSGLILYQGSEQTYLVVNLYQGHLRISYNLGDKVVGPEGYSMGMVNDSRLHALHIEIVGENLTMYLNGRVHCTLKSKYLNSNRYMALDTGIFIGGAPHKILQSAIMKFHIETDSEFIGCMESLLINRKKIDFSKYLPKSINVHPGCGKLISTVVASDLKIPSIMXXXXXXXXXXXXXXXNNVCKRPGICKNQGVCEVLPGSKKKFRCICKNGFVGRKCKKRKS 4344 YLD N L ++ N +L + LS N I+ + + + L TL+LS+N+L+C+ AF L L++L L NDVS IP+ F+DL +L+++ALG NP HCDC ++WL+ + + + GI+ C+ P M K + + F C D I AKC N C QPC N G+C + D Y C C GF+G C ++ CV + C N TC + ++ + + R C I L C + + QLL V W G Q + ++D C + SC+ + L C C G CE+P M Y + S C YH+C N G C+ + C+C G+ G C L S+ F Q++SY+++ PP +G P+ NISL T+ +G++LY G + ++ V +Y+G +R+SY+ G P YS+ +ND H + I + + + + ++G T+ L + L+ ++IGG P + +A+ + H F GC+ L IN + D + PGC C++ G C + G C C C+ G+ G +C +R Sbjct: 1 YLDGNQL-TMVPPELSNYKHLSFIDLSNNHISTIANFTFSNITQLSTLILSYNRLRCLPLRAFAGLKTLRLLSLHGNDVSMIPHGVFSDLTSLSHLALGSNPLHCDCAMRWLSDWVKVDYKEPGIARCSGPGDMAEKLLLTTPSKKFECVGP-PDVSIQAKC---------------------------------------NPCLSQPCLNHGVCSS-DPVDFYRCTCSYGFKGQDCGTPINACVSSPCQNAGTCHVREDGDDSGFQLYRRPDVRAGNCQINVLNCPNFLDFKRKTVEFNSTVVMAQLLTTTKTSQVHLHHSWHSPPPHPFSLPTRFGGPQSDWSSDSYCAICYFLSCESIVYPTGTDVTSLRTTNTCPCPTPGSGPLCEVPP------------------PMVY-------LQTSACQYHDCQNGGQCVE----AGGEPECQCLQGFVGSRCDKLTSVNFVQRDSYLQL-PPIKG--QPKANISLRVATDEDNGMLLYNGVSE-HIAVEIYRGRVRLSYDAG-SYPNPTIYSLETINDGEFHTVEIVTLEQMVNLSIDGGSPKTMIK--LGKPASLTLELPLYIGGMPTDVNSAALKQMHDRNGMGFHGCIRDLYINDELQDLRAPGRPPAGILPGCAP----------------------------------CRQQGACVH-GACRA--AGPSGHTCECEAGWTGAQCDQRSG 610 HSP 2 Score: 76.6406 bits (187), Expect = 3.069e-14 Identity = 48/163 (29.45%), Postives = 72/163 (44.17%), Query Frame = 1 Query: 1069 QLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQIYNFQCNKEFESKYETHDEECVPKVSCPLKCTCSSDVVN 1557 L+ I L+ N++ TI F + L L L YN + CLP K L LL L N ++ + F +L +L L NPL+CDC M W+ D++K +A C P ++ L F+C + + C+ + C CSSD V+ Sbjct: 19 HLSFIDLSNNHISTIANFTFSNITQLSTLILSYNRLRCLPLRAFAGLKTLRLLSLHGNDVSMIPHGVFSDLTSLSHLALGSNPLHCDCAMRWLSDWVKVDYKEPGIARCSGPGDMAEKLLLTTPSKKFECVGPPDVSIQAKCNPCLSQ-PCLNHGVCSSDPVD 180 HSP 3 Score: 72.0182 bits (175), Expect = 8.448e-13 Identity = 50/158 (31.65%), Postives = 77/158 (48.73%), Query Frame = 1 Query: 1621 MSHNNLKKLPPIYSIHEAPNLMKLDLSFNQIETLEPDVFTRSSKLREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQFHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCDGNASV 2094 + N L +PP S ++ +L +DLS N I T+ F+ ++L + L+ NRL+C+ F LK L +L L N +S I F + +L +L L N HCDC ++W+S+W + Y G R C P + + + K F C G V Sbjct: 2 LDGNQLTMVPPELSNYK--HLSFIDLSNNHISTIANFTFSNITQLSTLILSYNRLRCLPLRAFAGLKTLRLLSLHGNDVSMIPHGVFSDLTSLSHLALGSNPLHCDCAMRWLSDWVKVDYKEPGIAR---CSGPGDMAEKLLLTTPSKKFECVGPPDV 154 HSP 4 Score: 61.6178 bits (148), Expect = 1.375e-9 Identity = 52/207 (25.12%), Postives = 90/207 (43.48%), Query Frame = 1 Query: 259 LLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQLQCINDNIFNKLGMLKYLLLEGNKIFWISEAI-SKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCASEDKANLENMC-PSDKKVCSKKCNCSHDGI 873 L GN++ ++ + +L + LSNN I++I + FS +T+L L L N+L+C+ F L L+ L L GN + I + S L L L + N L+C+C + WL+ ++ K + +PG CS P ++ + + S +F+C +++ C P + C CS D + Sbjct: 2 LDGNQLTMV-PPELSNYKHLSFIDLSNNHISTIA------------------------NFTFSNITQLSTLILSYNRLRCLPLRAFAGLKTLRLLSLHGNDVSMIPHGVFSDLTSLSHLALGSNPLHCDCAMRWLSDWV--KVDYKEPGI--ARCSGPGDMAEKLLLTTPSKKFECVGPPDVSIQAKCNPCLSQPCLNHGVCSSDPV 179 HSP 5 Score: 55.0694 bits (131), Expect = 1.401e-7 Identity = 39/119 (32.77%), Postives = 52/119 (43.70%), Query Frame = 1 Query: 2902 PCRNGGICKN----IDNYGNYECVCPVGFRGPKCTINVDD-CVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQN----SGQCQLLPNNDYKCVCSKGWEGKQCER 3231 PCR G C + + C C G+ G +C D C N CV+GA SYSC+C +GF G CD + NPC+ G C++ C CS G+ G C+R Sbjct: 572 PCRQQGACVHGACRAAGPSGHTCECEAGWTGAQCDQRSGDPCQANKCVHGACIALDAFSYSCRCANGFVGTLCDEEQR--ELFNPCRGVRCKHGTCRISGAGKPFCECSPGYTGDNCDR 688 HSP 6 Score: 51.6026 bits (122), Expect = 1.611e-6 Identity = 33/114 (28.95%), Postives = 52/114 (45.61%), Query Frame = 1 Query: 3025 CVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNS----GQCQLLPNNDYKCVCSKGWEGKQCERNTDD----CKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCE 3342 CV+GA + ++C+C++G+ G CD + +PCQ + G C L Y C C+ G+ G C+ + C+ CKH + + C C PGY G C+ Sbjct: 579 CVHGACRAAGPSGHTCECEAGWTGAQCDQR-----SGDPCQANKCVHGACIALDAFSYSCRCANGFVGTLCDEEQRELFNPCRGVRCKHGTCRISGAGKPFCECSPGYTGDNCD 687 HSP 7 Score: 49.2914 bits (116), Expect = 8.530e-6 Identity = 38/138 (27.54%), Postives = 55/138 (39.86%), Query Frame = 1 Query: 3154 GQCQLLPNNDYKCVCSKGWEGKQC-ERNTDDCKYNSCKHESQCVDLHN-DYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLC 3561 G C+ + + C C GW G QC +R+ D C+ N C H + C+ L Y C C G++G C D Q E+ + C C + I+G K +C+C PGY+G C Sbjct: 582 GACRAAGPSGHTCECEAGWTGAQCDQRSGDPCQANKCVHGA-CIALDAFSYSCRCANGFVGTLC-----------------DEEQRELF-----------NPCRGVRCKHGTCRISG----AGKPFCECSPGYTGDNC 686
BLAST of slit1 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000002643.1 (pep scaffold:Pmarinus_7.0:GL478752:33855:40482:1 gene:ENSPMAG00000002413.1 transcript:ENSPMAT00000002643.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 130.183 bits (326), Expect = 4.952e-33 Identity = 78/247 (31.58%), Postives = 115/247 (46.56%), Query Frame = 1 Query: 2773 CMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMC-RPGYIGKFCEIP----AWKPTVLKTLISRRDINQSEMTYENLENEVISESV--CAYHECLNNGICI 3492 C PC H G C + C C + G NC+ + C PC N +C +D G Y+C C GF+G C I++++C C++G TC + + YSC C GF G+ C + C PC N C L + ++CVC +G+ G CE D+C+ C++ C+DL + Y C C GY GK CE+ A P + +N + + + + E V C C+N G CI Sbjct: 1 CASGPCLHGGTCLPAADGGFSCRCAPGYSGDNCEASVGGCASGPCLNDAVC--VDEPGGYKCYCVPGFQGRHCEIDINECASEPCLHGGTCTNGRDRYSCACPDGFAGERCQLDTNECAS-QPCHNGATCVDL-DGKFECVCPRGYTGAMCETEVDECESGPCQNGGVCIDLVDSYTCDCLETGYEGKDCELEILECASNPCRHNSTCIEGILNFTCICWPGFAGDACEEDVNECGDEPCMNGGQCI 243 HSP 2 Score: 129.798 bits (325), Expect = 6.161e-33 Identity = 88/264 (33.33%), Postives = 131/264 (49.62%), Query Frame = 1 Query: 2773 CMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKC-QSGFRGKYCDIKFLYCNDINPCQNSGQC--QLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSY-CKCKPGYSG 3552 C PC H G C+ Y C CP F G+ C N C QPC NG C ++D G +ECVCP G+ G C VD+C C NG C DL +SY+C C ++G+ GK C+++ L C NPC+++ C +L ++ C+C G+ G CE + ++C C + QC++L +P + GK E+P + + R + + EN ++ C CLN G CI+ N S+ C+C PG++G Sbjct: 78 CASEPCLHGGTCT-NGRDRYSCACPDGFAGERCQLDTNECASQPCHNGATCVDLD--GKFECVCPRGYTGAMCETEVDECESGPCQNGGVCIDLVDSYTCDCLETGYEGKDCELEILECAS-NPCRHNSTCIEGIL---NFTCICWPGFAGDACEEDVNECGDEPCMNGGQCIELS-------KPDFYGKLSELPGTFHYANASGFTCRCLPGT-----TGENCSVNLDECESQPCLNGGSCID-----NIGSFSCECAPGFTG 317 HSP 3 Score: 71.633 bits (174), Expect = 2.202e-13 Identity = 58/206 (28.16%), Postives = 89/206 (43.20%), Query Frame = 1 Query: 2722 ICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDC-PYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDI--KFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIG 3330 +C A + D C PC++ G C + +Y CDC + GK+C+ I C PCR+ C I+ N+ C+C GF G C +V++C C+NG C +L+ + F GK ++ F Y N + + C C G G+ C N D+C+ C + C+D + C C PG+ G Sbjct: 137 VCPRGYTGAMCETEVDECESGPCQNGGVCIDLV-DSYTCDCLETGYEGKDCELEILECASNPCRHNSTC--IEGILNFTCICWPGFAGDACEEDVNECGDEPCMNGGQCIELS-------KPDFYGKLSELPGTFHYAN---------------ASGFTCRCLPGTTGENCSVNLDECESQPCLNGGSCIDNIGSFSCECAPGFTG 317 HSP 4 Score: 64.3142 bits (155), Expect = 6.192e-11 Identity = 42/143 (29.37%), Postives = 58/143 (40.56%), Query Frame = 1 Query: 3136 NPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQ 3564 PC + G C + + C C+ G+ G CE + C C +++ CVD YKC C PG+ G+ CEI DIN+ CA CL+ G C NG + C C G++G CQ Sbjct: 4 GPCLHGGTCLPAADGGFSCRCAPGYSGDNCEASVGGCASGPCLNDAVCVDEPGGYKCYCVPGFQGRHCEI---------------DINE-----------------CASEPCLHGGTCTNG----RDRYSCACPDGFAGERCQ 110
BLAST of slit1 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000002014.1 (pep scaffold:Pmarinus_7.0:GL482490:2146:12219:-1 gene:ENSPMAG00000001825.1 transcript:ENSPMAT00000002014.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 123.635 bits (309), Expect = 4.894e-32 Identity = 65/195 (33.33%), Postives = 102/195 (52.31%), Query Frame = 1 Query: 2758 AKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCE 3342 A D C PC++ G+C + +Y CDC F GK C+ I+ C PC+NGG C ++ G++ C C G+ G C ++D+C + C NG C + S++C C +G+ G CD C +PC N G+C+ + C C+ G+ G +CE + ++C + C + +C D + C C PG+ G CE Sbjct: 10 ADVDECSPNPCQNGGRC-IDGAASYTCDCAQGFTGKRCETDIDECTSDPCQNGGQC--VNGAGSFTCSCLAGYTGTLCDSDIDECTSDPCQNGGQCVNGAGSFTCSCLAGYTGTLCDSDVNECAS-SPCLNGGRCR-DGVAAFTCECAPGFTGTRCETDVNECASSPCLNGGRCRDGVAAFTCECAPGFTGTRCE 199 HSP 2 Score: 111.309 bits (277), Expect = 1.018e-27 Identity = 69/228 (30.26%), Postives = 103/228 (45.18%), Query Frame = 1 Query: 2881 INACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQ 3564 ++ C PC+NGG C ID +Y C C GF G +C ++D+C + C NG C + S++C C +G+ G CD C +PCQN GQC + + C C G+ G C+ + ++C + C + +C D + C C PG+ G CE D+N+ CA CLN G C +G C+C PG++G C+ Sbjct: 12 VDECSPNPCQNGGRC--IDGAASYTCDCAQGFTGKRCETDIDECTSDPCQNGGQCVNGAGSFTCSCLAGYTGTLCDSDIDECTS-DPCQNGGQC-VNGAGSFTCSCLAGYTGTLCDSDVNECASSPCLNGGRCRDGVAAFTCECAPGFTGTRCET---------------DVNE-----------------CASSPCLNGGRCRDG----VAAFTCECAPGFTGTRCE 199 HSP 3 Score: 94.3597 bits (233), Expect = 6.258e-22 Identity = 51/160 (31.87%), Postives = 83/160 (51.88%), Query Frame = 1 Query: 2767 DSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDC 3246 D C PC++ G+C + ++ C C + G CD I+ C PC+NGG C ++ G++ C C G+ G C +V++C + C+NG C D +++C+C GF G C+ C +PC N G+C+ + C C+ G+ G +CE + C Sbjct: 51 DECTSDPCQNGGQC-VNGAGSFTCSCLAGYTGTLCDSDIDECTSDPCQNGGQC--VNGAGSFTCSCLAGYTGTLCDSDVNECASSPCLNGGRCRDGVAAFTCECAPGFTGTRCETDVNECAS-SPCLNGGRCRD-GVAAFTCECAPGFTGTRCETVLNPC 205
BLAST of slit1 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000005361.1 (pep scaffold:Pmarinus_7.0:GL479377:6554:13965:1 gene:ENSPMAG00000004883.1 transcript:ENSPMAT00000005361.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 118.627 bits (296), Expect = 5.069e-30 Identity = 72/196 (36.73%), Postives = 108/196 (55.10%), Query Frame = 1 Query: 2758 AKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEI 3345 A + C+ PC++NG C L ++ + C C F G C+ ++ C +PCR G C N D G++ C C +GF GP CT NV+DC N C++G C D N++ C C+ G+ G CD C N C+N C L + C C+ G+ G+ C+ N DDC N C +++ C+D N Y C+C G+ G++C + Sbjct: 3 ADVNECLSNPCQNNGIC-LNRNNKFLCQCSSGFTGTFCEIDVDECNSRPCRQWGTCVNTD--GSFYCSCVLGFNGPLCTNNVNDCAPNPCIHG-RCVDQVNAFFCSCEEGWSGTTCDTNIDDCVG-NLCRNGATCVDLVK-AFNCSCAAGFTGQYCQNNIDDCIGNQCTNQAICIDGINRYSCICPDGWSGEYCAV 192 HSP 2 Score: 107.071 bits (266), Expect = 4.255e-26 Identity = 73/205 (35.61%), Postives = 98/205 (47.80%), Query Frame = 1 Query: 2725 CSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKY--NSCKHE-SQCVDLHNDYKCMCRPGYIG 3330 CS + D C RPC+ G C + T ++ C C F+G C +N C PC +G + +D + C C G+ G C N+DDCVGNLC NGATC DL +++C C +GF G+YC C N C N C + N Y C+C GW G+ C + D+C +SC C + Y C C GY G Sbjct: 30 CSSGFTGTFCEIDVDECNSRPCRQWGTC-VNTDGSFYCSCVLGFNGPLCTNNVNDCAPNPCIHG---RCVDQVNAFFCSCEEGWSGTTCDTNIDDCVGNLCRNGATCVDLVKAFNCSCAAGFTGQYCQNNIDDCIG-NQCTNQAIC-IDGINRYSCICPDGWSGEYCAVDIDECSTGRSSCHTTLGVCSNSPGSYSCACIAGYEG 228 HSP 3 Score: 93.2041 bits (230), Expect = 2.567e-21 Identity = 73/231 (31.60%), Postives = 104/231 (45.02%), Query Frame = 1 Query: 2869 CDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLC 3561 C +N C PC+N GIC N +N + C C GF G C I+VD+C C TC + + S+ C C GF G C C NPC + G+C + N + C C +GW G C+ N DDC N C++ + CVDL + C C G+ G++C+ N+++ C ++C N ICI+G + C C G+SG C Sbjct: 1 CFADVNECLSNPCQNNGICLNRNN--KFLCQCSSGFTGTFCEIDVDECNSRPCRQWGTCVNTDGSFYCSCVLGFNGPLCTNNVNDCAP-NPCIH-GRC-VDQVNAFFCSCEEGWSGTTCDTNIDDCVGNLCRNGATCVDLVKAFNCSCAAGFTGQYCQ-------------------------NNIDD-------CIGNQCTNQAICIDGI----NRYSCICPDGWSGEYC 190 HSP 4 Score: 67.0106 bits (162), Expect = 2.658e-12 Identity = 55/192 (28.65%), Postives = 83/192 (43.23%), Query Frame = 1 Query: 2989 CTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQ 3564 C +V++C+ N C N C + NN + C+C SGF G +C+I CN PC+ G C + + + C C G+ G C N +DC N C H +CVD N + C C G+ G C+ N+++ C + C N C++ + N C C G++G CQ Sbjct: 1 CFADVNECLSNPCQNNGICLNRNNKFLCQCSSGFTGTFCEIDVDECNS-RPCRQWGTC-VNTDGSFYCSCVLGFNGPLCTNNVNDCAPNPCIH-GRCVDQVNAFFCSCEEGWSGTTCDT-------------------------NIDD-------CVGNLCRNGATCVDLVKAFN----CSCAAGFTGQYCQ 153
BLAST of slit1 vs. Ensembl Sea Lamprey
Match: JAG1 (jagged canonical Notch ligand 1 [Source:HGNC Symbol;Acc:HGNC:6188]) HSP 1 Score: 123.25 bits (308), Expect = 2.270e-28 Identity = 73/221 (33.03%), Postives = 107/221 (48.42%), Query Frame = 1 Query: 2788 CKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVG-NLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDI--------------------------------------NPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGK 3333 C NG+C N+ C C F G C + +N C C NGG C +D +++C+CP G+ G C +VDDC N C+NG TC+DL N +SC+C++G++GK C K +C++ NPCQN C ++ + YKC+C GWEG C ++ +DC + C + CVD N Y+C C G+ G+ Sbjct: 465 CGPNGRCLSQAGGNFTCMCNSGFRGSFCHENVNDCAEGACHNGGTC--LDGINSFQCMCPDGWDGEFCENDVDDCSNHNPCLNGGTCHDLVNDFSCECKNGWKGKTCHSKISHCDEATCNNGGECFDVGDTFRCSCPTGWEGPTCNIAQSIACMPNPCQNGATC-VISGDSYKCICQSGWEGANCTQDINDCSSHPCYNGGTCVDGTNWYRCECAAGFAGE 682 HSP 2 Score: 121.709 bits (304), Expect = 5.793e-28 Identity = 88/298 (29.53%), Postives = 130/298 (43.62%), Query Frame = 1 Query: 2767 DSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCR--------------NGGICKNIDNY------------GNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIP---AWKP-------TVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSG 3552 D C+ PC++ +C + + +Y C C F G NC + C PC+ G+ GN+ C+C GFRG C NV+DC C NG TC D NS+ C C G+ G++C+ C++ NPC N G C L ND+ C C GW+GK C C +C + +C D+ + ++C C G+ G C I A P T + + S + I QS N ++ + C+ H C N G C++G C+C G++G Sbjct: 392 DLCVPNPCQNGARCVNLPNGDYFCHCSENFEGTNCTLLKDHCLTSPCQVIDSCTVAVPSNTSESGVAHVTSTVCGPNGRCLSQAGGNFTCMCNSGFRGSFCHENVNDCAEGACHNGGTCLDGINSFQCMCPDGWDGEFCENDVDDCSNHNPCLNGGTCHDLV-NDFSCECKNGWKGKTCHSKISHCDEATCNNGGECFDVGDTFRCSCPTGWEGPTCNIAQSIACMPNPCQNGATCVISGDSYKCICQSGWEGANCTQDI---NDCSSHPCYNGGTCVDG----TNWYRCECAAGFAG 681 HSP 3 Score: 117.857 bits (294), Expect = 9.454e-27 Identity = 77/209 (36.84%), Postives = 111/209 (53.11%), Query Frame = 1 Query: 2722 ICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHN-DYKCMCRPGYIGKFCEI 3345 +C+ A T D C PC + G C L + ++C CP + G C + C PC + CKN+ +G Y C C G+ G C I+V+DC G C+NG TC DL N YSC+C+ G+ G+ C+ + C +PC N GQC+ ++ Y CVC G+ G CE + D C N C++ ++CV+L N DY C C + G C + Sbjct: 226 LCAPGWTGANCTEDIDECASSPCGNGGTC-LNLVNGFRCTCPPQWTGHTCLVDTDECDDNPCVHARSCKNL--FGGYYCGCLPGWTGQNCDIDVNDCEGK-CLNGGTCKDLVNDYSCECKPGYTGRRCETEVNECAS-HPCLNGGQCRDAIDS-YYCVCMDGFAGDSCELDVDLCVPNPCQNGARCVNLPNGDYFCHCSENFEGTNCTL 428 HSP 4 Score: 110.923 bits (276), Expect = 1.269e-24 Identity = 63/172 (36.63%), Postives = 90/172 (52.33%), Query Frame = 1 Query: 2830 YQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEI 3345 Y+C C + G NC + I+ C PC NGG C N+ N + C CP + G C ++ D+C N CV+ +C +L Y C C G+ G+ CDI C C N G C+ L N DY C C G+ G++CE ++C + C + QC D + Y C+C G+ G CE+ Sbjct: 223 YKCLCAPGWTGANCTEDIDECASSPCGNGGTCLNLVN--GFRCTCPPQWTGHTCLVDTDECDDNPCVHARSCKNLFGGYYCGCLPGWTGQNCDIDVNDCE--GKCLNGGTCKDLVN-DYSCECKPGYTGRRCETEVNECASHPCLNGGQCRDAIDSYYCVCMDGFAGDSCEL 389 HSP 5 Score: 108.612 bits (270), Expect = 7.009e-24 Identity = 89/294 (30.27%), Postives = 121/294 (41.16%), Query Frame = 1 Query: 2767 DSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCV----------------------------DLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQ 3564 D C PC H C + Y C C + G+NCD +N C G+ C NGG CK++ N +Y C C G+ G +C V++C + C+NG C D +SY C C GF G C++ C NPCQN +C LPN DY C CS+ +EG C D C + C+ C ++ CMC G+ G FC +EN+ + CA C N G C++G N Q C C G+ G C+ Sbjct: 279 DECDDNPCVHARSCKNL-FGGYYCGCLPGWTGQNCDIDVNDCEGK-CLNGGTCKDLVN--DYSCECKPGYTGRRCETEVNECASHPCLNGGQCRDAIDSYYCVCMDGFAGDSCELDVDLCVP-NPCQNGARCVNLPNGDYFCHCSENFEGTNCTLLKDHCLTSPCQVIDSCTVAVPSNTSESGVAHVTSTVCGPNGRCLSQAGGNFTCMCNSGFRGSFC-------------------------HENVND-------CAEGACHNGGTCLDG-INSFQ---CMCPDGWDGEFCE 531 HSP 6 Score: 101.293 bits (251), Expect = 1.228e-21 Identity = 86/270 (31.85%), Postives = 116/270 (42.96%), Query Frame = 1 Query: 2764 CDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFG-QPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDD--CVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQ 3564 CD C+ P +G CS ++C C + G CDK +N C QPCRNGG C N +Y+C C G+ G C I+ + N C NG + + Y C C G+ G C C +PC N G C L N ++C C W G C +TD+C N C H C +L Y C C PG+ G+ C+I D+N E +CLN G C ++ C+CKPGY+G C+ Sbjct: 125 CDQCIPYPGCVHGGCS----KPWECRCDTNWGGLLCDKDLNYCGNHQPCRNGGSCSNA-QPDSYKCDCQKGYHGVNCEIDYVQHITLNNTCANGGSSVGSPSGYKCLCAPGWTGANCTEDIDECAS-SPCGNGGTCLNLVNG-FRCTCPPQWTGHTCLVDTDECDDNPCVHARSCKNLFGGYYCGCLPGWTGQNCDI---------------DVNDCEG------------------KCLNGGTC----KDLVNDYSCECKPGYTGRRCE 350
BLAST of slit1 vs. Ensembl Nematostella
Match: EDO48851 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RHH9]) HSP 1 Score: 170.244 bits (430), Expect = 2.149e-42 Identity = 108/310 (34.84%), Postives = 157/310 (50.65%), Query Frame = 1 Query: 2767 DSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDIN---------------QSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNSIYFQKNSYIEISPPSRGFLMPRGNI 3651 + C K PCK+ G C H Y C C F GKNC++ +N C PC+NGG+CKN +G Y C C GF G C +V++C N C NG C + + YSC C++GF GK C+ C+ NPC+N G C+ + Y C C G+ GK CE++ ++C N CK+ C + H Y C C+ G+ GK CE LKT+I + I ++ T +N E +V + C+ + C N G+C +N++ C CK G++G C+ S + SY ++ R PRGN+ Sbjct: 370 NECSKNPCKNGGVCKN-EHGGYSCACKAGFTGKNCEQDVNECSKNPCKNGGVCKN--EHGGYSCACKAGFTGKNCEQDVNECSKNPCQNGGVCKNEHGGYSCACKAGFMGKNCEQDVNECSK-NPCKNGGVCKN-EHGGYSCACKAGFTGKNCEQDVNECSKNPCKNGGVCKNEHGGYSCACKAGFTGKNCEQDG-----LKTIIHLQSIKCGVCKNEHGGYSCACKAGFTGKNCEQDV---NECSVNPCKNGGVC----KNEHGGYSCACKAGFTGKNCEQAPSKPRECRSYKKLDTADRAAGRPRGNV 662 HSP 2 Score: 156.762 bits (395), Expect = 2.892e-38 Identity = 97/275 (35.27%), Postives = 143/275 (52.00%), Query Frame = 1 Query: 2767 DSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEI--------PAWKPTVLKTLISRRDIN-QSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQ 3564 ++C +PCK+ G C + Y C C F GKNC++ +N C PC+NGG+CKN +G Y C C GF G C +V++C N C NG C + + YSC C++GF GK C+ C+ NPCQN G C+ + Y C C G+ GK CE++ ++C N CK+ C + H Y C C+ G+ GK CE P V K ++ T +N E +V + C+ + C N G+C +N++ C CK G++G +C+ Sbjct: 990 ETCSSKPCKNGGTCREVNGA-YSCTCKSGFTGKNCEQDVNECSKNPCKNGGVCKN--EHGGYSCACKAGFTGKNCEQDVNECNKNPCQNGGVCKNEHGGYSCACKAGFTGKNCEQDMNECSK-NPCQNGGVCK-NEHGGYSCACKAGFTGKNCEQDVNECSKNPCKNGGVCKNEHGGYSCACKAGFTGKNCEQDMNECSKNPCQNGGVCKNKHGGYSCACKAGFTGKNCEQDV---NECSKNPCKNGGVC----KNEHGGYSCTCKAGFTGKICE 1252 HSP 3 Score: 154.066 bits (388), Expect = 1.997e-37 Identity = 98/275 (35.64%), Postives = 141/275 (51.27%), Query Frame = 1 Query: 2767 DSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEI--------PAWKPTVLKTLISRRDIN-QSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQ 3564 + C K PC++ G C H Y C C F GKNC++ +N C PC+NGG+CKN +G Y C C GF G C +V++C N C NG C + + YSC C++GF GK C+ C+ NPCQN G C+ + Y C C G+ GK CE++ ++C N CK+ C + H Y C C+ G+ GK CE P V K ++ T +N E +V + C+ + C N G+C +N++ C CK G++G C+ Sbjct: 1066 NECNKNPCQNGGVCKN-EHGGYSCACKAGFTGKNCEQDMNECSKNPCQNGGVCKN--EHGGYSCACKAGFTGKNCEQDVNECSKNPCKNGGVCKNEHGGYSCACKAGFTGKNCEQDMNECSK-NPCQNGGVCKNK-HGGYSCACKAGFTGKNCEQDVNECSKNPCKNGGVCKNEHGGYSCTCKAGFTGKICEQDVNECSKNPCKNGGVCKNEHGGYSCTCKAGFTGKNCEQDV---NECSKNPCKNGGVC----KNEHGGYSCTCKAGFTGKNCE 1328 HSP 4 Score: 151.369 bits (381), Expect = 1.288e-36 Identity = 98/275 (35.64%), Postives = 139/275 (50.55%), Query Frame = 1 Query: 2767 DSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEI--------PAWKPTVLKTLISRRDIN-QSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQ 3564 + C K PCK+ G C H Y C C F GKNC++ +N C PC+NGG+CKN +G Y C C GF G C +V++C N C NG C + + YSC C+ GF GK C+ C +NPC+N G C+ + Y C C G+ GK CE++ ++C N CK+ C + H Y C C+ G+ GK CE P V K ++ T +N E +V + C+ + C N G+C +N++ C CK G+ G C+ Sbjct: 218 NECSKNPCKNGGVCKN-EHGGYSCACKVGFTGKNCEQDVNECSVNPCKNGGVCKN--EHGGYSCACKAGFTGKNCEQDVNECSVNPCKNGGVCKNEHGGYSCACKVGFTGKNCEQDVNEC-SVNPCKNGGVCK-NEHGGYSCACKAGFTGKNCEQDVNECSKNPCKNGGVCKNEHGGYSCACKAGFTGKNCEQDVNECSKNPCKNGGVCKNEHGGYSCACKAGFTGKNCEQDV---NECSKNPCQNGGVC----KNEHGGYSCACKAGFMGKNCE 480 HSP 5 Score: 149.828 bits (377), Expect = 3.622e-36 Identity = 85/222 (38.29%), Postives = 118/222 (53.15%), Query Frame = 1 Query: 2767 DSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCE----------IPAW----KPTVLKTLISRR 3390 + C K PC++ G C H Y C C F GK C++ +N C PC+NGG+CKN +G Y C C GF G C +V++C N C NG C + + YSC C++GF GK C+ CN NPCQN G C+ + Y C C G+ GK CE++ ++C N CK+ C + H Y C C+ G+ GK CE P W PTV + +++R+ Sbjct: 728 NECSKNPCQNGGVCKN-EHGGYSCACKAGFTGKICEQDVNECNKNPCQNGGVCKN--EHGGYSCTCKAGFTGKNCEQDVNECNKNPCQNGGVCKNEHGGYSCTCKAGFTGKNCEQDVNECNK-NPCQNGGVCK-NEHGGYSCTCKAGFTGKNCEQDVNECSKNPCKNGGVCKNEHGGYSCACKAGFTGKICEQEICEICGTHAPGWMEGSHPTVAEGIVTRK 944 HSP 6 Score: 147.902 bits (372), Expect = 1.358e-35 Identity = 96/271 (35.42%), Postives = 139/271 (51.29%), Query Frame = 1 Query: 2767 DSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVIS----ESVCAYHECLNNGICINGDQNKNQKSY-CKCKPGYSGHLCQ 3564 + C K PCK+ G C H Y C C F GK C++ +N C PC+NGG+CKN +G Y C C GF G C +V++C N C NG C + + YSC C++GF GK C+ C+ NPC+N G C+ + Y C C G+ GK CE++ ++C N CK+ C + H Y C C+ G+ GK +L + + + +E +E+ V++ ++ C + CLNNG CIN SY C C Y+G C+ Sbjct: 1218 NECSKNPCKNGGVCKN-EHGGYSCTCKAGFTGKICEQDVNECSKNPCKNGGVCKN--EHGGYSCTCKAGFTGKNCEQDVNECSKNPCKNGGVCKNEHGGYSCTCKAGFTGKNCEQDVNECSK-NPCKNGGVCKN-EHGGYSCTCKAGFTGKNCEQDVNECSKNPCKNGGVCKNEHGGYSCTCKAGFTGKT------ANKMLTNVPASTRVIMAESALTAMEDTVVNARPDKNECKVNPCLNNGKCIN-----TPGSYKCNCIDEYTGKHCE 1472 HSP 7 Score: 134.806 bits (338), Expect = 1.638e-31 Identity = 112/412 (27.18%), Postives = 165/412 (40.05%), Query Frame = 1 Query: 2281 YLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVLSFNKLQCIHQDAFKNLHN-----LKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKCD----SCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSG------------------------------------QCQLLPN--NDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKT 3375 + +N Q +NE S N + G + K T K+ E V + C + KN H KV N + + N KN + C C F + + ++ C S K + ++ + C+ + + C+ C K PCK+ G C H Y C C F GKNC++ +N C PC+NGG+CKN +G Y C C GF G C +V++C N C NG C + + YSC C++GF GK C+ C+ NPC+N G +C + N Y C C G+ GK CE++ ++C N CK+ C + H Y C C+ G+ GK CE KP ++ Sbjct: 246 FTGKNCEQDVNECSVNPCKNGGVCKNEHGGYSCACKAGFT-GKNCEQDVNECSVNPCKNGGVCKNEHGGYSCACKVGFTGKNCEQDVNECSVNPCKNGGVCKNEHGGYSCACKA-----GFTGKNCEQDVNEC-SKNPCKNGGVCKNEHGGYSCA--CKAGFTGKNCEQDVNECSKNPCKNGGVCKN-EHGGYSCACKAGFTGKNCEQDVNECSKNPCQNGGVCKN--EHGGYSCACKAGFMGKNCEQDVNECSKNPCKNGGVCKNEHGGYSCACKAGFTGKNCEQDVNECSK-NPCKNGGVCKNEHGGYSCACKAGFTGKNCEQDGLKTIIHLQSIKCGVCKNEHGGYSCACKAGFTGKNCEQDVNECSVNPCKNGGVCKNEHGGYSCACKAGFTGKNCEQAPSKPRECRS 644 HSP 8 Score: 129.413 bits (324), Expect = 5.770e-30 Identity = 83/237 (35.02%), Postives = 122/237 (51.48%), Query Frame = 1 Query: 2881 INACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEI--------PAWKPTVLKTLISRRDIN-QSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQ 3564 +N C PC+NGG+CKN +G Y C C VGF G C +V++C N C NG C + + YSC C++GF GK C+ C +NPC+N G C+ + Y C C G+ GK CE++ ++C N CK+ C + H Y C C+ G+ GK CE P V K ++ T +N E +V + C+ + C N G+C +N++ C CK G++G C+ Sbjct: 217 VNECSKNPCKNGGVCKN--EHGGYSCACKVGFTGKNCEQDVNECSVNPCKNGGVCKNEHGGYSCACKAGFTGKNCEQDVNEC-SVNPCKNGGVCK-NEHGGYSCACKVGFTGKNCEQDVNECSVNPCKNGGVCKNEHGGYSCACKAGFTGKNCEQDVNECSKNPCKNGGVCKNEHGGYSCACKAGFTGKNCEQDV---NECSKNPCKNGGVC----KNEHGGYSCACKAGFTGKNCE 442 HSP 9 Score: 122.479 bits (306), Expect = 8.407e-28 Identity = 78/227 (34.36%), Postives = 108/227 (47.58%), Query Frame = 1 Query: 2767 DSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCER------------------------------NTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWK 3357 + C K PCK+ G C H Y C C F GKNC++ +N C PC+NGG+CKN +G Y C C GF G C +V++C N C NG C + + YSC C++GF GK C+ C+ NPC+N G C+ + Y C C G+ GK + + ++CK N C + +C++ YKC C Y GK CE +K Sbjct: 1256 NECSKNPCKNGGVCKN-EHGGYSCTCKAGFTGKNCEQDVNECSKNPCKNGGVCKN--EHGGYSCTCKAGFTGKNCEQDVNECSKNPCKNGGVCKNEHGGYSCTCKAGFTGKNCEQDVNECSK-NPCKNGGVCK-NEHGGYSCTCKAGFTGKTANKMLTNVPASTRVIMAESALTAMEDTVVNARPDKNECKVNPCLNNGKCINTPGSYKCNCIDEYTGKHCETAKYK 1477
BLAST of slit1 vs. Ensembl Nematostella
Match: EDO46513 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RPI0]) HSP 1 Score: 148.288 bits (373), Expect = 1.000e-39 Identity = 91/252 (36.11%), Postives = 132/252 (52.38%), Query Frame = 1 Query: 2767 DSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEI--------PAWKPTVLKTLISRRDIN-QSEMTYENLENEVISESVCAYHECLNNGICIN 3495 ++C +PCK+ G C + Y C C F GKNC++ +N C PC+NGG+CKN +G Y C C GF G C +V++C N C NG C + + YSC C++GF GK C+ C++ NPC+N G C+ + Y C C G+ GK CE++ ++C N C++ C + H Y C C+ G+ GK CE P V K ++ T +N E + ++ C + CLNNG CIN Sbjct: 2 ETCSSKPCKNGGTCREVNGA-YSCTCKSGFTGKNCEQDVNECSKNPCKNGGVCKN--EHGGYSCACKAGFTGKNCEQDVNECSENPCKNGGVCKNEHGGYSCACKAGFTGKNCEQDVNECSE-NPCKNGGVCK-NEHGGYSCACKAGFTGKNCEQDVNECSKNPCQNGGVCKNEHGGYSCACKAGFTGKICEQDVNECNKNPCQNGGVCKNEHGGYSCTCKAGFTGKNCEQD---KNECKVNPCLNNGKCIN 245 HSP 2 Score: 132.494 bits (332), Expect = 2.925e-34 Identity = 75/182 (41.21%), Postives = 103/182 (56.59%), Query Frame = 1 Query: 2767 DSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMC 3312 + C + PCK+ G C H Y C C F GKNC++ +N C PC+NGG+CKN +G Y C C GF G C +V++C N C NG C + + YSC C++GF GK C+ CN NPCQN G C+ + Y C C G+ GK CE++ ++CK N C + +C++ YKC C Sbjct: 78 NECSENPCKNGGVCKN-EHGGYSCACKAGFTGKNCEQDVNECSENPCKNGGVCKN--EHGGYSCACKAGFTGKNCEQDVNECSKNPCQNGGVCKNEHGGYSCACKAGFTGKICEQDVNECNK-NPCQNGGVCK-NEHGGYSCTCKAGFTGKNCEQDKNECKVNPCLNNGKCINTPGSYKCNC 254 HSP 3 Score: 117.087 bits (292), Expect = 5.336e-29 Identity = 74/228 (32.46%), Postives = 115/228 (50.44%), Query Frame = 1 Query: 2881 INACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQ 3564 + C +PC+NGG C+ ++ G Y C C GF G C +V++C N C NG C + + YSC C++GF GK C+ C++ NPC+N G C+ + Y C C G+ GK CE++ ++C N CK+ C + H Y C C+ G+ GK CE +D+N+ C+ + C N G+C +N++ C CK G++G +C+ Sbjct: 1 LETCSSKPCKNGGTCREVN--GAYSCTCKSGFTGKNCEQDVNECSKNPCKNGGVCKNEHGGYSCACKAGFTGKNCEQDVNECSE-NPCKNGGVCK-NEHGGYSCACKAGFTGKNCEQDVNECSENPCKNGGVCKNEHGGYSCACKAGFTGKNCE---------------QDVNE-----------------CSKNPCQNGGVC----KNEHGGYSCACKAGFTGKICE 188
BLAST of slit1 vs. Ensembl Nematostella
Match: EDO39165 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SB01]) HSP 1 Score: 152.91 bits (385), Expect = 3.521e-37 Identity = 92/266 (34.59%), Postives = 132/266 (49.62%), Query Frame = 1 Query: 2767 DSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQ 3564 D C PCKH G C + C C + + G C+ +N C PC NGG+C D G Y+C+CP GF G C +N+D+C + C NGATC DL + + C+CQ GF+G+ C+ C C N C L N Y+C C G+ G+ C+ + D+C+ N C+H S C+D + Y+C+C+PGY G++C I DI++ C CLNNG+C+ + +C C G+SG C Sbjct: 130 DECQSSPCKHQGTCQNYLG-GFNCLCRHGYSGITCEVDVNECSSSPCVNGGVCA--DGLGEYKCICPSGFSGENCQVNIDECASSPCKNGATCEDLVDEFRCQCQPGFKGQNCETNINECIGA-ACANGSTCIDLVNA-YQCTCLPGFTGRHCDVDIDECQSNPCQHGSACMDGVSSYQCICQPGYTGQYCHI---------------DIDE-----------------CLSRPCLNNGMCL----DLVSDFHCTCPTGFSGKDCS 354 HSP 2 Score: 144.821 bits (364), Expect = 1.125e-34 Identity = 116/358 (32.40%), Postives = 157/358 (43.85%), Query Frame = 1 Query: 2767 DSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIP---------AWKPTVLKTLIS--------------RRDINQSEM------TYENLENE--------------VISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQ-----------YLNSIYFQ-KNSYIEISPPSRGFLMPRGNISLNFMTNS 3675 D C PCK+ C + ++C C F G+NC+ IN C G C NG C ID Y+C C GF G C +++D+C N C +G+ C D +SY C CQ G+ G+YC I C PC N+G C L +D+ C C G+ GK C N DDC C+H S C+DL NDYKC C GY GK C I + T L L +DI+ T E+L N + SVC + C N G C + N + C+C GY+GH C+ Y N+ N+Y I P RGF +++++ +S Sbjct: 206 DECASSPCKNGATCEDLVDE-FRCQCQPGFKGQNCETNINECIGAACANGSTC--IDLVNAYQCTCLPGFTGRHCDVDIDECQSNPCQHGSACMDGVSSYQCICQPGYTGQYCHIDIDECLS-RPCLNNGMCLDLV-SDFHCTCPTGFSGKDCSVNIDDCADAPCQHGSTCIDLVNDYKCSCLQGYTGKDCHIDINECSSNPCQYGGTCLNLLNGYACLCIDGYTGLNCEKDIDDCLFNPCHNGTCEDLVNNYKCVCPDNRQERTCAGNSSVCDTYLCRNGGSCFS---NNSTYYTCECPKGYTGHDCESKINYCKSSSCYGNATCLDGANNYTCICP--RGFTGTLCDVNVDECEDS 553 HSP 3 Score: 139.428 bits (350), Expect = 5.722e-33 Identity = 93/272 (34.19%), Postives = 131/272 (48.16%), Query Frame = 1 Query: 2767 DSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPA--WKPTVLKTLISRRDINQSE----MTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQ 3564 D C PC + G C T++ ++C C + G C+ I+ C +PC + G C + NY + C C VG+ G C +N+DDC + C +G +C DL N Y C C +GF+G C+ C NPC N+G C + Y CVC G+ G +CE N DDC + C + + C DL ND+ C C GY G+ C I P K + D M N I C + CLNNG C++G + C C G++G C+ Sbjct: 624 DECSSSPCVNGGLCVDYTNY-FECLCHPGYGGDRCEINIDDCANKPCLHNGNCTDYVNY--FTCACAVGYTGRTCEVNIDDCSPSPCRHGGSCQDLVNGYLCHCPAGFKGSKCETDIDECA-TNPCDNNGTC-VDRVASYDCVCKPGFTGTRCEANIDDCATSPCLNNAICTDLINDFHCACPAGYQGQTCAINVDDCTPQPCKQGGTCVDAVSGYTCLCMPGFTGINCSIEMDECGSYPCLNNGTCVDG----RNRVTCTCAVGFTGQFCE 886 HSP 4 Score: 137.117 bits (344), Expect = 2.942e-32 Identity = 98/305 (32.13%), Postives = 147/305 (48.20%), Query Frame = 1 Query: 2692 SIYHSKPTDFICSEETEDAYITAKCDS----CMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCE--IPAWKPTVLKTLISRRDINQSEMTY-------ENLENEVISESVCAYHECLNNGICINGDQNKNQKSY-CKCKPGYSGHLCQ 3564 S + + T + C E Y C+S C C N C L +NY C CP F G CD ++ C PC NG C+++ N ++ C CP GF+G C +++++C + C+N A C D+ + Y C C+SG+ G C+ C+ +PC N G C + N ++C+C G+ G +CE N DDC C H C D N + C C GY G+ CE I P+ + S +D+ + + E ++ CA + C NNG C++ SY C CKPG++G C+ Sbjct: 483 SCFSNNSTYYTC--ECPKGYTGHDCESKINYCKSSSCYGNATC-LDGANNYTCICPRGFTGTLCDVNVDECEDSPCANGASCEDLIN--DFRCHCPAGFQGRTCAVDINECATSPCLNEAKCTDVVSGYRCTCRSGYTGTRCERDIDECSS-SPCVNGGLC-VDYTNYFECLCHPGYGGDRCEINIDDCANKPCLHNGNCTDYVNYFTCACAVGYTGRTCEVNIDDCSPSPCRHGGSCQDLVNGYLCHCPAGFKGSKCETDI---DECATNPCDNNGTCVD-----RVASYDCVCKPGFTGTRCE 772 HSP 5 Score: 136.732 bits (343), Expect = 3.859e-32 Identity = 90/272 (33.09%), Postives = 126/272 (46.32%), Query Frame = 1 Query: 2767 DSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCE--IPAWKPTVLKTLISRRDI--NQSEMTYENLENEVISESV--CAYHECLNNGICINGDQNKNQKSY-CKCKPGYSGHLC 3561 D C PC+H G C + + Y C CP F G C+ I+ C PC N G C +D +Y+CVC GF G +C N+DDC + C+N A C DL N + C C +G++G+ C I C PC+ G C + + Y C+C G+ G C D+C C + CVD N C C G+ G+FCE I P + + +D+ + + Y E + + C H C NN C + K Y C C G +G C Sbjct: 700 DDCSPSPCRHGGSCQDLVN-GYLCHCPAGFKGSKCETDIDECATNPCDNNGTC--VDRVASYDCVCKPGFTGTRCEANIDDCATSPCLNNAICTDLINDFHCACPAGYQGQTCAINVDDCTP-QPCKQGGTC-VDAVSGYTCLCMPGFTGINCSIEMDECGSYPCLNNGTCVDGRNRVTCTCAVGFTGQFCEVNIDDCDPAPCQNYGTCQDLVADFTCHCYPGFTGETCATPIDLCEAHPCQNNATC----RMDPLKGYSCSCPTGVTGRHC 962 HSP 6 Score: 135.961 bits (341), Expect = 6.474e-32 Identity = 73/208 (35.10%), Postives = 109/208 (52.40%), Query Frame = 1 Query: 2722 ICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEI 3345 +C + ++ C C++ G C Y C+CP + G +C+ +IN C C C +D NY C+CP GF G C +NVD+C + C NGA+C DL N + C C +GF+G+ C + C +PC N +C + + Y+C C G+ G +CER+ D+C + C + CVD N ++C+C PGY G CEI Sbjct: 456 VCPDNRQERTCAGNSSVCDTYLCRNGGSCFSNNSTYYTCECPKGYTGHDCESKINYCKSSSCYGNATC--LDGANNYTCICPRGFTGTLCDVNVDECEDSPCANGASCEDLINDFRCHCPAGFQGRTCAVDINECA-TSPCLNEAKCTDVVSG-YRCTCRSGYTGTRCERDIDECSSSPCVNGGLCVDYTNYFECLCHPGYGGDRCEI 659 HSP 7 Score: 128.642 bits (322), Expect = 9.635e-30 Identity = 91/272 (33.46%), Postives = 120/272 (44.12%), Query Frame = 1 Query: 2767 DSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIP---AWKPTVLKTLISRRDI--NQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSY-CKCKPGYSGHLCQ 3564 D C PC +NG C + +Y C C F G C+ I+ C PC N IC ++ N ++ C CP G++G C INVDDC C G TC D + Y+C C GF G C I+ C PC N+G C + N C C+ G+ G+ CE N DDC C++ C DL D+ C C PG+ G+ C P R D S + S+C C NNG C+ Q Y C C ++ CQ Sbjct: 738 DECATNPCDNNGTC-VDRVASYDCVCKPGFTGTRCEANIDDCATSPCLNNAICTDLIN--DFHCACPAGYQGQTCAINVDDCTPQPCKQGGTCVDAVSGYTCLCMPGFTGINCSIEMDECGSY-PCLNNGTC-VDGRNRVTCTCAVGFTGQFCEVNIDDCDPAPCQNYGTCQDLVADFTCHCYPGFTGETCATPIDLCEAHPCQNNATCRMDPLKGYSCSCPTGVTGRHCDVSICDSKPCRNNGSCV-----LTQSGYQCLCASSFTSPQCQ 999 HSP 8 Score: 127.487 bits (319), Expect = 2.346e-29 Identity = 89/299 (29.77%), Postives = 137/299 (45.82%), Query Frame = 1 Query: 2716 DFICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQC-VDLHNDYKCMCRPGYIGKFCEIPAW--KP-----TVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNSIYFQ 3588 D +C A D C PC +N C+ + + ++ C CP + G+ C ++ C QPC+ GG C +D Y C+C GF G C+I +D+C C+N TC D N +C C GF G++C++ C D PCQN G CQ L D+ C C G+ G+ C D C+ + C++ + C +D Y C C G G+ C++ KP + + T + + S T + + C + CLN C++ D C C GY G C+ ++ + Q Sbjct: 759 DCVCKPGFTGTRCEANIDDCATSPCLNNAICTDLIN-DFHCACPAGYQGQTCAINVDDCTPQPCKQGGTC--VDAVSGYTCLCMPGFTGINCSIEMDECGSYPCLNNGTCVDGRNRVTCTCAVGFTGQFCEVNIDDC-DPAPCQNYGTCQDLVA-DFTCHCYPGFTGETCATPIDLCEAHPCQNNATCRMDPLKGYSCSCPTGVTGRHCDVSICDSKPCRNNGSCVLTQSGYQCLCASSFTSPQCQRSIDD---CEHAHCLNGATCVDSD----NAYKCSCLEGYYGKHCELIDRCHSQ 1045 HSP 9 Score: 122.865 bits (307), Expect = 5.923e-28 Identity = 89/287 (31.01%), Postives = 132/287 (45.99%), Query Frame = 1 Query: 2767 DSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCN-DLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNSIYFQKNSYIEISPPSR 3624 D C PC +NG C + + C C F G+ C+ I+ C PC+N G C+++ ++ C C GF G C +D C + C N ATC D YSC C +G G++CD+ D PC+N+G C +L + Y+C+C+ + QC+R+ DDC++ C + + CVD N YKC C GY GK CE LI R C C N C++ D +CKC G++G C+ + + + ++ PSR Sbjct: 852 DECGSYPCLNNGTC-VDGRNRVTCTCAVGFTGQFCEVNIDDCDPAPCQNYGTCQDL--VADFTCHCYPGFTGETCATPIDLCEAHPCQNNATCRMDPLKGYSCSCPTGVTGRHCDVSIC---DSKPCRNNGSC-VLTQSGYQCLCASSFTSPQCQRSIDDCEHAHCLNGATCVDSDNAYKCSCLEGYYGKHCE-----------LIDR----------------------CHSQPCQNGATCVDDD----GVVFCKCAMGFTGTHCEKEFNHCIVRVADTNLTFPSR 1094 HSP 10 Score: 114.005 bits (284), Expect = 2.726e-25 Identity = 90/323 (27.86%), Postives = 138/323 (42.72%), Query Frame = 1 Query: 2773 CMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPN-------------------------------------NDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCE--------------------IPAWKPTVLKTLISRR-DINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSY-CKCKPGYSGHLCQ 3564 C C + G CS ++ + C C F G++C+ ++ C C NG C ID +++C+CPVGF G C N +DC N C N ATC DL N +SC+C G G+ C+ C +PC++ G CQ +YKC+C G+ G+ C+ N D+C + CK+ + C DL ++++C C+PG+ G+ CE + A++ T L R D++ E C + C + C++G SY C C+PGY+G C Sbjct: 18 CQHDSCLNGGTCSEGSN-SLLCSCLPGFTGQSCETDVDECKLVQCHNGATC--IDQVNSFKCICPVGFHGILCETNYNDCHSNPCQNNATCQDLINDFSCQCPQGITGQTCNTDIDECQS-SPCKHQGTCQNYLGGFNCLCRHGYSGITCEVDVNECSSSPCVNGGVCADGLGEYKCICPSGFSGENCQVNIDECASSPCKNGATCEDLVDEFRCQCQPGFKGQNCETNINECIGAACANGSTCIDLVNAYQCTCLPGFTGRHCDVDIDE---------------CQSNPCQHGSACMDG-----VSSYQCICQPGYTGQYCH 316 HSP 11 Score: 112.464 bits (280), Expect = 9.708e-25 Identity = 104/361 (28.81%), Postives = 144/361 (39.89%), Query Frame = 1 Query: 2626 FLDRFLDNGISLCAS-PEKMKLKSIYHSKPTDFICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCV---------------------------------------CSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEI--------PAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQ 3564 F R D I C S P + + IC Y D C+ RPC +NG C L ++ C CP F GK+C I+ C PC++G C ID +Y+C C G+ G C I++++C N C G TC +L N Y+C C G+ G C+ C NPC N G C+ L NN YKCV C KG+ G CE + CK +SC + C+D N+Y C+C G+ G C++ P + LI+ D + + CA CLN C + C C+ GY+G C+ Sbjct: 272 FTGRHCDVDIDECQSNPCQHGSACMDGVSSYQCICQPGYTGQYCHIDIDECLSRPCLNNGMC-LDLVSDFHCTCPTGFSGKDCSVNIDDCADAPCQHGSTC--IDLVNDYKCSCLQGYTGKDCHIDINECSSNPCQYGGTCLNLLNGYACLCIDGYTGLNCEKDIDDCL-FNPCHN-GTCEDLVNN-YKCVCPDNRQERTCAGNSSVCDTYLCRNGGSCFSNNSTYYTCECPKGYTGHDCESKINYCKSSSCYGNATCLDGANNYTCICPRGFTGTLCDVNVDECEDSPCANGASCEDLIN--DFRCHCPAGFQGRTCAVDINECATSPCLNEAKCT----DVVSGYRCTCRSGYTGTRCE 620 HSP 12 Score: 105.916 bits (263), Expect = 9.537e-23 Identity = 89/332 (26.81%), Postives = 135/332 (40.66%), Query Frame = 1 Query: 2401 PLKSLETLVLSFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFIC------SEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDC-----------KYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEI 3345 P K T V + + C+ F ++ ++ ++ + P N G+N C C + + QF D P + DF C + ET I D C PC++N C + Y C CP G++CD I C +PCRN G C + Y+C+C F P+C ++DDC C+NGATC D +N+Y C C G+ GK+C++ C+ PCQN C + + C C+ G+ G CE+ + C C CV + C+C+PG+ G C+ Sbjct: 820 PCKQGGTCVDAVSGYTCLCMPGFTGIN----CSIEMDECGSYP------CLNNGTCVDGRNRVTCTCAVGFTGQFCEVNIDD------CDPAPCQNYGTCQDLVADFTCHCYPGFTGETCATPI----DLCEAHPCQNNATCRMDPLKGYSCSCPTGVTGRHCDVSI--CDSKPCRNNGSC--VLTQSGYQCLCASSFTSPQCQRSIDDCEHAHCLNGATCVDSDNAYKCSCLEGYYGKHCEL-IDRCHS-QPCQNGATC-VDDDGVVFCKCAMGFTGTHCEKEFNHCIVRVADTNLTFPSRICGDHGTCVSKPTGFLCVCQPGFTGLLCQT 1124 HSP 13 Score: 87.4261 bits (215), Expect = 4.072e-17 Identity = 65/190 (34.21%), Postives = 90/190 (47.37%), Query Frame = 1 Query: 2773 CMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYC----NDIN------PCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKH-ESQCVDLHNDYKCM 3309 C +PC++NG C ++T YQC C F C + I+ C C NG C + DN Y+C C G+ G C + +D C C NGATC D + CKC GF G +C+ +F +C D N C + G C P + CVC G+ G C+ + DDC + C CVD + + C Sbjct: 967 CDSKPCRNNGSC-VLTQSGYQCLCASSFTSPQCQRSIDDCEHAHCLNGATCVDSDNA--YKCSCLEGYYGKHCEL-IDRCHSQPCQNGATCVDDDGVVFCKCAMGFTGTHCEKEFNHCIVRVADTNLTFPSRICGDHGTCVSKPTG-FLCVCQPGFTGLLCQTDIDDCSSDPCVGINVTCVDGIDTFTCQ 1151
BLAST of slit1 vs. Ensembl Nematostella
Match: EDO42644 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S0V1]) HSP 1 Score: 139.428 bits (350), Expect = 5.396e-37 Identity = 83/193 (43.01%), Postives = 101/193 (52.33%), Query Frame = 1 Query: 2767 DSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEI 3345 D C PCK+ G C + + Y C C F GKNC+ I+ C G PC NGG CK D + C C VG+ G C I++D+C C NGATCND N+Y+C C GF G C+ C PCQN G C NN Y C+C G+ G CE N D+C N C + C D N + C C GY G CEI Sbjct: 34 DECKSSPCKNGGTC-VDGINRYSCTCVAGFTGKNCETDIDECAGNPCANGGTCK--DGINGFTCECAVGYNGSTCEIDIDECASGPCQNGATCNDGVNNYTCSCIPGFNGTNCETNIDECAS-GPCQNGGTCNDGVNN-YTCLCIPGFNGTNCETNIDECIGNPCANGGTCKDGINGFTCECPVGYSGSTCEI 221 HSP 2 Score: 122.094 bits (305), Expect = 4.692e-31 Identity = 82/244 (33.61%), Postives = 109/244 (44.67%), Query Frame = 1 Query: 2833 QCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQ 3564 +C CP F G C+ + C PC+NGG C +D Y C C GF G C ++D+C GN C NG TC D N ++C+C G+ G C+I C PCQN C NN Y C C G+ G CE N D+C C++ C D N+Y C+C PG+ G CE +I++ C + C N G C +G C+C GYSG C+ Sbjct: 17 RCHCPAGFSGDKCENHDDECKSSPCKNGGTC--VDGINRYSCTCVAGFTGKNCETDIDECAGNPCANGGTCKDGINGFTCECAVGYNGSTCEIDIDECAS-GPCQNGATCNDGVNN-YTCSCIPGFNGTNCETNIDECASGPCQNGGTCNDGVNNYTCLCIPGFNGTNCET---------------NIDE-----------------CIGNPCANGGTCKDGIN----GFTCECPVGYSGSTCE 220
BLAST of slit1 vs. Ensembl Nematostella
Match: EDO36195 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SJC2]) HSP 1 Score: 140.969 bits (354), Expect = 1.304e-36 Identity = 97/269 (36.06%), Postives = 124/269 (46.10%), Query Frame = 1 Query: 2773 CMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDI-----NPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQ 3564 C PC++ G C + Y C C ++G NC+ IN C PC NGG C D YECVC G+ G +C I++D+C + CVNG TC D N+Y+C C GF G + + C DI NPC N G C + N Y C C G+ G CE + ++C N C + C DL N Y C C PGY G CEI DIN+ CA + C+N G C + N C C PGY G C+ Sbjct: 79 CTSAPCQNGGSC-VDRRDGYTCTCEAGYNGINCEIEINECDSGPCNNGGTCT--DRVNGYECVCNAGYTGTRCEIDIDECTSSPCVNGGTCTDAVNNYTCACLPGFTGYF----IVTCADIDECASNPCINGGTCTDMVNG-YNCTCPPGYNGTTCEIDINECASNPCLNGGTCNDLVNGYNCNCPPGYNGTTCEI---------------DINE-----------------CASNPCVNGGTCADLVNGFN----CTCPPGYKGTKCE 303 HSP 2 Score: 129.028 bits (323), Expect = 1.678e-32 Identity = 81/265 (30.57%), Postives = 124/265 (46.79%), Query Frame = 1 Query: 2773 CMKRPCKHNGKCSL-ITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQ 3564 C C H G C + +C C + G++C+ ++ C PC N G C +D ++ C CP+GF G +C + +C C NG +C D + Y+C C++G+ G C+I+ C D PC N G C N Y+CVC+ G+ G +CE + D+C + C + C D N+Y C C PG+ G F +++ DI++ CA + C+N G C + N C C PGY+G C+ Sbjct: 1 CASNKCVHGGTCEFDCSTLTSRCRCVAGYTGEHCEVNVDDCASSPCTNNGTC--VDGINSFACTCPLGFTGTRCETEIKECTSAPCQNGGSCVDRRDGYTCTCEAGYNGINCEIEINEC-DSGPCNNGGTCTDRVNG-YECVCNAGYTGTRCEIDIDECTSSPCVNGGTCTDAVNNYTCACLPGFTGYF-------------IVTCADIDE-----------------CASNPCINGGTCTDMVNGYN----CTCPPGYNGTTCE 227 HSP 3 Score: 100.138 bits (248), Expect = 1.286e-22 Identity = 63/157 (40.13%), Postives = 82/157 (52.23%), Query Frame = 1 Query: 2767 DSCMKRPCKHNGKCSLITHHNYQCDCPYPFHG---KNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCE 3228 D C PC + G C+ + NY C C F G C I+ C PC NGG C ++ N Y C CP G+ G C I++++C N C+NG TCNDL N Y+C C G+ G C+I C NPC N G C L N + C C G++G +CE Sbjct: 153 DECTSSPCVNGGTCTDAVN-NYTCACLPGFTGYFIVTC-ADIDECASNPCINGGTCTDMVN--GYNCTCPPGYNGTTCEIDINECASNPCLNGGTCNDLVNGYNCNCPPGYNGTTCEIDINECAS-NPCVNGGTCADLVNG-FNCTCPPGYKGTKCE 303 HSP 4 Score: 92.0485 bits (227), Expect = 7.337e-20 Identity = 48/120 (40.00%), Postives = 67/120 (55.83%), Query Frame = 1 Query: 2767 DSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYC 3126 D C PC + G C+ + + Y C CP ++G C+ IN C PC NGG C ++ N Y C CP G+ G C I++++C N CVNG TC DL N ++C C G++G C+I C Sbjct: 193 DECASNPCINGGTCTDMVN-GYNCTCPPGYNGTTCEIDINECASNPCLNGGTCNDLVN--GYNCNCPPGYNGTTCEIDINECASNPCVNGGTCADLVNGFNCTCPPGYKGTKCEIGKCVC 309
BLAST of slit1 vs. Ensembl Medaka
Match: slit2 (slit guidance ligand 2 [Source:NCBI gene;Acc:101173607]) HSP 1 Score: 711.835 bits (1836), Expect = 0.000e+0 Identity = 474/1409 (33.64%), Postives = 714/1409 (50.67%), Query Frame = 1 Query: 43 MIFIFKDITFKFLLVLLSSLYCFSSTIWINDPSDESEICPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQLQ------------------------CINDNIFNKLGMLKYLLLEGNKIFWISEA-ISKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCASEDKANLENMCPSDKKV-CSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQIYNFQCN--KEFESKYETHDEECVPKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLPPIYSIHEAPNLMKLDLSFNQIETLEPDVFTRSSK------------------------LREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQFHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLC-DGNASVYHSVDSCHETNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVLSFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYG-NYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCN-DINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNSIYF-QKNSYIEISPPSRGFLMPRGNISLNFMTNSSSGLILYQGSEQTYLVVNLYQGHLRISYNLGDKVVGPEG-YSMGMVNDSRLHALHIEIVGENLTMYLNGRVHCTLKSKYLNSNRYMALDTGIFIGGAPHKILQSAIMKFHIET-----DSEFIGCMESLLINRKKIDFSKYL 4083 M+ + + L +S L TI ++ +D ++ CP C C +V+C + +VP + T RL+L N + I K+DF L +L+VLQL N+IT+IE GAF +L L +LRLNRN + P+ LF TKL +LDL +NQ+Q CI D F L L+ L L N I +S A + + L+ R+H N L C+CH+AWL+ +L+Q+ + G ++ C +P L ++ + EF C + + + S + C + C CS++ IV C GK L+ IP +LP + E+ L+ N I ++ G S YK L++I L+ N + ++ AF GL L S+ L N + I K F GL SL L L N I CL D + L+LL L +NKL ++ K TF ++ L+L NP CDC + W+ DY++D T A C PR L + ++ F+C+ +++ SK +C ++CP KC C V+CSN L +P+ I T L +++N L + P+L K++LS N+I +E F +S LR + L SNR+ C++N +F L + +L L +N ++ + +F +L L L N F+C+C L W+ +W R V GN P CQ+P LK+ PI + + F C DGN + +SC CS T V CS+ L +P +PK+TTELYLD NH + N +L + LS N I+ L+ + L+ + L TL+LS+N+L+CI AF L +L++L L ND+S IP AF DL +L+++ALG NP +CDC+++WL+ + + + GI+ CA P M K + + F C+ D I AKC+ C+ PCK++G C+ H Y+C CPY F G+NC+ I+AC PC+NGG C + G N+ CVCP GF G C IN+DDC N C N +TC D N+Y+C C + G+ C+ K +C ++NPCQ+ +C L P YKC C+ G+ G+ CE + DDCK N C++ +QC+D N Y C+C GY G FCE P VL S C HECLN C+ + C+C GY G C+ L S+ F + SY+++ PS L P+ NISL T+ SG++LY+G + ++ + LY+G LR+SY+ G P YS+ VND HA+ + + L++ ++G ++ S +N + +D+ +++GG P + S + + F GC+ +L IN K D L Sbjct: 1 MVGALRPAAYSRSLATVSGLLVI--TILLSSGAD-AQPCPTQCSCTGTTVDCHGQGLRSVPRNIPRNTERLDLNANNLTKITKADFAGLRHLRVLQLMENKITTIERGAFQDLKELERLRLNRNNLAVFPELLFLGTTKLHRLDLSENQIQGVPRKAFRGAVEIKNLQLDYNHISCIEDGAFRALRDLEVLTLNNNNISRLSVASFNHMPKLRTFRLHSNNLQCDCHVAWLSEWLRQRPRL---GLYTQ-CMSPPHLRGHNVAEVQKKEFVCTDAVEGHQSSSSSSCSVLQCPESCTCSNN-IVDCRGKGLTEIPTNLPETITEIRLEQNAIKVIPAGAFSPYKRLRRIDLSNNQISELASDAFQGLRSLNSLVLYGNKITEISKGLFEGLFSLQLLLLNANKIACLRVDAFQDLHNLNLLSLYDNKLQTIAKGTFSSLRAIQTLHLAQNPFICDCHLKWLADYLQDNPIETSGARCTSPRRLANKRIGQIKSKKFRCSGTEDYRSKL---GGDCFADLACPEKCRCEGTTVDCSNQKLTKIPDHIPQYTAELRLNNNEFTVLEATGIFKKLPHLRKINLSNNRITDIEEGTFEGASGVNELILTSNKLENIHHSMLKGLGGLRTLMLRSNRISCVSNSSFVGLSSVRLLSLYDNQVTSMTPGAFDTLHSLSTLNLLANPFNCNCHLAWLGDWLRRKRIVTGN---PRCQNPYFLKEIPIQDVAVQDFACEDGN-----NENSCSPVMRCPAECSC-----------------LDTVVRCSNKGLTTLPKGLPKETTELYLDGNHFTQV-PVELSNYKHLTLIDLSNNQISTLSNHSLSNMSELLTLILSYNRLRCIPVRAFDGLKSLRLLSLHGNDISLIPEGAFKDLSSLSHLALGANPLYCDCHMQWLSDWVKSGYKEPGIARCAGPGDMTDKLLLTTPSKKFTCTGPV-DLSIQAKCEPCLSNPCKNDGTCTNDPVHYYRCTCPYGFKGQNCEDPIHACISNPCQNGGTCHLKEGDGSNFWCVCPEGFEGDSCEINIDDCEDNDCENNSTCVDGINNYTCMCSPEYTGELCEEKLDFCAPELNPCQHDSKCILTPQG-YKCECTPGFVGEHCELDYDDCKDNKCQNGAQCIDAVNGYTCVCSEGYSGLFCEFAP--PMVLP-----------------------RTSPCDNHECLNGAQCVVVGTDPR----CQCLHGYEGERCETLVSVNFVNRESYLQL--PSN-LLSPQTNISLQIATDEDSGVLLYKG-DNDHIAMELYRGRLRVSYDTGS--YPPSAIYSVETVNDGSFHAVELVAAEQTLSLSIDGGPPKSIGS--INKQSTLNIDSPLYLGGMPERAFASGGFSALQHSPGGHNGTSFHGCLRNLYINGKLQDLGAGL 1333 HSP 2 Score: 56.9954 bits (136), Expect = 1.810e-7 Identity = 44/136 (32.35%), Postives = 66/136 (48.53%), Query Frame = 1 Query: 2899 QPCRN----GGICKNIDNYGNYECVCPVGFRGPKCTINVDD-CVGNLCVNGATCNDLNN-SYSCKCQSGFRGKYCDIKFLYCNDINPCQNS----GQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQ 3276 QPC+ G C G + C C G+ G C V + C GN C+ G TC +N+ SYSC+CQ GF G CD + + +N C S G+C++ C C+ G+ G+ C+R + + N + +Q Sbjct: 1359 QPCQRDTCVSGDCHPTGQRG-FTCTCHPGWTGTLCDQQVSNPCDGNKCIYG-TCVPINSYSYSCRCQPGFTGVLCDERDH--DTVNSCSLSHCKHGKCRVSGLGKAYCECNSGYTGEACDRASPELPLNPMRGATQ 1490
BLAST of slit1 vs. Ensembl Medaka
Match: slit2 (slit guidance ligand 2 [Source:NCBI gene;Acc:101173607]) HSP 1 Score: 704.901 bits (1818), Expect = 0.000e+0 Identity = 475/1425 (33.33%), Postives = 715/1425 (50.18%), Query Frame = 1 Query: 43 MIFIFKDITFKFLLVLLSSLYCFSSTIWINDPSDESEICPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQLQ------------------------CINDNIFNKLGMLKYLLLEGNKIFWISEA-ISKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCASEDKANLENMCPSDKKV-CSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQIYNFQCN----------KEFESKYETHDEECVPKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLPPIYSIHEAPNLMKLDLSFNQIETLEPDVFTRSSK------------------------LREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQFHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLC-DGNASVYHSVDSCHETNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVLSFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYG-NYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGF--------RGKYCDIKFLYCN-DINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNSIYF-QKNSYIEISPPSRGFLMPRGNISLNFMTNSSSGLILYQGSEQTYLVVNLYQGHLRISYNLGDKVVGPEG-YSMGMVNDSRLHALHIEIVGENLTMYLNGRVHCTLKSKYLNSNRYMALDTGIFIGGAPHKILQSAIMKFHIET-----DSEFIGCMESLLINRKKIDFSKYL 4083 M+ + + L +S L TI ++ +D ++ CP C C +V+C + +VP + T RL+L N + I K+DF L +L+VLQL N+IT+IE GAF +L L +LRLNRN + P+ LF TKL +LDL +NQ+Q CI D F L L+ L L N I +S A + + L+ R+H N L C+CH+AWL+ +L+Q+ + G ++ C +P L ++ + EF C + + + S + C + C CS++ IV C GK L+ IP +LP + E+ L+ N I ++ G S YK L++I L+ N + ++ AF GL L S+ L N + I K F GL SL L L N I CL D + L+LL L +NKL ++ K TF ++ L+L NP CDC + W+ DY++D T A C PR L + ++ F+C+ +++ SK +C ++CP KC C V+CSN L +P+ I T L +++N L + P+L K++LS N+I +E F +S LR + L SNR+ C++N +F L + +L L +N ++ + +F +L L L N F+C+C L W+ +W R V GN P CQ+P LK+ PI + + F C DGN + +SC CS T V CS+ L +P +PK+TTELYLD NH + N +L + LS N I+ L+ + L+ + L TL+LS+N+L+CI AF L +L++L L ND+S IP AF DL +L+++ALG NP +CDC+++WL+ + + + GI+ CA P M K + + F C+ D I AKC+ C+ PCK++G C+ H Y+C CPY F G+NC+ I+AC PC+NGG C + G N+ CVCP GF G C IN+DDC N C N +TC D N+Y+C C + RG+ C+ K +C ++NPCQ+ +C L P YKC C+ G+ G+ CE + DDCK N C++ +QC+D N Y C+C GY G FCE P VL S C HECLN C+ + C+C GY G C+ L S+ F + SY+++ PS L P+ NISL T+ SG++LY+G + ++ + LY+G LR+SY+ G P YS+ VND HA+ + + L++ ++G ++ S +N + +D+ +++GG P + S + + F GC+ +L IN K D L Sbjct: 1 MVGALRPAAYSRSLATVSGLLVI--TILLSSGAD-AQPCPTQCSCTGTTVDCHGQGLRSVPRNIPRNTERLDLNANNLTKITKADFAGLRHLRVLQLMENKITTIERGAFQDLKELERLRLNRNNLAVFPELLFLGTTKLHRLDLSENQIQGVPRKAFRGAVEIKNLQLDYNHISCIEDGAFRALRDLEVLTLNNNNISRLSVASFNHMPKLRTFRLHSNNLQCDCHVAWLSEWLRQRPRL---GLYTQ-CMSPPHLRGHNVAEVQKKEFVCTDAVEGHQSSSSSSCSVLQCPESCTCSNN-IVDCRGKGLTEIPTNLPETITEIRLEQNAIKVIPAGAFSPYKRLRRIDLSNNQISELASDAFQGLRSLNSLVLYGNKITEISKGLFEGLFSLQLLLLNANKIACLRVDAFQDLHNLNLLSLYDNKLQTIAKGTFSSLRAIQTLHLAQNPFICDCHLKWLADYLQDNPIETSGARCTSPRRLANKRIGQIKSKKFRCSAREQYFIPGTEDYRSKL---GGDCFADLACPEKCRCEGTTVDCSNQKLTKIPDHIPQYTAELRLNNNEFTVLEATGIFKKLPHLRKINLSNNRITDIEEGTFEGASGVNELILTSNKLENIHHSMLKGLGGLRTLMLRSNRISCVSNSSFVGLSSVRLLSLYDNQVTSMTPGAFDTLHSLSTLNLLANPFNCNCHLAWLGDWLRRKRIVTGN---PRCQNPYFLKEIPIQDVAVQDFACEDGN-----NENSCSPVMRCPAECSC-----------------LDTVVRCSNKGLTTLPKGLPKETTELYLDGNHFTQV-PVELSNYKHLTLIDLSNNQISTLSNHSLSNMSELLTLILSYNRLRCIPVRAFDGLKSLRLLSLHGNDISLIPEGAFKDLSSLSHLALGANPLYCDCHMQWLSDWVKSGYKEPGIARCAGPGDMTDKLLLTTPSKKFTCTGPV-DLSIQAKCEPCLSNPCKNDGTCTNDPVHYYRCTCPYGFKGQNCEDPIHACISNPCQNGGTCHLKEGDGSNFWCVCPEGFEGDSCEINIDDCEDNDCENNSTCVDGINNYTCMCSPEYTAATNEVERGELCEEKLDFCAPELNPCQHDSKCILTPQG-YKCECTPGFVGEHCELDYDDCKDNKCQNGAQCIDAVNGYTCVCSEGYSGLFCEFAP--PMVLP-----------------------RTSPCDNHECLNGAQCVVVGTDPR----CQCLHGYEGERCETLVSVNFVNRESYLQL--PSN-LLSPQTNISLQIATDEDSGVLLYKG-DNDHIAMELYRGRLRVSYDTGS--YPPSAIYSVETVNDGSFHAVELVAAEQTLSLSIDGGPPKSIGS--INKQSTLNIDSPLYLGGMPERAFASGGFSALQHSPGGHNGTSFHGCLRNLYINGKLQDLGAGL 1349 HSP 2 Score: 54.6842 bits (130), Expect = 1.078e-6 Identity = 42/121 (34.71%), Postives = 60/121 (49.59%), Query Frame = 1 Query: 2899 QPCRN----GGICKNIDNYGNYECVCPVGFRGPKCTINVDD-CVGNLCVNGATCNDLNN-SYSCKCQSGFRGKYCDIKFLYCNDINPCQNS----GQCQLLPNNDYKCVCSKGWEGKQCER 3231 QPC+ G C G + C C G+ G C V + C GN C+ G TC +N+ SYSC+CQ GF G CD + + +N C S G+C++ C C+ G+ G+ C+R Sbjct: 1375 QPCQRDTCVSGDCHPTGQRG-FTCTCHPGWTGTLCDQQVSNPCDGNKCIYG-TCVPINSYSYSCRCQPGFTGVLCDERDH--DTVNSCSLSHCKHGKCRVSGLGKAYCECNSGYTGEACDR 1491
BLAST of slit1 vs. Ensembl Medaka
Match: slit2 (slit guidance ligand 2 [Source:NCBI gene;Acc:101173607]) HSP 1 Score: 703.36 bits (1814), Expect = 0.000e+0 Identity = 474/1424 (33.29%), Postives = 713/1424 (50.07%), Query Frame = 1 Query: 43 MIFIFKDITFKFLLVLLSSLYCFSSTIWINDPSDESEICPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQLQ------------------------CINDNIFNKLGMLKYLLLEGNKIFWISEA-ISKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCASEDKANLENMCPSDKKVCSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQIYNFQCN----------KEFESKYETHDEECVPKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLPPIYSIHEAPNLMKLDLSFNQIETLEPDVFTRSSK------------------------LREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQFHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLC-DGNASVYHSVDSCHETNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVLSFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYG-NYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGF--------RGKYCDIKFLYCN-DINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNSIYF-QKNSYIEISPPSRGFLMPRGNISLNFMTNSSSGLILYQGSEQTYLVVNLYQGHLRISYNLGDKVVGPEG-YSMGMVNDSRLHALHIEIVGENLTMYLNGRVHCTLKSKYLNSNRYMALDTGIFIGGAPHKILQSAIMKFHIET-----DSEFIGCMESLLINRKKIDFSKYL 4083 M+ + + L +S L TI ++ +D ++ CP C C +V+C + +VP + T RL+L N + I K+DF L +L+VLQL N+IT+IE GAF +L L +LRLNRN + P+ LF TKL +LDL +NQ+Q CI D F L L+ L L N I +S A + + L+ R+H N L C+CH+AWL+ +L+Q+ + G ++ C +P L ++ + EF C ++ + C + C CS++ IV C GK L+ IP +LP + E+ L+ N I ++ G S YK L++I L+ N + ++ AF GL L S+ L N + I K F GL SL L L N I CL D + L+LL L +NKL ++ K TF ++ L+L NP CDC + W+ DY++D T A C PR L + ++ F+C+ +++ SK +C ++CP KC C V+CSN L +P+ I T L +++N L + P+L K++LS N+I +E F +S LR + L SNR+ C++N +F L + +L L +N ++ + +F +L L L N F+C+C L W+ +W R V GN P CQ+P LK+ PI + + F C DGN + +SC CS T V CS+ L +P +PK+TTELYLD NH + N +L + LS N I+ L+ + L+ + L TL+LS+N+L+CI AF L +L++L L ND+S IP AF DL +L+++ALG NP +CDC+++WL+ + + + GI+ CA P M K + + F C+ D I AKC+ C+ PCK++G C+ H Y+C CPY F G+NC+ I+AC PC+NGG C + G N+ CVCP GF G C IN+DDC N C N +TC D N+Y+C C + RG+ C+ K +C ++NPCQ+ +C L P YKC C+ G+ G+ CE + DDCK N C++ +QC+D N Y C+C GY G FCE P VL S C HECLN C+ + C+C GY G C+ L S+ F + SY+++ PS L P+ NISL T+ SG++LY+G + ++ + LY+G LR+SY+ G P YS+ VND HA+ + + L++ ++G ++ S +N + +D+ +++GG P + S + + F GC+ +L IN K D L Sbjct: 1 MVGALRPAAYSRSLATVSGLLVI--TILLSSGAD-AQPCPTQCSCTGTTVDCHGQGLRSVPRNIPRNTERLDLNANNLTKITKADFAGLRHLRVLQLMENKITTIERGAFQDLKELERLRLNRNNLAVFPELLFLGTTKLHRLDLSENQIQGVPRKAFRGAVEIKNLQLDYNHISCIEDGAFRALRDLEVLTLNNNNISRLSVASFNHMPKLRTFRLHSNNLQCDCHVAWLSEWLRQRPRL---GLYTQ-CMSPPHLRGHNVAEVQKKEFVCTGHQSSSSSSC---SVLQCPESCTCSNN-IVDCRGKGLTEIPTNLPETITEIRLEQNAIKVIPAGAFSPYKRLRRIDLSNNQISELASDAFQGLRSLNSLVLYGNKITEISKGLFEGLFSLQLLLLNANKIACLRVDAFQDLHNLNLLSLYDNKLQTIAKGTFSSLRAIQTLHLAQNPFICDCHLKWLADYLQDNPIETSGARCTSPRRLANKRIGQIKSKKFRCSAREQYFIPGTEDYRSKL---GGDCFADLACPEKCRCEGTTVDCSNQKLTKIPDHIPQYTAELRLNNNEFTVLEATGIFKKLPHLRKINLSNNRITDIEEGTFEGASGVNELILTSNKLENIHHSMLKGLGGLRTLMLRSNRISCVSNSSFVGLSSVRLLSLYDNQVTSMTPGAFDTLHSLSTLNLLANPFNCNCHLAWLGDWLRRKRIVTGN---PRCQNPYFLKEIPIQDVAVQDFACEDGN-----NENSCSPVMRCPAECSC-----------------LDTVVRCSNKGLTTLPKGLPKETTELYLDGNHFTQV-PVELSNYKHLTLIDLSNNQISTLSNHSLSNMSELLTLILSYNRLRCIPVRAFDGLKSLRLLSLHGNDISLIPEGAFKDLSSLSHLALGANPLYCDCHMQWLSDWVKSGYKEPGIARCAGPGDMTDKLLLTTPSKKFTCTGPV-DLSIQAKCEPCLSNPCKNDGTCTNDPVHYYRCTCPYGFKGQNCEDPIHACISNPCQNGGTCHLKEGDGSNFWCVCPEGFEGDSCEINIDDCEDNDCENNSTCVDGINNYTCMCSPEYTAATNEVERGELCEEKLDFCAPELNPCQHDSKCILTPQG-YKCECTPGFVGEHCELDYDDCKDNKCQNGAQCIDAVNGYTCVCSEGYSGLFCEFAP--PMVLP-----------------------RTSPCDNHECLNGAQCVVVGTDPR----CQCLHGYEGERCETLVSVNFVNRESYLQL--PSN-LLSPQTNISLQIATDEDSGVLLYKG-DNDHIAMELYRGRLRVSYDTGS--YPPSAIYSVETVNDGSFHAVELVAAEQTLSLSIDGGPPKSIGS--INKQSTLNIDSPLYLGGMPERAFASGGFSALQHSPGGHNGTSFHGCLRNLYINGKLQDLGAGL 1345 HSP 2 Score: 54.6842 bits (130), Expect = 1.096e-6 Identity = 42/121 (34.71%), Postives = 60/121 (49.59%), Query Frame = 1 Query: 2899 QPCRN----GGICKNIDNYGNYECVCPVGFRGPKCTINVDD-CVGNLCVNGATCNDLNN-SYSCKCQSGFRGKYCDIKFLYCNDINPCQNS----GQCQLLPNNDYKCVCSKGWEGKQCER 3231 QPC+ G C G + C C G+ G C V + C GN C+ G TC +N+ SYSC+CQ GF G CD + + +N C S G+C++ C C+ G+ G+ C+R Sbjct: 1371 QPCQRDTCVSGDCHPTGQRG-FTCTCHPGWTGTLCDQQVSNPCDGNKCIYG-TCVPINSYSYSCRCQPGFTGVLCDERDH--DTVNSCSLSHCKHGKCRVSGLGKAYCECNSGYTGEACDR 1487
BLAST of slit1 vs. Ensembl Medaka
Match: slit2 (slit guidance ligand 2 [Source:NCBI gene;Acc:101173607]) HSP 1 Score: 682.945 bits (1761), Expect = 0.000e+0 Identity = 469/1425 (32.91%), Postives = 703/1425 (49.33%), Query Frame = 1 Query: 43 MIFIFKDITFKFLLVLLSSLYCFSSTIWINDPSDESEICPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQLQ------------------------CINDNIFNKLGMLKYLLLEGNKIFWISEA-ISKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCASEDKANLENMCPSDKKV-CSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQIYNFQCN----------KEFESKYETHDEECVPKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLPPIYSIHEAPNLMKLDLSFNQIETLEPDVFTRSSK------------------------LREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQFHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLC-DGNASVYHSVDSCHETNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVLSFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYG-NYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGF--------RGKYCDIKFLYCN-DINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNSIYF-QKNSYIEISPPSRGFLMPRGNISLNFMTNSSSGLILYQGSEQTYLVVNLYQGHLRISYNLGDKVVGPEG-YSMGMVNDSRLHALHIEIVGENLTMYLNGRVHCTLKSKYLNSNRYMALDTGIFIGGAPHKILQSAIMKFHIET-----DSEFIGCMESLLINRKKIDFSKYL 4083 M+ + + L +S L TI ++ +D ++ CP C C +V+C + +VP + T RL+L N + I K+DF L +L+VLQL N+IT+IE GAF +L L +LRLNRN + P+ LF TKL +LDL +NQ+Q CI D F L L+ L L N I +S A + + L+ R+H N L C+CH+AWL+ +L+Q+ + G ++ C +P L ++ + EF C + + + S + C + C CS++ IV C GK L+ IP +LP + E+ L+ N I ++ G S YK L++I L+ N + ++ AF GL L S+ L N + I K F GL SL L L N I CL D + L+LL L +NKL ++ K TF ++ L+L NP CDC + W+ DY++D T A C PR L + ++ F+C+ +++ SK +C ++CP KC C V+CSN L +P+ I T L +++N L + P+L K++LS N+I +E F +S LR + L SNR+ C++N +F L + +L L +N ++ + +F +L L L N F+C+C L W+ +W R V GN P CQ+P LK+ PI + + F C DGN + +SC CS T V CS+ L +P +PK+TTELYLD NH + L N K L L ++LS+N+L+CI AF L +L++L L ND+S IP AF DL +L+++ALG NP +CDC+++WL+ + + + GI+ CA P M K + + F C+ D I AKC+ C+ PCK++G C+ H Y+C CPY F G+NC+ I+AC PC+NGG C + G N+ CVCP GF G C IN+DDC N C N +TC D N+Y+C C + RG+ C+ K +C ++NPCQ+ +C L P YKC C+ G+ G+ CE + DDCK N C++ +QC+D N Y C+C GY G FCE P VL S C HECLN C+ + C+C GY G C+ L S+ F + SY+++ PS L P+ NISL T+ SG++LY+G + ++ + LY+G LR+SY+ G P YS+ VND HA+ + + L++ ++G ++ S +N + +D+ +++GG P + S + + F GC+ +L IN K D L Sbjct: 1 MVGALRPAAYSRSLATVSGLLVI--TILLSSGAD-AQPCPTQCSCTGTTVDCHGQGLRSVPRNIPRNTERLDLNANNLTKITKADFAGLRHLRVLQLMENKITTIERGAFQDLKELERLRLNRNNLAVFPELLFLGTTKLHRLDLSENQIQGVPRKAFRGAVEIKNLQLDYNHISCIEDGAFRALRDLEVLTLNNNNISRLSVASFNHMPKLRTFRLHSNNLQCDCHVAWLSEWLRQRPRL---GLYTQ-CMSPPHLRGHNVAEVQKKEFVCTDAVEGHQSSSSSSCSVLQCPESCTCSNN-IVDCRGKGLTEIPTNLPETITEIRLEQNAIKVIPAGAFSPYKRLRRIDLSNNQISELASDAFQGLRSLNSLVLYGNKITEISKGLFEGLFSLQLLLLNANKIACLRVDAFQDLHNLNLLSLYDNKLQTIAKGTFSSLRAIQTLHLAQNPFICDCHLKWLADYLQDNPIETSGARCTSPRRLANKRIGQIKSKKFRCSAREQYFIPGTEDYRSKL---GGDCFADLACPEKCRCEGTTVDCSNQKLTKIPDHIPQYTAELRLNNNEFTVLEATGIFKKLPHLRKINLSNNRITDIEEGTFEGASGVNELILTSNKLENIHHSMLKGLGGLRTLMLRSNRISCVSNSSFVGLSSVRLLSLYDNQVTSMTPGAFDTLHSLSTLNLLANPFNCNCHLAWLGDWLRRKRIVTGN---PRCQNPYFLKEIPIQDVAVQDFACEDGN-----NENSCSPVMRCPAECSC-----------------LDTVVRCSNKGLTTLPKGLPKETTELYLDGNHFTQVP----VELSNYKHLTL---------------------IILSYNRLRCIPVRAFDGLKSLRLLSLHGNDISLIPEGAFKDLSSLSHLALGANPLYCDCHMQWLSDWVKSGYKEPGIARCAGPGDMTDKLLLTTPSKKFTCTGPV-DLSIQAKCEPCLSNPCKNDGTCTNDPVHYYRCTCPYGFKGQNCEDPIHACISNPCQNGGTCHLKEGDGSNFWCVCPEGFEGDSCEINIDDCEDNDCENNSTCVDGINNYTCMCSPEYTAATNEVERGELCEEKLDFCAPELNPCQHDSKCILTPQG-YKCECTPGFVGEHCELDYDDCKDNKCQNGAQCIDAVNGYTCVCSEGYSGLFCEFAP--PMVLP-----------------------RTSPCDNHECLNGAQCVVVGTDPR----CQCLHGYEGERCETLVSVNFVNRESYLQL--PSN-LLSPQTNISLQIATDEDSGVLLYKG-DNDHIAMELYRGRLRVSYDTGS--YPPSAIYSVETVNDGSFHAVELVAAEQTLSLSIDGGPPKSIGS--INKQSTLNIDSPLYLGGMPERAFASGGFSALQHSPGGHNGTSFHGCLRNLYINGKLQDLGAGL 1325 HSP 2 Score: 54.299 bits (129), Expect = 1.317e-6 Identity = 42/121 (34.71%), Postives = 60/121 (49.59%), Query Frame = 1 Query: 2899 QPCRN----GGICKNIDNYGNYECVCPVGFRGPKCTINVDD-CVGNLCVNGATCNDLNN-SYSCKCQSGFRGKYCDIKFLYCNDINPCQNS----GQCQLLPNNDYKCVCSKGWEGKQCER 3231 QPC+ G C G + C C G+ G C V + C GN C+ G TC +N+ SYSC+CQ GF G CD + + +N C S G+C++ C C+ G+ G+ C+R Sbjct: 1351 QPCQRDTCVSGDCHPTGQRG-FTCTCHPGWTGTLCDQQVSNPCDGNKCIYG-TCVPINSYSYSCRCQPGFTGVLCDERDH--DTVNSCSLSHCKHGKCRVSGLGKAYCECNSGYTGEACDR 1467
BLAST of slit1 vs. Ensembl Medaka
Match: slit1a (slit guidance ligand 1 [Source:NCBI gene;Acc:101164917]) HSP 1 Score: 679.478 bits (1752), Expect = 0.000e+0 Identity = 481/1583 (30.39%), Postives = 752/1583 (47.50%), Query Frame = 1 Query: 148 SEICPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQLQ------------------------CINDNIFNKLGMLKYLLLEGNKIFWIS-EAISKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCASEDKANLENMCPSDKKVCSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQIYNFQCNKEFE--SKYETHD----EECVPKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLPPIYSIHEAPNLMKLDLSFNQIETLEPDVFTRSSKLREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQ------------------------FHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCDGNASVYHSVDSCHETNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVLSFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICK-NIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYC-DIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNSIYF-QKNSYIEISPPSRGFLMPRGNISLNFMTNSSSGLILYQGSEQTYLVVNLYQGHLRISYNLGDKVVGPEGYSMGMVNDSRLHALHIEIVGENLTMYLNGRVHCTLKSKYLNSNRYMALDTGIFIGGAPHKILQSAIMKFHIETDSEFIGCMESLLINRKKIDFSKYLPKSINVHPGCGKLISTVVASDLKIPSIMXXXXXXXXXXXXXXXNNVCKRPGICKNQGV----CEVLPGSKKKFRCICKNGFVGRKCKKRKSM-------------------------CHGTFTESHIVDPDSQGTCKSIERFQYRICTG--TCSTSRMQSDVIKNFYHKPIDPSSSRCCQPTKYSTHEIQFKCENGRVYRRAANLPKNCKCVA 4629 + CP C C +V+C +P + T RL L GN + I KSDF L ++VLQL N+IT +E GAFD++ L +LRLNRN +Q LP+ LF K L +LDL +N +Q CI D F + L+ L L N I I + + + L+ R+H N LNC+CHLAWL +L+Q+ I C+ P L + ++ + +EF C+ +++ C C C CS++ IV C GK L+ IP +LP N+ E+ L+ N I + G S YK L++I L+ N + +I P AF GL L S+ L N + + K F GL +L L L N I C+ +T + + L LL L +NK+ +L K TF ++ L+L NP CDC + W+ DY++ T A C PR L + ++ F+C+ S T D + C CP KC C S+VV+CSN L +PE I ST L +++N + L + L K++LS N+I +E F +S + E+ L +N++ + + F+ L+ L +L L N ISC+ DSF N++ L L NQ F+CDC+L W+ +W R+ V GN P CQ P LK+ P+ + F CD SC + C+ T V CS+ L +P IP++ TELYLD N S+ L+ + LS N I L + + L TL+LS+N L+CI + AF LH+L++L L N++S +P FND+ +L+++A+G NP HCDC ++WL+ + + + GI+ CA P+ ++ K + + F C + D + +KC+ C+ PC ++G C Y+C CP F GKNC+ +NAC PC NGG C+ + DN G Y C C +GF GP C IN DDC N C NGATC D N+Y+C C + G+ C +++ + +PCQ+ C L+ ++ KCVCS G+ G C + +DC+ + C++ +QCVD + Y C+C G+ G+ CE+P P+ L S+C +C N+ C+ + ++ C+C P + G C+ L S+ F ++SY+ +S P+ NI+L T +G++LY G + ++ V L+QGH+++SY+ G + G YS +ND + H + + + + + ++G + T+ S + R + + +++GG P + + I+ S F GC+++L IN + DF+K K V PGC + P Q C +P + QG + +P + +RC C+ G+ G C ++ + C +T + D +S+ + + F Y++ G C T+RM S + S+ CC + + F+C +G + C CV Sbjct: 33 ASACPALCTCSGVTVDCHGLGLKTMPRNIPRNTERLELNGNNLTRITKSDFTGLKYVRVLQLMENQITVVERGAFDDMKELERLRLNRNQLQQLPELLFQKNPALSRLDLSENFIQSIPRKAFRGATDIKNLQLDKNHISCIEDGAFRAMRGLEVLTLNNNNISSIPVSSFNHMPKLRTFRLHSNNLNCDCHLAWLAQWLRQRPTI----GLFTQCTVPSELKGLNVAEVQKHEFTCSGHQESSSLQPCSIGGGSCPAMCTCSNN-IVDCRGKGLTAIPANLPDNMAEIRLEQNGIKSVPPGAFSSYKKLRRIDLSNNQISEIAPDAFQGLRSLNSLVLYGNKITDLPKGVFDGLYALQLLLLNANKIHCIRANTFQDLQNLSLLSLYDNKIQTLAKGTFTSLRAIQTLHLAQNPFICDCNLKWLADYLRSNPIETSGARCASPRRLANKRIGQIKSKKFRCSGSILTFSPLGTEDVRLNDACNSDPVCPPKCRCESNVVDCSNLKLTKIPEHIPSSTSELRLNNNEITSLDANGAFKNLAQLKKINLSNNKITEIEDGTFEGASAVIELHLTANQIDSVRSGMFRGLEGLRMLMLRNNKISCVHNDSFTGLHNVRLLSLYDNQLTTITPGAFDTLQTLSTLNLLANSFNCDCRLAWLGDWLRSRKIVTGN---PRCQRPAFLKEIPLQDVALPDFRCDEG----QEESSCIPRPQCPSECTC-----------------LETVVRCSNKHLHSLPRGIPRNVTELYLDGNQF-SIVPKELSTFKYLQLVDLSNNKINSLTNSSFANMSQLTTLILSYNSLRCIPKMAFSGLHSLRLLSLHGNEISELPDGIFNDVASLSHLAIGANPLHCDCRLRWLSDWVKTGYKEPGIARCAGPQGLEGKLLLTTPAKKFECHGDV-DIAVLSKCNPCLSNPCMNHGICHNDPVEIYRCSCPPGFKGKNCESGLNACVSNPCANGGTCRVHEDNEGQYSCTCALGFEGPTCKINTDDCEDNDCENGATCVDGINNYTCFCPPYYTGEMCEEMEDVCAPGRSPCQHQSTC-LITSSGPKCVCSPGYVGDDCSIDYNDCEEHRCQNGAQCVDELSGYSCICPKGFSGQLCEVP---PSPL--------------------------SLCELADCQNDAPCV----ERGGRALCQCPPEFGGPRCEKLVSVNFIDRDSYLLLSDLKN---WPQANITLQVSTAEDNGILLYNG-DNDHIAVELFQGHVKVSYDPGSQ-PGHAIYSTETINDGQFHTVELVTFDQMVNLSIDGGLPTTMDS--FGAVRPLNREAPLYVGGMPVDVHSGTFRLWQIQNSSSFHGCIQNLYINNELQDFTKTQMKP-GVVPGCEPCKKLYCVHGICQPDGAQGPVCHCQPGWIGAH---CDQPALNPCQGSKCVHGKCIPLDAQSYRCECQEGYRGALCNQQGELFNPCRRLSCKHGRCQISDAGSAYCHCESGYT-GELCDAESECRGEPVRDF-YQVQRGYAICQTTRMVS-----WVECSGACSTGACCGSQRMKRRKYTFECSDGTSFSEEVEKTIKCGCVG 1532 HSP 2 Score: 188.348 bits (477), Expect = 1.806e-47 Identity = 135/453 (29.80%), Postives = 226/453 (49.89%), Query Frame = 1 Query: 121 IWINDPSDESEICPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLK-LNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQLQCINDNIFNKLGMLKYLLLEGNKIFWISE-AISKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCASEDKANLENMCPSDKKVCSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQIYNFQCNKEFE 1473 + +ND + +CP C+CE+ V+C + +P + T+ L L N I + + K L LK + LSNN+IT IE G F+ +I+L L N I + +F L L+ L LR+N++ C++++ F L ++ L L N++ I+ A L+ L L + N NC+C LAWL +L+ + + + C P L IP+ ++ +F+C D+ E+ C + C +C C + +V+C K L ++P+ +P NV EL L N +I+ + LS +K+L+ + L+ N + + +F ++QLT++ L+ N+L+ I K AF GL SL L L N+I LP F +L +L + NPL+CDCR+ W+ D++K +A C P+ L+G L F+C+ + + Sbjct: 504 VRLNDACNSDPVCPPKCRCESNVVDCSNLKLTKIPEHIPSSTSELRLNNNEITSLDANGAFKNLAQLKKINLSNNKITEIEDGTFEGASAVIELHLTANQIDSVRSGMFRGLEGLRMLMLRNNKISCVHNDSFTGLHNVRLLSLYDNQLTTITPGAFDTLQTLSTLNLLANSFNCDCRLAWLGDWLRSRKIVTG----NPRCQRPAFLKEIPLQDVALPDFRC---DEGQEESSC-IPRPQCPSECTC-LETVVRCSNKHLHSLPRGIPRNVTELYLDGNQFSIVPK-ELSTFKYLQLVDLSNNKINSLTNSSFANMSQLTTLILSYNSLRCIPKMAFSGLHSLRLLSLHGNEISELPDG------------------------IFNDVASLSHLAIGANPLHCDCRLRWLSDWVKTGYKEPGIARCAGPQGLEGKLLLTTPAKKFECHGDVD 922
BLAST of slit1 vs. Planmine SMEST
Match: SMESG000068721.1 (SMESG000068721.1) HSP 1 Score: 2980.27 bits (7725), Expect = 0.000e+0 Identity = 1531/1534 (99.80%), Postives = 1532/1534 (99.87%), Query Frame = 1 Query: 43 MIFIFKDITFKFLLVLLSSLYCFSSTIWINDPSDESEICPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDXXXXXXXXXXXXXXXQYLPDDLFSKLTKLQKLDLRDNQLQCINDNIFNKLGMLKYLLLEGNKIFWISEAISKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCASEDKANLENMCPSDKKVCSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQIYNFQCNKEFESKYETHDEECVPKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLPPIYSIHEAPNLMKLDLSFNQIETLEPDVFTRSSKLREISLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFXXXXXXXXXXXXXXQFHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCDGNASVYHSVDSCHETNSVKTCCSIGNXXXXXXXXXXXXXXXXXXXVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVLSFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNSIYFQKNSYIEISPPSRGFLMPRGNISLNFMTNSSSGLILYQGSEQTYLVVNLYQGHLRISYNLGDKVVGPEGYSMGMVNDSRLHALHIEIVGENLTMYLNGRVHCTLKSKYLNSNRYMALDTGIFIGGAPHKILQSAIMKFHIETDSEFIGCMESLLINRKKIDFSKYLPKSINVHPGCGKLISTVVASDLKIPSIMXXXXXXXXXXXXXXXNNVCKRPGICKNQGVCEVLPGSKKKFRCICKNGFVGRKCKKRKSMCHGTFTESHIVDPDSQGTCKSIERFQYRICTGTCSTSRMQSDVIKNFYHKPIDPSSSRCCQPTKYSTHEIQFKCENGRVYRRAANLPKNCKCVANCLND 4644 MIFIFKDITFKFLLVLLSSLYCFSSTIWINDPSDESEICPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDNLVNLIKLRLNRNLIQYLPDDLFSKLTKLQKLDLRDNQLQCINDNIFNKLGMLKYLLLEGNKIFWISEAISKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCASEDKANLENMCPSDKKVCSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQIYNFQCNKEFESKYETHDEECVPKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKL PIYSIHEAPNLMKLDLSFNQIETLEPDVFTRSSKLREI+LNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFKNNLNLKYLMLNKNQFHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCDGNASV HSVDSCHETNSVKTCCSIGNEECTKSKKCPSKCKCESTKVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVLSFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNSIYFQKNSYIEISPPSRGFLMPRGNISLNFMTNSSSGLILYQGSEQTYLVVNLYQGHLRISYNLGDKVVGPEGYSMGMVNDSRLHALHIEIVGENLTMYLNGRVHCTLKSKYLNSNRYMALDTGIFIGGAPHKILQSAIMKFHIETDSEFIGCMESLLINRKKIDFSKYLPKSINVHPGCGKLISTVVASDLKIPSIMQPIKQILTQTTKKKPNNVCKRPGICKNQGVCEVLPGSKKKFRCICKNGFVGRKCKKRKSMCHGTFTESHIVDPDSQGTCKSIERFQYRICTGTCSTSRMQSDVIKNFYHKPIDPSSSRCCQPTKYSTHEIQFKCENGRVYRRAANLPKNCKCVANCLND Sbjct: 1 MIFIFKDITFKFLLVLLSSLYCFSSTIWINDPSDESEICPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDNLVNLIKLRLNRNLIQYLPDDLFSKLTKLQKLDLRDNQLQCINDNIFNKLGMLKYLLLEGNKIFWISEAISKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCASEDKANLENMCPSDKKVCSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQIYNFQCNKEFESKYETHDEECVPKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLTPIYSIHEAPNLMKLDLSFNQIETLEPDVFTRSSKLREINLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFKNNLNLKYLMLNKNQFHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCDGNASVDHSVDSCHETNSVKTCCSIGNEECTKSKKCPSKCKCESTKVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVLSFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNSIYFQKNSYIEISPPSRGFLMPRGNISLNFMTNSSSGLILYQGSEQTYLVVNLYQGHLRISYNLGDKVVGPEGYSMGMVNDSRLHALHIEIVGENLTMYLNGRVHCTLKSKYLNSNRYMALDTGIFIGGAPHKILQSAIMKFHIETDSEFIGCMESLLINRKKIDFSKYLPKSINVHPGCGKLISTVVASDLKIPSIMQPIKQILTQTTKKKPNNVCKRPGICKNQGVCEVLPGSKKKFRCICKNGFVGRKCKKRKSMCHGTFTESHIVDPDSQGTCKSIERFQYRICTGTCSTSRMQSDVIKNFYHKPIDPSSSRCCQPTKYSTHEIQFKCENGRVYRRAANLPKNCKCVANCLND 1534
BLAST of slit1 vs. Planmine SMEST
Match: SMESG000063217.1 (SMESG000063217.1) HSP 1 Score: 135.191 bits (339), Expect = 2.602e-31 Identity = 84/246 (34.15%), Postives = 117/246 (47.56%), Query Frame = 1 Query: 2722 ICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQN--------------------------------------SGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEI 3345 IC E D+C K C + G C + +C+C F G NC+ +N C +PC+N C +++N +Y C CP G G C IN+D+C N C+NGA C D ++C+C GF G C+ K C + PC+ +GQC + NN+ KC+C G+EG+ CE ++C N C+H SQC DL NDY C+C PG+ GK CEI Sbjct: 316 ICQRGFEGKRCETSIDNCQKISCFNGGSCINMIEGG-KCNCLPGFEGNNCEINVNECENKPCQNNAKCIDLNN--DYTCDCPEGLTGKNCEINIDECTPNPCLNGARCEDRVGGFTCQCLQGFVGLRCETKSDICKQLTPCKTEEICLNVNNTRSGYKCICPDGVQNCKKELSKCKNIVCVNGQCVDV-NNEEKCICIPGFEGRTCEIEINECFNNPCQHGSQCQDLFNDYLCICLPGFKGKKCEI 557 HSP 2 Score: 126.716 bits (317), Expect = 9.637e-29 Identity = 73/186 (39.25%), Postives = 103/186 (55.38%), Query Frame = 1 Query: 2788 CKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEI 3345 C++ G C + T +YQC C F+G+ C+ I C C+NGG C +D G+Y CVCP + G C C C NG C D + Y C C + + GK CD C INPC+N+G C+ + DYKC+C +G+EGK+CE + D+C+ SC + C+++ KC C PG+ G CEI Sbjct: 185 CENGGTC-INTVGSYQCKCKAGFNGERCENDIVDCTNDTCKNGGTC--LDKIGSYRCVCPNNYFGTNCENKRTLCELKPCKNGGICRDFDEDYQCICGNQWTGKNCDDVKNPC-KINPCKNNGFCESVNYQDYKCICQRGFEGKRCETSIDNCQKISCFNGGSCINMIEGGKCNCLPGFEGNNCEI 366 HSP 3 Score: 120.939 bits (302), Expect = 5.523e-27 Identity = 87/298 (29.19%), Postives = 144/298 (48.32%), Query Frame = 1 Query: 2767 DSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVG---FRGPKCTINVDDC---------------------VGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAY-------HECLNNGICINGDQNKNQKSY-CKCKPGYSGHLCQ 3564 + C PC NG+C + + ++C C P+ G C +++ C C N IC + Y +Y C C + G KC +VD+C +G C+N C + N S+ CKC S + G+ C+ C + +PC+N C L + ++C C G+ G++CE ++C++N C ++++C+D NDY+C+C+ G+ GK CEI T L + +++ +E NE + C + + CLN G CIN N+N Y C C+ G++G C+ Sbjct: 560 NECAHNPCGLNGQC-IDEVYGFRCQCMLPYSGPTCANKLDPCNPNACYNDAICIPGNKYDSYHCNCSTALGRYEGSKCEKDVDECNLDRTQLALYGLALLSMSNSQIG-PCLNDNQCINTNGSFICKCSSQYEGRQCEKPLNNCRN-DPCRNGATCDNLADG-FRCRCVAGFTGERCEIEINECQHNPCSNDAKCIDHINDYQCICQSGFSGKNCEINDNDCTESSCLGHGQCVDKVN-GFECKCNENYIGTNCEHLKRPCDSNPCLNKGSCINRKVNQNTPGYLCNCRRGFTGPRCE 852 HSP 4 Score: 118.627 bits (296), Expect = 2.933e-26 Identity = 87/286 (30.42%), Postives = 123/286 (43.01%), Query Frame = 1 Query: 2725 CSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQC------QLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQ 3564 CS + E ++C PC++ C + ++C C F G+ C+ IN C PC N C ID+ +Y+C+C GF G C IN +DC + C+ C D N + CKC + G C+ C D NPC N G C Q P Y C C +G+ G +CE D C+ N C C + +KC C YIG+ CEI ++ E +NE + +C +H G C N + K C C+ GY G C+ Sbjct: 685 CSSQYEGRQCEKPLNNCRNDPCRNGATCDNLA-DGFRCRCVAGFTGERCEIEINECQHNPCSNDAKC--IDHINDYQCICQSGFSGKNCEINDNDCTESSCLGHGQCVDKVNGFECKCNENYIGTNCEHLKRPC-DSNPCLNKGSCINRKVNQNTPG--YLCNCRRGFTGPRCEIKMDWCRGNPCGDFGVCETDLDSFKCKCNSEYIGERCEI-------------KKTETCKSYAVEKFKNESLINKICKHH-----GNCFNDPETGGFK--CSCQSGYGGSFCE 944 HSP 5 Score: 117.472 bits (293), Expect = 6.161e-26 Identity = 93/314 (29.62%), Postives = 131/314 (41.72%), Query Frame = 1 Query: 2773 CMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQP-------------------------------------------CRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDL-HNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENL------ENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQ 3564 C PC++ G C + H Y+C CP + G NC+ N+C Q C NGG C I+ G+Y+C C GF G +C ++ DC + C NG TC D SY C C + + G C+ K C ++ PC+N G C+ + DY+C+C W GK C+ + CK N CK+ C + + DYKC+C+ G+ GK CE + IN E N N I+ + C C NN CI + N C C G +G C+ Sbjct: 99 CASSPCRNGGICKSLEIHKYECQCPKNYLGMNCE-LPNSCGSQNPCGVNGKCILANDGEVKCHCNKGYTGDTCSNDIDECASYETPLCENGGTC--INTVGSYQCKCKAGFNGERCENDIVDCTNDTCKNGGTCLDKIGSYRCVCPNNYFGTNCENKRTLC-ELKPCKNGGICRDF-DEDYQCICGNQWTGKNCDDVKNPCKINPCKNNGFCESVNYQDYKCICQRGFEGKRCETSIDNCQKISCFNGGSCINMIEGGKCNCLPGFEGNNCEINVNECENKPCQNNAKCI----DLNNDYTCDCPEGLTGKNCE 403 HSP 6 Score: 115.161 bits (287), Expect = 3.589e-25 Identity = 90/302 (29.80%), Postives = 136/302 (45.03%), Query Frame = 1 Query: 2767 DSCMKRPCKHNGKC-SLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHES----------------QCVDLHNDYKCMCRPGYIGKFCE--------------IPAWKPTVLKTLISR---RDINQSEMTYENLENEV--ISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQ 3564 +S + + CKH+G C + ++C C + G C+ I+ C QPC NGG C D G Y C C GF+G C +N ++C N C N C D N++SC C G G++C+ YC + CQN G C+ + D++C C KG+ GK CE + ++CKYN + + +C+D + C C+ + GK+CE IP + + R+ N S + E I+ C + C NNGIC + + C+C PG G C+ Sbjct: 908 ESLINKICKHHGNCFNDPETGGFKCSCQSGYGGSFCEVNIDDCASQPCINGGECG--DGTGMYFCKCQPGFQGVNCQVNTNECYSNSC-NTGKCIDQINNFSCVCPRGVSGRFCETIVDYCKKME-CQNGGICKNVIG-DFECNCPKGFAGKLCETDINECKYNIAGNATNLISNPHGPCSPTGTLECLDRPGTFHCSCKKYFTGKYCETRIDYCEKFRKSGNIPCFNNGICLNKYQNEVGRNYNFSCICPNGYGGERCKINSKKCQLNVCKNNGICRPFEIDDRMSFKCECPPGVGGLFCE 1204 HSP 7 Score: 111.309 bits (277), Expect = 4.656e-24 Identity = 86/290 (29.66%), Postives = 128/290 (44.14%), Query Frame = 1 Query: 2800 GKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGAT----------------CNDLNNSYSCKCQSGFRGKYCDIKFLYCNDIN-----PCQNSGQC------QLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQC----VDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNS 3576 GKC + +N+ C CP G+ C+ ++ C C+NGGICKN+ G++EC CP GF G C ++++C N+ N C D ++ C C+ F GKYC+ + YC PC N+G C ++ N ++ C+C G+ G++C+ N+ C+ N CK+ C +D +KC C PG G FCEI + NE +S +C NNG C+NG K+ C C G G C+ S Sbjct: 996 GKC-IDQINNFSCVCPRGVSGRFCETIVDYCKKMECQNGGICKNV--IGDFECNCPKGFAGKLCETDINECKYNIAGNATNLISNPHGPCSPTGTLECLDRPGTFHCSCKKYFTGKYCETRIDYCEKFRKSGNIPCFNNGICLNKYQNEVGRNYNFSCICPNGYGGERCKINSKKCQLNVCKNNGICRPFEIDDRMSFKCECPPGVGGLFCEI-------------------------DYVNECLST------KCYNNGSCVNGF----GKAICHCNKGSCGEKCEITGS 1247 HSP 8 Score: 105.531 bits (262), Expect = 3.232e-22 Identity = 94/313 (30.03%), Postives = 134/313 (42.81%), Query Frame = 1 Query: 2761 KCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCD-KRINAC-------------FGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCE--IPAWKPTV---LKTLISRRDINQSEM-TYENLENEVISESVCAYH----------------------ECLNNGICINGDQNKNQKSY---CKCKPGYSGHLCQ 3564 K D C PC G C +++C C + G+ C+ K+ C + C++ G C N G ++C C G+ G C +N+DDC C+NG C D Y CKCQ GF+G C + C N C N+G+C + N++ CVC +G G+ CE D CK C++ C ++ D++C C G+ GK CE I K + LIS S T E L+ C + C NNGIC+N QN+ ++Y C C GY G C+ Sbjct: 854 KMDWCRGNPCGDFGVCE-TDLDSFKCKCNSEYIGERCEIKKTETCKSYAVEKFKNESLINKICKHHGNCFNDPETGGFKCSCQSGYGGSFCEVNIDDCASQPCINGGECGDGTGMYFCKCQPGFQGVNCQVNTNECYS-NSC-NTGKC-IDQINNFSCVCPRGVSGRFCETIVDYCKKMECQNGGICKNVIGDFECNCPKGFAGKLCETDINECKYNIAGNATNLISNPHGPCSPTGTLECLDRPGTFHCSCKKYFTGKYCETRIDYCEKFRKSGNIPCFNNGICLNKYQNEVGRNYNFSCICPNGYGGERCK 1162 HSP 9 Score: 98.9821 bits (245), Expect = 3.009e-20 Identity = 78/258 (30.23%), Postives = 121/258 (46.90%), Query Frame = 1 Query: 2773 CMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLN-NSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDC-KYNS--CKHESQCVDLHNDYKCMCRPGYIGKFCE---------IPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQN 3507 C CK+ C + Y C+C Y + G C+++ N C PC+NGG CK I GNY C C + G C + +C + C NG C L + Y C+C + G C++ C NPC +G+C L + + KC C+KG+ G C + D+C Y + C++ C++ Y+C C+ G+ G+ CE T L + S R + + N EN+ ++C C N GIC + D++ Sbjct: 24 CENHKCKNGATCQSDINGKYVCECAYGYKGTYCEQKSN-CPITPCQNGGTCKLI--AGNYVCQCLNEWVGEFCEFS-SNCASSPCRNGGICKSLEIHKYECQCPKNYLGMNCELPN-SCGSQNPCGVNGKCILANDGEVKCHCNKGYTGDTCSNDIDECASYETPLCENGGTCINTVGSYQCKCKAGFNGERCENDIVDCTNDTCKNGGTCLDKIGSYRCVCPNNYFGTNCENK---RTLCELKPCKNGGICRDFDED 273 HSP 10 Score: 91.2781 bits (225), Expect = 6.180e-18 Identity = 81/267 (30.34%), Postives = 123/267 (46.07%), Query Frame = 1 Query: 2785 PCKHNGKCSLI--THHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCV--DLHNDYKCMCRPG---YIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQ 3564 PCK C + T Y+C CP +NC K ++ C C NG C +++N +C+C GF G C I +++C N C +G+ C DL N Y C C GF+GK C+I C NPC +GQC + ++C C + G C D C N+C +++ C+ + ++ Y C C Y G CE + +++++++ L ++S S CLN+ CI N N CKC Y G C+ Sbjct: 452 PCKTEEICLNVNNTRSGYKCICPDGV--QNCKKELSKCKNIVCVNGQ-CVDVNN--EEKCICIPGFEGRTCEIEINECFNNPCQHGSQCQDLFNDYLCICLPGFKGKKCEININECAH-NPCGLNGQC-IDEVYGFRCQCMLPYSGPTCANKLDPCNPNACYNDAICIPGNKYDSYHCNCSTALGRYEGSKCE----------KDVDECNLDRTQLALYGL--ALLSMSNSQIGPCLNDNQCI----NTNGSFICKCSSQYEGRQCE 695 HSP 11 Score: 65.4698 bits (158), Expect = 4.358e-10 Identity = 52/158 (32.91%), Postives = 73/158 (46.20%), Query Frame = 1 Query: 2830 YQCDCPYPFHGKNCDKRINAC--FGQ----PCRNGGICKN-----IDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCN----DLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNS 3258 + C C F GK C+ RI+ C F + PC N GIC N + N+ C+CP G+ G +C IN C N+C N C D S+ C+C G G +C+I ++ C N+G C + C C+KG G++CE C S Sbjct: 1097 FHCSCKKYFTGKYCETRIDYCEKFRKSGNIPCFNNGICLNKYQNEVGRNYNFSCICPNGYGGERCKINSKKCQLNVCKNNGICRPFEIDDRMSFKCECPPGVGGLFCEIDYVNECLSTKCYNNGSC-VNGFGKAICHCNKGSCGEKCEITGSACNLAS 1253 HSP 12 Score: 56.6102 bits (135), Expect = 1.980e-7 Identity = 47/158 (29.75%), Postives = 71/158 (44.94%), Query Frame = 1 Query: 2707 KPTDFICS--EETEDAYITAKCDSCMKR------PCKHNGKC------SLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICK--NIDNYGNYECVCPVGFRGPKCTIN-VDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCN 3129 +P F CS + Y + D C K PC +NG C + ++N+ C CP + G+ C C C+N GIC+ ID+ +++C CP G G C I+ V++C+ C N +C + C C G G+ C+I CN Sbjct: 1093 RPGTFHCSCKKYFTGKYCETRIDYCEKFRKSGNIPCFNNGICLNKYQNEVGRNYNFSCICPNGYGGERCKINSKKCQLNVCKNNGICRPFEIDDRMSFKCECPPGVGGLFCEIDYVNECLSTKCYNNGSCVNGFGKAICHCNKGSCGEKCEITGSACN 1250
BLAST of slit1 vs. Planmine SMEST
Match: SMESG000027402.1 (SMESG000027402.1) HSP 1 Score: 133.265 bits (334), Expect = 7.828e-31 Identity = 105/355 (29.58%), Postives = 152/355 (42.82%), Query Frame = 1 Query: 2722 ICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDI----------------------NPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENE-----------VISESVCAYHECLNNGIC-----INGDQNKNQKSYCKCKPGYSGHLCQYLNSIYFQKNSYIEISPPSRGFLMPRGNISLNFMTN 3672 +C Y + + C+ PCK+NG C+ + ++Y+C+C F GKNC+ I AC QPC+NGG+C+ +D Y C C G+ G C I C N C+NGA C +N + C C F GK C+ YC + N C G C + C C G+EG CE + DDCK N CK+ C+D N+++C+C G+ GK CE + + S+ Y + E + S+C + CLN G C +NG Q C C G+ G +CQ+L +I+ P R L + +N N Sbjct: 439 LCQPGYTGKYCDKEINECVPNPCKNNGVCT-DSINDYKCNCSTSFKGKNCELNI-ACQNQPCKNGGVCQEVDT--GYTCKCKTGYTGTNCEIYKTGCSPNPCLNGAYCYTINQDFYCHCNKEFWGKRCEHSRPYCGNESCESAYDACSMPQMLNSSGIKHNYCGKYGTCLSHEKEKFICRCMPGFEGDSCEIDIDDCKTNPCKNGGICIDSINNFECICDNGFFGKTCEERTDYCIKSPCMNYGVCLRHSDELYNDFICECKNNWGGRLCHLPKHSICDSNPCLNGGSCSRLSTLNGFQ-------CHCNQGWIGTVCQFL---------HIDFQPCRRKNLCKNEGVCINVGYN 773 HSP 2 Score: 112.079 bits (279), Expect = 2.340e-24 Identity = 70/201 (34.83%), Postives = 106/201 (52.74%), Query Frame = 1 Query: 2767 DSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDN--YGNYECVCPVGFRGPKCTINVDD-CVGNLCVNGATCNDLN--NSYSCKCQSGFRGKYCD---IKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEI 3345 D C PCK+ G C + + +N++C C F GK C++R + C PC N G+C + Y ++ C C + G C + C N C+NG +C+ L+ N + C C G+ G C I F C N C+N G C + ND+ C C G+EGK C+ NT++C N C + +C+D N Y+C+C G+ G C++ Sbjct: 629 DDCKTNPCKNGGIC-IDSINNFECICDNGFFGKTCEERTDYCIKSPCMNYGVCLRHSDELYNDFICECKNNWGGRLCHLPKHSICDSNPCLNGGSCSRLSTLNGFQCHCNQGWIGTVCQFLHIDFQPCRRKNLCKNEGVCINVGYNDFTCHCKPGFEGKHCDINTNECNPNPCFNGGKCIDKLNSYRCICAKGFYGSNCQL 828 HSP 3 Score: 111.694 bits (278), Expect = 2.680e-24 Identity = 101/319 (31.66%), Postives = 135/319 (42.32%), Query Frame = 1 Query: 2764 CDSCMKRP-CKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACF-GQPCRNGGICKNI-DNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQC----------------------------------------QLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEI-------PAWKPTVLKTLISRRDIN-QSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQY 3567 CD CMK P CKH G C + C C + G+ CD+ +N C PC+NGGIC+N+ Y+C+CP GF G C I D C N C G TC + C+C + GK CD C D N CQN GQC Q DYKC+C G+ GK C++ ++C N CK+ C D NDYKC C + GK CE+ P V + + + ++ T N E I ++ C+ + CLN C NQ YC C + G C++ Sbjct: 261 CDECMKYPGCKH-GTC---VDSPWTCSCENNWGGQFCDQSLNYCAENNPCKNGGICENLLSEVQPYKCICPSGFEGRDCEI--DPCFPNPCKKG-TCMKSKGEFYCQCIERWTGKLCDQDTSIC-DQNSCQNGGQCGKDENGNRRCICQQGYKGSECHLAAPCENSQCKHALDCQIQSTALGDYKCLCQPGYTGKYCDKEINECVPNPCKNNGVCTDSINDYKCNCSTSFKGKNCELNIACQNQPCKNGGVCQEVDTGYTCKCKTGYTGTNCE---IYKTGCSPNPCLNGAYCY----TINQDFYCHCNKEFWGKRCEH 564 HSP 4 Score: 110.923 bits (276), Expect = 4.650e-24 Identity = 86/280 (30.71%), Postives = 132/280 (47.14%), Query Frame = 1 Query: 2788 CKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATC----NDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPN-NDYKCVCSKGWEGKQCERNTDDC----KYNSCKHESQCVDL-HNDYKCMCRPGYIGKFCEI--------PAWKP-TVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYL 3570 C G C + C C F G +C+ I+ C PC+NGGIC ID+ N+EC+C GF G C D C+ + C+N C ++L N + C+C++ + G+ C + D NPC N G C L N ++C C++GW G C+ D + N CK+E C+++ +ND+ C C+PG+ GK C+I P + + L S R I N + ++ CA C IC++ + C+C G G LC ++ Sbjct: 597 CGKYGTCLSHEKEKFICRCMPGFEGDSCEIDIDDCKTNPCKNGGIC--IDSINNFECICDNGFFGKTCEERTDYCIKSPCMNYGVCLRHSDELYNDFICECKNNWGGRLCHLPKHSICDSNPCLNGGSCSRLSTLNGFQCHCNQGWIGTVCQFLHIDFQPCRRKNLCKNEGVCINVGYNDFTCHCKPGFEGKHCDINTNECNPNPCFNGGKCIDKLNSYRCICAKGFYGSNCQ---LNYDECASFPCAYGAICVD----RIDDFECRCPEGRDGKLCDHV 867 HSP 5 Score: 105.145 bits (261), Expect = 3.302e-22 Identity = 71/201 (35.32%), Postives = 107/201 (53.23%), Query Frame = 1 Query: 2767 DSCMKRPCKHNGKC---SLITHHNYQCDCPYPFHGKNCD-KRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCT---INVDDCV-GNLCVNGATCNDLN-NSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCE 3342 D C+K PC + G C S ++++ C+C + G+ C + + C PC NGG C + ++C C G+ G C I+ C NLC N C ++ N ++C C+ GF GK+CDI CN NPC N G+C + N Y+C+C+KG+ G C+ N D+C C + + CVD +D++C C G GK C+ Sbjct: 667 DYCIKSPCMNYGVCLRHSDELYNDFICECKNNWGGRLCHLPKHSICDSNPCLNGGSCSRLSTLNGFQCHCNQGWIGTVCQFLHIDFQPCRRKNLCKNEGVCINVGYNDFTCHCKPGFEGKHCDINTNECNP-NPCFNGGKC-IDKLNSYRCICAKGFYGSNCQLNYDECASFPCAYGAICVDRIDDFECRCPEGRDGKLCD 865 HSP 6 Score: 88.9669 bits (219), Expect = 2.819e-17 Identity = 92/358 (25.70%), Postives = 148/358 (41.34%), Query Frame = 1 Query: 2626 FLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKCD---SCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKC-----------------------TINVDDCVGNLCVNGATC-NDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCV----DLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMT----YENLENEVISESVCAY-----------HECLNNGICINGDQNKNQKSYCKCKPGYSGHLC 3561 + ++ D I+ C P K + D+ C+ T ++ C+ +C +PCK+ G C + Y C C + G NC+ C PC NG C I+ ++ C C F G +C +N N C TC + + C+C GF G C+I C NPC+N G C + N+++C+C G+ GK CE TD C + C + C+ +L+ND+ C C+ + G+ C +P K ++ + + S ++ ++ N+ +VC + + C N G+CIN N C CKPG+ G C Sbjct: 444 YTGKYCDKEINECV-PNPCKNNGVCTDSINDYKCNCST--SFKGKNCELNIACQNQPCKNGGVCQEVDT-GYTCKCKTGYTGTNCEIYKTGCSPNPCLNGAYCYTINQ--DFYCHCNKEFWGKRCEHSRPYCGNESCESAYDACSMPQMLNSSGIKHNYCGKYGTCLSHEKEKFICRCMPGFEGDSCEIDIDDCK-TNPCKNGGIC-IDSINNFECICDNGFFGKTCEERTDYCIKSPCMNYGVCLRHSDELYNDFICECKNNWGGRLCHLP--KHSICDSNPCLNGGSCSRLSTLNGFQCHCNQGWIGTVCQFLHIDFQPCRRKNLCKNEGVCINVGYNDFT---CHCKPGFEGKHC 788
BLAST of slit1 vs. Planmine SMEST
Match: SMESG000027402.1 (SMESG000027402.1) HSP 1 Score: 132.88 bits (333), Expect = 8.619e-31 Identity = 105/355 (29.58%), Postives = 152/355 (42.82%), Query Frame = 1 Query: 2722 ICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDI----------------------NPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENE-----------VISESVCAYHECLNNGIC-----INGDQNKNQKSYCKCKPGYSGHLCQYLNSIYFQKNSYIEISPPSRGFLMPRGNISLNFMTN 3672 +C Y + + C+ PCK+NG C+ + ++Y+C+C F GKNC+ I AC QPC+NGG+C+ +D Y C C G+ G C I C N C+NGA C +N + C C F GK C+ YC + N C G C + C C G+EG CE + DDCK N CK+ C+D N+++C+C G+ GK CE + + S+ Y + E + S+C + CLN G C +NG Q C C G+ G +CQ+L +I+ P R L + +N N Sbjct: 432 LCQPGYTGKYCDKEINECVPNPCKNNGVCT-DSINDYKCNCSTSFKGKNCELNI-ACQNQPCKNGGVCQEVDT--GYTCKCKTGYTGTNCEIYKTGCSPNPCLNGAYCYTINQDFYCHCNKEFWGKRCEHSRPYCGNESCESAYDACSMPQMLNSSGIKHNYCGKYGTCLSHEKEKFICRCMPGFEGDSCEIDIDDCKTNPCKNGGICIDSINNFECICDNGFFGKTCEERTDYCIKSPCMNYGVCLRHSDELYNDFICECKNNWGGRLCHLPKHSICDSNPCLNGGSCSRLSTLNGFQ-------CHCNQGWIGTVCQFL---------HIDFQPCRRKNLCKNEGVCINVGYN 766 HSP 2 Score: 111.694 bits (278), Expect = 2.602e-24 Identity = 70/201 (34.83%), Postives = 106/201 (52.74%), Query Frame = 1 Query: 2767 DSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDN--YGNYECVCPVGFRGPKCTINVDD-CVGNLCVNGATCNDLN--NSYSCKCQSGFRGKYCD---IKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEI 3345 D C PCK+ G C + + +N++C C F GK C++R + C PC N G+C + Y ++ C C + G C + C N C+NG +C+ L+ N + C C G+ G C I F C N C+N G C + ND+ C C G+EGK C+ NT++C N C + +C+D N Y+C+C G+ G C++ Sbjct: 622 DDCKTNPCKNGGIC-IDSINNFECICDNGFFGKTCEERTDYCIKSPCMNYGVCLRHSDELYNDFICECKNNWGGRLCHLPKHSICDSNPCLNGGSCSRLSTLNGFQCHCNQGWIGTVCQFLHIDFQPCRRKNLCKNEGVCINVGYNDFTCHCKPGFEGKHCDINTNECNPNPCFNGGKCIDKLNSYRCICAKGFYGSNCQL 821 HSP 3 Score: 111.694 bits (278), Expect = 3.006e-24 Identity = 101/319 (31.66%), Postives = 135/319 (42.32%), Query Frame = 1 Query: 2764 CDSCMKRP-CKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACF-GQPCRNGGICKNI-DNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQC----------------------------------------QLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEI-------PAWKPTVLKTLISRRDIN-QSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQY 3567 CD CMK P CKH G C + C C + G+ CD+ +N C PC+NGGIC+N+ Y+C+CP GF G C I D C N C G TC + C+C + GK CD C D N CQN GQC Q DYKC+C G+ GK C++ ++C N CK+ C D NDYKC C + GK CE+ P V + + + ++ T N E I ++ C+ + CLN C NQ YC C + G C++ Sbjct: 254 CDECMKYPGCKH-GTC---VDSPWTCSCENNWGGQFCDQSLNYCAENNPCKNGGICENLLSEVQPYKCICPSGFEGRDCEI--DPCFPNPCKKG-TCMKSKGEFYCQCIERWTGKLCDQDTSIC-DQNSCQNGGQCGKDENGNRRCICQQGYKGSECHLAAPCENSQCKHALDCQIQSTALGDYKCLCQPGYTGKYCDKEINECVPNPCKNNGVCTDSINDYKCNCSTSFKGKNCELNIACQNQPCKNGGVCQEVDTGYTCKCKTGYTGTNCE---IYKTGCSPNPCLNGAYCY----TINQDFYCHCNKEFWGKRCEH 557 HSP 4 Score: 110.538 bits (275), Expect = 5.725e-24 Identity = 86/280 (30.71%), Postives = 132/280 (47.14%), Query Frame = 1 Query: 2788 CKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATC----NDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPN-NDYKCVCSKGWEGKQCERNTDDC----KYNSCKHESQCVDL-HNDYKCMCRPGYIGKFCEI--------PAWKP-TVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYL 3570 C G C + C C F G +C+ I+ C PC+NGGIC ID+ N+EC+C GF G C D C+ + C+N C ++L N + C+C++ + G+ C + D NPC N G C L N ++C C++GW G C+ D + N CK+E C+++ +ND+ C C+PG+ GK C+I P + + L S R I N + ++ CA C IC++ + C+C G G LC ++ Sbjct: 590 CGKYGTCLSHEKEKFICRCMPGFEGDSCEIDIDDCKTNPCKNGGIC--IDSINNFECICDNGFFGKTCEERTDYCIKSPCMNYGVCLRHSDELYNDFICECKNNWGGRLCHLPKHSICDSNPCLNGGSCSRLSTLNGFQCHCNQGWIGTVCQFLHIDFQPCRRKNLCKNEGVCINVGYNDFTCHCKPGFEGKHCDINTNECNPNPCFNGGKCIDKLNSYRCICAKGFYGSNCQ---LNYDECASFPCAYGAICVD----RIDDFECRCPEGRDGKLCDHV 860 HSP 5 Score: 104.76 bits (260), Expect = 3.800e-22 Identity = 71/201 (35.32%), Postives = 107/201 (53.23%), Query Frame = 1 Query: 2767 DSCMKRPCKHNGKC---SLITHHNYQCDCPYPFHGKNCD-KRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCT---INVDDCV-GNLCVNGATCNDLN-NSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCE 3342 D C+K PC + G C S ++++ C+C + G+ C + + C PC NGG C + ++C C G+ G C I+ C NLC N C ++ N ++C C+ GF GK+CDI CN NPC N G+C + N Y+C+C+KG+ G C+ N D+C C + + CVD +D++C C G GK C+ Sbjct: 660 DYCIKSPCMNYGVCLRHSDELYNDFICECKNNWGGRLCHLPKHSICDSNPCLNGGSCSRLSTLNGFQCHCNQGWIGTVCQFLHIDFQPCRRKNLCKNEGVCINVGYNDFTCHCKPGFEGKHCDINTNECNP-NPCFNGGKC-IDKLNSYRCICAKGFYGSNCQLNYDECASFPCAYGAICVDRIDDFECRCPEGRDGKLCD 858 HSP 6 Score: 88.5817 bits (218), Expect = 3.563e-17 Identity = 92/358 (25.70%), Postives = 148/358 (41.34%), Query Frame = 1 Query: 2626 FLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKCD---SCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKC-----------------------TINVDDCVGNLCVNGATC-NDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCV----DLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMT----YENLENEVISESVCAY-----------HECLNNGICINGDQNKNQKSYCKCKPGYSGHLC 3561 + ++ D I+ C P K + D+ C+ T ++ C+ +C +PCK+ G C + Y C C + G NC+ C PC NG C I+ ++ C C F G +C +N N C TC + + C+C GF G C+I C NPC+N G C + N+++C+C G+ GK CE TD C + C + C+ +L+ND+ C C+ + G+ C +P K ++ + + S ++ ++ N+ +VC + + C N G+CIN N C CKPG+ G C Sbjct: 437 YTGKYCDKEINECV-PNPCKNNGVCTDSINDYKCNCST--SFKGKNCELNIACQNQPCKNGGVCQEVDT-GYTCKCKTGYTGTNCEIYKTGCSPNPCLNGAYCYTINQ--DFYCHCNKEFWGKRCEHSRPYCGNESCESAYDACSMPQMLNSSGIKHNYCGKYGTCLSHEKEKFICRCMPGFEGDSCEIDIDDCK-TNPCKNGGIC-IDSINNFECICDNGFFGKTCEERTDYCIKSPCMNYGVCLRHSDELYNDFICECKNNWGGRLCHLP--KHSICDSNPCLNGGSCSRLSTLNGFQCHCNQGWIGTVCQFLHIDFQPCRRKNLCKNEGVCINVGYNDFT---CHCKPGFEGKHC 781
BLAST of slit1 vs. Planmine SMEST
Match: SMESG000022173.1 (SMESG000022173.1) HSP 1 Score: 120.168 bits (300), Expect = 6.082e-27 Identity = 87/296 (29.39%), Postives = 127/296 (42.91%), Query Frame = 1 Query: 2800 GKCSL-ITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNS-CKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICI--------NGDQNKNQKSY-----CKCKPGYSGHLCQYLNSIYFQKNSYIEISPP---SRGFL 3633 GKC + + + Y CDCP F+G C +C PC C D ++C CP GF G C I+ D+C C+NG C D + + C C GF G C + C ++ C N +C + N C C+ GW G+ C +N ++C N C + C+D Y+C C+ + G+FC + P + + R+ C + C NNGICI N N+ K CKC PG++G LC+ + S + S P + GFL Sbjct: 330 GKCKINVNTNKYICDCPESFYGDRCQFNQTSCDDNPCVGNSECI-TDKTSGFKCKCPPGFTGRNCEIDEDECSSKPCLNGGMCIDRPSGFLCVCPVGFSGDRCQVNKNDCQKLS-CMNGAKC-MDRANRIDCECTTGWTGRMCNQNINECAINQPCLNNGICIDTPGSYRCQCQATWSGQFCNLKIVVPNI--NYLERK---------------------CLINPCRNNGICILNSLPTSVNIPNNEYFKPIGGPYTCKCNPGFTGDLCE---KVIIANGSIVMYSEPRITTEGFL 596 HSP 2 Score: 90.8929 bits (224), Expect = 6.570e-18 Identity = 82/293 (27.99%), Postives = 121/293 (41.30%), Query Frame = 1 Query: 2740 EDAYITAKCDSCMKRPCKHNGKCSLITHHN----YQCDCPYPFHGKNCDKRINACFGQP--CRNGGICKNIDNYGN---YECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTL--------ISRRDINQSEMTYENLENEVISESVCAYHE-CLNNGICINGDQNKNQKSY-CKCKPGYSGHLC 3561 + + CD C+ P +G C C+C + G C+ +N C P C NGG C N N Y C+C G KC INV+ N Y C C F G C C+D NPC + +C + +KC C G+ G+ CE + D+C C + C+D + + C+C G+ G C++ L + +R D + + N+ I+E CA ++ CLNNGICI+ SY C+C+ +SG C Sbjct: 244 QTGWTGDSCDQCIPYPGCVHGSCRFDETTKELLPLTCNCKKKWGGMFCNIDLNYCSNNPNVCENGGECLNFGNIKTNQQYRCICKPG----KCKINVN----------------TNKYICDCPESFYGDRCQFNQTSCDD-NPCVGNSECITDKTSGFKCKCPPGFTGRNCEIDEDECSSKPCLNGGMCIDRPSGFLCVCPVGFSGDRCQVNKNDCQKLSCMNGAKCMDRANRIDCECTTGWTGRMCNQNINE--CAINQPCLNNGICID-----TPGSYRCQCQATWSGQFC 508 HSP 3 Score: 58.9214 bits (141), Expect = 3.642e-8 Identity = 76/308 (24.68%), Postives = 110/308 (35.71%), Query Frame = 1 Query: 2830 YQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCD---------------------------------------IKFLYC-NDINPCQNSGQC----QLLPNNDYKCVCSKG------------------WEGKQCERNTDDCKYNSCKHESQCV-DLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLENEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQ 3564 ++ C +HG+ CDK +P N N + G+ +C G+ G C V C+ C+NGA C N C+CQ+G+ G CD I YC N+ N C+N G+C + N Y+C+C G + G +C+ N C N C S+C+ D + +KC C PG+ G+ CE I E C+ CLN G+CI ++ C C G+SG CQ Sbjct: 175 FRYTCSQNYHGEECDK-----LCKPSANERGFYNCSSNGDR--ICHTGYSGSYCDKVV--CLKK-CINGA-CVGPN---QCRCQTGWTGDSCDQCIPYPGCVHGSCRFDETTKELLPLTCNCKKKWGGMFCNIDLNYCSNNPNVCENGGECLNFGNIKTNQQYRCICKPGKCKINVNTNKYICDCPESFYGDRCQFNQTSCDDNPCVGNSECITDKTSGFKCKCPPGFTGRNCE--------------------------------IDEDECSSKPCLNGGMCI----DRPSGFLCVCPVGFSGDRCQ 432 The following BLAST results are available for this feature:
BLAST of slit1 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of slit1 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 5
BLAST of slit1 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 5
BLAST of slit1 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of slit1 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of slit1 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of slit1 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of slit1 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of slit1 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of slit1 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 5
BLAST of slit1 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of slit1 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 5
BLAST of slit1 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of slit1 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Cross References
External references for this transcript
Sequences
The following sequences are available for this feature:
transcript sequence >SMED30023930 ID=SMED30023930|Name=slit1|organism=Schmidtea mediterranea sexual|type=transcript|length=4812bpback to top protein sequence of SMED30023930-orf-1 >SMED30023930-orf-1 ID=SMED30023930-orf-1|Name=SMED30023930-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=1535bp MIFIFKDITFKFLLVLLSSLYCFSSTIWINDPSDESEICPNGCQCENYSVback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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