Arginine:serine rich splicing factor

Overview
NameArginine:serine rich splicing factor
Smed IDSMED30023920
Length (bp)976
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Arginine:serine rich splicing factor (SMED30023920) t-SNE clustered cells

Violin plots show distribution of expression levels for Arginine:serine rich splicing factor (SMED30023920) in cells (dots) of each of the 12 neoblast clusters.

 

back to top


 

Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

back to top
Anatomical Expression

PAGE et. al., 2020




SMED30023920

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 4

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
prepharyngeal regionSMED30023920h1SMcG0003904 SmedASXL_021851SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016
whole organism asexual adult RNA-sequencing evidence
parapharyngeal regionSMED30023920h1SMcG0003904 dd_Smed_v6_19158_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017
whole organism asexual adult RNA-sequencing evidence
neoblastSMED30023920h1SMcG0003904 SMED_05855_V2_1GPL14150_gene_modelsPMID:22385657
Wagner et al., 2012
whole organism asexual adult cDNA to DNA expression microarray evidence
neoblastSMED30023920h1SMcG0003904 SMED_05855_V2_1GPL14150PMID:22385657
Wagner et al., 2012
whole organism asexual adult cDNA to DNA expression microarray evidence
Note: Hover over icons to view figure legend
Homology
BLAST of Arginine:serine rich splicing factor vs. Ensembl Human
Match: SRSF1 (serine and arginine rich splicing factor 1 [Source:HGNC Symbol;Acc:HGNC:10780])

HSP 1 Score: 92.4337 bits (228), Expect = 2.178e-22
Identity = 39/81 (48.15%), Postives = 59/81 (72.84%), Query Frame = 3
Query:  483 PPKSTDYRVFISNIPPYSNWQDIKDFMRRYGDVSYADVFGNGTGVCDFVSKEDFRYVLKYVNNVKFKTVSGQTSYIKVEED 725
            P + ++ RV +S +PP  +WQD+KD MR  GDV YADV+ +GTGV +FV KED  Y ++ ++N KF++  G+T+YI+V+ D
Sbjct:   10 PSRRSENRVVVSGLPPSGSWQDLKDHMREAGDVCYADVYRDGTGVVEFVRKEDMTYAVRKLDNTKFRSHEGETAYIRVKVD 90          
BLAST of Arginine:serine rich splicing factor vs. Ensembl Human
Match: SRSF1 (serine and arginine rich splicing factor 1 [Source:HGNC Symbol;Acc:HGNC:10780])

HSP 1 Score: 91.6633 bits (226), Expect = 5.244e-21
Identity = 39/81 (48.15%), Postives = 59/81 (72.84%), Query Frame = 3
Query:  483 PPKSTDYRVFISNIPPYSNWQDIKDFMRRYGDVSYADVFGNGTGVCDFVSKEDFRYVLKYVNNVKFKTVSGQTSYIKVEED 725
            P + ++ RV +S +PP  +WQD+KD MR  GDV YADV+ +GTGV +FV KED  Y ++ ++N KF++  G+T+YI+V+ D
Sbjct:  115 PSRRSENRVVVSGLPPSGSWQDLKDHMREAGDVCYADVYRDGTGVVEFVRKEDMTYAVRKLDNTKFRSHEGETAYIRVKVD 195          

HSP 2 Score: 78.5666 bits (192), Expect = 3.266e-16
Identity = 49/80 (61.25%), Postives = 62/80 (77.50%), Query Frame = 3
Query:   42 PIMSNERKIYVGNLPPDSTSRDIEKLFSCFGKIIDIDIKN-RNGPPFAFIQFXXXXXXXXXXXXXXNYDLDGFSLRVEFP 278
            P  +N+ +IYVGNLPPD  ++DIE +F  +G I DID+KN R GPPFAF++F+D RDAEDA+  RD YD DG+ LRVEFP
Sbjct:   10 PAGNNDCRIYVGNLPPDIRTKDIEDVFYKYGAIRDIDLKNRRGGPPFAFVEFEDPRDAEDAVYGRDGYDYDGYRLRVEFP 89          
BLAST of Arginine:serine rich splicing factor vs. Ensembl Human
Match: SRSF9 (serine and arginine rich splicing factor 9 [Source:HGNC Symbol;Acc:HGNC:10791])

HSP 1 Score: 90.5077 bits (223), Expect = 8.048e-21
Identity = 37/84 (44.05%), Postives = 60/84 (71.43%), Query Frame = 3
Query:  483 PPKSTDYRVFISNIPPYSNWQDIKDFMRRYGDVSYADVFGNGTGVCDFVSKEDFRYVLKYVNNVKFKTVSGQTSYIKVEEDKTT 734
            P + +D+RV +S +PP  +WQD+KD MR  GDV YADV  +G G+ +++ KED  Y L+ +++ KF++  G+TSYI+V  +++T
Sbjct:  105 PTRRSDFRVLVSGLPPSGSWQDLKDHMREAGDVCYADVQKDGVGMVEYLRKEDMEYALRKLDDTKFRSHEGETSYIRVYPERST 188          

HSP 2 Score: 62.003 bits (149), Expect = 1.164e-10
Identity = 42/74 (56.76%), Postives = 55/74 (74.32%), Query Frame = 3
Query:   63 KIYVGNLPPDSTSRDIEKLFSCFGKIIDIDIKNRNG-PPFAFIQFXXXXXXXXXXXXXXNYDLDGFSLRVEFPR 281
            +IYVGNLP D   +D+E LF  +G+I +I++KNR+G  PFAF++F+D RDAEDAI  R+ YD     LRVEFPR
Sbjct:   15 RIYVGNLPTDVREKDLEDLFYKYGRIREIELKNRHGLVPFAFVRFEDPRDAEDAIYGRNGYDYGQCRLRVEFPR 88          
BLAST of Arginine:serine rich splicing factor vs. Ensembl Human
Match: SRSF1 (serine and arginine rich splicing factor 1 [Source:HGNC Symbol;Acc:HGNC:10780])

HSP 1 Score: 91.2781 bits (225), Expect = 8.085e-21
Identity = 39/81 (48.15%), Postives = 59/81 (72.84%), Query Frame = 3
Query:  483 PPKSTDYRVFISNIPPYSNWQDIKDFMRRYGDVSYADVFGNGTGVCDFVSKEDFRYVLKYVNNVKFKTVSGQTSYIKVEED 725
            P + ++ RV +S +PP  +WQD+KD MR  GDV YADV+ +GTGV +FV KED  Y ++ ++N KF++  G+T+YI+V+ D
Sbjct:  115 PSRRSENRVVVSGLPPSGSWQDLKDHMREAGDVCYADVYRDGTGVVEFVRKEDMTYAVRKLDNTKFRSHEGETAYIRVKVD 195          

HSP 2 Score: 78.1814 bits (191), Expect = 3.909e-16
Identity = 49/80 (61.25%), Postives = 62/80 (77.50%), Query Frame = 3
Query:   42 PIMSNERKIYVGNLPPDSTSRDIEKLFSCFGKIIDIDIKN-RNGPPFAFIQFXXXXXXXXXXXXXXNYDLDGFSLRVEFP 278
            P  +N+ +IYVGNLPPD  ++DIE +F  +G I DID+KN R GPPFAF++F+D RDAEDA+  RD YD DG+ LRVEFP
Sbjct:   10 PAGNNDCRIYVGNLPPDIRTKDIEDVFYKYGAIRDIDLKNRRGGPPFAFVEFEDPRDAEDAVYGRDGYDYDGYRLRVEFP 89          
BLAST of Arginine:serine rich splicing factor vs. Ensembl Human
Match: AC015813.2 (novel transcript)

HSP 1 Score: 78.5666 bits (192), Expect = 1.308e-16
Identity = 35/78 (44.87%), Postives = 53/78 (67.95%), Query Frame = 3
Query:  483 PPKSTDYRVFISNIPPYSNWQDIKDFMRRYGDVSYADVFGNGTGVCDFVSKEDFRYVLKYVNNVKFKTVSGQTSYIKV 716
            P + ++ RV +S +PP  +WQD+KD MR  GDV YADV+ +GTGV +FV KED  Y ++ ++N KF+  S +  Y ++
Sbjct:  115 PSRRSENRVVVSGLPPSGSWQDLKDHMREAGDVCYADVYRDGTGVVEFVRKEDMTYAVRKLDNTKFR--SHEVGYTRI 190          

HSP 2 Score: 78.1814 bits (191), Expect = 1.913e-16
Identity = 49/80 (61.25%), Postives = 62/80 (77.50%), Query Frame = 3
Query:   42 PIMSNERKIYVGNLPPDSTSRDIEKLFSCFGKIIDIDIKN-RNGPPFAFIQFXXXXXXXXXXXXXXNYDLDGFSLRVEFP 278
            P  +N+ +IYVGNLPPD  ++DIE +F  +G I DID+KN R GPPFAF++F+D RDAEDA+  RD YD DG+ LRVEFP
Sbjct:   10 PAGNNDCRIYVGNLPPDIRTKDIEDVFYKYGAIRDIDLKNRRGGPPFAFVEFEDPRDAEDAVYGRDGYDYDGYRLRVEFP 89          
BLAST of Arginine:serine rich splicing factor vs. Ensembl Celegans
Match: rsp-3 (Probable splicing factor, arginine/serine-rich 3 [Source:UniProtKB/Swiss-Prot;Acc:Q9NEW6])

HSP 1 Score: 85.8853 bits (211), Expect = 3.218e-19
Identity = 36/75 (48.00%), Postives = 53/75 (70.67%), Query Frame = 3
Query:  501 YRVFISNIPPYSNWQDIKDFMRRYGDVSYADVFGNGTGVCDFVSKEDFRYVLKYVNNVKFKTVSGQTSYIKVEED 725
            YRV +  +PP  +WQD+KD MR  GDV YADV  +GTGV +F   ED +Y ++ +++ KF++  G+T+YI+V ED
Sbjct:  123 YRVIVEGLPPTGSWQDLKDHMRDAGDVCYADVARDGTGVVEFTRYEDVKYAVRKLDDTKFRSHEGETAYIRVRED 197          

HSP 2 Score: 64.3142 bits (155), Expect = 1.169e-11
Identity = 39/75 (52.00%), Postives = 56/75 (74.67%), Query Frame = 3
Query:   51 SNERKIYVGNLPPDSTSRDIEKLFSCFGKIIDIDIKNRNGPPFAFIQFXXXXXXXXXXXXXXNYDLDGFSLRVEF 275
            S ++K+YVGNLP D   +++E +F  +G+I  +DIK+  GP FAF++F+D RDAEDA+R RD Y+ DG  +RVEF
Sbjct:    6 SEDQKVYVGNLPGDVREKEVEDIFHKYGRIKYVDIKSGRGPAFAFVEFEDHRDAEDAVRARDGYEFDGRRIRVEF 80          
BLAST of Arginine:serine rich splicing factor vs. Ensembl Celegans
Match: rsp-3 (Probable splicing factor, arginine/serine-rich 3 [Source:UniProtKB/Swiss-Prot;Acc:Q9NEW6])

HSP 1 Score: 85.8853 bits (211), Expect = 3.218e-19
Identity = 36/75 (48.00%), Postives = 53/75 (70.67%), Query Frame = 3
Query:  501 YRVFISNIPPYSNWQDIKDFMRRYGDVSYADVFGNGTGVCDFVSKEDFRYVLKYVNNVKFKTVSGQTSYIKVEED 725
            YRV +  +PP  +WQD+KD MR  GDV YADV  +GTGV +F   ED +Y ++ +++ KF++  G+T+YI+V ED
Sbjct:  123 YRVIVEGLPPTGSWQDLKDHMRDAGDVCYADVARDGTGVVEFTRYEDVKYAVRKLDDTKFRSHEGETAYIRVRED 197          

HSP 2 Score: 64.3142 bits (155), Expect = 1.169e-11
Identity = 39/75 (52.00%), Postives = 56/75 (74.67%), Query Frame = 3
Query:   51 SNERKIYVGNLPPDSTSRDIEKLFSCFGKIIDIDIKNRNGPPFAFIQFXXXXXXXXXXXXXXNYDLDGFSLRVEF 275
            S ++K+YVGNLP D   +++E +F  +G+I  +DIK+  GP FAF++F+D RDAEDA+R RD Y+ DG  +RVEF
Sbjct:    6 SEDQKVYVGNLPGDVREKEVEDIFHKYGRIKYVDIKSGRGPAFAFVEFEDHRDAEDAVRARDGYEFDGRRIRVEF 80          
BLAST of Arginine:serine rich splicing factor vs. Ensembl Celegans
Match: rsp-3 (Probable splicing factor, arginine/serine-rich 3 [Source:UniProtKB/Swiss-Prot;Acc:Q9NEW6])

HSP 1 Score: 85.8853 bits (211), Expect = 3.218e-19
Identity = 36/75 (48.00%), Postives = 53/75 (70.67%), Query Frame = 3
Query:  501 YRVFISNIPPYSNWQDIKDFMRRYGDVSYADVFGNGTGVCDFVSKEDFRYVLKYVNNVKFKTVSGQTSYIKVEED 725
            YRV +  +PP  +WQD+KD MR  GDV YADV  +GTGV +F   ED +Y ++ +++ KF++  G+T+YI+V ED
Sbjct:  123 YRVIVEGLPPTGSWQDLKDHMRDAGDVCYADVARDGTGVVEFTRYEDVKYAVRKLDDTKFRSHEGETAYIRVRED 197          

HSP 2 Score: 64.3142 bits (155), Expect = 1.169e-11
Identity = 39/75 (52.00%), Postives = 56/75 (74.67%), Query Frame = 3
Query:   51 SNERKIYVGNLPPDSTSRDIEKLFSCFGKIIDIDIKNRNGPPFAFIQFXXXXXXXXXXXXXXNYDLDGFSLRVEF 275
            S ++K+YVGNLP D   +++E +F  +G+I  +DIK+  GP FAF++F+D RDAEDA+R RD Y+ DG  +RVEF
Sbjct:    6 SEDQKVYVGNLPGDVREKEVEDIFHKYGRIKYVDIKSGRGPAFAFVEFEDHRDAEDAVRARDGYEFDGRRIRVEF 80          
BLAST of Arginine:serine rich splicing factor vs. Ensembl Celegans
Match: rsp-3 (Probable splicing factor, arginine/serine-rich 3 [Source:UniProtKB/Swiss-Prot;Acc:Q9NEW6])

HSP 1 Score: 85.8853 bits (211), Expect = 3.218e-19
Identity = 36/75 (48.00%), Postives = 53/75 (70.67%), Query Frame = 3
Query:  501 YRVFISNIPPYSNWQDIKDFMRRYGDVSYADVFGNGTGVCDFVSKEDFRYVLKYVNNVKFKTVSGQTSYIKVEED 725
            YRV +  +PP  +WQD+KD MR  GDV YADV  +GTGV +F   ED +Y ++ +++ KF++  G+T+YI+V ED
Sbjct:  123 YRVIVEGLPPTGSWQDLKDHMRDAGDVCYADVARDGTGVVEFTRYEDVKYAVRKLDDTKFRSHEGETAYIRVRED 197          

HSP 2 Score: 64.3142 bits (155), Expect = 1.169e-11
Identity = 39/75 (52.00%), Postives = 56/75 (74.67%), Query Frame = 3
Query:   51 SNERKIYVGNLPPDSTSRDIEKLFSCFGKIIDIDIKNRNGPPFAFIQFXXXXXXXXXXXXXXNYDLDGFSLRVEF 275
            S ++K+YVGNLP D   +++E +F  +G+I  +DIK+  GP FAF++F+D RDAEDA+R RD Y+ DG  +RVEF
Sbjct:    6 SEDQKVYVGNLPGDVREKEVEDIFHKYGRIKYVDIKSGRGPAFAFVEFEDHRDAEDAVRARDGYEFDGRRIRVEF 80          
BLAST of Arginine:serine rich splicing factor vs. Ensembl Celegans
Match: rsp-3 (Probable splicing factor, arginine/serine-rich 3 [Source:UniProtKB/Swiss-Prot;Acc:Q9NEW6])

HSP 1 Score: 85.8853 bits (211), Expect = 3.218e-19
Identity = 36/75 (48.00%), Postives = 53/75 (70.67%), Query Frame = 3
Query:  501 YRVFISNIPPYSNWQDIKDFMRRYGDVSYADVFGNGTGVCDFVSKEDFRYVLKYVNNVKFKTVSGQTSYIKVEED 725
            YRV +  +PP  +WQD+KD MR  GDV YADV  +GTGV +F   ED +Y ++ +++ KF++  G+T+YI+V ED
Sbjct:  123 YRVIVEGLPPTGSWQDLKDHMRDAGDVCYADVARDGTGVVEFTRYEDVKYAVRKLDDTKFRSHEGETAYIRVRED 197          

HSP 2 Score: 64.3142 bits (155), Expect = 1.169e-11
Identity = 39/75 (52.00%), Postives = 56/75 (74.67%), Query Frame = 3
Query:   51 SNERKIYVGNLPPDSTSRDIEKLFSCFGKIIDIDIKNRNGPPFAFIQFXXXXXXXXXXXXXXNYDLDGFSLRVEF 275
            S ++K+YVGNLP D   +++E +F  +G+I  +DIK+  GP FAF++F+D RDAEDA+R RD Y+ DG  +RVEF
Sbjct:    6 SEDQKVYVGNLPGDVREKEVEDIFHKYGRIKYVDIKSGRGPAFAFVEFEDHRDAEDAVRARDGYEFDGRRIRVEF 80          
BLAST of Arginine:serine rich splicing factor vs. Ensembl Fly
Match: SF2 (gene:FBgn0283477 transcript:FBtr0083272)

HSP 1 Score: 139.428 bits (350), Expect = 3.823e-39
Identity = 84/230 (36.52%), Postives = 122/230 (53.04%), Query Frame = 3
Query:   54 NERKIYVGNLPPDSTSRDIEKLFSCFGKIIDIDIKNRNGPPFAFIQFXXXXXXXXXXXXXXNYDLDGFSLRVEFPR------EIKQNKKDYKEYSRFENPVYVPPFDPYDMYLPYNYGPFPRQDRFXXXXXXXXXXXXXXXXXXXXXPRPPKSTDYRVFISNIPPYSNWQDIKDFMRRYGDVSYADVFGNGTGVCDFVSKEDFRYVLKYVNNVKFKTVSGQTSYIKVEED 725
            NE +IYVGNLPPD  ++DI+ LF  FGK+  +D+KNR GPPFAF++F+D RDA+DA++ RD YD DG+ LRVEFPR          N+ D             PP                                              K + YRV ++ +P   +WQD+KD MR  GDV +AD + +G+GV +F+  ED +Y +K +++ +F++  G+ +YI+V ED
Sbjct:    5 NECRIYVGNLPPDIRTKDIQDLFHKFGKVTFVDLKNRRGPPFAFVEFEDARDADDAVKARDGYDYDGYRLRVEFPRGGGPGSYRGGNRNDRSRDGGGRMGGRGPP---------------------------------------------AKRSQYRVMVTGLPASGSWQDLKDHMREAGDVCFADTYKDGSGVVEFLRHEDMKYAIKKLDDSRFRSHEGEVAYIRVRED 189          
BLAST of Arginine:serine rich splicing factor vs. Ensembl Fly
Match: SF2 (gene:FBgn0283477 transcript:FBtr0304727)

HSP 1 Score: 139.428 bits (350), Expect = 3.823e-39
Identity = 84/230 (36.52%), Postives = 122/230 (53.04%), Query Frame = 3
Query:   54 NERKIYVGNLPPDSTSRDIEKLFSCFGKIIDIDIKNRNGPPFAFIQFXXXXXXXXXXXXXXNYDLDGFSLRVEFPR------EIKQNKKDYKEYSRFENPVYVPPFDPYDMYLPYNYGPFPRQDRFXXXXXXXXXXXXXXXXXXXXXPRPPKSTDYRVFISNIPPYSNWQDIKDFMRRYGDVSYADVFGNGTGVCDFVSKEDFRYVLKYVNNVKFKTVSGQTSYIKVEED 725
            NE +IYVGNLPPD  ++DI+ LF  FGK+  +D+KNR GPPFAF++F+D RDA+DA++ RD YD DG+ LRVEFPR          N+ D             PP                                              K + YRV ++ +P   +WQD+KD MR  GDV +AD + +G+GV +F+  ED +Y +K +++ +F++  G+ +YI+V ED
Sbjct:    5 NECRIYVGNLPPDIRTKDIQDLFHKFGKVTFVDLKNRRGPPFAFVEFEDARDADDAVKARDGYDYDGYRLRVEFPRGGGPGSYRGGNRNDRSRDGGGRMGGRGPP---------------------------------------------AKRSQYRVMVTGLPASGSWQDLKDHMREAGDVCFADTYKDGSGVVEFLRHEDMKYAIKKLDDSRFRSHEGEVAYIRVRED 189          
BLAST of Arginine:serine rich splicing factor vs. Ensembl Fly
Match: B52 (gene:FBgn0004587 transcript:FBtr0082802)

HSP 1 Score: 56.9954 bits (136), Expect = 6.609e-9
Identity = 23/68 (33.82%), Postives = 41/68 (60.29%), Query Frame = 3
Query:  483 PPKSTDYRVFISNIPPYSNWQDIKDFMRRYGDVSYADVFGN--GTGVCDFVSKEDFRYVLKYVNNVKF 680
            PP  T+YR+ + N+    +WQD+KD+MR+ G+V+YAD        GV +F S  D +  ++ +++ + 
Sbjct:  114 PPLRTEYRLIVENLSSRVSWQDLKDYMRQAGEVTYADAHKQRRNEGVVEFASLSDMKTAIEKLDDTEL 181          
BLAST of Arginine:serine rich splicing factor vs. Ensembl Fly
Match: B52 (gene:FBgn0004587 transcript:FBtr0082801)

HSP 1 Score: 56.9954 bits (136), Expect = 6.947e-9
Identity = 23/68 (33.82%), Postives = 41/68 (60.29%), Query Frame = 3
Query:  483 PPKSTDYRVFISNIPPYSNWQDIKDFMRRYGDVSYADVFGN--GTGVCDFVSKEDFRYVLKYVNNVKF 680
            PP  T+YR+ + N+    +WQD+KD+MR+ G+V+YAD        GV +F S  D +  ++ +++ + 
Sbjct:  109 PPLRTEYRLIVENLSSRVSWQDLKDYMRQAGEVTYADAHKQRRNEGVVEFASLSDMKTAIEKLDDTEL 176          
BLAST of Arginine:serine rich splicing factor vs. Ensembl Fly
Match: B52 (gene:FBgn0004587 transcript:FBtr0308196)

HSP 1 Score: 56.9954 bits (136), Expect = 6.947e-9
Identity = 23/68 (33.82%), Postives = 41/68 (60.29%), Query Frame = 3
Query:  483 PPKSTDYRVFISNIPPYSNWQDIKDFMRRYGDVSYADVFGN--GTGVCDFVSKEDFRYVLKYVNNVKF 680
            PP  T+YR+ + N+    +WQD+KD+MR+ G+V+YAD        GV +F S  D +  ++ +++ + 
Sbjct:  109 PPLRTEYRLIVENLSSRVSWQDLKDYMRQAGEVTYADAHKQRRNEGVVEFASLSDMKTAIEKLDDTEL 176          
BLAST of Arginine:serine rich splicing factor vs. Ensembl Zebrafish
Match: srsf1a (serine and arginine rich splicing factor 1a [Source:NCBI gene;Acc:406288])

HSP 1 Score: 95.9005 bits (237), Expect = 9.448e-23
Identity = 41/81 (50.62%), Postives = 60/81 (74.07%), Query Frame = 3
Query:  483 PPKSTDYRVFISNIPPYSNWQDIKDFMRRYGDVSYADVFGNGTGVCDFVSKEDFRYVLKYVNNVKFKTVSGQTSYIKVEED 725
            P + ++YRV +S +PP  +WQD+KD MR  GDV YADVF +GTGV +FV KED  Y ++ ++N KF++  G+T+YI+V+ D
Sbjct:  111 PSRRSEYRVIVSGLPPSGSWQDLKDHMREAGDVCYADVFRDGTGVVEFVRKEDMTYAVRKLDNTKFRSHEGETAYIRVKVD 191          

HSP 2 Score: 79.337 bits (194), Expect = 8.037e-17
Identity = 49/80 (61.25%), Postives = 62/80 (77.50%), Query Frame = 3
Query:   42 PIMSNERKIYVGNLPPDSTSRDIEKLFSCFGKIIDIDIKN-RNGPPFAFIQFXXXXXXXXXXXXXXNYDLDGFSLRVEFP 278
            P  SN+ +IYVGNLPPD  ++D+E +F  +G I DID+KN R GPPFAF++F+D RDAEDA+  RD YD DG+ LRVEFP
Sbjct:    9 PAGSNDCRIYVGNLPPDIRTKDVEDVFYKYGAIRDIDLKNRRGGPPFAFVEFEDPRDAEDAVYARDGYDYDGYRLRVEFP 88          
BLAST of Arginine:serine rich splicing factor vs. Ensembl Zebrafish
Match: srsf1a (serine and arginine rich splicing factor 1a [Source:NCBI gene;Acc:406288])

HSP 1 Score: 95.9005 bits (237), Expect = 9.736e-23
Identity = 41/81 (50.62%), Postives = 60/81 (74.07%), Query Frame = 3
Query:  483 PPKSTDYRVFISNIPPYSNWQDIKDFMRRYGDVSYADVFGNGTGVCDFVSKEDFRYVLKYVNNVKFKTVSGQTSYIKVEED 725
            P + ++YRV +S +PP  +WQD+KD MR  GDV YADVF +GTGV +FV KED  Y ++ ++N KF++  G+T+YI+V+ D
Sbjct:  124 PSRRSEYRVIVSGLPPSGSWQDLKDHMREAGDVCYADVFRDGTGVVEFVRKEDMTYAVRKLDNTKFRSHEGETAYIRVKVD 204          

HSP 2 Score: 81.6481 bits (200), Expect = 1.837e-17
Identity = 50/81 (61.73%), Postives = 63/81 (77.78%), Query Frame = 3
Query:   42 PIMSNERKIYVGNLPPDSTSRDIEKLFSCFGKIIDIDIKN-RNGPPFAFIQFXXXXXXXXXXXXXXNYDLDGFSLRVEFPR 281
            P  SN+ +IYVGNLPPD  ++D+E +F  +G I DID+KN R GPPFAF++F+D RDAEDA+  RD YD DG+ LRVEFPR
Sbjct:    9 PAGSNDCRIYVGNLPPDIRTKDVEDVFYKYGAIRDIDLKNRRGGPPFAFVEFEDPRDAEDAVYARDGYDYDGYRLRVEFPR 89          
BLAST of Arginine:serine rich splicing factor vs. Ensembl Zebrafish
Match: srsf1a (serine and arginine rich splicing factor 1a [Source:NCBI gene;Acc:406288])

HSP 1 Score: 95.9005 bits (237), Expect = 9.736e-23
Identity = 41/81 (50.62%), Postives = 60/81 (74.07%), Query Frame = 3
Query:  483 PPKSTDYRVFISNIPPYSNWQDIKDFMRRYGDVSYADVFGNGTGVCDFVSKEDFRYVLKYVNNVKFKTVSGQTSYIKVEED 725
            P + ++YRV +S +PP  +WQD+KD MR  GDV YADVF +GTGV +FV KED  Y ++ ++N KF++  G+T+YI+V+ D
Sbjct:  124 PSRRSEYRVIVSGLPPSGSWQDLKDHMREAGDVCYADVFRDGTGVVEFVRKEDMTYAVRKLDNTKFRSHEGETAYIRVKVD 204          

HSP 2 Score: 81.6481 bits (200), Expect = 1.837e-17
Identity = 50/81 (61.73%), Postives = 63/81 (77.78%), Query Frame = 3
Query:   42 PIMSNERKIYVGNLPPDSTSRDIEKLFSCFGKIIDIDIKN-RNGPPFAFIQFXXXXXXXXXXXXXXNYDLDGFSLRVEFPR 281
            P  SN+ +IYVGNLPPD  ++D+E +F  +G I DID+KN R GPPFAF++F+D RDAEDA+  RD YD DG+ LRVEFPR
Sbjct:    9 PAGSNDCRIYVGNLPPDIRTKDVEDVFYKYGAIRDIDLKNRRGGPPFAFVEFEDPRDAEDAVYARDGYDYDGYRLRVEFPR 89          
BLAST of Arginine:serine rich splicing factor vs. Ensembl Zebrafish
Match: srsf1b (serine and arginine rich splicing factor 1b [Source:NCBI gene;Acc:393565])

HSP 1 Score: 95.5153 bits (236), Expect = 1.112e-22
Identity = 41/81 (50.62%), Postives = 60/81 (74.07%), Query Frame = 3
Query:  483 PPKSTDYRVFISNIPPYSNWQDIKDFMRRYGDVSYADVFGNGTGVCDFVSKEDFRYVLKYVNNVKFKTVSGQTSYIKVEED 725
            P + ++YRV +S +PP  +WQD+KD MR  GDV YADVF +GTGV +FV KED  Y ++ ++N KF++  G+T+YI+V+ D
Sbjct:  114 PSRRSEYRVIVSGLPPSGSWQDLKDHMREAGDVCYADVFRDGTGVVEFVRKEDMTYAVRKLDNTKFRSHEGETAYIRVKVD 194          

HSP 2 Score: 77.411 bits (189), Expect = 3.698e-16
Identity = 48/80 (60.00%), Postives = 62/80 (77.50%), Query Frame = 3
Query:   42 PIMSNERKIYVGNLPPDSTSRDIEKLFSCFGKIIDIDIKN-RNGPPFAFIQFXXXXXXXXXXXXXXNYDLDGFSLRVEFP 278
            P  +N+ +IYVGNLPPD  ++D+E +F  +G I DID+KN R GPPFAF++F+D RDAEDA+  RD YD DG+ LRVEFP
Sbjct:    9 PAGNNDCRIYVGNLPPDIRTKDVEDVFYKYGAIRDIDLKNRRGGPPFAFVEFEDPRDAEDAVYGRDGYDYDGYRLRVEFP 88          
BLAST of Arginine:serine rich splicing factor vs. Ensembl Zebrafish
Match: srsf9 (serine and arginine rich splicing factor 9 [Source:ZFIN;Acc:ZDB-GENE-040426-2397])

HSP 1 Score: 86.6557 bits (213), Expect = 2.108e-19
Identity = 33/82 (40.24%), Postives = 59/82 (71.95%), Query Frame = 3
Query:  489 KSTDYRVFISNIPPYSNWQDIKDFMRRYGDVSYADVFGNGTGVCDFVSKEDFRYVLKYVNNVKFKTVSGQTSYIKVEEDKTT 734
            + +++RV ++ +PP  +WQD+KD MR  GDV +ADV  +G GV +F+ +ED  Y L+ +++ +F++  G+T+YI+V E++ T
Sbjct:  105 RRSEFRVIVTGLPPTGSWQDLKDHMREAGDVCFADVQRDGEGVVEFLRREDMEYALRRLDSTEFRSHQGETAYIRVMEERGT 186          

HSP 2 Score: 60.077 bits (144), Expect = 4.710e-10
Identity = 45/79 (56.96%), Postives = 56/79 (70.89%), Query Frame = 3
Query:   48 MSNERKIYVGNLPPDSTSRDIEKLFSCFGKIIDIDIK-NRNGPPFAFIQFXXXXXXXXXXXXXXNYDLDGFSLRVEFPR 281
            MS+ R IYVGNLP D   RDIE LF  +GKI DI++K NR+  PFAF++F+D RDAEDA+  R+ Y      LRVE+PR
Sbjct:    1 MSDGR-IYVGNLPMDVQERDIEDLFFKYGKIRDIELKNNRSTIPFAFVRFEDPRDAEDAVFGRNGYGFGDCKLRVEYPR 78          
BLAST of Arginine:serine rich splicing factor vs. Ensembl Xenopus
Match: tent5a (terminal nucleotidyltransferase 5A [Source:Xenbase;Acc:XB-GENE-5843475])

HSP 1 Score: 96.2857 bits (238), Expect = 1.495e-22
Identity = 41/81 (50.62%), Postives = 60/81 (74.07%), Query Frame = 3
Query:  483 PPKSTDYRVFISNIPPYSNWQDIKDFMRRYGDVSYADVFGNGTGVCDFVSKEDFRYVLKYVNNVKFKTVSGQTSYIKVEED 725
            P + ++YRV +S +PP  +WQD+KD MR  GDV YADVF +GTGV +FV KED  Y ++ ++N KF++  G+T+YI+V+ D
Sbjct:  134 PSRRSEYRVVVSGLPPSGSWQDLKDHMREAGDVCYADVFRDGTGVVEFVRKEDMTYAVRKLDNTKFRSHEGETAYIRVKVD 214          

HSP 2 Score: 80.4925 bits (197), Expect = 7.706e-17
Identity = 50/81 (61.73%), Postives = 63/81 (77.78%), Query Frame = 3
Query:   42 PIMSNERKIYVGNLPPDSTSRDIEKLFSCFGKIIDIDIKN-RNGPPFAFIQFXXXXXXXXXXXXXXNYDLDGFSLRVEFPR 281
            P  +N+ +IYVGNLPPD  ++DIE +F  +G I DID+KN R GPPFAF++F+D RDAEDA+  RD YD DG+ LRVEFPR
Sbjct:   10 PAGNNDCRIYVGNLPPDIRTKDIEDVFYKYGAIRDIDLKNRRGGPPFAFVEFEDPRDAEDAVYGRDGYDYDGYRLRVEFPR 90          
BLAST of Arginine:serine rich splicing factor vs. Ensembl Xenopus
Match: tent5a (terminal nucleotidyltransferase 5A [Source:Xenbase;Acc:XB-GENE-5843475])

HSP 1 Score: 95.5153 bits (236), Expect = 2.434e-22
Identity = 41/81 (50.62%), Postives = 60/81 (74.07%), Query Frame = 3
Query:  483 PPKSTDYRVFISNIPPYSNWQDIKDFMRRYGDVSYADVFGNGTGVCDFVSKEDFRYVLKYVNNVKFKTVSGQTSYIKVEED 725
            P + ++YRV +S +PP  +WQD+KD MR  GDV YADVF +GTGV +FV KED  Y ++ ++N KF++  G+T+YI+V+ D
Sbjct:  134 PSRRSEYRVVVSGLPPSGSWQDLKDHMREAGDVCYADVFRDGTGVVEFVRKEDMTYAVRKLDNTKFRSHEGETAYIRVKVD 214          

HSP 2 Score: 80.1073 bits (196), Expect = 9.547e-17
Identity = 50/81 (61.73%), Postives = 63/81 (77.78%), Query Frame = 3
Query:   42 PIMSNERKIYVGNLPPDSTSRDIEKLFSCFGKIIDIDIKN-RNGPPFAFIQFXXXXXXXXXXXXXXNYDLDGFSLRVEFPR 281
            P  +N+ +IYVGNLPPD  ++DIE +F  +G I DID+KN R GPPFAF++F+D RDAEDA+  RD YD DG+ LRVEFPR
Sbjct:   10 PAGNNDCRIYVGNLPPDIRTKDIEDVFYKYGAIRDIDLKNRRGGPPFAFVEFEDPRDAEDAVYGRDGYDYDGYRLRVEFPR 90          
BLAST of Arginine:serine rich splicing factor vs. Ensembl Xenopus
Match: lhx3 (LIM homeobox 3 [Source:Xenbase;Acc:XB-GENE-490075])

HSP 1 Score: 92.4337 bits (228), Expect = 1.587e-21
Identity = 37/78 (47.44%), Postives = 56/78 (71.79%), Query Frame = 3
Query:  483 PPKSTDYRVFISNIPPYSNWQDIKDFMRRYGDVSYADVFGNGTGVCDFVSKEDFRYVLKYVNNVKFKTVSGQTSYIKV 716
            P + ++YRV +S +PP  +WQD+KD MR  GDV YADV  +G G+ +F+ KED  Y L+ +++ KF++  G+TSYI+V
Sbjct:  111 PSRRSEYRVIVSGLPPSGSWQDLKDHMREAGDVCYADVHKDGMGIVEFIRKEDMEYALRKLDDTKFRSHEGETSYIRV 188          

HSP 2 Score: 61.2326 bits (147), Expect = 2.252e-10
Identity = 41/80 (51.25%), Postives = 53/80 (66.25%), Query Frame = 3
Query:   51 SNERKIYVGNLPPDSTSRDIEKLFSCFGKIIDIDIKNRNG---PPFAFIQFXXXXXXXXXXXXXXNYDLDGFSLRVEFPR 281
            S + +IYVGNLP D   +++E LF  +G+I  I++KNR G    PFAFI F D RDAEDA+  R+ Y+     LRVEFPR
Sbjct:   13 SGDGRIYVGNLPADIREKELEDLFDRYGRIRTIELKNRGGSSAAPFAFISFQDPRDAEDAVFARNGYEFGSCRLRVEFPR 92          
BLAST of Arginine:serine rich splicing factor vs. Ensembl Xenopus
Match: grm6 (glutamate receptor, metabotropic 6 [Source:Xenbase;Acc:XB-GENE-949374])

HSP 1 Score: 73.1738 bits (178), Expect = 1.192e-13
Identity = 61/204 (29.90%), Postives = 93/204 (45.59%), Query Frame = 3
Query:   63 KIYVGNLPPDSTSRDIEKLFSCFGKIIDIDIKNRNGPPFAFIQFXXXXXXXXXXXXXXNYDLDGFSLRVEFPREIKQNKKDYKEYSRFENPVYVPPFDPYDMYLPYNYGPFPRQDRFXXXXXXXXXXXXXXXXXXXXXPRPPKSTDYRVFISNIPPYSNWQDIKDFMRRYGDVSYADVFG--NGTGVCDFVSKEDFRYVLKYVN 668
            ++Y+G L   +  RD+E+ F  FGKI+++D+KN     + F++F+D RDAEDA+ E +  +L G  + VE  R                     P  D    Y  Y  G     D++                       PP  T YR+ + N+    +WQD+KDFMR+ G+V+YAD        GV +F S  D R  L+ ++
Sbjct:    8 RVYIGRLSHRARERDVERFFKGFGKIVEVDLKN----GYGFVEFEDSRDAEDAVYEMNGRELCGERVIVEHAR--------------------APRRDIRSGY-GYRKGGS---DKYG----------------------PPVRTMYRLRVENLSSRCSWQDLKDFMRQAGEVTYADAHQRRQNEGVIEFRSYSDMRRALEKLD 161          
BLAST of Arginine:serine rich splicing factor vs. Ensembl Xenopus
Match: grm6 (glutamate receptor, metabotropic 6 [Source:Xenbase;Acc:XB-GENE-949374])

HSP 1 Score: 73.1738 bits (178), Expect = 1.201e-13
Identity = 61/204 (29.90%), Postives = 93/204 (45.59%), Query Frame = 3
Query:   63 KIYVGNLPPDSTSRDIEKLFSCFGKIIDIDIKNRNGPPFAFIQFXXXXXXXXXXXXXXNYDLDGFSLRVEFPREIKQNKKDYKEYSRFENPVYVPPFDPYDMYLPYNYGPFPRQDRFXXXXXXXXXXXXXXXXXXXXXPRPPKSTDYRVFISNIPPYSNWQDIKDFMRRYGDVSYADVFG--NGTGVCDFVSKEDFRYVLKYVN 668
            ++Y+G L   +  RD+E+ F  FGKI+++D+KN     + F++F+D RDAEDA+ E +  +L G  + VE  R                     P  D    Y  Y  G     D++                       PP  T YR+ + N+    +WQD+KDFMR+ G+V+YAD        GV +F S  D R  L+ ++
Sbjct:    8 RVYIGRLSHRARERDVERFFKGFGKIVEVDLKN----GYGFVEFEDSRDAEDAVYEMNGRELCGERVIVEHAR--------------------APRRDIRSGY-GYRKGGS---DKYG----------------------PPVRTMYRLRVENLSSRCSWQDLKDFMRQAGEVTYADAHQRRQNEGVIEFRSYSDMRRALEKLD 161          
BLAST of Arginine:serine rich splicing factor vs. Ensembl Mouse
Match: Srsf9 (serine/arginine-rich splicing factor 9 [Source:MGI Symbol;Acc:MGI:104896])

HSP 1 Score: 123.25 bits (308), Expect = 3.061e-33
Identity = 83/225 (36.89%), Postives = 120/225 (53.33%), Query Frame = 3
Query:   63 KIYVGNLPPDSTSRDIEKLFSCFGKIIDIDIKNRNG-PPFAFIQFXXXXXXXXXXXXXXNYDLDGFSLRVEFPREIKQNKKDYKEYSRFENPVYVPPFDPYDMYLPYNYGPFPRQDRFXXXXXXXXXXXXXXXXXXXXXPRPPKSTDYRVFISNIPPYSNWQDIKDFMRRYGDVSYADVFGNGTGVCDFVSKEDFRYVLKYVNNVKFKTVSGQTSYIKVEEDKTT 734
            +IYVGNLP D   +D+E LF  +G+I +I++KNR+G  PFAF++F+D RDAEDAI  R+ YD     LRVEFPR                                   G +PR  R                        P + +D+RV +S +PP  +WQD+KD MR  GDV YADV  +G G+ +++ KED  Y L+ +++ KF++  G+TSYI+V  +++T
Sbjct:   16 RIYVGNLPSDVREKDLEDLFYKYGRIREIELKNRHGLVPFAFVRFEDPRDAEDAIYGRNGYDYGQCRLRVEFPRTYGGR------------------------------GGWPRGARNGP---------------------PTRRSDFRVLVSGLPPSGSWQDLKDHMREAGDVCYADVQKDGMGMVEYLRKEDMEYALRKLDDTKFRSHEGETSYIRVYPERST 189          
BLAST of Arginine:serine rich splicing factor vs. Ensembl Mouse
Match: Srsf1 (serine/arginine-rich splicing factor 1 [Source:MGI Symbol;Acc:MGI:98283])

HSP 1 Score: 91.6633 bits (226), Expect = 3.600e-21
Identity = 39/81 (48.15%), Postives = 59/81 (72.84%), Query Frame = 3
Query:  483 PPKSTDYRVFISNIPPYSNWQDIKDFMRRYGDVSYADVFGNGTGVCDFVSKEDFRYVLKYVNNVKFKTVSGQTSYIKVEED 725
            P + ++ RV +S +PP  +WQD+KD MR  GDV YADV+ +GTGV +FV KED  Y ++ ++N KF++  G+T+YI+V+ D
Sbjct:  115 PSRRSENRVVVSGLPPSGSWQDLKDHMREAGDVCYADVYRDGTGVVEFVRKEDMTYAVRKLDNTKFRSHEGETAYIRVKVD 195          

HSP 2 Score: 78.5666 bits (192), Expect = 2.243e-16
Identity = 49/80 (61.25%), Postives = 62/80 (77.50%), Query Frame = 3
Query:   42 PIMSNERKIYVGNLPPDSTSRDIEKLFSCFGKIIDIDIKN-RNGPPFAFIQFXXXXXXXXXXXXXXNYDLDGFSLRVEFP 278
            P  +N+ +IYVGNLPPD  ++DIE +F  +G I DID+KN R GPPFAF++F+D RDAEDA+  RD YD DG+ LRVEFP
Sbjct:   10 PAGNNDCRIYVGNLPPDIRTKDIEDVFYKYGAIRDIDLKNRRGGPPFAFVEFEDPRDAEDAVYGRDGYDYDGYRLRVEFP 89          
BLAST of Arginine:serine rich splicing factor vs. Ensembl Mouse
Match: Srsf1 (serine/arginine-rich splicing factor 1 [Source:MGI Symbol;Acc:MGI:98283])

HSP 1 Score: 91.6633 bits (226), Expect = 3.600e-21
Identity = 39/81 (48.15%), Postives = 59/81 (72.84%), Query Frame = 3
Query:  483 PPKSTDYRVFISNIPPYSNWQDIKDFMRRYGDVSYADVFGNGTGVCDFVSKEDFRYVLKYVNNVKFKTVSGQTSYIKVEED 725
            P + ++ RV +S +PP  +WQD+KD MR  GDV YADV+ +GTGV +FV KED  Y ++ ++N KF++  G+T+YI+V+ D
Sbjct:  115 PSRRSENRVVVSGLPPSGSWQDLKDHMREAGDVCYADVYRDGTGVVEFVRKEDMTYAVRKLDNTKFRSHEGETAYIRVKVD 195          

HSP 2 Score: 78.5666 bits (192), Expect = 2.243e-16
Identity = 49/80 (61.25%), Postives = 62/80 (77.50%), Query Frame = 3
Query:   42 PIMSNERKIYVGNLPPDSTSRDIEKLFSCFGKIIDIDIKN-RNGPPFAFIQFXXXXXXXXXXXXXXNYDLDGFSLRVEFP 278
            P  +N+ +IYVGNLPPD  ++DIE +F  +G I DID+KN R GPPFAF++F+D RDAEDA+  RD YD DG+ LRVEFP
Sbjct:   10 PAGNNDCRIYVGNLPPDIRTKDIEDVFYKYGAIRDIDLKNRRGGPPFAFVEFEDPRDAEDAVYGRDGYDYDGYRLRVEFP 89          
BLAST of Arginine:serine rich splicing factor vs. Ensembl Mouse
Match: Srsf1 (serine/arginine-rich splicing factor 1 [Source:MGI Symbol;Acc:MGI:98283])

HSP 1 Score: 91.2781 bits (225), Expect = 5.550e-21
Identity = 39/81 (48.15%), Postives = 59/81 (72.84%), Query Frame = 3
Query:  483 PPKSTDYRVFISNIPPYSNWQDIKDFMRRYGDVSYADVFGNGTGVCDFVSKEDFRYVLKYVNNVKFKTVSGQTSYIKVEED 725
            P + ++ RV +S +PP  +WQD+KD MR  GDV YADV+ +GTGV +FV KED  Y ++ ++N KF++  G+T+YI+V+ D
Sbjct:  115 PSRRSENRVVVSGLPPSGSWQDLKDHMREAGDVCYADVYRDGTGVVEFVRKEDMTYAVRKLDNTKFRSHEGETAYIRVKVD 195          

HSP 2 Score: 78.1814 bits (191), Expect = 2.684e-16
Identity = 49/80 (61.25%), Postives = 62/80 (77.50%), Query Frame = 3
Query:   42 PIMSNERKIYVGNLPPDSTSRDIEKLFSCFGKIIDIDIKN-RNGPPFAFIQFXXXXXXXXXXXXXXNYDLDGFSLRVEFP 278
            P  +N+ +IYVGNLPPD  ++DIE +F  +G I DID+KN R GPPFAF++F+D RDAEDA+  RD YD DG+ LRVEFP
Sbjct:   10 PAGNNDCRIYVGNLPPDIRTKDIEDVFYKYGAIRDIDLKNRRGGPPFAFVEFEDPRDAEDAVYGRDGYDYDGYRLRVEFP 89          
BLAST of Arginine:serine rich splicing factor vs. Ensembl Mouse
Match: Srsf1 (serine/arginine-rich splicing factor 1 [Source:MGI Symbol;Acc:MGI:98283])

HSP 1 Score: 91.2781 bits (225), Expect = 5.550e-21
Identity = 39/81 (48.15%), Postives = 59/81 (72.84%), Query Frame = 3
Query:  483 PPKSTDYRVFISNIPPYSNWQDIKDFMRRYGDVSYADVFGNGTGVCDFVSKEDFRYVLKYVNNVKFKTVSGQTSYIKVEED 725
            P + ++ RV +S +PP  +WQD+KD MR  GDV YADV+ +GTGV +FV KED  Y ++ ++N KF++  G+T+YI+V+ D
Sbjct:  115 PSRRSENRVVVSGLPPSGSWQDLKDHMREAGDVCYADVYRDGTGVVEFVRKEDMTYAVRKLDNTKFRSHEGETAYIRVKVD 195          

HSP 2 Score: 78.1814 bits (191), Expect = 2.684e-16
Identity = 49/80 (61.25%), Postives = 62/80 (77.50%), Query Frame = 3
Query:   42 PIMSNERKIYVGNLPPDSTSRDIEKLFSCFGKIIDIDIKN-RNGPPFAFIQFXXXXXXXXXXXXXXNYDLDGFSLRVEFP 278
            P  +N+ +IYVGNLPPD  ++DIE +F  +G I DID+KN R GPPFAF++F+D RDAEDA+  RD YD DG+ LRVEFP
Sbjct:   10 PAGNNDCRIYVGNLPPDIRTKDIEDVFYKYGAIRDIDLKNRRGGPPFAFVEFEDPRDAEDAVYGRDGYDYDGYRLRVEFP 89          
BLAST of Arginine:serine rich splicing factor vs. UniProt/SwissProt
Match: sp|Q5PPI1|SRSF9_RAT (Serine/arginine-rich splicing factor 9 OS=Rattus norvegicus OX=10116 GN=Srsf9 PE=1 SV=1)

HSP 1 Score: 124.02 bits (310), Expect = 1.206e-32
Identity = 84/228 (36.84%), Postives = 120/228 (52.63%), Query Frame = 3
Query:   63 KIYVGNLPPDSTSRDIEKLFSCFGKIIDIDIKNRNG-PPFAFIQFXXXXXXXXXXXXXXNYDLDGFSLRVEFPREIKQNKKDYKEYSRFENPVYVPPFDPYDMYLPYNYGPFPRQDRFXXXXXXXXXXXXXXXXXXXXXPRPPKSTDYRVFISNIPPYSNWQDIKDFMRRYGDVSYADVFGNGTGVCDFVSKEDFRYVLKYVNNVKFKTVSGQTSYIKVEEDKTTKRG 743
            +IYVGNLP D   +D+E LF  +G+I +I++KNR+G  PFAF++F+D RDAEDAI  R+ YD     LRVEFPR                                   G +PR  R                        P + +D+RV +S +PP  +WQD+KD MR  GDV YADV  +G G+ +++ KED  Y L+ +++ KF++  G+TSYI+V  ++ T  G
Sbjct:   15 RIYVGNLPTDVREKDLEDLFYKYGRIREIELKNRHGLVPFAFVRFEDPRDAEDAIYGRNGYDYGQCRLRVEFPRAYGGR------------------------------GGWPRASRNGP---------------------PTRRSDFRVLVSGLPPSGSWQDLKDHMREAGDVCYADVQKDGMGMVEYLRKEDMEYALRKLDDTKFRSHEGETSYIRVYPERGTSYG 191          
BLAST of Arginine:serine rich splicing factor vs. UniProt/SwissProt
Match: sp|Q9D0B0|SRSF9_MOUSE (Serine/arginine-rich splicing factor 9 OS=Mus musculus OX=10090 GN=Srsf9 PE=1 SV=1)

HSP 1 Score: 123.25 bits (308), Expect = 2.141e-32
Identity = 83/225 (36.89%), Postives = 120/225 (53.33%), Query Frame = 3
Query:   63 KIYVGNLPPDSTSRDIEKLFSCFGKIIDIDIKNRNG-PPFAFIQFXXXXXXXXXXXXXXNYDLDGFSLRVEFPREIKQNKKDYKEYSRFENPVYVPPFDPYDMYLPYNYGPFPRQDRFXXXXXXXXXXXXXXXXXXXXXPRPPKSTDYRVFISNIPPYSNWQDIKDFMRRYGDVSYADVFGNGTGVCDFVSKEDFRYVLKYVNNVKFKTVSGQTSYIKVEEDKTT 734
            +IYVGNLP D   +D+E LF  +G+I +I++KNR+G  PFAF++F+D RDAEDAI  R+ YD     LRVEFPR                                   G +PR  R                        P + +D+RV +S +PP  +WQD+KD MR  GDV YADV  +G G+ +++ KED  Y L+ +++ KF++  G+TSYI+V  +++T
Sbjct:   16 RIYVGNLPSDVREKDLEDLFYKYGRIREIELKNRHGLVPFAFVRFEDPRDAEDAIYGRNGYDYGQCRLRVEFPRTYGGR------------------------------GGWPRGARNGP---------------------PTRRSDFRVLVSGLPPSGSWQDLKDHMREAGDVCYADVQKDGMGMVEYLRKEDMEYALRKLDDTKFRSHEGETSYIRVYPERST 189          
BLAST of Arginine:serine rich splicing factor vs. UniProt/SwissProt
Match: sp|Q5ZML3|SRSF1_CHICK (Serine/arginine-rich splicing factor 1 OS=Gallus gallus OX=9031 GN=SRSF1 PE=1 SV=3)

HSP 1 Score: 98.9821 bits (245), Expect = 6.436e-23
Identity = 44/95 (46.32%), Postives = 64/95 (67.37%), Query Frame = 3
Query:  483 PPKSTDYRVFISNIPPYSNWQDIKDFMRRYGDVSYADVFGNGTGVCDFVSKEDFRYVLKYVNNVKFKTVSGQTSYIKVEEDKTTKRGYHRRSPRT 767
            P + ++YRV +S +PP  +WQD+KD MR  GDV YADVF +GTGV +FV KED  Y ++ ++N KF++  G+T+YI+V+ D      Y R   R+
Sbjct:  115 PSRRSEYRVIVSGLPPSGSWQDLKDHMREAGDVCYADVFRDGTGVVEFVRKEDMTYAVRKLDNTKFRSHEGETAYIRVKVDGPRSPSYGRSRSRS 209          

HSP 2 Score: 78.1814 bits (191), Expect = 1.968e-15
Identity = 49/80 (61.25%), Postives = 62/80 (77.50%), Query Frame = 3
Query:   42 PIMSNERKIYVGNLPPDSTSRDIEKLFSCFGKIIDIDIKN-RNGPPFAFIQFXXXXXXXXXXXXXXNYDLDGFSLRVEFP 278
            P  +N+ +IYVGNLPPD  ++DIE +F  +G I DID+KN R GPPFAF++F+D RDAEDA+  RD YD DG+ LRVEFP
Sbjct:   10 PAGNNDCRIYVGNLPPDIRTKDIEDVFYKYGAIRDIDLKNRRGGPPFAFVEFEDPRDAEDAVYGRDGYDYDGYRLRVEFP 89          
BLAST of Arginine:serine rich splicing factor vs. UniProt/SwissProt
Match: sp|Q7SXP4|SRS1A_DANRE (Serine/arginine-rich splicing factor 1A OS=Danio rerio OX=7955 GN=srsf1a PE=2 SV=2)

HSP 1 Score: 95.9005 bits (237), Expect = 7.350e-22
Identity = 41/81 (50.62%), Postives = 60/81 (74.07%), Query Frame = 3
Query:  483 PPKSTDYRVFISNIPPYSNWQDIKDFMRRYGDVSYADVFGNGTGVCDFVSKEDFRYVLKYVNNVKFKTVSGQTSYIKVEED 725
            P + ++YRV +S +PP  +WQD+KD MR  GDV YADVF +GTGV +FV KED  Y ++ ++N KF++  G+T+YI+V+ D
Sbjct:  124 PSRRSEYRVIVSGLPPSGSWQDLKDHMREAGDVCYADVFRDGTGVVEFVRKEDMTYAVRKLDNTKFRSHEGETAYIRVKVD 204          

HSP 2 Score: 81.6481 bits (200), Expect = 1.387e-16
Identity = 50/81 (61.73%), Postives = 63/81 (77.78%), Query Frame = 3
Query:   42 PIMSNERKIYVGNLPPDSTSRDIEKLFSCFGKIIDIDIKN-RNGPPFAFIQFXXXXXXXXXXXXXXNYDLDGFSLRVEFPR 281
            P  SN+ +IYVGNLPPD  ++D+E +F  +G I DID+KN R GPPFAF++F+D RDAEDA+  RD YD DG+ LRVEFPR
Sbjct:    9 PAGSNDCRIYVGNLPPDIRTKDVEDVFYKYGAIRDIDLKNRRGGPPFAFVEFEDPRDAEDAVYARDGYDYDGYRLRVEFPR 89          
BLAST of Arginine:serine rich splicing factor vs. UniProt/SwissProt
Match: sp|Q6NYA0|SRS1B_DANRE (Serine/arginine-rich splicing factor 1B OS=Danio rerio OX=7955 GN=srsf1b PE=2 SV=1)

HSP 1 Score: 95.5153 bits (236), Expect = 8.392e-22
Identity = 41/81 (50.62%), Postives = 60/81 (74.07%), Query Frame = 3
Query:  483 PPKSTDYRVFISNIPPYSNWQDIKDFMRRYGDVSYADVFGNGTGVCDFVSKEDFRYVLKYVNNVKFKTVSGQTSYIKVEED 725
            P + ++YRV +S +PP  +WQD+KD MR  GDV YADVF +GTGV +FV KED  Y ++ ++N KF++  G+T+YI+V+ D
Sbjct:  114 PSRRSEYRVIVSGLPPSGSWQDLKDHMREAGDVCYADVFRDGTGVVEFVRKEDMTYAVRKLDNTKFRSHEGETAYIRVKVD 194          

HSP 2 Score: 77.411 bits (189), Expect = 2.792e-15
Identity = 48/80 (60.00%), Postives = 62/80 (77.50%), Query Frame = 3
Query:   42 PIMSNERKIYVGNLPPDSTSRDIEKLFSCFGKIIDIDIKN-RNGPPFAFIQFXXXXXXXXXXXXXXNYDLDGFSLRVEFP 278
            P  +N+ +IYVGNLPPD  ++D+E +F  +G I DID+KN R GPPFAF++F+D RDAEDA+  RD YD DG+ LRVEFP
Sbjct:    9 PAGNNDCRIYVGNLPPDIRTKDVEDVFYKYGAIRDIDLKNRRGGPPFAFVEFEDPRDAEDAVYGRDGYDYDGYRLRVEFP 88          
BLAST of Arginine:serine rich splicing factor vs. TrEMBL
Match: A0A0R3STR2 (Uncharacterized protein OS=Hymenolepis diminuta OX=6216 GN=HDID_LOCUS8820 PE=4 SV=1)

HSP 1 Score: 155.221 bits (391), Expect = 3.223e-42
Identity = 93/226 (41.15%), Postives = 132/226 (58.41%), Query Frame = 3
Query:   48 MSNERKIYVGNLPPDSTSRDIEKLFSCFGKIIDIDIKNRNGPPFAFIQFXXXXXXXXXXXXXXNYDLDGFSLRVEFPREIKQNKKDYKEYSRFENPVYVPPFDPYDMYLPYNYGPFPRQDRFXXXXXXXXXXXXXXXXXXXXXPRPPKSTDYRVFISNIPPYSNWQDIKDFMRRYGDVSYADVFGNGTGVCDFVSKEDFRYVLKYVNNVKFKTVSGQTSYIKVEED 725
            M  +RKIY+GNLPPD  S+D++ +FS +G+I DID+K R GPPFAFI+F+D RDAEDA+R+ D Y L+G++LRVEFPR    +         F      P F                                      +R   P + +DYRV ++N+PP  +WQD+KD MR  GDV +ADVF +G+GV +F+  ED +Y LK +++ KF++  G+TSYI+V E+
Sbjct:    1 MKMDRKIYIGNLPPDVRSKDLDSVFSKYGRISDIDLKRRRGPPFAFIEFEDERDAEDAVRDCDGYKLNGYALRVEFPRASNNS---------FRRGGAAPGF--------------------------------------SRGSGPSRRSDYRVLVTNLPPSGSWQDLKDHMREAGDVGFADVFKDGSGVVEFLRYEDMKYALKKLDDSKFRSHEGETSYIRVREE 179          
BLAST of Arginine:serine rich splicing factor vs. TrEMBL
Match: A0A0R3TNU7 (Uncharacterized protein OS=Rodentolepis nana OX=102285 GN=HNAJ_LOCUS9084 PE=4 SV=1)

HSP 1 Score: 154.836 bits (390), Expect = 3.744e-42
Identity = 93/226 (41.15%), Postives = 131/226 (57.96%), Query Frame = 3
Query:   48 MSNERKIYVGNLPPDSTSRDIEKLFSCFGKIIDIDIKNRNGPPFAFIQFXXXXXXXXXXXXXXNYDLDGFSLRVEFPREIKQNKKDYKEYSRFENPVYVPPFDPYDMYLPYNYGPFPRQDRFXXXXXXXXXXXXXXXXXXXXXPRPPKSTDYRVFISNIPPYSNWQDIKDFMRRYGDVSYADVFGNGTGVCDFVSKEDFRYVLKYVNNVKFKTVSGQTSYIKVEED 725
            M  +RKIY+GNLPPD  S+D++ +FS +G+I DID+K R GPPFAFI+F+D RDAEDA+R+ D Y L+G++LRVEFPR              F      P F                                      +R   P + +DYRV ++N+PP  +WQD+KD MR  GDV +ADVF +G+GV +F+  ED +Y LK +++ KF++  G+TSYI+V E+
Sbjct:    1 MKMDRKIYIGNLPPDVRSKDLDSVFSKYGRISDIDLKRRRGPPFAFIEFEDERDAEDAVRDCDGYKLNGYALRVEFPRASNNG---------FRRGGAAPGF--------------------------------------SRGSGPSRRSDYRVLVTNLPPSGSWQDLKDHMREAGDVGFADVFKDGSGVVEFLRYEDMKYALKKLDDSKFRSHEGETSYIRVREE 179          
BLAST of Arginine:serine rich splicing factor vs. TrEMBL
Match: A0A3Q4BTH6 (Uncharacterized protein OS=Mola mola OX=94237 PE=4 SV=1)

HSP 1 Score: 149.828 bits (377), Expect = 9.153e-40
Identity = 97/229 (42.36%), Postives = 128/229 (55.90%), Query Frame = 3
Query:   42 PIMSNERKIYVGNLPPDSTSRDIEKLFSCFGKIIDIDIKNR-NGPPFAFIQFXXXXXXXXXXXXXXNYDLDGFSLRVEFPREIKQNKKDYKEYSRFENPVYVPPFDPYDMYLPYNYGPFPRQDRFXXXXXXXXXXXXXXXXXXXXXPRPPKSTDYRVFISNIPPYSNWQDIKDFMRRYGDVSYADVFGNGTGVCDFVSKEDFRYVLKYVNNVKFKTVSGQTSYIKVEED 725
            P  +N+ +IYVGNLPPD  S+DIE LF  +G I DID+KNR  GPPFAF+QF+D RD+EDA+ ERD YD DG+ LRVEFPR  +              P                YGP                              P + ++YRV +S +PP  +WQD+KD MR  GDV YADV+ +GTGV +FV KED  Y ++ ++N KF++  G+T+YI V+ D
Sbjct:    9 PSGTNDCRIYVGNLPPDIRSKDIEDLFYKYGAIRDIDLKNRRGGPPFAFVQFEDPRDSEDAVYERDGYDYDGYRLRVEFPRSGRGGGGGGGGGGGMGPPRT-------------RYGP------------------------------PSRRSEYRVLVSGLPPSGSWQDLKDHMREAGDVCYADVYRDGTGVVEFVRKEDMSYAVRKLDNTKFRSHEGETAYIHVKVD 194          
BLAST of Arginine:serine rich splicing factor vs. TrEMBL
Match: A0A4W6D9H0 (Uncharacterized protein OS=Lates calcarifer OX=8187 GN=LOC108893547 PE=4 SV=1)

HSP 1 Score: 149.443 bits (376), Expect = 1.193e-39
Identity = 98/229 (42.79%), Postives = 130/229 (56.77%), Query Frame = 3
Query:   42 PIMSNERKIYVGNLPPDSTSRDIEKLFSCFGKIIDIDIKNR-NGPPFAFIQFXXXXXXXXXXXXXXNYDLDGFSLRVEFPREIKQNKKDYKEYSRFENPVYVPPFDPYDMYLPYNYGPFPRQDRFXXXXXXXXXXXXXXXXXXXXXPRPPKSTDYRVFISNIPPYSNWQDIKDFMRRYGDVSYADVFGNGTGVCDFVSKEDFRYVLKYVNNVKFKTVSGQTSYIKVEED 725
            P  SN+ +IYVGNLPPD  S+D+E LF  +G I DID+KNR  GPPFAF+QF+D RDAEDA+  RD YD DG+ LRVEFPR  +             +    PP           YGP                              P + ++YRV +S +PP  +WQD+KD MR  GDV YADV+ +GTGV +FV KED  Y ++ ++N KF++  G+T+YI+V+ D
Sbjct:   10 PAGSNDCRIYVGNLPPDIRSKDVEDLFYKYGSIRDIDLKNRRGGPPFAFVQFEDPRDAEDAVYGRDGYDYDGYRLRVEFPRSGRGGGS---------SGAMGPPRG--------RYGP------------------------------PSRRSEYRVVVSGLPPSGSWQDLKDHMREAGDVCYADVYRDGTGVVEFVRKEDMTYAVRKLDNTKFRSHEGETAYIRVKMD 191          
BLAST of Arginine:serine rich splicing factor vs. TrEMBL
Match: A0A023FXP3 (Putative alternative splicing factor asf/sf2 rrm superfamily OS=Amblyomma parvum OX=251391 PE=2 SV=1)

HSP 1 Score: 148.288 bits (373), Expect = 1.674e-39
Identity = 96/225 (42.67%), Postives = 124/225 (55.11%), Query Frame = 3
Query:   51 SNERKIYVGNLPPDSTSRDIEKLFSCFGKIIDIDIKNRNGPPFAFIQFXXXXXXXXXXXXXXNYDLDGFSLRVEFPREIKQNKKDYKEYSRFENPVYVPPFDPYDMYLPYNYGPFPRQDRFXXXXXXXXXXXXXXXXXXXXXPRPPKSTDYRVFISNIPPYSNWQDIKDFMRRYGDVSYADVFGNGTGVCDFVSKEDFRYVLKYVNNVKFKTVSGQTSYIKVEED 725
             NE +IYVGNLPPD  S+DIE LF  FGKI  ID+KNR GPPFAF++FDD RDAEDA+  RD YD DG+ LRVEFPR     +                     +M      GP  R+                              + YRV +S +PP  +WQD+KD MR  GDV YADVF +GTGV +F+  ED +Y +K +++ +F++  G+ SYI+V ED
Sbjct:    5 GNECRIYVGNLPPDIRSKDIEDLFYKFGKITFIDLKNRRGPPFAFVEFDDPRDAEDAVHARDGYDYDGYRLRVEFPRGSAPGRG--------------------NMGPGRGRGPPARR------------------------------SQYRVLVSGLPPSGSWQDLKDHMREAGDVCYADVFKDGTGVVEFLRYEDMKYAVKKLDDSRFRSHEGEVSYIRVRED 179          
BLAST of Arginine:serine rich splicing factor vs. Ensembl Cavefish
Match: srsf1b (serine and arginine rich splicing factor 1b [Source:NCBI gene;Acc:393565])

HSP 1 Score: 96.2857 bits (238), Expect = 2.769e-23
Identity = 41/81 (50.62%), Postives = 60/81 (74.07%), Query Frame = 3
Query:  483 PPKSTDYRVFISNIPPYSNWQDIKDFMRRYGDVSYADVFGNGTGVCDFVSKEDFRYVLKYVNNVKFKTVSGQTSYIKVEED 725
            P + ++YRV +S +PP  +WQD+KD MR  GDV YADVF +GTGV +FV KED  Y ++ ++N KF++  G+T+YI+V+ D
Sbjct:   85 PSRRSEYRVIVSGLPPSGSWQDLKDHMREAGDVCYADVFRDGTGVVEFVRKEDMTYAVRKLDNTKFRSHEGETAYIRVKVD 165          
BLAST of Arginine:serine rich splicing factor vs. Ensembl Cavefish
Match: srsf1a (serine and arginine rich splicing factor 1 [Source:NCBI gene;Acc:103029385])

HSP 1 Score: 96.2857 bits (238), Expect = 6.113e-23
Identity = 41/81 (50.62%), Postives = 60/81 (74.07%), Query Frame = 3
Query:  483 PPKSTDYRVFISNIPPYSNWQDIKDFMRRYGDVSYADVFGNGTGVCDFVSKEDFRYVLKYVNNVKFKTVSGQTSYIKVEED 725
            P + ++YRV +S +PP  +WQD+KD MR  GDV YADVF +GTGV +FV KED  Y ++ ++N KF++  G+T+YI+V+ D
Sbjct:  119 PSRRSEYRVIVSGLPPSGSWQDLKDHMREAGDVCYADVFRDGTGVVEFVRKEDMTYAVRKLDNTKFRSHEGETAYIRVKMD 199          

HSP 2 Score: 81.2629 bits (199), Expect = 1.588e-17
Identity = 50/81 (61.73%), Postives = 63/81 (77.78%), Query Frame = 3
Query:   42 PIMSNERKIYVGNLPPDSTSRDIEKLFSCFGKIIDIDIKN-RNGPPFAFIQFXXXXXXXXXXXXXXNYDLDGFSLRVEFPR 281
            P  SN+ +IYVGNLPPD  ++D+E +F  +G I DID+KN R GPPFAF++F+D RDAEDA+  RD YD DG+ LRVEFPR
Sbjct:    9 PAGSNDCRIYVGNLPPDIRTKDVEDVFYKYGAIRDIDLKNRRGGPPFAFVEFEDPRDAEDAVYARDGYDYDGYRLRVEFPR 89          
BLAST of Arginine:serine rich splicing factor vs. Ensembl Cavefish
Match: srsf9 (serine/arginine-rich splicing factor 9 [Source:NCBI gene;Acc:103033517])

HSP 1 Score: 87.0409 bits (214), Expect = 1.202e-19
Identity = 33/82 (40.24%), Postives = 58/82 (70.73%), Query Frame = 3
Query:  489 KSTDYRVFISNIPPYSNWQDIKDFMRRYGDVSYADVFGNGTGVCDFVSKEDFRYVLKYVNNVKFKTVSGQTSYIKVEEDKTT 734
            + +++RV ++ +PP  +WQD+KD MR  GDV +ADV  +G GV +F+ +ED  Y L+ ++  +F++  G+T+YI+V E++ T
Sbjct:  103 RRSEFRVIVTGLPPTGSWQDLKDHMREAGDVCFADVQRDGEGVVEFLRREDMEYALRRLDGTEFRSHQGETAYIRVLEERGT 184          

HSP 2 Score: 58.5362 bits (140), Expect = 1.101e-9
Identity = 41/73 (56.16%), Postives = 51/73 (69.86%), Query Frame = 3
Query:   63 KIYVGNLPPDSTSRDIEKLFSCFGKIIDIDIKNRNGP-PFAFIQFXXXXXXXXXXXXXXNYDLDGFSLRVEFP 278
            +IYVGNLP D   RDIE LF  +GKI DI++KN  G  PFAF++F+D RDAEDA+  R+ Y      LRVE+P
Sbjct:    5 RIYVGNLPMDVQERDIEDLFFKYGKIRDIELKNNRGTIPFAFVRFEDPRDAEDAVYGRNGYGFGDCKLRVEYP 77          
BLAST of Arginine:serine rich splicing factor vs. Ensembl Cavefish
Match: srsf5b (serine/arginine-rich splicing factor 5 [Source:NCBI gene;Acc:103029514])

HSP 1 Score: 68.1662 bits (165), Expect = 7.356e-13
Identity = 63/218 (28.90%), Postives = 98/218 (44.95%), Query Frame = 3
Query:   63 KIYVGNLPPDSTSRDIEKLFSCFGKIIDIDIKNRNGPPFAFIQFXXXXXXXXXXXXXXNYDLDGFSLRVEFPREIKQNKKDYKEYSRFENPVYVPPFDPYDMYLPYNYGPFPRQDRFXXXXXXXXXXXXXXXXXXXXXPRPPKSTDYRVFISNIPPYSNWQDIKDFMRRYGDVSYADVF--GNGTGVCDFVSKEDFRYVL-----KYVNNVKFKTVSG 695
            +I++G L P +  +D+E+ F  +G+I DID+K      F F++FDD RDAEDA+     Y+LDG  L          N++   E++R    +            P  YG   +  R +                      PP  T+ R+ + N+    +WQD+KDFMR+ G+V++AD        GV +F S  D +  L     K +N  K K +  
Sbjct:    5 RIFIGRLNPSAREKDVERFFKGYGRIRDIDLKR----GFGFVEFDDPRDAEDAV-----YELDGKEL---------CNERVTIEHARVR--LRGGRGRGGGGRFPDRYGRGSQDSRRNP---------------------PPMRTENRLIVENLSSRVSWQDLKDFMRQAGEVTFADAHRPKLNEGVVEFASHSDLKNALEKLSGKEINGRKIKLIEA 181          
BLAST of Arginine:serine rich splicing factor vs. Ensembl Cavefish
Match: srsf5b (serine/arginine-rich splicing factor 5 [Source:NCBI gene;Acc:103029514])

HSP 1 Score: 68.1662 bits (165), Expect = 7.356e-13
Identity = 63/218 (28.90%), Postives = 98/218 (44.95%), Query Frame = 3
Query:   63 KIYVGNLPPDSTSRDIEKLFSCFGKIIDIDIKNRNGPPFAFIQFXXXXXXXXXXXXXXNYDLDGFSLRVEFPREIKQNKKDYKEYSRFENPVYVPPFDPYDMYLPYNYGPFPRQDRFXXXXXXXXXXXXXXXXXXXXXPRPPKSTDYRVFISNIPPYSNWQDIKDFMRRYGDVSYADVF--GNGTGVCDFVSKEDFRYVL-----KYVNNVKFKTVSG 695
            +I++G L P +  +D+E+ F  +G+I DID+K      F F++FDD RDAEDA+     Y+LDG  L          N++   E++R    +            P  YG   +  R +                      PP  T+ R+ + N+    +WQD+KDFMR+ G+V++AD        GV +F S  D +  L     K +N  K K +  
Sbjct:    5 RIFIGRLNPSAREKDVERFFKGYGRIRDIDLKR----GFGFVEFDDPRDAEDAV-----YELDGKEL---------CNERVTIEHARVR--LRGGRGRGGGGRFPDRYGRGSQDSRRNP---------------------PPMRTENRLIVENLSSRVSWQDLKDFMRQAGEVTFADAHRPKLNEGVVEFASHSDLKNALEKLSGKEINGRKIKLIEA 181          
BLAST of Arginine:serine rich splicing factor vs. Ensembl Sea Lamprey
Match: SRSF5 (serine and arginine rich splicing factor 5 [Source:HGNC Symbol;Acc:HGNC:10787])

HSP 1 Score: 71.2478 bits (173), Expect = 1.681e-14
Identity = 63/224 (28.12%), Postives = 107/224 (47.77%), Query Frame = 3
Query:   63 KIYVGNLPPDSTSRDIEKLFSCFGKIIDIDIKNRNGPPFAFIQFXXXXXXXXXXXXXXNYDLDGFSLRVEFPREIKQNKKDYKEYSRFENPVYVPPFDPYDMYLPYNYGPFPRQDRFXXXXXXXXXXXXXXXXXXXXXPRPPKSTDYRVFISNIPPYSNWQDIKDFMRRYGDVSYADV--FGNGTGVCDFVSKEDFRYVLKYVNNVKFKTVSGQTSYIKVEEDK 728
            ++++  L P   ++D+EK F  +GKI DIDIK      F F++FDD RDA+DA+ + +  +L+   + VE  R  +                     D  D ++    GP P           R      + +    R  PP  TD+R+ + N+    +WQD+KD+MR+ G+V++AD        GV +F ++ D +  +  +N    K ++G+   + VEE+K
Sbjct:    6 RVFIARLGPHVRTKDVEKFFKGYGKIRDIDIK----AGFGFVEFDDCRDADDAVSDLNGRELNNERMTVEHARAPRARG------------------DRMDTFVRRPSGPPP-------TYRPRGGGPSSSSASSGGRYGPPSRTDHRLVVENLSSTVSWQDLKDYMRQAGEVTFADAHRIHQNVGVVEFATRNDMKNAIDKLNG---KEINGRKIKL-VEENK 196          
BLAST of Arginine:serine rich splicing factor vs. Ensembl Sea Lamprey
Match: ENSPMAT00000010377.1 (pep scaffold:Pmarinus_7.0:GL481287:28:8451:-1 gene:ENSPMAG00000009397.1 transcript:ENSPMAT00000010377.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 62.7734 bits (151), Expect = 1.276e-11
Identity = 27/63 (42.86%), Postives = 42/63 (66.67%), Query Frame = 3
Query:  483 PPKSTDYRVFISNIPPYSNWQDIKDFMRRYGDVSYADVFGNGTGVCDFVSKEDFRYVLKYVNN 671
            PP  TDYR+ + N+    +WQD+KD+MR+ G+V+YADV G+  GV +F++  D R  L  ++ 
Sbjct:  104 PPPRTDYRLVVENVSSRCSWQDLKDYMRQAGEVTYADVRGD-EGVVEFLTYADMRRALDKLDG 165          
BLAST of Arginine:serine rich splicing factor vs. Ensembl Sea Lamprey
Match: ENSPMAT00000006373.1 (pep scaffold:Pmarinus_7.0:GL478975:164155:166212:-1 gene:ENSPMAG00000005755.1 transcript:ENSPMAT00000006373.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 46.2098 bits (108), Expect = 3.768e-6
Identity = 37/92 (40.22%), Postives = 55/92 (59.78%), Query Frame = 3
Query:   63 KIYVGNLPPDSTSRDIEKLFSCFGKIIDIDIKNRNGPPFAFIQFXXXXXXXXXXXXXXNYDLDGFSLRVEFPREIKQNKKDYKE---YSRFE 329
            K+YVGNL   +T  D+EK+FS +G +  + +  RN P FAF+QF+D RDA D+ RE D   + G  +RVE     ++ K+ +     +SR E
Sbjct:   16 KVYVGNLGSGATEYDLEKVFSYYGPLRSVWVA-RNPPGFAFVQFEDARDARDSCRELDGRLVCGSRVRVEMSTGERRQKRGWSAQPPWSRCE 106          
BLAST of Arginine:serine rich splicing factor vs. Ensembl Yeast
Match: NPL3 (RNA-binding protein; promotes elongation, regulates termination, and carries poly(A) mRNA from nucleus to cytoplasm; represses translation initiation by binding eIF4G; required for pre-mRNA splicing; interacts with E3 ubiquitin ligase Bre1p, linking histone ubiquitination to mRNA processing; may have role in telomere maintenance; dissociation from mRNAs promoted by Mtr10p; phosphorylated by Sky1p in cytoplasm; protein abundance increases in response to DNA replication stress [Source:SGD;Acc:S000002840])

HSP 1 Score: 48.1358 bits (113), Expect = 9.430e-7
Identity = 26/79 (32.91%), Postives = 43/79 (54.43%), Query Frame = 3
Query:  501 YRVFISNIPPYSNWQDIKDFMRRYG-DVSYADVFG---NGTGVCDFVSKEDFRYVLKYVNNVKFKTVSGQTSYIKVEED 725
            YR+ + N+P   +WQD+KD  R    + +++ V     +GTG  +F S+E     L+ +NN++F+      S I VE D
Sbjct:  200 YRITMKNLPEGCSWQDLKDLARENSLETTFSSVNTRDFDGTGALEFPSEEILVEALERLNNIEFR-----GSVITVERD 273          
BLAST of Arginine:serine rich splicing factor vs. Ensembl Nematostella
Match: EDO44646 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RVI6])

HSP 1 Score: 120.553 bits (301), Expect = 8.209e-33
Identity = 80/227 (35.24%), Postives = 119/227 (52.42%), Query Frame = 3
Query:   51 SNERKIYVGNLPPDSTSRDIEKLFSCFGKIIDIDIKNRN--GPPFAFIQFXXXXXXXXXXXXXXNYDLDGFSLRVEFPREIKQNKKDYKEYSRFENPVYVPPFDPYDMYLPYNYGPFPRQDRFXXXXXXXXXXXXXXXXXXXXXPRPPKSTDYRVFISNIPPYSNWQDIKDFMRRYGDVSYADVFGNGTGVCDFVSKEDFRYVLKYVNNVKFKTVSGQTSYIKVEED 725
            SN+ ++YVGNLP D   +D+  +F  +G I D+D+KNR   GPPFAF++F+D RDAEDA++ RD ++ DG+ +RVEFPR                                   GP PR+  F                              RV +S +PP  +WQD+KD MR  GDV + DVF +GTGV +F   +D ++ ++ +++ KF++  G+TSYI+V+ D
Sbjct:    6 SNDCRVYVGNLPQDVREKDLHDIFYKYGHIADVDLKNRRGAGPPFAFVEFEDPRDAEDAVKGRDGHEFDGYRIRVEFPRG--------------------------------GSGPPPRRSDF------------------------------RVQVSGLPPTGSWQDLKDHMREAGDVLFTDVFKDGTGVVEFARYDDMKFAIRNLDDSKFRSHEGETSYIRVKSD 170          
BLAST of Arginine:serine rich splicing factor vs. Ensembl Nematostella
Match: EDO47776 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RL13])

HSP 1 Score: 80.1073 bits (196), Expect = 5.441e-18
Identity = 65/214 (30.37%), Postives = 101/214 (47.20%), Query Frame = 3
Query:   48 MSNER-KIYVGNLPPDSTSRDIEKLFSCFGKIIDIDIKNRNGPPFAFIQFXXXXXXXXXXXXXXNYDLDGFSLRVEFPREIKQNKKDYKEYSRFENPVYVPPFDPYDMYLPYNYGPFPRQDRFXXXXXXXXXXXXXXXXXXXXXPRPPKSTDYRVFISNIPPYSNWQDIKDFMRRYGDVSYADVFGN--GTGVCDFVSKEDFRYVLKYVNNVKF 680
            MS E  +++VG LPP +  RD+EK F  FG+I DI++K      FAF++FDD RDA+DA+ E ++ +L G  + VE                        P                PR+DR+                       PP +T+YR+ + N+   + WQD+KD+MR+ G+V++     +  G GVCDF ++ D  Y LK ++  + 
Sbjct:    1 MSREGGRVFVGRLPPRANDRDLEKFFKGFGRINDINLKQ----GFAFVEFDDARDADDAVYELNHKELLGERVSVEHAH---------------------PSRGGGGGGGRGFRDRSPRRDRY-----------------------PPYNTEYRLIVENLSTRAGWQDLKDYMRQAGEVTFTQCHKDRVGEGVCDFSNESDMLYALKKLDGTEL 166          
BLAST of Arginine:serine rich splicing factor vs. Ensembl Nematostella
Match: EDO31620 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SXE8])

HSP 1 Score: 68.1662 bits (165), Expect = 9.020e-14
Identity = 48/208 (23.08%), Postives = 87/208 (41.83%), Query Frame = 3
Query:   63 KIYVGNLPPDSTSRDIEKLFSCFGKIIDIDIKNRNGPPFAFIQFXXXXXXXXXXXXXXNYDLDGFSLRVEFPREIKQNKKDYKEYSRFENPVYVPPFDPYDMYLPYNYGPFPRQDRFXXXXXXXXXXXXXXXXXXXXXPRPPKSTDYRVFISNIPPYSNWQDIKDFMRRYGDVSYADVFGN--GTGVCDFVSKEDFRYVLKYVNNVKF 680
            K+Y G LP  +T +D+E L   FGK+ ++D K      +A++ F + +DA+ A+   +N +  G  + +E  +E++     Y                         Y    RQ                          PP  +++RV + N+   + W ++K+FM   G+V YAD      G GV +F ++ED +  +  ++  +F
Sbjct:    4 KVYCGRLPATATEKDLENLVKVFGKVREVDFKE----GYAYVVFKENKDADRAVAALNNSEFHGAKILMEKAKEMRNGVGGYTAAG--------------------GYTARTRQ------------------------MGPPVRSEFRVIVENLSTRAKWLELKEFMNNAGEVCYADTHRRRPGEGVVEFTTEEDMKRAIASLDKCEF 163          
BLAST of Arginine:serine rich splicing factor vs. Ensembl Nematostella
Match: EDO32443 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SV28])

HSP 1 Score: 61.2326 bits (147), Expect = 2.519e-11
Identity = 25/68 (36.76%), Postives = 45/68 (66.18%), Query Frame = 3
Query:  483 PPKSTDYRVFISNIPPYSNWQDIKDFMRRYGDVSYADVFG--NGTGVCDFVSKEDFRYVLKYVNNVKF 680
            PP  T++R+ + N+   SNWQD+K++M + G+V++AD      G GV +F +K+D +  LK +++ +F
Sbjct:  101 PPIRTEHRLAVENLSSRSNWQDLKEYMGKVGEVTFADAHKRRQGEGVVEFATKDDMKTALKKLDDTEF 168          
BLAST of Arginine:serine rich splicing factor vs. Ensembl Nematostella
Match: EDO39290 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SAJ5])

HSP 1 Score: 58.5362 bits (140), Expect = 2.430e-10
Identity = 30/84 (35.71%), Postives = 48/84 (57.14%), Query Frame = 3
Query:  483 PPKSTDYRVFISNIPPYSNWQDIKDFMRRYGDVSYADVFGN--GTGVCDFVSKEDFRYVLKYVNNVKFKTVSGQTSYIKVEEDK 728
            PP  T+Y V + N+   ++WQD+KD+ R+YG V+YAD      G GV +F SK+D    ++ +++ +          I+V EDK
Sbjct:  101 PPVRTNYSVIVENLSSRTSWQDLKDYFRKYGKVTYADAHKKRIGEGVVEFESKDDLNTAIEKLDDTEL-----GGRRIRVYEDK 179          
BLAST of Arginine:serine rich splicing factor vs. Ensembl Medaka
Match: srsf1a (serine/arginine-rich splicing factor 1 [Source:NCBI gene;Acc:101162697])

HSP 1 Score: 138.658 bits (348), Expect = 6.290e-39
Identity = 90/229 (39.30%), Postives = 128/229 (55.90%), Query Frame = 3
Query:   42 PIMSNERKIYVGNLPPDSTSRDIEKLFSCFGKIIDIDIKN-RNGPPFAFIQFXXXXXXXXXXXXXXNYDLDGFSLRVEFPREIKQNKKDYKEYSRFENPVYVPPFDPYDMYLPYNYGPFPRQDRFXXXXXXXXXXXXXXXXXXXXXPRPPKSTDYRVFISNIPPYSNWQDIKDFMRRYGDVSYADVFGNGTGVCDFVSKEDFRYVLKYVNNVKFKTVSGQTSYIKVEED 725
            P  +N+ +IYVGNLPPD  ++D+E +F  +G I DID+KN R GPPFAFI+F+D RDA+DA+  RD YD DG+ LRVEFPR  + ++  +          Y PP                                              + ++YRV +S +P   +WQD+KD MR  GDV YADV+ +GTGV +FV KED  Y ++ ++N KF++  G+T+YI+V+ D
Sbjct:    8 PAGNNDCRIYVGNLPPDIRTKDVEDVFYKYGTIRDIDLKNRRGGPPFAFIEFEDPRDADDAVYGRDGYDYDGYRLRVEFPRSGRGSRGGFGGIGGAPRGRYGPP---------------------------------------------SRRSEYRVVVSGLPQSGSWQDLKDHMREAGDVCYADVYRDGTGVVEFVRKEDMTYAVRKLDNTKFRSHEGETAYIRVKLD 191          
BLAST of Arginine:serine rich splicing factor vs. Ensembl Medaka
Match: ENSORLT00000038036.1 (serine/arginine-rich splicing factor 1B [Source:NCBI gene;Acc:101171767])

HSP 1 Score: 135.191 bits (339), Expect = 1.269e-37
Identity = 99/247 (40.08%), Postives = 126/247 (51.01%), Query Frame = 3
Query:   42 PIMSNERKIYVGNLPPDSTSRDIEKLFSCFGKIIDIDIKN-RNGPPFAFIQFXXXXXXXXXXXXXXNYDLDGFSLRVEFPREIKQNKKDYKEYSRFENPVYVPPFDPYDMYLPYNYGPFPRQDRFXXXXXXXXXXXXXXXXXXXXXPRPPKSTDYRVFISNIPPYSNWQDIKDFMRRYGDVSYADVFGNGTGVCDFVSKEDFRYVLKYVNNVKFKT--VSGQTSYIKVEEDKTTKRGYHRRSPRTHN 773
            P  SN+ +IYVGNLPPD  S+D+E LF  +G I DID+KN R GPPFAF+QF+D RDAEDA+  RD YD DG+ LRVEFPR            S                     YGP  R+                              ++ RV +S +PP  +WQD+KD MR  GDV YADVF +GTGV +FV KED  Y ++ ++N KF++   S +   +  EE    K    RRS   H 
Sbjct:    9 PAGSNDCRIYVGNLPPDIRSKDVEDLFYKYGSIRDIDLKNRRGGPPFAFVQFEDPRDAEDAVYGRDGYDYDGYRLRVEFPR------------SGRGGGGGGGGGGGGMGPPRGRYGPPSRR------------------------------SENRVIVSGLPPSGSWQDLKDHMREAGDVCYADVFRDGTGVVEFVRKEDMTYAVRKLDNTKFRSHESSEEQDRMGREETVHVKINRRRRSQIWHQ 213          
BLAST of Arginine:serine rich splicing factor vs. Ensembl Medaka
Match: srsf9 (serine/arginine-rich splicing factor 9 [Source:NCBI gene;Acc:101164733])

HSP 1 Score: 114.005 bits (284), Expect = 1.466e-29
Identity = 76/225 (33.78%), Postives = 112/225 (49.78%), Query Frame = 3
Query:   63 KIYVGNLPPDSTSRDIEKLFSCFGKIIDIDIKNRNGP-PFAFIQFXXXXXXXXXXXXXXNYDLDGFSLRVEFPREIKQNKKDYKEYSRFENPVYVPPFDPYDMYLPYNYGPFPRQDRFXXXXXXXXXXXXXXXXXXXXXPRPPKSTDYRVFISNIPPYSNWQDIKDFMRRYGDVSYADVFGNGTGVCDFVSKEDFRYVLKYVNNVKFKTVSGQTSYIKVEEDKTT 734
            +IY+GNLP D   RDIE LF  +GKI +I++KN  G  PFAFI+F+D RDA+DA+  R+ Y      LRVE+PR                   + PP                                              + +++RV ++ +PP  +WQD+KD MR  GDV +ADV  +G GV +F+ +ED  Y L+ ++  +F++  G+TSYI+V ED+  
Sbjct:    5 RIYIGNLPIDVQERDIEDLFFKYGKIREIELKNNRGTIPFAFIRFEDPRDADDAVYGRNGYVYGNSKLRVEYPRSTGGKPTPMGPGGAGPRGRFGPP---------------------------------------------TRRSEFRVIVTGLPPTGSWQDLKDHMREAGDVCFADVQRDGEGVVEFLRREDMEYALRRLDRTEFRSHQGETSYIRVFEDRGA 184          
BLAST of Arginine:serine rich splicing factor vs. Ensembl Medaka
Match: srsf9 (serine/arginine-rich splicing factor 9 [Source:NCBI gene;Acc:101164733])

HSP 1 Score: 99.7525 bits (247), Expect = 3.179e-24
Identity = 71/216 (32.87%), Postives = 105/216 (48.61%), Query Frame = 3
Query:   63 KIYVGNLPPDSTSRDIEKLFSCFGKIIDIDIKNRNGP-PFAFIQFXXXXXXXXXXXXXXNYDLDGFSLRVEFPREIKQNKKDYKEYSRFENPVYVPPFDPYDMYLPYNYGPFPRQDRFXXXXXXXXXXXXXXXXXXXXXPRPPKSTDYRVFISNIPPYSNWQDIKDFMRRYGDVSYADVFGNGTGVCDFVSKEDFRYVLKYVNNVKFKT--VSGQT 701
            +IY+GNLP D   RDIE LF  +GKI +I++KN  G  PFAFI+F+D RDA+DA+  R+ Y      LRVE+PR                   + PP                                              + +++RV ++ +PP  +WQD+KD MR  GDV +ADV  +G GV +F+ +ED  Y L+ ++  +F++  V GQ+
Sbjct:   15 RIYIGNLPIDVQERDIEDLFFKYGKIREIELKNNRGTIPFAFIRFEDPRDADDAVYGRNGYVYGNSKLRVEYPRSTGGKPTPMGPGGAGPRGRFGPPT---------------------------------------------RRSEFRVIVTGLPPTGSWQDLKDHMREAGDVCFADVQRDGEGVVEFLRREDMEYALRRLDRTEFRSHQVIGQS 185          
BLAST of Arginine:serine rich splicing factor vs. Ensembl Medaka
Match: ENSORLT00000006699.2 (serine/arginine-rich splicing factor 1B [Source:NCBI gene;Acc:101171767])

HSP 1 Score: 93.5893 bits (231), Expect = 6.004e-22
Identity = 40/81 (49.38%), Postives = 59/81 (72.84%), Query Frame = 3
Query:  483 PPKSTDYRVFISNIPPYSNWQDIKDFMRRYGDVSYADVFGNGTGVCDFVSKEDFRYVLKYVNNVKFKTVSGQTSYIKVEED 725
            P + ++ RV +S +PP  +WQD+KD MR  GDV YADVF +GTGV +FV KED  Y ++ ++N KF++  G+T+YI+V+ D
Sbjct:  115 PSRRSENRVIVSGLPPSGSWQDLKDHMREAGDVCYADVFRDGTGVVEFVRKEDMTYAVRKLDNTKFRSHEGETAYIRVKTD 195          

HSP 2 Score: 84.7297 bits (208), Expect = 1.021e-18
Identity = 53/81 (65.43%), Postives = 63/81 (77.78%), Query Frame = 3
Query:   42 PIMSNERKIYVGNLPPDSTSRDIEKLFSCFGKIIDIDIKN-RNGPPFAFIQFXXXXXXXXXXXXXXNYDLDGFSLRVEFPR 281
            P  SN+ +IYVGNLPPD  S+D+E LF  +G I DID+KN R GPPFAF+QF+D RDAEDA+  RD YD DG+ LRVEFPR
Sbjct:    9 PAGSNDCRIYVGNLPPDIRSKDVEDLFYKYGSIRDIDLKNRRGGPPFAFVQFEDPRDAEDAVYGRDGYDYDGYRLRVEFPR 89          
BLAST of Arginine:serine rich splicing factor vs. Planmine SMEST
Match: SMESG000013050.1 (SMESG000013050.1)

HSP 1 Score: 408.683 bits (1049), Expect = 3.506e-144
Identity = 242/242 (100.00%), Postives = 242/242 (100.00%), Query Frame = 3
Query:   48 MSNERKIYVGNLPPDSTSRDIEKLFSCFGKIIDIDIKNRNGPPFAFIQFXXXXXXXXXXXXXXNYDLDGFSLRVEFPREIKQNKKDYKEYSRFENPVYVPPFDPYDMYLPYNYGPFPRQDRFXXXXXXXXXXXXXXXXXXXXXPRPPKSTDYRVFISNIPPYSNWQDIKDFMRRYGDVSYADVFGNGTGVCDFVSKEDFRYVLKYVNNVKFKTVSGQTSYIKVEEDKTTKRGYHRRSPRTHN 773
            MSNERKIYVGNLPPDSTSRDIEKLFSCFGKIIDIDIKNRNGPPFAFIQFDDRRDAEDAIRERDNYDLDGFSLRVEFPREIKQNKKDYKEYSRFENPVYVPPFDPYDMYLPYNYGPFPRQDRFSYYGDYRRNNNYGNYSYQNRRPRPPKSTDYRVFISNIPPYSNWQDIKDFMRRYGDVSYADVFGNGTGVCDFVSKEDFRYVLKYVNNVKFKTVSGQTSYIKVEEDKTTKRGYHRRSPRTHN
Sbjct:    1 MSNERKIYVGNLPPDSTSRDIEKLFSCFGKIIDIDIKNRNGPPFAFIQFDDRRDAEDAIRERDNYDLDGFSLRVEFPREIKQNKKDYKEYSRFENPVYVPPFDPYDMYLPYNYGPFPRQDRFSYYGDYRRNNNYGNYSYQNRRPRPPKSTDYRVFISNIPPYSNWQDIKDFMRRYGDVSYADVFGNGTGVCDFVSKEDFRYVLKYVNNVKFKTVSGQTSYIKVEEDKTTKRGYHRRSPRTHN 242          
BLAST of Arginine:serine rich splicing factor vs. Planmine SMEST
Match: SMESG000013050.1 (SMESG000013050.1)

HSP 1 Score: 350.132 bits (897), Expect = 3.411e-122
Identity = 215/215 (100.00%), Postives = 215/215 (100.00%), Query Frame = 3
Query:   48 MSNERKIYVGNLPPDSTSRDIEKLFSCFGKIIDIDIKNRNGPPFAFIQFXXXXXXXXXXXXXXNYDLDGFSLRVEFPREIKQNKKDYKEYSRFENPVYVPPFDPYDMYLPYNYGPFPRQDRFXXXXXXXXXXXXXXXXXXXXXPRPPKSTDYRVFISNIPPYSNWQDIKDFMRRYGDVSYADVFGNGTGVCDFVSKEDFRYVLKYVNNVKFKTVS 692
            MSNERKIYVGNLPPDSTSRDIEKLFSCFGKIIDIDIKNRNGPPFAFIQFDDRRDAEDAIRERDNYDLDGFSLRVEFPREIKQNKKDYKEYSRFENPVYVPPFDPYDMYLPYNYGPFPRQDRFSYYGDYRRNNNYGNYSYQNRRPRPPKSTDYRVFISNIPPYSNWQDIKDFMRRYGDVSYADVFGNGTGVCDFVSKEDFRYVLKYVNNVKFKTVS
Sbjct:    1 MSNERKIYVGNLPPDSTSRDIEKLFSCFGKIIDIDIKNRNGPPFAFIQFDDRRDAEDAIRERDNYDLDGFSLRVEFPREIKQNKKDYKEYSRFENPVYVPPFDPYDMYLPYNYGPFPRQDRFSYYGDYRRNNNYGNYSYQNRRPRPPKSTDYRVFISNIPPYSNWQDIKDFMRRYGDVSYADVFGNGTGVCDFVSKEDFRYVLKYVNNVKFKTVS 215          
BLAST of Arginine:serine rich splicing factor vs. Planmine SMEST
Match: SMESG000077573.1 (SMESG000077573.1)

HSP 1 Score: 160.229 bits (404), Expect = 2.106e-47
Identity = 96/228 (42.11%), Postives = 135/228 (59.21%), Query Frame = 3
Query:   54 NERKIYVGNLPPDSTSRDIEKLFSCFGKIIDIDIKNRNGPPFAFIQFXXXXXXXXXXXXXXNYDLDGFSLRVEFPREIKQNKKDYKEYSRFENPVYVPPFDPYDMYLPYNYGPFPRQDRFXXXXXXXXXXXXXXXXXXXXXPRPPKSTDYRVFISNIPPYSNWQDIKDFMRRYGDVSYADVFGNGTGVCDFVSKEDFRYVLKYVNNVKFKTVSGQTSYIKVEEDKTTK 737
            ++RK+YVGNLPPD   RD++ LF  FGKI+DIDIK+R  PPFAFI+FDD RDAEDA+  R+ Y+ DG+ LRVEFPR   +N  ++    + E           +  L  N                                 P + +++RV++S +PP  +WQD+KD MR  GDV +ADVF +GTGVC+F+  ED +Y +K++++ KFK+  G+ SYI+VEED   K
Sbjct:    8 SKRKLYVGNLPPDIRHRDLDDLFYKFGKIVDIDIKDRKKPPFAFIEFDDPRDAEDAVTGRNGYNYDGYRLRVEFPRGNNENAGNFYSNRKAE-----------EKNLTRN-----------------------------TTSAPSRRSEFRVYVSGLPPTGSWQDLKDHMREAGDVGHADVFRDGTGVCEFLRVEDMKYAIKHLDDTKFKSHEGEISYIRVEEDCGKK 195          
BLAST of Arginine:serine rich splicing factor vs. Planmine SMEST
Match: SMESG000075205.1 (SMESG000075205.1)

HSP 1 Score: 124.02 bits (310), Expect = 1.618e-33
Identity = 77/221 (34.84%), Postives = 119/221 (53.85%), Query Frame = 3
Query:   63 KIYVGNLPPDSTSRDIEKLFSCFGKIIDIDIKNRNGPPFAFIQFXXXXXXXXXXXXXXNYDLDGFSLRVEFPREIKQNKKDYKEYSRFENPVYVPPFDPYDMYLPYNYGPFPRQDRFXXXXXXXXXXXXXXXXXXXXXPRPPKSTDYRVFISNIPPYSNWQDIKDFMRRYGDVSYADVFGNGTGVCDFVSKEDFRYVLKYVNNVKFKTVSGQTSYIKVEED 725
            K+YVGNLP D  + ++E +F  +G+I D+D+KNR    FAF++++  RDAEDAI  R+NY  +G +L+VE  +    ++   +                   Y   N G   R   F                         KST++RV +S +P  ++WQD+KD M+  GDVSYADVF +GTGVCD+++++D +Y LK +++ + KT  G    I++E D
Sbjct:    8 KVYVGNLPSDIRANEVESVFKKYGRIKDVDVKNRAESSFAFVEYESSRDAEDAIHGRNNYRYEGHTLKVEMSKGSGSDRGGRRGSGGGGYRGSYNG-----RYDDRNSGSGNRPKAFGT---------------------KSKSTEFRVRVSGLPQSASWQDLKDHMKAAGDVSYADVFRDGTGVCDYLNEDDLKYALKTLDDSELKTYRGDVGRIRLERD 202          
BLAST of Arginine:serine rich splicing factor vs. Planmine SMEST
Match: SMESG000015564.1 (SMESG000015564.1)

HSP 1 Score: 65.855 bits (159), Expect = 5.925e-12
Identity = 49/201 (24.38%), Postives = 89/201 (44.28%), Query Frame = 3
Query:   63 KIYVGNLPPDSTSRDIEKLFSCFGKIIDIDIKNRNGPPFAFIQFXXXXXXXXXXXXXXNYDLDGFSLRVEFPREIKQNKKDYKEYSRFENPVYVPPFDPYDMYLPYNYGPFPRQDRFXXXXXXXXXXXXXXXXXXXXXPRPPKSTDYRVFISNIPPYSNWQDIKDFMRRYGDVSYADVFG--NGTGVCDFVSKEDFRYVLK 659
            ++++G L   ++ RD+E+ F  +G+I DI +K+     + F++FD+ RDA+DAI +  + +L G  + +E  R  +++ + +    RF                                                     P  TDYR+ + N+P   +WQD+KD MR+ G+V +AD        G+ +F ++ D +  LK
Sbjct:    3 RVFIGRLSDRASERDVERFFKGYGRIKDIRLKS----GYGFVEFDNERDADDAIHDLHHKELAGGRVVLEHARSGRRSPRRFDSSRRFAGNA-------------------------------------------------PIRTDYRLLVENLPSRVSWQDLKDLMRKTGEVMFADAHKRRQNEGIVEFKTRADMKAALK 150          
The following BLAST results are available for this feature:
BLAST of Arginine:serine rich splicing factor vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
SRSF12.178e-2248.15serine and arginine rich splicing factor 1 [Source... [more]
SRSF15.244e-2148.15serine and arginine rich splicing factor 1 [Source... [more]
SRSF98.048e-2144.05serine and arginine rich splicing factor 9 [Source... [more]
SRSF18.085e-2148.15serine and arginine rich splicing factor 1 [Source... [more]
AC015813.21.308e-1644.87novel transcript[more]
back to top
BLAST of Arginine:serine rich splicing factor vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
rsp-33.218e-1948.00Probable splicing factor, arginine/serine-rich 3 ... [more]
rsp-33.218e-1948.00Probable splicing factor, arginine/serine-rich 3 ... [more]
rsp-33.218e-1948.00Probable splicing factor, arginine/serine-rich 3 ... [more]
rsp-33.218e-1948.00Probable splicing factor, arginine/serine-rich 3 ... [more]
rsp-33.218e-1948.00Probable splicing factor, arginine/serine-rich 3 ... [more]
back to top
BLAST of Arginine:serine rich splicing factor vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
SF23.823e-3936.52gene:FBgn0283477 transcript:FBtr0083272[more]
SF23.823e-3936.52gene:FBgn0283477 transcript:FBtr0304727[more]
B526.609e-933.82gene:FBgn0004587 transcript:FBtr0082802[more]
B526.947e-933.82gene:FBgn0004587 transcript:FBtr0082801[more]
B526.947e-933.82gene:FBgn0004587 transcript:FBtr0308196[more]
back to top
BLAST of Arginine:serine rich splicing factor vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
srsf1a9.448e-2350.62serine and arginine rich splicing factor 1a [Sourc... [more]
srsf1a9.736e-2350.62serine and arginine rich splicing factor 1a [Sourc... [more]
srsf1a9.736e-2350.62serine and arginine rich splicing factor 1a [Sourc... [more]
srsf1b1.112e-2250.62serine and arginine rich splicing factor 1b [Sourc... [more]
srsf92.108e-1940.24serine and arginine rich splicing factor 9 [Source... [more]
back to top
BLAST of Arginine:serine rich splicing factor vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
tent5a1.495e-2250.62terminal nucleotidyltransferase 5A [Source:Xenbase... [more]
tent5a2.434e-2250.62terminal nucleotidyltransferase 5A [Source:Xenbase... [more]
lhx31.587e-2147.44LIM homeobox 3 [Source:Xenbase;Acc:XB-GENE-490075][more]
grm61.192e-1329.90glutamate receptor, metabotropic 6 [Source:Xenbase... [more]
grm61.201e-1329.90glutamate receptor, metabotropic 6 [Source:Xenbase... [more]
back to top
BLAST of Arginine:serine rich splicing factor vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Srsf93.061e-3336.89serine/arginine-rich splicing factor 9 [Source:MGI... [more]
Srsf13.600e-2148.15serine/arginine-rich splicing factor 1 [Source:MGI... [more]
Srsf13.600e-2148.15serine/arginine-rich splicing factor 1 [Source:MGI... [more]
Srsf15.550e-2148.15serine/arginine-rich splicing factor 1 [Source:MGI... [more]
Srsf15.550e-2148.15serine/arginine-rich splicing factor 1 [Source:MGI... [more]
back to top
BLAST of Arginine:serine rich splicing factor vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q5PPI1|SRSF9_RAT1.206e-3236.84Serine/arginine-rich splicing factor 9 OS=Rattus n... [more]
sp|Q9D0B0|SRSF9_MOUSE2.141e-3236.89Serine/arginine-rich splicing factor 9 OS=Mus musc... [more]
sp|Q5ZML3|SRSF1_CHICK6.436e-2346.32Serine/arginine-rich splicing factor 1 OS=Gallus g... [more]
sp|Q7SXP4|SRS1A_DANRE7.350e-2250.62Serine/arginine-rich splicing factor 1A OS=Danio r... [more]
sp|Q6NYA0|SRS1B_DANRE8.392e-2250.62Serine/arginine-rich splicing factor 1B OS=Danio r... [more]
back to top
BLAST of Arginine:serine rich splicing factor vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A0R3STR23.223e-4241.15Uncharacterized protein OS=Hymenolepis diminuta OX... [more]
A0A0R3TNU73.744e-4241.15Uncharacterized protein OS=Rodentolepis nana OX=10... [more]
A0A3Q4BTH69.153e-4042.36Uncharacterized protein OS=Mola mola OX=94237 PE=4... [more]
A0A4W6D9H01.193e-3942.79Uncharacterized protein OS=Lates calcarifer OX=818... [more]
A0A023FXP31.674e-3942.67Putative alternative splicing factor asf/sf2 rrm s... [more]
back to top
BLAST of Arginine:serine rich splicing factor vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
srsf1b2.769e-2350.62serine and arginine rich splicing factor 1b [Sourc... [more]
srsf1a6.113e-2350.62serine and arginine rich splicing factor 1 [Source... [more]
srsf91.202e-1940.24serine/arginine-rich splicing factor 9 [Source:NCB... [more]
srsf5b7.356e-1328.90serine/arginine-rich splicing factor 5 [Source:NCB... [more]
srsf5b7.356e-1328.90serine/arginine-rich splicing factor 5 [Source:NCB... [more]
back to top
BLAST of Arginine:serine rich splicing factor vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 3
Match NameE-valueIdentityDescription
SRSF51.681e-1428.13serine and arginine rich splicing factor 5 [Source... [more]
ENSPMAT00000010377.11.276e-1142.86pep scaffold:Pmarinus_7.0:GL481287:28:8451:-1 gene... [more]
ENSPMAT00000006373.13.768e-640.22pep scaffold:Pmarinus_7.0:GL478975:164155:166212:-... [more]
back to top
BLAST of Arginine:serine rich splicing factor vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 1
Match NameE-valueIdentityDescription
NPL39.430e-732.91RNA-binding protein; promotes elongation, regulate... [more]
back to top
BLAST of Arginine:serine rich splicing factor vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO446468.209e-3335.24Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO477765.441e-1830.37Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO316209.020e-1423.08Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO324432.519e-1136.76Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO392902.430e-1035.71Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
back to top
BLAST of Arginine:serine rich splicing factor vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
srsf1a6.290e-3939.30serine/arginine-rich splicing factor 1 [Source:NCB... [more]
ENSORLT00000038036.11.269e-3740.08serine/arginine-rich splicing factor 1B [Source:NC... [more]
srsf91.466e-2933.78serine/arginine-rich splicing factor 9 [Source:NCB... [more]
srsf93.179e-2432.87serine/arginine-rich splicing factor 9 [Source:NCB... [more]
ENSORLT00000006699.26.004e-2249.38serine/arginine-rich splicing factor 1B [Source:NC... [more]
back to top
BLAST of Arginine:serine rich splicing factor vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000013050.13.506e-144100.00SMESG000013050.1[more]
SMESG000013050.13.411e-122100.00SMESG000013050.1[more]
SMESG000077573.12.106e-4742.11SMESG000077573.1[more]
SMESG000075205.11.618e-3334.84SMESG000075205.1[more]
SMESG000015564.15.925e-1224.38SMESG000015564.1[more]
back to top
Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30023920 ID=SMED30023920|Name=Arginine:serine rich splicing factor|organism=Schmidtea mediterranea sexual|type=transcript|length=976bp
AATTGGTTATCAATAAAATTTGAATTTAAAATAATCTTTACCCAATCATG
TCAAATGAGCGGAAGATATACGTAGGCAACTTACCTCCGGATTCTACGTC
CAGAGACATAGAAAAGTTATTCAGTTGCTTTGGTAAAATAATAGATATCG
ACATTAAAAACAGAAATGGTCCACCGTTTGCCTTTATTCAATTTGATGAT
AGAAGAGATGCTGAGGATGCTATTAGAGAACGAGATAATTATGACTTAGA
TGGATTTTCCTTGAGAGTCGAGTTTCCAAGAGAGATTAAACAAAATAAAA
AAGACTACAAAGAGTATTCTCGATTCGAAAATCCGGTATACGTACCACCG
TTTGATCCATACGACATGTATTTACCATATAATTATGGACCGTTTCCAAG
GCAAGATAGATTTTCATATTATGGAGATTACAGGCGTAACAATAACTACG
GCAATTATTCATATCAAAACAGAAGACCAAGGCCTCCTAAGAGTACTGAT
TATCGTGTTTTTATTTCAAATATACCGCCGTACTCAAATTGGCAGGATAT
AAAGGATTTCATGAGAAGGTATGGCGACGTAAGCTACGCAGATGTATTTG
GAAACGGAACCGGTGTTTGTGATTTTGTGTCGAAAGAGGATTTTAGATAC
GTGTTGAAATATGTTAACAACGTTAAATTTAAAACAGTTTCGGGTCAAAC
TTCATATATCAAAGTTGAGGAAGATAAAACTACTAAAAGAGGTTATCACA
GAAGATCACCTCGAACGCATAATAAGTCAAGAAGTAGATCACGGTCGAAG
AGTAAATCGAGGAGTAGCAAATCGTCTCATTCAACTACTCGCGATAAAAG
TTCAACCAGTTCAGAGAGGTCAAAGAGCGGTCGAAAACGAATTTCCAAGT
CAAGGTCAAAAGAGAATGATTAATGGTTCTCAATTAAAATTGAATTTGTT
GTAAAACTCTGTTTATTCAATTTAAT
back to top

protein sequence of SMED30023920-orf-1

>SMED30023920-orf-1 ID=SMED30023920-orf-1|Name=SMED30023920-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=292bp
MSNERKIYVGNLPPDSTSRDIEKLFSCFGKIIDIDIKNRNGPPFAFIQFD
DRRDAEDAIRERDNYDLDGFSLRVEFPREIKQNKKDYKEYSRFENPVYVP
PFDPYDMYLPYNYGPFPRQDRFSYYGDYRRNNNYGNYSYQNRRPRPPKST
DYRVFISNIPPYSNWQDIKDFMRRYGDVSYADVFGNGTGVCDFVSKEDFR
YVLKYVNNVKFKTVSGQTSYIKVEEDKTTKRGYHRRSPRTHNKSRSRSRS
KSKSRSSKSSHSTTRDKSSTSSERSKSGRKRISKSRSKEND*
back to top
Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: molecular function
TermDefinition
GO:0003723RNA binding
GO:0003676nucleic acid binding
GO:0000166nucleotide binding
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000419prepharyngeal region
PLANA:0000420parapharyngeal region
PLANA:0000429neoblast
Vocabulary: INTERPRO
TermDefinition
IPR035979RBD_domain_sf
IPR000504RRM_dom
IPR012677Nucleotide-bd_a/b_plait_sf
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000504RNA recognition motif domainSMARTSM00360rrm1_1coord: 153..222
e-value: 3.4E-6
score: 36.6
coord: 6..75
e-value: 6.1E-20
score: 82.3
IPR000504RNA recognition motif domainPFAMPF00076RRM_1coord: 154..212
e-value: 1.4E-8
score: 34.4
coord: 7..72
e-value: 2.4E-17
score: 62.5
IPR000504RNA recognition motif domainPROSITEPS50102RRMcoord: 5..79
score: 17.285
IPR000504RNA recognition motif domainPROSITEPS50102RRMcoord: 152..228
score: 10.582
IPR012677Nucleotide-binding alpha-beta plait domain superfamilyGENE3DG3DSA:3.30.70.330coord: 151..227
e-value: 1.8E-17
score: 65.0
IPR012677Nucleotide-binding alpha-beta plait domain superfamilyGENE3DG3DSA:3.30.70.330coord: 1..87
e-value: 1.2E-23
score: 85.3
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 225..291
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 233..258
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 259..291
NoneNo IPR availablePANTHERPTHR23003RNA RECOGNITION MOTIF RRM DOMAIN CONTAINING PROTEINcoord: 4..272
NoneNo IPR availablePANTHERPTHR23003:SF27NUCLEOLAR PROTEIN 3coord: 4..272
IPR035979RNA-binding domain superfamilySUPERFAMILYSSF54928RNA-binding domain, RBDcoord: 4..213