Dynein light chain

Overview
NameDynein light chain
Smed IDSMED30023876
Length (bp)636
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Dynein light chain (SMED30023876) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Dynein light chain (SMED30023876) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30023876

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 3

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
parapharyngeal regionSMED30023876h1SMcG0016611 SmedASXL_005125SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
pharynxSMED30023876h1SMcG0016611 dd_Smed_v4_17388_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30023876h1SMcG0016611 dd_Smed_v6_17388_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Dynein light chain vs. Ensembl Celegans
Match: dlc-1 (Dynein light chain 1, cytoplasmic [Source:UniProtKB/Swiss-Prot;Acc:Q22799])

HSP 1 Score: 45.4394 bits (106), Expect = 1.391e-6
Identity = 23/77 (29.87%), Postives = 42/77 (54.55%), Query Frame = 1 
Query:  355 MDDTMKTFIIRLARKAQKELATEYEIARFIKQSLDLRWGGLWQVIVSRYPYACYLSHEMASLIHFWLLEHSFCIWKT 585
            M D M+   I  A +A ++   E +IA +IK+  D ++   W  IV R  +  Y++HE    I+F+L + +  ++K+
Sbjct:   13 MSDDMQQDAIDCATQALEKYNIEKDIAAYIKKEFDKKYNPTWHCIVGR-NFGSYVTHETKHFIYFYLGQVAILLFKS 88
[Close]
BLAST of Dynein light chain vs. Ensembl Celegans
Match: dlc-1 (Dynein light chain 1, cytoplasmic [Source:UniProtKB/Swiss-Prot;Acc:Q22799])

HSP 1 Score: 45.4394 bits (106), Expect = 1.391e-6
Identity = 23/77 (29.87%), Postives = 42/77 (54.55%), Query Frame = 1 
Query:  355 MDDTMKTFIIRLARKAQKELATEYEIARFIKQSLDLRWGGLWQVIVSRYPYACYLSHEMASLIHFWLLEHSFCIWKT 585
            M D M+   I  A +A ++   E +IA +IK+  D ++   W  IV R  +  Y++HE    I+F+L + +  ++K+
Sbjct:   13 MSDDMQQDAIDCATQALEKYNIEKDIAAYIKKEFDKKYNPTWHCIVGR-NFGSYVTHETKHFIYFYLGQVAILLFKS 88
[Close]
BLAST of Dynein light chain vs. Ensembl Celegans
Match: dlc-1 (Dynein light chain 1, cytoplasmic [Source:UniProtKB/Swiss-Prot;Acc:Q22799])

HSP 1 Score: 45.4394 bits (106), Expect = 1.391e-6
Identity = 23/77 (29.87%), Postives = 42/77 (54.55%), Query Frame = 1 
Query:  355 MDDTMKTFIIRLARKAQKELATEYEIARFIKQSLDLRWGGLWQVIVSRYPYACYLSHEMASLIHFWLLEHSFCIWKT 585
            M D M+   I  A +A ++   E +IA +IK+  D ++   W  IV R  +  Y++HE    I+F+L + +  ++K+
Sbjct:   13 MSDDMQQDAIDCATQALEKYNIEKDIAAYIKKEFDKKYNPTWHCIVGR-NFGSYVTHETKHFIYFYLGQVAILLFKS 88
[Close]
BLAST of Dynein light chain vs. Ensembl Celegans
Match: dlc-2 (Probable dynein light chain 2, cytoplasmic [Source:UniProtKB/Swiss-Prot;Acc:Q21557])

HSP 1 Score: 45.0542 bits (105), Expect = 1.943e-6
Identity = 23/72 (31.94%), Postives = 41/72 (56.94%), Query Frame = 1 
Query:  346 EPLMDDTMKTFIIRLARKAQKELATEYEIARFIKQSLDLRWGGLWQVIVSRYPYACYLSHEMASLIHFWLLE 561
            E  M+D  +  +I + R+AQ+    + ++A F+K+ LD ++G  W VI  +  +   +S+EM    HF LL+
Sbjct:   10 ETDMEDPQRDMVISVVREAQRLYNIDKDVAAFVKEELDKKFGATWHVICGK-CFGSRVSYEMG---HFILLK 77
[Close]
BLAST of Dynein light chain vs. Ensembl Fly
Match: Cdlc2 (gene:FBgn0026141 transcript:FBtr0077924)

HSP 1 Score: 43.5134 bits (101), Expect = 7.588e-6
Identity = 22/77 (28.57%), Postives = 42/77 (54.55%), Query Frame = 1 
Query:  355 MDDTMKTFIIRLARKAQKELATEYEIARFIKQSLDLRWGGLWQVIVSRYPYACYLSHEMASLIHFWLLEHSFCIWKT 585
            M + M+   +  A +A ++   E +IA FIK+  D ++   W  IV R  +  Y++HE    I+F+L + +  ++K+
Sbjct:   13 MSEEMQQDAVDCATQALEKYNIEKDIAAFIKKEFDKKYNPTWHCIVGR-NFGSYVTHETRHFIYFYLGQVAILLFKS 88
[Close]
BLAST of Dynein light chain vs. Ensembl Fly
Match: Cdlc2 (gene:FBgn0026141 transcript:FBtr0305553)

HSP 1 Score: 43.5134 bits (101), Expect = 7.588e-6
Identity = 22/77 (28.57%), Postives = 42/77 (54.55%), Query Frame = 1 
Query:  355 MDDTMKTFIIRLARKAQKELATEYEIARFIKQSLDLRWGGLWQVIVSRYPYACYLSHEMASLIHFWLLEHSFCIWKT 585
            M + M+   +  A +A ++   E +IA FIK+  D ++   W  IV R  +  Y++HE    I+F+L + +  ++K+
Sbjct:   13 MSEEMQQDAVDCATQALEKYNIEKDIAAFIKKEFDKKYNPTWHCIVGR-NFGSYVTHETRHFIYFYLGQVAILLFKS 88
[Close]
BLAST of Dynein light chain vs. Ensembl Fly
Match: Cdlc2 (gene:FBgn0026141 transcript:FBtr0077925)

HSP 1 Score: 43.5134 bits (101), Expect = 7.588e-6
Identity = 22/77 (28.57%), Postives = 42/77 (54.55%), Query Frame = 1 
Query:  355 MDDTMKTFIIRLARKAQKELATEYEIARFIKQSLDLRWGGLWQVIVSRYPYACYLSHEMASLIHFWLLEHSFCIWKT 585
            M + M+   +  A +A ++   E +IA FIK+  D ++   W  IV R  +  Y++HE    I+F+L + +  ++K+
Sbjct:   13 MSEEMQQDAVDCATQALEKYNIEKDIAAFIKKEFDKKYNPTWHCIVGR-NFGSYVTHETRHFIYFYLGQVAILLFKS 88
[Close]
BLAST of Dynein light chain vs. UniProt/SwissProt
Match: sp|P14202|TEGU_SCHMA (Tegument antigen OS=Schistosoma mansoni OX=6183 GN=A12 PE=2 SV=1)

HSP 1 Score: 64.3142 bits (155), Expect = 1.543e-11
Identity = 44/195 (22.56%), Postives = 84/195 (43.08%), Query Frame = 1 
Query:   49 DFLKTFEEIDGDFSGEISYEEISTYMRRHNFEAEFVDKWLTLFEPNKDGRITIESYCGSLGLIPREHYYGRVVANRKVGSLIPRGNATEDWANVRIIRLEPLMDDTMKTFIIRLARKAQKELATEY---------------EIARFIKQSLDLRWGGLWQVIVSRYPYACYLSHEMASLIHFWLLEHSFCIWKTP 588
            +F++ F EID D +  I  +E+  Y +++  + + +D W+  F+ +KD +I+IE +C   GL              KV  +    +  +   + +  +L P     ++     +++  Q E+  ++               E+A  +K  LD  +G +WQV++    Y    SHE    I F    +    W+TP
Sbjct:   12 EFIRAFLEIDADSNEMIDKQELIKYCQKYRLDMKLIDPWIARFDTDKDNKISIEEFCRGFGL--------------KVSEIRREKDELKKERDGKFPKLPP----NIEIIAATMSKTKQYEICCQFKEYVDNTSRTGNDMREVANKMKSLLDNTYGRVWQVVLLTGSYWMNFSHEPFLSIQFKYNNYVCLAWRTP 188
[Close]
BLAST of Dynein light chain vs. UniProt/SwissProt
Match: sp|Q6FUJ0|DYL1_CANGA (Dynein light chain 1, cytoplasmic OS=Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65) OX=284593 GN=DYN2 PE=3 SV=1)

HSP 1 Score: 57.3806 bits (137), Expect = 5.146e-10
Identity = 27/77 (35.06%), Postives = 45/77 (58.44%), Query Frame = 1 
Query:  355 MDDTMKTFIIRLARKAQKELATEYEIARFIKQSLDLRWGGLWQVIVSRYPYACYLSHEMASLIHFWLLEHSFCIWKT 585
            M D M+  + R+A +A +E   E EIA  IK+ +D R+G  W VIV R  +  Y++HE    ++F++   +  ++KT
Sbjct:   11 MGDEMQQEVFRIAEEAMREHTLEREIASVIKKEMDSRYGHTWHVIVGR-SFGSYVTHEKGKFVYFYVGPLALLVFKT 86
[Close]
BLAST of Dynein light chain vs. UniProt/SwissProt
Match: sp|P32070|SM21_SCHMA (Antigen Sm21.7 OS=Schistosoma mansoni OX=6183 GN=SM21.7 PE=2 SV=1)

HSP 1 Score: 59.3066 bits (142), Expect = 9.517e-10
Identity = 50/180 (27.78%), Postives = 81/180 (45.00%), Query Frame = 1 
Query:   76 DGDFSGEISYEEISTYMRRHNFEAEFVDKWLTLFEPNKDGRITIESYCGSLGL------IPREHYYGRVVANRKVGSLIPRGNATEDWANVRIIRLEPLMDDTMKTFIIRLARKAQKELATEYEIARFI---KQSLDLRWGGLWQVIVSRYPYACYLSHEMASLIHFWLLEHSFCIWKTP 588
            DGD + E S  ++S   R+   + + V++W+ LF+ +KD +IT E +C  LGL      I R H   + V + +  SL P G        V II     M    +  + +L +    E+  + ++ + +   K  L+ R+G +WQV    + Y    SHE    I F         W+TP
Sbjct:   18 DGDETVETS--KLSESCRKEKLDMKQVNEWIALFDVDKDQKITFEEFCRGLGLKQNEMRIERNHI--KTVQSGREQSL-PEG--------VSII--ASTMPKPKQVEVTQLFKDIYNEVKKDPDMNKVVKTFKSELERRYGRVWQVNAVTHSYWASFSHEPFQSIQFQYDNKIILAWRTP 182
[Close]
BLAST of Dynein light chain vs. UniProt/SwissProt
Match: sp|Q6CWX4|DYL1_KLULA (Dynein light chain 1, cytoplasmic OS=Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) OX=284590 GN=DYN2 PE=3 SV=1)

HSP 1 Score: 49.2914 bits (116), Expect = 5.675e-7
Identity = 24/77 (31.17%), Postives = 42/77 (54.55%), Query Frame = 1 
Query:  355 MDDTMKTFIIRLARKAQKELATEYEIARFIKQSLDLRWGGLWQVIVSRYPYACYLSHEMASLIHFWLLEHSFCIWKT 585
            + D ++  I  L+  A      E EIA +IK+ LD+  G  W VIV +  +  Y++HE    ++F++   +F ++KT
Sbjct:   11 ITDELRDEIFELSSNATANYKLEREIAAYIKKQLDVSQGETWHVIVGK-NFGSYVTHEKGYFVYFYIGPLAFLVFKT 86
[Close]
BLAST of Dynein light chain vs. UniProt/SwissProt
Match: sp|Q759T0|DYL1_ASHGO (Dynein light chain 1, cytoplasmic OS=Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) OX=284811 GN=DYN2 PE=3 SV=1)

HSP 1 Score: 48.521 bits (114), Expect = 1.016e-6
Identity = 23/77 (29.87%), Postives = 45/77 (58.44%), Query Frame = 1 
Query:  355 MDDTMKTFIIRLARKAQKELATEYEIARFIKQSLDLRWGGLWQVIVSRYPYACYLSHEMASLIHFWLLEHSFCIWKT 585
            + D ++  I  ++ +A ++   E E+A +IK+ LD + G  W VIV +  +  Y++HE    I+F++   +F ++KT
Sbjct:   12 ITDELRDEIFGISVQAVEQFQLEREVAAYIKKELDSKHGQTWHVIVGK-NFGSYVTHEKGHFIYFYIGPLAFLVFKT 87
[Close]
BLAST of Dynein light chain vs. TrEMBL
Match: A0A267EIW9 (Dynein light chain OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig005207g1 PE=3 SV=1)

HSP 1 Score: 174.096 bits (440), Expect = 1.173e-51
Identity = 83/183 (45.36%), Postives = 117/183 (63.93%), Query Frame = 1 
Query:   46 LDFLKTFEEIDGDFSGEISYEEISTYMRRHNFEAEFVDKWLTLFEPNKDGRITIESYCGSLGLIPREHYYGRVVANRKVGSLIPRGNATEDWANVRIIRLEPLMDDTMKTFIIRLARKAQKELATEYEIARFIKQSLDLRWGGLWQVIVSRYPYACYLSHEMASLIHFWLLEHSFCIWKTPDL 594
            +DFL  FE+ID DFSGEI+ EE+  Y RRHN+E  FV+KWL LF+ +  G ITIE +C +LGL+P++ Y  +V  NR+       G    D   V+II  +P + D+ K  I+R+AR+AQ++   E +IAR +K  LD  +G  W VIV RY Y  + +HE  SL HF++L ++  +WKTPD 
Sbjct:    1 MDFLHAFEDIDSDFSGEITTEELEAYCRRHNYEDRFVEKWLRLFDHDNSGTITIEEFCDTLGLVPKQEYMEKVQTNREKSE----GGGIMD--GVKIITEDPELSDSTKEEIVRIARQAQEQFVNERDIARHMKARLDDMFGRAWHVIVGRYQYGSFCTHETGSLFHFYVLRNAVLVWKTPDF 177
[Close]
BLAST of Dynein light chain vs. TrEMBL
Match: A0A1I8I7N8 (Dynein light chain OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig001380g1 PE=3 SV=1)

HSP 1 Score: 169.474 bits (428), Expect = 7.377e-50
Identity = 78/182 (42.86%), Postives = 118/182 (64.84%), Query Frame = 1 
Query:   46 LDFLKTFEEIDGDFSGEISYEEISTYMRRHNFEAEFVDKWLTLFEPNKDGRITIESYCGSLGLIPREHYYGRVVANRKVGSLIPRGNATEDWANVRIIRLEPLMDDTMKTFIIRLARKAQKELATEYEIARFIKQSLDLRWGGLWQVIVSRYPYACYLSHEMASLIHFWLLEHSFCIWKTPD 591
            +DFL  +E ID DFSGEI+ EE+  Y R+HN+E +FV+KWL LF+ +  G ITIE +C +LGL+P++ Y  +V  NR+  S +  G        V+II  +P M D +K  I++LAR AQ++   E +IAR++K  LD ++   W VIV R+ Y  + +HE+  L HF+++ ++  +WKTPD
Sbjct:    1 MDFLTAYEAIDTDFSGEITSEELEAYCRKHNYEDKFVEKWLRLFDHDNSGTITIEEFCETLGLVPKQEYMEKVQINREKSSGVMDG--------VKIITEDPDMTDELKEEIVQLARTAQEQFENERDIARYMKSELDAKFDRAWHVIVGRFQYGSFCTHEVGRLFHFYVVRNAILVWKTPD 174
[Close]
BLAST of Dynein light chain vs. TrEMBL
Match: A0A1I8IZR3 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 176.022 bits (445), Expect = 3.704e-49
Identity = 84/185 (45.41%), Postives = 119/185 (64.32%), Query Frame = 1 
Query:   40 MSLDFLKTFEEIDGDFSGEISYEEISTYMRRHNFEAEFVDKWLTLFEPNKDGRITIESYCGSLGLIPREHYYGRVVANRKVGSLIPRGNATEDWANVRIIRLEPLMDDTMKTFIIRLARKAQKELATEYEIARFIKQSLDLRWGGLWQVIVSRYPYACYLSHEMASLIHFWLLEHSFCIWKTPDL 594
            +S+DFL  FE+ID DFSGEI+ EE+  Y RRHN+E  FV+KWL LF+ +  G ITIE +C +LGL+P++ Y  +V  NR+       G    D   V+II  +P + D+ K  I+R+AR+AQ++   E +IAR +K  LD  +G  W VIV RY Y  + +HE  SL HF++L ++  +WKTPD 
Sbjct:  281 ISMDFLHAFEDIDSDFSGEITTEELEAYCRRHNYEDRFVEKWLRLFDHDNSGTITIEEFCDTLGLVPKQEYMEKVQTNREKSE----GGGIMD--GVKIITEDPELSDSTKEEIVRIARQAQEQFVNERDIARHMKARLDDMFGRAWHVIVGRYQYGSFCTHETGSLFHFYVLRNAVLVWKTPDF 459
[Close]
BLAST of Dynein light chain vs. TrEMBL
Match: A0A1I8IZM2 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 160.999 bits (406), Expect = 9.114e-46
Identity = 78/174 (44.83%), Postives = 111/174 (63.79%), Query Frame = 1 
Query:   46 LDFLKTFEEIDGDFSGEISYEEISTYMRRHNFEAEFVDKWLTLFEPNKDGRITIESYCGSLGLIPREHYYGRVVANRKVGSLIPRGNATEDWANVRIIRLEPLMDDTMKTFIIRLARKAQKELATEYEIARFIKQSLDLRWGGLWQVIVSRYPYACYLSHEMASLIHFWLLEHS 567
            +DFL  FE+ID DFSGEI+ EE+  Y RRHN+E  FV+KWL LF+ +  G ITIE +C +LGL+P++ Y  +V  NR+       G    D   V+II  +P + D+ K  I+R+AR+AQ++   E +IAR +K  LD  +G  W VIV RY Y  + +HE  SL HF++L ++
Sbjct:    1 MDFLHAFEDIDSDFSGEITTEELEAYCRRHNYEDRFVEKWLRLFDHDNSGTITIEEFCDTLGLVPKQEYMEKVQTNREK----SEGGGIMD--GVKIITEDPELSDSTKEEIVRIARQAQEQFVNERDIARHMKARLDDMFGRAWHVIVGRYQYGSFCTHETGSLFHFYVLRNA 168
[Close]
BLAST of Dynein light chain vs. TrEMBL
Match: A0A267F2R4 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig009106g1 PE=4 SV=1)

HSP 1 Score: 156.377 bits (394), Expect = 1.282e-44
Identity = 78/181 (43.09%), Postives = 107/181 (59.12%), Query Frame = 1 
Query:   49 DFLKTFEEIDGDFSGEISYEEISTYMRRHNFEAEFVDKWLTLFEPNKDGRITIESYCGSLGLIPREHYYGRVVANRKVGSLIPRGNATEDWANVRIIRLEPLMDDTMKTFIIRLARKAQKELATEYEIARFIKQSLDLRWGGLWQVIVSRYPYACYLSHEMASLIHFWLLEHSFCIWKTPD 591
            DFLK FE ID DFSGEI+ EE+  Y +R NF+ +F  KWL LF+ +  G I+IE YC  LGL P E Y  +V   R         +  +D   VRII L+  M + ++  I+RLARKAQ E + E +IA++IK  LD      W VIV R+ Y  Y +HE+  + HF++   +  +W+TPD
Sbjct:    3 DFLKAFESIDTDFSGEITREELEAYCKRQNFDEKFTQKWLNLFDIDNSGTISIEEYCEVLGLKPNEDYIEKVTEQRVQAGAAGADDDRDDMDGVRIIALDKEMPEDLQRLIVRLARKAQDEFSVEKDIAKYIKSELDSLDQRAWHVIVGRFQYGSYCTHEIGCMFHFFVNRFAILVWRTPD 183
[Close]
BLAST of Dynein light chain vs. Ensembl Nematostella
Match: EDO44582 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RVI5])

HSP 1 Score: 47.3654 bits (111), Expect = 1.515e-7
Identity = 24/77 (31.17%), Postives = 43/77 (55.84%), Query Frame = 1 
Query:  355 MDDTMKTFIIRLARKAQKELATEYEIARFIKQSLDLRWGGLWQVIVSRYPYACYLSHEMASLIHFWLLEHSFCIWKT 585
            M + M+T  I  A +A ++   E +IA FIK+  D ++   W  IV R  +  Y++HE    I+F+L + +  ++K+
Sbjct:   13 MAEDMQTDAIECATQALEKFNIEKDIAAFIKKEFDKKYNPTWHCIVGR-NFGSYVTHETKHFIYFYLGQVAILLFKS 88
[Close]
BLAST of Dynein light chain vs. Ensembl Medaka
Match: DYNLL2 (dynein light chain 2, cytoplasmic [Source:NCBI gene;Acc:101174042])

HSP 1 Score: 44.669 bits (104), Expect = 3.540e-6
Identity = 21/77 (27.27%), Postives = 42/77 (54.55%), Query Frame = 1 
Query:  355 MDDTMKTFIIRLARKAQKELATEYEIARFIKQSLDLRWGGLWQVIVSRYPYACYLSHEMASLIHFWLLEHSFCIWKT 585
            M D M+   +  A +A ++   E +IA ++K+  D ++   W  IV R  +  Y++HE    I+F+L + +  ++K+
Sbjct:   13 MSDEMQQDAVDCAMQAMEKYNIEKDIAAYVKKEFDKKYNPTWHCIVGR-NFGSYVTHETKHFIYFYLGQVAILLFKS 88
[Close]
BLAST of Dynein light chain vs. Planmine SMEST
Match: SMESG000078154.1 (SMESG000078154.1)

HSP 1 Score: 426.787 bits (1096), Expect = 4.043e-151
Identity = 201/203 (99.01%), Postives = 201/203 (99.01%), Query Frame = 1 
Query:   22 KYQKKVMSLDFLKTFEEIDGDFSGEISYEEISTYMRRHNFEAEFVDKWLTLFEPNKDGRITIESYCGSLGLIPREHYYGRVVANRKVGSLIPRGNATEDWANVRIIRLEPLMDDTMKTFIIRLARKAQKELATEYEIARFIKQSLDLRWGGLWQVIVSRYPYACYLSHEMASLIHFWLLEHSFCIWKTPDLIEDDIPKAIFLT 630
            K  KKVMSLDFLKTFEEIDGDFSGEISYEEISTYMRRHNFEAEFVDKWLTLFEPNKDGRITIESYCGSLGLIPREHYYGRVVANRKVGSLIPRGNATEDWANVRIIRLEPLMDDTMKTFIIRLARKAQKELATEYEIARFIKQSLDLRWGGLWQVIVSRYPYACYLSHEMASLIHFWLLEHSFCIWKTPDLIEDDIPKAIFLT
Sbjct:  234 KISKKVMSLDFLKTFEEIDGDFSGEISYEEISTYMRRHNFEAEFVDKWLTLFEPNKDGRITIESYCGSLGLIPREHYYGRVVANRKVGSLIPRGNATEDWANVRIIRLEPLMDDTMKTFIIRLARKAQKELATEYEIARFIKQSLDLRWGGLWQVIVSRYPYACYLSHEMASLIHFWLLEHSFCIWKTPDLIEDDIPKAIFLT 436
[Close]
BLAST of Dynein light chain vs. Planmine SMEST
Match: SMESG000008592.1 (SMESG000008592.1)

HSP 1 Score: 139.043 bits (349), Expect = 2.973e-41
Identity = 66/187 (35.29%), Postives = 110/187 (58.82%), Query Frame = 1 
Query:   46 LDFLKTFEEIDGDFSGEISYEEISTYMRRHNFEAEFVDKWLTLFEPNKDGRITIESYCGSLGLIPREHYYGRVVANRKVG----SLIPRGN--ATEDWANVRIIRLEPLMDDTMKTFIIRLARKAQKELATEYEIARFIKQSLDLRWGGLWQVIVSRYPYACYLSHEMASLIHFWLLEHSFCIWKTP 588
            +D +K FEEID D+SGEI+ +E+  YM + ++E  FV+KW+ +F+PN  GRIT+E +C +LGL P E Y  +V   ++       + P  N    E+  +V IIR +P      + F I ++ +A K+   E +IA+ IK+ LD  +  +W V+V+R  +  ++SH+    +H+ +    + IW+TP
Sbjct:    1 MDLIKAFEEIDTDYSGEITKDELINYMIKKDYEMGFVNKWMEIFDPNNTGRITLEEFCDALGLQPNEDYLQQVQTKQEESKPMIQICPPFNEETNEEIIDVEIIREDPDTTAEFRQFAIDVSHQASKDHKLEKDIAKSIKKKLDEEYNKIWHVVVARSTFGSFISHKSGKFMHYKIENFCYLIWQTP 187
[Close]
BLAST of Dynein light chain vs. Planmine SMEST
Match: SMESG000042102.1 (SMESG000042102.1)

HSP 1 Score: 133.65 bits (335), Expect = 3.207e-39
Identity = 63/181 (34.81%), Postives = 105/181 (58.01%), Query Frame = 1 
Query:   46 LDFLKTFEEIDGDFSGEISYEEISTYMRRHNFEAEFVDKWLTLFEPNKDGRITIESYCGSLGLIPREHYYGRVVANRKVGSLIPRGNATEDWANVRIIRLEPLMDDTMKTFIIRLARKAQKELATEYEIARFIKQSLDLRWGGLWQVIVSRYPYACYLSHEMASLIHFWLLEHSFCIWKTP 588
            ++F+K FEEID D SGEI+  E+  YMR  N++  FV+KWL +F+ +  G IT+E +C +LGLIP ++Y  R+   +    L+ R +      ++ +I  +PL D   K F +++ ++A         IA+++K  LD+++G  W V+V    Y  +LSHE  + IH  + + +  +WK P
Sbjct:    1 MEFIKAFEEIDSDNSGEITRGELIRYMRLKNYKPAFVEKWLNIFDADNSGTITLEEFCNTLGLIPNKNYQKRIEKRKSEKELLKRKSLLIPLYDLYVIDEDPLNDPKFKDFCMKICQEATNNENDFISIAKYLKSVLDVKFGRSWNVVVDIDAYGSFLSHEPGAFIHIKVRKFAILLWKVP 181
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BLAST of Dynein light chain vs. Planmine SMEST
Match: SMESG000081226.1 (SMESG000081226.1)

HSP 1 Score: 100.138 bits (248), Expect = 2.508e-26
Identity = 55/187 (29.41%), Postives = 97/187 (51.87%), Query Frame = 1 
Query:   46 LDFLKTFEEIDGDFSGEISYEEISTYMRRHNFEAEFVDKWLTLFEPNKDGRITIESYCGSLGLIPREHYYGRVVANRKVGSLIPRGNATEDWANVRIIRLEPLMDDTMKTFIIRLARKAQKELATEYEIARFIKQSLDLRWGGLWQVIVSRYPYACYLSHEMASLIHFWLLEHSFCIWKTPDLIEDD 606
            ++ LK F EID   +G I+ E++  +  R+     F +KWL +F+   +G+I IE++  ++GL P ++    +  + + G  I          NV II+ +  M   +K  II  +  A      E ++A+ IK  LD  +G +W  +V +  +   +SH   S +H+ + +H+F I+KTP+L  DD
Sbjct:    1 MELLKIFNEIDVGRTGIITEEQLLDFQERNGLN-NFAEKWLNMFDVENEGKIRIENFASTVGLEPEDYVDQTIQNHEESGQFI---------ENVDIIKEDDNMPQNLKDAIINTSIDALNNYEKESQMAKHIKIFLDDNFGKVWHAVVGKSQFGSNISHLPESFMHYKIEDHAFLIFKTPNLQNDD 177
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BLAST of Dynein light chain vs. Planmine SMEST
Match: SMESG000016244.1 (SMESG000016244.1)

HSP 1 Score: 90.1225 bits (222), Expect = 1.912e-22
Identity = 52/180 (28.89%), Postives = 87/180 (48.33%), Query Frame = 1 
Query:   52 FLKTFEEIDGDFSGEISYEEISTYMRRHNFEAEFVDKWLTLFEPNKDGRITIESYCGSLGLIPREHYYGRVVANRKVGSLIPRGNATEDWANVRIIRLEPLMDDTMKTFIIRLARKAQKELATEYEIARFIKQSLDLRWGGLWQVIVSRYPYACYLSHEMASLIHFWLLEHSFCIWKTPD 591
            FL  F++ID   +G IS E++  Y  + N+   F+  +  LF+ N DG IT++ YC +LG+  +      +    ++ +         D  ++ I   +  M +  +  II + R    E   + +IA  +K  LD  +G  W VIV    Y   +S+E + L+HF L   +F IWKT +
Sbjct:    4 FLMAFDDIDRKGTGVISMEDVKNYTSKENYHKNFIQNFFNLFDLNSDGIITLDEYCTALGINDKTKDKILLRRASRLNAAFLNTTGHTDCKDIEIFSSD--MSNGDQAHIIEICRDLLLEYDDQSDIAEELKNKLDEEFGKHWHVIVGIGRYGSNISNEESCLLHFKLSRFAFLIWKTNE 181
The following BLAST results are available for this feature:
BLAST of Dynein light chain vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Dynein light chain vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 4
Match NameE-valueIdentityDescription
dlc-11.391e-629.87Dynein light chain 1, cytoplasmic [Source:UniProt... [more]
dlc-11.391e-629.87Dynein light chain 1, cytoplasmic [Source:UniProt... [more]
dlc-11.391e-629.87Dynein light chain 1, cytoplasmic [Source:UniProt... [more]
dlc-21.943e-631.94Probable dynein light chain 2, cytoplasmic [Sourc... [more]
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BLAST of Dynein light chain vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 3
Match NameE-valueIdentityDescription
Cdlc27.588e-628.57gene:FBgn0026141 transcript:FBtr0077924[more]
Cdlc27.588e-628.57gene:FBgn0026141 transcript:FBtr0305553[more]
Cdlc27.588e-628.57gene:FBgn0026141 transcript:FBtr0077925[more]
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BLAST of Dynein light chain vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Dynein light chain vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Dynein light chain vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Dynein light chain vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|P14202|TEGU_SCHMA1.543e-1122.56Tegument antigen OS=Schistosoma mansoni OX=6183 GN... [more]
sp|Q6FUJ0|DYL1_CANGA5.146e-1035.06Dynein light chain 1, cytoplasmic OS=Candida glabr... [more]
sp|P32070|SM21_SCHMA9.517e-1027.78Antigen Sm21.7 OS=Schistosoma mansoni OX=6183 GN=S... [more]
sp|Q6CWX4|DYL1_KLULA5.675e-731.17Dynein light chain 1, cytoplasmic OS=Kluyveromyces... [more]
sp|Q759T0|DYL1_ASHGO1.016e-629.87Dynein light chain 1, cytoplasmic OS=Ashbya gossyp... [more]
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BLAST of Dynein light chain vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A267EIW91.173e-5145.36Dynein light chain OS=Macrostomum lignano OX=28230... [more]
A0A1I8I7N87.377e-5042.86Dynein light chain OS=Macrostomum lignano OX=28230... [more]
A0A1I8IZR33.704e-4945.41Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A1I8IZM29.114e-4644.83Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A267F2R41.282e-4443.09Uncharacterized protein OS=Macrostomum lignano OX=... [more]
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BLAST of Dynein light chain vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Dynein light chain vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Dynein light chain vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Dynein light chain vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO445821.515e-731.17Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Dynein light chain vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
DYNLL23.540e-627.27dynein light chain 2, cytoplasmic [Source:NCBI gen... [more]
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BLAST of Dynein light chain vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000078154.14.043e-15199.01SMESG000078154.1[more]
SMESG000008592.12.973e-4135.29SMESG000008592.1[more]
SMESG000042102.13.207e-3934.81SMESG000042102.1[more]
SMESG000081226.12.508e-2629.41SMESG000081226.1[more]
SMESG000016244.11.912e-2228.89SMESG000016244.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30023876 ID=SMED30023876|Name=Dynein light chain|organism=Schmidtea mediterranea sexual|type=transcript|length=636bp
CTGTTTAAGTCTTAAAATTAAAAATATCAAAAAAAAGTTATGTCACTGGA
TTTTCTGAAAACCTTTGAAGAAATTGATGGCGACTTTTCTGGAGAAATCA
GTTATGAGGAAATTTCAACATATATGAGAAGGCATAATTTTGAAGCAGAA
TTTGTTGATAAGTGGTTGACATTATTCGAACCAAACAAAGATGGCCGTAT
CACAATAGAATCATATTGCGGATCTCTTGGATTAATTCCTAGAGAGCATT
ATTATGGCAGAGTTGTTGCAAATCGAAAAGTTGGTAGCTTAATACCTCGG
GGAAACGCAACTGAAGATTGGGCTAATGTTAGAATAATTCGACTAGAGCC
ATTGATGGATGACACAATGAAAACGTTTATTATTCGATTAGCGAGAAAAG
CGCAAAAAGAACTGGCGACCGAATATGAAATAGCTCGGTTTATTAAACAA
AGTCTGGATTTAAGATGGGGAGGTCTTTGGCAAGTAATAGTAAGCCGTTA
TCCATATGCATGTTATCTAAGTCACGAAATGGCAAGTTTAATACATTTTT
GGTTGTTAGAGCACAGCTTCTGTATCTGGAAGACCCCGGATTTAATCGAG
GATGACATTCCAAAAGCAATTTTTCTAACATGATCC
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protein sequence of SMED30023876-orf-1

>SMED30023876-orf-1 ID=SMED30023876-orf-1|Name=SMED30023876-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=198bp
MSLDFLKTFEEIDGDFSGEISYEEISTYMRRHNFEAEFVDKWLTLFEPNK
DGRITIESYCGSLGLIPREHYYGRVVANRKVGSLIPRGNATEDWANVRII
RLEPLMDDTMKTFIIRLARKAQKELATEYEIARFIKQSLDLRWGGLWQVI
VSRYPYACYLSHEMASLIHFWLLEHSFCIWKTPDLIEDDIPKAIFLT*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: molecular function
TermDefinition
GO:0003774motor activity
GO:0005509calcium ion binding
Vocabulary: cellular component
TermDefinition
GO:0005737cytoplasm
GO:0005856cytoskeleton
GO:0005874microtubule
GO:0030286dynein complex
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000418head
PLANA:0000420parapharyngeal region
Vocabulary: INTERPRO
TermDefinition
IPR037177DLC_sf
IPR001372Dynein_light_chain_typ-1/2
IPR011992EF-hand-dom_pair
IPR002048EF_hand_dom
Vocabulary: biological process
TermDefinition
GO:0007017microtubule-based process
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001372Dynein light chain, type 1/2SMARTSM01375Dynein_light_2coord: 95..182
e-value: 7.3E-14
score: 62.0
IPR001372Dynein light chain, type 1/2PFAMPF01221Dynein_lightcoord: 106..182
e-value: 1.1E-16
score: 61.0
IPR037177Dynein light chain superfamilyGENE3DG3DSA:3.30.740.10coord: 87..183
e-value: 1.1E-16
score: 62.6
IPR037177Dynein light chain superfamilySUPERFAMILYSSF54648DLCcoord: 105..183
NoneNo IPR availableGENE3DG3DSA:1.10.238.10coord: 2..76
e-value: 3.1E-8
score: 35.3
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 37..69
score: 5.671
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 1..35
score: 9.688
IPR011992EF-hand domain pairSUPERFAMILYSSF47473EF-handcoord: 4..69