Zinc finger MYM-type protein 5
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30023872 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 8
Homology
BLAST of Zinc finger MYM-type protein 5 vs. TrEMBL
Match: A0A3B3C8M6 (Uncharacterized protein OS=Oryzias melastigma OX=30732 PE=4 SV=1) HSP 1 Score: 61.6178 bits (148), Expect = 2.259e-6 Identity = 28/56 (50.00%), Postives = 41/56 (73.21%), Query Frame = 3 Query: 675 YHFPRMKKVENISCFH--FYRRMSNGDKIKRTCLVYFSTKDTVFCFCCQIFSQNKF 836 ++FP K + SC H F+R++SNG++IKRT L+Y KD++FCFCC++FSQ F Sbjct: 177 FNFP--KNQDGRSCHHHYFFRKLSNGERIKRTWLMYSPKKDSLFCFCCKLFSQKSF 230
BLAST of Zinc finger MYM-type protein 5 vs. TrEMBL
Match: A0A3S2PND3 (Uncharacterized protein OS=Oryzias javanicus OX=123683 GN=OJAV_G00051350 PE=4 SV=1) HSP 1 Score: 59.6918 bits (143), Expect = 8.731e-6 Identity = 26/56 (46.43%), Postives = 41/56 (73.21%), Query Frame = 3 Query: 675 YHFPRMKKVENISCFH--FYRRMSNGDKIKRTCLVYFSTKDTVFCFCCQIFSQNKF 836 ++FP K + SC H F+R+++NG++IKRT L+Y +D++FCFCC++FSQ F Sbjct: 176 FNFP--KNQDGRSCHHHYFFRKLANGERIKRTWLMYSPKRDSLFCFCCKLFSQKSF 229 The following BLAST results are available for this feature:
BLAST of Zinc finger MYM-type protein 5 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of Zinc finger MYM-type protein 5 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of Zinc finger MYM-type protein 5 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of Zinc finger MYM-type protein 5 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of Zinc finger MYM-type protein 5 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of Zinc finger MYM-type protein 5 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of Zinc finger MYM-type protein 5 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of Zinc finger MYM-type protein 5 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 2
BLAST of Zinc finger MYM-type protein 5 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of Zinc finger MYM-type protein 5 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of Zinc finger MYM-type protein 5 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of Zinc finger MYM-type protein 5 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of Zinc finger MYM-type protein 5 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of Zinc finger MYM-type protein 5 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 0
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30023872 ID=SMED30023872|Name=Zinc finger MYM-type protein 5|organism=Schmidtea mediterranea sexual|type=transcript|length=1211bpback to top protein sequence of SMED30023872-orf-1 >SMED30023872-orf-1 ID=SMED30023872-orf-1|Name=SMED30023872-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=107bp MARFRKFRYHFPRMKKVENISCFHFYRRMSNGDKIKRTCLVYFSTKDTVFback to top Annotated Terms
The following terms have been associated with this transcript:
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