SMED30023862

Overview
NameSMED30023862
Smed IDSMED30023862
Length (bp)2734
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30023862 (SMED30023862) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30023862 (SMED30023862) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30023862 (SMED30023862) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30023862 (SMED30023862) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30023862

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 31

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
intestinal phagocyteSMED30023862SMESG000009332.1 Contig507newmark_estsPMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
gutSMED30023862SMESG000009332.1 SMESG000009320.1 Contig505newmark_estsPMID:23079596
Forsthoefel et al., 2012		whole organism asexual adult colorimetric in situ hybridization evidence
intestinal phagocyteSMED30023862SMESG000009332.1 SMESG000009320.1 Contig505newmark_estsPMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
secretory cellSMED30023862SMESG000009332.1 SMESG000009320.1 Contig505newmark_estsPMID:23079596
Forsthoefel et al., 2012		whole organism asexual adult colorimetric in situ hybridization evidence
head regionSMED30023862SMESG000009332.1 SMESG000009320.1 Contig505newmark_estsPMID:23079596
Forsthoefel et al., 2012		whole organism asexual adult colorimetric in situ hybridization evidence
parapharyngeal regionSMED30023862SMESG000009332.1 SMESG000009320.1 Contig505newmark_estsPMID:23079596
Forsthoefel et al., 2012		whole organism asexual adult colorimetric in situ hybridization evidence
gutSMED30023862SMESG000009332.1 SMESG000009320.1 Contig505uc_Smed_v2PMID:23079596
Forsthoefel et al., 2012		whole organism asexual adult colorimetric in situ hybridization evidence
intestinal phagocyteSMED30023862SMESG000009332.1 SMESG000009320.1 Contig505uc_Smed_v2PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
secretory cellSMED30023862SMESG000009332.1 SMESG000009320.1 Contig505uc_Smed_v2PMID:23079596
Forsthoefel et al., 2012		whole organism asexual adult colorimetric in situ hybridization evidence
head regionSMED30023862SMESG000009332.1 SMESG000009320.1 Contig505uc_Smed_v2PMID:23079596
Forsthoefel et al., 2012		whole organism asexual adult colorimetric in situ hybridization evidence
parapharyngeal regionSMED30023862SMESG000009332.1 SMESG000009320.1 Contig505uc_Smed_v2PMID:23079596
Forsthoefel et al., 2012		whole organism asexual adult colorimetric in situ hybridization evidence
intestinal phagocyteSMED30023862SMESG000009332.1 SMESG000009320.1 Contig3864newmark_estsPMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30023862SMESG000009332.1 SMESG000009320.1 Contig3864uc_Smed_v2PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
gutSMED30023862SMESG000041448.1 Contig505newmark_estsPMID:23079596
Forsthoefel et al., 2012		whole organism asexual adult colorimetric in situ hybridization evidence
intestinal phagocyteSMED30023862SMESG000041448.1 Contig505newmark_estsPMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
secretory cellSMED30023862SMESG000041448.1 Contig505newmark_estsPMID:23079596
Forsthoefel et al., 2012		whole organism asexual adult colorimetric in situ hybridization evidence
head regionSMED30023862SMESG000041448.1 Contig505newmark_estsPMID:23079596
Forsthoefel et al., 2012		whole organism asexual adult colorimetric in situ hybridization evidence
parapharyngeal regionSMED30023862SMESG000041448.1 Contig505newmark_estsPMID:23079596
Forsthoefel et al., 2012		whole organism asexual adult colorimetric in situ hybridization evidence
gutSMED30023862SMESG000041448.1 Contig505uc_Smed_v2PMID:23079596
Forsthoefel et al., 2012		whole organism asexual adult colorimetric in situ hybridization evidence
intestinal phagocyteSMED30023862SMESG000041448.1 Contig505uc_Smed_v2PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
secretory cellSMED30023862SMESG000041448.1 Contig505uc_Smed_v2PMID:23079596
Forsthoefel et al., 2012		whole organism asexual adult colorimetric in situ hybridization evidence
head regionSMED30023862SMESG000041448.1 Contig505uc_Smed_v2PMID:23079596
Forsthoefel et al., 2012		whole organism asexual adult colorimetric in situ hybridization evidence
parapharyngeal regionSMED30023862SMESG000041448.1 Contig505uc_Smed_v2PMID:23079596
Forsthoefel et al., 2012		whole organism asexual adult colorimetric in situ hybridization evidence
intestinal phagocyteSMED30023862SMESG000052432.1 Contig507newmark_estsPMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30023862SMESG000058668.1 Contig3864newmark_estsPMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30023862SMESG000058668.1 Contig3864uc_Smed_v2PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
X2 cellSMED30023862SMESG000044854.1 SmedASXL_071805SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
nervous systemSMED30023862SMESG000009332.1 SMESG000009320.1 dd_Smed_v4_1555_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30023862SMESG000009332.1 SMESG000009320.1 Contig11338newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30023862SMESG000009332.1 SMESG000009320.1 Contig11338uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
epidermisSMED30023862SMESG000009332.1 SMESG000009320.1 dd_Smed_v4_1555_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
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BLAST of SMED30023862 vs. Planmine SMEST
Match: SMESG000009320.1 (SMESG000009320.1)

HSP 1 Score: 577.4 bits (1487), Expect = 0.000e+0
Identity = 279/282 (98.94%), Postives = 280/282 (99.29%), Query Frame = 3 
Query:   45 MDDEDDKINICSELWNDLEPYSPCIIKFSSPITTGFRFRYQSEIEKKSYSLLKAEKNSSPTLEITTTECYTDLVVELVTADGEPHFNMLYEVPKDKMIEPLVRNGVYNVVLKDPGSYEIKLDLGIRKMKNKEAMVRIKELYPKMKDSEAKSFFNKNKDAVRLKVSCYNNEIPACHICSDVIKSQISKENKMIKIKHFAPKVVADLGEELILVLEKKITTDDFKATLTLNAESYPLKPIEQDGQTFVFNLPRVNCLNSTNGKFTMECGENSDSKPITFIPIQT 890
            MDDEDDKINICSELWNDLEPYSPCIIKFSSPITTGFRFRYQSEIEKKSYSLLKAEKNSSPTLEITTTECYTDLVVELVTADGEPHFNMLYEVPKDKMIEPLVRNGVYNVVLKDPG YEIKLDLGIRKMKNKEAMVRIKELYPKMKDSEAKSFFNKNKDAVRLKVSCYNNEIPACHICSDVIKSQISKENKMIKIKHFAPKVVADLGEELILVLEKKITTDDFKATLTLNAESYPLKPIEQDGQTFVFNLPRVNCLNSTNGKFTMECGENSDSKPI F+PIQT
Sbjct:    1 MDDEDDKINICSELWNDLEPYSPCIIKFSSPITTGFRFRYQSEIEKKSYSLLKAEKNSSPTLEITTTECYTDLVVELVTADGEPHFNMLYEVPKDKMIEPLVRNGVYNVVLKDPGFYEIKLDLGIRKMKNKEAMVRIKELYPKMKDSEAKSFFNKNKDAVRLKVSCYNNEIPACHICSDVIKSQISKENKMIKIKHFAPKVVADLGEELILVLEKKITTDDFKATLTLNAESYPLKPIEQDGQTFVFNLPRVNCLNSTNGKFTMECGENSDSKPIKFLPIQT 282
[Close]
BLAST of SMED30023862 vs. Planmine SMEST
Match: SMESG000009320.1 (SMESG000009320.1)

HSP 1 Score: 577.4 bits (1487), Expect = 0.000e+0
Identity = 279/282 (98.94%), Postives = 280/282 (99.29%), Query Frame = 3 
Query:   45 MDDEDDKINICSELWNDLEPYSPCIIKFSSPITTGFRFRYQSEIEKKSYSLLKAEKNSSPTLEITTTECYTDLVVELVTADGEPHFNMLYEVPKDKMIEPLVRNGVYNVVLKDPGSYEIKLDLGIRKMKNKEAMVRIKELYPKMKDSEAKSFFNKNKDAVRLKVSCYNNEIPACHICSDVIKSQISKENKMIKIKHFAPKVVADLGEELILVLEKKITTDDFKATLTLNAESYPLKPIEQDGQTFVFNLPRVNCLNSTNGKFTMECGENSDSKPITFIPIQT 890
            MDDEDDKINICSELWNDLEPYSPCIIKFSSPITTGFRFRYQSEIEKKSYSLLKAEKNSSPTLEITTTECYTDLVVELVTADGEPHFNMLYEVPKDKMIEPLVRNGVYNVVLKDPG YEIKLDLGIRKMKNKEAMVRIKELYPKMKDSEAKSFFNKNKDAVRLKVSCYNNEIPACHICSDVIKSQISKENKMIKIKHFAPKVVADLGEELILVLEKKITTDDFKATLTLNAESYPLKPIEQDGQTFVFNLPRVNCLNSTNGKFTMECGENSDSKPI F+PIQT
Sbjct:    1 MDDEDDKINICSELWNDLEPYSPCIIKFSSPITTGFRFRYQSEIEKKSYSLLKAEKNSSPTLEITTTECYTDLVVELVTADGEPHFNMLYEVPKDKMIEPLVRNGVYNVVLKDPGFYEIKLDLGIRKMKNKEAMVRIKELYPKMKDSEAKSFFNKNKDAVRLKVSCYNNEIPACHICSDVIKSQISKENKMIKIKHFAPKVVADLGEELILVLEKKITTDDFKATLTLNAESYPLKPIEQDGQTFVFNLPRVNCLNSTNGKFTMECGENSDSKPIKFLPIQT 282
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BLAST of SMED30023862 vs. Planmine SMEST
Match: SMESG000009332.1 (SMESG000009332.1)

HSP 1 Score: 407.912 bits (1047), Expect = 8.809e-134
Identity = 206/259 (79.54%), Postives = 222/259 (85.71%), Query Frame = 3 
Query:  114 CIIKFSSPITTGFRFRYQSEIEKKSYSLLKAEKNSSPTLEITTTECYTDLVVELVTADGEPHFNMLYEVPKDKMIEPLVRNGVYNVVLKDPGSYEIKLDLGIRKMKNKEAMVRIKELYPKMKDSEAKSFFNKNKDAVRLKVSCYNNEIPACHICSDVIKSQISKENKMIKIKHFAPKVVADLGEELILVLEKKITTDDFKATLTLNAESYPLKPIEQDGQTFVFNLPRVNCLNSTNGKFTMECGENSDSKPITFIPIQT 890
            C I+F     TG RFRY+SEI KK YSLLK+  N S  L+I TT+C T+LV+ELVT DG+ HF+ LYEV KD     L+R G +   LK  G + IKLDLGIRKMKNKEAMV+IKELYPKMKDSEAKSFFNKNKDAVRLKVSCYNNEIPACHICSDVIKSQ S+ENKMIKIKHFAPKVVADLGEELILVLEKKITTDDFKATLTLNAESYPLK IEQDGQTFVFNLPRVNCLNSTNGKFTM+CGENSDSKPI F+PIQT
Sbjct:   11 CTIEFGILPQTGNRFRYESEINKKCYSLLKSGNNKSLDLKINTTKCDTNLVIELVTDDGKKHFHNLYEVQKD----GLIRYGCFQKTLKHSGFHLIKLDLGIRKMKNKEAMVQIKELYPKMKDSEAKSFFNKNKDAVRLKVSCYNNEIPACHICSDVIKSQTSRENKMIKIKHFAPKVVADLGEELILVLEKKITTDDFKATLTLNAESYPLKSIEQDGQTFVFNLPRVNCLNSTNGKFTMKCGENSDSKPIKFLPIQT 265

HSP 2 Score: 298.901 bits (764), Expect = 3.548e-92
Identity = 167/171 (97.66%), Postives = 168/171 (98.25%), Query Frame = 1 
Query: 1435 VTQESRKRKEVSVKEDSPLKRINNYDPQDNQSLSVNLMEYITDDRSRNDSDPLICSVTQSDFPFQDEKDIFSIXXXXXXXXXXXXXXXXXXXXFETLYTPEYQHNEGMDWSEHSVGNEIINLREIIMPKYLGSHADYQSNVSLDEIKEPFNDIEGSIDEMNLSKPIFCVRK 1947
            +  ESRKRKEVSVKEDSPLKRINNYDPQDNQSLSVNLMEYITDDRSRNDSDPLICSVTQSDFPFQDEKDIFSIDNTKMDNDNDLDNNIHNDLNFETLYTPEYQHNEGMDWSEHSVGNEIINLREIIMPKYLGSHADYQSNV LDEIKEPFNDIEGSIDEMNLSKPIFCVRK
Sbjct:  263 IQTESRKRKEVSVKEDSPLKRINNYDPQDNQSLSVNLMEYITDDRSRNDSDPLICSVTQSDFPFQDEKDIFSIDNTKMDNDNDLDNNIHNDLNFETLYTPEYQHNEGMDWSEHSVGNEIINLREIIMPKYLGSHADYQSNVPLDEIKEPFNDIEGSIDEMNLSKPIFCVRK 433
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BLAST of SMED30023862 vs. Planmine SMEST
Match: SMESG000030673.1 (SMESG000030673.1)

HSP 1 Score: 46.9802 bits (110), Expect = 1.970e-6
Identity = 25/49 (51.02%), Postives = 35/49 (71.43%), Query Frame = 2 
Query: 1961 RKDI-KEYQRITCIKEE*TKSER*DYIEKCQDFRIAPGKLEYKYNVTEK 2104
            RK++ + YQRI+CI+EE +KS + DY E  QDFRI   + E +YNV +K
Sbjct:   18 RKEVPRRYQRISCIEEEESKSRKQDYTENNQDFRITHSETEERYNVPQK 66
[Close]
BLAST of SMED30023862 vs. Planmine SMEST
Match: SMESG000008586.1 (SMESG000008586.1)

HSP 1 Score: 45.8246 bits (107), Expect = 6.349e-6
Identity = 26/59 (44.07%), Postives = 37/59 (62.71%), Query Frame = 2 
Query: 1931 FFAFESSSPSRKDI-KEYQRITCIKEE*TKSER*DYIEKCQDFRIAPGKLEYKYNVTEK 2104
            ++ F+     RK+  + Y+RI CI+EE +K  + DY E  QDFRI   K+E +YNV EK
Sbjct:    5 WYGFQDCFSLRKEAPRRYRRIPCIEEEDSKFRKQDYTENNQDFRITHSKIEERYNVPEK 63
The following BLAST results are available for this feature:
BLAST of SMED30023862 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023862 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023862 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023862 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023862 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023862 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023862 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023862 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023862 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023862 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023862 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023862 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023862 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023862 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000009320.10.000e+098.94SMESG000009320.1[more]
SMESG000009320.10.000e+098.94SMESG000009320.1[more]
SMESG000009332.18.809e-13479.54SMESG000009332.1[more]
SMESG000030673.11.970e-651.02SMESG000030673.1[more]
SMESG000008586.16.349e-644.07SMESG000008586.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30023862 ID=SMED30023862|Name=SMED30023862|organism=Schmidtea mediterranea sexual|type=transcript|length=2734bp
TCAAATAATTGAATATCCGATAGTGCGAGTAATTCTCGGTACAAATGGAT
GACGAGGACGACAAAATTAATATTTGTAGCGAACTTTGGAATGATCTTGA
ACCGTATTCTCCTTGTATTATAAAATTCAGTTCACCAATAACGACAGGAT
TTAGATTTCGGTACCAATCAGAAATCGAGAAAAAGTCCTATTCATTATTA
AAAGCTGAGAAAAATTCGTCCCCAACTCTAGAAATTACCACTACAGAGTG
TTATACAGATCTTGTTGTTGAATTGGTAACAGCTGATGGAGAACCGCATT
TCAATATGTTATATGAAGTTCCAAAGGACAAAATGATTGAGCCTCTGGTT
CGTAACGGAGTATATAATGTGGTATTAAAAGATCCTGGATCTTATGAAAT
CAAATTAGATCTGGGTATTCGTAAAATGAAGAACAAAGAAGCGATGGTTC
GAATTAAAGAATTATATCCGAAAATGAAGGATTCAGAGGCTAAATCATTT
TTCAATAAAAACAAAGACGCCGTACGTTTAAAAGTATCCTGCTATAATAA
TGAGATTCCAGCATGTCATATTTGTTCTGATGTAATCAAATCGCAAATTT
CTAAAGAAAATAAAATGATAAAAATCAAACATTTCGCACCAAAAGTAGTC
GCAGACTTAGGTGAAGAACTCATACTTGTATTGGAAAAGAAAATCACTAC
TGATGATTTTAAAGCCACACTAACACTAAATGCTGAAAGCTATCCATTAA
AGCCAATAGAACAAGATGGACAAACTTTTGTCTTCAATTTACCTCGCGTC
AATTGTCTTAATTCAACAAATGGAAAGTTTACTATGGAATGTGGAGAGAA
TTCTGATTCTAAACCAATAACATTTATACCTATTCAAACCGGTTATTTAT
TTTCAAATGATATTAAATTTATTCAAATTTCACAGACCGATATAAGTTTA
GCTAAAATTATAAAAAATATCCTAAATGTAGAAAGTATAAAATTGTCAAA
CAAATTAACTATTTATTGAATTTAATTAATTGGGAAAGTTTAAATCAAGC
GGCACATTCTAGGAGGAAAAATCTTTTAGTCTGCTTCTTTTGTAAATTAT
TTCTTTCAGTTGCAACACAAATATGATTTCATATTTTATCAATTGTTTAA
ATAATATCAGAAAATGGGAGATTTAAAATTGAAATTATTAATATAATAAA
TATTTTTTTTAGAAGTTTACTTTTAGAGTTATATAACACGATGTCACAAG
CAGGATTTGACCATTTTTTCCAAAGAATAAATAAATTCTTAGAAACAAAG
AAGTATTTTTTATTTGCAAGCGACTACAAAGTTCGTGATTCGTGAAAATC
GTTGTAAACATACGCTAGGATGAAGGTCATATAATTCAATACCAGAATGC
AAACGGTTATAAATAAATGAAAGTTTAATTTTTAGTCACACAAGAATCAA
GAAAAAGAAAAGAGGTTTCTGTGAAAGAGGATTCACCTCTAAAAAGAATA
AATAATTATGATCCCCAGGATAATCAATCATTGAGCGTGAATTTAATGGA
ATATATCACTGATGATAGATCTAGAAACGATAGCGATCCATTAATATGTT
CAGTAACCCAAAGTGATTTTCCATTTCAGGACGAGAAAGATATATTCTCT
ATAGATAACACTAAAATGGATAATGACAATGATTTAGATAATAATATTCA
CAATGATTTGAATTTTGAAACACTTTATACTCCTGAATATCAGCATAATG
AAGGCATGGACTGGTCTGAACATTCTGTGGGGAATGAAATAATTAATCTC
AGAGAAATAATAATGCCCAAATATCTAGGATCTCATGCTGATTATCAGTC
AAATGTGTCACTTGATGAAATCAAAGAACCTTTTAATGACATTGAAGGAT
CTATTGATGAGATGAACTTGAGCAAACCAATTTTTTGCGTTCGAAAGTTC
TTCTCCATCACGGAAAGATATCAAAGAATATCAAAGAATAACGTGCATAA
AAGAAGAATAAACAAAATCTGAAAGATGAGATTATATCGAAAAATGTCAA
GATTTCAGGATAGCTCCAGGCAAACTAGAATATAAATACAATGTCACTGA
GAAATGTGTTGATATTCTTATTTTCGGTTGAAGTTACAAGACTTAATTTC
CATCATAAGTTTTATTTTGTATAATATCATACAAGCGCCAACAATCAATT
AATAATTCACCAAAACTTGTAATATAAAATTTTTTTTAGAAATAATCTAA
ATTGATACTTTTCTTGAAATACCTGTACACATAGGAGAGTCATACATCAT
TGGTTAGGAATCCCTGCTTTAAAAATTATCTATTCATTTGTTATTTTGTC
TTTTTTGTGGGCAGTTTCACTCTTGGCTGGAATTATTATTATTTCCTCCA
ACTTCCGGATTTTATGGAATAATTGACGGAGTTGGATTTTCTAGAGATTT
TAGGTGTCTTTATGACTTTTAGAATAAGGGTTAATACAAGAAATCTTGGT
TTTTCTGTCAATTTTTAGTTATTTTACGCGAGCACTAAAATATTGCCATA
TTGTATTCAATTGTTTGACTAATCATTCAATCAAATAAAGGAGCGTTTCA
AAGAAGTTAATGGTTTTGGTTAAAAACAGAGTAAAACTTTTTTTTATATC
AGTCGAATTTTTTCAAAAAAACTTTTGTACGATTGATTACTGTATGAGTT
TTTAAATTAAAATAAAATGTCGAGTTATTTTATG
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protein sequence of SMED30023862-orf-1

>SMED30023862-orf-1 ID=SMED30023862-orf-1|Name=SMED30023862-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=159bp
MEYITDDRSRNDSDPLICSVTQSDFPFQDEKDIFSIDNTKMDNDNDLDNN
IHNDLNFETLYTPEYQHNEGMDWSEHSVGNEIINLREIIMPKYLGSHADY
QSNVSLDEIKEPFNDIEGSIDEMNLSKPIFCVRKFFSITERYQRISKNNV
HKRRINKI*
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protein sequence of SMED30023862-orf-2

>SMED30023862-orf-2 ID=SMED30023862-orf-2|Name=SMED30023862-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=325bp
MDDEDDKINICSELWNDLEPYSPCIIKFSSPITTGFRFRYQSEIEKKSYS
LLKAEKNSSPTLEITTTECYTDLVVELVTADGEPHFNMLYEVPKDKMIEP
LVRNGVYNVVLKDPGSYEIKLDLGIRKMKNKEAMVRIKELYPKMKDSEAK
SFFNKNKDAVRLKVSCYNNEIPACHICSDVIKSQISKENKMIKIKHFAPK
VVADLGEELILVLEKKITTDDFKATLTLNAESYPLKPIEQDGQTFVFNLP
RVNCLNSTNGKFTMECGENSDSKPITFIPIQTGYLFSNDIKFIQISQTDI
SLAKIIKNILNVESIKLSNKLTIY*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000026gut
PLANA:0000034epidermis
PLANA:0000070intestinal phagocyte
PLANA:0000099neuron
PLANA:0000105secretory cell
PLANA:0000418head
PLANA:0000420parapharyngeal region
PLANA:0002111X2 cell
Vocabulary: INTERPRO
TermDefinition
IPR011539RHD_DNA_bind_dom
IPR008967p53-like_TF_DNA-bd
Vocabulary: molecular function
TermDefinition
GO:0003700transcription factor activity, sequence-specific DNA binding
GO:0003677DNA binding
Vocabulary: biological process
TermDefinition
GO:0006355regulation of transcription, DNA-templated