Calpain 5a
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30023857 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 9
Homology
BLAST of Calpain 5a vs. Ensembl Human
Match: CAPN5 (calpain 5 [Source:HGNC Symbol;Acc:HGNC:1482]) HSP 1 Score: 336.265 bits (861), Expect = 1.539e-104 Identity = 204/622 (32.80%), Postives = 324/622 (52.09%), Query Frame = 2
Query: 68 FNNQNYVSMRQEHQKNLTLFCDDTFGADDKSISPSGKQEILSSIEWKRPSELCINPCLFIAGSSVHDVCQGSLGNCWLVSALAVLAQHPECLKKVIPNVDDQEWHGNRPHSYTGMFRICLWRYGEWLEVVIDDRLPSRNGKLVFVHSKVKNEFWSALIEKAYAKVCGSYESLVGGLTCESMVDFTGGIPEQIFDLKK--YSKSDTEMENLFKLLHFDWKDGSLMCATI-ASRSDEMEEKLANGLITTHAYSITGVCKLRIKSTPMYEHYQKNHLMCIRLRNPWGQKEWNGSLSDASEEWHSISHKDKIAMGWKVENDGEFWMLIDDVLVNFNEITIIRPIKTGIFKYNSNWFGSMCKGYWTGVAGDRQSALA-------EIGQGGQFKIEINNPKGDIFLC------HLLQKCARGSEAALGILIIAVERNRDFMVHTPKFCNVKLEYRLGGSRSICLRYRLTCGTYIVKPFLTVASPSLKFPIYYLLRCYTTQKNNGTFLPSEMPILERNCICKSN-CTIIICRLHXXXXXXXXXXEELDVLNIFCEIDCENQSLRTTVNCNTSGLFWDETFLLHRSKMDRPIIIKIFHRGRFFNTLIGYSCCSYDSRHLNSPEVIETLNM 1882
+ +QNY ++R++ ++ LF D F A D S+ G ++ WKRP +C +P LF+ G S HD+ QG +GNCW V+A + LA +KVIP+ +QEW +P++Y G+F WR+GEW++VVIDDRLP+ N +L++ HS +NEFW AL+EKAYAK+ G Y++L GG T +++VDFTGG+ E I DL + ++ +T+ LF+ + G L+ A+I A + +ME +LA GL+ HAY++T V K+R+ + ++ L IRLRNPWG++EWNG SD SEEW +S ++ MG V++DGEFWM +DV F +I R I T + W + G WT RQ+ Q Q+ E+ P+ ++ +C ++ +G A+G I VE NR + +H+ + Y SRS+ LR G Y++ P + +F LLR +T +N E+ + E C S+ C + + N + I CE +R+ V TS ++ + +R K+ +PI +++++ + +G D +L + + TL++
Sbjct: 8 YEDQNYSALRRDCRRRKVLFEDPLFPATDDSLYYKGTPG--PAVRWKRPKGICEDPRLFVDGISSHDLHQGQVGNCWFVAACSSLASRESLWQKVIPDWKEQEWDPEKPNAYAGIFHFHFWRFGEWVDVVIDDRLPTVNNQLIYCHSNSRNEFWCALVEKAYAKLAGCYQALDGGNTADALVDFTGGVSEPI-DLTEGDFANDETKRNQLFERMLKVHSRGGLISASIKAVTAADMEARLACGLVKGHAYAVTDVRKVRLGHG-LLAFFKSEKLDMIRLRNPWGEREWNGPWSDTSEEWQKVSKSEREKMGVTVQDDGEFWMTFEDVCRYFTDIIKCRVINTSHLSIHKTWEEARLHGAWTLHEDPRQNRGGGCINHKDTFFQNPQYIFEVKKPEDEVLICIQQRPKRSTRREGKGENLAIGFDIYKVEENRQYRMHSLQHKAASSIYI--NSRSVFLRTDQPEGRYVIIPTTFEPGHTGEF----LLRVFTDVPSNC----RELRLDEPPHTCWSSLCGYPQLVTQVHVLGAAGLKDSPTGANSYVIIKCEGDKVRSAVQKGTSTPEYNVKGIFYRKKLSQPITVQVWNHRVLKDEFLGQVHLKADPDNL---QALHTLHL 612
BLAST of Calpain 5a vs. Ensembl Human
Match: CAPN5 (calpain 5 [Source:HGNC Symbol;Acc:HGNC:1482]) HSP 1 Score: 336.265 bits (861), Expect = 1.539e-104 Identity = 204/622 (32.80%), Postives = 324/622 (52.09%), Query Frame = 2
Query: 68 FNNQNYVSMRQEHQKNLTLFCDDTFGADDKSISPSGKQEILSSIEWKRPSELCINPCLFIAGSSVHDVCQGSLGNCWLVSALAVLAQHPECLKKVIPNVDDQEWHGNRPHSYTGMFRICLWRYGEWLEVVIDDRLPSRNGKLVFVHSKVKNEFWSALIEKAYAKVCGSYESLVGGLTCESMVDFTGGIPEQIFDLKK--YSKSDTEMENLFKLLHFDWKDGSLMCATI-ASRSDEMEEKLANGLITTHAYSITGVCKLRIKSTPMYEHYQKNHLMCIRLRNPWGQKEWNGSLSDASEEWHSISHKDKIAMGWKVENDGEFWMLIDDVLVNFNEITIIRPIKTGIFKYNSNWFGSMCKGYWTGVAGDRQSALA-------EIGQGGQFKIEINNPKGDIFLC------HLLQKCARGSEAALGILIIAVERNRDFMVHTPKFCNVKLEYRLGGSRSICLRYRLTCGTYIVKPFLTVASPSLKFPIYYLLRCYTTQKNNGTFLPSEMPILERNCICKSN-CTIIICRLHXXXXXXXXXXEELDVLNIFCEIDCENQSLRTTVNCNTSGLFWDETFLLHRSKMDRPIIIKIFHRGRFFNTLIGYSCCSYDSRHLNSPEVIETLNM 1882
+ +QNY ++R++ ++ LF D F A D S+ G ++ WKRP +C +P LF+ G S HD+ QG +GNCW V+A + LA +KVIP+ +QEW +P++Y G+F WR+GEW++VVIDDRLP+ N +L++ HS +NEFW AL+EKAYAK+ G Y++L GG T +++VDFTGG+ E I DL + ++ +T+ LF+ + G L+ A+I A + +ME +LA GL+ HAY++T V K+R+ + ++ L IRLRNPWG++EWNG SD SEEW +S ++ MG V++DGEFWM +DV F +I R I T + W + G WT RQ+ Q Q+ E+ P+ ++ +C ++ +G A+G I VE NR + +H+ + Y SRS+ LR G Y++ P + +F LLR +T +N E+ + E C S+ C + + N + I CE +R+ V TS ++ + +R K+ +PI +++++ + +G D +L + + TL++
Sbjct: 8 YEDQNYSALRRDCRRRKVLFEDPLFPATDDSLYYKGTPG--PAVRWKRPKGICEDPRLFVDGISSHDLHQGQVGNCWFVAACSSLASRESLWQKVIPDWKEQEWDPEKPNAYAGIFHFHFWRFGEWVDVVIDDRLPTVNNQLIYCHSNSRNEFWCALVEKAYAKLAGCYQALDGGNTADALVDFTGGVSEPI-DLTEGDFANDETKRNQLFERMLKVHSRGGLISASIKAVTAADMEARLACGLVKGHAYAVTDVRKVRLGHG-LLAFFKSEKLDMIRLRNPWGEREWNGPWSDTSEEWQKVSKSEREKMGVTVQDDGEFWMTFEDVCRYFTDIIKCRVINTSHLSIHKTWEEARLHGAWTLHEDPRQNRGGGCINHKDTFFQNPQYIFEVKKPEDEVLICIQQRPKRSTRREGKGENLAIGFDIYKVEENRQYRMHSLQHKAASSIYI--NSRSVFLRTDQPEGRYVIIPTTFEPGHTGEF----LLRVFTDVPSNC----RELRLDEPPHTCWSSLCGYPQLVTQVHVLGAAGLKDSPTGANSYVIIKCEGDKVRSAVQKGTSTPEYNVKGIFYRKKLSQPITVQVWNHRVLKDEFLGQVHLKADPDNL---QALHTLHL 612
BLAST of Calpain 5a vs. Ensembl Human
Match: CAPN5 (calpain 5 [Source:HGNC Symbol;Acc:HGNC:1482]) HSP 1 Score: 336.265 bits (861), Expect = 1.539e-104 Identity = 204/622 (32.80%), Postives = 324/622 (52.09%), Query Frame = 2
Query: 68 FNNQNYVSMRQEHQKNLTLFCDDTFGADDKSISPSGKQEILSSIEWKRPSELCINPCLFIAGSSVHDVCQGSLGNCWLVSALAVLAQHPECLKKVIPNVDDQEWHGNRPHSYTGMFRICLWRYGEWLEVVIDDRLPSRNGKLVFVHSKVKNEFWSALIEKAYAKVCGSYESLVGGLTCESMVDFTGGIPEQIFDLKK--YSKSDTEMENLFKLLHFDWKDGSLMCATI-ASRSDEMEEKLANGLITTHAYSITGVCKLRIKSTPMYEHYQKNHLMCIRLRNPWGQKEWNGSLSDASEEWHSISHKDKIAMGWKVENDGEFWMLIDDVLVNFNEITIIRPIKTGIFKYNSNWFGSMCKGYWTGVAGDRQSALA-------EIGQGGQFKIEINNPKGDIFLC------HLLQKCARGSEAALGILIIAVERNRDFMVHTPKFCNVKLEYRLGGSRSICLRYRLTCGTYIVKPFLTVASPSLKFPIYYLLRCYTTQKNNGTFLPSEMPILERNCICKSN-CTIIICRLHXXXXXXXXXXEELDVLNIFCEIDCENQSLRTTVNCNTSGLFWDETFLLHRSKMDRPIIIKIFHRGRFFNTLIGYSCCSYDSRHLNSPEVIETLNM 1882
+ +QNY ++R++ ++ LF D F A D S+ G ++ WKRP +C +P LF+ G S HD+ QG +GNCW V+A + LA +KVIP+ +QEW +P++Y G+F WR+GEW++VVIDDRLP+ N +L++ HS +NEFW AL+EKAYAK+ G Y++L GG T +++VDFTGG+ E I DL + ++ +T+ LF+ + G L+ A+I A + +ME +LA GL+ HAY++T V K+R+ + ++ L IRLRNPWG++EWNG SD SEEW +S ++ MG V++DGEFWM +DV F +I R I T + W + G WT RQ+ Q Q+ E+ P+ ++ +C ++ +G A+G I VE NR + +H+ + Y SRS+ LR G Y++ P + +F LLR +T +N E+ + E C S+ C + + N + I CE +R+ V TS ++ + +R K+ +PI +++++ + +G D +L + + TL++
Sbjct: 8 YEDQNYSALRRDCRRRKVLFEDPLFPATDDSLYYKGTPG--PAVRWKRPKGICEDPRLFVDGISSHDLHQGQVGNCWFVAACSSLASRESLWQKVIPDWKEQEWDPEKPNAYAGIFHFHFWRFGEWVDVVIDDRLPTVNNQLIYCHSNSRNEFWCALVEKAYAKLAGCYQALDGGNTADALVDFTGGVSEPI-DLTEGDFANDETKRNQLFERMLKVHSRGGLISASIKAVTAADMEARLACGLVKGHAYAVTDVRKVRLGHG-LLAFFKSEKLDMIRLRNPWGEREWNGPWSDTSEEWQKVSKSEREKMGVTVQDDGEFWMTFEDVCRYFTDIIKCRVINTSHLSIHKTWEEARLHGAWTLHEDPRQNRGGGCINHKDTFFQNPQYIFEVKKPEDEVLICIQQRPKRSTRREGKGENLAIGFDIYKVEENRQYRMHSLQHKAASSIYI--NSRSVFLRTDQPEGRYVIIPTTFEPGHTGEF----LLRVFTDVPSNC----RELRLDEPPHTCWSSLCGYPQLVTQVHVLGAAGLKDSPTGANSYVIIKCEGDKVRSAVQKGTSTPEYNVKGIFYRKKLSQPITVQVWNHRVLKDEFLGQVHLKADPDNL---QALHTLHL 612
BLAST of Calpain 5a vs. Ensembl Human
Match: CAPN5 (calpain 5 [Source:HGNC Symbol;Acc:HGNC:1482]) HSP 1 Score: 316.62 bits (810), Expect = 1.102e-96 Identity = 204/662 (30.82%), Postives = 324/662 (48.94%), Query Frame = 2
Query: 68 FNNQNYVSMRQEHQKNLTLFCDDTFGADDKSISPSGKQEILSSIEWKRPS----------------------------------------ELCINPCLFIAGSSVHDVCQGSLGNCWLVSALAVLAQHPECLKKVIPNVDDQEWHGNRPHSYTGMFRICLWRYGEWLEVVIDDRLPSRNGKLVFVHSKVKNEFWSALIEKAYAKVCGSYESLVGGLTCESMVDFTGGIPEQIFDLKK--YSKSDTEMENLFKLLHFDWKDGSLMCATI-ASRSDEMEEKLANGLITTHAYSITGVCKLRIKSTPMYEHYQKNHLMCIRLRNPWGQKEWNGSLSDASEEWHSISHKDKIAMGWKVENDGEFWMLIDDVLVNFNEITIIRPIKTGIFKYNSNWFGSMCKGYWTGVAGDRQSALA-------EIGQGGQFKIEINNPKGDIFLC------HLLQKCARGSEAALGILIIAVERNRDFMVHTPKFCNVKLEYRLGGSRSICLRYRLTCGTYIVKPFLTVASPSLKFPIYYLLRCYTTQKNNGTFLPSEMPILERNCICKSN-CTIIICRLHXXXXXXXXXXEELDVLNIFCEIDCENQSLRTTVNCNTSGLFWDETFLLHRSKMDRPIIIKIFHRGRFFNTLIGYSCCSYDSRHLNSPEVIETLNM 1882
+ +QNY ++R++ ++ LF D F A D S+ G ++ WKRP +C +P LF+ G S HD+ QG +GNCW V+A + LA +KVIP+ +QEW +P++Y G+F WR+GEW++VVIDDRLP+ N +L++ HS +NEFW AL+EKAYAK+ G Y++L GG T +++VDFTGG+ E I DL + ++ +T+ LF+ + G L+ A+I A + +ME +LA GL+ HAY++T V K+R+ + ++ L IRLRNPWG++EWNG SD SEEW +S ++ MG V++DGEFWM +DV F +I R I T + W + G WT RQ+ Q Q+ E+ P+ ++ +C ++ +G A+G I VE NR + +H+ + Y SRS+ LR G Y++ P + +F LLR +T +N E+ + E C S+ C + + N + I CE +R+ V TS ++ + +R K+ +PI +++++ + +G D +L + + TL++
Sbjct: 8 YEDQNYSALRRDCRRRKVLFEDPLFPATDDSLYYKGTPG--PAVRWKRPKYSGHSLAPHRPGWGDARRMENVPRPGSSGCPSSSLRARDLGICEDPRLFVDGISSHDLHQGQVGNCWFVAACSSLASRESLWQKVIPDWKEQEWDPEKPNAYAGIFHFHFWRFGEWVDVVIDDRLPTVNNQLIYCHSNSRNEFWCALVEKAYAKLAGCYQALDGGNTADALVDFTGGVSEPI-DLTEGDFANDETKRNQLFERMLKVHSRGGLISASIKAVTAADMEARLACGLVKGHAYAVTDVRKVRLGHG-LLAFFKSEKLDMIRLRNPWGEREWNGPWSDTSEEWQKVSKSEREKMGVTVQDDGEFWMTFEDVCRYFTDIIKCRVINTSHLSIHKTWEEARLHGAWTLHEDPRQNRGGGCINHKDTFFQNPQYIFEVKKPEDEVLICIQQRPKRSTRREGKGENLAIGFDIYKVEENRQYRMHSLQHKAASSIYI--NSRSVFLRTDQPEGRYVIIPTTFEPGHTGEF----LLRVFTDVPSNC----RELRLDEPPHTCWSSLCGYPQLVTQVHVLGAAGLKDSPTGANSYVIIKCEGDKVRSAVQKGTSTPEYNVKGIFYRKKLSQPITVQVWNHRVLKDEFLGQVHLKADPDNL---QALHTLHL 652
BLAST of Calpain 5a vs. Ensembl Human
Match: CAPN8 (calpain 8 [Source:HGNC Symbol;Acc:HGNC:1485]) HSP 1 Score: 254.603 bits (649), Expect = 7.765e-77 Identity = 143/345 (41.45%), Postives = 207/345 (60.00%), Query Frame = 2
Query: 50 SRNFVFFNNQNYVSMRQEHQKNLTLFCDDTFGA-----DDKSISPSGKQEILSSIEWKRPSELCINPCLFIAGSSVHDVCQGSLGNCWLVSALAVLAQHPECLKKVIPNVDDQEWHGNRPHSYTGMFRICLWRYGEWLEVVIDDRLPSRNGKLVFVHSKVKNEFWSALIEKAYAKVCGSYESLVGGLTCESMVDFTGGIPEQIFDLKKYSKSDTEMENLFKLLHFDWKDGSLM-CATIASRSDEMEEKLANGLITTHAYSITGVCKLRIKSTPMYEHYQKNHLMCIRLRNPWGQKEWNGSLSDASEEWHSISHKDKIAMGWKVENDGEFWMLIDDVLVNFNEITI 1066
++N + + Q++ ++RQ+ + LF D F A K + P Q I WKRP+ELC +P + G++ D+CQG LG+CWL++A+A L + E L +V+P DQ++ N Y G+F W+YGEW+EVVIDDRLP++NG+L+F+HS+ NEFWSAL+EKAYAK+ G YE+L GG T E DFTGGI E +DLKK NL++++ GSL+ C+ S + E E + L+ +HAYS+TGV ++ + P IRLRNPWG+ EW+G+ SD + EW+ I + K + KVE DGEFWM + D + F+ + I
Sbjct: 21 NQNALKYLGQDFKTLRQQCLDSGVLFKDPEFPACPSALGYKDLGPGSPQ--TQGIIWKRPTELCPSPQFIVGGATRTDICQGGLGDCWLLAAIASLTLNEELLYRVVPR--DQDFQEN----YAGIFHFQFWQYGEWVEVVIDDRLPTKNGQLLFLHSEQGNEFWSALLEKAYAKLNGCYEALAGGSTVEGFEDFTGGISE-FYDLKKPPA------NLYQIIRKALCAGSLLGCSIDVSSAAEAEAITSQKLVKSHAYSVTGVEEVNFQGHPE---------KLIRLRNPWGEVEWSGAWSDDAPEWNHIDPRRKEELDKKVE-DGEFWMSLSDFVRQFSRLEI 340
BLAST of Calpain 5a vs. Ensembl Celegans
Match: tra-3 (Calpain-5 [Source:UniProtKB/Swiss-Prot;Acc:Q22036]) HSP 1 Score: 316.62 bits (810), Expect = 1.916e-97 Identity = 204/600 (34.00%), Postives = 314/600 (52.33%), Query Frame = 2
Query: 68 FNNQNYVSMRQEHQKNLTLFCDDTFGADDKSISPSGKQEILSSIEWKRPSELCINPCLFIAGSSVHDVCQGSLGNCWLVSALAVLAQHPECLKKVIPNVDDQEWHGNRPHSYTGMFRICLWRYGEWLEVVIDDRLPSRNGKLVFVHSKVKNEFWSALIEKAYAKVCGSYESLVGGLTCESMVDFTGGIPEQIFDLK--KYSKSDTEMENLFKLLHFDWKDGSLMCATIASRS-DEMEEKLANGLITTHAYSITGVCKLRI-----KSTPMYEHYQKNHLMCIRLRNPWGQKEWNGSLSDASEEWHSISHKDKIAMGWKVEN----DGEFWMLIDDVLVNFNEITIIRPIKTGIFKYNSNWFGSMCKGYWT------GVAGDRQSA----LAEIGQGGQFKIEINNPKGDIFLCHLLQKCARGSE-----AALGILIIAVERNRDFMVHTPKFCNVKLEYRLGGSRSICLRYR-LTCGTYIVKPFLTVASPSLKFPIYYLLRCYTTQKNNGTFLPSEMPILERNCICKSNCTIIICRLHXXXXXXXXXXEELDVLNIFCEIDCENQSLRTTVNCNTSGLFWDETFLLHRSKMDRPIIIKIF 1783
F NQNY +R+ K F D F ++S+ +Q S I WKRP EL +P LF+ G+S +DV QG LGNCW VSA + L + + L +VIP+ DDQEW + H+Y G+FR WR+G+W+EVVIDD LP+R+GKL+F SK NEFWSAL+EKA+AK+ G YE+LVGG +++ D +GG+ E + K K +DTE++ LF L + G+L+ A IA+R+ +E+EE L GL+ HAY+++ VC + + +S + K IRL+NPWG+KEWNG+ SD S EW ++S MG + N DG+FWM + + F +I++ + T +F ++ ++ + WT G DR A Q+ +I +P + + + G + +G+ ++ VE NR + VHT +Y G RS+ L + L G Y++ P T +P K ++LR Y+ + + + L P L CKS ++ +H + + N++ + +S RT + WDE FL H+SK + I+++
Sbjct: 10 FGNQNYEKLRKICIKKKQPFVDTLFPPTNQSLFLEQRQS--SDIVWKRPGELHPDPHLFVEGASPNDVTQGILGNCWFVSACSALTHNFKLLAQVIPDADDQEW--STKHAYAGIFRFRFWRFGKWVEVVIDDLLPTRDGKLLFARSKTPNEFWSALLEKAFAKLYGCYENLVGGHLSDALQDVSGGVAETLHVRKFLKDDPNDTELK-LFNDLKTAFDKGALVVAAIAARTKEEIEESLDCGLVKGHAYAVSAVCTIDVTNPNERSFTSFIMGSKRKQNLIRLQNPWGEKEWNGAWSDDSPEWQNVSASQLSTMGVQPANSDSDDGDFWMPWESFVHYFTDISLCQLFNTSVFSFSRSYDEQIVFSEWTTNGKKSGAPDDRAGGCHNFKATFCNNPQYIFDIPSPNCSVMFALIQNDPSEGLKKREPFVTIGMHVMKVENNRQYRVHTAMHPIAISDYASG--RSVYLHLQSLPRGRYLLIP--TTFAP--KEQTLFMLRVYSDEHIHFSPLTKHAPKLGL-LKCKSAQSVTRLTIHGVDFNSASTGTH----NVYAILKDSRKSFRTKTLSGVKSIQWDEQFLFHKSKNRQQYKIEVW 593
BLAST of Calpain 5a vs. Ensembl Celegans
Match: clp-6 (CaLPain family [Source:UniProtKB/TrEMBL;Acc:Q9N4B2]) HSP 1 Score: 244.202 bits (622), Expect = 1.110e-70 Identity = 145/371 (39.08%), Postives = 202/371 (54.45%), Query Frame = 2
Query: 98 QEHQKNLT---LFCDDTFGADDKSISPSGKQEILSSIEWKRPSELCINPCLFIAGSSVHDVCQGSLGNCWLVSALAVLAQHPECLKKVIPNVDDQEWHGNRPHSYTGMFRICLWRYGEWLEVVIDDRLPSRNGKLVFVHSKVKNEFWSALIEKAYAKVCGSYESLVGGLTCESMVDFTGGIPEQIFDLKKYSKSDTEMENLFKLLHFDWKDGSLMCATIASRSDEMEEKLANGLITTHAYSITGVCKLRIKSTPMYEHYQKNHLMCIRLRNPWGQKEWNGSLSDASEEWHSISHKDKIAMGWKVENDGEFWMLIDDVLVNF-------------NEITIIRPIKTGIFKYNSNWFGSMCKGYW---TGVAG 1153
+E QK L LF D F A++ SI K+ S I W RP+++ NP L + G S DV QGSLGNCW ++A A LAQ E +V+P DQ + N Y G+F WRYGEW++VVIDDRLP+ NGKL+++ S+ +E W L+EKAYAK+ G+YE L GG T E++ DFTGG+ E +DL + T+ + ++ + GSL +I E E +LANGL+ HAYS+TGV + K + +R+RNPWG EWNG SD S W + + + M ++++ DGEFWM +DD + F +EIT + I+ + K + W G W G AG
Sbjct: 146 EERQKCLDENRLFEDPQFPANNSSIY--YKKPPKSHIVWLRPAKIFANPRLIVNGKSRFDVRQGSLGNCWFLAAAATLAQRDELFYRVVP--PDQSFTEN----YAGIFHFQFWRYGEWVDVVIDDRLPTVNGKLIYMSSQDGDECWGPLLEKAYAKLYGTYEHLDGGTTTEALEDFTGGLTE-FYDL-----TSTDKTMILAMIMKGMQMGSLFACSIDPDPREKEAQLANGLVRGHAYSVTGVHTVET---------DKEKVALLRIRNPWGDTEWNGDWSDKSSLWEQVDQEQREKMEFRIKEDGEFWMCLDDFMAQFANLDCCNLSADVMHEITEMTEIEV-MEKQSKQWIQKSADGEWCSRKGTAG 492
BLAST of Calpain 5a vs. Ensembl Celegans
Match: clp-1 (Calpain clp-1 [Source:UniProtKB/Swiss-Prot;Acc:P34308]) HSP 1 Score: 245.743 bits (626), Expect = 3.580e-70 Identity = 176/502 (35.06%), Postives = 253/502 (50.40%), Query Frame = 2
Query: 77 QNYVSMRQEHQKNLTLFCDDTFGADDKSISPSGKQEILSSIEWKRPSELCINPCLFIAGSSVHDVCQGSLGNCWLVSALAVLAQHPECLKKVIPNVDDQEWHGNRPHSYTGMFRICLWRYGEWLEVVIDDRLPSRNGKLVFVHSKVKNEFWSALIEKAYAKVCGSYESLVGGLTCESMVDFTGGIPEQIFDLKKYSKSDTEMENLFKLLHFDWKDGSLMCATIASRSDEMEEKLANGLITTHAYSITGVCKLRIKSTPMYEHYQKNHLMCI-RLRNPWG-QKEWNGSLSDASEEWHSISHKDKIAMGWKVENDGEFWMLIDDVLVNFNEITI--IRP-------IKTGIFKYNSNWFGSMCKGYWT--GVAGDRQSALAEIGQGGQFKIEINNPKGD------IFLCHLLQKCARG------SEAALGILIIAVERNRDFMVHTPKFCNVKLEYRLGGS---RSICLRYRLTCGTYIVKPFLTVASPSLKFPIYYLLRCYTT 1498
Q++ +R + ++ LF D F A+D S+ S + +EW RP E+ P L G S DV QG LG+CWL++A A L E +V+P DQ + N Y G+F W+YG+W++VVIDDRLP+ NG+L+++HS NEFWSAL+EKAYAK+ GSYE+L GG T E++ D TGG+ E I DLK + NL +++ ++ GSL +I + + E K++NGL+ HAYSITG RI P N CI R+RNPWG ++EWNG SD S EW S+ K MG K ++DGEFWM DD + NF ++ I + P TG+ W + G W AG ++ + QF++++ + D + +LQK R +G + NR + F K R R + R+R+ G Y+V P + +F +LR YT
Sbjct: 280 QDFYELRDQCLESKRLFEDPQFLANDSSLFFSKRPP--KRVEWLRPGEITREPQLITEGHSRFDVIQGELGDCWLLAAAANLTLKDELFYRVVP--PDQSFTEN----YAGIFHFQFWQYGKWVDVVIDDRLPTSNGELLYMHSASNNEFWSALLEKAYAKLFGSYEALKGGTTSEALEDMTGGLTEFI-DLKNPPR------NLMQMMMRGFEMGSLFGCSIEADPNVWEAKMSNGLVKGHAYSITGC---RIVDGP-------NGQTCILRIRNPWGNEQEWNGPWSDNSREWRSVPDSVKQDMGLKFDHDGEFWMSFDDFMRNFEKMEICNLGPDVMDEVYQMTGVKAAGMVWAANTHDGAWVRNQTAGGCRNYINTFANNPQFRVQLTDSDPDDDDELCTVIFAVLQKYRRNLKQDGLDNVPIGFAVYDAGNNRG-RLSKQFFAANKSAMRSAAFINLREMTGRFRVPPGNYVVVPSTFEPNEEAEF----MLRVYTN 751
BLAST of Calpain 5a vs. Ensembl Celegans
Match: clp-1 (Calpain clp-1 [Source:UniProtKB/Swiss-Prot;Acc:P34308]) HSP 1 Score: 245.743 bits (626), Expect = 5.391e-70 Identity = 176/502 (35.06%), Postives = 253/502 (50.40%), Query Frame = 2
Query: 77 QNYVSMRQEHQKNLTLFCDDTFGADDKSISPSGKQEILSSIEWKRPSELCINPCLFIAGSSVHDVCQGSLGNCWLVSALAVLAQHPECLKKVIPNVDDQEWHGNRPHSYTGMFRICLWRYGEWLEVVIDDRLPSRNGKLVFVHSKVKNEFWSALIEKAYAKVCGSYESLVGGLTCESMVDFTGGIPEQIFDLKKYSKSDTEMENLFKLLHFDWKDGSLMCATIASRSDEMEEKLANGLITTHAYSITGVCKLRIKSTPMYEHYQKNHLMCI-RLRNPWG-QKEWNGSLSDASEEWHSISHKDKIAMGWKVENDGEFWMLIDDVLVNFNEITI--IRP-------IKTGIFKYNSNWFGSMCKGYW--TGVAGDRQSALAEIGQGGQFKIEINNPKGD------IFLCHLLQKCARG------SEAALGILIIAVERNRDFMVHTPKFCNVKLEYRLGGS---RSICLRYRLTCGTYIVKPFLTVASPSLKFPIYYLLRCYTT 1498
Q++ +R + ++ LF D F A+D S+ S + +EW RP E+ P L G S DV QG LG+CWL++A A L E +V+P DQ + N Y G+F W+YG+W++VVIDDRLP+ NG+L+++HS NEFWSAL+EKAYAK+ GSYE+L GG T E++ D TGG+ E I DLK + NL +++ ++ GSL +I + + E K++NGL+ HAYSITG RI P N CI R+RNPWG ++EWNG SD S EW S+ K MG K ++DGEFWM DD + NF ++ I + P TG+ W + G W AG ++ + QF++++ + D + +LQK R +G + NR + F K R R + R+R+ G Y+V P + +F +LR YT
Sbjct: 301 QDFYELRDQCLESKRLFEDPQFLANDSSLFFSKRPP--KRVEWLRPGEITREPQLITEGHSRFDVIQGELGDCWLLAAAANLTLKDELFYRVVP--PDQSFTEN----YAGIFHFQFWQYGKWVDVVIDDRLPTSNGELLYMHSASNNEFWSALLEKAYAKLFGSYEALKGGTTSEALEDMTGGLTEFI-DLKNPPR------NLMQMMMRGFEMGSLFGCSIEADPNVWEAKMSNGLVKGHAYSITGC---RIVDGP-------NGQTCILRIRNPWGNEQEWNGPWSDNSREWRSVPDSVKQDMGLKFDHDGEFWMSFDDFMRNFEKMEICNLGPDVMDEVYQMTGVKAAGMVWAANTHDGAWVRNQTAGGCRNYINTFANNPQFRVQLTDSDPDDDDELCTVIFAVLQKYRRNLKQDGLDNVPIGFAVYDAGNNRG-RLSKQFFAANKSAMRSAAFINLREMTGRFRVPPGNYVVVPSTFEPNEEAEF----MLRVYTN 772
BLAST of Calpain 5a vs. Ensembl Celegans
Match: clp-1 (Calpain clp-1 [Source:UniProtKB/Swiss-Prot;Acc:P34308]) HSP 1 Score: 245.743 bits (626), Expect = 5.391e-70 Identity = 176/502 (35.06%), Postives = 253/502 (50.40%), Query Frame = 2
Query: 77 QNYVSMRQEHQKNLTLFCDDTFGADDKSISPSGKQEILSSIEWKRPSELCINPCLFIAGSSVHDVCQGSLGNCWLVSALAVLAQHPECLKKVIPNVDDQEWHGNRPHSYTGMFRICLWRYGEWLEVVIDDRLPSRNGKLVFVHSKVKNEFWSALIEKAYAKVCGSYESLVGGLTCESMVDFTGGIPEQIFDLKKYSKSDTEMENLFKLLHFDWKDGSLMCATIASRSDEMEEKLANGLITTHAYSITGVCKLRIKSTPMYEHYQKNHLMCI-RLRNPWG-QKEWNGSLSDASEEWHSISHKDKIAMGWKVENDGEFWMLIDDVLVNFNEITI--IRP-------IKTGIFKYNSNWFGSMCKGYW--TGVAGDRQSALAEIGQGGQFKIEINNPKGD------IFLCHLLQKCARG------SEAALGILIIAVERNRDFMVHTPKFCNVKLEYRLGGS---RSICLRYRLTCGTYIVKPFLTVASPSLKFPIYYLLRCYTT 1498
Q++ +R + ++ LF D F A+D S+ S + +EW RP E+ P L G S DV QG LG+CWL++A A L E +V+P DQ + N Y G+F W+YG+W++VVIDDRLP+ NG+L+++HS NEFWSAL+EKAYAK+ GSYE+L GG T E++ D TGG+ E I DLK + NL +++ ++ GSL +I + + E K++NGL+ HAYSITG RI P N CI R+RNPWG ++EWNG SD S EW S+ K MG K ++DGEFWM DD + NF ++ I + P TG+ W + G W AG ++ + QF++++ + D + +LQK R +G + NR + F K R R + R+R+ G Y+V P + +F +LR YT
Sbjct: 301 QDFYELRDQCLESKRLFEDPQFLANDSSLFFSKRPP--KRVEWLRPGEITREPQLITEGHSRFDVIQGELGDCWLLAAAANLTLKDELFYRVVP--PDQSFTEN----YAGIFHFQFWQYGKWVDVVIDDRLPTSNGELLYMHSASNNEFWSALLEKAYAKLFGSYEALKGGTTSEALEDMTGGLTEFI-DLKNPPR------NLMQMMMRGFEMGSLFGCSIEADPNVWEAKMSNGLVKGHAYSITGC---RIVDGP-------NGQTCILRIRNPWGNEQEWNGPWSDNSREWRSVPDSVKQDMGLKFDHDGEFWMSFDDFMRNFEKMEICNLGPDVMDEVYQMTGVKAAGMVWAANTHDGAWVRNQTAGGCRNYINTFANNPQFRVQLTDSDPDDDDELCTVIFAVLQKYRRNLKQDGLDNVPIGFAVYDAGNNRG-RLSKQFFAANKSAMRSAAFINLREMTGRFRVPPGNYVVVPSTFEPNEEAEF----MLRVYTN 772
BLAST of Calpain 5a vs. Ensembl Fly
Match: CalpA (gene:FBgn0012051 transcript:FBtr0086530) HSP 1 Score: 266.159 bits (679), Expect = 2.311e-79 Identity = 181/505 (35.84%), Postives = 268/505 (53.07%), Query Frame = 2
Query: 77 QNYVSMRQEHQKNLTLFCDDTFGADDKSISPSGKQEILSSIEWKRPSELCINPCLFIAGSSVHDVCQGSLGNCWLVSALAVLAQHPECLKKVIPNVDDQEWHGNRPHSYTGMFRICLWRYGEWLEVVIDDRLPSRNGKLVFVHSKVKNEFWSALIEKAYAKVCGSYESLVGGLTCESMVDFTGGIPEQIFDLKKYSKSDTEMENLFKLLHFDWKDGSLMCATIASRSDEMEEKLANGLITTHAYSITGVCKLRIKSTPMYEHYQKNHLMCIRLRNPWG-QKEWNGSLSDASEEWHSISHKDKIAMGWKVENDGEFWMLIDDVLVNFNEITI--IRPIKTGIFKYNSN---WFGSMCKGYWT-GV-AGDRQSALAEIGQGGQFKIEINNPKGD------IFLCHLLQKCARGSEA------ALGILIIAVERNRDFMVHTPKFCN-VKLEYRLGGS------RSICLRYRLTCGTYIVKPFLTVASPSLKFPIYYLLRCYTTQKNN 1510
Q+Y ++ + +LF D F A ++S+ S + + IEW RP E+ NP F+ G S DV QG LG+CWL++A A L Q +VIP +Q + N Y G+F W+YG+W++V+IDDRLP+ NG+L+++HS KNEFWSAL+EKAYAK+ GSYE+L GG TCE+M DFTGG+ E +DLK+ NLF +L + S+M +I + E + GLI HAYSIT VC + I TP ++ + IR+RNPWG + EWNG SD+S EW I + K +G + DGEFWM D L +F+ + I + P + NS W SM +G WT GV AG ++ L Q+ I + +P + + L+QK R +G I ++ N + + P+ N + + +G S R +C R++L G Y++ P + +F ++R ++ +NN
Sbjct: 73 QDYETILNSCLASGSLFEDPLFPASNESLQFSRRPD--RHIEWLRPHEIAENPQFFVEGYSRFDVQQGELGDCWLLAATANLTQESNLFFRVIPA--EQSFEEN----YAGIFHFRFWQYGKWVDVIIDDRLPTYNGELMYMHSTEKNEFWSALLEKAYAKLHGSYEALKGGSTCEAMEDFTGGVSE-WYDLKEAPG------NLFTILQKAAERNSMMGCSIEPDPNVTEAETPQGLIRGHAYSITKVCLIDI-VTPN----RQGKIPMIRMRNPWGNEAEWNGPWSDSSPEWRYIPEEQKAEIGLTFDRDGEFWMSFQDFLNHFDRVEICNLSPDSLTEDQQNSGKRKWEMSMYEGEWTPGVTAGGCRNFLDTFWHNPQYIITLVDPDEEDEEGQCTVIVALMQKNRRSKRNMGMECLTIGFAIYSL--NDRELENRPQGLNFFRYKSSVGRSPHFINTREVCARFKLPPGHYLIVPSTFDPNEEGEF----IIRVFSETQNN 551
BLAST of Calpain 5a vs. Ensembl Fly
Match: CalpA (gene:FBgn0012051 transcript:FBtr0112869) HSP 1 Score: 265.774 bits (678), Expect = 8.358e-77 Identity = 181/505 (35.84%), Postives = 268/505 (53.07%), Query Frame = 2
Query: 77 QNYVSMRQEHQKNLTLFCDDTFGADDKSISPSGKQEILSSIEWKRPSELCINPCLFIAGSSVHDVCQGSLGNCWLVSALAVLAQHPECLKKVIPNVDDQEWHGNRPHSYTGMFRICLWRYGEWLEVVIDDRLPSRNGKLVFVHSKVKNEFWSALIEKAYAKVCGSYESLVGGLTCESMVDFTGGIPEQIFDLKKYSKSDTEMENLFKLLHFDWKDGSLMCATIASRSDEMEEKLANGLITTHAYSITGVCKLRIKSTPMYEHYQKNHLMCIRLRNPWG-QKEWNGSLSDASEEWHSISHKDKIAMGWKVENDGEFWMLIDDVLVNFNEITI--IRPIKTGIFKYNSN---WFGSMCKGYWT-GV-AGDRQSALAEIGQGGQFKIEINNPKGD------IFLCHLLQKCARGSEA------ALGILIIAVERNRDFMVHTPKFCN-VKLEYRLGGS------RSICLRYRLTCGTYIVKPFLTVASPSLKFPIYYLLRCYTTQKNN 1510
Q+Y ++ + +LF D F A ++S+ S + + IEW RP E+ NP F+ G S DV QG LG+CWL++A A L Q +VIP +Q + N Y G+F W+YG+W++V+IDDRLP+ NG+L+++HS KNEFWSAL+EKAYAK+ GSYE+L GG TCE+M DFTGG+ E +DLK+ NLF +L + S+M +I + E + GLI HAYSIT VC + I TP ++ + IR+RNPWG + EWNG SD+S EW I + K +G + DGEFWM D L +F+ + I + P + NS W SM +G WT GV AG ++ L Q+ I + +P + + L+QK R +G I ++ N + + P+ N + + +G S R +C R++L G Y++ P + +F ++R ++ +NN
Sbjct: 73 QDYETILNSCLASGSLFEDPLFPASNESLQFSRRPD--RHIEWLRPHEIAENPQFFVEGYSRFDVQQGELGDCWLLAATANLTQESNLFFRVIPA--EQSFEEN----YAGIFHFRFWQYGKWVDVIIDDRLPTYNGELMYMHSTEKNEFWSALLEKAYAKLHGSYEALKGGSTCEAMEDFTGGVSE-WYDLKEAPG------NLFTILQKAAERNSMMGCSIEPDPNVTEAETPQGLIRGHAYSITKVCLIDI-VTP----NRQGKIPMIRMRNPWGNEAEWNGPWSDSSPEWRYIPEEQKAEIGLTFDRDGEFWMSFQDFLNHFDRVEICNLSPDSLTEDQQNSGKRKWEMSMYEGEWTPGVTAGGCRNFLDTFWHNPQYIITLVDPDEEDEEGQCTVIVALMQKNRRSKRNMGMECLTIGFAIYSL--NDRELENRPQGLNFFRYKSSVGRSPHFINTREVCARFKLPPGHYLIVPSTFDPNEEGEF----IIRVFSETQNN 551
BLAST of Calpain 5a vs. Ensembl Fly
Match: CalpA (gene:FBgn0012051 transcript:FBtr0086529) HSP 1 Score: 265.774 bits (678), Expect = 8.358e-77 Identity = 181/505 (35.84%), Postives = 268/505 (53.07%), Query Frame = 2
Query: 77 QNYVSMRQEHQKNLTLFCDDTFGADDKSISPSGKQEILSSIEWKRPSELCINPCLFIAGSSVHDVCQGSLGNCWLVSALAVLAQHPECLKKVIPNVDDQEWHGNRPHSYTGMFRICLWRYGEWLEVVIDDRLPSRNGKLVFVHSKVKNEFWSALIEKAYAKVCGSYESLVGGLTCESMVDFTGGIPEQIFDLKKYSKSDTEMENLFKLLHFDWKDGSLMCATIASRSDEMEEKLANGLITTHAYSITGVCKLRIKSTPMYEHYQKNHLMCIRLRNPWG-QKEWNGSLSDASEEWHSISHKDKIAMGWKVENDGEFWMLIDDVLVNFNEITI--IRPIKTGIFKYNSN---WFGSMCKGYWT-GV-AGDRQSALAEIGQGGQFKIEINNPKGD------IFLCHLLQKCARGSEA------ALGILIIAVERNRDFMVHTPKFCN-VKLEYRLGGS------RSICLRYRLTCGTYIVKPFLTVASPSLKFPIYYLLRCYTTQKNN 1510
Q+Y ++ + +LF D F A ++S+ S + + IEW RP E+ NP F+ G S DV QG LG+CWL++A A L Q +VIP +Q + N Y G+F W+YG+W++V+IDDRLP+ NG+L+++HS KNEFWSAL+EKAYAK+ GSYE+L GG TCE+M DFTGG+ E +DLK+ NLF +L + S+M +I + E + GLI HAYSIT VC + I TP ++ + IR+RNPWG + EWNG SD+S EW I + K +G + DGEFWM D L +F+ + I + P + NS W SM +G WT GV AG ++ L Q+ I + +P + + L+QK R +G I ++ N + + P+ N + + +G S R +C R++L G Y++ P + +F ++R ++ +NN
Sbjct: 73 QDYETILNSCLASGSLFEDPLFPASNESLQFSRRPD--RHIEWLRPHEIAENPQFFVEGYSRFDVQQGELGDCWLLAATANLTQESNLFFRVIPA--EQSFEEN----YAGIFHFRFWQYGKWVDVIIDDRLPTYNGELMYMHSTEKNEFWSALLEKAYAKLHGSYEALKGGSTCEAMEDFTGGVSE-WYDLKEAPG------NLFTILQKAAERNSMMGCSIEPDPNVTEAETPQGLIRGHAYSITKVCLIDI-VTP----NRQGKIPMIRMRNPWGNEAEWNGPWSDSSPEWRYIPEEQKAEIGLTFDRDGEFWMSFQDFLNHFDRVEICNLSPDSLTEDQQNSGKRKWEMSMYEGEWTPGVTAGGCRNFLDTFWHNPQYIITLVDPDEEDEEGQCTVIVALMQKNRRSKRNMGMECLTIGFAIYSL--NDRELENRPQGLNFFRYKSSVGRSPHFINTREVCARFKLPPGHYLIVPSTFDPNEEGEF----IIRVFSETQNN 551
BLAST of Calpain 5a vs. Ensembl Fly
Match: CalpA (gene:FBgn0012051 transcript:FBtr0340532) HSP 1 Score: 265.774 bits (678), Expect = 1.085e-76 Identity = 181/505 (35.84%), Postives = 268/505 (53.07%), Query Frame = 2
Query: 77 QNYVSMRQEHQKNLTLFCDDTFGADDKSISPSGKQEILSSIEWKRPSELCINPCLFIAGSSVHDVCQGSLGNCWLVSALAVLAQHPECLKKVIPNVDDQEWHGNRPHSYTGMFRICLWRYGEWLEVVIDDRLPSRNGKLVFVHSKVKNEFWSALIEKAYAKVCGSYESLVGGLTCESMVDFTGGIPEQIFDLKKYSKSDTEMENLFKLLHFDWKDGSLMCATIASRSDEMEEKLANGLITTHAYSITGVCKLRIKSTPMYEHYQKNHLMCIRLRNPWG-QKEWNGSLSDASEEWHSISHKDKIAMGWKVENDGEFWMLIDDVLVNFNEITI--IRPIKTGIFKYNSN---WFGSMCKGYWT-GV-AGDRQSALAEIGQGGQFKIEINNPKGD------IFLCHLLQKCARGSEA------ALGILIIAVERNRDFMVHTPKFCN-VKLEYRLGGS------RSICLRYRLTCGTYIVKPFLTVASPSLKFPIYYLLRCYTTQKNN 1510
Q+Y ++ + +LF D F A ++S+ S + + IEW RP E+ NP F+ G S DV QG LG+CWL++A A L Q +VIP +Q + N Y G+F W+YG+W++V+IDDRLP+ NG+L+++HS KNEFWSAL+EKAYAK+ GSYE+L GG TCE+M DFTGG+ E +DLK+ NLF +L + S+M +I + E + GLI HAYSIT VC + I TP ++ + IR+RNPWG + EWNG SD+S EW I + K +G + DGEFWM D L +F+ + I + P + NS W SM +G WT GV AG ++ L Q+ I + +P + + L+QK R +G I ++ N + + P+ N + + +G S R +C R++L G Y++ P + +F ++R ++ +NN
Sbjct: 88 QDYETILNSCLASGSLFEDPLFPASNESLQFSRRPD--RHIEWLRPHEIAENPQFFVEGYSRFDVQQGELGDCWLLAATANLTQESNLFFRVIPA--EQSFEEN----YAGIFHFRFWQYGKWVDVIIDDRLPTYNGELMYMHSTEKNEFWSALLEKAYAKLHGSYEALKGGSTCEAMEDFTGGVSE-WYDLKEAPG------NLFTILQKAAERNSMMGCSIEPDPNVTEAETPQGLIRGHAYSITKVCLIDI-VTP----NRQGKIPMIRMRNPWGNEAEWNGPWSDSSPEWRYIPEEQKAEIGLTFDRDGEFWMSFQDFLNHFDRVEICNLSPDSLTEDQQNSGKRKWEMSMYEGEWTPGVTAGGCRNFLDTFWHNPQYIITLVDPDEEDEEGQCTVIVALMQKNRRSKRNMGMECLTIGFAIYSL--NDRELENRPQGLNFFRYKSSVGRSPHFINTREVCARFKLPPGHYLIVPSTFDPNEEGEF----IIRVFSETQNN 566
BLAST of Calpain 5a vs. Ensembl Fly
Match: CalpB (gene:FBgn0025866 transcript:FBtr0076343) HSP 1 Score: 266.544 bits (680), Expect = 1.538e-76 Identity = 182/505 (36.04%), Postives = 269/505 (53.27%), Query Frame = 2
Query: 80 NYVSMRQEHQKNLTLFCDDTFGADDKSISPSGKQEILSSIEWKRPSELCINPCLFIAGSSVHDVCQGSLGNCWLVSALAVLAQHPECLKKVIPNVDDQEWHGNRPHSYTGMFRICLWRYGEWLEVVIDDRLPSRNGKLVFVHSKVKNEFWSALIEKAYAKVCGSYESLVGGLTCESMVDFTGGIPEQIFDLKKYSKSDTEMENLFKLLHFDWKDGSLMCATIASRSDEMEEKLANGLITTHAYSITGVCKLRIKSTPMYEHYQKNHLMCIRLRNPWG-QKEWNGSLSDASEEWHSISHKDKIAMGWKVENDGEFWMLIDDVLVNFNEITI--IRPIKTGIFKYNSN---WFGSMCKGYWTG--VAGDRQSALAEIGQGGQFKIEINNPKGD------IFLCHLLQKCARGSEAALGILIIAVE------RNRDFMVHTPKFCNVKLEYRLGGSRS--------ICLRYRLTCGTYIVKPFLTVASPSLKFPIYYLLRCYTTQKNN 1510
++ S+R N T+F D F A + S+ S + + EW RP ++ +P F+ G S DV QG LG+CWL++A A L Q +VIP DQ++ N Y G+F W+YG+W+EVVIDDRLP+ NG+L+++HS KNEFWSAL+EKAYAK+ GSYE+L GG TCE+M DFTGG+ E +D+K+ NLF ++ + GS+M ++ +E + GLI HAYSIT VC + I STP ++ L IR+RNPWG EW+G SD+S EW I K +G + DGEFWM D L +F+ + I + P + +S+ W SM +G WT AG ++ L Q+ I + +P + + L+QK R S+ +GI + + +RD V P+ N +YR +RS +C R++L G Y++ P + +F ++R ++ +NN
Sbjct: 245 DFQSLRDSCLANGTMFEDPDFPATNASLMYSRRPDRY--YEWLRPGDIADDPQFFVEGYSRFDVQQGELGDCWLLAAAANLTQDSTLFFRVIP--PDQDFQEN----YAGIFHFKFWQYGKWVEVVIDDRLPTYNGELIYMHSTEKNEFWSALLEKAYAKLHGSYEALKGGTTCEAMEDFTGGVTE-WYDIKEAPP------NLFSIMMKAAERGSMMGCSLEPDPHVLEAETPQGLIRGHAYSITKVCLMDI-STPN----RQGKLPMIRMRNPWGNDAEWSGPWSDSSPEWRFIPEHTKEEIGLNFDRDGEFWMSFQDFLNHFDRVEICNLSPDSLTEDQQHSSRRKWEMSMFEGEWTSGVTAGGCRNFLETFWHNPQYIISLEDPDDEDDDGKCTAIVALMQKNRR-SKRNVGIDCLTIGFAIYHLTDRDMQVK-PQGLNF-FKYRASVARSPHFINTREVCARFKLPPGHYLIVPSTFDPNEEGEF----IIRVFSETRNN 722
BLAST of Calpain 5a vs. Ensembl Zebrafish
Match: capn5a (calpain 5a [Source:ZFIN;Acc:ZDB-GENE-061103-214]) HSP 1 Score: 360.918 bits (925), Expect = 3.486e-114 Identity = 213/606 (35.15%), Postives = 343/606 (56.60%), Query Frame = 2
Query: 68 FNNQNYVSMRQEHQKNLTLFCDDTFGADDKSISPSGKQEILSSIEWKRPSELCINPCLFIAGSSVHDVCQGSLGNCWLVSALAVLAQHPECLKKVIPNVDDQEWHGNRPHSYTGMFRICLWRYGEWLEVVIDDRLPSRNGKLVFVHSKVKNEFWSALIEKAYAKVCGSYESLVGGLTCESMVDFTGGIPEQIFDLK-KYSKSDTEMENLF-KLLHFDWKDGSLMCATIASRSDEMEEKLANGLITTHAYSITGVCKLRIKSTPMYEHYQKNHLMCIRLRNPWGQKEWNGSLSDASEEWHSISHKDKIAMGWKVENDGEFWMLIDDVLVNFNEITIIRPIKTGIFKYNSNWFGSMCKGYWT-------GVAGDRQSALAEIGQGGQFKIEINNPKGDIFLCHLLQKC---ARGSEAALGILIIAVERNRDFMVHTPKFCNVKLEYRLGG-----SRSICLRYRLTCGTYIVKPFLTVASPSLKFPIYYLLRCYTTQKNNGTFLPS---EMPILERNCICKSNC---TIIICRLHXXXXXXXXXXEELDVLNIFCEIDCENQSLRTTVNCNTSGLFWDETFLLHRSKMDRPIIIKIFHRGRFFNTLIG 1816
F Q + ++R++ Q+N TLF D F A+D+S+ G + L + WKRP +LC +P LF+ G S HD+ QG LGNCW V+A + LA +KVIP+ DQEW N+P SY G+F WR+G+W++VVIDDRLP+ NGKL++ HS NEFWSAL+EKAYAK+CG YE+L GG T +++VDFTGG+ E + L+ K+++ + LF ++L + G + C+ A+ +ME +L GL+ HAY++T V K+R+ S + ++ L IR+RNPWGQ+EWNG+ SD+SEEW +S ++ +G VE+DGEFWM DD +F ++ + R I T + W + +G W+ +G + Q Q+ ++ + ++ +C Q A+G A+G I VE NR + +H+ ++ +G SRS+ LR L G Y++ P + P + P +LLR +T +PS E+ + E C ++C ++ ++H+I+AD ++A + + + I CE + + + V+ +T +D + +R K +PI I+I+++ ++ +G
Sbjct: 8 FEGQEFSALRKQCQQNHTLFEDPVFRAEDQSLFLQGNR--LGKVTWKRPKDLCEDPHLFVNGISAHDLNQGQLGNCWFVAACSSLASREALWQKVIPDWKDQEWDKNKPESYAGIFHFRFWRFGDWVDVVIDDRLPTANGKLIYCHSNDSNEFWSALVEKAYAKMCGCYEALDGGNTADALVDFTGGVSEPMDLLEGKFAQDEEARHQLFDRVLKVHNRGGLISCSIRATTQADMEARLDCGLVKGHAYAVTDVRKVRLGSG-LLAFFKSEKLSMIRMRNPWGQREWNGAWSDSSEEWKRVSKGERERLGVTVEDDGEFWMDFDDFCKHFTDLILCRLINTSYLSIHKTWEEEVMRGCWSRHDDPLRNRSGGCINHKTTFLQNPQYVFDVTKAEDEVLICLQQQDKRARAKGENMAIGFDIQRVELNRIYRMHS-------IQKSVGSSIYINSRSVFLRKDLKEGRYVIVP--STFDPGM--PGDFLLRIFTD-------VPSDCKELTVDEPPQTCWTSCCGYPKLVTQVHVIRADGLKALDGDGSSDPYIIISCEGEKVCSPVHKDTQSPDFDVKAVFYRKKPKQPIRIQIYNKNLLSDSFMG 592
BLAST of Calpain 5a vs. Ensembl Zebrafish
Match: capn5a (calpain 5a [Source:ZFIN;Acc:ZDB-GENE-061103-214]) HSP 1 Score: 360.918 bits (925), Expect = 3.486e-114 Identity = 213/606 (35.15%), Postives = 343/606 (56.60%), Query Frame = 2
Query: 68 FNNQNYVSMRQEHQKNLTLFCDDTFGADDKSISPSGKQEILSSIEWKRPSELCINPCLFIAGSSVHDVCQGSLGNCWLVSALAVLAQHPECLKKVIPNVDDQEWHGNRPHSYTGMFRICLWRYGEWLEVVIDDRLPSRNGKLVFVHSKVKNEFWSALIEKAYAKVCGSYESLVGGLTCESMVDFTGGIPEQIFDLK-KYSKSDTEMENLF-KLLHFDWKDGSLMCATIASRSDEMEEKLANGLITTHAYSITGVCKLRIKSTPMYEHYQKNHLMCIRLRNPWGQKEWNGSLSDASEEWHSISHKDKIAMGWKVENDGEFWMLIDDVLVNFNEITIIRPIKTGIFKYNSNWFGSMCKGYWT-------GVAGDRQSALAEIGQGGQFKIEINNPKGDIFLCHLLQKC---ARGSEAALGILIIAVERNRDFMVHTPKFCNVKLEYRLGG-----SRSICLRYRLTCGTYIVKPFLTVASPSLKFPIYYLLRCYTTQKNNGTFLPS---EMPILERNCICKSNC---TIIICRLHXXXXXXXXXXEELDVLNIFCEIDCENQSLRTTVNCNTSGLFWDETFLLHRSKMDRPIIIKIFHRGRFFNTLIG 1816
F Q + ++R++ Q+N TLF D F A+D+S+ G + L + WKRP +LC +P LF+ G S HD+ QG LGNCW V+A + LA +KVIP+ DQEW N+P SY G+F WR+G+W++VVIDDRLP+ NGKL++ HS NEFWSAL+EKAYAK+CG YE+L GG T +++VDFTGG+ E + L+ K+++ + LF ++L + G + C+ A+ +ME +L GL+ HAY++T V K+R+ S + ++ L IR+RNPWGQ+EWNG+ SD+SEEW +S ++ +G VE+DGEFWM DD +F ++ + R I T + W + +G W+ +G + Q Q+ ++ + ++ +C Q A+G A+G I VE NR + +H+ ++ +G SRS+ LR L G Y++ P + P + P +LLR +T +PS E+ + E C ++C ++ ++H+I+AD ++A + + + I CE + + + V+ +T +D + +R K +PI I+I+++ ++ +G
Sbjct: 8 FEGQEFSALRKQCQQNHTLFEDPVFRAEDQSLFLQGNR--LGKVTWKRPKDLCEDPHLFVNGISAHDLNQGQLGNCWFVAACSSLASREALWQKVIPDWKDQEWDKNKPESYAGIFHFRFWRFGDWVDVVIDDRLPTANGKLIYCHSNDSNEFWSALVEKAYAKMCGCYEALDGGNTADALVDFTGGVSEPMDLLEGKFAQDEEARHQLFDRVLKVHNRGGLISCSIRATTQADMEARLDCGLVKGHAYAVTDVRKVRLGSG-LLAFFKSEKLSMIRMRNPWGQREWNGAWSDSSEEWKRVSKGERERLGVTVEDDGEFWMDFDDFCKHFTDLILCRLINTSYLSIHKTWEEEVMRGCWSRHDDPLRNRSGGCINHKTTFLQNPQYVFDVTKAEDEVLICLQQQDKRARAKGENMAIGFDIQRVELNRIYRMHS-------IQKSVGSSIYINSRSVFLRKDLKEGRYVIVP--STFDPGM--PGDFLLRIFTD-------VPSDCKELTVDEPPQTCWTSCCGYPKLVTQVHVIRADGLKALDGDGSSDPYIIISCEGEKVCSPVHKDTQSPDFDVKAVFYRKKPKQPIRIQIYNKNLLSDSFMG 592
BLAST of Calpain 5a vs. Ensembl Zebrafish
Match: capn5a (calpain 5a [Source:ZFIN;Acc:ZDB-GENE-061103-214]) HSP 1 Score: 345.51 bits (885), Expect = 1.432e-109 Identity = 191/507 (37.67%), Postives = 292/507 (57.59%), Query Frame = 2
Query: 68 FNNQNYVSMRQEHQKNLTLFCDDTFGADDKSISPSGKQEILSSIEWKRPSELCINPCLFIAGSSVHDVCQGSLGNCWLVSALAVLAQHPECLKKVIPNVDDQEWHGNRPHSYTGMFRICLWRYGEWLEVVIDDRLPSRNGKLVFVHSKVKNEFWSALIEKAYAKVCGSYESLVGGLTCESMVDFTGGIPEQIFDLK-KYSKSDTEMENLF-KLLHFDWKDGSLMCATIASRSDEMEEKLANGLITTHAYSITGVCKLRIKSTPMYEHYQKNHLMCIRLRNPWGQKEWNGSLSDASEEWHSISHKDKIAMGWKVENDGEFWMLIDDVLVNFNEITIIRPIKTGIFKYNSNWFGSMCKGYWT-------GVAGDRQSALAEIGQGGQFKIEINNPKGDIFLCHLLQKC---ARGSEAALGILIIAVERNRDFMVHTPKFCNVKLEYRLGG-----SRSICLRYRLTCGTYIVKPFLTVASPSLKFPIYYLLRCYTTQKNNGTFLPSEMP 1537
F Q + ++R++ Q+N TLF D F A+D+S+ G + L + WKRP +LC +P LF+ G S HD+ QG LGNCW V+A + LA +KVIP+ DQEW N+P SY G+F WR+G+W++VVIDDRLP+ NGKL++ HS NEFWSAL+EKAYAK+CG YE+L GG T +++VDFTGG+ E + L+ K+++ + LF ++L + G + C+ A+ +ME +L GL+ HAY++T V K+R+ S + ++ L IR+RNPWGQ+EWNG+ SD+SEEW +S ++ +G VE+DGEFWM DD +F ++ + R I T + W + +G W+ +G + Q Q+ ++ + ++ +C Q A+G A+G I VE NR + +H+ ++ +G SRS+ LR L G Y++ P + P + P +LLR +T ++ L + P
Sbjct: 8 FEGQEFSALRKQCQQNHTLFEDPVFRAEDQSLFLQGNR--LGKVTWKRPKDLCEDPHLFVNGISAHDLNQGQLGNCWFVAACSSLASREALWQKVIPDWKDQEWDKNKPESYAGIFHFRFWRFGDWVDVVIDDRLPTANGKLIYCHSNDSNEFWSALVEKAYAKMCGCYEALDGGNTADALVDFTGGVSEPMDLLEGKFAQDEEARHQLFDRVLKVHNRGGLISCSIRATTQADMEARLDCGLVKGHAYAVTDVRKVRLGSG-LLAFFKSEKLSMIRMRNPWGQREWNGAWSDSSEEWKRVSKGERERLGVTVEDDGEFWMDFDDFCKHFTDLILCRLINTSYLSIHKTWEEEVMRGCWSRHDDPLRNRSGGCINHKTTFLQNPQYVFDVTKAEDEVLICLQQQDKRARAKGENMAIGFDIQRVELNRIYRMHS-------IQKSVGSSIYINSRSVFLRKDLKEGRYVIVP--STFDPGM--PGDFLLRIFTDVPSDCKELTVDEP 500
BLAST of Calpain 5a vs. Ensembl Zebrafish
Match: capn5b (calpain 5b [Source:ZFIN;Acc:ZDB-GENE-091116-29]) HSP 1 Score: 327.02 bits (837), Expect = 3.102e-101 Identity = 202/607 (33.28%), Postives = 330/607 (54.37%), Query Frame = 2
Query: 68 FNNQNYVSMRQEHQKNLTLFCDDTFGADDKSISPSGKQEILSSIEWKRPSELCINPCLFIAGSSVHDVCQGSLGNCWLVSALAVLAQHPECLKKVIPNVDDQEWHGNRPHSYTGMFRICLWRYGEWLEVVIDDRLPSRNGKLVFVHSKVKNEFWSALIEKAYAKVCGSYESLVGGLTCESMVDFTGGIPEQIFDLK-KYSKSDTEMENLF-KLLHFDWKDGSLMCATIASRSDEMEEKLANGLITTHAYSITGVCKLRIKSTPMYEHYQKNHLMCIRLRNPWGQKEWNGSLSDASEEWHSISHKDKIAMGWKVENDGEFWMLIDDVLVNFNEITIIRPIKTGIFKYNSNWFGSMCKGYW-------TGVAGDRQSALAEIGQGGQFKIEINNPKGDIFLCHLLQKCAR-------GSEAALGILIIAVERNRDFMVHTPKFCNVKLEYRLGG-----SRSICLRYRLTCGTYIVKPFLTVASPSLKFPIYYLLRCYTTQKNNGTFLPSEMPILERNC---ICKSNCTIIICRLHXXXXXXXXXXEELDVLNIFCEIDCENQSLRTTVNCNTSGLFWDETFLLHRSKMDRPIIIKIFHRGRFFNTLIG 1816
F Q+Y +++++ ++ LF D F A D S+ G + + + WKRP ELC +P LF+ G S HD+ QG LGNCW V+A + LA +KVIP+ +QEW + SY G+F WR+GEW++VVIDDRLP+ + +LV+ HS NEFWSAL+EKAYAKV G YE+L GG T +++VDFTGG+ E + L+ ++++ +T LF ++L +DG + C+ A+ ++ME +L GL+ HAY++T V K+R+ + +++ L IR+RNPWG+KEW+G SD+SEEW +S ++ +G V++DGEFWM +D F ++ + R I T + W + +G W AG + Q Q+ ++ + ++ +C L QK R G A+G I VE NR + +H+ + ++ G SR + LR L G Y++ P T P + +LLR +T ++ L + P + C +C ++ ++H++ A+ +Q + + + I CE + +R+ V+ +T +D + +R K I I+I+++ +T +G
Sbjct: 8 FEGQHYSTLKRQCLQSGALFEDPLFPAVDDSLFYQGNR--IGRVHWKRPEELCDDPHLFVDGISAHDLHQGQLGNCWFVAACSSLASRESLWQKVIPDWKEQEWDPEKADSYAGIFHFRFWRFGEWVDVVIDDRLPTVDNQLVYCHSNDSNEFWSALVEKAYAKVYGCYEALDGGNTADALVDFTGGVSEPMDLLEGQFAQDETARNQLFERVLKVHNRDGLISCSIRATTVEDMEARLDCGLVKGHAYAVTDVRKVRL-GHGLLAYFKSEKLHMIRMRNPWGEKEWSGPWSDSSEEWKKVSKSEREKLGVTVQDDGEFWMNFEDFCHYFTDLILCRLINTSYLSIHKTWEEEVMRGLWLHRDEPLRNRAGGCINHKTTFLQNPQYVFDVRKVEDEVLIC-LQQKERRATPKEGKGENLAIGFDIHRVELNRKYRMHSA-------QQKVAGSIYINSRCVFLRKELKEGRYVIIP--TTFDPGQQGE--FLLRVFTDVPSDCKELTLDEP--PQTCWTGMC--GYPQLVTQVHVLSAEGLQGQDANGASDPYVIITCEGEKVRSPVHKDTRCPNFDIKGIFYRKKPKEGIHIEIYNKNVIVDTFLG 595
BLAST of Calpain 5a vs. Ensembl Zebrafish
Match: BX324225.2 (calpain 5b [Source:NCBI gene;Acc:100006332]) HSP 1 Score: 320.087 bits (819), Expect = 1.404e-98 Identity = 196/594 (33.00%), Postives = 321/594 (54.04%), Query Frame = 2
Query: 68 FNNQNYVSMRQEHQKNLTLFCDDTFGADDKSISPSGKQEILSSIEWKRPSELCINPCLFIAGSSVHDVCQGSLGNCWLVSALAVLAQHPECLKKVIPNVDDQEWHGNRPHSYTGMFRICLWRYGEWLEVVIDDRLPSRNGKLVFVHSKVKNEFWSALIEKAYAKVCGSYESLVGGLTCESMVDFTGGIPEQIFDLK-KYSKSDTEMENLF-KLLHFDWKDGSLMCATIASRSDEMEEKLANGLITTHAYSITGVCKLRIKSTPMYEHYQKNHLMCIRLRNPWGQKEWNGSLSDASEEWHSISHKDKIAMGWKVENDGEFWMLIDDVLVNFNEITIIRPIKTGIFKYNSNWFGSMCKGYW-------TGVAGDRQSALAEIGQGGQFKIEINNPKGDIFLC------HLLQKCARGSEAALGILIIAVERNRDFMVHTPKFCNVKLEYRLGG-----SRSICLRYRLTCGTYIVKPFLTVASPSLKFPIYYLLRCYTTQKNNGTFLPSEMPILERNC---ICKSNCTIIICRLHXXXXXXXXXXEELDVLNIFCEIDCENQSLRTTVNCNTSGLFWDETFLLHRSKMDRPIIIKI 1780
F Q+Y +++++ ++ LF D F A D S+ G + + + WKRP ELC +P LF+ G S HD+ QG LGNCW V+A + LA +KVIP+ +QEW + SY G+F WR+GEW++VVIDDRLP+ + +LV+ HS NEFWSAL+EKAYAKV G YE+L GG T +++VDFTGG+ E + L+ ++++ +T LF ++L +DG + C+ A+ ++ME +L GL+ HAY++T V K+R+ + +++ L IR+RNPWG+KEW+G SD+SEEW +S ++ +G V++DGEFWM +D F ++ + R I T + W + +G W AG + Q Q+ ++ + ++ +C K +G A+G I VE NR + +H+ + ++ G SR + LR L G Y++ P T P + +LLR +T ++ L + P + C +C ++ ++H++ A+ +Q + + + I CE + +R+ V+ +T +D + +R K I I++
Sbjct: 8 FEGQHYSTLKRQCLQSGALFEDPLFPAVDDSLFYQGNR--IGRVHWKRPEELCDDPHLFVDGISAHDLHQGQLGNCWFVAACSSLASRESLWQKVIPDWKEQEWDPEKADSYAGIFHFRFWRFGEWVDVVIDDRLPTVDNQLVYCHSNDSNEFWSALVEKAYAKVYGCYEALDGGNTADALVDFTGGVSEPMDLLEGQFAQDETARNQLFERVLKVHNRDGLISCSIRATTVEDMEARLDCGLVKGHAYAVTDVRKVRLGHG-LLAYFKSEKLHMIRMRNPWGEKEWSGPWSDSSEEWKKVSKSEREKLGVTVQDDGEFWMNFEDFCHYFTDLILCRLINTSYLSIHKTWEEEVMRGLWLHRDEPLRNRAGGCINHKTTFLQNPQYVFDVRKVEDEVLICLQQKERRATPKEGKGENLAIGFDIHRVELNRKYRMHSA-------QQKVAGSIYINSRCVFLRKELKEGRYVIIP--TTFDPGQQGE--FLLRVFTDVPSDCKELTLDEP--PQTCWTGMC--GYPQLVTQVHVLSAEGLQGQDANGASDPYVIITCEGEKVRSPVHKDTRCPNFDIKGIFYRKKPKEGIHIEV 583
BLAST of Calpain 5a vs. Ensembl Xenopus
Match: ppa2 (pyrophosphatase (inorganic) 2 [Source:Xenbase;Acc:XB-GENE-961143]) HSP 1 Score: 312.383 bits (799), Expect = 2.110e-96 Identity = 189/505 (37.43%), Postives = 267/505 (52.87%), Query Frame = 2
Query: 68 FNNQNYVSMRQEHQKNLTLFCDDTFGADDKSISPSGKQEILSSIEWKRPSELCINPCLFIAGSSVHDVCQGSLGNCWLVSALAVLAQHPECLKKVIPNVDDQEWHGNRPHSYTGMFRICLWRYGEWLEVVIDDRLPSRNGKLVFVHSKVKNEFWSALIEKAYAKVCGSYESLVGGLTCESMVDFTGGIPEQIFDLKK--YSKSDTEMENLFKLLHFDWKDGSLM-CATIASRSDEMEEKLANGLITTHAYSITGVCKLRIKSTPMYEHYQKNHLMCIRLRNPWGQKEWNGSLSDASEEWHSISHKDKIAMGWKVENDGEFWMLIDDVLVNFNEITIIRPIKTGIFKYNSNWFGSMCKGYW---TGVAGDRQSAL---AEIGQGGQFKIEINNPKGDIFLC------HLLQKCARGSEAALGILIIAVERNRDFMVHTPKFCNVKLEYRLGGSRSICLRYRLTCGTYIVKPFLTVASPSLKFPIYYLLRCYTTQKNNGTFLPSEMP 1537
QNY +++E KN LF D F A D+S+ S ++ IEWKRP ELC +P LF+ G S HD+ QG LGNCW V+A + LA KVI N QEW RPH Y G+F W G+W++VVIDDRLPS +G LV+ HS VKNEFWSAL+EKAYAK+ GSYE+L GG T +++VDFTG + E F+L K Y S E LF+ L ++ G L+ C S++ME + GL+ HAYS+T + K+ + + N L IR+RNPWG++EW G+ SD SEEW+ +S +K +G V +DGEFWM +D ++F + R + T W G W T +R Q Q+ E+ + ++ + + +K +G+ +G I VE NR++ +H L R+I LR LT G Y++ P T P + ++LR +T + + L + P
Sbjct: 8 LKTQNYHELKKECIKNGKLFEDPEFPASDESLFYSKAPDL--RIEWKRPKELCEDPHLFVDGISSHDLHQGRLGNCWFVAACSCLALKKALWTKVISNWKKQEWDPKRPHRYAGIFHFKFWCLGDWVDVVIDDRLPSVDGNLVYCHSNVKNEFWSALLEKAYAKLAGSYEALDGGNTADAIVDFTGAVTES-FELDKGDYRTSLMEQTKLFQELLKVYRAGGLISCYIKPVSSNDMEAVTSYGLVKGHAYSVTAIKKMPVGQRILC-FGNSNKLFMIRMRNPWGKREWKGAWSDESEEWNKVSRSEKATLGLTVADDGEFWMTFEDWCLSFTHADVCRILNTSYLSCYKTWKQEEVHGAWSKNTDPLLNRSGGCFNETTFFQNPQYIFEVRKKEDEVLISLQQKDQRIHKKDGKGNNFIIGFEIFKVELNREYRIHKISGQEKTLRSSYINLRAIFLRKVLTHGRYVIIP--TTFHPGISSS--FILRLFTDESSRFRELLLDKP 504
BLAST of Calpain 5a vs. Ensembl Xenopus
Match: ENSXETT00000037551.1 (pep primary_assembly:Xenopus_tropicalis_v9.1:KV460378.1:547358:568026:1 gene:ENSXETG00000014732.1 transcript:ENSXETT00000037551.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 294.278 bits (752), Expect = 4.513e-89 Identity = 190/523 (36.33%), Postives = 270/523 (51.63%), Query Frame = 2
Query: 41 MDFSRNFVF-FNNQNYVSMRQEHQKNLTLFCDDTFGADDKSISPSGKQEILSSIEWKRPSELCINPCLFIAGS-SVHDVCQGSLGNCWLVSALAVLAQHPECLKKVIPNVDDQEWH--GNRPHSYTGMFRICLWRYGEWLEVVIDDRLPSRNGKLVFVHSKVKNEFWSALIEKAYAKVCGSYESLVGGLTCESMVDFTGGIPEQIFDLKKYSKSDTEMENLFKLLHFDWKDGSLMCATIASRSDE-MEEKLANGLITTHAYSITGVCKLRIKSTPMYEHYQKNHLMCIRLRNPWGQKEWNGSLSDASEEWHSISHKDKIAMGWKVENDGEFWMLIDDVLVNFNEITIIRPIKTGIFK-------------------YNSNWFGSMCKGYWTGVAGDRQSALAEIGQGGQFKIEINNPKGDIFLCHLLQKCARGSEAALGILIIAVERNRDFMVHTPKFCNVKLEYRLGGSRSICLRYRLTCGTYIVKPFLTVASPSLKFPIYYLLRCYTTQKNNGTFLPSEMP 1537
M FS V + +QNY ++ ++ LF D F AD +S+ S + +EWKRP EL NP LF G S HD+ QGSLGNCW V+A A LA P K+VIP+ +Q+W G PH+ G+FR LWR+G W+E+VIDDRLP+ G+L+F + FW AL+EKAYAK+ GSYE+L GG T E+++DF+GGI E I L + S+ E + LFK L SL+ A I S + +E+ LA+GL+ HAYS+T V + ++S + ++ + L +RL+NPWG EWNG+ SD SEEW IS ++ MG V +DG+FWM D +F ++T+ R + T Y ++ + V D+ AL + Q + Q+ G +G + VE NR F +H Y SRS+ LR L G Y++ P T SP L +LLR YT + ++ L ++P
Sbjct: 1 MFFSTKMVVSYRDQNYGRLKDGCRQEGRLFEDPAFPADSRSLYYS--RTPPEGVEWKRPQELVQNPRLFAEGGISTHDLHQGSLGNCWFVAAAACLAGDPSVWKRVIPSPKEQDWPRPGAPPHA--GIFRFRLWRFGSWIEIVIDDRLPTVRGELLFCRPGQRGAFWCALLEKAYAKLNGSYEALDGGSTAEALIDFSGGISEPIDLLGENFTSEEERKKLFKALLKAHSRASLISAAIRPTSGQSLEQVLASGLVIGHAYSVTSVRSITLRSG-LLSLFRTHKLRLVRLQNPWGSGEWNGAWSDGSEEWKRISRAEREKMGVTVSDDGDFWMSWGDFCTHFTDLTLCRRVNTSTHYTLTCPRAPIILLPAPIPTLYLPAPIPTLYLPFSFDVTLDQDVALISVEQEDR----------------RTQRSEGGENLPIGFFVFKVEVNRKFRLHRTATKVSSSTYI--NSRSVFLRTELQRGRYVILP--TTFSPGLIG--SFLLRLYTDRASHLRELTRDIP 496
BLAST of Calpain 5a vs. Ensembl Xenopus
Match: ppa2 (pyrophosphatase (inorganic) 2 [Source:Xenbase;Acc:XB-GENE-961143]) HSP 1 Score: 271.937 bits (694), Expect = 1.245e-80 Identity = 191/594 (32.15%), Postives = 284/594 (47.81%), Query Frame = 2
Query: 68 FNNQNYVSMRQEHQKNLTLFCDDTFGADDKSISPSGKQEILSSIEWKRPSELCINPCLFIAGSSVHDVCQGSLGNCWLVSALAVLAQHPECLKKVIPNVDDQEWHGNRPHSYTGMFRICLWRYGEWLEVVIDDRLPSRNGKLVFVHSKVKNEFWSALIEKAYAKVCGSYESLVGGLTCESMVDFTGGIPEQIFDLKK--YSKSDTEMENLFKLLHFDWKDGSLM-CATIASRSDEMEEKLANGLITTHAYSITGVCKLRIKSTPMYEHYQKNHLMCIRLRNPWGQKEWNGSLSDASEEWHSISHKDKIAMGWKVENDGEFWMLIDDVLVNFNEITIIRPIKTGIFKYNSNWFGSMCKGYWTGVAGDRQSALAEIG---------QGGQFKIEINNPKGDIFLC------HLLQKCARGSEAALGILIIAVERNRDFMVHTPKFCNVKLEYRLGGSRSICLRYRLTCGTYIVKPFLTVASPSLKFPIYYLLRCYTTQKNNGTFLPSEMPILERNCICKSNCTIIIC----RLHXXXXXXXXXXEELDVLNIFCEIDCENQSLRTTVNCNTSGLFWDETFLLHRSKMDRPIIIKIF 1783
QNY +++E KN LF D F A D+S+ S ++ IEWKRP ELC +P LF+ G S HD+ QG LGNCW V+A + LA KVI N QEW RPH Y G+F W G+W++VVIDDRLPS +G LV+ HS VKNEFWSAL+EKAYAK+ GSYE+L GG T +++VDFTG + E F+L K Y S E LF+ L ++ G L+ C S++ME + GL+ HAYS+T + K+ P GQ+ L W+ +S +K +G V +DGEFWM +D ++F + R + T W G W+ + L G Q Q+ E+ + ++ + + +K +G+ +G I VE NR++ +H L R+I LR LT G Y++ P T P + ++LR +T + + L + P K +C ++C R+ + ++ + + I E +++R+ V TS +D + R + PI I+++
Sbjct: 8 LKTQNYHELKKECIKNGKLFEDPEFPASDESLFYSKAPDL--RIEWKRPKELCEDPHLFVDGISSHDLHQGRLGNCWFVAACSCLALKKALWTKVISNWKKQEWDPKRPHRYAGIFHFKFWCLGDWVDVVIDDRLPSVDGNLVYCHSNVKNEFWSALLEKAYAKLAGSYEALDGGNTADAIVDFTGAVTES-FELDKGDYRTSLMEQTKLFQELLKVYRAGGLISCYIKPVSSNDMEAVTSYGLVKGHAYSVTAIKKM-----------------------PVGQR----ILCFGMTSWNKVSRSEKATLGLTVADDGEFWMTFEDWCLSFTHADVCRILNTSYLSCYKTWKQEEVHGAWS---KNTDPLLNRSGGCFNETTFFQNPQYIFEVRKKEDEVLISLQQKDQRIHKKDGKGNNFIIGFEIFKVELNREYRIHKISGQEKTLRSSYINLRAIFLRKVLTHGRYVIIP--TTFHPGISSS--FILRLFTDESSRFRELLLDKP--------KVSCWSLLCGYPQRVTQVLVRHVEGVGRQGQIQLHV-IKGEKKTIRSLVYKKTSTAKFDIQAIFFRKNISEPICIQVW 555
BLAST of Calpain 5a vs. Ensembl Xenopus
Match: CAPN3 (calpain 3 [Source:NCBI gene;Acc:100497532]) HSP 1 Score: 269.626 bits (688), Expect = 2.307e-79 Identity = 168/508 (33.07%), Postives = 271/508 (53.35%), Query Frame = 2
Query: 62 VFFNNQNYVSMRQEHQKNLTLFCDDTFGADDKSISP---SGKQEILSSIEWKRPSELCINPCLFIAGSSVHDVCQGSLGNCWLVSALAVLAQHPECLKKVIPNVDDQEWHGNRPHSYTGMFRICLWRYGEWLEVVIDDRLPSRNGKLVFVHSKVKNEFWSALIEKAYAKVCGSYESLVGGLTCESMVDFTGGIPEQIFDLKKYSKSDTEMENLFKLLHFDWKDGSLMCATIASRSD-EMEEKLANGLITTHAYSITGVCKLRIKSTPMYEHYQKNHLMCIRLRNPWGQKEWNGSLSDASEEWHSISHKDKIAMGWKVENDGEFWMLIDDVLVNFNEITIIRPIKTGIFKYNSN-WFGSMCKGYWTG--VAGDRQSALAEIGQGGQFKIEI----NNPKGDIFLCH----LLQKCARGSEAA------LGILIIAVERN-----RDFMVHTPKFCNVKLEYRLGGSRSICLRYRLTCGTYIVKPFLTVASPSLKFPIYYLLRCYTTQKN 1507
V F NQ++ +++ E LF D F +S+ + K L +I WKRP E+C NP + G++ D+CQG LG+CW ++A+A L + + L +VIP DQ + N Y G+F WRYG+W++V+IDD LP+ N +LVF S +NEFWSAL+EKAYAK+ GSYE+L GG T E+M DFTGG+ E ++LK+ K +++ ++ ++ GSL+ +I + ++E ++ANGL+ HAYS+TGV + ++K P + +RLRNPWGQ EWNG+ D ++EW + +K + +V+ DGEFWM DD + NF + I + + W S+ +G W AG ++ Q+++++ ++P + +C L+QK R A +G I + N +DF ++ K + R +C R+RL Y++ P + P + ++LR ++ ++N
Sbjct: 30 VKFENQDFATLKSEFVGKKKLFEDPIFLPAVQSLGQKEFAQKSTKLKNIIWKRPKEICENPQFILGGANRTDICQGDLGDCWFLAAIACLTLNEKVLFRVIP--PDQNFTDN----YAGIFHFQFWRYGDWVDVIIDDYLPTYNNELVFTKSSQRNEFWSALLEKAYAKLHGSYEALKGGNTTEAMEDFTGGVTE-FYELKEAPK------DMYNIMKKAFERGSLIGCSIDTLVPVQLETRMANGLVKGHAYSVTGVEETKLKGKP---------IKLVRLRNPWGQVEWNGAWGDNAKEWTMVDKSEKTRLQHQVQEDGEFWMSYDDFMKNFTKAEICNLTPDALDSDSLQVWTVSVNEGRWVRGCSAGGCRNYPDTYWTNPQYRLKLLEEDDDPADNEVVCSFVVALMQKNRRKDRKAGANLFTIGFAIYEMHGNNQHLQKDFFLYNASKAKCKSYINM---REVCQRFRLPPSEYVIIP--STYEPHQEGE--FILRVFSEKRN 508
BLAST of Calpain 5a vs. Ensembl Xenopus
Match: icmt (isoprenylcysteine carboxyl methyltransferase [Source:Xenbase;Acc:XB-GENE-976857]) HSP 1 Score: 262.692 bits (670), Expect = 6.989e-78 Identity = 171/540 (31.67%), Postives = 280/540 (51.85%), Query Frame = 2
Query: 329 HPECLK--KVIPNVDDQEWHGNRPHSYTGMFRICLWRYGEWLEVVIDDRLPSRNGKLVFVHSKVKNEFWSALIEKAYAKVCGSYESLVGGLTCESMVDFTGGIPEQIFDLKKYSKSDTEMEN-LF-KLLHFDWKDGSLMCATIASRSDEMEEKLANGLITTHAYSITGVCKLRIKSTPMYEHYQKNHLMCIRLRNPWGQKEWNGSLSDASEEWHSISHKDKIAMGWKVENDGEFWMLIDDVLVNFNEITIIRPIKTGIFKYNSNWFGSMCKGYWTGVAGDRQSA-------LAEIGQGGQFKIEINNPKGDIFLCHLLQKCARGSEA-------ALGILIIAVERNRDFMVHT--PKFCNVKLEYRLGGSRSICLRYRLTCGTYIVKPFLTVASPSLKFPIYYLLRCYTTQKNNGTFLPS---EMPILERNCICKSNC---TIIICRLHXXXXXXXXXXEELDVLNIFCEIDCENQSLRTTVNCNTSGLFWDETFLLHRSKMDRPIIIKIFHRGRFFNTLIGYSCCSYDSRHLNSPEVIE 1870
HP L VIP+ +QEW+ ++P Y G+F W++GEW++VVIDDRLP+ N +L++ HS KNE W AL+EKAYAK+ G YE+L GG T +++VDFTGG+ E I + + +D E N LF ++L + G + C+ A+ + +ME ++A GL+ HAY++T V K+R+ + ++ L IR+RNPWG++EWNG SD+SEEW +S ++ +G VE+DGEFWM +D + +I + R I T + W ++ + W Q+ A Q QF ++ P+ ++ +C L QK R + A+G I VE NR F +HT PK + SR + LR L G Y++ P T P +LLR +T LPS E+ + E C S ++ ++H++ A ++ + + + I CE +++ V + ++ + +R K +PI++++++ + +G S D+ +L + +E
Sbjct: 4 HPIALSFIVVIPDWKEQEWYADKPQIYAGIFHFHFWKFGEWVDVVIDDRLPTINNQLIYCHSNEKNELWCALVEKAYAKLAGCYEALDGGNTADALVDFTGGVSEPIELTEGHFVTDVEKRNDLFERVLKVHNRGGLISCSIRATSASDMEARMACGLVKGHAYAVTDVRKVRL-GHGLLAFFKSEKLEMIRMRNPWGEREWNGPWSDSSEEWQKVSKSEREKLGVTVEDDGEFWMTFEDFCKYYTDIIMCRLINTSYLSIHKTWEEAVLRSSWVQHQDPLQNRSGGCLNFKATYLQNPQFVFDVKKPEDEVLVC-LQQKTKRTTRQEGKAENLAIGFEIHQVELNRRFRMHTLQPKVASSIYI----NSRCVFLRTDLKEGRYVIIP--TTFDPGHTGE--FLLRVFTD-------LPSNCCELKLDEPTKTCWSGLCGYPQLVSQVHVVSAQGLKQQDSNGGADPYVIISCEGNKVQSPVIKDCLDPKFNVKGVFYRKKPGQPIVVQVWNHNVIKDEFMGLVDLSGDANNLQTQHSLE 526
BLAST of Calpain 5a vs. Ensembl Mouse
Match: Capn5 (calpain 5 [Source:MGI Symbol;Acc:MGI:1100859]) HSP 1 Score: 328.561 bits (841), Expect = 8.812e-102 Identity = 196/599 (32.72%), Postives = 311/599 (51.92%), Query Frame = 2
Query: 68 FNNQNYVSMRQEHQKNLTLFCDDTFGADDKSISPSGKQEILSSIEWKRPSELCINPCLFIAGSSVHDVCQGSLGNCWLVSALAVLAQHPECLKKVIPNVDDQEWHGNRPHSYTGMFRICLWRYGEWLEVVIDDRLPSRNGKLVFVHSKVKNEFWSALIEKAYAKVCGSYESLVGGLTCESMVDFTGGIPEQIFDLKK--YSKSDTEMENLFKLLHFDWKDGSLMCATI-ASRSDEMEEKLANGLITTHAYSITGVCKLRIKSTPMYEHYQKNHLMCIRLRNPWGQKEWNGSLSDASEEWHSISHKDKIAMGWKVENDGEFWMLIDDVLVNFNEITIIRPIKTGIFKYNSNWFGSMCKGYWTGVAGDRQSALA-------EIGQGGQFKIEINNPKGDIFLC------HLLQKCARGSEAALGILIIAVERNRDFMVHTPKFCNVKLEYRLGGSRSICLRYRLTCGTYIVKPFLTVASPSLKFPIYYLLRCYTTQKNNGTFLPSEMPILERNCICKSNCTIIICRLHXXXXXXXXXXEELDVLNIFCEIDCENQSLRTTVNCNTSGLFWDETFLLHRSKMDRPIIIKIFHRGRFFNTLIG 1816
+ +QNY ++++ + LF D F A D S+ G ++ WKRP ++C +P LF+ G S HD+ QG +GNCW V+A + LA +KVIP+ +QEW+ +P SY G+F WR+GEW++V++DDRLP+ N +L++ HS KNEFW AL+EKAYAK+ G Y++L GG T +++VDFTGG+ E I DL + + + + LF+ + G L+ A+I A + +ME +LA GL+ HAY++T V K+R+ + ++ L IRLRNPWG++EW G SD SEEW +S ++ MG V++DGEFWM +D+ F +I R I T + W + G WT +Q+ Q Q+ E+ P+ ++ + ++ +G A+G I VE NR + +H+ + Y SRS+ LR L G Y++ P + +F LLR +T +N L + P R C S C + + N + I CE + +R+ V TS ++ + +R K+ +PI +++++ + +G
Sbjct: 8 YEDQNYSALKRACLRKKVLFEDPLFPATDDSLYYKGTPG--PTVRWKRPKDICDDPRLFVDGISSHDLHQGQVGNCWFVAACSSLASRESLWQKVIPDWKEQEWNPEKPDSYAGIFHFNFWRFGEWVDVIVDDRLPTVNNQLIYCHSNSKNEFWCALVEKAYAKLAGCYQALDGGNTADALVDFTGGVSEPI-DLTEGDLATDEAKRNQLFERVLKVHSRGGLISASIKAVTAADMEARLACGLVKGHAYAVTDVRKVRLGHG-LLAFFKSEKLDMIRLRNPWGEREWTGPWSDTSEEWQKVSKSEREKMGVTVQDDGEFWMTFEDMCRYFTDIIKCRLINTSYLSIHKTWEEARLHGAWTRHEDPQQNRSGGCINHKDTFFQNPQYVFEVKKPEDEVLISIQQRPKRSTRREGKGENLAIGFDIYKVEENRQYRMHSLQHKAASSIYI--NSRSVFLRTELPEGRYVIIPTTFEPGHTGEF----LLRVFTDVPSNCRELRLDEP--PRTC-WSSLCGYPQQVAQVHVLGAAGLKDSPTGANSYVIIKCEGEKVRSAVQRGTSTPEYNVKGIFYRKKLAQPITVQVWNHRVLKDEFLG 593
BLAST of Calpain 5a vs. Ensembl Mouse
Match: Capn5 (calpain 5 [Source:MGI Symbol;Acc:MGI:1100859]) HSP 1 Score: 328.561 bits (841), Expect = 8.812e-102 Identity = 196/599 (32.72%), Postives = 311/599 (51.92%), Query Frame = 2
Query: 68 FNNQNYVSMRQEHQKNLTLFCDDTFGADDKSISPSGKQEILSSIEWKRPSELCINPCLFIAGSSVHDVCQGSLGNCWLVSALAVLAQHPECLKKVIPNVDDQEWHGNRPHSYTGMFRICLWRYGEWLEVVIDDRLPSRNGKLVFVHSKVKNEFWSALIEKAYAKVCGSYESLVGGLTCESMVDFTGGIPEQIFDLKK--YSKSDTEMENLFKLLHFDWKDGSLMCATI-ASRSDEMEEKLANGLITTHAYSITGVCKLRIKSTPMYEHYQKNHLMCIRLRNPWGQKEWNGSLSDASEEWHSISHKDKIAMGWKVENDGEFWMLIDDVLVNFNEITIIRPIKTGIFKYNSNWFGSMCKGYWTGVAGDRQSALA-------EIGQGGQFKIEINNPKGDIFLC------HLLQKCARGSEAALGILIIAVERNRDFMVHTPKFCNVKLEYRLGGSRSICLRYRLTCGTYIVKPFLTVASPSLKFPIYYLLRCYTTQKNNGTFLPSEMPILERNCICKSNCTIIICRLHXXXXXXXXXXEELDVLNIFCEIDCENQSLRTTVNCNTSGLFWDETFLLHRSKMDRPIIIKIFHRGRFFNTLIG 1816
+ +QNY ++++ + LF D F A D S+ G ++ WKRP ++C +P LF+ G S HD+ QG +GNCW V+A + LA +KVIP+ +QEW+ +P SY G+F WR+GEW++V++DDRLP+ N +L++ HS KNEFW AL+EKAYAK+ G Y++L GG T +++VDFTGG+ E I DL + + + + LF+ + G L+ A+I A + +ME +LA GL+ HAY++T V K+R+ + ++ L IRLRNPWG++EW G SD SEEW +S ++ MG V++DGEFWM +D+ F +I R I T + W + G WT +Q+ Q Q+ E+ P+ ++ + ++ +G A+G I VE NR + +H+ + Y SRS+ LR L G Y++ P + +F LLR +T +N L + P R C S C + + N + I CE + +R+ V TS ++ + +R K+ +PI +++++ + +G
Sbjct: 8 YEDQNYSALKRACLRKKVLFEDPLFPATDDSLYYKGTPG--PTVRWKRPKDICDDPRLFVDGISSHDLHQGQVGNCWFVAACSSLASRESLWQKVIPDWKEQEWNPEKPDSYAGIFHFNFWRFGEWVDVIVDDRLPTVNNQLIYCHSNSKNEFWCALVEKAYAKLAGCYQALDGGNTADALVDFTGGVSEPI-DLTEGDLATDEAKRNQLFERVLKVHSRGGLISASIKAVTAADMEARLACGLVKGHAYAVTDVRKVRLGHG-LLAFFKSEKLDMIRLRNPWGEREWTGPWSDTSEEWQKVSKSEREKMGVTVQDDGEFWMTFEDMCRYFTDIIKCRLINTSYLSIHKTWEEARLHGAWTRHEDPQQNRSGGCINHKDTFFQNPQYVFEVKKPEDEVLISIQQRPKRSTRREGKGENLAIGFDIYKVEENRQYRMHSLQHKAASSIYI--NSRSVFLRTELPEGRYVIIPTTFEPGHTGEF----LLRVFTDVPSNCRELRLDEP--PRTC-WSSLCGYPQQVAQVHVLGAAGLKDSPTGANSYVIIKCEGEKVRSAVQRGTSTPEYNVKGIFYRKKLAQPITVQVWNHRVLKDEFLG 593
BLAST of Calpain 5a vs. Ensembl Mouse
Match: Capn6 (calpain 6 [Source:MGI Symbol;Acc:MGI:1100850]) HSP 1 Score: 256.144 bits (653), Expect = 1.044e-74 Identity = 167/516 (32.36%), Postives = 259/516 (50.19%), Query Frame = 2
Query: 65 FFNNQNYVSMRQEHQKNLTLFCDDTFGADDKSISPSGKQEILSSIEWKRPSELCINPCLFIAGSSVHDVCQGSLGNCWLVSALAVLAQHPECLKKVIPNVDDQEWHGNRPHSYTGMFRICLWRYGEWLEVVIDDRLPSRNGKLVFVHSKVKNEFWSALIEKAYAKVCGSYESLVGGLTCESMVDFTGGIPEQIFDLKK--YSKSDTEMENLFKLLHFDWKDGSLMCATIASRSDEMEEKLAN-GLITTHAYSITGVCKLRIKSTPMYEHYQKNHLMCIRLRNPWGQKEWNGSLSDASEEWHSISHKDKIAMGWKVENDGEFWMLIDDVLVNFNEITIIRPIKTGIFKYNSNWFGSMCKGYWT-----------GVAGDRQSALAEIGQGGQFKIEINNPKGDIFLC------HLLQKCARGSEAALGILIIAVERNRDFMVHTPKFCNVKLEYRLGGS-----RSICLRYRLTCGTYIVKPFLTVASPSLKFPIYYLLRCYTTQKNNGTFLPSEMP 1537
F NQ Y ++QE K+ LFCD TF ++ S+ + + + + WKRP ++ +P L + S H + QG LGN ++SA + LA K IPN DQEW +P Y G+F W +GEW EVVIDD LP+ NG LVF S NEFW+AL+EKAYAK+ G YE+L G + ++DFTG + E I D++K Y+ E LF L+ + G L+C +I S S E +E + GL+ + Y++T + KLR+ + E + L +RLRNP G++EW+G S+ SEEW ++ D+ +G + +DGEFWM ++D NF+++ + R + +F G WT G +R + L Q Q+ + + + ++ R +G + VE NR F +H ++ ++ R G S R++ L L G+Y++ P + + +F LLR ++ L +MP
Sbjct: 7 LFKNQKYQELKQECMKDGRLFCDPTFLPENDSLFFN--RLLPGKVVWKRPQDISDDPHLIVGNISNHQLIQGRLGNKAMISAFSCLAVQESHWTKAIPNHKDQEWDPRKPEKYAGIFHFRFWHFGEWTEVVIDDLLPTINGDLVFSFSTSMNEFWNALLEKAYAKLLGCYEALDGLTITDIIMDFTGTLAE-IIDMQKGRYTDLVEEKYKLFGELYKTFTKGGLICCSIESPSQEEQEVETDWGLLKGYTYTMTDIRKLRLGER-LVEVFSTEKLYMVRLRNPLGRQEWSGPWSEISEEWQQLTVTDRKNLGLVMSDDGEFWMSLEDFCHNFHKLNVCRNVNNPVFGRKE---LESVVGCWTVDDDPLMNRSGGCYNNRDTFL----QNPQYIFTVPEDGHKVIMSLQQKDLRTYRRMGRPDNYIIGFELFKVEMNRRFRLH-----HLYIQERAGTSTYIDTRTVFLSKYLKKGSYVLVPTMFQHGRTSEF----LLRIFSEVPVQLRELTLDMP 502
BLAST of Calpain 5a vs. Ensembl Mouse
Match: Capn8 (calpain 8 [Source:MGI Symbol;Acc:MGI:2181366]) HSP 1 Score: 248.44 bits (633), Expect = 1.107e-74 Identity = 139/345 (40.29%), Postives = 201/345 (58.26%), Query Frame = 2
Query: 50 SRNFVFFNNQNYVSMRQEHQKNLTLFCDDTFGA-----DDKSISPSGKQEILSSIEWKRPSELCINPCLFIAGSSVHDVCQGSLGNCWLVSALAVLAQHPECLKKVIPNVDDQEWHGNRPHSYTGMFRICLWRYGEWLEVVIDDRLPSRNGKLVFVHSKVKNEFWSALIEKAYAKVCGSYESLVGGLTCESMVDFTGGIPEQIFDLKKYSKSDTEMENLFKLLHFDWKDGSLM-CATIASRSDEMEEKLANGLITTHAYSITGVCKLRIKSTPMYEHYQKNHLMCIRLRNPWGQKEWNGSLSDASEEWHSISHKDKIAMGWKVENDGEFWMLIDDVLVNFNEITI 1066
++N V + Q++ ++R++ + LF D F A + + P + I WKRP+ELC NP + G++ D+ QG LG+CWL++A+A L + + L +V+P DQ + N Y G+F W+YGEW+EVVIDDRLP++NG+L+F+HS+ NEFWSAL+EKAYAK+ GSYE+L GG T E DFTGGI E +DL+K NL+ + + GSL+ C+ S + E E L+ HAYS+TGV ++ + P IRLRNPWG+ EW G+ SD++ EW+ I + K + + E DGEFWM D L F+ + I
Sbjct: 21 NQNAVKYLGQDFETLRKQCLNSGVLFKDPEFPACPSALGYRDLGPGSAE--TQGIIWKRPTELCSNPQFIVGGATRTDIRQGGLGDCWLLAAIASLTLNEKLLYRVVPR--DQSFQKN----YAGIFHFQFWQYGEWVEVVIDDRLPTKNGQLLFLHSEEGNEFWSALLEKAYAKLNGSYEALAGGSTIEGFEDFTGGISE-FYDLRKPPG------NLYYTIQKALRKGSLLGCSIDVSNAAEAEATTRQKLVKGHAYSVTGVEEVDFRGLPE---------KLIRLRNPWGEVEWTGAWSDSAPEWNYIDPQKKGELDKRAE-DGEFWMSFSDFLKQFSRLEI 340
BLAST of Calpain 5a vs. Ensembl Mouse
Match: Capn11 (calpain 11 [Source:MGI Symbol;Acc:MGI:1352490]) HSP 1 Score: 254.218 bits (648), Expect = 2.881e-73 Identity = 177/525 (33.71%), Postives = 266/525 (50.67%), Query Frame = 2
Query: 53 RNFVFFNNQNYVSMRQEHQKNLTLFCDDTFGADDKSISPSG---KQEILSSIEWKRPSELCINPCLFIAGSSVHDVCQGSLGNCWLVSALAVLAQHPECLKKVIPNVDDQEWHGNRPHSYTGMFRICLWRYGEWLEVVIDDRLPSRNGKLVFVHSKVKNEFWSALIEKAYAKVCGSYESLVGGLTCESMVDFTGGIPEQIFDLKKYSKSDTEMENLFKLLHFDWKDGSLM-CATIASRSDEMEEKLANGLITTHAYSITGVCKLRIKSTPMYEHYQKNHLMCIRLRNPWGQKEWNGSLSDASEEWHSISHKDKIAMGWKVENDGEFWMLIDDVLVNFN--EITIIRPIKTGIFKYNSNWFGSMCKGYWT--GVAGDRQSALAEIGQGGQFKI---EINNPKGD--------IFLCHLLQKCARGSEAA-----LGILIIAVERN----RDFMVHTPKFCNVKLEYRLGG-------SRSICLRYRLTCGTYIVKPFLTVASPSLKFPIYYLLRCYTTQKNNGTFL 1522
+N + NQNY +R E + LF D F A+ +SI E + +I W+RP ++ NP S D+CQG LG+CWL++A+ L P+ L +V+P +Q + N Y G+F LW++G WL VV+DDRLP+RN KLVFVH+ + +FWSAL+EKAYAK+ GSY +L GG T E + DFTGG+ Q L+K N+ +LL + SLM C+ + + E+E N L+ HAY++TG+ + +Y+ IR++NPWG+ EWNG+ SD + EW +S ++ + K ++DG+FWM +D + NF EI + P + Y S W + +G W AG ++ QFKI E+++P+ D L L+QK R + +G +I +V + RD + F L+YR G SR + RL G Y++ P F LLR + T+K++ T+L
Sbjct: 32 QNAYNYKNQNYEDLRAECLRKGELFEDPFFPAEPRSIGVKNLGPNSEHMQNIYWQRPKDIIHNPQFITNDFSPTDICQGILGDCWLLAAIGSLTTCPKLLYRVVPR--NQSFKKN----YAGIFHFQLWQFGHWLNVVVDDRLPTRNNKLVFVHASHRQDFWSALLEKAYAKLIGSYGALSGGSTLEGLEDFTGGVA-QCIPLQKPPG------NMLRLLKKALEKSSLMGCSIEVTDNSEVETMTHNMLVRGHAYAVTGLEDV---------YYRDKLETLIRIQNPWGRVEWNGAWSDKATEWEEVSPDVRVQLLHK-KDDGDFWMSYEDFMSNFTLLEICNLTPDALNTWDYKSRWHSTFYEGSWRRGSTAGGCRNHPETFWSNPQFKISLPEVDDPEDDSEKNEMVCTCLVALMQKNWRHAREGPQLLTIGFVIFSVPKEFQNLRDIHLKKDFF----LKYRDHGFSEIFINSREVNSHLRLPPGEYVIIPSTYEPHKDADF----LLRVF-TEKHSETWL 524
BLAST of Calpain 5a vs. UniProt/SwissProt
Match: sp|O15484|CAN5_HUMAN (Calpain-5 OS=Homo sapiens OX=9606 GN=CAPN5 PE=1 SV=2) HSP 1 Score: 336.265 bits (861), Expect = 7.390e-104 Identity = 204/622 (32.80%), Postives = 324/622 (52.09%), Query Frame = 2
Query: 68 FNNQNYVSMRQEHQKNLTLFCDDTFGADDKSISPSGKQEILSSIEWKRPSELCINPCLFIAGSSVHDVCQGSLGNCWLVSALAVLAQHPECLKKVIPNVDDQEWHGNRPHSYTGMFRICLWRYGEWLEVVIDDRLPSRNGKLVFVHSKVKNEFWSALIEKAYAKVCGSYESLVGGLTCESMVDFTGGIPEQIFDLKK--YSKSDTEMENLFKLLHFDWKDGSLMCATI-ASRSDEMEEKLANGLITTHAYSITGVCKLRIKSTPMYEHYQKNHLMCIRLRNPWGQKEWNGSLSDASEEWHSISHKDKIAMGWKVENDGEFWMLIDDVLVNFNEITIIRPIKTGIFKYNSNWFGSMCKGYWTGVAGDRQSALA-------EIGQGGQFKIEINNPKGDIFLC------HLLQKCARGSEAALGILIIAVERNRDFMVHTPKFCNVKLEYRLGGSRSICLRYRLTCGTYIVKPFLTVASPSLKFPIYYLLRCYTTQKNNGTFLPSEMPILERNCICKSN-CTIIICRLHXXXXXXXXXXEELDVLNIFCEIDCENQSLRTTVNCNTSGLFWDETFLLHRSKMDRPIIIKIFHRGRFFNTLIGYSCCSYDSRHLNSPEVIETLNM 1882
+ +QNY ++R++ ++ LF D F A D S+ G ++ WKRP +C +P LF+ G S HD+ QG +GNCW V+A + LA +KVIP+ +QEW +P++Y G+F WR+GEW++VVIDDRLP+ N +L++ HS +NEFW AL+EKAYAK+ G Y++L GG T +++VDFTGG+ E I DL + ++ +T+ LF+ + G L+ A+I A + +ME +LA GL+ HAY++T V K+R+ + ++ L IRLRNPWG++EWNG SD SEEW +S ++ MG V++DGEFWM +DV F +I R I T + W + G WT RQ+ Q Q+ E+ P+ ++ +C ++ +G A+G I VE NR + +H+ + Y SRS+ LR G Y++ P + +F LLR +T +N E+ + E C S+ C + + N + I CE +R+ V TS ++ + +R K+ +PI +++++ + +G D +L + + TL++
Sbjct: 8 YEDQNYSALRRDCRRRKVLFEDPLFPATDDSLYYKGTPG--PAVRWKRPKGICEDPRLFVDGISSHDLHQGQVGNCWFVAACSSLASRESLWQKVIPDWKEQEWDPEKPNAYAGIFHFHFWRFGEWVDVVIDDRLPTVNNQLIYCHSNSRNEFWCALVEKAYAKLAGCYQALDGGNTADALVDFTGGVSEPI-DLTEGDFANDETKRNQLFERMLKVHSRGGLISASIKAVTAADMEARLACGLVKGHAYAVTDVRKVRLGHG-LLAFFKSEKLDMIRLRNPWGEREWNGPWSDTSEEWQKVSKSEREKMGVTVQDDGEFWMTFEDVCRYFTDIIKCRVINTSHLSIHKTWEEARLHGAWTLHEDPRQNRGGGCINHKDTFFQNPQYIFEVKKPEDEVLICIQQRPKRSTRREGKGENLAIGFDIYKVEENRQYRMHSLQHKAASSIYI--NSRSVFLRTDQPEGRYVIIPTTFEPGHTGEF----LLRVFTDVPSNC----RELRLDEPPHTCWSSLCGYPQLVTQVHVLGAAGLKDSPTGANSYVIIKCEGDKVRSAVQKGTSTPEYNVKGIFYRKKLSQPITVQVWNHRVLKDEFLGQVHLKADPDNL---QALHTLHL 612
BLAST of Calpain 5a vs. UniProt/SwissProt
Match: sp|O08688|CAN5_MOUSE (Calpain-5 OS=Mus musculus OX=10090 GN=Capn5 PE=1 SV=1) HSP 1 Score: 328.561 bits (841), Expect = 6.164e-101 Identity = 196/599 (32.72%), Postives = 311/599 (51.92%), Query Frame = 2
Query: 68 FNNQNYVSMRQEHQKNLTLFCDDTFGADDKSISPSGKQEILSSIEWKRPSELCINPCLFIAGSSVHDVCQGSLGNCWLVSALAVLAQHPECLKKVIPNVDDQEWHGNRPHSYTGMFRICLWRYGEWLEVVIDDRLPSRNGKLVFVHSKVKNEFWSALIEKAYAKVCGSYESLVGGLTCESMVDFTGGIPEQIFDLKK--YSKSDTEMENLFKLLHFDWKDGSLMCATI-ASRSDEMEEKLANGLITTHAYSITGVCKLRIKSTPMYEHYQKNHLMCIRLRNPWGQKEWNGSLSDASEEWHSISHKDKIAMGWKVENDGEFWMLIDDVLVNFNEITIIRPIKTGIFKYNSNWFGSMCKGYWTGVAGDRQSALA-------EIGQGGQFKIEINNPKGDIFLC------HLLQKCARGSEAALGILIIAVERNRDFMVHTPKFCNVKLEYRLGGSRSICLRYRLTCGTYIVKPFLTVASPSLKFPIYYLLRCYTTQKNNGTFLPSEMPILERNCICKSNCTIIICRLHXXXXXXXXXXEELDVLNIFCEIDCENQSLRTTVNCNTSGLFWDETFLLHRSKMDRPIIIKIFHRGRFFNTLIG 1816
+ +QNY ++++ + LF D F A D S+ G ++ WKRP ++C +P LF+ G S HD+ QG +GNCW V+A + LA +KVIP+ +QEW+ +P SY G+F WR+GEW++V++DDRLP+ N +L++ HS KNEFW AL+EKAYAK+ G Y++L GG T +++VDFTGG+ E I DL + + + + LF+ + G L+ A+I A + +ME +LA GL+ HAY++T V K+R+ + ++ L IRLRNPWG++EW G SD SEEW +S ++ MG V++DGEFWM +D+ F +I R I T + W + G WT +Q+ Q Q+ E+ P+ ++ + ++ +G A+G I VE NR + +H+ + Y SRS+ LR L G Y++ P + +F LLR +T +N L + P R C S C + + N + I CE + +R+ V TS ++ + +R K+ +PI +++++ + +G
Sbjct: 8 YEDQNYSALKRACLRKKVLFEDPLFPATDDSLYYKGTPG--PTVRWKRPKDICDDPRLFVDGISSHDLHQGQVGNCWFVAACSSLASRESLWQKVIPDWKEQEWNPEKPDSYAGIFHFNFWRFGEWVDVIVDDRLPTVNNQLIYCHSNSKNEFWCALVEKAYAKLAGCYQALDGGNTADALVDFTGGVSEPI-DLTEGDLATDEAKRNQLFERVLKVHSRGGLISASIKAVTAADMEARLACGLVKGHAYAVTDVRKVRLGHG-LLAFFKSEKLDMIRLRNPWGEREWTGPWSDTSEEWQKVSKSEREKMGVTVQDDGEFWMTFEDMCRYFTDIIKCRLINTSYLSIHKTWEEARLHGAWTRHEDPQQNRSGGCINHKDTFFQNPQYVFEVKKPEDEVLISIQQRPKRSTRREGKGENLAIGFDIYKVEENRQYRMHSLQHKAASSIYI--NSRSVFLRTELPEGRYVIIPTTFEPGHTGEF----LLRVFTDVPSNCRELRLDEP--PRTC-WSSLCGYPQQVAQVHVLGAAGLKDSPTGANSYVIIKCEGEKVRSAVQRGTSTPEYNVKGIFYRKKLAQPITVQVWNHRVLKDEFLG 593
BLAST of Calpain 5a vs. UniProt/SwissProt
Match: sp|Q8R4C0|CAN5_RAT (Calpain-5 OS=Rattus norvegicus OX=10116 GN=Capn5 PE=1 SV=1) HSP 1 Score: 324.324 bits (830), Expect = 1.977e-99 Identity = 197/599 (32.89%), Postives = 310/599 (51.75%), Query Frame = 2
Query: 68 FNNQNYVSMRQEHQKNLTLFCDDTFGADDKSISPSGKQEILSSIEWKRPSELCINPCLFIAGSSVHDVCQGSLGNCWLVSALAVLAQHPECLKKVIPNVDDQEWHGNRPHSYTGMFRICLWRYGEWLEVVIDDRLPSRNGKLVFVHSKVKNEFWSALIEKAYAKVCGSYESLVGGLTCESMVDFTGGIPEQIFDLKK--YSKSDTEMENLFKLLHFDWKDGSLMCATI-ASRSDEMEEKLANGLITTHAYSITGVCKLRIKSTPMYEHYQKNHLMCIRLRNPWGQKEWNGSLSDASEEWHSISHKDKIAMGWKVENDGEFWMLIDDVLVNFNEITIIRPIKTGIFKYNSNWFGSMCKGYWTGVAGDRQSALA-------EIGQGGQFKIEINNPKGDIFLC------HLLQKCARGSEAALGILIIAVERNRDFMVHTPKFCNVKLEYRLGGSRSICLRYRLTCGTYIVKPFLTVASPSLKFPIYYLLRCYTTQKNNGTFLPSEMPILERNCICKSNCTIIICRLHXXXXXXXXXXEELDVLNIFCEIDCENQSLRTTVNCNTSGLFWDETFLLHRSKMDRPIIIKIFHRGRFFNTLIG 1816
+ QNY ++++ + LF D F A D S+ G ++ WKRP ++C +P LF+ G S HD+ QG +GNCW V+A + LA +KVIP+ +QEW+ +P SY G+F WR+GEW++VV+DDRLP+ N +L++ HS KNEFW A +EKAYAK+ G Y++L GG T +++VDFTGG+ E I DL + + + + LF+ + G L+ A+I A + +ME +LA GL+ HAY++T V K+R+ + ++ L IRLRNPWG++ W G SD SEEW +S ++ MG V++DGEFWM +D+ F +I R I T + W + +G WT +Q+ Q Q+ E+ P+ ++ +C ++ +G A+G I VE NR + +H+ + Y SRS+ LR L G Y++ P T P + LLR +T +N L + P R C S C + + N + I CE + +R+ V TS ++ + +R K+ +PI +++++ + +G
Sbjct: 8 YEYQNYSALKRACLRRKVLFEDPHFPASDDSLYYKGTPG--PTVRWKRPFDICDDPRLFVDGISSHDLHQGQVGNCWFVAACSSLASRESLWQKVIPDWKEQEWNPEKPDSYAGIFHFNFWRFGEWVDVVVDDRLPTVNNQLIYCHSNSKNEFWCAPVEKAYAKLAGCYQALDGGNTADALVDFTGGVSEPI-DLTEGDLATDEAKRSQLFERVLKVHSRGGLISASIKAMTAADMETRLACGLVKGHAYAVTDVRKVRLGHG-LLAFFKSEKLDMIRLRNPWGERVWTGPWSDTSEEWQKVSKSEREKMGVTVQDDGEFWMTYEDMCRYFTDIIKCRLINTSYLSIHKTWEEARLRGAWTRHEDPQQNRSGGCINHKDTFFQNPQYIFEVKKPEDEVLICIQQRPKRSTRREGKGENLAIGFDIYKVEENRQYRMHSLQHKAASSIYI--NSRSVFLRTELPEGRYVIIP--TTFEPGHTGEL--LLRVFTDVPSNCRELRLDEP--PRTC-WSSLCGYPQQVTQVHVLGAAGLKDSSTGANSYVIIKCEGEKVRSAVQRGTSTPEYNVKGIFYRKKLSQPITVQVWNNRVLKDEFLG 593
BLAST of Calpain 5a vs. UniProt/SwissProt
Match: sp|Q22036|CAN5_CAEEL (Calpain-5 OS=Caenorhabditis elegans OX=6239 GN=tra-3 PE=1 SV=1) HSP 1 Score: 316.62 bits (810), Expect = 2.438e-96 Identity = 204/600 (34.00%), Postives = 314/600 (52.33%), Query Frame = 2
Query: 68 FNNQNYVSMRQEHQKNLTLFCDDTFGADDKSISPSGKQEILSSIEWKRPSELCINPCLFIAGSSVHDVCQGSLGNCWLVSALAVLAQHPECLKKVIPNVDDQEWHGNRPHSYTGMFRICLWRYGEWLEVVIDDRLPSRNGKLVFVHSKVKNEFWSALIEKAYAKVCGSYESLVGGLTCESMVDFTGGIPEQIFDLK--KYSKSDTEMENLFKLLHFDWKDGSLMCATIASRS-DEMEEKLANGLITTHAYSITGVCKLRI-----KSTPMYEHYQKNHLMCIRLRNPWGQKEWNGSLSDASEEWHSISHKDKIAMGWKVEN----DGEFWMLIDDVLVNFNEITIIRPIKTGIFKYNSNWFGSMCKGYWT------GVAGDRQSA----LAEIGQGGQFKIEINNPKGDIFLCHLLQKCARGSE-----AALGILIIAVERNRDFMVHTPKFCNVKLEYRLGGSRSICLRYR-LTCGTYIVKPFLTVASPSLKFPIYYLLRCYTTQKNNGTFLPSEMPILERNCICKSNCTIIICRLHXXXXXXXXXXEELDVLNIFCEIDCENQSLRTTVNCNTSGLFWDETFLLHRSKMDRPIIIKIF 1783
F NQNY +R+ K F D F ++S+ +Q S I WKRP EL +P LF+ G+S +DV QG LGNCW VSA + L + + L +VIP+ DDQEW + H+Y G+FR WR+G+W+EVVIDD LP+R+GKL+F SK NEFWSAL+EKA+AK+ G YE+LVGG +++ D +GG+ E + K K +DTE++ LF L + G+L+ A IA+R+ +E+EE L GL+ HAY+++ VC + + +S + K IRL+NPWG+KEWNG+ SD S EW ++S MG + N DG+FWM + + F +I++ + T +F ++ ++ + WT G DR A Q+ +I +P + + + G + +G+ ++ VE NR + VHT +Y G RS+ L + L G Y++ P T +P K ++LR Y+ + + + L P L CKS ++ +H + + N++ + +S RT + WDE FL H+SK + I+++
Sbjct: 10 FGNQNYEKLRKICIKKKQPFVDTLFPPTNQSLFLEQRQS--SDIVWKRPGELHPDPHLFVEGASPNDVTQGILGNCWFVSACSALTHNFKLLAQVIPDADDQEW--STKHAYAGIFRFRFWRFGKWVEVVIDDLLPTRDGKLLFARSKTPNEFWSALLEKAFAKLYGCYENLVGGHLSDALQDVSGGVAETLHVRKFLKDDPNDTELK-LFNDLKTAFDKGALVVAAIAARTKEEIEESLDCGLVKGHAYAVSAVCTIDVTNPNERSFTSFIMGSKRKQNLIRLQNPWGEKEWNGAWSDDSPEWQNVSASQLSTMGVQPANSDSDDGDFWMPWESFVHYFTDISLCQLFNTSVFSFSRSYDEQIVFSEWTTNGKKSGAPDDRAGGCHNFKATFCNNPQYIFDIPSPNCSVMFALIQNDPSEGLKKREPFVTIGMHVMKVENNRQYRVHTAMHPIAISDYASG--RSVYLHLQSLPRGRYLLIP--TTFAP--KEQTLFMLRVYSDEHIHFSPLTKHAPKLGL-LKCKSAQSVTRLTIHGVDFNSASTGTH----NVYAILKDSRKSFRTKTLSGVKSIQWDEQFLFHKSKNRQQYKIEVW 593
BLAST of Calpain 5a vs. UniProt/SwissProt
Match: sp|Q11002|CANA_DROME (Calpain-A OS=Drosophila melanogaster OX=7227 GN=CalpA PE=1 SV=2) HSP 1 Score: 265.774 bits (678), Expect = 8.378e-76 Identity = 181/505 (35.84%), Postives = 268/505 (53.07%), Query Frame = 2
Query: 77 QNYVSMRQEHQKNLTLFCDDTFGADDKSISPSGKQEILSSIEWKRPSELCINPCLFIAGSSVHDVCQGSLGNCWLVSALAVLAQHPECLKKVIPNVDDQEWHGNRPHSYTGMFRICLWRYGEWLEVVIDDRLPSRNGKLVFVHSKVKNEFWSALIEKAYAKVCGSYESLVGGLTCESMVDFTGGIPEQIFDLKKYSKSDTEMENLFKLLHFDWKDGSLMCATIASRSDEMEEKLANGLITTHAYSITGVCKLRIKSTPMYEHYQKNHLMCIRLRNPWG-QKEWNGSLSDASEEWHSISHKDKIAMGWKVENDGEFWMLIDDVLVNFNEITI--IRPIKTGIFKYNSN---WFGSMCKGYWT-GV-AGDRQSALAEIGQGGQFKIEINNPKGD------IFLCHLLQKCARGSEA------ALGILIIAVERNRDFMVHTPKFCN-VKLEYRLGGS------RSICLRYRLTCGTYIVKPFLTVASPSLKFPIYYLLRCYTTQKNN 1510
Q+Y ++ + +LF D F A ++S+ S + + IEW RP E+ NP F+ G S DV QG LG+CWL++A A L Q +VIP +Q + N Y G+F W+YG+W++V+IDDRLP+ NG+L+++HS KNEFWSAL+EKAYAK+ GSYE+L GG TCE+M DFTGG+ E +DLK+ NLF +L + S+M +I + E + GLI HAYSIT VC + I TP ++ + IR+RNPWG + EWNG SD+S EW I + K +G + DGEFWM D L +F+ + I + P + NS W SM +G WT GV AG ++ L Q+ I + +P + + L+QK R +G I ++ N + + P+ N + + +G S R +C R++L G Y++ P + +F ++R ++ +NN
Sbjct: 73 QDYETILNSCLASGSLFEDPLFPASNESLQFSRRPD--RHIEWLRPHEIAENPQFFVEGYSRFDVQQGELGDCWLLAATANLTQESNLFFRVIPA--EQSFEEN----YAGIFHFRFWQYGKWVDVIIDDRLPTYNGELMYMHSTEKNEFWSALLEKAYAKLHGSYEALKGGSTCEAMEDFTGGVSE-WYDLKEAPG------NLFTILQKAAERNSMMGCSIEPDPNVTEAETPQGLIRGHAYSITKVCLIDI-VTP----NRQGKIPMIRMRNPWGNEAEWNGPWSDSSPEWRYIPEEQKAEIGLTFDRDGEFWMSFQDFLNHFDRVEICNLSPDSLTEDQQNSGKRKWEMSMYEGEWTPGVTAGGCRNFLDTFWHNPQYIITLVDPDEEDEEGQCTVIVALMQKNRRSKRNMGMECLTIGFAIYSL--NDRELENRPQGLNFFRYKSSVGRSPHFINTREVCARFKLPPGHYLIVPSTFDPNEEGEF----IIRVFSETQNN 551
BLAST of Calpain 5a vs. TrEMBL
Match: A0A3Q2QZ28 (Calpain 5a OS=Fundulus heteroclitus OX=8078 PE=3 SV=1) HSP 1 Score: 384.03 bits (985), Expect = 1.035e-119 Identity = 224/610 (36.72%), Postives = 345/610 (56.56%), Query Frame = 2
Query: 68 FNNQNYVSMRQEHQKNLTLFCDDTFGADDKSISPSGKQEILSSIEWKRPSELCINPCLFIAGSSVHDVCQGSLGNCWLVSALAVLAQHPECLKKVIPNVDDQEWHGNRPHSYTGMFRICLWRYGEWLEVVIDDRLPSRNGKLVFVHSKVKNEFWSALIEKAYAKVCGSYESLVGGLTCESMVDFTGGIPEQIFDLKKYSKSDTEMEN-LF-KLLHFDWKDGSLMCATIASRSDEMEEKLANGLITTHAYSITGVCKLRIKSTPMYEHYQKNHLMCIRLRNPWGQKEWNGSLSDASEEWHSISHKDKIAMGWKVENDGEFWMLIDDVLVNFNEITIIRPIKTGIFKYNSNWFGSMCKGYWT-----------GVAGDRQSALAEIGQGGQFKIEINNPKGDIFLC------HLLQKCARGSEAALGILIIAVERNRDFMVHTPKFCNVKLEYRLGG-----SRSICLRYRLTCGTYIVKPFLTVASPSLKFPIYYLLRCYTTQKNNGTFLPSEMPILERNC---ICKSNCTIIICRLHXXXXXXXXXXEELDVLNIFCEIDCENQSLRTTVNCNTSGLFWDETFLLHRSKMDRPIIIKIFHRGRFFNTLIG 1816
+ Q++V++R++ ++N LF D F A D+S+ + + + WKRP EL +P LF+ G S HD+ QG LGNCW V+A + LA +KVIP+ +QEW P SY G+F WR+G W++VVIDDRLP+ NGKLV+ HS KNEFWSAL+EKAYAK+CG YE+L GG T +++VDFTGG+ E + + K D E N LF ++L +DG + C+ A +++ME KLA GL+ HAY++T V ++R+ + +++ + L IR+RNPWGQ+EWNG SD+SEEW +S ++ MG V++DGEFWM DD + NF ++ + R I T + W ++ +G W G + ++QS L Q Q+K ++ PK ++ +C + RG +G I VE NR + +H ++ ++GG SRS+ LR LT G Y++ P T +P L+ +LLR + + T L + P + C IC ++ ++H++QAD + + V + + I CE Q +R+ V NT ++ L +R K +RPI I+I++ F ++ +G
Sbjct: 5 YEEQSFVALRRQCRQNGNLFEDLLFPASDQSLFYQSNR--IGHVTWKRPKELSPDPHLFVDGISAHDLHQGQLGNCWFVAACSSLASREALWQKVIPDWKEQEWDKENPDSYAGIFHFQFWRFGRWVDVVIDDRLPTVNGKLVYCHSNDKNEFWSALVEKAYAKMCGCYEALDGGNTADALVDFTGGVSEPVDLTENGYKDDEEKRNDLFERVLKVHNRDGLISCSIRAITAEDMEAKLACGLVKGHAYAVTDVRRVRLGHG-LLSYFKSDKLNMIRMRNPWGQREWNGPWSDSSEEWKKVSKSERERMGVTVQDDGEFWMTFDDFIANFTDLILCRLINTSYLSLHKTWEEAVMRGSWRRHDDPLCNRAGGCSNNKQSFL----QNPQYKFDVKKPKDEVLICLQQEDRRATLREGRGENLPIGFDIQRVELNRTYRMHA-------VQEKVGGSIYINSRSVFLRTELTEGRYVIIP--TTFNPGLEGE--FLLRIFADVPTDCTELTLDEP--PQTCWSGIC--GYPSLVTQVHVLQADGLAGQDSDGVSDPYVIIRCEGQKVRSAVYQNTRSPAFNTKGLFYRKKANRPISIEIYNHNVFKDSFLG 592
BLAST of Calpain 5a vs. TrEMBL
Match: A0A3Q3EVJ7 (Calpain 5a OS=Labrus bergylta OX=56723 PE=3 SV=1) HSP 1 Score: 381.719 bits (979), Expect = 7.906e-119 Identity = 220/613 (35.89%), Postives = 346/613 (56.44%), Query Frame = 2
Query: 62 VFFNNQNYVSMRQEHQKNLTLFCDDTFGADDKSISPSGKQEILSSIEWKRPSELCINPCLFIAGSSVHDVCQGSLGNCWLVSALAVLAQHPECLKKVIPNVDDQEWHGNRPHSYTGMFRICLWRYGEWLEVVIDDRLPSRNGKLVFVHSKVKNEFWSALIEKAYAKVCGSYESLVGGLTCESMVDFTGGIPEQIFDLKKYSKSDTE-MENLF-KLLHFDWKDGSLMCATIASRSDEMEEKLANGLITTHAYSITGVCKLRIKSTPMYEHYQKNHLMCIRLRNPWGQKEWNGSLSDASEEWHSISHKDKIAMGWKVENDGEFWMLIDDVLVNFNEITIIRPIKTGIFKYNSNWFGSMCKGYWT-----------GVAGDRQSALAEIGQGGQFKIEINNPKGDIFLCHLLQKC-------ARGSEAALGILIIAVERNRDFMVHTPKFCNVKLEYRLGG-----SRSICLRYRLTCGTYIVKPFLTVASPSLKFPIYYLLRCYTTQKNNGTFLPSEMPILERNC---ICKSNCTIIICRLHXXXXXXXXXXEELDVLNIFCEIDCENQSLRTTVNCNTSGLFWDETFLLHRSKMDRPIIIKIFHRGRFFNTLIG 1816
V + Q++ ++R++ Q+ LF D F D+S+ + + S+ WKRP ELC NP LF+ G S HD+ QG LGNCW V+A + LA +KVIP+ DQEW ++P SY G+F WR+GEW++VVIDDRLP+ NG+LV+ HS NEFWSAL+EKAYAK+CG YE+L GG T +++VDFTGG+ E + ++ K D E + LF ++L ++G + C+ A+ + +ME +LA GL+ HAY++T V ++R+ + +++ + L IR+RNPWGQ+EWNG SD+SEEW +S ++ +G V++DGEFWM DD + NF ++ + R I T ++ W ++ +G W G ++QS L Q Q+ ++ P+ +I +C L QK RG A+G I VE NR + +H + ++GG SRS+ +R L G Y++ P T P L+ +LLR +T +N L + P + C +C ++ ++HI++A + + + + I CE Q +R+ VN +T +D L +R K+ +PI IK+++ ++ +G
Sbjct: 3 VSYVGQSFSALRKQCQQKGVLFEDPLFPTTDQSLFYQSNR--IGSVTWKRPKELCSNPHLFVDGISAHDLHQGQLGNCWFVAACSSLASREALWRKVIPDWKDQEWDEDKPESYAGIFHFRFWRFGEWVDVVIDDRLPTANGELVYCHSNDSNEFWSALVEKAYAKMCGCYEALDGGNTADALVDFTGGVSEPLDLVETGFKDDKEKRDELFERVLKVHNREGLISCSIRATTAADMEARLACGLVKGHAYAVTDVRRVRL-GHGLLAYFRSDKLTMIRMRNPWGQREWNGPWSDSSEEWKKVSSSEREKIGVTVQDDGEFWMTFDDFIANFTDLILCRLINTSYLSFHKTWEETVIRGSWIRNGDTLLNRTGGCTNNKQSFL----QNPQYVFDVKKPEDEILIC-LQQKDRRASLREGRGENLAIGFDIHRVELNRTYRMHA-------TQEKVGGSTYINSRSVSVRLELKEGRYVIIP--TTFDPGLEGE--FLLRIFTDVPSNCKELTLDKP--PKTCWSGLC--GYPSLVTQVHILRAAGLAGQDSDGASDPYVIISCEGQKVRSPVNKDTRSPAFDTKGLFYRKKVKQPISIKLYNHNLLIDSFLG 592
BLAST of Calpain 5a vs. TrEMBL
Match: A0A4Z2IKQ2 (Calpain-5 OS=Liparis tanakae OX=230148 GN=CAPN5_0 PE=3 SV=1) HSP 1 Score: 379.407 bits (973), Expect = 8.126e-118 Identity = 221/615 (35.93%), Postives = 345/615 (56.10%), Query Frame = 2
Query: 53 RNFVFFNNQNYVSMRQEHQKNLTLFCDDTFGADDKSISPSGKQEILSSIEWKRPSELCINPCLFIAGSSVHDVCQGSLGNCWLVSALAVLAQHPECLKKVIPNVDDQEWHGNRPHSYTGMFRICLWRYGEWLEVVIDDRLPSRNGKLVFVHSKVKNEFWSALIEKAYAKVCGSYESLVGGLTCESMVDFTGGIPEQIFDLKKYSKSDTEMEN-LF-KLLHFDWKDGSLMCATIASRSDEMEEKLANGLITTHAYSITGVCKLRIKSTPMYEHYQKNHLMCIRLRNPWGQKEWNGSLSDASEEWHSISHKDKIAMGWKVENDGEFWMLIDDVLVNFNEITIIRPIKTGIFKYNSNWFGSMCKGYWT-------GVAGDRQSALAEIGQGGQFKIEINNPKGDIFLCHLLQKC-------ARGSEAALGILIIAVERNRDFMVHTPKFCNVKLEYRLGG-----SRSICLRYRLTCGTYIVKPFLTVASPSLKFPIYYLLRCYTTQKNNGTFLPS---EMPILERNCICKSNC---TIIICRLHXXXXXXXXXXEELDVLNIFCEIDCENQSLRTTVNCNTSGLFWDETFLLHRSKMDRPIIIKIFHRGRFFNTLIG 1816
R V + Q++ ++R++ Q+N +LF D F D+S+ + + S+ WKRP ELC NP LF+ G S HD+ QG LGNCW V+A + LA +KVIP+ DQEW +P SY G+F WR+GEW +VVIDDRLP+ NG+LV+ HS NEFWSAL+EKAYAK+CG YE+L GG T +++VDFTGG+ E + ++ K D E N LF ++L + G + C+ A+ + +ME +L+ GL+ HAY++T V ++R+ + +++ + L IR+RNPWG+KEWNG+ SD+SEEW +S ++ +G V +DGEFWM DD +VNF ++ + R I T ++ W S+ +G W AG + Q Q+ ++ P+ ++ +C L QK RG A+G I VE NR + +H + ++GG SRS+ +R L G Y++ P T P L+ +LLR +T +PS E+ + E C S ++ ++H++ A+ + + V + + I CE + +R+ V+ NT +D L +R K ++PI I+I++ ++ +G
Sbjct: 6 RMVVPYEGQSFSALRRQCQQNGSLFEDPLFPTSDQSLFYQSNR--IGSVTWKRPKELCDNPHLFVDGISAHDLHQGQLGNCWFVAACSSLASREALWQKVIPDWKDQEWDEEKPESYAGIFHFRFWRFGEWADVVIDDRLPTANGELVYCHSNDSNEFWSALVEKAYAKMCGCYEALDGGNTADALVDFTGGVSEPMDLMENGFKDDDEKRNELFERVLKVHDRGGLISCSIRATTAADMEARLSCGLVKGHAYAVTDVRRVRLGHG-LLAYFRSDKLTMIRMRNPWGEKEWNGAWSDSSEEWKKVSKSERERIGVTVRDDGEFWMTFDDFIVNFTDLILCRLINTSYLSFHKTWEESVMRGSWQRHDEPLLNRAGGCTNQKHSFLQNPQYVFDVKKPEDEVLIC-LQQKDRRATLREGRGENLAIGFEIHRVEFNRTYRMHV-------TQQKVGGSVYINSRSVFIRIELPEGRYVIIP--TTFDPDLEG--VFLLRTFTD-------VPSDCKELTLHEPPQTCWSGLCGYPSLVTQIHVLNANGLAGQDSDGVSDPYVLIRCEGEKVRSPVHKNTRSPNFDTKGLFYRKKANQPIRIEIYNHNVLMDSFLG 598
BLAST of Calpain 5a vs. TrEMBL
Match: A0A4W6G005 (Uncharacterized protein OS=Lates calcarifer OX=8187 GN=LOC108872711 PE=3 SV=1) HSP 1 Score: 375.17 bits (962), Expect = 2.801e-116 Identity = 219/613 (35.73%), Postives = 346/613 (56.44%), Query Frame = 2
Query: 62 VFFNNQNYVSMRQEHQKNLTLFCDDTFGADDKSISPSGKQEILSSIEWKRPSELCINPCLFIAGSSVHDVCQGSLGNCWLVSALAVLAQHPECLKKVIPNVDDQEWHGNRPHSYTGMFRICLWRYGEWLEVVIDDRLPSRNGKLVFVHSKVKNEFWSALIEKAYAKVCGSYESLVGGLTCESMVDFTGGIPEQIFDLKKYSKSDTEMEN-LF-KLLHFDWKDGSLMCATIASRSDEMEEKLANGLITTHAYSITGVCKLRIKSTPMYEHYQKNHLMCIRLRNPWGQKEWNGSLSDASEEWHSISHKDKIAMGWKVENDGEFWMLIDDVLVNFNEITIIRPIKTGIFKYNSNWFGSMCKGYWT-----------GVAGDRQSALAEIGQGGQFKIEINNPKGDIFLCHLLQKC-------ARGSEAALGILIIAVERNRDFMVHTPKFCNVKLEYRLGG-----SRSICLRYRLTCGTYIVKPFLTVASPSLKFPIYYLLRCYTTQKNNGTFLPSEMPILERNCICKSNC---TIIICRLHXXXXXXXXXXEELDVLNIFCEIDCENQSLRTTVNCNTSGLFWDETFLLHRSKMDRPIIIKIFHRGRFFNTLIG 1816
V + Q++ ++R++ Q+N LF D F A D+S+ + + WKRP ELC +P LF+ G S HD+ QG LGNCW V+A + LA +KVIP+ DQEW+ +P SY G+F WR+GEW++VVIDD+LP+ NG+LV+ HSK NEFWSAL+EKAYAK+CG YE+L GG T +++VDFTGG+ E + + K D E N LF ++L + G + C+ A+ +++ME +LA GL+ HAY++T V K+R+ + +++ + L IR+RNPWGQ+EWNG SD+SEEW +S ++ +G V++DGEFWM DD + NF ++ + R I T + W ++ +G W G ++++ L Q Q+ ++ P+ +I +C L QK RG A+G I VE NR + +H + ++GG SRS+ LR L G Y++ P T P L+ +LLR ++ ++ E+ + E C S ++ ++HI+QA+ + + V + + I CE + +R+ ++ NT +D L +R K ++PI I+I++ ++ +G
Sbjct: 3 VPYEGQSFSALRRQCQQNGRLFVDPLFPASDQSLFYQSNH--IGRVTWKRPKELCSDPHLFVDGISAHDLHQGQLGNCWFVAACSSLASREALWQKVIPDWKDQEWNDEKPESYAGIFHFRFWRFGEWVDVVIDDQLPTVNGELVYCHSKDSNEFWSALVEKAYAKMCGCYEALDGGNTADALVDFTGGVSEPMNLTENGFKEDEEKRNELFERVLKVHNRGGLISCSIRATSAEDMEARLACGLVKGHAYAVTDVRKVRLGHG-LLAYFRSDKLTMIRMRNPWGQREWNGPWSDSSEEWKKVSKSEREKIGVTVQDDGEFWMTFDDFIANFTDLILCRLINTSYLSLHKTWEEAVMRGSWRRHDDPLLNRAGGCTNNKRTFL----QNPQYVFDVKKPEDEILIC-LQQKDRRARLKEGRGENLAIGFDIHRVELNRIYRMHV-------TQQKVGGSVYINSRSVFLRIDLKEGRYVIIP--TTFDPGLEGE--FLLRIFSDVSSDC----KELTVDEPPQTCWSGLCGYPSLVTQVHILQANGLAGQDSDGVSDPYVIIRCEGEKVRSPIHKNTRSPAFDTKGLFYRKKANQPISIEIYNHNVLMDSFLG 592
BLAST of Calpain 5a vs. TrEMBL
Match: R7VLA7 (Uncharacterized protein OS=Capitella teleta OX=283909 GN=CAPTEDRAFT_228346 PE=3 SV=1) HSP 1 Score: 371.703 bits (953), Expect = 5.275e-115 Identity = 214/603 (35.49%), Postives = 334/603 (55.39%), Query Frame = 2
Query: 53 RNFVFFNNQNYVSMRQEHQKNLTLFCDDTFGADDKSISPSGKQEILSSIEWKRPSELCINPCLFIAGSSVHDVCQGSLGNCWLVSALAVLAQHPECLKKVIPNVDDQEWHGNRPHSYTGMFRICLWRYGEWLEVVIDDRLPSRNGKLVFVHSKVKNEFWSALIEKAYAKVCGSYESLVGGLTCESMVDFTGGI--PEQIFDLKKYSKSDTEMENLFKLLHFDWKDGSLMCATIASRS-DEMEEKLANGLITTHAYSITGVCKLRIKSTPMYEHYQKNHLMCIRLRNPWGQKEWNGSLSDASEEWHSISHKDKIAMGWKVENDGEFWMLIDDVLVNFNEITIIRPIKTGIFKYNSNWFGSMCKGYWT------GVAGDRQSALAEIGQGGQFKIEINNPKGDIFLCHLLQKCARGSEA----ALGILIIAVERNRDFMVHTPKFCNVKLEYRLGGSRSICLRYRLTCGTYIVKPFLTVASPSLKFPIYYLLRCYTTQKNNGTFLPSEMPILER-NCICKSNCTIIICRLHXXXXXXXXXXEELDVLNIFCEIDCENQSLRTTVNCNTSGLFWDETFLLH-RSKMDRPIIIKIFHRGRFFNTLIG 1816
++ V F +Q+Y +R+E Q + LF D TF A++ SI + + IEWKRP ++ NP LF+ G + D+ QG LGNCW V+A + LA + E +KVIPN DQEW +P Y G++ WR+GEW +VVIDD LP+RNG LV+ HSK +NEFWSAL+EKAYAK+ GSYESL GG E++ DFTGG+ P + DL K + +L+K++ + ++M A I S + +E EE + GL+ HAY IT V ++ ++ T ++ + K + IRLRNPWG EW G+ SD SEEW I + + +G ++DGEFWM +D +F ++I R I T +F + + W + G W G ++++ L + Q+ ++++ D L L QKC RG E +G I+ VE NR + VH + + SR I LR+ L G Y++ P++K +LLR YTT ++ L + P + +C+ ++ R+ II+A ++ + + + +C+I CE +T+ +T W+ +++ + R+ +PI I+I++ NT +G
Sbjct: 4 KSVVLFKDQDYDKLRKEAQSSGQLFTDPTFPANEASIGGNA-----AGIEWKRPKDIAENPSLFVDGVTCSDLNQGELGNCWFVAACSCLATNKEIWRKVIPNHKDQEWDPEKPEKYAGIYYFRFWRFGEWTDVVIDDLLPTRNGDLVYAHSKDRNEFWSALLEKAYAKLYGSYESLDGGELAEALEDFTGGVSEPLNLVDL-KVGIEEEARNDLYKVMRKAMDNHAMMAAAIPSATKEEQEEVMDMGLVKGHAYGITAVKRIALEGTGIFNMFNKEKIAMIRLRNPWGGTEWKGAFSDESEEWKKIDKESREKIGLTFDDDGEFWMNFEDYCKHFTNMSICRVINTSLFSFQTTWTEACMHGEWVTPHFSGGCVNNKETFL----KNPQYVFDVDD-NDDECLFQLTQKCGRGGEGIEKTTIGFSIMKVEYNRKYRVH--QMQRITKSSAFKNSRGIFLRHTLDKGRYVI--VACTFEPNVK--AKHLLRVYTTGTSHAHALVKDKPSEKTLSCLPWFAYPSVVTRVTIIRARDLEKQDTIGGADPYCQISCEGHHYKTSTIKDTVNPEWNSSYIFYRRNHRSKPIEIEIWNSNALKNTFMG 589
BLAST of Calpain 5a vs. Ensembl Cavefish
Match: capn5a (calpain 5 [Source:NCBI gene;Acc:103038917]) HSP 1 Score: 334.724 bits (857), Expect = 3.064e-104 Identity = 202/615 (32.85%), Postives = 333/615 (54.15%), Query Frame = 2
Query: 68 FNNQNYVSMRQEHQKNLTLFCDDTFGADDKSISPSGKQEILSSIEWKRPSELCINPCLFIAGSSVHDVCQGSLGNCWLVSALAVLAQHPECLKKVIPNVDDQEWHGNRPHSYTGMFRICLWRYGEWLEVVIDDRLPSRNGKLVFVHSKVKNEFWSALIEKAYAKVCGSYESLVGGLTCESMVDFTGGIPEQIFDLK-KYSKSDTEMENLF-KLLHFDWKDGSLMCATIASRSDEMEEKLANGLITTHAYSITGVCKLRIKSTPMYEHYQKNHLMCIRLRNPWGQKEWNGSLSDASEEWHSISHKDKIAMGWKVENDGEFWMLIDDVLVNFNEITIIRPIKTGIFKYNSNWFGSMCKGYWT-----------GVAGDRQSALAEIGQGGQFKIEINNPKGDIFLCHLLQKCAR-------GSEAALGILIIAVERNRDFMVHTP--KFCNVKLEYRLGGSRSICLRYRLTCGTYIVKPFLTVASPSLKFPIYYLLRCYTTQKNNGTFLPSEMPILERNC---ICKSNCTIIICRLHXXXXXXXXXXEELDVLNIFCEIDCENQSLRTTVNCNTSGLFWDETFLLHRSKMDRPIIIKIFHRGRFFNTLIGYSCCSYD 1837
+ Q + ++R+ Q++ TLF D F D+S+ Q + + WKRP ELC +P LF+ G S HD+ QG LGNCW V+A + LA +KVIP+ DQEW N P SY G+F W +GEW++VVIDDRLP+ NG L++ HS NEFWSAL+EKAYAK+ G YE+L GG T +++VDFTGG+ E + L+ ++ + + LF ++L + G + C+ A+ +ME +L GL+ HAY++T V ++R+ + +++ LM IR+RNPWG++EWNG SD+SEEW +S ++ +G V++DGEFWM+ +D +F ++ + R I T + W ++ G W+ G ++ + L Q Q+ ++ P+ ++ +C L QK R G A+G + VE NR + +HT K C+ SRS+ +R L G Y++ P T +P L+ +LLR +T ++ L + P + C +C ++ ++H+++ +Q + + + I CE +++ + + +D L +R K + I I+++++ ++ +G S D
Sbjct: 8 YEGQQFEALRKRCQQSRTLFEDPLFPPVDESLFYQTNQ--IGKVTWKRPKELCSDPHLFVNGISAHDLHQGRLGNCWFVAACSSLASREALWQKVIPDWKDQEWDSNHPDSYAGIFHFRFWLFGEWVDVVIDDRLPTANGNLIYCHSNDSNEFWSALVEKAYAKIHGCYEALDGGNTADALVDFTGGVSEPVDLLEGRFGQEEEARNQLFDRVLKVHNRGGLISCSIRATTQADMEARLDCGLVKGHAYAVTDVRRVRLGHG-LLAYFKSEKLMMIRMRNPWGEREWNGPWSDSSEEWQKVSKGEREKLGVTVQDDGEFWMMFEDFSKHFTDLILCRLINTSYLSIHKTWEEAVMMGSWSRHDDPLSNRSGGCINNKTTYL----QNPQYVFDVKKPEDEVLIC-LQQKDRRARPKEGKGENLAIGFDLQRVELNRVYRMHTSQQKLCSSVYI----NSRSVFVRKDLKEGRYVIIP--TTFNPGLQGD--FLLRVFTDVPSDCKELTLDEP--PQTCWTGLC--GYPQLVTQVHVMKGVGLQGQDSDGSSDPYIIISCEGVKVKSPAHKSNLSPEFDVKALFYRKKPGQGIHIEVYNKNVLNDSFMGQVTLSGD 602
BLAST of Calpain 5a vs. Ensembl Cavefish
Match: capn5b (calpain-5-like [Source:NCBI gene;Acc:103035636]) HSP 1 Score: 326.25 bits (835), Expect = 5.371e-101 Identity = 205/607 (33.77%), Postives = 325/607 (53.54%), Query Frame = 2
Query: 68 FNNQNYVSMRQEHQKNLTLFCDDTFGADDKSISPSGKQEILSSIEWKRPSELCINPCLFIAGSSVHDVCQGSLGNCWLVSALAVLAQHPECLKKVIPNVDDQEWHGNRPHSYTGMFRICLWRYGEWLEVVIDDRLPSRNGKLVFVHSKVKNEFWSALIEKAYAKVCGSYESLVGGLTCESMVDFTGGIPEQIFDLKKYSKSDTEMEN-LF-KLLHFDWKDGSLMCATIASRSDEMEEKLANGLITTHAYSITGVCKLRIKSTPMYEHYQKNHLMCIRLRNPWGQKEWNGSLSDASEEWHSISHKDKIAMGWKVENDGEFWMLIDDVLVNFNEITIIRPIKTGIFKYNSNWFGSMCKGYWT-------GVAGDRQSALAEIGQGGQFKIEINNPKGDIFLCHLLQKCAR-------GSEAALGILIIAVERNRDFMVHTPKFCNVKLEYRLGG-----SRSICLRYRLTCGTYIVKPFLTVASPSLKFPIYYLLRCYTTQKNNGTFLPSEMPILERNC---ICKSNCTIIICRLHXXXXXXXXXXEELDVLNIFCEIDCENQSLRTTVNCNTSGLFWDETFLLHRSKMDRPIIIKIFHRGRFFNTLIG 1816
F Q Y +++++ ++ TLF D F A D S+ G + + + WKRP ELC +P LF+ G S HD+ QG LGNCW V+A + LA +KVIP+ +QEW + SY G+F WR+GEW++VVIDDRLP+ + +LV+ HS NEFWSAL+EKAYAKV G YE+L GG T +++VDFTGG+ E + L+ D N LF ++L +DG + C+ A+ ++ME +L GL+ HAY++T V K+R+ + ++ L IR+RNPWG+KEW+G SD+SEEW +S ++ +G V++DGEFWM DD F ++ + R I T + W + +G W +G + A Q Q+ ++ + ++ +C L QK R G A+G I VE NR + +H+ + ++ G SR + LR L G Y++ P T P + +LLR +T ++ L + P + C +C ++ ++H++ A+ +Q + + + I CE + +R+ V+ +T +D + R K I I+I+++ +T +G
Sbjct: 8 FEGQQYSTLKKQCLQSGTLFEDPLFPAVDDSLFYQGNR--IGRVHWKRPRELCDDPHLFVDGISAHDLHQGQLGNCWFVAACSSLASRESLWQKVIPDWREQEWDPEKDESYVGIFHFRFWRFGEWVDVVIDDRLPTVDNQLVYCHSNDSNEFWSALVEKAYAKVYGCYEALDGGNTADALVDFTGGVSEPMDLLEGQMAQDEVARNQLFERVLKVHNRDGLISCSIRATTVEDMEARLDCGLVKGHAYAVTDVKKVRL-GHGLLAFFKSEKLHMIRMRNPWGEKEWSGPWSDSSEEWKKVSKSEREKLGVTVQDDGEFWMNFDDFCQYFTDLILCRLINTSYLSIHKTWEEEVMRGTWVYRDDPLRNRSGGCINHKATFLQNPQYVFDVRKVEDEVLIC-LQQKEKRATPKEGKGENLAIGFDIHRVELNRKYRMHSA-------QQKVAGSIYINSRCVFLRKELKEGRYVIIP--TTFDPGQQGE--FLLRVFTDVPSDCKELTLDDP--PQTCWTGMC--GYPQLVTQVHVLSAEGLQGQDSNGASDPYVIITCEGEKVRSPVHKDTRCPNFDVKGVFFRKKPKEGIHIEIYNKNVIVDTFLG 595
BLAST of Calpain 5a vs. Ensembl Cavefish
Match: capn5b (calpain-5-like [Source:NCBI gene;Acc:103035636]) HSP 1 Score: 317.775 bits (813), Expect = 5.608e-98 Identity = 202/594 (34.01%), Postives = 317/594 (53.37%), Query Frame = 2
Query: 68 FNNQNYVSMRQEHQKNLTLFCDDTFGADDKSISPSGKQEILSSIEWKRPSELCINPCLFIAGSSVHDVCQGSLGNCWLVSALAVLAQHPECLKKVIPNVDDQEWHGNRPHSYTGMFRICLWRYGEWLEVVIDDRLPSRNGKLVFVHSKVKNEFWSALIEKAYAKVCGSYESLVGGLTCESMVDFTGGIPEQIFDLKKYSKSDTEMEN-LF-KLLHFDWKDGSLMCATIASRSDEMEEKLANGLITTHAYSITGVCKLRIKSTPMYEHYQKNHLMCIRLRNPWGQKEWNGSLSDASEEWHSISHKDKIAMGWKVENDGEFWMLIDDVLVNFNEITIIRPIKTGIFKYNSNWFGSMCKGYWT-------GVAGDRQSALAEIGQGGQFKIEINNPKGDIFLCHLLQKCAR-------GSEAALGILIIAVERNRDFMVHTPKFCNVKLEYRLGG-----SRSICLRYRLTCGTYIVKPFLTVASPSLKFPIYYLLRCYTTQKNNGTFLPSEMPILERNC---ICKSNCTIIICRLHXXXXXXXXXXEELDVLNIFCEIDCENQSLRTTVNCNTSGLFWDETFLLHRSKMDRPIIIK 1777
F Q Y +++++ ++ TLF D F A D S+ G + + + WKRP ELC +P LF+ G S HD+ QG LGNCW V+A + LA +KVIP+ +QEW + SY G+F WR+GEW++VVIDDRLP+ + +LV+ HS NEFWSAL+EKAYAKV G YE+L GG T +++VDFTGG+ E + L+ D N LF ++L +DG + C+ A+ ++ME +L GL+ HAY++T V K+R+ + ++ L IR+RNPWG+KEW+G SD+SEEW +S ++ +G V++DGEFWM DD F ++ + R I T + W + +G W +G + A Q Q+ ++ + ++ +C L QK R G A+G I VE NR + +H+ + ++ G SR + LR L G Y++ P T P + +LLR +T ++ L + P + C +C ++ ++H++ A+ +Q + + + I CE + +R+ V+ +T +D + R K I I+
Sbjct: 8 FEGQQYSTLKKQCLQSGTLFEDPLFPAVDDSLFYQGNR--IGRVHWKRPRELCDDPHLFVDGISAHDLHQGQLGNCWFVAACSSLASRESLWQKVIPDWREQEWDPEKDESYVGIFHFRFWRFGEWVDVVIDDRLPTVDNQLVYCHSNDSNEFWSALVEKAYAKVYGCYEALDGGNTADALVDFTGGVSEPMDLLEGQMAQDEVARNQLFERVLKVHNRDGLISCSIRATTVEDMEARLDCGLVKGHAYAVTDVKKVRL-GHGLLAFFKSEKLHMIRMRNPWGEKEWSGPWSDSSEEWKKVSKSEREKLGVTVQDDGEFWMNFDDFCQYFTDLILCRLINTSYLSIHKTWEEEVMRGTWVYRDDPLRNRSGGCINHKATFLQNPQYVFDVRKVEDEVLIC-LQQKEKRATPKEGKGENLAIGFDIHRVELNRKYRMHSA-------QQKVAGSIYINSRCVFLRKELKEGRYVIIP--TTFDPGQQGE--FLLRVFTDVPSDCKELTLDDP--PQTCWTGMC--GYPQLVTQVHVLSAEGLQGQDSNGASDPYVIITCEGEKVRSPVHKDTRCPNFDVKGVFFRKKPKEGIHIE 582
BLAST of Calpain 5a vs. Ensembl Cavefish
Match: ENSAMXT00000048279.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02000063.1:113453:132090:1 gene:ENSAMXG00000038709.1 transcript:ENSAMXT00000048279.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 285.804 bits (730), Expect = 5.573e-86 Identity = 192/604 (31.79%), Postives = 300/604 (49.67%), Query Frame = 2
Query: 74 NQNYVSMRQEHQKNLTLFCDDTFGADDKSI----SPSGKQEILSSIEWKRPSELCINPCLFIAGSSVHDVCQGSLGNCWLVSALAVLAQHPECLKKVIPNVDDQEWHGNRPHSYTGMFRICLWRYGEWLEVVIDDRLPSRNGKLVFVHSKVKNEFWSALIEKAYAKVCGSYESLVGGLTCESMVDFTGGIPEQI-FDLKKYSKSDTEMENLFKLLHFDWKDGSLMCATIASRSDEMEEKLANGLITTHAYSITGVCKLRIKSTPMYEHYQKNHLMCIRLRNPWGQKEWNGSLSDASEEWHSISHKDKIAMGWKVENDGEFWMLIDDVLVNFNEITIIRPIKTGIFKYNSNWFGSMCKGYWT-----------GVAGDRQSALAEIGQGGQFKIEINNPKGDIFLCHLLQK-------CARGSEAALGILIIAVERNRDFMVHTPKFCNVKLEYRLGGSRSICLRYRLTCGTYIVKPFLTVASPSLKFPIYYLLRCYTTQKNNGTFLPSEMPILERNCICKSNCTIIICRLHXXXXXXXXXXEELDVLNIFCEIDCENQSLRTTVNCNTSGLFWDETFLLHRSKMDRPIIIKIFHRGRFFNTLIG 1816
NQ+Y +++E + LF D F ++S+ P G ++WKRP E+C P LF+ G S HD+ QG +GNCW V+A + LA P+ +KVIPN +QEW +RP YTG+F W +GEW +VV+DDRLP+ NGKL++ HSK NEFWSAL+EKAYAK+ G YESL GG T +++VDF+G + E + + + E LF L G ++ +IAS E E +LANGL+ HAYS+TGV ++R+ + +++ + + IR+RNPWG+ EW G+ SD E+ S D + K M DD NF ++ + R + T + W + G W G RQ+ L Q Q+ +++ + D L L Q+ +G +G I VE NR + +H +RS+ LR L G YI+ P + +P + ++LR Y Q + E+ + + + C S+ + + + E D + + I CE S+++ V +T +D + +R K ++PI I++++ + +G
Sbjct: 8 NQHYSELKRECLQQKKLFEDPEFLCSNRSLFYRTPPPG------PVQWKRPGEMCEAPRLFVEGISAHDLNQGEVGNCWFVAACSCLALKPQLWQKVIPNWKEQEWDPDRPDRYTGIFHFQFWIFGEWTDVVVDDRLPTINGKLIYCHSKSNNEFWSALLEKAYAKLSGCYESLNGGNTGDAVVDFSGAVNEPVSLETGSFRTDSKTREKLFSDLLNVNDQGGIISCSIAS-PHERELRLANGLVKGHAYSVTGVRRVRLGHG-LLAYFKNDTVDLIRMRNPWGKHEWRGAWSDRWGEFLS----DDFKLLKK--------MSFDDWCQNFTDVDVCRLMNTSVLSLQKRWREVVHFGSWIKHSEPLRNRSGGCMNHRQTFL----QNPQYILDLQDE--DELLVSLQQRDMKIHRAHGQGENLTIGFAIFKVELNRKYRMHDIITQQNVATSSYINARSVFLRKLLPKGRYIIIP--STFTPGILGD--FMLRVYANQ----DLICRELDVDKPSVRCWSSFMGYPQVVTQVYVHGAEGLENQDSADPYVIISCEKNSIQSEVQKDTRDPQFDIRAIFYRKKPNKPITIQVWNSNVVKDQFMG 577
BLAST of Calpain 5a vs. Ensembl Cavefish
Match: ENSAMXT00000050823.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02000063.1:117226:132090:1 gene:ENSAMXG00000038709.1 transcript:ENSAMXT00000050823.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 276.559 bits (706), Expect = 2.019e-82 Identity = 186/609 (30.54%), Postives = 303/609 (49.75%), Query Frame = 2
Query: 68 FNNQNYVSMRQEHQKNLTLFCDDTFGADDKSI----SPSGKQEILSSIEWKRPSELCINPCLFIAGSSVHDVCQGSLGNCWLVSALAVLAQHPECLKKVIPNVDDQEWHGNRPHSYTGMFRICLWRYGEWLEVVIDDRLPSRNGKLVFVHSKVKNEFWSALIEKAYAKVCGSYESLVGGLTCESMVDFTGGIPEQI-FDLKKYSKSDTEMENLFKLLHFDWKDGSLMCATIASRSDEMEEKLANGLITTHAYSITGVCKLRIKSTPMYEHYQKNHLMCIRLRNPWGQKEWNGSLSDASEEWHSISHKDKIAMGWKVENDGEFWMLIDDVLVNFNEITIIRPIKTGIFKYNSNWFGSMCKGYWT-----------GVAGDRQSALAEIGQGGQFKIEINNPKGDIFLCHLLQK-------CARGSEAALGILIIAVERNRDFMVHTPKFCNVKLEYRLGGSRSICLRYRLTCGTYIVKPFLTVASPSLKFPIYYLLRCYTTQKNNGTFLPSEMPILERNCICKSNCT---IIICRLHXXXXXXXXXXEELDVLNIFCEIDCENQSLRTTVNCNTSGLFWDETFLLHRSKMDRPIIIKIFHRGRFFNTLIG 1816
+ NQ+Y +++E + LF D F ++S+ P G ++WKRP +C P LF+ G S HD+ QG +GNCW V+A + LA P+ +KVIPN +QEW +RP YTG+F W +GEW +VV+DDRLP+ NGKL++ HSK NEFWSAL+EKAYAK+ G YESL GG T +++VDF+G + E + + + E LF L G ++ +IA+ E E +LANGL+ HAYS+TGV ++R+ + +++ + + IR+RNPWG+ EW G+ SD W ++ M DD NF ++ + R + T + W + G W G RQ+ L Q Q+ +++ + D L L Q+ +G +G I VE NR + +H +RS+ LR L G YI+ P + +P + ++LR Y Q + E+ + + + C S+ ++ ++++ A+ ++ + + + I CE S+++ V +T +D + +R K ++PI I++++ + +G
Sbjct: 8 YKNQHYSELKRECLQQKKLFEDPEFLCSNRSLFYRTPPPG------PVQWKRPG-MCEAPRLFVEGISAHDLNQGEVGNCWFVAACSCLALKPQLWQKVIPNWKEQEWDPDRPDRYTGIFHFQFWIFGEWTDVVVDDRLPTINGKLIYCHSKSNNEFWSALLEKAYAKLSGCYESLNGGNTGDAVVDFSGAVNEPVSLETGSFRTDSKTREKLFSDLLNVNDQGGIISCSIAASPHERELRLANGLVKGHAYSVTGVRRVRLGHG-LLAYFKNDTVDLIRMRNPWGKHEWRGAWSD---RWGNVLRTFLCVF---------CRMSFDDWCQNFTDVDVCRLMNTSVLSLQKRWREVVHFGSWIKHSEPLRNRSGGCMNHRQTFL----QNPQYILDLQDE--DELLVSLQQRDMKIHRAHGQGENLTIGFAIFKVELNRKYRMHDIITQQNVATSSYINARSVFLRKLLPKGRYIIIP--STFTPGILGD--FMLRVYANQD----LICRELDVDKPSVRCWSSFMGYPQVVTQVYVHGAEGLENQDSSGGADPYVIISCEKNSIQSEVQKDTRDPQFDIRAIFYRKKPNKPITIQVWNSNVVKDQFMG 582
BLAST of Calpain 5a vs. Ensembl Sea Lamprey
Match: capn9 (calpain 9 [Source:ZFIN;Acc:ZDB-GENE-010724-2]) HSP 1 Score: 239.58 bits (610), Expect = 4.005e-70 Identity = 146/445 (32.81%), Postives = 236/445 (53.03%), Query Frame = 2
Query: 197 IEWKRPSELCINPCLFIAGSSVHDVCQGSLGNCWLVSALAVLAQHPECLKKVIPNVDDQEWHGNRPHSYTGMFRIC--LWRYGEWLEVVIDDRLPSRNGKLVFVHSKVKNEFWSALIEKAYAKVCGSYESLVGGLTCESMVDFTGGIPEQIFDLKKYSKSDTEMENLFKLLHFDWKDGSLM-CATIASRSDEMEEKLANGLITTHAYSITGVCKLRIKSTPMYEHYQKNHLMCIRLRNPWGQKEWNGSLSDASEEWHSISHKDKIAMGWKVENDGEFWMLIDDVLVNFNEITI-----IRPIKTGIFKYNSNWFGSMCKGYWTG--VAGDRQSALAEIGQGGQFKIEINNPKGDI---------FLCHLLQKCARGSEAALGILIIAVER--------NRDFMVH------TPKFCNVKLEYRLGGSRSICLRYRLTCGTYIVKP 1432
++W E+ NP + G++ D+ QG+LG+CWL++A+A L + + L KV+P N Y G+F W +GEW++V IDDRLP++NG+LVFVHS K EFWSAL+EKAY+K+ GSYE+L GG E+M DFTGG+ E + DLK T +LF+++ + GS+M C+ + + E E + +NGL+ HAYSITGV ++ +Y+ + +R+RNPWG+ EWNG+ SD + EW ++ ++ + + DGEFWM DD + +F ++ I R + G +Y W ++ G W AG ++ QF+++++N + F+ L+QK R + + + A+ + ++F ++ +P F N+ R + R+RL G Y++ P
Sbjct: 2 VKWGLSREIVQNPEFIVDGATRTDIKQGALGDCWLLAAIASLTLNDQLLAKVVPPGQ------NFRDGYAGIFHFQQKFWHFGEWMDVFIDDRLPTKNGQLVFVHSADKREFWSALLEKAYSKLNGSYEALKGGSIAEAMEDFTGGVYE-LLDLK------TAPPDLFRIMCKARQKGSMMGCSIDVTNASESEARTSNGLVKGHAYSITGVDEV---------NYRGQKVKLLRIRNPWGEVEWNGAWSDHAPEWKALDQGERNRLQQVMAEDGEFWMSFDDFMRHFTKMEICNLTPDRVEREGDPRY---WTVTVHDGRWLKGCTAGGCRNFPETFWTNPQFRLKLHNEDDEADETGSKACSFIVALMQKNMRKQQRNMLTMGYAIYKIPDQTGPMPKEFFLYNASKARSPSFINL---------REVSQRFRLPAGEYVIVP 412
BLAST of Calpain 5a vs. Ensembl Sea Lamprey
Match: capn3a (calpain 3a, (p94) [Source:ZFIN;Acc:ZDB-GENE-040912-97]) HSP 1 Score: 219.935 bits (559), Expect = 3.178e-61 Identity = 170/536 (31.72%), Postives = 246/536 (45.90%), Query Frame = 2
Query: 119 TLFCDDTFGADDKSISPSGKQEILSSIEWKRPSELCINPCLFIAGSSVHDVCQGSLGNCWLVSALAVLAQHPECLKKVIPNVDDQEWHGNRPHSYTGMFRICLWRYGEWLEVVIDDRLPSRNGKLVFVHSKVKNEFWSALIEKAYAKVCGSYESLVGGLTCESMVDFTGGIPEQIFDLKKYSKSDTEMENLFKLLHFDWKDGSLM-----------CATIASRS------------------------DEMEEKLA----------NGLITTHAYSITGVCKLRIKSTPMYEHYQKNHLMCIRLRNPWGQKEWNGSLSDASEEWHSISHKDKIAMGWKVENDGEFWMLIDDVLVNFNEITIIRPIKTGIFKYNSN-WFGSMCKGYWTG--VAGDRQSALAEIGQGGQFKIEINNPKGDI--------FLCHLLQKCAR------GSEAALGILIIAV-ERNRDFMVHTPK----------FCNVKLEYRLGGSRSICLRYRLTCGTYIVKPFLTVASPSLKFPIYYLLRCYTTQKN 1507
LF D F A + SI S Q +W RP ELC+ + I + H +C+G LG+CW+++A+A + + E L +VIP E +Y G+F WR+GEW++VV+DDRLP+ N +LVFV S +NEFWSAL+EKAYAKV GSYE+L GG T E+M DFTGG+ E F++K K +L+K++ SLM C A S D + K+A GL+ HAYSITG+ +++ +S + +RLRNPWG EW G SDAS+EW+ I+ +K + DGEFW+ D NF ++ I + +N W S+ +G W AG ++ Q+++++ + D F+ L+QK R + +G I V E R H PK CN + R + R+RL G Y++ P A +F LLR + + N
Sbjct: 65 VLFEDPDFPAHNNSIYFS--QNAPMQFQWMRPKELCVPRSIVIEANHTH-ICEGDLGDCWVLAAIACMTMNEELLARVIPAGQSFE------ENYAGIFHFQFWRFGEWVDVVVDDRLPTFNNQLVFVQSAERNEFWSALLEKAYAKVHGSYEALKGGNTTEAMEDFTGGVTE-YFEMKNPPK------DLYKIMVKATSRRSLMGCSIDDGMCWRCGVPAPDSIGLVRSNMSPPILRHIQLTPIGIEDAKKRKIAMLTHAESRTPMGLVRNHAYSITGLDEVKHRS---------KIVQLVRLRNPWGHVEWIGPWSDASKEWNEITPSEKQRLQLISAEDGEFWISFQDFCRNFTKLEICNLSPDALADDRTNKWTVSVSEGRWVRGCSAGGCRNYPDTFWTNPQYRLQLYDEDDDPDDNELLCSFIVALMQKNRRKERRQGSTMLTIGFAIYEVTEELRGSREHLPKDFFLYNASRARCNSYINL-----REVTERFRLPPGEYVIVPSTFEAHQEGEF----LLRVFAEKHN 566
BLAST of Calpain 5a vs. Ensembl Sea Lamprey
Match: capn1a (calpain 1, (mu/I) large subunit a [Source:ZFIN;Acc:ZDB-GENE-040426-1263]) HSP 1 Score: 211.846 bits (538), Expect = 5.038e-59 Identity = 158/531 (29.76%), Postives = 245/531 (46.14%), Query Frame = 2
Query: 53 RNFVFFNNQNYVSMRQEHQKNLTLFCDDTFGADDKSISPSGKQEILSS----IEWKRPSELCINPCLFIAGSSVHDVCQGSLGNCWLVSALAVLAQHPECLKKVIPNVDDQEWHGNRPHSYTGMFRICLWRYGEWLEVVIDDRLPSRNGKLVFVHSKVKNEFWSALIEKAYAKVCGSYESLVGGLTCESMVDFTGGIPEQIFDLKKYSKSDTEMENLFKLLHFDWKDGSLMCATIA--SRSDEMEEKLANGLITTHAYSITGVCKLRIKSTPMYEHYQKNHLMCIRLRNPWGQKEWNGSLSDASEEWHSISHKDKIAMGWKVENDGEFWMLIDDVLVNFNEITIIRPIKTGIFKYNSN-WFGSMCKGYW----TGVAGDRQSALAEIGQGGQFKIEINNPKGDIFLCH-------LLQKCARGSE------AALGILIIAVE-----------RNRDFMVHTPKFCNVKLEYRLGGSRSICLRYRLTCGTYIVKPFLTVASPSLKFPIYYLLRCYTTQKNNGTFLPSEMPI 1540
RNF ++ + +++E + LF D F A S+ + +++ IEWKRP EL +P ++G++ D+ QG+L +CWL+SA+A L +P+ L KV+P Q +H Y G+F +YGEW++V +DDRLP++ G+LVFV S NEFWSAL+EKAYAK+ G YE+L G E++ DFTGG+ E F + + + + + K G+++C I + S E+ GL+ HAYSITGV KL+ + + IR+RNP+G+ E+ G SD S W+ + +D+ + + E DGEFW+ D NF+ + I ++ N W + W R E QF + + + +CH L QK R +GI I R +V +P+F R + +R L G YI+ P A +F LLR + + L S++ +
Sbjct: 21 RNFKT-KSKTFQEIKKECLQRKILFEDPDFPASLSSLHIRVQPTVITQSNNFIEWKRPWELVRHPEFIVSGATRADINQGTLPDCWLLSAIASLTLYPDLLAKVVPM--GQSFH----EDYAGIFLFRFCQYGEWVDVHVDDRLPTKKGELVFVQSGTGNEFWSALLEKAYAKLNGCYEALAYGFPVEALCDFTGGVGE-TFTMNNAPR------DYWDGISRAIKKGAIICCGIETLAASGRGEKVTPMGLVEGHAYSITGVIKLK---------FPGKTVQLIRVRNPYGKVEYTGPWSDGSTTWNEVPEEDQKRLHVRAE-DGEFWISDKDFRKNFHLVEICHQGSEVEIEHRKNLWICELHNSMWLKGLNAGGSRRHRVFKEFPLNYQFFLTLLEEDNVMDVCHGCHFLLSLTQKHRRKFRKIGIDFVPIGIYIYKAVVPGKKLTPQQLRKEKPLVESPEFI---------ARREVTMRMTLPPGNYIIIPCTESAGQEAQF----LLRIFMEKAQQHQGLASQLEV 514
BLAST of Calpain 5a vs. Ensembl Sea Lamprey
Match: ENSPMAT00000000152.1 (pep scaffold:Pmarinus_7.0:GL488881:291:5685:-1 gene:ENSPMAG00000000135.1 transcript:ENSPMAT00000000152.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 184.882 bits (468), Expect = 5.596e-54 Identity = 105/248 (42.34%), Postives = 146/248 (58.87%), Query Frame = 2
Query: 287 GNCWLVSALAVLAQHPECLKKVIPNVDDQEWHGNRPHSYTGMFRICLWRYGEWLEVVIDDRLPSRNGKLVFVHSKVKNEFWSALIEKAYAKVCGSYESLVGGLTCESMVDFTGGIPEQIFDLKKYSKSDTEMENLFKLLHFDWKDGSLM-CATIASRSDEM--EEKLANGLITTHAYSITGVCKLRIKSTPMYEHYQKNHLMCIRLRNPWGQKEWNGSLSDASEEWHSISHKDKIAMGWKVENDGEFW 1021
G+CW ++A++ L + L +V+P Q+ G R H+Y G+F WRYGEW++VV+DDRLP+ GKL FV S NEFW AL+EKAYAKVCGSYE+L G E++VDFTGG+ E Y + +L+ L GSL+ C+ + R E+K +GL+ HAYSITGV ++++ + +RLRNPWG+ EWNG+ SD+S W + K + K+E DGEFW
Sbjct: 1 GDCWFLAAISSLTLNERLLGRVVPM---QQGFGTR-HAYAGIFHFKFWRYGEWVDVVVDDRLPTLKGKLAFVRSCESNEFWGALLEKAYAKVCGSYEALRSGRFTEALVDFTGGVKEH------YLLGEAPPPDLWLFLKRAENAGSLLSCSGVGERVPRTRPEQKTRDGLVLGHAYSITGVERVKVDG---------EVVELVRLRNPWGKVEWNGAWSDSSPVWKCVDCDAKRKLQVKLE-DGEFW 228
BLAST of Calpain 5a vs. Ensembl Sea Lamprey
Match: ENSPMAT00000003998.1 (pep scaffold:Pmarinus_7.0:GL501031:16584:50555:1 gene:ENSPMAG00000003440.1 transcript:ENSPMAT00000003998.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 195.667 bits (496), Expect = 3.396e-53 Identity = 145/505 (28.71%), Postives = 236/505 (46.73%), Query Frame = 2
Query: 206 KRPSELCINPCLFIAGSSVHDVCQGSLGNCWLVSALAVLAQHPECLKKVIPNVDDQEWHGNRPHSYTGMFRICLWRYGEWLEVVIDDRLPSRNGKLVFVHSKVKNEFWSALIEKAYAKVCGSYESLVGGLTCESMVDFTGGIPEQIFDLKKYSKSDTEMENLFKLLHFDWKDGSLM------CATIASRSDEMEEKLANGLITTHAYSITGVCKLR------------------IKSTPMYE-----------------------HYQKNHLMCIRLRNPWGQKEWNGSLSDASEEWHSISHKDKIAMGWKVENDGEFWMLIDDVLVNFNEITI--IRPIKTGIFKYNSNWFGSMCKGYWTG--VAGDRQSALAEIGQGGQFKIEINNPKGDI--------FLCHLLQKCARGSEAALGILIIAVERNRDFMVHTPKFC-NVKLEYRLGGS-----------RSICLRYRLTCGTYIVKPFLTVASPSLKFPIYYLLRCYTTQKN 1507
+ P E+C NP + G++ D+ QG+LG+CWL++A+A L HP L +V+P + Q + Y G+F W++GEW++VVIDD LP++N KL F+HS +NEFWSAL+EKAYAK+ GSYE+L GG E+M DFTGG+ E ++LKK +L++++ + S+M +T RS ++ T A+S +G+ + I+ T M++ ++ + +R+RNPWG+ EWNG+ SD S EW + ++ + DGEFWM D NF+++ I + P + +W + +G W AG ++ QF + ++ D F+ L+QK R + L + A+ + + P C ++ E+ + S R + R+RL G Y++ P + P + +LLR Y + N
Sbjct: 1 RAPQEICYNPQFIVNGATRTDIKQGALGDCWLLAAIASLTLHPRLLARVVP--EGQSF----SDGYAGIFHFQFWQFGEWVDVVIDDLLPTQNNKLAFLHSTDRNEFWSALLEKAYAKLNGSYEALKGGSIAEAMEDFTGGVFE-FYNLKKAPP------DLYRIMRKGLEKASMMGCSIDEGSTFKYRSTPRAINMSKMTATFRAWS-SGIRRFAERFLPSRLGHARLLASSGIEITSMFDLEARLPNGLVKGHAYSITGLDQVFFEGQDVRLVRIRNPWGEVEWNGAWSDNSWEWRVVDVSERRRLEQVRAEDGEFWMSFSDFKRNFSKMEICNLSPDTLRTQSGSRHWTVTAHEGRWLRGCTAGGCRNYAGTFWTNPQFHLRLHEEDDDPDDDEVACSFIVALMQKNMRRQKRDLLTIGYAIY---EIPLKAPPGCTHLPGEFFVRNSSRARSHNFINLREVSDRFRLPPGHYVIVP--STYEPHQEAD--FLLRVYCEKMN 484
BLAST of Calpain 5a vs. Ensembl Nematostella
Match: EDO34860 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SN59]) HSP 1 Score: 357.836 bits (917), Expect = 2.153e-113 Identity = 219/616 (35.55%), Postives = 323/616 (52.44%), Query Frame = 2
Query: 65 FFNNQNYVSMRQEHQKNLTLFCDDTFGADDKSISPSGKQEILSSIEWKRPSELC--INPCLFIAGSSVHDVCQGSLGNCWLVSALAVLAQHPECLKKVIPNVDDQEWHGNRPHSYTGMFRICLWRYGEWLEVVIDDRLPSRNGKLVFVHSKVKNEFWSALIEKAYAKVCGSYESLVGGLTCESMVDFTGGIPEQI-FDLKKYSKSDTEMENLFKLLHFDWKDGSLMCATIA-SRSDEMEEKLANGLITTHAYSITGVCKLRIKSTPMYEHYQKNHLMCIRLRNPWGQKEWNGSLSDASEEWHSISHKDKIAMGWKVENDGEFWMLIDDVLVNFNEITIIRPIKTG----------IFKYNSNWFGSMCKGYWTGVAGDRQSALAEIGQGGQFKIEINNPKGDIFLCHLLQKCARGSEA------ALGILIIAVERNRDFMVHT--PK-----FCNVKLEYRLGGSRSICLRYRLTCGTYIVKPFLTVASPSLKFPIYYLLRCYTTQKNNGTFLPSEMPILERNCICKSNCTIIICRLHXXXXXXXXXXEELDVLNIFCE----IDCENQSLRTTVNCNTSGLFWDETFLLH-RSKMDRPIIIKIFHRGRFFNTLIG 1816
+F NQ Y ++ E +KN LF D F + S+ S + +EWKRPSE+C P LF+ G+ HDV QG LGNCW V+A + LA P L KVIP+ D QEW +P +Y G+FR +R GEW EVV+DD LP+ NGKLV++HSK KNEFWSALIEKAYAK+ G+YE+L G T +++VDFTGG+ E I Y++ + LFK L K+ SLM A+I + +EME++L GLI HAY +T V K+ + + + K H IRLRNPWG+KEWNG SD SEEW + D+ MG ENDGEFWM DD F + T+ I I+K+N NW G G ++ + L + Q+ I + +G+ + L+Q+ R ++ +G I+ VE NR + VH+ PK F N +R + R+ L G Y+V P + P P ++++R +T +++N FL + + + C C C + + ++ +++ C+ I CE + ++T + +T WD L + R I+++I+ ++ +G
Sbjct: 6 YFANQRYSKLKAECEKNEQLFVDSVFKPETSSLYYS-RSCPPEPVEWKRPSEICHPAVPHLFVDGADSHDVTQGKLGNCWFVAACSSLALRPFLLYKVIPDKDKQEWDPKKPENYQGIFRFRFYRQGEWTEVVVDDLLPTVNGKLVYIHSKEKNEFWSALIEKAYAKLAGTYEALAAGSTADALVDFTGGVAEAISLSNGGYAEDQEKRLQLFKTLERGHKEKSLMSASIKINNRNEMEQRLDTGLIKGHAYGVTMVKKVTV-GEGLLSLFNKKHFYMIRLRNPWGEKEWNGRWSDESEEWKKLKPGDRKKMGIVFENDGEFWMEFDDFCREFTDCTLCHVINKAMIHKVFKQWHIYKHNYNWTQGQNAG---GCVQNQDTFL----KNPQYAFSITDNEGEDAMVSLMQEDRRKDKSKGVGNLTIGFYIMKVEENRKYRVHSMFPKAGDSIFIN---------AREVMNRFFLAAGRYLVVP--STYEPHT--PGHFMMRIFTRKRSNAMFLDQDHSVGSKIWCCLPRCRAPQCVV-SVYVTSAAGLQKTGKMSLTCDPYALIICEGRKIKTPIKYDTLIPSWDAGALFYVRQPQKSQIVVQIWDSNWILDSFMG 598
BLAST of Calpain 5a vs. Ensembl Nematostella
Match: EDO40193 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S7W7]) HSP 1 Score: 282.337 bits (721), Expect = 1.263e-84 Identity = 183/500 (36.60%), Postives = 271/500 (54.20%), Query Frame = 2
Query: 92 MRQEHQKNLTLFCDDTFGADDKSISPSGKQEILSSIEWKRPSELCINPCLFIAGSSVHDVCQGSLGNCWLVSALAVLAQHPECLKKVIPNVDDQEWHGN-RPHSYTGMFRICLWRYGEWLEVVIDDRLPSRNGKLVFVHSKVKNEFWSALIEKAYAKVCGSYESLVGGLTCESMVDFTGGIPEQIFDLKKYSKSDTEMENLFKLLHFDWKDGSLMCATIASRSDEMEEKLANGLITTHAYSITGVCKLRIKSTPMYEHYQKNHLMCIRLRNPWG-QKEWNGSLSDASEEWHSISHKDKIAMGWKVENDGEFWMLIDDVLVNFNEITIIRPIKTGIFKYNSNWFGSMCK-GYWTG--VAGDRQSALAEIGQGGQFKIEINNPKGD-----IFLCHLLQKCARGSEA------ALGILIIAVERNRD---------FMVH-----TPKFCNVKLEYRLGGSRSICLRYRLTCGTYIVKPFLTVASPSLKFPIYYLLRCYTTQ 1501
+R++ + F DD F A D SI S + EWKRPS++C +P +F+ G+S D+ QG LG+CWL++A+A L QH + KV+P Q++ N +YTG + C+W+YGEW +V+IDDRLP+ NGKLVFVHS +NEFWSAL+EKAYAK+ GSYESL GG T E++ DFTGG+ E FDL K D LF ++ K +LM +IA++++E+E KL NGL+ HAY++TG +++ K+ + +R+RNPWG ++EW G+ D S EW +S +K ++G E+DGEFWM DD +F + I + +S F S + G W AG ++ + + QF+I + + D + L+QK R + +G I +E +D F H +P F N SR + RY L G Y+V P T +P+ + ++LR Y+ +
Sbjct: 9 VRRQVRSTGKPFEDDEFLAIDSSIFFSKRPP--RPFEWKRPSDICEDPHMFVGGASRLDIQQGMLGDCWLLAAIAALTQHSALMNKVVP----QDYVANPNDRNYTGALKFCIWQYGEWKDVIIDDRLPTYNGKLVFVHSSERNEFWSALLEKAYAKLNGSYESLKGGQTGEALEDFTGGLAES-FDLNKNVPKD-----LFCIMQTAEKRHALMGCSIAAKANEIEAKLNNGLVKGHAYTVTGCRQVKAKT-----RRGDLDVELVRVRNPWGNEREWKGAWGDGSSEWQLLSDYEKKSIGLTFEDDGEFWMSFDDFKKHFTTLEICMLGADSAMEGSSVGFESRSEHGMWRRGLTAGGCRNFIKTFHKNPQFRITLQDSDDDADDLCTVIIALMQKDRRKEKKHGINPLTIGFSIYKLEEEKDKQGNLDMEFFQYHASTARSPVFIN---------SREVVGRYALPPGEYVVIP--TTFNPNEEGN--FVLRMYSEK 478
BLAST of Calpain 5a vs. Ensembl Nematostella
Match: EDO48633 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RJ83]) HSP 1 Score: 234.572 bits (597), Expect = 2.854e-67 Identity = 164/477 (34.38%), Postives = 236/477 (49.48%), Query Frame = 2
Query: 77 QNYVSMRQEHQKNLTLFCDDTFGADDKSISPSGKQEILSSIEWKRPSELCINPCLFIAGSSVHDVCQGSLGNCWLVSALAVLAQHPECLKKVIPNVDDQEWHGNRPHSYTGMFRICLWRYGEWLEVVIDDRLPSRNGKLVFVHSKVKNEFWSALIEKAYAKVCGSYESLVGGLTCESMVDFTGGIPEQIFDLKKYSKSDTEMENLFKLLHFDWKDGSLMCATIASR---SDEMEEKLANGLITTHAYSITGVCKLRIKSTPMYEHYQKNHLMCIRLRNPWGQ-KEWNGSLSDASEEWHSISHKDKIAMGWKVENDGEFWMLIDDVLVNFNEITIIRPIKTGIFKYN-SNWFGSMCKGYWT-GV-AGDRQSALAEIGQGGQFKIEINNP-KGDIFLCHLLQKCAR---------GSEAALGILIIAVER--------NRDFMVHTPKFCNVKLEYRLGGSRSICLRYRLTCGTYIVKP 1432
+ + S++++ + LF D F A ++ + + L WKRP E+ +P G S DV QG + NCW V+ALA L + + L V P DQ + P SY GMFR W++GEW+E+++DD LP+ NG+L F S +EFWSAL+EKAYAK+CGSYE++ G E++ D TGG+ E + DL+ LF ++ ++ SLM +I + SD+ E L NGLIT HAYSITG+ K + S + + IRLRNP EW G+ SD S EW+ +S ++K + ++DGEFWM DD L NF + I + + + W KG W GV AG ++ +A QF++ + NP D LC ++ R A +G I +R F + V + R I RYRL G Y+V P
Sbjct: 10 ERFASLQRQLLNSKRLFEDRDFPASNRVLFTRRRSVQLI---WKRPHEISKSPQFIARGFSSSDVAQGRIRNCWFVAALADLTTNIDLLHHVCP--PDQSFE---PGSYCGMFRFNFWQFGEWVEIIVDDYLPTYNGRLYFTKSDDPDEFWSALLEKAYAKLCGSYEAMSLGRISEALQDMTGGMIENV-DLQ------NPPHGLFNVMMKAYERQSLMGCSIEVKKYSSDQYEGTLENGLITRHAYSITGLRKATLSSL-----RGRPEVDLIRLRNPHANSSEWRGAWSDRSPEWNMLSSQEKRELNLTFDDDGEFWMSFDDFLNNFTRLEICMLTPDSMEQNDRRKWDVQNEKGRWQKGVTAGGCRNFIASFYTNPQFRVCLKNPDDDDDELCTVIVSLMRVDHSHRVKIPDRAPIGFAIYKAPSDMRKGDRLDRAFFEYNKA---VAMTTSFSKLREISSRYRLHAGEYVVIP 463
BLAST of Calpain 5a vs. Ensembl Nematostella
Match: EDO48536 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RJ84]) HSP 1 Score: 203.371 bits (516), Expect = 3.087e-56 Identity = 115/310 (37.10%), Postives = 174/310 (56.13%), Query Frame = 2
Query: 218 ELCINPCLFIAGSSVHDVCQGSLGNCWLVSALAVLAQHPECLKKVIPNVDDQEWHGNR-PHSYTGMFRICLWRYGEWLEVVIDDRLPSRNGKLVFVHSKVKNEFWSALIEKAYAKVCGSYESLVGGLTCESMVDFTGGIPEQIFDLKKYSKSDTEMENLFKLLHFDWKDGSLMCATIASRSDEMEEKLANGLITTHAYSITGVCKLRIKSTPMYEHYQKNHLMCIRLRNPWG-QKEWNGSLSDASEEWHSISHKDKIAMGWKVENDGEFWMLIDDVLVNFNEITI-IRPIKTGIFKYNSNWFGSMCKGYW 1138
E+C P ++ G+S D+ QG LGNCWL++A++VLAQH + +++P + + +R YTG LW+YGEW+ V+IDDRLP+ GKLVFVHS KNEFW AL+EKAYAK+ GSYE+L GGLT E++ DFTGG+ E +F L+ NLF+++ K +LM +I N ++T + + IR+RNPWG +KEW G+ D S EW +S +K ++ + + DGEFWM +D +F+++ + + ++ I K ++ + G+W
Sbjct: 12 EICSEPRFYVDGASRLDIKQGVLGNCWLLAAMSVLAQHKPLVDRIMP---EGVYEADRFSPRYTGKVVFKLWQYGEWVTVIIDDRLPTYKGKLVFVHSPEKNEFWCALLEKAYAKIHGSYEALKGGLTGEALEDFTGGLSE-VFVLEDAPA------NLFQIMQRAEKRSALMGCSI------------NVFLST-----------------------RGGVELIRIRNPWGDEKEWTGAWGDRSPEWQELSQDEKRSLDLEFDVDGEFWMSFEDFKSHFDKLEVCMLDVENAIDKTQISFNSRLENGFW 276
BLAST of Calpain 5a vs. Ensembl Nematostella
Match: EDO37991 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SE78]) HSP 1 Score: 130.954 bits (328), Expect = 1.092e-31 Identity = 102/329 (31.00%), Postives = 157/329 (47.72%), Query Frame = 2
Query: 125 FCDDTFGADDKSISPSGKQ-EILSSIEWKRPSEL-----CIN--PCLFIAGSSVHDVCQGSLGNCWLVSALAVLAQHPECLKKVIPNVDDQEWHGNRPHSYTGMFRICLWRYGEWLEVVIDDRLP-SRNGKLVFVHSKVKNEFWSALIEKAYAKVCGSYESLVGGLTCESMVDFTGGIPEQI-FDLKKYSKSDTE---MENLFKLLHFDWKDGSLMCATIASRSDEMEEKLAN--GLITTHAYSITGVCKLRIKSTPMYEHYQKNHLMCIRLRNPWGQKEWNGSLSDASEEWHSISHKDKIAMGWKVENDGEFWMLIDDVLVNFNEITI 1066
F DD+F KS+ + + E +W RP+E+ C P D+ QG LG+CW +S+LAVLA+ PE L+K++ R S G +++ L + G+W ++IDD LP ++ G+ V+ + + + W LIEKA AK+ GSYE+L G + E + TG E I + + +TE + ++ L G LM A+ + EE N GL T HAYS+ V + + + +RLRNPWGQ W G SDAS W + + G +G FWM ++D + F+ + +
Sbjct: 44 FVDDSFPPVLKSLYINPRHSEHPRVADWLRPNEIDNFSTCRTNIPWAVFRNPRPSDIIQGVLGDCWFLSSLAVLAERPELLEKILI---------TREVSPQGAYQVRLCKDGKWTVILIDDLLPCNQRGQPVYSWAH-RRQLWVPLIEKAMAKLHGSYEALTAGRSIEGLATLTGAPCESIALHESQNNSGNTEPIDKDLIWAQLISSRTAGFLMGASCGGDTQPEEEPGYNAVGLQTHHAYSVLDVRDV-------------HGIRLLRLRNPWGQYSWTGDWSDASSLWTPELRHELMVHG---AQEGVFWMSLEDFMKYFDSVDV 346
BLAST of Calpain 5a vs. Ensembl Medaka
Match: ENSORLT00000002598.2 (calpain-5 [Source:NCBI gene;Acc:101166153]) HSP 1 Score: 355.91 bits (912), Expect = 2.587e-112 Identity = 211/611 (34.53%), Postives = 332/611 (54.34%), Query Frame = 2
Query: 62 VFFNNQNYVSMRQEHQKNLTLFCDDTFGADDKSISPSGKQEILSSIEWKRPSELCINPCLFIAGSSVHDVCQGSLGNCWLVSALAVLAQHPECLKKVIPNVDDQEWHGNRPHSYTGMFRICLWRYGEWLEVVIDDRLPSRNGKLVFVHSKVKNEFWSALIEKAYAKVCGSYESLVGGLTCESMVDFTGGIPEQIFDLKK--YSKSDTEMENLF-KLLHFDWKDGSLMCATIASRSDEMEEKLANGLITTHAYSITGVCKLRIKSTPMYEHYQKNHLMCIRLRNPWGQKEWNGSLSDASEEWHSISHKDKIAMGWKVENDGEFWMLIDDVLVNFNEITIIRPIKTGIFKYNSNWFGSMCKGYWT-----------GVAGDRQSALAEIGQGGQFKIEINNPKGDIFLC------HLLQKCARGSEAALGILIIAVERNRDFMVHTPKFCNVKLEYRLGG-----SRSICLRYRLTCGTYIVKPFLTVASPSLKFPIYYLLRCYTTQKNNGTFLPSEMPILERNCICK-SNCTIIICRLHXXXXXXXXXXEELDVLNIFCEIDCENQSLRTTVNCNTSGLFWDETFLLHRSKMDRPIIIKIFHRGRFFNTLIG 1816
V + Q++ ++R+ Q+ LF D F DKS+ G + + + WKRP EL +P LF+ G S HD+ QG LGNCW V+A + LA +KVIP+ +QEW +P SY G+F WR+GEW++VVIDD LP+ +G+L++ HS NEFWSAL+EKAYAK+ G YE+L GG T +++VDFTGG+ E I DL + Y + D + + LF ++L + G + C+ A+ + +ME +L GL+ HAY++T V ++R+ + ++ + L IR+RNPWGQKEWNG SD+SEEW +S ++ MG V++DGEFWM DD L NF ++ + R I T + W + +G W G + ++ S Q Q+ ++ P+ ++ +C K RG +G I VE NR + +H + ++GG SR++ LR L G Y++ P A +F LLR +T +N L + P R+C ++ ++++ QA+ + + V + + I CE Q +R+ V+ NT +D L +R K +PI ++I++ F ++ +G
Sbjct: 3 VPYEGQSFSALRKRCQQKGCLFEDPLFPPSDKSLFYEGNR--VGQVVWKRPGELSSDPHLFVDGISAHDLHQGQLGNCWFVAACSSLASREALWQKVIPDWKEQEWDKEKPQSYAGIFHFRFWRFGEWVDVVIDDLLPTVSGQLLYGHSNDHNEFWSALVEKAYAKMFGCYEALDGGNTADALVDFTGGVSEPI-DLIQNGYKEDDEKRDELFERVLKVHNRGGLISCSIRATSAADMEARLGCGLVKGHAYAVTDVRRVRL-GHGLLAFFKSDKLSMIRMRNPWGQKEWNGPWSDSSEEWKKVSKSEREKMGVTVDDDGEFWMTFDDFLTNFTDLILCRLINTSYLSLHKTWEEVVVRGSWRRHDEPLRNRAGGCSNNKHSHF----QNPQYVFDVTKPEDEVMVCLQQKDRRATLKEGRGENLPIGFDIQKVELNRVYRMHAA-------QQKVGGSIYINSRAVFLRIDLKKGRYVIVPTTFEAGLEGEF----LLRIFTDVPSNCRELTLDEP--RRSCWSGLRGYPSLVTQVYVQQANGLAGQDSDRVSDPYVIIRCEGQEVRSDVHKNTCSPVFDTKALFYRKKSKQPISVEIYNHNVFKDSFLG 592
BLAST of Calpain 5a vs. Ensembl Medaka
Match: capn5b (calpain 5 [Source:NCBI gene;Acc:101155135]) HSP 1 Score: 337.035 bits (863), Expect = 2.892e-104 Identity = 204/606 (33.66%), Postives = 328/606 (54.13%), Query Frame = 2
Query: 68 FNNQNYVSMRQEHQKNLTLFCDDTFGADDKSISPSGKQEILSSIEWKRPSELCINPCLFIAGSSVHDVCQGSLGNCWLVSALAVLAQHPECLKKVIPNVDDQEWHGNRPHSYTGMFRICLWRYGEWLEVVIDDRLPSRNGKLVFVHSKVKNEFWSALIEKAYAKVCGSYESLVGGLTCESMVDFTGGIPEQIFDLKKYSKSDTEMEN-LF-KLLHFDWKDGSLMCATIASRSDEMEEKLANGLITTHAYSITGVCKLRIKSTPMYEHYQKNHLMCIRLRNPWGQKEWNGSLSDASEEWHSISHKDKIAMGWKVENDGEFWMLIDDVLVNFNEITIIRPIKTGIFKYNSNWFGSMCKGYWT-------GVAGDRQSALAEIGQGGQFKIEINNPKGDIFLC------HLLQKCARGSEAALGILIIAVERNRDFMVHTPKFCNVKLEYRLGG-----SRSICLRYRLTCGTYIVKPFLTVASPSLKFPIYYLLRCYTTQKNNGTFLPSEMPILERNC---ICKSNCTIIICRLHXXXXXXXXXXEELDVLNIFCEIDCENQSLRTTVNCNTSGLFWDETFLLHRSKMDRPIIIKIFHRGRFFNTLIG 1816
F+ Q Y +++++ ++ LF D F A D S+ G + + + WKRP ELC +P LF+ G S HD+ QG LGNCW V+A + LA +KVIP+ +QEW +P SYTG+F WR+GEW++VVIDDRLP+ + +LV+ HS NEFWSAL+EKAYAKV G YE+L GG T +++VDFTGG+ E + L+ +D N LF ++L +DG + C+ A+ ++ME +L GL+ HAY++T V K+R+ + ++ L IR+RNPWG+KEW+G SD+SEEW+ +S ++ +G V++DGEFWM DD F ++ + R I T + W + +G W AG + + Q Q+ ++ + ++ +C K +G A+G I VE NR + +HT + ++ G SR + LR + G Y++ P T P + +LLR +T ++ L + P + C +C ++ ++H++ A+ +Q + ++ + I CE + +R+ V +T +D L +R K I I++++R +T +G
Sbjct: 80 FDGQQYSTLKRQCLQSGLLFEDPRFPAIDDSLFYLGNR--IGRVVWKRPRELCEDPHLFVDGISAHDLHQGQLGNCWFVAACSSLASRESLWQKVIPDWKEQEWDTEKPDSYTGIFHFRFWRFGEWVDVVIDDRLPTVDNQLVYCHSNDSNEFWSALVEKAYAKVYGCYEALDGGNTADALVDFTGGVSEPVDLLEGQMATDEAARNQLFERVLKVHNRDGLISCSIRATTVEDMEARLDCGLVKGHAYAVTDVRKVRLGHG-LLAFFKSEKLQMIRMRNPWGEKEWSGPWSDSSEEWNKVSKSEREKLGVTVQDDGEFWMTFDDFCQYFTDLILCRLINTSYLSIHKTWEEEVIRGLWVHRQDPLRNRAGGCINHKSTFLQNPQYVFDVKKLEDEVLICLQQKERRATPKEGKGENLAIGFDIHRVELNRKYRMHT-------AQQKVAGSIYINSRCVFLRKEMKEGRYVIIP--TTFDPGQQGE--FLLRVFTDVPSDCKELTLDEP--PQTCWTGMC--GYPQLVTQVHVLNAEGLQGQDSNGAVDSYVVITCEGERVRSPVQKDTRCPNFDFKGLFYRKKPKEGIHIEVYNRNMIVDTFLG 667
BLAST of Calpain 5a vs. Ensembl Medaka
Match: ENSORLT00000039414.1 (calpain-5 [Source:NCBI gene;Acc:101158220]) HSP 1 Score: 327.02 bits (837), Expect = 2.995e-101 Identity = 197/618 (31.88%), Postives = 318/618 (51.46%), Query Frame = 2
Query: 47 FSRNFVFFNNQNYVSMRQEHQKNLTLFCDDTFGADDKSI----SPSGKQEILSSIEWKRPSELCINPCLFIAGSSVHDVCQGSLGNCWLVSALAVLAQHPECLKKVIPNVDDQEWHGNRPHSYTGMFRICLWRYGEWLEVVIDDRLPSRNGKLVFVHSKVKNEFWSALIEKAYAKVCGSYESLVGGLTCESMVDFTGGIPEQI-FDLKKYSKSDTEMENLFKLLHFDWKDGSLMCATIASRSDEMEEKLANGLITTHAYSITGVCKLRIKSTPMYEHYQKNHLMCIRLRNPWGQKEWNGSLSDASEEWHSISHKDKIAMGWKVENDGEFWMLIDDVLVNFNEITIIRPIKTGIFKYNSNWFGSMCKGYWT----------GVAGDRQSALAEIGQGGQFKIEINNPKGDIFLC------HLLQKCARGSEAALGILIIAVERNRDFMVHTPKFCNVKLEYRLGGSRSICLRYRLTCGTYIVKPFLTVASPSLKFPIYYLLRCYTTQKNNGTFLPSEMPILERNCICKSNC-TIIICRLHXXXXXXXXXXEELD------VLNIFCEIDCENQSLRTTVNCNTSGLFWDETFLLHRSKMDRPIIIKIFHRGRFFNTLIG 1816
S + V F NQ Y +++E + LF D F ADD S+ P GK +EWKRP ++ +P LF+ G + HD+ QG +GNCW V+A + LA P +KVIP+ +QEW P +Y G+F W +G+W +VV+DDRLP+ NGKL++ HS K EFWSAL+EKAYAK+ G YESL GG +++VDFTG + E+I + + + K + LF+ L ++ G ++ +I ++ DE+E K+ANGL+ HAYS+T V ++ + + H++ L IR+RNPWG+ EWNG+ SD+SEEW I ++ +G VE+DGEFWM +D F + + I T + + W + G WT G G+ + L Q Q+ ++ ++ + +K +G ++G ++ VE NR + +H +RS+ +R L G YI+ P A +F +LR +T + L + P K C T + + ++ AE L+ + + I CE +S+++ V+ +T +D + + +R K +PI +++++ + +G
Sbjct: 1 MSSSVVPFKNQRYADLKRESIQRKILFRDPEFPADDSSLFYKEPPPGK------VEWKRPWKISSDPHLFVEGINSHDLYQGEIGNCWFVAASSCLAMMPHLWQKVIPDWQEQEWDPKHPENYAGIFHFQFWVFGQWTDVVVDDRLPTINGKLIYCHSNSKKEFWSALLEKAYAKLYGCYESLSGGFAGDAVVDFTGAVYERIDLEREAFYKDQRNQDYLFENLLKVYEHGGIISCSIKAKPDELEFKMANGLVKGHAYSVTAVKRIHL-CHELVAHFKTETLPMIRMRNPWGKMEWNGAWSDSSEEWSKIGDTERHNLGLTVEDDGEFWMSFEDWCKKFTDADVCSIINTSLVSIHKTWSEVLHFGSWTKHGDPLLNRCGGCGNNNTFL----QNPQYMFDVTKEIEEVLITLQQKDRRTFKKTGQGENLSIGFSVLKVELNRKYRIHRLLSQKSMESSVFINARSVFVRCILPQGRYIIIPSTFQAGELGEF----MLRIFTDVDSGCRELTEDKP--------KVKCWTPLFGYPQAVTHVFVEKAERLENQDRTAGADPYVIISCEGKSVKSIVHKDTLNPQFDTSAVFYRKKPSKPITVEVWNSNAVADEFMG 595
BLAST of Calpain 5a vs. Ensembl Medaka
Match: capn5b (calpain 5 [Source:NCBI gene;Acc:101155135]) HSP 1 Score: 328.946 bits (842), Expect = 4.680e-101 Identity = 202/622 (32.48%), Postives = 321/622 (51.61%), Query Frame = 2
Query: 68 FNNQNYVSMRQEHQKNLTLFCDDTFGADDKSISPSGKQEILSSIEWKRPSELCINPCLFIAGSSVHDVCQGSLGNCWLVSALAVLAQHPECLKKVIPNVDDQEWHGNRPHSYTGMFRICLWRYGEWLEVVIDDRLPSRNGKLVFVHSKVKNEFWSALIEKAYAKVCGSYESLVGGLTCESMVDFTGGIPEQIFDLKKYSKSDTEMEN-LF-KLLHFDWKDGSLMCATIASRSDEMEEKLANGLITTHAYSITGVCKLRIKSTPMYEHYQKNHLMCIRLRNPWGQKEWNGSLSDASEEWHSISHKDKIAMGWKVENDGEFWMLIDDVLVNFNEITIIRPIKTGIFKYNSNWFGSMCKGYWT-------GVAGDRQSALAEIGQGGQFKIEINNPKGDIFLC------HLLQKCARGSEAALGILIIAVERNRDFMVHTPKFCNVKLEYRLGG-----SRSICLRYRLTCGTYIVKPFLTVASPSLKFPIYYLLRCYTTQKNNGTFLPSEMP-------------------ILERNCICKSNCTIIICRLHXXXXXXXXXXEELDVLNIFCEIDCENQSLRTTVNCNTSGLFWDETFLLHRSKMDRPIIIKIFHRGRFFNTLIG 1816
F+ Q Y +++++ ++ LF D F A D S+ G + + + WKRP ELC +P LF+ G S HD+ QG LGNCW V+A + LA +KVIP+ +QEW +P SYTG+F WR+GEW++VVIDDRLP+ + +LV+ HS NEFWSAL+EKAYAKV G YE+L GG T +++VDFTGG+ E + L+ +D N LF ++L +DG + C+ A+ ++ME +L GL+ HAY++T V K+R+ + ++ L IR+RNPWG+KEW+G SD+SEEW+ +S ++ +G V++DGEFWM DD F ++ + R I T + W + +G W AG + + Q Q+ ++ + ++ +C K +G A+G I VE NR + +HT + ++ G SR + LR + G Y++ P T P + +LLR +T ++ L + P +L + + LH + + + ++ + I CE + +R+ V +T +D L +R K I I++++R +T +G
Sbjct: 80 FDGQQYSTLKRQCLQSGLLFEDPRFPAIDDSLFYLGNR--IGRVVWKRPRELCEDPHLFVDGISAHDLHQGQLGNCWFVAACSSLASRESLWQKVIPDWKEQEWDTEKPDSYTGIFHFRFWRFGEWVDVVIDDRLPTVDNQLVYCHSNDSNEFWSALVEKAYAKVYGCYEALDGGNTADALVDFTGGVSEPVDLLEGQMATDEAARNQLFERVLKVHNRDGLISCSIRATTVEDMEARLDCGLVKGHAYAVTDVRKVRLGHG-LLAFFKSEKLQMIRMRNPWGEKEWSGPWSDSSEEWNKVSKSEREKLGVTVQDDGEFWMTFDDFCQYFTDLILCRLINTSYLSIHKTWEEEVIRGLWVHRQDPLRNRAGGCINHKSTFLQNPQYVFDVKKLEDEVLICLQQKERRATPKEGKGENLAIGFDIHRVELNRKYRMHT-------AQQKVAGSIYINSRCVFLRKEMKEGRYVIIP--TTFDPGQQGE--FLLRVFTDVPSDCKELTLDEPPQTCWTGMCGYPQLVTQVHVLNAEGLQGQDSN---GELHCLNPQKKRLLGKAQAVDSYVVITCEGERVRSPVQKDTRCPNFDFKGLFYRKKPKEGIHIEVYNRNMIVDTFLG 684
BLAST of Calpain 5a vs. Ensembl Medaka
Match: ENSORLT00000028657.1 (calpain-5 [Source:NCBI gene;Acc:101158220]) HSP 1 Score: 322.783 bits (826), Expect = 9.791e-100 Identity = 195/617 (31.60%), Postives = 315/617 (51.05%), Query Frame = 2
Query: 50 SRNFVFFNNQNYVSMRQEHQKNLTLFCDDTFGADDKSI----SPSGKQEILSSIEWKRPSELCINPCLFIAGSSVHDVCQGSLGNCWLVSALAVLAQHPECLKKVIPNVDDQEWHGNRPHSYTGMFRICLWRYGEWLEVVIDDRLPSRNGKLVFVHSKVKNEFWSALIEKAYAKVCGSYESLVGGLTCESMVDFTGGIPEQI-FDLKKYSKSDTEMENLFKLLHFDWKDGSLMCATIASRSDEMEEKLANGLITTHAYSITGVCKLRIKSTPMYEHYQKNHLMCIRLRNPWGQKEWNGSLSDASEEWHSISHKDKIAMGWKVENDGEFWMLIDDVLVNFNEITIIRPIKTGIFKYNSNWFGSMCKGYWT----------GVAGDRQSALAEIGQGGQFKIEINNPKGDIFLC------HLLQKCARGSEAALGILIIAVERNRDFMVHTPKFCNVKLEYRLGGSRSICLRYRLTCGTYIVKPFLTVASPSLKFPIYYLLRCYTTQKNNGTFLPSEMPILERNCICKSNC-TIIICRLHXXXXXXXXXXEELD------VLNIFCEIDCENQSLRTTVNCNTSGLFWDETFLLHRSKMDRPIIIKIFHRGRFFNTLIG 1816
S + V F NQ Y +++E + LF D F ADD S+ P GK +EWKRP ++ +P LF+ G + HD+ QG +GNCW V+A + LA P +KVIP+ +QEW P +Y G+F W +G+W +VV+DDRLP+ NGKL++ HS K EFWSAL+EKAYAK+ G YESL GG +++VDFTG + E+I + + + K + LF+ L ++ G ++ I + E+E K+ANGL+ HAYS+T V ++ ++ + H++ + IR+RNPWG+ EWNG SD+SEEW I ++ +G VE+DGEFWM +D F + + I T + + W + G WT G G+ + L Q Q+ ++ ++ + +K +G ++G ++ VE NR + +H +RS+ +R L G YI+ P A +F +LR +T + L + P K C T + + ++ AE L+ + + I CE +S+++ V+ +T +D + + +R K +PI +++++ + +G
Sbjct: 2 SSSVVPFKNQRYADLKRESIQRKILFRDPEFPADDSSLFYKEPPPGK------VEWKRPWKISSDPHLFVEGINSHDLYQGEIGNCWFVAASSCLAMMPHLWQKVIPDWQEQEWDPKHPENYAGIFHFQFWVFGQWTDVVVDDRLPTINGKLIYCHSNSKKEFWSALLEKAYAKLYGCYESLSGGFAGDAVVDFTGAVYERIDLEREAFYKDQRNQDYLFENLLKVYEHGGIISCGITAEPHELEFKMANGLVKGHAYSVTAVKRIHLRHE-LVAHFKTETIPMIRMRNPWGKMEWNGPWSDSSEEWSKIGDTERHNLGLTVEDDGEFWMSFEDWCKKFTDADVCSIINTSLVSIHKTWSEVLHFGSWTKHGDPLLNRCGGCGNNNTFL----QNPQYMFDVTKEIEEVLITLQQKDRRTFKKTGQGENLSIGFSVLKVELNRKYRIHRLLSQKSMESSVFINARSVFVRCILPQGRYIIIPSTFQAGELGEF----MLRIFTDVDSGCRELTEDKP--------KVKCWTPLFGYPQAVTHVFVEKAERLENQDRTAGADPYVIISCEGKSVKSIVHKDTLNPQFDTSAVFYRKKPSKPITVEVWNSNAVADEFMG 595
BLAST of Calpain 5a vs. Planmine SMEST
Match: SMESG000009459.1 (SMESG000009459.1) HSP 1 Score: 1282.7 bits (3318), Expect = 0.000e+0 Identity = 626/626 (100.00%), Postives = 626/626 (100.00%), Query Frame = 2
Query: 92 MRQEHQKNLTLFCDDTFGADDKSISPSGKQEILSSIEWKRPSELCINPCLFIAGSSVHDVCQGSLGNCWLVSALAVLAQHPECLKKVIPNVDDQEWHGNRPHSYTGMFRICLWRYGEWLEVVIDDRLPSRNGKLVFVHSKVKNEFWSALIEKAYAKVCGSYESLVGGLTCESMVDFTGGIPEQIFDLKKYSKSDTEMENLFKLLHFDWKDGSLMCATIASRSDEMEEKLANGLITTHAYSITGVCKLRIKSTPMYEHYQKNHLMCIRLRNPWGQKEWNGSLSDASEEWHSISHKDKIAMGWKVENDGEFWMLIDDVLVNFNEITIIRPIKTGIFKYNSNWFGSMCKGYWTGVAGDRQSALAEIGQGGQFKIEINNPKGDIFLCHLLQKCARGSEAALGILIIAVERNRDFMVHTPKFCNVKLEYRLGGSRSICLRYRLTCGTYIVKPFLTVASPSLKFPIYYLLRCYTTQKNNGTFLPSEMPILERNCICKSNCTIIICRLHXXXXXXXXXXEELDVLNIFCEIDCENQSLRTTVNCNTSGLFWDETFLLHRSKMDRPIIIKIFHRGRFFNTLIGYSCCSYDSRHLNSPEVIETLNMVFEPKLKTPCTKIRNLKITQFHTDNQKYL 1969
MRQEHQKNLTLFCDDTFGADDKSISPSGKQEILSSIEWKRPSELCINPCLFIAGSSVHDVCQGSLGNCWLVSALAVLAQHPECLKKVIPNVDDQEWHGNRPHSYTGMFRICLWRYGEWLEVVIDDRLPSRNGKLVFVHSKVKNEFWSALIEKAYAKVCGSYESLVGGLTCESMVDFTGGIPEQIFDLKKYSKSDTEMENLFKLLHFDWKDGSLMCATIASRSDEMEEKLANGLITTHAYSITGVCKLRIKSTPMYEHYQKNHLMCIRLRNPWGQKEWNGSLSDASEEWHSISHKDKIAMGWKVENDGEFWMLIDDVLVNFNEITIIRPIKTGIFKYNSNWFGSMCKGYWTGVAGDRQSALAEIGQGGQFKIEINNPKGDIFLCHLLQKCARGSEAALGILIIAVERNRDFMVHTPKFCNVKLEYRLGGSRSICLRYRLTCGTYIVKPFLTVASPSLKFPIYYLLRCYTTQKNNGTFLPSEMPILERNCICKSNCTIIICRLHIIQADIIQAAEELDVLNIFCEIDCENQSLRTTVNCNTSGLFWDETFLLHRSKMDRPIIIKIFHRGRFFNTLIGYSCCSYDSRHLNSPEVIETLNMVFEPKLKTPCTKIRNLKITQFHTDNQKYL
Sbjct: 1 MRQEHQKNLTLFCDDTFGADDKSISPSGKQEILSSIEWKRPSELCINPCLFIAGSSVHDVCQGSLGNCWLVSALAVLAQHPECLKKVIPNVDDQEWHGNRPHSYTGMFRICLWRYGEWLEVVIDDRLPSRNGKLVFVHSKVKNEFWSALIEKAYAKVCGSYESLVGGLTCESMVDFTGGIPEQIFDLKKYSKSDTEMENLFKLLHFDWKDGSLMCATIASRSDEMEEKLANGLITTHAYSITGVCKLRIKSTPMYEHYQKNHLMCIRLRNPWGQKEWNGSLSDASEEWHSISHKDKIAMGWKVENDGEFWMLIDDVLVNFNEITIIRPIKTGIFKYNSNWFGSMCKGYWTGVAGDRQSALAEIGQGGQFKIEINNPKGDIFLCHLLQKCARGSEAALGILIIAVERNRDFMVHTPKFCNVKLEYRLGGSRSICLRYRLTCGTYIVKPFLTVASPSLKFPIYYLLRCYTTQKNNGTFLPSEMPILERNCICKSNCTIIICRLHIIQADIIQAAEELDVLNIFCEIDCENQSLRTTVNCNTSGLFWDETFLLHRSKMDRPIIIKIFHRGRFFNTLIGYSCCSYDSRHLNSPEVIETLNMVFEPKLKTPCTKIRNLKITQFHTDNQKYL 626
BLAST of Calpain 5a vs. Planmine SMEST
Match: SMESG000009459.1 (SMESG000009459.1) HSP 1 Score: 661.759 bits (1706), Expect = 0.000e+0 Identity = 328/328 (100.00%), Postives = 328/328 (100.00%), Query Frame = 2
Query: 986 MGWKVENDGEFWMLIDDVLVNFNEITIIRPIKTGIFKYNSNWFGSMCKGYWTGVAGDRQSALAEIGQGGQFKIEINNPKGDIFLCHLLQKCARGSEAALGILIIAVERNRDFMVHTPKFCNVKLEYRLGGSRSICLRYRLTCGTYIVKPFLTVASPSLKFPIYYLLRCYTTQKNNGTFLPSEMPILERNCICKSNCTIIICRLHXXXXXXXXXXEELDVLNIFCEIDCENQSLRTTVNCNTSGLFWDETFLLHRSKMDRPIIIKIFHRGRFFNTLIGYSCCSYDSRHLNSPEVIETLNMVFEPKLKTPCTKIRNLKITQFHTDNQKYL 1969
MGWKVENDGEFWMLIDDVLVNFNEITIIRPIKTGIFKYNSNWFGSMCKGYWTGVAGDRQSALAEIGQGGQFKIEINNPKGDIFLCHLLQKCARGSEAALGILIIAVERNRDFMVHTPKFCNVKLEYRLGGSRSICLRYRLTCGTYIVKPFLTVASPSLKFPIYYLLRCYTTQKNNGTFLPSEMPILERNCICKSNCTIIICRLHIIQADIIQAAEELDVLNIFCEIDCENQSLRTTVNCNTSGLFWDETFLLHRSKMDRPIIIKIFHRGRFFNTLIGYSCCSYDSRHLNSPEVIETLNMVFEPKLKTPCTKIRNLKITQFHTDNQKYL
Sbjct: 1 MGWKVENDGEFWMLIDDVLVNFNEITIIRPIKTGIFKYNSNWFGSMCKGYWTGVAGDRQSALAEIGQGGQFKIEINNPKGDIFLCHLLQKCARGSEAALGILIIAVERNRDFMVHTPKFCNVKLEYRLGGSRSICLRYRLTCGTYIVKPFLTVASPSLKFPIYYLLRCYTTQKNNGTFLPSEMPILERNCICKSNCTIIICRLHIIQADIIQAAEELDVLNIFCEIDCENQSLRTTVNCNTSGLFWDETFLLHRSKMDRPIIIKIFHRGRFFNTLIGYSCCSYDSRHLNSPEVIETLNMVFEPKLKTPCTKIRNLKITQFHTDNQKYL 328
BLAST of Calpain 5a vs. Planmine SMEST
Match: SMESG000017238.1 (SMESG000017238.1) HSP 1 Score: 299.286 bits (765), Expect = 9.558e-90 Identity = 189/527 (35.86%), Postives = 273/527 (51.80%), Query Frame = 2
Query: 77 QNYVSMRQEHQKNLTLFCDDTFGADDKSISPSGKQEILSSIEWKRPSELCINPCLFIAGSSVHDVCQGSLGNCWLVSALAVLAQHPECLKKVIPNVDDQEWHGNRPHSYTGMFRICLWRYGEWLEVVIDDRLPSRNGKLVFVHSKVKNEFWSALIEKAYAKVCGSYESLVGGLTCESMVDFTGGIPEQIFDLKKYSKSDTE-MENLFKLLHFDWKDGSLMCATIASRSDEMEEKLANGLITTHAYSITGVCKLRIKSTPMYEHYQKNHLMCIRLRNPWGQKEWNGSLSDASEEWHSISHKDKIAMGWKVENDGEFWMLIDDVLVNFNEITI--IRPIKTG-IFKYNSN---WFGSMCKGYWT--GVAGDRQSALAEIGQGGQFKIEINNPKGD------IFLCHLLQKCARG------------------SEAALGILIIAVERNRDFMVHTPKFCNVKLEYRLGGSRSICLRYRLTCGTYIVKPFLTVASPSLKFPIYYLLRCYTTQKNNGTFLPSEMPILERNCI 1558
++Y S++++ TL+ D+ F A+ +SI + + + +WKRP E+C +P F+ G+S DVCQG LG+CWL++A+A L+ +V PN DQ + + Y G F W YGEW E++IDDRLP+RN +LVF+HS+ NEFWSAL+EKAYAK+CGSYE L GG + E+M DFTGGI E I DLK ++ + E E LF+++ SLM +I + E KL NGLI HAYSIT V ++ K Y + + +R+RNPWGQ EWNG D+S+ WH IS K++ +G +NDGEFWM +D + NF ++ I + P G NSN W S+ +G W AG + QF+I + +P D + LLQK R G L R + +P F N+ R +C R+RL G+Y + P + F LLR ++ + N G + + ++N +
Sbjct: 90 ESYDSLKEKLVAKGTLYEDEDFPANGESIYFTSNKG--GNFQWKRPHEICSDPQFFVGGASRFDVCQGELGDCWLLAAVASLSMQTMLFSQVAPN--DQSFAKGK---YCGAFHFNFWHYGEWKEIIIDDRLPTRNNRLVFMHSRDSNEFWSALLEKAYAKLCGSYELLKGGSSSEAMEDFTGGITE-IIDLKGDNRKNNESKEKLFQIMIQSHSKMSLMGCSIDANPKVTEAKLGNGLIMGHAYSITAVKQISAK----VPGYNGDIIKLVRIRNPWGQSEWNGEWGDSSDSWHHISDKERGVLGLNFDNDGEFWMSFNDFVNNFEKLEICHLGPQSVGESLLKNSNKRTWESSVQQGAWKKGASAGGCLNFQETFWSNPQFRIVVTDPDKDDTDNMGTVIIGLLQKNMRSIKNRGNDLFPIGYVLYKLPSTDCGPLDKVFFRTNRKVAMSPAFINM---------REVCGRHRLPPGSYAIIPSTFEVNQEADF----LLRIFSERANCGKEIDETSKMDDKNLV 591
BLAST of Calpain 5a vs. Planmine SMEST
Match: SMESG000062902.1 (SMESG000062902.1) HSP 1 Score: 265.003 bits (676), Expect = 2.470e-77 Identity = 181/508 (35.63%), Postives = 263/508 (51.77%), Query Frame = 2
Query: 80 NYVSMRQEHQKNLTLFCDDTFGADDKSISPSGKQEILSSIEWKRPSELCINPCLFIAGSSVHDVCQGSLGNCWLVSALAVLAQHPECLKKVIPNVDDQEWHGNRPHSYTGMFRICLWRYGEWLEVVIDDRLPSRNGKLVFVHSKVKNEFWSALIEKAYAKVCGSYESLVGGLTCESMVDFTGGIPEQIFDLKKYSKSDTEMENLFKLLHFDWKDGSLMCATIASRSDEMEEKLANGLITTHAYSITGVCKLRIKSTPMYEHYQKNHLMCIRLRNPWG-QKEWNGSLSDASEEWHSISHKDKIAMGWKVENDGEFWMLIDDVLVNFNEITIIRPIKTGIFKYNSNWFG----SMC--KGYWTG--VAGDRQSALAEIGQGGQFKIEINNPKGD------IFLCHLLQKCARGSEAALGILIIAVE-----------RNRDF------MVHTPKFCNVKLEYRLGGSRSICLRYRLTCGTYIVKPFLTVASPSLKFPIYYLLRCYTTQKN 1507
+Y + ++ + L+ D F AD S+ S + EWKRP ++C NP LF G S DV QG+LG+CWL++ +A L+ + K V+P DQ++ N Y G FR WR+G+W+EVV+DDRLP+ GKLVF+HS NEFWSAL+EKAYAK+CG YESL GG E+M DFTGG+ E I K ++ + + ++L F K +LM I S ++E +ANGLI+ HAYSIT V K++ + E +R+RNPWG +KEW G SD+S EW +IS +K + NDGEFWM DD NF +I I G N + G MC G W AG ++ + QF++E+ +P + + L+QK R + LG L+I N+ F + +P F N R +C R++L G Y++ P + +P+ + ++LR ++ + N
Sbjct: 51 SYSDLLEQLKGTGKLWEDPDFPADTSSVFVSKASKGYREFEWKRPKDICKNPQLFADGVSRFDVQQGALGDCWLLAVVASLSLYEPIFKNVVPT--DQDFDKN----YIGAFRFRFWRFGKWVEVVVDDRLPTYGGKLVFMHSADNNEFWSALLEKAYAKMCGIYESLSGGSQIEAMEDFTGGLCETI----KLNEKERPVGLYNQMLKFA-KQSTLMGCAIESA--KIEGVMANGLISGHAYSITDVQKVKQTDGTVVE--------LVRVRNPWGNEKEWKGKWSDSSPEWETISKSEKERLCSNFANDGEFWMTFDDFAKNFTKIEICH---LGPESLNEDLSGKRRWEMCIEDGSWCKNINAGGCRNFIDSFVTNPQFRVEVVDPDDEDSENTGTVIIGLMQKDMR--QRNLGFLVIGYVIYKLKDPNCGLLNKSFFKFSTTVAKSPGFIN---------QREVCGRHKLPTGHYVIIP--STFNPNEE--ANFMLRIFSEKPN 519
BLAST of Calpain 5a vs. Planmine SMEST
Match: SMESG000062904.1 (SMESG000062904.1) HSP 1 Score: 263.077 bits (671), Expect = 9.407e-77 Identity = 182/508 (35.83%), Postives = 263/508 (51.77%), Query Frame = 2
Query: 101 EHQKNL-TLFCDDTFGADDKSISPSGKQEILSSIEWKRPSELCINPCLFIAGSSVHDVCQGSLGNCWLVSALAVLAQHPECLKKVIPNVDDQEWHGNRPHSYTGMFRICLWRYGEWLEVVIDDRLPSRNGKLVFVHSKVKNEFWSALIEKAYAKVCGSYESLVGGLTCESMVDFTGGIPEQIFDLKKYSKSDTEMENLFKLLHFDWKDGSLMCATIASRSDEMEEKLANGLITTHAYSITGVCKLRIKSTPMYEHYQKNHLMCIRLRNPWG-QKEWNGSLSDASEEWHSISHKDKIAMGWKVENDGEFWMLIDDVLVNFNEITIIRPIKTGIFKYNSNWFG----SMC--KGYWTG--VAGDRQSALAEIGQGGQFKIEINNPKGD------IFLCHLLQKCARGSE---AALGILIIAVER------NRDF------MVHTPKFCNVKLEYRLGGSRSICLRYRLTCGTYIVKPFLTVASPSLKFPIYYLLRCYTTQKNNGTFLPSE 1531
EH K L+ D F AD S+ S + EWKRP ++C NP LF G S DV QG+LG+CWL++ +A L+ + K V+P DQ++ N Y G FR WR+G+W+EVV+DDRLP+ G+LVF+HS NEFWSAL+EKAYAK+CG YESL GG E+M DFTGG+ E I K ++ + + ++L F K +LM I S ++E +ANGLI+ HAYSIT V K++ + E +R+RNPWG +KEW G SD+S EW +IS +K + NDGEFWM DD NF +I I G N + G MC G W AG ++ + QF++E+ +P + + L+QK R +G +I ++ N+ F + +P F N R +C R++L G Y++ P + +P+ + ++LR ++ ++N L E
Sbjct: 57 EHLKGTGKLWEDPDFPADTSSVFVSKASKGYREFEWKRPKDICKNPQLFADGVSRFDVQQGALGDCWLLAVVASLSLYEPIFKNVVPT--DQDFDKN----YIGAFRFRFWRFGKWVEVVVDDRLPTYRGRLVFMHSADNNEFWSALLEKAYAKMCGIYESLNGGSQIEAMEDFTGGLCETI----KLNEKERPVGLYNQMLKFA-KQSTLMGCAIESA--KIEGVMANGLISGHAYSITDVQKVKQTDGTVVE--------LVRVRNPWGNEKEWKGKWSDSSPEWETISKSEKERLCSNFANDGEFWMTFDDFAKNFTKIEICH---LGPESLNEDLSGKRRWEMCIEDGSWCKNINAGGCRNFIDSFVTNPQFRVEVVDPDDEDSEDTGTVIIGLMQKDMRQRNLDFLVIGYVIYKLKDPNCGLLNKSFFKFSTTVAKSPGFIN---------QREVCGRHKLPTGHYVIIP--STFNPNEE--ANFMLRIFSEKQNKSKELDVE 527 The following BLAST results are available for this feature:
BLAST of Calpain 5a vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of Calpain 5a vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 5
BLAST of Calpain 5a vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 5
BLAST of Calpain 5a vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of Calpain 5a vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of Calpain 5a vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of Calpain 5a vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of Calpain 5a vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of Calpain 5a vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of Calpain 5a vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 5
BLAST of Calpain 5a vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of Calpain 5a vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 5
BLAST of Calpain 5a vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of Calpain 5a vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30023857 ID=SMED30023857|Name=Calpain 5a|organism=Schmidtea mediterranea sexual|type=transcript|length=2338bpback to top protein sequence of SMED30023857-orf-1 >SMED30023857-orf-1 ID=SMED30023857-orf-1|Name=SMED30023857-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=644bp MDFSRNFVFFNNQNYVSMRQEHQKNLTLFCDDTFGADDKSISPSGKQEILback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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