LITAF domain-containing protein

Overview
NameLITAF domain-containing protein
Smed IDSMED30023825
Length (bp)600
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of LITAF domain-containing protein (SMED30023825) t-SNE clustered cells

Violin plots show distribution of expression levels for LITAF domain-containing protein (SMED30023825) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of LITAF domain-containing protein (SMED30023825) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for LITAF domain-containing protein (SMED30023825) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30023825

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 14

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
Smed sexual biotypeSMED30023825h1SMcG0015128 Contig55623uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30023825h1SMcG0015128 Contig55623newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
pharynxSMED30023825h1SMcG0015128 dd_Smed_v4_353_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
protonephridiaSMED30023825h1SMcG0015128 dd_Smed_v4_353_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30023825h1SMcG0015128 dd_Smed_v4_353_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30023825h1SMcG0015128 dd_Smed_v4_353_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30023825h1SMcG0015128 dd_Smed_v4_353_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30023825h1SMcG0015128 dd_Smed_v4_353_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30023825h1SMcG0015128 dd_Smed_v4_353_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parapharyngeal regionSMED30023825h1SMcG0015128 dd_Smed_v4_353_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30023825h1SMcG0015128 dd_Smed_v4_353_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymaSMED30023825h1SMcG0015128 dd_Smed_v4_353_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30023825h1SMcG0015128 dd_Smed_v4_353_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30023825h1SMcG0015128 dd_Smed_v4_353_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of LITAF domain-containing protein vs. Ensembl Human
Match: CDIP1 (cell death inducing p53 target 1 [Source:HGNC Symbol;Acc:HGNC:13234])

HSP 1 Score: 49.2914 bits (116), Expect = 2.271e-7
Identity = 36/81 (44.44%), Postives = 52/81 (64.20%), Query Frame = 2 
Query:  209 IIQNQVYGCLPVNTVCPHCQAQIVTGIRYDNXXXXXXXXXXXXXXXXXXXXXXIPFCVDDCKDVIHVCPSCNMKVGEHRRV 451
            ++Q +++   PV TVCPHCQ  I T I Y+ GL+ ++L      +GC LGCCLIP  ++D KDV H CPSC   +  ++R+
Sbjct:   48 VLQGEIFEGAPVQTVCPHCQQAITTKISYEIGLMNFVLGFFCCFMGCDLGCCLIPCLINDFKDVTHTCPSCKAYIYTYKRL 128
[Close]
BLAST of LITAF domain-containing protein vs. Ensembl Human
Match: CDIP1 (cell death inducing p53 target 1 [Source:HGNC Symbol;Acc:HGNC:13234])

HSP 1 Score: 49.2914 bits (116), Expect = 2.271e-7
Identity = 36/81 (44.44%), Postives = 52/81 (64.20%), Query Frame = 2 
Query:  209 IIQNQVYGCLPVNTVCPHCQAQIVTGIRYDNXXXXXXXXXXXXXXXXXXXXXXIPFCVDDCKDVIHVCPSCNMKVGEHRRV 451
            ++Q +++   PV TVCPHCQ  I T I Y+ GL+ ++L      +GC LGCCLIP  ++D KDV H CPSC   +  ++R+
Sbjct:   48 VLQGEIFEGAPVQTVCPHCQQAITTKISYEIGLMNFVLGFFCCFMGCDLGCCLIPCLINDFKDVTHTCPSCKAYIYTYKRL 128
[Close]
BLAST of LITAF domain-containing protein vs. Ensembl Human
Match: CDIP1 (cell death inducing p53 target 1 [Source:HGNC Symbol;Acc:HGNC:13234])

HSP 1 Score: 48.9062 bits (115), Expect = 5.803e-7
Identity = 36/81 (44.44%), Postives = 52/81 (64.20%), Query Frame = 2 
Query:  209 IIQNQVYGCLPVNTVCPHCQAQIVTGIRYDNXXXXXXXXXXXXXXXXXXXXXXIPFCVDDCKDVIHVCPSCNMKVGEHRRV 451
            ++Q +++   PV TVCPHCQ  I T I Y+ GL+ ++L      +GC LGCCLIP  ++D KDV H CPSC   +  ++R+
Sbjct:   88 VLQGEIFEGAPVQTVCPHCQQAITTKISYEIGLMNFVLGFFCCFMGCDLGCCLIPCLINDFKDVTHTCPSCKAYIYTYKRL 168
[Close]
BLAST of LITAF domain-containing protein vs. Ensembl Human
Match: CDIP1 (cell death inducing p53 target 1 [Source:HGNC Symbol;Acc:HGNC:13234])

HSP 1 Score: 48.9062 bits (115), Expect = 5.803e-7
Identity = 36/81 (44.44%), Postives = 52/81 (64.20%), Query Frame = 2 
Query:  209 IIQNQVYGCLPVNTVCPHCQAQIVTGIRYDNXXXXXXXXXXXXXXXXXXXXXXIPFCVDDCKDVIHVCPSCNMKVGEHRRV 451
            ++Q +++   PV TVCPHCQ  I T I Y+ GL+ ++L      +GC LGCCLIP  ++D KDV H CPSC   +  ++R+
Sbjct:   88 VLQGEIFEGAPVQTVCPHCQQAITTKISYEIGLMNFVLGFFCCFMGCDLGCCLIPCLINDFKDVTHTCPSCKAYIYTYKRL 168
[Close]
BLAST of LITAF domain-containing protein vs. Ensembl Human
Match: CDIP1 (cell death inducing p53 target 1 [Source:HGNC Symbol;Acc:HGNC:13234])

HSP 1 Score: 48.9062 bits (115), Expect = 8.570e-7
Identity = 36/81 (44.44%), Postives = 52/81 (64.20%), Query Frame = 2 
Query:  209 IIQNQVYGCLPVNTVCPHCQAQIVTGIRYDNXXXXXXXXXXXXXXXXXXXXXXIPFCVDDCKDVIHVCPSCNMKVGEHRRV 451
            ++Q +++   PV TVCPHCQ  I T I Y+ GL+ ++L      +GC LGCCLIP  ++D KDV H CPSC   +  ++R+
Sbjct:  127 VLQGEIFEGAPVQTVCPHCQQAITTKISYEIGLMNFVLGFFCCFMGCDLGCCLIPCLINDFKDVTHTCPSCKAYIYTYKRL 207
[Close]
BLAST of LITAF domain-containing protein vs. Ensembl Zebrafish
Match: cdip1 (cell death-inducing p53 target 1 [Source:ZFIN;Acc:ZDB-GENE-070720-18])

HSP 1 Score: 47.3654 bits (111), Expect = 2.357e-6
Identity = 37/81 (45.68%), Postives = 53/81 (65.43%), Query Frame = 2 
Query:  209 IIQNQVYGCLPVNTVCPHCQAQIVTGIRYDNXXXXXXXXXXXXXXXXXXXXXXIPFCVDDCKDVIHVCPSCNMKVGEHRRV 451
            ++Q +++   PV TVCPHCQ  I+T I +D GL+  L+C     +GC LGCCLIP  +DD KDV H CP+C   +  ++R+
Sbjct:  132 VLQGEMFQSAPVQTVCPHCQQPIITRISHDIGLMNTLVCMFCFFVGCDLGCCLIPCLIDDLKDVTHTCPNCKGYIYTYKRI 212
[Close]
BLAST of LITAF domain-containing protein vs. Ensembl Zebrafish
Match: cdip1 (cell death-inducing p53 target 1 [Source:ZFIN;Acc:ZDB-GENE-070720-18])

HSP 1 Score: 47.3654 bits (111), Expect = 2.357e-6
Identity = 37/81 (45.68%), Postives = 53/81 (65.43%), Query Frame = 2 
Query:  209 IIQNQVYGCLPVNTVCPHCQAQIVTGIRYDNXXXXXXXXXXXXXXXXXXXXXXIPFCVDDCKDVIHVCPSCNMKVGEHRRV 451
            ++Q +++   PV TVCPHCQ  I+T I +D GL+  L+C     +GC LGCCLIP  +DD KDV H CP+C   +  ++R+
Sbjct:  132 VLQGEMFQSAPVQTVCPHCQQPIITRISHDIGLMNTLVCMFCFFVGCDLGCCLIPCLIDDLKDVTHTCPNCKGYIYTYKRI 212
[Close]
BLAST of LITAF domain-containing protein vs. Ensembl Zebrafish
Match: litaf (lipopolysaccharide-induced TNF factor [Source:NCBI gene;Acc:431731])

HSP 1 Score: 46.2098 bits (108), Expect = 3.133e-6
Identity = 38/75 (50.67%), Postives = 51/75 (68.00%), Query Frame = 2 
Query:  224 VYGCLPVNTVCPHCQAQIVTGIRYDNXXXXXXXXXXXXXXXXXXXXXXIPFCVDDCKDVIHVCPSCNMKVGEHRR 448
            V+G +PV   CP C   ++T + Y +G L WL C GLA+ GC  GCCLIPFC++D KDV H CP+C+  +G H+R
Sbjct:   88 VFGNVPVQAHCPVCSQSVITRLEYSSGPLVWLSCAGLAVFGCIYGCCLIPFCIEDLKDVTHHCPNCSSVLGVHKR 162
[Close]
BLAST of LITAF domain-containing protein vs. Ensembl Xenopus
Match: ENSXETT00000031517.1 (LITAF domain containing [Source:NCBI gene;Acc:100498168])

HSP 1 Score: 49.6766 bits (117), Expect = 1.823e-7
Identity = 42/71 (59.15%), Postives = 53/71 (74.65%), Query Frame = 2 
Query:  239 PVNTVCPHCQAQIVTGIRYDNXXXXXXXXXXXXXXXXXXXXXXIPFCVDDCKDVIHVCPSCNMKVGEHRRV 451
            P +T CP C+  I+T I Y+ GLLTWLL G L + GCWLGCCLIPFCVD CKDV H CP+CN  + +++R+
Sbjct:   71 PASTTCPSCRQNIITNIHYNIGLLTWLLFGILFIFGCWLGCCLIPFCVDSCKDVDHYCPNCNHHLYKYKRL 141
[Close]
BLAST of LITAF domain-containing protein vs. Ensembl Mouse
Match: Cdip1 (cell death inducing Trp53 target 1 [Source:MGI Symbol;Acc:MGI:1913876])

HSP 1 Score: 48.521 bits (114), Expect = 9.281e-7
Identity = 36/81 (44.44%), Postives = 52/81 (64.20%), Query Frame = 2 
Query:  209 IIQNQVYGCLPVNTVCPHCQAQIVTGIRYDNXXXXXXXXXXXXXXXXXXXXXXIPFCVDDCKDVIHVCPSCNMKVGEHRRV 451
            ++Q +++   PV TVCPHCQ  I T I Y+ GL+ ++L      +GC LGCCLIP  ++D KDV H CPSC   +  ++R+
Sbjct:  110 VLQGEIFEGAPVQTVCPHCQQAITTKISYEIGLMNFVLGFFCCFMGCDLGCCLIPCLINDFKDVTHTCPSCKAYICTYKRL 190
[Close]
BLAST of LITAF domain-containing protein vs. Ensembl Mouse
Match: Cdip1 (cell death inducing Trp53 target 1 [Source:MGI Symbol;Acc:MGI:1913876])

HSP 1 Score: 48.521 bits (114), Expect = 1.024e-6
Identity = 36/81 (44.44%), Postives = 52/81 (64.20%), Query Frame = 2 
Query:  209 IIQNQVYGCLPVNTVCPHCQAQIVTGIRYDNXXXXXXXXXXXXXXXXXXXXXXIPFCVDDCKDVIHVCPSCNMKVGEHRRV 451
            ++Q +++   PV TVCPHCQ  I T I Y+ GL+ ++L      +GC LGCCLIP  ++D KDV H CPSC   +  ++R+
Sbjct:  127 VLQGEIFEGAPVQTVCPHCQQAITTKISYEIGLMNFVLGFFCCFMGCDLGCCLIPCLINDFKDVTHTCPSCKAYICTYKRL 207
[Close]
BLAST of LITAF domain-containing protein vs. Ensembl Mouse
Match: Cdip1 (cell death inducing Trp53 target 1 [Source:MGI Symbol;Acc:MGI:1913876])

HSP 1 Score: 48.521 bits (114), Expect = 1.024e-6
Identity = 36/81 (44.44%), Postives = 52/81 (64.20%), Query Frame = 2 
Query:  209 IIQNQVYGCLPVNTVCPHCQAQIVTGIRYDNXXXXXXXXXXXXXXXXXXXXXXIPFCVDDCKDVIHVCPSCNMKVGEHRRV 451
            ++Q +++   PV TVCPHCQ  I T I Y+ GL+ ++L      +GC LGCCLIP  ++D KDV H CPSC   +  ++R+
Sbjct:  127 VLQGEIFEGAPVQTVCPHCQQAITTKISYEIGLMNFVLGFFCCFMGCDLGCCLIPCLINDFKDVTHTCPSCKAYICTYKRL 207
[Close]
BLAST of LITAF domain-containing protein vs. UniProt/SwissProt
Match: sp|Q9H305|CDIP1_HUMAN (Cell death-inducing p53-target protein 1 OS=Homo sapiens OX=9606 GN=CDIP1 PE=1 SV=1)

HSP 1 Score: 48.9062 bits (115), Expect = 4.116e-6
Identity = 36/81 (44.44%), Postives = 52/81 (64.20%), Query Frame = 2 
Query:  209 IIQNQVYGCLPVNTVCPHCQAQIVTGIRYDNXXXXXXXXXXXXXXXXXXXXXXIPFCVDDCKDVIHVCPSCNMKVGEHRRV 451
            ++Q +++   PV TVCPHCQ  I T I Y+ GL+ ++L      +GC LGCCLIP  ++D KDV H CPSC   +  ++R+
Sbjct:  127 VLQGEIFEGAPVQTVCPHCQQAITTKISYEIGLMNFVLGFFCCFMGCDLGCCLIPCLINDFKDVTHTCPSCKAYIYTYKRL 207
[Close]
BLAST of LITAF domain-containing protein vs. UniProt/SwissProt
Match: sp|Q9DB75|CDIP1_MOUSE (Cell death-inducing p53-target protein 1 OS=Mus musculus OX=10090 GN=Cdip1 PE=2 SV=1)

HSP 1 Score: 48.521 bits (114), Expect = 7.161e-6
Identity = 36/81 (44.44%), Postives = 52/81 (64.20%), Query Frame = 2 
Query:  209 IIQNQVYGCLPVNTVCPHCQAQIVTGIRYDNXXXXXXXXXXXXXXXXXXXXXXIPFCVDDCKDVIHVCPSCNMKVGEHRRV 451
            ++Q +++   PV TVCPHCQ  I T I Y+ GL+ ++L      +GC LGCCLIP  ++D KDV H CPSC   +  ++R+
Sbjct:  127 VLQGEIFEGAPVQTVCPHCQQAITTKISYEIGLMNFVLGFFCCFMGCDLGCCLIPCLINDFKDVTHTCPSCKAYICTYKRL 207
[Close]
BLAST of LITAF domain-containing protein vs. UniProt/SwissProt
Match: sp|Q58D45|CDIP1_BOVIN (Cell death-inducing p53-target protein 1 OS=Bos taurus OX=9913 GN=CDIP1 PE=2 SV=1)

HSP 1 Score: 48.1358 bits (113), Expect = 7.739e-6
Identity = 36/81 (44.44%), Postives = 52/81 (64.20%), Query Frame = 2 
Query:  209 IIQNQVYGCLPVNTVCPHCQAQIVTGIRYDNXXXXXXXXXXXXXXXXXXXXXXIPFCVDDCKDVIHVCPSCNMKVGEHRRV 451
            ++Q +++   PV TVCPHCQ  I T I Y+ GL+ ++L      +GC LGCCLIP  ++D KDV H CPSC   +  ++R+
Sbjct:  127 VLQGEIFEGAPVQTVCPHCQQAITTKISYEIGLMNFVLGFFCCFMGCDLGCCLIPCLINDFKDVTHTCPSCKAYIYTYKRL 207
[Close]
BLAST of LITAF domain-containing protein vs. UniProt/SwissProt
Match: sp|Q5U2U6|CDIP1_RAT (Cell death-inducing p53-target protein 1 OS=Rattus norvegicus OX=10116 GN=Cdip1 PE=2 SV=1)

HSP 1 Score: 48.1358 bits (113), Expect = 9.959e-6
Identity = 36/81 (44.44%), Postives = 52/81 (64.20%), Query Frame = 2 
Query:  209 IIQNQVYGCLPVNTVCPHCQAQIVTGIRYDNXXXXXXXXXXXXXXXXXXXXXXIPFCVDDCKDVIHVCPSCNMKVGEHRRV 451
            ++Q +++   PV TVCPHCQ  I T I Y+ GL+ ++L      +GC LGCCLIP  ++D KDV H CPSC   +  ++R+
Sbjct:  127 VLQGEIFEGAPVQTVCPHCQQAITTKISYEIGLMNFVLGFFCCFMGCDLGCCLIPCLINDFKDVTHTCPSCKAYICTYKRL 207
[Close]
BLAST of LITAF domain-containing protein vs. TrEMBL
Match: A0A2B4RVD9 (Lipopolysaccharide-induced tumor necrosis factor-alpha factor-like OS=Stylophora pistillata OX=50429 GN=LITAF PE=4 SV=1)

HSP 1 Score: 65.4698 bits (158), Expect = 5.761e-10
Identity = 49/81 (60.49%), Postives = 60/81 (74.07%), Query Frame = 2 
Query:  209 IIQNQVYGCLPVNTVCPHCQAQIVTGIRYDNXXXXXXXXXXXXXXXXXXXXXXIPFCVDDCKDVIHVCPSCNMKVGEHRRV 451
            II N  +G  PV+ VCPHCQ  I+T   Y +G LTWL  G L LLGCWLGCCLIPFC+D CKDVIH CP+C+ ++G +RR+
Sbjct:   67 IIVNMAFGESPVSMVCPHCQTHIITCTTYQDGTLTWLSAGALFLLGCWLGCCLIPFCLDGCKDVIHSCPNCHARLGSYRRM 147
[Close]
BLAST of LITAF domain-containing protein vs. TrEMBL
Match: A0A3M6UJR4 (LITAF domain-containing protein OS=Pocillopora damicornis OX=46731 GN=pdam_00000257 PE=4 SV=1)

HSP 1 Score: 64.3142 bits (155), Expect = 1.133e-9
Identity = 47/78 (60.26%), Postives = 59/78 (75.64%), Query Frame = 2 
Query:  218 NQVYGCLPVNTVCPHCQAQIVTGIRYDNXXXXXXXXXXXXXXXXXXXXXXIPFCVDDCKDVIHVCPSCNMKVGEHRRV 451
            N ++G  PV+ VCPHCQ  I+T   Y +G LTWL  G L LLGCWLGCCLIPFC+D CKDVIH CP+C+ ++G +RR+
Sbjct:   67 NMIFGESPVSMVCPHCQTHIITCTTYQDGTLTWLSAGALCLLGCWLGCCLIPFCLDGCKDVIHSCPNCHARLGSYRRM 144
[Close]
BLAST of LITAF domain-containing protein vs. TrEMBL
Match: A0A194ANP1 (LITAF domain-containing protein OS=Pinctada fucata OX=50426 PE=4 SV=1)

HSP 1 Score: 61.2326 bits (147), Expect = 1.114e-8
Identity = 44/80 (55.00%), Postives = 58/80 (72.50%), Query Frame = 2 
Query:  212 IQNQVYGCLPVNTVCPHCQAQIVTGIRYDNXXXXXXXXXXXXXXXXXXXXXXIPFCVDDCKDVIHVCPSCNMKVGEHRRV 451
            ++N ++  +PV T CP CQA I T   Y+ G +TWL+ G L L+G WLGCCLIPFC+D CKDVIH CP+CN  VG++ R+
Sbjct:   47 LRNMMFREVPVATQCPFCQATITTSTSYETGTITWLVAGILLLMGLWLGCCLIPFCLDGCKDVIHRCPNCNQVVGKYTRM 126
[Close]
BLAST of LITAF domain-containing protein vs. TrEMBL
Match: A0A4E0QUK4 (Lipopolysaccharide induced TNF alpha factor OS=Fasciola hepatica OX=6192 GN=D915_009150 PE=4 SV=1)

HSP 1 Score: 61.2326 bits (147), Expect = 1.534e-8
Identity = 38/76 (50.00%), Postives = 47/76 (61.84%), Query Frame = 2 
Query:  224 VYGCLPVNTVCPHCQAQIVTGIRYDNXXXXXXXXXXXXXXXXXXXXXXIPFCVDDCKDVIHVCPSCNMKVGEHRRV 451
             +G +P    C HC  Q+VT + Y  G LTWLLC  +      L C LIPFCVDDCKD +H CP CNM +G +RR+
Sbjct:   61 TFGPVPTAAYCAHCNQQVVTEVHYVTGALTWLLCAIIFFFTGPLFCFLIPFCVDDCKDAVHSCPRCNMTLGTYRRL 136
[Close]
BLAST of LITAF domain-containing protein vs. TrEMBL
Match: V3ZDL5 (LITAF domain-containing protein OS=Lottia gigantea OX=225164 GN=LOTGIDRAFT_183210 PE=4 SV=1)

HSP 1 Score: 58.9214 bits (141), Expect = 3.309e-8
Identity = 39/71 (54.93%), Postives = 49/71 (69.01%), Query Frame = 2 
Query:  239 PVNTVCPHCQAQIVTGIRYDNXXXXXXXXXXXXXXXXXXXXXXIPFCVDDCKDVIHVCPSCNMKVGEHRRV 451
            PV+T CPHCQA+IVT   Y+ G L W++C  L  +G   GCCLIPFCVD CKDVIH CP+C   +  + R+
Sbjct:   11 PVHTKCPHCQAEIVTATHYETGTLAWIICVVLCFVGLGFGCCLIPFCVDACKDVIHSCPNCRQTISRYSRM 81
[Close]
BLAST of LITAF domain-containing protein vs. Ensembl Cavefish
Match: cdip1 (cell death inducing p53 target 1 [Source:NCBI gene;Acc:103033134])

HSP 1 Score: 47.7506 bits (112), Expect = 1.322e-6
Identity = 37/81 (45.68%), Postives = 52/81 (64.20%), Query Frame = 2 
Query:  209 IIQNQVYGCLPVNTVCPHCQAQIVTGIRYDNXXXXXXXXXXXXXXXXXXXXXXIPFCVDDCKDVIHVCPSCNMKVGEHRRV 451
            ++Q +++   PV TVCPHCQ  I+T I +D GL+  L C     +GC LGCCLIP  +DD KDV H CP+C   +  ++R+
Sbjct:  131 VLQGEMFQSAPVQTVCPHCQQAIITRISHDVGLMNTLFCLFCFFVGCDLGCCLIPCLIDDLKDVTHTCPNCKGYIYTYKRI 211
[Close]
BLAST of LITAF domain-containing protein vs. Ensembl Cavefish
Match: litaf (lipopolysaccharide-induced TNF factor [Source:NCBI gene;Acc:431731])

HSP 1 Score: 45.4394 bits (106), Expect = 4.769e-6
Identity = 38/78 (48.72%), Postives = 50/78 (64.10%), Query Frame = 2 
Query:  218 NQVYGCLPVNTVCPHCQAQIVTGIRYDNXXXXXXXXXXXXXXXXXXXXXXIPFCVDDCKDVIHVCPSCNMKVGEHRRV 451
              V+G  PV   CP+C   +VT + Y +G +TWL C GL + GC  GCCLIPFCVD  KDV H CP+C+  +G  +R+
Sbjct:   75 GMVFGHTPVQVHCPNCAQTVVTRLDYSSGTMTWLTCAGLFIFGCIYGCCLIPFCVDSLKDVTHHCPNCSSVLGVFKRL 152
[Close]
BLAST of LITAF domain-containing protein vs. Ensembl Nematostella
Match: EDO37753 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SES1])

HSP 1 Score: 51.6026 bits (122), Expect = 2.103e-9
Identity = 42/67 (62.69%), Postives = 49/67 (73.13%), Query Frame = 2 
Query:  251 VCPHCQAQIVTGIRYDNXXXXXXXXXXXXXXXXXXXXXXIPFCVDDCKDVIHVCPSCNMKVGEHRRV 451
            VC  CQ  IVT   Y  G LTWLL GG+ LLG WLGCCLIPFC+D CKDVIH CP+C  ++G +RR+
Sbjct:    2 VCSFCQTHIVTATTYRYGALTWLLSGGICLLGGWLGCCLIPFCLDGCKDVIHTCPNCQAQLGVYRRL 68
[Close]
BLAST of LITAF domain-containing protein vs. Ensembl Nematostella
Match: EDO33373 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SSF9])

HSP 1 Score: 48.1358 bits (113), Expect = 8.620e-8
Identity = 41/71 (57.75%), Postives = 48/71 (67.61%), Query Frame = 2 
Query:  239 PVNTVCPHCQAQIVTGIRYDNXXXXXXXXXXXXXXXXXXXXXXIPFCVDDCKDVIHVCPSCNMKVGEHRRV 451
            PV+  CP C A IVT   Y  G LTWL C GL ++GC LGCC IPFCVD  KDV+H CP+C  +VG  RR+
Sbjct:   33 PVSMNCPTCHATIVTATDYVTGTLTWLACFGLCIIGCDLGCCFIPFCVDSMKDVVHTCPNCRSQVGVFRRM 103
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BLAST of LITAF domain-containing protein vs. Ensembl Nematostella
Match: EDO42963 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S062])

HSP 1 Score: 45.4394 bits (106), Expect = 8.362e-7
Identity = 30/75 (40.00%), Postives = 41/75 (54.67%), Query Frame = 2 
Query:  227 YGCLPVNTVCPHCQAQIVTGIRYDNXXXXXXXXXXXXXXXXXXXXXXIPFCVDDCKDVIHVCPSCNMKVGEHRRV 451
            +G  P + VCP+CQA IVT   Y     T      L +   +  CC +PF +D CKDV+H CP+C  +VG   R+
Sbjct:   33 FGESPTSMVCPYCQATIVTATSY--VPGTCAWLICLVIFCFFWPCCFLPFVLDGCKDVVHSCPNCQRQVGVFTRL 105
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BLAST of LITAF domain-containing protein vs. Ensembl Medaka
Match: cdip1 (cell death inducing p53 target 1 [Source:NCBI gene;Acc:101173632])

HSP 1 Score: 47.3654 bits (111), Expect = 1.726e-6
Identity = 38/81 (46.91%), Postives = 51/81 (62.96%), Query Frame = 2 
Query:  209 IIQNQVYGCLPVNTVCPHCQAQIVTGIRYDNXXXXXXXXXXXXXXXXXXXXXXIPFCVDDCKDVIHVCPSCNMKVGEHRRV 451
            ++Q +++   PV TVCPHCQ  IVT I +D GL+  L C     +GC LGCCLIP  +DD KDV H CP C   +  ++R+
Sbjct:  124 VLQGEMFQTSPVQTVCPHCQQAIVTRISHDVGLMNTLFCLFCFFVGCDLGCCLIPCLIDDLKDVTHTCPYCKGYIYTYKRI 204
[Close]
BLAST of LITAF domain-containing protein vs. Planmine SMEST
Match: SMESG000020853.1 (SMESG000020853.1)

HSP 1 Score: 129.028 bits (323), Expect = 2.077e-38
Identity = 127/128 (99.22%), Postives = 127/128 (99.22%), Query Frame = 2 
Query:   68 MNSDNQPKNYENTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXIIQNQVYGCLPVNTVCPHCQAQIVTGIRYDNXXXXXXXXXXXXXXXXXXXXXXIPFCVDDCKDVIHVCPSCNMKVGEHRRV 451
            MNSDNQPKNYENTAPPPYPQNAPYPQAGQYQ GQPVQTGQPQVVYQTIIQNQVYGCLPVNTVCPHCQAQIVTGIRYDNGLLTWLLCGGLALLGCWLGCCLIPFCVDDCKDVIHVCPSCNMKVGEHRRV
Sbjct:    1 MNSDNQPKNYENTAPPPYPQNAPYPQAGQYQAGQPVQTGQPQVVYQTIIQNQVYGCLPVNTVCPHCQAQIVTGIRYDNGLLTWLLCGGLALLGCWLGCCLIPFCVDDCKDVIHVCPSCNMKVGEHRRV 128
[Close]
BLAST of LITAF domain-containing protein vs. Planmine SMEST
Match: SMESG000079265.1 (SMESG000079265.1)

HSP 1 Score: 59.3066 bits (142), Expect = 4.846e-12
Identity = 41/72 (56.94%), Postives = 50/72 (69.44%), Query Frame = 2 
Query:  236 LPVNTVCPHCQAQIVTGIRYDNXXXXXXXXXXXXXXXXXXXXXXIPFCVDDCKDVIHVCPSCNMKVGEHRRV 451
            +PV+ VCPHC + IVT +RYDNG LT+LL      +GC  GCCLIPF V   KDV H CP CN  +GE+RR+
Sbjct:    1 MPVSMVCPHCNSTIVTTVRYDNGTLTFLLAFITCFIGCVCGCCLIPFFVKQFKDVKHYCPRCNALLGEYRRI 72
[Close]
BLAST of LITAF domain-containing protein vs. Planmine SMEST
Match: SMESG000079265.1 (SMESG000079265.1)

HSP 1 Score: 54.299 bits (129), Expect = 3.468e-10
Identity = 39/67 (58.21%), Postives = 46/67 (68.66%), Query Frame = 2 
Query:  251 VCPHCQAQIVTGIRYDNXXXXXXXXXXXXXXXXXXXXXXIPFCVDDCKDVIHVCPSCNMKVGEHRRV 451
            VCPHC + IVT +RYDNG LT+LL      +GC  GCCLIPF V   KDV H CP CN  +GE+RR+
Sbjct:    2 VCPHCNSTIVTTVRYDNGTLTFLLAFITCFIGCVCGCCLIPFFVKQFKDVKHYCPRCNALLGEYRRI 68
The following BLAST results are available for this feature:
BLAST of LITAF domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
CDIP12.271e-744.44cell death inducing p53 target 1 [Source:HGNC Symb... [more]
CDIP12.271e-744.44cell death inducing p53 target 1 [Source:HGNC Symb... [more]
CDIP15.803e-744.44cell death inducing p53 target 1 [Source:HGNC Symb... [more]
CDIP15.803e-744.44cell death inducing p53 target 1 [Source:HGNC Symb... [more]
CDIP18.570e-744.44cell death inducing p53 target 1 [Source:HGNC Symb... [more]
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BLAST of LITAF domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of LITAF domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of LITAF domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 3
Match NameE-valueIdentityDescription
cdip12.357e-645.68cell death-inducing p53 target 1 [Source:ZFIN;Acc:... [more]
cdip12.357e-645.68cell death-inducing p53 target 1 [Source:ZFIN;Acc:... [more]
litaf3.133e-650.67lipopolysaccharide-induced TNF factor [Source:NCBI... [more]
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BLAST of LITAF domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ENSXETT00000031517.11.823e-759.15LITAF domain containing [Source:NCBI gene;Acc:1004... [more]
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BLAST of LITAF domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 3
Match NameE-valueIdentityDescription
Cdip19.281e-744.44cell death inducing Trp53 target 1 [Source:MGI Sym... [more]
Cdip11.024e-644.44cell death inducing Trp53 target 1 [Source:MGI Sym... [more]
Cdip11.024e-644.44cell death inducing Trp53 target 1 [Source:MGI Sym... [more]
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BLAST of LITAF domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 4
Match NameE-valueIdentityDescription
sp|Q9H305|CDIP1_HUMAN4.116e-644.44Cell death-inducing p53-target protein 1 OS=Homo s... [more]
sp|Q9DB75|CDIP1_MOUSE7.161e-644.44Cell death-inducing p53-target protein 1 OS=Mus mu... [more]
sp|Q58D45|CDIP1_BOVIN7.739e-644.44Cell death-inducing p53-target protein 1 OS=Bos ta... [more]
sp|Q5U2U6|CDIP1_RAT9.959e-644.44Cell death-inducing p53-target protein 1 OS=Rattus... [more]
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BLAST of LITAF domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A2B4RVD95.761e-1060.49Lipopolysaccharide-induced tumor necrosis factor-a... [more]
A0A3M6UJR41.133e-960.26LITAF domain-containing protein OS=Pocillopora dam... [more]
A0A194ANP11.114e-855.00LITAF domain-containing protein OS=Pinctada fucata... [more]
A0A4E0QUK41.534e-850.00Lipopolysaccharide induced TNF alpha factor OS=Fas... [more]
V3ZDL53.309e-854.93LITAF domain-containing protein OS=Lottia gigantea... [more]
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BLAST of LITAF domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
cdip11.322e-645.68cell death inducing p53 target 1 [Source:NCBI gene... [more]
litaf4.769e-648.72lipopolysaccharide-induced TNF factor [Source:NCBI... [more]
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BLAST of LITAF domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of LITAF domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of LITAF domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 3
Match NameE-valueIdentityDescription
EDO377532.103e-962.69Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO333738.620e-857.75Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO429638.362e-740.00Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of LITAF domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
cdip11.726e-646.91cell death inducing p53 target 1 [Source:NCBI gene... [more]
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BLAST of LITAF domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 3
Match NameE-valueIdentityDescription
SMESG000020853.12.077e-3899.22SMESG000020853.1[more]
SMESG000079265.14.846e-1256.94SMESG000079265.1[more]
SMESG000079265.13.468e-1058.21SMESG000079265.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30023825 ID=SMED30023825|Name=LITAF domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=600bp
TAATAAAAACTGTGTTAATATTTGAAATAATCTCATTAAAGAAATTTGAA
AACCGTATTAAATCGATATGAATTCAGATAATCAACCCAAAAATTATGAA
AACACTGCTCCTCCGCCATATCCTCAAAATGCCCCATATCCACAAGCCGG
GCAATACCAAACTGGACAACCTGTTCAAACAGGCCAACCTCAAGTTGTCT
ACCAAACAATCATACAAAACCAAGTTTACGGGTGCCTGCCGGTCAATACC
GTGTGCCCCCATTGCCAGGCCCAGATTGTCACAGGAATCAGATACGATAA
TGGATTGCTAACTTGGTTGCTGTGCGGTGGGCTCGCCTTGTTGGGCTGTT
GGTTAGGATGCTGTTTGATTCCGTTTTGCGTTGACGACTGCAAAGATGTG
ATTCACGTTTGTCCAAGTTGCAACATGAAAGTCGGAGAACACCGAAGAGT
TTAAATTGAGACCTAAATTTGAATTGATTAAAACAATCTAATTGATAATT
TCTTTAACAAAACTACTTATATACTGAATCAAACTCATTATCGTGTTTAT
TTGTTATTCGCTGAAATTGTTTTAATGGAAATTTACTAGGCAAAAAAAAA
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protein sequence of SMED30023825-orf-1

>SMED30023825-orf-1 ID=SMED30023825-orf-1|Name=SMED30023825-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=129bp
MNSDNQPKNYENTAPPPYPQNAPYPQAGQYQTGQPVQTGQPQVVYQTIIQ
NQVYGCLPVNTVCPHCQAQIVTGIRYDNGLLTWLLCGGLALLGCWLGCCL
IPFCVDDCKDVIHVCPSCNMKVGEHRRV*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000020protonephridia
PLANA:0000026gut
PLANA:0000034epidermis
PLANA:0000099neuron
PLANA:0000101muscle cell
PLANA:0002032epidermal cell
PLANA:0003116parenchymal cell
PLANA:0003117parapharyngeal cell
Vocabulary: INTERPRO
TermDefinition
IPR037519LITAF_fam
IPR006629LITAF
Vocabulary: cellular component
TermDefinition
GO:0016020membrane
GO:0016021integral component of membrane
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006629LPS-induced tumour necrosis factor alpha factorSMARTSM00714litafcoord: 58..127
e-value: 5.2E-20
score: 82.5
IPR006629LPS-induced tumour necrosis factor alpha factorPFAMPF10601zf-LITAF-likecoord: 58..125
e-value: 3.2E-24
score: 85.0
IPR006629LPS-induced tumour necrosis factor alpha factorPROSITEPS51837LITAFcoord: 43..127
score: 21.097
IPR037519LITAF domain containing proteinPANTHERPTHR23292LIPOPOLYSACCHARIDE-INDUCED TRANSCRIPTION FACTOR REGULATING TUMOR NECROSIS FACTOR ALPHAcoord: 4..127
NoneNo IPR availableTMHMMTMhelixcoord: 81..103