RNA cytidine acetyltransferase

Overview
NameRNA cytidine acetyltransferase
Smed IDSMED30023803
Length (bp)521
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of RNA cytidine acetyltransferase (SMED30023803) t-SNE clustered cells

Violin plots show distribution of expression levels for RNA cytidine acetyltransferase (SMED30023803) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of RNA cytidine acetyltransferase (SMED30023803) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for RNA cytidine acetyltransferase (SMED30023803) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30023803

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 4

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30023803h1SMcG0017052 SmedASXL_017225SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
non-ciliated neuronSMED30023803h1SMcG0017052 dd_Smed_v4_22465_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30023803h1SMcG0017052 Contig52842newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30023803h1SMcG0017052 Contig52842uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of RNA cytidine acetyltransferase vs. Ensembl Human
Match: NAT10 (N-acetyltransferase 10 [Source:HGNC Symbol;Acc:HGNC:29830])

HSP 1 Score: 51.2174 bits (121), Expect = 2.557e-7
Identity = 45/152 (29.61%), Postives = 73/152 (48.03%), Query Frame = 2 
Query:    5 PNYVNRGFGSRALTLLIEYATN---------LNSSHPIDGGSSMFNSLL--------------LPMSDLPKFPINYIGVLTELSVKLIKFFQRVDDFMVVFLESAINEITADCRGILIKRISN-DSVYSFNWLALFTKIFNKRY-ELMRYGY 385
            P+Y   G+GSRAL LL  Y            L +   I   SS   SLL              L +++ P   ++Y+GV   L+ +L+KF++R   F+ V+L    N++T +   I++K +++ D      WLA F K F +R+  L+ Y +
Sbjct:  633 PDYQGMGYGSRALQLLQMYYEGRFPCLEEKVLETPQEIHTVSSEAVSLLEEVITPRKDLPPLLLKLNERPAERLDYLGVSYGLTPRLLKFWKRA-GFVPVYLRQTPNDLTGEHSCIMLKTLTDEDEADQGGWLAAFWKDFRRRFLALLSYQF 783
[Close]
BLAST of RNA cytidine acetyltransferase vs. Ensembl Human
Match: NAT10 (N-acetyltransferase 10 [Source:HGNC Symbol;Acc:HGNC:29830])

HSP 1 Score: 50.8322 bits (120), Expect = 3.007e-7
Identity = 45/152 (29.61%), Postives = 73/152 (48.03%), Query Frame = 2 
Query:    5 PNYVNRGFGSRALTLLIEYATN---------LNSSHPIDGGSSMFNSLL--------------LPMSDLPKFPINYIGVLTELSVKLIKFFQRVDDFMVVFLESAINEITADCRGILIKRISN-DSVYSFNWLALFTKIFNKRY-ELMRYGY 385
            P+Y   G+GSRAL LL  Y            L +   I   SS   SLL              L +++ P   ++Y+GV   L+ +L+KF++R   F+ V+L    N++T +   I++K +++ D      WLA F K F +R+  L+ Y +
Sbjct:  561 PDYQGMGYGSRALQLLQMYYEGRFPCLEEKVLETPQEIHTVSSEAVSLLEEVITPRKDLPPLLLKLNERPAERLDYLGVSYGLTPRLLKFWKRA-GFVPVYLRQTPNDLTGEHSCIMLKTLTDEDEADQGGWLAAFWKDFRRRFLALLSYQF 711
[Close]
BLAST of RNA cytidine acetyltransferase vs. Ensembl Human
Match: NAT10 (N-acetyltransferase 10 [Source:HGNC Symbol;Acc:HGNC:29830])

HSP 1 Score: 50.8322 bits (120), Expect = 3.042e-7
Identity = 45/152 (29.61%), Postives = 73/152 (48.03%), Query Frame = 2 
Query:    5 PNYVNRGFGSRALTLLIEYATN---------LNSSHPIDGGSSMFNSLL--------------LPMSDLPKFPINYIGVLTELSVKLIKFFQRVDDFMVVFLESAINEITADCRGILIKRISN-DSVYSFNWLALFTKIFNKRY-ELMRYGY 385
            P+Y   G+GSRAL LL  Y            L +   I   SS   SLL              L +++ P   ++Y+GV   L+ +L+KF++R   F+ V+L    N++T +   I++K +++ D      WLA F K F +R+  L+ Y +
Sbjct:  480 PDYQGMGYGSRALQLLQMYYEGRFPCLEEKVLETPQEIHTVSSEAVSLLEEVITPRKDLPPLLLKLNERPAERLDYLGVSYGLTPRLLKFWKRA-GFVPVYLRQTPNDLTGEHSCIMLKTLTDEDEADQGGWLAAFWKDFRRRFLALLSYQF 630
[Close]
BLAST of RNA cytidine acetyltransferase vs. Ensembl Celegans
Match: nath-10 (RNA cytidine acetyltransferase [Source:UniProtKB/Swiss-Prot;Acc:O01757])

HSP 1 Score: 48.1358 bits (113), Expect = 1.031e-6
Identity = 39/152 (25.66%), Postives = 73/152 (48.03%), Query Frame = 2 
Query:    5 PNYVNRGFGSRALTLLIEY----ATNLNSSHPI-------------DGGS-SMFNSLLLPMSDLPKF----------PINYIGVLTELSVKLIKFFQRVDDFMVVFLESAINEITADCRGILIKRIS-----NDSVYSFNWLALFTKIFNKR 361
            P+Y + G+G RA+ L+ +Y    AT+L+                  DG +  +    + P +DLP             ++Y+GV   L+V L+KF++R ++F+ V++    N+IT +   I++K +      +D   S  WL ++ + F +R
Sbjct:  626 PDYQSMGYGGRAVQLIEQYYLGLATSLDEEEKAPAPPSKTVIKQVKDGHTVELLEERIEPRADLPPLLQRLDERKPERLDYLGVSFGLTVPLLKFWKR-NEFVPVYIRQNSNDITGEHTCIILKGLEHGGSDSDEEPSATWLPVYWREFRRR 776
[Close]
BLAST of RNA cytidine acetyltransferase vs. Ensembl Xenopus
Match: nat10 (N-acetyltransferase 10 (GCN5-related) [Source:NCBI gene;Acc:779537])

HSP 1 Score: 52.7582 bits (125), Expect = 6.755e-8
Identity = 45/152 (29.61%), Postives = 69/152 (45.39%), Query Frame = 2 
Query:    5 PNYVNRGFGSRALTLLIEY---------ATNLNSSHPIDGGSSMFNSLL--------------LPMSDLPKFPINYIGVLTELSVKLIKFFQRVDDFMVVFLESAINEITADCRGILIKRISNDSVYSFN-WLALFTKIFNKRY-ELMRYGY 385
            P+Y   G+GSRAL L+  Y             N    I   SS   SLL              L +S+ P   ++Y+GV   L+ KL+KF++R   F+ V+L    N++T +   +++K +  D       WL  F K F KR+  L+ Y +
Sbjct:  595 PDYQGMGYGSRALRLVQMYYEGQFPCLEENATNKPQEITSVSSEAVSLLEESLTPRKNLPPLLLRLSERPAEKLDYLGVSYGLTPKLLKFWKRA-GFVPVYLRQTPNDLTGEHSCVMLKTLHEDEESDQEPWLTAFWKDFRKRFLSLLSYQF 745
[Close]
BLAST of RNA cytidine acetyltransferase vs. Ensembl Xenopus
Match: nat10 (N-acetyltransferase 10 (GCN5-related) [Source:NCBI gene;Acc:779537])

HSP 1 Score: 52.7582 bits (125), Expect = 6.885e-8
Identity = 45/152 (29.61%), Postives = 69/152 (45.39%), Query Frame = 2 
Query:    5 PNYVNRGFGSRALTLLIEY---------ATNLNSSHPIDGGSSMFNSLL--------------LPMSDLPKFPINYIGVLTELSVKLIKFFQRVDDFMVVFLESAINEITADCRGILIKRISNDSVYSFN-WLALFTKIFNKRY-ELMRYGY 385
            P+Y   G+GSRAL L+  Y             N    I   SS   SLL              L +S+ P   ++Y+GV   L+ KL+KF++R   F+ V+L    N++T +   +++K +  D       WL  F K F KR+  L+ Y +
Sbjct:  633 PDYQGMGYGSRALRLVQMYYEGQFPCLEENATNKPQEITSVSSEAVSLLEESLTPRKNLPPLLLRLSERPAEKLDYLGVSYGLTPKLLKFWKRA-GFVPVYLRQTPNDLTGEHSCVMLKTLHEDEESDQEPWLTAFWKDFRKRFLSLLSYQF 783
[Close]
BLAST of RNA cytidine acetyltransferase vs. Ensembl Xenopus
Match: nat10 (N-acetyltransferase 10 (GCN5-related) [Source:NCBI gene;Acc:779537])

HSP 1 Score: 52.7582 bits (125), Expect = 6.886e-8
Identity = 45/152 (29.61%), Postives = 69/152 (45.39%), Query Frame = 2 
Query:    5 PNYVNRGFGSRALTLLIEY---------ATNLNSSHPIDGGSSMFNSLL--------------LPMSDLPKFPINYIGVLTELSVKLIKFFQRVDDFMVVFLESAINEITADCRGILIKRISNDSVYSFN-WLALFTKIFNKRY-ELMRYGY 385
            P+Y   G+GSRAL L+  Y             N    I   SS   SLL              L +S+ P   ++Y+GV   L+ KL+KF++R   F+ V+L    N++T +   +++K +  D       WL  F K F KR+  L+ Y +
Sbjct:  635 PDYQGMGYGSRALRLVQMYYEGQFPCLEENATNKPQEITSVSSEAVSLLEESLTPRKNLPPLLLRLSERPAEKLDYLGVSYGLTPKLLKFWKRA-GFVPVYLRQTPNDLTGEHSCVMLKTLHEDEESDQEPWLTAFWKDFRKRFLSLLSYQF 785
[Close]
BLAST of RNA cytidine acetyltransferase vs. Ensembl Xenopus
Match: nat10 (N-acetyltransferase 10 (GCN5-related) [Source:NCBI gene;Acc:779537])

HSP 1 Score: 52.373 bits (124), Expect = 6.944e-8
Identity = 45/152 (29.61%), Postives = 69/152 (45.39%), Query Frame = 2 
Query:    5 PNYVNRGFGSRALTLLIEY---------ATNLNSSHPIDGGSSMFNSLL--------------LPMSDLPKFPINYIGVLTELSVKLIKFFQRVDDFMVVFLESAINEITADCRGILIKRISNDSVYSFN-WLALFTKIFNKRY-ELMRYGY 385
            P+Y   G+GSRAL L+  Y             N    I   SS   SLL              L +S+ P   ++Y+GV   L+ KL+KF++R   F+ V+L    N++T +   +++K +  D       WL  F K F KR+  L+ Y +
Sbjct:  633 PDYQGMGYGSRALRLVQMYYEGQFPCLEENATNKPQEITSVSSEAVSLLEESLTPRKNLPPLLLRLSERPAEKLDYLGVSYGLTPKLLKFWKRA-GFVPVYLRQTPNDLTGEHSCVMLKTLHEDEESDQEPWLTAFWKDFRKRFLSLLSYQF 783
[Close]
BLAST of RNA cytidine acetyltransferase vs. Ensembl Xenopus
Match: nat10 (N-acetyltransferase 10 (GCN5-related) [Source:NCBI gene;Acc:779537])

HSP 1 Score: 52.373 bits (124), Expect = 6.988e-8
Identity = 45/152 (29.61%), Postives = 69/152 (45.39%), Query Frame = 2 
Query:    5 PNYVNRGFGSRALTLLIEY---------ATNLNSSHPIDGGSSMFNSLL--------------LPMSDLPKFPINYIGVLTELSVKLIKFFQRVDDFMVVFLESAINEITADCRGILIKRISNDSVYSFN-WLALFTKIFNKRY-ELMRYGY 385
            P+Y   G+GSRAL L+  Y             N    I   SS   SLL              L +S+ P   ++Y+GV   L+ KL+KF++R   F+ V+L    N++T +   +++K +  D       WL  F K F KR+  L+ Y +
Sbjct:  637 PDYQGMGYGSRALRLVQMYYEGQFPCLEENATNKPQEITSVSSEAVSLLEESLTPRKNLPPLLLRLSERPAEKLDYLGVSYGLTPKLLKFWKRA-GFVPVYLRQTPNDLTGEHSCVMLKTLHEDEESDQEPWLTAFWKDFRKRFLSLLSYQF 787
[Close]
BLAST of RNA cytidine acetyltransferase vs. Ensembl Mouse
Match: Nat10 (N-acetyltransferase 10 [Source:MGI Symbol;Acc:MGI:2138939])

HSP 1 Score: 50.8322 bits (120), Expect = 2.246e-7
Identity = 45/152 (29.61%), Postives = 73/152 (48.03%), Query Frame = 2 
Query:    5 PNYVNRGFGSRALTLLIEYATN---------LNSSHPIDGGSSMFNSLL--------------LPMSDLPKFPINYIGVLTELSVKLIKFFQRVDDFMVVFLESAINEITADCRGILIKRISN-DSVYSFNWLALFTKIFNKRY-ELMRYGY 385
            P+Y   G+GSRAL LL  Y            L +   I   SS   SLL              L +++ P   ++Y+GV   L+ +L+KF++R   F+ V+L    N++T +   I++K +++ D      WLA F K F +R+  L+ Y +
Sbjct:  633 PDYQGMGYGSRALQLLQMYYEGKFPCLEEKVLETPQEIRTVSSEAVSLLEEVITPRKDLPPLLLKLNERPAERLDYLGVSYGLTPRLLKFWKRA-GFVPVYLRQTPNDLTGEHSCIMLKTLADEDEAEQGAWLAAFWKDFRRRFLALLSYQF 783
[Close]
BLAST of RNA cytidine acetyltransferase vs. UniProt/SwissProt
Match: sp|Q9H0A0|NAT10_HUMAN (RNA cytidine acetyltransferase OS=Homo sapiens OX=9606 GN=NAT10 PE=1 SV=2)

HSP 1 Score: 51.2174 bits (121), Expect = 1.228e-6
Identity = 45/152 (29.61%), Postives = 73/152 (48.03%), Query Frame = 2 
Query:    5 PNYVNRGFGSRALTLLIEYATN---------LNSSHPIDGGSSMFNSLL--------------LPMSDLPKFPINYIGVLTELSVKLIKFFQRVDDFMVVFLESAINEITADCRGILIKRISN-DSVYSFNWLALFTKIFNKRY-ELMRYGY 385
            P+Y   G+GSRAL LL  Y            L +   I   SS   SLL              L +++ P   ++Y+GV   L+ +L+KF++R   F+ V+L    N++T +   I++K +++ D      WLA F K F +R+  L+ Y +
Sbjct:  633 PDYQGMGYGSRALQLLQMYYEGRFPCLEEKVLETPQEIHTVSSEAVSLLEEVITPRKDLPPLLLKLNERPAERLDYLGVSYGLTPRLLKFWKRA-GFVPVYLRQTPNDLTGEHSCIMLKTLTDEDEADQGGWLAAFWKDFRRRFLALLSYQF 783
[Close]
BLAST of RNA cytidine acetyltransferase vs. UniProt/SwissProt
Match: sp|Q8K224|NAT10_MOUSE (RNA cytidine acetyltransferase OS=Mus musculus OX=10090 GN=Nat10 PE=1 SV=1)

HSP 1 Score: 50.8322 bits (120), Expect = 1.571e-6
Identity = 45/152 (29.61%), Postives = 73/152 (48.03%), Query Frame = 2 
Query:    5 PNYVNRGFGSRALTLLIEYATN---------LNSSHPIDGGSSMFNSLL--------------LPMSDLPKFPINYIGVLTELSVKLIKFFQRVDDFMVVFLESAINEITADCRGILIKRISN-DSVYSFNWLALFTKIFNKRY-ELMRYGY 385
            P+Y   G+GSRAL LL  Y            L +   I   SS   SLL              L +++ P   ++Y+GV   L+ +L+KF++R   F+ V+L    N++T +   I++K +++ D      WLA F K F +R+  L+ Y +
Sbjct:  633 PDYQGMGYGSRALQLLQMYYEGKFPCLEEKVLETPQEIRTVSSEAVSLLEEVITPRKDLPPLLLKLNERPAERLDYLGVSYGLTPRLLKFWKRA-GFVPVYLRQTPNDLTGEHSCIMLKTLADEDEAEQGAWLAAFWKDFRRRFLALLSYQF 783
[Close]
BLAST of RNA cytidine acetyltransferase vs. TrEMBL
Match: E0VDN5 (RNA cytidine acetyltransferase OS=Pediculus humanus subsp. corporis OX=121224 GN=8238355 PE=3 SV=1)

HSP 1 Score: 65.4698 bits (158), Expect = 3.723e-9
Identity = 44/141 (31.21%), Postives = 71/141 (50.35%), Query Frame = 2 
Query:    5 PNYVNRGFGSRALTLLIEYATNLNS---SHPIDGG------------------SSMFNSLLLPMSDLPKFPINYIGVLTELSVKLIKFFQRVDDFMVVFLESAINEITADCRGILIKRISNDSVYSFNWLALFTKIFNKRY 364
            P+Y   G+GSRAL LL +Y +N  S     P++                     S    LL+ +S+ P   ++YIGV   L+ +L+KF+++ D FM V++    N++T +   IL+K I  D     +WL  F+K F +R+
Sbjct:  629 PDYQGMGYGSRALALLEDYYSNKISYLDDAPVEENIASVQDESLGLLEEQIVPRSSLPPLLMKLSERPAEQLDYIGVSFGLTQELLKFWKKAD-FMPVYIRQTKNDLTGENSCILLKSIKKDEDNEKDWLIHFSKDFKRRF 768
[Close]
BLAST of RNA cytidine acetyltransferase vs. TrEMBL
Match: A0A1I8CBM2 (RNA cytidine acetyltransferase OS=Rhabditophanes sp. KR3021 OX=114890 PE=3 SV=1)

HSP 1 Score: 63.5438 bits (153), Expect = 1.820e-8
Identity = 45/157 (28.66%), Postives = 81/157 (51.59%), Query Frame = 2 
Query:    5 PNYVNRGFGSRALTLLIEY--------ATNLNSSHPIDGGSSM------------FNSLLLPMSDLPKFPINYIGVLTELSVKLIKFFQRVDDFMVVFLESAINEITADCRGILIKRISNDSVYSFNWLALFTKIFNKRYELMRYGYLRGLDEDFKT 415
            P+Y ++G+G RA+ LL +Y        A    +   ++    +              SLLL +++ P   ++Y+GV   L+V L+KF++R  +F+ VF+  ++NE+T +   +++K ++ND   S +WL  + + F  RY L   GY      DFK+
Sbjct:  621 PDYQSKGYGKRAIQLLTDYYEGKIPITAQATKAIRQVETDEVLNVLEENISPRDNLPSLLLKLNERPAEKLDYLGVSYGLTVNLLKFWKR-SNFVPVFIRQSVNELTDEHTIVMLKSLNNDGAISPSWLINYWEEFKHRY-LSLLGY------DFKS 769
[Close]
BLAST of RNA cytidine acetyltransferase vs. TrEMBL
Match: A0A1Y3BKK0 (RNA cytidine acetyltransferase OS=Euroglyphus maynei OX=6958 GN=BLA29_000176 PE=3 SV=1)

HSP 1 Score: 59.6918 bits (143), Expect = 4.262e-7
Identity = 39/136 (28.68%), Postives = 71/136 (52.21%), Query Frame = 2 
Query:    2 DPNYVNRGFGSRALTLLIEYATNLNSSHPI-------------DGGSSMFNSLLLPMSDLPKFPINYIGVLTELSVKLIKFFQRVDDFMVVFLESAINEITADCRGILIKR--ISNDSVYSFNWLALFTKIFNKRY 364
            DPN+   G+G+RAL LL  Y   L++ + I             D    +   LLL + +     ++Y+GV   ++ +L++F+++   F+ V+L    NE+T +   I++K+  + NDS+ + NWL  F   F +R+
Sbjct:  616 DPNHQKMGYGTRALQLLEHYYRGLHNVNLISDERNESDCEQNHDAIKKLDEPLLLDLKERKAEKLDYLGVSFGMTQELLRFWKK-SGFIPVYLRQTPNELTGEHSCIMLKQLAVENDSIATNNWLQEFWIDFRRRF 750
[Close]
BLAST of RNA cytidine acetyltransferase vs. TrEMBL
Match: A0A084G500 (RNA cytidine acetyltransferase OS=Scedosporium apiospermum OX=563466 GN=NAT10 PE=3 SV=1)

HSP 1 Score: 59.3066 bits (142), Expect = 5.666e-7
Identity = 48/169 (28.40%), Postives = 78/169 (46.15%), Query Frame = 2 
Query:    2 DPNYVNRGFGSRALTLLIEYA----TNLNSSHPIDGGSSMF----------NSLL---LPMSDLPKFP-------------INYIGVLTELSVKLIKFFQRVDDFMVVFLESAINEITADCRGILIKRISNDSVYSFNWLALFTKIFNKRYELMRYGYLRGLDEDFKTL 418
            +P+YV+ G+GSRAL LL+ Y     TNL+   P+      F           SLL   + + D+ K P             ++Y+GV   L+  L KF+++   F  V+L    NE+T +   ++++ + +    S  WL  F + F++R       YL  L  DF+T 
Sbjct:  643 NPDYVSMGYGSRALDLLVNYYDRKFTNLSEEEPMTSAEETFPRVTDDELAGASLLDDNIKVRDISKMPPLFARLEERRPASVDYVGVSYGLTPPLHKFWKKA-TFAPVYLRQTPNELTGEHTCVMLRPLESSGDQS--WLGAFARDFHRR-------YLSLLSYDFRTF 801
[Close]
BLAST of RNA cytidine acetyltransferase vs. TrEMBL
Match: A0A0C3QM96 (RNA cytidine acetyltransferase OS=Tulasnella calospora MUT 4182 OX=1051891 GN=NAT10 PE=3 SV=1)

HSP 1 Score: 59.3066 bits (142), Expect = 5.671e-7
Identity = 42/155 (27.10%), Postives = 77/155 (49.68%), Query Frame = 2 
Query:    5 PNYVNRGFGSRALTLLI-----EYATNLNSSHP----------IDGGSSMFNSLLLP------------MSDLPKFPINYIGVLTELSVKLIKFFQRVDDFMVVFLESAINEITADCRGILIKRISNDSVYSFNWLALFTKIFNKRY-ELMRYGY 385
            P+Y N G+G+RAL  L      EY  NL+ S            ID  +++ N  + P            +S L   P++Y+GV   L+ +L++F++R   ++ +++    NE+T +C  +++K ++ DS      L  F K F +R+  L+ +G+
Sbjct:  649 PDYSNMGYGARALQALELFYRGEY-FNLDESEAVKGEYVRNEHIDQDATLDNETITPRAITSLPPLLQTLSSLKPPPLDYMGVSYGLTAQLLRFWKR-SKYVPLYVRQTKNELTGECSCVMLKSLAGDSTQPVQGLEEFVKDFRRRFLNLLGFGF 801
[Close]
BLAST of RNA cytidine acetyltransferase vs. Ensembl Cavefish
Match: nat10 (N-acetyltransferase 10 [Source:NCBI gene;Acc:103034875])

HSP 1 Score: 49.2914 bits (116), Expect = 5.390e-7
Identity = 39/154 (25.32%), Postives = 72/154 (46.75%), Query Frame = 2 
Query:    2 DPNYVNRGFGSRALTLLIEY---------------ATNLNSSHPIDGGSSMFNSLLLPMSDLPKF----------PINYIGVLTELSVKLIKFFQRVDDFMVVFLESAINEITADCRGILIKRISNDSVYSFNWLALFTKIFNKRY-ELMRYGY 385
            +P+Y   G+GSRAL LL  Y               A+ + S        S+   ++ P +DLP             ++Y+GV   L+ +L+KF+++   F+ V+L    N++T +   +++K ++ +      WL  F K F +R+  L+ Y +
Sbjct:  634 NPDYQGMGYGSRALQLLELYYKGQFPVLDERERPTASKITSVS--SEAVSLLEEVVSPRTDLPPLLLKLSERTAERLDYLGVSYGLTPQLLKFWKKA-GFVPVYLRQTPNDLTGEHSCVMLKELNTEDSEG-QWLPAFWKDFRRRFLSLLSYQF 783
[Close]
BLAST of RNA cytidine acetyltransferase vs. Ensembl Cavefish
Match: nat10 (N-acetyltransferase 10 [Source:NCBI gene;Acc:103034875])

HSP 1 Score: 48.9062 bits (115), Expect = 6.912e-7
Identity = 39/154 (25.32%), Postives = 72/154 (46.75%), Query Frame = 2 
Query:    2 DPNYVNRGFGSRALTLLIEY---------------ATNLNSSHPIDGGSSMFNSLLLPMSDLPKF----------PINYIGVLTELSVKLIKFFQRVDDFMVVFLESAINEITADCRGILIKRISNDSVYSFNWLALFTKIFNKRY-ELMRYGY 385
            +P+Y   G+GSRAL LL  Y               A+ + S        S+   ++ P +DLP             ++Y+GV   L+ +L+KF+++   F+ V+L    N++T +   +++K ++ +      WL  F K F +R+  L+ Y +
Sbjct:  634 NPDYQGMGYGSRALQLLELYYKGQFPVLDERERPTASKITSVS--SEAVSLLEEVVSPRTDLPPLLLKLSERTAERLDYLGVSYGLTPQLLKFWKKA-GFVPVYLRQTPNDLTGEHSCVMLKELNTEDSEG-QWLPAFWKDFRRRFLSLLSYQF 783
[Close]
BLAST of RNA cytidine acetyltransferase vs. Ensembl Sea Lamprey
Match: nat10 (N-acetyltransferase 10 [Source:ZFIN;Acc:ZDB-GENE-040426-1543])

HSP 1 Score: 52.373 bits (124), Expect = 8.381e-9
Identity = 43/153 (28.10%), Postives = 68/153 (44.44%), Query Frame = 2 
Query:    5 PNYVNRGFGSRALTLLIEY-------------ATNLNSSHPIDGGSSMFNSLLLPMSDLPKF----------PINYIGVLTELSVKLIKFFQRVDDFMVVFLESAINEITADCRGILIKRISNDSVYSF--NWLALFTKIFNKRY-ELMRYGY 385
            P+Y   G+GSRAL LL  Y              T  + S        +    L P  DLP             ++Y+GV   L+ +L+KF++R   F  V+L    N++T +   I++K ++ D       NWLA + K F +R+  L+ Y +
Sbjct:  636 PDYQGMGYGSRALRLLQMYYEGKFPCLDETASQTQGHISSIASDAVGLLEETLTPRRDLPPLLHKLSERPTERLDYLGVSFGLTPQLLKFWKR-SGFTPVYLRQTPNDLTGEHSCIMLKVLNEDEQMERPENWLAAYWKDFRRRFVTLLSYQF 787
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BLAST of RNA cytidine acetyltransferase vs. Planmine SMEST
Match: SMESG000066293.1 (SMESG000066293.1)

HSP 1 Score: 285.419 bits (729), Expect = 1.971e-92
Identity = 156/159 (98.11%), Postives = 158/159 (99.37%), Query Frame = 2 
Query:    2 DPNYVNRGFGSRALTLLIEYATNLNSSHPIDGGSSMFNSLLLPMSDLPKFPINYIGVLTELSVKLIKFFQRVDDFMVVFLESAINEITADCRGILIKRISNDSVYSFNWLALFTKIFNKRYELMRYGYLRGLDEDFKTLLXXXXXXXXXXXXXXXLPDE 478
            DPNYVNRGFGSRALTLLIEYATNL+SSHPI+G SSMFNSLLLPMSDLPKFPINYIGVLTELSVKLIKFFQRVDDFMVVFLESAINEITADCRGILIKRISNDSVYSFNWLALFTKIFNKRYELMRYGYLRGLDEDFKTLLSNSSFSKFFEREFKNLPDE
Sbjct:  625 DPNYVNRGFGSRALTLLIEYATNLHSSHPINGDSSMFNSLLLPMSDLPKFPINYIGVLTELSVKLIKFFQRVDDFMVVFLESAINEITADCRGILIKRISNDSVYSFNWLALFTKIFNKRYELMRYGYLRGLDEDFKTLLSNSSFSKFFEREFKNLPDE 783
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BLAST of RNA cytidine acetyltransferase vs. Planmine SMEST
Match: SMESG000026207.1 (SMESG000026207.1)

HSP 1 Score: 56.6102 bits (135), Expect = 1.311e-9
Identity = 41/159 (25.79%), Postives = 83/159 (52.20%), Query Frame = 2 
Query:    5 PNYVNRGFGSRALTLLIEYATNLNSS----------HPIDGGSSMFNS---------------LLLPMSDLPKFPINYIGVLTELSVKLIKFFQRVDDFMVVFLESAINEITADCRGILIKRIS-NDSVY-SFNWLALFTKIFNKRYELMRYGYLRGLD 400
            PN+  +G+G +AL LL EY     +S          H I   +S +N+               LL+ +SD+P   ++YI V   L+ +L++F+++ ++F+ ++L   IN+ T +   ++++++S ++SV  +F WL  +   F +R+  +  G  + +D
Sbjct:  630 PNFQRQGYGGQALKLLYEYFNQGKTSSAAKVQKTTLHDIVKSNSSYNNFSTVNNIPPVDKLGHLLVKLSDIPAESLDYISVSFGLTSELLRFWKK-NNFIPIYLRQTINDATGEHSCVMLRQLSKSESVEGNFQWLQAYNNDFIRRFCNLLPGPFKKID 787
The following BLAST results are available for this feature:
BLAST of RNA cytidine acetyltransferase vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 3
Match NameE-valueIdentityDescription
NAT102.557e-729.61N-acetyltransferase 10 [Source:HGNC Symbol;Acc:HGN... [more]
NAT103.007e-729.61N-acetyltransferase 10 [Source:HGNC Symbol;Acc:HGN... [more]
NAT103.042e-729.61N-acetyltransferase 10 [Source:HGNC Symbol;Acc:HGN... [more]
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BLAST of RNA cytidine acetyltransferase vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 1
Match NameE-valueIdentityDescription
nath-101.031e-625.66RNA cytidine acetyltransferase [Source:UniProtKB/... [more]
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BLAST of RNA cytidine acetyltransferase vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of RNA cytidine acetyltransferase vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of RNA cytidine acetyltransferase vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
nat106.755e-829.61N-acetyltransferase 10 (GCN5-related) [Source:NCBI... [more]
nat106.885e-829.61N-acetyltransferase 10 (GCN5-related) [Source:NCBI... [more]
nat106.886e-829.61N-acetyltransferase 10 (GCN5-related) [Source:NCBI... [more]
nat106.944e-829.61N-acetyltransferase 10 (GCN5-related) [Source:NCBI... [more]
nat106.988e-829.61N-acetyltransferase 10 (GCN5-related) [Source:NCBI... [more]
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BLAST of RNA cytidine acetyltransferase vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Nat102.246e-729.61N-acetyltransferase 10 [Source:MGI Symbol;Acc:MGI:... [more]
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BLAST of RNA cytidine acetyltransferase vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 2
Match NameE-valueIdentityDescription
sp|Q9H0A0|NAT10_HUMAN1.228e-629.61RNA cytidine acetyltransferase OS=Homo sapiens OX=... [more]
sp|Q8K224|NAT10_MOUSE1.571e-629.61RNA cytidine acetyltransferase OS=Mus musculus OX=... [more]
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BLAST of RNA cytidine acetyltransferase vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
E0VDN53.723e-931.21RNA cytidine acetyltransferase OS=Pediculus humanu... [more]
A0A1I8CBM21.820e-828.66RNA cytidine acetyltransferase OS=Rhabditophanes s... [more]
A0A1Y3BKK04.262e-728.68RNA cytidine acetyltransferase OS=Euroglyphus mayn... [more]
A0A084G5005.666e-728.40RNA cytidine acetyltransferase OS=Scedosporium api... [more]
A0A0C3QM965.671e-727.10RNA cytidine acetyltransferase OS=Tulasnella calos... [more]
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BLAST of RNA cytidine acetyltransferase vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
nat105.390e-725.32N-acetyltransferase 10 [Source:NCBI gene;Acc:10303... [more]
nat106.912e-725.32N-acetyltransferase 10 [Source:NCBI gene;Acc:10303... [more]
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BLAST of RNA cytidine acetyltransferase vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
nat108.381e-928.10N-acetyltransferase 10 [Source:ZFIN;Acc:ZDB-GENE-0... [more]
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BLAST of RNA cytidine acetyltransferase vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of RNA cytidine acetyltransferase vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of RNA cytidine acetyltransferase vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of RNA cytidine acetyltransferase vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000066293.11.971e-9298.11SMESG000066293.1[more]
SMESG000026207.11.311e-925.79SMESG000026207.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30023803 ID=SMED30023803|Name=RNA cytidine acetyltransferase|organism=Schmidtea mediterranea sexual|type=transcript|length=521bp
CGATCCGAATTATGTTAATCGAGGATTTGGAAGCAGAGCTCTGACTCTGT
TGATTGAATACGCTACGAATTTGAATTCCAGTCACCCAATTGATGGGGGT
TCGTCGATGTTCAATTCACTTTTACTGCCGATGTCGGACTTGCCTAAGTT
TCCAATCAATTACATTGGAGTTTTAACGGAGCTCAGTGTGAAACTGATCA
AATTTTTTCAGCGTGTTGATGATTTTATGGTAGTATTTCTGGAATCGGCA
ATAAATGAAATCACAGCCGATTGCAGAGGAATCCTCATTAAAAGAATATC
CAATGACTCCGTGTATTCCTTTAATTGGCTCGCTTTATTCACTAAAATAT
TTAATAAACGCTATGAACTCATGCGATATGGATACTTGAGGGGTCTTGAT
GAAGATTTCAAAACGTTGTTGTCAAATTCTTCATTTTCTAAATTCTTTGA
AAGGGAATTCAAAAATTTACCAGATGAAAATATAAGATCTTTTAGCATTT
GAATAAATATTTAAGATTTTA
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protein sequence of SMED30023803-orf-1

>SMED30023803-orf-1 ID=SMED30023803-orf-1|Name=SMED30023803-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=167bp
DPNYVNRGFGSRALTLLIEYATNLNSSHPIDGGSSMFNSLLLPMSDLPKF
PINYIGVLTELSVKLIKFFQRVDDFMVVFLESAINEITADCRGILIKRIS
NDSVYSFNWLALFTKIFNKRYELMRYGYLRGLDEDFKTLLSNSSFSKFFE
REFKNLPDENIRSFSI*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: molecular function
TermDefinition
GO:0000049tRNA binding
GO:0030515snoRNA binding
GO:1990883rRNA cytidine N-acetyltransferase activity
GO:0000166nucleotide binding
GO:0005524ATP binding
GO:0008080N-acetyltransferase activity
GO:0016740transferase activity
GO:0016746transferase activity, transferring acyl groups
Vocabulary: biological process
TermDefinition
GO:0016556mRNA modification
GO:0000154rRNA modification
GO:0006364rRNA processing
GO:0008033tRNA processing
GO:0016072rRNA metabolic process
GO:0034470ncRNA processing
GO:0042274ribosomal small subunit biogenesis
GO:0051391tRNA acetylation
Vocabulary: cellular component
TermDefinition
GO:0030688preribosome, small subunit precursor
GO:0005634nucleus
GO:0005730nucleolus
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0002109X1 cell