F-box domain-containing protein

Overview


Name	F-box domain-containing protein
Smed ID	SMED30023758
Length (bp)	812

Neoblast Clusters

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.

Expression of F-box domain-containing protein (SMED30023758) t-SNE clustered cells

Violin plots show distribution of expression levels for F-box domain-containing protein (SMED30023758) in cells (dots) of each of the 12 neoblast clusters.

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of F-box domain-containing protein (SMED30023758) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for F-box domain-containing protein (SMED30023758) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30023758

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 10

Expressed In	Reference Transcript	Gene Models	Published Transcript	Transcriptome	Publication
oocyte	SMED30023758	h1SMcG0022988	Contig27999	newmark_ests	PMID:28434803 Rouhana et al., 2017
oocyte	SMED30023758	h1SMcG0022988	Contig27999	uc_Smed_v2	PMID:28434803 Rouhana et al., 2017
vitelline gland	SMED30023758	h1SMcG0022988	Contig27999	newmark_ests	PMID:28434803 Rouhana et al., 2017
vitelline gland	SMED30023758	h1SMcG0022988	Contig27999	uc_Smed_v2	PMID:28434803 Rouhana et al., 2017
oocyte	SMED30023758	h1SMcG0022988	Contig27999	newmark_ests	PMID:28434803 Rouhana et al., 2017
oocyte	SMED30023758	h1SMcG0022988	Contig27999	uc_Smed_v2	PMID:28434803 Rouhana et al., 2017
vitelline gland	SMED30023758	h1SMcG0022988	Contig27999	newmark_ests	PMID:28434803 Rouhana et al., 2017
vitelline gland	SMED30023758	h1SMcG0022988	Contig27999	uc_Smed_v2	PMID:28434803 Rouhana et al., 2017
nervous system	SMED30023758	h1SMcG0002141	dd_Smed_v6_8211_0_1	dd_Smed_v6	PMID:29291974 Strand et al., 2018
nervous system	SMED30023758	h1SMcG0002141	dd_Smed_v6_8211_0_1	dd_Smed_v6	PMID:29291974 Strand et al., 2018

Note: Hover over icons to view figure legend

Homology

[Close]

BLAST of F-box domain-containing protein vs. Ensembl Human
Match: FBXW7 (F-box and WD repeat domain containing 7 [Source:HGNC Symbol;Acc:HGNC:16712])

HSP 1 Score: 48.1358 bits (113), Expect = 9.247e-6
Identity = 19/50 (38.00%), Postives = 31/50 (62.00%), Query Frame = 1

Query:   70 RNYIDHLPTELFFHIFKFINIKDLLSCSQVSKSWHSLSNDDLVWTSKCRQ 219
            R++I  LP EL  ++  F+  KDLL  +Q  + W  L+ D+L+W  KC++
Sbjct:  198 RDFISLLPKELALYVLSFLEPKDLLQAAQTCRYWRILAEDNLLWREKCKE 247

[Close]

BLAST of F-box domain-containing protein vs. Ensembl Fly
Match: ago (gene:FBgn0041171 transcript:FBtr0073246)

HSP 1 Score: 52.373 bits (124), Expect = 2.130e-7
Identity = 21/54 (38.89%), Postives = 32/54 (59.26%), Query Frame = 1

Query:   70 RNYIDHLPTELFFHIFKFINIKDLLSCSQVSKSWHSLSNDDLVWTSKCRQITFL 231
            R++I  LP EL   +  ++  KDLL  +Q  +SW  L +D+L+W  KCR+   L
Sbjct:  889 RDFISLLPRELALFVLSYLEPKDLLRAAQTCRSWRFLCDDNLLWKEKCRKAQIL 942

[Close]

BLAST of F-box domain-containing protein vs. Ensembl Fly
Match: ago (gene:FBgn0041171 transcript:FBtr0073245)

HSP 1 Score: 52.373 bits (124), Expect = 2.130e-7
Identity = 21/54 (38.89%), Postives = 32/54 (59.26%), Query Frame = 1

Query:   70 RNYIDHLPTELFFHIFKFINIKDLLSCSQVSKSWHSLSNDDLVWTSKCRQITFL 231
            R++I  LP EL   +  ++  KDLL  +Q  +SW  L +D+L+W  KCR+   L
Sbjct:  889 RDFISLLPRELALFVLSYLEPKDLLRAAQTCRSWRFLCDDNLLWKEKCRKAQIL 942

[Close]

BLAST of F-box domain-containing protein vs. Ensembl Fly
Match: ago (gene:FBgn0041171 transcript:FBtr0073247)

HSP 1 Score: 52.373 bits (124), Expect = 2.130e-7
Identity = 21/54 (38.89%), Postives = 32/54 (59.26%), Query Frame = 1

Query:   70 RNYIDHLPTELFFHIFKFINIKDLLSCSQVSKSWHSLSNDDLVWTSKCRQITFL 231
            R++I  LP EL   +  ++  KDLL  +Q  +SW  L +D+L+W  KCR+   L
Sbjct:  889 RDFISLLPRELALFVLSYLEPKDLLRAAQTCRSWRFLCDDNLLWKEKCRKAQIL 942

[Close]

BLAST of F-box domain-containing protein vs. Ensembl Zebrafish
Match: fbxw7 (F-box and WD repeat domain containing 7 [Source:ZFIN;Acc:ZDB-GENE-090313-79])

HSP 1 Score: 50.8322 bits (120), Expect = 8.318e-7
Identity = 20/50 (40.00%), Postives = 31/50 (62.00%), Query Frame = 1

Query:   70 RNYIDHLPTELFFHIFKFINIKDLLSCSQVSKSWHSLSNDDLVWTSKCRQ 219
            R++I  LP EL  H+  F+  KDLL  +Q  + W  L+ D+L+W  KC++
Sbjct:  115 RDFISLLPRELALHVLSFLEPKDLLQAAQTCRYWRILAEDNLLWKEKCKE 164

[Close]

BLAST of F-box domain-containing protein vs. Ensembl Zebrafish
Match: fbxw7 (F-box and WD repeat domain containing 7 [Source:ZFIN;Acc:ZDB-GENE-090313-79])

HSP 1 Score: 50.8322 bits (120), Expect = 9.831e-7
Identity = 20/50 (40.00%), Postives = 31/50 (62.00%), Query Frame = 1

Query:   70 RNYIDHLPTELFFHIFKFINIKDLLSCSQVSKSWHSLSNDDLVWTSKCRQ 219
            R++I  LP EL  H+  F+  KDLL  +Q  + W  L+ D+L+W  KC++
Sbjct:  331 RDFISLLPRELALHVLSFLEPKDLLQAAQTCRYWRILAEDNLLWKEKCKE 380

[Close]

BLAST of F-box domain-containing protein vs. Ensembl Zebrafish
Match: fbxw8 (F-box and WD repeat domain containing 8 [Source:NCBI gene;Acc:100136859])

HSP 1 Score: 47.7506 bits (112), Expect = 7.303e-6
Identity = 39/116 (33.62%), Postives = 48/116 (41.38%), Query Frame = 1

Query:   22 QFLSELIFLNCNER*TRNYID-HLPTELFFHIFKFINIKDLLSCSQVSKSWHSLSNDDLVWTSKCRQITFLKTKEF----------YRCR-PYNYYKPNWITLGGQMRICSPKLSK 333
            Q L E    + NE     + D  LP EL   IF F+   DL  C+QVSKSW  L+ D+L+W   C    F K                CR   N  K NW    G +R    +L K
Sbjct:  116 QKLLEQFLQDLNEVNDIPFFDVELPYELALKIFNFLGRTDLGRCAQVSKSWKVLAEDELLWYRLCLVEGFQKGANISDSPCWKSTVRDCRNTENAVKSNWKNRVGSIRHLQYELGK 231

[Close]

BLAST of F-box domain-containing protein vs. Ensembl Xenopus
Match: ENSXETT00000008142.1 (F-box and WD repeat domain containing 7 [Source:NCBI gene;Acc:100145670])

HSP 1 Score: 48.521 bits (114), Expect = 5.965e-6
Identity = 19/50 (38.00%), Postives = 32/50 (64.00%), Query Frame = 1

Query:   70 RNYIDHLPTELFFHIFKFINIKDLLSCSQVSKSWHSLSNDDLVWTSKCRQ 219
            R++I  LP EL  ++  F++ KDLL  +Q  + W  L+ D+L+W  KC++
Sbjct:  185 RDFISLLPKELALYVLSFLDPKDLLQAAQTCRYWRILAEDNLLWREKCKE 234

[Close]

BLAST of F-box domain-containing protein vs. Ensembl Xenopus
Match: ENSXETT00000008111.1 (F-box and WD repeat domain containing 7 [Source:NCBI gene;Acc:100145670])

HSP 1 Score: 48.521 bits (114), Expect = 6.834e-6
Identity = 19/50 (38.00%), Postives = 32/50 (64.00%), Query Frame = 1

Query:   70 RNYIDHLPTELFFHIFKFINIKDLLSCSQVSKSWHSLSNDDLVWTSKCRQ 219
            R++I  LP EL  ++  F++ KDLL  +Q  + W  L+ D+L+W  KC++
Sbjct:   85 RDFISLLPKELALYVLSFLDPKDLLQAAQTCRYWRILAEDNLLWREKCKE 134

[Close]

BLAST of F-box domain-containing protein vs. Ensembl Xenopus
Match: ENSXETT00000008122.1 (F-box and WD repeat domain containing 7 [Source:NCBI gene;Acc:100145670])

HSP 1 Score: 48.521 bits (114), Expect = 7.148e-6
Identity = 19/50 (38.00%), Postives = 32/50 (64.00%), Query Frame = 1

Query:   70 RNYIDHLPTELFFHIFKFINIKDLLSCSQVSKSWHSLSNDDLVWTSKCRQ 219
            R++I  LP EL  ++  F++ KDLL  +Q  + W  L+ D+L+W  KC++
Sbjct:  123 RDFISLLPKELALYVLSFLDPKDLLQAAQTCRYWRILAEDNLLWREKCKE 172

[Close]

BLAST of F-box domain-containing protein vs. Ensembl Xenopus
Match: ENSXETT00000008237.1 (F-box and WD repeat domain containing 7 [Source:NCBI gene;Acc:100145670])

HSP 1 Score: 48.1358 bits (113), Expect = 9.644e-6
Identity = 19/50 (38.00%), Postives = 32/50 (64.00%), Query Frame = 1

Query:   70 RNYIDHLPTELFFHIFKFINIKDLLSCSQVSKSWHSLSNDDLVWTSKCRQ 219
            R++I  LP EL  ++  F++ KDLL  +Q  + W  L+ D+L+W  KC++
Sbjct:  274 RDFISLLPKELALYVLSFLDPKDLLQAAQTCRYWRILAEDNLLWREKCKE 323

[Close]

BLAST of F-box domain-containing protein vs. Ensembl Mouse
Match: Fbxw8 (F-box and WD-40 domain protein 8 [Source:MGI Symbol;Acc:MGI:1923041])

HSP 1 Score: 49.6766 bits (117), Expect = 2.255e-6
Identity = 23/58 (39.66%), Postives = 36/58 (62.07%), Query Frame = 1

Query:   88 LPTELFFHIFKFINIKDLLSCSQVSKSWHSLSNDDLVWTSKCRQITFLKTKEF--YRC 255
            LP EL  +IF+++N ++L  C+QVSK+W  ++ D+++W   CRQ   L    F  Y C
Sbjct:  119 LPYELAINIFQYLNRRELGLCAQVSKTWKVIAEDEVLWYRLCRQEGHLPHSRFSDYTC 176

[Close]

BLAST of F-box domain-containing protein vs. Ensembl Mouse
Match: Fbxw7 (F-box and WD-40 domain protein 7 [Source:MGI Symbol;Acc:MGI:1354695])

HSP 1 Score: 48.1358 bits (113), Expect = 6.954e-6
Identity = 19/50 (38.00%), Postives = 31/50 (62.00%), Query Frame = 1

Query:   70 RNYIDHLPTELFFHIFKFINIKDLLSCSQVSKSWHSLSNDDLVWTSKCRQ 219
            R++I  LP EL  ++  F+  KDLL  +Q  + W  L+ D+L+W  KC++
Sbjct:  200 RDFISLLPKELALYVLSFLEPKDLLQAAQTCRYWRILAEDNLLWREKCKE 249

[Close]

BLAST of F-box domain-containing protein vs. Ensembl Mouse
Match: Fbxw7 (F-box and WD-40 domain protein 7 [Source:MGI Symbol;Acc:MGI:1354695])

HSP 1 Score: 48.1358 bits (113), Expect = 6.999e-6
Identity = 19/50 (38.00%), Postives = 31/50 (62.00%), Query Frame = 1

Query:   70 RNYIDHLPTELFFHIFKFINIKDLLSCSQVSKSWHSLSNDDLVWTSKCRQ 219
            R++I  LP EL  ++  F+  KDLL  +Q  + W  L+ D+L+W  KC++
Sbjct:  160 RDFISLLPKELALYVLSFLEPKDLLQAAQTCRYWRILAEDNLLWREKCKE 209

[Close]

BLAST of F-box domain-containing protein vs. UniProt/SwissProt
Match: sp|P0DL28|FBXW8_RAT (F-box/WD repeat-containing protein 8 OS=Rattus norvegicus OX=10116 GN=Fbxw8 PE=1 SV=1)

HSP 1 Score: 55.8398 bits (133), Expect = 1.450e-7
Identity = 28/85 (32.94%), Postives = 46/85 (54.12%), Query Frame = 1

Query:   85 HLPTELFFHIFKFINIKDLLSCSQVSKSWHSLSNDDLVWTSKCRQITFLKTKEF--YRC---------RPYNYYKPNWITLGGQM 306
            HLP EL  +IF+++N ++L  C+QVSK+W  ++ D+++W   CRQ   L    F  Y C          P +  +P+W+   G +
Sbjct:  116 HLPYELAINIFQYLNRRELGLCAQVSKTWKVIAEDEVLWYRLCRQEGHLPHSRFSDYTCWKLILQECLAPVHLIRPSWMNRKGAV 200

[Close]

BLAST of F-box domain-containing protein vs. UniProt/SwissProt
Match: sp|Q9VZF4|FBXW7_DROME (F-box/WD repeat-containing protein 7 OS=Drosophila melanogaster OX=7227 GN=ago PE=1 SV=1)

HSP 1 Score: 52.373 bits (124), Expect = 2.135e-6
Identity = 21/54 (38.89%), Postives = 32/54 (59.26%), Query Frame = 1

Query:   70 RNYIDHLPTELFFHIFKFINIKDLLSCSQVSKSWHSLSNDDLVWTSKCRQITFL 231
            R++I  LP EL   +  ++  KDLL  +Q  +SW  L +D+L+W  KCR+   L
Sbjct:  889 RDFISLLPRELALFVLSYLEPKDLLRAAQTCRSWRFLCDDNLLWKEKCRKAQIL 942

[Close]

BLAST of F-box domain-containing protein vs. TrEMBL
Match: A0A0R3UI21 (Uncharacterized protein OS=Mesocestoides corti OX=53468 GN=MCOS_LOCUS7036 PE=4 SV=1)

HSP 1 Score: 60.077 bits (144), Expect = 1.679e-6
Identity = 27/73 (36.99%), Postives = 42/73 (57.53%), Query Frame = 1

Query:   70 RNYIDHLPTELFFHIFKFINIKDLLSCSQVSKSWHSLSNDDLVWTSKCRQITFLK--TKEFYRCRPYNYYKPN 282
            R++I HLP E+   I  ++  KDLL C+QVS+ WH++S D L+W   C+Q+   +   KE +   P   +  N
Sbjct:   83 RDFISHLPYEIAIKILSYLGPKDLLQCAQVSRVWHAVSEDPLLWKHICQQLNIPRCFPKEMHHLSPRLEFSTN 155

[Close]

BLAST of F-box domain-containing protein vs. Ensembl Cavefish
Match: fbxw7 (F-box and WD repeat domain containing 7 [Source:NCBI gene;Acc:103046885])

HSP 1 Score: 49.6766 bits (117), Expect = 1.846e-6
Identity = 20/50 (40.00%), Postives = 31/50 (62.00%), Query Frame = 1

Query:   70 RNYIDHLPTELFFHIFKFINIKDLLSCSQVSKSWHSLSNDDLVWTSKCRQ 219
            R++I  LP EL  ++  F+  KDLL  +Q  + W  L+ D+L+W  KCR+
Sbjct:  119 RDFISLLPKELALYVLSFLEPKDLLQAAQTCRYWRILAEDNLLWREKCRE 168

[Close]

BLAST of F-box domain-containing protein vs. Ensembl Cavefish
Match: fbxw7 (F-box and WD repeat domain containing 7 [Source:NCBI gene;Acc:103046885])

HSP 1 Score: 48.9062 bits (115), Expect = 3.316e-6
Identity = 20/50 (40.00%), Postives = 31/50 (62.00%), Query Frame = 1

Query:   70 RNYIDHLPTELFFHIFKFINIKDLLSCSQVSKSWHSLSNDDLVWTSKCRQ 219
            R++I  LP EL  ++  F+  KDLL  +Q  + W  L+ D+L+W  KCR+
Sbjct:  361 RDFISLLPKELALYVLSFLEPKDLLQAAQTCRYWRILAEDNLLWREKCRE 410

[Close]

BLAST of F-box domain-containing protein vs. Ensembl Sea Lamprey
Match: ENSPMAT00000008862.1 (pep scaffold:Pmarinus_7.0:GL476579:86637:114999:1 gene:ENSPMAG00000008000.1 transcript:ENSPMAT00000008862.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 48.1358 bits (113), Expect = 9.487e-7
Identity = 19/50 (38.00%), Postives = 31/50 (62.00%), Query Frame = 1

Query:   70 RNYIDHLPTELFFHIFKFINIKDLLSCSQVSKSWHSLSNDDLVWTSKCRQ 219
            R++I  LP EL  ++  F+  +DLL  +Q  + W  L+ D+L+W  KCR+
Sbjct:  131 RDFISLLPKELALYVLSFLEPRDLLRAAQTCRYWRILAEDNLLWREKCRE 180

[Close]

BLAST of F-box domain-containing protein vs. Ensembl Sea Lamprey
Match: fbxw2 (F-box and WD repeat domain containing 2 [Source:ZFIN;Acc:ZDB-GENE-050522-303])

HSP 1 Score: 45.0542 bits (105), Expect = 8.720e-6
Identity = 20/54 (37.04%), Postives = 35/54 (64.81%), Query Frame = 1

Query:   70 RNYIDHLPTELFFHIFKFINIKDLLSCSQVSKSWHSLSND-DLVWTSKCRQITF 228
            R+++  LP EL FH+ K+++ + LL+C  V +SW+ + N    VW S CR++ +
Sbjct:   54 RDFLRLLPLELSFHLLKWLDPRSLLTCCLVCRSWNKVVNGCSEVWLSACRELGW 107

[Close]

BLAST of F-box domain-containing protein vs. Ensembl Nematostella
Match: EDO42306 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S1V2])

HSP 1 Score: 47.7506 bits (112), Expect = 2.667e-6
Identity = 21/58 (36.21%), Postives = 31/58 (53.45%), Query Frame = 1

Query:   61 R*TRNYIDHLPTELFFHIFKFINIKDLLSCSQVSKSWHSLSNDDLVWTSKCRQITFLK 234
            R  R++I  LP EL  ++  F+   DLL  +Q  K W  L  D+L+W  KCR+    +
Sbjct:  171 RFQRDFISLLPRELALYVLSFLEPSDLLHAAQTCKCWQILCEDNLLWKQKCREAGITE 228

[Close]

BLAST of F-box domain-containing protein vs. Ensembl Medaka
Match: ENSORLT00000003546.2 (F-box/WD repeat-containing protein 7 [Source:NCBI gene;Acc:101171440])

HSP 1 Score: 49.6766 bits (117), Expect = 1.611e-6
Identity = 23/75 (30.67%), Postives = 38/75 (50.67%), Query Frame = 1

Query:   70 RNYIDHLPTELFFHIFKFINIKDLLSCSQVSKSWHSLSNDDLVWTSKCRQITFLKTKEFYR---CRPYNYYKPNW 285
            R++I  LP EL  ++  F+  +DLL  +Q  + W  L+ D+L+W  KCR+    ++  + R    RP       W
Sbjct:  122 RDFISLLPKELALYVLTFLPPRDLLRAAQTCRYWRILAEDNLLWREKCREEGISESASYRRRESVRPSAAAVSPW 196

[Close]

BLAST of F-box domain-containing protein vs. Ensembl Medaka
Match: fbxw7 (F-box and WD repeat domain containing 7 [Source:NCBI gene;Acc:101155087])

HSP 1 Score: 49.6766 bits (117), Expect = 1.697e-6
Identity = 20/50 (40.00%), Postives = 31/50 (62.00%), Query Frame = 1

Query:   70 RNYIDHLPTELFFHIFKFINIKDLLSCSQVSKSWHSLSNDDLVWTSKCRQ 219
            R++I  LP EL  ++  F+  KDLL  +Q  + W  L+ D+L+W  KCR+
Sbjct:  124 RDFISLLPKELALYVLSFLEPKDLLQAAQTCRYWRILAEDNLLWREKCRE 173

[Close]

BLAST of F-box domain-containing protein vs. Ensembl Medaka
Match: fbxw7 (F-box and WD repeat domain containing 7 [Source:NCBI gene;Acc:101155087])

HSP 1 Score: 49.2914 bits (116), Expect = 2.645e-6
Identity = 20/50 (40.00%), Postives = 31/50 (62.00%), Query Frame = 1

Query:   70 RNYIDHLPTELFFHIFKFINIKDLLSCSQVSKSWHSLSNDDLVWTSKCRQ 219
            R++I  LP EL  ++  F+  KDLL  +Q  + W  L+ D+L+W  KCR+
Sbjct:  349 RDFISLLPKELALYVLSFLEPKDLLQAAQTCRYWRILAEDNLLWREKCRE 398

[Close]

BLAST of F-box domain-containing protein vs. Planmine SMEST
Match: SMESG000065926.1 (SMESG000065926.1)

HSP 1 Score: 196.438 bits (498), Expect = 6.496e-58
Identity = 93/110 (84.55%), Postives = 105/110 (95.45%), Query Frame = 3

Query:  252 MSTLQLLQTELDHARRSNADLLTQIIQMTREIQQLKATWSDPTKMKAIYHRLTAAQKGWAEERQLNQSLRTQIRGLEVALAVCREGEAVTYPLVFAPSQIVPKNPQLADQ 581
            MS+LQ+LQTEL+HAR+SNADLLT+IIQ+TREIQQ+KATWSDPTKM+A+YHRLTAAQKGWAEERQLNQSLRTQIRGLEVALA CREGEAVTYPLVFAPSQ+  K+ Q A+Q
Sbjct:    1 MSSLQILQTELEHARQSNADLLTKIIQITREIQQMKATWSDPTKMRAVYHRLTAAQKGWAEERQLNQSLRTQIRGLEVALAACREGEAVTYPLVFAPSQMPQKSIQSAEQ 110

[Close]

BLAST of F-box domain-containing protein vs. Planmine SMEST
Match: SMESG000065926.1 (SMESG000065926.1)

HSP 1 Score: 196.052 bits (497), Expect = 7.872e-58
Identity = 93/110 (84.55%), Postives = 105/110 (95.45%), Query Frame = 3

Query:  252 MSTLQLLQTELDHARRSNADLLTQIIQMTREIQQLKATWSDPTKMKAIYHRLTAAQKGWAEERQLNQSLRTQIRGLEVALAVCREGEAVTYPLVFAPSQIVPKNPQLADQ 581
            MS+LQ+LQTEL+HAR+SNADLLT+IIQ+TREIQQ+KATWSDPTKM+A+YHRLTAAQKGWAEERQLNQSLRTQIRGLEVALA CREGEAVTYPLVFAPSQ+  K+ Q A+Q
Sbjct:    1 MSSLQILQTELEHARQSNADLLTKIIQITREIQQMKATWSDPTKMRAVYHRLTAAQKGWAEERQLNQSLRTQIRGLEVALAACREGEAVTYPLVFAPSQMPQKSIQSAEQ 110

[Close]

BLAST of F-box domain-containing protein vs. Planmine SMEST
Match: SMESG000004174.1 (SMESG000004174.1)

HSP 1 Score: 189.119 bits (479), Expect = 5.113e-57
Identity = 90/117 (76.92%), Postives = 103/117 (88.03%), Query Frame = 3

Query:  252 MSTLQLLQTELDHARRSNADLLTQIIQMTREIQQLKATWSDPTKMKAIYHRLTAAQKGWAEERQLNQSLRTQIRGLEVALAVCREGEAVTYPLVFAPSQIVPKNPQLADQPTNQPVN 602
            MS+LQLLQ EL+ AR+SNA+LL QI+Q+TRE QQLKATW DPTKM+A+YHRLTAAQKGW EERQLNQSLRTQIRGLEVALAVCREGEAVTYPL+FAP+Q+    PQ   +PT QP+N
Sbjct:    1 MSSLQLLQAELERARQSNAELLAQILQLTREAQQLKATWVDPTKMRAVYHRLTAAQKGWIEERQLNQSLRTQIRGLEVALAVCREGEAVTYPLIFAPTQM----PQTTTKPTEQPIN 113

[Close]

BLAST of F-box domain-containing protein vs. Planmine SMEST
Match: SMESG000067481.1 (SMESG000067481.1)

HSP 1 Score: 177.948 bits (450), Expect = 9.533e-55
Identity = 84/105 (80.00%), Postives = 97/105 (92.38%), Query Frame = 3

Query:  252 MSTLQLLQTELDHARRSNADLLTQIIQMTREIQQLKATWSDPTKMKAIYHRLTAAQKGWAEERQLNQSLRTQIRGLEVALAVCREGEAVTYPLVFAPSQIVPKNP 566
            M+T QLLQTELDHARR+NA+LL QI+Q+TRE+QQ+K+TWSDP K KA+Y RLTAAQKGW EERQLNQSLRTQIRGLEVALAVCREGEAVTYPL+FAPSQ+  ++P
Sbjct:    1 MTTTQLLQTELDHARRTNANLLDQIVQITREVQQIKSTWSDPKKTKALYLRLTAAQKGWVEERQLNQSLRTQIRGLEVALAVCREGEAVTYPLIFAPSQLPYRDP 105

[Close]

BLAST of F-box domain-containing protein vs. Planmine SMEST
Match: SMESG000038196.1 (SMESG000038196.1)

HSP 1 Score: 182.57 bits (462), Expect = 2.705e-54
Identity = 87/129 (67.44%), Postives = 105/129 (81.40%), Query Frame = 3

Query:  213 PPDYIPKDKGVLSMSTLQLLQTELDHARRSNADLLTQIIQMTREIQQLKATWSDPTKMKAIYHRLTAAQKGWAEERQLNQSLRTQIRGLEVALAVCREGEAVTYPLVFAPSQIVPKNPQLADQPTNQPV 599
            PP  + K  G  +MS+L++LQTELDH+R++N +LL QIIQ+TRE+Q +KA W+DP K K IYHRLTAAQKGW EERQLNQSLRTQIRGLEVALAVCREGEAVTYPL+FAP+Q+    PQ   +P  QP+
Sbjct:   67 PPHPVGKGAGA-AMSSLRILQTELDHSRQANTELLAQIIQLTREMQHMKAIWTDPAKTKTIYHRLTAAQKGWGEERQLNQSLRTQIRGLEVALAVCREGEAVTYPLIFAPTQM----PQTTTKPAEQPI 190

The following BLAST results are available for this feature:

BLAST of F-box domain-containing protein vs. Ensembl Human
BLAST of F-box domain-containing protein vs. Ensembl Celegans
BLAST of F-box domain-containing protein vs. Ensembl Fly
BLAST of F-box domain-containing protein vs. Ensembl Zebrafish
BLAST of F-box domain-containing protein vs. Ensembl Xenopus
BLAST of F-box domain-containing protein vs. Ensembl Mouse
BLAST of F-box domain-containing protein vs. UniProt/SwissProt
BLAST of F-box domain-containing protein vs. TrEMBL
BLAST of F-box domain-containing protein vs. Ensembl Cavefish
BLAST of F-box domain-containing protein vs. Ensembl Sea Lamprey
BLAST of F-box domain-containing protein vs. Ensembl Yeast
BLAST of F-box domain-containing protein vs. Ensembl Nematostella
BLAST of F-box domain-containing protein vs. Ensembl Medaka
BLAST of F-box domain-containing protein vs. Planmine SMEST

BLAST of F-box domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 1


Match Name	E-value	Identity	Description
FBXW7	9.247e-6	38.00	F-box and WD repeat domain containing 7 [Source:HG...	[more]

BLAST of F-box domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of F-box domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 3


Match Name	E-value	Identity	Description
ago	2.130e-7	38.89	gene:FBgn0041171 transcript:FBtr0073246	[more]
ago	2.130e-7	38.89	gene:FBgn0041171 transcript:FBtr0073245	[more]
ago	2.130e-7	38.89	gene:FBgn0041171 transcript:FBtr0073247	[more]

BLAST of F-box domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 3


Match Name	E-value	Identity	Description
fbxw7	8.318e-7	40.00	F-box and WD repeat domain containing 7 [Source:ZF...	[more]
fbxw7	9.831e-7	40.00	F-box and WD repeat domain containing 7 [Source:ZF...	[more]
fbxw8	7.303e-6	33.62	F-box and WD repeat domain containing 8 [Source:NC...	[more]

BLAST of F-box domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 4


Match Name	E-value	Identity	Description
ENSXETT00000008142.1	5.965e-6	38.00	F-box and WD repeat domain containing 7 [Source:NC...	[more]
ENSXETT00000008111.1	6.834e-6	38.00	F-box and WD repeat domain containing 7 [Source:NC...	[more]
ENSXETT00000008122.1	7.148e-6	38.00	F-box and WD repeat domain containing 7 [Source:NC...	[more]
ENSXETT00000008237.1	9.644e-6	38.00	F-box and WD repeat domain containing 7 [Source:NC...	[more]

BLAST of F-box domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 3


Match Name	E-value	Identity	Description
Fbxw8	2.255e-6	39.66	F-box and WD-40 domain protein 8 [Source:MGI Symbo...	[more]
Fbxw7	6.954e-6	38.00	F-box and WD-40 domain protein 7 [Source:MGI Symbo...	[more]
Fbxw7	6.999e-6	38.00	F-box and WD-40 domain protein 7 [Source:MGI Symbo...	[more]

BLAST of F-box domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 2


Match Name	E-value	Identity	Description
sp\|P0DL28\|FBXW8_RAT	1.450e-7	32.94	F-box/WD repeat-containing protein 8 OS=Rattus nor...	[more]
sp\|Q9VZF4\|FBXW7_DROME	2.135e-6	38.89	F-box/WD repeat-containing protein 7 OS=Drosophila...	[more]

BLAST of F-box domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 1


Match Name	E-value	Identity	Description
A0A0R3UI21	1.679e-6	36.99	Uncharacterized protein OS=Mesocestoides corti OX=...	[more]

BLAST of F-box domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 2


Match Name	E-value	Identity	Description
fbxw7	1.846e-6	40.00	F-box and WD repeat domain containing 7 [Source:NC...	[more]
fbxw7	3.316e-6	40.00	F-box and WD repeat domain containing 7 [Source:NC...	[more]

BLAST of F-box domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 2


Match Name	E-value	Identity	Description
ENSPMAT00000008862.1	9.487e-7	38.00	pep scaffold:Pmarinus_7.0:GL476579:86637:114999:1 ...	[more]
fbxw2	8.720e-6	37.04	F-box and WD repeat domain containing 2 [Source:ZF...	[more]

BLAST of F-box domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of F-box domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1


Match Name	E-value	Identity	Description
EDO42306	2.667e-6	36.21	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]

BLAST of F-box domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 3


Match Name	E-value	Identity	Description
ENSORLT00000003546.2	1.611e-6	30.67	F-box/WD repeat-containing protein 7 [Source:NCBI ...	[more]
fbxw7	1.697e-6	40.00	F-box and WD repeat domain containing 7 [Source:NC...	[more]
fbxw7	2.645e-6	40.00	F-box and WD repeat domain containing 7 [Source:NC...	[more]

BLAST of F-box domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5


Match Name	E-value	Identity	Description
SMESG000065926.1	6.496e-58	84.55	SMESG000065926.1	[more]
SMESG000065926.1	7.872e-58	84.55	SMESG000065926.1	[more]
SMESG000004174.1	5.113e-57	76.92	SMESG000004174.1	[more]
SMESG000067481.1	9.533e-55	80.00	SMESG000067481.1	[more]
SMESG000038196.1	2.705e-54	67.44	SMESG000038196.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence
protein sequence

transcript sequence

>SMED30023758 ID=SMED30023758|Name=F-box domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=812bp
TGATATACAAATTTATGACTGCAATTTTTGTCTGAATTAATATTTCTGAA
CTGCAATGAAAGATAAACCAGGAATTACATTGATCATCTTCCAACAGAAT
TATTTTTTCATATCTTCAAATTTATTAACATTAAAGATTTATTGTCCTGT
TCTCAGGTATCTAAAAGTTGGCACAGTTTATCAAATGATGATTTGGTTTG
GACAAGTAAATGCCGCCAGATTACATTCCTAAAGACAAAGGAGTTCTATC
GATGTCGACCCTACAATTACTACAAACCGAACTGGATCACGCTAGGCGGT
CAAATGCGGATCTGCTCACCCAAATTATCCAAATGACCCGAGAAATACAG
CAATTAAAGGCCACCTGGTCAGATCCGACAAAAATGAAAGCTATTTACCA
CAGACTCACGGCGGCACAGAAAGGTTGGGCAGAAGAGCGACAGCTCAATC
AGAGCTTGCGCACCCAAATCAGAGGATTGGAAGTCGCCCTAGCGGTCTGT
CGAGAGGGGGAAGCGGTAACGTACCCGCTCGTGTTCGCCCCGTCCCAGAT
AGTGCCAAAGAACCCTCAGTTAGCAGATCAACCTACTAATCAGCCCGTAA
ATCGTCGGCCAGGACGCAAAGAGCGTGCTCGACGCAGAGCAGCCAATCCA
TCGCATTAACTCACGTTGAATCCCTACATGTGTTAATTTCAATAAATGTG
TTTTAGTCAAATCATTTCTCATAATAAAGATAGATAGGTGTCAATCGGAA
CGCTTACGACCATCTTTCCACCTGTAAACATGAACACTCTTACGCATGGG
ACAGAGCTAAGC

protein sequence of SMED30023758-orf-1

>SMED30023758-orf-1 ID=SMED30023758-orf-1|Name=SMED30023758-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=150bp
MPPDYIPKDKGVLSMSTLQLLQTELDHARRSNADLLTQIIQMTREIQQLK
ATWSDPTKMKAIYHRLTAAQKGWAEERQLNQSLRTQIRGLEVALAVCREG
EAVTYPLVFAPSQIVPKNPQLADQPTNQPVNRRPGRKERARRRAANPSH*

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0000025	nervous system

Vocabulary: **INTERPRO**
Term	Definition
IPR001810	F-box_dom

Vocabulary: **molecular function**
Term	Definition
GO:0005515	protein binding
GO:0004842	ubiquitin-protein transferase activity

Vocabulary: **cellular component**
Term	Definition
GO:0005737	cytoplasm
GO:0005794	Golgi apparatus
GO:0005829	cytosol
GO:0019005	SCF ubiquitin ligase complex
GO:0031467	Cul7-RING ubiquitin ligase complex
GO:0048471	perinuclear region of cytoplasm
GO:1990393	3M complex

Vocabulary: **biological process**
Term	Definition
GO:0007030	Golgi organization
GO:0008283	cell proliferation
GO:0016567	protein ubiquitination
GO:0050775	positive regulation of dendrite morphogenesis
GO:0060712	spongiotrophoblast layer development
GO:0060716	labyrinthine layer blood vessel development

Main menu

F-box domain-containing protein

Zeng et. al., 2018

▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Davies et. al., 2017

PAGE et. al., 2020

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 10

Owlery

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