SMED30023745
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30023745 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 5
Alignments
SMED30023745 aligns in the following genomic locations:
Homology
BLAST of SMED30023745 vs. Ensembl Human
Match: NAE1 (NEDD8 activating enzyme E1 subunit 1 [Source:HGNC Symbol;Acc:HGNC:621]) HSP 1 Score: 393.66 bits (1010), Expect = 3.724e-129 Identity = 207/540 (38.33%), Postives = 328/540 (60.74%), Query Frame = -2
Query: 423 KLLEKTKRYDRQIRLWGENGQIALENAKVCLIGSGCIGTEILKNLILPGIGSFTIVDDSVVTEEDLSNNFFVQKSDLNQSRAEVTARLLRELNEEVIVNYIEDSVESLMFRNPEYFLEFSVVIACGLNNKDLVDLDRLLYPENIGLIVCHSIGMVANLRVIIQEHPVVEAKPDNIKPDLRLDKPLWEFVEFCDSQNLHEMSYKELAYCPWIIILFKYITSFQSKF-AKWPDSYKDKQILRKMINEGRXXXXXXXXXXXXXXXSQYLDSFEEAVKSIPTTVFLSEIPINVRTLLEDSRCIDINRESKSFWILVAALKDFIRSEGNGSLPLRGEIPDMTADTGRYVAIQKIYRQASEKDIIHLEKHVYRICQTF-QIPVTDELNRNSIARFARNCQHIQILSSYSLERELKTSPTHTEELVCP---ETDELSWYLALRAAVSFYTVERRWPGQRQIDSEIDVPHLRKLLQ---QDMNACVKFKDASVRDDFLLEMCRFSGVQLHSVCAFIAGIAAQEVIKLITGQYVPVMQPLMYNAIRQTT 2018
KLL K ++YDRQ+RLWG++GQ ALE+A VCLI + GTEILKNL+LPGIGSFTI+D + V+ ED NNFF+Q+S + ++RAE L+ELN +V +++E+S E+L+ +P +F F+VV+A L + L +L+ I L++C + G+V +R+II+EHPV+E+ PDN DLRLDKP E E S +L M K+ ++ PWI+I+ KY+ + S+ + P +YK+K+ R +I +G K E + ++FEEA+K++ T + ++IP ++ + D RCI+I +++ SFWIL ALK+F+ EG G+LP+RG IPDM AD+G+Y+ +Q +YR+ ++KD + HV ++ Q+ Q P + ++ + N ++++ SL E + +E++ +E+ YL LRA F+ + R+PG E D+ L+ L Q+ V V+DD++ E CR+ + H++ AF+ G AAQEVIK+IT Q+V +Y+ + QT+
Sbjct: 6 KLL-KEQKYDRQLRLWGDHGQEALESAHVCLINATATGTEILKNLVLPGIGSFTIIDGNQVSGEDAGNNFFLQRSSIGKNRAEAAMEFLQELNSDVSGSFVEESPENLLDNDPSFFCRFTVVVATQLPESTSLRLADVLWNSQIPLLICRTYGLVGYMRIIIKEHPVIESHPDNALEDLRLDKPFPELREHFQSYDLDHMEKKDHSHTPWIVIIAKYLAQWYSETNGRIPKTYKEKEDFRDLIRQG--------ILKNENGAPEDEENFEEAIKNVNTALNTTQIPSSIEDIFNDDRCINITKQTPSFWILARALKEFVAKEGQGNLPVRGTIPDMIADSGKYIKLQNVYREKAKKDAAAVGNHVAKLLQSIGQAP--ESISEKELKLLCSNSAFLRVVRCRSLAEEYGLDTINKDEIISSMDNPDNEIVLYLMLRAVDRFHKQQGRYPGVSNYQVEEDIGKLKSCLTGFLQEYGLSVM-----VKDDYVHEFCRYGAAEPHTIAAFLGGAAAQEVIKIITKQFVIFNNTYIYSGMSQTS 529
BLAST of SMED30023745 vs. Ensembl Human
Match: NAE1 (NEDD8 activating enzyme E1 subunit 1 [Source:HGNC Symbol;Acc:HGNC:621]) HSP 1 Score: 388.652 bits (997), Expect = 3.770e-127 Identity = 207/543 (38.12%), Postives = 328/543 (60.41%), Query Frame = -2
Query: 423 KLLEKTKRYDRQIRLWGENGQIALENAKVCLIGSGCIGTEILKNLILPG---IGSFTIVDDSVVTEEDLSNNFFVQKSDLNQSRAEVTARLLRELNEEVIVNYIEDSVESLMFRNPEYFLEFSVVIACGLNNKDLVDLDRLLYPENIGLIVCHSIGMVANLRVIIQEHPVVEAKPDNIKPDLRLDKPLWEFVEFCDSQNLHEMSYKELAYCPWIIILFKYITSFQSKF-AKWPDSYKDKQILRKMINEGRXXXXXXXXXXXXXXXSQYLDSFEEAVKSIPTTVFLSEIPINVRTLLEDSRCIDINRESKSFWILVAALKDFIRSEGNGSLPLRGEIPDMTADTGRYVAIQKIYRQASEKDIIHLEKHVYRICQTF-QIPVTDELNRNSIARFARNCQHIQILSSYSLERELKTSPTHTEELVCP---ETDELSWYLALRAAVSFYTVERRWPGQRQIDSEIDVPHLRKLLQ---QDMNACVKFKDASVRDDFLLEMCRFSGVQLHSVCAFIAGIAAQEVIKLITGQYVPVMQPLMYNAIRQTT 2018
KLL K ++YDRQ+RLWG++GQ ALE+A VCLI + GTEILKNL+LPG IGSFTI+D + V+ ED NNFF+Q+S + ++RAE L+ELN +V +++E+S E+L+ +P +F F+VV+A L + L +L+ I L++C + G+V +R+II+EHPV+E+ PDN DLRLDKP E E S +L M K+ ++ PWI+I+ KY+ + S+ + P +YK+K+ R +I +G K E + ++FEEA+K++ T + ++IP ++ + D RCI+I +++ SFWIL ALK+F+ EG G+LP+RG IPDM AD+G+Y+ +Q +YR+ ++KD + HV ++ Q+ Q P + ++ + N ++++ SL E + +E++ +E+ YL LRA F+ + R+PG E D+ L+ L Q+ V V+DD++ E CR+ + H++ AF+ G AAQEVIK+IT Q+V +Y+ + QT+
Sbjct: 6 KLL-KEQKYDRQLRLWGDHGQEALESAHVCLINATATGTEILKNLVLPGNVGIGSFTIIDGNQVSGEDAGNNFFLQRSSIGKNRAEAAMEFLQELNSDVSGSFVEESPENLLDNDPSFFCRFTVVVATQLPESTSLRLADVLWNSQIPLLICRTYGLVGYMRIIIKEHPVIESHPDNALEDLRLDKPFPELREHFQSYDLDHMEKKDHSHTPWIVIIAKYLAQWYSETNGRIPKTYKEKEDFRDLIRQG--------ILKNENGAPEDEENFEEAIKNVNTALNTTQIPSSIEDIFNDDRCINITKQTPSFWILARALKEFVAKEGQGNLPVRGTIPDMIADSGKYIKLQNVYREKAKKDAAAVGNHVAKLLQSIGQAP--ESISEKELKLLCSNSAFLRVVRCRSLAEEYGLDTINKDEIISSMDNPDNEIVLYLMLRAVDRFHKQQGRYPGVSNYQVEEDIGKLKSCLTGFLQEYGLSVM-----VKDDYVHEFCRYGAAEPHTIAAFLGGAAAQEVIKIITKQFVIFNNTYIYSGMSQTS 532
BLAST of SMED30023745 vs. Ensembl Human
Match: NAE1 (NEDD8 activating enzyme E1 subunit 1 [Source:HGNC Symbol;Acc:HGNC:621]) HSP 1 Score: 381.333 bits (978), Expect = 2.202e-124 Identity = 199/529 (37.62%), Postives = 318/529 (60.11%), Query Frame = -2
Query: 423 QIRLWGENGQIALENAKVCLIGSGCIGTEILKNLILPGIGSFTIVDDSVVTEEDLSNNFFVQKSDLNQSRAEVTARLLRELNEEVIVNYIEDSVESLMFRNPEYFLEFSVVIACGLNNKDLVDLDRLLYPENIGLIVCHSIGMVANLRVIIQEHPVVEAKPDNIKPDLRLDKPLWEFVEFCDSQNLHEMSYKELAYCPWIIILFKYITSFQSKF-AKWPDSYKDKQILRKMINEGRXXXXXXXXXXXXXXXSQYLDSFEEAVKSIPTTVFLSEIPINVRTLLEDSRCIDINRESKSFWILVAALKDFIRSEGNGSLPLRGEIPDMTADTGRYVAIQKIYRQASEKDIIHLEKHVYRICQTF-QIPVTDELNRNSIARFARNCQHIQILSSYSLERELKTSPTHTEELVCP---ETDELSWYLALRAAVSFYTVERRWPGQRQIDSEIDVPHLRKLLQ---QDMNACVKFKDASVRDDFLLEMCRFSGVQLHSVCAFIAGIAAQEVIKLITGQYVPVMQPLMYNAIRQTT 1985
+ RLWG++GQ ALE+A VCLI + GTEILKNL+LPGIGSFTI+D + V+ ED NNFF+Q+S + ++RAE L+ELN +V +++E+S E+L+ +P +F F+VV+A L + L +L+ I L++C + G+V +R+II+EHPV+E+ PDN DLRLDKP E E S +L M K+ ++ PWI+I+ KY+ + S+ + P +YK+K+ R +I +G K E + ++FEEA+K++ T + ++IP ++ + D RCI+I +++ SFWIL ALK+F+ EG G+LP+RG IPDM AD+G+Y+ +Q +YR+ ++KD + HV ++ Q+ Q P + ++ + N ++++ SL E + +E++ +E+ YL LRA F+ + R+PG E D+ L+ L Q+ V V+DD++ E CR+ + H++ AF+ G AAQEVIK+IT Q+V +Y+ + QT+
Sbjct: 10 EARLWGDHGQEALESAHVCLINATATGTEILKNLVLPGIGSFTIIDGNQVSGEDAGNNFFLQRSSIGKNRAEAAMEFLQELNSDVSGSFVEESPENLLDNDPSFFCRFTVVVATQLPESTSLRLADVLWNSQIPLLICRTYGLVGYMRIIIKEHPVIESHPDNALEDLRLDKPFPELREHFQSYDLDHMEKKDHSHTPWIVIIAKYLAQWYSETNGRIPKTYKEKEDFRDLIRQG--------ILKNENGAPEDEENFEEAIKNVNTALNTTQIPSSIEDIFNDDRCINITKQTPSFWILARALKEFVAKEGQGNLPVRGTIPDMIADSGKYIKLQNVYREKAKKDAAAVGNHVAKLLQSIGQAP--ESISEKELKLLCSNSAFLRVVRCRSLAEEYGLDTINKDEIISSMDNPDNEIVLYLMLRAVDRFHKQQGRYPGVSNYQVEEDIGKLKSCLTGFLQEYGLSVM-----VKDDYVHEFCRYGAAEPHTIAAFLGGAAAQEVIKIITKQFVIFNNTYIYSGMSQTS 523
BLAST of SMED30023745 vs. Ensembl Human
Match: NAE1 (NEDD8 activating enzyme E1 subunit 1 [Source:HGNC Symbol;Acc:HGNC:621]) HSP 1 Score: 285.804 bits (730), Expect = 9.482e-89 Identity = 155/453 (34.22%), Postives = 260/453 (57.40%), Query Frame = -2
Query: 423 LLRELNEEVIVNYIEDSVESLMFRNPEYFLEFSVVIACGLNNKDLVDLDRLLYPENIGLIVCHSIGMVANLRVIIQEHPVVEAKPDNIKPDLRLDKPLWEFVEFCDSQNLHEMSYKELAYCPWIIILFKYITSFQSKF-AKWPDSYKDKQILRKMINEGRXXXXXXXXXXXXXXXSQYLDSFEEAVKSIPTTVFLSEIPINVRTLLEDSRCIDINRESKSFWILVAALKDFIRSEGNGSLPLRGEIPDMTADTGRYVAIQKIYRQASEKDIIHLEKHVYRICQTF-QIPVTDELNRNSIARFARNCQHIQILSSYSLERELKTSPTHTEELVCP---ETDELSWYLALRAAVSFYTVERRWPGQRQIDSEIDVPHLRKLLQ---QDMNACVKFKDASVRDDFLLEMCRFSGVQLHSVCAFIAGIAAQEVIKLITGQYVPVMQPLMYNAIRQTT 1757
L+ELN +V +++E+S E+L+ +P +F F+VV+A L + L +L+ I L++C + G+V +R+II+EHPV+E+ PDN DLRLDKP E E S +L M K+ ++ PWI+I+ KY+ + S+ + P +YK+K+ R +I +G K E + ++FEEA+K++ T + ++IP ++ + D RCI+I +++ SFWIL ALK+F+ EG G+LP+RG IPDM AD+G+Y+ +Q +YR+ ++KD + HV ++ Q+ Q P + ++ + N ++++ SL E + +E++ +E+ YL LRA F+ + R+PG E D+ L+ L Q+ V V+DD++ E CR+ + H++ AF+ G AAQEVIK+IT Q+V +Y+ + QT+
Sbjct: 3 FLQELNSDVSGSFVEESPENLLDNDPSFFCRFTVVVATQLPESTSLRLADVLWNSQIPLLICRTYGLVGYMRIIIKEHPVIESHPDNALEDLRLDKPFPELREHFQSYDLDHMEKKDHSHTPWIVIIAKYLAQWYSETNGRIPKTYKEKEDFRDLIRQG--------ILKNENGAPEDEENFEEAIKNVNTALNTTQIPSSIEDIFNDDRCINITKQTPSFWILARALKEFVAKEGQGNLPVRGTIPDMIADSGKYIKLQNVYREKAKKDAAAVGNHVAKLLQSIGQAP--ESISEKELKLLCSNSAFLRVVRCRSLAEEYGLDTINKDEIISSMDNPDNEIVLYLMLRAVDRFHKQQGRYPGVSNYQVEEDIGKLKSCLTGFLQEYGLSVM-----VKDDYVHEFCRYGAAEPHTIAAFLGGAAAQEVIKIITKQFVIFNNTYIYSGMSQTS 440
BLAST of SMED30023745 vs. Ensembl Human
Match: NAE1 (NEDD8 activating enzyme E1 subunit 1 [Source:HGNC Symbol;Acc:HGNC:621]) HSP 1 Score: 149.443 bits (376), Expect = 2.344e-41 Identity = 71/162 (43.83%), Postives = 110/162 (67.90%), Query Frame = -2
Query: 1527 LEKTKRYDRQIRLWGENGQIALENAKVCLIGSGCIGTEILKNLILPGIGSFTIVDDSVVTEEDLSNNFFVQKSDLNQSRAEVTARLLRELNEEVIVNYIEDSVESLMFRNPEYFLEFSVVIACGLNNKDLVDLDRLLYPENIGLIVCHSIGMVANLRVIIQE 2012
++ + + RLWG++GQ ALE+A VCLI + GTEILKNL+LPGIGSFTI+D + V+ ED NNFF+Q+S + ++RAE L+ELN +V +++E+S E+L+ +P +F F+VV+A L + L +L+ I L++C + G+V +R+II+E
Sbjct: 1 MDAQQTKTNEARLWGDHGQEALESAHVCLINATATGTEILKNLVLPGIGSFTIIDGNQVSGEDAGNNFFLQRSSIGKNRAEAAMEFLQELNSDVSGSFVEESPENLLDNDPSFFCRFTVVVATQLPESTSLRLADVLWNSQIPLLICRTYGLVGYMRIIIKE 162
BLAST of SMED30023745 vs. Ensembl Celegans
Match: ula-1 (NEDD8-activating enzyme E1 regulatory subunit [Source:UniProtKB/Swiss-Prot;Acc:Q18217]) HSP 1 Score: 275.789 bits (704), Expect = 3.421e-84 Identity = 161/548 (29.38%), Postives = 284/548 (51.82%), Query Frame = -2
Query: 429 LEKTKRYDRQIRLWGENGQIALENAKVCLIGSGCIGTEILKNLILPGIGSFTIVDDSVVTEEDLSNNFFVQKSDLNQSRAEVTARLLRELNEEVIVNYIEDSVESLMFRNPEYFLEFSVVIACGLNNKDLVDLDRLLYPENIGLIVCHSIGMVANLRVIIQEHPVVEAKPDNIKPDLRLDKPLWEFVEFCDSQNLHEMSYKELAYCPWIIILFKYITSFQSKF---AKWPDSYKDKQILRKMINEGRXXXXXXXXXXXXXXXSQYLDSFEEAVKSIPTTVFLSEIPINVRTLLEDSRCIDINRESKSFWILVAALKDFIRSEGNGSLPLRGEIPDMTADTGRYVAIQKIYRQASEKD---IIHLEKHVYRICQTFQIPVTDELNRNSIARFARNCQHIQILSSYSLERELKTSP--------THTEELVCPETDELSWYLALRAAVSFYTVERRWPGQRQIDSEIDVPHLRK----LLQQDMNACVKFKDAS--VRDDFLLEMCRFSGVQLHSVCAFIAGIAAQEVIKLITGQYVPVMQPLMYNAIRQ 2012
+ + RYDRQ+RLWGE GQ ++ + C++GS + TEILK+L+L G+ SF +VDD+ V + D+ NFF+ D+ +SRAE T L ELN V + +L + E FSVV+A N + ++LY + I + G++ +R+ I+EH + + +N +PDLRLD P + +E + NL EM+ ++L + P+I++ FK + F+ + +P + +++ L+ ++ R + + ++F+EA ++ + I +V+++L +C + ++ FW++ AL+ F+ +E N LPLRG +PDMT+D+ RY + ++ + + D ++ L + V + + V D ++ + RF +N I++ L+ +T EE + DE +W L +RA F + R+PG + ID L+K L+++ + F S V D + E+CRF +LH + +++ GIAAQE+IKL T QYVP+ +++ Q
Sbjct: 4 FDPSTRYDRQVRLWGEEGQASIGSTSACVLGSDSLATEILKSLVLAGVQSFYVVDDAKVEQADIGQNFFLHADDIGRSRAEATLEKLTELNPSVSGSASSQPPTALAMEDVEKLTTFSVVVAANQNEEIDTTFAKVLYNIRVPFICIKTFGLIGTIRICIKEHTIANSHEENPRPDLRLDAPFSKLIEMINETNLDEMTLEQLRHTPYILLHFKALEVFRKQRNDPEAFPSTTAERKELQAILMSFRRSSEESGTKDS--------ENFDEAKAAVIRAFQRTTIGSSVKSILSSPQC---STSTRPFWLICEALRRFV-TENNNLLPLRGTLPDMTSDSSRYTRLATLFHEKALSDAQEVLRLTREVEK-----ERGVGDVISDDVCYRFCKNADRIRVQYGDVLDYNEETKAIVEKIRESNIDEETRNQKVDEATWMLLMRAVGRFQKEKGRYPGTNGVPVSIDAQDLKKRVEVLIREALKDEQDFTSISNKVTDTAIAEICRFGAAELHVISSYVGGIAAQEIIKLATNQYVPIDNTFIFDGHTQ 534
BLAST of SMED30023745 vs. Ensembl Celegans
Match: ula-1 (NEDD8-activating enzyme E1 regulatory subunit [Source:UniProtKB/Swiss-Prot;Acc:Q18217]) HSP 1 Score: 275.018 bits (702), Expect = 6.347e-84 Identity = 161/545 (29.54%), Postives = 280/545 (51.38%), Query Frame = -2
Query: 429 LEKTKRYDRQIRLWGENGQIALENAKVCLIGSGCIGTEILKNLILPGIGSFTIVDDSVVTEEDLSNNFFVQKSDLNQSRAEVTARLLRELNEEVIVNYIEDSVESLMFRNPEYFLEFSVVIACGLNNKDLVDLDRLLYPENIGLIVCHSIGMVANLRVIIQEHPVVEAKPDNIKPDLRLDKPLWEFVEFCDSQNLHEMSYKELAYCPWIIILFKYITSFQSKFAKWPDSYKDKQILRKMINEGRXXXXXXXXXXXXXXXSQYLDSFEEAVKSIPTTVFLSEIPINVRTLLEDSRCIDINRESKSFWILVAALKDFIRSEGNGSLPLRGEIPDMTADTGRYVAIQKIYRQASEKD---IIHLEKHVYRICQTFQIPVTDELNRNSIARFARNCQHIQILSSYSLERELKTSP--------THTEELVCPETDELSWYLALRAAVSFYTVERRWPGQRQIDSEIDVPHLRK----LLQQDMNACVKFKDAS--VRDDFLLEMCRFSGVQLHSVCAFIAGIAAQEVIKLITGQYVPVMQPLMYNAIRQ 2012
+ + RYDRQ+RLWGE GQ ++ + C++GS + TEILK+L+L G+ SF +VDD+ V + D+ NFF+ D+ +SRAE T L ELN V + +L + E FSVV+A N + ++LY + I + G++ +R+ I+EH + + +N +PDLRLD P + +E + NL EM+ ++L + P+I++ FK + F+ K P+++ RK + + + + ++ +++ T F +I +V+++L +C + ++ FW++ AL+ F+ +E N LPLRG +PDMT+D+ RY + ++ + + D ++ L + V + + V D ++ + RF +N I++ L+ +T EE + DE +W L +RA F + R+PG + ID L+K L+++ + F S V D + E+CRF +LH + +++ GIAAQE+IKL T QYVP+ +++ Q
Sbjct: 4 FDPSTRYDRQVRLWGEEGQASIGSTSACVLGSDSLATEILKSLVLAGVQSFYVVDDAKVEQADIGQNFFLHADDIGRSRAEATLEKLTELNPSVSGSASSQPPTALAMEDVEKLTTFSVVVAANQNEEIDTTFAKVLYNIRVPFICIKTFGLIGTIRICIKEHTIANSHEENPRPDLRLDAPFSKLIEMINETNLDEMTLEQLRHTPYILLHFKALEVFR-KQRNDPEAFPSTTAERKELQAILMSFRRSSEESGTKDSENFDEAKAAVIRAFQRTTF--QIGSSVKSILSSPQC---STSTRPFWLICEALRRFV-TENNNLLPLRGTLPDMTSDSSRYTRLATLFHEKALSDAQEVLRLTREVEK-----ERGVGDVISDDVCYRFCKNADRIRVQYGDVLDYNEETKAIVEKIRESNIDEETRNQKVDEATWMLLMRAVGRFQKEKGRYPGTNGVPVSIDAQDLKKRVEVLIREALKDEQDFTSISNKVTDTAIAEICRFGAAELHVISSYVGGIAAQEIIKLATNQYVPIDNTFIFDGHTQ 536
BLAST of SMED30023745 vs. Ensembl Celegans
Match: uba-1 (UBA (Human ubiquitin) related [Source:UniProtKB/TrEMBL;Acc:Q27481]) HSP 1 Score: 65.855 bits (159), Expect = 8.701e-11 Identity = 40/108 (37.04%), Postives = 57/108 (52.78%), Query Frame = -2
Query: 1704 NVVKLLEKTKRYDRQIRLWGENGQIALENAKVCLIGSGCIGTEILKNLILPGIGSFTIVDDSVVTEEDLSNNFFVQKSDLNQSRAEVTARLLRELNEEVIVNYIEDSV 2027
N +LL+K Y RQI GE+ + L A V + G G +G EI KNLIL G+ TI D + DLS ++++ +D+ +RA L ELN+ V V D +
Sbjct: 13 NSDELLDKN-LYSRQIYTLGESAMVNLRTASVLISGLGSVGVEIAKNLILGGVRHVTIHDTKLAKWSDLSAQYYLRDADVGHNRATSCYERLAELNDSVNVQVSTDEL 119 HSP 2 Score: 56.9954 bits (136), Expect = 4.360e-8 Identity = 45/165 (27.27%), Postives = 79/165 (47.88%), Query Frame = -2
Query: 1536 KTKRYDRQIRLWGENGQIALENAKVCLIGSGCIGTEILKNLILPGI-----GSFTIVDDSVVTEEDLSNNFFVQKSDLNQSRAEVTARLLRELNEEVIVNYIEDSV--ESLMFRNPEYFLEFSVVIACGLNNKDLVD-LDRLLYPENIGLIVCHSIGMVANLRVI 2006
+ RYD Q ++G Q L + ++G+G IG E+LKNL + G+ G I D + +L+ F ++ D+ ++E AR + N +V + + + V E+ N E+F E + V A L+N D +DR + L+ ++G N +V+
Sbjct: 411 RQSRYDGQAAVFGWPYQECLFRQRWFVVGAGAIGCELLKNLSMMGVACGEGGLIKITDMDQIEISNLNRQFLFRRRDVGGKKSECAARAVTAFNSDVRIEALAERVGLETEHIFNDEFFGELNGV-ANALDNVDARRYMDRRCVYYRLPLLESGTMGTKGNTQVV 574
BLAST of SMED30023745 vs. Ensembl Celegans
Match: uba-1 (UBA (Human ubiquitin) related [Source:UniProtKB/TrEMBL;Acc:Q27481]) HSP 1 Score: 65.855 bits (159), Expect = 8.701e-11 Identity = 40/108 (37.04%), Postives = 57/108 (52.78%), Query Frame = -2
Query: 1704 NVVKLLEKTKRYDRQIRLWGENGQIALENAKVCLIGSGCIGTEILKNLILPGIGSFTIVDDSVVTEEDLSNNFFVQKSDLNQSRAEVTARLLRELNEEVIVNYIEDSV 2027
N +LL+K Y RQI GE+ + L A V + G G +G EI KNLIL G+ TI D + DLS ++++ +D+ +RA L ELN+ V V D +
Sbjct: 13 NSDELLDKN-LYSRQIYTLGESAMVNLRTASVLISGLGSVGVEIAKNLILGGVRHVTIHDTKLAKWSDLSAQYYLRDADVGHNRATSCYERLAELNDSVNVQVSTDEL 119 HSP 2 Score: 56.9954 bits (136), Expect = 4.360e-8 Identity = 45/165 (27.27%), Postives = 79/165 (47.88%), Query Frame = -2
Query: 1536 KTKRYDRQIRLWGENGQIALENAKVCLIGSGCIGTEILKNLILPGI-----GSFTIVDDSVVTEEDLSNNFFVQKSDLNQSRAEVTARLLRELNEEVIVNYIEDSV--ESLMFRNPEYFLEFSVVIACGLNNKDLVD-LDRLLYPENIGLIVCHSIGMVANLRVI 2006
+ RYD Q ++G Q L + ++G+G IG E+LKNL + G+ G I D + +L+ F ++ D+ ++E AR + N +V + + + V E+ N E+F E + V A L+N D +DR + L+ ++G N +V+
Sbjct: 411 RQSRYDGQAAVFGWPYQECLFRQRWFVVGAGAIGCELLKNLSMMGVACGEGGLIKITDMDQIEISNLNRQFLFRRRDVGGKKSECAARAVTAFNSDVRIEALAERVGLETEHIFNDEFFGELNGV-ANALDNVDARRYMDRRCVYYRLPLLESGTMGTKGNTQVV 574
BLAST of SMED30023745 vs. Ensembl Celegans
Match: uba-1 (UBA (Human ubiquitin) related [Source:UniProtKB/TrEMBL;Acc:Q27481]) HSP 1 Score: 65.855 bits (159), Expect = 8.701e-11 Identity = 40/108 (37.04%), Postives = 57/108 (52.78%), Query Frame = -2
Query: 1704 NVVKLLEKTKRYDRQIRLWGENGQIALENAKVCLIGSGCIGTEILKNLILPGIGSFTIVDDSVVTEEDLSNNFFVQKSDLNQSRAEVTARLLRELNEEVIVNYIEDSV 2027
N +LL+K Y RQI GE+ + L A V + G G +G EI KNLIL G+ TI D + DLS ++++ +D+ +RA L ELN+ V V D +
Sbjct: 13 NSDELLDKN-LYSRQIYTLGESAMVNLRTASVLISGLGSVGVEIAKNLILGGVRHVTIHDTKLAKWSDLSAQYYLRDADVGHNRATSCYERLAELNDSVNVQVSTDEL 119 HSP 2 Score: 56.9954 bits (136), Expect = 4.360e-8 Identity = 45/165 (27.27%), Postives = 79/165 (47.88%), Query Frame = -2
Query: 1536 KTKRYDRQIRLWGENGQIALENAKVCLIGSGCIGTEILKNLILPGI-----GSFTIVDDSVVTEEDLSNNFFVQKSDLNQSRAEVTARLLRELNEEVIVNYIEDSV--ESLMFRNPEYFLEFSVVIACGLNNKDLVD-LDRLLYPENIGLIVCHSIGMVANLRVI 2006
+ RYD Q ++G Q L + ++G+G IG E+LKNL + G+ G I D + +L+ F ++ D+ ++E AR + N +V + + + V E+ N E+F E + V A L+N D +DR + L+ ++G N +V+
Sbjct: 411 RQSRYDGQAAVFGWPYQECLFRQRWFVVGAGAIGCELLKNLSMMGVACGEGGLIKITDMDQIEISNLNRQFLFRRRDVGGKKSECAARAVTAFNSDVRIEALAERVGLETEHIFNDEFFGELNGV-ANALDNVDARRYMDRRCVYYRLPLLESGTMGTKGNTQVV 574
BLAST of SMED30023745 vs. Ensembl Fly
Match: APP-BP1 (gene:FBgn0261112 transcript:FBtr0076208) HSP 1 Score: 339.347 bits (869), Expect = 1.445e-108 Identity = 199/539 (36.92%), Postives = 305/539 (56.59%), Query Frame = -2
Query: 435 KLLEKTKRYDRQIRLWGENGQIALENAKVCLIGSGCIGTEILKNLILPGIGSFTIVDDSVVTEEDLSNNFFVQKSDLNQSRAEVTARLLRELNEEVIVNYIEDSVESLMFRNPEYFLEFSVVIACGLNNKDLVDLDRLLYPENIGLIVCHSIGMVANLRVIIQEHPVVEAKPDNIKPDLRLDKPLWEFVEFCDSQNLHEMSYKELAYCPWIIILFKYITSFQSKFA---KWPDSYKDKQILRKMINEGRXXXXXXXXXXXXXXXSQYLDSFEEAVKSIPTTVFLSEIPINVRTLLEDSRCIDINRESKSFWILVAALKDFIRSEGNGSLPLRGEIPDMTADTGRYVAIQKIYRQASEKDIIHLEKHVYRICQTF--QIPV-TDELNRNSIARFARNCQHIQILSSYSLERELKTSPTHTEELVCPETDEL-----SWYLALRAAVSFYTVERRWPGQRQIDSEIDVPHLRKLLQQDMNACVKFKDASVRDDFLLEMCRFSGVQLHSVCAFIAGIAAQEVIKLITGQYVPVMQPLMYNAI 2018
+L +K+K+YDRQIRLWGE+GQ LE A VCL+ +G E K L+LPGIG FT+ D S V EEDL NNFF+ S L +S+A +LL+ELN +V +Y+++S + L+ P +F F +VIA LN + L+ L L+ N+ LI C S+GM+ +R+ I+EH +VEA PDN + DLRL+ P E D E++ K PW+++L KY+ +Q + A + P +YK+K L++ I E + +++EEA+K++ T ++P +++ + ED C +N++S FWI+ ALK F+ E G LPL G +PDMTA+T Y+A+Q IYRQ + +D VY CQ + Q+ + D ++ S+ + + ++ + E + S + L E +EL ++ ALRA F + PG+ + E D+ L+ + + ++ A++ DD L E+CR+ G +LH+V AFI G AAQEVIK+IT QY P+ +YNAI
Sbjct: 10 ELSDKSKKYDRQIRLWGEHGQTLLEAATVCLVNVTAVGCETAKGLVLPGIGGFTVADGSTVKEEDLGNNFFLDSSYLGKSKALACMQLLQELNPDVNGDYVDESADFLLANRPNFFDSFDLVIASNLNEQTLLLLAERLWELNVPLIYCRSLGMLGTIRLQIREHCIVEAHPDNRQFDLRLEHPFDALREHLDGT---EVTSK----VPWLLVLHKYLNVWQKQQADGTQTPRNYKEKNQLKETIREEMKADE---------------ENYEEAIKAVNTAFGAGQVPKSLKAIFEDDACEQLNKKSNVFWIMAKALKHFVIHENEGHLPLPGVLPDMTANTDSYIALQHIYRQQALQDA----DQVYHKCQEYLKQLALPADSIDERSVRLICKEAAGLAVIRGTRIAEEYEKS---SRLLPLVEDNELQGNLTAYNFALRAYERFLSECGNIPGECIV--EQDIGRLKSIAAKMLSDLGMH--ATISDDVLHEICRYGGAELHAVSAFIGGCAAQEVIKIITKQYKPIDNTFIYNAI 515
BLAST of SMED30023745 vs. Ensembl Fly
Match: APP-BP1 (gene:FBgn0261112 transcript:FBtr0331179) HSP 1 Score: 339.347 bits (869), Expect = 1.445e-108 Identity = 199/539 (36.92%), Postives = 305/539 (56.59%), Query Frame = -2
Query: 435 KLLEKTKRYDRQIRLWGENGQIALENAKVCLIGSGCIGTEILKNLILPGIGSFTIVDDSVVTEEDLSNNFFVQKSDLNQSRAEVTARLLRELNEEVIVNYIEDSVESLMFRNPEYFLEFSVVIACGLNNKDLVDLDRLLYPENIGLIVCHSIGMVANLRVIIQEHPVVEAKPDNIKPDLRLDKPLWEFVEFCDSQNLHEMSYKELAYCPWIIILFKYITSFQSKFA---KWPDSYKDKQILRKMINEGRXXXXXXXXXXXXXXXSQYLDSFEEAVKSIPTTVFLSEIPINVRTLLEDSRCIDINRESKSFWILVAALKDFIRSEGNGSLPLRGEIPDMTADTGRYVAIQKIYRQASEKDIIHLEKHVYRICQTF--QIPV-TDELNRNSIARFARNCQHIQILSSYSLERELKTSPTHTEELVCPETDEL-----SWYLALRAAVSFYTVERRWPGQRQIDSEIDVPHLRKLLQQDMNACVKFKDASVRDDFLLEMCRFSGVQLHSVCAFIAGIAAQEVIKLITGQYVPVMQPLMYNAI 2018
+L +K+K+YDRQIRLWGE+GQ LE A VCL+ +G E K L+LPGIG FT+ D S V EEDL NNFF+ S L +S+A +LL+ELN +V +Y+++S + L+ P +F F +VIA LN + L+ L L+ N+ LI C S+GM+ +R+ I+EH +VEA PDN + DLRL+ P E D E++ K PW+++L KY+ +Q + A + P +YK+K L++ I E + +++EEA+K++ T ++P +++ + ED C +N++S FWI+ ALK F+ E G LPL G +PDMTA+T Y+A+Q IYRQ + +D VY CQ + Q+ + D ++ S+ + + ++ + E + S + L E +EL ++ ALRA F + PG+ + E D+ L+ + + ++ A++ DD L E+CR+ G +LH+V AFI G AAQEVIK+IT QY P+ +YNAI
Sbjct: 10 ELSDKSKKYDRQIRLWGEHGQTLLEAATVCLVNVTAVGCETAKGLVLPGIGGFTVADGSTVKEEDLGNNFFLDSSYLGKSKALACMQLLQELNPDVNGDYVDESADFLLANRPNFFDSFDLVIASNLNEQTLLLLAERLWELNVPLIYCRSLGMLGTIRLQIREHCIVEAHPDNRQFDLRLEHPFDALREHLDGT---EVTSK----VPWLLVLHKYLNVWQKQQADGTQTPRNYKEKNQLKETIREEMKADE---------------ENYEEAIKAVNTAFGAGQVPKSLKAIFEDDACEQLNKKSNVFWIMAKALKHFVIHENEGHLPLPGVLPDMTANTDSYIALQHIYRQQALQDA----DQVYHKCQEYLKQLALPADSIDERSVRLICKEAAGLAVIRGTRIAEEYEKS---SRLLPLVEDNELQGNLTAYNFALRAYERFLSECGNIPGECIV--EQDIGRLKSIAAKMLSDLGMH--ATISDDVLHEICRYGGAELHAVSAFIGGCAAQEVIKIITKQYKPIDNTFIYNAI 515
BLAST of SMED30023745 vs. Ensembl Fly
Match: Aos1 (gene:FBgn0029512 transcript:FBtr0082593) HSP 1 Score: 99.3673 bits (246), Expect = 2.909e-22 Identity = 58/161 (36.02%), Postives = 83/161 (51.55%), Query Frame = -2
Query: 1512 YDRQIRLWGENGQIALENAKVCLIGSGCIGTEILKNLILPGIGSFTIVDDSVVTEEDLSNNFFVQKSDLNQSRAEVTARLLRELNEEVIVNYIEDSVESLMFRNPEYFLEFSVVIACGLNNKDLVDLDRLLYPENIGLIVCHSIGMVANLRVIIQEHPVVE 1994
YDRQIRLWG Q L AK+ + G +G EI KN+IL G+ S ++DD VTEED + F V + LN +RAE + R LN V ++ E L + E+F +F VV+ G N++L+ +D + + I G +Q+H VE
Sbjct: 22 YDRQIRLWGLESQKRLRTAKILIAGLCGLGAEITKNIILSGVNSVKLLDDKDVTEEDFCSQFLVPRESLNTNRAEASLTRARALNPMVDISA---DREPLKEKTSEFFGQFDVVVVNGATNEELLRIDTICRDLGVKFIATDVWGTFGFYFASLQKHSYVE 179
BLAST of SMED30023745 vs. Ensembl Fly
Match: Uba1 (gene:FBgn0023143 transcript:FBtr0339517) HSP 1 Score: 64.6994 bits (156), Expect = 2.157e-10 Identity = 45/162 (27.78%), Postives = 86/162 (53.09%), Query Frame = -2
Query: 1533 RYDRQIRLWGENGQIALENAKVCLIGSGCIGTEILKNLILPGIGSFT----IVDDSVVTEEDLSNNFFVQKSDLNQSRAEVTARLLRELNEEVIVNYIEDSV--ESLMFRNPEYFLEFSVVIACGLNNKDL-VDLDRLLYPENIGLIVCHSIGMVANLRVII 1997
RYD QI ++G+ Q L ++K ++G+G IG E+LKN + G+G+ + D ++ + +L+ F + D+ + ++ A ++ +N EV V E V E+ + ++F + V A L+N D + +DR I L+ ++G + N++VI+
Sbjct: 403 RYDSQIAIFGKKFQEKLADSKWFIVGAGAIGCELLKNFGMLGLGTGNGQIFVTDMDLIEKSNLNRQFLFRPHDVQKPKSMTAADAIKRMNPEVNVTAYELRVGAETEKVFSEDFFGKLDGV-ANALDNVDARIYMDRKCIFNRIPLVETGTLGTLGNVQVIV 563 HSP 2 Score: 63.5438 bits (153), Expect = 5.721e-10 Identity = 43/150 (28.67%), Postives = 78/150 (52.00%), Query Frame = -2
Query: 1545 YDRQIRLWGENGQIALENAKVCLIGSGCIGTEILKNLILPGIGSFTIVDDSVVTEEDLSNNFFVQKSDLNQSRAEVTARLLRELNEEVIVNYIEDSVESLMFRNPEYFLEFSVVIACGLNNKDLVDLDRLLYPENIGLIVCHSIGMVANL 1994
Y RQ+ + G + + N+ + L G G +G EI KN+IL G+ S T+ D + DLS+ F++ ++D+ ++RAE + L ELN NY+ +V E+ +F VV+ + ++ + + + I LI+ + G+ A +
Sbjct: 17 YSRQLYVLGHDAMRRMANSDILLSGLGGLGLEIAKNVILGGVKSITLHDTATCGLHDLSSQFYLTEADIGKNRAEASCAQLAELN-----NYVR-TVSHTGPLTEEFLRKFRVVVLTNSDGEEQQRIAKFAHENGIALIIAETRGLFAKV 160
BLAST of SMED30023745 vs. Ensembl Fly
Match: Uba1 (gene:FBgn0023143 transcript:FBtr0088499) HSP 1 Score: 64.6994 bits (156), Expect = 2.558e-10 Identity = 45/162 (27.78%), Postives = 86/162 (53.09%), Query Frame = -2
Query: 1533 RYDRQIRLWGENGQIALENAKVCLIGSGCIGTEILKNLILPGIGSFT----IVDDSVVTEEDLSNNFFVQKSDLNQSRAEVTARLLRELNEEVIVNYIEDSV--ESLMFRNPEYFLEFSVVIACGLNNKDL-VDLDRLLYPENIGLIVCHSIGMVANLRVII 1997
RYD QI ++G+ Q L ++K ++G+G IG E+LKN + G+G+ + D ++ + +L+ F + D+ + ++ A ++ +N EV V E V E+ + ++F + V A L+N D + +DR I L+ ++G + N++VI+
Sbjct: 586 RYDSQIAIFGKKFQEKLADSKWFIVGAGAIGCELLKNFGMLGLGTGNGQIFVTDMDLIEKSNLNRQFLFRPHDVQKPKSMTAADAIKRMNPEVNVTAYELRVGAETEKVFSEDFFGKLDGV-ANALDNVDARIYMDRKCIFNRIPLVETGTLGTLGNVQVIV 746 HSP 2 Score: 63.5438 bits (153), Expect = 5.711e-10 Identity = 43/150 (28.67%), Postives = 78/150 (52.00%), Query Frame = -2
Query: 1545 YDRQIRLWGENGQIALENAKVCLIGSGCIGTEILKNLILPGIGSFTIVDDSVVTEEDLSNNFFVQKSDLNQSRAEVTARLLRELNEEVIVNYIEDSVESLMFRNPEYFLEFSVVIACGLNNKDLVDLDRLLYPENIGLIVCHSIGMVANL 1994
Y RQ+ + G + + N+ + L G G +G EI KN+IL G+ S T+ D + DLS+ F++ ++D+ ++RAE + L ELN NY+ +V E+ +F VV+ + ++ + + + I LI+ + G+ A +
Sbjct: 200 YSRQLYVLGHDAMRRMANSDILLSGLGGLGLEIAKNVILGGVKSITLHDTATCGLHDLSSQFYLTEADIGKNRAEASCAQLAELN-----NYVR-TVSHTGPLTEEFLRKFRVVVLTNSDGEEQQRIAKFAHENGIALIIAETRGLFAKV 343
BLAST of SMED30023745 vs. Ensembl Zebrafish
Match: nae1 (nedd8 activating enzyme E1 subunit 1 [Source:NCBI gene;Acc:573336]) HSP 1 Score: 374.785 bits (961), Expect = 4.872e-122 Identity = 195/537 (36.31%), Postives = 320/537 (59.59%), Query Frame = -2
Query: 423 KTKRYDRQIRLWGENGQIALENAKVCLIGSGCIGTEILKNLILPGIGSFTIVDDSVVTEEDLSNNFFVQKSDLNQSRAEVTARLLRELNEEVIVNYIEDSVESLMFRNPEYFLEFSVVIACGLNNKDLVDLDRLLYPENIGLIVCHSIGMVANLRVIIQEHPVVEAKPDNIKPDLRLDKPLWEFVEFCDSQNLHEMSYKELAYCPWIIILFKYITS-FQSKFAKWPDSYKDKQILRKMINEGRXXXXXXXXXXXXXXXSQYLDSFEEAVKSIPTTVFLSEIPINVRTLLEDSRCIDINRESKSFWILVAALKDFIRSEGNGSLPLRGEIPDMTADTGRYVAIQKIYRQASEKDIIHLEKHVYRICQTFQIPVTDELNRNSIARFARNCQHIQILSSYSLERELKTSPTHTEELV-CPET--DELSWYLALRAAVSFYTVERRWPGQRQIDSEIDVPHLR----KLLQQ-DMNACVKFKDASVRDDFLLEMCRFSGVQLHSVCAFIAGIAAQEVIKLITGQYVPVMQPLMYNAIRQTT 2006
K ++YDRQ+RLWG++GQ ALENA VCLI + GTEILKNL+LPGIG+FTIVD V+ ED+ NNFF+ S + ++RA+ LL+ELN +V N++E+S + L+ + E+F FS+VIA L + L +L+ + +VC + G++ +R+I++EH VVE+ PDN+ DLRLD+P E +S +L M K+ ++ PWII++ +Y+ + ++ P +YK+K+ R+++ EG K E + ++FEEA+K++ T + ++I + + +C +I +S SFW++ ++DF+++EGNG+LP+RG IPDM AD+ +++ +Q +YR + +D + KHV + Q+ + ++ I F +N ++++ SL E + +E+ C ++ E+ YL LR+ FY R+PG E D+ L+ LLQ+ +N +V+DD++ E CR+ + H+V AF+ G AAQE IK+IT Q+VP +YNA+ QT+
Sbjct: 8 KEQKYDRQLRLWGDHGQEALENAHVCLINATASGTEILKNLVLPGIGAFTIVDGHKVSGEDVGNNFFLSSSAIGKNRAQAATELLQELNSDVSGNFVEESPDKLLDNDCEFFHRFSLVIAVQLPESTCLRLGAVLWEAGVPFLVCRTYGLIGYMRLIVKEHTVVESHPDNVLEDLRLDQPFTELKRHVESYDLDNMEKKDHSHTPWIIVVARYLEKWYNENNSQLPKNYKEKEAFRQLLREG--------ILKNENGGLEDEENFEEAIKNVNTALNPTKISSGTQDIFNAEQCENITSQSSSFWVMAHGVRDFVQNEGNGNLPVRGSIPDMIADSDKFIKLQNVYRDKAMRDAAVVSKHVEMLLQSVG-KTPESISEQEIKLFCKNAAFLRVVRCRSLADEYSVDTFNKDEITSCMDSPDSEMVLYLMLRSVDRFYQQHSRYPGVYNYQVEEDINKLKLCVNSLLQEYSLN-------VNVKDDYIHEFCRYGAAEPHTVAAFLGGSAAQEAIKIITHQFVPFNNTFLYNAMSQTS 528
BLAST of SMED30023745 vs. Ensembl Zebrafish
Match: nae1 (nedd8 activating enzyme E1 subunit 1 [Source:NCBI gene;Acc:573336]) HSP 1 Score: 374.785 bits (961), Expect = 4.872e-122 Identity = 195/537 (36.31%), Postives = 320/537 (59.59%), Query Frame = -2
Query: 423 KTKRYDRQIRLWGENGQIALENAKVCLIGSGCIGTEILKNLILPGIGSFTIVDDSVVTEEDLSNNFFVQKSDLNQSRAEVTARLLRELNEEVIVNYIEDSVESLMFRNPEYFLEFSVVIACGLNNKDLVDLDRLLYPENIGLIVCHSIGMVANLRVIIQEHPVVEAKPDNIKPDLRLDKPLWEFVEFCDSQNLHEMSYKELAYCPWIIILFKYITS-FQSKFAKWPDSYKDKQILRKMINEGRXXXXXXXXXXXXXXXSQYLDSFEEAVKSIPTTVFLSEIPINVRTLLEDSRCIDINRESKSFWILVAALKDFIRSEGNGSLPLRGEIPDMTADTGRYVAIQKIYRQASEKDIIHLEKHVYRICQTFQIPVTDELNRNSIARFARNCQHIQILSSYSLERELKTSPTHTEELV-CPET--DELSWYLALRAAVSFYTVERRWPGQRQIDSEIDVPHLR----KLLQQ-DMNACVKFKDASVRDDFLLEMCRFSGVQLHSVCAFIAGIAAQEVIKLITGQYVPVMQPLMYNAIRQTT 2006
K ++YDRQ+RLWG++GQ ALENA VCLI + GTEILKNL+LPGIG+FTIVD V+ ED+ NNFF+ S + ++RA+ LL+ELN +V N++E+S + L+ + E+F FS+VIA L + L +L+ + +VC + G++ +R+I++EH VVE+ PDN+ DLRLD+P E +S +L M K+ ++ PWII++ +Y+ + ++ P +YK+K+ R+++ EG K E + ++FEEA+K++ T + ++I + + +C +I +S SFW++ ++DF+++EGNG+LP+RG IPDM AD+ +++ +Q +YR + +D + KHV + Q+ + ++ I F +N ++++ SL E + +E+ C ++ E+ YL LR+ FY R+PG E D+ L+ LLQ+ +N +V+DD++ E CR+ + H+V AF+ G AAQE IK+IT Q+VP +YNA+ QT+
Sbjct: 8 KEQKYDRQLRLWGDHGQEALENAHVCLINATASGTEILKNLVLPGIGAFTIVDGHKVSGEDVGNNFFLSSSAIGKNRAQAATELLQELNSDVSGNFVEESPDKLLDNDCEFFHRFSLVIAVQLPESTCLRLGAVLWEAGVPFLVCRTYGLIGYMRLIVKEHTVVESHPDNVLEDLRLDQPFTELKRHVESYDLDNMEKKDHSHTPWIIVVARYLEKWYNENNSQLPKNYKEKEAFRQLLREG--------ILKNENGGLEDEENFEEAIKNVNTALNPTKISSGTQDIFNAEQCENITSQSSSFWVMAHGVRDFVQNEGNGNLPVRGSIPDMIADSDKFIKLQNVYRDKAMRDAAVVSKHVEMLLQSVG-KTPESISEQEIKLFCKNAAFLRVVRCRSLADEYSVDTFNKDEITSCMDSPDSEMVLYLMLRSVDRFYQQHSRYPGVYNYQVEEDINKLKLCVNSLLQEYSLN-------VNVKDDYIHEFCRYGAAEPHTVAAFLGGSAAQEAIKIITHQFVPFNNTFLYNAMSQTS 528
BLAST of SMED30023745 vs. Ensembl Zebrafish
Match: nae1 (nedd8 activating enzyme E1 subunit 1 [Source:NCBI gene;Acc:573336]) HSP 1 Score: 357.836 bits (917), Expect = 3.044e-115 Identity = 194/552 (35.14%), Postives = 317/552 (57.43%), Query Frame = -2
Query: 423 KTKRYDRQIRLWGENGQIALENAKVCLIGSGCIGTEILKNLILPGIGSFTIVDDSVVTEEDLSNNFFVQKSD---------------LNQSRAEVTARLLRELNEEVIVNYIEDSVESLMFRNPEYFLEFSVVIACGLNNKDLVDLDRLLYPENIGLIVCHSIGMVANLRVIIQEHPVVEAKPDNIKPDLRLDKPLWEFVEFCDSQNLHEMSYKELAYCPWIIILFKYITS-FQSKFAKWPDSYKDKQILRKMINEGRXXXXXXXXXXXXXXXSQYLDSFEEAVKSIPTTVFLSEIPINVRTLLEDSRCIDINRESKSFWILVAALKDFIRSEGNGSLPLRGEIPDMTADTGRYVAIQKIYRQASEKDIIHLEKHVYRICQTFQIPVTDELNRNSIARFARNCQHIQILSSYSLERELKTSPTHTEELV-CPET--DELSWYLALRAAVSFYTVERRWPGQRQIDSEIDVPHLR----KLLQQ-DMNACVKFKDASVRDDFLLEMCRFSGVQLHSVCAFIAGIAAQEVIKLITGQYVPVMQPLMYNAIRQTT 2006
K ++YDRQ+RLWG++GQ ALENA VCLI + GTEILKNL+LPGIG+FTIVD V+ ED+ NN K+ Q+RA+ LL+ELN +V N++E+S + L+ + E+F FS+VIA L + L +L+ + +VC + G++ +R+I++EH VVE+ PDN+ DLRLD+P E +S +L M K+ ++ PWII++ +Y+ + ++ P +YK+K+ R+++ EG K E + ++FEEA+K++ T + ++I + + +C +I +S SFW++ ++DF+++EGNG+LP+RG IPDM AD+ +++ +Q +YR + +D + KHV + Q+ + ++ I F +N ++++ SL E + +E+ C ++ E+ YL LR+ FY R+PG E D+ L+ LLQ+ +N +V+DD++ E CR+ + H+V AF+ G AAQE IK+IT Q+VP +YNA+ QT+
Sbjct: 8 KEQKYDRQLRLWGDHGQEALENAHVCLINATASGTEILKNLVLPGIGAFTIVDGHKVSGEDVGNNIVSIKTSNYPTLMWHMVIDTGLFFQNRAQAATELLQELNSDVSGNFVEESPDKLLDNDCEFFHRFSLVIAVQLPESTCLRLGAVLWEAGVPFLVCRTYGLIGYMRLIVKEHTVVESHPDNVLEDLRLDQPFTELKRHVESYDLDNMEKKDHSHTPWIIVVARYLEKWYNENNSQLPKNYKEKEAFRQLLREG--------ILKNENGGLEDEENFEEAIKNVNTALNPTKISSGTQDIFNAEQCENITSQSSSFWVMAHGVRDFVQNEGNGNLPVRGSIPDMIADSDKFIKLQNVYRDKAMRDAAVVSKHVEMLLQSVG-KTPESISEQEIKLFCKNAAFLRVVRCRSLADEYSVDTFNKDEITSCMDSPDSEMVLYLMLRSVDRFYQQHSRYPGVYNYQVEEDINKLKLCVNSLLQEYSLN-------VNVKDDYIHEFCRYGAAEPHTVAAFLGGSAAQEAIKIITHQFVPFNNTFLYNAMSQTS 543
BLAST of SMED30023745 vs. Ensembl Zebrafish
Match: sae1 (SUMO1 activating enzyme subunit 1 [Source:NCBI gene;Acc:415148]) HSP 1 Score: 82.4185 bits (202), Expect = 1.611e-16 Identity = 57/177 (32.20%), Postives = 87/177 (49.15%), Query Frame = -2
Query: 1491 EKTKRYDRQIRLWGENGQIALENAKVCLIGSGCIGTEILKNLILPGIGSFTIVDDSVVTEEDLSNNFFVQKSDLNQSRAEVTARLLRELNEEVIVNYIEDSVESLMFRNPEYFLEFSVVIACGLNNKDLVDLDRLLYPENIGLIVCHSIG----MVANLRVIIQEHPVVEAKPDNIK 2009
E+ +YDRQIRLWG + Q L ++V L+G +G E+ KNLIL G+ T++D VTEE F + Q+ A+ + + LN V V + VES + ++F +F V + +V +D+L NI + G M ++L QE+ VE KP +K
Sbjct: 15 EEAAQYDRQIRLWGLDAQKRLRGSRVLLVGLRGLGAEVAKNLILAGVKGLTLLDHEQVTEESRRAQFLIPVDADGQNHAQASLERAQFLNPMVEVKADTEPVES---KPDDFFFQFDAVCLTRCSRDLMVRVDQLCASRNIKVFCGDVYGYNGYMFSDLG---QEYHYVEEKPKVVK 185
BLAST of SMED30023745 vs. Ensembl Zebrafish
Match: uba1 (ubiquitin-like modifier activating enzyme 1 [Source:ZFIN;Acc:ZDB-GENE-040426-2009]) HSP 1 Score: 68.9366 bits (167), Expect = 6.184e-13 Identity = 47/149 (31.54%), Postives = 79/149 (53.02%), Query Frame = -2
Query: 1557 YDRQIRLWGENGQIALENAKVCLIGSGCIGTEILKNLILPGIGSFTIVDDSVVTEEDLSNNFFVQKSDLNQSRAEVTARLLRELNEEVIVNYIEDSVESLMFRNPEYFLEFSVVIACGLNNKDLVDLDRL---LYPENIGLIVCHSIGM 1994
Y RQ+ + G + ++++ V + G +G EI KN+IL G+ S T+ D V +DLS+ F++++ DL ++RA+V+ L ELN V V ++ + EY +F VV+ L N L + R+ + I LIV + G+
Sbjct: 55 YSRQLYVLGHDAMKRMQSSNVLISGLRGLGVEIAKNVILGGVKSVTLHDQGVAEWKDLSSQFYLREEDLGKNRADVSQPRLAELNSYVPVTSYTGTLTN------EYLTKFQVVV---LTNSSLDEQTRIGEFCHSNGIKLIVADTRGL 194
BLAST of SMED30023745 vs. Ensembl Xenopus
Match: gng2 (guanine nucleotide binding protein (G protein), gamma 2 [Source:Xenbase;Acc:XB-GENE-961282]) HSP 1 Score: 390.963 bits (1003), Expect = 4.181e-128 Identity = 205/534 (38.39%), Postives = 328/534 (61.42%), Query Frame = -2
Query: 423 KTKRYDRQIRLWGENGQIALENAKVCLIGSGCIGTEILKNLILPGIGSFTIVDDSVVTEEDLSNNFFVQKSDLNQSRAEVTARLLRELNEEVIVNYIEDSVESLMFRNPEYFLEFSVVIACGLNNKDLVDLDRLLYPENIGLIVCHSIGMVANLRVIIQEHPVVEAKPDNIKPDLRLDKPLWEFVEFCDSQNLHEMSYKELAYCPWIIILFKYITSFQSK-FAKWPDSYKDKQILRKMINEGRXXXXXXXXXXXXXXXSQYLDSFEEAVKSIPTTVFLSEIPINVRTLLEDSRCIDINRESKSFWILVAALKDFIRSEGNGSLPLRGEIPDMTADTGRYVAIQKIYRQASEKDIIHLEKHVYRICQTFQIPVTDELNRNSIARFARNCQHIQILSSYSLERELKTSPTHTEELVC---PETDELSWYLALRAAVSFYTVERRWPGQRQIDSEIDVPHLRKLLQQDMNACVKFKDAS--VRDDFLLEMCRFSGVQLHSVCAFIAGIAAQEVIKLITGQYVPVMQPLMYNAIRQTT 2006
K ++YDRQ+RLWG++GQ ALE+A VCLI + GTEILKNL+LPGIGSFTIVD +VVT ED+ NNFF+ + + ++RA+ + LL+ELN++V N++ +S E L+ ++P +F +F++VI L L+ L L+ NI L+VC + G V +R++++EH V+E+ PDN DLRL +P E E S +L M K+ ++ PWII++ K++ ++S+ + P SYK+K+ R +I +G K E + + ++FEEA+K++ T + ++++P +V +L D RC ++ +S SFWIL A+K+F+ EG G+LPLRG IPDM AD+ +++ +Q IYR+ ++KD +E V ++ Q+ P + ++ I F RNC ++++ SLE E +++V + +E+ YL LRA F R+PG E D+ L+ L N ++ S V+D+++ E CR+ + H+V +F+ G AAQE IK+IT Q+V +YNA+ QT+
Sbjct: 8 KEQKYDRQLRLWGDHGQEALESAHVCLINATATGTEILKNLVLPGIGSFTIVDGNVVTGEDVGNNFFLTRESIGKNRAQTSMELLQELNDDVTGNFVPESPEQLLEKDPSFFCKFTIVITTQLPESTLLRLAETLWDSNIPLLVCRTYGFVGYMRIVVKEHTVIESHPDNALEDLRLGQPFPELREHLQSYDLDHMERKDHSHTPWIIVVAKFLDKWRSENGGQMPKSYKEKESFRDLIRQG--------ILKNENGVPEDEENFEEAIKNVNTALNITKVPSSVEEILNDDRCTNLTHQSTSFWILARAVKEFMTVEGKGNLPLRGTIPDMIADSDKFIKLQNIYREKAKKDASAVESCVSKLLQSVGRP-PESISEREIRLFCRNCAFLRVVRCRSLEEEYGLDTAKKDDIVSLMENQDNEIVLYLMLRAVDRFQKQLGRYPGIYNYQIEGDIGKLKSCL----NGLLQEYGLSLTVKDEYIQEFCRYGAAEPHTVASFLGGAAAQEAIKIITKQFVIFNNTFIYNAMLQTS 528
BLAST of SMED30023745 vs. Ensembl Xenopus
Match: gng2 (guanine nucleotide binding protein (G protein), gamma 2 [Source:Xenbase;Acc:XB-GENE-961282]) HSP 1 Score: 325.094 bits (832), Expect = 3.547e-103 Identity = 191/532 (35.90%), Postives = 301/532 (56.58%), Query Frame = -2
Query: 423 KTKRYDRQIRLWGENGQIALENAKVCLIGSGCIGTEILKNLILPGIGSFTIVDDSVVTEEDLSNNFFVQKSDLNQSRAEVTARLLRELNEEVIVNYIEDSVESLMFRNPEYFLEFSVVIACGLNNKDLVDLDRLLYPENIGLIVCHSIGMVANLRVIIQEHPVVEAKPDNIKPDLRLDKPLWEFVEFCDSQNLHEMSYKELAYCPWIIILFKYITSFQSK-FAKWPDSYKDKQILRKMINEGRXXXXXXXXXXXXXXXSQYLDSFEEAVKSIPTTVFLSEIPINVRTLLEDSRCIDINRESKSFWILVAALKDFIRSEGNGSLPLRGEIPDMTADTGRYVAIQKIYRQASEKDIIHLEKHVYRICQTFQIPVTDELNRNSIARFARNCQHIQILSSYSLERELKTSPTHTEELVC---PETDELSWYLALRAAVSFYTVERRWPGQRQIDSEIDVPHLRKLLQQDMNACVKFKDASVRDDFLLEMCRFSGVQLHSVCAFIAGIAAQEVIKLITGQYVPVMQPLMYNAIRQTT 2006
K ++YDRQ+RLWG++GQ ALE+A VCLI + GTEILKNL+LPGIGSFTIVD +VVT ED +RA+ + LL+ELN++V N++ +S E L+ ++P +F +F++VI L L+ L L+ NI L+VC + G V +R++++EH V+E+ PDN DLRL +P E E S +L M K+ ++ PWII++ K++ ++S+ + P SYK+K+ R +I +G K E + + ++FEEA+K++ T + ++++P + L S S SFWIL A+K+F+ EG G+LPLRG IPDM AD+ +++ +Q IYR+ ++KD +E V ++ Q+ P + ++ I F +NC ++++ SLE E +++V + +E+ YL LRA F R+PG E D+ L+ L + L E ++++ FI+G AAQE IK+IT Q+V +YNA+ QT+
Sbjct: 8 KEQKYDRQLRLWGDHGQEALESAHVCLINATATGTEILKNLVLPGIGSFTIVDGNVVTGED--------------NRAQTSMELLQELNDDVTGNFVPESPEQLLEKDPSFFCKFTIVITTQLPESTLLRLAETLWDSNIPLLVCRTYGFVGYMRIVVKEHTVIESHPDNALEDLRLGQPFPELREHLQSYDLDHMERKDHSHTPWIIVVAKFLDKWRSENGGQMPKSYKEKESFRDLIRQG--------ILKNENGVPEDEENFEEAIKNVNTALNITKVPSSSFLYLFYS-------PSTSFWILARAVKEFMTVEGKGNLPLRGTIPDMIADSDKFIKLQNIYREKAKKDASAVESCVSKLLQSVGRP-PESISEREIRLFCKNCAFLRVVRCRSLEEEYGLDTAKKDDIVSLMENQDNEIVLYLMLRAVDRFQKQLGRYPGIYNYQIEGDIGKLKSCL----------------NGLLQEYVLVQAAVINNLFCFISGAAAQEAIKIITKQFVIFNNTFIYNAMLQTS 493
BLAST of SMED30023745 vs. Ensembl Xenopus
Match: gng2 (guanine nucleotide binding protein (G protein), gamma 2 [Source:Xenbase;Acc:XB-GENE-961282]) HSP 1 Score: 323.553 bits (828), Expect = 1.258e-102 Identity = 187/533 (35.08%), Postives = 296/533 (55.53%), Query Frame = -2
Query: 423 KTKRYDRQIRLWGENGQIALENAKVCLIGSGCIGTEILKNLILPGIGSFTIVDDSVVTEEDLSNN-FFVQKSDLNQSRAEVTARLLRELNEEVIVNYIEDSVESLMFRNPEYFLEFSVVIACGLNNKDLVDLDRLLYPENIGLIVCHSIGMVANLRVIIQEHPVVEAKPDNIKPDLRLDKPLWEFVEFCDSQNLHEMSYKELAYCPWIIILFKYITSFQSK-FAKWPDSYKDKQILRKMINEGRXXXXXXXXXXXXXXXSQYLDSFEEAVKSIPTTVFLSEIPINVRTLLEDSRCIDINRESKSFWILVAALKDFIRSEGNGSLPLRGEIPDMTADTGRYVAIQKIYRQASEKDIIHLEKHVYRICQTFQIPVTDELNRNSIARFARNCQHIQILSSYSLERELKTSPTHTEELVC---PETDELSWYLALRAAVSFYTVERRWPGQRQIDSEIDVPHLRKLLQQDMNACVKFKDASVRDDFLLEMCRFSGVQLHSVCAFIAGIAAQEVIKLITGQYVPVMQPLMYNAIRQTT 2006
K ++YDRQ+RLWG++GQ ALE+A VCLI + GTEILKNL+LPGIGSFTIVD +VVT ED ++ +F+Q S E L+ ++P +F +F++VI L L+ L L+ NI L+VC + G V +R++++EH V+E+ PDN DLRL +P E E S +L M K+ ++ PWII++ K++ ++S+ + P SYK+K+ R +I +G K E + + ++FEEA+K++ T + ++++P +V +L D RC ++ +S SFWIL A+K+F+ EG G+LPLRG IPDM AD+ +++ +Q IYR+ ++KD +E V ++ Q+ P + ++ I F +NC ++++ SLE E +++V + +E+ YL LRA F R+PG E D+ L+ L + L E ++++ FI+G AAQE IK+IT Q+V +YNA+ QT+
Sbjct: 8 KEQKYDRQLRLWGDHGQEALESAHVCLINATATGTEILKNLVLPGIGSFTIVDGNVVTGEDFDHSVYFLQ------------------------------SPEQLLEKDPSFFCKFTIVITTQLPESTLLRLAETLWDSNIPLLVCRTYGFVGYMRIVVKEHTVIESHPDNALEDLRLGQPFPELREHLQSYDLDHMERKDHSHTPWIIVVAKFLDKWRSENGGQMPKSYKEKESFRDLIRQG--------ILKNENGVPEDEENFEEAIKNVNTALNITKVPSSVEEILNDDRCTNLTHQSTSFWILARAVKEFMTVEGKGNLPLRGTIPDMIADSDKFIKLQNIYREKAKKDASAVESCVSKLLQSVGRP-PESISEREIRLFCKNCAFLRVVRCRSLEEEYGLDTAKKDDIVSLMENQDNEIVLYLMLRAVDRFQKQLGRYPGIYNYQIEGDIGKLKSCL----------------NGLLQEYVLVQAAVINNLFCFISGAAAQEAIKIITKQFVIFNNTFIYNAMLQTS 485
BLAST of SMED30023745 vs. Ensembl Xenopus
Match: gng2 (guanine nucleotide binding protein (G protein), gamma 2 [Source:Xenbase;Acc:XB-GENE-961282]) HSP 1 Score: 322.783 bits (826), Expect = 2.370e-102 Identity = 188/532 (35.34%), Postives = 295/532 (55.45%), Query Frame = -2
Query: 423 KTKRYDRQIRLWGENGQIALENAKVCLIGSGCIGTEILKNLILPGIGSFTIVDDSVVTEEDLSNNFFVQKSDLNQSRAEVTARLLRELNEEVIVNYIEDSVESLMFRNPEYFLEFSVVIACGLNNKDLVDLDRLLYPENIGLIVCHSIGMVANLRVIIQEHPVVEAKPDNIKPDLRLDKPLWEFVEFCDSQNLHEMSYKELAYCPWIIILFKYITSFQSK-FAKWPDSYKDKQILRKMINEGRXXXXXXXXXXXXXXXSQYLDSFEEAVKSIPTTVFLSEIPINVRTLLEDSRCIDINRESKSFWILVAALKDFIRSEGNGSLPLRGEIPDMTADTGRYVAIQKIYRQASEKDIIHLEKHVYRICQTFQIPVTDELNRNSIARFARNCQHIQILSSYSLERELKTSPTHTEELVC---PETDELSWYLALRAAVSFYTVERRWPGQRQIDSEIDVPHLRKLLQQDMNACVKFKDASVRDDFLLEMCRFSGVQLHSVCAFIAGIAAQEVIKLITGQYVPVMQPLMYNAIRQTT 2006
K ++YDRQ+RLWG++GQ ALE+A VCLI + GTEILKNL+LPGIGSFTIVD +VVT ED+ N KS L+ E L+ ++P +F +F++VI L L+ L L+ NI L+VC + G V +R++++EH V+E+ PDN DLRL +P E E S +L M K+ ++ PWII++ K++ ++S+ + P SYK+K+ R +I +G K E + + ++FEEA+K++ T + ++++P +V +L D RC ++ +S SFWIL A+K+F+ EG G+LPLRG IPDM AD+ +++ +Q IYR+ ++KD +E V ++ Q+ P + ++ I F +NC ++++ SLE E +++V + +E+ YL LRA F R+PG E D+ L+ L + L E ++++ FI+G AAQE IK+IT Q+V +YNA+ QT+
Sbjct: 8 KEQKYDRQLRLWGDHGQEALESAHVCLINATATGTEILKNLVLPGIGSFTIVDGNVVTGEDVGN-----KSALHP--------------------------EQLLEKDPSFFCKFTIVITTQLPESTLLRLAETLWDSNIPLLVCRTYGFVGYMRIVVKEHTVIESHPDNALEDLRLGQPFPELREHLQSYDLDHMERKDHSHTPWIIVVAKFLDKWRSENGGQMPKSYKEKESFRDLIRQG--------ILKNENGVPEDEENFEEAIKNVNTALNITKVPSSVEEILNDDRCTNLTHQSTSFWILARAVKEFMTVEGKGNLPLRGTIPDMIADSDKFIKLQNIYREKAKKDASAVESCVSKLLQSVGRP-PESISEREIRLFCKNCAFLRVVRCRSLEEEYGLDTAKKDDIVSLMENQDNEIVLYLMLRAVDRFQKQLGRYPGIYNYQIEGDIGKLKSCL----------------NGLLQEYVLVQAAVINNLFCFISGAAAQEAIKIITKQFVIFNNTFIYNAMLQTS 483
BLAST of SMED30023745 vs. Ensembl Xenopus
Match: gng2 (guanine nucleotide binding protein (G protein), gamma 2 [Source:Xenbase;Acc:XB-GENE-961282]) HSP 1 Score: 310.842 bits (795), Expect = 1.020e-97 Identity = 179/525 (34.10%), Postives = 291/525 (55.43%), Query Frame = -2
Query: 423 RLWGENGQIALENAKVCLIGSGCIGTEILKNLILPGIGSFTIVDDSVVTEEDLSNNFFVQKSDLNQSRAEVTARLLRELNEEVIVNYIEDSVESLMFRNPEYFLEFSVVIACGLNNKDLVDLDRLLYPENIGLIVCHSIGMVANLRVIIQEHPVVEAKPDNIKPDLRLDKPLWEFVEFCDSQNLHEMSYKELAYCPWIIILFKYITSFQSK-FAKWPDSYKDKQILRKMINEGRXXXXXXXXXXXXXXXSQYLDSFEEAVKSIPTTVFLSEIPINVRTLLEDSRCIDINRESKSFWILVAALKDFIRSEGNGSLPLRGEIPDMTADTGRYVAIQKIYRQASEKDIIHLEKHVYRICQTFQIPVTDELNRNSIARFARNCQHIQILSSYSLERELKTSPTHTEELVC---PETDELSWYLALRAAVSFYTVERRWPGQRQIDSEIDVPHLRKLLQQDMNACVKFKDAS--VRDDFLLEMCRFSGVQLHSVCAFIAGIAAQEVIKLITGQYVPVMQPLMYNAIRQTT 1979
RLWG++GQ ALE+A VCLI + GTEILKNL +RA+ + LL+ELN++V N++ +S E L+ ++P +F +F++VI L L+ L L+ NI L+VC + G V +R++++EH V+E+ PDN DLRL +P E E S +L M K+ ++ PWII++ K++ ++S+ + P SYK+K+ R +I +G K E + + ++FEEA+K++ T + ++++P +V +L D RC ++ +S SFWIL A+K+F+ EG G+LPLRG IPDM AD+ +++ +Q IYR+ ++KD +E V ++ Q+ V + I+ RNC ++++ SLE E +++V + +E+ YL LRA F R+PG E D+ L+ L N ++ S V+D+++ E CR+ + H+V +F+ G AAQE IK+IT Q+V +YNA+ QT+
Sbjct: 14 RLWGDHGQEALESAHVCLINATATGTEILKNL----------------------------------NRAQTSMELLQELNDDVTGNFVPESPEQLLEKDPSFFCKFTIVITTQLPESTLLRLAETLWDSNIPLLVCRTYGFVGYMRIVVKEHTVIESHPDNALEDLRLGQPFPELREHLQSYDLDHMERKDHSHTPWIIVVAKFLDKWRSENGGQMPKSYKEKESFRDLIRQG--------ILKNENGVPEDEENFEEAIKNVNTALNITKVPSSVEEILNDDRCTNLTHQSTSFWILARAVKEFMTVEGKGNLPLRGTIPDMIADSDKFIKLQNIYREKAKKDASAVESCVSKLLQS----VGRVIFLTVISNSGRNCAFLRVVRCRSLEEEYGLDTAKKDDIVSLMENQDNEIVLYLMLRAVDRFQKQLGRYPGIYNYQIEGDIGKLKSCL----NGLLQEYGLSLTVKDEYIQEFCRYGAAEPHTVASFLGGAAAQEAIKIITKQFVIFNNTFIYNAMLQTS 488
BLAST of SMED30023745 vs. Ensembl Mouse
Match: Nae1 (NEDD8 activating enzyme E1 subunit 1 [Source:MGI Symbol;Acc:MGI:2384561]) HSP 1 Score: 391.734 bits (1005), Expect = 1.642e-128 Identity = 202/536 (37.69%), Postives = 323/536 (60.26%), Query Frame = -2
Query: 423 KTKRYDRQIRLWGENGQIALENAKVCLIGSGCIGTEILKNLILPGIGSFTIVDDSVVTEEDLSNNFFVQKSDLNQSRAEVTARLLRELNEEVIVNYIEDSVESLMFRNPEYFLEFSVVIACGLNNKDLVDLDRLLYPENIGLIVCHSIGMVANLRVIIQEHPVVEAKPDNIKPDLRLDKPLWEFVEFCDSQNLHEMSYKELAYCPWIIILFKYITS-FQSKFAKWPDSYKDKQILRKMINEGRXXXXXXXXXXXXXXXSQYLDSFEEAVKSIPTTVFLSEIPINVRTLLEDSRCIDINRESKSFWILVAALKDFIRSEGNGSLPLRGEIPDMTADTGRYVAIQKIYRQASEKDIIHLEKHVYRICQTF-QIPVTDELNRNSIARFARNCQHIQILSSYSLERELKTSPTHTEELVCP---ETDELSWYLALRAAVSFYTVERRWPGQRQIDSEIDVPHLRKLLQ---QDMNACVKFKDASVRDDFLLEMCRFSGVQLHSVCAFIAGIAAQEVIKLITGQYVPVMQPLMYNAIRQTT 2006
K ++YDRQ+RLWG++GQ ALE+A VCLI + GTEILKNL+LPGIGSFTI+D ++V+ ED NNFF+QKS + ++RA+ L+ELN +V +++E+S E+L+ +P +F F++V+A L L+ L +L+ I L++C + G+V +R+II+EHPV+E+ PDN DLRLDKP E E S +L M K+ ++ PWI+I+ KY+ + + P SYK+K+ R +I +G K E + ++FEEA+K++ T + ++IP ++ + D RCI+I +++ +FWIL ALK+F+ EG G+LP+RG IPDM AD+ +Y+ +Q +YR+ ++KD + HV ++ Q+ Q P + ++ + N ++++ SL E + +E++ +E+ YL LRA F+ R+PG E D+ L+ L Q+ V V+DD++ E CR+ + H++ AF+ G AAQEVIK+IT Q+V +Y+ + QT+
Sbjct: 9 KEQKYDRQLRLWGDHGQEALESAHVCLINATATGTEILKNLVLPGIGSFTIIDGNLVSGEDAGNNFFLQKSSIGKNRAQAAMEFLQELNSDVSGSFVEESPENLLDNDPSFFCRFTIVVATQLLESTLLRLADVLWNSQIPLLICRTYGLVGYMRIIIKEHPVIESHPDNALEDLRLDKPFPELREHLQSYDLDHMEKKDHSHTPWIVIIAKYLAQWYNETNGRIPKSYKEKEDFRDLIRQG--------ILKNENGAPEDEENFEEAIKNVNTALNTTQIPSSIEDIFNDDRCINITKQTPTFWILARALKEFVAKEGQGNLPVRGTIPDMIADSNKYIKLQNVYREKAKKDAAAVGNHVAKLLQSVGQAP--ESISEKELKLLCSNSAFLRVVRCRSLAEEYGLDTVNKDEIISSMDNPDNEIVLYLMLRAVDRFHKQHGRYPGVSNYQVEEDIGKLKSCLTGFLQEYGLSVM-----VKDDYVHEFCRYGAAEPHTIAAFLGGAAAQEVIKIITKQFVIFNNTYIYSGMSQTS 529
BLAST of SMED30023745 vs. Ensembl Mouse
Match: Nae1 (NEDD8 activating enzyme E1 subunit 1 [Source:MGI Symbol;Acc:MGI:2384561]) HSP 1 Score: 360.918 bits (925), Expect = 6.910e-117 Identity = 194/536 (36.19%), Postives = 307/536 (57.28%), Query Frame = -2
Query: 423 KTKRYDRQIRLWGENGQIALENAKVCLIGSGCIGTEILKNLILPGIGSFTIVDDSVVTEEDLSNNFFVQKSDLNQSRAEVTARLLRELNEEVIVNYIEDSVESLMFRNPEYFLEFSVVIACGLNNKDLVDLDRLLYPENIGLIVCHSIGMVANLRVIIQEHPVVEAKPDNIKPDLRLDKPLWEFVEFCDSQNLHEMSYKELAYCPWIIILFKYITS-FQSKFAKWPDSYKDKQILRKMINEGRXXXXXXXXXXXXXXXSQYLDSFEEAVKSIPTTVFLSEIPINVRTLLEDSRCIDINRESKSFWILVAALKDFIRSEGNGSLPLRGEIPDMTADTGRYVAIQKIYRQASEKDIIHLEKHVYRICQTF-QIPVTDELNRNSIARFARNCQHIQILSSYSLERELKTSPTHTEELVCP---ETDELSWYLALRAAVSFYTVERRWPGQRQIDSEIDVPHLRKLLQ---QDMNACVKFKDASVRDDFLLEMCRFSGVQLHSVCAFIAGIAAQEVIKLITGQYVPVMQPLMYNAIRQTT 2006
K ++YDRQ+RLWG++GQ ALE+A VCLI + GTEILKNL+LPGIGSFTI+D ++V+ ED NNFF+QKS + +S E+L+ +P +F F++V+A L L+ L +L+ I L++C + G+V +R+II+EHPV+E+ PDN DLRLDKP E E S +L M K+ ++ PWI+I+ KY+ + + P SYK+K+ R +I +G K E + ++FEEA+K++ T + ++IP ++ + D RCI+I +++ +FWIL ALK+F+ EG G+LP+RG IPDM AD+ +Y+ +Q +YR+ ++KD + HV ++ Q+ Q P + ++ + N ++++ SL E + +E++ +E+ YL LRA F+ R+PG E D+ L+ L Q+ V V+DD++ E CR+ + H++ AF+ G AAQEVIK+IT Q+V +Y+ + QT+
Sbjct: 9 KEQKYDRQLRLWGDHGQEALESAHVCLINATATGTEILKNLVLPGIGSFTIIDGNLVSGEDAGNNFFLQKSSIGKSP------------------------ENLLDNDPSFFCRFTIVVATQLLESTLLRLADVLWNSQIPLLICRTYGLVGYMRIIIKEHPVIESHPDNALEDLRLDKPFPELREHLQSYDLDHMEKKDHSHTPWIVIIAKYLAQWYNETNGRIPKSYKEKEDFRDLIRQG--------ILKNENGAPEDEENFEEAIKNVNTALNTTQIPSSIEDIFNDDRCINITKQTPTFWILARALKEFVAKEGQGNLPVRGTIPDMIADSNKYIKLQNVYREKAKKDAAAVGNHVAKLLQSVGQAP--ESISEKELKLLCSNSAFLRVVRCRSLAEEYGLDTVNKDEIISSMDNPDNEIVLYLMLRAVDRFHKQHGRYPGVSNYQVEEDIGKLKSCLTGFLQEYGLSVM-----VKDDYVHEFCRYGAAEPHTIAAFLGGAAAQEVIKIITKQFVIFNNTYIYSGMSQTS 505
BLAST of SMED30023745 vs. Ensembl Mouse
Match: Sae1 (SUMO1 activating enzyme subunit 1 [Source:MGI Symbol;Acc:MGI:1929264]) HSP 1 Score: 83.5741 bits (205), Expect = 7.052e-17 Identity = 59/204 (28.92%), Postives = 95/204 (46.57%), Query Frame = -2
Query: 1392 RYDRQIRLWGENGQIALENAKVCLIGSGCIGTEILKNLILPGIGSFTIVDDSVVTEEDLSNNFFVQKSDLNQSRAEVTARLLRELNEEVIVNYIEDSVESLMFRNPE-YFLEFSVVIACGLNNKDLVDLDRLLYPENIGLIVCHSIGMVANLRVIIQEHPVVEAKPDNIKPDLRL-DKPLWEFVEFCDSQNLHEMSYKELAYCP 1997
+YDRQIRLWG Q L ++V ++G +G EI KNLIL G+ T++D V+ ED F +Q + ++RAE + + LN V V + VE + PE +F +F V + ++ +D++ + +I G + EH VE K K + D P + + S+ M K++ +CP
Sbjct: 22 QYDRQIRLWGLEAQKRLRASRVLIVGMKGLGAEIAKNLILAGVKGLTMLDHEQVSPEDPGAQFLIQTGSVGRNRAEASLERAQNLNPMVDVKVDTEDVE----KKPESFFTKFDAVCLTCCSRDVIIKVDQICHRNSIKFFTGDVFGYHGYTFANLGEHEFVEEKTKVAKVSQGVEDGPEAKRAKLDSSETT--MVKKKVLFCP 219
BLAST of SMED30023745 vs. Ensembl Mouse
Match: Sae1 (SUMO1 activating enzyme subunit 1 [Source:MGI Symbol;Acc:MGI:1929264]) HSP 1 Score: 83.5741 bits (205), Expect = 7.370e-17 Identity = 59/204 (28.92%), Postives = 95/204 (46.57%), Query Frame = -2
Query: 1392 RYDRQIRLWGENGQIALENAKVCLIGSGCIGTEILKNLILPGIGSFTIVDDSVVTEEDLSNNFFVQKSDLNQSRAEVTARLLRELNEEVIVNYIEDSVESLMFRNPE-YFLEFSVVIACGLNNKDLVDLDRLLYPENIGLIVCHSIGMVANLRVIIQEHPVVEAKPDNIKPDLRL-DKPLWEFVEFCDSQNLHEMSYKELAYCP 1997
+YDRQIRLWG Q L ++V ++G +G EI KNLIL G+ T++D V+ ED F +Q + ++RAE + + LN V V + VE + PE +F +F V + ++ +D++ + +I G + EH VE K K + D P + + S+ M K++ +CP
Sbjct: 22 QYDRQIRLWGLEAQKRLRASRVLIVGMKGLGAEIAKNLILAGVKGLTMLDHEQVSPEDPGAQFLIQTGSVGRNRAEASLERAQNLNPMVDVKVDTEDVE----KKPESFFTKFDAVCLTCCSRDVIIKVDQICHRNSIKFFTGDVFGYHGYTFANLGEHEFVEEKTKVAKVSQGVEDGPEAKRAKLDSSETT--MVKKKVLFCP 219
BLAST of SMED30023745 vs. Ensembl Mouse
Match: Sae1 (SUMO1 activating enzyme subunit 1 [Source:MGI Symbol;Acc:MGI:1929264]) HSP 1 Score: 83.5741 bits (205), Expect = 7.370e-17 Identity = 59/204 (28.92%), Postives = 95/204 (46.57%), Query Frame = -2
Query: 1392 RYDRQIRLWGENGQIALENAKVCLIGSGCIGTEILKNLILPGIGSFTIVDDSVVTEEDLSNNFFVQKSDLNQSRAEVTARLLRELNEEVIVNYIEDSVESLMFRNPE-YFLEFSVVIACGLNNKDLVDLDRLLYPENIGLIVCHSIGMVANLRVIIQEHPVVEAKPDNIKPDLRL-DKPLWEFVEFCDSQNLHEMSYKELAYCP 1997
+YDRQIRLWG Q L ++V ++G +G EI KNLIL G+ T++D V+ ED F +Q + ++RAE + + LN V V + VE + PE +F +F V + ++ +D++ + +I G + EH VE K K + D P + + S+ M K++ +CP
Sbjct: 22 QYDRQIRLWGLEAQKRLRASRVLIVGMKGLGAEIAKNLILAGVKGLTMLDHEQVSPEDPGAQFLIQTGSVGRNRAEASLERAQNLNPMVDVKVDTEDVE----KKPESFFTKFDAVCLTCCSRDVIIKVDQICHRNSIKFFTGDVFGYHGYTFANLGEHEFVEEKTKVAKVSQGVEDGPEAKRAKLDSSETT--MVKKKVLFCP 219
BLAST of SMED30023745 vs. UniProt/SwissProt
Match: sp|Q5ZIE6|ULA1_CHICK (NEDD8-activating enzyme E1 regulatory subunit OS=Gallus gallus OX=9031 GN=NAE1 PE=2 SV=1) HSP 1 Score: 397.897 bits (1021), Expect = 4.717e-130 Identity = 208/536 (38.81%), Postives = 328/536 (61.19%), Query Frame = -2
Query: 423 KTKRYDRQIRLWGENGQIALENAKVCLIGSGCIGTEILKNLILPGIGSFTIVDDSVVTEEDLSNNFFVQKSDLNQSRAEVTARLLRELNEEVIVNYIEDSVESLMFRNPEYFLEFSVVIACGLNNKDLVDLDRLLYPENIGLIVCHSIGMVANLRVIIQEHPVVEAKPDNIKPDLRLDKPLWEFVEFCDSQNLHEMSYKELAYCPWIIILFKYITS-FQSKFAKWPDSYKDKQILRKMINEGRXXXXXXXXXXXXXXXSQYLDSFEEAVKSIPTTVFLSEIPINVRTLLEDSRCIDINRESKSFWILVAALKDFIRSEGNGSLPLRGEIPDMTADTGRYVAIQKIYRQASEKDIIHLEKHVYRICQTFQIPVTDELNRNSIARFARNCQHIQILSSYSLERELKTSPTHTEELVC----PETDELSWYLALRAAVSFYTVERRWPGQRQIDSEIDVPHLRKLLQ---QDMNACVKFKDASVRDDFLLEMCRFSGVQLHSVCAFIAGIAAQEVIKLITGQYVPVMQPLMYNAIRQTT 2006
K +RYDRQ+RLWG++GQ ALE+A VC+I + GTEILKNL+LPGIGSFTIVD + V+ ED+ NNFF+QKS + QSRA+ LL+ELN +V N++E+S E+L+ +P +F F++V+A L+ ++ L LL+ NI L++C + G+V +R+II+EHPVVE+ PDN DLRLDK E E S +L M K+ ++ PWI+I+ KY+T F K + P SYK+K+ R++I +G K E + ++FEEA+K++ T + ++IP + + D C+++ +S SFWILV A+K+F+ +EG G LP+RG IPDM AD+ +++ +Q +YR+ +++DI + H ++ Q+ + ++ + N ++++ SL E + + +E++ P++ E+ YL LRA FY R+PG E D+ L+ L Q+ V V+DD++ E CR+ + H++ AF+ G AAQE+IK+ITGQ+V +Y+ + QT+
Sbjct: 10 KEQRYDRQLRLWGDHGQEALESAHVCVINATATGTEILKNLVLPGIGSFTIVDGNRVSGEDVGNNFFLQKSHIGQSRAQSATELLQELNNDVSGNFVEESPETLLDNDPSFFNRFNLVVATQLSESTVLRLAELLWNSNIPLLICRTYGLVGYMRIIIKEHPVVESHPDNALEDLRLDKQFPELTEHIQSYDLDHMDKKDHSHTPWIVIVAKYLTKWFNEKSDQLPKSYKEKEAFRQLIRQG--------ILKNENGTPEDEENFEEAIKNVNTALNTTKIPRCIEEIFNDDCCVNLTEQSPSFWILVRAVKEFVANEGQGCLPVRGTIPDMIADSSKFIKLQNVYREKAKRDIAAVGNHAAKLLQSLG-KAPESISERELKLLCSNSAFLRVVRCRSLSEEYGLNTFNKDEIISNMDNPDS-EVVLYLMLRAVDRFYKQHGRYPGVYNYQVEDDIGKLKSCLTSFLQEHGLSVL-----VKDDYVHEFCRYGAAEPHAIAAFMGGAAAQEIIKVITGQFVIFNNTYIYSGMSQTS 530
BLAST of SMED30023745 vs. UniProt/SwissProt
Match: sp|Q13564|ULA1_HUMAN (NEDD8-activating enzyme E1 regulatory subunit OS=Homo sapiens OX=9606 GN=NAE1 PE=1 SV=1) HSP 1 Score: 393.66 bits (1010), Expect = 1.789e-128 Identity = 207/540 (38.33%), Postives = 328/540 (60.74%), Query Frame = -2
Query: 423 KLLEKTKRYDRQIRLWGENGQIALENAKVCLIGSGCIGTEILKNLILPGIGSFTIVDDSVVTEEDLSNNFFVQKSDLNQSRAEVTARLLRELNEEVIVNYIEDSVESLMFRNPEYFLEFSVVIACGLNNKDLVDLDRLLYPENIGLIVCHSIGMVANLRVIIQEHPVVEAKPDNIKPDLRLDKPLWEFVEFCDSQNLHEMSYKELAYCPWIIILFKYITSFQSKF-AKWPDSYKDKQILRKMINEGRXXXXXXXXXXXXXXXSQYLDSFEEAVKSIPTTVFLSEIPINVRTLLEDSRCIDINRESKSFWILVAALKDFIRSEGNGSLPLRGEIPDMTADTGRYVAIQKIYRQASEKDIIHLEKHVYRICQTF-QIPVTDELNRNSIARFARNCQHIQILSSYSLERELKTSPTHTEELVCP---ETDELSWYLALRAAVSFYTVERRWPGQRQIDSEIDVPHLRKLLQ---QDMNACVKFKDASVRDDFLLEMCRFSGVQLHSVCAFIAGIAAQEVIKLITGQYVPVMQPLMYNAIRQTT 2018
KLL K ++YDRQ+RLWG++GQ ALE+A VCLI + GTEILKNL+LPGIGSFTI+D + V+ ED NNFF+Q+S + ++RAE L+ELN +V +++E+S E+L+ +P +F F+VV+A L + L +L+ I L++C + G+V +R+II+EHPV+E+ PDN DLRLDKP E E S +L M K+ ++ PWI+I+ KY+ + S+ + P +YK+K+ R +I +G K E + ++FEEA+K++ T + ++IP ++ + D RCI+I +++ SFWIL ALK+F+ EG G+LP+RG IPDM AD+G+Y+ +Q +YR+ ++KD + HV ++ Q+ Q P + ++ + N ++++ SL E + +E++ +E+ YL LRA F+ + R+PG E D+ L+ L Q+ V V+DD++ E CR+ + H++ AF+ G AAQEVIK+IT Q+V +Y+ + QT+
Sbjct: 6 KLL-KEQKYDRQLRLWGDHGQEALESAHVCLINATATGTEILKNLVLPGIGSFTIIDGNQVSGEDAGNNFFLQRSSIGKNRAEAAMEFLQELNSDVSGSFVEESPENLLDNDPSFFCRFTVVVATQLPESTSLRLADVLWNSQIPLLICRTYGLVGYMRIIIKEHPVIESHPDNALEDLRLDKPFPELREHFQSYDLDHMEKKDHSHTPWIVIIAKYLAQWYSETNGRIPKTYKEKEDFRDLIRQG--------ILKNENGAPEDEENFEEAIKNVNTALNTTQIPSSIEDIFNDDRCINITKQTPSFWILARALKEFVAKEGQGNLPVRGTIPDMIADSGKYIKLQNVYREKAKKDAAAVGNHVAKLLQSIGQAP--ESISEKELKLLCSNSAFLRVVRCRSLAEEYGLDTINKDEIISSMDNPDNEIVLYLMLRAVDRFHKQQGRYPGVSNYQVEEDIGKLKSCLTGFLQEYGLSVM-----VKDDYVHEFCRYGAAEPHTIAAFLGGAAAQEVIKIITKQFVIFNNTYIYSGMSQTS 529
BLAST of SMED30023745 vs. UniProt/SwissProt
Match: sp|Q8VBW6|ULA1_MOUSE (NEDD8-activating enzyme E1 regulatory subunit OS=Mus musculus OX=10090 GN=Nae1 PE=1 SV=1) HSP 1 Score: 391.734 bits (1005), Expect = 1.148e-127 Identity = 202/536 (37.69%), Postives = 323/536 (60.26%), Query Frame = -2
Query: 423 KTKRYDRQIRLWGENGQIALENAKVCLIGSGCIGTEILKNLILPGIGSFTIVDDSVVTEEDLSNNFFVQKSDLNQSRAEVTARLLRELNEEVIVNYIEDSVESLMFRNPEYFLEFSVVIACGLNNKDLVDLDRLLYPENIGLIVCHSIGMVANLRVIIQEHPVVEAKPDNIKPDLRLDKPLWEFVEFCDSQNLHEMSYKELAYCPWIIILFKYITS-FQSKFAKWPDSYKDKQILRKMINEGRXXXXXXXXXXXXXXXSQYLDSFEEAVKSIPTTVFLSEIPINVRTLLEDSRCIDINRESKSFWILVAALKDFIRSEGNGSLPLRGEIPDMTADTGRYVAIQKIYRQASEKDIIHLEKHVYRICQTF-QIPVTDELNRNSIARFARNCQHIQILSSYSLERELKTSPTHTEELVCP---ETDELSWYLALRAAVSFYTVERRWPGQRQIDSEIDVPHLRKLLQ---QDMNACVKFKDASVRDDFLLEMCRFSGVQLHSVCAFIAGIAAQEVIKLITGQYVPVMQPLMYNAIRQTT 2006
K ++YDRQ+RLWG++GQ ALE+A VCLI + GTEILKNL+LPGIGSFTI+D ++V+ ED NNFF+QKS + ++RA+ L+ELN +V +++E+S E+L+ +P +F F++V+A L L+ L +L+ I L++C + G+V +R+II+EHPV+E+ PDN DLRLDKP E E S +L M K+ ++ PWI+I+ KY+ + + P SYK+K+ R +I +G K E + ++FEEA+K++ T + ++IP ++ + D RCI+I +++ +FWIL ALK+F+ EG G+LP+RG IPDM AD+ +Y+ +Q +YR+ ++KD + HV ++ Q+ Q P + ++ + N ++++ SL E + +E++ +E+ YL LRA F+ R+PG E D+ L+ L Q+ V V+DD++ E CR+ + H++ AF+ G AAQEVIK+IT Q+V +Y+ + QT+
Sbjct: 9 KEQKYDRQLRLWGDHGQEALESAHVCLINATATGTEILKNLVLPGIGSFTIIDGNLVSGEDAGNNFFLQKSSIGKNRAQAAMEFLQELNSDVSGSFVEESPENLLDNDPSFFCRFTIVVATQLLESTLLRLADVLWNSQIPLLICRTYGLVGYMRIIIKEHPVIESHPDNALEDLRLDKPFPELREHLQSYDLDHMEKKDHSHTPWIVIIAKYLAQWYNETNGRIPKSYKEKEDFRDLIRQG--------ILKNENGAPEDEENFEEAIKNVNTALNTTQIPSSIEDIFNDDRCINITKQTPTFWILARALKEFVAKEGQGNLPVRGTIPDMIADSNKYIKLQNVYREKAKKDAAAVGNHVAKLLQSVGQAP--ESISEKELKLLCSNSAFLRVVRCRSLAEEYGLDTVNKDEIISSMDNPDNEIVLYLMLRAVDRFHKQHGRYPGVSNYQVEEDIGKLKSCLTGFLQEYGLSVM-----VKDDYVHEFCRYGAAEPHTIAAFLGGAAAQEVIKIITKQFVIFNNTYIYSGMSQTS 529
BLAST of SMED30023745 vs. UniProt/SwissProt
Match: sp|Q9Z1A5|ULA1_RAT (NEDD8-activating enzyme E1 regulatory subunit OS=Rattus norvegicus OX=10116 GN=Nae1 PE=1 SV=1) HSP 1 Score: 390.963 bits (1003), Expect = 2.490e-127 Identity = 204/536 (38.06%), Postives = 325/536 (60.63%), Query Frame = -2
Query: 423 KTKRYDRQIRLWGENGQIALENAKVCLIGSGCIGTEILKNLILPGIGSFTIVDDSVVTEEDLSNNFFVQKSDLNQSRAEVTARLLRELNEEVIVNYIEDSVESLMFRNPEYFLEFSVVIACGLNNKDLVDLDRLLYPENIGLIVCHSIGMVANLRVIIQEHPVVEAKPDNIKPDLRLDKPLWEFVEFCDSQNLHEMSYKELAYCPWIIILFKYITSFQSKF-AKWPDSYKDKQILRKMINEGRXXXXXXXXXXXXXXXSQYLDSFEEAVKSIPTTVFLSEIPINVRTLLEDSRCIDINRESKSFWILVAALKDFIRSEGNGSLPLRGEIPDMTADTGRYVAIQKIYRQASEKDIIHLEKHVYRICQT-FQIPVTDELNRNSIARFARNCQHIQILSSYSLERELKTSPTHTEELVCP---ETDELSWYLALRAAVSFYTVERRWPGQRQIDSEIDVPHLRKLLQ---QDMNACVKFKDASVRDDFLLEMCRFSGVQLHSVCAFIAGIAAQEVIKLITGQYVPVMQPLMYNAIRQTT 2006
K ++YDRQ+RLWG++GQ ALE+A VCLI + GTEILKNL+LPGIGSFTI+D + V+ ED+ NNFF+QK + ++RA+ L+ELN +V +++E+S E+L+ +P +F F++V+A L L+ L +L+ I L++C + G+V +R+II+EHPV+E+ PDN DLRLDKP E E S +L M K+ ++ PWI+I+ KY+ + S+ + P SYK+K+ R++I +G K E + ++FEEA+K++ T + ++IP ++ + D RCI+I +++ SFWIL ALK+F+ EG G+LP+RG IPDM AD+ +Y+ +Q +YR+ ++KD + HV ++ Q+ Q P + ++ + N ++++ SL E + +E++ +E+ YL LRA F+ R+PG E D+ L+ L Q+ V V+DD++ E CR+ + H+V AF+ G AAQEVIK+IT Q+V +Y+ + QT+
Sbjct: 9 KEQKYDRQLRLWGDHGQEALESAHVCLINATATGTEILKNLVLPGIGSFTIIDGNQVSGEDVGNNFFLQKCSIGKNRAQAAMEFLQELNSDVSGSFVEESPENLLDNDPSFFCRFTIVVATQLLESTLLRLADVLWNSQIPLLICRTYGLVGYMRIIIKEHPVIESHPDNALEDLRLDKPFPELREHFQSYDLDHMEKKDHSHTPWIVIIAKYLAQWYSETNGRIPKSYKEKEDFRELIRQG--------ILKNENGAPEDEENFEEAIKNVNTALNTTQIPSSIEDIFNDDRCINITKQTPSFWILARALKEFVAKEGQGNLPVRGTIPDMIADSNKYIKLQNVYREKAKKDAAAVGNHVAKLLQSCGQAP--ESISEKELKLLCSNSAFLRVVRCRSLAEEYGLHTVNKDEIISSMDNPDNEIVLYLMLRAVDRFHKQHGRYPGVSNYQVEEDIGKLKSCLTGFLQEYGLSVM-----VKDDYVHEFCRYGAAEPHTVAAFLGGAAAQEVIKIITKQFVIFNNTYIYSGMSQTS 529
BLAST of SMED30023745 vs. UniProt/SwissProt
Match: sp|Q6NTW6|ULA1_XENLA (NEDD8-activating enzyme E1 regulatory subunit OS=Xenopus laevis OX=8355 GN=nae1 PE=2 SV=1) HSP 1 Score: 389.423 bits (999), Expect = 7.872e-127 Identity = 207/535 (38.69%), Postives = 329/535 (61.50%), Query Frame = -2
Query: 423 KTKRYDRQIRLWGENGQIALENAKVCLIGSGCIGTEILKNLILPGIGSFTIVDDSVVTEEDLSNNFFVQKSDLNQSRAEVTARLLRELNEEVIVNYIEDSVESLMFRNPEYFLEFSVVIACGLNNKDLVDLDRLLYPENIGLIVCHSIGMVANLRVIIQEHPVVEAKPDNIKPDLRLDKPLWEFVEFCDSQNLHEMSYKELAYCPWIIILFKYITSFQSK-FAKWPDSYKDKQILRKMINEGRXXXXXXXXXXXXXXXSQYLDSFEEAVKSIPTTVFLSEIPINVRTLLEDSRCIDINRESKSFWILVAALKDFIRSEGNGSLPLRGEIPDMTADTGRYVAIQKIYRQASEKDIIHLEKHVYRICQTFQIPVTDELNRNSIARFARNCQHIQILSSYSLERELKTSPTHTEELVC----PETDELSWYLALRAAVSFYTVERRWPGQRQIDSEIDVPHLRKLLQQDMNACVKFKDAS--VRDDFLLEMCRFSGVQLHSVCAFIAGIAAQEVIKLITGQYVPVMQPLMYNAIRQTT 2006
K ++YDRQ+RLWG++GQ ALE+A VCLI + GTEILKNL+LPGIG FTIVD +VV ED+ NNFF+QK + ++RA+ + LL+ELN++V N++ +S E L+ ++P +F +F++VI+ L L+ L L+ NI L+VC + G V +R+I++EH V+E+ PDN DLRLD+P E E S +L M K+ ++ PWII++ KY+ ++S+ + P SYK+K+ R +I +G K E + + ++FEEA+K++ T + ++++ +V +L D RC ++ +S SFWIL A+K+F+ EG G+LPLRG IPDM AD+ +++ +Q IYR+ ++KD +E V ++ Q+ P + ++ I F RNC ++++ SLE E +++V P+ +E+ YL LRA F R+PG E D+ L+ L N ++ S V+D+++ E CR+ + H++ +F+ G AAQE IK+IT Q+V +YNA+ QT+
Sbjct: 8 KEQKYDRQLRLWGDHGQEALESAHVCLINATATGTEILKNLVLPGIGLFTIVDGNVVAGEDVGNNFFLQKESIGKNRAQTSMELLQELNDDVTGNFVPESPEQLLDKDPSFFCKFTMVISTQLPESTLLRLAETLWDSNIPLLVCRAYGFVGYMRIIVKEHTVIESHPDNALEDLRLDQPFPELREHLQSYDLDHMERKDHSHTPWIIVVAKYLDKWRSENGGQMPKSYKEKESFRDLIRQG--------ILKNENGVPEDEENFEEAIKNVNTALNITKVSSSVEEILNDDRCTNLTHQSTSFWILARAVKEFMAMEGKGNLPLRGTIPDMIADSDKFIKLQNIYREKAKKDASAVESCVSKLLQSVGRP-PESISERDIRLFCRNCAFLRVVRCRSLEEEYGLDTAKKDDIVSLMENPD-NEIVLYLMLRAVDRFQKQLGRYPGIYNYQIESDIGKLKSCL----NGLLQEYGLSLTVKDEYIQEFCRYGAAEPHTIASFLGGAAAQEAIKIITKQFVIFNNTFIYNAMLQTS 528
BLAST of SMED30023745 vs. TrEMBL
Match: A0A433TZ93 (NEDD8-activating enzyme E1 regulatory subunit OS=Elysia chlorotica OX=188477 GN=EGW08_005301 PE=3 SV=1) HSP 1 Score: 447.203 bits (1149), Expect = 1.138e-146 Identity = 228/542 (42.07%), Postives = 337/542 (62.18%), Query Frame = -2
Query: 423 LEKTKRYDRQIRLWGENGQIALENAKVCLIGSGCIGTEILKNLILPGIGSFTIVDDSVVTEEDLSNNFFVQKSDLNQSRAEVTARLLRELNEEVIVNYIEDSVESLMFRNPEYFLEFSVVIACGLNNKDLVDLDRLLYPENIGLIVCHSIGMVANLRVIIQEHPVVEAKPDNIKPDLRLDKPLWEFVEFCDSQNLHEMSYKELAYCPWIIILFKYITSFQSKF-AKWPDSYKDKQILRKMINEGRXXXXXXXXXXXXXXXSQYLDSFEEAVKSIPTTVFLSEIPINVRTLLEDSRCIDINRESKSFWILVAALKDFIRSEGNGSLPLRGEIPDMTADTGRYVAIQKIYRQASEKDIIHLEKHVYRICQTFQIPVTDELNRNSIARFARNCQHIQILSSYSLERELKTSPTHTEEL-----------VCPETDELSWYLALRAAVSFYTVERRWPGQRQIDSEIDVPHLRKLLQQDMNACVKFKDASVRDDFLLEMCRFSGVQLHSVCAFIAGIAAQEVIKLITGQYVPVMQPLMYNAIRQTT 2012
L+K +YDRQ+RLWG++GQ +LE A+VCLI + GTEILKNLILPGIGSFTI+D + V ED+ NNFF+ + +SRA+V LL ELNE+V +++E++V+ L+ +NP +F F+ VIA L+ + L+DL ++L+ NI L+VC G + LRV+I+EH ++E+ PDN DLRLD P ++C+S NL M K ++ PW++IL+KY+ ++ K K P +YK+K L+++I EG K E + + ++F+EA+KS+ +++ + IP ++R L +D C+ +N ESK+FWI+V ALK+F S+GNG LPLRG IPDMTAD+ RY+ +Q +YR +E D I + HV+ + I ++ I F RN + +L + SLE E +TEEL E D+ Y+ LRAA F+ R+PG E D+P L+ L + ++ V+DDF+ EMCR+ +LH+V AF+ G+AAQE IKLITGQ+VP+ +YN RQT+
Sbjct: 9 LDKNNKYDRQLRLWGDHGQSSLETARVCLINASATGTEILKNLILPGIGSFTILDGNKVQGEDVGNNFFLTADSIGKSRAQVATELLSELNEDVSGDFLEENVDDLLQKNPAFFTSFTTVIATHLSERTLLDLGKVLWTANIPLLVCRCYGFIGYLRVVIKEHTIIESHPDNFHEDLRLDYPFKGLKDYCNSLNLETMDKKTHSHTPWLVILYKYLQIWEEKHGGKMPKNYKEKVALKELIKEG--------IRKNEHGVPEEEENFDEAIKSVNSSLNATGIPSDIRALFDDPSCLKLNSESKNFWIMVRALKEFTESDGNGFLPLRGSIPDMTADSERYIQLQTVYRDQAELDSIAVAGHVHALLH--NIGRKGTISDAEIKTFCRNSAFLTVLRTRSLEEEYGADTANTEELGRHVMMEEEEDASDEGDDTVLYILLRAADRFFAEYTRYPGFFNNSVEADIPKLKTCLTKLIHDWG--LTTGVKDDFIQEMCRYGASELHTVSAFMGGVAAQEAIKLITGQFVPINNTFIYNGQRQTS 538
BLAST of SMED30023745 vs. TrEMBL
Match: A0A1S3I5G6 (NEDD8-activating enzyme E1 regulatory subunit OS=Lingula unguis OX=7574 GN=LOC106161146 PE=3 SV=1) HSP 1 Score: 427.172 bits (1097), Expect = 5.683e-139 Identity = 217/539 (40.26%), Postives = 337/539 (62.52%), Query Frame = -2
Query: 423 LEKTKRYDRQIRLWGENGQIALENAKVCLIGSGCIGTEILKNLILPGIGSFTIVDDSVVTEEDLSNNFFVQKSDLNQSRAEVTARLLRELNEEVIVNYIEDSVESLMFRNPEYFLEFSVVIACGLNNKDLVDLDRLLYPENIGLIVCHSIGMVANLRVIIQEHPVVEAKPDNIKPDLRLDKPLWEFVEFCDSQNLHEMSYKELAYCPWIIILFKYITSFQSKF-AKWPDSYKDKQILRKMINEGRXXXXXXXXXXXXXXXSQYLDSFEEAVKSIPTTVFLSEIPINVRTLLEDSRCIDINRESKSFWILVAALKDFIRSEGNGSLPLRGEIPDMTADTGRYVAIQKIYRQASEKDIIHLEKHVYRICQTFQIPVTDELNRNSIARFARNCQHIQILSSYSLERELKTSPTHTEELVC----PETDELSWYLALRAAVSFYTVERRWPGQRQIDSEIDVPHLR----KLLQQDMNACVKFKDASVRDDFLLEMCRFSGVQLHSVCAFIAGIAAQEVIKLITGQYVPVMQPLMYNAIRQTT 2012
L+KT++YDRQ+RLWG++GQ ALE+AKVCLI + GTEILKNLILPG+GSFTIVD VT ED+ NNFF+ K + +SRA+V LL ELNE+V N++E+S E L+ RNPE+F F++V+A L + L L L+ ++I L+V + G++ ++R++ +EH V+E+ PDN DLRLD+P ++CDS +L M K+ ++ PW++I+FKY+ ++ + P +YK+K +K+I +G + E + + ++F+EA+ ++ ++ + IP V+ + DS C+++ ESK FWIL A+KDF+ S G G+LPLRG IPDMTAD+ RY+ +Q++YRQ + + + V ++ Q+ D + + F RN +++L S+ +E + ELV + D+ WY+ LRA FY R+PG E D+P L+ KLL + N +++DDF+ EMCR+ ++HSV +F+ G+AAQE IK+ITGQ+VP+ +YNA++QT+
Sbjct: 8 LDKTRKYDRQLRLWGDHGQCALESAKVCLINATATGTEILKNLILPGVGSFTIVDGKKVTGEDVGNNFFLDKDKIGESRAQVATDLLLELNEDVTGNFMEESPELLLERNPEFFDTFTIVVAVDLPERVLHKLSGHLWEKDIPLMVVRAYGLIGSIRLVAREHTVIESHPDNAHEDLRLDRPFPGLTQYCDSLDLSTMKKKDHSHTPWLVIIFKYLQIWKEQHGGAAPKNYKEKNEFKKLIQQG--------VLRNEEGVPEVEENFDEAINNVNVSLVPTSIPSAVQNIFNDSECVNLQPESKPFWILARAVKDFVESVGEGALPLRGTIPDMTADSERYIQLQQVYRQQAAVEAEWVANRVQQLLQSLG-KSKDTITEADVKLFCRNASFLRVLQYRSIAQEYDPATAKIGELVGNIEESDEDDAIWYILLRAVDKFYADFNRYPGVYDDQVEADIPKLKTCLAKLLHEYGNVY------NIKDDFVHEMCRYGAAEIHSVASFLGGVAAQEAIKIITGQFVPMNNTYIYNAMKQTS 531
BLAST of SMED30023745 vs. TrEMBL
Match: A0A1S3I5C7 (NEDD8-activating enzyme E1 regulatory subunit OS=Lingula unguis OX=7574 GN=LOC106161146 PE=3 SV=1) HSP 1 Score: 422.935 bits (1086), Expect = 6.233e-137 Identity = 222/560 (39.64%), Postives = 343/560 (61.25%), Query Frame = -2
Query: 423 LEKTKRYDRQIRLWGENGQIALENAKVCLIGSGCIGTEILKNLILPGIGSFTIVDDSVVTEEDLSNNFFVQKSDLNQSRAEVTARLLRELNEEVIVNYIEDSVESLMFRNPEYFLEFSVVIACGLNNKDLVDLDRLLYPENIGLIVCHSIGMVANLRVIIQEHPVVEAKPDNIKPDLRLDKPLWEFVEFCDSQNLHEMSYKELAYCPWIIILFKYITSFQSKF-AKWPDSYKDKQILRKMINEGRXXXXXXXXXXXXXXXSQYLDSFEEAVKSIPTTVFLSEIPINVRTLLEDSRCIDINRESKSFWILVAALKDFIRSEGNGSLPLRGEIPDMTADTGRYVAIQKIYRQASEKDIIHLEKHVYRICQTFQI--PVTDEL-------NRNSIAR------------FARNCQHIQILSSYSLERELKTSPTHTEELVC----PETDELSWYLALRAAVSFYTVERRWPGQRQIDSEIDVPHLR----KLLQQDMNACVKFKDASVRDDFLLEMCRFSGVQLHSVCAFIAGIAAQEVIKLITGQYVPVMQPLMYNAIRQTT 2012
L+KT++YDRQ+RLWG++GQ ALE+AKVCLI + GTEILKNLILPG+GSFTIVD VT ED+ NNFF+ K + +SRA+V LL ELNE+V N++E+S E L+ RNPE+F F++V+A L + L L L+ ++I L+V + G++ ++R++ +EH V+E+ PDN DLRLD+P ++CDS +L M K+ ++ PW++I+FKY+ ++ + P +YK+K +K+I +G + E + + ++F+EA+ ++ ++ + IP V+ + DS C+++ ESK FWIL A+KDF+ S G G+LPLRG IPDMTAD+ RY+ +Q++YRQ + + + V ++ Q+ PV ++ N N+IA F RN +++L S+ +E + ELV + D+ WY+ LRA FY R+PG E D+P L+ KLL + N +++DDF+ EMCR+ ++HSV +F+ G+AAQE IK+ITGQ+VP+ +YNA++QT+
Sbjct: 8 LDKTRKYDRQLRLWGDHGQCALESAKVCLINATATGTEILKNLILPGVGSFTIVDGKKVTGEDVGNNFFLDKDKIGESRAQVATDLLLELNEDVTGNFMEESPELLLERNPEFFDTFTIVVAVDLPERVLHKLSGHLWEKDIPLMVVRAYGLIGSIRLVAREHTVIESHPDNAHEDLRLDRPFPGLTQYCDSLDLSTMKKKDHSHTPWLVIIFKYLQIWKEQHGGAAPKNYKEKNEFKKLIQQG--------VLRNEEGVPEVEENFDEAINNVNVSLVPTSIPSAVQNIFNDSECVNLQPESKPFWILARAVKDFVESVGEGALPLRGTIPDMTADSERYIQLQQVYRQQAAVEAEWVANRVQQLLQSLGKGEPVPTDMKSRCQSQNNNNIASSKDTITEADVKLFCRNASFLRVLQYRSIAQEYDPATAKIGELVGNIEESDEDDAIWYILLRAVDKFYADFNRYPGVYDDQVEADIPKLKTCLAKLLHEYGNVY------NIKDDFVHEMCRYGAAEIHSVASFLGGVAAQEAIKIITGQFVPMNNTYIYNAMKQTS 553
BLAST of SMED30023745 vs. TrEMBL
Match: R7TTJ5 (NEDD8-activating enzyme E1 regulatory subunit OS=Capitella teleta OX=283909 GN=CAPTEDRAFT_19394 PE=3 SV=1) HSP 1 Score: 421.009 bits (1081), Expect = 3.553e-136 Identity = 222/557 (39.86%), Postives = 340/557 (61.04%), Query Frame = -2
Query: 423 EKTKRYDRQIRLWGENGQIALENAKVCLIGSGCIGTEILKNLILPGIGSFTIVDDSVVTEEDLSNNFFVQKSDLNQSRAEVTARLLRELNEEVIVNYIEDSVESLMFRNPEYFLEFSVVIACGLNNKDLVDLDRLLYPENIGLIVCHSIGMVANLRVIIQEHPVVEAKPDNIKPDLRLDKPLWEFVEFCDSQNLHEMSYKELAYCPWIIILFKYITSFQ-SKFAKWPDSYKDKQILRKMINEGRXXXXXXXXXXXXXXXSQYLDSFEEAVKSIPTTVFLSEIPINVRTLLEDSRCIDINRESKSFWILVAALKDFIRSEGNGSLPLRGEIPDMTADTGRYVAIQKIYRQASEKDIIHLEKHVYRICQTF--QIPVT----------------DELNRNSIARFARNCQHIQILSSYSLERELKTSPTHTEELV-----CPETDELSWYLALRAAVSFYTVERRWPGQRQIDSEIDVPHLR----KLLQQDMNACVKFKDASVRDDFLLEMCRFSGVQLHSVCAFIAGIAAQEVIKLITGQYVPVMQPLMYNAIRQTT 2009
EKTK+YDRQ+RLWG++GQ ALE AKVCL+ + GTEI+KNLILPG+GSFTI+DD+ V ED+ NNFF+ K + +SRA L ELNE+V ++IE+SV++L+ NP +F F+VVIACGL K L+DL LL+ +I L+VC S G++ LR++++EH V+E+ PD+ DLRLD P ++C + +L MS K+ ++ PW++IL+K+I ++ S + P +YK+K L+++I EG K E + + D+F+EA++ + T + +++P V+ + +D C + ESK FWIL A+++F+ +EG+GSLPLRG IPDMTAD+ RY+ +Q +YR S +DI + ++ + Q+ P++ D ++ + I F RN ++++ + SL +E S + +L E DEL Y+ LR F T R+PG E D+ L+ K+LQ+ V+DDF+ E CR+ +LHSV AF+ G AAQEVIK+I Q++PV +YNA++Q++
Sbjct: 15 EKTKKYDRQLRLWGDHGQSALEMAKVCLVNANATGTEIMKNLILPGVGSFTIIDDNKVGGEDVGNNFFLDKDSIGKSRAHAATEFLLELNEDVNGDFIEESVDNLLQNNPTFFNNFTVVIACGLTEKSLLDLANLLWDNDIPLLVCRSYGLIGYLRIVLKEHTVIESHPDSALDDLRLDSPFPGLSDYCKNLDLEAMSKKDHSHTPWLVILYKFIQDWKRSHNGELPKNYKEKNQLKELIREG--------IRKSENGVPEQEDNFDEAIRHVNTALTHTKVPSEVQQIFDDPCCRQLTSESKPFWILARAVQEFVANEGSGSLPLRGNIPDMTADSERYIQLQNVYRDQSNQDICTVTNYIQTLLQSLGRDEPLSVSSEHRSPSSSPGQPQDNISDSDIKLFCRNASFLRVIRTRSLSQEYAASCSKITDLASRLDNTDEDDELVHYIMLRVVDRFRTEFNRYPGAEPDSMEPDIAKLKGCLCKMLQE-----WGVNPICVKDDFVHEYCRYGASELHSVAAFMGGAAAQEVIKMIVCQFIPVNNTFIYNAMKQSS 558
BLAST of SMED30023745 vs. TrEMBL
Match: A0A0L8I6G5 (NEDD8-activating enzyme E1 regulatory subunit OS=Octopus bimaculoides OX=37653 GN=OCBIM_22032483mg PE=3 SV=1) HSP 1 Score: 414.075 bits (1063), Expect = 7.980e-134 Identity = 212/534 (39.70%), Postives = 323/534 (60.49%), Query Frame = -2
Query: 423 RYDRQIRLWGENGQIALENAKVCLIGSGCIGTEILKNLILPGIGSFTIVDDSVVTEEDLSNNFFVQKSDLNQSRAEVTARLLRELNEEVIVNYIEDSVESLMFRNPEYFLEFSVVIACGLNNKDLVDLDRLLYPENIGLIVCHSIGMVANLRVIIQEHPVVEAKPDNIKPDLRLDKPLWEFVEFCDSQNLHEMSYKELAYCPWIIILFKYITSFQSKF-AKWPDSYKDKQILRKMINEGRXXXXXXXXXXXXXXXSQYLDSFEEAVKSIPTTVFLSEIPINVRTLLEDSRCIDINRESKSFWILVAALKDFIRSEGNGSLPLRGEIPDMTADTGRYVAIQKIYRQASEKDIIHLEKHVYRICQTFQIPVTDELNRNSIARFARNCQHIQILSSYSLERELKTSPTHTEELVCPETDE----LSWYLALRAAVSFYTVERRWPGQRQIDSEIDV----PHLRKLLQQDMNACVKFKDASVRDDFLLEMCRFSGVQLHSVCAFIAGIAAQEVIKLITGQYVPVMQPLMYNAIRQTT 1997
RYDRQ+RLWG++GQ LENAKVCLI + GTEILKNL+LPGIGSFTIVD VT D+ NNFF+ K + QSRA V LL ELNEEV +Y+E+S E L+ NPE+F F++V+A L+ K L+ L L +I L++C S G + R+++ +H ++E+ PDN DLRLD P ++FCD NL++M KE ++ PW++I++KY+ ++ + + P +YK+K ++++MI G + E + +F+EAV+++ T++ + IP V+ + D C ++ +SK+FWI++ A+K+F +EG +LPLRG IPDMTAD+ RY+ +Q IYR+ + KD+ ++ V I Q P + + F +N ++++ SL E +EL DE + +YL LRAA F+ R+PG E DV H+ KLL + + ++DD++ EMCR+ +LHS+ A++ G+ +QE IK+ITGQ+VP L+YNA++Q T
Sbjct: 19 RYDRQLRLWGDHGQACLENAKVCLINATATGTEILKNLVLPGIGSFTIVDGDKVTGADVGNNFFLAKDTIGQSRAHVATDLLMELNEEVSGDYVEESPEKLLESNPEFFNRFTLVVATNLHEKPLLQLATHLREADIPLLICRSYGFIGYARIVVADHTIIESHPDNSHEDLRLDCPFPGLIDFCDKLNLNDMEKKEHSHVPWLVIIYKYLQKWKDEHNGEPPKNYKEKTLMKEMIKSG--------VLRNENGNPEEEQNFDEAVENLNTSLVRTHIPETVQKIFNDPSCTQMHADSKNFWIMLHAMKEFTENEGKNTLPLRGTIPDMTADSNRYIQLQNIYREQAHKDVTNVLHRVQNILQKIGKPYC-SITEQDVKLFCKNGSFLRVIHCRSLAEEYDPKSARIQELGLQLGDEEDSDIVFYLMLRAAERFFEEYSRYPGWYDDQVEGDVFKLKTHVSKLLME-----LGLSSTLIKDDYIHEMCRYGASELHSIAAYMGGVMSQEAIKIITGQFVPFNNTLVYNAMKQCT 538
BLAST of SMED30023745 vs. Ensembl Cavefish
Match: nae1 (NEDD8 activating enzyme E1 subunit 1 [Source:NCBI gene;Acc:103042317]) HSP 1 Score: 367.851 bits (943), Expect = 2.049e-119 Identity = 194/537 (36.13%), Postives = 316/537 (58.85%), Query Frame = -2
Query: 423 KTKRYDRQIRLWGENGQIALENAKVCLIGSGCIGTEILKNLILPGIGSFTIVDDSVVTEEDLSNNFFVQKSDLNQSRAEVTARLLRELNEEVIVNYIEDSVESLMFRNPEYFLEFSVVIACGLNNKDLVDLDRLLYPENIGLIVCHSIGMVANLRVIIQEHPVVEAKPDNIKPDLRLDKPLWEFVEFCDSQNLHEMSYKELAYCPWIIILFKYITSFQSKF-AKWPDSYKDKQILRKMINEGRXXXXXXXXXXXXXXXSQYLDSFEEAVKSIPTTVFLSEIPINVRTLLEDSRCIDINRESKSFWILVAALKDFIRSEGNGSLPLRGEIPDMTADTGRYVAIQKIYRQASEKDIIHLEKHVYRICQTFQIPVTDELNRNSIARFARNCQHIQILSSYSLERELKTSP----THTEELVCPETDELSWYLALRAAVSFYTVERRWPGQRQIDSEIDVPHLR----KLLQQDMNACVKFKDASVRDDFLLEMCRFSGVQLHSVCAFIAGIAAQEVIKLITGQYVPVMQPLMYNAIRQTT 2006
K +RYDRQ+RLWG++GQ ALENA CL+ + GTEILKNLILPGIG+FTIVD VT ED+ NNFF+ + ++RA+ L+ELN +V N++E+S + L+ +PE+F FS+VI L L +L+ + +VC + G+V +R++++EH VVE+ PDN DLRLD+P E S +L M K+ ++ PWII++ KY+ + ++ + P +YK+K++ R++I +G K E + ++F+EA+K++ T + ++I V + +C +I ++ +FW++ ++DF+++EGNGSLP+RG IPDM AD+ +++ +Q +YR+ + +D + KH+ Q P ++ ++ I F ++ ++++ SL E T T T + P++ E+ YL LR+ FY R+PG E D+ L+ LLQ+ + +V+DD++ E CR+ + H+V +F+ G AAQE IKLIT Q+VP +YNA+ QT+
Sbjct: 8 KEQRYDRQLRLWGDHGQEALENAHTCLLNASATGTEILKNLILPGIGAFTIVDGHKVTGEDVGNNFFLSCESIGKNRAQAATEFLQELNSDVSGNFVEESPDKLLDNDPEFFHRFSLVIGVQLPESTCQRLGAVLWSAGVPFLVCRTYGLVGYMRLVVKEHTVVESHPDNALEDLRLDQPFTELRNHISSYHLDSMDKKDHSHTPWIIVIAKYLEKWYNEHNCQLPKNYKEKEVFRQLIRDG--------IRKNENGAPEEEENFDEAIKNVNTALNPTKISSAVDDIFNCVQCENITSQTSAFWMMARGVRDFVQNEGNGSLPVRGTIPDMIADSDKFINLQNVYREKALQDAAVVSKHIQSHLQAVGKP-SESISEQDIKLFCKSAAFLRVVRCRSLAEEYSTDTFQKDTITSCMDSPDS-EMVLYLMLRSVDRFYQQHSRYPGVYNYQVEEDISKLKLCVNSLLQEHGLS------VNVKDDYVHEFCRYGTAEPHTVASFLGGAAAQEAIKLITHQFVPFNNTFIYNAMSQTS 528
BLAST of SMED30023745 vs. Ensembl Cavefish
Match: nae1 (NEDD8 activating enzyme E1 subunit 1 [Source:NCBI gene;Acc:103042317]) HSP 1 Score: 323.168 bits (827), Expect = 2.643e-102 Identity = 185/542 (34.13%), Postives = 298/542 (54.98%), Query Frame = -2
Query: 423 KTKRYDRQIRLWGENGQIALENAKVCLIGSGCIGTEILKNLILPGIGSFTIVDDSVVTEEDLSNNFFVQKSDLNQSRAEVTARLLRELNEEVIVNYIEDSVESLMFRNPEYFLEFSVVIACGLNNKDLVDLDRLLYPENIGLIVCHSIGMVANLRVIIQEHPVVEAKPDNIKPDLRLDKPLWEFVEFCDSQNLHEMSYKEL---AYCPWIIILFK-----YITS--FQSKFAKWPDSYKDKQILRKMINEGRXXXXXXXXXXXXXXXSQYLDSFEEAVKSIPTTVFLSEIPINVRTLLEDSRCIDINRESKSFWILVAALKDFIRSEGNGSLPLRGEIPDMTADTGRYVAIQKIYRQASEKDIIHLEKHVYRICQTFQIPVTDELNRNSIARFARNCQHIQILSSYSLERELKTSPTHTEELVCPETDELSWYLALRAAVSFYTVERRWPGQRQIDSEIDVPHLR----KLLQQDMNACVKFKDASVRDDFLLEMCRFSGVQLHSVCAFIAGIAAQEVIKLITGQYVPVMQPLMYNAIRQTT 2006
K +RYDRQ+RLWG++GQ ALENA CL+ + GTEILKNLILPGIG+FTIVD VT ED+ N +A+ L+ELN +V N++E+S + L+ +PE+F FS+VI L L +L+ + +VC + G+V +R++++EH VVE+ PDN DLRLD+P E S +L M K L + ++F Y+ S ++ + P +YK+K++ R++I +G K E + ++F+EA+K++ T + ++I V + +C +I ++ +FW++ ++DF+++EGNGSLP+RG IPDM AD+ +++ +Q +YR+ + +D + KH+ Q + N + R R + S+ + +++ TS + P++ E+ YL LR+ FY R+PG E D+ L+ LLQ+ +V+DD++ E CR+ + H+V +F+ G AAQE IKLIT Q+VP +YNA+ QT+
Sbjct: 9 KEQRYDRQLRLWGDHGQEALENAHTCLLNASATGTEILKNLILPGIGAFTIVDGHKVTGEDVGN------------KAQAATEFLQELNSDVSGNFVEESPDKLLDNDPEFFHRFSLVIGVQLPESTCQRLGAVLWSAGVPFLVCRTYGLVGYMRLVVKEHTVVESHPDNALEDLRLDQPFTELRNHISSYHLDSMDKKVLLLISNTTHTTVIFNISKKIYLISKNVENHNCQLPKNYKEKEVFRQLIRDG--------IRKNENGAPEEEENFDEAIKNVNTALNPTKISSAVDDIFNCVQCENITSQTSAFWMMARGVRDFVQNEGNGSLPVRGTIPDMIADSDKFINLQNVYREKALQDAAVVSKHIQSHLQAVGKVINTNKNHAAFLRVVRCRSLAEEYSTDTFQKDTITSC-----MDSPDS-EMVLYLMLRSVDRFYQQHSRYPGVYNYQVEEDISKLKLCVNSLLQE------HGLSVNVKDDYVHEFCRYGTAEPHTVASFLGGAAAQEAIKLITHQFVPFNNTFIYNAMSQTS 518
BLAST of SMED30023745 vs. Ensembl Cavefish
Match: nae1 (NEDD8 activating enzyme E1 subunit 1 [Source:NCBI gene;Acc:103042317]) HSP 1 Score: 273.478 bits (698), Expect = 9.285e-84 Identity = 150/473 (31.71%), Postives = 265/473 (56.03%), Query Frame = -2
Query: 423 NFFVQKSDLNQSRAEVTARLLRELNEEVIVNYIEDSVESLMFRNPEYFLEFSVVIACGLNNKDLVDLDRLLYPENIGLIVCHSIGMVANLRVIIQEHPVVEAKPDNIKPDLRLDKPLWEFVEFCDSQNLHEMSYKELAYCPWIIILFKYITSFQSKF-AKWPDSYKDKQILRKMINEGRXXXXXXXXXXXXXXXSQYLDSFEEAVKSIPTTVFLSEIPINVRTLLEDSRCIDINRESKSFWILVAALKDFIRSEGNGSLPLRGEIPDMTADTGRYVAIQKIYRQASEKDIIHLEKHVYRICQTFQIPVTDELNRNSIARFARNCQHIQILSSYSLERELKTSP----THTEELVCPETDELSWYLALRAAVSFYTVERRWPGQRQIDSEIDVPHLR----KLLQQDMNACVKFKDASVRDDFLLEMCRFSGVQLHSVCAFIAGIAAQEVIKLITGQYVPVMQPLMYNAIRQTT 1814
+FF+ + ++RA+ L+ELN +V N++E+S + L+ +PE+F FS+VI L L +L+ + +VC + G+V +R++++EH VVE+ PDN DLRLD+P E S +L M K+ ++ PWII++ KY+ + ++ + P +YK+K++ R++I +G K E + ++F+EA+K++ T + ++I V + +C +I ++ +FW++ ++DF+++EGNGSLP+RG IPDM AD+ +++ +Q +YR+ + +D + KH+ Q P ++ ++ I F ++ ++++ SL E T T T + P++ E+ YL LR+ FY R+PG E D+ L+ LLQ+ + +V+DD++ E CR+ + H+V +F+ G AAQE IKLIT Q+VP +YNA+ QT+
Sbjct: 29 SFFLSCESIGKNRAQAATEFLQELNSDVSGNFVEESPDKLLDNDPEFFHRFSLVIGVQLPESTCQRLGAVLWSAGVPFLVCRTYGLVGYMRLVVKEHTVVESHPDNALEDLRLDQPFTELRNHISSYHLDSMDKKDHSHTPWIIVIAKYLEKWYNEHNCQLPKNYKEKEVFRQLIRDG--------IRKNENGAPEEEENFDEAIKNVNTALNPTKISSAVDDIFNCVQCENITSQTSAFWMMARGVRDFVQNEGNGSLPVRGTIPDMIADSDKFINLQNVYREKALQDAAVVSKHIQSHLQAVGKP-SESISEQDIKLFCKSAAFLRVVRCRSLAEEYSTDTFQKDTITSCMDSPDS-EMVLYLMLRSVDRFYQQHSRYPGVYNYQVEEDISKLKLCVNSLLQEHGLS------VNVKDDYVHEFCRYGTAEPHTVASFLGGAAAQEAIKLITHQFVPFNNTFIYNAMSQTS 485
BLAST of SMED30023745 vs. Ensembl Cavefish
Match: uba1 (ubiquitin like modifier activating enzyme 1 [Source:NCBI gene;Acc:103045813]) HSP 1 Score: 70.8626 bits (172), Expect = 3.654e-12 Identity = 44/160 (27.50%), Postives = 77/160 (48.12%), Query Frame = -2
Query: 1515 YDRQIRLWGENGQIALENAKVCLIGSGCIGTEILKNLILPGIGSFTIVDDSVVTEEDLSNNFFVQKSDLNQSRAEVTARLLRELNEEVIVNYIEDSVESLMFRNPEYFLEFSVVIACGLNNKDLVDLDRLLYPENIGLIVCHSIGMVANLRVIIQEHPVV 1994
Y RQ+ + G + ++++ V + G +G EI KN+IL G+ + T+ D V DLS+ F++++ DL ++RAEV+ L ELN V V + +Y +F VV+ + + + + I LI+ + G+ L E +V
Sbjct: 55 YSRQLYVLGHDAMKRMQSSNVLISGLRGLGVEIAKNVILGGVKTVTLHDQGVAEWNDLSSQFYLREEDLGKNRAEVSQTRLAELNSYVPVTAYTGELTD------DYLTDFQVVVLTSSTIDEQTRIGEFCHSKGIKLIIADTRGLFGQLFCDFGEEMIV 208 HSP 2 Score: 57.3806 bits (137), Expect = 4.940e-8 Identity = 41/165 (24.85%), Postives = 82/165 (49.70%), Query Frame = -2
Query: 1533 KTKRYDRQIRLWGENGQIALENAKVCLIGSGCIGTEILKNLILPGI----GSFTIVDDSVVTEEDLSNNFFVQKSDLNQSRAEVTARLLRELNEEVIVNYIEDSV--ESLMFRNPEYFLEFSVVIACGLNNKDL-VDLDRLLYPENIGLIVCHSIGMVANLRVII 2006
K+ RYD QI ++G Q L + L+G+G IG E+LKN + G+ G + D + + +L+ F + D+ + ++E A ++++N V + ++ V ++ + ++F V A L+N D + +DR L+ ++G N++V++
Sbjct: 448 KSCRYDGQIAVFGSKMQELLAKQRYFLVGAGAIGCELLKNFAMMGLASGEGEVIVTDMDTIEKSNLNRQFLFRPWDVTKMKSETAAAAVKQMNPSVRITGHQNRVGPDTEKVYDDDFFESLDGV-ANALDNVDARMYMDRRCVYYRKPLLESGTLGTKGNVQVVM 611
BLAST of SMED30023745 vs. Ensembl Cavefish
Match: uba7 (ubiquitin-like modifier activating enzyme 7 [Source:ZFIN;Acc:ZDB-GENE-121120-4]) HSP 1 Score: 68.5514 bits (166), Expect = 1.924e-11 Identity = 44/139 (31.65%), Postives = 70/139 (50.36%), Query Frame = -2
Query: 1545 ALENAKVCLIGSGCIGTEILKNLILPGIGSFTIVDDSVVTEEDLSNNFFVQKSDLNQSRAEVTARLLRELNEEVIVNYIEDSVESLMFRNPEYFLEFSVVIACGLNNKDLVD---LDRLLYPENIGLIVCHSIGMVANL 1952
++ A V + G +G E+ KN++L G+GS TI D DLS+ F++++SDL Q+RAE + + L LN V V+ + + N E+ F VV+ L N L + + + NI IV + G+ L
Sbjct: 3 SMGKANVLISGMRGLGVEVAKNVVLAGVGSVTIQDQGSAEWADLSSQFYLRESDLGQNRAECSEKPLARLNSHVRVSACTERL------NQEFLRNFQVVV---LTNSSLEEQRWVGSFCHTNNIRFIVADTKGLCGQL 132 HSP 2 Score: 56.9954 bits (136), Expect = 7.083e-8 Identity = 37/131 (28.24%), Postives = 65/131 (49.62%), Query Frame = -2
Query: 1626 RYDRQIRLWGENGQIALENAKVCLIGSGCIGTEILKNLILPGIGS-----FTIVDDSVVTEEDLSNNFFVQKSDLNQSRAEVTARLLRELNEEVIVNYIEDSV--ESLMFRNPEYFLEFSVVIACGLNNKD 1997
RYD QI ++G + Q L+ + L+G+G IG E+LKN L G+G+ T+ D + +L+ F + D+ ++ A ++ +N V + ++ V ES + ++F V A L+N D
Sbjct: 386 RYDGQIAVFGADFQEKLKKQRYFLVGAGAIGCELLKNFALIGLGAGEGGHITVTDMDSIERSNLNRQFLFRSQDIGTQKSVAAAEAVKNMNPNVNITAHQNRVCPESEGIYSYQFFSSLDGV-AAALDNVD 515
BLAST of SMED30023745 vs. Ensembl Sea Lamprey
Match: nae1 (nedd8 activating enzyme E1 subunit 1 [Source:NCBI gene;Acc:573336]) HSP 1 Score: 341.658 bits (875), Expect = 2.141e-110 Identity = 187/508 (36.81%), Postives = 294/508 (57.87%), Query Frame = -2
Query: 513 EKTKRYDRQIRLWGENGQIALENAKVCLIGSGCIGTEILKNLILPGIGSFTIVDDSVVTEEDLSNNFFVQKSDLNQSRAEVTARLLRELNEEVIVNYIEDSVESLMFRNPEYFLEFSVVIACGLNNKDLVDLDRLLYPENIGLIVCHSIGMVANLRVIIQEHPVVEAKPDNIKPDLRLDKPLWEFVEFCDSQNLHEMSYKELAYCPWIIILFKYITSFQSKF-AKWPDSYKDKQILRKMINEGRXXXXXXXXXXXXXXXSQYLDSFEEAVKSIPTTVFLSEIPINVRTLLEDSRCIDINRESKSFWILVAALKDFIRSEGN-GSLPLRGEIPDMTADTGRYVAIQKIYRQASEKDIIHLEKHVYRICQTFQIPVTDELNRNSIARFARNCQHIQILSSYSLERELKTSPTHTEELVC---PETDELSWYLALRAAVSFYTVERRWPGQRQIDSEIDVPHLRK----LLQQDMNACVKFKDASVRDDFLLEMCRFSGVQLHSVCAFIAG 2009
EK +RYDRQ+RLWG++GQ ALENA VCLI + GTEILKNL+LPGIG+FTI+D + V+ ED+ NNFF+ KS + +SRA+ L++ELN +V N++E++VE ++ + P +F +F+VVI L L+ L L+ + L+ C + GM +RV ++EH VVE+ PDN DLRLD+P V+ S +L + KE ++ PW++++ KY+ ++S+ P +YK+K +++I +G + SS E ++FEEA+K+ T + + IP VR L D C ++ +S FW+LV AL+DF+ +EG G+LPLRG IPDM AD+ ++V +Q +YR+ +++D + K + + P D ++ + F ++ ++++ SL E T ++ E + LRA FY R+PG R D + DV LR LL + C +V+D++L E CR+ + H+V +FI G
Sbjct: 8 EKERRYDRQLRLWGDHGQQALENAHVCLINASATGTEILKNLVLPGIGAFTILDGNKVSGEDIGNNFFLDKSSIGESRAKTAMNLIQELNSDVSGNFVEEAVEKVLEKEPTFFHKFTVVITTQLPQSTLLRLAAALWEAGVPLLACRAYGMTGYMRVALREHTVVESHPDNALEDLRLDRPFPALVKHLASCDLALLDKKEHSHTPWLVVVAKYLDVWRSQHDGSLPKNYKEKDQFKELIRQGMIKNEHGASSVE--------ENFEEAIKNTTTALNPTRIPDVVRQLFSDPSCQSLSAKSADFWVLVRALRDFVEAEGGEGALPLRGSIPDMIADSDKFVRLQNVYREKAKQDADAVGKLTASLLHSLGRPA-DGISEGDVRLFCKHAAFLRVVRCRSLAEEYDAKTALTADIGSQLESAEGEAVLNIMLRAVDRFYQQYGRYPGVRAEDVDRDVAELRACVAGLLHEWGLHC------TVKDEYLHEFCRYGAAEPHTVASFIGG 500
BLAST of SMED30023745 vs. Ensembl Sea Lamprey
Match: sae1 (SUMO1 activating enzyme subunit 1 [Source:NCBI gene;Acc:415148]) HSP 1 Score: 97.0561 bits (240), Expect = 4.043e-22 Identity = 70/246 (28.46%), Postives = 115/246 (46.75%), Query Frame = -2
Query: 1281 EKTKRYDRQIRLWGENGQIALENAKVCLIGSGCIGTEILKNLILPGIGSFTIVDDSVVTEEDLSNNFFVQKSDLNQSRAEVTARLLRELNEEVIVNYIEDSVESLMFRNPEYFLEFSVVIACGLNNKDLVDLDRLLYPENIGLIVCHSIGMVANLRVIIQEHPVVEAKPDNIKPDLRLDKPLWEFVEFCDSQNLHEMSYKELAYCPWIIILFKYITSFQSK--FAKWPDSY-KDKQILRKMINEGR 2009
E+ +YDRQIRLWG Q L +K+ ++G +G E+ KNLIL G+ S T +D++ TEE F V +S L ++ AE + + LN V V + + S + E+F +F + G ++ ++R+ + NI G + + H VE KP ++ + D P + + S++ M K + +C + L T+ Q+K K P Y K +LR +GR
Sbjct: 15 EEAAQYDRQIRLWGLEAQKRLRASKLLIVGMKGLGAEVAKNLILAGVKSVTFLDNNKATEESTRAQFLVPQSRLGENLAEASLERAQNLNPMVDVRTDKSDIAS---KTEEFFTQFDSICLTGCPQDVMIKINRICHERNIKFFAGDVFGYHGYMFADLGIHDYVEEKPKKVEVEGAEDGPAAKKRKVISSEST--MVKKTVEFCTLVEALDADWTTDQAKKIIKKTPAGYFLTKVLLRFRTEQGR 255
BLAST of SMED30023745 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000009036.1 (pep scaffold:Pmarinus_7.0:GL491851:103397:109134:1 gene:ENSPMAG00000008176.1 transcript:ENSPMAT00000009036.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 52.7582 bits (125), Expect = 3.093e-8 Identity = 39/150 (26.00%), Postives = 77/150 (51.33%), Query Frame = -2
Query: 1545 YDRQIRLWGENGQIALENAKVCLIGSGCIGTEILKNLILPGIGSFTIVDDSVVTEEDLSNNFFVQKSDLNQSRAEVTARLLRELNEEVIVNYIEDSVESLMFRNPEYFLEFSVVIACGLNNKDLVDLDRLLYPENIGLIVCHSIGMVANL 1994
+ RQ+ + G ++ A V + G +G E+ KN++L G+G+ T+ D + DLS+ F+ ++ DL ++RA+++ L++LNE V V+ DS+ E+ +F VV+ + + + + I LI+ + G+ +
Sbjct: 13 FSRQLYVLGHEATRRIQEAHVLVSGMHGLGLEVAKNVVLVGVGTVTVHDTQPLARRDLSSQFYGREEDLKRNRAQLSQSHLKQLNEFVRVSDYSDSL------TEEFLSQFQVVVLTNSSLSEQLRIGSFCRAHGIKLIIASTRGLFGQI 156
BLAST of SMED30023745 vs. Ensembl Yeast
Match: AOS1 (Subunit of heterodimeric nuclear SUMO activating enzyme E1 with Uba2p; activates Smt3p (SUMO) before its conjugation to proteins (sumoylation), which may play a role in protein targeting; essential for viability; relocalizes to the cytosol in response to hypoxia [Source:SGD;Acc:S000006384]) HSP 1 Score: 108.997 bits (271), Expect = 2.329e-26 Identity = 59/169 (34.91%), Postives = 98/169 (57.99%), Query Frame = -2
Query: 1488 YDRQIRLWGENGQIALENAKVCLIGSGCIGTEILKNLILPGIGSFTIVDDSVVTEEDLSNNFFVQKSDLNQSRAEVTARLLRELNEEVIVNYIEDSVESLMFRNPEYFLEFSVVIACGLNNKDLVDLDRLLYPENIGLIVCHSIGMVANLRVIIQEHPVVEAKPDNIKP 1994
YDRQIRLWG Q + +AKV LI G IG+EI K+++L GIG TI+D +VTEEDL + FF+ D+ Q + + T +++LN + +N+ + L ++ E+F +F +V+A + + + ++ L NI L V S G+ A + + + E + K +++P
Sbjct: 16 YDRQIRLWGMTAQANMRSAKVLLINLGAIGSEITKSIVLSGIGHLTILDGHMVTEEDLGSQFFIGSEDVGQWKIDATKERIQDLNPRIELNF---DKQDLQEKDEEFFQQFDLVVATEMQIDEAIKINTLTRKLNIPLYVAGSNGLFAYVFIDLIEFISEDEKLQSVRP 181
BLAST of SMED30023745 vs. Ensembl Yeast
Match: ULA1 (Protein that activates Rub1p (NEDD8) before neddylation; acts together with Uba3p; may play a role in protein degradation [Source:SGD;Acc:S000005924]) HSP 1 Score: 88.9669 bits (219), Expect = 3.351e-19 Identity = 91/375 (24.27%), Postives = 170/375 (45.33%), Query Frame = -2
Query: 921 KRYDRQIRLWGENGQIALENAKVCLIGSGC-IGTEILKNLILPGIGSFTIVDDSVVTEEDLSNNFFVQKSDLNQSRAEVTARLLRELNEEVIVNYIEDSVESLMFRNPEY-FLEFSVVI-ACGLNNKDLVDLDRLL------YPENIGLIVCHSIGMVANLRVIIQEHPVVEAKPDNIKPDLRLDKPLWEFVEFCDSQNLHEMSYKELAYCPWIIILFKYITSFQSKFAKWPDSYKDKQILRKMINEGRXXXXXXXXXXXXXXXSQYLDSFEEAVKSIPTTVFLSEIPINVRTLLEDSRCIDINRESKSFW-----ILVAALKDFIRSEGNGSL-PLRGEIPDMTADTGRYVAIQKIYRQASEKDIIHLEKHVYR 2000
+RYDRQ+RLWG GQ +L ++VC++G + E+ KNL+L GI S T + + S + F+ ++L + + ++ L Y E+ + + + P+Y + FSVVI C ++DL+ + +P + V G + V+ + H V++A PD+ K DLRL P E + + D+ +L +M + P+ ++L K I + +D R I++ ++ ++C + Y ++ EA + ++ + LL + D + + L+ LK+ + G S PL G +PDM + T Y+ ++K+Y ++ D +E+ + R
Sbjct: 2 ERYDRQLRLWGALGQDSLNRSRVCVVGPATPLLQEVFKNLVLAGISSLTWLKVECAVQ---SGSLFL--AELKKDLEPLASKQLE---------YEENDLRKTL-QQPQYDWTRFSVVILTCIGEQTAMLDLNEIRRQRGTKFPPVLNTFVSGFYGYI--YLVLSETHFVLQAHPDSKKYDLRLQNPWPELINYVDTFDLSKMDTATFSGIPYTVLLMKCIAKLE----------RDGNNGRITIDQMKKVLDQICLPLGNDVI--YEPNYVEAKRYAYLACSQNDCCKELEDLLRNLEISDYGNDWHDTYNYEILTLLLTLKNIAKENGELSFQPLTGTLPDMESTTENYIRLKKLYEVKAKLDKSRVEESLAR 347
BLAST of SMED30023745 vs. Ensembl Yeast
Match: UBA1 (Ubiquitin activating enzyme (E1); involved in ubiquitin-mediated protein degradation and essential for viability; protein abundance increases in response to DNA replication stress [Source:SGD;Acc:S000001693]) HSP 1 Score: 74.7146 bits (182), Expect = 2.757e-14 Identity = 47/153 (30.72%), Postives = 79/153 (51.63%), Query Frame = -2
Query: 1539 YDRQIRLWGENGQIALENAKVCLIGSGCIGTEILKNLILPGIGSFTIVDDSVVTEEDLSNNFFVQKSDLNQSRAEVTARLLRELNEEVIVNYIEDSVESLMFRNPEYFLEFSVVIACG-LNNKDLVDLDRLLYPENIGLIVCHSIGMVANLRV 1994
Y RQ+ + G+ + ++ + V ++G +G EI KN++L G+ S T+ D V DLS FF+ + D+ Q R +VT L ELN V VN + DS++ + +F VV+A ++ +D V ++ + I I + G+ N V
Sbjct: 19 YSRQLYVLGKEAMLKMQTSNVLILGLKGLGVEIAKNVVLAGVKSMTVFDPEPVQLADLSTQFFLTEKDIGQKRGDVTRAKLAELNAYVPVNVL-DSLDDVT-----QLSQFQVVVATDTVSLEDKVKINEFCHSSGIRFISSETRGLFGNTFV 165 HSP 2 Score: 62.7734 bits (151), Expect = 1.411e-10 Identity = 32/94 (34.04%), Postives = 56/94 (59.57%), Query Frame = -2
Query: 1731 RYDRQIRLWGENGQIALENAKVCLIGSGCIGTEILKNLILPGIGS-----FTIVDDSVVTEEDLSNNFFVQKSDLNQSRAEVTARLLRELNEEV 1997
RYD QI ++G + Q + N+KV L+GSG IG E+LKN L G+GS + D+ + + +L+ F + D+ ++++EV A + +N ++
Sbjct: 416 RYDNQIAVFGLDFQKKIANSKVFLVGSGAIGCEMLKNWALLGLGSGSDGYIVVTDNDSIEKSNLNRQFLFRPKDVGKNKSEVAAEAVCAMNPDL 509
BLAST of SMED30023745 vs. Ensembl Yeast
Match: UBA4 (E1-like protein that activates Urm1p before urmylation; also acts in thiolation of the wobble base of cytoplasmic tRNAs by adenylating and then thiolating Urm1p; receives sulfur from Tum1p [Source:SGD;Acc:S000001153]) HSP 1 Score: 53.9138 bits (128), Expect = 5.379e-8 Identity = 32/102 (31.37%), Postives = 51/102 (50.00%), Query Frame = -2
Query: 1719 LEKTKRYDRQI---RLWGENGQIALENAKVCLIGSGCIGTEILKNLILPGIGSFTIVDDSVVTEEDLSNNFFVQKSDLNQSRAEVTARLLRELNEEV-IVNY 2012
LE+ +RY RQ+ G GQ+ L+N KV ++G+G +G L L G+G IVD+ VV +L S + + E + + +LN + +V Y
Sbjct: 41 LEEYQRYGRQMIVEETGGVAGQVKLKNTKVLVVGAGGLGCPALPYLAGAGVGQIGIVDNDVVETSNLHRQVLHDSSRVGMLKCESARQYITKLNPHINVVTY 142
BLAST of SMED30023745 vs. Ensembl Yeast
Match: UBA2 (Subunit of heterodimeric nuclear SUMO activating enzyme E1 with Aos1p; activates Smt3p (SUMO) before its conjugation to proteins (sumoylation), which may play a role in protein targeting; essential for viability [Source:SGD;Acc:S000002798]) HSP 1 Score: 51.6026 bits (122), Expect = 3.324e-7 Identity = 30/117 (25.64%), Postives = 61/117 (52.14%), Query Frame = -2
Query: 1632 IRLWGENGQIALENAKVCLIGSGCIGTEILKNLILPGIGSFTIVDDSVVTEEDLSNNFFVQKSDLNQSRAEVTARLLRELNEEVIVNYIEDSVESLMFRNPEYFLEFSVVIACGLNN 1982
+ + GE+ L +++ L+G+G IG+E+LK++IL G IVD + +L+ F ++ D+ Q ++ + ++ N +V Y + ++ F P ++ E +I L+N
Sbjct: 8 VTIIGEDSYKKLRSSRCLLVGAGGIGSELLKDIILMEFGEIHIVDLDTIDLSNLNRQFLFRQKDIKQPKSTTAVKAVQHFNNSKLVPYQGNVMDISTF--PLHWFEQFDIIFNALDN 122
BLAST of SMED30023745 vs. Ensembl Nematostella
Match: EDO44524 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RV71]) HSP 1 Score: 387.497 bits (994), Expect = 1.741e-127 Identity = 201/535 (37.57%), Postives = 323/535 (60.37%), Query Frame = -2
Query: 423 LEKTKRYDRQIRLWGENGQIALENAKVCLIGSGCIGTEILKNLILPGIGSFTIVDDSVVTEEDLSNNFFVQKSDLNQSRAEVTARLLRELNEEVIVNYIEDSVESLMFRNPEYFLEFSVVIACGLNNKDLVDLDRLLYPENIGLIVCHSIGMVANLRVIIQEHPVVEAKPDNIKPDLRLDKPLWEFVEFCDSQNLHEMSYKELAYCPWIIILFKYITSFQSKF-AKWPDSYKDKQILRKMINEGRXXXXXXXXXXXXXXXSQYLDSFEEAVKSIPTTVFLSEIPINVRTLLEDSRCIDINRESKSFWILVAALKDFIRSEGNGSLPLRGEIPDMTADTGRYVAIQKIYRQASEKDIIHLEKHVYRICQTFQIPVTDELNRNSIARFARNCQHIQILSSYSLERELKTSPTHTEE----LVCPETDELSWYLALRAAVSFYTVERRWPGQRQIDSEIDVPHLRKLLQQDMNACVKFKDASVRDDFLLEMCRFSGVQLHSVCAFIAGIAAQEVIKLITGQYVPVMQPLMYNAIRQTT 2012
+EK K+YDRQ+RLWG++GQ ALE AK+CLI + GTEILKNLILPGIG FTIVD VT ED+ NNFF+ K + +SRAE LL ELN +V +IE+S E L+ ++F FSVVIA L+ + L+ L +L+ + + L+VC S G + +R++++EHP++E+ PD+ DLRLD+P ++ DS +L MS + ++ P+++IL KY+ ++ + K P +YK+K +K++ +G E + + ++F+EA++++ + ++IP V + C +I S SFWI+ A+K+F+ +EG G+LP+RG IPDMTAD+ RY+ +Q +Y+ ++KD++ + + V+RI T D + I F +N ++++ SL+ E S + T E L PE+D + +Y+ LRA FY+ R+PG E DV L+ + + +A+V+ + + E+CR+ +LH V AF+ G AAQEV+K++T Q+VP +YN + ++
Sbjct: 6 VEKEKKYDRQLRLWGDHGQAALEQAKICLINASATGTEILKNLILPGIGLFTIVDGEKVTGEDVGNNFFLNKEHIGRSRAECATELLLELNSDVSGEFIEESPEELLTHKQDFFKPFSVVIATQLSEEILLKLAHVLWSDKVPLLVCRSYGFIGYMRLVLEEHPIIESHPDSAFEDLRLDRPFPTLQQYVDSMDLESMSKLDHSHTPYVVILLKYLQRWKEEHDNKPPQNYKEKDAFKKIVRKG--------ILINEHGIPEEEENFDEAIRAVNMALVPTKIPGEVSNIFSQESCCNITEMSSSFWIIAKAVKEFVENEGQGALPVRGSIPDMTADSKRYIQLQNVYQDQAKKDVVAVTQRVHRILDTIG-KSADCIPEQEIRLFCKNAAFLKLVRCRSLQAEYDPSTSRTNEIGNALDDPESD-VMFYVLLRAVDRFYSQYHRYPGNFDDQVEADVVKLKSCVAAQLQEW-SLPNATVKSECIHEICRYGASELHPVAAFMGGAAAQEVVKIVTRQFVPFSNTFIYNGMTGSS 529
BLAST of SMED30023745 vs. Ensembl Nematostella
Match: EDO49380 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RG90]) HSP 1 Score: 99.3673 bits (246), Expect = 1.325e-22 Identity = 67/245 (27.35%), Postives = 113/245 (46.12%), Query Frame = -2
Query: 1320 YDRQIRLWGENGQIALENAKVCLIGSGCIGTEILKNLILPGIGSFTIVDDSVVTEEDLSNNFFVQKSDLNQSRAEVTARLLRELNEEVIVNYIEDSVESLMFRNPEYFLEFSVVIACGLNNKDLVDLDRLLYPENIGLIVCHSIGMVANLRVIIQEHPVVEAKPDNIKP---------------DLRLDKPLWEFVEFCDSQN-----LHEMSYKELAYCPWIIILFKYITSFQSKFAKWPDSYK 1994
YDRQIRLWG + Q L +++ ++G IG EI KNL+L G+ S T++D++ VTE D + F + L ++RAE + + LN V V+ ++++ + + + +F VV+A G ++ LV + +NI G + + +H VE + I D K + +F EF +N E K L P + + + I F++K + PDS +
Sbjct: 18 YDRQIRLWGLDAQKRLRASRILVVGLAGIGAEICKNLVLSGVKSLTMLDNNPVTERDFVSQFLAPREALGKNRAEASLARTQALNPMVAVSADKNNITA---KADTFLDDFDVVVATGCSSDILVSIYERCRAKNIKFFASDVFGFYGYMFADLGKHRYVEEERKTIHSAEKKEKEPAKKKQKIDSTETKTVEKFCEFSSLKNSLSCSFSETRVKSLKRLPSVYFILQVILRFRAKHGRAPDSLQ 259
BLAST of SMED30023745 vs. Ensembl Nematostella
Match: EDO33332 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SSG6]) HSP 1 Score: 62.7734 bits (151), Expect = 3.927e-10 Identity = 41/150 (27.33%), Postives = 75/150 (50.00%), Query Frame = -2
Query: 1545 YDRQIRLWGENGQIALENAKVCLIGSGCIGTEILKNLILPGIGSFTIVDDSVVTEEDLSNNFFVQKSDLNQSRAEVTARLLRELNEEVIVNYIEDSVESLMFRNPEYFLEFSVVIACGLNNKDLVDLDRLLYPENIGLIVCHSIGMVANL 1994
Y RQ+ + G + + V + G +G EI KN++L G+ S T+ D V DL++ FF+ K D+ ++RAEV+ + ELN Y+ SV + E+ +F VV+ + ++ + + + + I LI+ + G+ +
Sbjct: 8 YSRQLYVLGHEAMKKMAVSNVLISGLRGLGVEIAKNVVLGGVKSVTLHDTGAVELSDLTSQFFLNKKDVGKNRAEVSHPRIAELN-----TYVSMSVNTQKLTE-EFINKFQVVVLTESSLEEQLWISDFCHSKGIKLIISDTKGLFGQI 151 HSP 2 Score: 61.6178 bits (148), Expect = 7.785e-10 Identity = 45/163 (27.61%), Postives = 81/163 (49.69%), Query Frame = -2
Query: 1533 RYDRQIRLWGENGQIALENAKVCLIGSGCIGTEILKNLILPGIGS-----FTIVDDSVVTEEDLSNNFFVQKSDLNQSRAEVTARLLRELNEEVIVNYIEDSV--ESLMFRNPEYFLEFSVVIACGLNNKDL-VDLDRLLYPENIGLIVCHSIGMVANLRVII 1997
RYD QI ++G Q L K ++G+G IG E LKN + G+GS D + + +L+ F + +D+ + ++EV AR ++ +N +V + + V E+ N ++F S V L+N D + +DR L+ ++G N++V++
Sbjct: 400 RYDGQIAVFGSEFQKKLGQQKYFVVGAGAIGCEHLKNFAMMGLGSGEGGHIYTTDMDTIEKSNLNRQFLFRPADVQKMKSEVAARAVKVMNPDVNITAHGNRVGPETEKVYNDDFFESLSGVTN-ALDNVDARMYMDRRCVYYRKPLLESGTLGTKGNVQVVL 561
BLAST of SMED30023745 vs. Ensembl Nematostella
Match: EDO46454 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RPW5]) HSP 1 Score: 60.4622 bits (145), Expect = 1.796e-9 Identity = 35/138 (25.36%), Postives = 69/138 (50.00%), Query Frame = -2
Query: 1536 LENAKVCLIGSGCIGTEILKNLILPGIGSFTIVDDSVVTEEDLSNNFFVQKSDLNQSRAEVTARLLRELNEEVIVNYIEDSVESLMFRNPEYFLEFSVVIACGLNNKDLVDLDRLLYPENIGLIVCHSIGMVANLRVI 1949
+ N+K+ ++G+G IG E+LKNL+L G + ++D + +L+ F K + +S+A+V N + + I D++ S + +YF +F VV+ N ++R+ ++ L+ + G + VI
Sbjct: 21 VNNSKILVVGAGGIGCELLKNLVLTGFKNIDLIDLDTIDVSNLNRQFLFHKQHVGKSKAKVARESALRFNPDAKIVAIHDNITSPEY-GIDYFKQFDVVMNALDNRAARNHVNRMCLAADVPLVESGTAGYLGQTTVI 157
BLAST of SMED30023745 vs. Ensembl Nematostella
Match: EDO28720 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T5P2]) HSP 1 Score: 53.5286 bits (127), Expect = 3.365e-8 Identity = 38/144 (26.39%), Postives = 71/144 (49.31%), Query Frame = -2
Query: 1545 LWGENGQIALENAKVCLIGSGCIGTEILKNLILPGIGSFTIVDDSVVTEEDLSNNFFVQKSDLNQSRAEVTARLLRELNEEVIVNYIEDSVESLMFRNPEYFLEFSVVIACGLNNKDLVDLDRLLYPENIGLIVCHSIGMVANL 1976
+ G + + V + G +G EI KN++L G+ S T+ D V DL++ FF+ K D+ ++RAEV+ + ELN Y+ SV + E+ +F VV+ + ++ + + + + I LI+ + G+ +
Sbjct: 16 VLGHEAMKKMAVSNVLISGLRGLGVEIAKNVVLGGVKSVTLHDTGAVELSDLTSQFFLNKKDVGKNRAEVSHPRIAELN-----TYVSMSVNTQKL-TEEFINKFQVVVLTESSLEEQLWISDFCHSKGIKLIISDTKGLFGQI 153
BLAST of SMED30023745 vs. Ensembl Medaka
Match: nae1 (NEDD8 activating enzyme E1 subunit 1 [Source:NCBI gene;Acc:101170334]) HSP 1 Score: 385.571 bits (989), Expect = 2.733e-126 Identity = 196/536 (36.57%), Postives = 321/536 (59.89%), Query Frame = -2
Query: 423 KTKRYDRQIRLWGENGQIALENAKVCLIGSGCIGTEILKNLILPGIGSFTIVDDSVVTEEDLSNNFFVQKSDLNQSRAEVTARLLRELNEEVIVNYIEDSVESLMFRNPEYFLEFSVVIACGLNNKDLVDLDRLLYPENIGLIVCHSIGMVANLRVIIQEHPVVEAKPDNIKPDLRLDKPLWEFVEFCDSQNLHEMSYKELAYCPWIIILFKYITSFQSKFA-KWPDSYKDKQILRKMINEGRXXXXXXXXXXXXXXXSQYLDSFEEAVKSIPTTVFLSEIPINVRTLLEDSRCIDINRESKSFWILVAALKDFIRSEGNGSLPLRGEIPDMTADTGRYVAIQKIYRQASEKDIIHLEKHVYRICQTFQIPVTDELNRNSIARFARNCQHIQILSSYSLERELKTSPTHTEELVC----PETDELSWYLALRAAVSFYTVERRWPGQRQIDSEIDVPHLR---KLLQQDMNACVKFKDASVRDDFLLEMCRFSGVQLHSVCAFIAGIAAQEVIKLITGQYVPVMQPLMYNAIRQTT 2006
K ++YDRQ+RLWG++GQ ALENA VCLI + GTEILKNL+LPGIG+FTIVD V+ ED NNFF+ + + ++RA+ LL+ELN +V N++E+S + L+ +PE+F F++VI L + L +L+ ++ ++C + G++ +R+++QEH V+E+ PDN DLRLD+P EF +S +L M K+ ++ PWIII+ KY+ + S+ + P +YK+K+ R+ I EG + E ++FEEA+K++ T + L++IP ++ L +C I +S FW+++ A+K+F+ EG GSLP+RG IPDM AD+ +++ +Q +YR+ + +D + KHV + ++ P + ++ I F +N ++++ SL E + +E+ P++ E+ +YL LRA FY R+PG E D+ L+ L Q+ + V +++DD++ E CR+ + H+V AF+ G AAQE IK+I+ Q+VP +YNA+ QT+
Sbjct: 8 KEQKYDRQLRLWGDHGQEALENAHVCLINATATGTEILKNLVLPGIGAFTIVDGHTVSGEDAGNNFFLSNNSIGRNRAQAATELLQELNGDVSGNFVEESPDKLLDNDPEFFHRFTIVIGVQLPESTCLRLGSVLWNASVPFLICKTYGLIGYMRLVVQEHTVIESHPDNALEDLRLDQPFEEFKNHTNSYDLDSMDKKDHSHTPWIIIVAKYLEKWLSEHNDQLPKNYKEKEAFRQTIREGTRKTDGGVPEDE--------ENFEEAIKNVNTALNLTKIPSSIENLFNSEQCNSITSQSSPFWVMLRAVKEFVHDEGKGSLPVRGTIPDMIADSQKFINLQNVYREKAMQDAAAVSKHVECLLRSVGKP-PESISEKDIKLFCKNASFLRVVRCRSLAEEYSVDSVNKDEIASYMDNPDS-EMVFYLMLRAVDRFYQQHSRYPGVYNYQVEEDINKLKVCVNSLLQEYSLSV-----NIKDDYIHEFCRYGAAEPHTVSAFLGGSAAQEAIKIISHQFVPFNNTFIYNAMSQTS 528
BLAST of SMED30023745 vs. Ensembl Medaka
Match: nae1 (NEDD8 activating enzyme E1 subunit 1 [Source:NCBI gene;Acc:101170334]) HSP 1 Score: 345.895 bits (886), Expect = 4.566e-111 Identity = 184/536 (34.33%), Postives = 310/536 (57.84%), Query Frame = -2
Query: 423 KTKRYDRQIRLWGENGQIALENAKVCLIGSGCIGTEILKNLILPGIGSFTIVDDSVVTEEDLSNNFFVQKSDLNQSRAEVTARLLRELNEEVIVNYIEDSVESLMFRNPEYFLEFSVVIACGLNNKDLVDLDRLLYPENIGLIVCHSIGMVANLRVIIQEHPVVEAKPDNIKPDLRLDKPLWEFVEFCDSQNLHEMSYKELAYCPWIIILFKYITSFQSKFA-KWPDSYKDKQILRKMINEGRXXXXXXXXXXXXXXXSQYLDSFEEAVKSIPTTVFLSEIPINVRTLLEDSRCIDINRESKSFWILVAALKDFIRSEGNGSLPLRGEIPDMTADTGRYVAIQKIYRQASEKDIIHLEKHVYRICQTFQIPVTDELNRNSIARFARNCQHIQILSSYSLERELKTSPTHTEELVC----PETDELSWYLALRAAVSFYTVERRWPGQRQIDSEIDVPHLR---KLLQQDMNACVKFKDASVRDDFLLEMCRFSGVQLHSVCAFIAGIAAQEVIKLITGQYVPVMQPLMYNAIRQTT 2006
K ++YDRQ+RLWG++GQ ALENA VCLI + GTEILKNL+LPG ++ ++ D + + +S+ RA+ LL+ELN +V N++E+S + L+ +PE+F F++VI L + L +L+ ++ ++C + G++ +R+++QEH V+E+ PDN DLRLD+P EF +S +L M K+ ++ PWIII+ KY+ + S+ + P +YK+K+ R+ I EG + E ++FEEA+K++ T + L++IP ++ L +C I +S FW+++ A+K+F+ EG GSLP+RG IPDM AD+ +++ +Q +YR+ + +D + KHV + ++ P + ++ I F +N ++++ SL E + +E+ P++ E+ +YL LRA FY R+PG E D+ L+ L Q+ + V +++DD++ E CR+ + H+V AF+ G AAQE IK+I+ Q+VP +YNA+ QT+
Sbjct: 8 KEQKYDRQLRLWGDHGQEALENAHVCLINATATGTEILKNLVLPG--TWEMLHDGDSHQSHHPTTCLIPRSN----RAQAATELLQELNGDVSGNFVEESPDKLLDNDPEFFHRFTIVIGVQLPESTCLRLGSVLWNASVPFLICKTYGLIGYMRLVVQEHTVIESHPDNALEDLRLDQPFEEFKNHTNSYDLDSMDKKDHSHTPWIIIVAKYLEKWLSEHNDQLPKNYKEKEAFRQTIREGTRKTDGGVPEDE--------ENFEEAIKNVNTALNLTKIPSSIENLFNSEQCNSITSQSSPFWVMLRAVKEFVHDEGKGSLPVRGTIPDMIADSQKFINLQNVYREKAMQDAAAVSKHVECLLRSVGKP-PESISEKDIKLFCKNASFLRVVRCRSLAEEYSVDSVNKDEIASYMDNPDS-EMVFYLMLRAVDRFYQQHSRYPGVYNYQVEEDINKLKVCVNSLLQEYSLSV-----NIKDDYIHEFCRYGAAEPHTVSAFLGGSAAQEAIKIISHQFVPFNNTFIYNAMSQTS 522
BLAST of SMED30023745 vs. Ensembl Medaka
Match: sae1 (SUMO1 activating enzyme subunit 1 [Source:NCBI gene;Acc:101162772]) HSP 1 Score: 83.5741 bits (205), Expect = 6.188e-17 Identity = 63/178 (35.39%), Postives = 89/178 (50.00%), Query Frame = -2
Query: 1488 EKTKRYDRQIRLWGENGQIALENAKVCLIGSGCIGTEILKNLILPGIGSFTIVDDSVVTEEDLSNNFFVQKSDLNQSRAEVTARLLRELNEEVIVNYIEDSVESLMFRNPEYFLEFSVVIACGLNNKDLVDLDRLLYPENIGLIVCHSIG----MVANLRVIIQEHPVVEAKPDNIKP 2009
E+ +YDRQIRLWG + Q L ++V L G G +G E+ KNLIL G+ T++D VTEE F V S Q+RA+ + + LN V V+ VE + ++FL+F V G + +V +D+L I + G M NL QE+ VE KP +KP
Sbjct: 12 EEAAQYDRQIRLWGLDAQKRLRGSRVLLAGLGGLGAEVAKNLILAGVKGLTLLDHEQVTEESCRAQFLVPVSARGQNRAQASLERAQNLNPMVEVHADPGRVED---KPDDFFLQFDAVCLTGCSRDLMVRVDQLCSQHRIKVFCGDVYGYYGYMFCNLG---QEYSYVEEKPKVVKP 183
BLAST of SMED30023745 vs. Ensembl Medaka
Match: uba1 (ubiquitin like modifier activating enzyme 1 [Source:NCBI gene;Acc:101158523]) HSP 1 Score: 69.3218 bits (168), Expect = 9.475e-12 Identity = 44/160 (27.50%), Postives = 76/160 (47.50%), Query Frame = -2
Query: 1515 YDRQIRLWGENGQIALENAKVCLIGSGCIGTEILKNLILPGIGSFTIVDDSVVTEEDLSNNFFVQKSDLNQSRAEVTARLLRELNEEVIVNYIEDSVESLMFRNPEYFLEFSVVIACGLNNKDLVDLDRLLYPENIGLIVCHSIGMVANLRVIIQEHPVV 1994
Y RQ+ + G ++N+ V + G +G E+ KN+IL G+ S T+ D V DLS+ F++++ DL ++RAE++ L ELN V V ++ +Y +F VV+ + + + I +I+ + G+ L E VV
Sbjct: 55 YSRQLYVLGHEAMKRMQNSNVLISGMRGLGVEVAKNVILGGVRSVTVHDQGVAEWRDLSSQFYLREEDLGKNRAEMSQLRLAELNNYVPVTAYTGALTE------DYLTKFQVVVLTNSTLDEQKHFGDFCHSKGIKIIIADTRGLFGQLFCDFGEEMVV 208 HSP 2 Score: 58.9214 bits (141), Expect = 1.716e-8 Identity = 41/165 (24.85%), Postives = 82/165 (49.70%), Query Frame = -2
Query: 1533 KTKRYDRQIRLWGENGQIALENAKVCLIGSGCIGTEILKNLILPGI----GSFTIVDDSVVTEEDLSNNFFVQKSDLNQSRAEVTARLLRELNEEVIVNYIEDSV--ESLMFRNPEYFLEFSVVIACGLNNKDL-VDLDRLLYPENIGLIVCHSIGMVANLRVII 2006
+ RYD QI ++G Q L + L+G+G IG E+LKN + G+ G + D + + +L+ F + SD+ + +++ A ++++N + + ++ V E+ + ++F V A L+N D + +DR L+ ++G N++V+I
Sbjct: 448 RNSRYDGQIAVFGTKLQDLLARQRYFLVGAGAIGCELLKNFAMIGLASGEGEVIVTDMDTIEKSNLNRQFLFRPSDVTKMKSDTAAAAVKQMNPSIRITGHQNRVGPETERVYDDDFFESLDGV-ANALDNVDARMYMDRRCVYYRKPLLESGTLGTKGNVQVVI 611
BLAST of SMED30023745 vs. Ensembl Medaka
Match: uba7 (ubiquitin-like modifier-activating enzyme 1 [Source:NCBI gene;Acc:101175149]) HSP 1 Score: 65.0846 bits (157), Expect = 1.944e-10 Identity = 42/150 (28.00%), Postives = 69/150 (46.00%), Query Frame = -2
Query: 1545 YDRQIRLWGENGQIALENAKVCLIGSGCIGTEILKNLILPGIGSFTIVDDSVVTEEDLSNNFFVQKSDLNQSRAEVTARLLRELNEEVIVNYIEDSVESLMFRNPEYFLEFSVVIACGLNNKDLVDLDRLLYPENIGLIVCHSIGMVANL 1994
Y RQ+ + G + A V + G +G EI KN+IL G+ + T+ D+ DLS+ F++ + L Q+RA + + L LN V V+ ++ E L F +V+ + D +L + I LIV + G+ L
Sbjct: 12 YSRQLYVLGHEAMRRMGAANVLIAGLKGLGVEIAKNVILSGVKAVTVQDEGSAVWSDLSSQFYLGECHLGQNRASCSLQQLSSLNPHVEVSAHTGPLDE------ELLLRFQIVVLTDSSLDDQKRFGQLCHSHGIKLIVADTKGLCGQL 155 HSP 2 Score: 62.3882 bits (150), Expect = 1.223e-9 Identity = 31/105 (29.52%), Postives = 60/105 (57.14%), Query Frame = -2
Query: 1698 RYDRQIRLWGENGQIALENAKVCLIGSGCIGTEILKNLILPGIGS-----FTIVDDSVVTEEDLSNNFFVQKSDLNQSRAEVTARLLRELNEEVIVNYIEDSVES 1997
RYD Q ++G + Q L N K L+G+G IG E+LKN L G+G+ T+ D + +L+ F + D+ ++++EV A+ + ++N ++ + ++ V++
Sbjct: 404 RYDGQTAVFGASFQKRLGNQKYFLVGAGAIGCELLKNFALIGLGAGEEGHITVTDMDYIERSNLNRQFLFRSPDIGKAKSEVAAKAVCQMNPQMKITSHQNRVDA 508
BLAST of SMED30023745 vs. Planmine SMEST
Match: SMESG000016314.1 (SMESG000016314.1) HSP 1 Score: 1056.59 bits (2731), Expect = 0.000e+0 Identity = 529/530 (99.81%), Postives = 530/530 (100.00%), Query Frame = -2
Query: 423 LEKTKRYDRQIRLWGENGQIALENAKVCLIGSGCIGTEILKNLILPGIGSFTIVDDSVVTEEDLSNNFFVQKSDLNQSRAEVTARLLRELNEEVIVNYIEDSVESLMFRNPEYFLEFSVVIACGLNNKDLVDLDRLLYPENIGLIVCHSIGMVANLRVIIQEHPVVEAKPDNIKPDLRLDKPLWEFVEFCDSQNLHEMSYKELAYCPWIIILFKYITSFQSKFAKWPDSYKDKQILRKMINEGRXXXXXXXXXXXXXXXSQYLDSFEEAVKSIPTTVFLSEIPINVRTLLEDSRCIDINRESKSFWILVAALKDFIRSEGNGSLPLRGEIPDMTADTGRYVAIQKIYRQASEKDIIHLEKHVYRICQTFQIPVTDELNRNSIARFARNCQHIQILSSYSLERELKTSPTHTEELVCPETDELSWYLALRAAVSFYTVERRWPGQRQIDSEIDVPHLRKLLQQDMNACVKFKDASVRDDFLLEMCRFSGVQLHSVCAFIAGIAAQEVIKLITGQYVPVMQPLMYNAIRQTT 2012
LEKTKRYDRQIRLWGENGQIALENAKVCLIGSGCIGTEILKNLILPGIGSFTIVDDSVVTEEDLSNNFFVQKSDLNQSRAEVTARLLRELNEEVIVNYIEDSVESLMFRNPEYFLEFSVVIACGLNNKDLVDLDRLLYPENIGLIVCHSIGMVANLRVIIQEHPVVEAKPDNIKPDLRLDKPLWEFVEFCDSQNLHEMSYKELAYCPWIIILFKYITSFQSKFAKWPDSYKDKQILRKMINEGREEYLKLCSSKEELSLSQYLDSFEEAVKSIPTTVFLSEIPINVRTLLEDSRCIDINRESKSFWILVAALKDFIRSEGNGSLPLRG+IPDMTADTGRYVAIQKIYRQASEKDIIHLEKHVYRICQTFQIPVTDELNRNSIARFARNCQHIQILSSYSLERELKTSPTHTEELVCPETDELSWYLALRAAVSFYTVERRWPGQRQIDSEIDVPHLRKLLQQDMNACVKFKDASVRDDFLLEMCRFSGVQLHSVCAFIAGIAAQEVIKLITGQYVPVMQPLMYNAIRQTT
Sbjct: 5 LEKTKRYDRQIRLWGENGQIALENAKVCLIGSGCIGTEILKNLILPGIGSFTIVDDSVVTEEDLSNNFFVQKSDLNQSRAEVTARLLRELNEEVIVNYIEDSVESLMFRNPEYFLEFSVVIACGLNNKDLVDLDRLLYPENIGLIVCHSIGMVANLRVIIQEHPVVEAKPDNIKPDLRLDKPLWEFVEFCDSQNLHEMSYKELAYCPWIIILFKYITSFQSKFAKWPDSYKDKQILRKMINEGREEYLKLCSSKEELSLSQYLDSFEEAVKSIPTTVFLSEIPINVRTLLEDSRCIDINRESKSFWILVAALKDFIRSEGNGSLPLRGDIPDMTADTGRYVAIQKIYRQASEKDIIHLEKHVYRICQTFQIPVTDELNRNSIARFARNCQHIQILSSYSLERELKTSPTHTEELVCPETDELSWYLALRAAVSFYTVERRWPGQRQIDSEIDVPHLRKLLQQDMNACVKFKDASVRDDFLLEMCRFSGVQLHSVCAFIAGIAAQEVIKLITGQYVPVMQPLMYNAIRQTT 534
BLAST of SMED30023745 vs. Planmine SMEST
Match: SMESG000036431.1 (SMESG000036431.1) HSP 1 Score: 86.2705 bits (212), Expect = 5.063e-18 Identity = 62/200 (31.00%), Postives = 93/200 (46.50%), Query Frame = -2
Query: 1443 EKTKRYDRQIRLWGENGQIALENAKVCLIGSGCIGTEILKNLILPGIGSFTIVDDSVVTEEDLSNNFFVQKSDLNQSRAEVTARLLRELNEEVIVNYIEDSVESLM--FRNPEYFLEFSVVIAC-----GLNNKDLVDLDRLLYPENIGLIVCHSIGMVANLRVIIQEHPVV----EAKPDNIKPDLRLDKPLWEFVEFC 2009
E+ + YDRQIRLWG + Q L+NAKV +IG + E++KNL+L GI + DD V EED++NNF +++S + ++RA + LN VIV + N EF +V+ C + ++L+ N S G + V + H V E + KP K + E +EFC
Sbjct: 7 EEEQVYDRQIRLWGLDAQNRLKNAKVLIIGINGLSCEVIKNLVLTGINKIALCDDLNVNEEDINNNFLIERSSIGENRAYAAKSRVENLNPRVIVEVYNHMPFEICDNINNKLTIEEFDIVVCCTSYIPSFKDNLWLNLNEKCRKYNKSFFGMDSFGWNGIMFVDLAHHEYVREEIEMQKPGEKPANNPSKWISEVLEFC 206
BLAST of SMED30023745 vs. Planmine SMEST
Match: SMESG000041261.1 (SMESG000041261.1) HSP 1 Score: 74.7146 bits (182), Expect = 1.358e-13 Identity = 42/150 (28.00%), Postives = 80/150 (53.33%), Query Frame = -2
Query: 1545 YDRQIRLWGENGQIALENAKVCLIGSGCIGTEILKNLILPGIGSFTIVDDSVVTEEDLSNNFFVQKSDLNQSRAEVTARLLRELNEEVIVNYIEDSVESLMFRNPEYFLEFSVVIACGLNNKDLVDLDRLLYPENIGLIVCHSIGMVANL 1994
Y RQ+ + G+ G + ++ V + G G +G EI KN++L G+ S T+ D V+E+DLS+ +F++K + Q+RA + L ELN V + IE + E +F++V+ + + +++R + NI + ++ G+ +
Sbjct: 14 YSRQLYVMGKEGMRNMASSSVLISGLGGLGVEIAKNIVLAGVKSVTLHDTKKVSEDDLSSQYFLRKEHIKQNRATASKLSLSELNSYVPIYVIERDL------TLETLKQFTIVVLTESSLDEQKNINRFCHENNIKFLNANTFGLFGQI 157 HSP 2 Score: 65.0846 bits (157), Expect = 1.417e-10 Identity = 46/167 (27.54%), Postives = 80/167 (47.90%), Query Frame = -2
Query: 1533 LEKTKRYDRQIRLWGENGQIALENAKVCLIGSGCIGTEILKNLILPGIGS-----FTIVDDSVVTEEDLSNNFFVQKSDLNQSRAEVTARLLRELNEEV-IVNYIEDSVESLMFRNPEYFLEFSVVIACGLNNKDL-VDLDRLLYPENIGLIVCHSIGMVANLRVII 2012
+ RY QI ++G Q L N ++GSG IG E LKN + GIGS + D + + +L+ F + D+ + ++EV AR ++ +N E+ I +++ E E F +A L+N + + +DRL L+ ++G N++VI+
Sbjct: 406 FHASNRYSGQIAIFGSEFQKKLSNLNYFVVGSGAIGCEHLKNFAMMGIGSGPRGKIVVTDMDSIEKSNLNRQFLFRSHDIQKMKSEVAARSIKTMNPEINIESHVNRVCEDTEHVYSEKFFNSIDGVANALDNVNARMYMDRLCVFYRKPLLESGTLGTKGNVQVIL 572
BLAST of SMED30023745 vs. Planmine SMEST
Match: SMESG000081278.1 (SMESG000081278.1) HSP 1 Score: 73.1738 bits (178), Expect = 4.361e-13 Identity = 40/150 (26.67%), Postives = 78/150 (52.00%), Query Frame = -2
Query: 1545 YDRQIRLWGENGQIALENAKVCLIGSGCIGTEILKNLILPGIGSFTIVDDSVVTEEDLSNNFFVQKSDLNQSRAEVTARLLRELNEEVIVNYIEDSVESLMFRNPEYFLEFSVVIACGLNNKDLVDLDRLLYPENIGLIVCHSIGMVANL 1994
Y RQ+ + G+ + + + + G G +G EI KN+IL G+ S T+ D+ TE LS+ +++ ++++ Q+RA + L ELN V VN + + E L F++V+ + +++++ + NI LI+ + G+ +
Sbjct: 48 YSRQLYVMGKEAMKNMSKSNILISGMGGLGVEIAKNVILAGVKSVTLHDELSATECSLSSQYYLNENNIGQNRATSCFKHLAELNSYVNVNVDTNPLTK------EMLLNFNIVVLTNIKQDEIINITGFCHLNNIKLIITRTFGLFGQI 191 HSP 2 Score: 56.6102 bits (135), Expect = 6.054e-8 Identity = 46/181 (25.41%), Postives = 85/181 (46.96%), Query Frame = -2
Query: 1533 NVVKLLEKTKRYDRQIRLWGENGQIALENAKVCLIGSGCIGTEILKNLILPGIGS-----------FTIVDDSVVTEEDLSNNFFVQKSDLNQSRAEVTARLLRELNEEVIVNYIEDSV--ESLMFRNPEYFLEFSVVIACGLNN---KDLVDLDRLLYPENIGLIVCHSIGMVANLRVII 2027
NV K RY QI ++G Q L K+ L+G+G IG E+LKN ++ G+G+ + D + +L+ F + D+ + ++ A + +N V + +E V E+ + N +F + SVV A L+N + +D + Y + L+ ++G N++V++
Sbjct: 444 NVPHFDNKLNRYKSQIEVFGLKFQEKLSKLKIFLVGAGAIGCELLKNFVMMGVGASINDSDDDSGKIVVTDMDSIERSNLNRQFLFRSKDIGKMKSLTAAEAAKCMNPYVRIEALELKVGKETESYFNDAFFNDISVV-ANALDNVEARQYMDRRCVFYLK--PLLESGTLGTKCNVQVVL 621
BLAST of SMED30023745 vs. Planmine SMEST
Match: SMESG000070068.1 (SMESG000070068.1) HSP 1 Score: 63.1586 bits (152), Expect = 5.417e-10 Identity = 31/97 (31.96%), Postives = 56/97 (57.73%), Query Frame = -2
Query: 1704 YDRQIRLWGENGQIALENAKVCLIGSGCIGTEILKNLILPGIGSFTIVDDSVVTEEDLSNNFFVQKSDLNQSRAEVTARLLRELNEEVIVNYIEDSV 1994
Y RQ+ + G+ + ++ + G +G E+ KN+IL G+ T+ D +T DLS+ F+ SD+ ++RAEV L E+NE V V+++++ +
Sbjct: 10 YSRQLYVLGKKAMQEISRKRILITGINGLGLEVAKNVILSGVSQVTLHDTQSITSSDLSSYFYGNISDIGRNRAEVCHHHLCEINEYVKVDWLKEQL 106 HSP 2 Score: 62.3882 bits (150), Expect = 8.975e-10 Identity = 36/123 (29.27%), Postives = 64/123 (52.03%), Query Frame = -2
Query: 1650 RYDRQIRLWGENGQIALENAKVCLIGSGCIGTEILKNLILPGIGSFT-----IVDDSVVTEEDLSNNFFVQKSDLNQSRAEVTARLLRELNEEVIVNYIEDSV--ESLMFRNPEYFLEFSVVI 1997
RYD QI ++G Q LEN K LIG+G IG E+LKN + G+G+ + D + +L+ F + D+ QS++ + + ++N+ + + E+ + E+ F N ++F V+
Sbjct: 409 RYDGQIAVFGSQFQKKLENLKYFLIGAGAIGCEMLKNFAMIGVGTGADGKIFVTDMDFIERSNLNRQFLFRNWDIGQSKSLTATKAVLKMNQSLHIEAHENRLGQETEDFYNNKFFRSLDCVV 531 The following BLAST results are available for this feature:
BLAST of SMED30023745 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of SMED30023745 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 5
BLAST of SMED30023745 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 5
BLAST of SMED30023745 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of SMED30023745 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of SMED30023745 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of SMED30023745 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of SMED30023745 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of SMED30023745 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of SMED30023745 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 3
BLAST of SMED30023745 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 5
BLAST of SMED30023745 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 5
BLAST of SMED30023745 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of SMED30023745 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30023745 ID=SMED30023745|Name=SMED30023745|organism=Schmidtea mediterranea sexual|type=transcript|length=2052bpback to top protein sequence of SMED30023745-orf-1 >SMED30023745-orf-1 ID=SMED30023745-orf-1|Name=SMED30023745-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=430bp MFRNPEYFLEFSVVIACGLNNKDLVDLDRLLYPENIGLIVCHSIGMVANLback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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