Peptidase M60 domain-containing protein
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny AppSub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30023730 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 4
Homology
BLAST of Peptidase M60 domain-containing protein vs. TrEMBL
Match: A0A1S6KMG4 (Peptidase M60 domain-containing protein OS=Schmidtea mediterranea OX=79327 PE=2 SV=1) HSP 1 Score: 164.081 bits (414), Expect = 2.657e-44 Identity = 100/123 (81.30%), Postives = 111/123 (90.24%), Query Frame = 2 Query: 2 MSQYLGWGFFKDVFEFYETNKISPITDSQKMITFMXXXXXXXXXXXXXMFDFWGFPIDESLRQQLSKLPCFFPADDFLVSFSLIRKNYISNKYPECNXXXXXXXXXXXXXXINGMRPNFIQEI 370 MSQYLGWGF+KD+FE+Y+TN ISP TDS+KMITFMNI+SIISQQN+IS+FDFWGFPID +L QQLS LPCFFPADDFLV+ S RKNYISNKYP+CNSKSSFYKDY FINGMRPNFIQEI Sbjct: 799 MSQYLGWGFYKDLFEYYDTNNISPSTDSEKMITFMNITSIISQQNMISIFDFWGFPIDNNLSQQLSTLPCFFPADDFLVALSPKRKNYISNKYPQCNSKSSFYKDYSPDLFINGMRPNFIQEI 921
BLAST of Peptidase M60 domain-containing protein vs. TrEMBL
Match: A0A1S6KMG0 (Peptidase M60 domain-containing protein OS=Schmidtea mediterranea OX=79327 PE=2 SV=1) HSP 1 Score: 68.1662 bits (165), Expect = 1.076e-10 Identity = 49/123 (39.84%), Postives = 79/123 (64.23%), Query Frame = 2 Query: 2 MSQYLGWGFFKDVFEFYETN-KISPITDSQKMITFMXXXXXXXXXXXXXMFDFWGFPIDESLRQQLSKLPCFFPADDFLVSFSLIRKNYISNKYPECNXXXXXXXXXXXXXXINGMRPNFIQE 367 M+ + G+GFF F++Y + ++ +D +K + N++S I+ QNL+ +FD W PI + +R+ LSK CF P DDF + S R+ +I KYP+C+ KS+FY+D++ S G RPNFI++ Sbjct: 808 MANHFGFGFFNKAFKYYLNHPELKADSDEEKYDLWANVTSTITNQNLMPIFDLWNIPIGDEMRKYLSKFQCFLP-DDFFLKSSRSREEFILKKYPDCHRKSTFYEDFNPESLTQGHRPNFIKK 929
BLAST of Peptidase M60 domain-containing protein vs. TrEMBL
Match: R7UHN9 (Peptidase M60 domain-containing protein OS=Capitella teleta OX=283909 GN=CAPTEDRAFT_195748 PE=4 SV=1) HSP 1 Score: 57.3806 bits (137), Expect = 5.510e-7 Identity = 30/78 (38.46%), Postives = 48/78 (61.54%), Query Frame = 2 Query: 2 MSQYLGWGFFKDVFEFYE--TNKISPITDSQKMITFMXXXXXXXXXXXXXMFDFWGFPIDESLRQQLSKLPCFFPADD 229 ++ +LGWG +K VF YE +N+ P TD +K+ ++ + QNL ++F FWGF I L++ L++LP F P D+ Sbjct: 600 LAHHLGWGTYKAVFRKYEVMSNEQKPKTDDEKIRLWITTFAECCGQNLCALFRFWGFTIPNDLQRNLAELPLFLPMDE 677
BLAST of Peptidase M60 domain-containing protein vs. Planmine SMEST
Match: SMESG000042170.1 (SMESG000042170.1) HSP 1 Score: 193.356 bits (490), Expect = 2.534e-58 Identity = 123/123 (100.00%), Postives = 123/123 (100.00%), Query Frame = 2 Query: 2 MSQYLGWGFFKDVFEFYETNKISPITDSQKMITFMXXXXXXXXXXXXXMFDFWGFPIDESLRQQLSKLPCFFPADDFLVSFSLIRKNYISNKYPECNXXXXXXXXXXXXXXINGMRPNFIQEI 370 MSQYLGWGFFKDVFEFYETNKISPITDSQKMITFMNISSIISQQNLISMFDFWGFPIDESLRQQLSKLPCFFPADDFLVSFSLIRKNYISNKYPECNSKSSFYKDYDSSSFINGMRPNFIQEI Sbjct: 800 MSQYLGWGFFKDVFEFYETNKISPITDSQKMITFMNISSIISQQNLISMFDFWGFPIDESLRQQLSKLPCFFPADDFLVSFSLIRKNYISNKYPECNSKSSFYKDYDSSSFINGMRPNFIQEI 922
BLAST of Peptidase M60 domain-containing protein vs. Planmine SMEST
Match: SMESG000042170.1 (SMESG000042170.1) HSP 1 Score: 193.356 bits (490), Expect = 2.601e-58 Identity = 123/123 (100.00%), Postives = 123/123 (100.00%), Query Frame = 2 Query: 2 MSQYLGWGFFKDVFEFYETNKISPITDSQKMITFMXXXXXXXXXXXXXMFDFWGFPIDESLRQQLSKLPCFFPADDFLVSFSLIRKNYISNKYPECNXXXXXXXXXXXXXXINGMRPNFIQEI 370 MSQYLGWGFFKDVFEFYETNKISPITDSQKMITFMNISSIISQQNLISMFDFWGFPIDESLRQQLSKLPCFFPADDFLVSFSLIRKNYISNKYPECNSKSSFYKDYDSSSFINGMRPNFIQEI Sbjct: 797 MSQYLGWGFFKDVFEFYETNKISPITDSQKMITFMNISSIISQQNLISMFDFWGFPIDESLRQQLSKLPCFFPADDFLVSFSLIRKNYISNKYPECNSKSSFYKDYDSSSFINGMRPNFIQEI 919
BLAST of Peptidase M60 domain-containing protein vs. Planmine SMEST
Match: SMESG000042174.1 (SMESG000042174.1) HSP 1 Score: 191.815 bits (486), Expect = 1.196e-57 Identity = 122/123 (99.19%), Postives = 122/123 (99.19%), Query Frame = 2 Query: 2 MSQYLGWGFFKDVFEFYETNKISPITDSQKMITFMXXXXXXXXXXXXXMFDFWGFPIDESLRQQLSKLPCFFPADDFLVSFSLIRKNYISNKYPECNXXXXXXXXXXXXXXINGMRPNFIQEI 370 MSQYLGWGFFKDVFEFYETNKISPITDSQKMITFMNISSIISQQNLISMFDFWGFPIDESLRQQLSKLPCFFPADDFLVS SLIRKNYISNKYPECNSKSSFYKDYDSSSFINGMRPNFIQEI Sbjct: 800 MSQYLGWGFFKDVFEFYETNKISPITDSQKMITFMNISSIISQQNLISMFDFWGFPIDESLRQQLSKLPCFFPADDFLVSLSLIRKNYISNKYPECNSKSSFYKDYDSSSFINGMRPNFIQEI 922
BLAST of Peptidase M60 domain-containing protein vs. Planmine SMEST
Match: SMESG000042174.1 (SMESG000042174.1) HSP 1 Score: 191.43 bits (485), Expect = 1.241e-57 Identity = 122/123 (99.19%), Postives = 122/123 (99.19%), Query Frame = 2 Query: 2 MSQYLGWGFFKDVFEFYETNKISPITDSQKMITFMXXXXXXXXXXXXXMFDFWGFPIDESLRQQLSKLPCFFPADDFLVSFSLIRKNYISNKYPECNXXXXXXXXXXXXXXINGMRPNFIQEI 370 MSQYLGWGFFKDVFEFYETNKISPITDSQKMITFMNISSIISQQNLISMFDFWGFPIDESLRQQLSKLPCFFPADDFLVS SLIRKNYISNKYPECNSKSSFYKDYDSSSFINGMRPNFIQEI Sbjct: 797 MSQYLGWGFFKDVFEFYETNKISPITDSQKMITFMNISSIISQQNLISMFDFWGFPIDESLRQQLSKLPCFFPADDFLVSLSLIRKNYISNKYPECNSKSSFYKDYDSSSFINGMRPNFIQEI 919
BLAST of Peptidase M60 domain-containing protein vs. Planmine SMEST
Match: SMESG000042235.1 (SMESG000042235.1) HSP 1 Score: 179.489 bits (454), Expect = 2.241e-53 Identity = 113/123 (91.87%), Postives = 117/123 (95.12%), Query Frame = 2 Query: 2 MSQYLGWGFFKDVFEFYETNKISPITDSQKMITFMXXXXXXXXXXXXXMFDFWGFPIDESLRQQLSKLPCFFPADDFLVSFSLIRKNYISNKYPECNXXXXXXXXXXXXXXINGMRPNFIQEI 370 MSQYLGWGFFKDVFEFYETNKISPITDSQKMITFMNISSIISQQNLISMFDFWGFPIDESL QQLS LPCFFPADDFLVS SLIRK+YISNKYP+CNSK+SFYKDYD + FI GMRPNFIQEI Sbjct: 737 MSQYLGWGFFKDVFEFYETNKISPITDSQKMITFMNISSIISQQNLISMFDFWGFPIDESLSQQLSTLPCFFPADDFLVSLSLIRKSYISNKYPKCNSKTSFYKDYDPNPFIKGMRPNFIQEI 859 The following BLAST results are available for this feature:
BLAST of Peptidase M60 domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of Peptidase M60 domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of Peptidase M60 domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of Peptidase M60 domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of Peptidase M60 domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of Peptidase M60 domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of Peptidase M60 domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of Peptidase M60 domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 3
BLAST of Peptidase M60 domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of Peptidase M60 domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of Peptidase M60 domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of Peptidase M60 domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of Peptidase M60 domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of Peptidase M60 domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30023730 ID=SMED30023730|Name=Peptidase M60 domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=381bpback to top protein sequence of SMED30023730-orf-1 >SMED30023730-orf-1 ID=SMED30023730-orf-1|Name=SMED30023730-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=124bp MSQYLGWGFFKDVFEFYETNKISPITDSQKMITFMNISSIISQQNLISMFback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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