Midasin
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30023713 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 4
Homology
BLAST of Midasin vs. Ensembl Human
Match: MDN1 (midasin AAA ATPase 1 [Source:HGNC Symbol;Acc:HGNC:18302]) HSP 1 Score: 329.331 bits (843), Expect = 1.183e-107 Identity = 182/376 (48.40%), Postives = 234/376 (62.23%), Query Frame = 2
Query: 89 VVTIRDLLSWVNFINISIGNKTSLYQ--------SIFHGAYMVIIDSINDKLNKS-FYEELLIKK--LDYLVKSSFPDIVECLDFQSSRF---------ELSVKENKVGIDPFYIELGSHSDWXXXXXXXXXXKNFQYSFNSKTTGSNVYRLFRALQVSKKPLLLEGSPGVGKTSLVTQLAKLVGFKVVRINLSEQTDANDLFGCFLPNENGKAGELSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPEINQKFMVEPGKTRLFACQNPVSEGSGRKGLPKSFVNRFTQVYMSSLSDSDYRNILNCLYPAIARETIGFIVEFVQNLREHVVVMKKFGTKGSPWEFNLRDLCRWCDLMVKHQ 1156
VV+IRD+LSWVNF+N +G + +L + S H A +V ID I + S F LL +K L +L+K IV ++Q + E + +N GI PF+I G + + + Y+ ++ TT N RL RA ++ KKP+LLEGSPGVGKTSLV LAK G +VRINLSEQTD DLFG LP E GK GE +WRDGPLL ALKAG W++LDE+NLASQSVLE LNAC DHRGEI++PE+ F V+ KT++F CQNP +G GRKGLP+SF+NRFTQV++ L+ D I + L+PAI + + +V F + V V KK+G KG PWEFNLRDL RWC LM+ Q
Sbjct: 1599 VVSIRDILSWVNFMN-KMGEEAALKRPEIISTVTSFVHAACLVYIDGIGSGVTSSGFGTALLARKECLKFLIKR-LAKIVRLTEYQKNELKIYDRMKAKEFTGIDNLWGIHPFFIPRGP---------VLHRNNIADYALSAGTTAMNAQRLLRATKL-KKPILLEGSPGVGKTSLVGALAKASGNTLVRINLSEQTDITDLFGADLPVEGGKGGEFAWRDGPLLAALKAGHWVVLDELNLASQSVLEGLNACFDHRGEIYVPELGMSFQVQHEKTKIFGCQNPFRQGGGRKGLPRSFLNRFTQVFVDPLTVIDMEFIASTLFPAIEKNIVKKMVAFNNQIDHEVTVEKKWGQKGGPWEFNLRDLFRWCQLMLVDQ 1962 HSP 2 Score: 85.1149 bits (209), Expect = 1.183e-107 Identity = 51/135 (37.78%), Postives = 77/135 (57.04%), Query Frame = 1
Query: 1159 RTKEDQDACGKVYNELADKSRFARFIPSGLDFYITDQVLKIG--QTQLSVNIANNTDNSKQFNKH--LLLAGQLAILESMMLTVDMGWLTVLVGPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGSFEQ 1551
RT+ED+ V+ ++ S + Y+ ++ +I QL ++ + ++H LLL LES+M V M W+ +LVGPA GKT++V+LL+ +TG+ LK M+MNSAMDTT+LLG FEQ
Sbjct: 1982 RTEEDKKKVIAVFKDV---------FGSNSNPYMGTRLFRITPYDVQLGYSVLSRGSCVPHPSRHPLLLLHQSFQPLESIMKCVQMSWMVILVGPASVGKTSLVQLLAHLTGHTLKIMAMNSAMDTTELLGGFEQ 2107 HSP 3 Score: 234.572 bits (597), Expect = 2.176e-60 Identity = 127/283 (44.88%), Postives = 183/283 (64.66%), Query Frame = 1
Query: 9145 WSQYESKTEYLSRQLCEMLRIVLEPTKANKLGGAYRTGKRLNMKKVIPYIASGFRKDKIWLRRNKPSQREYQVIIGVDNSKSMAYNVNHQLFFESLSIVLNGLTFLEVGQVGLCKFGAKAEVVLPLYEKFSSEIGDRLIETFDFSDKCSKFIPILEQSIKLFEENQNFLSSSAVRXXXXXXXXXXXXXXXXXXXXPKFISLIREAHNKGIFVVVILLDHADSKDSILNLKMVIDDG----CDFVRFLDVFPLQNYTVLQDVTKLPTLLTDVLRQWFELSSSAD 9981
W Y T LS++LCE LR++LEPT+A KL G YRTGKRLN++KVIPYIAS FRKDKIWLRR KPS+R+YQ+ + +D+S SM N QL FESL+++ N LT LEVGQ+ +C FG +++ P +E+FS G +++ F K +K LE +F Q LS + +T+QLLL++SDG + ++ ++ A N IFV+ ++LD+ S+DSIL++K+ I G + +++ FP Y +L+DV LP L+D LRQWFEL +++D
Sbjct: 5313 WQSYLILTAPLSQRLCEELRLILEPTQAAKLKGDYRTGKRLNIRKVIPYIASQFRKDKIWLRRTKPSKRQYQICLAIDDSSSMVDNHTKQLAFESLAVIGNALTLLEVGQIAVCSFGESVKLLHPFHEQFSDYSGSQILRLCKFQQKKTKIAQFLESVANMFAAAQQ-LSQNISSETAQLLLVVSDGRGLFLEGKERVLAAVQAARNANIFVIFVVLDNPSSRDSILDIKVPIFKGPGEMPEIRSYMEEFPFPYYIILRDVNALPETLSDALRQWFELVTASD 5594 HSP 4 Score: 135.961 bits (341), Expect = 1.231e-30 Identity = 82/177 (46.33%), Postives = 109/177 (61.58%), Query Frame = 1
Query: 1564 GKFKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGLVYGDRIEEIKPHKNFRLFLILDPDCGGEISKAMRNRGLEICIIPENRELTLTDNDLLAILITIYKLSVKNADSLLRFHKTMILAQSETPQIKIG----NINSLIQAAKLI----KDGYSLDQSL 2070
G F+WVDS LVQAL+ GDWL++D VNFC+ SVLDRLN LLEP G L ++ERG++ G I P+ NFRLFL +DP G+IS+AMRNRGLEI I E T + DL +L ++ + D LL H +ET +G ++++LIQ A LI + G SLD++
Sbjct: 2220 GTFEWVDSMLVQALKSGDWLLMDNVNFCNPSVLDRLNALLEPGGVLTISERGMIDGS-TPTITPNPNFRLFLSMDP-VHGDISRAMRNRGLEIYISGEGDASTPDNLDLKVLLHSLGLVGNSVCDILLALH-------TETRSTVVGSPTSSVSTLIQTAILIVQYLQRGLSLDRAF 2387 HSP 5 Score: 127.872 bits (320), Expect = 4.086e-28 Identity = 78/226 (34.51%), Postives = 115/226 (50.88%), Query Frame = 2
Query: 470 NVYRLFRALQVSKKPLLLEGSPGVGKTSLVTQLAKLVGFKVVRINLSEQTDANDLFGCFLPNENGKAGELSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPEINQKFMVEPGKTRLFACQNPVSEGSGRKGLPKSFVNRFTQVYMSSLSDSDYRNILN---CLYPAIARETIGFIVEFVQNLREHVVVMKKFGTKGSPWEFNLRDLCRWCD 1138
N+ + R + P+L++G VGKTSL+ LA G VRIN E TD + GC+ + +GK L +++G L+ A++ G WI+LDE+NLA VLE LN LD E+ + E + P + LFA QNP GRK L ++F NRF +++ L S+ IL+ L P+ + + +++ R V K G LRDL RW +
Sbjct: 1065 NLRDIVRVVSAGTYPVLIQGETSVGKTSLIQWLAAATGNHCVRINNHEHTDIQEYIGCYTSDSSGK---LVFKEGVLIDAMRKGYWIILDELNLAPTDVLEALNRLLDDNRELLVTETQEVVKAHP-RFMLFATQNPPGLYGGRKVLSRAFRNRFVELHFDELPSSELETILHKRCSLPPSYCSKLVKVMLDLQSYRRSSSVFAGKQGF------ITLRDLFRWAE 1280 HSP 6 Score: 118.627 bits (296), Expect = 2.500e-25 Identity = 85/347 (24.50%), Postives = 173/347 (49.86%), Query Frame = 1
Query: 5191 EEKQDTTVFRDCYRFAAALISS--DNQGIPSDDCKLFAANLSALNWEYETLSPGVPQNTIGRP---HDIYQDPIDVDNLLQAQKILPNANVSVTKLLGIYPEHTTXXXXXXXXXXXXXXSGDYSLIVFINNVERLIHEIQQWEVVAARHVSLIQHVDELNGLLVSWRHQELTMWENTISTANWQLERAVSREFFNHYNLI---VQESNKIQYDNDFESINLILEPILNSLLNSTVGNFEYRLKTMKLSIESLISNREIDYRFKDRLKNILLNHYDFYRLYLGNVQEHLSSEYKRIGKELRDFIKTKRWGKTP--PKITDVLKCHKIVFKYKRQWSGIINQPCTKFFV 6201
E K ++F CY+ A+L++ G+ +D +L + L A + TL P + + +P +D YQ P +V Q Q +L + +V+ LL +PEH L + ++ I + + F+N +E L+ + Q WE A+R +SL +H+D ++ +++ WR EL W ++ + ++ +F+ Y ++ +QE + Q D+ ++ L++ + + S++G F RL+ + + ++ +++ KD L ++L N Y +Y+ + VQ + + KEL++F+K +W V K H+ +FK+ +++ ++++PC V
Sbjct: 3648 EAKHYLSLFLSCYQTGASLVTHFYPLMGVELND-RLLGSQLLACTLSHNTLFGEAPSDLMVKPDGPYDFYQHP-NVPEARQCQPVLQGFSEAVSHLLQDWPEHPALEQLLVVMDRIRSFPLSSPISKFLNGLEILLAKAQDWEENASRALSLRKHLDLISQMIIRWRKLELNCWSMSLDNTMKRHTEKSTKHWFSIYQMLEKHMQEQTEEQEDDKQMTLMLLVSTLQAFIEGSSLGEFHVRLQMLLVFHCHVLLMPQVEG--KDSLCSVLWNLYHYYKQFFDRVQAKIVELRSPLEKELKEFVKISKWNDVSFWSIKQSVEKTHRTLFKFMKKFEAVLSEPCRSSLV 3990 HSP 7 Score: 88.1965 bits (217), Expect = 4.024e-16 Identity = 62/176 (35.23%), Postives = 93/176 (52.84%), Query Frame = 1
Query: 1393 TVDMGWLTVLVGPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGSFE--QQKEGGK--------FKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGLV--YGDRIEEIKPHKNFRLFLILDPDCGG-----EISKAMRNRGLEI-CIIPENRE 1866
++ G +LVG G GKTTI ++ + + L S+S + M+T+D LG +QK K F+W D LVQA+++ + +LD ++ SVL+RLN +LE SL+L E+G IE + K FR+ ++P GG E+S A+RNR EI C +RE
Sbjct: 1379 ALEFGEPVLLVGDTGCGKTTICQVFAALANQKLYSVSCHLHMETSDFLGGLRPVRQKPNDKEEIDTSRLFEWHDGPLVQAMKEDGFFLLDEISLADDSVLERLNSVLEVEKSLVLAEKGSPEDKDSEIELLTAGKKFRILATMNP--GGDFGKKELSPALRNRFTEIWCPQSTSRE 1552 HSP 8 Score: 85.1149 bits (209), Expect = 2.978e-15 Identity = 52/152 (34.21%), Postives = 82/152 (53.95%), Query Frame = 2
Query: 689 DGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGE--IFIPEINQKFMVEPGKTRLFACQNPVSEGSGRKGLPKSFVNRFTQVYMSSLSDSDYRNILNCLY---PAIARETIGFIVEFVQNLREHVVVMKKFGTKGSPWEFNLRDLCR 1129
+G L QA+K G+WILLDE+NLA+ +LECL+ L+ + + + + +V RLFAC NP ++ G++ LP NRFT++Y+ L + +L Y ++ + T+ I+ F LR+ GT P ++LR LCR
Sbjct: 820 EGTLAQAVKKGEWILLDEINLAAPEILECLSGLLEGSSGSLVLLDRGDTEPLVRHPDFRLFACMNPATD-VGKRNLPPGIRNRFTELYVEELESKEDLQVLIVDYLKGLSVNKNTVQGIINFYTALRKESGTKLVDGTGHRP-HYSLRTLCR 969 HSP 9 Score: 79.7221 bits (195), Expect = 1.280e-13 Identity = 53/161 (32.92%), Postives = 83/161 (51.55%), Query Frame = 1
Query: 1375 LESMMLTVDMGWLTVLV-GPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGSFEQQKEGGKFKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGLVYGDRIEEIKPHKNFRLFLILDPD--CGGE--ISKAMRNRGLEI 1842
L ++ V G VL+ G GKT++++ L+ TGNH ++ + D + +G + GK + + L+ A+R+G W+ILD +N VL+ LN LL+ N LL+TE E +K H F LF +P GG +S+A RNR +E+
Sbjct: 1066 LRDIVRVVSAGTYPVLIQGETSVGKTSLIQWLAAATGNHCVRINNHEHTDIQEYIGCYTSDS-SGKLVFKEGVLIDAMRKGYWIILDELNLAPTDVLEALNRLLDDNRELLVTETQ-------EVVKAHPRFMLFATQNPPGLYGGRKVLSRAFRNRFVEL 1218 HSP 10 Score: 79.337 bits (194), Expect = 1.897e-13 Identity = 50/166 (30.12%), Postives = 88/166 (53.01%), Query Frame = 1
Query: 1417 VLVGPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGS--FEQQKEGGKFKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGLVYGDRIEEIKPHKNFRLFLILDP--DCGGE--ISKAMRNRGLEICIIPENRELTLTDNDLLA 1896
+L G G GKT++V L+ +GN L ++++ D TDL G+ + +GG+F W D L+ AL+ G W++LD +N S SVL+ LN + G + + E G+ + + H+ ++F +P GG + ++ NR ++ + P LT+ D + +A
Sbjct: 1750 LLEGSPGVGKTSLVGALAKASGNTLVRINLSEQTDITDLFGADLPVEGGKGGEFAWRDGPLLAALKAGHWVVLDELNLASQSVLEGLNACFDHRGEIYVPELGMSFQVQ------HEKTKIFGCQNPFRQGGGRKGLPRSFLNRFTQVFVDP----LTVIDMEFIA 1905 HSP 11 Score: 77.0258 bits (188), Expect = 7.876e-13 Identity = 66/241 (27.39%), Postives = 112/241 (46.47%), Query Frame = 2
Query: 500 VSKKPLLLEGSPGVGKTSLVTQLAKLVGF----KVVRINLSEQTDANDLFGCFLPNENGKAGELSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPEINQKFMVEPGKTRLFACQNPVSEGSGRKGLP----KSFVNRF-TQVYMSSLSDSDYRNILNCLYPAIARETIGFIVEFVQ--NLREHVVVMKKFGTKGSP-----------------WEFNLRDLCRWCD 1138
S+ +LLEG G GKTSLV LA + G +++++ L +QTD+ L G + + GE W+ G L QA G WILL++++ A V+ L L++ GE+ IP V PG + FA + +S G G P + ++++ T++++ +L + +L YP++ + ++Q + H G + +P E +LRDL WC+
Sbjct: 320 ASQNAVLLEGPIGCGKTSLVEYLAAVTGRTKPPQLLKVQLGDQTDSKMLLGMYRCTD--VPGEFVWQPGTLTQAATMGHWILLEDIDYAPLDVVSVLIPLLEN-GELLIPGRGDCLKVAPG-FQFFATRRLLSCG-GNWYRPLNSHATLLDKYWTKIHLDNLDKRELNEVLQSRYPSLLAVVDHLLDIYIQLTGEKHHSWSDSSVGCEQAPEEVSEARRENKRPTLEGRELSLRDLLNWCN 555 HSP 12 Score: 75.0998 bits (183), Expect = 3.439e-12 Identity = 71/259 (27.41%), Postives = 114/259 (44.02%), Query Frame = 2
Query: 488 RALQVSKKPLLLEGSPGVGKTSLVTQLAKLVGFKVVRINLSEQTDANDLFGCFLP--------NENGKAGELSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPEINQ--------KFMVEPGKTRLFACQNPVSEGSGRKGLPKSFVNRFTQVYM-SSLSDSDYRNIL-NCLYPAIARETIG--------FIVEFVQNLREHVVVMKKFGTKGSPWEFNLRDLCRWCDLMVKHQHEPRKTRTPV 1186
RAL+ + P+LL G G GKT++ A L K+ ++ + +D G P E + W DGPL+QA+K + LLDE++LA SVLE LN+ L+ + + E + + K R+ A NP + G+K L + NRFT+++ S S D I+ + L P + I +++F+ L ++FG K ++RD+ W + M K E R +
Sbjct: 1378 RALEFGE-PVLLVGDTGCGKTTICQVFAALANQKLYSVSCHLHMETSDFLGGLRPVRQKPNDKEEIDTSRLFEWHDGPLVQAMKEDGFFLLDEISLADDSVLERLNSVLEVEKSLVLAEKGSPEDKDSEIELLTAGKKFRILATMNPGGD-FGKKELSPALRNRFTEIWCPQSTSREDLIQIISHNLRPGLCLGRIDPKGSDIPEVMLDFIDWL-----THQEFGRKCV---VSIRDILSWVNFMNKMGEEAALKRPEI 1626 HSP 13 Score: 72.4034 bits (176), Expect = 2.064e-11 Identity = 84/328 (25.61%), Postives = 150/328 (45.73%), Query Frame = 1
Query: 7063 KSYLVNMKHMECLSQQKLIIKGINELIEIVKNRFSPENKKINSSITESLLKFNDSNERAIIEIET-SLHVANDNFMDTDDSFD-GFVN----ELAVVIREKISAFKTI------LLTDNVDKS--------FIKVNT-HINALEQ--------SLDTQKIL----DLFTKIKSFFCDLNMNQVNKFRNLINEMV--LPKLRSLEKVEKEFLGLLLNYHRVLISATDALLTVTTKLMKEGFCIPNDSAWNNEAENSNPSEKMNDFEMAGMDGGVGTNDVTDEIESESQIE-----GNNKDKKDETENQPDIPDEDDGIEMTDDFNGKMH 7926
K + V + CLSQ + ++G+ L + E + ++ ESL +A+ + T H+ + + D GFV ++ + IR + A + + +N D++ F ++ + H+ L + +L QKI+ +L ++KS+ D + F + +V +P L S + FL + L HR L V T+L ++GFC+P + ++ E + + +D+E G+ G G DV+D+I +E Q+E G KDK+D +++ DI ED+ IEM++DF+GKMH
Sbjct: 4417 KDFEVCSSALSCLSQVSVHLQGLESLF--ILPGMEVEQRDSQMALVESLEYVRGEISKAMADFTTWKTHLLTSDSQGGNQMLDEGFVEDFSEQMEIAIRAILCAIQNLEERKNEKAEENTDQASPQEDYAGFERLQSGHLTKLLEDDFWADVSTLHVQKIISAISELLERLKSYGEDGTAAKHLFFSQSCSLLVRLVPVLSSYSDLVLFFLTMSLATHRSTAKLLSVLAQVFTELAQKGFCLPKEFMEDSAGEGAT---EFHDYEGGGIGEGEGMKDVSDQIGNEEQVEDTFQKGQEKDKED-PDSKSDIKGEDNAIEMSEDFDGKMH 4738 HSP 14 Score: 66.6254 bits (161), Expect = 1.130e-9 Identity = 45/158 (28.48%), Postives = 76/158 (48.10%), Query Frame = 1
Query: 1270 VLKIGQTQLSVNIANNTDNSKQ----FNKHLLLAGQLAILESMMLTVDMGWLTVLVGPAGTGKTTIVKLLSDITGN----HLKSMSMNSAMDTTDLLGSFEQQKEGGKFKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERG 1719
V+ GQ + N +S++ ++L+ L+++ + V +L GP G GKT++V+ L+ +TG L + + D+ LLG + G+F W TL QA G W++L+ +++ V+ L LLE NG LL+ RG
Sbjct: 273 VVLPGQLPAPGELGGNRSSSREQELALRSYVLVESVCKSLQTLAMAVASQNAVLLEGPIGCGKTSLVEYLAAVTGRTKPPQLLKVQLGDQTDSKMLLGMYRCTDVPGEFVWQPGTLTQAATMGHWILLEDIDYAPLDVVSVLIPLLE-NGELLIPGRG 429 HSP 15 Score: 64.3142 bits (155), Expect = 6.397e-9 Identity = 36/97 (37.11%), Postives = 60/97 (61.86%), Query Frame = 1
Query: 1570 FKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLE-PNGSLLLTERGLVYGDRIEEIKPHKNFRLFLILDP--DCGGE-ISKAMRNRGLEICI 1848
F +V+ TL QA+++G+W++LD +N + +L+ L+GLLE +GSL+L +RG E + H +FRLF ++P D G + +RNR E+ +
Sbjct: 816 FAFVEGTLAQAVKKGEWILLDEINLAAPEILECLSGLLEGSSGSLVLLDRG-----DTEPLVRHPDFRLFACMNPATDVGKRNLPPGIRNRFTELYV 907 HSP 16 Score: 55.4546 bits (132), Expect = 2.865e-6 Identity = 37/111 (33.33%), Postives = 56/111 (50.45%), Query Frame = 2
Query: 671 GELSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPEINQKFMVEPGKT-----RLFACQNPVSEGSGRKGLPKSFVNRFTQVYMSSLSDSDYRNILN 988
G W D L+QALK+GDW+L+D +N + SVL+ LNA L+ G + I E P T RLF +PV + ++ NR ++Y+S D+ + L+
Sbjct: 2220 GTFEWVDSMLVQALKSGDWLLMDNVNFCNPSVLDRLNALLEPGGVLTISERGMIDGSTPTITPNPNFRLFLSMDPV-----HGDISRAMRNRGLEIYISGEGDASTPDNLD 2325 HSP 17 Score: 53.5286 bits (127), Expect = 9.978e-6 Identity = 24/59 (40.68%), Postives = 39/59 (66.10%), Query Frame = 1
Query: 1372 ILESMMLTVDMGWLTVLVGPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGSFE 1548
++E + + V G +LVG GTGKT+ ++ L+ ITG+ L+ ++MN DT DLLG ++
Sbjct: 659 LIEQLAVCVSKGEPVLLVGETGTGKTSTIQYLAHITGHRLRVVNMNQQSDTADLLGGYK 717
BLAST of Midasin vs. Ensembl Human
Match: MDN1 (midasin AAA ATPase 1 [Source:HGNC Symbol;Acc:HGNC:18302]) HSP 1 Score: 329.331 bits (843), Expect = 1.183e-107 Identity = 182/376 (48.40%), Postives = 234/376 (62.23%), Query Frame = 2
Query: 89 VVTIRDLLSWVNFINISIGNKTSLYQ--------SIFHGAYMVIIDSINDKLNKS-FYEELLIKK--LDYLVKSSFPDIVECLDFQSSRF---------ELSVKENKVGIDPFYIELGSHSDWXXXXXXXXXXKNFQYSFNSKTTGSNVYRLFRALQVSKKPLLLEGSPGVGKTSLVTQLAKLVGFKVVRINLSEQTDANDLFGCFLPNENGKAGELSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPEINQKFMVEPGKTRLFACQNPVSEGSGRKGLPKSFVNRFTQVYMSSLSDSDYRNILNCLYPAIARETIGFIVEFVQNLREHVVVMKKFGTKGSPWEFNLRDLCRWCDLMVKHQ 1156
VV+IRD+LSWVNF+N +G + +L + S H A +V ID I + S F LL +K L +L+K IV ++Q + E + +N GI PF+I G + + + Y+ ++ TT N RL RA ++ KKP+LLEGSPGVGKTSLV LAK G +VRINLSEQTD DLFG LP E GK GE +WRDGPLL ALKAG W++LDE+NLASQSVLE LNAC DHRGEI++PE+ F V+ KT++F CQNP +G GRKGLP+SF+NRFTQV++ L+ D I + L+PAI + + +V F + V V KK+G KG PWEFNLRDL RWC LM+ Q
Sbjct: 1599 VVSIRDILSWVNFMN-KMGEEAALKRPEIISTVTSFVHAACLVYIDGIGSGVTSSGFGTALLARKECLKFLIKR-LAKIVRLTEYQKNELKIYDRMKAKEFTGIDNLWGIHPFFIPRGP---------VLHRNNIADYALSAGTTAMNAQRLLRATKL-KKPILLEGSPGVGKTSLVGALAKASGNTLVRINLSEQTDITDLFGADLPVEGGKGGEFAWRDGPLLAALKAGHWVVLDELNLASQSVLEGLNACFDHRGEIYVPELGMSFQVQHEKTKIFGCQNPFRQGGGRKGLPRSFLNRFTQVFVDPLTVIDMEFIASTLFPAIEKNIVKKMVAFNNQIDHEVTVEKKWGQKGGPWEFNLRDLFRWCQLMLVDQ 1962 HSP 2 Score: 85.1149 bits (209), Expect = 1.183e-107 Identity = 51/135 (37.78%), Postives = 77/135 (57.04%), Query Frame = 1
Query: 1159 RTKEDQDACGKVYNELADKSRFARFIPSGLDFYITDQVLKIG--QTQLSVNIANNTDNSKQFNKH--LLLAGQLAILESMMLTVDMGWLTVLVGPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGSFEQ 1551
RT+ED+ V+ ++ S + Y+ ++ +I QL ++ + ++H LLL LES+M V M W+ +LVGPA GKT++V+LL+ +TG+ LK M+MNSAMDTT+LLG FEQ
Sbjct: 1982 RTEEDKKKVIAVFKDV---------FGSNSNPYMGTRLFRITPYDVQLGYSVLSRGSCVPHPSRHPLLLLHQSFQPLESIMKCVQMSWMVILVGPASVGKTSLVQLLAHLTGHTLKIMAMNSAMDTTELLGGFEQ 2107 HSP 3 Score: 234.572 bits (597), Expect = 2.176e-60 Identity = 127/283 (44.88%), Postives = 183/283 (64.66%), Query Frame = 1
Query: 9145 WSQYESKTEYLSRQLCEMLRIVLEPTKANKLGGAYRTGKRLNMKKVIPYIASGFRKDKIWLRRNKPSQREYQVIIGVDNSKSMAYNVNHQLFFESLSIVLNGLTFLEVGQVGLCKFGAKAEVVLPLYEKFSSEIGDRLIETFDFSDKCSKFIPILEQSIKLFEENQNFLSSSAVRXXXXXXXXXXXXXXXXXXXXPKFISLIREAHNKGIFVVVILLDHADSKDSILNLKMVIDDG----CDFVRFLDVFPLQNYTVLQDVTKLPTLLTDVLRQWFELSSSAD 9981
W Y T LS++LCE LR++LEPT+A KL G YRTGKRLN++KVIPYIAS FRKDKIWLRR KPS+R+YQ+ + +D+S SM N QL FESL+++ N LT LEVGQ+ +C FG +++ P +E+FS G +++ F K +K LE +F Q LS + +T+QLLL++SDG + ++ ++ A N IFV+ ++LD+ S+DSIL++K+ I G + +++ FP Y +L+DV LP L+D LRQWFEL +++D
Sbjct: 5313 WQSYLILTAPLSQRLCEELRLILEPTQAAKLKGDYRTGKRLNIRKVIPYIASQFRKDKIWLRRTKPSKRQYQICLAIDDSSSMVDNHTKQLAFESLAVIGNALTLLEVGQIAVCSFGESVKLLHPFHEQFSDYSGSQILRLCKFQQKKTKIAQFLESVANMFAAAQQ-LSQNISSETAQLLLVVSDGRGLFLEGKERVLAAVQAARNANIFVIFVVLDNPSSRDSILDIKVPIFKGPGEMPEIRSYMEEFPFPYYIILRDVNALPETLSDALRQWFELVTASD 5594 HSP 4 Score: 135.961 bits (341), Expect = 1.231e-30 Identity = 82/177 (46.33%), Postives = 109/177 (61.58%), Query Frame = 1
Query: 1564 GKFKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGLVYGDRIEEIKPHKNFRLFLILDPDCGGEISKAMRNRGLEICIIPENRELTLTDNDLLAILITIYKLSVKNADSLLRFHKTMILAQSETPQIKIG----NINSLIQAAKLI----KDGYSLDQSL 2070
G F+WVDS LVQAL+ GDWL++D VNFC+ SVLDRLN LLEP G L ++ERG++ G I P+ NFRLFL +DP G+IS+AMRNRGLEI I E T + DL +L ++ + D LL H +ET +G ++++LIQ A LI + G SLD++
Sbjct: 2220 GTFEWVDSMLVQALKSGDWLLMDNVNFCNPSVLDRLNALLEPGGVLTISERGMIDGS-TPTITPNPNFRLFLSMDP-VHGDISRAMRNRGLEIYISGEGDASTPDNLDLKVLLHSLGLVGNSVCDILLALH-------TETRSTVVGSPTSSVSTLIQTAILIVQYLQRGLSLDRAF 2387 HSP 5 Score: 127.872 bits (320), Expect = 4.086e-28 Identity = 78/226 (34.51%), Postives = 115/226 (50.88%), Query Frame = 2
Query: 470 NVYRLFRALQVSKKPLLLEGSPGVGKTSLVTQLAKLVGFKVVRINLSEQTDANDLFGCFLPNENGKAGELSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPEINQKFMVEPGKTRLFACQNPVSEGSGRKGLPKSFVNRFTQVYMSSLSDSDYRNILN---CLYPAIARETIGFIVEFVQNLREHVVVMKKFGTKGSPWEFNLRDLCRWCD 1138
N+ + R + P+L++G VGKTSL+ LA G VRIN E TD + GC+ + +GK L +++G L+ A++ G WI+LDE+NLA VLE LN LD E+ + E + P + LFA QNP GRK L ++F NRF +++ L S+ IL+ L P+ + + +++ R V K G LRDL RW +
Sbjct: 1065 NLRDIVRVVSAGTYPVLIQGETSVGKTSLIQWLAAATGNHCVRINNHEHTDIQEYIGCYTSDSSGK---LVFKEGVLIDAMRKGYWIILDELNLAPTDVLEALNRLLDDNRELLVTETQEVVKAHP-RFMLFATQNPPGLYGGRKVLSRAFRNRFVELHFDELPSSELETILHKRCSLPPSYCSKLVKVMLDLQSYRRSSSVFAGKQGF------ITLRDLFRWAE 1280 HSP 6 Score: 118.627 bits (296), Expect = 2.500e-25 Identity = 85/347 (24.50%), Postives = 173/347 (49.86%), Query Frame = 1
Query: 5191 EEKQDTTVFRDCYRFAAALISS--DNQGIPSDDCKLFAANLSALNWEYETLSPGVPQNTIGRP---HDIYQDPIDVDNLLQAQKILPNANVSVTKLLGIYPEHTTXXXXXXXXXXXXXXSGDYSLIVFINNVERLIHEIQQWEVVAARHVSLIQHVDELNGLLVSWRHQELTMWENTISTANWQLERAVSREFFNHYNLI---VQESNKIQYDNDFESINLILEPILNSLLNSTVGNFEYRLKTMKLSIESLISNREIDYRFKDRLKNILLNHYDFYRLYLGNVQEHLSSEYKRIGKELRDFIKTKRWGKTP--PKITDVLKCHKIVFKYKRQWSGIINQPCTKFFV 6201
E K ++F CY+ A+L++ G+ +D +L + L A + TL P + + +P +D YQ P +V Q Q +L + +V+ LL +PEH L + ++ I + + F+N +E L+ + Q WE A+R +SL +H+D ++ +++ WR EL W ++ + ++ +F+ Y ++ +QE + Q D+ ++ L++ + + S++G F RL+ + + ++ +++ KD L ++L N Y +Y+ + VQ + + KEL++F+K +W V K H+ +FK+ +++ ++++PC V
Sbjct: 3648 EAKHYLSLFLSCYQTGASLVTHFYPLMGVELND-RLLGSQLLACTLSHNTLFGEAPSDLMVKPDGPYDFYQHP-NVPEARQCQPVLQGFSEAVSHLLQDWPEHPALEQLLVVMDRIRSFPLSSPISKFLNGLEILLAKAQDWEENASRALSLRKHLDLISQMIIRWRKLELNCWSMSLDNTMKRHTEKSTKHWFSIYQMLEKHMQEQTEEQEDDKQMTLMLLVSTLQAFIEGSSLGEFHVRLQMLLVFHCHVLLMPQVEG--KDSLCSVLWNLYHYYKQFFDRVQAKIVELRSPLEKELKEFVKISKWNDVSFWSIKQSVEKTHRTLFKFMKKFEAVLSEPCRSSLV 3990 HSP 7 Score: 88.1965 bits (217), Expect = 4.024e-16 Identity = 62/176 (35.23%), Postives = 93/176 (52.84%), Query Frame = 1
Query: 1393 TVDMGWLTVLVGPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGSFE--QQKEGGK--------FKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGLV--YGDRIEEIKPHKNFRLFLILDPDCGG-----EISKAMRNRGLEI-CIIPENRE 1866
++ G +LVG G GKTTI ++ + + L S+S + M+T+D LG +QK K F+W D LVQA+++ + +LD ++ SVL+RLN +LE SL+L E+G IE + K FR+ ++P GG E+S A+RNR EI C +RE
Sbjct: 1379 ALEFGEPVLLVGDTGCGKTTICQVFAALANQKLYSVSCHLHMETSDFLGGLRPVRQKPNDKEEIDTSRLFEWHDGPLVQAMKEDGFFLLDEISLADDSVLERLNSVLEVEKSLVLAEKGSPEDKDSEIELLTAGKKFRILATMNP--GGDFGKKELSPALRNRFTEIWCPQSTSRE 1552 HSP 8 Score: 85.1149 bits (209), Expect = 2.978e-15 Identity = 52/152 (34.21%), Postives = 82/152 (53.95%), Query Frame = 2
Query: 689 DGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGE--IFIPEINQKFMVEPGKTRLFACQNPVSEGSGRKGLPKSFVNRFTQVYMSSLSDSDYRNILNCLY---PAIARETIGFIVEFVQNLREHVVVMKKFGTKGSPWEFNLRDLCR 1129
+G L QA+K G+WILLDE+NLA+ +LECL+ L+ + + + + +V RLFAC NP ++ G++ LP NRFT++Y+ L + +L Y ++ + T+ I+ F LR+ GT P ++LR LCR
Sbjct: 820 EGTLAQAVKKGEWILLDEINLAAPEILECLSGLLEGSSGSLVLLDRGDTEPLVRHPDFRLFACMNPATD-VGKRNLPPGIRNRFTELYVEELESKEDLQVLIVDYLKGLSVNKNTVQGIINFYTALRKESGTKLVDGTGHRP-HYSLRTLCR 969 HSP 9 Score: 79.7221 bits (195), Expect = 1.280e-13 Identity = 53/161 (32.92%), Postives = 83/161 (51.55%), Query Frame = 1
Query: 1375 LESMMLTVDMGWLTVLV-GPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGSFEQQKEGGKFKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGLVYGDRIEEIKPHKNFRLFLILDPD--CGGE--ISKAMRNRGLEI 1842
L ++ V G VL+ G GKT++++ L+ TGNH ++ + D + +G + GK + + L+ A+R+G W+ILD +N VL+ LN LL+ N LL+TE E +K H F LF +P GG +S+A RNR +E+
Sbjct: 1066 LRDIVRVVSAGTYPVLIQGETSVGKTSLIQWLAAATGNHCVRINNHEHTDIQEYIGCYTSDS-SGKLVFKEGVLIDAMRKGYWIILDELNLAPTDVLEALNRLLDDNRELLVTETQ-------EVVKAHPRFMLFATQNPPGLYGGRKVLSRAFRNRFVEL 1218 HSP 10 Score: 79.337 bits (194), Expect = 1.897e-13 Identity = 50/166 (30.12%), Postives = 88/166 (53.01%), Query Frame = 1
Query: 1417 VLVGPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGS--FEQQKEGGKFKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGLVYGDRIEEIKPHKNFRLFLILDP--DCGGE--ISKAMRNRGLEICIIPENRELTLTDNDLLA 1896
+L G G GKT++V L+ +GN L ++++ D TDL G+ + +GG+F W D L+ AL+ G W++LD +N S SVL+ LN + G + + E G+ + + H+ ++F +P GG + ++ NR ++ + P LT+ D + +A
Sbjct: 1750 LLEGSPGVGKTSLVGALAKASGNTLVRINLSEQTDITDLFGADLPVEGGKGGEFAWRDGPLLAALKAGHWVVLDELNLASQSVLEGLNACFDHRGEIYVPELGMSFQVQ------HEKTKIFGCQNPFRQGGGRKGLPRSFLNRFTQVFVDP----LTVIDMEFIA 1905 HSP 11 Score: 77.0258 bits (188), Expect = 7.876e-13 Identity = 66/241 (27.39%), Postives = 112/241 (46.47%), Query Frame = 2
Query: 500 VSKKPLLLEGSPGVGKTSLVTQLAKLVGF----KVVRINLSEQTDANDLFGCFLPNENGKAGELSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPEINQKFMVEPGKTRLFACQNPVSEGSGRKGLP----KSFVNRF-TQVYMSSLSDSDYRNILNCLYPAIARETIGFIVEFVQ--NLREHVVVMKKFGTKGSP-----------------WEFNLRDLCRWCD 1138
S+ +LLEG G GKTSLV LA + G +++++ L +QTD+ L G + + GE W+ G L QA G WILL++++ A V+ L L++ GE+ IP V PG + FA + +S G G P + ++++ T++++ +L + +L YP++ + ++Q + H G + +P E +LRDL WC+
Sbjct: 320 ASQNAVLLEGPIGCGKTSLVEYLAAVTGRTKPPQLLKVQLGDQTDSKMLLGMYRCTD--VPGEFVWQPGTLTQAATMGHWILLEDIDYAPLDVVSVLIPLLEN-GELLIPGRGDCLKVAPG-FQFFATRRLLSCG-GNWYRPLNSHATLLDKYWTKIHLDNLDKRELNEVLQSRYPSLLAVVDHLLDIYIQLTGEKHHSWSDSSVGCEQAPEEVSEARRENKRPTLEGRELSLRDLLNWCN 555 HSP 12 Score: 75.0998 bits (183), Expect = 3.439e-12 Identity = 71/259 (27.41%), Postives = 114/259 (44.02%), Query Frame = 2
Query: 488 RALQVSKKPLLLEGSPGVGKTSLVTQLAKLVGFKVVRINLSEQTDANDLFGCFLP--------NENGKAGELSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPEINQ--------KFMVEPGKTRLFACQNPVSEGSGRKGLPKSFVNRFTQVYM-SSLSDSDYRNIL-NCLYPAIARETIG--------FIVEFVQNLREHVVVMKKFGTKGSPWEFNLRDLCRWCDLMVKHQHEPRKTRTPV 1186
RAL+ + P+LL G G GKT++ A L K+ ++ + +D G P E + W DGPL+QA+K + LLDE++LA SVLE LN+ L+ + + E + + K R+ A NP + G+K L + NRFT+++ S S D I+ + L P + I +++F+ L ++FG K ++RD+ W + M K E R +
Sbjct: 1378 RALEFGE-PVLLVGDTGCGKTTICQVFAALANQKLYSVSCHLHMETSDFLGGLRPVRQKPNDKEEIDTSRLFEWHDGPLVQAMKEDGFFLLDEISLADDSVLERLNSVLEVEKSLVLAEKGSPEDKDSEIELLTAGKKFRILATMNPGGD-FGKKELSPALRNRFTEIWCPQSTSREDLIQIISHNLRPGLCLGRIDPKGSDIPEVMLDFIDWL-----THQEFGRKCV---VSIRDILSWVNFMNKMGEEAALKRPEI 1626 HSP 13 Score: 72.4034 bits (176), Expect = 2.064e-11 Identity = 84/328 (25.61%), Postives = 150/328 (45.73%), Query Frame = 1
Query: 7063 KSYLVNMKHMECLSQQKLIIKGINELIEIVKNRFSPENKKINSSITESLLKFNDSNERAIIEIET-SLHVANDNFMDTDDSFD-GFVN----ELAVVIREKISAFKTI------LLTDNVDKS--------FIKVNT-HINALEQ--------SLDTQKIL----DLFTKIKSFFCDLNMNQVNKFRNLINEMV--LPKLRSLEKVEKEFLGLLLNYHRVLISATDALLTVTTKLMKEGFCIPNDSAWNNEAENSNPSEKMNDFEMAGMDGGVGTNDVTDEIESESQIE-----GNNKDKKDETENQPDIPDEDDGIEMTDDFNGKMH 7926
K + V + CLSQ + ++G+ L + E + ++ ESL +A+ + T H+ + + D GFV ++ + IR + A + + +N D++ F ++ + H+ L + +L QKI+ +L ++KS+ D + F + +V +P L S + FL + L HR L V T+L ++GFC+P + ++ E + + +D+E G+ G G DV+D+I +E Q+E G KDK+D +++ DI ED+ IEM++DF+GKMH
Sbjct: 4417 KDFEVCSSALSCLSQVSVHLQGLESLF--ILPGMEVEQRDSQMALVESLEYVRGEISKAMADFTTWKTHLLTSDSQGGNQMLDEGFVEDFSEQMEIAIRAILCAIQNLEERKNEKAEENTDQASPQEDYAGFERLQSGHLTKLLEDDFWADVSTLHVQKIISAISELLERLKSYGEDGTAAKHLFFSQSCSLLVRLVPVLSSYSDLVLFFLTMSLATHRSTAKLLSVLAQVFTELAQKGFCLPKEFMEDSAGEGAT---EFHDYEGGGIGEGEGMKDVSDQIGNEEQVEDTFQKGQEKDKED-PDSKSDIKGEDNAIEMSEDFDGKMH 4738 HSP 14 Score: 66.6254 bits (161), Expect = 1.130e-9 Identity = 45/158 (28.48%), Postives = 76/158 (48.10%), Query Frame = 1
Query: 1270 VLKIGQTQLSVNIANNTDNSKQ----FNKHLLLAGQLAILESMMLTVDMGWLTVLVGPAGTGKTTIVKLLSDITGN----HLKSMSMNSAMDTTDLLGSFEQQKEGGKFKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERG 1719
V+ GQ + N +S++ ++L+ L+++ + V +L GP G GKT++V+ L+ +TG L + + D+ LLG + G+F W TL QA G W++L+ +++ V+ L LLE NG LL+ RG
Sbjct: 273 VVLPGQLPAPGELGGNRSSSREQELALRSYVLVESVCKSLQTLAMAVASQNAVLLEGPIGCGKTSLVEYLAAVTGRTKPPQLLKVQLGDQTDSKMLLGMYRCTDVPGEFVWQPGTLTQAATMGHWILLEDIDYAPLDVVSVLIPLLE-NGELLIPGRG 429 HSP 15 Score: 64.3142 bits (155), Expect = 6.397e-9 Identity = 36/97 (37.11%), Postives = 60/97 (61.86%), Query Frame = 1
Query: 1570 FKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLE-PNGSLLLTERGLVYGDRIEEIKPHKNFRLFLILDP--DCGGE-ISKAMRNRGLEICI 1848
F +V+ TL QA+++G+W++LD +N + +L+ L+GLLE +GSL+L +RG E + H +FRLF ++P D G + +RNR E+ +
Sbjct: 816 FAFVEGTLAQAVKKGEWILLDEINLAAPEILECLSGLLEGSSGSLVLLDRG-----DTEPLVRHPDFRLFACMNPATDVGKRNLPPGIRNRFTELYV 907 HSP 16 Score: 55.4546 bits (132), Expect = 2.865e-6 Identity = 37/111 (33.33%), Postives = 56/111 (50.45%), Query Frame = 2
Query: 671 GELSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPEINQKFMVEPGKT-----RLFACQNPVSEGSGRKGLPKSFVNRFTQVYMSSLSDSDYRNILN 988
G W D L+QALK+GDW+L+D +N + SVL+ LNA L+ G + I E P T RLF +PV + ++ NR ++Y+S D+ + L+
Sbjct: 2220 GTFEWVDSMLVQALKSGDWLLMDNVNFCNPSVLDRLNALLEPGGVLTISERGMIDGSTPTITPNPNFRLFLSMDPV-----HGDISRAMRNRGLEIYISGEGDASTPDNLD 2325 HSP 17 Score: 53.5286 bits (127), Expect = 9.978e-6 Identity = 24/59 (40.68%), Postives = 39/59 (66.10%), Query Frame = 1
Query: 1372 ILESMMLTVDMGWLTVLVGPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGSFE 1548
++E + + V G +LVG GTGKT+ ++ L+ ITG+ L+ ++MN DT DLLG ++
Sbjct: 659 LIEQLAVCVSKGEPVLLVGETGTGKTSTIQYLAHITGHRLRVVNMNQQSDTADLLGGYK 717
BLAST of Midasin vs. Ensembl Human
Match: MDN1 (midasin AAA ATPase 1 [Source:HGNC Symbol;Acc:HGNC:18302]) HSP 1 Score: 329.331 bits (843), Expect = 1.183e-107 Identity = 182/376 (48.40%), Postives = 234/376 (62.23%), Query Frame = 2
Query: 89 VVTIRDLLSWVNFINISIGNKTSLYQ--------SIFHGAYMVIIDSINDKLNKS-FYEELLIKK--LDYLVKSSFPDIVECLDFQSSRF---------ELSVKENKVGIDPFYIELGSHSDWXXXXXXXXXXKNFQYSFNSKTTGSNVYRLFRALQVSKKPLLLEGSPGVGKTSLVTQLAKLVGFKVVRINLSEQTDANDLFGCFLPNENGKAGELSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPEINQKFMVEPGKTRLFACQNPVSEGSGRKGLPKSFVNRFTQVYMSSLSDSDYRNILNCLYPAIARETIGFIVEFVQNLREHVVVMKKFGTKGSPWEFNLRDLCRWCDLMVKHQ 1156
VV+IRD+LSWVNF+N +G + +L + S H A +V ID I + S F LL +K L +L+K IV ++Q + E + +N GI PF+I G + + + Y+ ++ TT N RL RA ++ KKP+LLEGSPGVGKTSLV LAK G +VRINLSEQTD DLFG LP E GK GE +WRDGPLL ALKAG W++LDE+NLASQSVLE LNAC DHRGEI++PE+ F V+ KT++F CQNP +G GRKGLP+SF+NRFTQV++ L+ D I + L+PAI + + +V F + V V KK+G KG PWEFNLRDL RWC LM+ Q
Sbjct: 1599 VVSIRDILSWVNFMN-KMGEEAALKRPEIISTVTSFVHAACLVYIDGIGSGVTSSGFGTALLARKECLKFLIKR-LAKIVRLTEYQKNELKIYDRMKAKEFTGIDNLWGIHPFFIPRGP---------VLHRNNIADYALSAGTTAMNAQRLLRATKL-KKPILLEGSPGVGKTSLVGALAKASGNTLVRINLSEQTDITDLFGADLPVEGGKGGEFAWRDGPLLAALKAGHWVVLDELNLASQSVLEGLNACFDHRGEIYVPELGMSFQVQHEKTKIFGCQNPFRQGGGRKGLPRSFLNRFTQVFVDPLTVIDMEFIASTLFPAIEKNIVKKMVAFNNQIDHEVTVEKKWGQKGGPWEFNLRDLFRWCQLMLVDQ 1962 HSP 2 Score: 85.1149 bits (209), Expect = 1.183e-107 Identity = 51/135 (37.78%), Postives = 77/135 (57.04%), Query Frame = 1
Query: 1159 RTKEDQDACGKVYNELADKSRFARFIPSGLDFYITDQVLKIG--QTQLSVNIANNTDNSKQFNKH--LLLAGQLAILESMMLTVDMGWLTVLVGPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGSFEQ 1551
RT+ED+ V+ ++ S + Y+ ++ +I QL ++ + ++H LLL LES+M V M W+ +LVGPA GKT++V+LL+ +TG+ LK M+MNSAMDTT+LLG FEQ
Sbjct: 1982 RTEEDKKKVIAVFKDV---------FGSNSNPYMGTRLFRITPYDVQLGYSVLSRGSCVPHPSRHPLLLLHQSFQPLESIMKCVQMSWMVILVGPASVGKTSLVQLLAHLTGHTLKIMAMNSAMDTTELLGGFEQ 2107 HSP 3 Score: 234.572 bits (597), Expect = 2.176e-60 Identity = 127/283 (44.88%), Postives = 183/283 (64.66%), Query Frame = 1
Query: 9145 WSQYESKTEYLSRQLCEMLRIVLEPTKANKLGGAYRTGKRLNMKKVIPYIASGFRKDKIWLRRNKPSQREYQVIIGVDNSKSMAYNVNHQLFFESLSIVLNGLTFLEVGQVGLCKFGAKAEVVLPLYEKFSSEIGDRLIETFDFSDKCSKFIPILEQSIKLFEENQNFLSSSAVRXXXXXXXXXXXXXXXXXXXXPKFISLIREAHNKGIFVVVILLDHADSKDSILNLKMVIDDG----CDFVRFLDVFPLQNYTVLQDVTKLPTLLTDVLRQWFELSSSAD 9981
W Y T LS++LCE LR++LEPT+A KL G YRTGKRLN++KVIPYIAS FRKDKIWLRR KPS+R+YQ+ + +D+S SM N QL FESL+++ N LT LEVGQ+ +C FG +++ P +E+FS G +++ F K +K LE +F Q LS + +T+QLLL++SDG + ++ ++ A N IFV+ ++LD+ S+DSIL++K+ I G + +++ FP Y +L+DV LP L+D LRQWFEL +++D
Sbjct: 5313 WQSYLILTAPLSQRLCEELRLILEPTQAAKLKGDYRTGKRLNIRKVIPYIASQFRKDKIWLRRTKPSKRQYQICLAIDDSSSMVDNHTKQLAFESLAVIGNALTLLEVGQIAVCSFGESVKLLHPFHEQFSDYSGSQILRLCKFQQKKTKIAQFLESVANMFAAAQQ-LSQNISSETAQLLLVVSDGRGLFLEGKERVLAAVQAARNANIFVIFVVLDNPSSRDSILDIKVPIFKGPGEMPEIRSYMEEFPFPYYIILRDVNALPETLSDALRQWFELVTASD 5594 HSP 4 Score: 135.961 bits (341), Expect = 1.231e-30 Identity = 82/177 (46.33%), Postives = 109/177 (61.58%), Query Frame = 1
Query: 1564 GKFKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGLVYGDRIEEIKPHKNFRLFLILDPDCGGEISKAMRNRGLEICIIPENRELTLTDNDLLAILITIYKLSVKNADSLLRFHKTMILAQSETPQIKIG----NINSLIQAAKLI----KDGYSLDQSL 2070
G F+WVDS LVQAL+ GDWL++D VNFC+ SVLDRLN LLEP G L ++ERG++ G I P+ NFRLFL +DP G+IS+AMRNRGLEI I E T + DL +L ++ + D LL H +ET +G ++++LIQ A LI + G SLD++
Sbjct: 2220 GTFEWVDSMLVQALKSGDWLLMDNVNFCNPSVLDRLNALLEPGGVLTISERGMIDGS-TPTITPNPNFRLFLSMDP-VHGDISRAMRNRGLEIYISGEGDASTPDNLDLKVLLHSLGLVGNSVCDILLALH-------TETRSTVVGSPTSSVSTLIQTAILIVQYLQRGLSLDRAF 2387 HSP 5 Score: 127.872 bits (320), Expect = 4.086e-28 Identity = 78/226 (34.51%), Postives = 115/226 (50.88%), Query Frame = 2
Query: 470 NVYRLFRALQVSKKPLLLEGSPGVGKTSLVTQLAKLVGFKVVRINLSEQTDANDLFGCFLPNENGKAGELSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPEINQKFMVEPGKTRLFACQNPVSEGSGRKGLPKSFVNRFTQVYMSSLSDSDYRNILN---CLYPAIARETIGFIVEFVQNLREHVVVMKKFGTKGSPWEFNLRDLCRWCD 1138
N+ + R + P+L++G VGKTSL+ LA G VRIN E TD + GC+ + +GK L +++G L+ A++ G WI+LDE+NLA VLE LN LD E+ + E + P + LFA QNP GRK L ++F NRF +++ L S+ IL+ L P+ + + +++ R V K G LRDL RW +
Sbjct: 1065 NLRDIVRVVSAGTYPVLIQGETSVGKTSLIQWLAAATGNHCVRINNHEHTDIQEYIGCYTSDSSGK---LVFKEGVLIDAMRKGYWIILDELNLAPTDVLEALNRLLDDNRELLVTETQEVVKAHP-RFMLFATQNPPGLYGGRKVLSRAFRNRFVELHFDELPSSELETILHKRCSLPPSYCSKLVKVMLDLQSYRRSSSVFAGKQGF------ITLRDLFRWAE 1280 HSP 6 Score: 118.627 bits (296), Expect = 2.500e-25 Identity = 85/347 (24.50%), Postives = 173/347 (49.86%), Query Frame = 1
Query: 5191 EEKQDTTVFRDCYRFAAALISS--DNQGIPSDDCKLFAANLSALNWEYETLSPGVPQNTIGRP---HDIYQDPIDVDNLLQAQKILPNANVSVTKLLGIYPEHTTXXXXXXXXXXXXXXSGDYSLIVFINNVERLIHEIQQWEVVAARHVSLIQHVDELNGLLVSWRHQELTMWENTISTANWQLERAVSREFFNHYNLI---VQESNKIQYDNDFESINLILEPILNSLLNSTVGNFEYRLKTMKLSIESLISNREIDYRFKDRLKNILLNHYDFYRLYLGNVQEHLSSEYKRIGKELRDFIKTKRWGKTP--PKITDVLKCHKIVFKYKRQWSGIINQPCTKFFV 6201
E K ++F CY+ A+L++ G+ +D +L + L A + TL P + + +P +D YQ P +V Q Q +L + +V+ LL +PEH L + ++ I + + F+N +E L+ + Q WE A+R +SL +H+D ++ +++ WR EL W ++ + ++ +F+ Y ++ +QE + Q D+ ++ L++ + + S++G F RL+ + + ++ +++ KD L ++L N Y +Y+ + VQ + + KEL++F+K +W V K H+ +FK+ +++ ++++PC V
Sbjct: 3648 EAKHYLSLFLSCYQTGASLVTHFYPLMGVELND-RLLGSQLLACTLSHNTLFGEAPSDLMVKPDGPYDFYQHP-NVPEARQCQPVLQGFSEAVSHLLQDWPEHPALEQLLVVMDRIRSFPLSSPISKFLNGLEILLAKAQDWEENASRALSLRKHLDLISQMIIRWRKLELNCWSMSLDNTMKRHTEKSTKHWFSIYQMLEKHMQEQTEEQEDDKQMTLMLLVSTLQAFIEGSSLGEFHVRLQMLLVFHCHVLLMPQVEG--KDSLCSVLWNLYHYYKQFFDRVQAKIVELRSPLEKELKEFVKISKWNDVSFWSIKQSVEKTHRTLFKFMKKFEAVLSEPCRSSLV 3990 HSP 7 Score: 88.1965 bits (217), Expect = 4.024e-16 Identity = 62/176 (35.23%), Postives = 93/176 (52.84%), Query Frame = 1
Query: 1393 TVDMGWLTVLVGPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGSFE--QQKEGGK--------FKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGLV--YGDRIEEIKPHKNFRLFLILDPDCGG-----EISKAMRNRGLEI-CIIPENRE 1866
++ G +LVG G GKTTI ++ + + L S+S + M+T+D LG +QK K F+W D LVQA+++ + +LD ++ SVL+RLN +LE SL+L E+G IE + K FR+ ++P GG E+S A+RNR EI C +RE
Sbjct: 1379 ALEFGEPVLLVGDTGCGKTTICQVFAALANQKLYSVSCHLHMETSDFLGGLRPVRQKPNDKEEIDTSRLFEWHDGPLVQAMKEDGFFLLDEISLADDSVLERLNSVLEVEKSLVLAEKGSPEDKDSEIELLTAGKKFRILATMNP--GGDFGKKELSPALRNRFTEIWCPQSTSRE 1552 HSP 8 Score: 85.1149 bits (209), Expect = 2.978e-15 Identity = 52/152 (34.21%), Postives = 82/152 (53.95%), Query Frame = 2
Query: 689 DGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGE--IFIPEINQKFMVEPGKTRLFACQNPVSEGSGRKGLPKSFVNRFTQVYMSSLSDSDYRNILNCLY---PAIARETIGFIVEFVQNLREHVVVMKKFGTKGSPWEFNLRDLCR 1129
+G L QA+K G+WILLDE+NLA+ +LECL+ L+ + + + + +V RLFAC NP ++ G++ LP NRFT++Y+ L + +L Y ++ + T+ I+ F LR+ GT P ++LR LCR
Sbjct: 820 EGTLAQAVKKGEWILLDEINLAAPEILECLSGLLEGSSGSLVLLDRGDTEPLVRHPDFRLFACMNPATD-VGKRNLPPGIRNRFTELYVEELESKEDLQVLIVDYLKGLSVNKNTVQGIINFYTALRKESGTKLVDGTGHRP-HYSLRTLCR 969 HSP 9 Score: 79.7221 bits (195), Expect = 1.280e-13 Identity = 53/161 (32.92%), Postives = 83/161 (51.55%), Query Frame = 1
Query: 1375 LESMMLTVDMGWLTVLV-GPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGSFEQQKEGGKFKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGLVYGDRIEEIKPHKNFRLFLILDPD--CGGE--ISKAMRNRGLEI 1842
L ++ V G VL+ G GKT++++ L+ TGNH ++ + D + +G + GK + + L+ A+R+G W+ILD +N VL+ LN LL+ N LL+TE E +K H F LF +P GG +S+A RNR +E+
Sbjct: 1066 LRDIVRVVSAGTYPVLIQGETSVGKTSLIQWLAAATGNHCVRINNHEHTDIQEYIGCYTSDS-SGKLVFKEGVLIDAMRKGYWIILDELNLAPTDVLEALNRLLDDNRELLVTETQ-------EVVKAHPRFMLFATQNPPGLYGGRKVLSRAFRNRFVEL 1218 HSP 10 Score: 79.337 bits (194), Expect = 1.897e-13 Identity = 50/166 (30.12%), Postives = 88/166 (53.01%), Query Frame = 1
Query: 1417 VLVGPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGS--FEQQKEGGKFKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGLVYGDRIEEIKPHKNFRLFLILDP--DCGGE--ISKAMRNRGLEICIIPENRELTLTDNDLLA 1896
+L G G GKT++V L+ +GN L ++++ D TDL G+ + +GG+F W D L+ AL+ G W++LD +N S SVL+ LN + G + + E G+ + + H+ ++F +P GG + ++ NR ++ + P LT+ D + +A
Sbjct: 1750 LLEGSPGVGKTSLVGALAKASGNTLVRINLSEQTDITDLFGADLPVEGGKGGEFAWRDGPLLAALKAGHWVVLDELNLASQSVLEGLNACFDHRGEIYVPELGMSFQVQ------HEKTKIFGCQNPFRQGGGRKGLPRSFLNRFTQVFVDP----LTVIDMEFIA 1905 HSP 11 Score: 77.0258 bits (188), Expect = 7.876e-13 Identity = 66/241 (27.39%), Postives = 112/241 (46.47%), Query Frame = 2
Query: 500 VSKKPLLLEGSPGVGKTSLVTQLAKLVGF----KVVRINLSEQTDANDLFGCFLPNENGKAGELSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPEINQKFMVEPGKTRLFACQNPVSEGSGRKGLP----KSFVNRF-TQVYMSSLSDSDYRNILNCLYPAIARETIGFIVEFVQ--NLREHVVVMKKFGTKGSP-----------------WEFNLRDLCRWCD 1138
S+ +LLEG G GKTSLV LA + G +++++ L +QTD+ L G + + GE W+ G L QA G WILL++++ A V+ L L++ GE+ IP V PG + FA + +S G G P + ++++ T++++ +L + +L YP++ + ++Q + H G + +P E +LRDL WC+
Sbjct: 320 ASQNAVLLEGPIGCGKTSLVEYLAAVTGRTKPPQLLKVQLGDQTDSKMLLGMYRCTD--VPGEFVWQPGTLTQAATMGHWILLEDIDYAPLDVVSVLIPLLEN-GELLIPGRGDCLKVAPG-FQFFATRRLLSCG-GNWYRPLNSHATLLDKYWTKIHLDNLDKRELNEVLQSRYPSLLAVVDHLLDIYIQLTGEKHHSWSDSSVGCEQAPEEVSEARRENKRPTLEGRELSLRDLLNWCN 555 HSP 12 Score: 75.0998 bits (183), Expect = 3.439e-12 Identity = 71/259 (27.41%), Postives = 114/259 (44.02%), Query Frame = 2
Query: 488 RALQVSKKPLLLEGSPGVGKTSLVTQLAKLVGFKVVRINLSEQTDANDLFGCFLP--------NENGKAGELSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPEINQ--------KFMVEPGKTRLFACQNPVSEGSGRKGLPKSFVNRFTQVYM-SSLSDSDYRNIL-NCLYPAIARETIG--------FIVEFVQNLREHVVVMKKFGTKGSPWEFNLRDLCRWCDLMVKHQHEPRKTRTPV 1186
RAL+ + P+LL G G GKT++ A L K+ ++ + +D G P E + W DGPL+QA+K + LLDE++LA SVLE LN+ L+ + + E + + K R+ A NP + G+K L + NRFT+++ S S D I+ + L P + I +++F+ L ++FG K ++RD+ W + M K E R +
Sbjct: 1378 RALEFGE-PVLLVGDTGCGKTTICQVFAALANQKLYSVSCHLHMETSDFLGGLRPVRQKPNDKEEIDTSRLFEWHDGPLVQAMKEDGFFLLDEISLADDSVLERLNSVLEVEKSLVLAEKGSPEDKDSEIELLTAGKKFRILATMNPGGD-FGKKELSPALRNRFTEIWCPQSTSREDLIQIISHNLRPGLCLGRIDPKGSDIPEVMLDFIDWL-----THQEFGRKCV---VSIRDILSWVNFMNKMGEEAALKRPEI 1626 HSP 13 Score: 72.4034 bits (176), Expect = 2.064e-11 Identity = 84/328 (25.61%), Postives = 150/328 (45.73%), Query Frame = 1
Query: 7063 KSYLVNMKHMECLSQQKLIIKGINELIEIVKNRFSPENKKINSSITESLLKFNDSNERAIIEIET-SLHVANDNFMDTDDSFD-GFVN----ELAVVIREKISAFKTI------LLTDNVDKS--------FIKVNT-HINALEQ--------SLDTQKIL----DLFTKIKSFFCDLNMNQVNKFRNLINEMV--LPKLRSLEKVEKEFLGLLLNYHRVLISATDALLTVTTKLMKEGFCIPNDSAWNNEAENSNPSEKMNDFEMAGMDGGVGTNDVTDEIESESQIE-----GNNKDKKDETENQPDIPDEDDGIEMTDDFNGKMH 7926
K + V + CLSQ + ++G+ L + E + ++ ESL +A+ + T H+ + + D GFV ++ + IR + A + + +N D++ F ++ + H+ L + +L QKI+ +L ++KS+ D + F + +V +P L S + FL + L HR L V T+L ++GFC+P + ++ E + + +D+E G+ G G DV+D+I +E Q+E G KDK+D +++ DI ED+ IEM++DF+GKMH
Sbjct: 4417 KDFEVCSSALSCLSQVSVHLQGLESLF--ILPGMEVEQRDSQMALVESLEYVRGEISKAMADFTTWKTHLLTSDSQGGNQMLDEGFVEDFSEQMEIAIRAILCAIQNLEERKNEKAEENTDQASPQEDYAGFERLQSGHLTKLLEDDFWADVSTLHVQKIISAISELLERLKSYGEDGTAAKHLFFSQSCSLLVRLVPVLSSYSDLVLFFLTMSLATHRSTAKLLSVLAQVFTELAQKGFCLPKEFMEDSAGEGAT---EFHDYEGGGIGEGEGMKDVSDQIGNEEQVEDTFQKGQEKDKED-PDSKSDIKGEDNAIEMSEDFDGKMH 4738 HSP 14 Score: 66.6254 bits (161), Expect = 1.130e-9 Identity = 45/158 (28.48%), Postives = 76/158 (48.10%), Query Frame = 1
Query: 1270 VLKIGQTQLSVNIANNTDNSKQ----FNKHLLLAGQLAILESMMLTVDMGWLTVLVGPAGTGKTTIVKLLSDITGN----HLKSMSMNSAMDTTDLLGSFEQQKEGGKFKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERG 1719
V+ GQ + N +S++ ++L+ L+++ + V +L GP G GKT++V+ L+ +TG L + + D+ LLG + G+F W TL QA G W++L+ +++ V+ L LLE NG LL+ RG
Sbjct: 273 VVLPGQLPAPGELGGNRSSSREQELALRSYVLVESVCKSLQTLAMAVASQNAVLLEGPIGCGKTSLVEYLAAVTGRTKPPQLLKVQLGDQTDSKMLLGMYRCTDVPGEFVWQPGTLTQAATMGHWILLEDIDYAPLDVVSVLIPLLE-NGELLIPGRG 429 HSP 15 Score: 64.3142 bits (155), Expect = 6.397e-9 Identity = 36/97 (37.11%), Postives = 60/97 (61.86%), Query Frame = 1
Query: 1570 FKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLE-PNGSLLLTERGLVYGDRIEEIKPHKNFRLFLILDP--DCGGE-ISKAMRNRGLEICI 1848
F +V+ TL QA+++G+W++LD +N + +L+ L+GLLE +GSL+L +RG E + H +FRLF ++P D G + +RNR E+ +
Sbjct: 816 FAFVEGTLAQAVKKGEWILLDEINLAAPEILECLSGLLEGSSGSLVLLDRG-----DTEPLVRHPDFRLFACMNPATDVGKRNLPPGIRNRFTELYV 907 HSP 16 Score: 55.4546 bits (132), Expect = 2.865e-6 Identity = 37/111 (33.33%), Postives = 56/111 (50.45%), Query Frame = 2
Query: 671 GELSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPEINQKFMVEPGKT-----RLFACQNPVSEGSGRKGLPKSFVNRFTQVYMSSLSDSDYRNILN 988
G W D L+QALK+GDW+L+D +N + SVL+ LNA L+ G + I E P T RLF +PV + ++ NR ++Y+S D+ + L+
Sbjct: 2220 GTFEWVDSMLVQALKSGDWLLMDNVNFCNPSVLDRLNALLEPGGVLTISERGMIDGSTPTITPNPNFRLFLSMDPV-----HGDISRAMRNRGLEIYISGEGDASTPDNLD 2325 HSP 17 Score: 53.5286 bits (127), Expect = 9.978e-6 Identity = 24/59 (40.68%), Postives = 39/59 (66.10%), Query Frame = 1
Query: 1372 ILESMMLTVDMGWLTVLVGPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGSFE 1548
++E + + V G +LVG GTGKT+ ++ L+ ITG+ L+ ++MN DT DLLG ++
Sbjct: 659 LIEQLAVCVSKGEPVLLVGETGTGKTSTIQYLAHITGHRLRVVNMNQQSDTADLLGGYK 717
BLAST of Midasin vs. Ensembl Human
Match: MDN1 (midasin AAA ATPase 1 [Source:HGNC Symbol;Acc:HGNC:18302]) HSP 1 Score: 329.331 bits (843), Expect = 1.183e-107 Identity = 182/376 (48.40%), Postives = 234/376 (62.23%), Query Frame = 2
Query: 89 VVTIRDLLSWVNFINISIGNKTSLYQ--------SIFHGAYMVIIDSINDKLNKS-FYEELLIKK--LDYLVKSSFPDIVECLDFQSSRF---------ELSVKENKVGIDPFYIELGSHSDWXXXXXXXXXXKNFQYSFNSKTTGSNVYRLFRALQVSKKPLLLEGSPGVGKTSLVTQLAKLVGFKVVRINLSEQTDANDLFGCFLPNENGKAGELSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPEINQKFMVEPGKTRLFACQNPVSEGSGRKGLPKSFVNRFTQVYMSSLSDSDYRNILNCLYPAIARETIGFIVEFVQNLREHVVVMKKFGTKGSPWEFNLRDLCRWCDLMVKHQ 1156
VV+IRD+LSWVNF+N +G + +L + S H A +V ID I + S F LL +K L +L+K IV ++Q + E + +N GI PF+I G + + + Y+ ++ TT N RL RA ++ KKP+LLEGSPGVGKTSLV LAK G +VRINLSEQTD DLFG LP E GK GE +WRDGPLL ALKAG W++LDE+NLASQSVLE LNAC DHRGEI++PE+ F V+ KT++F CQNP +G GRKGLP+SF+NRFTQV++ L+ D I + L+PAI + + +V F + V V KK+G KG PWEFNLRDL RWC LM+ Q
Sbjct: 1599 VVSIRDILSWVNFMN-KMGEEAALKRPEIISTVTSFVHAACLVYIDGIGSGVTSSGFGTALLARKECLKFLIKR-LAKIVRLTEYQKNELKIYDRMKAKEFTGIDNLWGIHPFFIPRGP---------VLHRNNIADYALSAGTTAMNAQRLLRATKL-KKPILLEGSPGVGKTSLVGALAKASGNTLVRINLSEQTDITDLFGADLPVEGGKGGEFAWRDGPLLAALKAGHWVVLDELNLASQSVLEGLNACFDHRGEIYVPELGMSFQVQHEKTKIFGCQNPFRQGGGRKGLPRSFLNRFTQVFVDPLTVIDMEFIASTLFPAIEKNIVKKMVAFNNQIDHEVTVEKKWGQKGGPWEFNLRDLFRWCQLMLVDQ 1962 HSP 2 Score: 85.1149 bits (209), Expect = 1.183e-107 Identity = 51/135 (37.78%), Postives = 77/135 (57.04%), Query Frame = 1
Query: 1159 RTKEDQDACGKVYNELADKSRFARFIPSGLDFYITDQVLKIG--QTQLSVNIANNTDNSKQFNKH--LLLAGQLAILESMMLTVDMGWLTVLVGPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGSFEQ 1551
RT+ED+ V+ ++ S + Y+ ++ +I QL ++ + ++H LLL LES+M V M W+ +LVGPA GKT++V+LL+ +TG+ LK M+MNSAMDTT+LLG FEQ
Sbjct: 1982 RTEEDKKKVIAVFKDV---------FGSNSNPYMGTRLFRITPYDVQLGYSVLSRGSCVPHPSRHPLLLLHQSFQPLESIMKCVQMSWMVILVGPASVGKTSLVQLLAHLTGHTLKIMAMNSAMDTTELLGGFEQ 2107 HSP 3 Score: 234.572 bits (597), Expect = 2.176e-60 Identity = 127/283 (44.88%), Postives = 183/283 (64.66%), Query Frame = 1
Query: 9145 WSQYESKTEYLSRQLCEMLRIVLEPTKANKLGGAYRTGKRLNMKKVIPYIASGFRKDKIWLRRNKPSQREYQVIIGVDNSKSMAYNVNHQLFFESLSIVLNGLTFLEVGQVGLCKFGAKAEVVLPLYEKFSSEIGDRLIETFDFSDKCSKFIPILEQSIKLFEENQNFLSSSAVRXXXXXXXXXXXXXXXXXXXXPKFISLIREAHNKGIFVVVILLDHADSKDSILNLKMVIDDG----CDFVRFLDVFPLQNYTVLQDVTKLPTLLTDVLRQWFELSSSAD 9981
W Y T LS++LCE LR++LEPT+A KL G YRTGKRLN++KVIPYIAS FRKDKIWLRR KPS+R+YQ+ + +D+S SM N QL FESL+++ N LT LEVGQ+ +C FG +++ P +E+FS G +++ F K +K LE +F Q LS + +T+QLLL++SDG + ++ ++ A N IFV+ ++LD+ S+DSIL++K+ I G + +++ FP Y +L+DV LP L+D LRQWFEL +++D
Sbjct: 5313 WQSYLILTAPLSQRLCEELRLILEPTQAAKLKGDYRTGKRLNIRKVIPYIASQFRKDKIWLRRTKPSKRQYQICLAIDDSSSMVDNHTKQLAFESLAVIGNALTLLEVGQIAVCSFGESVKLLHPFHEQFSDYSGSQILRLCKFQQKKTKIAQFLESVANMFAAAQQ-LSQNISSETAQLLLVVSDGRGLFLEGKERVLAAVQAARNANIFVIFVVLDNPSSRDSILDIKVPIFKGPGEMPEIRSYMEEFPFPYYIILRDVNALPETLSDALRQWFELVTASD 5594 HSP 4 Score: 135.961 bits (341), Expect = 1.231e-30 Identity = 82/177 (46.33%), Postives = 109/177 (61.58%), Query Frame = 1
Query: 1564 GKFKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGLVYGDRIEEIKPHKNFRLFLILDPDCGGEISKAMRNRGLEICIIPENRELTLTDNDLLAILITIYKLSVKNADSLLRFHKTMILAQSETPQIKIG----NINSLIQAAKLI----KDGYSLDQSL 2070
G F+WVDS LVQAL+ GDWL++D VNFC+ SVLDRLN LLEP G L ++ERG++ G I P+ NFRLFL +DP G+IS+AMRNRGLEI I E T + DL +L ++ + D LL H +ET +G ++++LIQ A LI + G SLD++
Sbjct: 2220 GTFEWVDSMLVQALKSGDWLLMDNVNFCNPSVLDRLNALLEPGGVLTISERGMIDGS-TPTITPNPNFRLFLSMDP-VHGDISRAMRNRGLEIYISGEGDASTPDNLDLKVLLHSLGLVGNSVCDILLALH-------TETRSTVVGSPTSSVSTLIQTAILIVQYLQRGLSLDRAF 2387 HSP 5 Score: 127.872 bits (320), Expect = 4.086e-28 Identity = 78/226 (34.51%), Postives = 115/226 (50.88%), Query Frame = 2
Query: 470 NVYRLFRALQVSKKPLLLEGSPGVGKTSLVTQLAKLVGFKVVRINLSEQTDANDLFGCFLPNENGKAGELSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPEINQKFMVEPGKTRLFACQNPVSEGSGRKGLPKSFVNRFTQVYMSSLSDSDYRNILN---CLYPAIARETIGFIVEFVQNLREHVVVMKKFGTKGSPWEFNLRDLCRWCD 1138
N+ + R + P+L++G VGKTSL+ LA G VRIN E TD + GC+ + +GK L +++G L+ A++ G WI+LDE+NLA VLE LN LD E+ + E + P + LFA QNP GRK L ++F NRF +++ L S+ IL+ L P+ + + +++ R V K G LRDL RW +
Sbjct: 1065 NLRDIVRVVSAGTYPVLIQGETSVGKTSLIQWLAAATGNHCVRINNHEHTDIQEYIGCYTSDSSGK---LVFKEGVLIDAMRKGYWIILDELNLAPTDVLEALNRLLDDNRELLVTETQEVVKAHP-RFMLFATQNPPGLYGGRKVLSRAFRNRFVELHFDELPSSELETILHKRCSLPPSYCSKLVKVMLDLQSYRRSSSVFAGKQGF------ITLRDLFRWAE 1280 HSP 6 Score: 118.627 bits (296), Expect = 2.500e-25 Identity = 85/347 (24.50%), Postives = 173/347 (49.86%), Query Frame = 1
Query: 5191 EEKQDTTVFRDCYRFAAALISS--DNQGIPSDDCKLFAANLSALNWEYETLSPGVPQNTIGRP---HDIYQDPIDVDNLLQAQKILPNANVSVTKLLGIYPEHTTXXXXXXXXXXXXXXSGDYSLIVFINNVERLIHEIQQWEVVAARHVSLIQHVDELNGLLVSWRHQELTMWENTISTANWQLERAVSREFFNHYNLI---VQESNKIQYDNDFESINLILEPILNSLLNSTVGNFEYRLKTMKLSIESLISNREIDYRFKDRLKNILLNHYDFYRLYLGNVQEHLSSEYKRIGKELRDFIKTKRWGKTP--PKITDVLKCHKIVFKYKRQWSGIINQPCTKFFV 6201
E K ++F CY+ A+L++ G+ +D +L + L A + TL P + + +P +D YQ P +V Q Q +L + +V+ LL +PEH L + ++ I + + F+N +E L+ + Q WE A+R +SL +H+D ++ +++ WR EL W ++ + ++ +F+ Y ++ +QE + Q D+ ++ L++ + + S++G F RL+ + + ++ +++ KD L ++L N Y +Y+ + VQ + + KEL++F+K +W V K H+ +FK+ +++ ++++PC V
Sbjct: 3648 EAKHYLSLFLSCYQTGASLVTHFYPLMGVELND-RLLGSQLLACTLSHNTLFGEAPSDLMVKPDGPYDFYQHP-NVPEARQCQPVLQGFSEAVSHLLQDWPEHPALEQLLVVMDRIRSFPLSSPISKFLNGLEILLAKAQDWEENASRALSLRKHLDLISQMIIRWRKLELNCWSMSLDNTMKRHTEKSTKHWFSIYQMLEKHMQEQTEEQEDDKQMTLMLLVSTLQAFIEGSSLGEFHVRLQMLLVFHCHVLLMPQVEG--KDSLCSVLWNLYHYYKQFFDRVQAKIVELRSPLEKELKEFVKISKWNDVSFWSIKQSVEKTHRTLFKFMKKFEAVLSEPCRSSLV 3990 HSP 7 Score: 88.1965 bits (217), Expect = 4.024e-16 Identity = 62/176 (35.23%), Postives = 93/176 (52.84%), Query Frame = 1
Query: 1393 TVDMGWLTVLVGPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGSFE--QQKEGGK--------FKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGLV--YGDRIEEIKPHKNFRLFLILDPDCGG-----EISKAMRNRGLEI-CIIPENRE 1866
++ G +LVG G GKTTI ++ + + L S+S + M+T+D LG +QK K F+W D LVQA+++ + +LD ++ SVL+RLN +LE SL+L E+G IE + K FR+ ++P GG E+S A+RNR EI C +RE
Sbjct: 1379 ALEFGEPVLLVGDTGCGKTTICQVFAALANQKLYSVSCHLHMETSDFLGGLRPVRQKPNDKEEIDTSRLFEWHDGPLVQAMKEDGFFLLDEISLADDSVLERLNSVLEVEKSLVLAEKGSPEDKDSEIELLTAGKKFRILATMNP--GGDFGKKELSPALRNRFTEIWCPQSTSRE 1552 HSP 8 Score: 85.1149 bits (209), Expect = 2.978e-15 Identity = 52/152 (34.21%), Postives = 82/152 (53.95%), Query Frame = 2
Query: 689 DGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGE--IFIPEINQKFMVEPGKTRLFACQNPVSEGSGRKGLPKSFVNRFTQVYMSSLSDSDYRNILNCLY---PAIARETIGFIVEFVQNLREHVVVMKKFGTKGSPWEFNLRDLCR 1129
+G L QA+K G+WILLDE+NLA+ +LECL+ L+ + + + + +V RLFAC NP ++ G++ LP NRFT++Y+ L + +L Y ++ + T+ I+ F LR+ GT P ++LR LCR
Sbjct: 820 EGTLAQAVKKGEWILLDEINLAAPEILECLSGLLEGSSGSLVLLDRGDTEPLVRHPDFRLFACMNPATD-VGKRNLPPGIRNRFTELYVEELESKEDLQVLIVDYLKGLSVNKNTVQGIINFYTALRKESGTKLVDGTGHRP-HYSLRTLCR 969 HSP 9 Score: 79.7221 bits (195), Expect = 1.280e-13 Identity = 53/161 (32.92%), Postives = 83/161 (51.55%), Query Frame = 1
Query: 1375 LESMMLTVDMGWLTVLV-GPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGSFEQQKEGGKFKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGLVYGDRIEEIKPHKNFRLFLILDPD--CGGE--ISKAMRNRGLEI 1842
L ++ V G VL+ G GKT++++ L+ TGNH ++ + D + +G + GK + + L+ A+R+G W+ILD +N VL+ LN LL+ N LL+TE E +K H F LF +P GG +S+A RNR +E+
Sbjct: 1066 LRDIVRVVSAGTYPVLIQGETSVGKTSLIQWLAAATGNHCVRINNHEHTDIQEYIGCYTSDS-SGKLVFKEGVLIDAMRKGYWIILDELNLAPTDVLEALNRLLDDNRELLVTETQ-------EVVKAHPRFMLFATQNPPGLYGGRKVLSRAFRNRFVEL 1218 HSP 10 Score: 79.337 bits (194), Expect = 1.897e-13 Identity = 50/166 (30.12%), Postives = 88/166 (53.01%), Query Frame = 1
Query: 1417 VLVGPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGS--FEQQKEGGKFKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGLVYGDRIEEIKPHKNFRLFLILDP--DCGGE--ISKAMRNRGLEICIIPENRELTLTDNDLLA 1896
+L G G GKT++V L+ +GN L ++++ D TDL G+ + +GG+F W D L+ AL+ G W++LD +N S SVL+ LN + G + + E G+ + + H+ ++F +P GG + ++ NR ++ + P LT+ D + +A
Sbjct: 1750 LLEGSPGVGKTSLVGALAKASGNTLVRINLSEQTDITDLFGADLPVEGGKGGEFAWRDGPLLAALKAGHWVVLDELNLASQSVLEGLNACFDHRGEIYVPELGMSFQVQ------HEKTKIFGCQNPFRQGGGRKGLPRSFLNRFTQVFVDP----LTVIDMEFIA 1905 HSP 11 Score: 77.0258 bits (188), Expect = 7.876e-13 Identity = 66/241 (27.39%), Postives = 112/241 (46.47%), Query Frame = 2
Query: 500 VSKKPLLLEGSPGVGKTSLVTQLAKLVGF----KVVRINLSEQTDANDLFGCFLPNENGKAGELSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPEINQKFMVEPGKTRLFACQNPVSEGSGRKGLP----KSFVNRF-TQVYMSSLSDSDYRNILNCLYPAIARETIGFIVEFVQ--NLREHVVVMKKFGTKGSP-----------------WEFNLRDLCRWCD 1138
S+ +LLEG G GKTSLV LA + G +++++ L +QTD+ L G + + GE W+ G L QA G WILL++++ A V+ L L++ GE+ IP V PG + FA + +S G G P + ++++ T++++ +L + +L YP++ + ++Q + H G + +P E +LRDL WC+
Sbjct: 320 ASQNAVLLEGPIGCGKTSLVEYLAAVTGRTKPPQLLKVQLGDQTDSKMLLGMYRCTD--VPGEFVWQPGTLTQAATMGHWILLEDIDYAPLDVVSVLIPLLEN-GELLIPGRGDCLKVAPG-FQFFATRRLLSCG-GNWYRPLNSHATLLDKYWTKIHLDNLDKRELNEVLQSRYPSLLAVVDHLLDIYIQLTGEKHHSWSDSSVGCEQAPEEVSEARRENKRPTLEGRELSLRDLLNWCN 555 HSP 12 Score: 75.0998 bits (183), Expect = 3.439e-12 Identity = 71/259 (27.41%), Postives = 114/259 (44.02%), Query Frame = 2
Query: 488 RALQVSKKPLLLEGSPGVGKTSLVTQLAKLVGFKVVRINLSEQTDANDLFGCFLP--------NENGKAGELSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPEINQ--------KFMVEPGKTRLFACQNPVSEGSGRKGLPKSFVNRFTQVYM-SSLSDSDYRNIL-NCLYPAIARETIG--------FIVEFVQNLREHVVVMKKFGTKGSPWEFNLRDLCRWCDLMVKHQHEPRKTRTPV 1186
RAL+ + P+LL G G GKT++ A L K+ ++ + +D G P E + W DGPL+QA+K + LLDE++LA SVLE LN+ L+ + + E + + K R+ A NP + G+K L + NRFT+++ S S D I+ + L P + I +++F+ L ++FG K ++RD+ W + M K E R +
Sbjct: 1378 RALEFGE-PVLLVGDTGCGKTTICQVFAALANQKLYSVSCHLHMETSDFLGGLRPVRQKPNDKEEIDTSRLFEWHDGPLVQAMKEDGFFLLDEISLADDSVLERLNSVLEVEKSLVLAEKGSPEDKDSEIELLTAGKKFRILATMNPGGD-FGKKELSPALRNRFTEIWCPQSTSREDLIQIISHNLRPGLCLGRIDPKGSDIPEVMLDFIDWL-----THQEFGRKCV---VSIRDILSWVNFMNKMGEEAALKRPEI 1626 HSP 13 Score: 72.4034 bits (176), Expect = 2.064e-11 Identity = 84/328 (25.61%), Postives = 150/328 (45.73%), Query Frame = 1
Query: 7063 KSYLVNMKHMECLSQQKLIIKGINELIEIVKNRFSPENKKINSSITESLLKFNDSNERAIIEIET-SLHVANDNFMDTDDSFD-GFVN----ELAVVIREKISAFKTI------LLTDNVDKS--------FIKVNT-HINALEQ--------SLDTQKIL----DLFTKIKSFFCDLNMNQVNKFRNLINEMV--LPKLRSLEKVEKEFLGLLLNYHRVLISATDALLTVTTKLMKEGFCIPNDSAWNNEAENSNPSEKMNDFEMAGMDGGVGTNDVTDEIESESQIE-----GNNKDKKDETENQPDIPDEDDGIEMTDDFNGKMH 7926
K + V + CLSQ + ++G+ L + E + ++ ESL +A+ + T H+ + + D GFV ++ + IR + A + + +N D++ F ++ + H+ L + +L QKI+ +L ++KS+ D + F + +V +P L S + FL + L HR L V T+L ++GFC+P + ++ E + + +D+E G+ G G DV+D+I +E Q+E G KDK+D +++ DI ED+ IEM++DF+GKMH
Sbjct: 4417 KDFEVCSSALSCLSQVSVHLQGLESLF--ILPGMEVEQRDSQMALVESLEYVRGEISKAMADFTTWKTHLLTSDSQGGNQMLDEGFVEDFSEQMEIAIRAILCAIQNLEERKNEKAEENTDQASPQEDYAGFERLQSGHLTKLLEDDFWADVSTLHVQKIISAISELLERLKSYGEDGTAAKHLFFSQSCSLLVRLVPVLSSYSDLVLFFLTMSLATHRSTAKLLSVLAQVFTELAQKGFCLPKEFMEDSAGEGAT---EFHDYEGGGIGEGEGMKDVSDQIGNEEQVEDTFQKGQEKDKED-PDSKSDIKGEDNAIEMSEDFDGKMH 4738 HSP 14 Score: 66.6254 bits (161), Expect = 1.130e-9 Identity = 45/158 (28.48%), Postives = 76/158 (48.10%), Query Frame = 1
Query: 1270 VLKIGQTQLSVNIANNTDNSKQ----FNKHLLLAGQLAILESMMLTVDMGWLTVLVGPAGTGKTTIVKLLSDITGN----HLKSMSMNSAMDTTDLLGSFEQQKEGGKFKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERG 1719
V+ GQ + N +S++ ++L+ L+++ + V +L GP G GKT++V+ L+ +TG L + + D+ LLG + G+F W TL QA G W++L+ +++ V+ L LLE NG LL+ RG
Sbjct: 273 VVLPGQLPAPGELGGNRSSSREQELALRSYVLVESVCKSLQTLAMAVASQNAVLLEGPIGCGKTSLVEYLAAVTGRTKPPQLLKVQLGDQTDSKMLLGMYRCTDVPGEFVWQPGTLTQAATMGHWILLEDIDYAPLDVVSVLIPLLE-NGELLIPGRG 429 HSP 15 Score: 64.3142 bits (155), Expect = 6.397e-9 Identity = 36/97 (37.11%), Postives = 60/97 (61.86%), Query Frame = 1
Query: 1570 FKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLE-PNGSLLLTERGLVYGDRIEEIKPHKNFRLFLILDP--DCGGE-ISKAMRNRGLEICI 1848
F +V+ TL QA+++G+W++LD +N + +L+ L+GLLE +GSL+L +RG E + H +FRLF ++P D G + +RNR E+ +
Sbjct: 816 FAFVEGTLAQAVKKGEWILLDEINLAAPEILECLSGLLEGSSGSLVLLDRG-----DTEPLVRHPDFRLFACMNPATDVGKRNLPPGIRNRFTELYV 907 HSP 16 Score: 55.4546 bits (132), Expect = 2.865e-6 Identity = 37/111 (33.33%), Postives = 56/111 (50.45%), Query Frame = 2
Query: 671 GELSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPEINQKFMVEPGKT-----RLFACQNPVSEGSGRKGLPKSFVNRFTQVYMSSLSDSDYRNILN 988
G W D L+QALK+GDW+L+D +N + SVL+ LNA L+ G + I E P T RLF +PV + ++ NR ++Y+S D+ + L+
Sbjct: 2220 GTFEWVDSMLVQALKSGDWLLMDNVNFCNPSVLDRLNALLEPGGVLTISERGMIDGSTPTITPNPNFRLFLSMDPV-----HGDISRAMRNRGLEIYISGEGDASTPDNLD 2325 HSP 17 Score: 53.5286 bits (127), Expect = 9.978e-6 Identity = 24/59 (40.68%), Postives = 39/59 (66.10%), Query Frame = 1
Query: 1372 ILESMMLTVDMGWLTVLVGPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGSFE 1548
++E + + V G +LVG GTGKT+ ++ L+ ITG+ L+ ++MN DT DLLG ++
Sbjct: 659 LIEQLAVCVSKGEPVLLVGETGTGKTSTIQYLAHITGHRLRVVNMNQQSDTADLLGGYK 717
BLAST of Midasin vs. Ensembl Human
Match: MDN1 (midasin AAA ATPase 1 [Source:HGNC Symbol;Acc:HGNC:18302]) HSP 1 Score: 120.553 bits (301), Expect = 3.290e-26 Identity = 68/179 (37.99%), Postives = 98/179 (54.75%), Query Frame = 2
Query: 470 NVYRLFRALQVSKKPLLLEGSPGVGKTSLVTQLAKLVGFKVVRINLSEQTDANDLFGCFLPNENGKAGELSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPEINQKFMVEPGKTRLFACQNPVSEGSGRKGLPKSFVNRFTQVYMSSLSDSDYRNILN--CLYP 1000
N+ + R + P+L++G VGKTSL+ LA G VRIN E TD + GC+ + +GK L +++G L+ A++ G WI+LDE+NLA VLE LN LD E+ + E + P + LFA QNP GRK L ++F NRF +++ L S+ IL+ C P
Sbjct: 992 NLRDIVRVVSAGTYPVLIQGETSVGKTSLIQWLAAATGNHCVRINNHEHTDIQEYIGCYTSDSSGK---LVFKEGVLIDAMRKGYWIILDELNLAPTDVLEALNRLLDDNRELLVTETQEVVKAHP-RFMLFATQNPPGLYGGRKVLSRAFRNRFVELHFDELPSSELETILHKRCSLP 1166 HSP 2 Score: 84.7297 bits (208), Expect = 2.826e-15 Identity = 52/152 (34.21%), Postives = 82/152 (53.95%), Query Frame = 2
Query: 689 DGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGE--IFIPEINQKFMVEPGKTRLFACQNPVSEGSGRKGLPKSFVNRFTQVYMSSLSDSDYRNILNCLY---PAIARETIGFIVEFVQNLREHVVVMKKFGTKGSPWEFNLRDLCR 1129
+G L QA+K G+WILLDE+NLA+ +LECL+ L+ + + + + +V RLFAC NP ++ G++ LP NRFT++Y+ L + +L Y ++ + T+ I+ F LR+ GT P ++LR LCR
Sbjct: 747 EGTLAQAVKKGEWILLDEINLAAPEILECLSGLLEGSSGSLVLLDRGDTEPLVRHPDFRLFACMNPATD-VGKRNLPPGIRNRFTELYVEELESKEDLQVLIVDYLKGLSVNKNTVQGIINFYTALRKESGTKLVDGTGHRP-HYSLRTLCR 896 HSP 3 Score: 80.1073 bits (196), Expect = 7.332e-14 Identity = 53/161 (32.92%), Postives = 83/161 (51.55%), Query Frame = 1
Query: 1375 LESMMLTVDMGWLTVLV-GPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGSFEQQKEGGKFKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGLVYGDRIEEIKPHKNFRLFLILDPD--CGGE--ISKAMRNRGLEI 1842
L ++ V G VL+ G GKT++++ L+ TGNH ++ + D + +G + GK + + L+ A+R+G W+ILD +N VL+ LN LL+ N LL+TE E +K H F LF +P GG +S+A RNR +E+
Sbjct: 993 LRDIVRVVSAGTYPVLIQGETSVGKTSLIQWLAAATGNHCVRINNHEHTDIQEYIGCYTSDS-SGKLVFKEGVLIDAMRKGYWIILDELNLAPTDVLEALNRLLDDNRELLVTETQ-------EVVKAHPRFMLFATQNPPGLYGGRKVLSRAFRNRFVEL 1145 HSP 4 Score: 64.6994 bits (156), Expect = 4.112e-9 Identity = 43/120 (35.83%), Postives = 64/120 (53.33%), Query Frame = 2
Query: 500 VSKKPLLLEGSPGVGKTSLVTQLAKLVGF----KVVRINLSEQTDANDLFGCFLPNENGKAGELSWRDGPLLQALKAGDWILLDEMNLAS-----QSVLECLNACLDHRGEIFIPEINQK 832
S+ +LLEG G GKTSLV LA + G +++++ L +QTD+ L G + + GE W+ G L QA G WILL++++ A QS L A +DH +I+I +K
Sbjct: 320 ASQNAVLLEGPIGCGKTSLVEYLAAVTGRTKPPQLLKVQLGDQTDSKMLLGMYRCTD--VPGEFVWQPGTLTQAATMGHWILLEDIDYAPLDVVLQSRYPSLLAVVDHLLDIYIQLTGEK 437 HSP 5 Score: 63.929 bits (154), Expect = 6.427e-9 Identity = 36/97 (37.11%), Postives = 60/97 (61.86%), Query Frame = 1
Query: 1570 FKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLE-PNGSLLLTERGLVYGDRIEEIKPHKNFRLFLILDP--DCGGE-ISKAMRNRGLEICI 1848
F +V+ TL QA+++G+W++LD +N + +L+ L+GLLE +GSL+L +RG E + H +FRLF ++P D G + +RNR E+ +
Sbjct: 743 FAFVEGTLAQAVKKGEWILLDEINLAAPEILECLSGLLEGSSGSLVLLDRG-----DTEPLVRHPDFRLFACMNPATDVGKRNLPPGIRNRFTELYV 834 HSP 6 Score: 55.8398 bits (133), Expect = 1.672e-6 Identity = 35/139 (25.18%), Postives = 65/139 (46.76%), Query Frame = 1
Query: 1270 VLKIGQTQLSVNIANNTDNSKQ----FNKHLLLAGQLAILESMMLTVDMGWLTVLVGPAGTGKTTIVKLLSDITGN----HLKSMSMNSAMDTTDLLGSFEQQKEGGKFKWVDSTLVQALRQGDWLILDTVNFCSASVL 1662
V+ GQ + N +S++ ++L+ L+++ + V +L GP G GKT++V+ L+ +TG L + + D+ LLG + G+F W TL QA G W++L+ +++ V+
Sbjct: 273 VVLPGQLPAPGELGGNRSSSREQELALRSYVLVESVCKSLQTLAMAVASQNAVLLEGPIGCGKTSLVEYLAAVTGRTKPPQLLKVQLGDQTDSKMLLGMYRCTDVPGEFVWQPGTLTQAATMGHWILLEDIDYAPLDVV 411
BLAST of Midasin vs. Ensembl Celegans
Match: F55F10.1 (Midasin [Source:UniProtKB/TrEMBL;Acc:O61851]) HSP 1 Score: 211.075 bits (536), Expect = 1.206e-65 Identity = 113/242 (46.69%), Postives = 153/242 (63.22%), Query Frame = 2
Query: 440 YSFNSKTTGSNVYRLFRALQVSKKPLLLEGSPGVGKTSLVTQLAKLVGFKVVRINLSEQTDANDLFGCFLP--NENGKAGELSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPEINQKFMVEPGKT--RLFACQNPVSEGSGRKGLPKSFVNRFTQVYMSSLSDSDYRNILNCLYPA--IARETIGFIVEFVQNLREHVVVMKKFGTKGSPWEFNLRDLCRWCDLMV 1147
+S + T N YR+ R L +SK P+LLEG+PG GK+S V LA+L G + R+NLS+QTD +DLFG +P E+G W DGP+L+A+K G+WILLDEMNLASQSVLE LNAC DHR ++I E+N++F + P + R FACQNP +G R+ LPKSF+NRFT +Y + L+ +D +LN + + E +V + L + + F G P FNLRDL RW DL+
Sbjct: 1353 FSLKAPTCAENFYRIARGLLISK-PILLEGAPGCGKSSTVMALAQLTGNPITRLNLSDQTDLSDLFGSDVPVLTEDGTMT-FRWEDGPVLKAIKKGEWILLDEMNLASQSVLEGLNACFDHRRVLYIAELNREFEI-PATSNCRFFACQNPRVQGGNRRALPKSFINRFTNIYTNDLTKNDIEIVLNGVDEKSLLRTEQRSAMVAISEQLEQ---ISGSFS--GGPHSFNLRDLLRWYDLLA 1586 HSP 2 Score: 61.6178 bits (148), Expect = 1.206e-65 Identity = 28/132 (21.21%), Postives = 74/132 (56.06%), Query Frame = 1
Query: 1159 RTKEDQDACGKVYNELADKSRFARFIPSGLDFYITDQVLKIGQTQLSVNIAN-NTDNSKQFNKHLLLAGQLAILESMMLTVDMGWLTVLVGPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGSFEQ 1551
R +ED++ ++ ++ ++ + + L+ + ++ ++IG+ L + ++ ++ L A Q ++ + V++ WL +LVGP GK ++++ ++++ G L+++++N+ D +L+GS+EQ
Sbjct: 1604 RREEDRELMRSIFEKMMEE----KLVTKALNLTVDEKEMRIGKCSLKRKTQQLGVQSPRKSLENRLFASQTILMHQIATCVELNWLALLVGPRNCGKRSVIENVANLCGKRLRTIALNADTDAQELIGSYEQ 1731 HSP 3 Score: 158.688 bits (400), Expect = 5.909e-38 Identity = 92/279 (32.97%), Postives = 155/279 (55.56%), Query Frame = 1
Query: 9145 WSQYESKTEYLSRQLCEMLRIVLEPTKANKLGGAYRTGKRLNMKKVIPYIASGFRKDKIWLRRNKPSQREYQVIIGVDNSKSMAYNVNHQLFFESLSIVLNGLTFLEVGQVGLCKFGAKAEVVLPLYEKFSSEIGDRLIETFDFSDKCSKFIPILEQSIKLFEENQNFLSSSAVRXXXXXXXXXXXXXXXXXXXXPKFISLIREAHN--KGIFVVVILLDHADSKDSILNLKMVIDDGCDFVRFLDVFPLQNYTVLQDVTKLPTLLTDVLRQWFELSSS 9975
W+ L+ +L E LR++LEP +ANK+ G YR+GKRLNM+++IPYIAS +RKD+IW+RR K +QREYQ++I VD+S+SM N HQ ES+ IV + L + G+V +C FGA ++P E +S +++ FS K + + +L+ + + +E + S + S LS G +R ++ +G+ V+ I+LD + D+ +L +FP Y +++ V +LP+++ + +RQWFE++++
Sbjct: 4093 WAAMSRTVGMLAAELAENLRLILEPQRANKMQGDYRSGKRLNMRRLIPYIASEYRKDRIWMRRTKRAQREYQILIAVDDSESMNENGIHQNTCESVCIVEDALRRCDAGRVSVCSFGADVNTIIPFGEASASS-SIEMLKQMTFSQKKTDLLLLLKTAKQQLDEMRTGTSEQMLIVISDGRGALSQGADK-----------VRALYSALQGVTVLFIVLDSGKKSIEDHTVASFKDNKVVLTPYLALFPFPFYALVKSVQQLPSVIAESIRQWFEMTTT 4359 HSP 4 Score: 141.354 bits (355), Expect = 1.067e-32 Identity = 99/332 (29.82%), Postives = 163/332 (49.10%), Query Frame = 2
Query: 182 AYMVIIDSINDKLNKSFYEELLIKKLDYLVKSSFPDIVECLDFQSS-RFELSV------KENKVGIDPFYIELGSHSDWXXXXXXXXXXKNFQYSFNSKTTGSNVYRLFRALQVSKKPLLLEGSPGVGKTSLVTQLAKLVGFKVVRINLSEQTDANDLFGCFLPNENGKAGELSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPEINQKFMVEPGKTRLFACQNPVSEGSGRKGLPKSFVNRFTQVYMSSLSDSDYRNILNCLYPAIARETIGFIVEFVQNLREHVVVMKKFGTKGSPWEFNLRDLCRWCDLMVKHQ 1156
A + D++ +++S YE + + L L S V + Q++ RF++ V EN V I +Y+E G+ + + +Y +KT N+ + R + + P+LLEG GKTS+V L+K G ++VRIN E TD + G ++ + G+ L +R+G L+QA++ G W++LDE+NLA V+E LN LD E+F+PEIN+ P + RLFA QNP +GRK L ++ ++RF + L + ++ C + ++E + LR + F + LRD+ RW M +
Sbjct: 643 ALLFTADNMFGSVDQSIYEAVSMAFLTNL--ESEEKKVMTVKIQAAFRFKVGVMPVPKHPENYVKIGGYYVEKGTQ----------LTESDPKYVI-TKTVKGNLSEIARIVCSGRFPILLEGETSAGKTSIVCHLSKATGNRIVRINNHEHTDVQEYMGSYVADAGGR---LVFREGALVQAVRDGSWVILDELNLAPTDVIEALNRLLDDNRELFVPEINETIKAHP-RFRLFATQNPAGSYAGRKRLSRALMSRFIVLRFHHLPIEELSEMV-CARCGVPPNASLKMIEVLNELRNRRSLSGLFSARDGL--MTLRDVFRWAKRMTTDE 954 HSP 5 Score: 98.9821 bits (245), Expect = 6.513e-20 Identity = 48/94 (51.06%), Postives = 63/94 (67.02%), Query Frame = 1
Query: 1567 KFKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGLVYGDRIEEIKPHKNFRLFLILDPDCGGEISKAMRNRGLEICI 1848
+F+W DS V A GDWL+++ VN CSA+VLDRLN LE G L++ ER Y E ++PH +FR+FL +D GEIS+AMRNR +EI I
Sbjct: 1801 RFEWTDSVFVDAYLHGDWLLIEDVNLCSAAVLDRLNSCLESGGKLVVAERQNSY----EPLEPHPDFRVFLSMDARV-GEISRAMRNRSVEIYI 1889 HSP 6 Score: 87.8113 bits (216), Expect = 1.995e-16 Identity = 57/164 (34.76%), Postives = 94/164 (57.32%), Query Frame = 1
Query: 1375 LESMMLTVDMGWL----TVLVGPAGTGKTTIVKLLSDITG-NHLKSMSMNSAMDTTDLLGSFEQQKEGGKFKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGLVYGDRIEEIKPHKNFRLFLILDP--DCG-GEISKAMRNRGLEI 1842
+ M++ + WL ++VG G GKTT LS++ G L+S++ + +T DLLG +++GG F+W D ++ A+R G L++D ++ SVL+RLN L E + +LLL++ G + E KP F++ ++P D G E+SKA+RNR E+
Sbjct: 1024 MRRMLVLTEQAWLRNEAVLMVGETGGGKTT----LSEVVGRGKLRSINCHERTETADLLGRLRPKQDGG-FEWSDGVVISAMRDGVPLLVDEISLAEDSVLERLNPLFEEDRALLLSDAGT--DTEVVESKP--GFQMIATMNPGGDYGKKELSKALRNRFTEV 1178 HSP 7 Score: 76.2554 bits (186), Expect = 5.662e-13 Identity = 45/125 (36.00%), Postives = 65/125 (52.00%), Query Frame = 1
Query: 1417 VLVGPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGSFEQQKEGGKFKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGLVYGDRIEEIKPHKNFRLFLILDP 1791
+L G GKT+IV LS TGN + ++ + D + +GS+ GG+ + + LVQA+R G W+ILD +N V++ LN LL+ N L + E E IK H FRLF +P
Sbjct: 749 LLEGETSAGKTSIVCHLSKATGNRIVRINNHEHTDVQEYMGSYVADA-GGRLVFREGALVQAVRDGSWVILDELNLAPTDVIEALNRLLDDNRELFVPEIN-------ETIKAHPRFRLFATQNP 865 HSP 8 Score: 73.9442 bits (180), Expect = 2.515e-12 Identity = 51/152 (33.55%), Postives = 82/152 (53.95%), Query Frame = 2
Query: 509 KPLLLEGSPGVGKTSLVTQLAKLVGFKVVR-INLSEQTDANDLFGCFLPNENGKAGELSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPEINQKFMVEPGKT--RLFACQNPVSEGSGRKGLPKSFVNRFTQVYMSS 955
+ +L+ G G GKT+L +++VG +R IN E+T+ DL G P ++G W DG ++ A++ G +L+DE++LA SVLE LN + + + + V K ++ A NP + G+K L K+ NRFT+V+ SS
Sbjct: 1039 EAVLMVGETGGGKTTL----SEVVGRGKLRSINCHERTETADLLGRLRPKQDGG---FEWSDGVVISAMRDGVPLLVDEISLAEDSVLERLNPLFEEDRALLLSDAGTDTEVVESKPGFQMIATMNPGGD-YGKKELSKALRNRFTEVWTSS 1182 HSP 9 Score: 72.0182 bits (175), Expect = 1.127e-11 Identity = 66/235 (28.09%), Postives = 111/235 (47.23%), Query Frame = 1
Query: 1417 VLVGPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGS-FEQQKEGGK--FKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGLVYGDRIEEIKPHKNFRLFLILDPDCGGEISKAMRNRGLEICIIPENRELTLTDNDLLAILITIYKLSVKNADSLLRF-HKTMILAQSETPQIKIG---------NINSLIQAAKLIKDGYSLDQSLRSVY 2082
+L G G GK++ V L+ +TGN + ++++ D +DL GS E G F+W D +++A+++G+W++LD +N S SVL+ LN + L + E +R EI N R F +P G +A+ + NR + NDL I I L+ + SLLR ++ ++A SE + G N+ L++ L+ G +L ++ VY
Sbjct: 1378 LLEGAPGCGKSSTVMALAQLTGNPITRLNLSDQTDLSDLFGSDVPVLTEDGTMTFRWEDGPVLKAIKKGEWILLDEMNLASQSVLEGLNACFDHRRVLYIAEL-----NREFEIPATSNCRFFACQNPRVQGGNRRALPKSFI-------NRFTNIYTNDLTKNDIEIV-LNGVDEKSLLRTEQRSAMVAISEQLEQISGSFSGGPHSFNLRDLLRWYDLLAAGRTLGEAFELVY 1599 HSP 10 Score: 67.3958 bits (163), Expect = 2.932e-10 Identity = 46/155 (29.68%), Postives = 80/155 (51.61%), Query Frame = 2
Query: 668 AGELSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPEINQKFMVEPGKTRLFACQNPVSEGSGRKGLPKSFVNRFTQVYMSSLSDSDYRNILNCLY-PAIARETIGFIVEFVQNLREHVVVMKKFGTKGSPWEFNLRDLCR 1129
A + + G +L+A + G W+L+DE+NLA ECL+A + + P + F RLFAC NP ++ +G++ LP RFT+ ++S +D +++ Y P +++ +FV+NL + + K+ P ++LR LCR
Sbjct: 513 AAPFALQKGAVLEAAEKGHWLLVDEINLAPP---ECLDAIVHA---LSAPGTHPNF-------RLFACMNPATD-AGKRRLPPGVRTRFTEFFVSETADPHQLSLIVSAYLPTVSQ-------QFVENLVKFYLSAKQL----YPSSYSLRTLCR 642
BLAST of Midasin vs. Ensembl Celegans
Match: F55F10.1 (Midasin [Source:UniProtKB/TrEMBL;Acc:O61851]) HSP 1 Score: 162.925 bits (411), Expect = 2.723e-39 Identity = 88/279 (31.54%), Postives = 153/279 (54.84%), Query Frame = 1
Query: 9145 WSQYESKTEYLSRQLCEMLRIVLEPTKANKLGGAYRTGKRLNMKKVIPYIASGFRKDKIWLRRNKPSQREYQVIIGVDNSKSMAYNVNHQLFFESLSIVLNGLTFLEVGQVGLCKFGAKAEVVLPLYEKFSSEIGDRLIETFDFSDKCSKFIPILEQSIKLFEENQNFLSSSAVRXXXXXXXXXXXXXXXXXXXXPKFISLIREAHN--KGIFVVVILLDHADSKDSILNLKMVIDDGCDFVRFLDVFPLQNYTVLQDVTKLPTLLTDVLRQWFELSSS 9975
W+ L+ +L E LR++LEP +ANK+ G YR+GKRLNM+++IPYIAS +RKD+IW+RR K +QREYQ++I VD+S+SM N HQ ES+ IV + L + G+V +C FGA ++P E +S +++ FS K + + +L+ + + +E + S + + + +R ++ +G+ V+ I+LD + D+ +L +FP Y +++ V +LP+++ + +RQWFE++++
Sbjct: 1862 WAAMSRTVGMLAAELAENLRLILEPQRANKMQGDYRSGKRLNMRRLIPYIASEYRKDRIWMRRTKRAQREYQILIAVDDSESMNENGIHQNTCESVCIVEDALRRCDAGRVSVCSFGADVNTIIPFGEASASS-SIEMLKQMTFSQKKTDLLLLLKTAKQQLDEMRTGTSEQML-----------IVISDGRGALSQGADKVRALYSALQGVTVLFIVLDSGKKSIEDHTVASFKDNKVVLTPYLALFPFPFYALVKSVQQLPSVIAESIRQWFEMTTT 2128
BLAST of Midasin vs. Ensembl Celegans
Match: vwa-8 (pep chromosome:WBcel235:X:12991447:12995827:1 gene:WBGene00008694.1 transcript:F11C1.5e.3 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:vwa-8) HSP 1 Score: 57.3806 bits (137), Expect = 1.967e-7 Identity = 37/106 (34.91%), Postives = 57/106 (53.77%), Query Frame = 1
Query: 1417 VLVGPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGSFEQQKEGGKFKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLE------PNGSLLLTER 1716
+L+GP G+GK+ I+ LS + +M ++ M+T +L+ KE G W DS LV A R GD +LD V +SV+ L+ L+ PNG+ L+ E+
Sbjct: 402 ILIGPKGSGKSRILGELSRRLNFNYVTMVLHQDMNTRELIQR-RHMKENGDTVWEDSILVTAARNGDVCVLDGVEMVHSSVIMSLSQLIYHRRFDLPNGNRLVGEK 506
BLAST of Midasin vs. Ensembl Celegans
Match: vwa-8 (pep chromosome:WBcel235:X:12991489:12995824:1 gene:WBGene00008694.1 transcript:F11C1.5e.2 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:vwa-8) HSP 1 Score: 57.3806 bits (137), Expect = 1.967e-7 Identity = 37/106 (34.91%), Postives = 57/106 (53.77%), Query Frame = 1
Query: 1417 VLVGPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGSFEQQKEGGKFKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLE------PNGSLLLTER 1716
+L+GP G+GK+ I+ LS + +M ++ M+T +L+ KE G W DS LV A R GD +LD V +SV+ L+ L+ PNG+ L+ E+
Sbjct: 402 ILIGPKGSGKSRILGELSRRLNFNYVTMVLHQDMNTRELIQR-RHMKENGDTVWEDSILVTAARNGDVCVLDGVEMVHSSVIMSLSQLIYHRRFDLPNGNRLVGEK 506
BLAST of Midasin vs. Ensembl Celegans
Match: vwa-8 (pep chromosome:WBcel235:X:12991476:12995827:1 gene:WBGene00008694.1 transcript:F11C1.5e.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:vwa-8) HSP 1 Score: 57.3806 bits (137), Expect = 1.967e-7 Identity = 37/106 (34.91%), Postives = 57/106 (53.77%), Query Frame = 1
Query: 1417 VLVGPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGSFEQQKEGGKFKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLE------PNGSLLLTER 1716
+L+GP G+GK+ I+ LS + +M ++ M+T +L+ KE G W DS LV A R GD +LD V +SV+ L+ L+ PNG+ L+ E+
Sbjct: 402 ILIGPKGSGKSRILGELSRRLNFNYVTMVLHQDMNTRELIQR-RHMKENGDTVWEDSILVTAARNGDVCVLDGVEMVHSSVIMSLSQLIYHRRFDLPNGNRLVGEK 506
BLAST of Midasin vs. Ensembl Fly
Match: CG13185 (gene:FBgn0033661 transcript:FBtr0310323) HSP 1 Score: 254.218 bits (648), Expect = 1.206e-66 Identity = 168/440 (38.18%), Postives = 243/440 (55.23%), Query Frame = 2
Query: 14 SNSVAGIIVRFLLIVQKMSHEINFSVVTIRDLLSWVNFINISIGNKTSLYQSIFHGAYMVIIDSINDKLNKSFYE-ELL--------IKKLDYLVKSSFPDIVECLDFQSSRFELSVKENKVGIDPFYIELGSHSDWXXXXXXXXXXKNFQYSFNSKTTGSNVYRLFRALQVSKKPLLLEGSPGVGKTSLVTQLAKLVGFKVVRINLSEQTDANDLFGCFLPNE------NGKAGELS-----WRDGPLLQALKAGD-WILLDEMNLASQSVLECLNACLDHRGEIFIPEINQKFMVEPGKTRLFACQNPVSEGSGRKGLPKSFVNRFTQVYMSSLSDSD-----------YRNILNCLYPAIARETI-----------------------GFIVEFVQNLREHVVVMKKFGTKGSPWEFNLRDLCRWCDLMVKHQHEPR 1168
++ VA +V +L ++ + + FSV RD+L+W N++ +S + Q+IF G + +D++ ++S + ELL IK+ +++ F + E + + + EL+ E + GI PF+I++ +S + F++ TT N++RL AL + +KP+LLEG PGVGKTS+V + +G+++VRINL E TD DLFG LP E N A E WRDGPLL ALKA + WILLDE+NLA QSVLE LNA LDHRGE++IPE+N+ F + G TR+FACQNP+ +G GRKGLP+SF+NRFT+VY+ LS D Y + LN + + + +V F + L V M +FG KG P+EFNLRD+ RWCDL+ H P+
Sbjct: 1638 THKVAEYLVDVVLHIRDTNDKFKFSV---RDILAWANYM-VSNAKLSFAEQAIF-GLETIFLDALEMLPHESQEQVELLKSNIVKQAIKQASLILQEKFT-LEELSEKRGTEVELN--ECRFGIRPFFIDVNQER---------LTSAKEDFLFDAPTTKQNLFRLLSALSL-QKPVLLEGPPGVGKTSIVESIGSAIGYQIVRINLCEHTDLADLFGTDLPAEDNLLESNTAATERQIRSFVWRDGPLLAALKAKNTWILLDELNLAPQSVLEGLNAVLDHRGEVYIPELNKSFHL-AGSTRIFACQNPLKQGGGRKGLPQSFLNRFTKVYLRKLSTEDLLHVVTEKYGKYFDQLNAYFLELLDQKAEGNLFEIYSGKESSCKSDSFDLGARLVRFSEQLDRGVASM-EFGYKGGPYEFNLRDILRWCDLL----HNPQ 2053 HSP 2 Score: 196.052 bits (497), Expect = 4.153e-49 Identity = 106/301 (35.22%), Postives = 167/301 (55.48%), Query Frame = 1
Query: 9142 TWSQYESKTEYLSRQLCEMLRIVLEPTKANKLGGAYRTGKRLNMKKVIPYIASGFRKDKIWLRRNKPSQREYQVIIGVDNSKSMAYNVNHQLFFESLSIVLNGLTFLEVGQVGLCKFGAKAEVVLPLYEKFSSEIGDRLIETFDFSDKCSKFIPIL--------------------EQSIKLFEENQNFLSSSAVRXXXXXXXXXXXXXXXXXXXXPKFISLIREAHNKGIFVVVILLDHADSKDSILNLKMV---IDDGCDFVRFLDVFPLQNYTVLQDVTKLPTLLTDVLRQWFELSSS 9975
TW ++ +R+LCE LR++LEPTK +L G YRTG+R+NMKK+IPYIAS FRKDKIWLRR KP+QR+Y++ I +D+SKSM +N + L E++S+V LT LE G++ + FG +++L E+F G RL+ +F+ +K +L E + + + +N S A + I+ A + IF+V I++D+ D+K+SIL+++ V D + +LD FP Y +++D+ +LP +L++ +RQWFEL +S
Sbjct: 5250 TWQGISNRMTQNARELCEQLRLILEPTKCTRLKGDYRTGRRINMKKIIPYIASQFRKDKIWLRRTKPAQRDYKITIAIDDSKSMHHNNSKTLTLEAISLVSQALTLLESGRLSIVSFGEAPQIILNHTEQFD---GPRLVNALNFAQDKTKIAGLLNFIRTANAEESGTGGDNGLFENLLLILSDGRNIFSEGAQNVKNA----------------------IKLARLQRIFLVYIIIDNPDNKNSILDIQHVAVNADGSVNINSYLDSFPFPYYVIVRDLNQLPLVLSEAMRQWFELVNS 5525 HSP 3 Score: 131.724 bits (330), Expect = 1.253e-29 Identity = 79/230 (34.35%), Postives = 122/230 (53.04%), Query Frame = 2
Query: 452 SKTTGSNVYRLFRALQVSKKPLLLEGSPGVGKTSLVTQLAKLVGFKVVRINLSEQTDANDLFGCFLPNENGKAGELSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPEINQKFMVEPGKTRLFACQNPVSEGSGRKGLPKSFVNRFTQVYMSSLSDSDYRNILN--C-LYPAIARETIGFIVEFVQNLREHVVVMKKFGTKGSPWEFNLRDLCRW 1132
+++ N+ L R + + K P+LL+G GKTSL+ +A+ G + +RIN E TD + G + + +GK L++R+G L+QA++ G WI+LDE+NLAS +LE LN LD E++I E P LFA QNP GRK L ++F NRF +++ + + + IL C + P AR+ + + + Q+ + S F LRDL RW
Sbjct: 1127 TESVKKNLKDLARIISIGKLPILLQGPTSAGKTSLIDYVARRSGNRCLRINNHEHTDLQEYIGTYAADLDGK---LTFREGVLVQAMRHGFWIILDELNLASTDILEALNRVLDDNRELYIAETQTLVKAHPN-FMLFATQNPPGLYGGRKTLSRAFKNRFIELHFADIPREELETILEKRCFIPPTYARKMVQCMTQLQQHRK-----------NTSKQVFTLRDLFRW 1341 HSP 4 Score: 110.923 bits (276), Expect = 2.422e-23 Identity = 67/178 (37.64%), Postives = 104/178 (58.43%), Query Frame = 1
Query: 1561 GGKFKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGLVYGDRIEEIKPHKNFRLFLILDPDCGGEISKAMRNRGLEICIIPENRELTLTDNDLLAILITIYKLSVKNADSLLRFHKTMILAQSETPQIKIGNINSLIQ----AAKLIKDGYSLDQSLRSVY 2082
GG F+WVDS +V +L+ G +++L+ VN CS++VLDRLN + EPNGSLL+ E+G+ D E + NFR FL +DP GE+S+AMRNR +E+ + + T + +DL A + ++ D +LR HK + S+ + +I+ L Q +A + GY L R++Y
Sbjct: 2335 GGSFEWVDSQIVTSLKCGQYILLEHVNLCSSAVLDRLNPVFEPNGSLLIAEKGISAQDSAEVVYKSTNFRAFLTVDPK-NGELSRAMRNRCVELSLSRD----TYSVDDLRAFVHEQGVREMEAIDCILRVHKGL----SQLTEFNNYSISHLTQFAFLSAAYRRIGYELK---RAIY 2500 HSP 5 Score: 88.1965 bits (217), Expect = 1.847e-16 Identity = 62/211 (29.38%), Postives = 110/211 (52.13%), Query Frame = 1
Query: 1363 QLAILESMMLTVDMGWLTVLVGPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGSFE--QQKEGGK-FKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGLV-----YGDRIEEIKPHKNFRLFLILDP--DCG-GEISKAMRNRGLEI-CIIPENRELTLTDNDLLAILITIYKLSVKNADSLLRFHK 1959
++A++ + L D L LVGP G GKTT+ +LL+ I L+ ++ + + D LG + KE + F+W D L+ A+++G + + D ++ SVL+RLN +LEP ++LL E+G + D + ++ F+ ++P D G E+S A+RNR E+ C+ +N+E DL+ I + ++ + + HK
Sbjct: 1440 RMAVVTAKALEFDEPVL--LVGPTGCGKTTVCQLLASIADVQLRILNCHMHTEGADFLGGLRPCRGKESTQLFEWADGPLIYAMQEGSYFMADEISLAEDSVLERLNCILEPERTVLLAEKGGISELDASPDFV--VQAKSGFQFLATMNPGGDFGKKELSPALRNRFTEVWCLPSDNKE------DLIEIAYNCMRRQKEHENQAINTHK 1640 HSP 6 Score: 82.0333 bits (201), Expect = 1.258e-14 Identity = 96/397 (24.18%), Postives = 170/397 (42.82%), Query Frame = 2
Query: 20 SVAGIIVRFLLIVQKMSHEINFSVVTIRDLLSWVNFI-----NISIGNKTSLYQSIFHGAYMVIIDSINDKLNKSFYEELLIKKLDYLVKSSFPDIVECLDFQSSRFELSVKENKVGIDPFYIELGSHSDWXXXXXXXXXXKNFQYSFNSKTTGSNVYRLF----RALQVSKKPLLLEGSPGVGKTSLVTQLAKLVGFKVVRINLSEQTDANDLFGCFLPNENGKAGEL-SWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPE--------INQKFMVEPGKT-RLFACQNPVSEGSGRKGLPKSFVNRFTQVY-MSSLSDSDYRNIL-NCLYPAIARETIGFIVEFVQNLREHVVVMKKFGTKGSPWEFNLRDLCRWCDLMV 1147
+ A +V+ + +Q+ + V T+RDL W N ++ N+ Q + Y+V+ + +S E LI+K + F + + LD Q+ F I+ S + + ++ +NF+ + T N+ R+ +AL+ + P+LL G G GKT++ LA + ++ +N T+ D G P ++ +L W DGPL+ A++ G + + DE++LA SVLE LN L+ + + E + F+V+ + A NP + G+K L + NRFT+V+ + S + D I NC+ E V VV+ ++F++RD+ W + MV
Sbjct: 1309 TYARKMVQCMTQLQQHRKNTSKQVFTLRDLFRWGNRYTHADKSLHDDNRYDWNQHLVEEGYLVLSSKV-----RSQEEHELIEK------TLFANFRKKLDLQTL---------------FDIQTDRESSSMVSRKILDAIRNFKLRDDIVWT-RNMTRMAVVTAKALEFDE-PVLLVGPTGCGKTTVCQLLASIADVQLRILNCHMHTEGADFLGGLRPCRGKESTQLFEWADGPLIYAMQEGSYFMADEISLAEDSVLERLNCILEPERTVLLAEKGGISELDASPDFVVQAKSGFQFLATMNPGGDF-GKKELSPALRNRFTEVWCLPSDNKEDLIEIAYNCMRRQKEHENQAINTHKVAEYLVDVVL--HIRDTNDKFKFSVRDILAWANYMV 1674 HSP 7 Score: 78.9518 bits (193), Expect = 1.187e-13 Identity = 48/161 (29.81%), Postives = 86/161 (53.42%), Query Frame = 1
Query: 1375 LESMMLTVDMGWLTVLV-GPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGSFEQQKEGGKFKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGLVYGDRIEEIKPHKNFRLFLILDPD--CGGE--ISKAMRNRGLEI 1842
L+ + + +G L +L+ GP GKT+++ ++ +GN ++ + D + +G++ +G K + + LVQA+R G W+ILD +N S +L+ LN +L+ N L + E + +K H NF LF +P GG +S+A +NR +E+
Sbjct: 1134 LKDLARIISIGKLPILLQGPTSAGKTSLIDYVARRSGNRCLRINNHEHTDLQEYIGTYAADLDG-KLTFREGVLVQAMRHGFWIILDELNLASTDILEALNRVLDDNRELYIAETQTL-------VKAHPNFMLFATQNPPGLYGGRKTLSRAFKNRFIEL 1286 HSP 8 Score: 78.1814 bits (191), Expect = 2.046e-13 Identity = 52/150 (34.67%), Postives = 81/150 (54.00%), Query Frame = 2
Query: 692 GPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPEINQKFMVEPGKT-RLFACQNPVSEGSGRKGLPKSFVNRFTQVYMSSLSDSDYRNILNCLYPA---IARETIGFIVEFVQNLREHVVVMKKFGTKGSPWEFNLRDLCR 1129
G L+ + GDW+LLDE+NLAS LECL+ L+ G + + E V+ R+FAC NP ++ G+K LP NRFT+ ++ L+ ++L Y A I R+++ IV+ ++LR+ + G P ++LR LCR
Sbjct: 892 GSLVNCISNGDWVLLDEINLASAETLECLSTILEPEGSVVLLERGDFTPVKRHPDFRIFACMNPNTD-IGKKDLPVGIRNRFTEFFVDELTTDADLSLLVSDYLANTGIQRKSVHNIVQLYKSLRKLSELQLNDGLGNRPV-YSLRTLCR 1039 HSP 9 Score: 75.485 bits (184), Expect = 1.280e-12 Identity = 46/133 (34.59%), Postives = 71/133 (53.38%), Query Frame = 1
Query: 7543 VLPKLRSLEKVEKEFLGLLLNYHRVLISATDALLTVTTKLMKEGFCIPNDSAWNNEAENSNPSEKMNDFEMAGMDGGVGTNDVTDEIESESQIEGNNK--DKKDETENQPDIPDEDDGIEMTDDFNGKMHDPE 7935
+LP L + + L L H+V +LTV ++ +GFC+P D + E ++ S+ F G++ G G ND +D+IESE Q++ + D+KDE + Q E+ GIEM+DDF+ KM D E
Sbjct: 4546 LLPVLEQYQLLADYLLLQQLATHKVSAKMLSIVLTVFVEIGSKGFCVPPDLMQDEEGQSKQESKNGEGF---GLEDGTGENDASDKIESEDQLDDAKRPEDRKDEGDKQEPDCKEEKGIEMSDDFDAKMQDVE 4675 HSP 10 Score: 67.3958 bits (163), Expect = 3.220e-10 Identity = 45/139 (32.37%), Postives = 79/139 (56.83%), Query Frame = 1
Query: 1570 FKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGLVYGDRIEEIKPHKNFRLFLILDP--DCG-GEISKAMRNRGLEICIIPENRELTLTDNDLLAILITIY----KLSVKNADSLLRFHKTM 1965
F ++ +LV + GDW++LD +N SA L+ L+ +LEP GS++L ERG +K H +FR+F ++P D G ++ +RNR E + ELT TD D L++L++ Y + K+ ++++ +K++
Sbjct: 887 FAFIPGSLVNCISNGDWVLLDEINLASAETLECLSTILEPEGSVVLLERG-----DFTPVKRHPDFRIFACMNPNTDIGKKDLPVGIRNRFTEFFV----DELT-TDAD-LSLLVSDYLANTGIQRKSVHNIVQLYKSL 1014 HSP 11 Score: 54.6842 bits (130), Expect = 2.157e-6 Identity = 32/113 (28.32%), Postives = 55/113 (48.67%), Query Frame = 1
Query: 1417 VLVGPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLG-------------SFEQQKEGGKFKWVDSTLVQALR-QGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTE 1713
+L GP G GKT+IV+ + G + +++ D DL G + +++ F W D L+ AL+ + W++LD +N SVL+ LN +L+ G + + E
Sbjct: 1797 LLEGPPGVGKTSIVESIGSAIGYQIVRINLCEHTDLADLFGTDLPAEDNLLESNTAATERQIRSFVWRDGPLLAALKAKNTWILLDELNLAPQSVLEGLNAVLDHRGEVYIPE 1909
BLAST of Midasin vs. Ensembl Zebrafish
Match: mdn1 (midasin AAA ATPase 1 [Source:ZFIN;Acc:ZDB-GENE-041001-138]) HSP 1 Score: 329.331 bits (843), Expect = 2.775e-89 Identity = 180/387 (46.51%), Postives = 237/387 (61.24%), Query Frame = 2
Query: 89 VVTIRDLLSWVNFIN----------ISIGN----------KTSLYQSIFHGAYMVIIDSINDKLNKSFYEELLIKK---LDYLVK--SSFPDIVECLDFQSSRFELSVK------ENKVGIDPFYIELGSHSDWXXXXXXXXXXKNFQYSFNSKTTGSNVYRLFRALQVSKKPLLLEGSPGVGKTSLVTQLAKLVGFKVVRINLSEQTDANDLFGCFLPNENGKAGELSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPEINQKFMVEPGKTRLFACQNPVSEGSGRKGLPKSFVNRFTQVYMSSLSDSDYRNILNCLYPAIARETIGFIVEFVQNLREHVVVMKKFGTKGSPWEFNLRDLCRWCDLMVKHQ 1156
++++RD+LSWVNF+N +++G K + H A +V +D I S E L+ + L+YL K S ++ E + + SV E GIDPFYI GS S+ LS Y+ ++ TT N R+ RAL++ ++P+LLEGSPGVGKTSLV LAK G +VRINLSEQTD DLFG LP E GK GE +WRDGPLL LKAG WI+LDE+NLASQSVLE LNAC DHR EI+IPE+ +F V+ KT++F CQNP ++G GRKGLPKSF+NRFTQVY+ +L++ D + I + ++P I I +V+F L + V+V +K+G +G PWEFNLRDL RWC LM Q
Sbjct: 1572 ILSVRDILSWVNFMNTVCERDEDGFMTMGELEEEEPDWDLKLDTVTAFIHAACLVYVDGIGSGTTVSAAENALVARKLSLEYLEKRLSRITELDEEMQEAIRVYNTSVSREPQWGEGFFGIDPFYIAAGSESEGRSLS---------DYALSAGTTAVNAQRILRALKL-QRPILLEGSPGVGKTSLVAALAKASGNHLVRINLSEQTDVTDLFGTDLPVEGGKGGEFAWRDGPLLAGLKAGHWIVLDELNLASQSVLEGLNACFDHRAEIYIPELGMRFHVQQEKTKIFGCQNPFTQGGGRKGLPKSFLNRFTQVYVDALTNEDMQFIGDSIFPNIDNAIISKMVQFSNRLVQEVMVDRKWGQRGGPWEFNLRDLFRWCQLMQTDQ 1948 HSP 2 Score: 234.958 bits (598), Expect = 1.091e-60 Identity = 123/284 (43.31%), Postives = 187/284 (65.85%), Query Frame = 1
Query: 9145 WSQYESKTEYLSRQLCEMLRIVLEPTKANKLGGAYRTGKRLNMKKVIPYIASGFRKDKIWLRRNKPSQREYQVIIGVDNSKSMAYNVNHQLFFESLSIVLNGLTFLEVGQVGLCKFGAKAEVVLPLYEKFSSEIGDRLIETFDFSDKCSKFIPILEQSIKLFEENQNFLSSSAVRXXXXXXXXXXXXXXXXXXXXPKFISLIREAHNKGIFVVVILLDHADSKDSILNLKMVIDDG----CDFVRFLDVFPLQNYTVLQDVTKLPTLLTDVLRQWFELSSSADK 9984
W QY++ T LS+QLCE LR+VLEPT+A KL G +RTGKRLNM+KVIPYIAS FRKDKIWLRR KPS+R YQ+ + VD+S SM N QL FE++S+++N LT LEVGQV +C FG +++ P +++F+ + G +++ F K ++ LE S +F + + + +TSQLLL++SDG + + ++ A + +FV+ ++LD+ +S+DSIL++K+ I G + +++ FP Y +L++V LP L+D LRQWFEL ++ ++
Sbjct: 5240 WHQYQTLTSPLSQQLCEQLRLVLEPTQAAKLRGDFRTGKRLNMRKVIPYIASQFRKDKIWLRRTKPSKRNYQICLAVDDSSSMVDNHTKQLAFEAVSVIVNALTLLEVGQVSVCSFGETVQLLHPFHQQFNDQTGAKILRLCQFQQKKTRIAQFLETSTNMFMAAKQQIPGATNTETSQLLLIVSDGRGLFLEGKERVATAVQAARSANVFVIFVVLDNPNSRDSILDIKVPIFKGPGELPEIRSYMEEFPFPFYVILRNVNGLPETLSDALRQWFELVTATEQ 5523 HSP 3 Score: 140.198 bits (352), Expect = 4.842e-32 Identity = 86/198 (43.43%), Postives = 115/198 (58.08%), Query Frame = 1
Query: 1516 MDTTDLLGSFEQQKEG---GKFKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGLVYGDRIEEIKPHKNFRLFLILDPDCGGEISKAMRNRGLEICIIPENRELTLTDNDLLAILITIYKLSVKNADSLLRFHKTMILAQSETPQIKIGNINSLIQAAKL----IKDGYSLDQSLRSVYGD 2088
MD T L Q ++G G F+W+D TLVQAL+ GDWL++D VNFCS SVLDRLN LLEP GSL + ERG++ G I PH NFRLFL +DP GEIS+AMRNRG+EI I E+ + DL +L T+ D L+ H + ++P +++SL+ AA L ++ G L +L G+
Sbjct: 2188 MDFTLLKERLAQMQDGWTHGGFEWLDGTLVQALQAGDWLLMDNVNFCSPSVLDRLNALLEPGGSLTINERGIIDGT-TPTITPHPNFRLFLTMDP-THGEISRAMRNRGVEIYIPGEHEGVCWDTLDLKMVLHTLGVTGDCVCDLLISIHSEIKKTIWDSP---ASSMSSLLHAASLLSGQLQRGVDLPSALLQACGE 2380 HSP 4 Score: 137.887 bits (346), Expect = 2.281e-31 Identity = 120/427 (28.10%), Postives = 183/427 (42.86%), Query Frame = 2
Query: 2 EIEKSNSVAGIIVRFL-LIVQKMSHEINFSVVTIRDLLSWVNFINISIGNKTSLYQSIFHGAYMVIIDSINDK----------------------LNKSFYEELLIKKLDYLVKSSFPDI------------VECLD-----------FQSSRFELSVKENKVGIDPFYIELGSHSDWXXXXXXXXXXKNFQYSFNSKTTGSNVYRLFRALQVSKKPLLLEGSPGVGKTSLVTQLAKLVGFKVVRINLSEQTDANDLFGCFLPNENGKAGELSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPEINQKFMVEPGKTRLFACQNPVSEGSGRKGLPKSFVNRFTQVYMSSLSDSDYRNILN--CLYPAIARETIGFIVEFVQNLREHVVVMKKFGTKGSPWEFNLRDLCRWCD 1138
++ K N GI RF L V+++ E + ++ + D L +N I S Y ++ A ++D K + +S YE + L L +SS P + V+CL Q + LS + + +DP YI + + N+ L R + P+LL+G VGKTSL+ LA G + VRIN E TD + GC+ +E+GK L +++G L+ A++ G WI+LDE+NLA VLE LN LD E+F+ E + P + LFA QNP GRK L ++F NRF +++ + L S+ NIL+ C P + +++ +Q+LR V G LRDL RW +
Sbjct: 865 DVGKRNLPLGIRNRFTELYVEELESESDLRIL-VSDYLKGLNLSRGIIHGIISFYLAVRKEANTRLVDGTGHKPHYSLRTLCRALKFVASDPCYNIQRSLYEGFCLSFLTQLDRSSHPIVQKLVCQYILGGNVKCLRQPIPQPKDRVCVQIDEYWLSQGDKEPAVDPSYI-------------------------LTPSVKLNLKDLARVVAAGIHPVLLQGETSVGKTSLIKWLAASTGNQCVRINNHEHTDIQEYIGCYSSDEHGK---LVFKEGVLIDAMRKGYWIILDELNLAPTDVLEALNRLLDDNRELFVAETQEVVKAHP-RFMLFATQNPPGLYGGRKVLSRAFRNRFVELHFNELPSSELENILHQRCSLPPSYCSKLIKVMQDLQSLRRGSSVF-----AGKHGYITLRDLFRWAE 1256 HSP 5 Score: 90.1225 bits (222), Expect = 5.431e-17 Identity = 69/196 (35.20%), Postives = 102/196 (52.04%), Query Frame = 1
Query: 1363 QLAILESMMLTVDMGWLTVLVGPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGSFE-------QQ---KEGGKFKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGLVYGD-RIEEIKPHKNFRLFLILDPDCGG-----EISKAMRNRGLEICIIPENRELTLTDNDLLAIL 1902
+LA+L L G +LVG G GKTTI +L + + G S++ + M+T+D LG QQ ++G F+W D L+ A++Q ++D ++ SVL+RLN +LEP SL+L E+G GD +E I FRL ++P GG E+S A+RNR EI N + NDL+ I+
Sbjct: 1347 RLAVLVGRALR--FGESVLLVGDTGCGKTTICQLFAALAGQKFFSLNCHLHMETSDFLGGLRPVRHTGTQQADAEDGRLFEWHDGPLILAMKQDGVFLMDEISLADDSVLERLNSVLEPEKSLVLAEKG--SGDEEVELIIAGNKFRLVATMNP--GGDFGKKELSPALRNRFTEIWCPQSN-----SRNDLIQII 1531 HSP 6 Score: 87.8113 bits (216), Expect = 3.400e-16 Identity = 101/437 (23.11%), Postives = 193/437 (44.16%), Query Frame = 1
Query: 5371 PHDIYQDPIDVDNLLQAQKILP---NANVSVTKLLGIYPEHTTXXXXXXXXXXXXXXSGDYSLIVFINNVERLIHEIQQWEVVAARHVSLIQHVDELNGLLVSWRHQELTMWENTISTANWQLERAVSREFFNHYNLI----VQESNKIQYDNDFESINLILEPILNSLL--NSTVGNFEYRLKT-MKLSIESLISNREIDYRFKDRLKNILLNHYDFYRLYLGNVQEHLSSEYKRIGKELRDFIKTKRWGKTP--PKITDVLKCHKIVFKYKRQWSGIINQPCTKFFV----------------LTPI----NALKQVNLIDIGNCIESD----------------IDFSKSKLSDWCATMYEYSR--EMWETMENFQKE----TKELEDISVVQSKSEDDKEALEKKWRMNVKNLQQRKRNCVSQFVKELREMGL 6519
P+D Y++P N+ QA+ LP + +V + L +PEH L+ I I IL S L F+N +E L+ + Q WE A+R VSL ++ + L++ WR EL W +++ A + ++ + + Y LI ++ ++D+ E+++L ST+G F RL + L++ ++ + D L ++L N Y +Y + ++Q ++ + KEL+DF+K +W V K H+ +FK+ +++ + +PC V TP+ ALK+ L G +++D + K+ C + + + E+ E ++ F E + L+ ++V D+ ++K + VK++ Q+K+ ++ K L ++GL
Sbjct: 3688 PYDFYREP----NMSQARLCLPALEQLSKAVRQRLNDWPEHPALVQIIMVIERILAFSLSSPLAKFLNGLEILLSKAQDWENNASRAVSLRNELEVVTQLIIQWRKLELNCWSSSLDNALKRHAENSTKHWLSIYQLIEKYLQEQKTNSRHDDGVENLSLSSVSSSLQAFIEGSTLGEFSVRLSMLLGFHCHILLAPKQEGH---DSLCSLLWNLYKYYSQFSPSIQTKITHLRSPLEKELKDFVKISKWNDVSFWSIKHSVEKTHRTLFKFVKKFEAALKEPCAPCLVEQGSSSSMDDVDSQPEETPLLRVHRALKEA-LPGKGRDLQADGPEELSSENPSSLQARLPVLTRKMKKLCVQLVKKTSLPELSEDLDQFTAEIILNVQALQGLTV-------DRSMEQEKQKAEVKHILQQKQRALADLFKMLAQIGL 4109 HSP 7 Score: 82.8037 bits (203), Expect = 1.011e-14 Identity = 56/156 (35.90%), Postives = 89/156 (57.05%), Query Frame = 1
Query: 1252 FYITDQVLKIGQT--QLSVNIANNTDNSKQFNKHLLLAGQLAILESMMLTVDMGWLTVLVGPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGSFEQQKEGGKFKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTE 1713
F+IT L++G + Q S D H L LE++M V+MGW+ +LVGP +GK+++V LL+ +TG+ L+ M+MNSAMDTT+LLG FEQ VD +++ +Q +L+++++ A V R GL+ +G++ TE
Sbjct: 1997 FHITPLNLQLGYSVLQRSGGAPIALDPPLSITHH-----SLRPLEALMKCVEMGWMVILVGPTASGKSSLVHLLALLTGHRLRVMAMNSAMDTTELLGGFEQ---------VD--IIRPWQQ----VLESMDYVVAMVTRR--GLMSLDGAVQDTE 2130 HSP 8 Score: 82.4185 bits (202), Expect = 1.389e-14 Identity = 53/152 (34.87%), Postives = 83/152 (54.61%), Query Frame = 2
Query: 689 DGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGE--IFIPEINQKFMVEPGKTRLFACQNPVSEGSGRKGLPKSFVNRFTQVYMSSL-SDSDYRNILNCLYPA--IARETIGFIVEFVQNLREHVVVMKKFGTKGSPWEFNLRDLCR 1129
+G L QA+K G+WILLDE+NLA+ LECL+ L+ + + + + + RLFAC NP ++ G++ LP NRFT++Y+ L S+SD R +++ ++R I I+ F +R+ GT P ++LR LCR
Sbjct: 797 EGTLAQAVKKGEWILLDEINLAAAETLECLSGLLEGSSGSLVLLDRGDTEPLFRHSDFRLFACMNPATD-VGKRNLPLGIRNRFTELYVEELESESDLRILVSDYLKGLNLSRGIIHGIISFYLAVRKEANTRLVDGTGHKP-HYSLRTLCR 946 HSP 9 Score: 80.1073 bits (196), Expect = 6.821e-14 Identity = 83/309 (26.86%), Postives = 165/309 (53.40%), Query Frame = 1
Query: 7453 ILDLFTKIKSFFCDLNMNQVNKFRNLINEMVL--PKLRSLEKVEKEFLGLLLNYHRVLISATDALLTVTTKLMKEGFCIPNDSAWNNEAENSNPSEKMNDFEMAGMDGGVGTNDVTDEIESESQIEGN---NKDKKDETENQPDIPDEDDGIEMTDDFNGKMHD-PEIXXXXXXXXXXXXXXXXXXXMGEVGNAPETDVNXXXXXXXXXXXXXXXXXXXXXXXXGGVSEQNTTEMVADEKEDKDHXXXXXXXXXXXXXXXXXXXXXXTDAGNEEDIGEFKDDEDSKDQENAPENTGQEPEKLDNTEEEN 8361
+ +L +++S D + + + R +V P L ++ + +L +L+ HR L V T+L ++G+C+P + + E + + +D+E G+ G G DV+D+IE+E Q+E KDK +E +++ DI +ED+ IEM++DF+GKMHD + + ++ +++++E +L+ KMG++G+ +TD E + ++E+ +D ++++ G +Q +E+VA KD N+ + +NK+ +++ + E E + E + DE+ D + + EPE ++ EE N
Sbjct: 4524 VTELLERLRSHRDDCQPHHLQELREACRSVVRLEPMLCLYSELVRYYLAVLMGAHRTTGKLLSVLANVFTELAQKGYCLPQELMGGGDGEGAT---EFHDYEGGGIGEGEGVKDVSDKIENEDQVEDTFQEGKDKDEEEQDKQDIKEEDNAIEMSEDFDGKMHDGTDREPGEDEEDSDKEDEELDKKMGDLGDG-QTDTLDERMWGDDDDDEDNNDDSDKEEESGPGMDQGESELVA-----KDDNTDAGESNKDKNQDKDLPKDEEEKEKIHEQVDEREFDENEVDPYHGKQEKKAEPEAMELPEELN 4823 HSP 10 Score: 80.1073 bits (196), Expect = 6.878e-14 Identity = 44/151 (29.14%), Postives = 80/151 (52.98%), Query Frame = 1
Query: 1309 ANNTDNSKQFNKHLLLAGQLAI-LESMMLTVDMGWLTVLVGPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGS--FEQQKEGGKFKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGLVYGDRIEEIK 1752
A + + + + L AG A+ + ++ + + +L G G GKT++V L+ +GNHL ++++ D TDL G+ + +GG+F W D L+ L+ G W++LD +N S SVL+ LN + + + E G+ + + E+ K
Sbjct: 1699 AGSESEGRSLSDYALSAGTTAVNAQRILRALKLQRPILLEGSPGVGKTSLVAALAKASGNHLVRINLSEQTDVTDLFGTDLPVEGGKGGEFAWRDGPLLAGLKAGHWIVLDELNLASQSVLEGLNACFDHRAEIYIPELGMRFHVQQEKTK 1849 HSP 11 Score: 74.3294 bits (181), Expect = 3.583e-12 Identity = 48/147 (32.65%), Postives = 75/147 (51.02%), Query Frame = 1
Query: 1414 TVLVGPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGSFEQQKEGGKFKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGLVYGDRIEEIKPHKNFRLFLILDPD--CGGE--ISKAMRNRGLEI 1842
+L G GKT+++K L+ TGN ++ + D + +G + E GK + + L+ A+R+G W+ILD +N VL+ LN LL+ N L + E E +K H F LF +P GG +S+A RNR +E+
Sbjct: 1056 VLLQGETSVGKTSLIKWLAASTGNQCVRINNHEHTDIQEYIGCY-SSDEHGKLVFKEGVLIDAMRKGYWIILDELNLAPTDVLEALNRLLDDNRELFVAETQ-------EVVKAHPRFMLFATQNPPGLYGGRKVLSRAFRNRFVEL 1194 HSP 12 Score: 73.559 bits (179), Expect = 5.400e-12 Identity = 53/166 (31.93%), Postives = 81/166 (48.80%), Query Frame = 2
Query: 488 RALQVSKKPLLLEGSPGVGKTSLVTQLAKLVGFKVVRINLSEQTDANDLFGCFLP-------NENGKAGEL-SWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPEINQ-----KFMVEPGKTRLFACQNPVSEGSGRKGLPKSFVNRFTQVY 946
RAL+ + LL+ G G GKT++ A L G K +N + +D G P + + G L W DGPL+ A+K L+DE++LA SVLE LN+ L+ + + E + ++ K RL A NP + G+K L + NRFT+++
Sbjct: 1354 RALRFGESVLLV-GDTGCGKTTICQLFAALAGQKFFSLNCHLHMETSDFLGGLRPVRHTGTQQADAEDGRLFEWHDGPLILAMKQDGVFLMDEISLADDSVLERLNSVLEPEKSLVLAEKGSGDEEVELIIAGNKFRLVATMNPGGD-FGKKELSPALRNRFTEIW 1517 HSP 13 Score: 68.1662 bits (165), Expect = 2.884e-10 Identity = 44/136 (32.35%), Postives = 72/136 (52.94%), Query Frame = 2
Query: 458 TTGSNVYRLFRALQVSKKPLLLEGSPGVGKTSLVTQLAKLVGF----KVVRINLSEQTDANDLFGCFLPNENGKAGELSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPEINQKFMVEPGK 853
+T N+ RL A+ S+KP+LLEG G GKT+LV LA + G ++++ L +QTD+ L G + + G+ W+ G L QA+ G WILL++++ A V+ + +P + K + PG+
Sbjct: 294 STCQNLRRLAMAV-ASQKPILLEGPIGCGKTALVEYLAAVTGHVKAPDILKVQLGDQTDSKMLLGMYRCTD--VPGKFVWQPGSLTQAVSKGYWILLEDIDHAPLDVI-----------SVLLPLMENKKLTIPGR 415 HSP 14 Score: 63.5438 bits (153), Expect = 5.978e-9 Identity = 37/97 (38.14%), Postives = 60/97 (61.86%), Query Frame = 1
Query: 1570 FKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLE-PNGSLLLTERGLVYGDRIEEIKPHKNFRLFLILDP--DCGGE-ISKAMRNRGLEICI 1848
F +V+ TL QA+++G+W++LD +N +A L+ L+GLLE +GSL+L +RG E + H +FRLF ++P D G + +RNR E+ +
Sbjct: 793 FAFVEGTLAQAVKKGEWILLDEINLAAAETLECLSGLLEGSSGSLVLLDRG-----DTEPLFRHSDFRLFACMNPATDVGKRNLPLGIRNRFTELYV 884 HSP 15 Score: 55.4546 bits (132), Expect = 1.733e-6 Identity = 31/95 (32.63%), Postives = 52/95 (54.74%), Query Frame = 1
Query: 1417 VLVGPAGTGKTTIVKLLSDITGNHLKS-----MSMNSAMDTTDLLGSFEQQKEGGKFKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLE 1686
+L GP G GKT +V+ L+ +TG H+K+ + + D+ LLG + GKF W +L QA+ +G W++L+ ++ V+ L L+E
Sbjct: 313 LLEGPIGCGKTALVEYLAAVTG-HVKAPDILKVQLGDQTDSKMLLGMYRCTDVPGKFVWQPGSLTQAVSKGYWILLEDIDHAPLDVISVLLPLME 406 HSP 16 Score: 55.4546 bits (132), Expect = 1.777e-6 Identity = 35/98 (35.71%), Postives = 50/98 (51.02%), Query Frame = 2
Query: 671 GELSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPEINQKFMVEPGKT-----RLFACQNPVSEGSGRKGLPKSFVNRFTQVYM 949
G W DG L+QAL+AGDW+L+D +N S SVL+ LNA L+ G + I E P T RLF +P + ++ NR ++Y+
Sbjct: 2207 GGFEWLDGTLVQALQAGDWLLMDNVNFCSPSVLDRLNALLEPGGSLTINERGIIDGTTPTITPHPNFRLFLTMDPT-----HGEISRAMRNRGVEIYI 2299
BLAST of Midasin vs. Ensembl Xenopus
Match: MDN1 (midasin AAA ATPase 1 [Source:NCBI gene;Acc:100486293]) HSP 1 Score: 338.576 bits (867), Expect = 6.974e-114 Identity = 178/375 (47.47%), Postives = 243/375 (64.80%), Query Frame = 2
Query: 89 VVTIRDLLSWVNFINISIGNKTSL-----------YQSIFHGAYMVIIDSIND-KLNKSFYEE-----LLIKKLDYLVKSSFPDIVECLDF--QSSRFELSVKENKVGIDPFYIELGSHSDWXXXXXXXXXXKNFQYSFNSKTTGSNVYRLFRALQVSKKPLLLEGSPGVGKTSLVTQLAKLVGFKVVRINLSEQTDANDLFGCFLPNENGKAGELSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPEINQKFMVEPGKTRLFACQNPVSEGSGRKGLPKSFVNRFTQVYMSSLSDSDYRNILNCLYPAIARETIGFIVEFVQNLREHVVVMKKFGTKGSPWEFNLRDLCRWCDLMVKHQ 1156
++++RD+LSWVNF+N+ +G+K ++ + H A +V ID + +L+ + + L +KL ++ S D+ L Q+++ ++ +E+ GI PFYI +G + +K Y+ N++TT N RL RALQ++K P++LEGSPGVGKTSLVT LAK G +VRINLSEQTD DLFG LP E GK GE +WRDGPLL ALKAG WI+LDE+NLASQSVLE LNAC DHR E++IPE+ F V+ +T++F CQNP +G GRKGLP+SF+NRFTQVY+ LS +D I N L+P++ T+ +V F + V+V KK+G KG PWEFNLRDL RWC LM++ Q
Sbjct: 1547 ILSVRDILSWVNFMNVMVGDKDAVNSNAPGADLDAVTAFVHAACLVYIDGLGSGELSSATFARQQCMRFLREKLSEIL-SLTEDVHSELKLYDQATQRKICWREDCFGIHPFYIPIGPY---------FQENKITDYALNAETTSMNAQRLLRALQLNK-PIILEGSPGVGKTSLVTALAKASGNCLVRINLSEQTDVTDLFGTDLPVEGGKGGEFAWRDGPLLSALKAGHWIVLDELNLASQSVLEGLNACFDHRAEVYIPELGMNFQVQHERTKIFGCQNPFHQGGGRKGLPRSFLNRFTQVYVDQLSFADMEFIANSLFPSLGTSTVSKMVAFNNKIDHQVMVEKKWGQKGGPWEFNLRDLFRWCQLMLEDQ 1910 HSP 2 Score: 96.6709 bits (239), Expect = 6.974e-114 Identity = 57/132 (43.18%), Postives = 82/132 (62.12%), Query Frame = 1
Query: 1159 RTKEDQDACGKVYNELADKSRFARFIPSGLDFYITDQVLKIGQTQLSVNIANNTDNSKQFNKHL-LLAGQLAILESMMLTVDMGWLTVLVGPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGSFEQ 1551
RTK D++ VY E+ + ++ + FYIT Q +++G + L + D+ F+ HL LL L LE +M V MGW+ +LVGPA GKT++V+ L+ +TG+ LK M+MNSAMDTT+LLG FEQ
Sbjct: 1930 RTKPDREKIISVYKEVYGQD-LTPYLGTRY-FYITPQNVQLGYSVL----PRSCDSPLSFSGHLSLLHHSLQSLEPVMKCVQMGWMVILVGPAAVGKTSLVQTLALLTGHKLKIMAMNSAMDTTELLGGFEQ 2055 HSP 3 Score: 221.09 bits (562), Expect = 2.005e-56 Identity = 127/283 (44.88%), Postives = 187/283 (66.08%), Query Frame = 1
Query: 9145 WSQYESKTEYLSRQLCEMLRIVLEPTKANKLGGAYRTGKRLNMKKVIPYIASGFRKDKIWLRRNKPSQREYQVIIGVDNSKSMAYNVNHQLFFESLSIVLNGLTFLEVGQVGLCKFGAKAEVVLPLYEKFSSEIGDRLIETFDFSDKCSKFIPILEQSIKLFEENQNFLSSSAVRXXXXXXXXXXXXXXXXXXXXPKFISLIREAHNKGIFVVVILLDHADSKDSILNLKMVI--DDGC--DFVRFLDVFPLQNYTVLQDVTKLPTLLTDVLRQWFELSSSAD 9981
W +Y T LS+QLCE LR++LEPT+A KL G +RTGKRLNM+KVIPYIAS FRKDKIWLRR KPS+R+YQ+ + +D+S SM N + QL +ESL+++ N LT LEVGQ+ + FG +++ P +E+FS + G R++ F K + LE S+ +F Q LS S+ +T+QLLL++SDG + S ++ A N IF++ I+LD+ SKDS++++K+ I + G + +++ FP Y +L+DV LP L+D LRQWFEL ++++
Sbjct: 5050 WQRYLLLTAPLSQQLCEQLRLILEPTQAAKLKGDFRTGKRLNMRKVIPYIASQFRKDKIWLRRTKPSKRQYQICLAIDDSSSMVDNHSKQLAYESLAVIGNALTLLEVGQIAVYSFGESVKLLHPFHEQFSDQSGSRILSLCKFQQKKTLIAQFLESSVPMFAAAQQ-LSLSSNPETAQLLLIVSDGRGLFVEGKDRVTSAVQAARNANIFIIFIILDNPTSKDSVMDIKVPIFTEAGAMPKMISYMEQFPFPFYIILRDVNALPETLSDALRQWFELVTTSE 5331 HSP 4 Score: 137.117 bits (344), Expect = 4.757e-31 Identity = 94/247 (38.06%), Postives = 134/247 (54.25%), Query Frame = 1
Query: 1423 VGPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDT---------TDLLGSFEQQ-------KEGGKFKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGLVYGDRIEEIKPHKNFRLFLILDPDCGGEISKAMRNRGLEICIIPENRELTLTDNDLLAILITIYKLSVKNADSLLRFHKTMILAQSETPQIKIGN--------INSLIQAAKLIKDGYSLDQSLRSVYGDI 2091
VG AG+G TT LL+ + G ++ +N ++T + SF++Q + G F+WVD LVQAL+ GDWL++D VNFCS+SVLDRLN LLEP G L +TERG+ G + I PH NFRLFL +DP GEIS+AMRNRG+E+ I E+ + L DL I+ L V D +I SET + G+ +++ ++ ++ G L+ S RS ++
Sbjct: 2110 VGEAGSGITT--DLLNKLEGVLVQIQRLNLRLNTFTKTEFGALVEQFHSFKEQLLHSVEGQSSGTFEWVDGMLVQALQCGDWLLMDNVNFCSSSVLDRLNALLEPGGVLTMTERGVTDGSML-TITPHPNFRLFLSMDPS-HGEISRAMRNRGIELYISGESEGMLL---DLFDTKRLIHNLGVVGDDVC----NALIAVHSETKEAVTGSPCGSLSALLHAAALISQQLQRGIGLETSFRSACNEV 2345 HSP 5 Score: 134.806 bits (338), Expect = 2.906e-30 Identity = 110/384 (28.65%), Postives = 171/384 (44.53%), Query Frame = 2
Query: 23 VAGIIVRFLLIVQKMS--------HEINFSVVTIRDLLSWVNFINISIGNKTSLYQSIFHGAYMVIIDSINDKLNKSFYEELLIK-KLDYLVKSSFPDIVECLDFQSSRFELSVKENKVGIDPFYIELGSHSDWXXXXXXXXXXKNFQYSFNSKTTGSNVYRLFRALQVSKKPLLLEGSPGVGKTSLVTQLAKLVGFKVVRINLSEQTDANDLFGCFLPNENGKAGELSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPEINQKFMVEPGKTRLFACQNPVSEGSGRKGLPKSFVNRFTQVYMSSLSDSDYRNILN---CLYPAIARETIGFIVEFVQNLREHVVVMKKFGTKGSPWEFNLRDLCRWCD 1138
V GII +L I +K S H ++S+ T+ L + + N + SLY+ G ++ +D + + +++ + +L+ P+ Q + + V + ID YI YS N N+ L R + P+L++G VGKTSL+ LA G VRIN E TD + GC+ + +GK L +++G L+ A++ G W++LDE+NLA VLE LN LD E+FI E + P + LFA QNP GRK L ++F NRF +++ L ++ IL+ CL P+ + + ++E R V K G LRDL RW +
Sbjct: 882 VQGIISFYLAIRKKSSAELVDGTGHRPHYSLRTLCRALRYAS-ANPCNSVQRSLYEGFCLG-FLTQLDRASHSVVLGLIRRYIVQGNIKHLLSQPLPEPRGSRFVQIQGYWIPVGDKDPAIDESYI--------------------LTYSVNL-----NLRDLVRVVSAGTHPVLIQGETSVGKTSLIRWLACASGNHCVRINNHEHTDIQEYIGCYTSDVSGK---LIFKEGVLIDAMRKGYWVILDELNLAPTDVLEALNRLLDDNRELFITETQEVVKAHP-RFMLFATQNPPGLYGGRKVLSRAFRNRFVELHFDELPSAELETILHKRCCLPPSYCNKLVAVMLELQSYRRGSSVFAGKHGF------ITLRDLFRWAE 1228 HSP 6 Score: 95.5153 bits (236), Expect = 1.826e-18 Identity = 90/313 (28.75%), Postives = 148/313 (47.28%), Query Frame = 1
Query: 5176 NSSHVEEKQDTTVFRDCYRFAAALISSDNQGIPSD-DCKLFAANLSALNWEYETLSPGVPQNTIGR---PHDIYQDPIDVDNLLQAQKILPNANVSVTKLLGIYPEHTTXXXXXXXXXXXXXXSGDYSLIVFINNVERLIHEIQQWEVVAARHVSLIQHVDELNGLLVSWRHQELTMWENTISTANWQLERAVSREFFNHYNLIVQESNKIQYDNDFESINLILEPILNSLL-NSTVGNFEYRLKTMKLSIES---LISNREIDYRFKDRLKNILLNHYDFYRLYLGNVQEHLSSEYKRIGKELRDFIKTKRW 6090
N + K + F CY+ +AALI + S+ + L + L A + ++S N I + P+D YQ P ++ + Q QKIL N ++ KLL +PEH L+ + I I + L +N +E L+ + Q WE A R +SL +H+DE+ L++ WR EL W + A + + + +F+ Y L+ E + + E + L L + + ST+G F+ RL T+ L LI KD L ++L N Y +Y+ + VQ ++ + KEL++F+K RW
Sbjct: 3496 NPPEHQAKHYLSAFLSCYQTSAALIEHLYPLMGSELNDHLLGSQLLAGSLLQNSISGNTATNLILQHEGPYDFYQHP-NIPQVQQCQKILQNFTSAINKLLEEWPEHPALVQVLVVIERIQSFPLSSPLSKILNGLEILLAKAQDWEENANRGLSLRKHLDEITQLIIQWRKLELNCWSLGLDNALARHVQKTTSHWFSVYQLV--EKHLQEEHEQEELLLQSLTKTLQAFIEGSTLGEFQSRL-TLLLVFHCHVLLIPQSPK----KDSLCSVLWNLYHYYKQFASTVQARITELRNPLEKELKEFVKISRW 3800 HSP 7 Score: 92.4337 bits (228), Expect = 1.954e-17 Identity = 75/258 (29.07%), Postives = 131/258 (50.78%), Query Frame = 2
Query: 455 KTTGSNVYRLFRALQVS-KKPLLLEGSPGVGKTSLVTQLAKLVGFK----VVRINLSEQTDANDLFGCFLPNENGKAGELSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPEINQKFMVEPGKTRLFACQNPVSEGSGRKGLPK----SFVNR-FTQVYMSSLSDSDYRNILNCLYPAIARETIGFIVEFVQNLREHVVVMKKFGTKGS-----PWEFN------------LRDLCRWCDLMV 1147
++T N++ L AL VS + P+LLEG G GKT+LV LA + G ++++ L +QTD+ L G + + GE W+ G L QA+ G WILL++++ A V+ L L++ GE+ +P V PG +LFA + +S SG P+ S +++ +T++++ ++S + + ++ LYP + T + ++Q + + M T+ P E N LRDL +WC+ ++
Sbjct: 290 ESTCKNLHSL--ALAVSYQNPVLLEGPIGCGKTALVEYLATVTGRHQPPDILKVQLGDQTDSKMLLGMYRCTD--IPGEFVWQPGTLTQAVANGHWILLEDIDYAPLDVISVLLPVLEN-GELLMPGRGDCIKVAPG-FQLFATRRLLSSSSGVWYRPQNTHASLLDKHWTRIHLGNMSREELKEVILKLYPNLELVTDRLLDIYIQLTGDKHLAMSDLETEAEALLQKPLEQNVPRPNLEGRGLSLRDLLKWCNRII 541 HSP 8 Score: 82.0333 bits (201), Expect = 2.202e-14 Identity = 58/178 (32.58%), Postives = 92/178 (51.69%), Query Frame = 1
Query: 1363 QLAILESMMLTVDMGWLTVLVGPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGSFEQQKEGGK----------FKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGLVYGDRIEEIK---PHKNFRLFLILDPDCGG-----EISKAMRNRGLEI 1842
+LA+L L G +LVG G GKTTI +L + + L ++ + M+T+D LG ++ K F+W D LV A+++ + ++D ++ SVL+RLN +LE +L+L E+G D EE++ K FR+ ++P GG E+S A+RNR EI
Sbjct: 1319 RLAVLVGRAL--QFGEPVLLVGDTGCGKTTICQLFAALASQTLHCVNCHQHMETSDFLGGLRPIRQRSKDKDDVEYSKLFEWHDGPLVLAIKEDGFFLMDEISLADDSVLERLNSVLEVEKTLVLAEKG-SGEDETEEVEILTAGKQFRILATMNP--GGDFGKKELSPALRNRFTEI 1491 HSP 9 Score: 79.7221 bits (195), Expect = 1.268e-13 Identity = 67/207 (32.37%), Postives = 116/207 (56.04%), Query Frame = 1
Query: 7417 INALEQSLDTQKILDLFTKIKSFFCDLNMNQVNKFRNLINEMV--LPKLRSLEKVEKEFLGLLLNYHRVLISATDALLTVTTKLMKEGFCIPNDSAWNNEAENSNPSEKMNDFEMAGMDGGVGTNDVTDEIESESQIE-----GNNKDKKDETENQPDIPDEDDGIEMTDDFNGKMHDPEIXXXXXXXXXXXXXXXXXXXMGEVGNA 8016
+ +L+ L + +L ++K++ D ++ F +V LP L + + +L L L HR L + T+L ++GFC+P + EA +E +D+E G+ G G DV+D+IE+E QIE G +K+K D+ N+PD+ +ED+ IEM++DF+GK+HD E+ + +E D +N + +L+ +MG++GNA
Sbjct: 4368 LKSLQVHLIISAVSELLERLKTYGEDCIKDKYTFFNQCCGLLVRLLPMLSTYSDLILFYLSLSLATHRSTGKLFSILAHLFTELAQKGFCLPKELI--EEAAGEGATE-FHDYEGGGIGEGEGMKDVSDKIENEDQIEDTLKKGEDKEK-DDPNNKPDVKEEDNAIEMSEDFDGKIHDGELQKKEEEDESNSDEEELDKQMGDLGNA 4570 HSP 10 Score: 79.337 bits (194), Expect = 1.499e-13 Identity = 51/152 (33.55%), Postives = 79/152 (51.97%), Query Frame = 2
Query: 689 DGPLLQALKAGDWILLDEMNLASQSVLECLNACLD--HRGEIFIPEINQKFMVEPGKTRLFACQNPVSEGSGRKGLPKSFVNRFTQVYMSSLSDSDYRNILNCLYPA---IARETIGFIVEFVQNLREHVVVMKKFGTKGSPWEFNLRDLCR 1129
+G L QA+K G+WILLDE+NLA+ LECL L+ + + + + +V RLFAC NP ++ G++ LP NRFT++Y+ L + IL Y +++ + I+ F +R+ GT P ++LR LCR
Sbjct: 768 EGTLAQAVKKGEWILLDEINLAAAETLECLCGLLEGISGSLVLLDRGDTEPLVRHPDFRLFACMNPATD-VGKRNLPPGIRNRFTELYVEELENEGDLQILIMDYLRGLNVSKTVVQGIISFYLAIRKKSSAELVDGTGHRP-HYSLRTLCR 917 HSP 11 Score: 76.2554 bits (186), Expect = 1.279e-12 Identity = 51/161 (31.68%), Postives = 82/161 (50.93%), Query Frame = 1
Query: 1375 LESMMLTVDMGWLTVLV-GPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGSFEQQKEGGKFKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGLVYGDRIEEIKPHKNFRLFLILDPD--CGGE--ISKAMRNRGLEI 1842
L ++ V G VL+ G GKT++++ L+ +GNH ++ + D + +G + GK + + L+ A+R+G W+ILD +N VL+ LN LL+ N L +TE E +K H F LF +P GG +S+A RNR +E+
Sbjct: 1014 LRDLVRVVSAGTHPVLIQGETSVGKTSLIRWLACASGNHCVRINNHEHTDIQEYIGCYTSDV-SGKLIFKEGVLIDAMRKGYWVILDELNLAPTDVLEALNRLLDDNRELFITETQ-------EVVKAHPRFMLFATQNPPGLYGGRKVLSRAFRNRFVEL 1166 HSP 12 Score: 75.485 bits (184), Expect = 2.242e-12 Identity = 39/114 (34.21%), Postives = 64/114 (56.14%), Query Frame = 1
Query: 1417 VLVGPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGS--FEQQKEGGKFKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGLVYGDRIEEIK 1752
+L G G GKT++V L+ +GN L ++++ D TDL G+ + +GG+F W D L+ AL+ G W++LD +N S SVL+ LN + + + E G+ + + E K
Sbjct: 1698 ILEGSPGVGKTSLVTALAKASGNCLVRINLSEQTDVTDLFGTDLPVEGGKGGEFAWRDGPLLSALKAGHWIVLDELNLASQSVLEGLNACFDHRAEVYIPELGMNFQVQHERTK 1811 HSP 13 Score: 71.633 bits (174), Expect = 3.381e-11 Identity = 53/175 (30.29%), Postives = 85/175 (48.57%), Query Frame = 2
Query: 488 RALQVSKKPLLLEGSPGVGKTSLVTQLAKLVGFKVVRINLSEQTDANDLFGCFLP-NENGKAGE-------LSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIP-------EINQKFMVEPGKT-RLFACQNPVSEGSGRKGLPKSFVNRFTQVYMSSLSD 964
RALQ + P+LL G G GKT++ A L + +N + + +D G P + K + W DGPL+ A+K + L+DE++LA SVLE LN+ L+ + + E + ++ GK R+ A NP + G+K L + NRFT+++ +D
Sbjct: 1326 RALQFGE-PVLLVGDTGCGKTTICQLFAALASQTLHCVNCHQHMETSDFLGGLRPIRQRSKDKDDVEYSKLFEWHDGPLVLAIKEDGFFLMDEISLADDSVLERLNSVLEVEKTLVLAEKGSGEDETEEVEILTAGKQFRILATMNPGGD-FGKKELSPALRNRFTEIWCPQSND 1498 HSP 14 Score: 69.707 bits (169), Expect = 1.324e-10 Identity = 47/155 (30.32%), Postives = 77/155 (49.68%), Query Frame = 1
Query: 1324 NSKQFNKHLLLAGQLAILESMMLTVDMGWLTVLVGPAGTGKTTIVKLLSDITGNH----LKSMSMNSAMDTTDLLGSFEQQKEGGKFKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGLVYGDRIEEIKPHKNFRLF 1776
+S+ ++ +L+ L S+ L V +L GP G GKT +V+ L+ +TG H + + + D+ LLG + G+F W TL QA+ G W++L+ +++ V+ L +LE NG LL+ RG + IK F+LF
Sbjct: 279 HSESISQLVLVESTCKNLHSLALAVSYQNPVLLEGPIGCGKTALVEYLATVTGRHQPPDILKVQLGDQTDSKMLLGMYRCTDIPGEFVWQPGTLTQAVANGHWILLEDIDYAPLDVISVLLPVLE-NGELLMPGRG-------DCIKVAPGFQLF 425 HSP 15 Score: 63.5438 bits (153), Expect = 9.169e-9 Identity = 37/97 (38.14%), Postives = 59/97 (60.82%), Query Frame = 1
Query: 1570 FKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEP-NGSLLLTERGLVYGDRIEEIKPHKNFRLFLILDP--DCGGE-ISKAMRNRGLEICI 1848
F +V+ TL QA+++G+W++LD +N +A L+ L GLLE +GSL+L +RG E + H +FRLF ++P D G + +RNR E+ +
Sbjct: 764 FAFVEGTLAQAVKKGEWILLDEINLAAAETLECLCGLLEGISGSLVLLDRG-----DTEPLVRHPDFRLFACMNPATDVGKRNLPPGIRNRFTELYV 855 HSP 16 Score: 58.9214 bits (141), Expect = 2.050e-7 Identity = 48/163 (29.45%), Postives = 74/163 (45.40%), Query Frame = 2
Query: 527 GSPGVGKTSLVTQLAKLVGFKVVRINLSEQTDANDLFGCFLPN------------ENGKAGELSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPEI----NQKFMVEPGKT-RLFACQNPVSEGSGRKGLPKSFVNRFTQVYMSSLSD 964
G G G T+ + + V ++ R+NL T FG + E +G W DG L+QAL+ GDW+L+D +N S SVL+ LNA L+ G + + E + P RLF +P S G + ++ NR ++Y+S S+
Sbjct: 2111 GEAGSGITTDLLNKLEGVLVQIQRLNLRLNTFTKTEFGALVEQFHSFKEQLLHSVEGQSSGTFEWVDGMLVQALQCGDWLLMDNVNFCSSSVLDRLNALLEPGGVLTMTERGVTDGSMLTITPHPNFRLFLSMDP-SHGE----ISRAMRNRGIELYISGESE 2268
BLAST of Midasin vs. Ensembl Xenopus
Match: MDN1 (midasin AAA ATPase 1 [Source:NCBI gene;Acc:100486293]) HSP 1 Score: 338.191 bits (866), Expect = 7.579e-114 Identity = 178/375 (47.47%), Postives = 243/375 (64.80%), Query Frame = 2
Query: 89 VVTIRDLLSWVNFINISIGNKTSL-----------YQSIFHGAYMVIIDSIND-KLNKSFYEE-----LLIKKLDYLVKSSFPDIVECLDF--QSSRFELSVKENKVGIDPFYIELGSHSDWXXXXXXXXXXKNFQYSFNSKTTGSNVYRLFRALQVSKKPLLLEGSPGVGKTSLVTQLAKLVGFKVVRINLSEQTDANDLFGCFLPNENGKAGELSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPEINQKFMVEPGKTRLFACQNPVSEGSGRKGLPKSFVNRFTQVYMSSLSDSDYRNILNCLYPAIARETIGFIVEFVQNLREHVVVMKKFGTKGSPWEFNLRDLCRWCDLMVKHQ 1156
++++RD+LSWVNF+N+ +G+K ++ + H A +V ID + +L+ + + L +KL ++ S D+ L Q+++ ++ +E+ GI PFYI +G + +K Y+ N++TT N RL RALQ++K P++LEGSPGVGKTSLVT LAK G +VRINLSEQTD DLFG LP E GK GE +WRDGPLL ALKAG WI+LDE+NLASQSVLE LNAC DHR E++IPE+ F V+ +T++F CQNP +G GRKGLP+SF+NRFTQVY+ LS +D I N L+P++ T+ +V F + V+V KK+G KG PWEFNLRDL RWC LM++ Q
Sbjct: 1513 ILSVRDILSWVNFMNVMVGDKDAVNSNAPGADLDAVTAFVHAACLVYIDGLGSGELSSATFARQQCMRFLREKLSEIL-SLTEDVHSELKLYDQATQRKICWREDCFGIHPFYIPIGPY---------FQENKITDYALNAETTSMNAQRLLRALQLNK-PIILEGSPGVGKTSLVTALAKASGNCLVRINLSEQTDVTDLFGTDLPVEGGKGGEFAWRDGPLLSALKAGHWIVLDELNLASQSVLEGLNACFDHRAEVYIPELGMNFQVQHERTKIFGCQNPFHQGGGRKGLPRSFLNRFTQVYVDQLSFADMEFIANSLFPSLGTSTVSKMVAFNNKIDHQVMVEKKWGQKGGPWEFNLRDLFRWCQLMLEDQ 1876 HSP 2 Score: 97.0561 bits (240), Expect = 7.579e-114 Identity = 57/132 (43.18%), Postives = 82/132 (62.12%), Query Frame = 1
Query: 1159 RTKEDQDACGKVYNELADKSRFARFIPSGLDFYITDQVLKIGQTQLSVNIANNTDNSKQFNKHL-LLAGQLAILESMMLTVDMGWLTVLVGPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGSFEQ 1551
RTK D++ VY E+ + ++ + FYIT Q +++G + L + D+ F+ HL LL L LE +M V MGW+ +LVGPA GKT++V+ L+ +TG+ LK M+MNSAMDTT+LLG FEQ
Sbjct: 1896 RTKPDREKIISVYKEVYGQD-LTPYLGTRY-FYITPQNVQLGYSVL----PRSCDSPLSFSGHLSLLHHSLQSLEPVMKCVQMGWMVILVGPAAVGKTSLVQTLALLTGHKLKIMAMNSAMDTTELLGGFEQ 2021 HSP 3 Score: 220.705 bits (561), Expect = 2.542e-56 Identity = 127/283 (44.88%), Postives = 187/283 (66.08%), Query Frame = 1
Query: 9145 WSQYESKTEYLSRQLCEMLRIVLEPTKANKLGGAYRTGKRLNMKKVIPYIASGFRKDKIWLRRNKPSQREYQVIIGVDNSKSMAYNVNHQLFFESLSIVLNGLTFLEVGQVGLCKFGAKAEVVLPLYEKFSSEIGDRLIETFDFSDKCSKFIPILEQSIKLFEENQNFLSSSAVRXXXXXXXXXXXXXXXXXXXXPKFISLIREAHNKGIFVVVILLDHADSKDSILNLKMVI--DDGC--DFVRFLDVFPLQNYTVLQDVTKLPTLLTDVLRQWFELSSSAD 9981
W +Y T LS+QLCE LR++LEPT+A KL G +RTGKRLNM+KVIPYIAS FRKDKIWLRR KPS+R+YQ+ + +D+S SM N + QL +ESL+++ N LT LEVGQ+ + FG +++ P +E+FS + G R++ F K + LE S+ +F Q LS S+ +T+QLLL++SDG + S ++ A N IF++ I+LD+ SKDS++++K+ I + G + +++ FP Y +L+DV LP L+D LRQWFEL ++++
Sbjct: 4926 WQRYLLLTAPLSQQLCEQLRLILEPTQAAKLKGDFRTGKRLNMRKVIPYIASQFRKDKIWLRRTKPSKRQYQICLAIDDSSSMVDNHSKQLAYESLAVIGNALTLLEVGQIAVYSFGESVKLLHPFHEQFSDQSGSRILSLCKFQQKKTLIAQFLESSVPMFAAAQQ-LSLSSNPETAQLLLIVSDGRGLFVEGKDRVTSAVQAARNANIFIIFIILDNPTSKDSVMDIKVPIFTEAGAMPKMISYMEQFPFPFYIILRDVNALPETLSDALRQWFELVTTSE 5207 HSP 4 Score: 137.117 bits (344), Expect = 5.113e-31 Identity = 94/247 (38.06%), Postives = 134/247 (54.25%), Query Frame = 1
Query: 1423 VGPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDT---------TDLLGSFEQQ-------KEGGKFKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGLVYGDRIEEIKPHKNFRLFLILDPDCGGEISKAMRNRGLEICIIPENRELTLTDNDLLAILITIYKLSVKNADSLLRFHKTMILAQSETPQIKIGN--------INSLIQAAKLIKDGYSLDQSLRSVYGDI 2091
VG AG+G TT LL+ + G ++ +N ++T + SF++Q + G F+WVD LVQAL+ GDWL++D VNFCS+SVLDRLN LLEP G L +TERG+ G + I PH NFRLFL +DP GEIS+AMRNRG+E+ I E+ + L DL I+ L V D +I SET + G+ +++ ++ ++ G L+ S RS ++
Sbjct: 2076 VGEAGSGITT--DLLNKLEGVLVQIQRLNLRLNTFTKTEFGALVEQFHSFKEQLLHSVEGQSSGTFEWVDGMLVQALQCGDWLLMDNVNFCSSSVLDRLNALLEPGGVLTMTERGVTDGSML-TITPHPNFRLFLSMDPS-HGEISRAMRNRGIELYISGESEGMLL---DLFDTKRLIHNLGVVGDDVC----NALIAVHSETKEAVTGSPCGSLSALLHAAALISQQLQRGIGLETSFRSACNEV 2311 HSP 5 Score: 134.806 bits (338), Expect = 2.778e-30 Identity = 110/384 (28.65%), Postives = 171/384 (44.53%), Query Frame = 2
Query: 23 VAGIIVRFLLIVQKMS--------HEINFSVVTIRDLLSWVNFINISIGNKTSLYQSIFHGAYMVIIDSINDKLNKSFYEELLIK-KLDYLVKSSFPDIVECLDFQSSRFELSVKENKVGIDPFYIELGSHSDWXXXXXXXXXXKNFQYSFNSKTTGSNVYRLFRALQVSKKPLLLEGSPGVGKTSLVTQLAKLVGFKVVRINLSEQTDANDLFGCFLPNENGKAGELSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPEINQKFMVEPGKTRLFACQNPVSEGSGRKGLPKSFVNRFTQVYMSSLSDSDYRNILN---CLYPAIARETIGFIVEFVQNLREHVVVMKKFGTKGSPWEFNLRDLCRWCD 1138
V GII +L I +K S H ++S+ T+ L + + N + SLY+ G ++ +D + + +++ + +L+ P+ Q + + V + ID YI YS N N+ L R + P+L++G VGKTSL+ LA G VRIN E TD + GC+ + +GK L +++G L+ A++ G W++LDE+NLA VLE LN LD E+FI E + P + LFA QNP GRK L ++F NRF +++ L ++ IL+ CL P+ + + ++E R V K G LRDL RW +
Sbjct: 848 VQGIISFYLAIRKKSSAELVDGTGHRPHYSLRTLCRALRYAS-ANPCNSVQRSLYEGFCLG-FLTQLDRASHSVVLGLIRRYIVQGNIKHLLSQPLPEPRGSRFVQIQGYWIPVGDKDPAIDESYI--------------------LTYSVNL-----NLRDLVRVVSAGTHPVLIQGETSVGKTSLIRWLACASGNHCVRINNHEHTDIQEYIGCYTSDVSGK---LIFKEGVLIDAMRKGYWVILDELNLAPTDVLEALNRLLDDNRELFITETQEVVKAHP-RFMLFATQNPPGLYGGRKVLSRAFRNRFVELHFDELPSAELETILHKRCCLPPSYCNKLVAVMLELQSYRRGSSVFAGKHGF------ITLRDLFRWAE 1194 HSP 6 Score: 108.612 bits (270), Expect = 2.516e-22 Identity = 98/348 (28.16%), Postives = 165/348 (47.41%), Query Frame = 1
Query: 5176 NSSHVEEKQDTTVFRDCYRFAAALISSDNQGIPSD-DCKLFAANLSALNWEYETLSPGVPQNTIGR---PHDIYQDPIDVDNLLQAQKILPNANVSVTKLLGIYPEHTTXXXXXXXXXXXXXXSGDYSLIVFINNVERLIHEIQQWEVVAARHVSLIQHVDELNGLLVSWRHQELTMWENTISTANWQLERAVSREFFNHYNLIVQESNKIQYDNDFESINLILEPILNSLL-NSTVGNFEYRLKTMKLSIES---LISNREIDYRFKDRLKNILLNHYDFYRLYLGNVQEHLSSEYKRIGKELRDFIKTKRWGKTP--PKITDVLKCHKIVFKYKRQWSGIINQPCT 6189
N + K + F CY+ +AALI + S+ + L + L A + ++S N I + P+D YQ P ++ + Q QKIL N ++ KLL +PEH L+ + I I + L +N +E L+ + Q WE A R +SL +H+DE+ L++ WR EL W + A + + + +F+ Y L+ E + + E + L L + + ST+G F+ RL T+ L LI KD L ++L N Y +Y+ + VQ ++ + KEL++F+K RW V K H+ +FKY +++ ++ +PC+
Sbjct: 3474 NPPEHQAKHYLSAFLSCYQTSAALIEHLYPLMGSELNDHLLGSQLLAGSLLQNSISGNTATNLILQHEGPYDFYQHP-NIPQVQQCQKILQNFTSAINKLLEEWPEHPALVQVLVVIERIQSFPLSSPLSKILNGLEILLAKAQDWEENANRGLSLRKHLDEITQLIIQWRKLELNCWSLGLDNALARHVQKTTSHWFSVYQLV--EKHLQEEHEQEELLLQSLTKTLQAFIEGSTLGEFQSRL-TLLLVFHCHVLLIPQSPK----KDSLCSVLWNLYHYYKQFASTVQARITELRNPLEKELKEFVKISRWNDVSFWSVKQSVDKTHRTLFKYMKKFETVLCEPCS 3813 HSP 7 Score: 92.4337 bits (228), Expect = 1.855e-17 Identity = 75/258 (29.07%), Postives = 131/258 (50.78%), Query Frame = 2
Query: 455 KTTGSNVYRLFRALQVS-KKPLLLEGSPGVGKTSLVTQLAKLVGFK----VVRINLSEQTDANDLFGCFLPNENGKAGELSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPEINQKFMVEPGKTRLFACQNPVSEGSGRKGLPK----SFVNR-FTQVYMSSLSDSDYRNILNCLYPAIARETIGFIVEFVQNLREHVVVMKKFGTKGS-----PWEFN------------LRDLCRWCDLMV 1147
++T N++ L AL VS + P+LLEG G GKT+LV LA + G ++++ L +QTD+ L G + + GE W+ G L QA+ G WILL++++ A V+ L L++ GE+ +P V PG +LFA + +S SG P+ S +++ +T++++ ++S + + ++ LYP + T + ++Q + + M T+ P E N LRDL +WC+ ++
Sbjct: 283 ESTCKNLHSL--ALAVSYQNPVLLEGPIGCGKTALVEYLATVTGRHQPPDILKVQLGDQTDSKMLLGMYRCTD--IPGEFVWQPGTLTQAVANGHWILLEDIDYAPLDVISVLLPVLEN-GELLMPGRGDCIKVAPG-FQLFATRRLLSSSSGVWYRPQNTHASLLDKHWTRIHLGNMSREELKEVILKLYPNLELVTDRLLDIYIQLTGDKHLAMSDLETEAEALLQKPLEQNVPRPNLEGRGLSLRDLLKWCNRII 534 HSP 8 Score: 82.0333 bits (201), Expect = 2.273e-14 Identity = 58/178 (32.58%), Postives = 92/178 (51.69%), Query Frame = 1
Query: 1363 QLAILESMMLTVDMGWLTVLVGPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGSFEQQKEGGK----------FKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGLVYGDRIEEIK---PHKNFRLFLILDPDCGG-----EISKAMRNRGLEI 1842
+LA+L L G +LVG G GKTTI +L + + L ++ + M+T+D LG ++ K F+W D LV A+++ + ++D ++ SVL+RLN +LE +L+L E+G D EE++ K FR+ ++P GG E+S A+RNR EI
Sbjct: 1285 RLAVLVGRAL--QFGEPVLLVGDTGCGKTTICQLFAALASQTLHCVNCHQHMETSDFLGGLRPIRQRSKDKDDVEYSKLFEWHDGPLVLAIKEDGFFLMDEISLADDSVLERLNSVLEVEKTLVLAEKG-SGEDETEEVEILTAGKQFRILATMNP--GGDFGKKELSPALRNRFTEI 1457 HSP 9 Score: 81.6481 bits (200), Expect = 3.205e-14 Identity = 135/521 (25.91%), Postives = 234/521 (44.91%), Query Frame = 1
Query: 7417 INALEQSLDTQKILDLFTKIKSFFCDLNMNQVNKFRNLINEMV--LPKLRSLEKVEKEFLGLLLNYHRVLISATDALLTVTTKLMKEGFCIPNDSAWNNEAENSNPSEKMNDFEMAGMDGGVGTNDVTDEIESESQIE-----GNNKDKKDETENQPDIPDEDDGIEMTDDFNGKMHDPEIXXXXXXXXXXXXXXXXXXXMGEVGNAP--ETDVNXXXXXXXXXXXXXXXXXXXXXXXXGGVSEQNTTEMVA-DEKEDKDHXXXXXXXXXXXXXXXXXXXXXXTDAGNEEDIGEFKDDEDSKDQENAPENTGQEPEKLDNTEEENNPLDDFWNDECQLNVESDSDQVENNDIPD------------VEEKEE-NIDDLQPIDETPEDKEEVISGKVEQEFNMAGGYEINENHEMTLDNENVEENTEIXXXXXXXXXXXXXXXXXAEGNIDNNEPKFDNKESSDKKQDVSSKPSEN---RSLGDVNQTFKRKAEILDENN--ADDNKRPR----TTDAFSHI 8883
+ +L+ L + +L ++K++ D ++ F +V LP L + + +L L L HR L + T+L ++GFC+P + EA +E +D+E G+ G G DV+D+IE+E QIE G +K+K D+ N+PD+ +ED+ IEM++DF+GK+HD E+ + +E D +N + +L+ +MG++GNA + D D++ +++ +++ ++ + G + +E+VA D+ D D + E+ E +D D + E+ P N Q E++D E + N D + + + + + E D+PD +EKEE N + TPE KEE I+ E+ + + G EN L +E+ E + K D KE D+ V KP ++ RSLGD +Q ++ I+D ++ D + RP DA+ HI
Sbjct: 4372 LKSLQVHLIISAVSELLERLKTYGEDCIKDKYTFFNQCCGLLVRLLPMLSTYSDLILFYLSLSLATHRSTGKLFSILAHLFTELAQKGFCLPKELI--EEAAGEGATE-FHDYEGGGIGEGEGMKDVSDKIENEDQIEDTLKKGEDKEK-DDPNNKPDVKEEDNAIEMSEDFDGKIHDGELQKKEEEDESNSDEEELDKQMGDLGNAEADKLDERLWGEDDDDDDDDENEDESDKTEETGPGMDMGESELVAKDDNLDAD------------------------NKSKEDKKQEVRDKRDEEQAEDVP-NKQQIHEQIDEREYDENETDPYHGN------QENKPEPEALDLPDNLNLENEEEKNSGDEKEEGNEGNYMSYFATPE-KEEGIADSTERMEHDSCGQTAEEN----LQSESAAELA------------------------GSASEKDDAKEVRDRTY-VKRKPGQSDDERSLGDHSQQVHKRLRIMDSSSEMTDSSDRPEHKSGQADAYEHI 4827 HSP 10 Score: 79.7221 bits (195), Expect = 1.353e-13 Identity = 52/157 (33.12%), Postives = 80/157 (50.96%), Query Frame = 2
Query: 689 DGPLLQALKAGDWILLDEMNLASQSVLECLNACLD--HRGEIFIPEINQKFMVEPGKTRLFACQNPVSEGSGRKGLPKSFVNRFTQVYMSSLSDS--------DYRNILNCLYPAIARETIGFIVEFVQNLREHVVVMKKFGTKGSPWEFNLRDLCR 1129
+G L QA+K G+WILLDE+NLA+ LECL L+ + + + + +V RLFAC NP ++ G++ LP NRFT++Y+ L + DY LN +++ + I+ F +R+ GT P ++LR LCR
Sbjct: 734 EGTLAQAVKKGEWILLDEINLAAAETLECLCGLLEGISGSLVLLDRGDTEPLVRHPDFRLFACMNPATD-VGKRNLPPGIRNRFTELYVEELENEGDLQILIMDYLRGLN-----VSKTVVQGIISFYLAIRKKSSAELVDGTGHRP-HYSLRTLCR 883 HSP 11 Score: 76.2554 bits (186), Expect = 1.267e-12 Identity = 51/161 (31.68%), Postives = 82/161 (50.93%), Query Frame = 1
Query: 1375 LESMMLTVDMGWLTVLV-GPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGSFEQQKEGGKFKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGLVYGDRIEEIKPHKNFRLFLILDPD--CGGE--ISKAMRNRGLEI 1842
L ++ V G VL+ G GKT++++ L+ +GNH ++ + D + +G + GK + + L+ A+R+G W+ILD +N VL+ LN LL+ N L +TE E +K H F LF +P GG +S+A RNR +E+
Sbjct: 980 LRDLVRVVSAGTHPVLIQGETSVGKTSLIRWLACASGNHCVRINNHEHTDIQEYIGCYTSDV-SGKLIFKEGVLIDAMRKGYWVILDELNLAPTDVLEALNRLLDDNRELFITETQ-------EVVKAHPRFMLFATQNPPGLYGGRKVLSRAFRNRFVEL 1132 HSP 12 Score: 75.485 bits (184), Expect = 2.220e-12 Identity = 39/114 (34.21%), Postives = 64/114 (56.14%), Query Frame = 1
Query: 1417 VLVGPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGS--FEQQKEGGKFKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGLVYGDRIEEIK 1752
+L G G GKT++V L+ +GN L ++++ D TDL G+ + +GG+F W D L+ AL+ G W++LD +N S SVL+ LN + + + E G+ + + E K
Sbjct: 1664 ILEGSPGVGKTSLVTALAKASGNCLVRINLSEQTDVTDLFGTDLPVEGGKGGEFAWRDGPLLSALKAGHWIVLDELNLASQSVLEGLNACFDHRAEVYIPELGMNFQVQHERTK 1777 HSP 13 Score: 71.633 bits (174), Expect = 3.105e-11 Identity = 53/175 (30.29%), Postives = 85/175 (48.57%), Query Frame = 2
Query: 488 RALQVSKKPLLLEGSPGVGKTSLVTQLAKLVGFKVVRINLSEQTDANDLFGCFLP-NENGKAGE-------LSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIP-------EINQKFMVEPGKT-RLFACQNPVSEGSGRKGLPKSFVNRFTQVYMSSLSD 964
RALQ + P+LL G G GKT++ A L + +N + + +D G P + K + W DGPL+ A+K + L+DE++LA SVLE LN+ L+ + + E + ++ GK R+ A NP + G+K L + NRFT+++ +D
Sbjct: 1292 RALQFGE-PVLLVGDTGCGKTTICQLFAALASQTLHCVNCHQHMETSDFLGGLRPIRQRSKDKDDVEYSKLFEWHDGPLVLAIKEDGFFLMDEISLADDSVLERLNSVLEVEKTLVLAEKGSGEDETEEVEILTAGKQFRILATMNPGGD-FGKKELSPALRNRFTEIWCPQSND 1464 HSP 14 Score: 68.9366 bits (167), Expect = 2.044e-10 Identity = 45/138 (32.61%), Postives = 69/138 (50.00%), Query Frame = 1
Query: 1375 LESMMLTVDMGWLTVLVGPAGTGKTTIVKLLSDITGNH----LKSMSMNSAMDTTDLLGSFEQQKEGGKFKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGLVYGDRIEEIKPHKNFRLF 1776
L S+ L V +L GP G GKT +V+ L+ +TG H + + + D+ LLG + G+F W TL QA+ G W++L+ +++ V+ L +LE NG LL+ RG + IK F+LF
Sbjct: 289 LHSLALAVSYQNPVLLEGPIGCGKTALVEYLATVTGRHQPPDILKVQLGDQTDSKMLLGMYRCTDIPGEFVWQPGTLTQAVANGHWILLEDIDYAPLDVISVLLPVLE-NGELLMPGRG-------DCIKVAPGFQLF 418 HSP 15 Score: 63.5438 bits (153), Expect = 9.470e-9 Identity = 37/97 (38.14%), Postives = 59/97 (60.82%), Query Frame = 1
Query: 1570 FKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEP-NGSLLLTERGLVYGDRIEEIKPHKNFRLFLILDP--DCGGE-ISKAMRNRGLEICI 1848
F +V+ TL QA+++G+W++LD +N +A L+ L GLLE +GSL+L +RG E + H +FRLF ++P D G + +RNR E+ +
Sbjct: 730 FAFVEGTLAQAVKKGEWILLDEINLAAAETLECLCGLLEGISGSLVLLDRG-----DTEPLVRHPDFRLFACMNPATDVGKRNLPPGIRNRFTELYV 821 HSP 16 Score: 59.3066 bits (142), Expect = 2.013e-7 Identity = 48/163 (29.45%), Postives = 74/163 (45.40%), Query Frame = 2
Query: 527 GSPGVGKTSLVTQLAKLVGFKVVRINLSEQTDANDLFGCFLPN------------ENGKAGELSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPEI----NQKFMVEPGKT-RLFACQNPVSEGSGRKGLPKSFVNRFTQVYMSSLSD 964
G G G T+ + + V ++ R+NL T FG + E +G W DG L+QAL+ GDW+L+D +N S SVL+ LNA L+ G + + E + P RLF +P S G + ++ NR ++Y+S S+
Sbjct: 2077 GEAGSGITTDLLNKLEGVLVQIQRLNLRLNTFTKTEFGALVEQFHSFKEQLLHSVEGQSSGTFEWVDGMLVQALQCGDWLLMDNVNFCSSSVLDRLNALLEPGGVLTMTERGVTDGSMLTITPHPNFRLFLSMDP-SHGE----ISRAMRNRGIELYISGESE 2234
BLAST of Midasin vs. Ensembl Xenopus
Match: MDN1 (midasin AAA ATPase 1 [Source:NCBI gene;Acc:100486293]) HSP 1 Score: 337.806 bits (865), Expect = 1.140e-113 Identity = 178/375 (47.47%), Postives = 243/375 (64.80%), Query Frame = 2
Query: 89 VVTIRDLLSWVNFINISIGNKTSL-----------YQSIFHGAYMVIIDSIND-KLNKSFYEE-----LLIKKLDYLVKSSFPDIVECLDF--QSSRFELSVKENKVGIDPFYIELGSHSDWXXXXXXXXXXKNFQYSFNSKTTGSNVYRLFRALQVSKKPLLLEGSPGVGKTSLVTQLAKLVGFKVVRINLSEQTDANDLFGCFLPNENGKAGELSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPEINQKFMVEPGKTRLFACQNPVSEGSGRKGLPKSFVNRFTQVYMSSLSDSDYRNILNCLYPAIARETIGFIVEFVQNLREHVVVMKKFGTKGSPWEFNLRDLCRWCDLMVKHQ 1156
++++RD+LSWVNF+N+ +G+K ++ + H A +V ID + +L+ + + L +KL ++ S D+ L Q+++ ++ +E+ GI PFYI +G + +K Y+ N++TT N RL RALQ++K P++LEGSPGVGKTSLVT LAK G +VRINLSEQTD DLFG LP E GK GE +WRDGPLL ALKAG WI+LDE+NLASQSVLE LNAC DHR E++IPE+ F V+ +T++F CQNP +G GRKGLP+SF+NRFTQVY+ LS +D I N L+P++ T+ +V F + V+V KK+G KG PWEFNLRDL RWC LM++ Q
Sbjct: 1534 ILSVRDILSWVNFMNVMVGDKDAVNSNAPGADLDAVTAFVHAACLVYIDGLGSGELSSATFARQQCMRFLREKLSEIL-SLTEDVHSELKLYDQATQRKICWREDCFGIHPFYIPIGPY---------FQENKITDYALNAETTSMNAQRLLRALQLNK-PIILEGSPGVGKTSLVTALAKASGNCLVRINLSEQTDVTDLFGTDLPVEGGKGGEFAWRDGPLLSALKAGHWIVLDELNLASQSVLEGLNACFDHRAEVYIPELGMNFQVQHERTKIFGCQNPFHQGGGRKGLPRSFLNRFTQVYVDQLSFADMEFIANSLFPSLGTSTVSKMVAFNNKIDHQVMVEKKWGQKGGPWEFNLRDLFRWCQLMLEDQ 1897 HSP 2 Score: 97.0561 bits (240), Expect = 1.140e-113 Identity = 57/132 (43.18%), Postives = 82/132 (62.12%), Query Frame = 1
Query: 1159 RTKEDQDACGKVYNELADKSRFARFIPSGLDFYITDQVLKIGQTQLSVNIANNTDNSKQFNKHL-LLAGQLAILESMMLTVDMGWLTVLVGPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGSFEQ 1551
RTK D++ VY E+ + ++ + FYIT Q +++G + L + D+ F+ HL LL L LE +M V MGW+ +LVGPA GKT++V+ L+ +TG+ LK M+MNSAMDTT+LLG FEQ
Sbjct: 1917 RTKPDREKIISVYKEVYGQD-LTPYLGTRY-FYITPQNVQLGYSVL----PRSCDSPLSFSGHLSLLHHSLQSLEPVMKCVQMGWMVILVGPAAVGKTSLVQTLALLTGHKLKIMAMNSAMDTTELLGGFEQ 2042 HSP 3 Score: 136.732 bits (343), Expect = 6.797e-31 Identity = 89/211 (42.18%), Postives = 119/211 (56.40%), Query Frame = 1
Query: 1423 VGPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDT---------TDLLGSFEQQ-------KEGGKFKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGLVYGDRIEEIKPHKNFRLFLILDPDCGGEISKAMRNRGLEICIIPENRELTLTDNDLLAILITIYKLSVKNADSLLRFHKTMILAQSETPQIKIGN 2007
VG AG+G TT LL+ + G ++ +N ++T + SF++Q + G F+WVD LVQAL+ GDWL++D VNFCS+SVLDRLN LLEP G L +TERG+ G + I PH NFRLFL +DP GEIS+AMRNRG+E+ I E+ + L DL I+ L V D +I SET + G+
Sbjct: 2097 VGEAGSGITT--DLLNKLEGVLVQIQRLNLRLNTFTKTEFGALVEQFHSFKEQLLHSVEGQSSGTFEWVDGMLVQALQCGDWLLMDNVNFCSSSVLDRLNALLEPGGVLTMTERGVTDGSML-TITPHPNFRLFLSMDPS-HGEISRAMRNRGIELYISGESEGMLL---DLFDTKRLIHNLGVVGDDVC----NALIAVHSETKEAVTGS 2296 HSP 4 Score: 134.806 bits (338), Expect = 2.710e-30 Identity = 110/384 (28.65%), Postives = 171/384 (44.53%), Query Frame = 2
Query: 23 VAGIIVRFLLIVQKMS--------HEINFSVVTIRDLLSWVNFINISIGNKTSLYQSIFHGAYMVIIDSINDKLNKSFYEELLIK-KLDYLVKSSFPDIVECLDFQSSRFELSVKENKVGIDPFYIELGSHSDWXXXXXXXXXXKNFQYSFNSKTTGSNVYRLFRALQVSKKPLLLEGSPGVGKTSLVTQLAKLVGFKVVRINLSEQTDANDLFGCFLPNENGKAGELSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPEINQKFMVEPGKTRLFACQNPVSEGSGRKGLPKSFVNRFTQVYMSSLSDSDYRNILN---CLYPAIARETIGFIVEFVQNLREHVVVMKKFGTKGSPWEFNLRDLCRWCD 1138
V GII +L I +K S H ++S+ T+ L + + N + SLY+ G ++ +D + + +++ + +L+ P+ Q + + V + ID YI YS N N+ L R + P+L++G VGKTSL+ LA G VRIN E TD + GC+ + +GK L +++G L+ A++ G W++LDE+NLA VLE LN LD E+FI E + P + LFA QNP GRK L ++F NRF +++ L ++ IL+ CL P+ + + ++E R V K G LRDL RW +
Sbjct: 882 VQGIISFYLAIRKKSSAELVDGTGHRPHYSLRTLCRALRYAS-ANPCNSVQRSLYEGFCLG-FLTQLDRASHSVVLGLIRRYIVQGNIKHLLSQPLPEPRGSRFVQIQGYWIPVGDKDPAIDESYI--------------------LTYSVNL-----NLRDLVRVVSAGTHPVLIQGETSVGKTSLIRWLACASGNHCVRINNHEHTDIQEYIGCYTSDVSGK---LIFKEGVLIDAMRKGYWVILDELNLAPTDVLEALNRLLDDNRELFITETQEVVKAHP-RFMLFATQNPPGLYGGRKVLSRAFRNRFVELHFDELPSAELETILHKRCCLPPSYCNKLVAVMLELQSYRRGSSVFAGKHGF------ITLRDLFRWAE 1228 HSP 5 Score: 92.4337 bits (228), Expect = 1.676e-17 Identity = 75/258 (29.07%), Postives = 131/258 (50.78%), Query Frame = 2
Query: 455 KTTGSNVYRLFRALQVS-KKPLLLEGSPGVGKTSLVTQLAKLVGFK----VVRINLSEQTDANDLFGCFLPNENGKAGELSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPEINQKFMVEPGKTRLFACQNPVSEGSGRKGLPK----SFVNR-FTQVYMSSLSDSDYRNILNCLYPAIARETIGFIVEFVQNLREHVVVMKKFGTKGS-----PWEFN------------LRDLCRWCDLMV 1147
++T N++ L AL VS + P+LLEG G GKT+LV LA + G ++++ L +QTD+ L G + + GE W+ G L QA+ G WILL++++ A V+ L L++ GE+ +P V PG +LFA + +S SG P+ S +++ +T++++ ++S + + ++ LYP + T + ++Q + + M T+ P E N LRDL +WC+ ++
Sbjct: 290 ESTCKNLHSL--ALAVSYQNPVLLEGPIGCGKTALVEYLATVTGRHQPPDILKVQLGDQTDSKMLLGMYRCTD--IPGEFVWQPGTLTQAVANGHWILLEDIDYAPLDVISVLLPVLEN-GELLMPGRGDCIKVAPG-FQLFATRRLLSSSSGVWYRPQNTHASLLDKHWTRIHLGNMSREELKEVILKLYPNLELVTDRLLDIYIQLTGDKHLAMSDLETEAEALLQKPLEQNVPRPNLEGRGLSLRDLLKWCNRII 541 HSP 6 Score: 83.9593 bits (206), Expect = 5.530e-15 Identity = 86/328 (26.22%), Postives = 146/328 (44.51%), Query Frame = 1
Query: 1363 QLAILESMMLTVDMGWLTVLVGPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGSFEQQKEGGK---FKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGLVYGDRIEEIK---PHKNFRLFLILDPDCGG-----EISKAMRNRGLEI-C-----------IIPENRELTLTDNDLLAILITIY-------------KLSVKNADSLLRFHKTMI----LAQSETPQIKIGNINSLIQAAKLIK-DGYSLDQSLRSVYGDIQMLFNVRDDVNRFIN---DCNEEVIDYQEIEQDDFSTCSSLFNI 2214
+LA+L L G +LVG G GKTTI +L + + L ++ + M+T+D LG ++ K F+W D LV A+++ + ++D ++ SVL+RLN +LE +L+L E+G D EE++ K FR+ ++P GG E+S A+RNR EI C II N L+ +A L+ + LSV++ S + F M+ S P + + + + AA L+ DG + + + Q + +R+ ++ ++ D + E+ Y + Q F I
Sbjct: 1319 RLAVLVGRAL--QFGEPVLLVGDTGCGKTTICQLFAALASQTLHCVNCHQHMETSDFLGGLRPIRQRSKDKLFEWHDGPLVLAIKEDGFFLMDEISLADDSVLERLNSVLEVEKTLVLAEKG-SGEDETEEVEILTAGKQFRILATMNP--GGDFGKKELSPALRNRFTEIWCPQSNDRHDLAEIIKHNLHTGLSLGADIADLMMDFIEWLTNQDFGRRCILSVRDILSWVNFMNVMVGDKDAVNSNAPGADLDAVTAFVHAACLVYIDGLGSGELSSATFARQQCMRFLREKLSEILSLTEDVHSELKLYDQATQRKICWREDCFGI 1641 HSP 7 Score: 79.7221 bits (195), Expect = 1.270e-13 Identity = 52/157 (33.12%), Postives = 80/157 (50.96%), Query Frame = 2
Query: 689 DGPLLQALKAGDWILLDEMNLASQSVLECLNACLD--HRGEIFIPEINQKFMVEPGKTRLFACQNPVSEGSGRKGLPKSFVNRFTQVYMSSLSDS--------DYRNILNCLYPAIARETIGFIVEFVQNLREHVVVMKKFGTKGSPWEFNLRDLCR 1129
+G L QA+K G+WILLDE+NLA+ LECL L+ + + + + +V RLFAC NP ++ G++ LP NRFT++Y+ L + DY LN +++ + I+ F +R+ GT P ++LR LCR
Sbjct: 768 EGTLAQAVKKGEWILLDEINLAAAETLECLCGLLEGISGSLVLLDRGDTEPLVRHPDFRLFACMNPATD-VGKRNLPPGIRNRFTELYVEELENEGDLQILIMDYLRGLN-----VSKTVVQGIISFYLAIRKKSSAELVDGTGHRP-HYSLRTLCR 917 HSP 8 Score: 76.6406 bits (187), Expect = 8.800e-13 Identity = 53/168 (31.55%), Postives = 84/168 (50.00%), Query Frame = 2
Query: 488 RALQVSKKPLLLEGSPGVGKTSLVTQLAKLVGFKVVRINLSEQTDANDLFGCFLP-NENGKAGELSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIP-------EINQKFMVEPGKT-RLFACQNPVSEGSGRKGLPKSFVNRFTQVYMSSLSD 964
RALQ + P+LL G G GKT++ A L + +N + + +D G P + K W DGPL+ A+K + L+DE++LA SVLE LN+ L+ + + E + ++ GK R+ A NP + G+K L + NRFT+++ +D
Sbjct: 1326 RALQFGE-PVLLVGDTGCGKTTICQLFAALASQTLHCVNCHQHMETSDFLGGLRPIRQRSKDKLFEWHDGPLVLAIKEDGFFLMDEISLADDSVLERLNSVLEVEKTLVLAEKGSGEDETEEVEILTAGKQFRILATMNPGGD-FGKKELSPALRNRFTEIWCPQSND 1491 HSP 9 Score: 75.8702 bits (185), Expect = 1.794e-12 Identity = 51/161 (31.68%), Postives = 82/161 (50.93%), Query Frame = 1
Query: 1375 LESMMLTVDMGWLTVLV-GPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGSFEQQKEGGKFKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGLVYGDRIEEIKPHKNFRLFLILDPD--CGGE--ISKAMRNRGLEI 1842
L ++ V G VL+ G GKT++++ L+ +GNH ++ + D + +G + GK + + L+ A+R+G W+ILD +N VL+ LN LL+ N L +TE E +K H F LF +P GG +S+A RNR +E+
Sbjct: 1014 LRDLVRVVSAGTHPVLIQGETSVGKTSLIRWLACASGNHCVRINNHEHTDIQEYIGCYTSDV-SGKLIFKEGVLIDAMRKGYWVILDELNLAPTDVLEALNRLLDDNRELFITETQ-------EVVKAHPRFMLFATQNPPGLYGGRKVLSRAFRNRFVEL 1166 HSP 10 Score: 75.0998 bits (183), Expect = 2.728e-12 Identity = 39/114 (34.21%), Postives = 64/114 (56.14%), Query Frame = 1
Query: 1417 VLVGPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGS--FEQQKEGGKFKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGLVYGDRIEEIK 1752
+L G G GKT++V L+ +GN L ++++ D TDL G+ + +GG+F W D L+ AL+ G W++LD +N S SVL+ LN + + + E G+ + + E K
Sbjct: 1685 ILEGSPGVGKTSLVTALAKASGNCLVRINLSEQTDVTDLFGTDLPVEGGKGGEFAWRDGPLLSALKAGHWIVLDELNLASQSVLEGLNACFDHRAEVYIPELGMNFQVQHERTK 1798 HSP 11 Score: 72.4034 bits (176), Expect = 2.179e-11 Identity = 100/420 (23.81%), Postives = 176/420 (41.90%), Query Frame = 1
Query: 5371 PHDIYQDPIDVDNLLQAQKILPNANVSVTKLLGIYPEHTTXXXXXXXXXXXXXXSG-DYSLIVFINNVERLI-----------HEIQQWEVVAARHVSLIQHVDELNGLLVSWRHQELTM-----WENTISTANWQLERAVSREFFNHYNLIVQESNKIQYDNDFESINLILEPILNSLL-NSTVGNFEYRLKTMKLSIESLISNREIDYRFKDRLKNILLNHYDFYRLYLGNVQEHLSSEYKRIGKELRDFIKTKRWGKTP--PKITDVLKCHKIVFKYKRQWSGIINQPCTKFFV-----------------LTPINALKQVNLIDIGNCIESDIDFSKSKLSDWCATMYEYSREMWETMENFQKETKELEDISVVQSKSEDDKEALEKKWRMNVKNLQQRKRNCVSQFVKELREMGL 6519
P+D YQ P ++ + Q QKIL N ++ KLL +PEH L+ + KKI ++ G L +N L+ H Q WE A R +SL +H+DE+ L + + W + A + + + +F+ Y L+ E + + E + L L + + ST+G F+ RL T+ L + + KD L ++L N Y +Y+ + VQ ++ + KEL++F+K RW V K H+ +FKY +++ ++ +PC+ IN L+Q+ L I+ + + A M + E EL++++V D A ++K + K++ +K+ +S+ K L + GL
Sbjct: 3458 PYDFYQHP-NIPQVQQCQKILQNFTSAINKLLEEWPEHPALVQVSKKIFRVMIYLGFQRKLYSNVNKKISLLFSSTIYICLFPHRGQDWEENANRGLSLRKHLDEITHTLFQKCLNNINLSVFSCWSLGLDNALARHVQKTTSHWFSVYQLV--EKHLQEEHEQEELLLQSLTKTLQAFIEGSTLGEFQSRL-TLLLVFHCHVLLIPQSPK-KDSLCSVLWNLYHYYKQFASTVQARITELRNPLEKELKEFVKISRWNDVSFWSVKQSVDKTHRTLFKYMKKFETVLCEPCSPALTESAQEERLEYQEDQRESAAKINQLQQLCLTLTAAVIKEEQTLR------YIAMM-----SFFYVAEGIIASVTELQNLTV-------DHNADKEKQKSQAKHILMQKQRALSELFKMLTKTGL 3854 HSP 12 Score: 69.3218 bits (168), Expect = 1.588e-10 Identity = 47/155 (30.32%), Postives = 77/155 (49.68%), Query Frame = 1
Query: 1324 NSKQFNKHLLLAGQLAILESMMLTVDMGWLTVLVGPAGTGKTTIVKLLSDITGNH----LKSMSMNSAMDTTDLLGSFEQQKEGGKFKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGLVYGDRIEEIKPHKNFRLF 1776
+S+ ++ +L+ L S+ L V +L GP G GKT +V+ L+ +TG H + + + D+ LLG + G+F W TL QA+ G W++L+ +++ V+ L +LE NG LL+ RG + IK F+LF
Sbjct: 279 HSESISQLVLVESTCKNLHSLALAVSYQNPVLLEGPIGCGKTALVEYLATVTGRHQPPDILKVQLGDQTDSKMLLGMYRCTDIPGEFVWQPGTLTQAVANGHWILLEDIDYAPLDVISVLLPVLE-NGELLMPGRG-------DCIKVAPGFQLF 425 HSP 13 Score: 63.1586 bits (152), Expect = 1.198e-8 Identity = 37/97 (38.14%), Postives = 59/97 (60.82%), Query Frame = 1
Query: 1570 FKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEP-NGSLLLTERGLVYGDRIEEIKPHKNFRLFLILDP--DCGGE-ISKAMRNRGLEICI 1848
F +V+ TL QA+++G+W++LD +N +A L+ L GLLE +GSL+L +RG E + H +FRLF ++P D G + +RNR E+ +
Sbjct: 764 FAFVEGTLAQAVKKGEWILLDEINLAAAETLECLCGLLEGISGSLVLLDRG-----DTEPLVRHPDFRLFACMNPATDVGKRNLPPGIRNRFTELYV 855 HSP 14 Score: 59.3066 bits (142), Expect = 1.595e-7 Identity = 48/163 (29.45%), Postives = 74/163 (45.40%), Query Frame = 2
Query: 527 GSPGVGKTSLVTQLAKLVGFKVVRINLSEQTDANDLFGCFLPN------------ENGKAGELSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPEI----NQKFMVEPGKT-RLFACQNPVSEGSGRKGLPKSFVNRFTQVYMSSLSD 964
G G G T+ + + V ++ R+NL T FG + E +G W DG L+QAL+ GDW+L+D +N S SVL+ LNA L+ G + + E + P RLF +P S G + ++ NR ++Y+S S+
Sbjct: 2098 GEAGSGITTDLLNKLEGVLVQIQRLNLRLNTFTKTEFGALVEQFHSFKEQLLHSVEGQSSGTFEWVDGMLVQALQCGDWLLMDNVNFCSSSVLDRLNALLEPGGVLTMTERGVTDGSMLTITPHPNFRLFLSMDP-SHGE----ISRAMRNRGIELYISGESE 2255
BLAST of Midasin vs. Ensembl Xenopus
Match: cybc1 (cytochrome b-245 chaperone 1 [Source:Xenbase;Acc:XB-GENE-977743]) HSP 1 Score: 56.9954 bits (136), Expect = 8.084e-7 Identity = 47/220 (21.36%), Postives = 90/220 (40.91%), Query Frame = 2
Query: 509 KPLLLEGSPGVGKTSLVTQLAKLVGFKVVRINLSEQTDANDLFGC--FLPNENGKAGELSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPEINQKFMVEPGKTRLFACQNPVSEGSGRKGLPKSFVNRFTQVYMSSLSDSDYRNILNCLYPAIARETIGFIVEFVQNLREHVVVMKKFGTKGSPWEFNLRDLCRWCDLMVKHQHE 1162
K + L G G GK+ + + A ++G+++ + L + A DL LPN G+ +WR PL+ A G ++LD ++ + L L + F + P + + PV G+ ++ L + F + SL+ + I+ + P +A+E + ++ LR+ K + + R L R C + ++ E
Sbjct: 434 KDMCLIGGKGCGKSVIAKEFAGMLGYEIEPVMLYQDMTARDLLQQRFTLPN-----GDTAWRQSPLVTAAIEGKLVILDGIHRVNPGTLSILQRSI--------------FPIHPSFRIIALAEPPVLGGNSQQWLGPELLTMFLFHEIKSLTKKEEIQIITGMVPGVAKEAVEQLLTVTHKLRD----TKDPTAQSLASSLSTRQLLRICRRLSQYPEE 630
BLAST of Midasin vs. Ensembl Mouse
Match: Mdn1 (midasin AAA ATPase 1 [Source:MGI Symbol;Acc:MGI:1926159]) HSP 1 Score: 318.931 bits (816), Expect = 5.531e-105 Identity = 182/402 (45.27%), Postives = 238/402 (59.20%), Query Frame = 2
Query: 11 KSNSVAGIIVRFL--LIVQKMSHEINFSVVTIRDLLSWVNFIN-------ISIGNKTSLYQSIFHGAYMVIIDSINDKLNKSFY-------EE---LLIKKLDYLVKSSFPDIVECLDFQSSRF-ELSVKENKVGIDPFYIELGSHSDWXXXXXXXXXXKNFQYSFNSKTTGSNVYRLFRALQVSKKPLLLEGSPGVGKTSLVTQLAKLVGFKVVRINLSEQTDANDLFGCFLPNENGKAGELSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPEINQKFMVEPGKTRLFACQNPVSEGSGRKGLPKSFVNRFTQVYMSSLSDSDYRNILNCLYPAIARETIGFIVEFVQNLREHVVVMKKFGTKGSPWEFNLRDLCRWCDLMVKHQ 1156
K VA +++ F+ L Q+ + VV+IRD+LSWVNF+N + S S H A +V ID I + S + EE LIKKL +V+ + E + R E + ++ G+ PF+I G D Y+ + TT N RL RA +++K P+LLEGSPGVGKTSLV LAK G +VRINLSEQTD DLFG LP E G+ GE +W DGPLL ALKAG W++LDE+NLASQS+LE LNAC DHRGEI++PE+ F V+ KTR+F CQNP +G GRKGLPKSF+NRFTQV++ L+ D I + L+P I ++ + +V F ++ V V KK+G KG PWEFNLRDL RWC L++ Q
Sbjct: 1569 KGAGVAEVMLAFITWLTRQEFGRKC---VVSIRDILSWVNFMNSMAEDAAVKRPEAISTVTSFVHAACLVYIDGIGSGVTSSGFGTALLAREECLKFLIKKLSKVVRLTQRQKDELKIYDRLRHKEFTSIDDFWGVHPFFIPRGPVLD---------GHNTGDYALTAGTTAMNAQRLLRAAKLNK-PILLEGSPGVGKTSLVAALAKASGNTLVRINLSEQTDITDLFGADLPVEGGRGGEFAWCDGPLLAALKAGHWVVLDELNLASQSILEGLNACFDHRGEIYVPELGMSFQVQHEKTRIFGCQNPFRQGGGRKGLPKSFLNRFTQVFVDPLTVVDMEFIASTLFPTIDKDIVKKMVAFNNHIDHEVTVEKKWGQKGGPWEFNLRDLFRWCQLILVDQ 1957 HSP 2 Score: 86.2705 bits (212), Expect = 5.531e-105 Identity = 50/131 (38.17%), Postives = 76/131 (58.02%), Query Frame = 1
Query: 1159 RTKEDQDACGKVYNELADKSRFARFIPSGLDFYITDQVLKIGQTQLSVNIANNTDNSKQFNKHLLLAGQLAILESMMLTVDMGWLTVLVGPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGSFEQ 1551
RT+ED++ V+ ++ + F+IT ++IG + LS + + + + LL LE +M V M W+ +LVGPA GKT++V+LL+ +TG+ LK M+MNSAMDTT+LLG FEQ
Sbjct: 1977 RTREDKEKVVAVFKDVFASNSMPYI--GTRQFHITPYDIQIGYSVLSRSSYVPHPSRRPLS---LLHQSFQSLEPIMKCVQMNWMVILVGPASVGKTSLVQLLAHLTGHTLKIMAMNSAMDTTELLGGFEQ 2102 HSP 3 Score: 227.254 bits (578), Expect = 2.171e-58 Identity = 126/283 (44.52%), Postives = 184/283 (65.02%), Query Frame = 1
Query: 9145 WSQYESKTEYLSRQLCEMLRIVLEPTKANKLGGAYRTGKRLNMKKVIPYIASGFRKDKIWLRRNKPSQREYQVIIGVDNSKSMAYNVNHQLFFESLSIVLNGLTFLEVGQVGLCKFGAKAEVVLPLYEKFSSEIGDRLIETFDFSDKCSKFIPILEQSIKLFEENQNFLSSSAVRXXXXXXXXXXXXXXXXXXXXPKFISLIREAHNKGIFVVVILLDHADSKDSILNLKMVIDDG----CDFVRFLDVFPLQNYTVLQDVTKLPTLLTDVLRQWFELSSSAD 9981
W Y T LS+QLCE LR++LEPT+A KL G YRTGKRLNM+K+IPYIAS FRKD+IWLRR KPS+R+YQ+ + +D+S SM N QL FESL+++ N LT LEVGQ+ +C FG +++ P +E+F+ G ++ F + +K LE K+F Q LS + +T+QLLL++SDG + ++ ++ A N IFV+ ++LD+ +S+DSIL++K+ I G + +++ FP Y +L+DV LP L+D LRQWFEL +++D
Sbjct: 5306 WQNYLVLTASLSQQLCEQLRLLLEPTQAAKLRGDYRTGKRLNMRKIIPYIASQFRKDRIWLRRTKPSKRQYQICLAIDDSSSMVDNHTKQLAFESLAVIGNALTLLEVGQIAVCSFGESVKLLHPFHEQFNDSSGTHILRLCTFQQRKTKIAQFLETVAKMFAAAQK-LSQNVSPETAQLLLIVSDGRGLFLEGKDRVLAAVQAAQNANIFVIFVVLDNPNSRDSILDIKVPIFKGPGEMPEIRSYMEEFPFPFYIILRDVNALPETLSDALRQWFELVTASD 5587 HSP 4 Score: 127.487 bits (319), Expect = 3.761e-28 Identity = 75/174 (43.10%), Postives = 106/174 (60.92%), Query Frame = 1
Query: 1555 KEGGKFKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGLVYGDRIEEIKPHKNFRLFLILDPDCGGEISKAMRNRGLEICIIPENRELTLTDN-DLLAILITIYKLSVKNADSLLRFHKTMILAQSETPQIKIGNI-NSLIQAAKLIKDGYSLDQSL 2070
+ G F+WVDS LVQAL+ GDWL++D VNFC+ SVLDRLN LLEP G L + ERG+V G + P+ NFRLFL +DP GEIS+AMRNRGLEI I E + ++ DN DL +L ++ + + LL H + +P + + ++ I + ++ G SL ++
Sbjct: 2212 RSHGSFEWVDSMLVQALKSGDWLLMDNVNFCNPSVLDRLNALLEPGGVLTINERGMVDGSTC-TVTPNPNFRLFLSMDP-IHGEISRAMRNRGLEIYISGEE-DGSIQDNLDLKVLLHSLGLVGDSVCNILLALHTEIQSLVRGSPASSVSTLSHTAILIVQYLQRGLSLHKAF 2382 HSP 5 Score: 125.176 bits (313), Expect = 1.657e-27 Identity = 79/226 (34.96%), Postives = 114/226 (50.44%), Query Frame = 2
Query: 470 NVYRLFRALQVSKKPLLLEGSPGVGKTSLVTQLAKLVGFKVVRINLSEQTDANDLFGCFLPNENGKAGELSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPEINQKFMVEPGKTRLFACQNPVSEGSGRKGLPKSFVNRFTQVYMSSLSDSDYRNILN---CLYPAIARETIGFIVEFVQNLREHVVVMKKFGTKGSPWEFNLRDLCRWCD 1138
N+ + R + P+L++G VGKTSL+ LA G VRIN E TD + GC+ + +GK L + +G L+ A++ G WI+LDE+NLA VLE LN LD E+ I E + P + LFA QNP GRK L ++F NRF +++ L S+ IL+ L P+ + + +++ R V K G LRDL RW +
Sbjct: 1061 NLRDIARVVSAGTYPVLIQGETSVGKTSLIRWLAAASGNHCVRINNHEHTDIQEYIGCYTSDTSGK---LVFNEGVLIDAMRKGYWIVLDELNLAPTDVLEALNRLLDDNRELLITETQEVVRAHP-RFMLFATQNPPGLYGGRKVLSRAFRNRFVELHFDELPSSELETILHKRCSLPPSYCSKLVKVMLDLQSYRRRSSVFAGKQGF------IALRDLFRWAE 1276 HSP 6 Score: 115.931 bits (289), Expect = 1.031e-24 Identity = 87/347 (25.07%), Postives = 172/347 (49.57%), Query Frame = 1
Query: 5191 EEKQDTTVFRDCYRFAAALISS--DNQGIPSDDCKLFAANLSALNWEYETLSPGVPQNTIGRP---HDIYQDPIDVDNLLQAQKILPNANVSVTKLLGIYPEHTTXXXXXXXXXXXXXXSGDYSLIVFINNVERLIHEIQQWEVVAARHVSLIQHVDELNGLLVSWRHQELTMWENTISTANWQLERAVSREFFNHYNLI---VQESNKIQYDNDFESINLILEPILNSLLNSTVGNFEYRLKTMKLSIESLISNREIDYRFKDRLKNILLNHYDFYRLYLGNVQEHLSSEYKRIGKELRDFIKTKRWGKTP--PKITDVLKCHKIVFKYKRQWSGIINQPCTKFFV 6201
E K ++F CY+ A+L++ G+ +D +L + L A TL + + RP +D YQ P +V Q Q +L + +V++LL +PEH L + ++ I + F+N +E L+ + Q WE A+R +SL +H+D ++ +++ WR EL W ++ + ++ +F+ Y ++ +QE + Q D+ ++ L++ + + S++G F RL+ + + ++ +++ KD L ++L N Y FY+ +L V+ + I KEL++F+K +W V K H+ +FK+ +++ ++++PC V
Sbjct: 3639 EAKHYLSLFLSCYQTGASLVTHFYPLMGVELND-QLLGSQLLACTLSSNTLCGEATSDLMMRPDGPYDFYQHP-NVAEARQCQPVLQGFSEAVSQLLQDWPEHPVLQQLLVVMDRIRGFPLSSPISKFLNGLEILLAKAQDWEENASRVLSLRKHLDLVSQMIIRWRKLELNCWSMSLDNTMIRHTEKSTKHWFSIYQMLEKHMQERTEEQEDDKQMTLMLLVSTLQAFIEGSSLGEFHVRLQMLLVFHCHVLLMPQVEG--KDSLCSVLWNLYHFYKQFLDPVKAKIMELRSPIEKELKEFVKISKWNDVSFWSIKQSVEKTHRTLFKFMKKFEAVLSEPCQSCLV 3981 HSP 7 Score: 86.2705 bits (212), Expect = 1.082e-15 Identity = 54/152 (35.53%), Postives = 81/152 (53.29%), Query Frame = 2
Query: 689 DGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGE--IFIPEINQKFMVEPGKTRLFACQNPVSEGSGRKGLPKSFVNRFTQVYMSSLSDSDYRNILNCLYPA---IARETIGFIVEFVQNLREHVVVMKKFGTKGSPWEFNLRDLCR 1129
+G L QA+K G+WILLDE+NLA+ LECL+ L+ + + + + +V RLFAC NP ++ G++ LP NRFT++Y+ L + IL Y +++ T+ IV F LR+ GT P ++LR LCR
Sbjct: 816 EGTLAQAIKKGEWILLDEINLAAPETLECLSGLLEGSSGSLVLLDRGDTEPLVRHPDFRLFACMNPATD-VGKRNLPPGIRNRFTELYVEELESKEDLQILIVDYLKGLNVSKNTVQGIVNFYTALRKESGTKLVDGTGHRP-HYSLRTLCR 965 HSP 8 Score: 84.3445 bits (207), Expect = 4.029e-15 Identity = 62/185 (33.51%), Postives = 97/185 (52.43%), Query Frame = 1
Query: 1363 QLAILESMMLTVDMGWLTVLVGPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGSFE--QQKEGGK-------FKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGLVYG--DRIEEIKPHKNFRLFLILDPDCGG-----EISKAMRNRGLEI-CIIPENRE 1866
+LA+L L + G +LVG G GKTT+ ++ S + L S++ + M+T+D LG +QK K F+W D LV A+++ + +LD ++ SVL+RLN +LE L+L E+G + +E + K+FR+ ++P GG E+S A+RNR EI C RE
Sbjct: 1367 RLAVLVGRAL--EFGEPVLLVGDTGCGKTTVCQMFSALANQKLYSVNCHLNMETSDFLGGLRPVRQKPNDKDELDTRLFEWHDGPLVLAMKEDSFFLLDEISLADDSVLERLNSVLEVEKCLVLAEKGSPESKDNEVELLTAGKHFRILATMNP--GGDFGKKELSPALRNRFTEIWCPQSTKRE 1547 HSP 9 Score: 82.0333 bits (201), Expect = 1.789e-14 Identity = 51/166 (30.72%), Postives = 87/166 (52.41%), Query Frame = 1
Query: 1417 VLVGPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGS--FEQQKEGGKFKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGLVYGDRIEEIKPHKNFRLFLILDP--DCGGE--ISKAMRNRGLEICIIPENRELTLTDNDLLA 1896
+L G G GKT++V L+ +GN L ++++ D TDL G+ + GG+F W D L+ AL+ G W++LD +N S S+L+ LN + G + + E G+ + + H+ R+F +P GG + K+ NR ++ + P LT+ D + +A
Sbjct: 1745 LLEGSPGVGKTSLVAALAKASGNTLVRINLSEQTDITDLFGADLPVEGGRGGEFAWCDGPLLAALKAGHWVVLDELNLASQSILEGLNACFDHRGEIYVPELGMSFQVQ------HEKTRIFGCQNPFRQGGGRKGLPKSFLNRFTQVFVDP----LTVVDMEFIA 1900 HSP 10 Score: 81.2629 bits (199), Expect = 3.555e-14 Identity = 72/249 (28.92%), Postives = 118/249 (47.39%), Query Frame = 2
Query: 500 VSKKPLLLEGSPGVGKTSLVTQLAKLVGF----KVVRINLSEQTDANDLFGCFLPNENGKAGELSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPEINQKFMVEPGKTRLFACQNPVSEGSGRKGLP----KSFVNRF-TQVYMSSLSDSDYRNILNCLYPAIARET---IGFIVEFV---------------QNLREHVVVMKKFGTKGSPWEFNLRDLCRWCDLMVKHQHEP 1165
S+ +LLEG G GKTSLV LA + G +++++ L +QTD+ L G + + GE W+ G L QA G WILL++++ A V+ L L+H GE+ IP V P +LFA + +S G G P + ++++ T++++ +L+ D +L YP++ T + +E Q + E ++ +G E +LRDL WC+ V H +P
Sbjct: 320 ASQNAVLLEGPIGSGKTSLVEHLAAVTGRTKPPQLLKVQLGDQTDSKMLLGMYRCTD--VPGEFVWQPGTLTQAATKGYWILLEDIDYAPLDVVSVLIPLLEH-GELLIPGHGDCLKVAP-TFQLFATRRLLSCG-GSWYRPMNSHATMLDKYWTKIHLHNLNKKDLNEVLQSKYPSLLAATDHLLDIYIELTGEKHCCPSVAYDKAPQEVSEAERENRRVVLEGR--ELSLRDLLNWCN-RVAHGFDP 560 HSP 11 Score: 76.6406 bits (187), Expect = 6.835e-13 Identity = 50/161 (31.06%), Postives = 82/161 (50.93%), Query Frame = 1
Query: 1375 LESMMLTVDMGWLTVLV-GPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGSFEQQKEGGKFKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGLVYGDRIEEIKPHKNFRLFLILDPD--CGGE--ISKAMRNRGLEI 1842
L + V G VL+ G GKT++++ L+ +GNH ++ + D + +G + GK + + L+ A+R+G W++LD +N VL+ LN LL+ N LL+TE E ++ H F LF +P GG +S+A RNR +E+
Sbjct: 1062 LRDIARVVSAGTYPVLIQGETSVGKTSLIRWLAAASGNHCVRINNHEHTDIQEYIGCYTSDT-SGKLVFNEGVLIDAMRKGYWIVLDELNLAPTDVLEALNRLLDDNRELLITETQ-------EVVRAHPRFMLFATQNPPGLYGGRKVLSRAFRNRFVEL 1214 HSP 12 Score: 74.7146 bits (182), Expect = 3.245e-12 Identity = 72/242 (29.75%), Postives = 111/242 (45.87%), Query Frame = 2
Query: 488 RALQVSKKPLLLEGSPGVGKTSLVTQLAKLVGFKVVRINLSEQTDANDLFGCFLP--NENGKAGEL-----SWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPEI-------NQKFMVEPGKT-RLFACQNPVSEGSGRKGLPKSFVNRFTQVYM-SSLSDSDYRNILNC-LYPAIARETIGFIVEFVQNLREHVVVMKKFGTKGSPWEF------NLRDLCRWCDLM 1144
RAL+ + P+LL G G GKT++ + L K+ +N + +D G P + EL W DGPL+ A+K + LLDE++LA SVLE LN+ L+ + + E N+ ++ GK R+ A NP + G+K L + NRFT+++ S D I+N L P ++ +G V VM F T + EF ++RD+ W + M
Sbjct: 1374 RALEFGE-PVLLVGDTGCGKTTVCQMFSALANQKLYSVNCHLNMETSDFLGGLRPVRQKPNDKDELDTRLFEWHDGPLVLAMKEDSFFLLDEISLADDSVLERLNSVLEVEKCLVLAEKGSPESKDNEVELLTAGKHFRILATMNPGGD-FGKKELSPALRNRFTEIWCPQSTKREDLIQIINHNLRPGLSLAGVGHKGAGVAE------VMLAFITWLTRQEFGRKCVVSIRDILSWVNFM 1607 HSP 13 Score: 73.9442 bits (180), Expect = 4.499e-12 Identity = 58/179 (32.40%), Postives = 93/179 (51.96%), Query Frame = 1
Query: 7435 SLDTQKIL----DLFTKIKSFFCDLNMNQVNKFRNLINEMV--LPKLRSLEKVEKEFLGLLLNYHRVLISATDALLTVTTKLMKEGFCIPNDSAWNNEAENSNPSEKMNDFEMAGMDGGVGTNDVTDEIESESQIE-----GNNKDKKDETENQPDIPDEDDGIEMTDDFNGKMHDPEI 7938
+L QKI+ +L ++KS D N + F +V +P L + FL + L HR L T+L ++GFC+P + ++ E + + +D+E G+ G G DV+D IE+E Q+E G KD++D +++PD ED+ IEM++DF+GKMHD E+
Sbjct: 4564 TLHVQKIISSVSELLERLKSCSEDGNTTKHKVFSQSCGLLVRLIPILCGFSDLVLFFLTVSLATHRSTAKLLSVLAQTFTELSQKGFCLPKELMEDSAGEGAT---QFHDYEGGGIGDGEGMKDVSDRIENEEQVEDTFQKGQEKDEED-LDSKPDTKGEDNAIEMSEDFDGKMHDGEL 4738 HSP 14 Score: 63.5438 bits (153), Expect = 7.443e-9 Identity = 36/97 (37.11%), Postives = 59/97 (60.82%), Query Frame = 1
Query: 1570 FKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLE-PNGSLLLTERGLVYGDRIEEIKPHKNFRLFLILDP--DCGGE-ISKAMRNRGLEICI 1848
F +V+ TL QA+++G+W++LD +N + L+ L+GLLE +GSL+L +RG E + H +FRLF ++P D G + +RNR E+ +
Sbjct: 812 FAFVEGTLAQAIKKGEWILLDEINLAAPETLECLSGLLEGSSGSLVLLDRG-----DTEPLVRHPDFRLFACMNPATDVGKRNLPPGIRNRFTELYV 903 HSP 15 Score: 61.2326 bits (147), Expect = 3.223e-8 Identity = 43/145 (29.66%), Postives = 70/145 (48.28%), Query Frame = 1
Query: 1414 TVLVGPAGTGKTTIVKLLSDITGN----HLKSMSMNSAMDTTDLLGSFEQQKEGGKFKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGLVYGDRIEEIKPHKNFRLFLILD-PDCGGEISKAMRNRG 1833
+L GP G+GKT++V+ L+ +TG L + + D+ LLG + G+F W TL QA +G W++L+ +++ V+ L LLE +G LL+ G + +K F+LF CGG + M +
Sbjct: 325 VLLEGPIGSGKTSLVEHLAAVTGRTKPPQLLKVQLGDQTDSKMLLGMYRCTDVPGEFVWQPGTLTQAATKGYWILLEDIDYAPLDVVSVLIPLLE-HGELLIPGHG-------DCLKVAPTFQLFATRRLLSCGGSWYRPMNSHA 461 HSP 16 Score: 57.7658 bits (138), Expect = 4.075e-7 Identity = 48/166 (28.92%), Postives = 84/166 (50.60%), Query Frame = 2
Query: 635 FGC-FLPNENGKA-GELSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPEINQKFMVEPGKT---------RLFACQNPVSEGSGRKGLPKSFVNRFTQVYMSSLSDSDYRNILNCLYPAIARETIGFIVEFVQNLREHVVVMKKFGTKGSP 1099
FG L + +G++ G W D L+QALK+GDW+L+D +N + SVL+ LNA L+ G + IN++ MV+ G T RLF +P+ + ++ NR ++Y+S D ++ L+ + ++G + + V N+ + + +GSP
Sbjct: 2201 FGVKLLQSASGRSHGSFEWVDSMLVQALKSGDWLLMDNVNFCNPSVLDRLNALLEPGG---VLTINERGMVD-GSTCTVTPNPNFRLFLSMDPI-----HGEISRAMRNRGLEIYISGEEDGSIQDNLDL---KVLLHSLGLVGDSVCNILLALHTEIQSLVRGSP 2354 HSP 17 Score: 56.6102 bits (135), Expect = 9.029e-7 Identity = 27/59 (45.76%), Postives = 38/59 (64.41%), Query Frame = 1
Query: 1372 ILESMMLTVDMGWLTVLVGPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGSFE 1548
+LE + + V G +LVG GTGKT+ V+ L+ TG HL+ ++MN DT DLLG F+
Sbjct: 656 LLEQLAVCVSQGEPVLLVGETGTGKTSAVQYLAYATGQHLRVVNMNQQSDTADLLGGFK 714
BLAST of Midasin vs. Ensembl Mouse
Match: Mdn1 (midasin AAA ATPase 1 [Source:MGI Symbol;Acc:MGI:1926159]) HSP 1 Score: 318.931 bits (816), Expect = 5.531e-105 Identity = 182/402 (45.27%), Postives = 238/402 (59.20%), Query Frame = 2
Query: 11 KSNSVAGIIVRFL--LIVQKMSHEINFSVVTIRDLLSWVNFIN-------ISIGNKTSLYQSIFHGAYMVIIDSINDKLNKSFY-------EE---LLIKKLDYLVKSSFPDIVECLDFQSSRF-ELSVKENKVGIDPFYIELGSHSDWXXXXXXXXXXKNFQYSFNSKTTGSNVYRLFRALQVSKKPLLLEGSPGVGKTSLVTQLAKLVGFKVVRINLSEQTDANDLFGCFLPNENGKAGELSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPEINQKFMVEPGKTRLFACQNPVSEGSGRKGLPKSFVNRFTQVYMSSLSDSDYRNILNCLYPAIARETIGFIVEFVQNLREHVVVMKKFGTKGSPWEFNLRDLCRWCDLMVKHQ 1156
K VA +++ F+ L Q+ + VV+IRD+LSWVNF+N + S S H A +V ID I + S + EE LIKKL +V+ + E + R E + ++ G+ PF+I G D Y+ + TT N RL RA +++K P+LLEGSPGVGKTSLV LAK G +VRINLSEQTD DLFG LP E G+ GE +W DGPLL ALKAG W++LDE+NLASQS+LE LNAC DHRGEI++PE+ F V+ KTR+F CQNP +G GRKGLPKSF+NRFTQV++ L+ D I + L+P I ++ + +V F ++ V V KK+G KG PWEFNLRDL RWC L++ Q
Sbjct: 1569 KGAGVAEVMLAFITWLTRQEFGRKC---VVSIRDILSWVNFMNSMAEDAAVKRPEAISTVTSFVHAACLVYIDGIGSGVTSSGFGTALLAREECLKFLIKKLSKVVRLTQRQKDELKIYDRLRHKEFTSIDDFWGVHPFFIPRGPVLD---------GHNTGDYALTAGTTAMNAQRLLRAAKLNK-PILLEGSPGVGKTSLVAALAKASGNTLVRINLSEQTDITDLFGADLPVEGGRGGEFAWCDGPLLAALKAGHWVVLDELNLASQSILEGLNACFDHRGEIYVPELGMSFQVQHEKTRIFGCQNPFRQGGGRKGLPKSFLNRFTQVFVDPLTVVDMEFIASTLFPTIDKDIVKKMVAFNNHIDHEVTVEKKWGQKGGPWEFNLRDLFRWCQLILVDQ 1957 HSP 2 Score: 86.2705 bits (212), Expect = 5.531e-105 Identity = 50/131 (38.17%), Postives = 76/131 (58.02%), Query Frame = 1
Query: 1159 RTKEDQDACGKVYNELADKSRFARFIPSGLDFYITDQVLKIGQTQLSVNIANNTDNSKQFNKHLLLAGQLAILESMMLTVDMGWLTVLVGPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGSFEQ 1551
RT+ED++ V+ ++ + F+IT ++IG + LS + + + + LL LE +M V M W+ +LVGPA GKT++V+LL+ +TG+ LK M+MNSAMDTT+LLG FEQ
Sbjct: 1977 RTREDKEKVVAVFKDVFASNSMPYI--GTRQFHITPYDIQIGYSVLSRSSYVPHPSRRPLS---LLHQSFQSLEPIMKCVQMNWMVILVGPASVGKTSLVQLLAHLTGHTLKIMAMNSAMDTTELLGGFEQ 2102 HSP 3 Score: 227.254 bits (578), Expect = 2.171e-58 Identity = 126/283 (44.52%), Postives = 184/283 (65.02%), Query Frame = 1
Query: 9145 WSQYESKTEYLSRQLCEMLRIVLEPTKANKLGGAYRTGKRLNMKKVIPYIASGFRKDKIWLRRNKPSQREYQVIIGVDNSKSMAYNVNHQLFFESLSIVLNGLTFLEVGQVGLCKFGAKAEVVLPLYEKFSSEIGDRLIETFDFSDKCSKFIPILEQSIKLFEENQNFLSSSAVRXXXXXXXXXXXXXXXXXXXXPKFISLIREAHNKGIFVVVILLDHADSKDSILNLKMVIDDG----CDFVRFLDVFPLQNYTVLQDVTKLPTLLTDVLRQWFELSSSAD 9981
W Y T LS+QLCE LR++LEPT+A KL G YRTGKRLNM+K+IPYIAS FRKD+IWLRR KPS+R+YQ+ + +D+S SM N QL FESL+++ N LT LEVGQ+ +C FG +++ P +E+F+ G ++ F + +K LE K+F Q LS + +T+QLLL++SDG + ++ ++ A N IFV+ ++LD+ +S+DSIL++K+ I G + +++ FP Y +L+DV LP L+D LRQWFEL +++D
Sbjct: 5299 WQNYLVLTASLSQQLCEQLRLLLEPTQAAKLRGDYRTGKRLNMRKIIPYIASQFRKDRIWLRRTKPSKRQYQICLAIDDSSSMVDNHTKQLAFESLAVIGNALTLLEVGQIAVCSFGESVKLLHPFHEQFNDSSGTHILRLCTFQQRKTKIAQFLETVAKMFAAAQK-LSQNVSPETAQLLLIVSDGRGLFLEGKDRVLAAVQAAQNANIFVIFVVLDNPNSRDSILDIKVPIFKGPGEMPEIRSYMEEFPFPFYIILRDVNALPETLSDALRQWFELVTASD 5580 HSP 4 Score: 127.487 bits (319), Expect = 3.824e-28 Identity = 75/174 (43.10%), Postives = 106/174 (60.92%), Query Frame = 1
Query: 1555 KEGGKFKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGLVYGDRIEEIKPHKNFRLFLILDPDCGGEISKAMRNRGLEICIIPENRELTLTDN-DLLAILITIYKLSVKNADSLLRFHKTMILAQSETPQIKIGNI-NSLIQAAKLIKDGYSLDQSL 2070
+ G F+WVDS LVQAL+ GDWL++D VNFC+ SVLDRLN LLEP G L + ERG+V G + P+ NFRLFL +DP GEIS+AMRNRGLEI I E + ++ DN DL +L ++ + + LL H + +P + + ++ I + ++ G SL ++
Sbjct: 2212 RSHGSFEWVDSMLVQALKSGDWLLMDNVNFCNPSVLDRLNALLEPGGVLTINERGMVDGSTC-TVTPNPNFRLFLSMDP-IHGEISRAMRNRGLEIYISGEE-DGSIQDNLDLKVLLHSLGLVGDSVCNILLALHTEIQSLVRGSPASSVSTLSHTAILIVQYLQRGLSLHKAF 2382 HSP 5 Score: 125.176 bits (313), Expect = 1.562e-27 Identity = 79/226 (34.96%), Postives = 114/226 (50.44%), Query Frame = 2
Query: 470 NVYRLFRALQVSKKPLLLEGSPGVGKTSLVTQLAKLVGFKVVRINLSEQTDANDLFGCFLPNENGKAGELSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPEINQKFMVEPGKTRLFACQNPVSEGSGRKGLPKSFVNRFTQVYMSSLSDSDYRNILN---CLYPAIARETIGFIVEFVQNLREHVVVMKKFGTKGSPWEFNLRDLCRWCD 1138
N+ + R + P+L++G VGKTSL+ LA G VRIN E TD + GC+ + +GK L + +G L+ A++ G WI+LDE+NLA VLE LN LD E+ I E + P + LFA QNP GRK L ++F NRF +++ L S+ IL+ L P+ + + +++ R V K G LRDL RW +
Sbjct: 1061 NLRDIARVVSAGTYPVLIQGETSVGKTSLIRWLAAASGNHCVRINNHEHTDIQEYIGCYTSDTSGK---LVFNEGVLIDAMRKGYWIVLDELNLAPTDVLEALNRLLDDNRELLITETQEVVRAHP-RFMLFATQNPPGLYGGRKVLSRAFRNRFVELHFDELPSSELETILHKRCSLPPSYCSKLVKVMLDLQSYRRRSSVFAGKQGF------IALRDLFRWAE 1276 HSP 6 Score: 115.931 bits (289), Expect = 1.039e-24 Identity = 87/347 (25.07%), Postives = 172/347 (49.57%), Query Frame = 1
Query: 5191 EEKQDTTVFRDCYRFAAALISS--DNQGIPSDDCKLFAANLSALNWEYETLSPGVPQNTIGRP---HDIYQDPIDVDNLLQAQKILPNANVSVTKLLGIYPEHTTXXXXXXXXXXXXXXSGDYSLIVFINNVERLIHEIQQWEVVAARHVSLIQHVDELNGLLVSWRHQELTMWENTISTANWQLERAVSREFFNHYNLI---VQESNKIQYDNDFESINLILEPILNSLLNSTVGNFEYRLKTMKLSIESLISNREIDYRFKDRLKNILLNHYDFYRLYLGNVQEHLSSEYKRIGKELRDFIKTKRWGKTP--PKITDVLKCHKIVFKYKRQWSGIINQPCTKFFV 6201
E K ++F CY+ A+L++ G+ +D +L + L A TL + + RP +D YQ P +V Q Q +L + +V++LL +PEH L + ++ I + F+N +E L+ + Q WE A+R +SL +H+D ++ +++ WR EL W ++ + ++ +F+ Y ++ +QE + Q D+ ++ L++ + + S++G F RL+ + + ++ +++ KD L ++L N Y FY+ +L V+ + I KEL++F+K +W V K H+ +FK+ +++ ++++PC V
Sbjct: 3639 EAKHYLSLFLSCYQTGASLVTHFYPLMGVELND-QLLGSQLLACTLSSNTLCGEATSDLMMRPDGPYDFYQHP-NVAEARQCQPVLQGFSEAVSQLLQDWPEHPVLQQLLVVMDRIRGFPLSSPISKFLNGLEILLAKAQDWEENASRVLSLRKHLDLVSQMIIRWRKLELNCWSMSLDNTMIRHTEKSTKHWFSIYQMLEKHMQERTEEQEDDKQMTLMLLVSTLQAFIEGSSLGEFHVRLQMLLVFHCHVLLMPQVEG--KDSLCSVLWNLYHFYKQFLDPVKAKIMELRSPIEKELKEFVKISKWNDVSFWSIKQSVEKTHRTLFKFMKKFEAVLSEPCQSCLV 3981 HSP 7 Score: 86.2705 bits (212), Expect = 1.029e-15 Identity = 54/152 (35.53%), Postives = 81/152 (53.29%), Query Frame = 2
Query: 689 DGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGE--IFIPEINQKFMVEPGKTRLFACQNPVSEGSGRKGLPKSFVNRFTQVYMSSLSDSDYRNILNCLYPA---IARETIGFIVEFVQNLREHVVVMKKFGTKGSPWEFNLRDLCR 1129
+G L QA+K G+WILLDE+NLA+ LECL+ L+ + + + + +V RLFAC NP ++ G++ LP NRFT++Y+ L + IL Y +++ T+ IV F LR+ GT P ++LR LCR
Sbjct: 816 EGTLAQAIKKGEWILLDEINLAAPETLECLSGLLEGSSGSLVLLDRGDTEPLVRHPDFRLFACMNPATD-VGKRNLPPGIRNRFTELYVEELESKEDLQILIVDYLKGLNVSKNTVQGIVNFYTALRKESGTKLVDGTGHRP-HYSLRTLCR 965 HSP 8 Score: 84.3445 bits (207), Expect = 3.995e-15 Identity = 62/185 (33.51%), Postives = 97/185 (52.43%), Query Frame = 1
Query: 1363 QLAILESMMLTVDMGWLTVLVGPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGSFE--QQKEGGK-------FKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGLVYG--DRIEEIKPHKNFRLFLILDPDCGG-----EISKAMRNRGLEI-CIIPENRE 1866
+LA+L L + G +LVG G GKTT+ ++ S + L S++ + M+T+D LG +QK K F+W D LV A+++ + +LD ++ SVL+RLN +LE L+L E+G + +E + K+FR+ ++P GG E+S A+RNR EI C RE
Sbjct: 1367 RLAVLVGRAL--EFGEPVLLVGDTGCGKTTVCQMFSALANQKLYSVNCHLNMETSDFLGGLRPVRQKPNDKDELDTRLFEWHDGPLVLAMKEDSFFLLDEISLADDSVLERLNSVLEVEKCLVLAEKGSPESKDNEVELLTAGKHFRILATMNP--GGDFGKKELSPALRNRFTEIWCPQSTKRE 1547 HSP 9 Score: 82.0333 bits (201), Expect = 1.774e-14 Identity = 51/166 (30.72%), Postives = 87/166 (52.41%), Query Frame = 1
Query: 1417 VLVGPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGS--FEQQKEGGKFKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGLVYGDRIEEIKPHKNFRLFLILDP--DCGGE--ISKAMRNRGLEICIIPENRELTLTDNDLLA 1896
+L G G GKT++V L+ +GN L ++++ D TDL G+ + GG+F W D L+ AL+ G W++LD +N S S+L+ LN + G + + E G+ + + H+ R+F +P GG + K+ NR ++ + P LT+ D + +A
Sbjct: 1745 LLEGSPGVGKTSLVAALAKASGNTLVRINLSEQTDITDLFGADLPVEGGRGGEFAWCDGPLLAALKAGHWVVLDELNLASQSILEGLNACFDHRGEIYVPELGMSFQVQ------HEKTRIFGCQNPFRQGGGRKGLPKSFLNRFTQVFVDP----LTVVDMEFIA 1900 HSP 10 Score: 81.2629 bits (199), Expect = 3.467e-14 Identity = 72/249 (28.92%), Postives = 118/249 (47.39%), Query Frame = 2
Query: 500 VSKKPLLLEGSPGVGKTSLVTQLAKLVGF----KVVRINLSEQTDANDLFGCFLPNENGKAGELSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPEINQKFMVEPGKTRLFACQNPVSEGSGRKGLP----KSFVNRF-TQVYMSSLSDSDYRNILNCLYPAIARET---IGFIVEFV---------------QNLREHVVVMKKFGTKGSPWEFNLRDLCRWCDLMVKHQHEP 1165
S+ +LLEG G GKTSLV LA + G +++++ L +QTD+ L G + + GE W+ G L QA G WILL++++ A V+ L L+H GE+ IP V P +LFA + +S G G P + ++++ T++++ +L+ D +L YP++ T + +E Q + E ++ +G E +LRDL WC+ V H +P
Sbjct: 320 ASQNAVLLEGPIGSGKTSLVEHLAAVTGRTKPPQLLKVQLGDQTDSKMLLGMYRCTD--VPGEFVWQPGTLTQAATKGYWILLEDIDYAPLDVVSVLIPLLEH-GELLIPGHGDCLKVAP-TFQLFATRRLLSCG-GSWYRPMNSHATMLDKYWTKIHLHNLNKKDLNEVLQSKYPSLLAATDHLLDIYIELTGEKHCCPSVAYDKAPQEVSEAERENRRVVLEGR--ELSLRDLLNWCN-RVAHGFDP 560 HSP 11 Score: 77.0258 bits (188), Expect = 6.778e-13 Identity = 50/161 (31.06%), Postives = 82/161 (50.93%), Query Frame = 1
Query: 1375 LESMMLTVDMGWLTVLV-GPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGSFEQQKEGGKFKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGLVYGDRIEEIKPHKNFRLFLILDPD--CGGE--ISKAMRNRGLEI 1842
L + V G VL+ G GKT++++ L+ +GNH ++ + D + +G + GK + + L+ A+R+G W++LD +N VL+ LN LL+ N LL+TE E ++ H F LF +P GG +S+A RNR +E+
Sbjct: 1062 LRDIARVVSAGTYPVLIQGETSVGKTSLIRWLAAASGNHCVRINNHEHTDIQEYIGCYTSDT-SGKLVFNEGVLIDAMRKGYWIVLDELNLAPTDVLEALNRLLDDNRELLITETQ-------EVVRAHPRFMLFATQNPPGLYGGRKVLSRAFRNRFVEL 1214 HSP 12 Score: 74.7146 bits (182), Expect = 3.191e-12 Identity = 72/242 (29.75%), Postives = 111/242 (45.87%), Query Frame = 2
Query: 488 RALQVSKKPLLLEGSPGVGKTSLVTQLAKLVGFKVVRINLSEQTDANDLFGCFLP--NENGKAGEL-----SWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPEI-------NQKFMVEPGKT-RLFACQNPVSEGSGRKGLPKSFVNRFTQVYM-SSLSDSDYRNILNC-LYPAIARETIGFIVEFVQNLREHVVVMKKFGTKGSPWEF------NLRDLCRWCDLM 1144
RAL+ + P+LL G G GKT++ + L K+ +N + +D G P + EL W DGPL+ A+K + LLDE++LA SVLE LN+ L+ + + E N+ ++ GK R+ A NP + G+K L + NRFT+++ S D I+N L P ++ +G V VM F T + EF ++RD+ W + M
Sbjct: 1374 RALEFGE-PVLLVGDTGCGKTTVCQMFSALANQKLYSVNCHLNMETSDFLGGLRPVRQKPNDKDELDTRLFEWHDGPLVLAMKEDSFFLLDEISLADDSVLERLNSVLEVEKCLVLAEKGSPESKDNEVELLTAGKHFRILATMNPGGD-FGKKELSPALRNRFTEIWCPQSTKREDLIQIINHNLRPGLSLAGVGHKGAGVAE------VMLAFITWLTRQEFGRKCVVSIRDILSWVNFM 1607 HSP 13 Score: 73.9442 bits (180), Expect = 4.574e-12 Identity = 58/179 (32.40%), Postives = 93/179 (51.96%), Query Frame = 1
Query: 7435 SLDTQKIL----DLFTKIKSFFCDLNMNQVNKFRNLINEMV--LPKLRSLEKVEKEFLGLLLNYHRVLISATDALLTVTTKLMKEGFCIPNDSAWNNEAENSNPSEKMNDFEMAGMDGGVGTNDVTDEIESESQIE-----GNNKDKKDETENQPDIPDEDDGIEMTDDFNGKMHDPEI 7938
+L QKI+ +L ++KS D N + F +V +P L + FL + L HR L T+L ++GFC+P + ++ E + + +D+E G+ G G DV+D IE+E Q+E G KD++D +++PD ED+ IEM++DF+GKMHD E+
Sbjct: 4559 TLHVQKIISSVSELLERLKSCSEDGNTTKHKVFSQSCGLLVRLIPILCGFSDLVLFFLTVSLATHRSTAKLLSVLAQTFTELSQKGFCLPKELMEDSAGEGAT---QFHDYEGGGIGDGEGMKDVSDRIENEEQVEDTFQKGQEKDEED-LDSKPDTKGEDNAIEMSEDFDGKMHDGEL 4733 HSP 14 Score: 63.5438 bits (153), Expect = 7.319e-9 Identity = 36/97 (37.11%), Postives = 59/97 (60.82%), Query Frame = 1
Query: 1570 FKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLE-PNGSLLLTERGLVYGDRIEEIKPHKNFRLFLILDP--DCGGE-ISKAMRNRGLEICI 1848
F +V+ TL QA+++G+W++LD +N + L+ L+GLLE +GSL+L +RG E + H +FRLF ++P D G + +RNR E+ +
Sbjct: 812 FAFVEGTLAQAIKKGEWILLDEINLAAPETLECLSGLLEGSSGSLVLLDRG-----DTEPLVRHPDFRLFACMNPATDVGKRNLPPGIRNRFTELYV 903 HSP 15 Score: 61.2326 bits (147), Expect = 3.195e-8 Identity = 43/145 (29.66%), Postives = 70/145 (48.28%), Query Frame = 1
Query: 1414 TVLVGPAGTGKTTIVKLLSDITGN----HLKSMSMNSAMDTTDLLGSFEQQKEGGKFKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGLVYGDRIEEIKPHKNFRLFLILD-PDCGGEISKAMRNRG 1833
+L GP G+GKT++V+ L+ +TG L + + D+ LLG + G+F W TL QA +G W++L+ +++ V+ L LLE +G LL+ G + +K F+LF CGG + M +
Sbjct: 325 VLLEGPIGSGKTSLVEHLAAVTGRTKPPQLLKVQLGDQTDSKMLLGMYRCTDVPGEFVWQPGTLTQAATKGYWILLEDIDYAPLDVVSVLIPLLE-HGELLIPGHG-------DCLKVAPTFQLFATRRLLSCGGSWYRPMNSHA 461 HSP 16 Score: 57.7658 bits (138), Expect = 3.974e-7 Identity = 48/166 (28.92%), Postives = 84/166 (50.60%), Query Frame = 2
Query: 635 FGC-FLPNENGKA-GELSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPEINQKFMVEPGKT---------RLFACQNPVSEGSGRKGLPKSFVNRFTQVYMSSLSDSDYRNILNCLYPAIARETIGFIVEFVQNLREHVVVMKKFGTKGSP 1099
FG L + +G++ G W D L+QALK+GDW+L+D +N + SVL+ LNA L+ G + IN++ MV+ G T RLF +P+ + ++ NR ++Y+S D ++ L+ + ++G + + V N+ + + +GSP
Sbjct: 2201 FGVKLLQSASGRSHGSFEWVDSMLVQALKSGDWLLMDNVNFCNPSVLDRLNALLEPGG---VLTINERGMVD-GSTCTVTPNPNFRLFLSMDPI-----HGEISRAMRNRGLEIYISGEEDGSIQDNLDL---KVLLHSLGLVGDSVCNILLALHTEIQSLVRGSP 2354 HSP 17 Score: 56.6102 bits (135), Expect = 8.953e-7 Identity = 27/59 (45.76%), Postives = 38/59 (64.41%), Query Frame = 1
Query: 1372 ILESMMLTVDMGWLTVLVGPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGSFE 1548
+LE + + V G +LVG GTGKT+ V+ L+ TG HL+ ++MN DT DLLG F+
Sbjct: 656 LLEQLAVCVSQGEPVLLVGETGTGKTSAVQYLAYATGQHLRVVNMNQQSDTADLLGGFK 714
BLAST of Midasin vs. UniProt/SwissProt
Match: sp|Q9NU22|MDN1_HUMAN (Midasin OS=Homo sapiens OX=9606 GN=MDN1 PE=1 SV=2) HSP 1 Score: 329.331 bits (843), Expect = 5.248e-107 Identity = 182/376 (48.40%), Postives = 234/376 (62.23%), Query Frame = 2
Query: 89 VVTIRDLLSWVNFINISIGNKTSLYQ--------SIFHGAYMVIIDSINDKLNKS-FYEELLIKK--LDYLVKSSFPDIVECLDFQSSRF---------ELSVKENKVGIDPFYIELGSHSDWXXXXXXXXXXKNFQYSFNSKTTGSNVYRLFRALQVSKKPLLLEGSPGVGKTSLVTQLAKLVGFKVVRINLSEQTDANDLFGCFLPNENGKAGELSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPEINQKFMVEPGKTRLFACQNPVSEGSGRKGLPKSFVNRFTQVYMSSLSDSDYRNILNCLYPAIARETIGFIVEFVQNLREHVVVMKKFGTKGSPWEFNLRDLCRWCDLMVKHQ 1156
VV+IRD+LSWVNF+N +G + +L + S H A +V ID I + S F LL +K L +L+K IV ++Q + E + +N GI PF+I G + + + Y+ ++ TT N RL RA ++ KKP+LLEGSPGVGKTSLV LAK G +VRINLSEQTD DLFG LP E GK GE +WRDGPLL ALKAG W++LDE+NLASQSVLE LNAC DHRGEI++PE+ F V+ KT++F CQNP +G GRKGLP+SF+NRFTQV++ L+ D I + L+PAI + + +V F + V V KK+G KG PWEFNLRDL RWC LM+ Q
Sbjct: 1599 VVSIRDILSWVNFMN-KMGEEAALKRPEIISTVTSFVHAACLVYIDGIGSGVTSSGFGTALLARKECLKFLIKR-LAKIVRLTEYQKNELKIYDRMKAKEFTGIDNLWGIHPFFIPRGP---------VLHRNNIADYALSAGTTAMNAQRLLRATKL-KKPILLEGSPGVGKTSLVGALAKASGNTLVRINLSEQTDITDLFGADLPVEGGKGGEFAWRDGPLLAALKAGHWVVLDELNLASQSVLEGLNACFDHRGEIYVPELGMSFQVQHEKTKIFGCQNPFRQGGGRKGLPRSFLNRFTQVFVDPLTVIDMEFIASTLFPAIEKNIVKKMVAFNNQIDHEVTVEKKWGQKGGPWEFNLRDLFRWCQLMLVDQ 1962 HSP 2 Score: 85.1149 bits (209), Expect = 5.248e-107 Identity = 51/135 (37.78%), Postives = 77/135 (57.04%), Query Frame = 1
Query: 1159 RTKEDQDACGKVYNELADKSRFARFIPSGLDFYITDQVLKIG--QTQLSVNIANNTDNSKQFNKH--LLLAGQLAILESMMLTVDMGWLTVLVGPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGSFEQ 1551
RT+ED+ V+ ++ S + Y+ ++ +I QL ++ + ++H LLL LES+M V M W+ +LVGPA GKT++V+LL+ +TG+ LK M+MNSAMDTT+LLG FEQ
Sbjct: 1982 RTEEDKKKVIAVFKDV---------FGSNSNPYMGTRLFRITPYDVQLGYSVLSRGSCVPHPSRHPLLLLHQSFQPLESIMKCVQMSWMVILVGPASVGKTSLVQLLAHLTGHTLKIMAMNSAMDTTELLGGFEQ 2107 HSP 3 Score: 234.187 bits (596), Expect = 1.378e-59 Identity = 127/283 (44.88%), Postives = 183/283 (64.66%), Query Frame = 1
Query: 9145 WSQYESKTEYLSRQLCEMLRIVLEPTKANKLGGAYRTGKRLNMKKVIPYIASGFRKDKIWLRRNKPSQREYQVIIGVDNSKSMAYNVNHQLFFESLSIVLNGLTFLEVGQVGLCKFGAKAEVVLPLYEKFSSEIGDRLIETFDFSDKCSKFIPILEQSIKLFEENQNFLSSSAVRXXXXXXXXXXXXXXXXXXXXPKFISLIREAHNKGIFVVVILLDHADSKDSILNLKMVIDDG----CDFVRFLDVFPLQNYTVLQDVTKLPTLLTDVLRQWFELSSSAD 9981
W Y T LS++LCE LR++LEPT+A KL G YRTGKRLN++KVIPYIAS FRKDKIWLRR KPS+R+YQ+ + +D+S SM N QL FESL+++ N LT LEVGQ+ +C FG +++ P +E+FS G +++ F K +K LE +F Q LS + +T+QLLL++SDG + ++ ++ A N IFV+ ++LD+ S+DSIL++K+ I G + +++ FP Y +L+DV LP L+D LRQWFEL +++D
Sbjct: 5313 WQSYLILTAPLSQRLCEELRLILEPTQAAKLKGDYRTGKRLNIRKVIPYIASQFRKDKIWLRRTKPSKRQYQICLAIDDSSSMVDNHTKQLAFESLAVIGNALTLLEVGQIAVCSFGESVKLLHPFHEQFSDYSGSQILRLCKFQQKKTKIAQFLESVANMFAAAQQ-LSQNISSETAQLLLVVSDGRGLFLEGKERVLAAVQAARNANIFVIFVVLDNPSSRDSILDIKVPIFKGPGEMPEIRSYMEEFPFPYYIILRDVNALPETLSDALRQWFELVTASD 5594 HSP 4 Score: 135.961 bits (341), Expect = 5.909e-30 Identity = 82/177 (46.33%), Postives = 109/177 (61.58%), Query Frame = 1
Query: 1564 GKFKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGLVYGDRIEEIKPHKNFRLFLILDPDCGGEISKAMRNRGLEICIIPENRELTLTDNDLLAILITIYKLSVKNADSLLRFHKTMILAQSETPQIKIG----NINSLIQAAKLI----KDGYSLDQSL 2070
G F+WVDS LVQAL+ GDWL++D VNFC+ SVLDRLN LLEP G L ++ERG++ G I P+ NFRLFL +DP G+IS+AMRNRGLEI I E T + DL +L ++ + D LL H +ET +G ++++LIQ A LI + G SLD++
Sbjct: 2220 GTFEWVDSMLVQALKSGDWLLMDNVNFCNPSVLDRLNALLEPGGVLTISERGMIDGS-TPTITPNPNFRLFLSMDP-VHGDISRAMRNRGLEIYISGEGDASTPDNLDLKVLLHSLGLVGNSVCDILLALH-------TETRSTVVGSPTSSVSTLIQTAILIVQYLQRGLSLDRAF 2387 HSP 5 Score: 127.872 bits (320), Expect = 1.962e-27 Identity = 78/226 (34.51%), Postives = 115/226 (50.88%), Query Frame = 2
Query: 470 NVYRLFRALQVSKKPLLLEGSPGVGKTSLVTQLAKLVGFKVVRINLSEQTDANDLFGCFLPNENGKAGELSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPEINQKFMVEPGKTRLFACQNPVSEGSGRKGLPKSFVNRFTQVYMSSLSDSDYRNILN---CLYPAIARETIGFIVEFVQNLREHVVVMKKFGTKGSPWEFNLRDLCRWCD 1138
N+ + R + P+L++G VGKTSL+ LA G VRIN E TD + GC+ + +GK L +++G L+ A++ G WI+LDE+NLA VLE LN LD E+ + E + P + LFA QNP GRK L ++F NRF +++ L S+ IL+ L P+ + + +++ R V K G LRDL RW +
Sbjct: 1065 NLRDIVRVVSAGTYPVLIQGETSVGKTSLIQWLAAATGNHCVRINNHEHTDIQEYIGCYTSDSSGK---LVFKEGVLIDAMRKGYWIILDELNLAPTDVLEALNRLLDDNRELLVTETQEVVKAHP-RFMLFATQNPPGLYGGRKVLSRAFRNRFVELHFDELPSSELETILHKRCSLPPSYCSKLVKVMLDLQSYRRSSSVFAGKQGF------ITLRDLFRWAE 1280 HSP 6 Score: 117.857 bits (294), Expect = 1.985e-24 Identity = 85/347 (24.50%), Postives = 173/347 (49.86%), Query Frame = 1
Query: 5191 EEKQDTTVFRDCYRFAAALISS--DNQGIPSDDCKLFAANLSALNWEYETLSPGVPQNTIGRP---HDIYQDPIDVDNLLQAQKILPNANVSVTKLLGIYPEHTTXXXXXXXXXXXXXXSGDYSLIVFINNVERLIHEIQQWEVVAARHVSLIQHVDELNGLLVSWRHQELTMWENTISTANWQLERAVSREFFNHYNLI---VQESNKIQYDNDFESINLILEPILNSLLNSTVGNFEYRLKTMKLSIESLISNREIDYRFKDRLKNILLNHYDFYRLYLGNVQEHLSSEYKRIGKELRDFIKTKRWGKTP--PKITDVLKCHKIVFKYKRQWSGIINQPCTKFFV 6201
E K ++F CY+ A+L++ G+ +D +L + L A + TL P + + +P +D YQ P +V Q Q +L + +V+ LL +PEH L + ++ I + + F+N +E L+ + Q WE A+R +SL +H+D ++ +++ WR EL W ++ + ++ +F+ Y ++ +QE + Q D+ ++ L++ + + S++G F RL+ + + ++ +++ KD L ++L N Y +Y+ + VQ + + KEL++F+K +W V K H+ +FK+ +++ ++++PC V
Sbjct: 3648 EAKHYLSLFLSCYQTGASLVTHFYPLMGVELND-RLLGSQLLACTLSHNTLFGEAPSDLMVKPDGPYDFYQHP-NVPEARQCQPVLQGFSEAVSHLLQDWPEHPALEQLLVVMDRIRSFPLSSPISKFLNGLEILLAKAQDWEENASRALSLRKHLDLISQMIIRWRKLELNCWSMSLDNTMKRHTEKSTKHWFSIYQMLEKHMQEQTEEQEDDKQMTLMLLVSTLQAFIEGSSLGEFHVRLQMLLVFHCHVLLMPQVEG--KDSLCSVLWNLYHYYKQFFDRVQAKIVELRSPLEKELKEFVKISKWNDVSFWSIKQSVEKTHRTLFKFMKKFEAVLSEPCRSSLV 3990 HSP 7 Score: 88.1965 bits (217), Expect = 1.932e-15 Identity = 62/176 (35.23%), Postives = 93/176 (52.84%), Query Frame = 1
Query: 1393 TVDMGWLTVLVGPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGSFE--QQKEGGK--------FKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGLV--YGDRIEEIKPHKNFRLFLILDPDCGG-----EISKAMRNRGLEI-CIIPENRE 1866
++ G +LVG G GKTTI ++ + + L S+S + M+T+D LG +QK K F+W D LVQA+++ + +LD ++ SVL+RLN +LE SL+L E+G IE + K FR+ ++P GG E+S A+RNR EI C +RE
Sbjct: 1379 ALEFGEPVLLVGDTGCGKTTICQVFAALANQKLYSVSCHLHMETSDFLGGLRPVRQKPNDKEEIDTSRLFEWHDGPLVQAMKEDGFFLLDEISLADDSVLERLNSVLEVEKSLVLAEKGSPEDKDSEIELLTAGKKFRILATMNP--GGDFGKKELSPALRNRFTEIWCPQSTSRE 1552 HSP 8 Score: 85.1149 bits (209), Expect = 1.430e-14 Identity = 52/152 (34.21%), Postives = 82/152 (53.95%), Query Frame = 2
Query: 689 DGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGE--IFIPEINQKFMVEPGKTRLFACQNPVSEGSGRKGLPKSFVNRFTQVYMSSLSDSDYRNILNCLY---PAIARETIGFIVEFVQNLREHVVVMKKFGTKGSPWEFNLRDLCR 1129
+G L QA+K G+WILLDE+NLA+ +LECL+ L+ + + + + +V RLFAC NP ++ G++ LP NRFT++Y+ L + +L Y ++ + T+ I+ F LR+ GT P ++LR LCR
Sbjct: 820 EGTLAQAVKKGEWILLDEINLAAPEILECLSGLLEGSSGSLVLLDRGDTEPLVRHPDFRLFACMNPATD-VGKRNLPPGIRNRFTELYVEELESKEDLQVLIVDYLKGLSVNKNTVQGIINFYTALRKESGTKLVDGTGHRP-HYSLRTLCR 969 HSP 9 Score: 79.7221 bits (195), Expect = 6.145e-13 Identity = 53/161 (32.92%), Postives = 83/161 (51.55%), Query Frame = 1
Query: 1375 LESMMLTVDMGWLTVLV-GPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGSFEQQKEGGKFKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGLVYGDRIEEIKPHKNFRLFLILDPD--CGGE--ISKAMRNRGLEI 1842
L ++ V G VL+ G GKT++++ L+ TGNH ++ + D + +G + GK + + L+ A+R+G W+ILD +N VL+ LN LL+ N LL+TE E +K H F LF +P GG +S+A RNR +E+
Sbjct: 1066 LRDIVRVVSAGTYPVLIQGETSVGKTSLIQWLAAATGNHCVRINNHEHTDIQEYIGCYTSDS-SGKLVFKEGVLIDAMRKGYWIILDELNLAPTDVLEALNRLLDDNRELLVTETQ-------EVVKAHPRFMLFATQNPPGLYGGRKVLSRAFRNRFVEL 1218 HSP 10 Score: 79.337 bits (194), Expect = 9.108e-13 Identity = 50/166 (30.12%), Postives = 88/166 (53.01%), Query Frame = 1
Query: 1417 VLVGPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGS--FEQQKEGGKFKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGLVYGDRIEEIKPHKNFRLFLILDP--DCGGE--ISKAMRNRGLEICIIPENRELTLTDNDLLA 1896
+L G G GKT++V L+ +GN L ++++ D TDL G+ + +GG+F W D L+ AL+ G W++LD +N S SVL+ LN + G + + E G+ + + H+ ++F +P GG + ++ NR ++ + P LT+ D + +A
Sbjct: 1750 LLEGSPGVGKTSLVGALAKASGNTLVRINLSEQTDITDLFGADLPVEGGKGGEFAWRDGPLLAALKAGHWVVLDELNLASQSVLEGLNACFDHRGEIYVPELGMSFQVQ------HEKTKIFGCQNPFRQGGGRKGLPRSFLNRFTQVFVDP----LTVIDMEFIA 1905 HSP 11 Score: 77.0258 bits (188), Expect = 3.782e-12 Identity = 66/241 (27.39%), Postives = 112/241 (46.47%), Query Frame = 2
Query: 500 VSKKPLLLEGSPGVGKTSLVTQLAKLVGF----KVVRINLSEQTDANDLFGCFLPNENGKAGELSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPEINQKFMVEPGKTRLFACQNPVSEGSGRKGLP----KSFVNRF-TQVYMSSLSDSDYRNILNCLYPAIARETIGFIVEFVQ--NLREHVVVMKKFGTKGSP-----------------WEFNLRDLCRWCD 1138
S+ +LLEG G GKTSLV LA + G +++++ L +QTD+ L G + + GE W+ G L QA G WILL++++ A V+ L L++ GE+ IP V PG + FA + +S G G P + ++++ T++++ +L + +L YP++ + ++Q + H G + +P E +LRDL WC+
Sbjct: 320 ASQNAVLLEGPIGCGKTSLVEYLAAVTGRTKPPQLLKVQLGDQTDSKMLLGMYRCTD--VPGEFVWQPGTLTQAATMGHWILLEDIDYAPLDVVSVLIPLLEN-GELLIPGRGDCLKVAPG-FQFFATRRLLSCG-GNWYRPLNSHATLLDKYWTKIHLDNLDKRELNEVLQSRYPSLLAVVDHLLDIYIQLTGEKHHSWSDSSVGCEQAPEEVSEARRENKRPTLEGRELSLRDLLNWCN 555 HSP 12 Score: 75.0998 bits (183), Expect = 1.651e-11 Identity = 71/259 (27.41%), Postives = 114/259 (44.02%), Query Frame = 2
Query: 488 RALQVSKKPLLLEGSPGVGKTSLVTQLAKLVGFKVVRINLSEQTDANDLFGCFLP--------NENGKAGELSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPEINQ--------KFMVEPGKTRLFACQNPVSEGSGRKGLPKSFVNRFTQVYM-SSLSDSDYRNIL-NCLYPAIARETIG--------FIVEFVQNLREHVVVMKKFGTKGSPWEFNLRDLCRWCDLMVKHQHEPRKTRTPV 1186
RAL+ + P+LL G G GKT++ A L K+ ++ + +D G P E + W DGPL+QA+K + LLDE++LA SVLE LN+ L+ + + E + + K R+ A NP + G+K L + NRFT+++ S S D I+ + L P + I +++F+ L ++FG K ++RD+ W + M K E R +
Sbjct: 1378 RALEFGE-PVLLVGDTGCGKTTICQVFAALANQKLYSVSCHLHMETSDFLGGLRPVRQKPNDKEEIDTSRLFEWHDGPLVQAMKEDGFFLLDEISLADDSVLERLNSVLEVEKSLVLAEKGSPEDKDSEIELLTAGKKFRILATMNPGGD-FGKKELSPALRNRFTEIWCPQSTSREDLIQIISHNLRPGLCLGRIDPKGSDIPEVMLDFIDWL-----THQEFGRKCV---VSIRDILSWVNFMNKMGEEAALKRPEI 1626 HSP 13 Score: 72.4034 bits (176), Expect = 1.182e-10 Identity = 84/328 (25.61%), Postives = 150/328 (45.73%), Query Frame = 1
Query: 7063 KSYLVNMKHMECLSQQKLIIKGINELIEIVKNRFSPENKKINSSITESLLKFNDSNERAIIEIET-SLHVANDNFMDTDDSFD-GFVNELA----VVIREKISAFKTI------LLTDNVDKS--------FIKVNT-HINALEQ--------SLDTQKIL----DLFTKIKSFFCDLNMNQVNKFRNLINEMV--LPKLRSLEKVEKEFLGLLLNYHRVLISATDALLTVTTKLMKEGFCIPNDSAWNNEAENSNPSEKMNDFEMAGMDGGVGTNDVTDEIESESQIE-----GNNKDKKDETENQPDIPDEDDGIEMTDDFNGKMH 7926
K + V + CLSQ + ++G+ L + E + ++ ESL +A+ + T H+ + + D GFV + + + IR + A + + +N D++ F ++ + H+ L + +L QKI+ +L ++KS+ D + F + +V +P L S + FL + L HR L V T+L ++GFC+P + ++ E + + +D+E G+ G G DV+D+I +E Q+E G KDK+D +++ DI ED+ IEM++DF+GKMH
Sbjct: 4417 KDFEVCSSALSCLSQVSVHLQGLESLF--ILPGMEVEQRDSQMALVESLEYVRGEISKAMADFTTWKTHLLTSDSQGGNQMLDEGFVEDFSEQMEIAIRAILCAIQNLEERKNEKAEENTDQASPQEDYAGFERLQSGHLTKLLEDDFWADVSTLHVQKIISAISELLERLKSYGEDGTAAKHLFFSQSCSLLVRLVPVLSSYSDLVLFFLTMSLATHRSTAKLLSVLAQVFTELAQKGFCLPKEFMEDSAGEGAT---EFHDYEGGGIGEGEGMKDVSDQIGNEEQVEDTFQKGQEKDKED-PDSKSDIKGEDNAIEMSEDFDGKMH 4738 HSP 14 Score: 66.6254 bits (161), Expect = 5.428e-9 Identity = 45/158 (28.48%), Postives = 76/158 (48.10%), Query Frame = 1
Query: 1270 VLKIGQTQLSVNIANNTDNSKQ----FNKHLLLAGQLAILESMMLTVDMGWLTVLVGPAGTGKTTIVKLLSDITGN----HLKSMSMNSAMDTTDLLGSFEQQKEGGKFKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERG 1719
V+ GQ + N +S++ ++L+ L+++ + V +L GP G GKT++V+ L+ +TG L + + D+ LLG + G+F W TL QA G W++L+ +++ V+ L LLE NG LL+ RG
Sbjct: 273 VVLPGQLPAPGELGGNRSSSREQELALRSYVLVESVCKSLQTLAMAVASQNAVLLEGPIGCGKTSLVEYLAAVTGRTKPPQLLKVQLGDQTDSKMLLGMYRCTDVPGEFVWQPGTLTQAATMGHWILLEDIDYAPLDVVSVLIPLLE-NGELLIPGRG 429 HSP 15 Score: 64.3142 bits (155), Expect = 3.072e-8 Identity = 36/97 (37.11%), Postives = 60/97 (61.86%), Query Frame = 1
Query: 1570 FKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLE-PNGSLLLTERGLVYGDRIEEIKPHKNFRLFLILDP--DCGGE-ISKAMRNRGLEICI 1848
F +V+ TL QA+++G+W++LD +N + +L+ L+GLLE +GSL+L +RG E + H +FRLF ++P D G + +RNR E+ +
Sbjct: 816 FAFVEGTLAQAVKKGEWILLDEINLAAPEILECLSGLLEGSSGSLVLLDRG-----DTEPLVRHPDFRLFACMNPATDVGKRNLPPGIRNRFTELYV 907
BLAST of Midasin vs. UniProt/SwissProt
Match: sp|A0A1P8AUY4|MDN1_ARATH (Midasin OS=Arabidopsis thaliana OX=3702 GN=MDN1 PE=1 SV=1) HSP 1 Score: 293.508 bits (750), Expect = 4.884e-91 Identity = 167/384 (43.49%), Postives = 225/384 (58.59%), Query Frame = 2
Query: 89 VVTIRDLLSWVNFINISIGNKTSLYQSIFHGAYMVIIDSIN-----------DKLNKSFYEELLIKKLDYLVKSSFPDIVECLDFQSSRFEL--------------SVK-ENKVGIDPFYIELGSHSDWXXXXXXXXXXKNFQYSFNSKTTGSNVYRLFRALQVSKKPLLLEGSPGVGKTSLVTQLAKLVGFKVVRINLSEQTDANDLFGCFLPNENGKAGELSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPEINQKFMVEPGKTRLFACQNPVSEGSGRKGLPKSFVNRFTQVYMSSLSDSDYRNILNCLYPAIARETIGFIVEFVQNLREHVVVMKKFGTKGSPWEFNLRDLCRWCDLMVKHQHE 1162
+T+RDLLSWV F+N++ + Y +I HGA++V++D ++ D K F L+++L+ + P + SR EL SV+ E GIDPF+I G + + F + TT NV R+ RA+Q+SK P+LLEGSPGVGKTSL+ L K G KVVRINLSEQTD DL G LP E+ + + +W DG LLQALK G W+LLDE+NLA QSVLE LNA LDHR ++FIPE+ F P R+FACQNP ++G GRKGLPKSF+NRFT+VY+ L + DY I LYP++ + ++ + L + ++ +KFG GSPWEFNLRD+ R C M + H+
Sbjct: 1636 TLTVRDLLSWVAFVNMATESLGPAY-AILHGAFLVLLDGLSLGTGFSGRDGQDLREKCF--AFLLQQLELFASDTLP-------LELSRMELYGWGDSKAICEKSKSVRHEGMFGIDPFFISKGDENPEIGG-----------FEFLAPTTHRNVLRVLRAMQLSK-PILLEGSPGVGKTSLILALGKYSGHKVVRINLSEQTDMMDLLGSDLPVESDEDMKFAWSDGILLQALKEGSWVLLDELNLAPQSVLEGLNAILDHRAQVFIPELGCTFECPP-TFRVFACQNPSTQGGGRKGLPKSFLNRFTKVYVDELVEDDYLFICRSLYPSVPSPLLSKLIALNRQLHDGTMLYRKFGHDGSPWEFNLRDVIRSCQFMQEAIHD 1996 HSP 2 Score: 67.781 bits (164), Expect = 4.884e-91 Identity = 42/141 (29.79%), Postives = 72/141 (51.06%), Query Frame = 1
Query: 1159 RTKEDQDACGKVYNELADKSRFARFIPSGLDFYITDQVLKIGQTQLSVNIANNTDNSKQFNKHLLLAGQLAILESMMLTVDMGWLTVLVGPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGSFEQQKEGGKFKWV 1581
RT D+ ++Y + DK+ P + L +G + N+ + S+Q LL LE++ V WL +LVGP+ +GKT+++++L+ +TG L ++++SA D++DLLG FEQ F+ V
Sbjct: 2012 RTATDRKEVLRIYKAIFDKTPSINPYPR---VQLNPAYLVVGTAAIKRNLNQSNIASEQLK---LLPEIRQNLEAVAHCVQNKWLCILVGPSSSGKTSVIRILAQLTGYPLNELNLSSATDSSDLLGCFEQYNAFRNFRLV 2146 HSP 3 Score: 166.007 bits (419), Expect = 5.032e-39 Identity = 107/290 (36.90%), Postives = 157/290 (54.14%), Query Frame = 1
Query: 9145 WSQYESKTEYLSRQLCEMLRIVLEPTKANKLGGAYRTGKRLNMKKVIPYIASGFRKDKIWLRRNKPSQREYQVIIGVDNSKSMAYNVNHQLFFESLSIVLNGLTFLEVGQVGLCKFGAKAEVVLP--LYEKFSSEIGDRLIETFDFSDKCSKFIPILEQSIKLFEENQNFLSSSAVRXXXXXXXXXXXXXXXXXXXXPKFI------SLIREAHNKGIFVVVILLDHADSKDSILNLKMVIDDG----CDFVRFLDVFPLQNYTVLQDVTKLPTLLTDVLRQWFELSSSA 9978
W + E T S++L E LR++LEPT A+KL G YRTGKR+NMKKVIPYIAS +RKDKIWLRR KP++R+YQV+I VD+S+SM+ + +L+ V ++ LE+G + + FG + + + + F++E G ++I F + I +Q + N N + + Q L + D KF +R + VV +LLD D++ S+ +L + DG + +LD FP Y VL+D+ LP L DVLRQWFEL S+
Sbjct: 5115 WRRCELLTAKPSQELAEQLRLILEPTLASKLSGDYRTGKRINMKKVIPYIASHYRKDKIWLRRTKPNKRDYQVVIAVDDSRSMSESGCGDFAIRALATVCRAMSQLELGSLAVASFGKQGSIKMLHDFGQSFTTESGIKMISNLTFKQENL----IEDQPVVNLLRNMNEMLENLASTRRQSYGSNPLQQLVLIIGDGKFHEREKLKRTVRSFLQQKRMVVYLLLD--DAEQSVFDLADYVYDGERRPYKKMNYLDSFPFPYYIVLRDIEALPRTLGDVLRQWFELMQSS 5398 HSP 4 Score: 137.887 bits (346), Expect = 1.540e-30 Identity = 82/228 (35.96%), Postives = 127/228 (55.70%), Query Frame = 2
Query: 452 SKTTGSNVYRLFRALQVSKKPLLLEGSPGVGKTSLVTQLAKLVGFKVVRINLSEQTDANDLFGCFLPNENGKAGELSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPEINQKFMVEPGKTRLFACQNPVSEGSGRKGLPKSFVNRFTQVYMSSLSDSDYRNILNCLYPAIARETIGFIVEFVQNLREHVVVMKKFGTKGSPWEFNLRDLCRWC 1135
+K+ ++ L A+ + + P+LL+G GKTSLV LA + G K VRIN EQTD + G ++ + +GK L + +G L++A++ G WI+LDE+NLA VLE LN LD E+F+PE+++ P LFA QNP + GRK L ++F NRF ++++ + + + IL +IA +VE +++L+ + K F G RDL RW
Sbjct: 1054 TKSVIDHLNHLAHAIFIKRYPVLLQGPTSSGKTSLVKYLAAISGNKFVRINNHEQTDIQEYLGSYMTDSSGK---LVFHEGALVKAVRGGHWIVLDELNLAPSDVLEALNRLLDDNRELFVPELSETISAHPN-FMLFATQNPPTLYGGRKILSRAFRNRFVEIHVDEIPEDELSEILTT-KCSIANSHASKMVEVMKDLQRNRQSSKAFA--GKHGYITPRDLFRWA 1274 HSP 5 Score: 104.76 bits (260), Expect = 1.795e-20 Identity = 46/107 (42.99%), Postives = 77/107 (71.96%), Query Frame = 1
Query: 1549 QQKEGGKFKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGLVYGDRIEEIKPHKNFRLFLILDPDCGGEISKAMRNRGLEICIIPENREL 1869
++K+ KF+WV L++A+ +G+W++L N C+ +VLDR+N L+EP GS+ + E G+V G+ + + PH NFRLFL ++P GE+S+AMRNRG+E+ ++ + +L
Sbjct: 2247 EKKQSTKFEWVTGMLIKAIEKGEWVVLKNANLCNPTVLDRINSLVEPCGSITINECGIVNGEPV-TVVPHPNFRLFLSVNPKF-GEVSRAMRNRGVEVFMMGPHWQL 2351 HSP 6 Score: 88.5817 bits (218), Expect = 1.487e-15 Identity = 56/155 (36.13%), Postives = 84/155 (54.19%), Query Frame = 1
Query: 1414 TVLVGPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGSFEQQKEGGKFKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGLVYGDRIEEIKPHKNFRLFLILDPDC--GGE--ISKAMRNRGLEICI--IPEN 1860
+L GP +GKT++VK L+ I+GN ++ + D + LGS+ GK + + LV+A+R G W++LD +N + VL+ LN LL+ N L + E E I H NF LF +P GG +S+A RNR +EI + IPE+
Sbjct: 1075 VLLQGPTSSGKTSLVKYLAAISGNKFVRINNHEQTDIQEYLGSY-MTDSSGKLVFHEGALVKAVRGGHWIVLDELNLAPSDVLEALNRLLDDNRELFVPELS-------ETISAHPNFMLFATQNPPTLYGGRKILSRAFRNRFVEIHVDEIPED 1221 HSP 7 Score: 77.7962 bits (190), Expect = 2.421e-12 Identity = 55/189 (29.10%), Postives = 94/189 (49.74%), Query Frame = 1
Query: 1366 LAILESMMLTVDMGWLTVLVGPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGS--FEQQKEGGKFKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGLVYGDRIEEIKPHKNFRLFLILDPDCGG----EISKAMRNRGLEICIIPENRELTLTDNDLLAILITIY 1914
L +L +M L+ + +L G G GKT+++ L +G+ + ++++ D DLLGS + E KF W D L+QAL++G W++LD +N SVL+ LN +L+ + + E G + E P FR+F +P G + K+ NR ++ + L ++D L I ++Y
Sbjct: 1770 LRVLRAMQLSKPI----LLEGSPGVGKTSLILALGKYSGHKVVRINLSEQTDMMDLLGSDLPVESDEDMKFAWSDGILLQALKEGSWVLLDELNLAPQSVLEGLNAILDHRAQVFIPELGCTF-----ECPP--TFRVFACQNPSTQGGGRKGLPKSFLNRFTKVYVD------ELVEDDYLFICRSLY 1941 HSP 8 Score: 76.2554 bits (186), Expect = 6.614e-12 Identity = 51/173 (29.48%), Postives = 89/173 (51.45%), Query Frame = 1
Query: 1375 LESMMLTVDMGWLTVLVGPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGSFEQQKEGGKFKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGLVYGDRIEEIKPHKNFRLF-LILDPDC--------GGEISKAMRNRGLEICIIPENRE 1866
E + L V +L GP+G+GK+ +++ L+D +GNH+ + M+ +D L+G++ + G+F+W +L QA+ G W++L+ ++ + V L+ LL + S LT +G EEI+ + F+LF I P+C G +S R I + P +RE
Sbjct: 343 FEMVSLAVSQKRPVLLYGPSGSGKSALIRKLADESGNHVVFIHMDDQLDGKTLVGTYVCTDQPGEFRWQPGSLTQAIMNGFWVVLEDIDKAPSDVPLVLSSLLGGSCS-FLTSQG-------EEIRIAETFQLFSTISTPECSVSHIRDAGNSLSPLWRR----IVVYPPDRE 503 HSP 9 Score: 71.633 bits (174), Expect = 1.999e-10 Identity = 60/223 (26.91%), Postives = 102/223 (45.74%), Query Frame = 2
Query: 491 ALQVS-KKPLLLEGSPGVGKTSLVTQLAKLVGFKVVRINLSEQTDANDLFGCFLPNENGKAGELSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPEINQKFMVEPGKTRLFAC-QNPVSEGSGRKGLPKSFVNRFTQVYMSSLSDSDYRNILNCLYP---AIARETIGFIVEFVQNLREHV--VVMKKFGTKGSPWEFNLRDLCRWCD 1138
+L VS K+P+LL G G GK++L+ +LA G VV I++ +Q D L G ++ + + GE W+ G L QA+ G W++L++++ A V L++ L + + + E +LF+ P S + S + ++ + ++IL YP +A + I LR + T SP F+LRDL +WC+
Sbjct: 347 SLAVSQKRPVLLYGPSGSGKSALIRKLADESGNHVVFIHMDDQLDGKTLVGTYVCTD--QPGEFRWQPGSLTQAIMNGFWVVLEDIDKAPSDVPLVLSSLLGGSCSFLTSQGEEIRIAE--TFQLFSTISTPECSVSHIRDAGNSLSPLWRRIVVYPPDRESLQSILGARYPNLGPVAEKLIETFETINSALRPQFSSSTTENSATFSSPSRFSLRDLLKWCE 565 HSP 10 Score: 68.1662 bits (165), Expect = 1.934e-9 Identity = 39/104 (37.50%), Postives = 62/104 (59.62%), Query Frame = 1
Query: 1552 QKEGGKFKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGLVYGDRIEEIKPHKNFRLFLILDP--DCG-GEISKAMRNRGLEICIIP 1854
QK F W D LV+A+R G+ +++D ++ SVL+R+N +LE + L L E+G G +EE+ H++F + ++P D G E+S A+RNR EI + P
Sbjct: 1494 QKWRAIFVWQDGPLVEAMRAGNIVLVDEISLADDSVLERMNSVLETDRKLSLAEKG---GPVLEEVVAHEDFFVLATMNPGGDYGKKELSPALRNRFTEIWVPP 1594 HSP 11 Score: 64.6994 bits (156), Expect = 2.231e-8 Identity = 49/141 (34.75%), Postives = 71/141 (50.35%), Query Frame = 1
Query: 1546 EQQKEGGK-FKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEP-NGSLLLTERGLVYGDRIEEIKPHKNFRLFLILDP--DCGG-EISKAMRNRGLEICIIPENRELTL------------TDNDLLAILITIYK 1917
+Q GG F +V+ V ALR+G W++LD VN +L RL G+LE GSL L ERG V G I H NFRLF ++P D G ++ + R+R E + + + L +D+ L+A ++ YK
Sbjct: 816 QQIHSGGMVFTFVEGAFVTALREGHWVLLDEVNLAPPEILGRLIGVLEGVRGSLCLAERGDVMG-----IPRHLNFRLFACMNPATDAGKRDLPFSFRSRFTEYAVDDDICDDDLEIFVRRFLGGRGSDSKLVANIVWFYK 951 HSP 12 Score: 61.6178 bits (148), Expect = 2.052e-7 Identity = 33/99 (33.33%), Postives = 61/99 (61.62%), Query Frame = 2
Query: 683 WRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPEINQKFMVEPGKTRLF---ACQNPVSEGSGRKGLPKSFVNRFTQVYMSSLSDSD 970
W+DGPL++A++AG+ +L+DE++LA SVLE +N+ L+ ++ + E + E F A NP + G+K L + NRFT++++ ++D++
Sbjct: 1502 WQDGPLVEAMRAGNIVLVDEISLADDSVLERMNSVLETDRKLSLAEKGGPVLEEVVAHEDFFVLATMNPGGD-YGKKELSPALRNRFTEIWVPPITDTE 1599 HSP 13 Score: 57.3806 bits (137), Expect = 3.200e-6 Identity = 34/93 (36.56%), Postives = 51/93 (54.84%), Query Frame = 2
Query: 689 DGPLLQALKAGDWILLDEMNLASQSVLECL---------NACLDHRGEIFIPEINQKFMVEPGKTRLFACQNPVSEGSGRKGLPKSFVNRFTQ 940
+G + AL+ G W+LLDE+NLA +L L + CL RG++ + F RLFAC NP ++ +G++ LP SF +RFT+
Sbjct: 829 EGAFVTALREGHWVLLDEVNLAPPEILGRLIGVLEGVRGSLCLAERGDVMGIPRHLNF-------RLFACMNPATD-AGKRDLPFSFRSRFTE 913
BLAST of Midasin vs. UniProt/SwissProt
Match: sp|O94248|MDN1_SCHPO (Midasin OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=mdn1 PE=1 SV=1) HSP 1 Score: 263.077 bits (671), Expect = 3.664e-84 Identity = 152/361 (42.11%), Postives = 210/361 (58.17%), Query Frame = 2
Query: 89 VVTIRDLLSWVNFINISIGNKTSLYQSIFHGAYMVIIDSINDKLNKSFYEELL---------IKKLDYLVKSSFPDIVECLDFQSSRFELSVKENKVGIDPFYIELGSHSDWXXXXXXXXXXKNFQYSFNSKTTGSNVYRLFRALQVSKKPLLLEGSPGVGKTSLVTQLAKLVGFKVVRINLSEQTDANDLFGCFLPNENGKAGELSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPEINQKFMVEPGKTRLFACQNPVSEGSGRKGLPKSFVNRFTQVYMSSLSDSDYRNILNCLYPAIARETIGFIVEFVQNLREHVVVMKKFGTKGSPWEFNLRDLCRWCDLM 1144
V++IRD+LS V FIN L +F+ MV ID++ S L KL+ L S+ + +S+ + ++ I F I LG + + YS ++ TT N ++ RALQV KP+LLEGSPGVGKTSL+T LA+ G ++VRINLS+QTD DLFG +P E G+ G+ +WRD P L A++ G W+LLDE+NLASQSVLE LNACLDHR E +IPE+++ F P R+FA QNP +G GRKGLP+SF+NRF+ VY+ +L + D I C Y + + I++F+ L++++ FG+ GSPWEFNLRD RW L+
Sbjct: 1431 VISIRDVLSAVEFINAC--EILDLNLVLFNAVSMVFIDALGSFTTFSLSNNLASLHAERQRCFAKLNELAGSNI------MASKSADISIKFSDSSFFIGDFGIPLGDSVE-----------SDSTYSLHTDTTLMNASKVLRALQV-LKPILLEGSPGVGKTSLITALARETGHQLVRINLSDQTDLMDLFGSDVPVEGGEGGQFAWRDAPFLAAMRNGHWVLLDELNLASQSVLEGLNACLDHRNEAYIPELDKVFKAHPN-FRVFAAQNPQHQGGGRKGLPRSFINRFSVVYVEALKEKDMIEIAACNYHQVNEDWRLKIIKFMFRLQDNIEKDISFGSFGSPWEFNLRDTLRWLQLL 1770 HSP 2 Score: 75.0998 bits (183), Expect = 3.664e-84 Identity = 42/131 (32.06%), Postives = 71/131 (54.20%), Query Frame = 1
Query: 1159 RTKEDQDACGKVYNELADKSRFARFIPSGLDFYITDQVLKIGQTQLSVNIANNTDNSKQFNKHLLLAGQLAILESMMLTVDMGWLTVLVGPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGSFEQ 1551
RT ED+ +++ E+ D + P + F ++ Q K+G + L ++ + +L QL +LES++ ++ W +LVG TGKT I++LL+ I G +K M++NS DT DL+G +EQ
Sbjct: 1794 RTVEDRVRTCELFKEVFD----IDYEPRTIGFSLSSQCFKVGHSLLVRDVERQ---KTLLDSQNILQSQLPVLESVITCINKKWPCILVGDTATGKTCILRLLAAIAGAKIKEMAVNSDTDTMDLIGEYEQ 1917 HSP 3 Score: 197.978 bits (502), Expect = 1.183e-48 Identity = 110/285 (38.60%), Postives = 174/285 (61.05%), Query Frame = 1
Query: 9136 RFTWSQYESKTEYLSRQLCEMLRIVLEPTKANKLGGAYRTGKRLNMKKVIPYIASGFRKDKIWLRRNKPSQREYQVIIGVDNSKSMAYNVNHQLFFESLSIVLNGLTFLEVGQVGLCKFGAKAEVVLPLYEKFSSEIGDRLIETFDFSDKCSKFIPILEQSIKLFEENQNFLSSSAVRXXXXXXXXXXXXXXXXXXXXPKFISLIREAHNKGIFVVVILLDHADS--KDSILNLKMVI----DDGCDFVR---FLDVFPLQNYTVLQDVTKLPTLLTDVLRQWFE 9963
R W+++E T+ LS +LCE LR++LEPT A K+ G +RTGKRLNMK++IPYIAS F+KDKIW+RR KPS+R YQV+I +D+SKSM+ + + L E+L++V L+ LEVGQ+ + KFG + E++ P ++FSSE G ++ F F + + + + S+K F ++S R S + L L+R A + + +V ++LD+ ++ K SIL++K V +DG ++ ++D F Y V++++ +LP LL+ LRQWF+
Sbjct: 4431 RDLWNKHEDSTKQLSIELCEQLRLILEPTLATKMQGDFRTGKRLNMKRIIPYIASQFKKDKIWMRRVKPSKRTYQVMISIDDSKSMSESGSTVLALETLALVTKALSLLEVGQIAVMKFGEQPELLHPFDKQFSSESGVQMFSHFTFEQSNTNVLALADASMKCFNYAN---TASHHRSNSDIRQLEIIISDGICEDHDSIRKLLRRAQEEKVMIVFVILDNVNTQKKSSILDIKKVYYDTKEDGTMDLKIQPYIDEFAFDYYLVVRNIEELPQLLSSALRQWFQ 4712 HSP 4 Score: 135.576 bits (340), Expect = 7.239e-30 Identity = 85/225 (37.78%), Postives = 123/225 (54.67%), Query Frame = 2
Query: 470 NVYRLFRALQVSKKPLLLEGSPGVGKTSLVTQLAKLVGFKVVRINLSEQTDANDLFGCFLPNENGKAGELSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPEINQKFMVEPGKT-RLFACQNPVSEGSGRKGLPKSFVNRFTQVYMSSLSDSDYRNILN--CLYPAIARETIGFIVEFVQNLREHVVVMKKFGTKGSPWEFNLRDLCRWC 1135
N+ + RA P+L++G GKTS++ +AK G K VRIN E TD + G ++ ++NG LS+R+G L++AL+ G WI+LDE+NLA VLE LN LD E+FIPE + +V+P LFA QNP +GRK L ++F NRF +++ + +++ IL+ C IA IV+ + L + F K S LRDL RW
Sbjct: 882 NLLNIARACSTRMFPILIQGPTSSGKTSMIEYVAKKTGHKFVRINNHEHTDLQEYIGTYVTDDNGS---LSFREGVLVEALRNGYWIVLDELNLAPTDVLEALNRLLDDNRELFIPET--QVLVKPHPEFMLFATQNPPGVYAGRKHLSRAFRNRFLEIHFDDIPENELETILHKRC---KIAPSYAAKIVQVFRELSLRRQTTRIFEQKNSFA--TLRDLFRWA 1096 HSP 5 Score: 105.916 bits (263), Expect = 8.262e-21 Identity = 56/124 (45.16%), Postives = 78/124 (62.90%), Query Frame = 1
Query: 1531 LLGSFEQQKEGGKFKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGLVYGDRIEEIKPHKNFRLFLILDPDCGGEISKAMRNRGLEICIIPENRELTLTDNDLLAIL 1902
LL F G+F+W D L++A+ +G W +LD N CS +VLDRLN LLE G L++ E+ G + IKPH NFRLFL ++P GE+S+AMRNRG+EI ++ E LT D +++L
Sbjct: 2002 LLAEFSASP-AGRFEWFDGYLLKAVEEGHWFVLDNANLCSPAVLDRLNSLLEHKGVLIVNEKTTEDG-HPKTIKPHPNFRLFLTVNP-VYGELSRAMRNRGVEIFLLKE--ALTEIDKKQMSLL 2120 HSP 6 Score: 83.1889 bits (204), Expect = 5.085e-14 Identity = 200/942 (21.23%), Postives = 373/942 (39.60%), Query Frame = 1
Query: 5374 HDIYQDP-----IDVDNLLQAQKILPNANVSVTKLLGIYPEHTTXXXXXXXXXXXXXXSGDYSLIVFINNVERLIHEIQQWEVVAARHVSLIQHVDELNGLLVSWRHQELTMWENTISTANWQLERAVSREFFNHYNLIVQ----ESNKIQYDNDFESINLILEPILNSLLNSTVGNFEYRLKTMKLSIESLISNREIDYRFKDRLKNILLNHYDFYRLYLGNVQEHLSSEYKRIGKELRDFIKTKRWGKTPPKIT--DVLKCHKIVFKYKRQWSGIINQPCTKFFVL-------------TPINALKQVNLIDIGNCIES-DIDFSK---SKLSDWCATMYEYSREMWETMENFQKETKELEDISVVQSKSEDDKEALEKKWRM-------NVKNLQQRKRNCVSQFVKELREMGL---LAHG-----SDLPRNTALMTHLPLGDIENSQLPFSTIRCLRL-------------RLEVDDSDKS------------EEFAKFNDFKNRLISICDHLLSIACTNTTKIKHFQ----NEFNIIG------NSIEYLSNTCQSEIIAIQDPTDIERNFSLMTSNNSEKINQWDLFIEILKGATCSRRFPSLLTDDLKPSLLTSFDDRDFLKSLTKQICQVNVPKSYLVNMKHMECLSQQKLIIKGINELIEIVKNRFSPENKKINSSITESLLKFNDSNERAIIEIETSLHVANDNFMDTDDSFDGFVNELAVVIREKISAFKTILLTDNVDKSFIKVNTHINALEQSLDTQKILDLF------TKIKSFFCDLNMNQVNKFRNLINEMVLPKLRSLEKVEKEFLGLLLNYHRVLISATDALLTVTTKLMKEGFCIPNDSAWNNEAENSNPSE-KMNDFEM---AGMDGGVGTNDVTDEIESESQIE--GNNKDKKDETENQPDIPDEDDGIEMTDDFNGKMHD 7929
++ Y DP I+V +L+ KI+ + +S+ K +PE+ L ++ I+ ILNLS + +++ +ER+ H + +WE +A+R SL +D + ++ WR EL+ W N + ++L V ++ I+ E++K N ES ++I++ I + TVG F+ LK + LS S R + +LLN Y ++ +L V E + ++ + + +++ I W T K H +FK ++ ++ QP + + + + K+VNL + IE D + K + S+ T+ R MW N +K ++ V + D +E ++ +K+L+ RK +++ K L+ GL + G S+L A++ P+ + ++ S ++ L L V + K E A+ +F N L+++ ++ S N + ++ F NE + +G N + +S CQ I IQ + I S + + RF LL++ L PS + + D + S+ Q KGIN+LIE + + + + E F + ++ +++ ++ T+D F +N + + +S+ +T T N+ ++F ++ + +E S K+L F K+ S L + V+K + + LP + + L +N H + + + T+ + GFC P+ P E K N E+ G+ GVG D+T+ + + +E N +D T NQ D+ DE + E+ D NG D
Sbjct: 3125 YNFYSDPNPSKAIEVRDLI---KIVESRAISLIK---NWPENFVLRGLKDAIDAILNLSPFSPIAEYLSKLERVFHLLSEWEKLASREYSLANEMDLIKKKIIDWRKFELSNWNNLLKLEEYKLSERVYPRLYSILQFIILKPFFENSKFTKQNLCESASIIVQFI----TDLTVGEFQLCLKCL-LS----FSQHAASLRICHGIDAMLLNIYHYFEQFLSKVSEAIHTQKQSLENSIKERILLMSWKDTNVYALKESAKKSHAELFKVLHRYREVLRQPVSSYLSQKHDWDSLLDTENNSAMWVAKKVNLSP--SYIEKMDTEIMKLVPVRFSNTPTTL----RLMWTLFANVEKPGSTFTNM--VSNLITDARELMKLTPETINDDNLSEIKHLKSRKHLLLTETFKTLKAFGLQYRVKAGIEENLSNLRNLLAVIPTFPVTSLSIEKVDRSLMKSLDFIPKFQTLAGHQHNDLSVPEVQKGVGLFNSMLSLQLGERAQLVEFTNELLAL-KNVYSEVGVNGSPLESFNNSSFNEVSSLGYDHDFENRAQAVSMLCQIYAIVIQKHSSISPTASFQSIGHELS------------------RFADLLSNKLFPSSIPLYASADKVSSIRDQ--------------------------QKGINDLIEYCRKK--------RTELPELSYCF-----KHLVSLQSLKSISRTQVDLTNDEFLNLMNFVLNLFDSLLSSIETA--TKNM-RTFKELAETSSFIEMSSCFSKVLRAFNLKFQSMKLSSLKEKLRSSSVDKMSCQLLMLFLPVCEQFINLAESVLDYFINVHNSNLDSLSKISTLFFMVANNGFCSPD-----------LPQEGKSNSGELESGTGLGSGVGAEDITNTLNEDDDLEELANEED----TANQSDL-DESEARELESDMNGVTKD 3966 HSP 7 Score: 82.8037 bits (203), Expect = 7.994e-14 Identity = 56/186 (30.11%), Postives = 95/186 (51.08%), Query Frame = 2
Query: 452 SKTTGSNVYRLFRALQVSKKPLLLEGSPGVGKTSLVTQLAKLVGFKVVRINLSEQTDANDLFGCFLPNENGKAGELSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPEINQKFMVEPGKTRLFACQNPVSEGSGRKGLPKSFVNRFTQVYMSSLSDSDYRNILNCLYPAIA 1009
+K+T N+ R+ L V K+P+LL G G+GK L+TQ+A +G +++RI+LS+ TD L G + + K GE W+ G L QA+ G WIL + A VL L L+ R ++ IP + + G ++FA + ++ G+ R Q+ + + +++ LYP ++
Sbjct: 135 TKSTQENLNRITPYL-VQKRPILLAGPEGIGKKFLITQIAAKLGQQIIRIHLSDSTDPKMLIGTYT---SPKPGEFEWQPGVLTQAVITGKWILFTNIEHAPSEVLSVLLPLLEKR-QLVIPSRGETIYAK-GSFQMFATSSMKTKILGQ---------RLWQILDLTYQPDECVEVVSTLYPVLS 305 HSP 8 Score: 81.6481 bits (200), Expect = 1.742e-13 Identity = 100/411 (24.33%), Postives = 172/411 (41.85%), Query Frame = 1
Query: 1156 ARTKEDQDACGKVYNELADKSRFARFIPSGLDFYITDQVLKIGQTQLSVNIANNTDNSKQFNKHLLLAGQLAILESMMLTVDMGWLTVLVGPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGS--FEQQKEGGKFKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGLVYGDRIEEIKPHKNFRLFLILDPDCGG----EISKAMRNRGLEICIIPENRELTLTDNDLLAILITIYKLSVKNADSLLRFHKTMILAQSETPQ-IKIG--------NINSLIQAAKLIKDGYSLDQSLRSVYGDIQMLFNVRDDVNRFINDCN--EEV--IDYQEIEQDDFSTCSSLFNIITEPKKSLFLRHVKALKKTLKTQQVVNKN----EKLIAC 2319
A ++ C NELA + A + + +D IG + + + +D++ + L +L ++ + + +L G G GKT+++ L+ TG+ L ++++ D DL GS + EGG+F W D+ + A+R G W++LD +N S SVL+ LN L+ + E V+ K H NFR+F +P G + ++ NR + + L + D++ I Y N D L+ K M Q + I G N+ ++ +L+ D + Y ++ +L +R +R + C +EV IDY E FS S F + SL +R V+ K L +Q ++ E +I C
Sbjct: 1481 ASLHAERQRCFAKLNELAGSNIMAS-KSADISIKFSDSSFFIGDFGIPLGDSVESDSTYSLHTDTTLMNASKVLRALQVLKPI----LLEGSPGVGKTSLITALARETGHQLVRINLSDQTDLMDLFGSDVPVEGGEGGQFAWRDAPFLAAMRNGHWVLLDELNLASQSVLEGLNACLDHRNEAYIPELDKVF-------KAHPNFRVFAAQNPQHQGGGRKGLPRSFINRFSVVYV------EALKEKDMIEIAACNYHQV--NEDWRLKIIKFMFRLQDNIEKDISFGSFGSPWEFNLRDTLRWLQLLNDAPKYTCVSPADYLEVMVLHRMRTVEDR-VRTCELFKEVFDIDY-EPRTIGFSLSSQCFKV----GHSLLVRDVERQKTLLDSQNILQSQLPVLESVITC 1865 HSP 9 Score: 80.8777 bits (198), Expect = 2.832e-13 Identity = 49/151 (32.45%), Postives = 80/151 (52.98%), Query Frame = 1
Query: 1426 GPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGSFEQQKEGGKFKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGLVYGDRIEEIKPHKNFRLFLILDPD--CGG--EISKAMRNRGLEICI--IPEN 1860
GP +GKT++++ ++ TG+ ++ + D + +G++ + G + + LV+ALR G W++LD +N VL+ LN LL+ N L + E ++ +KPH F LF +P G +S+A RNR LEI IPEN
Sbjct: 901 GPTSSGKTSMIEYVAKKTGHKFVRINNHEHTDLQEYIGTY-VTDDNGSLSFREGVLVEALRNGYWIVLDELNLAPTDVLEALNRLLDDNRELFIPETQVL-------VKPHPEFMLFATQNPPGVYAGRKHLSRAFRNRFLEIHFDDIPEN 1043 HSP 10 Score: 68.5514 bits (166), Expect = 1.327e-9 Identity = 48/128 (37.50%), Postives = 71/128 (55.47%), Query Frame = 1
Query: 1543 FEQQKEGGK----FKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGS-LLLTERGLVYGDRIEEIKPHKNFRLFLILDP--DCGG-EISKAMRNRGLEICIIPENRELTLTDNDLLAIL 1902
FE+ GG F +V+ LV+A+R G W++LD +N S L+ + LL S +LL+ERG I I PHKNFRLF ++P D G E+ + R+R EI + ++ L +DLL+I+
Sbjct: 612 FEKVLTGGSNGFMFSFVEGALVKAVRSGHWVLLDEINLASLETLEPIGQLLSSYESGILLSERG-----DITPITPHKNFRLFGCMNPSTDVGKRELEPSFRSRFTEIYVHSPDQNL----DDLLSII 730 HSP 11 Score: 67.0106 bits (162), Expect = 4.584e-9 Identity = 42/117 (35.90%), Postives = 66/117 (56.41%), Query Frame = 2
Query: 680 SWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACL-DHRGEIFIPEINQKFMVEPGKT-RLFACQNPVSEGSGRKGLPKSFVNRFTQVYMSSLSDSDYRNILNCLYPAIARETIG 1024
S+ +G L++A+++G W+LLDE+NLAS LE + L + I + E + P K RLF C NP S G++ L SF +RFT++Y+ S D + ++L+ + I IG
Sbjct: 626 SFVEGALVKAVRSGHWVLLDEINLASLETLEPIGQLLSSYESGILLSERGDITPITPHKNFRLFGCMNP-STDVGKRELEPSFRSRFTEIYVHS-PDQNLDDLLSIIQKYIGSLCIG 740 HSP 12 Score: 62.7734 bits (151), Expect = 9.273e-8 Identity = 36/98 (36.73%), Postives = 55/98 (56.12%), Query Frame = 1
Query: 1570 FKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGLVYGDRIEEIKPHKNFRLFLILDP--DCG-GEISKAMRNRGLEICIIP 1854
F+W D LV A++QGD+ +LD ++ SVL+RLN +LE + +L L E + + + F F ++P D G E+S A+RNR EI + P
Sbjct: 1296 FEWHDGALVTAMKQGDFFLLDEISLADDSVLERLNSVLELSRTLTLVE----HSNAAVSLTAKDGFAFFATMNPGGDYGKKELSPALRNRFTEIWVPP 1389 HSP 13 Score: 60.4622 bits (145), Expect = 3.884e-7 Identity = 35/98 (35.71%), Postives = 56/98 (57.14%), Query Frame = 2
Query: 683 WRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPEINQKFMVEPGKT--RLFACQNPVSEGSGRKGLPKSFVNRFTQVYMSSLSDSD 970
W DG L+ A+K GD+ LLDE++LA SVLE LN+ L+ + + E + + K FA NP + G+K L + NRFT++++ + D++
Sbjct: 1298 WHDGALVTAMKQGDFFLLDEISLADDSVLERLNSVLELSRTLTLVEHSNAAVSLTAKDGFAFFATMNPGGD-YGKKELSPALRNRFTEIWVPPMVDTE 1394 HSP 14 Score: 56.225 bits (134), Expect = 7.045e-6 Identity = 38/119 (31.93%), Postives = 63/119 (52.94%), Query Frame = 2
Query: 668 AGELSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFI----PEINQKFMVEPGKT-RLFACQNPVSEGSGRKGLPKSFVNRFTQVYM--SSLSDSDYRNILNCLYPA 1003
AG W DG LL+A++ G W +LD NL S +VL+ LN+ L+H+G + + E ++P RLF NPV L ++ NR ++++ +L++ D + ++ L PA
Sbjct: 2011 AGRFEWFDGYLLKAVEEGHWFVLDNANLCSPAVLDRLNSLLEHKGVLIVNEKTTEDGHPKTIKPHPNFRLFLTVNPV-----YGELSRAMRNRGVEIFLLKEALTEIDKKQ-MSLLEPA 2123
BLAST of Midasin vs. UniProt/SwissProt
Match: sp|Q869L3|MDN1_DICDI (Midasin OS=Dictyostelium discoideum OX=44689 GN=mdn1 PE=3 SV=2) HSP 1 Score: 303.523 bits (776), Expect = 1.391e-80 Identity = 164/405 (40.49%), Postives = 238/405 (58.77%), Query Frame = 2
Query: 38 VRFLLIVQKMSHEINFSVVTIRDLLSWVNFINISIGNKT-SLYQSIFHGAYMVIIDSINDKLNKSFYEELLIKKLDYLVKSSFPDIVECLDFQSSRFELSVK---------------------ENKVGIDPFYIELGSHSDWXXXXXXXXXXKNFQYSFNSKTTGSNVYRLFRALQVSKKPLLLEGSPGVGKTSLVTQLAKLVGFKVVRINLSEQTDANDLFGCFLPNENGKAGELSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPEINQKFMVEPGKTRLFACQNPVSEGSGRKGLPKSFVNRFTQVYMSSLSDSDYRNILNCLYPAIARETIGFIVEFVQNLREHVVVMKKFGTKGSPWEFNLRDLCRWCDLMVKHQHEPRKTRTPV 1186
+ +LL++QK N V+++RD+LSW++F+N+ + + S +S HGA +V++D N S E L + L++ +++ ++ Q + +L + EN+ GI PFYI + + Q+S ++ TT N R+ R +Q+ + +L+EGSPGVGKTSL+T +A G ++VRINLSEQTD DL G LP E G G+ WRDG L+AL+ G W+LLDE+NLASQ+VLE LN+CLDHR E++IPE+ + F P R+FACQNP+ +G GRKGLPKSF+NRFTQV++ L+ D I +YP I +TI ++EF + + ++ KF KG+PWEFNLRD RWCDL+VK +P P
Sbjct: 1689 IEYLLMIQK-----NKRVISLRDILSWISFMNLCMDKQLLSPNESYIHGACLVLLDGFGMGSNSSSESEGLKLREACLIR-----LIDQIESQDEKLKLQTELLSTSSSSSSLSSSIANIIKEENRFGIHPFYIPVRESN-----------VPKIQFSLSAPTTSKNAQRVLRGMQLPR-AILVEGSPGVGKTSLITAIANASGNQIVRINLSEQTDIMDLLGSDLPVEGGTGGQFEWRDGVFLEALRNGSWVLLDELNLASQTVLEGLNSCLDHRSEVYIPELGKTFACHPN-FRVFACQNPLHQGGGRKGLPKSFLNRFTQVFIDQLNQQDLLFISTAMYPTIPSKTIERMIEFNHQMFKESMIEHKFARKGAPWEFNLRDTFRWCDLIVK---DPTSISNPA 2067 HSP 2 Score: 199.134 bits (505), Expect = 4.770e-49 Identity = 102/280 (36.43%), Postives = 173/280 (61.79%), Query Frame = 1
Query: 9145 WSQYESKTEYLSRQLCEMLRIVLEPTKANKLGGAYRTGKRLNMKKVIPYIASGFRKDKIWLRRNKPSQREYQVIIGVDNSKSMAYNVNHQLFFESLSIVLNGLTFLEVGQVGLCKFGAKAEVVLPLYEKFSSEIGDRLIETFDFSDKCSKFIPILEQSIKLFEENQNFLSSSAVRXXXXXXXXXXXXXXXXXXXXPKFISLIREAHNKGIFVVVILLDHADSKDSILNLKMVIDDGCDFVR--FLDVFPLQNYTVLQDVTKLPTLLTDVLRQWFELSSSA 9978
W +YE T LS+ LCE LR++LEPT A KL G Y++GKR+NMKKVIPYIAS F+KDKIWLRR KP++R+YQV++ +D+++SMA + E+++++ ++ LEVGQ+G+ +FG ++V + FS + G +I F F + + L +++++ + N+ SSS + ++ L + + K+ +REA K +F+V I++D+ + +SIL+ + + + R ++ FP Y +L+ + +P++L+D LRQWF+L+ S
Sbjct: 5625 WKKYEQLTNDLSQDLCEQLRLILEPTLATKLQGDYKSGKRINMKKVIPYIASQFKKDKIWLRRTKPNKRQYQVLLAIDDTESMAAYHSGGFALEAMTMISRAMSRLEVGQLGIVRFGEDVQLVHSFDQVFSDQTGPEIITQFKFQQTKTNMVNFLSKTLQIMDMNKQ--SSSGEPAMQLVFIVSDGWSLRDPETTKKY---LREASIKNVFIVFIVIDNPVNNNSILDFESISFNNGKIQRTNYMSEFPFMYYVILRSLNNIPSILSDTLRQWFDLTKST 5899 HSP 3 Score: 131.339 bits (329), Expect = 1.682e-28 Identity = 108/363 (29.75%), Postives = 174/363 (47.93%), Query Frame = 2
Query: 83 FSVVTIRDLLSWVNFINISIGNKTSLYQSIFHGAYMVIIDSINDKLNKSFYE--ELLIKKLDYLVKSSFPDIVECLD-----FQSSRFELSVKENKVGIDPFYIELGSHSDWXXXXXXXXXXKNFQYSFNSKTTGSNVYRLFRALQVSKKPLLLEGSPGVGKTSLVTQLAKLVGFKVVRINLSEQTDANDLFGCFLPNENGKAGELSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPEINQKFMVEPGKTRLFACQNPVSEGSGRKGLPKSFVNRFTQVYMSSLSDSDYRNIL--NCLYPAIARETIGFIVEFVQNLRE--HVVVMKKFGTKGSPWEFNLRDLCRWCD 1138
FS+ T+ L++ + S G +++F G M + +LNKS Y E LIK+ Y+ K + L+ ++ + +E+ V I+ F+IE G+ + + + SN+ + R L K P+LL+G GKTS+V LA G + +RIN E TD + G ++ ++ GK L +++G L++A++ G W++LDE+NLA VLE LN LD E+FIPE + P LFA QNP GRK L ++F NRF ++++ + + + IL C P + + I++ +Q R+ + V K G RDL RW +
Sbjct: 1001 FSLRTLSRALNYTKHVTKSFG----FQRALFEGISMSFLT----QLNKSSYPMMEQLIKQ--YIKKGEVKLYNQPLNRPPPTSTTNNNNNNAEESYVKIEQFWIECGNEKPIIPT-----------HYILTPSIKSNLSNIARVLVSRKHPILLQGPTSSGKTSMVEYLAIRTGHRFIRINNHEHTDLQEYLGQYISDDKGK---LVFQEGILVEAVRKGYWVVLDELNLAPSEVLEALNRLLDDNRELFIPETQEVVRPHP-HFMLFATQNPPGLYGGRKVLSRAFRNRFLELHVDDIPEDELEEILCKRCALPPSYCKKLVLIMKELQLNRQGSNQVFAGKHGA------ITFRDLFRWAE 1332 HSP 4 Score: 124.405 bits (311), Expect = 2.113e-26 Identity = 88/243 (36.21%), Postives = 133/243 (54.73%), Query Frame = 1
Query: 1423 VGPAGTGKTTI--VKLLSDITGNHLKSMSMNSAMDTTD--------------LLGSFEQQKEGGKFKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGLVYGDRIEEIKPHKNFRLFLILDPDCGGEISKAMRNRGLEICIIPENRELTLT-----DNDLLAILITIYKLSVKNADSLLRFHKTMILAQSETPQIKIGNINSLIQAAKLIKD----GYSLDQSLRS 2076
+G G G TI + LLS I + L+ +S+ +D + S E++ G F+W+D L++AL G W+++D VNFC+ +VLDRLN LLE +G L+L ERG+V G +++ +KPH NFR+FL +D D GEIS+AMRNRG+EI + + L T DN L + I S+ +++ FH + S T + ++ L+ KLI D G+SL S+R+
Sbjct: 2297 IGSGGVGLITIEQLDLLSTIV-DALEKLSIQFNLDNNQEHLNRIQDVRQQIQRIKSIEKESVTGCFEWIDGLLIKALETGAWILIDNVNFCNPTVLDRLNPLLEQDGVLMLNERGMVDG-QVKVVKPHPNFRIFLTMD-DKKGEISRAMRNRGIEIYMAADQLSLMETPSNNFDNQQLLTALGIPLESL--TRTMIDFHNQIFTQLSSTIE-NPCTLSQLLYWGKLILDQLQRGFSLLNSIRN 2533 HSP 5 Score: 90.8929 bits (224), Expect = 2.556e-16 Identity = 57/178 (32.02%), Postives = 99/178 (55.62%), Query Frame = 2
Query: 509 KPLLLEGSPGVGKTSLVTQLAKLVGF-KVVRINLSEQTDANDLFGCFLPNENGKAGELSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPEINQKFMVEPGKTRLFACQNPVSEGSGRKGLPKSFVNRFTQVYMSSLSDSDYRNILNCLYPAIARETIGFIVEF 1039
KP+L+EG G GKT+++ +L+++ G ++RI+L +QTD+ L G ++ ++ GE W+ G L QA++ G WIL+++++LA VL L L++R +FIP + G +LFA Q S R + +T+V + +LS ++ + +L L+P + FI F
Sbjct: 296 KPILIEGVTGSGKTTMIEELSRITGNDNIIRIHLGDQTDSKVLLGTYITSDT--PGEFKWQAGALTQAVQEGRWILIEDIDLAPIEVLSVLIPLLENRT-LFIPGRGEVIEASNG-FQLFATQTLFGSHS-RDQNANILSHLWTRVVIEALSPTEMKQVLLTLFPQLTVMISKFIETF 468 HSP 6 Score: 86.6557 bits (213), Expect = 4.873e-15 Identity = 61/178 (34.27%), Postives = 96/178 (53.93%), Query Frame = 1
Query: 1570 FKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGLVYGDRIEEIKPHKNFRLFLILDP--DCG-GEISKAMRNRGLEICIIPENRELTLTDNDLLAIL---ITIYKLSVKNADSLLRFHKTMILAQSETPQIKIGNINSLIQAAKLIKDGYSLDQSLRSVYG 2085
F WVD LV+A++QGD+ ++D ++ +VL+RLN +LEP+ L+L E+G G IEE++ HK FR+ ++P D G E+S AMRNR EI + + DLL I+ T +L K + +L F + +++ Q I + +I S I L D L + ++G
Sbjct: 1564 FNWVDGPLVEAMKQGDYFLIDEISLAEDAVLERLNSVLEPSRLLVLAEKG---GVEIEELRGHKEFRILATMNPGGDFGKKELSPAMRNRFTEIWV-----PAISSHQDLLQIIEERFTSPQLKGKGS-LMLNFIEYLLMIQKNKRVISLRDILSWISFMNLCMDKQLLSPNESYIHG 1732 HSP 7 Score: 85.1149 bits (209), Expect = 1.711e-14 Identity = 64/245 (26.12%), Postives = 117/245 (47.76%), Query Frame = 1
Query: 1252 FYITDQVLKIGQTQLSVNIANNTDNSKQFNKHLLLAGQLAILESMMLTVDMGWLTVLVGPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGS--FEQQKEGGKFKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGLVYGDRIEEIKPHKNFRLFLILDP--DCGGE--ISKAMRNRGLEICIIPENRELTLTDNDLLAILITIYK-LSVKNADSLLRFHKTM 1965
FYI + + + Q S++ + N+++ +L M L + ++ G G GKT+++ +++ +GN + ++++ D DLLGS + GG+F+W D ++ALR G W++LD +N S +VL+ LN L+ + + E G + H NFR+F +P GG + K+ NR ++ I N++ DLL I +Y + K + ++ F+ M
Sbjct: 1810 FYIPVRESNVPKIQFSLSAPTTSKNAQR------------VLRGMQLPRAI----LVEGSPGVGKTSLITAIANASGNQIVRINLSEQTDIMDLLGSDLPVEGGTGGQFEWRDGVFLEALRNGSWVLLDELNLASQTVLEGLNSCLDHRSEVYIPELGKTFA-------CHPNFRVFACQNPLHQGGGRKGLPKSFLNRFTQVFIDQLNQQ------DLLFISTAMYPTIPSKTIERMIEFNHQM 2025 HSP 8 Score: 83.1889 bits (204), Expect = 5.390e-14 Identity = 52/154 (33.77%), Postives = 86/154 (55.84%), Query Frame = 1
Query: 1417 VLVGPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGSFEQQKEGGKFKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGLVYGDRIEEIKPHKNFRLFLILDPD--CGGE--ISKAMRNRGLEICI--IPEN 1860
+L GP +GKT++V+ L+ TG+ ++ + D + LG + + GK + + LV+A+R+G W++LD +N + VL+ LN LL+ N L + E E ++PH +F LF +P GG +S+A RNR LE+ + IPE+
Sbjct: 1132 LLQGPTSSGKTSMVEYLAIRTGHRFIRINNHEHTDLQEYLGQY-ISDDKGKLVFQEGILVEAVRKGYWVVLDELNLAPSEVLEALNRLLDDNRELFIPETQ-------EVVRPHPHFMLFATQNPPGLYGGRKVLSRAFRNRFLELHVDDIPED 1277 HSP 9 Score: 73.559 bits (179), Expect = 4.758e-11 Identity = 35/90 (38.89%), Postives = 58/90 (64.44%), Query Frame = 1
Query: 1282 GQTQLSVNIANNTDNSKQFNKHLLLAGQLAILESMMLTVDMGWLTVLVGPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGSFEQ 1551
G + ++ +N+ N + LL L LE++M ++M W+++L+GP T KT+ ++LL+ +TGN L SMNS++DTT++LG FEQ
Sbjct: 2142 GASSFDSSVLDNSSNIQ------LLQRLLNPLENIMKCIEMNWMSILIGPTSTSKTSSIRLLAQLTGNTLYEFSMNSSVDTTEILGGFEQ 2225 HSP 10 Score: 71.633 bits (174), Expect = 1.563e-10 Identity = 44/135 (32.59%), Postives = 74/135 (54.81%), Query Frame = 1
Query: 1375 LESMMLTVDMGWLTVLVGPAGTGKTTIVKLLSDITGN-HLKSMSMNSAMDTTDLLGSFEQQKEGGKFKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGLVYGDRIEEIKPHKNFRLF 1776
L S+ + V +G ++ G G+GKTT+++ LS ITGN ++ + + D+ LLG++ G+FKW L QA+++G W++++ ++ VL L LLE N +L + RG E I+ F+LF
Sbjct: 285 LNSLAIAVGLGKPILIEGVTGSGKTTMIEELSRITGNDNIIRIHLGDQTDSKVLLGTYITSDTPGEFKWQAGALTQAVQEGRWILIEDIDLAPIEVLSVLIPLLE-NRTLFIPGRG-------EVIEASNGFQLF 411 HSP 11 Score: 68.1662 bits (165), Expect = 1.772e-9 Identity = 54/159 (33.96%), Postives = 79/159 (49.69%), Query Frame = 2
Query: 680 SWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPEINQKFMVEPGKT----RLFACQNPVSEGSGRKGLPKSFVNRFTQVYMSSLSDSD-----YRNILNCLYPAIARETIGFIVEFVQNLREHVVVMKKFGTKGSPWEFNLRDLCR 1129
S+ +G L+ ++ G W+LLDEMNLA+ LE L+ D G I + E K VEP + ++FAC NP ++ G+K LP NRFT+ Y+ L + + +L+ L P I IVEF ++ G+ P F+LR L R
Sbjct: 859 SFVEGSLVNCIRKGYWVLLDEMNLATSETLESLSGLFDG-GSITLTE---KGEVEPVERHPNFKVFACMNPPTD-IGKKDLPPGIRNRFTEYYVDDLDNRGDLCLVVKTVLSNLVP---NPPIEDIVEFYLKTKQDAQNKMLDGSNQKPI-FSLRTLSR 1008 HSP 12 Score: 68.1662 bits (165), Expect = 1.772e-9 Identity = 56/246 (22.76%), Postives = 123/246 (50.00%), Query Frame = 1
Query: 5368 RPHDIYQDPIDVDNLLQAQKILPNANVSVTKLLGIYPEHTTXXXXXXXXXXXXXXSGDYSLIVFINNVERLIHEIQQWEVVAARHVSLIQHVDELNGLLVSWRHQELTMWENTISTANWQLERAVSREFFNHYNLIVQESNKIQYDNDFESINLILEPILNSLLNSTVGNFEYRLKTMKLSIESLISNREI-----DYRFKDRLKNILLNHYDFYRLYLGNVQEHLSSEYKRIGKELRDFIKTKRW 6090
+ ++IY+D ++ + ++ L + V +LL YPE L + K I+ ++ L + +E L+ + +WE A++ VS+ H++E++ L+V WR E+ W + + + E + +F Y+LI E + + +++ S N + L +S++G+F R++ +K + L S ++ + +K +L +I+ N + ++ ++ ++ L+ K I + +FI+ RW
Sbjct: 4025 KTYNIYRDS-NISEIGIIREPLISFRKRVFELLVEYPEQANLTLMVKLIDRLMTYPATDPLAKILTGLELLLRKSLEWESFASKAVSIQNHLNEISSLIVRWRKLEIESWPSIFQSQEKEAEVKALKSWFILYDLINDEPSSPELEDENLSKN--FHTLQQYLYSSSLGDFLTRIELLKSFYKQLNSTIKLIGTSKNTNYKQKLSDIIYNIFKYFENFISRFEDRLAKSIKPIEDKALEFIRLSRW 4267 HSP 13 Score: 66.6254 bits (161), Expect = 5.581e-9 Identity = 37/113 (32.74%), Postives = 64/113 (56.64%), Query Frame = 1
Query: 1519 DTTDLLGSFEQQKEGGKFKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGLVYGDRIEEIKPHKNFRLFLILDP--DCG-GEISKAMRNRGLEICI 1848
D L FE+ K F +V+ +LV +R+G W++LD +N ++ L+ L+GL + GS+ LTE+G +E ++ H NF++F ++P D G ++ +RNR E +
Sbjct: 841 DIKKLTIQFEKSKNNFAFSFVEGSLVNCIRKGYWVLLDEMNLATSETLESLSGLFD-GGSITLTEKG-----EVEPVERHPNFKVFACMNPPTDIGKKDLPPGIRNRFTEYYV 947 HSP 14 Score: 65.4698 bits (158), Expect = 1.226e-8 Identity = 45/165 (27.27%), Postives = 88/165 (53.33%), Query Frame = 2
Query: 680 SWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLD--------HRGEIFIPEI--NQKFMVEPGKTRLFACQNPVSEGSGRKGLPKSFVNRFTQVYMSSLSDSDYRNILNCLYPAIARETIGFIVEFVQNLREHVVVMKKFGTKGSPWEFNLRDLCRWCDLM 1144
+W DGPL++A+K GD+ L+DE++LA +VLE LN+ L+ +G + I E+ +++F R+ A NP + G+K L + NRFT++++ ++ S ++++L + + + N E++++++K + +LRD+ W M
Sbjct: 1565 NWVDGPLVEAMKQGDYFLIDEISLAEDAVLERLNSVLEPSRLLVLAEKGGVEIEELRGHKEF-------RILATMNPGGDF-GKKELSPAMRNRFTEIWVPAI--SSHQDLLQIIEERFTSPQLKGKGSLMLNFIEYLLMIQK-----NKRVISLRDILSWISFM 1714 HSP 15 Score: 62.003 bits (149), Expect = 1.550e-7 Identity = 56/187 (29.95%), Postives = 92/187 (49.20%), Query Frame = 1
Query: 7387 DKSFIKVNTHINALEQSLDTQKILDLFTKIKSFFCDL--NMNQVNKFRNLINEMVLPKLRSLEKVEKEFLGLLLNYHRVLISATDALLTVTTKLMKEGFCIPNDSAWNNEAENSNPSEKMNDFEMAGMDGGVGTNDVTDEIESESQIEGNNKD----KKDETENQPDIPDEDDGIEMTDDFNGKMHD 7929
D S K++ I+ QSL K+L+ F K+ + D +++ ++ +R +I +M P L + + + L+YH+ V +L +GFC P D + E S +D E GM G G DV+D++E + QIE N K ++ + + + DED+G +M DDF G+MHD
Sbjct: 4828 DGSISKLSEFIDHQVQSLRLSKLLEQFVKLHNIIVDGGGSVSTLSIYRQMIKQMG-PLLAQFMLIVNQSIVDSLSYHKTCCKLEYISTGVFIQLYSKGFCKPADGGDDGEGGEGGRSNFEDDVEGTGMGEGKGKKDVSDQLEDQGQIEDTNTQKKEEKDEDEDEEKEEKDEDEGFDMQDDFEGEMHD 5013
BLAST of Midasin vs. UniProt/SwissProt
Match: sp|Q12019|MDN1_YEAST (Midasin OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=MDN1 PE=1 SV=1) HSP 1 Score: 275.789 bits (704), Expect = 3.579e-72 Identity = 160/370 (43.24%), Postives = 220/370 (59.46%), Query Frame = 2
Query: 89 VVTIRDLLSWVNFIN---ISIGNKTSLYQSIFHGAYMVIIDSI----------NDKLNKSFYEELLIKKLDYLVKSSFPDIVECLDFQSSRFELSVKENKVGI--DPFYIELGSHSDWXXXXXXXXXXKNFQYSFNSKTTGSNVYRLFRALQVSKKPLLLEGSPGVGKTSLVTQLAKLVGFKVVRINLSEQTDANDLFGCFLPNENGKAGELSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPEINQKFMVEPGKTRLFACQNPVSEGSGRKGLPKSFVNRFTQVYMSSLSDSDYRNILNCLYPAIARETIGFIVEFVQNLREHVVVMKKFGTKGSPWEFNLRDLCRWCDLMVKH 1153
V+++RD+L+WV FIN I NK++ ++ GA MV ID++ N+ KS E +I+ L D +E +++ ++ E +VG+ P + + S S ++ + TT SN+ R+ RA+QV KP+LLEGSPGVGKTSL+T LA + G K+ RINLSEQTD DLFG P E ++GE W D P L+A+K G+W+LLDEMNLASQSVLE LNACLDHRGE +IPE++ F P +FA QNP +G GRKGLPKSFVNRF+ V++ L+ D I LYP+I + I +++ + L + V K +G GSPWEFNLRD RW L+ ++
Sbjct: 1611 VISLRDILAWVEFINKVFPKIQNKST---ALIQGASMVFIDALGTNNTAYLAENENDLKSLRTECIIQLLKLC-----GDDLELQQIETNEIIVTQDELQVGMFKIPRFPDAQSSS----------------FNLTAPTTASNLVRVVRAMQV-HKPILLEGSPGVGKTSLITALANITGNKLTRINLSEQTDLVDLFGADAPGE--RSGEFLWHDAPFLRAMKKGEWVLLDEMNLASQSVLEGLNACLDHRGEAYIPELDISFSCHPN-FLVFAAQNPQYQGGGRKGLPKSFVNRFSVVFIDMLTSDDLLLIAKHLYPSIEPDIIAKMIKLMSTLEDQVCKRKLWGNSGSPWEFNLRDTLRWLKLLNQY 1952 HSP 2 Score: 187.963 bits (476), Expect = 1.193e-45 Identity = 109/288 (37.85%), Postives = 169/288 (58.68%), Query Frame = 1
Query: 9136 RFTWSQYESKTEYLSRQLCEMLRIVLEPTKANKLGGAYRTGKRLNMKKVIPYIASGFRKDKIWLRRNKPSQREYQVIIGVDNSKSMAYNVNHQLFFESLSIVLNGLTFLEVGQVGLCKFGAKAEVVLPLYEKFSSEIGDRLIETFDFSDKCSKFIPILEQSIKLFEENQNFLSSSAVRXXXXXXXXXXXXXXXXXXXXPKFISLIREAHNKGIFVVVILLDHADSKDSILNLKMV--IDD-----GCDFVRFLDVFPLQNYTVLQDVTKLPTLLTDVLRQWF-ELSSS 9975
R W + E T L +L E LR++LEPT A KL G Y+TGKRLNMK++IPYIAS FRKDKIWLRR KPS+R+YQ++I +D+SKSM+ + +L F+SL +V LT LE G + + KFG + V ++FS+E G R + F F + + ++ +S K+FE + + + + + + + + L+R A I +V +++D S +SIL++ V I D ++LD FP + Y V+ D+++LP +L+ +LRQ+F +L+SS
Sbjct: 4630 RELWHKSEISTADLVSRLGEQLRLILEPTLATKLKGDYKTGKRLNMKRIIPYIASQFRKDKIWLRRTKPSKRQYQIMIALDDSKSMSESKCVKLAFDSLCLVSKTLTQLEAGGLSIVKFGENIKEVHSFDQQFSNESGARAFQWFGFQETKTDVKKLVAESTKIFERARAMVHNDQWQLEIVISDGICEDHETIQ-------KLVRRARENKIMLVFVIIDGITSNESILDMSQVNYIPDQYGNPQLKITKYLDTFPFEFYVVVHDISELPEMLSLILRQYFTDLASS 4910 HSP 3 Score: 133.65 bits (335), Expect = 3.489e-29 Identity = 85/225 (37.78%), Postives = 121/225 (53.78%), Query Frame = 2
Query: 470 NVYRLFRALQVSKKPLLLEGSPGVGKTSLVTQLAKLVGFKVVRINLSEQTDANDLFGCFLPNENGKAGELSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPEINQKFMVEPGKTRLFACQNPVSEGSGRKGLPKSFVNRFTQVYMSSLSDSDYRNIL--NC-LYPAIARETIGFIVEFVQNLREHVVVMKKFGTKGSPWEFNLRDLCRWC 1135
N+ L RA + P+L++G GKTS++ LA + G K VRIN E TD + G ++ ++ GK LS+++G L++AL+ G WI+LDE+NLA VLE LN LD E+FIPE + P LFA QNP GRK L ++F NRF +++ + + IL C + P+ A++ IVE + L + F K S LRDL RW
Sbjct: 1064 NMMNLVRATSGKRFPVLIQGPTSSGKTSMIKYLADITGHKFVRINNHEHTDLQEYLGTYVTDDTGK---LSFKEGVLVEALRKGYWIVLDELNLAPTDVLEALNRLLDDNRELFIPETQEVVHPHPD-FLLFATQNPPGIYGGRKILSRAFRNRFLELHFDDIPQDELEIILRERCQIAPSYAKK----IVEVYRQLSIERSASRLFEQKNSF--ATLRDLFRWA 1278 HSP 4 Score: 110.923 bits (276), Expect = 2.063e-22 Identity = 62/121 (51.24%), Postives = 79/121 (65.29%), Query Frame = 1
Query: 1489 LKSMSMNSAMDTTDLLGSFEQQKEGGKFKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGLVYGD-RIEEIKPHKNFRLFLILDPDCGGEISKAMRNRGLEICI 1848
LK+MSMN + + ++ KF+W D LV+A+ +G WLILD N CS SVLDRLN LLE +GSLL+ E G R+ +KPH NFRLFL +DP GE+S+AMRNRG+EI I
Sbjct: 2170 LKTMSMN-----IEKMTEIITKEASVKFEWFDGMLVKAVEKGHWLILDNANLCSPSVLDRLNSLLEIDGSLLINECSQEDGQPRV--LKPHPNFRLFLTMDPKY-GELSRAMRNRGVEIYI 2282 HSP 5 Score: 87.8113 bits (216), Expect = 2.080e-15 Identity = 53/155 (34.19%), Postives = 86/155 (55.48%), Query Frame = 1
Query: 1417 VLVGPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGSFEQQKEGGKFKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGLVYGDRIEEIKPHKNFRLFLILDPD--CGGE--ISKAMRNRGLEICI--IPENR 1863
++ GP +GKT+++K L+DITG+ ++ + D + LG++ + GK + + LV+ALR+G W++LD +N VL+ LN LL+ N L + E E + PH +F LF +P GG +S+A RNR LE+ IP++
Sbjct: 1080 LIQGPTSSGKTSMIKYLADITGHKFVRINNHEHTDLQEYLGTY-VTDDTGKLSFKEGVLVEALRKGYWIVLDELNLAPTDVLEALNRLLDDNRELFIPETQ-------EVVHPHPDFLLFATQNPPGIYGGRKILSRAFRNRFLELHFDDIPQDE 1226 HSP 6 Score: 84.3445 bits (207), Expect = 2.264e-14 Identity = 52/170 (30.59%), Postives = 87/170 (51.18%), Query Frame = 1
Query: 1417 VLVGPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGSFEQQKEGGKFKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGLVYGDRIEEIKPHKNFRLFLILDPDCGG----EISKAMRNRGLEICIIPENRELTLTDNDLLAILITIY 1914
+L G G GKT+++ L++ITGN L ++++ D DL G+ + G+F W D+ ++A+++G+W++LD +N S SVL+ LN L+ G + E + + H NF +F +P G + K+ NR + I LT +DLL I +Y
Sbjct: 1744 LLEGSPGVGKTSLITALANITGNKLTRINLSEQTDLVDLFGADAPGERSGEFLWHDAPFLRAMKKGEWVLLDEMNLASQSVLEGLNACLDHRGEAYIPELDISFS-------CHPNFLVFAAQNPQYQGGGRKGLPKSFVNR-FSVVFID-----MLTSDDLLLIAKHLY 1900 HSP 7 Score: 76.2554 bits (186), Expect = 8.035e-12 Identity = 65/273 (23.81%), Postives = 116/273 (42.49%), Query Frame = 2
Query: 503 SKKPLLLEGSPGVGKTSLVTQLAKLVGFKVVRINLSEQTDAND--------------------------------------LFGCFLPNENGKAGE----------------LSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPE--INQKFMVEPGKTRLFACQNPVSEGSGRKGLPKSFVNRFTQVYMSSLSD-SDYRNILNCLYPAIARETIGFIVEFVQNLREHVVVMKKFGTKGSPWEFNLRDLCRWCDLMVK 1150
+K+P+LL G G GKT++ LA+ +G +++ +N + T+ D L + ++N E W DGPL+QA++ G++ LLDE++LA SVLE LN+ L+ + + E + + + FA NP + G+K L + NRFT++++ S+ D +D I++ ++ IV+F + + K G + +LRD+ W + + K
Sbjct: 1360 NKEPVLLVGETGCGKTTICQLLAQFMGRELITLNAHQNTETGDILGAQRPVRNRSEIQYKLIKSLKTALNIANDQDVDLKELLQLYSKSDNKNIAEDVQLEIQKLRDSLNVLFEWSDGPLIQAMRTGNFFLLDEISLADDSVLERLNSVLEPERSLLLAEQGSSDSLVTASENFQFFATMNPGGD-YGKKELSPALRNRFTEIWVPSMEDFNDVNMIVSSRLLEDLKDLANPIVKFSEWFGK-----KLGGGNATSGVISLRDILAWVEFINK 1626 HSP 8 Score: 75.485 bits (184), Expect = 1.339e-11 Identity = 47/121 (38.84%), Postives = 72/121 (59.50%), Query Frame = 2
Query: 461 TGSNVYRLFRALQVSKKPLLLEGSPGVGKTSLVTQLAKLVGFK--VVRINLSEQTDANDLFGCFLPNENGKAGELSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIP 817
T S++ +L R +Q S P++L G G GKT L+ +L+K +G +V+I+L EQTDA L G + + K G WR G L A+K G W+L+++++ A VL L + L+ R E+ IP
Sbjct: 294 TVSSLRQLGRKIQNST-PIMLIGKAGSGKTFLINELSKYMGCHDSIVKIHLGEQTDAKLLIGTYTSGD--KPGTFEWRAGVLATAVKEGRWVLIEDIDKAPTDVLSILLSLLEKR-ELTIP 410 HSP 9 Score: 72.7886 bits (177), Expect = 7.212e-11 Identity = 40/96 (41.67%), Postives = 60/96 (62.50%), Query Frame = 1
Query: 1570 FKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGLVYGDRIEEIKPHKNFRLFLILDP--DCG-GEISKAMRNRGLEICI 1848
F+W D L+QA+R G++ +LD ++ SVL+RLN +LEP SLLL E+G D + + +NF+ F ++P D G E+S A+RNR EI +
Sbjct: 1472 FEWSDGPLIQAMRTGNFFLLDEISLADDSVLERLNSVLEPERSLLLAEQG--SSDSL--VTASENFQFFATMNPGGDYGKKELSPALRNRFTEIWV 1563 HSP 10 Score: 72.7886 bits (177), Expect = 8.386e-11 Identity = 50/148 (33.78%), Postives = 83/148 (56.08%), Query Frame = 1
Query: 1486 HLKSMSMNSAMDTTDLLGSFEQQKEGGK----FKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLL-EPNG-SLLLTERGLVYGDRIEEIKPHKNFRLFLILDP--DCGG-EISKAMRNRGLEICIIPENRELTLTDNDLLAIL 1902
H K + ++ D D + FE Q + F +V+ +LV+ +R G+WL+LD VN +A L+ ++ LL EP+ S+LL+E+G E IK H +FR+F ++P D G ++ +R+R EI + R++T DLL+I+
Sbjct: 777 HEKKLLLDKWADFNDSVKKFEAQSSSIENSFVFNFVEGSLVKTIRAGEWLLLDEVNLATADTLESISDLLTEPDSRSILLSEKG-----DAEPIKAHPDFRIFACMNPATDVGKRDLPMGIRSRFTEIYVHSPERDIT----DLLSII 915 HSP 11 Score: 64.6994 bits (156), Expect = 2.093e-8 Identity = 38/119 (31.93%), Postives = 70/119 (58.82%), Query Frame = 2
Query: 689 DGPLLQALKAGDWILLDEMNLASQSVLECLNACL---DHRGEIFIPEINQKFMVEPGKT----RLFACQNPVSEGSGRKGLPKSFVNRFTQVYMSSLSDSDYRNILNCLYPAIARETIG 1024
+G L++ ++AG+W+LLDE+NLA+ LE ++ L D R + +++K EP K R+FAC NP ++ G++ LP +RFT++Y+ S + D ++L+ + I + ++
Sbjct: 813 EGSLVKTIRAGEWLLLDEVNLATADTLESISDLLTEPDSRSIL----LSEKGDAEPIKAHPDFRIFACMNPATD-VGKRDLPMGIRSRFTEIYVHS-PERDITDLLSIIDKYIGKYSVS 925 HSP 12 Score: 62.7734 bits (151), Expect = 7.730e-8 Identity = 71/275 (25.82%), Postives = 119/275 (43.27%), Query Frame = 1
Query: 5377 DIYQDPIDVDNLLQAQKILPNANVSVTKLLGIYPEHTTXXXXXXXXXXXXXXSGDYSLIVFINNVERLIHEIQQWEVVAARHVSLIQHVDELNGLLVSWRHQELTMWENTISTANWQLERAVSREFFNHYNLIVQESNKIQYDNDFESINLILEPILNSLLN-STVGNFEYRLKTMKLSIE--SLISNREIDYRFKDRLKNILLNHYDFYRLYLGNVQEHLSSEYKRIGKELRDFIKTKRWGKTPPKITDVL-----KCHKIVFKYKRQWSGIIN 6177
D Y D + +A I+ SV KLL +PEH TL + + LN L + +E++ + +WE A+ VSL V + L+VSWR EL W+ ++ + + +++ + +F Y IV SN + + +L LN + ST+G F RL +K + LI R ++ +L N FY + + E +++ K + KE+ D I W D L K H ++K R++ ++N
Sbjct: 3281 DFYHD-FSIPEFQKAGDIIETVLKSVLKLLKQWPEHATLKELYRVSQEFLNYPIKTPLARQLQKIEQIYTYLAEWEKYASSEVSLNNTVKLITDLIVSWRKLELRTWKGLFNSEDAKTRKSIGKWWFYLYESIVI-SNFVSEKKETAPNATLLVSSLNLFFSKSTLGEFNARLDLVKAFYKHIQLIGLR------SSKIAGLLHNTIKFYYQFKPLIDERITNGKKSLEKEIDDIILLASWKDVN---VDALKQSSRKSHNNLYKIVRKYRDLLN 3544 HSP 13 Score: 61.2326 bits (147), Expect = 2.518e-7 Identity = 28/86 (32.56%), Postives = 49/86 (56.98%), Query Frame = 1
Query: 1354 LAGQLAILESMMLTVDMGWLTVLVGPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGSFEQQKEGGKFKWVDSTLVQALRQ 1611
L +A+ ES++ ++ W VLVGP+ +GKT ++ L+ I G + SMNS +D+ D+LG +EQ + ++ L +R+
Sbjct: 2030 LECNVAVYESVLKAINNNWPLVLVGPSNSGKTETIRFLASILGPRVDVFSMNSDIDSMDILGGYEQVDLTRQISYITEELTNIVRE 2115
BLAST of Midasin vs. TrEMBL
Match: A0A4S2LK77 (Midasin OS=Opisthorchis felineus OX=147828 GN=CRM22_006983 PE=3 SV=1) HSP 1 Score: 337.806 bits (865), Expect = 6.869e-138 Identity = 181/388 (46.65%), Postives = 239/388 (61.60%), Query Frame = 2
Query: 86 SVVTIRDLLSWVNFINISIGNKTS--------------LYQSIFHGAYMVIIDSINDKLNKSFYEELLIKKLDYLVKSSFPDI-------VECLDFQSSR------------FELSVKENKVGIDPFYIELGSHSDWXXXXXXXXXXKNFQYSFNSKTTGSNVYRLFRALQVSKKPLLLEGSPGVGKTSLVTQLAKLVGFKVVRINLSEQTDANDLFGCFLPNENGKAGELSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPEINQKFMVEPGKTRLFACQNPVSEGSGRKGLPKSFVNRFTQVYMSSLSDSDYRNILNCLYPAIARETIGFIVEFVQNLREHVVVMKKFGTKGSPWEFNLRDLCRWCDLMVK 1150
S TIRDLL+W+ F+ S+ +++S L + HGA ++ +D+++ K L + ++ I VE L F +++ F L+ G DPFYI++G SD +L N +++F++ T N+ RL RALQV K+ LLLEGSPGVGKTSLV LAK G +VVRINLSE T+A+DL GC LP E G +WRDGPLLQ L+ G WI+LDEMNLASQSVLECLNACLDHRGE++IPE+ F ++PG TRLFACQNPV EG GRKGLPKSF+NRFTQV++ L+ +D I+ LYP + + +V F L + V V ++FGT+G PWEFNLRDL RW +L++K
Sbjct: 1452 SPPTIRDLLAWIQFME-SVCSRSSNPAATPPRLSSSIDLLSACLHGACLIFLDAVDCKPTGILEGPGLAAQGVKFLRERLESIASEHGLPVETL-FIAAKYTCAGDGSVDGGFGLTSDGRMFGADPFYIDMGPSSDLSLAL-------NSRFNFSASTPAVNLRRLLRALQVPKRALLLEGSPGVGKTSLVLALAKATGHEVVRINLSEATEASDLLGCDLPVEGAAPGVFAWRDGPLLQGLREGRWIVLDEMNLASQSVLECLNACLDHRGELYIPELGATFKIQPGATRLFACQNPVREGGGRKGLPKSFLNRFTQVFLQPLTRADQEFIMQQLYPRLPAPAVQLMVHFNWKLADEVNVAREFGTQGGPWEFNLRDLMRWAELILK 1830 HSP 2 Score: 187.578 bits (475), Expect = 6.869e-138 Identity = 107/269 (39.78%), Postives = 164/269 (60.97%), Query Frame = 1
Query: 1159 RTKEDQDACGKVYNELADK---SRFARFIPSGLDFYITDQVLKIGQTQLSVNIANNTDNSKQFNKHLLLAGQLAILESMMLTVDMGWLTVLVGPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGSFEQQKEGGKFKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGLVYGDRIEEIKPHKNFRLFLILDPDCGGE------ISKAMRNRGLEICIIPENRELTLTDNDLLAILITI-YKLSVKNA 1935
RT+ D+ +++N + + + + P G I + +L+IG + A D SK N L L Q + LES++ ++MGW+ +LVGP G GK T++ + + + G+ L +M M+ DT +LLGS EQ + GG F W+DS LV+ +R G W+++++ CS SVLDRLN +LEP+G L+L+ERGL R+ +KPH NFRL LI+D + GG +S+AMRNRG+EI + ++L++T+ DL IL +I + SV NA
Sbjct: 1852 RTEADKHKVAELWNSVCRERGATNMVYYKPLG-RISIVNNILRIGVA--GFDTALMVDPSKLPNL-LFLDHQRSQLESLLKILEMGWMAILVGPPGCGKRTLINICATLFGHPLSTMHMSPTADTVELLGSLEQHEAGGHFCWIDSPLVKGMRHGHWVVIESAQLCSPSVLDRLNSVLEPDGELILSERGLDSSGRLIRLKPHPNFRLILIVD-EVGGAGAGSTGVSRAMRNRGVEIAVY---KDLSVTNLDLDRILTSIGTRNSVVNA 2112 HSP 3 Score: 227.254 bits (578), Expect = 4.763e-55 Identity = 129/279 (46.24%), Postives = 186/279 (66.67%), Query Frame = 1
Query: 9139 FTWSQYESKTEYLSRQLCEMLRIVLEPTKANKLGGAYRTGKRLNMKKVIPYIASGFRKDKIWLRRNKPSQREYQVIIGVDNSKSMAYNVNHQLFFESLSIVLNGLTFLEVGQVGLCKFGAKAEVVLPLYEKFSSEIGDRLIETFDFSDKCSKFIPILEQSIKLFEENQNFLSSSAVRXXXXXXXXXXXXXXXXXXXXPKFISLIREAHNKGIFVVVILLDHADSKDSILNLKMVIDDGCDFVRFLDVFPLQNYTVLQDVTKLPTLLTDVLRQWFELSSS 9975
F WS +++ L+RQLCE LR+VLEPTKA ++ G YRTGKR+NM+K+IPY+AS FRKDKIWLRR++PSQREY+++I VD+S SM+ N+ Q+ F++L+ V+ L LEVG++G+C FG ++ L + + + G ++ F F + I +L+ +IK + NF +++ SQLLL+LSDG SED DPK + +R A + +F V ++LD K SI +L+ +G V ++D FPL Y VL+DVT LP LL+D LRQWFEL +S
Sbjct: 5070 FEWSACSARSAGLARQLCESLRLVLEPTKAARMRGDYRTGKRINMRKIIPYLASHFRKDKIWLRRSQPSQREYRILIAVDDSSSMSDNLCRQMTFDALATVVTALNLLEVGRIGVCSFGESVRILHDLQDTWVGDAGANVLAQFTFKQSRTSLIQLLQNAIKAMQMC-NFPTTTG-SMPSQLLLILSDGVFSEDPQDPKVQAAVRLARDSRLFPVCLILDDVRKKHSIFDLRRYTSNG-KLVPYMDTFPLPFYLVLRDVTALPQLLSDALRQWFELEAS 5345 HSP 4 Score: 113.62 bits (283), Expect = 1.045e-20 Identity = 76/223 (34.08%), Postives = 111/223 (49.78%), Query Frame = 2
Query: 512 PLLLEGSPGVGKTSLVTQLAKLVGFKVVRINLSEQTDANDLFGCFLPN------------ENGKAGELSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPEINQKFMVEPGKTRLFACQNPVSEGSGRKGLPKSFVNRFTQVYMSSLSDSDYRNILN--CLYPAIARETIGFIVEFVQNLREHVVVMKKFGTKGSPWEFNLRDLCRWCD 1138
P+LL+G VGKTSL+T LA+ +G RIN E T+ G + ++G L ++ GPL+QA++ G WI+LDE+NLA +LE LN LD +FIP+ + P + RLFA QNP +GRK L ++ NRF +++ + + IL C A+ + +VE + L+ F K S LRDL RW +
Sbjct: 877 PILLQGETSVGKTSLITYLAERIGQVCYRINNHEHTEQQTYLGTYTVASATAQANVTDQVKDGTPPPLVFQHGPLVQAMRLGHWIILDELNLAPTEILEALNRVLDDNRTLFIPDTQEVIRAHP-QFRLFATQNPPGLYAGRKVLSRALRNRFIELHFDPIPHGELEVILEKRC---ALPQSRAHRLVEVMHRLQLARCQSNVFLGKDS--FITLRDLFRWAE 1093 HSP 5 Score: 90.1225 bits (222), Expect = 1.507e-13 Identity = 67/280 (23.93%), Postives = 128/280 (45.71%), Query Frame = 1
Query: 5377 DIYQDPIDVDNLLQAQKILPNANVSVTKLLGIYPEHTTXXXXXXXXXXXXXXSGDYSLIVFINNVERLIHEIQQWEVVAARHVSLIQHVDELNGLLVSWRHQELTMWENTISTANWQLERAVSREFFNHYNLIVQESNKIQYDNDF---ESINLILEPILNSLLNSTVGNFEYRLKTMKLSIESLISNREIDYRFKDRLKNILLNHYDFYRLYLGNVQEHLSSEYKRIGKELRDFIKTKRWGKTP---PKITDVLKCHKIVFKYKRQWSGIINQPCTKFF 6198
D+Y+DPI +D + +L + V ++L +P +L+++ I + L+ F+ VE + E+ +WE A+RHVSL++ + E+ LL+ WR EL W +T+ TA + +F+ +++ ++ D+ +S + E ++ + N +G F R + + +L + + + K I+ N FY + VQ + K I KELR + +WG ++V +C + + K R W ++ +P F
Sbjct: 3439 DVYRDPIPLDTASKLLTVLEHLERRVRQILHEWPGQPSLVHLITLIRRLEGFHVSDPLMKFLTGVEMIWQEMHEWEKNASRHVSLMEQIKEVCELLIEWRKMELKCWLSTLDTATSAFSNRAATLWFHLHDVFLRPPGHATSDSTVGKTQSESDRCEVLVELMENGPLGEFPMRWRLLAALRTALDIWPGLPTQDCETNKRIVGNVVWFYGQFFSTVQNEIKRLRKPIEKELRGVVHITKWGDYACFWSVKSNVDRCKRTIHKQVRNWETVLRRPVRPLF 3718 HSP 6 Score: 80.1073 bits (196), Expect = 1.310e-10 Identity = 54/156 (34.62%), Postives = 85/156 (54.49%), Query Frame = 1
Query: 1417 VLVGPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGSFEQQKEG---GKFKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGLVYGDRIEEIKPHKNFRLFLILDP--DCGGE--ISKAMRNRGLEICIIPENR 1863
+L G G GKT++V L+ TG+ + ++++ A + +DLLG + EG G F W D L+Q LR+G W++LD +N S SVL+ LN L+ G L + E G + +I+P RLF +P + GG + K+ NR ++ + P R
Sbjct: 1620 LLEGSPGVGKTSLVLALAKATGHEVVRINLSEATEASDLLGC-DLPVEGAAPGVFAWRDGPLLQGLREGRWIVLDEMNLASQSVLECLNACLDHRGELYIPELGATF-----KIQPGAT-RLFACQNPVREGGGRKGLPKSFLNRFTQVFLQPLTR 1768 HSP 7 Score: 77.7962 bits (190), Expect = 6.540e-10 Identity = 65/212 (30.66%), Postives = 97/212 (45.75%), Query Frame = 1
Query: 1327 SKQFNKHLLLAGQL--AILESMMLTVDMGWLTVLVGPA-------------GTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGSFEQQK-----------EGGKFKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGLVYGD----RIEEIKPHKNFRLFLILDPDCGG-----EISKAMRNRGLEI-CIIP 1854
SK+F + LL + A E ++ T DM + VL+G A GKTT+ +L + + L ++ + + D LG + +G F+WVD LV A+ +G+ +LD ++ +VL+RLN LLEP L L ER G ++ H FRL ++P GG E+S A+RNR EI C P
Sbjct: 1171 SKEFLQPLLSESDVRPAGFEHVVWTRDMRRMLVLIGNALKYKEPILLVGETSCGKTTVCQLFAALKHQKLHCVNCHQYTEAGDFLGGLRPIRRVSSQDGAEVDDGRLFEWVDGPLVVAMSRGEMFLLDEISLADDAVLERLNSLLEPERRLNLAERSEGAGSIGVLETNDLTAHIGFRLVATMNP--GGDYGKKELSPALRNRFTEIWCPTP 1380 HSP 8 Score: 77.411 bits (189), Expect = 1.019e-9 Identity = 54/188 (28.72%), Postives = 89/188 (47.34%), Query Frame = 2
Query: 506 KKPLLLEGSPGVGKTSLVTQLAKLVGFKVVRINLSEQTDANDLFGCFLPNENGKAGELSWRDGPLLQALKAGDWILLDEMNLAS---QSVLECL---NACLDHRGEIFIPE--------------INQKFMVEPGKTRLFACQNPVSEGSGRKGLPKSFVNRFTQVYM-SSLSDSDYRNILNCLYPAI 1006
+KP+LL G G GKT + +A+L V R+ +SEQTD+ L G + E G+ WR GPLL +L G W++L++++ Q +L L N HR ++ P + ++ ++ C NP +E FV+R + M +LSD + R +++ LYP++
Sbjct: 82 RKPVLLAGPVGCGKTRCIDFVARLQKSSVCRVQISEQTDSKSLLGAYCCTET--PGQFVWRPGPLLTSLTQGSWLVLEDVDRGPADLQMLLAPLFKPNHSSCHRLDLIDPTTGLPVDQHRDFRILMTRRLVLSRTNEWRMECDNPCAE----------FVDRHCSIIMLPALSDDNLRQLVSRLYPSL 257 HSP 9 Score: 68.9366 bits (167), Expect = 3.241e-7 Identity = 56/168 (33.33%), Postives = 81/168 (48.21%), Query Frame = 2
Query: 689 DGPLLQALKAGDWILLDEMNLASQSVLECLNACLD-HRGEIFIPEINQKFMVEPGKT-RLFACQNPVSEGSGRKGLPKSFVNRFTQVYMSSL----SDSDY---------------RNILNCLYPAIARETIGFIVEFVQNLREHVVVMKKFGTKGSPWEFNLRDLCR 1129
+G L++A++ GDW+LLDE+NLA +L+CL+ LD RG + + E K ++ LFA NP S G++ LP NRFT++Y+ L +DS R L L P+ + I +V LR+ V G P F+LR LCR
Sbjct: 575 EGALVRAVQDGDWVLLDEINLAPAEMLDCLSGLLDSDRGSVTLVERGDKTPLKRNPDFHLFAAMNP-STDVGKRELPVGLRNRFTEIYVPELDPGVNDSQLNPIRVTDREDLAILIRAYLLPLNPSPTQ--ITTVVRLYAALRQAAVEGLVDGVGQKP-HFSLRTLCR 738 HSP 10 Score: 68.1662 bits (165), Expect = 6.335e-7 Identity = 43/149 (28.86%), Postives = 75/149 (50.34%), Query Frame = 2
Query: 518 LLEGSPGVGKTSLVTQLAKLVGFKVVRINLSEQTDANDLFGCFLPNENGKAGELSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPE-----INQKFMVEPGKT-RLFACQNPV-SEGSGRKGLPKSFVNRFTQV 943
+L G PG GK +L+ A L G + +++S D +L G +E G G W D PL++ ++ G W++++ L S SVL+ LN+ L+ GE+ + E + ++P RL + V G+G G+ ++ NR ++
Sbjct: 1937 ILVGPPGCGKRTLINICATLFGHPLSTMHMSPTADTVELLGSLEQHEAG--GHFCWIDSPLVKGMRHGHWVVIESAQLCSPSVLDRLNSVLEPDGELILSERGLDSSGRLIRLKPHPNFRLILIVDEVGGAGAGSTGVSRAMRNRGVEI 2083 HSP 11 Score: 67.781 bits (164), Expect = 7.365e-7 Identity = 55/168 (32.74%), Postives = 82/168 (48.81%), Query Frame = 2
Query: 506 KKPLLLEGSPGVGKTSLVTQLAKLVGFKVVRINLSEQTDANDLFGCFLP-----NENGKA---GEL-SWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHR------------GEIFIPEINQKFMVEPGKTRLFACQNPVSEGSGRKGLPKSFVNRFTQVY 946
K+P+LL G GKT++ A L K+ +N + T+A D G P +++G G L W DGPL+ A+ G+ LLDE++LA +VLE LN+ L+ G I + E N + RL A NP + G+K L + NRFT+++
Sbjct: 1212 KEPILLVGETSCGKTTVCQLFAALKHQKLHCVNCHQYTEAGDFLGGLRPIRRVSSQDGAEVDDGRLFEWVDGPLVVAMSRGEMFLLDEISLADDAVLERLNSLLEPERRLNLAERSEGAGSIGVLETND--LTAHIGFRLVATMNPGGD-YGKKELSPALRNRFTEIW 1376 HSP 12 Score: 65.4698 bits (158), Expect = 4.169e-6 Identity = 48/132 (36.36%), Postives = 75/132 (56.82%), Query Frame = 1
Query: 1570 FKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPN-GSLLLTERGLVYGDRIEEIKPHKNFRLFLILDP--DCGG-EISKAMRNRGLEICIIPE---------NRELTLTDNDLLAILITIYKLSV 1926
F +++ LV+A++ GDW++LD +N A +LD L+GLL+ + GS+ L ER GD+ +K + +F LF ++P D G E+ +RNR EI +PE + +TD + LAILI Y L +
Sbjct: 571 FAFIEGALVRAVQDGDWVLLDEINLAPAEMLDCLSGLLDSDRGSVTLVER----GDKT-PLKRNPDFHLFAAMNPSTDVGKRELPVGLRNRFTEI-YVPELDPGVNDSQLNPIRVTDREDLAILIRAYLLPL 696
BLAST of Midasin vs. TrEMBL
Match: G7YPI3 (Midasin OS=Clonorchis sinensis OX=79923 GN=CLF_105889 PE=3 SV=1) HSP 1 Score: 336.265 bits (861), Expect = 2.026e-137 Identity = 183/393 (46.56%), Postives = 241/393 (61.32%), Query Frame = 2
Query: 86 SVVTIRDLLSWVNFINISIGNKTS--------------LYQSIFHGAYMVIIDSINDKLNKSFYE---------ELLIKKLDYL-VKSSFPDIVECL-----------DFQSSRFELSVKENKVGIDPFYIELGSHSDWXXXXXXXXXXKNFQYSFNSKTTGSNVYRLFRALQVSKKPLLLEGSPGVGKTSLVTQLAKLVGFKVVRINLSEQTDANDLFGCFLPNENGKAGELSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPEINQKFMVEPGKTRLFACQNPVSEGSGRKGLPKSFVNRFTQVYMSSLSDSDYRNILNCLYPAIARETIGFIVEFVQNLREHVVVMKKFGTKGSPWEFNLRDLCRWCDLMVKHQH 1159
S TIRDLL+W+ F+ S+ +++S L + HGA +V +D+++ K E + L ++L+ + ++ P VE L + F L+ G DPFYI+ G SD N + N +++F++ T N+ RL RALQV K+ LLLEGSPGVGKTSLV LAK G VVRINLSE T+A+DL GC LP E G WRDGPLLQ L+ G WI+LDEMNLASQSVLECLNACLDHRGE++IPE+ F ++PG TRLFACQNPV EG GRKGLPKSF+NRFTQV++ L+ +D I+ LYP + + +V F L + V V ++FGT+G PWEFNLRDL RW +L++K ++
Sbjct: 1461 SPPTIRDLLAWIQFME-SVCSRSSNPASTPPRLSSSIDLLSACLHGACLVFLDAVDCKPIGGILEGPSLAAQGVKFLRERLESIALEHDLP--VENLFIAAKYTCAGDNSMDGGFGLTSDGRMFGADPFYIDTGPSSD-------PNLALNSRFNFSAPTPAVNLRRLLRALQVPKRALLLEGSPGVGKTSLVLALAKATGHDVVRINLSEATEASDLLGCDLPVEGAAPGVFGWRDGPLLQGLREGRWIVLDEMNLASQSVLECLNACLDHRGELYIPELGATFKIQPGATRLFACQNPVREGGGRKGLPKSFLNRFTQVFLQPLTRADQEFIMRQLYPRLPAPVVQLMVHFNWKLADEVNVAREFGTQGGPWEFNLRDLMRWAELILKGEN 1843 HSP 2 Score: 187.193 bits (474), Expect = 2.026e-137 Identity = 106/268 (39.55%), Postives = 161/268 (60.07%), Query Frame = 1
Query: 1159 RTKEDQDACGKVYNELADK---SRFARFIPSGLDFYITDQVLKIGQTQLSVNIANNTDNSKQFNKHLLLAGQLAILESMMLTVDMGWLTVLVGPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGSFEQQKEGGKFKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGLVYGDRIEEIKPHKNFRLFLILDPDCGG-----EISKAMRNRGLEICIIPENRELTLTDNDLLAILITI-YKLSVKNA 1935
RT+ D+ +++N + + + + P G + + +L+IG T + D SK N LLL Q + LES++ ++MGW+ +LVGP G GK T+ + + + G+ L +M M+ DT +LLGS EQ + GG F W+DS LV+ +R G W+++++ CS SVLDRLN +LEP+G L+L+ERGL R+ +KPH NFRL LI+D G +S+AMRNRG+EI + ++L++T DL IL +I + SV NA
Sbjct: 1862 RTEADKRKIAELWNSVCRERGATDMVYYKPLG-RVSLVNNILRIGVT--GFDTVPTVDPSKLPNL-LLLDHQRSQLESLLKILEMGWMAILVGPPGCGKRTLTNICATLVGHPLSTMHMSPTADTVELLGSLEQHEAGGHFCWIDSPLVKGMRHGHWVVIESAQLCSPSVLDRLNSVLEPDGELILSERGLDSNGRLIRLKPHPNFRLILIVDEVSGAGAGSTGVSRAMRNRGVEIAVY---KDLSMTTVDLDRILTSIGIRNSVVNA 2122 HSP 3 Score: 233.417 bits (594), Expect = 5.960e-57 Identity = 128/279 (45.88%), Postives = 184/279 (65.95%), Query Frame = 1
Query: 9139 FTWSQYESKTEYLSRQLCEMLRIVLEPTKANKLGGAYRTGKRLNMKKVIPYIASGFRKDKIWLRRNKPSQREYQVIIGVDNSKSMAYNVNHQLFFESLSIVLNGLTFLEVGQVGLCKFGAKAEVVLPLYEKFSSEIGDRLIETFDFSDKCSKFIPILEQSIKLFEENQNFLSSSAVRXXXXXXXXXXXXXXXXXXXXPKFISLIREAHNKGIFVVVILLDHADSKDSILNLKMVIDDGCDFVRFLDVFPLQNYTVLQDVTKLPTLLTDVLRQWFELSSS 9975
F WS +++ L+RQLCE LR+VLEPTKA ++ G YRTGKR+NM+K+IPY+AS FRKDKIWLRR++PSQREY+++I VD+S SM+ N+ Q+ F++L+ V+ L LEVG++G+C FG ++ L + + + G ++ F F + I +L+ +IK + L ++ SQLLL+LSDG SED DPK + +R A + +F V ++LD K SI +L+ +G V ++D FPL Y VL+DVT LP LL+D LRQWFEL +S
Sbjct: 4812 FEWSACSARSAGLARQLCESLRLVLEPTKAARMRGDYRTGKRINMRKIIPYLASHFRKDKIWLRRSQPSQREYRILIAVDDSSSMSDNLCRQMTFDALATVVTALNLLEVGRIGVCSFGESVRILHDLQDTWIGDAGANVLAQFTFKQSRTSLIQLLQSAIKAMQMCN--LPTTTGSMPSQLLLILSDGVFSEDPQDPKVQAAVRLARDSRLFPVCLILDDVRKKHSIFDLRRYTSNG-KLVPYMDTFPLPFYLVLRDVTALPQLLSDALRQWFELEAS 5087 HSP 4 Score: 114.775 bits (286), Expect = 5.361e-21 Identity = 76/219 (34.70%), Postives = 111/219 (50.68%), Query Frame = 2
Query: 512 PLLLEGSPGVGKTSLVTQLAKLVGFKVVRINLSEQTDANDLFGCFLPN--------ENGKAGELSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPEINQKFMVEPGKTRLFACQNPVSEGSGRKGLPKSFVNRFTQVYMSSLSDSDYRNILN--CLYPAIARETIGFIVEFVQNLREHVVVMKKFGTKGSPWEFNLRDLCRWCD 1138
P+LL+G VGKTSL+T LA+ +G RIN E T+ G + ++G L ++ GPL+QA++ G WI+LDE+NLA +LE LN LD +FIP+ + P + RLFA QNP +GRK L ++ NRF +++ + + IL C A+ + +VE + L+ F K S LRDL RW +
Sbjct: 890 PILLQGETSVGKTSLITYLAERIGQVCYRINNHEHTEQQTYLGTYTVASATAQANMKDGTPPPLVFQHGPLVQAMRLGHWIILDELNLAPTEILEALNRVLDDNRTLFIPDTQEVIRAHP-QFRLFATQNPPGLYAGRKVLSRALRNRFIELHFDPIPRGELEVILEKRC---ALPQSRAHRLVEVMHRLQLARCQSNVFLGKDS--FITLRDLFRWAE 1102 HSP 5 Score: 79.337 bits (194), Expect = 2.594e-10 Identity = 54/156 (34.62%), Postives = 85/156 (54.49%), Query Frame = 1
Query: 1417 VLVGPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGSFEQQKEG---GKFKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGLVYGDRIEEIKPHKNFRLFLILDP--DCGGE--ISKAMRNRGLEICIIPENR 1863
+L G G GKT++V L+ TG+ + ++++ A + +DLLG + EG G F W D L+Q LR+G W++LD +N S SVL+ LN L+ G L + E G + +I+P RLF +P + GG + K+ NR ++ + P R
Sbjct: 1630 LLEGSPGVGKTSLVLALAKATGHDVVRINLSEATEASDLLGC-DLPVEGAAPGVFGWRDGPLLQGLREGRWIVLDEMNLASQSVLECLNACLDHRGELYIPELGATF-----KIQPGAT-RLFACQNPVREGGGRKGLPKSFLNRFTQVFLQPLTR 1778 HSP 6 Score: 77.7962 bits (190), Expect = 7.452e-10 Identity = 54/167 (32.34%), Postives = 80/167 (47.90%), Query Frame = 1
Query: 1417 VLVGPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGSFEQQK-----------EGGKFKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGLVYGD----RIEEIKPHKNFRLFLILDPDCGG-----EISKAMRNRGLEI-CIIP 1854
+LVG GKTT+ +L + + L ++ + + D LG + +G F+WVD LV A+ +G+ +LD ++ +VL+RLN LLEP L L ER G ++ H FRL ++P GG E+S A+RNR EI C P
Sbjct: 1225 LLVGETSCGKTTVCQLFAALKHQKLHCVNCHQYTEAGDFLGGLRPIRRVSSQDGAEVDDGRLFEWVDGPLVVAMSRGEMFLLDEISLADDAVLERLNSLLEPERRLNLAERSEGAGSIGVLETNDLTAHIGFRLVATMNP--GGDYGKKELSPALRNRFTEIWCPTP 1389 HSP 7 Score: 76.2554 bits (186), Expect = 2.159e-9 Identity = 62/280 (22.14%), Postives = 117/280 (41.79%), Query Frame = 1
Query: 5377 DIYQDPIDVDNLLQAQKILPNANVSVTKLLGIYPEHTTXXXXXXXXXXXXXXSGDYSLIVFINNVERLIHEIQQWEVVAARHVSLIQHVDELNGLLVSWRHQELTMWENTISTANWQLERAVSREFFNHYNLIVQESNKIQYDNDF---ESINLILEPILNSLLNSTVGNFEYRLKTMKLSIESLISNREIDYRFKDRLKNILLNHYDFYRLYLGNVQEHLSSEYKRIGKELRDFIKTKRWGKTP---PKITDVLKCHKIVFKYKRQWSGIINQPCTKFF 6198
D+Y+DPI +D + +L + V ++L +P + L+HE WE A+RHVSL+ + E+ +L+ WR EL W +T+ +A + +F+ +++ ++ D+ +S + E ++ + N +G F R + + +L + + ++ K I+ N FY +L VQ + K I KELR + +WG ++V +C + + K R W ++ +P F
Sbjct: 3309 DVYRDPIPLDAASKLLTVLEHLERRVRQILHEWPGQPS-----------------------------LVHE---WEKNASRHVSLMDQIKEVCEILIEWRKMELKCWLSTLDSATSAFSNRAATLWFHLHDVFLRPPGHATSDSTVGKTQSESDRCEVLVELMENGPLGEFPMRWRLLAALRTALDIWPGLPTQDRETNKRIVGNVVWFYGQFLSTVQNEIKCLRKPIEKELRGVVHITKWGDYACFWSVKSNVDRCKRTIHKQVRNWETVLRRPVRPLF 3556 HSP 8 Score: 72.7886 bits (177), Expect = 2.107e-8 Identity = 61/237 (25.74%), Postives = 107/237 (45.15%), Query Frame = 2
Query: 506 KKPLLLEGSPGVGKTSLVTQLAKLVGFKVVRINLSEQTDANDLFGCFLPNENGKAGELSWRDGPLLQALKAGDWILLDEMNLAS---QSVLECL---NACLDHRGEIFIPE--------------INQKFMVEPGKTRLFACQNPVSEGSGRKGLPKSFVNRFTQVYM-SSLSDSDYRNILNCLYPAIARETIGFIVEFVQNLREHVVVMKKF-GTKGSPWEFNLRDLCRWCDLMVK 1150
+KP+LL G G GKT + +A + V R+ +SEQTD+ L G + E G+ WR GPLL +L G W++L++++ Q +L L N HR ++ P + ++ ++ C NP +E F++R V M +L+D R +++ LYP++ + I+ +RE + F G++ + RDL ++C + +
Sbjct: 95 RKPVLLAGPVGCGKTRCIDFVACVRKASVCRVQISEQTDSKSLLGAYCCTET--PGQFVWRPGPLLTSLTQGSWLVLEDVDRGPADLQMLLAPLFKPNHSSCHRLDLIDPTTGLPVDQHRNFRILMTRRLVLSRTNEWRMECDNPCAE----------FIDRHCSVIMLPALNDDSLRQLVSRLYPSL-HTLLDRILTIYGCVREDLESSSAFTGSR----TISPRDLLKFCARIAR 314 HSP 9 Score: 70.8626 bits (172), Expect = 8.392e-8 Identity = 50/169 (29.59%), Postives = 86/169 (50.89%), Query Frame = 2
Query: 518 LLEGSPGVGKTSLVTQLAKLVGFKVVRINLSEQTDANDLFGCFLPNENGKAGELSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPE----INQKFM-VEPGKT-RLFACQNPVS-EGSGRKGLPKSFVNRFTQVYMS---SLSDSDYRNILNCL 994
+L G PG GK +L A LVG + +++S D +L G +E G G W D PL++ ++ G W++++ L S SVL+ LN+ L+ GE+ + E N + + ++P RL + VS G+G G+ ++ NR ++ + S++ D IL +
Sbjct: 1947 ILVGPPGCGKRTLTNICATLVGHPLSTMHMSPTADTVELLGSLEQHEAG--GHFCWIDSPLVKGMRHGHWVVIESAQLCSPSVLDRLNSVLEPDGELILSERGLDSNGRLIRLKPHPNFRLILIVDEVSGAGAGSTGVSRAMRNRGVEIAVYKDLSMTTVDLDRILTSI 2113 HSP 10 Score: 68.9366 bits (167), Expect = 3.100e-7 Identity = 66/230 (28.70%), Postives = 108/230 (46.96%), Query Frame = 2
Query: 506 KKPLLLEGSPGVGKTSLVTQLAKLVGFKVVRINLSEQTDANDLFGCFLP-----NENGKA---GEL-SWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHR------------GEIFIPEINQKFMVEPGKTRLFACQNPVSEGSGRKGLPKSFVNRFTQVYMSSLSDSDYRNILNCLYPAIARETIGFIVEFVQNLREHVVVMKKFGTKGSPWEFNLRDLCRW 1132
K+P+LL G GKT++ A L K+ +N + T+A D G P +++G G L W DGPL+ A+ G+ LLDE++LA +VLE LN+ L+ G I + E N + RL A NP + G+K L + NRFT+++ + + + NI + +P + + + IV V NL+ H+ +++ + + D W
Sbjct: 1221 KEPILLVGETSCGKTTVCQLFAALKHQKLHCVNCHQYTEAGDFLGGLRPIRRVSSQDGAEVDDGRLFEWVDGPLVVAMSRGEMFLLDEISLADDAVLERLNSLLEPERRLNLAERSEGAGSIGVLETND--LTAHIGFRLVATMNPGGD-YGKKELSPALRNRFTEIWCPTPAFTSP-NIED--FPTSSSD-LQLIV--VHNLKLHLPLLRTSAHLAERLSYAMVDFASW 1441 HSP 11 Score: 68.5514 bits (166), Expect = 4.996e-7 Identity = 55/177 (31.07%), Postives = 82/177 (46.33%), Query Frame = 2
Query: 659 NGKAGELSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLD-HRGEIFIPEINQKFMVEPGKT-RLFACQNPVSEGSGRKGLPKSFVNRFTQVYMSSLSDSDYRNILNCLYPAIARETIGF------------------IVEFVQNLREHVVVMKKFGTKGSPWEFNLRDLCR 1129
N A ++ +G L++A++ GDW+LLDE+NLA +L+CL+ LD G + + E K ++ LFA NP S G++ LP NRFT++Y+ L + LN + A RE + +V LR+ V G P F+LR LCR
Sbjct: 578 NQSAVGFAFIEGALVRAVQDGDWVLLDEINLAPAEMLDCLSGLLDSDHGSVTLVERGDKTPLKRNPDFHLFAAMNP-STDVGKRELPVGLRNRFTEIYVPELDPGINDSQLNPIR-ATDREDLAILIRAYLLPLNPSPTQLTTVVRLYAALRQAAVEGLVDGVGQKP-HFSLRTLCR 751 HSP 12 Score: 64.6994 bits (156), Expect = 5.911e-6 Identity = 49/139 (35.25%), Postives = 77/139 (55.40%), Query Frame = 1
Query: 1570 FKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPN-GSLLLTERGLVYGDRIEEIKPHKNFRLFLILDP--DCGG-EISKAMRNRGLEICIIPE---------NRELTLTDNDLLAILITIYKLSVKNADSLL 1947
F +++ LV+A++ GDW++LD +N A +LD L+GLL+ + GS+ L ER GD+ +K + +F LF ++P D G E+ +RNR EI +PE + TD + LAILI Y L + + + L
Sbjct: 584 FAFIEGALVRAVQDGDWVLLDEINLAPAEMLDCLSGLLDSDHGSVTLVER----GDKT-PLKRNPDFHLFAAMNPSTDVGKRELPVGLRNRFTEI-YVPELDPGINDSQLNPIRATDREDLAILIRAYLLPLNPSPTQL 716
BLAST of Midasin vs. TrEMBL
Match: A0A419Q950 (Midasin OS=Clonorchis sinensis OX=79923 GN=MDN1 PE=3 SV=1) HSP 1 Score: 335.88 bits (860), Expect = 2.353e-137 Identity = 184/391 (47.06%), Postives = 242/391 (61.89%), Query Frame = 2
Query: 86 SVVTIRDLLSWVNFINISIGNKTS--------------LYQSIFHGAYMVIIDSINDKLNKSFYE---------ELLIKKLDYL-VKSSFPDIVECLDFQSSR------------FELSVKENKVGIDPFYIELGSHSDWXXXXXXXXXXKNFQYSFNSKTTGSNVYRLFRALQVSKKPLLLEGSPGVGKTSLVTQLAKLVGFKVVRINLSEQTDANDLFGCFLPNENGKAGELSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPEINQKFMVEPGKTRLFACQNPVSEGSGRKGLPKSFVNRFTQVYMSSLSDSDYRNILNCLYPAIARETIGFIVEFVQNLREHVVVMKKFGTKGSPWEFNLRDLCRWCDLMVK 1150
S TIRDLL+W+ F+ S+ +++S L + HGA +V +D+++ K E + L ++L+ + ++ P VE L F +++ F L+ G DPFYI+ G SD N + N +++F++ T N+ RL RALQV K+ LLLEGSPGVGKTSLV LAK G VVRINLSE T+A+DL GC LP E G WRDGPLLQ L+ G WI+LDEMNLASQSVLECLNACLDHRGE++IPE+ F ++PG TRLFACQNPV EG GRKGLPKSF+NRFTQV++ L+ +D I+ LYP + + +V F L + V V ++FGT+G PWEFNLRDL RW +L++K
Sbjct: 1461 SPPTIRDLLAWIQFME-SVCSRSSNPASTPPRLSSSIDLLSACLHGACLVFLDAVDCKPIGGILEGPSLAAQGVKFLRERLESIALEHDLP--VENL-FIAAKYTCAGDNSVDGGFGLTSDGRMFGADPFYIDTGPSSD-------PNLALNSRFNFSAPTPAVNLRRLLRALQVPKRALLLEGSPGVGKTSLVLALAKATGHDVVRINLSEATEASDLLGCDLPVEGAAPGVFGWRDGPLLQGLREGRWIVLDEMNLASQSVLECLNACLDHRGELYIPELGATFKIQPGATRLFACQNPVREGGGRKGLPKSFLNRFTQVFLQPLTRADQEFIMRQLYPRLPAPVVQLMVHFNWKLADEVNVAREFGTQGGPWEFNLRDLMRWAELILK 1840 HSP 2 Score: 187.578 bits (475), Expect = 2.353e-137 Identity = 106/268 (39.55%), Postives = 161/268 (60.07%), Query Frame = 1
Query: 1159 RTKEDQDACGKVYNELADK---SRFARFIPSGLDFYITDQVLKIGQTQLSVNIANNTDNSKQFNKHLLLAGQLAILESMMLTVDMGWLTVLVGPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGSFEQQKEGGKFKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGLVYGDRIEEIKPHKNFRLFLILDPDCGG-----EISKAMRNRGLEICIIPENRELTLTDNDLLAILITI-YKLSVKNA 1935
RT+ D+ +++N + + + + P G + + +L+IG T + D SK N LLL Q + LES++ ++MGW+ +LVGP G GK T+ + + + G+ L +M M+ DT +LLGS EQ + GG F W+DS LV+ +R G W+++++ CS SVLDRLN +LEP+G L+L+ERGL R+ +KPH NFRL LI+D G +S+AMRNRG+EI + ++L++T DL IL +I + SV NA
Sbjct: 1862 RTEADKRKIAELWNSVCRERGATDMVYYKPLG-RVSLVNNILRIGVT--GFDTVPTVDASKLPNL-LLLDHQRSQLESLLKILEMGWMAILVGPPGCGKRTLTNICATLVGHPLSTMHMSPTADTVELLGSLEQHEAGGHFCWIDSPLVKGMRHGHWVVIESAQLCSPSVLDRLNSVLEPDGELILSERGLDSNGRLIRLKPHPNFRLILIVDEVSGAGAGSTGVSRAMRNRGVEIAVY---KDLSMTTVDLDRILTSIGIRNSVVNA 2122 HSP 3 Score: 114.39 bits (285), Expect = 5.804e-21 Identity = 76/219 (34.70%), Postives = 111/219 (50.68%), Query Frame = 2
Query: 512 PLLLEGSPGVGKTSLVTQLAKLVGFKVVRINLSEQTDANDLFGCFLPN--------ENGKAGELSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPEINQKFMVEPGKTRLFACQNPVSEGSGRKGLPKSFVNRFTQVYMSSLSDSDYRNIL--NCLYPAIARETIGFIVEFVQNLREHVVVMKKFGTKGSPWEFNLRDLCRWCD 1138
P+LL+G VGKTSL+T LA+ +G RIN E T+ G + ++G L ++ GPL+QA++ G WI+LDE+NLA +LE LN LD +FIP+ + P + RLFA QNP +GRK L ++ NRF +++ + + IL C A+ + +VE + L+ F K S LRDL RW +
Sbjct: 890 PILLQGETSVGKTSLITYLAERIGQVCYRINNHEHTEQQTYLGTYTVASATAQANMKDGTPPPLVFQHGPLVQAMRLGHWIILDELNLAPTEILEALNRVLDDNRTLFIPDTQEVIRAHP-QFRLFATQNPPGLYAGRKVLSRALRNRFIELHFDPIPRGELEVILEKRC---ALPQSRAHRLVEVMHRLQLARCQSNVFLGKDS--FITLRDLFRWAE 1102 HSP 4 Score: 89.7373 bits (221), Expect = 1.570e-13 Identity = 66/280 (23.57%), Postives = 129/280 (46.07%), Query Frame = 1
Query: 5377 DIYQDPIDVDNLLQAQKILPNANVSVTKLLGIYPEHTTXXXXXXXXXXXXXXSGDYSLIVFINNVERLIHEIQQWEVVAARHVSLIQHVDELNGLLVSWRHQELTMWENTISTANWQLERAVSREFFNHYNLIVQESNKIQYDNDF---ESINLILEPILNSLLNSTVGNFEYRLKTMKLSIESLISNREIDYRFKDRLKNILLNHYDFYRLYLGNVQEHLSSEYKRIGKELRDFIKTKRWGKTP---PKITDVLKCHKIVFKYKRQWSGIINQPCTKFF 6198
D+Y+DPI +D + +L + V ++L +P +L+++ I + L+ F+ VE + E+ +WE A+RHVSL+ + E+ +L+ WR EL W +T+ +A + +F+ +++ ++ D+ +S + E ++ + N +G F R + + +L + + ++ K I+ N FY +L VQ + K I KELR + +WG ++V +C + + K R W ++ +P F
Sbjct: 3570 DVYRDPIPLDAASKLLTVLEHLERRVRQILHEWPGQPSLVHLITLIRRLEGFHVSDPLMKFLTGVEMIWQEMHEWEKNASRHVSLMDQIKEVCEILIEWRKMELKCWLSTLDSATSAFSNRAATLWFHLHDVFLRPPGHATSDSTVGKTQSESDRCEVLVELMENGPLGEFPMRWRLLAALRTALDIWPGLPTQDRETNKRIVGNVVWFYGQFLSTVQNEIKCLRKPIEKELRGVVHITKWGDYACFWSVKSNVDRCKRTIHKQVRNWETVLRRPVRPLF 3849 HSP 5 Score: 78.9518 bits (193), Expect = 2.828e-10 Identity = 54/156 (34.62%), Postives = 85/156 (54.49%), Query Frame = 1
Query: 1417 VLVGPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGSFEQQKEG---GKFKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGLVYGDRIEEIKPHKNFRLFLILDP--DCGGE--ISKAMRNRGLEICIIPENR 1863
+L G G GKT++V L+ TG+ + ++++ A + +DLLG + EG G F W D L+Q LR+G W++LD +N S SVL+ LN L+ G L + E G + +I+P RLF +P + GG + K+ NR ++ + P R
Sbjct: 1630 LLEGSPGVGKTSLVLALAKATGHDVVRINLSEATEASDLLGC-DLPVEGAAPGVFGWRDGPLLQGLREGRWIVLDEMNLASQSVLECLNACLDHRGELYIPELGATF-----KIQPGAT-RLFACQNPVREGGGRKGLPKSFLNRFTQVFLQPLTR 1778 HSP 6 Score: 77.7962 bits (190), Expect = 7.923e-10 Identity = 54/167 (32.34%), Postives = 80/167 (47.90%), Query Frame = 1
Query: 1417 VLVGPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGSFEQQK-----------EGGKFKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGLVYGD----RIEEIKPHKNFRLFLILDPDCGG-----EISKAMRNRGLEI-CIIP 1854
+LVG GKTT+ +L + + L ++ + + D LG + +G F+WVD LV A+ +G+ +LD ++ +VL+RLN LLEP L L ER G ++ H FRL ++P GG E+S A+RNR EI C P
Sbjct: 1225 LLVGETSCGKTTVCQLFAALKHQKLHCVNCHQYTEAGDFLGGLRPIRRVSSQDGAEVDDGRLFEWVDGPLVVAMSRGEMFLLDEISLADDAVLERLNSLLEPERRLNLAERSEGAGSIGVLETNDLTAHIGFRLVATMNP--GGDYGKKELSPALRNRFTEIWCPTP 1389 HSP 7 Score: 73.1738 bits (178), Expect = 1.992e-8 Identity = 61/237 (25.74%), Postives = 107/237 (45.15%), Query Frame = 2
Query: 506 KKPLLLEGSPGVGKTSLVTQLAKLVGFKVVRINLSEQTDANDLFGCFLPNENGKAGELSWRDGPLLQALKAGDWILLDEMNLAS---QSVLECL---NACLDHRGEIFIPE--------------INQKFMVEPGKTRLFACQNPVSEGSGRKGLPKSFVNRFTQVYM-SSLSDSDYRNILNCLYPAIARETIGFIVEFVQNLREHVVVMKKF-GTKGSPWEFNLRDLCRWCDLMVK 1150
+KP+LL G G GKT + +A + V R+ +SEQTD+ L G + E G+ WR GPLL +L G W++L++++ Q +L L N HR ++ P + ++ ++ C NP +E F++R V M +L+D R +++ LYP++ + I+ +RE + F G++ + RDL ++C + +
Sbjct: 95 RKPVLLAGPVGCGKTRCIDFVACVQKASVCRVQISEQTDSKSLLGAYCCTET--PGQFVWRPGPLLTSLTQGSWLVLEDVDRGPADLQMLLAPLFKPNHSSCHRLDLIDPTTGLLVDQHRNFRILMTRRLVLSRTNEWRMECDNPCAE----------FIDRHCSVIMLPALNDDSLRQLVSRLYPSL-HTLLDRILTIYGCVREDLESSSAFTGSR----TISPRDLLKFCARIAR 314 HSP 8 Score: 70.8626 bits (172), Expect = 8.844e-8 Identity = 50/169 (29.59%), Postives = 86/169 (50.89%), Query Frame = 2
Query: 518 LLEGSPGVGKTSLVTQLAKLVGFKVVRINLSEQTDANDLFGCFLPNENGKAGELSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPE----INQKFM-VEPGKT-RLFACQNPVS-EGSGRKGLPKSFVNRFTQVYMS---SLSDSDYRNILNCL 994
+L G PG GK +L A LVG + +++S D +L G +E G G W D PL++ ++ G W++++ L S SVL+ LN+ L+ GE+ + E N + + ++P RL + VS G+G G+ ++ NR ++ + S++ D IL +
Sbjct: 1947 ILVGPPGCGKRTLTNICATLVGHPLSTMHMSPTADTVELLGSLEQHEAG--GHFCWIDSPLVKGMRHGHWVVIESAQLCSPSVLDRLNSVLEPDGELILSERGLDSNGRLIRLKPHPNFRLILIVDEVSGAGAGSTGVSRAMRNRGVEIAVYKDLSMTTVDLDRILTSI 2113 HSP 9 Score: 68.1662 bits (165), Expect = 5.178e-7 Identity = 66/230 (28.70%), Postives = 108/230 (46.96%), Query Frame = 2
Query: 506 KKPLLLEGSPGVGKTSLVTQLAKLVGFKVVRINLSEQTDANDLFGCFLP-----NENGKA---GEL-SWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHR------------GEIFIPEINQKFMVEPGKTRLFACQNPVSEGSGRKGLPKSFVNRFTQVYMSSLSDSDYRNILNCLYPAIARETIGFIVEFVQNLREHVVVMKKFGTKGSPWEFNLRDLCRW 1132
K+P+LL G GKT++ A L K+ +N + T+A D G P +++G G L W DGPL+ A+ G+ LLDE++LA +VLE LN+ L+ G I + E N + RL A NP + G+K L + NRFT+++ + + + NI + +P + + + IV V NL+ H+ +++ + + D W
Sbjct: 1221 KEPILLVGETSCGKTTVCQLFAALKHQKLHCVNCHQYTEAGDFLGGLRPIRRVSSQDGAEVDDGRLFEWVDGPLVVAMSRGEMFLLDEISLADDAVLERLNSLLEPERRLNLAERSEGAGSIGVLETND--LTAHIGFRLVATMNPGGD-YGKKELSPALRNRFTEIWCPTPAFTSP-NIKD--FPTSSSD-LQLIV--VHNLKLHLPLLRTSPHLAERLSYAMVDFASW 1441 HSP 10 Score: 68.1662 bits (165), Expect = 5.920e-7 Identity = 53/167 (31.74%), Postives = 78/167 (46.71%), Query Frame = 2
Query: 689 DGPLLQALKAGDWILLDEMNLASQSVLECLNACLD-HRGEIFIPEINQKFMVEPGKT-RLFACQNPVSEGSGRKGLPKSFVNRFTQVYMSSLSDSDYRNILNCLYPAIARETIGF------------------IVEFVQNLREHVVVMKKFGTKGSPWEFNLRDLCR 1129
+G L++A++ GDW+LLDE+NLA +L+CL+ LD G + + E K ++ LFA NP S G++ LP NRFT++Y+ L + LN + A RE + +V LR+ V G P F+LR LCR
Sbjct: 588 EGALVRAVQDGDWVLLDEINLAPAEMLDCLSGLLDSDHGSVTLVERGDKAPLKRNPDFHLFAAMNP-STDVGKRELPVGLRNRFTEIYVPELDPGINDSQLNPIR-ATDREDLAILIRAYLLPLNPSPTQLTTVVRLYAALRQAAVEGLVDGVGQKP-HFSLRTLCR 751 HSP 11 Score: 64.6994 bits (156), Expect = 6.604e-6 Identity = 49/139 (35.25%), Postives = 77/139 (55.40%), Query Frame = 1
Query: 1570 FKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPN-GSLLLTERGLVYGDRIEEIKPHKNFRLFLILDP--DCGG-EISKAMRNRGLEICIIPE---------NRELTLTDNDLLAILITIYKLSVKNADSLL 1947
F +++ LV+A++ GDW++LD +N A +LD L+GLL+ + GS+ L ER GD+ +K + +F LF ++P D G E+ +RNR EI +PE + TD + LAILI Y L + + + L
Sbjct: 584 FAFIEGALVRAVQDGDWVLLDEINLAPAEMLDCLSGLLDSDHGSVTLVER----GDKA-PLKRNPDFHLFAAMNPSTDVGKRELPVGLRNRFTEI-YVPELDPGINDSQLNPIRATDREDLAILIRAYLLPLNPSPTQL 716
BLAST of Midasin vs. TrEMBL
Match: A0A0R3T203 (Midasin OS=Rodentolepis nana OX=102285 PE=3 SV=2) HSP 1 Score: 316.62 bits (810), Expect = 2.277e-132 Identity = 181/405 (44.69%), Postives = 236/405 (58.27%), Query Frame = 2
Query: 95 TIRDLLSWVNFI-NISIG----NKTSLYQSIFHGAYMVIIDSINDKLNKS----------FYEE--------------------LLIKKL--DYLVKSSFPDIVECLDFQSSRF-------------ELSVKENKVGIDPFYIELGSHSDWXXXXXXXXXXKNFQYSFNSKTTGSNVYRLFRALQVSKKPLLLEGSPGVGKTSLVTQLAKLVGFKVVRINLSEQTDANDLFGCFLPNENGKAGELSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPEINQKFMVEPGKTRLFACQNPVSEGSGRKGLPKSFVNRFTQVYMSSLSDSDYRNILNCLYPAIARETIGFIVEFVQNLREHVVVMKKF-GTKGSPWEFNLRDLCRWCDLMVKHQ 1156
TIRDLLSWV F+ N++ ++ S++ + HGA ++ +DS+ ++ S F +E L++ L + VK S PD+++ + + F EL G +PF+IE G L S +S ++ T SN+ RL RALQ+ K+ LLLEGSPGVGKTSLV+ LA+ G +VVRINLSE T+A+DLFGC LP E G +W DGPLLQAL+ G W+LLDEMNLASQSVLECLNACLDHRG I+IPE+ F V+P TRLFACQNPV EG GR+ LPKSF+NRFTQVY+ L+ SD IL +Y + I +V+F L++ V F G+ WEFNLRDLCRW DL++ Q
Sbjct: 1035 TIRDLLSWVEFMQNLATSRHDFSRASIFNACLHGAALIFLDSLEEEERSSDEHMEHEPYLFVDESSLQTSPETLYKFSPVGGINFLLRCLLTHFEVKESNPDLLDDIRTAAQEFVMGLNLQPGQCVPELKDSNKLYGCEPFFIETGPE---LSPKELVGSRAPINFSLSAVTPASNLCRLLRALQLPKRALLLEGSPGVGKTSLVSALARAAGHRVVRINLSEATEASDLFGCDLPVEGAGCGTFAWCDGPLLQALRHGHWVLLDEMNLASQSVLECLNACLDHRGSIYIPELGTTFHVKPRATRLFACQNPVEEGGGRRALPKSFLNRFTQVYLKPLTASDQIAILISIYNDLPVPHIEAMVKFNSMLQDAVERGDSFNAVAGTSWEFNLRDLCRWGDLLIAGQ 1436 HSP 2 Score: 190.274 bits (482), Expect = 2.277e-132 Identity = 144/489 (29.45%), Postives = 243/489 (49.69%), Query Frame = 1
Query: 1150 ASARTKEDQDACGKVYNELADKSRFAR-FIPSGLDFYITDQVLKIGQTQLSVNIANNTDNSKQFNKHLLLAGQLAILESMMLTVDMGWLTVLVGPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGSFEQQKEGGKFKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGLVYGDRIEEIKPHKNFRLFLILDPDC------GGEISKAMRNRGLEICIIPENRELTLTDNDLLAILITIYKLSVKNADSLLRFHKTMI-LAQSETPQIKIGN-------INSLIQAAKLIK---DGYSLDQSLRSVYGDIQMLFNVRDDVNRFINDCNEE------VIDYQEIEQDDFSTCSSLF----NIITEPKKSLF----------LRHVKALKKTLKTQQVVNKNEKLIA-CQLFLQYSTLDDMPNRLEF-VE--FDVDLATVCKKIADAIPKFLNSTNRYLPIDFRWLPNW 2490
A R+ ED+ +++N +A+++ R +IP G + +IG + SVN A T + L+L LE ++ + GW+++L+GP G GK ++V L + I H+ +++++ + DT +LLG+FEQ + GG F W+DS LV+ +R+G W++L+ CS SVLDRLN LLEP G LL++ERGL + +KPH FRL L++D + IS+AMRNRG+E+ + +LT DL +++ LS ++LL F ++I + P++ + + +L+ AA++I+ G LD +L G + ++ + R+ + + + +ID Q D + L N + +L L HV L ++ +VN+ ++ C L S +P + F VE F + +TV + +I +F +S N ID RW+P W
Sbjct: 1453 ARMRSNEDKTRVIELWNGVAEETGLGRCYIPRGCTYPPIGNKFQIGLSTWSVNGAE-TRTEIIEDSLLILNQHRPYLEMLLKILQHGWMSILIGPRGAGKRSLVHLAAQIVHRHVATVALSPSADTVELLGAFEQHENGGIFAWIDSPLVRGIREGHWVVLENAQLCSPSVLDRLNSLLEPGGDLLISERGLDANGELVRLKPHSEFRLILVVDDNTPTVASYSNNISRAMRNRGVEMVL---TNDLTFHKEDLQRLILNT-GLSPACVNALLTFESSVIQRMHTLAPEVTASSLPPRKPPVGALLSAARVIQQITSGKELD-ALMGSEGAEETTEDIAPWMVRWKGEDSRKKAFAKALIDVYSRRQTDHNVSQVLIKAICNFVDNELSTLLVSDQIPLQPRLNHVSELLSSISP--LVNQWRHVLEHCSAVLSSSDNSLLPILIRFSVERGFKIPSSTV----SLSIKQFASSINEVDNIDLRWIPGW 1929 HSP 3 Score: 191.815 bits (486), Expect = 2.411e-44 Identity = 105/229 (45.85%), Postives = 152/229 (66.38%), Query Frame = 1
Query: 9145 WSQYESKTEYLSRQLCEMLRIVLEPTKANKLGGAYRTGKRLNMKKVIPYIASGFRKDKIWLRRNKPSQREYQVIIGVDNSKSMAYNVNHQLFFESLSIVLNGLTFLEVGQVGLCKFGAKAEVVLPLYEKFSSEIGDRLIETFDFSDKCSKFIPILEQSIKLFEENQNFLSSSAVRXXXXXXXXXXXXXXXXXXXXPKFISLIREAHNKGIFVVVILLDHADSKDSILNL 9831
WS Y +++ LS QLCE LR VLEPT+A+++ G +RTGKRLNM+K++PY+AS FRKDKIW+RR +P+QR+Y+++I VDNS SMA N+ + FE+L+ V+N L LE G++G+C FG EVV + E +++E+G ++ FDF + I +L+ SI L + ++S SQLLL+LSDG SED P + +R A + +FVV I++D K I NL
Sbjct: 4597 WSAYVTRSSGLSVQLCEALRQVLEPTRASRMKGDFRTGKRLNMRKIVPYLASQFRKDKIWMRRTQPNQRDYKILIAVDNSSSMADNLCKSMTFEALATVINALNLLEAGKIGVCSFGESVEVVRGMGEPWTNEMGASMLAKFDFKQSRTSLIQLLQTSISLMQVAGEGSATSRGVPASQLLLILSDGVFSEDPQSPALQAAVRLARDHHLFVVCIIIDDNKKKIEIGNL 4825 HSP 4 Score: 117.087 bits (292), Expect = 1.006e-21 Identity = 82/238 (34.45%), Postives = 116/238 (48.74%), Query Frame = 2
Query: 452 SKTTGSNVYRLFRALQVSKK-PLLLEGSPGVGKTSLVTQLAKLVGFKVVRINLSEQTDANDLFG----------CFLPNENGKAGELSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPEINQKFMVEPGKTRLFACQNPVSEGSGRKGLPKSFVNRFTQVYMSSLSDSDYRNILNCLYPAIARETIGFIVEFVQNLREHVVVMKKFGTKGSPWEFNLRDLCRW 1132
++T N+ L R + + P+LL+G VGKTSL+T LA VG RIN E TD G C A L +++G +QA+K G WI+LDE+NLA +LE LN LD E+FI E + P R+FA QNP +GRK L ++ NRF +++ +L ++ IL A+ R +VE + L H++ +G LRDL RW
Sbjct: 460 TETVRGNLKDLARVVSAAGSLPVLLQGETSVGKTSLITFLAARVGQVCHRINNHEHTDLQTYLGAYTAASATSACKNAGSGSGAVPLVFQEGIFVQAMKQGHWIILDELNLAPTEILEALNRVLDENRELFITETQEMVKAHP-HFRVFATQNPPGLYAGRKVLSRALRNRFVELHFDALPRAELEVILEH-RSALPRSRAAKMVEVMHQL--HLIRRSSDIFRGKDGFITLRDLFRW 693 HSP 5 Score: 97.4413 bits (241), Expect = 9.094e-16 Identity = 95/336 (28.27%), Postives = 155/336 (46.13%), Query Frame = 1
Query: 5377 DIYQDPIDVDNLLQAQKILPNANVSVTKLLGIYPEHTTXXXXXXXXXXXXXXSGDYSLIVFINNVERLIHEIQQWEVVAARHVSLIQHVDELNGLLVSWRHQELTMWENTISTANWQLERAVSREFFNHYNLIVQESNKIQYDNDFESINLILEPILNSLLNSTVGNFEYRLKTM-----KLSIESLISNREIDYRFKDRLKNILLNHYDFYRLYLGNVQEHLSSEYKRIGKELRDFIKTKRWGKTPPKIT---DVLKCHKIVFKYKRQWSGIINQ---PCTKFFVLTPINALKQVNLIDIGNCIES--DIDFSKSKLSD----WCATMYEYSREM 6333
D+Y DPI +A ++ V +LL +P+H L+ I IN + S + L +I E L+ E+Q+WE AARHVS+ + LL+ WR EL W ++ T+ + S +F+ ++ +Q ++ D + ++LE + N VG F R + + ++I +S+ +RF R ++ N FY ++ +V++ L E K I KE+ +FI +WG T +V KC K + K+ R W GI+ Q PC + T I + I I+S + DF +LS W + + E REM
Sbjct: 3026 DVYHDPIPKSEGKKAYAMMVAVRARVLQLLQEWPDHPALLKIITVINRMCAFSINDCLTKYITAFEMLLAEMQEWEKNAARHVSISDAFRSVCDLLIEWRKLELKCWMASLDTSTESAANSCSSLWFHLQSVFLQPTSTANIAED-DQCQILLE----FMENGPVGEFAARWRLLAALYNAIAIWPGLSD---THRFSAR--RMVGNVAWFYGQFIPHVKKFLLDEQKPIRKEMSNFISAMKWGDYIGFWTMKENVDKCKKTIHKHIRTWEGILRQSVKPCFDAAIKTSIEMPVPDTTMTILEHIQSIEEKDFGVFRLSPEMKTWLSKLTE-EREM 3350 HSP 6 Score: 96.2857 bits (238), Expect = 1.793e-15 Identity = 64/191 (33.51%), Postives = 105/191 (54.97%), Query Frame = 1
Query: 1417 VLVGPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGSFEQQKEG---GKFKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGLVYGDRIEEIKPHKNFRLFLILDP--DCGGE--ISKAMRNRGLEICIIPENRELTLTDNDLLAILITIYK-LSVKNADSLLRFHKTM 1965
+L G G GKT++V L+ G+ + ++++ A + +DL G + EG G F W D L+QALR G W++LD +N S SVL+ LN L+ GS+ + E G + +KP RLF +P + GG + K+ NR ++ + P LT +D +AILI+IY L V + +++++F+ +
Sbjct: 1223 LLEGSPGVGKTSLVSALARAAGHRVVRINLSEATEASDLFGC-DLPVEGAGCGTFAWCDGPLLQALRHGHWVLLDEMNLASQSVLECLNACLDHRGSIYIPELGTTF-----HVKPRAT-RLFACQNPVEEGGGRRALPKSFLNRFTQVYLKP------LTASDQIAILISIYNDLPVPHIEAMVKFNSML 1400 HSP 7 Score: 82.0333 bits (201), Expect = 3.763e-11 Identity = 60/177 (33.90%), Postives = 88/177 (49.72%), Query Frame = 1
Query: 1417 VLVGPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGSFEQQKEGGK----------FKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGLVYGDRI--EEIKPHKNFRLFLILDPDCGG-----EISKAMRNRGLEI-CIIP----ENRELTLTD 1881
+LVG G GKTTI ++ + + +L ++ + + D LG + F+WVD LV A+ QGD +LD ++ +VL+R+N LLEP + L ER GD + EEI +FRL ++P GG E+S A+RNR EI C P E + +TD
Sbjct: 808 LLVGETGCGKTTICQIFAALRKRNLLCVNCHQYTEAADFLGGLRPIRTHQPDDPNTTDDRLFEWVDGPLVLAMVQGDIFLLDEISLADDAVLERINSLLEPERKICLAER---CGDSLESEEITAVADFRLLATMNP--GGDYAKKELSPALRNRFTEIWCPSPTFIVEKSAIEMTD 979 HSP 8 Score: 72.7886 bits (177), Expect = 2.504e-8 Identity = 66/247 (26.72%), Postives = 110/247 (44.53%), Query Frame = 2
Query: 509 KPLLLEGSPGVGKTSLVTQLAKLVGFKVVRINLSEQTDANDLFGCFLPNENGKAGE--------LSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPE-----INQKFMVEPGKTRLFACQNPVSEGSGRKGLPKSFVNRFTQVY---------MSSLSDSDYRNILNC----------LYPAIARETIGFIVEFVQNLREHV---VVMKKFGTKGSPWEFNLRDLCRWCDLM 1144
+P+LL G G GKT++ A L ++ +N + T+A D G P + + W DGPL+ A+ GD LLDE++LA +VLE +N+ L+ +I + E + + + RL A NP + +K L + NRFT+++ S++ +D++ I+ L P +A+ + F F Q E V + K+ + P +RDL W + M
Sbjct: 805 EPILLVGETGCGKTTICQIFAALRKRNLLCVNCHQYTEAADFLGGLRPIRTHQPDDPNTTDDRLFEWVDGPLVLAMVQGDIFLLDEISLADDAVLERINSLLEPERKICLAERCGDSLESEEITAVADFRLLATMNPGGD-YAKKELSPALRNRFTEIWCPSPTFIVEKSAIEMTDWQAIVEHNLRRSDLLVDLTP-LAKAIVDFCWWFAQGRTEVVCGGMARKRRCRRPFP---TIRDLLSWVEFM 1046 HSP 9 Score: 70.4774 bits (171), Expect = 1.049e-7 Identity = 39/101 (38.61%), Postives = 58/101 (57.43%), Query Frame = 2
Query: 518 LLEGSPGVGKTSLVTQLAKLVGFKVVRINLSEQTDANDLFGCFLPNENGKAGELSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPE 820
+L G G GK SLV A++V V + LS D +L G F +ENG G +W D PL++ ++ G W++L+ L S SVL+ LN+ L+ G++ I E
Sbjct: 1542 ILIGPRGAGKRSLVHLAAQIVHRHVATVALSPSADTVELLGAFEQHENG--GIFAWIDSPLVRGIREGHWVVLENAQLCSPSVLDRLNSLLEPGGDLLISE 1640
BLAST of Midasin vs. TrEMBL
Match: A0A3P7SHE7 (VWFA domain-containing protein OS=Rodentolepis nana OX=102285 GN=HNAJ_LOCUS937 PE=4 SV=1) HSP 1 Score: 316.62 bits (810), Expect = 2.315e-132 Identity = 181/405 (44.69%), Postives = 236/405 (58.27%), Query Frame = 2
Query: 95 TIRDLLSWVNFI-NISIG----NKTSLYQSIFHGAYMVIIDSINDKLNKS----------FYEE--------------------LLIKKL--DYLVKSSFPDIVECLDFQSSRF-------------ELSVKENKVGIDPFYIELGSHSDWXXXXXXXXXXKNFQYSFNSKTTGSNVYRLFRALQVSKKPLLLEGSPGVGKTSLVTQLAKLVGFKVVRINLSEQTDANDLFGCFLPNENGKAGELSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPEINQKFMVEPGKTRLFACQNPVSEGSGRKGLPKSFVNRFTQVYMSSLSDSDYRNILNCLYPAIARETIGFIVEFVQNLREHVVVMKKF-GTKGSPWEFNLRDLCRWCDLMVKHQ 1156
TIRDLLSWV F+ N++ ++ S++ + HGA ++ +DS+ ++ S F +E L++ L + VK S PD+++ + + F EL G +PF+IE G L S +S ++ T SN+ RL RALQ+ K+ LLLEGSPGVGKTSLV+ LA+ G +VVRINLSE T+A+DLFGC LP E G +W DGPLLQAL+ G W+LLDEMNLASQSVLECLNACLDHRG I+IPE+ F V+P TRLFACQNPV EG GR+ LPKSF+NRFTQVY+ L+ SD IL +Y + I +V+F L++ V F G+ WEFNLRDLCRW DL++ Q
Sbjct: 1035 TIRDLLSWVEFMQNLATSRHDFSRASIFNACLHGAALIFLDSLEEEERSSDEHMEHEPYLFVDESSLQTSPETLYKFSPVGGINFLLRCLLTHFEVKESNPDLLDDIRTAAQEFVMGLNLQPGQCVPELKDSNKLYGCEPFFIETGPE---LSPKELVGSRAPINFSLSAVTPASNLCRLLRALQLPKRALLLEGSPGVGKTSLVSALARAAGHRVVRINLSEATEASDLFGCDLPVEGAGCGTFAWCDGPLLQALRHGHWVLLDEMNLASQSVLECLNACLDHRGSIYIPELGTTFHVKPRATRLFACQNPVEEGGGRRALPKSFLNRFTQVYLKPLTASDQIAILISIYNDLPVPHIEAMVKFNSMLQDAVERGDSFNAVAGTSWEFNLRDLCRWGDLLIAGQ 1436 HSP 2 Score: 190.274 bits (482), Expect = 2.315e-132 Identity = 144/489 (29.45%), Postives = 243/489 (49.69%), Query Frame = 1
Query: 1150 ASARTKEDQDACGKVYNELADKSRFAR-FIPSGLDFYITDQVLKIGQTQLSVNIANNTDNSKQFNKHLLLAGQLAILESMMLTVDMGWLTVLVGPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGSFEQQKEGGKFKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGLVYGDRIEEIKPHKNFRLFLILDPDC------GGEISKAMRNRGLEICIIPENRELTLTDNDLLAILITIYKLSVKNADSLLRFHKTMI-LAQSETPQIKIGN-------INSLIQAAKLIK---DGYSLDQSLRSVYGDIQMLFNVRDDVNRFINDCNEE------VIDYQEIEQDDFSTCSSLF----NIITEPKKSLF----------LRHVKALKKTLKTQQVVNKNEKLIA-CQLFLQYSTLDDMPNRLEF-VE--FDVDLATVCKKIADAIPKFLNSTNRYLPIDFRWLPNW 2490
A R+ ED+ +++N +A+++ R +IP G + +IG + SVN A T + L+L LE ++ + GW+++L+GP G GK ++V L + I H+ +++++ + DT +LLG+FEQ + GG F W+DS LV+ +R+G W++L+ CS SVLDRLN LLEP G LL++ERGL + +KPH FRL L++D + IS+AMRNRG+E+ + +LT DL +++ LS ++LL F ++I + P++ + + +L+ AA++I+ G LD +L G + ++ + R+ + + + +ID Q D + L N + +L L HV L ++ +VN+ ++ C L S +P + F VE F + +TV + +I +F +S N ID RW+P W
Sbjct: 1453 ARMRSNEDKTRVIELWNGVAEETGLGRCYIPRGCTYPPIGNKFQIGLSTWSVNGAE-TRTEIIEDSLLILNQHRPYLEMLLKILQHGWMSILIGPRGAGKRSLVHLAAQIVHRHVATVALSPSADTVELLGAFEQHENGGIFAWIDSPLVRGIREGHWVVLENAQLCSPSVLDRLNSLLEPGGDLLISERGLDANGELVRLKPHSEFRLILVVDDNTPTVASYSNNISRAMRNRGVEMVL---TNDLTFHKEDLQRLILNT-GLSPACVNALLTFESSVIQRMHTLAPEVTASSLPPRKPPVGALLSAARVIQQITSGKELD-ALMGSEGAEETTEDIAPWMVRWKGEDSRKKAFAKALIDVYSRRQTDHNVSQVLIKAICNFVDNELSTLLVSDQIPLQPRLNHVSELLSSISP--LVNQWRHVLEHCSAVLSSSDNSLLPILIRFSVERGFKIPSSTV----SLSIKQFASSINEVDNIDLRWIPGW 1929 HSP 3 Score: 191.815 bits (486), Expect = 2.292e-44 Identity = 105/229 (45.85%), Postives = 152/229 (66.38%), Query Frame = 1
Query: 9145 WSQYESKTEYLSRQLCEMLRIVLEPTKANKLGGAYRTGKRLNMKKVIPYIASGFRKDKIWLRRNKPSQREYQVIIGVDNSKSMAYNVNHQLFFESLSIVLNGLTFLEVGQVGLCKFGAKAEVVLPLYEKFSSEIGDRLIETFDFSDKCSKFIPILEQSIKLFEENQNFLSSSAVRXXXXXXXXXXXXXXXXXXXXPKFISLIREAHNKGIFVVVILLDHADSKDSILNL 9831
WS Y +++ LS QLCE LR VLEPT+A+++ G +RTGKRLNM+K++PY+AS FRKDKIW+RR +P+QR+Y+++I VDNS SMA N+ + FE+L+ V+N L LE G++G+C FG EVV + E +++E+G ++ FDF + I +L+ SI L + ++S SQLLL+LSDG SED P + +R A + +FVV I++D K I NL
Sbjct: 4597 WSAYVTRSSGLSVQLCEALRQVLEPTRASRMKGDFRTGKRLNMRKIVPYLASQFRKDKIWMRRTQPNQRDYKILIAVDNSSSMADNLCKSMTFEALATVINALNLLEAGKIGVCSFGESVEVVRGMGEPWTNEMGASMLAKFDFKQSRTSLIQLLQTSISLMQVAGEGSATSRGVPASQLLLILSDGVFSEDPQSPALQAAVRLARDHHLFVVCIIIDDNKKKIEIGNL 4825 HSP 4 Score: 117.087 bits (292), Expect = 1.014e-21 Identity = 82/238 (34.45%), Postives = 116/238 (48.74%), Query Frame = 2
Query: 452 SKTTGSNVYRLFRALQVSKK-PLLLEGSPGVGKTSLVTQLAKLVGFKVVRINLSEQTDANDLFG----------CFLPNENGKAGELSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPEINQKFMVEPGKTRLFACQNPVSEGSGRKGLPKSFVNRFTQVYMSSLSDSDYRNILNCLYPAIARETIGFIVEFVQNLREHVVVMKKFGTKGSPWEFNLRDLCRW 1132
++T N+ L R + + P+LL+G VGKTSL+T LA VG RIN E TD G C A L +++G +QA+K G WI+LDE+NLA +LE LN LD E+FI E + P R+FA QNP +GRK L ++ NRF +++ +L ++ IL A+ R +VE + L H++ +G LRDL RW
Sbjct: 460 TETVRGNLKDLARVVSAAGSLPVLLQGETSVGKTSLITFLAARVGQVCHRINNHEHTDLQTYLGAYTAASATSACKNAGSGSGAVPLVFQEGIFVQAMKQGHWIILDELNLAPTEILEALNRVLDENRELFITETQEMVKAHP-HFRVFATQNPPGLYAGRKVLSRALRNRFVELHFDALPRAELEVILEH-RSALPRSRAAKMVEVMHQL--HLIRRSSDIFRGKDGFITLRDLFRW 693 HSP 5 Score: 97.4413 bits (241), Expect = 9.171e-16 Identity = 95/336 (28.27%), Postives = 155/336 (46.13%), Query Frame = 1
Query: 5377 DIYQDPIDVDNLLQAQKILPNANVSVTKLLGIYPEHTTXXXXXXXXXXXXXXSGDYSLIVFINNVERLIHEIQQWEVVAARHVSLIQHVDELNGLLVSWRHQELTMWENTISTANWQLERAVSREFFNHYNLIVQESNKIQYDNDFESINLILEPILNSLLNSTVGNFEYRLKTM-----KLSIESLISNREIDYRFKDRLKNILLNHYDFYRLYLGNVQEHLSSEYKRIGKELRDFIKTKRWGKTPPKIT---DVLKCHKIVFKYKRQWSGIINQ---PCTKFFVLTPINALKQVNLIDIGNCIES--DIDFSKSKLSD----WCATMYEYSREM 6333
D+Y DPI +A ++ V +LL +P+H L+ I IN + S + L +I E L+ E+Q+WE AARHVS+ + LL+ WR EL W ++ T+ + S +F+ ++ +Q ++ D + ++LE + N VG F R + + ++I +S+ +RF R ++ N FY ++ +V++ L E K I KE+ +FI +WG T +V KC K + K+ R W GI+ Q PC + T I + I I+S + DF +LS W + + E REM
Sbjct: 3026 DVYHDPIPKSEGKKAYAMMVAVRARVLQLLQEWPDHPALLKIITVINRMCAFSINDCLTKYITAFEMLLAEMQEWEKNAARHVSISDAFRSVCDLLIEWRKLELKCWMASLDTSTESAANSCSSLWFHLQSVFLQPTSTANIAED-DQCQILLE----FMENGPVGEFAARWRLLAALYNAIAIWPGLSD---THRFSAR--RMVGNVAWFYGQFIPHVKKFLLDEQKPIRKEMSNFISAMKWGDYIGFWTMKENVDKCKKTIHKHIRTWEGILRQSVKPCFDAAIKTSIEMPVPDTTMTILEHIQSIEEKDFGVFRLSPEMKTWLSKLTE-EREM 3350 HSP 6 Score: 96.2857 bits (238), Expect = 1.808e-15 Identity = 64/191 (33.51%), Postives = 105/191 (54.97%), Query Frame = 1
Query: 1417 VLVGPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGSFEQQKEG---GKFKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGLVYGDRIEEIKPHKNFRLFLILDP--DCGGE--ISKAMRNRGLEICIIPENRELTLTDNDLLAILITIYK-LSVKNADSLLRFHKTM 1965
+L G G GKT++V L+ G+ + ++++ A + +DL G + EG G F W D L+QALR G W++LD +N S SVL+ LN L+ GS+ + E G + +KP RLF +P + GG + K+ NR ++ + P LT +D +AILI+IY L V + +++++F+ +
Sbjct: 1223 LLEGSPGVGKTSLVSALARAAGHRVVRINLSEATEASDLFGC-DLPVEGAGCGTFAWCDGPLLQALRHGHWVLLDEMNLASQSVLECLNACLDHRGSIYIPELGTTF-----HVKPRAT-RLFACQNPVEEGGGRRALPKSFLNRFTQVYLKP------LTASDQIAILISIYNDLPVPHIEAMVKFNSML 1400 HSP 7 Score: 82.0333 bits (201), Expect = 3.795e-11 Identity = 60/177 (33.90%), Postives = 88/177 (49.72%), Query Frame = 1
Query: 1417 VLVGPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGSFEQQKEGGK----------FKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGLVYGDRI--EEIKPHKNFRLFLILDPDCGG-----EISKAMRNRGLEI-CIIP----ENRELTLTD 1881
+LVG G GKTTI ++ + + +L ++ + + D LG + F+WVD LV A+ QGD +LD ++ +VL+R+N LLEP + L ER GD + EEI +FRL ++P GG E+S A+RNR EI C P E + +TD
Sbjct: 808 LLVGETGCGKTTICQIFAALRKRNLLCVNCHQYTEAADFLGGLRPIRTHQPDDPNTTDDRLFEWVDGPLVLAMVQGDIFLLDEISLADDAVLERINSLLEPERKICLAER---CGDSLESEEITAVADFRLLATMNP--GGDYAKKELSPALRNRFTEIWCPSPTFIVEKSAIEMTD 979 HSP 8 Score: 72.7886 bits (177), Expect = 2.504e-8 Identity = 66/247 (26.72%), Postives = 110/247 (44.53%), Query Frame = 2
Query: 509 KPLLLEGSPGVGKTSLVTQLAKLVGFKVVRINLSEQTDANDLFGCFLPNENGKAGE--------LSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPE-----INQKFMVEPGKTRLFACQNPVSEGSGRKGLPKSFVNRFTQVY---------MSSLSDSDYRNILNC----------LYPAIARETIGFIVEFVQNLREHV---VVMKKFGTKGSPWEFNLRDLCRWCDLM 1144
+P+LL G G GKT++ A L ++ +N + T+A D G P + + W DGPL+ A+ GD LLDE++LA +VLE +N+ L+ +I + E + + + RL A NP + +K L + NRFT+++ S++ +D++ I+ L P +A+ + F F Q E V + K+ + P +RDL W + M
Sbjct: 805 EPILLVGETGCGKTTICQIFAALRKRNLLCVNCHQYTEAADFLGGLRPIRTHQPDDPNTTDDRLFEWVDGPLVLAMVQGDIFLLDEISLADDAVLERINSLLEPERKICLAERCGDSLESEEITAVADFRLLATMNPGGD-YAKKELSPALRNRFTEIWCPSPTFIVEKSAIEMTDWQAIVEHNLRRSDLLVDLTP-LAKAIVDFCWWFAQGRTEVVCGGMARKRRCRRPFP---TIRDLLSWVEFM 1046 HSP 9 Score: 70.4774 bits (171), Expect = 1.049e-7 Identity = 39/101 (38.61%), Postives = 58/101 (57.43%), Query Frame = 2
Query: 518 LLEGSPGVGKTSLVTQLAKLVGFKVVRINLSEQTDANDLFGCFLPNENGKAGELSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPE 820
+L G G GK SLV A++V V + LS D +L G F +ENG G +W D PL++ ++ G W++L+ L S SVL+ LN+ L+ G++ I E
Sbjct: 1542 ILIGPRGAGKRSLVHLAAQIVHRHVATVALSPSADTVELLGAFEQHENG--GIFAWIDSPLVRGIREGHWVVLENAQLCSPSVLDRLNSLLEPGGDLLISE 1640
BLAST of Midasin vs. Ensembl Cavefish
Match: mdn1 (midasin AAA ATPase 1 [Source:ZFIN;Acc:ZDB-GENE-041001-138]) HSP 1 Score: 320.857 bits (821), Expect = 7.868e-87 Identity = 176/394 (44.67%), Postives = 236/394 (59.90%), Query Frame = 2
Query: 89 VVTIRDLLSWVNFIN--------------ISIG--------NKTSLYQSIFHGAYMVIIDSIND--------------KLNKSFYEELL--IKKLDYLVKSSFPDIVECLDFQSSRFELSVKENKVGIDPFYIELGSHSDWXXXXXXXXXXKNFQYSFNSKTTGSNVYRLFRALQVSKKPLLLEGSPGVGKTSLVTQLAKLVGFKVVRINLSEQTDANDLFGCFLPNENGKAGELSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPEINQKFMVEPGKTRLFACQNPVSEGSGRKGLPKSFVNRFTQVYMSSLSDSDYRNILNCLYPAIARETIGFIVEFVQNLREHVVVMKKFGTKGSPWEFNLRDLCRWCDLMVKHQ 1156
++++RD+LSWV+F+N +S+G + + H A +V +D I K + +F E L I +LD V+ + + E + + R+ E+ GI+PFYI G S+ +S Y+ + TT N R+ RAL++ ++P+LLEGSPGVGKTSLV LAK G ++RINLSEQTD DLFG LP E GK GE +WRDGPLL ALKAG WI+LDE+NLASQSVLE LNAC DHR EI+IPE++ +F V+ KT++F CQNP ++G GRKGLPKSF+NRFTQV++ L+ D + I N ++P I + I +V+F L V V +K+G KG PWEFNLRDL RWC LM Q
Sbjct: 1014 ILSVRDILSWVDFMNTVCEQDEARDENHFMSLGEVEEPEWDQRLDTVTAFIHAACLVYVDGIGSGTTVSAAENASAARKASLAFLESRLRRITELDKKVQDAL-RVYESIQHREPRW----GEDFFGIEPFYIMSGPESEGRSVS---------DYALGAGTTAVNAMRVLRALKL-QRPILLEGSPGVGKTSLVAALAKASGNHLIRINLSEQTDVTDLFGTDLPVEGGKGGEFAWRDGPLLAALKAGHWIVLDELNLASQSVLEGLNACFDHRAEIYIPELSMRFHVQQEKTKIFGCQNPFTQGGGRKGLPKSFLNRFTQVFVDQLTSEDMQFIGNSIFPNINEQIISKMVQFSNKLVHEVTVERKWGQKGGPWEFNLRDLFRWCQLMQADQ 1392 HSP 2 Score: 238.039 bits (606), Expect = 9.255e-62 Identity = 129/284 (45.42%), Postives = 188/284 (66.20%), Query Frame = 1
Query: 9145 WSQYESKTEYLSRQLCEMLRIVLEPTKANKLGGAYRTGKRLNMKKVIPYIASGFRKDKIWLRRNKPSQREYQVIIGVDNSKSMAYNVNHQLFFESLSIVLNGLTFLEVGQVGLCKFGAKAEVVLPLYEKFSSEIGDRLIETFDFSDKCSKFIPILEQSIKLFEENQNFLSSSAVRXXXXXXXXXXXXXXXXXXXXPKFISLIREAHNKGIFVVVILLDHADSKDSILNLKMVIDDG----CDFVRFLDVFPLQNYTVLQDVTKLPTLLTDVLRQWFELSSSADK 9984
W QY++ T LS+QLCE LR++LEPT+A KL G YRTGKRLNM+KVIPYIAS FRKDKIWLRR KPS+R+YQ+ + VD+S SM N + QL FES+S+++N LT LEVGQV +C FG +++ P + +F+ + G R++ F K ++ LE S +F + SA +T+QLLL++SDG + + ++ A + +FV+ ++LD+ +S+DSIL++K+ I G + ++D FP Y +L+DV LP L+D LRQWFEL ++ D+
Sbjct: 4669 WQQYQALTSPLSQQLCEQLRLILEPTQAAKLKGDYRTGKRLNMRKVIPYIASQFRKDKIWLRRTKPSKRDYQICLAVDDSSSMVDNHSKQLAFESVSVIVNALTLLEVGQVAVCSFGESVQLLHPFHHQFNDQSGARILRLCQFQQKKTRIAQFLETSANMFVAAKQQNPGSANTETAQLLLIVSDGRGLFLEGKERVATAVQAARSANVFVIFVVLDNPNSRDSILDIKVPIFKGPGELPEIRSYMDEFPFPFYMILRDVNALPETLSDALRQWFELVTATDQ 4952 HSP 3 Score: 138.272 bits (347), Expect = 1.532e-31 Identity = 78/161 (48.45%), Postives = 99/161 (61.49%), Query Frame = 1
Query: 1516 MDTTDLLGSFEQQKEG---GKFKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGLVYGDRIEEIKPHKNFRLFLILDPDCGGEISKAMRNRGLEICIIPENRELTLTDNDLLAILITIYKLSVKNADSLLRFHKTMILAQSETP 1989
+D T L Q ++G G F+W+D TLVQAL+ GDWL++D VNFCS SVLDRLN LLEP GSL + ERG++ G I PH NFRLFL +DP GEIS+AMRNRG+EI I EN + DL +L T+ D L+ H + A ++P
Sbjct: 1632 LDFTLLKERLAQMEDGWSHGGFEWLDGTLVQALQAGDWLLMDNVNFCSPSVLDRLNALLEPGGSLTINERGVIDGT-TPSITPHPNFRLFLTMDP-AHGEISRAMRNRGVEIYIPGENEGVCWDSLDLKTVLHTLGVTGDCVCDILMSVHSEIKSAIWDSP 1790 HSP 4 Score: 131.724 bits (330), Expect = 1.378e-29 Identity = 84/225 (37.33%), Postives = 119/225 (52.89%), Query Frame = 2
Query: 470 NVYRLFRALQVSKKPLLLEGSPGVGKTSLVTQLAKLVGFKVVRINLSEQTDANDLFGCFLPNENGKAGELSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPEINQKFMVEPGKTRLFACQNPVSEGSGRKGLPKSFVNRFTQVYMSSLSDSDYRNILN--CLYPAIARETIGFIVEFVQNLREHVVVMKKFGTKGSPWEFNLRDLCRWCD 1138
N+ L R + P+L++G VGKTSLV LA G + VRIN E TD + GC+ +E+GK L +++G L+ A++ G WI+LDE+NLA VLE LN LD E+FI E + P + LFA QNP GRK L ++F NRF +++ L + IL+ C P + +++ +Q+LR V G G LRDL RW +
Sbjct: 482 NLRDLARVVAAGIHPVLIQGETSVGKTSLVRWLAAATGNQCVRINNHEHTDIQEYIGCYSSDEHGK---LVFKEGVLIDAMRKGYWIILDELNLAPTDVLEALNRLLDDNRELFIAETQEVVKAHP-RFMLFATQNPPGLYGGRKVLSRAFRNRFVELHFDELPSEELETILHQRCSLPPSYCTKLVKVMQNLQSLRRGSSVFA--GKHGF---ITLRDLFRWAE 697 HSP 5 Score: 93.2041 bits (230), Expect = 6.059e-18 Identity = 71/214 (33.18%), Postives = 105/214 (49.07%), Query Frame = 1
Query: 1252 FYITDQVLKIGQTQLSVNIANNTDNSKQFNKHLLLAG--QLAILESMMLTVDMGWLTVLVGPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGSFEQQKEGGK----------FKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGLVYGDRIEEIKPHKNFRLFLILDPDCGG-----EISKAMRNRGLEI 1842
+ DQV +Q S +A+ ++F + G +LA+L L G +LVG G GKTTI +L + + G S++ + M+T+D LG + G F+W D LV A+RQ ++D ++ SVL+RLN +LE SL+L E+G D +E I K FRL ++P GG E+S A+RNR EI
Sbjct: 753 LFSEDQVT----SQFSPFVASIAGVPEEFGHVVWTEGMRRLAVLVGRALR--FGESVLLVGDTGCGKTTICQLFAALAGQKFFSVNCHLHMETSDFLGGLRPVRHAGTGHGDAEDGRLFEWRDGPLVLAMRQEGVFLMDEISLADDSVLERLNSVLETEKSLVLAEKGSGDDDDVELIIAGKKFRLVATMNP--GGDFGKKELSPALRNRFTEI 958 HSP 6 Score: 89.7373 bits (221), Expect = 6.117e-17 Identity = 100/432 (23.15%), Postives = 192/432 (44.44%), Query Frame = 1
Query: 5371 PHDIYQDPIDVDNLLQAQKILP---NANVSVTKLLGIYPEHTTXXXXXXXXXXXXXXSGDYSLIVFINNVERLIHEIQQWEVVAARHVSLIQHVDELNGLLVSWRHQELTMWENTISTANWQLERAVSREFFNHYNLIVQESNKIQYDNDFESINLILEPILNS---------LLNSTVGNFEYRLKT-MKLSIESLISNREIDYRFKDRLKNILLNHYDFYRLYLGNVQEHLSSEYKRIGKELRDFIKTKRWGKTP--PKITDVLKCHKIVFKYKRQWSGIINQPCTKFFV----------------LTPINALKQV---NLIDIG-------------NCIESDIDFSKSKLSDWCATMYEYS--REMWETMENFQKETKELEDISVVQSKSEDDKEALEKKWRMNVKNLQQRKRNCVSQFVKELREMGL 6519
P+D Y++P N+ QA+ LP + +V + L +PEH L+ I + +L S L F+N +E L+ + Q WE A+R VSL ++ + L++ WR EL W +++ A + ++ +F+ Y LI E + D +S + L+ + S + STVG F RL + L++ ++ L ++L N + +Y + +VQ +S I KEL+DF+K +W V K H+ +FK+ +++ +++PC + TP++ L Q +L G + +++ + K+ C + + + E+ E ++ F E + ++ +QS + D E++ + VK++ +K+ ++ K L ++GL
Sbjct: 3137 PYDFYREP----NMSQARLCLPALEQLSRAVRQRLDDWPEHPALVQITVVMERVLAFSLSSPLAKFLNGLEILLSKAQDWENNASRSVSLRNELESITQLIIQWRKLELNCWSSSLDNAQKRHSENSNKHWFSIYQLI--ERYLQEKRTDSKSADGALDELSLSSVSSTLQGFMEGSTVGEFHTRLAMLLAFHCHVLLAPKQEGQAL---LCSLLWNLHKYYSQFSDSVQSRVSQLRTPIEKELKDFVKISKWNDVSFWSIKQSVEKTHRTLFKFVKKFEAALSEPCAPCLIEQGSSAGMDSVDTQPEETPLHRLHQALNSSLPGRGRDLQDEVMDAVEPSSLQARLPILTKKMRKMCVQLVKTAPLPELSEDLDQFTAEI--ITNVQALQSLTVDRSTEQERQ-KAEVKHILLQKQRALADLFKMLSQIGL 3556 HSP 7 Score: 82.0333 bits (201), Expect = 1.313e-14 Identity = 48/116 (41.38%), Postives = 75/116 (64.66%), Query Frame = 1
Query: 1366 LAILESMMLTVDMGWLTVLVGPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGSFEQQKEGGKFKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTE 1713
L LES+M V MGW+ +LVGP +GK+++V LL+ +TG+ L+ M+MNSAMDTT+LLG FEQ VD +++ +Q +L+++++ A V R GL+ +G++ TE
Sbjct: 1476 LRPLESIMKCVKMGWMAILVGPTASGKSSLVHLLALLTGHRLRVMAMNSAMDTTELLGGFEQ---------VD--IIRPWQQ----VLESMDYVVAMVTRR--GLMSLDGAVQDTE 1574 HSP 8 Score: 80.8777 bits (198), Expect = 2.935e-14 Identity = 54/157 (34.39%), Postives = 80/157 (50.96%), Query Frame = 2
Query: 689 DGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGE--IFIPEINQKFMVEPGKTRLFACQNPVSEGSGRKGLPKSFVNRFTQVYMSSLSD--------SDYRNILNCLYPAIARETIGFIVEFVQNLREHVVVMKKFGTKGSPWEFNLRDLCR 1129
+G L QA+K G+WILLDE+NLA+ LECL+ L+ + + + + +V RLFAC NP ++ G++ LP NRFT++Y+ L SDY LN + R I ++ F +R+ GT P ++LR LCR
Sbjct: 238 EGTLAQAVKKGEWILLDEINLAAPETLECLSGLLEGSAGSLVLLDRGDTEPLVRHSDFRLFACMNPATD-VGKRNLPLGIRNRFTELYVEELESEGDLRILVSDYLKGLN-----LNRSIIHGVISFYLAVRKEANSRLVDGTGHKP-HYSLRTLCR 387 HSP 9 Score: 76.6406 bits (187), Expect = 6.243e-13 Identity = 39/114 (34.21%), Postives = 65/114 (57.02%), Query Frame = 1
Query: 1417 VLVGPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGS--FEQQKEGGKFKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGLVYGDRIEEIK 1752
+L G G GKT++V L+ +GNHL ++++ D TDL G+ + +GG+F W D L+ AL+ G W++LD +N S SVL+ LN + + + E + + + E+ K
Sbjct: 1180 LLEGSPGVGKTSLVAALAKASGNHLIRINLSEQTDVTDLFGTDLPVEGGKGGEFAWRDGPLLAALKAGHWIVLDELNLASQSVLEGLNACFDHRAEIYIPELSMRFHVQQEKTK 1293 HSP 10 Score: 75.0998 bits (183), Expect = 1.918e-12 Identity = 56/182 (30.77%), Postives = 85/182 (46.70%), Query Frame = 2
Query: 488 RALQVSKKPLLLEGSPGVGKTSLVTQLAKLVGFKVVRINLSEQTDANDLFGCFLPNENGKAGE--------LSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPEINQ------KFMVEPGKTRLFACQNPVSEGSGRKGLPKSFVNRFTQVYMSSL-SDSDYRNILN 988
RAL+ + LL+ G G GKT++ A L G K +N + +D G P + G WRDGPL+ A++ L+DE++LA SVLE LN+ L+ + + E + ++ K RL A NP + G+K L + NRFT+++ S SD I+
Sbjct: 795 RALRFGESVLLV-GDTGCGKTTICQLFAALAGQKFFSVNCHLHMETSDFLGGLRPVRHAGTGHGDAEDGRLFEWRDGPLVLAMRQEGVFLMDEISLADDSVLERLNSVLETEKSLVLAEKGSGDDDDVELIIAGKKFRLVATMNPGGD-FGKKELSPALRNRFTEIWCPQTNSRSDLMKIIQ 974 HSP 11 Score: 74.7146 bits (182), Expect = 2.615e-12 Identity = 76/276 (27.54%), Postives = 141/276 (51.09%), Query Frame = 1
Query: 7549 PKLRSLEKVEKEFLGLLLNYHRVLISATDALLTVTTKLMKEGFCIPNDSAWNNEAENSNPSEKMNDFEMAGMDGGVGTNDVTDEIESESQIEGNNKDKKDETENQP---DIPDEDDGIEMTDDFNGKMHD--PEIXXXXXXXXXXXXXXXXXXXMGEVGNAPETDVNXXXXXXXXXXXXXXXXXXXXXXXXGGVSEQNTTEMVADEKEDKDHXXXXXXXXXXXXXXXXXXXXXXTDAGNEEDIGEFKDDEDSKDQENAPENTGQEPEKLDNTEEEN 8361
P L ++ + ++ + + HR L ++ T L ++GFC+P + + E + + +D+E G+ G G DV+D+IE+E Q+E ++ +D+ E +P DI +ED+ IEM++DF+GK HD P E D++ E+ +L+ KMG++G+ P TD E ++ E++++ + +++ G +Q +E+VA + D+ E E +++ E E + E + DE+ D + + EPE +D EE N
Sbjct: 3995 PMLCVYSELVRWYVAVCMGAHRSTGKLLSVLASLFTDLGQKGFCLPQELMGGGDGEGAT---EFHDYEGGGIGEGEGVKDVSDKIENEDQVEDTFREGEDKEEKEPDDRDIKEEDNAIEMSEDFDGKTHDGEPREQGEDEEDSDEEQNEELDKKMGDLGDGP-TDTLDERMWGDEDEDDDDLDGSDKEDESGAGMDQGESELVAKD----DNTDAGESKKDEKNKDKDHPLDKEEKEKIHEQVDEREFDENEVDPYHGKKEQKAEPEAMDLPEELN 4262 HSP 12 Score: 74.3294 bits (181), Expect = 2.750e-12 Identity = 52/161 (32.30%), Postives = 80/161 (49.69%), Query Frame = 1
Query: 1375 LESMMLTVDMGWLTVLV-GPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGSFEQQKEGGKFKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGLVYGDRIEEIKPHKNFRLFLILDPD--CGGE--ISKAMRNRGLEI 1842
L + V G VL+ G GKT++V+ L+ TGN ++ + D + +G + E GK + + L+ A+R+G W+ILD +N VL+ LN LL+ N L + E E +K H F LF +P GG +S+A RNR +E+
Sbjct: 483 LRDLARVVAAGIHPVLIQGETSVGKTSLVRWLAAATGNQCVRINNHEHTDIQEYIGCYSS-DEHGKLVFKEGVLIDAMRKGYWIILDELNLAPTDVLEALNRLLDDNRELFIAETQ-------EVVKAHPRFMLFATQNPPGLYGGRKVLSRAFRNRFVEL 635 HSP 13 Score: 61.6178 bits (148), Expect = 2.040e-8 Identity = 36/97 (37.11%), Postives = 59/97 (60.82%), Query Frame = 1
Query: 1570 FKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPN-GSLLLTERGLVYGDRIEEIKPHKNFRLFLILDP--DCGGE-ISKAMRNRGLEICI 1848
F +V+ TL QA+++G+W++LD +N + L+ L+GLLE + GSL+L +RG E + H +FRLF ++P D G + +RNR E+ +
Sbjct: 234 FAFVEGTLAQAVKKGEWILLDEINLAAPETLECLSGLLEGSAGSLVLLDRG-----DTEPLVRHSDFRLFACMNPATDVGKRNLPLGIRNRFTELYV 325 HSP 14 Score: 55.8398 bits (133), Expect = 1.072e-6 Identity = 35/98 (35.71%), Postives = 50/98 (51.02%), Query Frame = 2
Query: 671 GELSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPEINQKFMVEPGKT-----RLFACQNPVSEGSGRKGLPKSFVNRFTQVYM 949
G W DG L+QAL+AGDW+L+D +N S SVL+ LNA L+ G + I E P T RLF +P + ++ NR ++Y+
Sbjct: 1651 GGFEWLDGTLVQALQAGDWLLMDNVNFCSPSVLDRLNALLEPGGSLTINERGVIDGTTPSITPHPNFRLFLTMDP-----AHGEISRAMRNRGVEIYI 1743 HSP 15 Score: 53.5286 bits (127), Expect = 6.119e-6 Identity = 25/59 (42.37%), Postives = 39/59 (66.10%), Query Frame = 1
Query: 1372 ILESMMLTVDMGWLTVLVGPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGSFE 1548
+LE + + V G +LVG GTGKT+ V+ L+ +TG+ L+ ++MN DT DLLG ++
Sbjct: 77 LLEQLAVCVTKGEPVLLVGETGTGKTSTVQYLAGLTGHKLRVVNMNQQSDTADLLGGYK 135
BLAST of Midasin vs. Ensembl Sea Lamprey
Match: ENSPMAT00000002351.1 (pep scaffold:Pmarinus_7.0:GL479209:15:22864:-1 gene:ENSPMAG00000002142.1 transcript:ENSPMAT00000002351.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 90.8929 bits (224), Expect = 5.073e-18 Identity = 109/449 (24.28%), Postives = 185/449 (41.20%), Query Frame = 1
Query: 5338 SPGVPQNT-IGRPH-DIYQDPIDVDNLLQA-QKILP--NANVSVTKLLGIYPEHTTXXXXXXXXXXXXXXSGDYSLIVFINNVERLIHEIQQWEVVAARHVSLIQHVDELNGLLVSWRHQELTMWENTISTANWQLERAVSREFFNHYNLIVQESNKIQYDNDFESINLILEPILNSLLNSTVGNFEYRLKTMKLSIESLISNREIDYRFKDRLKNILLNHYDFYRLYLGNVQEHLSSEYKRIGKELRDFIKTKRWGKTP--PKITDVLKCHKIVFKYKRQWSGIINQPCTKFFVLTPINALK---------------------------QVNLIDIGNC-------IESDIDFSKSKLSD-----------WCATMYEYSR--EMWETMENFQKE-TKELEDISVVQSKSEDDKEALEKKWRMNVKNLQQRKRNCVSQFVKELREMGL 6519
SPG P R H D Y P N QA Q +LP V V++LL +PEH TL + + IL S L F+ +E L+ + Q WE A+R VSL H+D + L++ +R EL+ W + + + +F+ + L+ E+ D+ S+ L+ + +T+G F RL + LS + ++ L +L N + +Y + +V L+ + K I KEL+DF+K RW V K H+ +F++ R++ +++PC + + P A+ Q LI G C D D + L +C + S ++ + ++ F E + ++ V+ DK+ K R K +QQ+K+ +++ + LR GL
Sbjct: 183 SPGGPDVVESSRAHYDFYHSP----NAAQALQCLLPLKAFTVRVSQLLDEWPEHPTLSQLVIVMTRILGFSLSSPLSKFLYGLELLLAKAQDWESNASRSVSLRPHLDAITQLIIQFRKLELSCWAQCLDNVASRCAEKTNVSWFSLHQLV--ENGVSAEDSPLASVASTLQAFME---GATLGEFPCRLDLL-LSFHCQAVHLPPSAS-REALLGLLWNLHQYYSQFSESVHSALAQKRKAIEKELKDFVKICRWNDASFWAMKEAVNKTHRTLFRFSRKFEEALSEPC-RPALTEPGPAVAADAAQSHDPVAVVTLLRNQRSWVSPSLQAGLIAHGACDSVLQDVAPGDEDSPQESLQGRLPVLSARTWKFCQKLRRESSLPDLIQGLDQFTGEVASSVREMQVLSVNPASDKD----KQRSEAKQIQQQKQRALAELFRLLRRAGL 615 HSP 2 Score: 56.6102 bits (135), Expect = 1.388e-7 Identity = 46/135 (34.07%), Postives = 73/135 (54.07%), Query Frame = 1
Query: 7543 VLPKLRSLEKVEKEFLGLLLNYHRVLISATDALLTVTTKLMKEGFCIPNDSAWNNEAENSNPSEKMNDFEMAGMDGGVGTNDVTDEIESESQIE---GNNKDKKDETENQPDIPDEDDGIEMTDDFNGKMHDPEI 7938
LP L + + +L + HR L LL + T+L+ +GFC P + E E S K D E G+ G G DV+D+IE+E Q+E ++ ++E E Q ++ DE++ IEM++DF GK+H+ E+
Sbjct: 1084 ALPLLGAYADLASFYLVTSVASHRALAKLLCVLLGLFTELVHKGFCQPAEL---TEGEAGEGSGKFQDVEGGGIGAGEGVKDVSDQIENEDQVEDTFQRDQQHEEEPEQQSELNDEENAIEMSEDFQGKLHNGEL 1215
BLAST of Midasin vs. Ensembl Sea Lamprey
Match: ENSPMAT00000007003.1 (pep scaffold:Pmarinus_7.0:GL481838:489617:494557:1 gene:ENSPMAG00000006330.1 transcript:ENSPMAT00000007003.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 65.855 bits (159), Expect = 1.111e-10 Identity = 44/124 (35.48%), Postives = 65/124 (52.42%), Query Frame = 2
Query: 518 LLEGSPGVGKTSLVTQLAKLVGFK----VVRINLSEQTDANDLFGCFLPNENGKAGELSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPEINQKFMVEPGKTRLFACQN 877
L+EG G GKT+LV +LA L G ++ + L +QTD+ L G + GE +WR G L QA+ GDWILL++++ A V+ L L+ G + +P PG +LFA +
Sbjct: 322 LIEGPVGCGKTALVERLAALTGRTAPPGLLTVQLGDQTDSRTLLGMY--RCTDVPGEFTWRAGALTQAVSRGDWILLEDIDYAPLDVISVLIPLLES-GVLSVPGRGDCVRAAPG-FQLFATRR 441 HSP 2 Score: 60.8474 bits (146), Expect = 3.963e-9 Identity = 40/140 (28.57%), Postives = 70/140 (50.00%), Query Frame = 1
Query: 1369 AILESMMLTVDMGWLTVLVGPAGTGKTTIVKLLSDITGNH----LKSMSMNSAMDTTDLLGSFEQQKEGGKFKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGLVYGDRIEEIKPHKNFRLF 1776
A+L ++ V ++ GP G GKT +V+ L+ +TG L ++ + D+ LLG + G+F W L QA+ +GDW++L+ +++ V+ L LLE +G L + RG + ++ F+LF
Sbjct: 306 ALLRALASAVASRRAALIEGPVGCGKTALVERLAALTGRTAPPGLLTVQLGDQTDSRTLLGMYRCTDVPGEFTWRAGALTQAVSRGDWILLEDIDYAPLDVISVLIPLLE-SGVLSVPGRG-------DCVRAAPGFQLF 437
BLAST of Midasin vs. Ensembl Yeast
Match: MDN1 (Huge dynein-related AAA-type ATPase (midasin); forms extended pre-60S particle with the Rix1 complex (Rix1p-Ipi1p-Ipi3p); acts in removal of ribosomal biogenesis factors at successive steps of pre-60S assembly and export from nucleus [Source:SGD;Acc:S000004096]) HSP 1 Score: 275.789 bits (704), Expect = 5.154e-74 Identity = 160/370 (43.24%), Postives = 220/370 (59.46%), Query Frame = 2
Query: 89 VVTIRDLLSWVNFIN---ISIGNKTSLYQSIFHGAYMVIIDSI----------NDKLNKSFYEELLIKKLDYLVKSSFPDIVECLDFQSSRFELSVKENKVGI--DPFYIELGSHSDWXXXXXXXXXXKNFQYSFNSKTTGSNVYRLFRALQVSKKPLLLEGSPGVGKTSLVTQLAKLVGFKVVRINLSEQTDANDLFGCFLPNENGKAGELSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPEINQKFMVEPGKTRLFACQNPVSEGSGRKGLPKSFVNRFTQVYMSSLSDSDYRNILNCLYPAIARETIGFIVEFVQNLREHVVVMKKFGTKGSPWEFNLRDLCRWCDLMVKH 1153
V+++RD+L+WV FIN I NK++ ++ GA MV ID++ N+ KS E +I+ L D +E +++ ++ E +VG+ P + + S S ++ + TT SN+ R+ RA+QV KP+LLEGSPGVGKTSL+T LA + G K+ RINLSEQTD DLFG P E ++GE W D P L+A+K G+W+LLDEMNLASQSVLE LNACLDHRGE +IPE++ F P +FA QNP +G GRKGLPKSFVNRF+ V++ L+ D I LYP+I + I +++ + L + V K +G GSPWEFNLRD RW L+ ++
Sbjct: 1611 VISLRDILAWVEFINKVFPKIQNKST---ALIQGASMVFIDALGTNNTAYLAENENDLKSLRTECIIQLLKLC-----GDDLELQQIETNEIIVTQDELQVGMFKIPRFPDAQSSS----------------FNLTAPTTASNLVRVVRAMQV-HKPILLEGSPGVGKTSLITALANITGNKLTRINLSEQTDLVDLFGADAPGE--RSGEFLWHDAPFLRAMKKGEWVLLDEMNLASQSVLEGLNACLDHRGEAYIPELDISFSCHPN-FLVFAAQNPQYQGGGRKGLPKSFVNRFSVVFIDMLTSDDLLLIAKHLYPSIEPDIIAKMIKLMSTLEDQVCKRKLWGNSGSPWEFNLRDTLRWLKLLNQY 1952 HSP 2 Score: 188.348 bits (477), Expect = 1.466e-47 Identity = 109/288 (37.85%), Postives = 169/288 (58.68%), Query Frame = 1
Query: 9136 RFTWSQYESKTEYLSRQLCEMLRIVLEPTKANKLGGAYRTGKRLNMKKVIPYIASGFRKDKIWLRRNKPSQREYQVIIGVDNSKSMAYNVNHQLFFESLSIVLNGLTFLEVGQVGLCKFGAKAEVVLPLYEKFSSEIGDRLIETFDFSDKCSKFIPILEQSIKLFEENQNFLSSSAVRXXXXXXXXXXXXXXXXXXXXPKFISLIREAHNKGIFVVVILLDHADSKDSILNLKMV--IDD-----GCDFVRFLDVFPLQNYTVLQDVTKLPTLLTDVLRQWF-ELSSS 9975
R W + E T L +L E LR++LEPT A KL G Y+TGKRLNMK++IPYIAS FRKDKIWLRR KPS+R+YQ++I +D+SKSM+ + +L F+SL +V LT LE G + + KFG + V ++FS+E G R + F F + + ++ +S K+FE + + + + + + + + L+R A I +V +++D S +SIL++ V I D ++LD FP + Y V+ D+++LP +L+ +LRQ+F +L+SS
Sbjct: 4630 RELWHKSEISTADLVSRLGEQLRLILEPTLATKLKGDYKTGKRLNMKRIIPYIASQFRKDKIWLRRTKPSKRQYQIMIALDDSKSMSESKCVKLAFDSLCLVSKTLTQLEAGGLSIVKFGENIKEVHSFDQQFSNESGARAFQWFGFQETKTDVKKLVAESTKIFERARAMVHNDQWQLEIVISDGICEDHETIQ-------KLVRRARENKIMLVFVIIDGITSNESILDMSQVNYIPDQYGNPQLKITKYLDTFPFEFYVVVHDISELPEMLSLILRQYFTDLASS 4910 HSP 3 Score: 133.65 bits (335), Expect = 5.025e-31 Identity = 85/225 (37.78%), Postives = 121/225 (53.78%), Query Frame = 2
Query: 470 NVYRLFRALQVSKKPLLLEGSPGVGKTSLVTQLAKLVGFKVVRINLSEQTDANDLFGCFLPNENGKAGELSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPEINQKFMVEPGKTRLFACQNPVSEGSGRKGLPKSFVNRFTQVYMSSLSDSDYRNIL--NC-LYPAIARETIGFIVEFVQNLREHVVVMKKFGTKGSPWEFNLRDLCRWC 1135
N+ L RA + P+L++G GKTS++ LA + G K VRIN E TD + G ++ ++ GK LS+++G L++AL+ G WI+LDE+NLA VLE LN LD E+FIPE + P LFA QNP GRK L ++F NRF +++ + + IL C + P+ A++ IVE + L + F K S LRDL RW
Sbjct: 1064 NMMNLVRATSGKRFPVLIQGPTSSGKTSMIKYLADITGHKFVRINNHEHTDLQEYLGTYVTDDTGK---LSFKEGVLVEALRKGYWIVLDELNLAPTDVLEALNRLLDDNRELFIPETQEVVHPHPD-FLLFATQNPPGIYGGRKILSRAFRNRFLELHFDDIPQDELEIILRERCQIAPSYAKK----IVEVYRQLSIERSASRLFEQKNSF--ATLRDLFRWA 1278 HSP 4 Score: 110.923 bits (276), Expect = 2.970e-24 Identity = 62/121 (51.24%), Postives = 79/121 (65.29%), Query Frame = 1
Query: 1489 LKSMSMNSAMDTTDLLGSFEQQKEGGKFKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGLVYGD-RIEEIKPHKNFRLFLILDPDCGGEISKAMRNRGLEICI 1848
LK+MSMN + + ++ KF+W D LV+A+ +G WLILD N CS SVLDRLN LLE +GSLL+ E G R+ +KPH NFRLFL +DP GE+S+AMRNRG+EI I
Sbjct: 2170 LKTMSMN-----IEKMTEIITKEASVKFEWFDGMLVKAVEKGHWLILDNANLCSPSVLDRLNSLLEIDGSLLINECSQEDGQPRV--LKPHPNFRLFLTMDPKY-GELSRAMRNRGVEIYI 2282 HSP 5 Score: 87.8113 bits (216), Expect = 2.995e-17 Identity = 53/155 (34.19%), Postives = 86/155 (55.48%), Query Frame = 1
Query: 1417 VLVGPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGSFEQQKEGGKFKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGLVYGDRIEEIKPHKNFRLFLILDPD--CGGE--ISKAMRNRGLEICI--IPENR 1863
++ GP +GKT+++K L+DITG+ ++ + D + LG++ + GK + + LV+ALR+G W++LD +N VL+ LN LL+ N L + E E + PH +F LF +P GG +S+A RNR LE+ IP++
Sbjct: 1080 LIQGPTSSGKTSMIKYLADITGHKFVRINNHEHTDLQEYLGTY-VTDDTGKLSFKEGVLVEALRKGYWIVLDELNLAPTDVLEALNRLLDDNRELFIPETQ-------EVVHPHPDFLLFATQNPPGIYGGRKILSRAFRNRFLELHFDDIPQDE 1226 HSP 6 Score: 84.3445 bits (207), Expect = 3.260e-16 Identity = 52/170 (30.59%), Postives = 87/170 (51.18%), Query Frame = 1
Query: 1417 VLVGPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGSFEQQKEGGKFKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGLVYGDRIEEIKPHKNFRLFLILDPDCGG----EISKAMRNRGLEICIIPENRELTLTDNDLLAILITIY 1914
+L G G GKT+++ L++ITGN L ++++ D DL G+ + G+F W D+ ++A+++G+W++LD +N S SVL+ LN L+ G + E + + H NF +F +P G + K+ NR + I LT +DLL I +Y
Sbjct: 1744 LLEGSPGVGKTSLITALANITGNKLTRINLSEQTDLVDLFGADAPGERSGEFLWHDAPFLRAMKKGEWVLLDEMNLASQSVLEGLNACLDHRGEAYIPELDISFS-------CHPNFLVFAAQNPQYQGGGRKGLPKSFVNR-FSVVFID-----MLTSDDLLLIAKHLY 1900 HSP 7 Score: 76.2554 bits (186), Expect = 1.157e-13 Identity = 65/273 (23.81%), Postives = 116/273 (42.49%), Query Frame = 2
Query: 503 SKKPLLLEGSPGVGKTSLVTQLAKLVGFKVVRINLSEQTDAND--------------------------------------LFGCFLPNENGKAGE----------------LSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPE--INQKFMVEPGKTRLFACQNPVSEGSGRKGLPKSFVNRFTQVYMSSLSD-SDYRNILNCLYPAIARETIGFIVEFVQNLREHVVVMKKFGTKGSPWEFNLRDLCRWCDLMVK 1150
+K+P+LL G G GKT++ LA+ +G +++ +N + T+ D L + ++N E W DGPL+QA++ G++ LLDE++LA SVLE LN+ L+ + + E + + + FA NP + G+K L + NRFT++++ S+ D +D I++ ++ IV+F + + K G + +LRD+ W + + K
Sbjct: 1360 NKEPVLLVGETGCGKTTICQLLAQFMGRELITLNAHQNTETGDILGAQRPVRNRSEIQYKLIKSLKTALNIANDQDVDLKELLQLYSKSDNKNIAEDVQLEIQKLRDSLNVLFEWSDGPLIQAMRTGNFFLLDEISLADDSVLERLNSVLEPERSLLLAEQGSSDSLVTASENFQFFATMNPGGD-YGKKELSPALRNRFTEIWVPSMEDFNDVNMIVSSRLLEDLKDLANPIVKFSEWFGK-----KLGGGNATSGVISLRDILAWVEFINK 1626 HSP 8 Score: 75.485 bits (184), Expect = 1.929e-13 Identity = 47/121 (38.84%), Postives = 72/121 (59.50%), Query Frame = 2
Query: 461 TGSNVYRLFRALQVSKKPLLLEGSPGVGKTSLVTQLAKLVGFK--VVRINLSEQTDANDLFGCFLPNENGKAGELSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIP 817
T S++ +L R +Q S P++L G G GKT L+ +L+K +G +V+I+L EQTDA L G + + K G WR G L A+K G W+L+++++ A VL L + L+ R E+ IP
Sbjct: 294 TVSSLRQLGRKIQNST-PIMLIGKAGSGKTFLINELSKYMGCHDSIVKIHLGEQTDAKLLIGTYTSGD--KPGTFEWRAGVLATAVKEGRWVLIEDIDKAPTDVLSILLSLLEKR-ELTIP 410 HSP 9 Score: 72.7886 bits (177), Expect = 1.039e-12 Identity = 40/96 (41.67%), Postives = 60/96 (62.50%), Query Frame = 1
Query: 1570 FKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGLVYGDRIEEIKPHKNFRLFLILDP--DCG-GEISKAMRNRGLEICI 1848
F+W D L+QA+R G++ +LD ++ SVL+RLN +LEP SLLL E+G D + + +NF+ F ++P D G E+S A+RNR EI +
Sbjct: 1472 FEWSDGPLIQAMRTGNFFLLDEISLADDSVLERLNSVLEPERSLLLAEQG--SSDSL--VTASENFQFFATMNPGGDYGKKELSPALRNRFTEIWV 1563 HSP 10 Score: 72.7886 bits (177), Expect = 1.208e-12 Identity = 50/148 (33.78%), Postives = 83/148 (56.08%), Query Frame = 1
Query: 1486 HLKSMSMNSAMDTTDLLGSFEQQKEGGK----FKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLL-EPNG-SLLLTERGLVYGDRIEEIKPHKNFRLFLILDP--DCGG-EISKAMRNRGLEICIIPENRELTLTDNDLLAIL 1902
H K + ++ D D + FE Q + F +V+ +LV+ +R G+WL+LD VN +A L+ ++ LL EP+ S+LL+E+G E IK H +FR+F ++P D G ++ +R+R EI + R++T DLL+I+
Sbjct: 777 HEKKLLLDKWADFNDSVKKFEAQSSSIENSFVFNFVEGSLVKTIRAGEWLLLDEVNLATADTLESISDLLTEPDSRSILLSEKG-----DAEPIKAHPDFRIFACMNPATDVGKRDLPMGIRSRFTEIYVHSPERDIT----DLLSII 915 HSP 11 Score: 65.855 bits (159), Expect = 1.261e-10 Identity = 70/273 (25.64%), Postives = 120/273 (43.96%), Query Frame = 1
Query: 5377 DIYQDPIDVDNLLQAQKILPNANVSVTKLLGIYPEHTTXXXXXXXXXXXXXXSGDYSLIVFINNVERLIHEIQQWEVVAARHVSLIQHVDELNGLLVSWRHQELTMWENTISTANWQLERAVSREFFNHYNLIVQESNKIQYDNDFESINLILEPILNSLLN-STVGNFEYRLKTMKLSIESLISNREIDYRFKDRLKNILLNHYDFYRLYLGNVQEHLSSEYKRIGKELRDFIKTKRWGKTPPKITDVL-----KCHKIVFKYKRQWSGIIN 6177
D Y D + +A I+ SV KLL +PEH TL + + LN L + +E++ + +WE A+ VSL V + L+VSWR EL W+ ++ + + +++ + +F Y IV SN + + +L LN + ST+G F RL +K + + + I R ++ +L N FY + + E +++ K + KE+ D I W D L K H ++K R++ ++N
Sbjct: 3281 DFYHD-FSIPEFQKAGDIIETVLKSVLKLLKQWPEHATLKELYRVSQEFLNYPIKTPLARQLQKIEQIYTYLAEWEKYASSEVSLNNTVKLITDLIVSWRKLELRTWKGLFNSEDAKTRKSIGKWWFYLYESIVI-SNFVSEKKETAPNATLLVSSLNLFFSKSTLGEFNARLDLVKAFYKHI---QLIGLR-SSKIAGLLHNTIKFYYQFKPLIDERITNGKKSLEKEIDDIILLASWKDVN---VDALKQSSRKSHNNLYKIVRKYRDLLN 3544 HSP 12 Score: 64.6994 bits (156), Expect = 3.014e-10 Identity = 38/119 (31.93%), Postives = 70/119 (58.82%), Query Frame = 2
Query: 689 DGPLLQALKAGDWILLDEMNLASQSVLECLNACL---DHRGEIFIPEINQKFMVEPGKT----RLFACQNPVSEGSGRKGLPKSFVNRFTQVYMSSLSDSDYRNILNCLYPAIARETIG 1024
+G L++ ++AG+W+LLDE+NLA+ LE ++ L D R + +++K EP K R+FAC NP ++ G++ LP +RFT++Y+ S + D ++L+ + I + ++
Sbjct: 813 EGSLVKTIRAGEWLLLDEVNLATADTLESISDLLTEPDSRSIL----LSEKGDAEPIKAHPDFRIFACMNPATD-VGKRDLPMGIRSRFTEIYVHS-PERDITDLLSIIDKYIGKYSVS 925 HSP 13 Score: 61.2326 bits (147), Expect = 3.626e-9 Identity = 28/86 (32.56%), Postives = 49/86 (56.98%), Query Frame = 1
Query: 1354 LAGQLAILESMMLTVDMGWLTVLVGPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGSFEQQKEGGKFKWVDSTLVQALRQ 1611
L +A+ ES++ ++ W VLVGP+ +GKT ++ L+ I G + SMNS +D+ D+LG +EQ + ++ L +R+
Sbjct: 2030 LECNVAVYESVLKAINNNWPLVLVGPSNSGKTETIRFLASILGPRVDVFSMNSDIDSMDILGGYEQVDLTRQISYITEELTNIVRE 2115 HSP 14 Score: 55.8398 bits (133), Expect = 1.585e-7 Identity = 34/121 (28.10%), Postives = 59/121 (48.76%), Query Frame = 1
Query: 1417 VLVGPAGTGKTTIVKLLSDITGNH--LKSMSMNSAMDTTDLLGSFEQQKEGGKFKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGLVYGDRIEEIKPHKNFRL 1773
+L+G AG+GKT ++ LS G H + + + D L+G++ + G F+W L A+++G W++++ ++ VL L LLE +R L R E +K F+L
Sbjct: 312 MLIGKAGSGKTFLINELSKYMGCHDSIVKIHLGEQTDAKLLIGTYTSGDKPGTFEWRAGVLATAVKEGRWVLIEDIDKAPTDVLSILLSLLE--------KRELTIPSRGETVKAANGFQL 424 HSP 15 Score: 53.9138 bits (128), Expect = 5.566e-7 Identity = 55/201 (27.36%), Postives = 92/201 (45.77%), Query Frame = 2
Query: 89 VVTIRDLLSWVNFINISIGN----------KTSLYQSIFHGAYMVIIDSINDKLNKSFYEELLIKKLDYLVKSSFPDIVECLDFQSSRFELSVKENKVGIDPFYIELGSHSDWXXXXXXXXXXKNFQYSFNSKTTGSNVYRLFRALQVS---KKPLLLEGSPGVGKTSLVTQLAKLVGFKVVRINLSEQTDANDLFGCFLP 652
VV++RDL+ ++I N ++S+Y SIF A +I + K L+ ++++ I E LD SSR L + ++ ++ + L L+ K+ + + T S RL + V +P+LL G G GKT++V QLAK++ K+ IN+S+QT+ DL G + P
Sbjct: 511 VVSVRDLIKLCERLDILFKNNGINKPDQLIQSSVYDSIFSEAADCFAGAIGE-----------FKALEPIIQA----IGESLDIASSRISLFLTQHVPTLENLDDSIKIGRAVLLKEKLNIQKKSMNSTLFAFTNHS--LRLMEQISVCIQMTEPVLLVGETGTGKTTVVQQLAKMLAKKLTVINVSQQTETGDLLGGYKP 694 HSP 16 Score: 50.8322 bits (120), Expect = 4.480e-6 Identity = 37/120 (30.83%), Postives = 60/120 (50.00%), Query Frame = 2
Query: 683 WRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPEINQK----FMVEPGKT-RLFACQNPVSEGSGRKGLPKSFVNRFTQVYMSSLSDSDYRNILNCLYPAIARETIGF 1027
W DG L++A++ G W++LD NL S SVL+ LN+ L+ G + I E +Q+ +++P RLF +P L ++ NR ++Y+ L+ A R T+GF
Sbjct: 2194 WFDGMLVKAVEKGHWLILDNANLCSPSVLDRLNSLLEIDGSLLINECSQEDGQPRVLKPHPNFRLFLTMDP-----KYGELSRAMRNRGVEIYIDE---------LHSRSTAFDRLTLGF 2299
BLAST of Midasin vs. Ensembl Medaka
Match: mdn1 (midasin AAA ATPase 1 [Source:NCBI gene;Acc:101161112]) HSP 1 Score: 297.745 bits (761), Expect = 7.910e-80 Identity = 180/414 (43.48%), Postives = 237/414 (57.25%), Query Frame = 2
Query: 20 SVAGIIVRFLL-IVQKMSHEINFS---VVTIRDLLSWVNFINI-------------SIGN-----KTSLYQSIFHGAYMVIIDSINDKLNKSFYEELLIKKL------DYLVKSSFPD--IVECLDFQSSRF--ELSVKENKVGIDPFYIELGSHSDWXXXXXXXXXXKNFQYSFNSKTTGSNVYRLFRALQVSKKPLLLEGSPGVGKTSLVTQLAKLVGFKVVRINLSEQTDANDLFGCFLPNENGKAGELSWRDGPLLQALKAGDWILLDE-MNLASQSVLECLNACLDHRGEIFIPEINQKFMVEPGKTRLFACQNPVSEGSGRKGLPKSFVNRFTQVYMSSLSDSDYRNILNCLYPAIARETIGFIVEFVQNLR--EHVVVMKKFGTKGSPWEFNLRDLCRWCDLMVKHQ 1156
S+ G I +L ++ ++H+ +F ++++RD+LSWVNF+N +G+ K + H A +V IDSI LL ++L L K + D +++ L SR E E+ GIDPFYI L + Y+ + TT N + ++P+LLEGSPGVGKTSLV LAK G +VRINLSEQTD DLFG LP E GK GE +WRDGPLL ALKAG W++LDE +NLASQSVLE LNAC DHR EIFIPE+ F V+ KT++F CQNP ++G GRKGLPKSF+NRFTQV++ LS D I + ++P I +E +VEF L+ + V V + +G +GSPWEFNLRD+ RWC LM Q
Sbjct: 1518 SLKGDIAELMLDFIEWLTHQ-DFGRRCILSVRDVLSWVNFLNAVSKRDEDRLMTTEPVGDEGTDLKLDSVTAFIHAACLVYIDSIGSTTASCADNALLARRLCLGFLEQRLSKLTELDQRMLDALRVYDSRLPREPLWGEDFFGIDPFYIALPC----------TDGRDLVDYAITAGTTAVNA-QRLLRALKLRRPVLLEGSPGVGKTSLVAALAKASGNHLVRINLSEQTDVTDLFGTDLPVEGGKGGEFAWRDGPLLAALKAGHWVVLDEQLNLASQSVLEGLNACFDHRAEIFIPELGLSFQVQHEKTKIFGCQNPFTQGGGRKGLPKSFLNRFTQVFVDQLSSEDMEFIADSIFPNIDKEIRVKMVEFSNKLQLVQEVCVQRHWGQRGSPWEFNLRDMFRWCQLMQNDQ 1919 HSP 2 Score: 225.328 bits (573), Expect = 6.148e-58 Identity = 167/476 (35.08%), Postives = 250/476 (52.52%), Query Frame = 1
Query: 8725 ESSDKKQDVSSKP---SENRSLGDVNQTFKRKAEILDENNADDNKRPRTTDAFSHIDPENPVESTEQILDD----ATAEQQQHXXXXXXXXXXXXXXXYAIENEK----------------------------NAELETNNLINEVD---HNDNKKSENRERTXXXXXXXXXXXXRF-----------TWSQYESKTEYLSRQLCEMLRIVLEPTKANKLGGAYRTGKRLNMKKVIPYIASGFRKDKIWLRRNKPSQREYQVIIGVDNSKSMAYNVNHQLFFESLSIVLNGLTFLEVGQVGLCKFGAKAEVVLPLYEKFSSEIGDRLIETFDFSDKCSKFIPILEQSIKLFEENQNFLSSSAVRXXXXXXXXXXXXXXXXXXXXPKFISLIREAHNKGIFVVVILLDHADSK----DSILNLKMVIDDG----CDFVRFLDVFPLQNYTVLQDVTKLPTLLTDVLRQWFELSSSAD 9981
++ K Q KP E RS GD N+ ++ ++ ++ + +KR + +D D V E D A+ EQQ+ + +E+ A+E E+ + E+E E + D ++ + ERT+ + + W +Y++ T LS+QLCE LR++LEPT+A KLG YRTGKRLNM+KVIPYIAS FRKDKIWLRR KPS+REYQ+ + VD+S SM N + QL FESLS+++N LT LEVGQV +C FG + +++ P ++F+ E G R++ F K ++ LE S K+F + + S+ T+QLLL++SDG + + +R A N IFV+ ++LD +S+ DSIL++K+ I G + ++D FP Y +L+DV LP L+D LRQWFEL ++AD
Sbjct: 4700 QTQKKTQSFKRKPGQADEERSTGDYNERVNKRLRMV-ASSEERSKRNKPSDGKQESDLHEHVREGEMDHDAQTYVASLEQQKAMGLQKNLNEDQEDEDVAMETEQPEEELKAAEVQELKPEQLDSSRSSQRADSGEMEVQRQGEEEEDERQKDRQQVGDEERTQRSSESTVHTVPELLMEATQSQAACMWHRYQNLTAALSQQLCEQLRLILEPTQAAKLGD-YRTGKRLNMRKVIPYIASQFRKDKIWLRRTKPSKREYQICLAVDDSSSMVDNHSKQLAFESLSVIVNALTLLEVGQVSVC-FGEQMQLLHPFQQQFNDESGARILRLCQFQQKKTRIAQFLETSTKMFLSARQQIPGSS-SSTAQLLLIVSDGRGLFLEGKERVTAAVRAARNANIFVIFVVLDDPNSRSVLQDSILDIKVPIFKGPGELPEIRSYMDEFPFPFYVILRDVNALPETLSDALRQWFELVTAAD 5171 HSP 3 Score: 122.479 bits (306), Expect = 8.799e-27 Identity = 85/211 (40.28%), Postives = 120/211 (56.87%), Query Frame = 1
Query: 1423 VGPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGS-FEQQKEG---GKFKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGLVYGDRIEEIKPHKNFRLFLILDPDCGGEISKAMRNRGLEICIIPENRELTLTDN-DLLAILITIYKLSVKNADSLLRFHKTMILAQSETPQIKIGNINSLIQAAKLI 2040
VG K ++ +L + LK +M+ LL QQ++G G F+W+D LVQAL+ GDWL++D NFC ASVLDRLN LLEPNGSL ++ERG++ G + +I PH +F LFL +DP GE+S+AMRNRG+E+ IPE E D D+ +L T + L+ HK + + +P + SL+ AA L+
Sbjct: 2117 VGSEDLNKLEVIIVLLQKLNSKLKVFAMSKLERNFTLLKERMAQQEDGWTNGGFEWLDGMLVQALQTGDWLLMDNANFC-ASVLDRLNALLEPNGSLTVSERGVIDG-KTPKISPHPDF-LFLTMDP-VHGELSRAMRNRGVEL-FIPEEHEDVFWDTLDMKTLLHTAGVTGKCVCNLLIEIHKG-VKSAIWSP---ASSTTSLLHAAALL 2318 HSP 4 Score: 118.627 bits (296), Expect = 1.317e-25 Identity = 79/226 (34.96%), Postives = 116/226 (51.33%), Query Frame = 2
Query: 470 NVYRLFRALQVSKKPLLLEGSPGVGKTSLVTQLAKLVGFKVVRINLSEQTDANDLFGCFLPNENGKAGELSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPEINQKFMVEPGKTRLFACQNPVSEGSGRKGLPKSFVNRFTQVYMSSLSDSDYRNILN---CLYPAIARETIGFIVEFVQNLREHVVVMKKFGTKGSPWEFNLRDLCRWCD 1138
N+ L R + + P+L++G VGKTSL+ LA G + VRIN E TD + GC+ + GK L +++ L+ A++ G WI+LDE+NLA VLE LN LD E+F+ E + P + LFA QNP GRK L ++F NRF +++ L + +IL+ L P+ + + +++ R V K G LRDL RW D
Sbjct: 1029 NLRDLARVVSATH-PVLIQGETSVGKTSLIRFLAAATGNQCVRINNHEHTDIQEYIGCYSSDSRGK---LVFKE-VLIDAMRKGYWIILDELNLAPTDVLEALNRLLDDNRELFVAETQEVVKAHP-RFMLFATQNPPGLYGGRKVLSRAFRNRFVELHFDELPSGELESILHQRCSLPPSYCSKLVKVMLDLQSLRRGSSVFAGKHGF------ITLRDLFRWAD 1242 HSP 5 Score: 88.5817 bits (218), Expect = 1.520e-16 Identity = 39/62 (62.90%), Postives = 52/62 (83.87%), Query Frame = 1
Query: 1366 LAILESMMLTVDMGWLTVLVGPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGSFEQ 1551
L LES+M V+MGW+ VLVGP G+GKT++V+LL+ +TG HL+ M+MNS+MDTT+LLG FEQ
Sbjct: 1994 LRPLESLMKCVEMGWMAVLVGPTGSGKTSLVRLLALLTGRHLRVMAMNSSMDTTELLGGFEQ 2055 HSP 6 Score: 81.6481 bits (200), Expect = 1.770e-14 Identity = 64/189 (33.86%), Postives = 97/189 (51.32%), Query Frame = 1
Query: 1345 HLLLAG---QLAILESMMLTVDMGWLTVLVGPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGSFEQQKEGGK--------FKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGLVYGDRIEEIKPHKNFRLFLILDPDCGG-----EISKAMRNRGLEICIIPENR 1863
H++ G +LA+L L G +LVG GKTTI ++ + + G L S++ + M+T+D LG + + F+W D LV AL++G ++D ++ SVL+RLN +LE L+L E+G D +E IK + FRL ++P GG E+S A+RNR EI NR
Sbjct: 1319 HVVWTGGMRRLAVLVGRALR--FGESVLLVGET-CGKTTICQMFAALAGQTLYSVNCHLHMETSDFLGGLRPVRHSHQTDADDSRLFQWHDGPLVIALKEGGVFLMDEISLADDSVLERLN-VLETEKCLVLAEKGSGDDDDVELIKADQAFRLVATMNP--GGDFGKKELSPALRNRFTEIWCPQSNR 1501 HSP 7 Score: 76.2554 bits (186), Expect = 8.847e-13 Identity = 41/116 (35.34%), Postives = 66/116 (56.90%), Query Frame = 1
Query: 1414 TVLVGPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGS--FEQQKEGGKFKWVDSTLVQALRQGDWLILD-TVNFCSASVLDRLNGLLEPNGSLLLTERGLVYGDRIEEIK 1752
+L G G GKT++V L+ +GNHL ++++ D TDL G+ + +GG+F W D L+ AL+ G W++LD +N S SVL+ LN + + + E GL + + E+ K
Sbjct: 1703 VLLEGSPGVGKTSLVAALAKASGNHLVRINLSEQTDVTDLFGTDLPVEGGKGGEFAWRDGPLLAALKAGHWVVLDEQLNLASQSVLEGLNACFDHRAEIFIPELGLSFQVQHEKTK 1818 HSP 8 Score: 73.1738 bits (178), Expect = 6.440e-12 Identity = 53/188 (28.19%), Postives = 98/188 (52.13%), Query Frame = 2
Query: 458 TTGSNVYRLFRALQVSKKPLLLEGSPGVGKTSLVTQLAKLVGF----KVVRINLSEQTDANDLFGCFLPNENGKAGELSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPEINQKFMVEPGKTRLFACQNPVSEGSGRKGLPKSFVNRFTQVYMSSLSDSDYRNILNCLYPAIA 1009
+T ++ RL A+ S+KP+LLEG G GKT+LV LA G +++++ L +QTD+ L G + + G+ W+ G L QA+ G WILL++++ A V+ L ++ + ++ IP + V PG + FA ++ S + +T++ M +++ + + +L YP ++
Sbjct: 291 STCRDLRRLALAV-ASQKPVLLEGPIGCGKTALVEFLAAATGHWKASEIMKVQLGDQTDSKMLLGMYRCTD--VPGKFVWQPGTLTQAVAKGQWILLEDIDHAPLDVVSVLLPLMESK-KLLIPGRDDCIDVAPG-FQFFATRSGGSWHRPQNSHAALLDKYWTKLQMGNMTREELKKVLVSRYPGLS 473 HSP 9 Score: 68.9366 bits (167), Expect = 1.347e-10 Identity = 73/286 (25.52%), Postives = 139/286 (48.60%), Query Frame = 1
Query: 5371 PHDIYQDPIDVDNLLQAQKILP---NANVSVTKLLGIYPEHTTXXXXXXXXXXXXXXSGDYSLIVFINNVERLIHEIQQ-WEVVAARHVSLIQHVDELNGLLVSWRHQELTMWENTISTANWQLERAVSREFFNHYNLIVQESNKIQYDNDFESINLILEPILNSLLNSTVGNFEYRLKTMKLSIES---LISNREIDYRFKDRLKNILLNHYDFYRLYLGNVQEHLSSEYKRIGKELRDFIKTKRWGKTP--PKITDVLKCHKIVFKYKRQWSGIINQPCTKFFV 6201
P+D YQ P N+ +A+ LP + +V + L +PEH L+ + + S D L F+N +E L+ + QQ WE A+R +SL + ++ + L++ WR EL W +++ A + + S+ +F Y L+ + + + +++ S++ + + ST+G+F RL+ M L+ L+S++ L +L N + +Y + ++Q ++ + I KELRDF+K +W V K H + FK+ +++ ++ P V
Sbjct: 3583 PYDFYQQP----NMSEARLCLPVLEQLSQAVKQRLRDWPEHPALLMVVMERILTF--SLDSPLAKFLNGLEILLSKAQQDWENNASRALSLRKELEAVTQLIIQWRKLELC-WSSSLDNAMIRHTQKSSKHWFAIYQLVERYLEEQRTEHERLSLSSASSTLQAFIEGSTLGDFHTRLQ-MLLNFHCHLLLVSDQP-----GRSLLALLWNLHRYYSQFSESIQARITQLRQPIEKELRDFVKISKWNDVSFWSIKQSVDKTHTL-FKFVKKFEEALSSPSVPALV 3854 HSP 10 Score: 68.1662 bits (165), Expect = 2.030e-10 Identity = 47/148 (31.76%), Postives = 74/148 (50.00%), Query Frame = 1
Query: 1414 TVLVGPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGSFEQQKEGG-KFKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGLVYGDRIEEIKPHKNFRLFLILDPD--CGGE--ISKAMRNRGLEI 1842
++ G GKT++++ L+ TGN ++ + D + +G + G FK V L+ A+R+G W+ILD +N VL+ LN LL+ N L + E E +K H F LF +P GG +S+A RNR +E+
Sbjct: 1043 VLIQGETSVGKTSLIRFLAAATGNQCVRINNHEHTDIQEYIGCYSSDSRGKLVFKEV---LIDAMRKGYWIILDELNLAPTDVLEALNRLLDDNRELFVAETQ-------EVVKAHPRFMLFATQNPPGLYGGRKVLSRAFRNRFVEL 1180 HSP 11 Score: 66.6254 bits (161), Expect = 7.070e-10 Identity = 53/156 (33.97%), Postives = 75/156 (48.08%), Query Frame = 2
Query: 689 DGPLLQALKAGDWILLDEMNLASQSVLECLNACLD-HRGEIFIPEINQKFMVEPGKTRLFACQNPVSEGSGRKGLPKSFVNRFTQVYMSSLSD--------SDYRNILNCLYPAIARETIGFIVEFVQNLREHVVVMKKFGTKGSPWEFNLRDLCR 1129
+G L QA+K G WILLDE+NLA+ LECL+ L+ G + + + +V RLF C NP ++ G++ LP N T+ Y+ L + SDY LN + G I F N+R+ GT P ++LR LCR
Sbjct: 787 EGTLAQAVKKGHWILLDEINLAAAETLECLSGLLEGSAGSLVLLDRGDTPLVRHPDFRLFGCMNPATD-VGKRNLPLGLRN-LTEFYVEELENEGDLRILVSDYLKGLNPPKNVVT----GVI--FYLNVRKEANSSLVDGTGHRP-HYSLRTLCR 933 HSP 12 Score: 63.5438 bits (153), Expect = 5.018e-9 Identity = 73/257 (28.40%), Postives = 117/257 (45.53%), Query Frame = 2
Query: 488 RALQVSKKPLLLEGSPGVGKTSLVTQLAKLVGFKVVRINLSEQTDANDLFGCFLPNENGKAGE------LSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNA-------CLDHRG-----EIFIPEINQKFMVEPGKTRLFACQNPVSEGSGRKGLPKSFVNRFTQVYM-SSLSDSDYRNILNC-LYPAIARETIGFIVEFVQNLREHVVVMKKFGTKGSPWEFNLRDLCRWCDLM--VKHQHEPR-KTRTPVG 1189
RAL+ + LL+ + GKT++ A L G + +N + +D G P + + W DGPL+ ALK G L+DE++LA SVLE LN L +G ++ + + +Q F RL A NP + G+K L + NRFT+++ S SD I+N L+ ++ + G I E + + E + + FG + ++RD+ W + + V + E R T PVG
Sbjct: 1335 RALRFGESVLLVGET--CGKTTICQMFAALAGQTLYSVNCHLHMETSDFLGGLRPVRHSHQTDADDSRLFQWHDGPLVIALKEGGVFLMDEISLADDSVLERLNVLETEKCLVLAEKGSGDDDDVELIKADQAF-------RLVATMNPGGD-FGKKELSPALRNRFTEIWCPQSNRRSDLVQIINHNLHSGLSLK--GDIAELMLDFIE-WLTHQDFGRRCI---LSVRDVLSWVNFLNAVSKRDEDRLMTTEPVG 1575 HSP 13 Score: 59.6918 bits (143), Expect = 8.160e-8 Identity = 39/118 (33.05%), Postives = 71/118 (60.17%), Query Frame = 1
Query: 7579 KEFLGLLLNYHRVLISATDALLTVTTKLMKEGFCIPNDSAWNNEAENSNPSEKMNDFEMAGMDGGVGTNDVTDEIESESQIEGNN--KDKKDETENQPDIPDEDDGIEMTDDFNGKMH 7926
+ +L +L+ HR L ++ T+L ++GFC+P + + E + + +D+E G+ G GT DV+D+IE+E Q+E + +K+E + + DI E++ IEM++DF+G+MH
Sbjct: 4308 RYYLTVLVGVHRSTGKLLSVLASIFTELAQKGFCLPQELMPGGDGEGAT---EFHDYEGGGIGEGEGTKDVSDKIENEDQVEDTSQEGQEKEEQQEKGDIKSEENAIEMSEDFDGQMH 4422 HSP 14 Score: 56.9954 bits (136), Expect = 5.554e-7 Identity = 41/120 (34.17%), Postives = 60/120 (50.00%), Query Frame = 1
Query: 1342 KHLLLAGQLAI-LESMMLTVDMGWLTVLVGPAGTGKTTIVKLLSDITGNHLKSMSMNSAM-DTTD---LLGSFEQQKEGGKFKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLE 1686
K+L+L G L + L V +L GP G GKT +V+ L+ TG+ S M + D TD LLG + GKF W TL QA+ +G W++L+ ++ V+ L L+E
Sbjct: 284 KNLVLVGSTCRDLRRLALAVASQKPVLLEGPIGCGKTALVEFLAAATGHWKASEIMKVQLGDQTDSKMLLGMYRCTDVPGKFVWQPGTLTQAVAKGQWILLEDIDHAPLDVVSVLLPLME 403 HSP 15 Score: 56.6102 bits (135), Expect = 6.791e-7 Identity = 31/75 (41.33%), Postives = 48/75 (64.00%), Query Frame = 1
Query: 1570 FKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPN-GSLLLTERGLVYGDRIEEIKPHKNFRLFLILDP 1791
F +V+ TL QA+++G W++LD +N +A L+ L+GLLE + GSL+L +RG R H +FRLF ++P
Sbjct: 783 FAFVEGTLAQAVKKGHWILLDEINLAAAETLECLSGLLEGSAGSLVLLDRGDTPLVR------HPDFRLFGCMNP 851
BLAST of Midasin vs. Planmine SMEST
Match: SMESG000033712.1 (SMESG000033712.1) HSP 1 Score: 4868.91 bits (12628), Expect = 0.000e+0 Identity = 2608/2635 (98.98%), Postives = 2615/2635 (99.24%), Query Frame = 1
Query: 2095 MLFNVRDDVNRFINDCNEEVIDYQEIEQDDFSTCSSLFNIITEPKKSLFLRHVKALKKTLKTQQVVNKNEKLIACQLFLQYSTLDDMPNRLEFVEFDVDLATVCKKIADAIPKFLNSTNRYLPIDFRWLPNWKEFQLTDFQEINRFLFSIYNIENHLLELKSMEILGNTRPSELTQKAIKKFNVAQLSKLVNTGKLSQNHLSNLVGFDKSIGQFIDQFLSIDISSNFCGMDNFFEMRNVCKLFKRFIEYYPSESFHWRSTCVYHLTSIFNCLTKKQNSGFNDLVKICGQILDKINKIDLSRCLEFVENDEIKDAGIKRNDLRINYPHSPLPVIESQVENKLIIGSEKSKSVWKPSGEEELKKGLCKTFFSLSHIEEPQNMNINELVNHFDVETVRIWPFTLNLLSHILNSCTRPSSIDPIQKFMSGNSEIKNFLPPLPCNFQEKLPEFSDVEKFSTKFTSLADWSNYIIRQGHLIKVIXXXXXXXXXXXXXXQLVLDSSTSYLQSQINKIWRNPDCAVFLLVNGRRCHNDTIGYFNSVCTTNSTKRCIRSIETRKSQLEFSKQIYIECASILTSEQIRIARELKLLKELSNIQRHCIEACWNGLGCLEEWLKTRFPWFLSLENDIFNLTPTGSSDMMLIIGKLWIRWGSVQMKLFALMESLDPQSQLFWESKYLNEEFDQVSKEKQLGNLYREYLTGVKQNNITERCWSEHGLQHPLLHSMSMRIDQLQSSLAKLTENVGLRPCGDEKYNNLRLKTLRYLSKYGWNALCNESMESLLEFDSTFVAEWSLASSEMLDFFFQQENSSMFPDLIEPLILGISRVLVGIEIINSKTIKQNEKAESASKICEIFCRFPDNNINEMVERILNEETSILRCCQVLIGDCTPSMLAKXXXXXXXXXXXXXNFSQSNKTEKLALLHRLLSVFGNLWXXXXXXXXXXXXXXSSMYVEKNKLTENLTEEQIEDLEIFMNVCPSDQQRFDDQHMQXXXXXXXXXXKLQELQQRLTSISSGLTLDEDTIHSVVNLTTRFLNSSHVEEKQDTTVFRDCYRFAAALISSDNQGIPSDDCKLFAANLSALNWEYETLSPGVPQNTIGRPHDIYQDPIDVDNLLQAQKILPNANVSVTKLLGIYPEHTTXXXXXXXXXXXXXXSGDYSLIVFINNVERLIHEIQQWEVVAARHVSLIQHVDELNGLLVSWRHQELTMWENTISTANWQLERAVSREFFNHYNLIVQESNKIQYDNDFESINLILEPILNSLLNSTVGNFEYRLKTMKLSIESLISNREIDYRFKDRLKNILLNHYDFYRLYLGNVQEHLSSEYKRIGKELRDFIKTKRWGKTPPKITDVLKCHKIVFKYKRQWSGIINQPCTKFFVLTPINALKQVNLIDIGNCIESDIDFSKSKLSDWCATMYEYSREMWETMENFQKETKELEDISVVQSKSEDDKEALEKKWRMNVKNLQQRKRNCVSQFVKELREMGLLAHGSDLPRNTALMTHLPLGDIENSQLPFSTIRCLRLRLEVDDSDKSEEFAKFNDFKNRLISICDHLLSIACTNTTKIKHFQNEFNIIGNSIEYLSNTCQSEIIAIQDPTDIERNFSLMTSNNSEKINQWDLFIEILKGATCSRRFPSLLTDDLKPSLLTSFDDRDFLKSLTKQICQVNVPKSYLVNMKHMECLSQQKLIIKGINELIEIVKNRFSPENKKINSSITESLLKFNDSNERAIIEIETSLHVANDNFMDTDDSFDGFVNELAVVIREKISAFKTILLTDNVDKSFIKVNTHINALEQSLDTQKILDLFTKIKSFFCDLNMNQVNKFRNLINEMVLPKLRSLEKVEKEFLGLLLNYHRVLISATDALLTVTTKLMKEGFCIPNDSAWNNEAENSNPSEKMNDFEMAGMDGGVGTNDVTDEIESESQIEGNNKDK-KDETENQPDIPDEDDGIEMTDDFNGKMHDPEIXXXXXXXXXXXXXXXXXXXMGEVGNAPETDVNXXXXXXXXXXXXXXXXXXXXXXXXGGVSEQNTTEMVADEKEDKDHXXXXXXXXXXXXXXXXXXXXXXTDAGNEEDIGEFKDDEDSKDQENAPENTGQEPEKLDNTEEENNPLDDFWNDECQLNVESDSDQVENNDIPDVEEKEENIDDLQPIDETPEDKEEVISGKVEQEFNMAGGYEINENHEMTLDNENVEENTEIXXXXXXXXXXXXXXXXXAEGNIDNNEPKFDNKESSDKKQDVSSKPSENRSLGDVNQTFKRKAEILDENNADDNKRPRTTDAFSHIDPENPVESTEQILDDATAEQQQHXXXXXXXXXXXXXXXYAIENEKNAELETNNLINEVDHNDNKKSENRERTXXXXXXXXXXXXRFTWSQYESKTEYLSRQLCEMLRIVLEPTKANKLGGAYRTGKRLNMKKVIPYIASGFRKDKIWLRRNKPSQREYQVIIGVDNSKSMAYNVNHQLFFESLSIVLNGLTFLEVGQVGLCKFGAKAEVVLPLYEKFSSEIGDRLIETFDFSDKCSKFIPILEQSIKLFEENQNFLSSSAVRXXXXXXXXXXXXXXXXXXXXPKFISLIREAHNKGIFVVVILLDHADSKDSILNLKMVIDDGCDFVRFLDVFPLQNYTVLQDVTKLPTLLTDVLRQWFELSSSADKGPGR 9996
MLFNVRDDVNRFINDCNEEVIDYQEIEQDDFSTCSSLFNIITEPKKSLFLRHVKALKKTLKTQQVVNKNEKLIACQLFLQYSTLDDMPNRLEFVEFDVDLATVCKKIADAIPKFLNSTNRYLPIDFRWLPNWKEFQL DFQEINRFLFSIYNIENHLLELKSMEIL NTRPSELTQKAIKKFNVAQLSKLVNTGKLSQNHLSNLVGFDKSIGQFIDQFLSIDISSNFCGMDNFFEMRNVCKLFKRFIEYYPSESFHWRSTCVYHLTSIFNCLTKKQNS FNDLVKIC QILDKINKIDLSRCLEFVENDEIKDAGIKRNDLRINYPHSPLPVIESQVEN+LIIGSEKSKSVWKPSGEEELKKGLCKTFFSLSHIEEPQNMNINELVNHFDVETVRIWPFTLNLLSHILNSCTRPSSIDPIQKFMSGNSEIKNFLPPLPCNFQEKLPEFSDVEKFSTKFTSLADWSNYIIRQGHLIKVISSCSYYFDSDSDEYQLVLDSSTSYLQSQINKIWRNPDCAVFLLVNGRRCHNDTIGYFNSVCTTNSTKRCIRSIETRKSQLEFSKQIYIECASILTSEQIRIARELKLLKELSNIQRHCIEACWNGLGCLEEWLKTRFPWFLSLENDIFNL PT SSDMMLIIGKLWIRWGSVQMKLFALMESLDPQSQLFWESKYLNEEFDQVSKEKQLGNLYREYLTGVKQNNITERCWSEHGLQHPLLHSMSMRIDQLQSSLAKLTENVGLRPCGDEKYNNLRLKTLRYLSKYGWNALCNESMESLLEFDSTFVAEWSLASSEMLDFFFQQENSSMFPDLIEPLILGISRVLVGIEIINSKTIKQNEK+ESASKICEIFCRFPDNNINEMVERILNEETSILRCCQVLIGDCTPSMLAKIHIRLLHLCLRLLNFSQSNKTEKLALLHRLLSVFGNLWAKQQQKQLEKEEAESSMYVEKNKLTENLTEEQIEDLEIFMNVCPSDQQRFDDQHMQMSASMSSAAMKLQE+QQRLTSISSGLTLDEDTIHSVVNLTT FLNSSHVEEKQDTTVFRDCYRFAAALIS+DNQGIPSDDCKLFAAN SALNWEYETLSPGVPQNTIGRPHDIYQDPIDVDNLLQAQKILPNANVSVTKLLGIYPEHTTLINIQKKINNILNLSGDYSLIVFINNVERLIHEIQQWEVVAARHVSLIQHVDELNGLLVSWRHQELTMWENTISTANWQLERAVSREFFNHYNLIVQESNKIQYDNDFESINLILEPILNSLLNSTVGNFEYRLKTMKLSIESLISNREIDYRFKDRLKNILLNHYDFYRLYLGNVQEHLSSEYKRIGKELRDFIKTKRWGKTPPKITDVLKCHKIVFKYKRQWSGIINQPCTKFFVLTPINALKQVNLIDIGNCIESDIDFSKSKLSDWCATM EYSREMWETMENFQKETKELEDISVVQSKSEDDKEALEKKWRMNVKNLQQRKRNCVSQFVKELREMGLLAHGSDLPRNTALMTHLPLGDIENSQLPFSTIRCLRLRLEVDDSDKSE+FAKFNDFKNRLISICDHLLSIACTNTTKIKHFQNEFNIIGNSIEYLSNTCQSEIIAIQDPTDIERNFSLMTSNNSE+INQWDLFIEILKGATCSRRFPSLLTDDLKPSLLTSFDDRDFLKSLTKQICQVNVPKSYLVNMKHMECLSQQK IIKGINELIEIVKNRFSPENKKINSSITESLLKFNDSNERAIIEIETSLHVANDNFMDTDDSFDGFVNELAVVIR+KISAFKTILLTDNVDKSFIKVNTHINALEQSLDTQKILDLFTKIKSFFCDLNMNQVNKFRNLINEMVLPKLRSLEKVEKEFLGLLLNYHRVLISATDALLTVTTKLMKEGFCIPNDSAWNNEAENSNPSEKMNDFEMAGMDGGVGTNDVTDEIESESQIEGNNKDK KDETENQPDIPDEDDGIEMTDDFNGKMHDPEIDESKENDNNNEELNDLEDKMGEVGNAPETDVNKEAWDNEKPEEENEKEDKNEDKRKGGVSEQNTTEMVADE EDKD NSKSEGNNKENSEELKEEESTETDAGNEEDIGEFKDDEDSKDQ ENTGQEPEKLDN EEENNPLDDFWNDECQLNVESDSDQVENN IPDVEEKEENIDDLQPIDETPEDKEEVISGKVEQEFNMAGGYEINENHEMTLDNENVEENTEIGATGNGSGSSNSMNTENAEGNIDNNEPKFDNKESSDKKQDVSSKPSENRSLGDVNQTFKRKAEILDENNADDNKRPRTTDAFSHIDPENPVESTEQILDDATAEQQQHIPEINEEENVTIEENYAIENEKNAELETNNLINEVD NDNKKSENRERTEIIIQPEEKKIERFTWSQYESKTEYLSRQLCEMLRIVLEPTKANKLGGAYRTGKRLNMKKVIPYIASGFRKDKIWLRRNKPSQREYQVIIGVDNSKSMAYNVNHQLFFESLSIVLNGLTFLEVGQVGLCKFGAKAEVVLPLYEKFSSEIGDRLIETFDFSDKCSKFIPILEQSIKLFEENQNFLSSSAVRDTSQLLLLLSDGGLSEDVSDPKFISLIREAHNKGIFVVVILLDHADSKDSILNLKMVIDDGCDFVRFLDVFPLQNYTVLQDVTKLPTLLTDVLRQWFELSSSADKGPGR
Sbjct: 1 MLFNVRDDVNRFINDCNEEVIDYQEIEQDDFSTCSSLFNIITEPKKSLFLRHVKALKKTLKTQQVVNKNEKLIACQLFLQYSTLDDMPNRLEFVEFDVDLATVCKKIADAIPKFLNSTNRYLPIDFRWLPNWKEFQLADFQEINRFLFSIYNIENHLLELKSMEILRNTRPSELTQKAIKKFNVAQLSKLVNTGKLSQNHLSNLVGFDKSIGQFIDQFLSIDISSNFCGMDNFFEMRNVCKLFKRFIEYYPSESFHWRSTCVYHLTSIFNCLTKKQNSDFNDLVKICEQILDKINKIDLSRCLEFVENDEIKDAGIKRNDLRINYPHSPLPVIESQVENQLIIGSEKSKSVWKPSGEEELKKGLCKTFFSLSHIEEPQNMNINELVNHFDVETVRIWPFTLNLLSHILNSCTRPSSIDPIQKFMSGNSEIKNFLPPLPCNFQEKLPEFSDVEKFSTKFTSLADWSNYIIRQGHLIKVISSCSYYFDSDSDEYQLVLDSSTSYLQSQINKIWRNPDCAVFLLVNGRRCHNDTIGYFNSVCTTNSTKRCIRSIETRKSQLEFSKQIYIECASILTSEQIRIARELKLLKELSNIQRHCIEACWNGLGCLEEWLKTRFPWFLSLENDIFNLNPTESSDMMLIIGKLWIRWGSVQMKLFALMESLDPQSQLFWESKYLNEEFDQVSKEKQLGNLYREYLTGVKQNNITERCWSEHGLQHPLLHSMSMRIDQLQSSLAKLTENVGLRPCGDEKYNNLRLKTLRYLSKYGWNALCNESMESLLEFDSTFVAEWSLASSEMLDFFFQQENSSMFPDLIEPLILGISRVLVGIEIINSKTIKQNEKSESASKICEIFCRFPDNNINEMVERILNEETSILRCCQVLIGDCTPSMLAKIHIRLLHLCLRLLNFSQSNKTEKLALLHRLLSVFGNLWAKQQQKQLEKEEAESSMYVEKNKLTENLTEEQIEDLEIFMNVCPSDQQRFDDQHMQMSASMSSAAMKLQEMQQRLTSISSGLTLDEDTIHSVVNLTTLFLNSSHVEEKQDTTVFRDCYRFAAALISADNQGIPSDDCKLFAANFSALNWEYETLSPGVPQNTIGRPHDIYQDPIDVDNLLQAQKILPNANVSVTKLLGIYPEHTTLINIQKKINNILNLSGDYSLIVFINNVERLIHEIQQWEVVAARHVSLIQHVDELNGLLVSWRHQELTMWENTISTANWQLERAVSREFFNHYNLIVQESNKIQYDNDFESINLILEPILNSLLNSTVGNFEYRLKTMKLSIESLISNREIDYRFKDRLKNILLNHYDFYRLYLGNVQEHLSSEYKRIGKELRDFIKTKRWGKTPPKITDVLKCHKIVFKYKRQWSGIINQPCTKFFVLTPINALKQVNLIDIGNCIESDIDFSKSKLSDWCATMLEYSREMWETMENFQKETKELEDISVVQSKSEDDKEALEKKWRMNVKNLQQRKRNCVSQFVKELREMGLLAHGSDLPRNTALMTHLPLGDIENSQLPFSTIRCLRLRLEVDDSDKSEQFAKFNDFKNRLISICDHLLSIACTNTTKIKHFQNEFNIIGNSIEYLSNTCQSEIIAIQDPTDIERNFSLMTSNNSEQINQWDLFIEILKGATCSRRFPSLLTDDLKPSLLTSFDDRDFLKSLTKQICQVNVPKSYLVNMKHMECLSQQKSIIKGINELIEIVKNRFSPENKKINSSITESLLKFNDSNERAIIEIETSLHVANDNFMDTDDSFDGFVNELAVVIRDKISAFKTILLTDNVDKSFIKVNTHINALEQSLDTQKILDLFTKIKSFFCDLNMNQVNKFRNLINEMVLPKLRSLEKVEKEFLGLLLNYHRVLISATDALLTVTTKLMKEGFCIPNDSAWNNEAENSNPSEKMNDFEMAGMDGGVGTNDVTDEIESESQIEGNNKDKEKDETENQPDIPDEDDGIEMTDDFNGKMHDPEIDESKENDNNNEELNDLEDKMGEVGNAPETDVNKEAWDNEKPEEENEKEDKNEDKRKGGVSEQNTTEMVADENEDKDDNSKSEGNNKENSEELKEEESTETDAGNEEDIGEFKDDEDSKDQ----ENTGQEPEKLDNPEEENNPLDDFWNDECQLNVESDSDQVENNGIPDVEEKEENIDDLQPIDETPEDKEEVISGKVEQEFNMAGGYEINENHEMTLDNENVEENTEIGATGNGSGSSNSMNTENAEGNIDNNEPKFDNKESSDKKQDVSSKPSENRSLGDVNQTFKRKAEILDENNADDNKRPRTTDAFSHIDPENPVESTEQILDDATAEQQQHIPEINEEENVTIEENYAIENEKNAELETNNLINEVDQNDNKKSENRERTEIIIQPEEKKIERFTWSQYESKTEYLSRQLCEMLRIVLEPTKANKLGGAYRTGKRLNMKKVIPYIASGFRKDKIWLRRNKPSQREYQVIIGVDNSKSMAYNVNHQLFFESLSIVLNGLTFLEVGQVGLCKFGAKAEVVLPLYEKFSSEIGDRLIETFDFSDKCSKFIPILEQSIKLFEENQNFLSSSAVRDTSQLLLLLSDGGLSEDVSDPKFISLIREAHNKGIFVVVILLDHADSKDSILNLKMVIDDGCDFVRFLDVFPLQNYTVLQDVTKLPTLLTDVLRQWFELSSSADKGPGR 2631
BLAST of Midasin vs. Planmine SMEST
Match: SMESG000033713.1 (SMESG000033713.1) HSP 1 Score: 775.393 bits (2001), Expect = 0.000e+0 Identity = 384/386 (99.48%), Postives = 386/386 (100.00%), Query Frame = 2
Query: 2 EIEKSNSVAGIIVRFLLIVQKMSHEINFSVVTIRDLLSWVNFINISIGNKTSLYQSIFHGAYMVIIDSINDKLNKSFYEELLIKKLDYLVKSSFPDIVECLDFQSSRFELSVKENKVGIDPFYIELGSHSDWXXXXXXXXXXKNFQYSFNSKTTGSNVYRLFRALQVSKKPLLLEGSPGVGKTSLVTQLAKLVGFKVVRINLSEQTDANDLFGCFLPNENGKAGELSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPEINQKFMVEPGKTRLFACQNPVSEGSGRKGLPKSFVNRFTQVYMSSLSDSDYRNILNCLYPAIARETIGFIVEFVQNLREHVVVMKKFGTKGSPWEFNLRDLCRWCDLMVKHQH 1159
EIEKSNSVAGIIVRFLLIVQKMSHEINFSVVTIRDLLSWVNFINISIGNKTSLYQSIFHGAYMVIIDSINDKLNKSFYEELLIKKLDYLVKSSFPDIVECLDFQSSRFELSVKENKVGIDPFYIELGSHSDW+LLSNLDNSSKNFQYSFNSKTTGSNVYRLFRALQVSKKPLLLEGSPGVGKTSLVTQLAKLVGFKVVRINLSEQTDANDLFGCFLPNENGKAGELSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPEINQKF+VEPGKTRLFACQNPVSEGSGRKGLPKSFVNRFTQVYMSSLSDSDYRNILNCLYPAIARETIGFIVEFVQNLREHVVVMKKFGTKGSPWEFNLRDLCRWCDLMVKHQH
Sbjct: 1300 EIEKSNSVAGIIVRFLLIVQKMSHEINFSVVTIRDLLSWVNFINISIGNKTSLYQSIFHGAYMVIIDSINDKLNKSFYEELLIKKLDYLVKSSFPDIVECLDFQSSRFELSVKENKVGIDPFYIELGSHSDWSLLSNLDNSSKNFQYSFNSKTTGSNVYRLFRALQVSKKPLLLEGSPGVGKTSLVTQLAKLVGFKVVRINLSEQTDANDLFGCFLPNENGKAGELSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPEINQKFLVEPGKTRLFACQNPVSEGSGRKGLPKSFVNRFTQVYMSSLSDSDYRNILNCLYPAIARETIGFIVEFVQNLREHVVVMKKFGTKGSPWEFNLRDLCRWCDLMVKHQH 1685 HSP 2 Score: 439.499 bits (1129), Expect = 0.000e+0 Identity = 213/219 (97.26%), Postives = 215/219 (98.17%), Query Frame = 1
Query: 1159 RTKEDQDACGKVYNELADKSRFARFIPSGLDFYITDQVLKIGQTQLSVNIANNTDNSKQFNKHLLLAGQLAILESMMLTVDMGWLTVLVGPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGSFEQQKEGGKFKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGLVYGDRIEEIKPHKNFRLFLILDPDCGGEISK 1815
RTKEDQDACGKVYNELADKSRFARFIPSGLDFYITDQVLKIGQTQLSVNIANNTDNSKQFNKHLLLAGQLAILESMMLTVDMGWLTVLVGPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGSFEQQKEGGKFKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERG VYGDR+EEIKPHKNFRLFLILDPDCG + K
Sbjct: 1703 RTKEDQDACGKVYNELADKSRFARFIPSGLDFYITDQVLKIGQTQLSVNIANNTDNSKQFNKHLLLAGQLAILESMMLTVDMGWLTVLVGPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGSFEQQKEGGKFKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGSVYGDRLEEIKPHKNFRLFLILDPDCGEKFLK 1921 HSP 3 Score: 119.783 bits (299), Expect = 3.531e-26 Identity = 80/240 (33.33%), Postives = 118/240 (49.17%), Query Frame = 2
Query: 452 SKTTGSNVYRLFRALQVSK-KPLLLEGSPGVGKTSLVTQLAKLVGFKVVRINLSEQTDANDLFGCFLP-NENGKAGELSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPEINQKFMVEPGKTRLFACQNPVSEGSGRKGLPKSFVNRFTQVYMSSLSDSDYRNILN--CLYPAIARETIGFIVEFVQNLREHVVVMKKFGTKGSPWE--------FNLRDLCRWC 1135
+++ N+ L R + K P+L++G VGKTSL+T LAKL G R+N E TD + G ++ N + L +++G L+QA++ G WI+LDE+NLA +LE LN LD EIFI E P RLFA QNP GRK L ++ NRF +++ + + + + IL+ C P R+ V M+K + + +LRDL RW
Sbjct: 780 TESVSKNLTDLARIVSAGKILPVLVQGETSVGKTSLITYLAKLTGHICHRVNNHETTDIQEYIGSYVQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVETQTTIKAHP-MFRLFATQNPPGLYGGRKVLSRALRNRFIELHFNPIPNCELVEILDESCKIPK-------------NKGRKMVEAMRKLQIESQELDVVHCKRNYISLRDLFRWA 1005 HSP 4 Score: 78.9518 bits (193), Expect = 8.399e-14 Identity = 53/161 (32.92%), Postives = 87/161 (54.04%), Query Frame = 2
Query: 680 SWRDGPLLQALKAGDWILLDEMNLASQSVLE---------CLNACLDHRG-EIFIPEINQKFMVEPGKTRLFACQNPVSEGSGRKGLPKSFVNRFTQVYMSSLSDSDYRNILNCLY-PAIARETIGFIVEFVQNLREHVVVMKKFGTKGSPWEFNLRDLCR 1129
++ +G L++A++ GDW+LLDE+NLA +L+ + L +G E +P +Q F RLFAC NP ++ G++ LP+ NRFT+VY+ L+D + N++ Y +++ + IV F LR+ V G+ +F+LR LCR
Sbjct: 521 AYIEGALVKAIRNGDWVLLDEINLAPPEMLQGLSGLLESSSGSVTLTEKGDETPVPR-HQDF-------RLFACMNPATD-VGKQNLPQGLRNRFTEVYLHELTDINELNVIVKYYLGSLSGRIVESIVNFYLTLRKGVKENSIIDGGGNKPQFSLRTLCR 672 HSP 5 Score: 77.7962 bits (190), Expect = 1.865e-13 Identity = 53/159 (33.33%), Postives = 84/159 (52.83%), Query Frame = 1
Query: 1414 TVLVGPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGSFE-----QQKEGGKFKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERG----LVYGDRIEEIK--PHKNFRLFLILDPDCGG-----EISKAMRNRGLEI 1842
+LVG G GKTTI +L + + L +++ + + +D LG + + E F+WVD LV A++QG ++D ++ SVL+RLN +LE + L L E G L I+ ++ KNF++ ++P GG E+S A+RNR EI
Sbjct: 1114 VLLVGNTGCGKTTICQLAAMLNQQELLTINCHMHTEASDFLGGLKPAVNKTEDETRLFQWVDGPLVVAMKQGKMFLIDEISLADDSVLERLNSVLEKDRKLTLVENGDANLLTSETEIQSVQICADKNFQVVATMNP--GGDFGKKELSPALRNRFTEI 1270 HSP 6 Score: 77.0258 bits (188), Expect = 3.387e-13 Identity = 56/175 (32.00%), Postives = 87/175 (49.71%), Query Frame = 2
Query: 482 LFRALQVSKKPLLLEGSPGVGKTSLVTQLAKLVGFKVVRINLSEQTDANDLFGCFLPNENGKAGE---LSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFI------------PEINQKFMVEPGKTRLFACQNPVSEGSGRKGLPKSFVNRFTQVYMSSLS 961
L R++Q + P+LL G+ G GKT++ A L +++ IN T+A+D G P N E W DGPL+ A+K G L+DE++LA SVLE LN+ L+ ++ + EI + ++ A NP + G+K L + NRFT+++ S S
Sbjct: 1104 LIRSIQCDE-PVLLVGNTGCGKTTICQLAAMLNQQELLTINCHMHTEASDFLGGLKPAVNKTEDETRLFQWVDGPLVVAMKQGKMFLIDEISLADDSVLERLNSVLEKDRKLTLVENGDANLLTSETEIQSVQICADKNFQVVATMNPGGD-FGKKELSPALRNRFTEIWCPSDS 1276 HSP 7 Score: 76.6406 bits (187), Expect = 3.842e-13 Identity = 53/172 (30.81%), Postives = 87/172 (50.58%), Query Frame = 1
Query: 1417 VLVGPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGSFEQQKEG--GKFKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGLVYGDRIEEIKPHKNFRLFLILDPDCGGE----ISKAMRNRGLEICIIPENRELTLTDNDLLAILITIY 1914
+L G G GKT++V L+ + G + ++++ D DL G F + G G+ W D L+QAL+ GDW++LD +N S SVL+ LN L+ G + + E + ++P K RLF +P G + K+ NR ++ + +L+D+D IL +Y
Sbjct: 1472 LLEGSPGVGKTSLVTQLAKLVGFKVVRINLSEQTDANDLFGCFLPNENGKAGELSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPEINQKF-----LVEPGKT-RLFACQNPVSEGSGRKGLPKSFVNRFTQVYMS------SLSDSDYRNILNCLY 1631 HSP 8 Score: 75.485 bits (184), Expect = 8.751e-13 Identity = 50/156 (32.05%), Postives = 79/156 (50.64%), Query Frame = 1
Query: 1411 LTVLV-GPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGSFEQQKEG---GKFKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGLVYGDRIEEIKPHKNFRLFLILDPD--CGGE--ISKAMRNRGLEICIIP 1854
L VLV G GKT+++ L+ +TG+ ++ + D + +GS+ QQ + + LVQA+R G W+ILD +N +L+ LN +L+ N + + E IK H FRLF +P GG +S+A+RNR +E+ P
Sbjct: 800 LPVLVQGETSVGKTSLITYLAKLTGHICHRVNNHETTDIQEYIGSYVQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVETQTT-------IKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIELHFNP 948 HSP 9 Score: 75.0998 bits (183), Expect = 1.117e-12 Identity = 43/127 (33.86%), Postives = 68/127 (53.54%), Query Frame = 2
Query: 518 LLEGSPGVGKTSLVTQLAKLVGFKVVRINLSEQTDANDLFGCFLPNENGKAGELSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPEINQKF-----MVEPGKT-RLFACQNP 880
+L G G GKT++V L+ + G + ++++ D DL G F + G G+ W D L+QAL+ GDW++LD +N S SVL+ LN L+ G + + E + ++P K RLF +P
Sbjct: 1789 VLVGPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGSFEQQKEG--GKFKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGSVYGDRLEEIKPHKNFRLFLILDP 1913 HSP 10 Score: 74.3294 bits (181), Expect = 1.772e-12 Identity = 40/130 (30.77%), Postives = 71/130 (54.62%), Query Frame = 2
Query: 437 QYSFNSKTTGSNVYRLFRALQVSKKPLLLEGSPGVGKTSLVTQLAKLVGFKVVRINLSEQTDANDLFGCFLPNENGKAGELSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPEIN 826
+ +F S + +LFR + P+LL G G GK+ L+ +AKL+G ++ R+ +S+++D+ L G + + GE WR GPL+ A+K G WI+L+++ ++L L L+ P+I+
Sbjct: 9 EINFVSTNQTVKIIKLFRFCIEKQMPILLTGPVGSGKSVLIDYMAKLMGKELCRVQISDESDSKTLLGGYCCTD--IPGEFEWRSGPLITAMKRGQWIILEDILSGQSNLLGMLRPLLEAESLGLKPKIS 136 HSP 11 Score: 63.1586 bits (152), Expect = 4.678e-9 Identity = 36/121 (29.75%), Postives = 65/121 (53.72%), Query Frame = 1
Query: 1417 VLVGPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGSFEQQKEGGKFKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGLVYGDRIEEIKPHKNFRLFL 1779
+L GP G+GK+ ++ ++ + G L + ++ D+ LLG + G+F+W L+ A+++G W+IL+ + +++L L LLE SL L + EEI H++FR+ L
Sbjct: 36 LLTGPVGSGKSVLIDYMAKLMGKELCRVQISDESDSKTLLGGYCCTDIPGEFEWRSGPLITAMKRGQWIILEDILSGQSNLLGMLRPLLEAE-SLGLKPKISNITSLNEEIIAHEDFRIIL 155
BLAST of Midasin vs. Planmine SMEST
Match: SMESG000033713.1 (SMESG000033713.1) HSP 1 Score: 774.622 bits (1999), Expect = 0.000e+0 Identity = 384/386 (99.48%), Postives = 386/386 (100.00%), Query Frame = 2
Query: 2 EIEKSNSVAGIIVRFLLIVQKMSHEINFSVVTIRDLLSWVNFINISIGNKTSLYQSIFHGAYMVIIDSINDKLNKSFYEELLIKKLDYLVKSSFPDIVECLDFQSSRFELSVKENKVGIDPFYIELGSHSDWXXXXXXXXXXKNFQYSFNSKTTGSNVYRLFRALQVSKKPLLLEGSPGVGKTSLVTQLAKLVGFKVVRINLSEQTDANDLFGCFLPNENGKAGELSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPEINQKFMVEPGKTRLFACQNPVSEGSGRKGLPKSFVNRFTQVYMSSLSDSDYRNILNCLYPAIARETIGFIVEFVQNLREHVVVMKKFGTKGSPWEFNLRDLCRWCDLMVKHQH 1159
EIEKSNSVAGIIVRFLLIVQKMSHEINFSVVTIRDLLSWVNFINISIGNKTSLYQSIFHGAYMVIIDSINDKLNKSFYEELLIKKLDYLVKSSFPDIVECLDFQSSRFELSVKENKVGIDPFYIELGSHSDW+LLSNLDNSSKNFQYSFNSKTTGSNVYRLFRALQVSKKPLLLEGSPGVGKTSLVTQLAKLVGFKVVRINLSEQTDANDLFGCFLPNENGKAGELSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPEINQKF+VEPGKTRLFACQNPVSEGSGRKGLPKSFVNRFTQVYMSSLSDSDYRNILNCLYPAIARETIGFIVEFVQNLREHVVVMKKFGTKGSPWEFNLRDLCRWCDLMVKHQH
Sbjct: 1322 EIEKSNSVAGIIVRFLLIVQKMSHEINFSVVTIRDLLSWVNFINISIGNKTSLYQSIFHGAYMVIIDSINDKLNKSFYEELLIKKLDYLVKSSFPDIVECLDFQSSRFELSVKENKVGIDPFYIELGSHSDWSLLSNLDNSSKNFQYSFNSKTTGSNVYRLFRALQVSKKPLLLEGSPGVGKTSLVTQLAKLVGFKVVRINLSEQTDANDLFGCFLPNENGKAGELSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPEINQKFLVEPGKTRLFACQNPVSEGSGRKGLPKSFVNRFTQVYMSSLSDSDYRNILNCLYPAIARETIGFIVEFVQNLREHVVVMKKFGTKGSPWEFNLRDLCRWCDLMVKHQH 1707 HSP 2 Score: 439.113 bits (1128), Expect = 0.000e+0 Identity = 213/219 (97.26%), Postives = 215/219 (98.17%), Query Frame = 1
Query: 1159 RTKEDQDACGKVYNELADKSRFARFIPSGLDFYITDQVLKIGQTQLSVNIANNTDNSKQFNKHLLLAGQLAILESMMLTVDMGWLTVLVGPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGSFEQQKEGGKFKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGLVYGDRIEEIKPHKNFRLFLILDPDCGGEISK 1815
RTKEDQDACGKVYNELADKSRFARFIPSGLDFYITDQVLKIGQTQLSVNIANNTDNSKQFNKHLLLAGQLAILESMMLTVDMGWLTVLVGPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGSFEQQKEGGKFKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERG VYGDR+EEIKPHKNFRLFLILDPDCG + K
Sbjct: 1725 RTKEDQDACGKVYNELADKSRFARFIPSGLDFYITDQVLKIGQTQLSVNIANNTDNSKQFNKHLLLAGQLAILESMMLTVDMGWLTVLVGPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGSFEQQKEGGKFKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGSVYGDRLEEIKPHKNFRLFLILDPDCGEKFLK 1943 HSP 3 Score: 119.783 bits (299), Expect = 2.898e-26 Identity = 80/240 (33.33%), Postives = 118/240 (49.17%), Query Frame = 2
Query: 452 SKTTGSNVYRLFRALQVSK-KPLLLEGSPGVGKTSLVTQLAKLVGFKVVRINLSEQTDANDLFGCFLP-NENGKAGELSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPEINQKFMVEPGKTRLFACQNPVSEGSGRKGLPKSFVNRFTQVYMSSLSDSDYRNILN--CLYPAIARETIGFIVEFVQNLREHVVVMKKFGTKGSPWE--------FNLRDLCRWC 1135
+++ N+ L R + K P+L++G VGKTSL+T LAKL G R+N E TD + G ++ N + L +++G L+QA++ G WI+LDE+NLA +LE LN LD EIFI E P RLFA QNP GRK L ++ NRF +++ + + + + IL+ C P R+ V M+K + + +LRDL RW
Sbjct: 802 TESVSKNLTDLARIVSAGKILPVLVQGETSVGKTSLITYLAKLTGHICHRVNNHETTDIQEYIGSYVQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVETQTTIKAHP-MFRLFATQNPPGLYGGRKVLSRALRNRFIELHFNPIPNCELVEILDESCKIPK-------------NKGRKMVEAMRKLQIESQELDVVHCKRNYISLRDLFRWA 1027 HSP 4 Score: 78.9518 bits (193), Expect = 7.177e-14 Identity = 53/161 (32.92%), Postives = 87/161 (54.04%), Query Frame = 2
Query: 680 SWRDGPLLQALKAGDWILLDEMNLASQSVLE---------CLNACLDHRG-EIFIPEINQKFMVEPGKTRLFACQNPVSEGSGRKGLPKSFVNRFTQVYMSSLSDSDYRNILNCLY-PAIARETIGFIVEFVQNLREHVVVMKKFGTKGSPWEFNLRDLCR 1129
++ +G L++A++ GDW+LLDE+NLA +L+ + L +G E +P +Q F RLFAC NP ++ G++ LP+ NRFT+VY+ L+D + N++ Y +++ + IV F LR+ V G+ +F+LR LCR
Sbjct: 543 AYIEGALVKAIRNGDWVLLDEINLAPPEMLQGLSGLLESSSGSVTLTEKGDETPVPR-HQDF-------RLFACMNPATD-VGKQNLPQGLRNRFTEVYLHELTDINELNVIVKYYLGSLSGRIVESIVNFYLTLRKGVKENSIIDGGGNKPQFSLRTLCR 694 HSP 5 Score: 77.7962 bits (190), Expect = 1.719e-13 Identity = 53/159 (33.33%), Postives = 84/159 (52.83%), Query Frame = 1
Query: 1414 TVLVGPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGSFE-----QQKEGGKFKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERG----LVYGDRIEEIK--PHKNFRLFLILDPDCGG-----EISKAMRNRGLEI 1842
+LVG G GKTTI +L + + L +++ + + +D LG + + E F+WVD LV A++QG ++D ++ SVL+RLN +LE + L L E G L I+ ++ KNF++ ++P GG E+S A+RNR EI
Sbjct: 1136 VLLVGNTGCGKTTICQLAAMLNQQELLTINCHMHTEASDFLGGLKPAVNKTEDETRLFQWVDGPLVVAMKQGKMFLIDEISLADDSVLERLNSVLEKDRKLTLVENGDANLLTSETEIQSVQICADKNFQVVATMNP--GGDFGKKELSPALRNRFTEI 1292 HSP 6 Score: 77.0258 bits (188), Expect = 2.919e-13 Identity = 56/175 (32.00%), Postives = 87/175 (49.71%), Query Frame = 2
Query: 482 LFRALQVSKKPLLLEGSPGVGKTSLVTQLAKLVGFKVVRINLSEQTDANDLFGCFLPNENGKAGE---LSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFI------------PEINQKFMVEPGKTRLFACQNPVSEGSGRKGLPKSFVNRFTQVYMSSLS 961
L R++Q + P+LL G+ G GKT++ A L +++ IN T+A+D G P N E W DGPL+ A+K G L+DE++LA SVLE LN+ L+ ++ + EI + ++ A NP + G+K L + NRFT+++ S S
Sbjct: 1126 LIRSIQCDE-PVLLVGNTGCGKTTICQLAAMLNQQELLTINCHMHTEASDFLGGLKPAVNKTEDETRLFQWVDGPLVVAMKQGKMFLIDEISLADDSVLERLNSVLEKDRKLTLVENGDANLLTSETEIQSVQICADKNFQVVATMNPGGD-FGKKELSPALRNRFTEIWCPSDS 1298 HSP 7 Score: 76.6406 bits (187), Expect = 3.662e-13 Identity = 53/172 (30.81%), Postives = 87/172 (50.58%), Query Frame = 1
Query: 1417 VLVGPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGSFEQQKEG--GKFKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGLVYGDRIEEIKPHKNFRLFLILDPDCGGE----ISKAMRNRGLEICIIPENRELTLTDNDLLAILITIY 1914
+L G G GKT++V L+ + G + ++++ D DL G F + G G+ W D L+QAL+ GDW++LD +N S SVL+ LN L+ G + + E + ++P K RLF +P G + K+ NR ++ + +L+D+D IL +Y
Sbjct: 1494 LLEGSPGVGKTSLVTQLAKLVGFKVVRINLSEQTDANDLFGCFLPNENGKAGELSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPEINQKF-----LVEPGKT-RLFACQNPVSEGSGRKGLPKSFVNRFTQVYMS------SLSDSDYRNILNCLY 1653 HSP 8 Score: 75.485 bits (184), Expect = 8.064e-13 Identity = 50/156 (32.05%), Postives = 79/156 (50.64%), Query Frame = 1
Query: 1411 LTVLV-GPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGSFEQQKEG---GKFKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGLVYGDRIEEIKPHKNFRLFLILDPD--CGGE--ISKAMRNRGLEICIIP 1854
L VLV G GKT+++ L+ +TG+ ++ + D + +GS+ QQ + + LVQA+R G W+ILD +N +L+ LN +L+ N + + E IK H FRLF +P GG +S+A+RNR +E+ P
Sbjct: 822 LPVLVQGETSVGKTSLITYLAKLTGHICHRVNNHETTDIQEYIGSYVQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVETQTT-------IKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIELHFNP 970 HSP 9 Score: 75.485 bits (184), Expect = 9.949e-13 Identity = 43/127 (33.86%), Postives = 68/127 (53.54%), Query Frame = 2
Query: 518 LLEGSPGVGKTSLVTQLAKLVGFKVVRINLSEQTDANDLFGCFLPNENGKAGELSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPEINQKF-----MVEPGKT-RLFACQNP 880
+L G G GKT++V L+ + G + ++++ D DL G F + G G+ W D L+QAL+ GDW++LD +N S SVL+ LN L+ G + + E + ++P K RLF +P
Sbjct: 1811 VLVGPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGSFEQQKEG--GKFKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGSVYGDRLEEIKPHKNFRLFLILDP 1935 HSP 10 Score: 74.7146 bits (182), Expect = 1.540e-12 Identity = 40/130 (30.77%), Postives = 71/130 (54.62%), Query Frame = 2
Query: 437 QYSFNSKTTGSNVYRLFRALQVSKKPLLLEGSPGVGKTSLVTQLAKLVGFKVVRINLSEQTDANDLFGCFLPNENGKAGELSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPEIN 826
+ +F S + +LFR + P+LL G G GK+ L+ +AKL+G ++ R+ +S+++D+ L G + + GE WR GPL+ A+K G WI+L+++ ++L L L+ P+I+
Sbjct: 31 EINFVSTNQTVKIIKLFRFCIEKQMPILLTGPVGSGKSVLIDYMAKLMGKELCRVQISDESDSKTLLGGYCCTD--IPGEFEWRSGPLITAMKRGQWIILEDILSGQSNLLGMLRPLLEAESLGLKPKIS 158 HSP 11 Score: 67.0106 bits (162), Expect = 3.298e-10 Identity = 43/180 (23.89%), Postives = 93/180 (51.67%), Query Frame = 1
Query: 1246 LDFYITDQVLKIGQTQLSVNIANNTDNSKQFNKHLLLAGQ--LAILESMMLTVDMGWLTVLVGPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGSFEQQKEGGKFKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGLVYGDRIEEIKPHKNFRLFL 1779
+ ++ Q +K+ + + I+N+ +++ +FN+ ++ + I++ ++ +L GP G+GK+ ++ ++ + G L + ++ D+ LLG + G+F+W L+ A+++G W+IL+ + +++L L LLE SL L + EEI H++FR+ L
Sbjct: 1 MKYFFLFQTIKVDDKK--IKISNDMNSNTKFNEINFVSTNQTVKIIKLFRFCIEKQMPILLTGPVGSGKSVLIDYMAKLMGKELCRVQISDESDSKTLLGGYCCTDIPGEFEWRSGPLITAMKRGQWIILEDILSGQSNLLGMLRPLLEAE-SLGLKPKISNITSLNEEIIAHEDFRIIL 177
BLAST of Midasin vs. Planmine SMEST
Match: SMESG000062774.1 (SMESG000062774.1) HSP 1 Score: 55.0694 bits (131), Expect = 1.268e-6 Identity = 62/243 (25.51%), Postives = 104/243 (42.80%), Query Frame = 1
Query: 1369 AILESMMLTVDMGWLTVLVGPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGSFEQQKEGGKFKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGLVYGDRIEEIKPHK-----------------NFRLFLILDPD-CGGEISKAMRNRGLEICIIPEN---------RELTLTDNDLLAILITIYKLSVKNADSLLRF-HKTMILAQSETPQIKIGNIN 2013
IL+ ++++ +G V +GP G GK+ IV+ L++ G+ + + + M D+L E G W +S +V+ +G W +L+ ++ + L+ L+ L L+ DR + I+ + NFR+ + DP GE SK N E + PE EL+ D L+ + TI +S D LL HK L S+ PQI+ ++N
Sbjct: 327 GILQQLIVSHSLGDFCV-IGPQGCGKSIIVEALANEFGSVVTHVVLYGDMTVRDILQQRETDSATGDTIWRNSAIVRTAIEGGWAVLEGLHRLEKGCMSVLSQLVTDRQLQLFDGTRLIRHDRYDAIRHEEGLNNEQMASRGIYRIDPNFRIIALADPPLIHGESSKTGSNNKTE-WLTPEILTSFRFHMLPELSREDEKLI-VTKTIPNVSASKLDKLLDIVHK---LRTSDDPQIRDLSLN 563
BLAST of Midasin vs. Planmine SMEST
Match: SMESG000062774.1 (SMESG000062774.1) HSP 1 Score: 55.0694 bits (131), Expect = 1.333e-6 Identity = 62/243 (25.51%), Postives = 104/243 (42.80%), Query Frame = 1
Query: 1369 AILESMMLTVDMGWLTVLVGPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGSFEQQKEGGKFKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGLVYGDRIEEIKPHK-----------------NFRLFLILDPD-CGGEISKAMRNRGLEICIIPEN---------RELTLTDNDLLAILITIYKLSVKNADSLLRF-HKTMILAQSETPQIKIGNIN 2013
IL+ ++++ +G V +GP G GK+ IV+ L++ G+ + + + M D+L E G W +S +V+ +G W +L+ ++ + L+ L+ L L+ DR + I+ + NFR+ + DP GE SK N E + PE EL+ D L+ + TI +S D LL HK L S+ PQI+ ++N
Sbjct: 327 GILQQLIVSHSLGDFCV-IGPQGCGKSIIVEALANEFGSVVTHVVLYGDMTVRDILQQRETDSATGDTIWRNSAIVRTAIEGGWAVLEGLHRLEKGCMSVLSQLVTDRQLQLFDGTRLIRHDRYDAIRHEEGLNNEQMASRGIYRIDPNFRIIALADPPLIHGESSKTGSNNKTE-WLTPEILTSFRFHMLPELSREDEKLI-VTKTIPNVSASKLDKLLDIVHK---LRTSDDPQIRDLSLN 563 The following BLAST results are available for this feature:
BLAST of Midasin vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of Midasin vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 5
BLAST of Midasin vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 1
BLAST of Midasin vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 1
BLAST of Midasin vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 4
BLAST of Midasin vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 2
BLAST of Midasin vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of Midasin vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of Midasin vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 1
BLAST of Midasin vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 2
BLAST of Midasin vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 1
BLAST of Midasin vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of Midasin vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 1
BLAST of Midasin vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30023713 ID=SMED30023713|Name=Midasin|organism=Schmidtea mediterranea sexual|type=transcript|length=10036bpback to top protein sequence of SMED30023713-orf-1 >SMED30023713-orf-1 ID=SMED30023713-orf-1|Name=SMED30023713-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=2872bp MMLTVDMGWLTVLVGPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLback to top protein sequence of SMED30023713-orf-2 >SMED30023713-orf-2 ID=SMED30023713-orf-2|Name=SMED30023713-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=402bp EIEKSNSVAGIIVRFLLIVQKMSHEINFSVVTIRDLLSWVNFINISIGNKback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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