General transcription factor II-I repeat domain-containing protein 2
Overview
Neoblast Clusters
Zeng et. al., 2018
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny AppEmbryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30023683 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 5
Homology
BLAST of General transcription factor II-I repeat domain-containing protein 2 vs. Ensembl Zebrafish
Match: CR392001.3 (pep chromosome:GRCz11:8:38963323:38965260:-1 gene:ENSDARG00000117159.1 transcript:ENSDART00000181495.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:CR392001.3) HSP 1 Score: 55.8398 bits (133), Expect = 7.363e-10 Identity = 25/45 (55.56%), Postives = 31/45 (68.89%), Query Frame = 1 Query: 1 TTASFAAALAIGRNGKPFSDCEYLTTALIQCSEHLFNDFKNKREI 135 T ASF A I + GKPF+D +Y+ + I SEHLF+DFKNK EI Sbjct: 102 TAASFVATREIIKRGKPFTDGDYMKESFINISEHLFSDFKNKTEI 146 HSP 2 Score: 45.4394 bits (106), Expect = 3.779e-6 Identity = 28/88 (31.82%), Postives = 37/88 (42.05%), Query Frame = 1 Query: 148 LELVDLRSSTLWTDKFKTLRGDLEKTEENHLDM------------------ISKCWASLPTKLTCIKKVAFVLLSGFGSSYYCEQRFS 357 +E+ D+ LW KFK L +LE + I W +LP +K AF +LS FGS+Y CEQ FS Sbjct: 506 MEMADISDKDLWVSKFKRLTAELEDVTRQKAQLAQSHKWSEMEGLPVPEKLIYDTWNALPDCYKNMKTYAFGVLSIFGSTYLCEQIFS 593
BLAST of General transcription factor II-I repeat domain-containing protein 2 vs. TrEMBL
Match: A0A1A8V8N3 (Uncharacterized protein OS=Nothobranchius furzeri OX=105023 GN=Nfu_g_1_015633 PE=4 SV=1) HSP 1 Score: 72.0182 bits (175), Expect = 8.468e-14 Identity = 35/72 (48.61%), Postives = 46/72 (63.89%), Query Frame = 1 Query: 148 LELVDLRSSTLWTDKFKTLRGDLEKTE-ENHLDMISKCWASLPTKLTCIKKVAFVLLSGFGSSYYCEQRFSQ 360 ++L++L+SSTLW KF LR LE T +H I CW SLP + C+K +A LL+ FGS+Y CEQ FS Sbjct: 3 MQLIELKSSTLWGTKFAELRQQLESTAVHDHGACIFSCWTSLPDRFNCLKNIAQALLTVFGSTYLCEQIFSH 74
BLAST of General transcription factor II-I repeat domain-containing protein 2 vs. TrEMBL
Match: A0A087TCS8 (General transcription factor II-I repeat domain-containing protein 2A (Fragment) OS=Stegodyphus mimosarum OX=407821 GN=X975_02296 PE=4 SV=1) HSP 1 Score: 75.0998 bits (183), Expect = 2.812e-13 Identity = 33/49 (67.35%), Postives = 39/49 (79.59%), Query Frame = 1 Query: 133 ITNCCLELVDLRSSTLWTDKFKTLRGDLEKTEENHLDMISKCWASLPTK 279 I + LE++D +SSTLWTDKFK LR DLE TE+NH MIS CWASLPT+ Sbjct: 349 IDSFSLEMIDFKSSTLWTDKFKKLRNDLENTEDNHFLMISTCWASLPTR 397
BLAST of General transcription factor II-I repeat domain-containing protein 2 vs. TrEMBL
Match: A0A1A8EEQ0 (Uncharacterized protein OS=Nothobranchius kadleci OX=1051664 GN=Nfu_g_1_015633 PE=4 SV=1) HSP 1 Score: 68.9366 bits (167), Expect = 1.690e-12 Identity = 34/72 (47.22%), Postives = 44/72 (61.11%), Query Frame = 1 Query: 148 LELVDLRSSTLWTDKFKTLRGDLEKTE-ENHLDMISKCWASLPTKLTCIKKVAFVLLSGFGSSYYCEQRFSQ 360 ++L+ L+SSTLW KF LR LE T +H I CW S P + C+K +A LL+ FGS+Y CEQ FS Sbjct: 3 MQLIKLKSSTLWGTKFAELRQQLESTAVHDHGACIFSCWTSPPDRFNCLKNIAQALLTVFGSTYLCEQIFSH 74
BLAST of General transcription factor II-I repeat domain-containing protein 2 vs. TrEMBL
Match: A0A087T138 (Zinc finger MYM-type protein 6 (Fragment) OS=Stegodyphus mimosarum OX=407821 GN=X975_18236 PE=4 SV=1) HSP 1 Score: 70.4774 bits (171), Expect = 1.200e-11 Identity = 30/45 (66.67%), Postives = 37/45 (82.22%), Query Frame = 1 Query: 1 TTASFAAALAIGRNGKPFSDCEYLTTALIQCSEHLFNDFKNKREI 135 T+A+F AAL+IG NGKPF+D E+L A I CSEHLFNDF NK++I Sbjct: 56 TSANFVAALSIGTNGKPFTDGEFLQNAFINCSEHLFNDFANKKQI 100
BLAST of General transcription factor II-I repeat domain-containing protein 2 vs. TrEMBL
Match: A0A1A8K9W9 (Uncharacterized protein OS=Nothobranchius kuhntae OX=321403 GN=Nfu_g_1_015633 PE=4 SV=1) HSP 1 Score: 68.9366 bits (167), Expect = 2.352e-11 Identity = 35/72 (48.61%), Postives = 45/72 (62.50%), Query Frame = 1 Query: 148 LELVDLRSSTLWTDKFKTLRGDLEKTE-ENHLDMISKCWASLPTKLTCIKKVAFVLLSGFGSSYYCEQRFSQ 360 ++L +L+SSTLW KF LR LE T +H I CW SLP + C+K +A LL+ FGS+Y CEQ FS Sbjct: 207 MQLTELKSSTLWGTKFAELRQLLESTAVHDHGACIYSCWTSLPDRFNCLKNIAQALLTVFGSTYLCEQIFSH 278
BLAST of General transcription factor II-I repeat domain-containing protein 2 vs. Ensembl Cavefish
Match: ENSAMXT00000049756.1 (pep primary_assembly:Astyanax_mexicanus-2.0:21:10276151:10280466:-1 gene:ENSAMXG00000035254.1 transcript:ENSAMXT00000049756.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 47.3654 bits (111), Expect = 5.204e-7 Identity = 21/45 (46.67%), Postives = 29/45 (64.44%), Query Frame = 1 Query: 1 TTASFAAALAIGRNGKPFSDCEYLTTALIQCSEHLFNDFKNKREI 135 T AS+ A I ++GKPF+D EY+ A + C+E LF+D NK I Sbjct: 94 TEASYNLASCIAKHGKPFTDGEYVKEAFLSCAETLFDDLPNKDTI 138
BLAST of General transcription factor II-I repeat domain-containing protein 2 vs. Ensembl Cavefish
Match: ENSAMXT00000053332.1 (pep primary_assembly:Astyanax_mexicanus-2.0:1:2353647:2355530:-1 gene:ENSAMXG00000042872.1 transcript:ENSAMXT00000053332.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 46.9802 bits (110), Expect = 8.084e-7 Identity = 21/45 (46.67%), Postives = 29/45 (64.44%), Query Frame = 1 Query: 1 TTASFAAALAIGRNGKPFSDCEYLTTALIQCSEHLFNDFKNKREI 135 T AS+ A I ++GKPF+D EY+ A + C+E LF+D NK I Sbjct: 94 TEASYNLASCIAKHGKPFTDGEYVKEAFLSCAETLFDDLPNKDTI 138
BLAST of General transcription factor II-I repeat domain-containing protein 2 vs. Ensembl Medaka
Match: ENSORLT00000035623.1 (pep primary_assembly:ASM223467v1:9:5067000:5069708:1 gene:ENSORLG00000026230.1 transcript:ENSORLT00000035623.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 58.5362 bits (140), Expect = 6.833e-11 Identity = 30/89 (33.71%), Postives = 46/89 (51.69%), Query Frame = 1 Query: 148 LELVDLRSSTLWTDKFKTLRGDLEKTEENHLDMIS------------------KCWASLPTKLTCIKKVAFVLLSGFGSSYYCEQRFSQ 360 L+ DL++S LW +KFK+L DLE+ E ++ S K W +LP +++V+ +L+ FGS+Y CEQ FS Sbjct: 539 LQAADLKASDLWVNKFKSLNEDLERLERQKAELASQHKWEEIKKLQPADQLILKTWNALPVTFFTLQRVSVAVLTMFGSTYACEQSFSH 627 HSP 2 Score: 46.595 bits (109), Expect = 1.216e-6 Identity = 20/45 (44.44%), Postives = 32/45 (71.11%), Query Frame = 1 Query: 1 TTASFAAALAIGRNGKPFSDCEYLTTALIQCSEHLFNDFKNKREI 135 +ASFAAALAI +NGKPF+D E+ + ++ + L+ +F +K +I Sbjct: 123 NSASFAAALAIVKNGKPFTDGEFAKSFMLDVANELYANFSDKDKI 167
BLAST of General transcription factor II-I repeat domain-containing protein 2 vs. Ensembl Medaka
Match: ENSORLT00000028910.1 (pep primary_assembly:ASM223467v1:18:2548265:2550717:1 gene:ENSORLG00000029755.1 transcript:ENSORLT00000028910.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 58.5362 bits (140), Expect = 6.870e-11 Identity = 30/89 (33.71%), Postives = 46/89 (51.69%), Query Frame = 1 Query: 148 LELVDLRSSTLWTDKFKTLRGDLEKTEENHLDMIS------------------KCWASLPTKLTCIKKVAFVLLSGFGSSYYCEQRFSQ 360 L+ DL++S LW +KFK+L DLE+ E ++ S K W +LP +++V+ +L+ FGS+Y CEQ FS Sbjct: 512 LQAADLKASDLWVNKFKSLNEDLERLERQKAELASQHKWEEIKKLQPADQLILKTWNALPVTFFTLQRVSVAVLTMFGSTYACEQSFSH 600 HSP 2 Score: 45.0542 bits (105), Expect = 3.510e-6 Identity = 19/42 (45.24%), Postives = 30/42 (71.43%), Query Frame = 1 Query: 1 TTASFAAALAIGRNGKPFSDCEYLTTALIQCSEHLFNDFKNK 126 +ASFAAALAI +NGKPF+D E+ + ++ + L+ +F +K Sbjct: 96 NSASFAAALAIVKNGKPFTDGEFAKSFMLDVANELYANFSDK 137
BLAST of General transcription factor II-I repeat domain-containing protein 2 vs. Ensembl Medaka
Match: ENSORLT00000038486.1 (pep primary_assembly:ASM223467v1:11:14048492:14066451:1 gene:ENSORLG00000022236.1 transcript:ENSORLT00000038486.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 58.5362 bits (140), Expect = 7.615e-11 Identity = 29/87 (33.33%), Postives = 46/87 (52.87%), Query Frame = 1 Query: 148 LELVDLRSSTLWTDKFKTLRGDLEKTEENHLDMIS----------------KCWASLPTKLTCIKKVAFVLLSGFGSSYYCEQRFSQ 360 L+ DL++S LW +KFK++ DLE+ E ++ S K W +LP +++V+ +L+ FGS+Y CEQ FS Sbjct: 402 LQAADLKASDLWVNKFKSMNEDLERLERQKAELASQHKWCYEEWLKDQLILKTWNALPVTFFTLQRVSVAVLTMFGSTYACEQSFSH 488 HSP 2 Score: 45.8246 bits (107), Expect = 2.095e-6 Identity = 20/45 (44.44%), Postives = 33/45 (73.33%), Query Frame = 1 Query: 4 TASFAAALAIGRNGKPFSDCEYLTTALIQCSEHLFNDFKNKREIT 138 +ASFAAALAI +NGK F+D E+ + ++ + L+++F +K +IT Sbjct: 6 SASFAAALAIVKNGKSFTDGEFAKSFMLDVANELYDNFSDKDKIT 50
BLAST of General transcription factor II-I repeat domain-containing protein 2 vs. Ensembl Medaka
Match: ENSORLT00000033718.1 (general transcription factor II-I repeat domain-containing protein 2 [Source:NCBI gene;Acc:110015985]) HSP 1 Score: 54.299 bits (129), Expect = 2.578e-9 Identity = 25/45 (55.56%), Postives = 31/45 (68.89%), Query Frame = 1 Query: 1 TTASFAAALAIGRNGKPFSDCEYLTTALIQCSEHLFNDFKNKREI 135 T SF A I ++GKPF+D EYL I+ S+HLF+DFKNK EI Sbjct: 99 TCTSFVVAQEIVKHGKPFTDGEYLKETFIKISKHLFSDFKNKDEI 143
BLAST of General transcription factor II-I repeat domain-containing protein 2 vs. Ensembl Medaka
Match: ENSORLT00000041272.1 (pep primary_assembly:ASM223467v1:18:30803263:30808674:-1 gene:ENSORLG00000024130.1 transcript:ENSORLT00000041272.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 53.1434 bits (126), Expect = 5.761e-9 Identity = 26/80 (32.50%), Postives = 44/80 (55.00%), Query Frame = 1 Query: 133 ITNCCLELVDLRSSTLWTDKFKTLRGDLEKTEENHL----DMISKCWASLPTKLTCIKKVAFVLLSGFGSSYYCEQRFSQ 360 + + L+ DL++S LW +KFK+L DL++ E +I K W +L +++ + +L+ FGS+ CEQ FS Sbjct: 430 VRDSVLQAADLKASDLWVNKFKSLNEDLDRLERQQAALANQLIVKTWNALTVTFFTLQRGSVAVLTMFGSTSACEQTFSH 509
BLAST of General transcription factor II-I repeat domain-containing protein 2 vs. Planmine SMEST
Match: SMESG000052058.1 (SMESG000052058.1) HSP 1 Score: 49.2914 bits (116), Expect = 4.259e-8 Identity = 26/76 (34.21%), Postives = 44/76 (57.89%), Query Frame = 1 Query: 151 ELVDLRSSTLWTDKFKTLRGDLE-KTEENHLDM-----ISKCWASLPTKLTCIKKVAFVLLSGFGSSYYCEQRFSQ 360 EL++L++S W +KF LR +E + EN + I K W SLP + + +K +A +++ F S+Y+CE F+ Sbjct: 39 ELLELQASLFWREKFVDLRIMVENQLRENVITFNMDGEILKLWKSLPNQFSSLKNLAIAVITVFPSTYFCEFLFAH 114
BLAST of General transcription factor II-I repeat domain-containing protein 2 vs. Planmine SMEST
Match: SMESG000052058.1 (SMESG000052058.1) HSP 1 Score: 48.9062 bits (115), Expect = 5.086e-8 Identity = 26/76 (34.21%), Postives = 44/76 (57.89%), Query Frame = 1 Query: 151 ELVDLRSSTLWTDKFKTLRGDLE-KTEENHLDM-----ISKCWASLPTKLTCIKKVAFVLLSGFGSSYYCEQRFSQ 360 EL++L++S W +KF LR +E + EN + I K W SLP + + +K +A +++ F S+Y+CE F+ Sbjct: 34 ELLELQASLFWREKFVDLRIMVENQLRENVITFNMDGEILKLWKSLPNQFSSLKNLAIAVITVFPSTYFCEFLFAH 109
BLAST of General transcription factor II-I repeat domain-containing protein 2 vs. Planmine SMEST
Match: SMESG000000894.1 (SMESG000000894.1) HSP 1 Score: 42.3578 bits (98), Expect = 7.623e-6 Identity = 26/78 (33.33%), Postives = 40/78 (51.28%), Query Frame = 1 Query: 1 TTASFAAALAIGRNGKPFSDCEYLTTALIQCSEHLFNDFKNKREITNCCLELVDLRSSTLWTDKFKTLRGDLEKTEEN 234 T SF A I + KPF+D EYL ++ S+ L ++FKN+ EI + +L +S KT+R + K +N Sbjct: 42 TPVSFIVANEISKRVKPFTDGEYLKECFMKLSQELVHNFKNRNEIIKM---IQNLPTSA------KTIRDRISKMAQN 110 The following BLAST results are available for this feature:
BLAST of General transcription factor II-I repeat domain-containing protein 2 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of General transcription factor II-I repeat domain-containing protein 2 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of General transcription factor II-I repeat domain-containing protein 2 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of General transcription factor II-I repeat domain-containing protein 2 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 1
BLAST of General transcription factor II-I repeat domain-containing protein 2 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of General transcription factor II-I repeat domain-containing protein 2 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of General transcription factor II-I repeat domain-containing protein 2 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of General transcription factor II-I repeat domain-containing protein 2 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of General transcription factor II-I repeat domain-containing protein 2 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 2
BLAST of General transcription factor II-I repeat domain-containing protein 2 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of General transcription factor II-I repeat domain-containing protein 2 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of General transcription factor II-I repeat domain-containing protein 2 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of General transcription factor II-I repeat domain-containing protein 2 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of General transcription factor II-I repeat domain-containing protein 2 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 3
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30023683 ID=SMED30023683|Name=General transcription factor II-I repeat domain-containing protein 2|organism=Schmidtea mediterranea sexual|type=transcript|length=360bpback to top protein sequence of SMED30023683-orf-1 >SMED30023683-orf-1 ID=SMED30023683-orf-1|Name=SMED30023683-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=120bp TTASFAAALAIGRNGKPFSDCEYLTTALIQCSEHLFNDFKNKREITNCCLback to top Annotated Terms
The following terms have been associated with this transcript:
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