Reverse transcriptase domain-containing protein

Overview
NameReverse transcriptase domain-containing protein
Smed IDSMED30023661
Length (bp)1652
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Reverse transcriptase domain-containing protein (SMED30023661) t-SNE clustered cells

Violin plots show distribution of expression levels for Reverse transcriptase domain-containing protein (SMED30023661) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Reverse transcriptase domain-containing protein (SMED30023661) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Reverse transcriptase domain-containing protein (SMED30023661) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30023661

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 9

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30023661h1SMcG0015791 SmedASXL_007713SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
protonephridiaSMED30023661h1SMcG0015791 dd_Smed_v4_11697_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30023661h1SMcG0015791 dd_Smed_v4_11697_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30023661h1SMcG0015791 dd_Smed_v4_11697_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30023661h1SMcG0015791 dd_Smed_v4_11697_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neuronSMED30023661h1SMcG0015791 dd_Smed_v4_11697_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		whole organism asexual adult colorimetric in situ hybridization evidence
cilated neuronSMED30023661h1SMcG0015791 dd_Smed_v4_11697_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30023661h1SMcG0015791 dd_Smed_v6_11697_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
epidermisSMED30023661h1SMcG0015791 dd_Smed_v4_11697_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Reverse transcriptase domain-containing protein vs. TrEMBL
Match: A0A267F3K6 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig016659g1 PE=4 SV=1)

HSP 1 Score: 167.162 bits (422), Expect = 1.018e-41
Identity = 96/343 (27.99%), Postives = 178/343 (51.90%), Query Frame = 1 
Query:  619 SFIYQQNMPNNSIYPQNTLSENFPPPQPQLNQSENSPQTVTPPPKQIEIHHTFPGNQAQQDFRKLGLAALFCAILDSEYEENSVILSDKFLFNIKIQEIISSIQRHYLSVNQPIYSL-LQSILTNESTKFRVLFT--KGANSTEITRMEQQINNIMTTLVTKISEIMPTTGTLGCESRSILSEIILTNQTFPDNYFYAREFDLIQIDSTNTVRNLGIVEAKLILIGIFYLRALVVTILMKPTEYGLVENVSVVGKDNLMILSSIIVRLYRIILAEYIVQKFPNDSNKKK---TIADSFTELNGSLLTDEEIEPMGKYIKKTIDSCKMLIYKWVNNYVMKLRPA 1629
            SF+ QQ  P  +       ++  P P     +S           ++ ++   FPGN++QQ  R+L   A F A L+    EN       FLF+++++E++ ++ R Y + + P+YS+   +++ +E+ +   L    K  +  E   + Q++   +   V ++++IMPT G LG   +S + E+I +   FP  YFY  E + ++ D       +    A+++LIG+F +RALV T+ +K   YG+V   +   + N+ ++S+++  +YR ++ + +V     D   KK   +I D  +EL+G + TD+EI P+ + +KK++D    L+  W   YV +LR A
Sbjct:  169 SFMQQQRRPPGTPSQPKKPADATPKPATPAKESPKGAAKAESEDEEEQVPFPFPGNKSQQKLRRLAYTAFFLASLNRLTNENKFRPRFDFLFDVRLKEMLETLHRIYKTPDGPLYSVTFATVMGDEAMELTELMNVPKTLSKDEERVVAQELAYCIENTVFQVTDIMPTKGILGTHRKSGVFEMIRSGNLFPKGYFYDMEKNAMEFDDEGRTSRVTDCNARMLLIGLFLMRALVETLYLKTEHYGMVVETTQKAERNIRLVSTLLTYIYRKVVGQLVVM----DQGTKKARVSIPDVPSELSGVMHTDQEIRPVIRVVKKSLDYANNLLMHWSEEYVKRLRGA 507
[Close]
BLAST of Reverse transcriptase domain-containing protein vs. TrEMBL
Match: A0A267GTG7 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig016659g2 PE=4 SV=1)

HSP 1 Score: 161.77 bits (408), Expect = 1.148e-39
Identity = 98/349 (28.08%), Postives = 180/349 (51.58%), Query Frame = 1 
Query:  619 SFIYQQ-----NMPNNSIYPQ-NTLSENFPPPQPQLNQSENSPQTVTPPPKQIEIHHTFPGNQAQQDFRKLGLAALFCAILDSEYEENSVILSDKFLFNIKIQEIISSIQRHYLSVNQPIYSL-LQSILTNESTKFRVLFT--KGANSTEITRMEQQINNIMTTLVTKISEIMPTTGTLGCESRSILSEIILTNQTFPDNYFYAREFDLIQIDSTNTVRNLGIVEAKLILIGIFYLRALVVTILMKPTEYGLVENVSVVGKDNLMILSSIIVRLYRIILAEYIVQKFPNDSNKKK---TIADSFTELNGSLLTDEEIEPMGKYIKKTIDSCKMLIYKWVNNYVMKLRPA 1629
            SF+ QQ       P+ S   Q    ++  P P     +S           ++ ++   FPGN++QQ  R+L   A F A L+    EN       FLF+++++E++ ++ R Y + + P+YS+   +++ +E+ +   L    K  +  E   + Q++   +   V ++++IMPT G LG   +S + E+I +   FP  YFY  E + ++ D       +    A+++LIG+F +RALV T+ +K   YG+V   +   + N+ ++S+++  +YR ++ + +V     D   KK   +I D  +EL+G + TD+EI P+ + +KK++D    L+  W   YV +LR A
Sbjct:  168 SFMQQQQQRPPGTPSRSDRDQPKKPADATPKPATPAKESPKGAAKAESEDEEEQVPFPFPGNKSQQKLRRLAYTAFFLASLNRLTNENKFRPRFDFLFDVRLKEMLETLHRIYKTPDGPLYSVTFATVMGDEAMELTELMNVPKTLSKDEERVVAQELAYCIENTVFQVTDIMPTKGILGTHRKSGVFEMIRSGNLFPKGYFYDMEKNAMEFDDEGRTSRVTDCNARMLLIGLFLMRALVETLYLKTEHYGMVVETTQKAERNIRLVSTLLTYIYRKVVGQLVVM----DQGTKKARVSIPDVPSELSGVMHTDQEIRPVIRVVKKSLDYANNLLMHWSEEYVKRLRGA 512
[Close]
BLAST of Reverse transcriptase domain-containing protein vs. TrEMBL
Match: A0A267ECZ8 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig016659g3 PE=4 SV=1)

HSP 1 Score: 161.77 bits (408), Expect = 1.171e-39
Identity = 99/349 (28.37%), Postives = 179/349 (51.29%), Query Frame = 1 
Query:  619 SFIYQQ-----NMPNNSIYPQ-NTLSENFPPPQPQLNQSENSPQTVTPPPKQIEIHHTFPGNQAQQDFRKLGLAALFCAILDSEYEENSVILSDKFLFNIKIQEIISSIQRHYLSVNQPIYSL-LQSILTNESTKFRVLFT--KGANSTEITRMEQQINNIMTTLVTKISEIMPTTGTLGCESRSILSEIILTNQTFPDNYFYAREFDLIQIDSTNTVRNLGIVEAKLILIGIFYLRALVVTILMKPTEYGLVENVSVVGKDNLMILSSIIVRLYRIILAEYIVQKFPNDSNKKK---TIADSFTELNGSLLTDEEIEPMGKYIKKTIDSCKMLIYKWVNNYVMKLRPA 1629
            SF+ QQ       P+ S   Q    ++  P P     +S           ++ ++   FPGN++QQ  R+L   A F A L+    EN       FLF+++++E++ ++ R Y + + P+YS+   +++ +E+ +   L    K  +  E   + Q++   +   V ++++IMPT G LG   +S + E+I +   FP  YFY  E + +Q D       +    A+++LIG+F +RALV T+ +K   YG+V   +   + N+ ++S+++  +YR ++ + +V     D   KK   +I D  +EL+G + TD+EI P+ +  KK++D    L+  W   YV +LR A
Sbjct:  168 SFMQQQQQRPPGTPSRSDRDQPKKPADATPKPATPAKESPKGAAKAESEDEEEQVPFPFPGNKSQQKLRRLAYTAFFLASLNRLTNENKFRPRFDFLFDVRLKEMLETLHRIYKTPDGPLYSVTFATVMGDEAMELTELMNVPKTLSKDEERVVAQELAYCIENTVFQVTDIMPTKGILGTHRKSGVFEMIRSGNLFPKGYFYDMEKNAMQFDDEGRTSRVTDCNARMLLIGLFLMRALVETLYLKTEHYGMVVETTQKAERNIRLVSTLLTYIYRKVVGQLVVM----DQGTKKARVSIPDVPSELSGVMHTDQEIRPVIRVAKKSLDYANNLLMHWSEEYVKRLRGA 512
[Close]
BLAST of Reverse transcriptase domain-containing protein vs. TrEMBL
Match: A0A1S3HDP7 (relA-associated inhibitor OS=Lingula unguis OX=7574 GN=LOC106154364 PE=4 SV=1)

HSP 1 Score: 160.999 bits (406), Expect = 5.768e-39
Identity = 115/351 (32.76%), Postives = 185/351 (52.71%), Query Frame = 1 
Query:  592 LNNSTNLPPSFIYQQNMPNNSIYPQNTLSENFPPPQPQLNQSENSPQTVTPPPKQIEIHH-TFPGNQAQQDFRKLGLAALFCAILDSEYEENSV-ILSDKFLFNIKIQEIISSIQRHYLSVNQPIYSLLQSILTNESTKFRVLFTKG--ANSTEITRMEQQINNIMTTLVTKISEIMPTTGTLGCESRSILSEIILTNQTFPDNYFYAREFDLIQIDSTNTVRNLGIVEAKLILIGIFYLRALVVTILMKPTEYGLVENV-SVVGKDNLMILSSIIVRLYRIILAEYIVQKFPNDSNKKKTIADSFTELNGSLLTDEEIEPMGKYIKKTIDSCKMLIYKWVNNYVMKLRPA 1629
            +++ T LPP  I  + +P     P  T S   PPP+P+  +   +  T TPP K   +    FPG++  +  R +  AA F A L +E  +N     ++KF+F I+++E ++++ R YL+ +  IY +L S +  E      +  KG    S E  +  Q++  I+  L   I+EIMP TG LG   ++ + E+I   + FP  YF+  E D ++ D  + + N+    A  +LIGIF  R+LV T+LMKP +YGL   V S VG+ NL +L++IIV L R++              +K+T      E++  L TDEE++ +   +KK++D  + L+  W   YV +LR A
Sbjct:  255 IHHVTQLPP--IQTRVLPP----PPRTPSSVTPPPKPKKMREPKA--TPTPPSKPKLVRKIPFPGSRLLRKLRHIFYAAWFIAFLRAEMRKNKGNKPNEKFIFGIQLKEAVAALHRIYLNPDGNIYLIL-SDMVGEGAPDLYVEDKGRFGTSPEDKQNIQELTYIVENLTYHITEIMPATGVLGTHKKAAIFELIKEGKEFPAGYFWQMERDQLEFDENDKITNVTDARAFFLLIGIFLSRSLVTTLLMKPLDYGLSTTVLSEVGERNLKLLATIIVYLIRVVAVP-----------RKQTPLPIPYEISKFLYTDEEMKFILSKLKKSLDYAEGLLRDWGEEYVKRLRAA 585
[Close]
BLAST of Reverse transcriptase domain-containing protein vs. TrEMBL
Match: A0A2R2MKB3 (uncharacterized protein LOC106164852 isoform X1 OS=Lingula unguis OX=7574 GN=LOC106164852 PE=4 SV=1)

HSP 1 Score: 146.747 bits (369), Expect = 6.240e-36
Identity = 96/289 (33.22%), Postives = 157/289 (54.33%), Query Frame = 1 
Query:  775 FPGNQAQQDFRKLGLAALFCAILDSEYEENSV-ILSDKFLFNIKIQEIISSIQRHYLSVNQPIYSLLQSILTNESTKFRVLFTKG--ANSTEITRMEQQINNIMTTLVTKISEIMPTTGTLGCESRSILSEIILTNQTFPDNYFYAREFDLIQIDSTNTVRNLGIVEAKLILIGIFYLRALVVTILMKPTEYGLVENV-SVVGKDNLMILSSIIVRLYRIILAEYIVQKFPNDSNKKKTIADSFTELNGSLLTDEEIEPMGKYIKKTIDSCKMLIYKWVNNYVMKLRPA 1629
            FPG++  +  R +  AA F A L +E  +N     ++KF+F I+++E ++++ R YL+ +  IY +L  ++   +    V   KG    S E  +  Q++  I+  L   I+EIMP TG LG   ++ + E+I   + FP  YF+  E D ++ D  + + N+    A  +LIGIF  R+LV T+LMKP +YGL   V S VG+ NL +L++IIV L R++              +K+T      E++  L TDEE++ +   +KK++D  + L+  W   YV +LR A
Sbjct:   39 FPGSRLLRKLRHIFYAAWFIAFLRAEMRKNKGNKPNEKFIFGIQLKEAVAALHRIYLNPDGNIYLILSDMVGEGAPDLYVE-DKGRFGTSPEDKQNIQELTYIVENLTYHITEIMPATGVLGTHKKAAIFELIKEGKEFPAGYFWQMERDQLEFDENDKITNVTDARAFFLLIGIFLSRSLVTTLLMKPLDYGLSTTVLSEVGERNLKLLATIIVYLIRVVAVP-----------RKQTPLPIPYEISKFLYTDEEMKFILSKLKKSLDYAEGLLRDWGEEYVKRLRAA 315
[Close]
BLAST of Reverse transcriptase domain-containing protein vs. Planmine SMEST
Match: SMESG000042224.1 (SMESG000042224.1)

HSP 1 Score: 934.095 bits (2413), Expect = 0.000e+0
Identity = 502/503 (99.80%), Postives = 503/503 (100.00%), Query Frame = 1 
Query:  121 MSNKLIQEELRDNFYRLKLNILNERLRKEKHKSNLNLIQQTESFVSTDNRNFERDLELKRINLEMDLVKRNHXXXXXXXXXXXXXXXXXXSTKRNQNEISNNRESSKFNQHFEXXXXXXXXXXXXXXXXXXXKSNTEKEIQFVPNYPPMNGMFQSYGLNNSTNLPPSFIYQQNMPNNSIYPQNTLSENFPPPQPQLNQSENSPQTVTPPPKQIEIHHTFPGNQAQQDFRKLGLAALFCAILDSEYEENSVILSDKFLFNIKIQEIISSIQRHYLSVNQPIYSLLQSILTNESTKFRVLFTKGANSTEITRMEQQINNIMTTLVTKISEIMPTTGTLGCESRSILSEIILTNQTFPDNYFYAREFDLIQIDSTNTVRNLGIVEAKLILIGIFYLRALVVTILMKPTEYGLVENVSVVGKDNLMILSSIIVRLYRIILAEYIVQKFPNDSNKKKTIADSFTELNGSLLTDEEIEPMGKYIKKTIDSCKMLIYKWVNNYVMKLRPA 1629
            MSNKLIQEELRDNF+RLKLNILNERLRKEKHKSNLNLIQQTESFVSTDNRNFERDLELKRINLEMDLVKRNHEKLKEKLKLQELEKRLTESTKRNQNEISNNRESSKFNQHFEMMMLQQTQTQNLLMNQFLMKSNTEKEIQFVPNYPPMNGMFQSYGLNNSTNLPPSFIYQQNMPNNSIYPQNTLSENFPPPQPQLNQSENSPQTVTPPPKQIEIHHTFPGNQAQQDFRKLGLAALFCAILDSEYEENSVILSDKFLFNIKIQEIISSIQRHYLSVNQPIYSLLQSILTNESTKFRVLFTKGANSTEITRMEQQINNIMTTLVTKISEIMPTTGTLGCESRSILSEIILTNQTFPDNYFYAREFDLIQIDSTNTVRNLGIVEAKLILIGIFYLRALVVTILMKPTEYGLVENVSVVGKDNLMILSSIIVRLYRIILAEYIVQKFPNDSNKKKTIADSFTELNGSLLTDEEIEPMGKYIKKTIDSCKMLIYKWVNNYVMKLRPA
Sbjct:    1 MSNKLIQEELRDNFFRLKLNILNERLRKEKHKSNLNLIQQTESFVSTDNRNFERDLELKRINLEMDLVKRNHEKLKEKLKLQELEKRLTESTKRNQNEISNNRESSKFNQHFEMMMLQQTQTQNLLMNQFLMKSNTEKEIQFVPNYPPMNGMFQSYGLNNSTNLPPSFIYQQNMPNNSIYPQNTLSENFPPPQPQLNQSENSPQTVTPPPKQIEIHHTFPGNQAQQDFRKLGLAALFCAILDSEYEENSVILSDKFLFNIKIQEIISSIQRHYLSVNQPIYSLLQSILTNESTKFRVLFTKGANSTEITRMEQQINNIMTTLVTKISEIMPTTGTLGCESRSILSEIILTNQTFPDNYFYAREFDLIQIDSTNTVRNLGIVEAKLILIGIFYLRALVVTILMKPTEYGLVENVSVVGKDNLMILSSIIVRLYRIILAEYIVQKFPNDSNKKKTIADSFTELNGSLLTDEEIEPMGKYIKKTIDSCKMLIYKWVNNYVMKLRPA 503
The following BLAST results are available for this feature:
BLAST of Reverse transcriptase domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Reverse transcriptase domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Reverse transcriptase domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Reverse transcriptase domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Reverse transcriptase domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Reverse transcriptase domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Reverse transcriptase domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Reverse transcriptase domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A267F3K61.018e-4127.99Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A267GTG71.148e-3928.08Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A267ECZ81.171e-3928.37Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A1S3HDP75.768e-3932.76relA-associated inhibitor OS=Lingula unguis OX=757... [more]
A0A2R2MKB36.240e-3633.22uncharacterized protein LOC106164852 isoform X1 OS... [more]
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BLAST of Reverse transcriptase domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Reverse transcriptase domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Reverse transcriptase domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Reverse transcriptase domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Reverse transcriptase domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Reverse transcriptase domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000042224.10.000e+099.80SMESG000042224.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30023661 ID=SMED30023661|Name=Reverse transcriptase domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=1652bp
AAAATAAGTGAAAACAAATGCAACACTTAATAATAATCCAACTAACCAAA
TTGGTGATTGTTATTATAAATTTAATCAATATTCAGTTTCAATACGCTGC
TCCGCACAACTTCAATTCAAATGTCGAATAAATTAATTCAAGAAGAATTG
CGAGACAATTTCTATAGATTGAAATTAAATATTCTCAACGAGCGACTACG
GAAGGAAAAACATAAATCAAATTTGAATTTGATTCAACAAACCGAATCGT
TCGTTTCAACAGATAACCGAAACTTTGAACGAGACCTAGAATTGAAGCGA
ATCAATCTTGAAATGGATTTAGTCAAGCGGAATCATGAAAAATTAAAAGA
AAAACTTAAACTTCAAGAATTAGAAAAAAGATTAACTGAATCCACAAAAA
GAAATCAAAATGAAATCTCTAACAATCGGGAAAGTTCTAAATTTAATCAA
CATTTTGAAATGATGATGCTCCAACAAACTCAAACACAAAATTTGTTGAT
GAATCAATTTCTTATGAAATCAAATACAGAAAAAGAAATCCAATTTGTTC
CGAATTATCCTCCGATGAATGGAATGTTTCAATCATACGGACTCAATAAT
TCAACTAATTTGCCTCCATCATTCATATATCAGCAGAATATGCCCAATAA
TTCAATTTATCCACAAAATACACTTAGTGAAAATTTTCCCCCGCCTCAGC
CACAGTTAAACCAATCAGAAAACTCACCACAAACAGTCACACCACCACCG
AAACAAATCGAAATTCATCACACATTTCCGGGAAATCAAGCTCAACAAGA
TTTTCGTAAATTAGGACTGGCGGCGTTATTTTGTGCAATACTCGACTCAG
AGTATGAAGAAAATTCAGTCATTCTAAGTGATAAGTTTTTGTTTAACATT
AAAATTCAAGAGATTATCTCTTCAATTCAACGTCATTATCTATCGGTTAA
TCAACCAATTTATTCATTGCTTCAATCTATACTTACAAATGAGTCAACAA
AATTTCGGGTTCTCTTCACGAAGGGTGCCAACAGCACTGAAATTACCAGA
ATGGAACAACAAATCAATAATATCATGACGACTTTGGTAACAAAAATTTC
AGAAATCATGCCAACCACTGGCACTCTTGGCTGTGAAAGCCGATCAATTC
TTTCTGAAATTATTCTAACCAATCAAACCTTTCCCGATAATTATTTTTAC
GCCCGAGAATTCGATCTCATCCAGATTGATTCGACGAATACAGTTCGAAA
TTTAGGTATTGTTGAAGCAAAATTGATATTAATTGGAATTTTCTATCTAC
GGGCGTTAGTAGTTACAATTCTAATGAAACCGACCGAATACGGTTTGGTG
GAAAATGTAAGCGTTGTGGGAAAAGACAATTTGATGATTTTGTCATCAAT
TATCGTGAGGCTTTATCGGATTATTTTAGCAGAATATATTGTTCAGAAAT
TTCCAAATGATAGCAATAAAAAAAAGACAATAGCTGATAGTTTTACAGAA
TTGAATGGGAGTTTACTGACTGATGAAGAAATAGAACCGATGGGAAAATA
TATTAAAAAGACAATTGATAGTTGCAAAATGTTAATCTATAAATGGGTTA
ACAATTATGTTATGAAATTGAGGCCTGCTTGATTATAGTAGACATTTTCC
AG
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protein sequence of SMED30023661-orf-1

>SMED30023661-orf-1 ID=SMED30023661-orf-1|Name=SMED30023661-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=504bp
MSNKLIQEELRDNFYRLKLNILNERLRKEKHKSNLNLIQQTESFVSTDNR
NFERDLELKRINLEMDLVKRNHEKLKEKLKLQELEKRLTESTKRNQNEIS
NNRESSKFNQHFEMMMLQQTQTQNLLMNQFLMKSNTEKEIQFVPNYPPMN
GMFQSYGLNNSTNLPPSFIYQQNMPNNSIYPQNTLSENFPPPQPQLNQSE
NSPQTVTPPPKQIEIHHTFPGNQAQQDFRKLGLAALFCAILDSEYEENSV
ILSDKFLFNIKIQEIISSIQRHYLSVNQPIYSLLQSILTNESTKFRVLFT
KGANSTEITRMEQQINNIMTTLVTKISEIMPTTGTLGCESRSILSEIILT
NQTFPDNYFYAREFDLIQIDSTNTVRNLGIVEAKLILIGIFYLRALVVTI
LMKPTEYGLVENVSVVGKDNLMILSSIIVRLYRIILAEYIVQKFPNDSNK
KKTIADSFTELNGSLLTDEEIEPMGKYIKKTIDSCKMLIYKWVNNYVMKL
RPA*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000020protonephridia
PLANA:0000034epidermis
PLANA:0000099neuron
PLANA:0000418head
PLANA:0002032epidermal cell
Vocabulary: INTERPRO
TermDefinition
IPR038915PRR29-like
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 58..101
NoneNo IPR availablePANTHERPTHR28604:SF3coord: 368..500
coord: 201..330
IPR038915Proline-rich protein 29-likePANTHERPTHR28604FAMILY NOT NAMEDcoord: 368..500
coord: 201..330