SMED30023645

Overview


Name	SMED30023645
Smed ID	SMED30023645
Length (bp)	1479

Neoblast Clusters

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30023645 (SMED30023645) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30023645 (SMED30023645) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30023645

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 4

Expressed In	Reference Transcript	Gene Models	Published Transcript	Transcriptome	Publication
head region	SMED30023645	SMESG000077254.1	SmedASXL_011159	SmedAsxl_ww_GCZZ01	PMID:27034770 Currie et al., 2016
muscle cell	SMED30023645	SMESG000077254.1	dd_Smed_v4_16969_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
head region	SMED30023645	SMESG000077254.1	dd_Smed_v6_16969_0_1	dd_Smed_v6	PMID:28171748 Stückemann et al., 2017
enteric muscle cell	SMED30023645	SMESG000077254.1	dd_Smed_v4_16969_0_1	dd_Smed_v4	PMID:30471994 Scimone et al., 2018

Note: Hover over icons to view figure legend

Alignments

SMED30023645 aligns in the following genomic locations:


Alignment Location	Alignment Score
v31.011334:14276..15671 -	6024

Homology

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BLAST of SMED30023645 vs. Ensembl Human
Match: HTR2A (5-hydroxytryptamine receptor 2A [Source:HGNC Symbol;Acc:HGNC:5293])

HSP 1 Score: 62.003 bits (149), Expect = 1.144e-9
Identity = 49/192 (25.52%), Postives = 96/192 (50.00%), Query Frame = -2

Query:  801 NIIIMVLIRKKRSIEDIKYHYIRSLCISNMILATFNIPVSIIEDFTLLNEQR----TTVCFISVYLDSFLCNQIMLLLLCNTIDCFFRIRNSQLYKLSNRPIPFYLKYFAPWXXXXXXXXXXXXISVQYKCEIINH-VCVLADPNFIIIRSILGFAIPLITSAIFIFITAKVL--------SDIRSKLDLKS 1337
            NI++++ +  ++ +++   +++ SL I++M+L    +PVS++   T+L   R    + +C + +YLD       ++ L   ++D +  I+N   +   N     +LK  A W I +  ++ +    +Q   ++     C+LAD NF++I S + F IPL    I  F+T K L        SD+ ++  L S
Sbjct:   92 NILVIMAVSLEKKLQNATNYFLMSLAIADMLLGFLVMPVSML---TILYGYRWPLPSKLCAVWIYLDVLFSTASIMHLCAISLDRYVAIQNPIHHSRFNSRTKAFLKIIAVWTISVGISMPIPVFGLQDDSKVFKEGSCLLADDNFVLIGSFVSFFIPLTIMVITYFLTIKSLQKEATLCVSDLGTRAKLAS 280

[Close]

Query:  801 NIIIMVLIRKKRSIEDIKYHYIRSLCISNMILATFNIPVSIIEDFTLLNEQR----TTVCFISVYLDSFLCNQIMLLLLCNTIDCFFRIRNSQLYKLSNRPIPFYLKYFAPWXXXXXXXXXXXXISVQYKCEIINH-VCVLADPNFIIIRSILGFAIPLITSAIFIFITAKVL--------SDIRSKLDLKS 1337
            NI++++ +  ++ +++   +++ SL I++M+L    +PVS++   T+L   R    + +C + +YLD       ++ L   ++D +  I+N   +   N     +LK  A W I +  ++ +    +Q   ++     C+LAD NF++I S + F IPL    I  F+T K L        SD+ ++  L S
Sbjct:   92 NILVIMAVSLEKKLQNATNYFLMSLAIADMLLGFLVMPVSML---TILYGYRWPLPSKLCAVWIYLDVLFSTASIMHLCAISLDRYVAIQNPIHHSRFNSRTKAFLKIIAVWTISVGISMPIPVFGLQDDSKVFKEGSCLLADDNFVLIGSFVSFFIPLTIMVITYFLTIKSLQKEATLCVSDLGTRAKLAS 280

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BLAST of SMED30023645 vs. Ensembl Celegans
Match: ser-1 (SERotonin/octopamine receptor family; Serotonin receptor [Source:UniProtKB/TrEMBL;Acc:O17470])

HSP 1 Score: 50.0618 bits (118), Expect = 3.587e-6
Identity = 53/237 (22.36%), Postives = 110/237 (46.41%), Query Frame = -2

Query:  732 ITYSNNKIKFPLDLICAVGIMFCAWILNIIIMVLIRKKRSIEDIKYHYIRSLCISNMILATFNIPVSII-EDFTLLNEQRTTVCFISVYLDSFLCNQIMLLLLCNTIDCFFRIRNSQLYKLSNRPIPF-YLKYFAPWXXXXXXXXXXXXISVQYKCEII-NHVCVLADPNFIIIRSILGFAIPLITSAIFIFITAKVLSDIRSKLDLKSDKDFEN-----ILPEIPLFSVPQYRCTV 1418
            I + N    F L ++C +G+     I N ++ V I   R + ++  +++ SL ++++++    +P+SI+ E    +     ++C + VY D FLC+  ++ +   ++D +  I  SQ  +  NR     ++K    W++ ++ +  +  +++     I+ N+ C++    +II  S + F IPL    +    T ++L+   S L  K+   F        +P   L     Y  TV
Sbjct:    3 IYHFNGVALFLLPVLCLIGL-----IGNFLVCVAIATDRRLHNVTNYFLFSLALADLLVCCIVMPLSIVVEVRHGVWTWSVSMCLLYVYSDVFLCSASIVHMSVISLDRYLGI--SQPLRTRNRSKTLIFIKIAIVWVVTLLVSCPIAVLAMHDTANILRNNQCMIFSRYYIIYGSTMTFLIPLCIMGVTYAKTTQLLNKQASILSQKAGDKFNGNGLRRTMPHRKLGYARTYSATV 232

[Close]

BLAST of SMED30023645 vs. Ensembl Celegans
Match: ser-1 (SERotonin/octopamine receptor family; Serotonin receptor [Source:UniProtKB/TrEMBL;Acc:O17470])

HSP 1 Score: 50.0618 bits (118), Expect = 3.657e-6
Identity = 54/243 (22.22%), Postives = 111/243 (45.68%), Query Frame = -2

Query:  714 ITYSNNKIKFPLDLICAVGIMFCAWILNIIIMVLIRKKRSIEDIKYHYIRSLCISNMILATFNIPVSII-EDFTLLNEQRTTVCFISVYLDSFLCNQIMLLLLCNTIDCFFRIRNSQLYKLSNRPIPF-YLKYFAPWXXXXXXXXXXXXISVQYKCEII-NHVCVLADPNFIIIRSILGFAIPLITSAIFIFITAKVLSDIRSKLDLKSDKDFEN-----ILPEIPLFSVPQYRCTVGTANEN 1418
            I + N    F L ++C +G+     I N ++ V I   R + ++  +++ SL ++++++    +P+SI+ E    +     ++C + VY D FLC+  ++ +   ++D +  I  SQ  +  NR     ++K    W++ ++ +  +  +++     I+ N+ C++    +II  S + F IPL    +    T ++L+   S L  K+   F        +P   L     Y  TV     N
Sbjct:   51 IYHFNGVALFLLPVLCLIGL-----IGNFLVCVAIATDRRLHNVTNYFLFSLALADLLVCCIVMPLSIVVEVRHGVWTWSVSMCLLYVYSDVFLCSASIVHMSVISLDRYLGI--SQPLRTRNRSKTLIFIKIAIVWVVTLLVSCPIAVLAMHDTANILRNNQCMIFSRYYIIYGSTMTFLIPLCIMGVTYAKTTQLLNKQASILSQKAGDKFNGNGLRRTMPHRKLGYARTYSATVNGTIAN 286

[Close]

BLAST of SMED30023645 vs. Ensembl Zebrafish
Match: htr2ab (5-hydroxytryptamine (serotonin) receptor 2A, genome duplicate b [Source:ZFIN;Acc:ZDB-GENE-120215-60])

HSP 1 Score: 63.1586 bits (152), Expect = 2.856e-10
Identity = 45/173 (26.01%), Postives = 89/173 (51.45%), Query Frame = -2

Query:  834 NIIIMVLIRKKRSIEDIKYHYIRSLCISNMILATFNIPVSIIEDFTLLNEQR----TTVCFISVYLDSFLCNQIMLLLLCNTIDCFFRIRNSQLYKLSNRPIPFYLKYFAPWXXXXXXXXXXXXISVQYKCEII-NHVCVLADPNFIIIRSILGFAIPLITSAIFIFITAKVL 1337
            NI++++ +  +R +++   +++RSL I++M+L    +PV+++   T+L         T+C I +YLD       ++ L   ++D +  IRN   +  SN      +K  A W I ++ ++ +  + +    ++  N  C L D NF++I S + F +PLI   +  F+T   L
Sbjct:   79 NILVIMAVSLERKLQNATNYFLRSLAITDMLLGILVMPVAMV---TILYGYTWPLPRTLCPIWIYLDVLFSTASIMHLCAISLDRYIAIRNPIHHSRSNSLTKARVKIIAAWTISVVISMPVPVLGLHDHSKVFRNESCQLTDNNFVLIGSFVAFFVPLIIMVVTYFLTISAL 248

[Close]

BLAST of SMED30023645 vs. Ensembl Zebrafish
Match: drd2b (dopamine receptor D2b [Source:ZFIN;Acc:ZDB-GENE-030910-2])

HSP 1 Score: 62.003 bits (149), Expect = 7.967e-10
Identity = 92/439 (20.96%), Postives = 181/439 (41.23%), Query Frame = -2

Query:   99 NIIIMVLIRKKRSIEDIKYHYIRSLCISNMILATFNIPVSIIEDFTLLNEQRTTV--CFISVYLDSFLCNQIMLLLLCNTIDCFFRIRNSQLYKL---SNRPIPFYLKYFAPWXXXXXXXXXXXXISVQYKCEII----------NHVCVLADPNFIIIRSILGFAIPLITSAIFIFITAKVLSDIRSKLDLKSDKDFENILPEIPLFSVPQYRCTVGTANENIRLRQLDFET---VPEET-EAKLVQHHCPQHGLL-------TFPLQEKSGNLFFEPSSVVYQCSNQPTSSSKHSPDIVLSSNQDFTKPISMLHQDQPKESQTPSAAINQEILQCADKEFTINKQNFNKRIEEFRQERKXXXXXXXXXXXXIMFWGPYITSTLIQGICILPNCPLSISIPKINEFKWLSYSSSVIIPLFILVMDNRVRRTFCYILRC 1337
            N+++ + + ++++++    + I SL ++++++AT  +P  +     ++ E R +   C I V LD  +C   +L L   +ID +  +    LY     S R +   +     W++           S    C ++            VC +A+P F++  SI+ F +P I + +       VL   R +++ K      +   + PL    + +C   T  E+++L  +  +T   VP +  +A+L++    Q G +       T P ++K        SS+V      P S +  SP      +    K         PKE++     +  + L       T+ K   +KR     +E+K + +   +  + I+ W P+  + +++  C     PL +     N F WL Y +S + P+     +   R+ F  IL C
Sbjct:   50 NVLVCMAVSREKALQTTTNYLIVSLAVADLLVATLVMPWVVY--LEVVGEWRFSKIHCDIFVTLDVMMCTASILNLCAISIDRYTAVAMPMLYNTRYSSKRRVTVMISVV--WVL-----------SFAISCPLLFGLNNTATRDGSVCEIANPAFVVYSSIMSFYVPFIITLLVYVQIYVVLRKRRKRVNTKRSCQKTDADAQPPL----KEKC---THPEDVKLCTVIIKTNGGVPAKNKKAQLIKEVLHQGGDVGMEMVAGTSPPEKKK-----LASSLVVDLLATPPSPNHGSPSHAEYQSNGDEKNGHAKDVQSPKETK----PVETQALPNGKTRTTVTK-TMSKRKMSQHKEKKATQMLAIVLGVFIICWLPFFITHILKTHCTSCVVPLEM----YNAFTWLGYVNSAVNPIIYTTFNVEFRKAFIKILHC 452

[Close]

Query:   99 NIIIMVLIRKKRSIEDIKYHYIRSLCISNMILATFNIPVSIIEDFTLLNEQRTTV--CFISVYLDSFLCNQIMLLLLCNTIDCFFRIRNSQLYKL---SNRPIPFYLKYFAPWXXXXXXXXXXXXISVQYKCEII----------NHVCVLADPNFIIIRSILGFAIPLITSAIFIFITAKVLSDIRSKLDLKSDKDFENILPEIPLFSVPQYRCTVGTANENIRLRQLDFET---VPEET-EAKLVQHHCPQHGLL-------TFPLQEKSGNLFFEPSSVVYQCSNQPTSSSKHSPDIVLSSNQDFTKPISMLHQDQPKESQTPSAAINQEILQCADKEFTINKQNFNKRIEEFRQERKXXXXXXXXXXXXIMFWGPYITSTLIQGICILPNCPLSISIPKINEFKWLSYSSSVIIPLFILVMDNRVRRTFCYILRC 1337
            N+++ + + ++++++    + I SL ++++++AT  +P  +     ++ E R +   C I V LD  +C   +L L   +ID +  +    LY     S R +   +     W++           S    C ++            VC +A+P F++  SI+ F +P I + +       VL   R +++ K      +   + PL    + +C   T  E+++L  +  +T   VP +  +A+L++    Q G +       T P ++K        SS+V      P S +  SP      +    K         PKE++     +  + L       T+ K   +KR     +E+K + +   +  + I+ W P+  + +++  C     PL +     N F WL Y +S + P+     +   R+ F  IL C
Sbjct:   50 NVLVCMAVSREKALQTTTNYLIVSLAVADLLVATLVMPWVVY--LEVVGEWRFSKIHCDIFVTLDVMMCTASILNLCAISIDRYTAVAMPMLYNTRYSSKRRVTVMISVV--WVL-----------SFAISCPLLFGLNNTATRDGSVCEIANPAFVVYSSIMSFYVPFIITLLVYVQIYVVLRKRRKRVNTKRSCQKTDADAQPPL----KEKC---THPEDVKLCTVIIKTNGGVPAKNKKAQLIKEVLHQGGDVGMEMVAGTSPPEKKK-----LASSLVVDLLATPPSPNHGSPSHAEYQSNGDEKNGHAKDVQSPKETK----PVETQALPNGKTRTTVTK-TMSKRKMSQHKEKKATQMLAIVLGVFIICWLPFFITHILKTHCTSCVVPLEM----YNAFTWLGYVNSAVNPIIYTTFNVEFRKAFIKILHC 452

[Close]

Query:   99 NIIIMVLIRKKRSIEDIKYHYIRSLCISNMILATFNIPVSIIEDFTLLNEQRTTV--CFISVYLDSFLCNQIMLLLLCNTIDCFFRIRNSQLYKL---SNRPIPFYLKYFAPWXXXXXXXXXXXXISVQYKCEII----------NHVCVLADPNFIIIRSILGFAIPLITSAIFIFITAKVLSDIRSKLDLKSDKDFENILPEIPLFSVPQYRCTVGTANENIRLRQLDFET---VPEET-EAKLVQHHCPQHGLL-------TFPLQEKSGNLFFEPSSVVYQCSNQPTSSSKHSPDIVLSSNQDFTKPISMLHQDQPKESQTPSAAINQEILQCADKEFTINKQNFNKRIEEFRQERKXXXXXXXXXXXXIMFWGPYITSTLIQGICILPNCPLSISIPKINEFKWLSYSSSVIIPLFILVMDNRVRRTFCYILRC 1337
            N+++ + + ++++++    + I SL ++++++AT  +P  +     ++ E R +   C I V LD  +C   +L L   +ID +  +    LY     S R +   +     W++           S    C ++            VC +A+P F++  SI+ F +P I + +       VL   R +++ K      +   + PL    + +C   T  E+++L  +  +T   VP +  +A+L++    Q G +       T P ++K        SS+V      P S +  SP      +    K         PKE++     +  + L       T+ K   +KR     +E+K + +   +  + I+ W P+  + +++  C     PL +     N F WL Y +S + P+     +   R+ F  IL C
Sbjct:   50 NVLVCMAVSREKALQTTTNYLIVSLAVADLLVATLVMPWVVY--LEVVGEWRFSKIHCDIFVTLDVMMCTASILNLCAISIDRYTAVAMPMLYNTRYSSKRRVTVMISVV--WVL-----------SFAISCPLLFGLNNTATRDGSVCEIANPAFVVYSSIMSFYVPFIITLLVYVQIYVVLRKRRKRVNTKRSCQKTDADAQPPL----KEKC---THPEDVKLCTVIIKTNGGVPAKNKKAQLIKEVLHQGGDVGMEMVAGTSPPEKKK-----LASSLVVDLLATPPSPNHGSPSHAEYQSNGDEKNGHAKDVQSPKETK----PVETQALPNGKTRTTVTK-TMSKRKMSQHKEKKATQMLAIVLGVFIICWLPFFITHILKTHCTSCVVPLEM----YNAFTWLGYVNSAVNPIIYTTFNVEFRKAFIKILHC 452

[Close]

BLAST of SMED30023645 vs. Ensembl Zebrafish
Match: htr2cl1 (5-hydroxytryptamine (serotonin) receptor 2C, G protein-coupled-like 1 [Source:ZFIN;Acc:ZDB-GENE-081104-48])

HSP 1 Score: 52.7582 bits (125), Expect = 6.278e-7
Identity = 40/172 (23.26%), Postives = 87/172 (50.58%), Query Frame = -2

Query:  834 NIIIMVLIRKKRSIEDIKYHYIRSLCISNMILATFNIPVSIIE---DFTLLNEQRTTVCFISVYLDSFLCNQIMLLLLCNTIDCFFRIRNSQLYKLSNRPIPFYLKYFAPWXXXXXXXXXXXXISVQYKCEII-NHVCVLADPNFIIIRSILGFAIPLITSAIFIFITAKVL 1337
            NI++++ +  ++ +++    ++RSL +++M++    +P+S+I    D+       + +C I +YLD       ++ L   ++D +  IRN   +   N      LK  A W I I  ++ +  I +  + +++ N +C L +  FI++ S + F IPL+   +   +T + L
Sbjct:   85 NILVILAVSLEKKLQNATNFFLRSLAVADMLVGILVMPISLINILYDYAW--PLPSALCPIWIYLDVLFSTASIMHLCAISLDRYVAIRNPIEHSRFNSRTKAMLKIAAVWTISIGISMPIPVIGLSNREKVLKNGICALNEELFILVGSFVAFFIPLVIMVVTYCLTVQAL 254

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BLAST of SMED30023645 vs. Ensembl Xenopus
Match: slc25a29 (solute carrier family 25 (mitochondrial carnitine/acylcarnitine carrier), member 29 [Source:Xenbase;Acc:XB-GENE-5763797])

HSP 1 Score: 60.077 bits (144), Expect = 5.056e-9
Identity = 46/173 (26.59%), Postives = 87/173 (50.29%), Query Frame = -2

Query:  834 NIIIMVLIRKKRSIEDIKYHYIRSLCISNMILATFNIPVSIIEDFTLLNEQ----RTTVCFISVYLDSFLCNQIMLLLLCNTIDCFFRIRNSQLYKLSNRPIPFYLKYFAPWXXXXXXXXXXXXISVQYKCEIINHV-CVLADPNFIIIRSILGFAIPLITSAIFIFITAKVL 1337
            NI++++ +  ++ +++   +++ SL I++M+L    +PVS++    +LN+        +C + +YLD       ++ L   ++D +  IRN   +   N       K  A W I +  ++ +    +Q   ++ +   C L+D NFIII S + F IPLI   I  F+T K L
Sbjct:   85 NILVIMAVSLEKKLQNATNYFLMSLAIADMLLGFLVMPVSMLN---ILNDYEWPLHRKLCPVWIYLDVLFSTASIMHLCAISLDRYIAIRNPIHHSRFNSRTKALAKIIAVWTISVGISMPVPVFGLQDDSKVFDQENCSLSDENFIIIGSFVAFFIPLIIMVITYFLTIKSL 254

[Close]

BLAST of SMED30023645 vs. Ensembl Xenopus
Match: slc25a29 (solute carrier family 25 (mitochondrial carnitine/acylcarnitine carrier), member 29 [Source:Xenbase;Acc:XB-GENE-5763797])

HSP 1 Score: 59.6918 bits (143), Expect = 5.170e-9
Identity = 46/173 (26.59%), Postives = 87/173 (50.29%), Query Frame = -2

Query:  834 NIIIMVLIRKKRSIEDIKYHYIRSLCISNMILATFNIPVSIIEDFTLLNEQ----RTTVCFISVYLDSFLCNQIMLLLLCNTIDCFFRIRNSQLYKLSNRPIPFYLKYFAPWXXXXXXXXXXXXISVQYKCEIINHV-CVLADPNFIIIRSILGFAIPLITSAIFIFITAKVL 1337
            NI++++ +  ++ +++   +++ SL I++M+L    +PVS++    +LN+        +C + +YLD       ++ L   ++D +  IRN   +   N       K  A W I +  ++ +    +Q   ++ +   C L+D NFIII S + F IPLI   I  F+T K L
Sbjct:   83 NILVIMAVSLEKKLQNATNYFLMSLAIADMLLGFLVMPVSMLN---ILNDYEWPLHRKLCPVWIYLDVLFSTASIMHLCAISLDRYIAIRNPIHHSRFNSRTKALAKIIAVWTISVGISMPVPVFGLQDDSKVFDQENCSLSDENFIIIGSFVAFFIPLIIMVITYFLTIKSL 252

[Close]

BLAST of SMED30023645 vs. Ensembl Xenopus
Match: slc25a29 (solute carrier family 25 (mitochondrial carnitine/acylcarnitine carrier), member 29 [Source:Xenbase;Acc:XB-GENE-5763797])

HSP 1 Score: 59.6918 bits (143), Expect = 5.277e-9
Identity = 46/173 (26.59%), Postives = 87/173 (50.29%), Query Frame = -2

Query:  834 NIIIMVLIRKKRSIEDIKYHYIRSLCISNMILATFNIPVSIIEDFTLLNEQ----RTTVCFISVYLDSFLCNQIMLLLLCNTIDCFFRIRNSQLYKLSNRPIPFYLKYFAPWXXXXXXXXXXXXISVQYKCEIINHV-CVLADPNFIIIRSILGFAIPLITSAIFIFITAKVL 1337
            NI++++ +  ++ +++   +++ SL I++M+L    +PVS++    +LN+        +C + +YLD       ++ L   ++D +  IRN   +   N       K  A W I +  ++ +    +Q   ++ +   C L+D NFIII S + F IPLI   I  F+T K L
Sbjct:   76 NILVIMAVSLEKKLQNATNYFLMSLAIADMLLGFLVMPVSMLN---ILNDYEWPLHRKLCPVWIYLDVLFSTASIMHLCAISLDRYIAIRNPIHHSRFNSRTKALAKIIAVWTISVGISMPVPVFGLQDDSKVFDQENCSLSDENFIIIGSFVAFFIPLIIMVITYFLTIKSL 245

[Close]

BLAST of SMED30023645 vs. Ensembl Xenopus
Match: slc25a29 (solute carrier family 25 (mitochondrial carnitine/acylcarnitine carrier), member 29 [Source:Xenbase;Acc:XB-GENE-5763797])

HSP 1 Score: 59.6918 bits (143), Expect = 6.774e-9
Identity = 46/173 (26.59%), Postives = 87/173 (50.29%), Query Frame = -2

Query:  834 NIIIMVLIRKKRSIEDIKYHYIRSLCISNMILATFNIPVSIIEDFTLLNEQ----RTTVCFISVYLDSFLCNQIMLLLLCNTIDCFFRIRNSQLYKLSNRPIPFYLKYFAPWXXXXXXXXXXXXISVQYKCEIINHV-CVLADPNFIIIRSILGFAIPLITSAIFIFITAKVL 1337
            NI++++ +  ++ +++   +++ SL I++M+L    +PVS++    +LN+        +C + +YLD       ++ L   ++D +  IRN   +   N       K  A W I +  ++ +    +Q   ++ +   C L+D NFIII S + F IPLI   I  F+T K L
Sbjct:  105 NILVIMAVSLEKKLQNATNYFLMSLAIADMLLGFLVMPVSMLN---ILNDYEWPLHRKLCPVWIYLDVLFSTASIMHLCAISLDRYIAIRNPIHHSRFNSRTKALAKIIAVWTISVGISMPVPVFGLQDDSKVFDQENCSLSDENFIIIGSFVAFFIPLIIMVITYFLTIKSL 274

[Close]

BLAST of SMED30023645 vs. Ensembl Xenopus
Match: DRD2 (dopamine receptor D2 [Source:NCBI gene;Acc:100493063])

HSP 1 Score: 56.9954 bits (136), Expect = 3.870e-8
Identity = 95/480 (19.79%), Postives = 185/480 (38.54%), Query Frame = -2

Query:   99 NQSTTITYSNNKIKFPLDLICAVGIMFCAWILNIIIMVLIRKKRSIEDIKYHYIRSLCISNMILATFNIPVSIIEDFTLLNEQRTTV--CFISVYLDSFLCNQIMLLLLCNTIDCFFRIRNSQLYKL---SNRPIPFYLKYFAPWXXXXXXXXXXXXISVQYKCEII-------NHVCVLADPNFIIIRSILGFAIPLITSAIFIFITAKVLSDIRSKLDLK---------SDKDFENILPEIPLFSV--------PQYRCTV----GTANENIRLRQLDFETVPEETEAKLVQHHCPQHGLLTFPLQEKSGNLFFEPSSVVYQCSNQPTSSSKHSPDIVLSSNQDFTKPISMLHQDQPKESQTPSAAINQEILQCADKEFTINKQNFNKRIEEFRQERKXXXXXXXXXXXXIMFWGPYITSTLIQGICILPNCPLSISIPKI--NEFKWLSYSSSVIIPLFILVMDNRVRRTFCYILRC 1433
            N  T  T  + K  +    +    ++F     N+++ + + ++++++    + I SL ++++++AT  +P  +  +  ++ E R +   C I V LD  +C   +L L   +ID +  +    LY     S R +   +     W++           S    C ++       + VC++ +P F+I  SI+ F +P I + +       VL   R +++ K         + KD      ++ L +V        P  +  V    G   E++ +  +   + PE+T+ K      P H  L  P                 QC+N    S   SP                     PK+S  P+     + +       +I  +  +KR     +E+K + +   +  + I+ W P+    ++   C   NC    +IP+   + F WL Y +S + P+     +   R+ F  IL C
Sbjct:   14 NNGTNGTAVDQKPHYNYYAMLLTLLIFVIVFGNVLVCIAVSREKALQTTTNYLIVSLAVADLLVATLVMPWVVYME--VVGEWRFSRIHCDIFVTLDVMMCTASILNLCAISIDRYTAVAMPMLYNTRYSSKRRVTVMISVV--WVL-----------SFAISCPLLFGLNNTASKVCIIDNPAFVIYSSIVSFYVPFIVTLLVYVQIYIVLRKRRKRVNTKRNSHGVEVNAHKDKCTHPEDVKLCAVFVKSNGSFPTDKKKVVSEAGNHPEDMDVEMMSSTSPPEKTKHKSAS---PNHHQLAVPATSN-------------QCNNANLPSPVDSP-------------YKAEKNGHPKDSTKPAKVFEIQTMPNGKTRTSI--KTMSKRKLSQHKEKKATQMLAIVLGVFIICWLPFFIIHILNMHC---NC----NIPQALYSAFTWLGYVNSAVNPIIYTTFNVEFRKAFIKILHC 440

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BLAST of SMED30023645 vs. Ensembl Mouse
Match: Htr2a (5-hydroxytryptamine (serotonin) receptor 2A [Source:MGI Symbol;Acc:MGI:109521])

HSP 1 Score: 62.7734 bits (151), Expect = 4.474e-10
Identity = 49/192 (25.52%), Postives = 96/192 (50.00%), Query Frame = -2

Query:  801 NIIIMVLIRKKRSIEDIKYHYIRSLCISNMILATFNIPVSIIEDFTLLNEQR----TTVCFISVYLDSFLCNQIMLLLLCNTIDCFFRIRNSQLYKLSNRPIPFYLKYFAPWXXXXXXXXXXXXISVQYKCEIINH-VCVLADPNFIIIRSILGFAIPLITSAIFIFITAKVL--------SDIRSKLDLKS 1337
            NI++++ +  ++ +++   +++ SL I++M+L    +PVS++   T+L   R    + +C + +YLD       ++ L   ++D +  I+N   +   N     +LK  A W I +  ++ +    +Q   ++     C+LAD NF++I S + F IPL    I  F+T K L        SD+ ++  L S
Sbjct:   92 NILVIMAVSLEKKLQNATNYFLMSLAIADMLLGFLVMPVSML---TILYGYRWPLPSKLCAVWIYLDVLFSTASIMHLCAISLDRYVAIQNPIHHSRFNSRTKAFLKIIAVWTISVGISMPIPVFGLQDDSKVFKEGSCLLADDNFVLIGSFVAFFIPLTIMVITYFLTIKSLQKEATLCVSDLSTRAKLSS 280

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BLAST of SMED30023645 vs. Ensembl Mouse
Match: Htr2c (5-hydroxytryptamine (serotonin) receptor 2C [Source:MGI Symbol;Acc:MGI:96281])

HSP 1 Score: 50.447 bits (119), Expect = 3.265e-6
Identity = 41/171 (23.98%), Postives = 85/171 (49.71%), Query Frame = -2

Query:  834 NIIIMVLIRKKRSIEDIKYHYIRSLCISNMILATFNIPVSIIED-FTLLNEQRTTVCFISVYLDSFLCNQIMLLLLCNTIDCFFRIRNSQLYKLSNRPIPFYLKYFAPWXXXXXXXXXXXXISVQYKCEII--NHVCVLADPNFIIIRSILGFAIPLITSAIFIFITAKVL 1337
            NI++++ +  ++ + +   +++ SL I++M++    +P+S++   +  +      +C + + LD       ++ L   ++D +  IRN   +   N      +K    W I I  ++ +  I ++ + ++   N  CVL DPNF++I S + F IPL    I  F+T  VL
Sbjct:   72 NILVIMAVSMEKKLHNATNYFLMSLAIADMLVGLLVMPLSLLAILYDYVWPLPRYLCPVWISLDVLFSTASIMHLCAISLDRYVAIRNPIEHSRFNSRTKAIMKIAIVWAISIGVSVPIPVIGLRDESKVFVNNTTCVLNDPNFVLIGSFVAFFIPLTIMVITYFLTIYVL 242

[Close]

Query:  834 NIIIMVLIRKKRSIEDIKYHYIRSLCISNMILATFNIPVSIIED-FTLLNEQRTTVCFISVYLDSFLCNQIMLLLLCNTIDCFFRIRNSQLYKLSNRPIPFYLKYFAPWXXXXXXXXXXXXISVQYKCEII--NHVCVLADPNFIIIRSILGFAIPLITSAIFIFITAKVL 1337
            NI++++ +  ++ + +   +++ SL I++M++    +P+S++   +  +      +C + + LD       ++ L   ++D +  IRN   +   N      +K    W I I  ++ +  I ++ + ++   N  CVL DPNF++I S + F IPL    I  F+T  VL
Sbjct:   72 NILVIMAVSMEKKLHNATNYFLMSLAIADMLVGLLVMPLSLLAILYDYVWPLPRYLCPVWISLDVLFSTASIMHLCAISLDRYVAIRNPIEHSRFNSRTKAIMKIAIVWAISIGVSVPIPVIGLRDESKVFVNNTTCVLNDPNFVLIGSFVAFFIPLTIMVITYFLTIYVL 242

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BLAST of SMED30023645 vs. UniProt/SwissProt
Match: sp|P50129|5HT2A_PIG (5-hydroxytryptamine receptor 2A OS=Sus scrofa OX=9823 GN=HTR2A PE=2 SV=1)

HSP 1 Score: 63.1586 bits (152), Expect = 2.235e-9
Identity = 49/192 (25.52%), Postives = 97/192 (50.52%), Query Frame = -2

Query:  801 NIIIMVLIRKKRSIEDIKYHYIRSLCISNMILATFNIPVSIIEDFTLLNEQR----TTVCFISVYLDSFLCNQIMLLLLCNTIDCFFRIRNSQLYKLSNRPIPFYLKYFAPWXXXXXXXXXXXXISVQYKCEIINH-VCVLADPNFIIIRSILGFAIPLITSAIFIFITAKVL--------SDIRSKLDLKS 1337
            NI++++ +  ++ +++   +++ SL I++M+L    +PVS++   T+L   R    + +C + +YLD       ++ L   ++D +  I+N   ++  N     +LK  A W I +  ++ +    +Q   ++     C+LAD NF++I S + F IPL    I  F+T K L        SD+ ++  L S
Sbjct:   92 NILVIMAVSLEKKLQNATNYFLMSLAIADMLLGFLVMPVSML---TILYGYRWPLPSKLCAVWIYLDVLFSTASIMHLCAISLDRYVAIQNPIHHRRFNSRTKAFLKIIAVWTISVGISMPIPVFGLQDDSKVFKEGSCLLADDNFVLIGSFVSFFIPLTIMVITYFLTIKSLQKEATLCVSDLGTRAKLAS 280

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BLAST of SMED30023645 vs. UniProt/SwissProt
Match: sp|P18599|5HT2A_CRIGR (5-hydroxytryptamine receptor 2A OS=Cricetulus griseus OX=10029 GN=HTR2A PE=2 SV=1)

HSP 1 Score: 63.1586 bits (152), Expect = 2.489e-9
Identity = 49/192 (25.52%), Postives = 96/192 (50.00%), Query Frame = -2

Query:  801 NIIIMVLIRKKRSIEDIKYHYIRSLCISNMILATFNIPVSIIEDFTLLNEQR----TTVCFISVYLDSFLCNQIMLLLLCNTIDCFFRIRNSQLYKLSNRPIPFYLKYFAPWXXXXXXXXXXXXISVQYKCEIINH-VCVLADPNFIIIRSILGFAIPLITSAIFIFITAKVL--------SDIRSKLDLKS 1337
            NI++++ +  ++ +++   +++ SL I++M+L    +PVS++   T+L   R    + +C + +YLD       ++ L   ++D +  I+N   +   N     +LK  A W I +  ++ +    +Q   ++     C+LAD NF++I S + F IPL    I  F+T K L        SD+ ++  L S
Sbjct:   92 NILVIMAVSLEKKLQNATNYFLMSLAIADMLLGFLVMPVSML---TILYGYRWPLPSKLCAVWIYLDVLFSTASIMHLCAISLDRYVAIQNPIHHSRFNSRTKAFLKIIAVWTISVGVSMPIPVFGLQDDSKVFKQGSCLLADDNFVLIGSFVAFFIPLTIMVITYFLTIKSLQKEATLCVSDLSTRAKLAS 280

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BLAST of SMED30023645 vs. UniProt/SwissProt
Match: sp|P14842|5HT2A_RAT (5-hydroxytryptamine receptor 2A OS=Rattus norvegicus OX=10116 GN=Htr2a PE=1 SV=1)

HSP 1 Score: 63.1586 bits (152), Expect = 2.601e-9
Identity = 50/192 (26.04%), Postives = 96/192 (50.00%), Query Frame = -2

Query:  801 NIIIMVLIRKKRSIEDIKYHYIRSLCISNMILATFNIPVSIIEDFTLLNEQR----TTVCFISVYLDSFLCNQIMLLLLCNTIDCFFRIRNSQLYKLSNRPIPFYLKYFAPWXXXXXXXXXXXXISVQYKCEIINH-VCVLADPNFIIIRSILGFAIPLITSAIFIFITAKVL--------SDIRSKLDLKS 1337
            NI++++ +  ++ +++   +++ SL I++M+L    +PVS++   T+L   R    + +C I +YLD       ++ L   ++D +  I+N   +   N     +LK  A W I +  ++ +    +Q   ++     C+LAD NF++I S + F IPL    I  F+T K L        SD+ ++  L S
Sbjct:   92 NILVIMAVSLEKKLQNATNYFLMSLAIADMLLGFLVMPVSML---TILYGYRWPLPSKLCAIWIYLDVLFSTASIMHLCAISLDRYVAIQNPIHHSRFNSRTKAFLKIIAVWTISVGISMPIPVFGLQDDSKVFKEGSCLLADDNFVLIGSFVAFFIPLTIMVITYFLTIKSLQKEATLCVSDLSTRAKLAS 280

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BLAST of SMED30023645 vs. UniProt/SwissProt
Match: sp|P35363|5HT2A_MOUSE (5-hydroxytryptamine receptor 2A OS=Mus musculus OX=10090 GN=Htr2a PE=1 SV=1)

HSP 1 Score: 62.7734 bits (151), Expect = 3.129e-9
Identity = 49/192 (25.52%), Postives = 96/192 (50.00%), Query Frame = -2

Query:  801 NIIIMVLIRKKRSIEDIKYHYIRSLCISNMILATFNIPVSIIEDFTLLNEQR----TTVCFISVYLDSFLCNQIMLLLLCNTIDCFFRIRNSQLYKLSNRPIPFYLKYFAPWXXXXXXXXXXXXISVQYKCEIINH-VCVLADPNFIIIRSILGFAIPLITSAIFIFITAKVL--------SDIRSKLDLKS 1337
            NI++++ +  ++ +++   +++ SL I++M+L    +PVS++   T+L   R    + +C + +YLD       ++ L   ++D +  I+N   +   N     +LK  A W I +  ++ +    +Q   ++     C+LAD NF++I S + F IPL    I  F+T K L        SD+ ++  L S
Sbjct:   92 NILVIMAVSLEKKLQNATNYFLMSLAIADMLLGFLVMPVSML---TILYGYRWPLPSKLCAVWIYLDVLFSTASIMHLCAISLDRYVAIQNPIHHSRFNSRTKAFLKIIAVWTISVGISMPIPVFGLQDDSKVFKEGSCLLADDNFVLIGSFVAFFIPLTIMVITYFLTIKSLQKEATLCVSDLSTRAKLSS 280

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BLAST of SMED30023645 vs. UniProt/SwissProt
Match: sp|O46635|5HT2A_CANLF (5-hydroxytryptamine receptor 2A OS=Canis lupus familiaris OX=9615 GN=HTR2A PE=2 SV=2)

HSP 1 Score: 62.003 bits (149), Expect = 5.389e-9
Identity = 45/173 (26.01%), Postives = 89/173 (51.45%), Query Frame = -2

Query:  834 NIIIMVLIRKKRSIEDIKYHYIRSLCISNMILATFNIPVSIIEDFTLLNEQR----TTVCFISVYLDSFLCNQIMLLLLCNTIDCFFRIRNSQLYKLSNRPIPFYLKYFAPWXXXXXXXXXXXXISVQYKCEIINH-VCVLADPNFIIIRSILGFAIPLITSAIFIFITAKVL 1337
            NI++++ +  ++ +++   +++ SL I++M+L    +PVS++   T+L   R    + +C + +YLD       ++ L   ++D +  I+N   +   N     +LK  A W I +  ++ +    +Q   ++     C+LAD NF++I S + F IPL    I  F+T K L
Sbjct:   92 NILVIMAVSLEKKLQNATNYFLMSLAIADMLLGFLVMPVSML---TILYGYRWPLPSKLCAVWIYLDVLFSTASIMHLCAISLDRYVAIQNPIHHSRFNSRTKAFLKIIAVWTISVGISMPIPVFGLQDDSKVFKEGSCLLADDNFVLIGSFVSFFIPLTIMVITYFLTIKSL 261

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BLAST of SMED30023645 vs. TrEMBL
Match: A0A4E0RHB5 (G_PROTEIN_RECEP_F1_2 domain-containing protein OS=Fasciola hepatica OX=6192 GN=D915_002765 PE=4 SV=1)

HSP 1 Score: 103.219 bits (256), Expect = 1.412e-19
Identity = 64/220 (29.09%), Postives = 110/220 (50.00%), Query Frame = -2

Query:  834 IKSIIYNQSTTITYSN-NKIKFPLDLICAVGIMFCAWILNIIIMVLIRKKRSIEDIKYHYIRSLCISNMILATFNIPVSIIEDFTLLNEQRTTVCFISVYLDSFLCNQIMLLLLCNTIDCFFRIRNSQLYKLS-NRPIPFYLKYFAPWXXXXXXXXXXXXISVQYKCEII------------NHVCVLADPNFIIIRSILGFAIPLITSAIFIFITAKVL 1451
            +K + Y   TT + S    + F       +G++  +WIL  ++ V I + R  + I Y YI S+ ++ +I A+ N P S+I D   L E    +C I ++L++ +C+ ++L LL +++D + R++  Q Y LS  RP    LK  APW          I  ++Q   +++              +C   D NF+I+R+ + FA PL+ S I +F+ A  L
Sbjct:   18 LKRVGYQTRTTQSLSTWGSVTF------VIGMIILSWILTGVVSVAILRNRRTKHISYMYIVSMSVATVIHASLNFPSSVIFDLLDLEEFSDRLCIIWIFLETAICHSVLLHLLGSSLDTYLRLQMPQWYSLSGGRPATVRLKLAAPW----------IVSTLQTTAQVLLGDPLPPARLEEQRLCTCPDVNFLILRTSVAFAFPLLVSVIILFLAAHKL 221

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BLAST of SMED30023645 vs. TrEMBL
Match: A0A5K4F3V3 (Uncharacterized protein OS=Schistosoma mansoni OX=6183 PE=4 SV=1)

HSP 1 Score: 82.8037 bits (203), Expect = 2.262e-13
Identity = 59/189 (31.22%), Postives = 103/189 (54.50%), Query Frame = -2

Query:  843 LDLICAVGIMFCAWILNIIIMVLIRKKRSIEDIKYHYIRSLCISNMILATFNIPVSIIEDFTLLNEQRTTVCFISVYLDSFLCNQIMLLLLCNTIDCFFRIRNSQLY----KLSNRP--IPFYLKYFAPWXXXXXXXXXXXXISVQYKCEII--NHVCVLADPNFIIIRSILGFAIPLITSAIFIFITA 1385
            L +   +  M   WIL+I IM+ + +   ++   Y+YI S+    M+ +  N PV+II D    NE     C IS+  ++ +C+ I+L LL +++D  FR+   + +     +S+R   +   LK  APW++ I+QT   I +S  +    +    +C   D NF+I+R+++ F +PL+TS I +F+TA
Sbjct:   30 LGITVVLLTMIITWILHISIMISVCRNMIMKHTTYYYIISIGFVVMLHSLLNFPVNIISDLIGENEHLQHFCCISIASETIVCHAILLHLLGSSLDTHFRLTKPKWFSNTTNISSRKYLLAIRLKIAAPWIVSILQTGAQIILSDSFPPAYLEEGRLCTSPDINFLILRTLVAFLLPLLTSIIILFMTA 218

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BLAST of SMED30023645 vs. TrEMBL
Match: A0A0X3NHV6 (G_PROTEIN_RECEP_F1_2 domain-containing protein OS=Schistocephalus solidus OX=70667 GN=TR167506 PE=4 SV=1)

HSP 1 Score: 83.1889 bits (204), Expect = 4.267e-13
Identity = 55/208 (26.44%), Postives = 101/208 (48.56%), Query Frame = -2

Query:  834 FPLDLICAVGIMFCAWILNIIIMVLIRKKRS---IEDIKYHYIRSLCISNMILATFNIP---VSIIEDFTLLNEQRTTVCFISVYLDSFLCNQIMLLLLCNTIDCFFRIRNSQLYKLSNRPIP-FYLKYFAPWXXXXXXXXXXXXISVQYKCEIINH---------------VCVLADPNFIIIRSILGFAIPLITSAIFIFITAKVL 1391
             PL L C   ++F  W+ N I++  I +  +   +  + Y+++ S  I+ ++ AT N+P   V+++   T ++      C  S YLD+  CN  +L   C+ +D + R+     Y +S R  P  +LK  +PW+   +Q I  + +S + K +                    CV++DPNF+I+R+ + +A+PLI   +   +  K L
Sbjct:   45 LPLALGCF--LLFATWLANCILLGGILQTLTSPHVVPVSYYFVGSQAIAALLHATLNLPPGLVTLLFGSTPMDSYLRIFCLFSTYLDTLFCNLALLHAFCSGLDSYLRLCGPSTYLVSARRRPTTWLKIGSPWVAAGLQAIGQLALSDRDKAKFFGDCQHPPSTQFADQFEAACVISDPNFLIMRTTIAYALPLIACLVLTGLQMKRL 250

[Close]

BLAST of SMED30023645 vs. TrEMBL
Match: A0A4Z2D5W0 (Octopamine receptor OS=Schistosoma japonicum OX=6182 GN=EWB00_004341 PE=4 SV=1)

HSP 1 Score: 82.4185 bits (202), Expect = 8.362e-13
Identity = 85/308 (27.60%), Postives = 144/308 (46.75%), Query Frame = -2

Query:  495 MFCAWILNIIIMVLIRKKRSIEDIKYHYIRSLCISNMILATFNIPVSIIEDFTLLNEQRTTVCFISVYLDSFLCNQIMLLLLCNTIDCFFRIRN----SQLYKLSNRP--IPFYLKYFAPWXXXXXXXXXXXXISVQYKCEII--NHVCVLADPNFIIIRSILGFAIPLITSAIFIFITAKVLSDIR----------SKLDLKSDKDFENILPEIPLFSVPQYRCTVGTANENIRLRQLDFETVPEETEAKLVQHHCPQHGLLTFPLQEKSGNLFFEPS-SVVYQCSNQPTSSSKHSP-DIVLSSNQD 1358
            M   W++ I I++ I K   ++ I Y YI S+    M+ +  N PV+II D    NE     C IS+  ++ +C+ I+L LL +++D  FR+      S +   SN+   +   LK  APW++ I+QT   I +S  +    +     C   D NF+I+R+++ F +PL+TS I +F+TA  +  ++          S L+++  K    I P+      P+YR      N N +  +   E +P       V H C       F    +S N  F+P     ++  N   + S++S  +  L++N D
Sbjct:   60 MTFTWMIYISIIISICKNIIMKHITYFYIASIGFVVMLHSVLNFPVNIISDLVGENEYIQQFCCISIASETVVCHAILLHLLGSSLDTHFRLTKPKWFSNVTNASNKKHLLAIRLKIAAPWIVSILQTGAQIMLSDPFPPAYLEEGRFCTTPDVNFLILRTLVAFLLPLLTSVIILFMTAHRIQTLQCQKKQHHSKISTLNVQQKKMDNVISPKAS--QQPKYRLITSCINNNGKTYEHKVEVIP----LSFVNHDCYYDCTQKFNFDSRSEN--FQPECETFHENENNRLNKSRYSSIETNLNTNND 359

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BLAST of SMED30023645 vs. TrEMBL
Match: A0A183A614 (Uncharacterized protein OS=Echinostoma caproni OX=27848 GN=ECPE_LOCUS2399 PE=4 SV=1)

HSP 1 Score: 79.7221 bits (195), Expect = 4.906e-12
Identity = 55/209 (26.32%), Postives = 97/209 (46.41%), Query Frame = -2

Query:  819 IMFCAWILNIIIMVLIRKKRS---IEDIKYHYIRSLCISNMILATFNIP---VSIIEDFTLLNEQRTTVCFISVYLDSFLCNQIMLLLLCNTIDCFFRIRNSQLYKLSNRPIP-FYLKYFAPWXXXXXXXXXXXXISVQ---------------------YKCEIINHVCVLADPNFIIIRSILGFAIPLITSAIFIFITAKVLSDIRS 1361
            I+   W+ N I++    +  S   I  + Y ++ S  I+ ++  T N+P   V +I   T +  +    C  S YLD+  CN   L    +++D + R+RN   Y  S R  P  +LK  +PWL+  +Q I  + +S +                     +  + +   C+L DPNF+IIR+ + +A+PLI   I + +  + L  +R+
Sbjct:   17 ILLATWLANCILLGGALQTASSGRIVPVAYFFVGSQAIAALLYVTLNLPPAAVGLIFGSTHVQSRIHVFCLYSTYLDTLFCNLTFLQTFFSSLDAYLRLRNPTFYLTSARRRPALWLKVGSPWLVAGLQAIGQLALSDRDQVSLHFASIKSADDQEDGHSWGVQSLQTTCLLPDPNFLIIRTAIAYALPLIVCIILVGLQLRCLRGLRN 225

[Close]

BLAST of SMED30023645 vs. Ensembl Cavefish
Match: drd2b (D(2) dopamine receptor A [Source:NCBI gene;Acc:103035621])

HSP 1 Score: 64.3142 bits (155), Expect = 1.242e-10
Identity = 87/433 (20.09%), Postives = 182/433 (42.03%), Query Frame = -2

Query:   99 NIIIMVLIRKKRSIEDIKYHYIRSLCISNMILATFNIPVSIIEDFTLLNEQRTTV--CFISVYLDSFLCNQIMLLLLCNTIDCFFRIRNSQLYKL---SNRPIPFYLKYFAPWXXXXXXXXXXXXISVQYKCEII----------NHVCVLADPNFIIIRSILGFAIPLITSAIFIFITAKVLSDIRSKLDLKSDKDFENILPEIPLFSVPQYRCTVGTANENIRLRQLDFET---VPEETEAKLVQHHCPQHGLLTFPLQEKSGNLFFEPSSVVYQCSNQPTSSSKHSPDIVLSSNQDFTKPISMLHQDQPKESQTP--SAAINQEILQCADKEFTINKQNFNKRIEEFRQERKXXXXXXXXXXXXIMFWGPYITSTLIQGICILPNCPLSISIPKINEFKWLSYSSSVIIPLFILVMDNRVRRTFCYILRC 1337
            N+++ + + ++++++    + I SL ++++++AT  +P  +  +  ++ E R +   C I V LD  +C   +L L   +ID +  +    LY     S R +   +     W++           S    C ++          + VC +A+P F++  SI+ F +P I + +       VL   R +++ K +    +  P+ PL    + +C   T  E+++L  +  +T   +P + +  +      + GL+T     +   L   P+  +       T S  H+  +      +  +P         K++QTP  + +   + L       T+ K   +KR     +E+K + +   +  + I+ W P+  + +++  C     PL +     N F WL Y +S + P+     +   R+ F  IL C
Sbjct:   44 NVLVCMAVSREKALQTTTNYLIVSLAVADLLVATLVMPWVVYLE--VVGEWRFSKIHCDIFVTLDVMMCTASILNLCAISIDRYTAVAMPMLYNTRYSSKRRVTVMISVV--WVL-----------SFAISCPLLFGLNNTATRDDSVCEIANPAFVVYSSIMSFYVPFIITLLVYVQIYVVLRKRRKRVNTKRNCQKADAEPQPPL----KEKC---THPEDVKLCTVIVKTNGSLPVKNKKVVQPGTGVEMGLMTSTSPPEKMKLAAPPAVNLLA-----TQSPSHASPL-----HEEWQPHDDEKNGHAKDAQTPKETKSFETQALPNGKTRTTVTK-TMSKRKMSQHKEKKATQMLAIVLGVFIICWLPFFITHILKTHCTSCYVPLEM----YNAFTWLGYVNSAVNPIIYTTFNVEFRKAFMKILHC 439

[Close]

BLAST of SMED30023645 vs. Ensembl Cavefish
Match: htr2ab (5-hydroxytryptamine (serotonin) receptor 2A, genome duplicate b [Source:ZFIN;Acc:ZDB-GENE-120215-60])

HSP 1 Score: 58.9214 bits (141), Expect = 6.842e-9
Identity = 45/173 (26.01%), Postives = 87/173 (50.29%), Query Frame = -2

Query:  834 NIIIMVLIRKKRSIEDIKYHYIRSLCISNMILATFNIPVSIIEDFTLLNEQRT----TVCFISVYLDSFLCNQIMLLLLCNTIDCFFRIRNSQLYKLSNRPIPFYLKYFAPWXXXXXXXXXXXXISVQYKCEII-NHVCVLADPNFIIIRSILGFAIPLITSAIFIFITAKVL 1337
            NI++++ +  +R +++   +++ SL I++M+L    +PVS++   T+L   R      +C I +YLD       ++ L   ++D +  IRN   +  SN      +K  A W I +  ++ +  I +    ++  N  C L + NF++I S + F +PLI   +  F+T   L
Sbjct:   98 NILVIMAVSLERKLQNATNYFLMSLAITDMLLGLLVMPVSMV---TILYNYRWPFPFVLCPIWIYLDVLFSTASIMHLCAISLDRYIAIRNPIQHSRSNSRARARVKIAAAWTISVGISMPVPVIGLYDHSKVFHNGSCQLKEDNFVLIGSFVAFFVPLIIMVVTYFLTISAL 267

[Close]

BLAST of SMED30023645 vs. Ensembl Cavefish
Match: htr2cl2 (5-hydroxytryptamine (serotonin) receptor 2C, G protein-coupled-like 2 [Source:ZFIN;Acc:ZDB-GENE-120215-109])

HSP 1 Score: 54.299 bits (129), Expect = 1.196e-7
Identity = 37/191 (19.37%), Postives = 87/191 (45.55%), Query Frame = -2

Query:  765 NIIIMVLIRKKRSIEDIKYHYIRSLCISNMILATFNIPVSIIEDFTLLNEQRTTVCFISVYLDSFLCNQIMLLLLCNTIDCFFRIRNSQLYKLSNRPIPFYLKYFAPWXXXXXXXXXXXXISVQYKCEIINHVCVLADPNFIIIRSILGFAIPLITSAIFIFITAKVLSDIRSKLDLKSDKDFENILPEIP 1337
            NI++++ +  ++ +++    ++RSL +++M++    +P S++            +C + ++LD       ++ L   ++D +  IRN   +  SN       K    W I ++     +      +   +N  C L +P+F+++ S + F +PL+   +   +T KVL    +    +    + N+ P+ P
Sbjct:   17 NILVILAVSLEKKLQNATNFFLRSLAVADMLVGVLVMPASLLNILNYSWPLPQVLCPMWIFLDVLFSTASIMHLCAISLDRYIGIRNPIQHSRSNSSCRAMAKITVVWTISMVSLPIPVIGLQDEQKVFVNGTCQLNEPHFVLVGSFVAFFVPLLIMVVCYCLTVKVLQQHTAAFS-QDRGSYVNVNPKQP 206

[Close]

BLAST of SMED30023645 vs. Ensembl Cavefish
Match: htr2cl1 (5-hydroxytryptamine receptor 2C-like [Source:NCBI gene;Acc:103044140])

HSP 1 Score: 52.7582 bits (125), Expect = 5.462e-7
Identity = 40/172 (23.26%), Postives = 86/172 (50.00%), Query Frame = -2

Query:  834 NIIIMVLIRKKRSIEDIKYHYIRSLCISNMILATFNIPVSIIE---DFTLLNEQRTTVCFISVYLDSFLCNQIMLLLLCNTIDCFFRIRNSQLYKLSNRPIPFYLKYFAPWXXXXXXXXXXXXISVQYKCEI-INHVCVLADPNFIIIRSILGFAIPLITSAIFIFITAKVL 1337
            NI++++ +  ++ +++    ++RSL +++M++    +P+S+I    D+         +C I +YLD       ++ L   ++D +  I N   +   N      LK  A W I I  ++ +  I +  + ++ +NH CVL +  F+++ S + F IPL+   +   +T + L
Sbjct:   84 NILVILAVSLEKKLQNATNFFLRSLAVADMLVGILVMPISLINILYDYAW--PLPNALCPIWIYLDVLFSTASIMHLCAISLDRYVAICNPIQHSRFNSRTKAMLKIAAVWTISIGISMPIPVIGLSNEDKVFVNHSCVLNEERFVLVGSFVAFFIPLVIMVVTYCLTVQAL 253

[Close]

BLAST of SMED30023645 vs. Ensembl Medaka
Match: htr2aa (5-hydroxytryptamine receptor 2A [Source:NCBI gene;Acc:101173822])

HSP 1 Score: 56.225 bits (134), Expect = 4.180e-8
Identity = 39/175 (22.29%), Postives = 87/175 (49.71%), Query Frame = -2

Query:  828 NIIIMVLIRKKRSIEDIKYHYIRSLCISNMILATFNIPVSIIEDFTLLNEQRT----TVCFISVYLDSFLCNQIMLLLLCNTIDCFFRIRNSQLYKLSNRPIPFYLKYFAPWXXXXXXXXXXXXISVQYKCEII-NHVCVLADPNFIIIRSILGFAIPLITSAIFIFITAKVLSD 1337
            NI++++ +  ++ +++   +++ SL +++M+L    +PVS++   T+L + R      +C I +YLD       ++ L   ++D +  IRN   +   N      +K  A W I    ++ +  + ++   ++     C+L D NF+++ S + F +PL    +  F+T   L +
Sbjct:  105 NILVILAVSLEKKLQNATNYFLMSLAVADMLLGILVMPVSMV---TILYDYRWPLPPDLCPIWIYLDVLFSTASIMHLCAISLDRYIAIRNPIHHSRFNSHTKARIKIMAVWTISAGISMPIPVLGLRDHSKVFKGDSCLLTDNNFVLVGSFVAFFVPLTIMVVTYFLTINALQN 276

[Close]

BLAST of SMED30023645 vs. Ensembl Medaka
Match: htr2cl1 (5-hydroxytryptamine receptor 2C-like [Source:NCBI gene;Acc:101165845])

HSP 1 Score: 51.9878 bits (123), Expect = 1.043e-6
Identity = 40/172 (23.26%), Postives = 88/172 (51.16%), Query Frame = -2

Query:  834 NIIIMVLIRKKRSIEDIKYHYIRSLCISNMILATFNIPVSIIE---DFTLLNEQRTTVCFISVYLDSFLCNQIMLLLLCNTIDCFFRIRNSQLYKLSNRPIPFYLKYFAPWXXXXXXXXXXXXISVQYKCEI-INHVCVLADPNFIIIRSILGFAIPLITSAIFIFITAKVL 1337
            NI++++ +  ++ +++    ++RSL +++M++    +P+S+I    D+       + +C I +YLD       ++ L   ++D +  IRN   +   N      +K  A W I I  ++ +  I +  + ++ ++  CVL +  F++I S + F IPL+   +   +T +VL
Sbjct:   92 NILVILAVSLEKKLQNATNFFLRSLAVADMLVGILVMPISLINILYDYAW--PLPSALCPIWIYLDVLFSTASIMHLCAISLDRYVAIRNPIEHSRFNSRTKAMMKIAAVWTISIGVSMPIPVIGLLNEDKVFVDGSCVLNEERFMLIGSFVAFFIPLVIMVVTYCLTIQVL 261

[Close]

Query:  834 NIIIMVLIRKKRSIEDIKYHYIRSLCISNMILATFNIPVSIIE---DFTLLNEQRTTVCFISVYLDSFLCNQIMLLLLCNTIDCFFRIRNSQLYKLSNRPIPFYLKYFAPWXXXXXXXXXXXXISVQYKCEI-INHVCVLADPNFIIIRSILGFAIPLITSAIFIFITAKVL 1337
            NI++++ +  ++ +++    ++RSL +++M++    +P+S+I    D+       + +C I +YLD       ++ L   ++D +  IRN   +   N      +K  A W I I  ++ +  I +  + ++ ++  CVL +  F++I S + F IPL+   +   +T +VL
Sbjct:   92 NILVILAVSLEKKLQNATNFFLRSLAVADMLVGILVMPISLINILYDYAW--PLPSALCPIWIYLDVLFSTASIMHLCAISLDRYVAIRNPIEHSRFNSRTKAMMKIAAVWTISIGVSMPIPVIGLLNEDKVFVDGSCVLNEERFMLIGSFVAFFIPLVIMVVTYCLTIQVL 261

[Close]

BLAST of SMED30023645 vs. Planmine SMEST
Match: SMESG000077254.1 (SMESG000077254.1)

HSP 1 Score: 863.988 bits (2231), Expect = 0.000e+0
Identity = 455/457 (99.56%), Postives = 457/457 (100.00%), Query Frame = -2

Query:   84 MIKSIIYNQSTTITYSNNKIKFPLDLICAVGIMFCAWILNIIIMVLIRKKRSIEDIKYHYIRSLCISNMILATFNIPVSIIEDFTLLNEQRTTVCFISVYLDSFLCNQIMLLLLCNTIDCFFRIRNSQLYKLSNRPIPFYLKYFAPWXXXXXXXXXXXXISVQYKCEIINHVCVLADPNFIIIRSILGFAIPLITSAIFIFITAKVLSDIRSKLDLKSDKDFENILPEIPLFSVPQYRCTVGTANENIRLRQLDFETVPEETEAKLVQHHCPQHGLLTFPLQEKSGNLFFEPSSVVYQCSNQPTSSSKHSPDIVLSSNQDFTKPISMLHQDQPKESQTPSAAINQEILQCADKEFTINKQNFNKRIEEFRQERKXXXXXXXXXXXXIMFWGPYITSTLIQGICILPNCPLSISIPKINEFKWLSYSSSVIIPLFILVMDNRVRRTFCYILRCSYDES 1454
            MIKSIIYNQSTTITYSNNKIKFPLDLICA+GIMFCAWILNIIIMVLIRKKR+IEDIKYHYIRSLCISNMILATFNIPVSIIEDFTLLNEQRTTVCFISVYLDSFLCNQIMLLLLCNTIDCFFRIRNSQLYKLSNRPIPFYLKYFAPWLICIIQTITLITISVQYKCEIINHVCVLADPNFIIIRSILGFAIPLITSAIFIFITAKVLSDIRSKLDLKSDKDFENILPEIPLFSVPQYRCTVGTANENIRLRQLDFETVPEETEAKLVQHHCPQHGLLTFPLQEKSGNLFFEPSSVVYQCSNQPTSSSKHSPDIVLSSNQDFTKPISMLHQDQPKESQTPSAAINQEILQCADKEFTINKQNFNKRIEEFRQERKNSIINLSLCIILIMFWGPYITSTLIQGICILPNCPLSISIPKINEFKWLSYSSSVIIPLFILVMDNRVRRTFCYILRCSYDES
Sbjct:    1 MIKSIIYNQSTTITYSNNKIKFPLDLICAIGIMFCAWILNIIIMVLIRKKRTIEDIKYHYIRSLCISNMILATFNIPVSIIEDFTLLNEQRTTVCFISVYLDSFLCNQIMLLLLCNTIDCFFRIRNSQLYKLSNRPIPFYLKYFAPWLICIIQTITLITISVQYKCEIINHVCVLADPNFIIIRSILGFAIPLITSAIFIFITAKVLSDIRSKLDLKSDKDFENILPEIPLFSVPQYRCTVGTANENIRLRQLDFETVPEETEAKLVQHHCPQHGLLTFPLQEKSGNLFFEPSSVVYQCSNQPTSSSKHSPDIVLSSNQDFTKPISMLHQDQPKESQTPSAAINQEILQCADKEFTINKQNFNKRIEEFRQERKNSIINLSLCIILIMFWGPYITSTLIQGICILPNCPLSISIPKINEFKWLSYSSSVIIPLFILVMDNRVRRTFCYILRCSYDES 457

[Close]

BLAST of SMED30023645 vs. Planmine SMEST
Match: SMESG000031149.1 (SMESG000031149.1)

HSP 1 Score: 184.882 bits (468), Expect = 4.026e-52
Identity = 157/515 (30.49%), Postives = 246/515 (47.77%), Query Frame = -2

Query:   99 YSNNKIKFPLDLICAVGIMFCAWILNIIIMVLIRKKRSIEDIKYHYIRSLCISNMILATFNIPVSIIEDFTLLNEQRTTVCFISVYLDSFLCNQIMLLLLCNTIDCFFRIRNSQLYKLSNRPIPFYLKYFAPWXXXXXXXXXXXXISVQYKCEIINHVCVLADPNFIIIRSILGFAIPLITSAIFIFITAKVLSDIRSKLDL-KSDKDFENIL-PEIPLFSV--PQYRCTVGTANENIRLRQLD-------FETVPEETEAKLVQHHCPQHGLLTFPLQEKSGNLF-------------FEPS-SVVYQCSN------QPT--------------SSSKHSPDIVLSSNQDFTKPIS------------MLHQDQPKE----------SQTPSAAI-------NQEILQCADKEFTINK--QNFNKRIEEFR-QERKXXXXXXXXXXXXIMFWGPYITSTLIQGICILPNCPLSISIPKINEFKWLSYSSSVIIPLFILVMDNRVRRTFCYILRC 1412
            Y  +   FP++L+ A  I+  AWILN+  +  +R   S   + Y ++ S+C++N+ LAT + P  II    +    +  +C    YLD+ LCN ++L LL  ++D + R+R+   Y  S + I   LK  APW +  +Q+   + I+   +  I + VC ++DPNF+I+RS++ F  P+  S+I +F+ AK LS    KL   KS +  E+I+  E   F +  P++        +   LR  D        +T           H CPQHG+L+ P+ ++S ++F               PS  ++YQC +      +P+              S +KH PD++ +     + PIS             +HQ +             +  PS ++       NQ I +    +  I K   +  K +   R  ER    INLSLCII I+FWGP+I  +LIQG+C  P C L IS  +INEFKWLSY   +I PL IL++D  +R+     L C
Sbjct:    5 YVGDNNNFPVNLLLAGVIIVIAWILNLSAICAMRSLNSGTRVVYAFLSSMCVANLFLATIDFPPPIISKILVSTSMQHIMCVSWTYLDTLLCNVLILHLLGCSLDSYLRLRHPSRYMDSTKQILTCLKLSAPWTLSFVQSTAFMAIAQISESTIQDGVCFISDPNFLILRSVMCFGFPISVSSILVFLAAKSLSSFDRKLQTYKSKEKKEDIMTTEAETFQLLSPRHNLINRDETQETILRTSDDPNTVVSSQTFDCTNTLNYYHHQCPQHGILSIPI-DRSAHIFPNSSHVSKYDPFIMSPSCDILYQCCSTQAAIKEPSVLSENVYTSNYIYPSDTKHCPDVIPTMQSSTSDPISNPLVNNSILRNPNMHQSRSSGDILDSHPHYCTLPPSQSLDFQRIYENQIIPETNVTKSHIEKPSASITKPLNLVRSHERTTIAINLSLCIISIIFWGPFIIGSLIQGVCKPPFCILPISNSRINEFKWLSYVLCIIYPLVILIIDRDIRKVIRSSLNC 518

[Close]

BLAST of SMED30023645 vs. Planmine SMEST
Match: SMESG000031149.1 (SMESG000031149.1)

HSP 1 Score: 184.496 bits (467), Expect = 4.392e-52
Identity = 156/510 (30.59%), Postives = 246/510 (48.24%), Query Frame = -2

Query:   99 IKFPLDLICAVGIMFCAWILNIIIMVLIRKKRSIEDIKYHYIRSLCISNMILATFNIPVSIIEDFTLLNEQRTTVCFISVYLDSFLCNQIMLLLLCNTIDCFFRIRNSQLYKLSNRPIPFYLKYFAPWXXXXXXXXXXXXISVQYKCEIINHVCVLADPNFIIIRSILGFAIPLITSAIFIFITAKVLSDIRSKLDL-KSDKDFENIL-PEIPLFSV--PQYRCTVGTANENIRLRQLD-------FETVPEETEAKLVQHHCPQHGLLTFPLQEKSGNLF-------------FEPS-SVVYQCSN------QPT--------------SSSKHSPDIVLSSNQDFTKPIS------------MLHQDQPKE----------SQTPSAAI-------NQEILQCADKEFTINK--QNFNKRIEEFR-QERKXXXXXXXXXXXXIMFWGPYITSTLIQGICILPNCPLSISIPKINEFKWLSYSSSVIIPLFILVMDNRVRRTFCYILRC 1397
            ++FP++L+ A  I+  AWILN+  +  +R   S   + Y ++ S+C++N+ LAT + P  II    +    +  +C    YLD+ LCN ++L LL  ++D + R+R+   Y  S + I   LK  APW +  +Q+   + I+   +  I + VC ++DPNF+I+RS++ F  P+  S+I +F+ AK LS    KL   KS +  E+I+  E   F +  P++        +   LR  D        +T           H CPQHG+L+ P+ ++S ++F               PS  ++YQC +      +P+              S +KH PD++ +     + PIS             +HQ +             +  PS ++       NQ I +    +  I K   +  K +   R  ER    INLSLCII I+FWGP+I  +LIQG+C  P C L IS  +INEFKWLSY   +I PL IL++D  +R+     L C
Sbjct:    1 MQFPVNLLLAGVIIVIAWILNLSAICAMRSLNSGTRVVYAFLSSMCVANLFLATIDFPPPIISKILVSTSMQHIMCVSWTYLDTLLCNVLILHLLGCSLDSYLRLRHPSRYMDSTKQILTCLKLSAPWTLSFVQSTAFMAIAQISESTIQDGVCFISDPNFLILRSVMCFGFPISVSSILVFLAAKSLSSFDRKLQTYKSKEKKEDIMTTEAETFQLLSPRHNLINRDETQETILRTSDDPNTVVSSQTFDCTNTLNYYHHQCPQHGILSIPI-DRSAHIFPNSSHVSKYDPFIMSPSCDILYQCCSTQAAIKEPSVLSENVYTSNYIYPSDTKHCPDVIPTMQSSTSDPISNPLVNNSILRNPNMHQSRSSGDILDSHPHYCTLPPSQSLDFQRIYENQIIPETNVTKSHIEKPSASITKPLNLVRSHERTTIAINLSLCIISIIFWGPFIIGSLIQGVCKPPFCILPISNSRINEFKWLSYVLCIIYPLVILIIDRDIRKVIRSSLNC 509

[Close]

BLAST of SMED30023645 vs. Planmine SMEST
Match: SMESG000063199.1 (SMESG000063199.1)

HSP 1 Score: 110.153 bits (274), Expect = 3.414e-26
Identity = 99/389 (25.45%), Postives = 184/389 (47.30%), Query Frame = -2

Query:   93 LDSFLCNQIMLLLLCNTIDCFFRIRNSQLYKLSNRPIPFYLKYFAPWXXXXXXXXXXXXISVQYKCEIINHVCVLADPNFIIIRSILGFAIPLIT------------------SAIFIFITAKVLSDIRSKLDLKSDK-----DFENILPEIPLFSVPQYRCTVGTANENIRLRQLDFE----TVPEETEAKLVQHHCPQHGLLTFPL--QEKSGNLFFEPSSVVYQCSNQPTSSSKHS------PDIVLSSNQDFTKPISMLHQDQPKESQTPSAAINQEILQCADKEFTINKQNFNKRIEEFRQERKXXXXXXXXXXXXIMFWGPYITSTLIQGICILPNCPLSISIPKINEFKWLSYSSSVIIPLFILVMDNRVRRTFCYILRCSY 1154
            +++FLCN   L +LC+++D FFR++N +LY  S RP   +LK  APW++  IQ    +TI+ Q        +C++ D NF+I+RS++ +A+PL+                   S + +    +  S  +     +SD+     DFEN      +    +        + +I + QL+       +     +++++H+CP+HGL+TFP+  QE    +    +S +   +++ + S+ H       PDI   +    T P    ++D  K                    +  NK+++   +  ++ E+ +  IN+ +CII I  W P I STL   +C    C + IS+ +IN+F+W +++  +  P   L +D+ +R++    L CS+
Sbjct:    1 MNTFLCNLAFLHVLCSSLDTFFRLKNPRLYLDSQRPQTTFLKVSAPWIVTTIQAACQMTINNQKNISTKYSLCLINDGNFLILRSVISYAVPLVVCVILIILQIIYLRFLNSVSVVNLDFLLRSYSINKVNDKCESDETCQLNDFENSQSNNEINEDEEMISPTNPVDNSISMEQLNQSLQSPKLLSSNPSRILKHNCPKHGLITFPIVIQENPQIIDLTSTSNLLMMNSERSKSNLHKSVSFEMPDITNMAKSSETIPDPRFNKDSNK--------------------YLTNKKSW---LRMYKTEQLSIAINMIMCIISIGVWSPLILSTLAYKLCEESPCTIYISVDRINDFEWWAFAGGLAYPCIFLFVDSTIRKSLKSYL-CSH 365

[Close]

BLAST of SMED30023645 vs. Planmine SMEST
Match: SMESG000039485.1 (SMESG000039485.1)

HSP 1 Score: 68.1662 bits (165), Expect = 5.392e-12
Identity = 43/145 (29.66%), Postives = 79/145 (54.48%), Query Frame = -2

Query:  858 KYHYIRSLCISNMILATFNIPVSIIEDFTLLNEQ--RTTVCFISVYLDSFLCNQIMLLLLCNTIDCFFRIRNSQLYKLSNRPIPFYLKYFAPWXXXXXXXXXXXXISVQYKCEIINHVCVLADPNFIIIRSILGFAIPLITSAIF 1286
             Y++I S+ ++N++  +FN P+ I+  +   N        C I + +++  CN   L +L +++D F R+ + ++Y  S RP    LK  APW++  IQ    ++IS +         C++ D NF+I+RS++ FA+PL+   I 
Sbjct:   69 NYYFIVSISVANILAVSFNPPIIIVFRWLNFNNNPLWKVYCLIEISINTIFCNLAFLHILFSSLDTFLRLYSPKMYIYSQRPSTNVLKISAPWVVTCIQAGCQLSISSKDSLRSRLLHCLIDDVNFLILRSVISFAVPLLACVIL 213

The following BLAST results are available for this feature:

BLAST of SMED30023645 vs. Ensembl Human
BLAST of SMED30023645 vs. Ensembl Celegans
BLAST of SMED30023645 vs. Ensembl Fly
BLAST of SMED30023645 vs. Ensembl Zebrafish
BLAST of SMED30023645 vs. Ensembl Xenopus
BLAST of SMED30023645 vs. Ensembl Mouse
BLAST of SMED30023645 vs. UniProt/SwissProt
BLAST of SMED30023645 vs. TrEMBL
BLAST of SMED30023645 vs. Ensembl Cavefish
BLAST of SMED30023645 vs. Ensembl Sea Lamprey
BLAST of SMED30023645 vs. Ensembl Yeast
BLAST of SMED30023645 vs. Ensembl Nematostella
BLAST of SMED30023645 vs. Ensembl Medaka
BLAST of SMED30023645 vs. Planmine SMEST

BLAST of SMED30023645 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 2


Match Name	E-value	Identity	Description
HTR2A	1.144e-9	25.52	5-hydroxytryptamine receptor 2A [Source:HGNC Symbo...	[more]
HTR2A	1.144e-9	25.52	5-hydroxytryptamine receptor 2A [Source:HGNC Symbo...	[more]

BLAST of SMED30023645 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 2


Match Name	E-value	Identity	Description
ser-1	3.587e-6	22.36	SERotonin/octopamine receptor family; Serotonin re...	[more]
ser-1	3.657e-6	22.22	SERotonin/octopamine receptor family; Serotonin re...	[more]

BLAST of SMED30023645 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30023645 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
htr2ab	2.856e-10	26.01	5-hydroxytryptamine (serotonin) receptor 2A, genom...	[more]
drd2b	7.967e-10	20.96	dopamine receptor D2b [Source:ZFIN;Acc:ZDB-GENE-03...	[more]
drd2b	7.967e-10	20.96	dopamine receptor D2b [Source:ZFIN;Acc:ZDB-GENE-03...	[more]
drd2b	7.967e-10	20.96	dopamine receptor D2b [Source:ZFIN;Acc:ZDB-GENE-03...	[more]
htr2cl1	6.278e-7	23.26	5-hydroxytryptamine (serotonin) receptor 2C, G pro...	[more]

BLAST of SMED30023645 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5


Match Name	E-value	Identity	Description
slc25a29	5.056e-9	26.59	solute carrier family 25 (mitochondrial carnitine/...	[more]
slc25a29	5.170e-9	26.59	solute carrier family 25 (mitochondrial carnitine/...	[more]
slc25a29	5.277e-9	26.59	solute carrier family 25 (mitochondrial carnitine/...	[more]
slc25a29	6.774e-9	26.59	solute carrier family 25 (mitochondrial carnitine/...	[more]
DRD2	3.870e-8	19.79	dopamine receptor D2 [Source:NCBI gene;Acc:1004930...	[more]

BLAST of SMED30023645 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 3


Match Name	E-value	Identity	Description
Htr2a	4.474e-10	25.52	5-hydroxytryptamine (serotonin) receptor 2A [Sourc...	[more]
Htr2c	3.265e-6	23.98	5-hydroxytryptamine (serotonin) receptor 2C [Sourc...	[more]
Htr2c	3.265e-6	23.98	5-hydroxytryptamine (serotonin) receptor 2C [Sourc...	[more]

BLAST of SMED30023645 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5


Match Name	E-value	Identity	Description
sp\|P50129\|5HT2A_PIG	2.235e-9	25.52	5-hydroxytryptamine receptor 2A OS=Sus scrofa OX=9...	[more]
sp\|P18599\|5HT2A_CRIGR	2.489e-9	25.52	5-hydroxytryptamine receptor 2A OS=Cricetulus gris...	[more]
sp\|P14842\|5HT2A_RAT	2.601e-9	26.04	5-hydroxytryptamine receptor 2A OS=Rattus norvegic...	[more]
sp\|P35363\|5HT2A_MOUSE	3.129e-9	25.52	5-hydroxytryptamine receptor 2A OS=Mus musculus OX...	[more]
sp\|O46635\|5HT2A_CANLF	5.389e-9	26.01	5-hydroxytryptamine receptor 2A OS=Canis lupus fam...	[more]

BLAST of SMED30023645 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A4E0RHB5	1.412e-19	29.09	G_PROTEIN_RECEP_F1_2 domain-containing protein OS=...	[more]
A0A5K4F3V3	2.262e-13	31.22	Uncharacterized protein OS=Schistosoma mansoni OX=...	[more]
A0A0X3NHV6	4.267e-13	26.44	G_PROTEIN_RECEP_F1_2 domain-containing protein OS=...	[more]
A0A4Z2D5W0	8.362e-13	27.60	Octopamine receptor OS=Schistosoma japonicum OX=61...	[more]
A0A183A614	4.906e-12	26.32	Uncharacterized protein OS=Echinostoma caproni OX=...	[more]

BLAST of SMED30023645 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 4


Match Name	E-value	Identity	Description
drd2b	1.242e-10	20.09	D(2) dopamine receptor A [Source:NCBI gene;Acc:103...	[more]
htr2ab	6.842e-9	26.01	5-hydroxytryptamine (serotonin) receptor 2A, genom...	[more]
htr2cl2	1.196e-7	19.37	5-hydroxytryptamine (serotonin) receptor 2C, G pro...	[more]
htr2cl1	5.462e-7	23.26	5-hydroxytryptamine receptor 2C-like [Source:NCBI ...	[more]

BLAST of SMED30023645 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30023645 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30023645 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30023645 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 3


Match Name	E-value	Identity	Description
htr2aa	4.180e-8	22.29	5-hydroxytryptamine receptor 2A [Source:NCBI gene;...	[more]
htr2cl1	1.043e-6	23.26	5-hydroxytryptamine receptor 2C-like [Source:NCBI ...	[more]
htr2cl1	1.043e-6	23.26	5-hydroxytryptamine receptor 2C-like [Source:NCBI ...	[more]

BLAST of SMED30023645 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5


Match Name	E-value	Identity	Description
SMESG000077254.1	0.000e+0	99.56	SMESG000077254.1	[more]
SMESG000031149.1	4.026e-52	30.49	SMESG000031149.1	[more]
SMESG000031149.1	4.392e-52	30.59	SMESG000031149.1	[more]
SMESG000063199.1	3.414e-26	25.45	SMESG000063199.1	[more]
SMESG000039485.1	5.392e-12	29.66	SMESG000039485.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence
protein sequence

transcript sequence

>SMED30023645 ID=SMED30023645|Name=SMED30023645|organism=Schmidtea mediterranea sexual|type=transcript|length=1479bp
TTTTTTTCAAAGTTGAAGAATTTATTTTCAAATAATAATAAAAGTATAAA
ATATAAATACATATAAATAAAAAATATCTCTCACGATTCATCATATGAAC
ATCTAAGAATGTAACAAAATGTTCGCCTTACTCGATTATCCATGACAAGA
ATGAAGAGTGGAATAATTACAGAAGAACTATAAGAAAGCCACTTAAATTC
ATTTATCTTTGGAATAGAAATGCTAAGAGGACAGTTTGGTAAAATGCAAA
TCCCTTGGATTAATGTTGAAGTAATGTAGGGACCCCAGAACATTATAAGA
ATTATACAGAGAGACAAATTTATTATGCTGTTTTTTCGTTCTTGTCGGAA
TTCTTCAATTCGTTTATTGAAATTTTGTTTGTTTATAGTGAATTCTTTAT
CGGCGCATTGTAATATTTCTTGATTAATTGCAGCCGAGGGAGTTTGGGAT
TCTTTTGGTTGATCTTGGTGAAGCATTGAAATTGGTTTTGTAAAATCTTG
ATTTGAGGACAAAACAATATCCGGTGAATGTTTGCTGCTGGAGGTTGGCT
GATTTGAGCATTGATAAACAACACTGCTTGGTTCGAAAAAGAGATTTCCA
GATTTTTCTTGCAACGGAAAAGTCAGTAATCCGTGCTGAGGACAATGGTG
TTGAACTAATTTGGCTTCGGTTTCTTCTGGTACAGTTTCGAAATCCAATT
GCCTCAGTCGGATATTTTCATTAGCTGTACCGACAGTACAACGATATTGT
GGAACAGAAAACAAAGGAATTTCTGGTAGAATGTTTTCAAAATCTTTGTC
AGATTTCAAATCCAATTTTGACCGAATGTCACTTAATACCTTTGCTGTAA
TAAAAATAAAAATAGCACTTGTAATTAATGGAATGGCGAATCCAAGAATG
GAGCGAATTATGATAAAATTTGGATCCGCTAACACGCAAACGTGGTTGAT
AATTTCACATTTATATTGAACTGAAATAGTGATGAGAGTGATTGTTTGAA
TGATACAAATGAGCCAGGGCGCAAAATATTTCAGATAAAATGGAATGGGC
CTGTTCGATAGTTTGTAGAGTTGACTGTTTCTTATTCGAAAAAAACAGTC
TATTGTATTACAGAGCAGTAAAAGCATTATTTGGTTACAAAGGAAAGAAT
CCAAGTATACAGAAATAAAACATACTGTCGTTCTTTGCTCATTCAGTAAT
GTAAAATCTTCAATTATTGAAACGGGAATATTGAATGTTGCCAAAATCAT
ATTGGAAATGCACAGTGACCGGATGTAGTGATATTTTATATCCTCAATTG
ATCGTTTTTTCCTTATTAATACCATAATAATAATATTTAGAATCCAGGCG
CAAAACATGATTCCAACAGCACAAATTAAATCAAGAGGAAATTTGATTTT
ATTGTTTGAATAGGTGATAGTAGTGGATTGATTATAAATAATTGATTTTA
TCATATTTTACTATTTATTATAAAAAAAA

protein sequence of SMED30023645-orf-1

>SMED30023645-orf-1 ID=SMED30023645-orf-1|Name=SMED30023645-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=458bp
MIKSIIYNQSTTITYSNNKIKFPLDLICAVGIMFCAWILNIIIMVLIRKK
RSIEDIKYHYIRSLCISNMILATFNIPVSIIEDFTLLNEQRTTVCFISVY
LDSFLCNQIMLLLLCNTIDCFFRIRNSQLYKLSNRPIPFYLKYFAPWLIC
IIQTITLITISVQYKCEIINHVCVLADPNFIIIRSILGFAIPLITSAIFI
FITAKVLSDIRSKLDLKSDKDFENILPEIPLFSVPQYRCTVGTANENIRL
RQLDFETVPEETEAKLVQHHCPQHGLLTFPLQEKSGNLFFEPSSVVYQCS
NQPTSSSKHSPDIVLSSNQDFTKPISMLHQDQPKESQTPSAAINQEILQC
ADKEFTINKQNFNKRIEEFRQERKNSIINLSLCIILIMFWGPYITSTLIQ
GICILPNCPLSISIPKINEFKWLSYSSSVIIPLFILVMDNRVRRTFCYIL
RCSYDES*

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0000418	head
PLANA:0003104	enteric muscle cell

Vocabulary: **INTERPRO**
Term	Definition
IPR000276	GPCR_Rhodpsn
IPR017452	GPCR_Rhodpsn_7TM

Vocabulary: **molecular function**
Term	Definition
GO:0004930	G-protein coupled receptor activity

Vocabulary: **cellular component**
Term	Definition
GO:0016021	integral component of membrane

Vocabulary: **biological process**
Term	Definition
GO:0007186	G-protein coupled receptor signaling pathway

InterPro

Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 359..379
None	No IPR available	COILS	Coil	Coil	coord: 454..457
None	No IPR available	GENE3D	G3DSA:1.20.1070.10		coord: 326..456 e-value: 1.7E-8 score: 35.7
None	No IPR available	GENE3D	G3DSA:1.20.1070.10		coord: 18..224 e-value: 4.3E-13 score: 50.9
None	No IPR available	PANTHER	PTHR24247	5-HYDROXYTRYPTAMINE RECEPTOR	coord: 27..450
None	No IPR available	CDD	cd15307	7tmA_5-HT2_insect-like	coord: 370..452 e-value: 1.15482E-4 score: 41.4759
None	No IPR available	CDD	cd15304	7tmA_5-HT2A	coord: 40..207 e-value: 2.13617E-11 score: 62.2572
None	No IPR available	SUPERFAMILY	SSF81321	Family A G protein-coupled receptor-like	coord: 20..453
None	No IPR available	TMHMM	TMhelix		coord: 25..47
None	No IPR available	TMHMM	TMhelix		coord: 139..161
None	No IPR available	TMHMM	TMhelix		coord: 59..81
None	No IPR available	TMHMM	TMhelix		coord: 96..118
None	No IPR available	TMHMM	TMhelix		coord: 380..402
None	No IPR available	TMHMM	TMhelix		coord: 422..438
None	No IPR available	TMHMM	TMhelix		coord: 181..203
IPR000276	G protein-coupled receptor, rhodopsin-like	PFAM	PF00001	7tm_1	coord: 40..432 e-value: 5.2E-18 score: 65.3
IPR017452	GPCR, rhodopsin-like, 7TM	PROSITE	PS50262	G_PROTEIN_RECEP_F1_2	coord: 39..435 score: 12.869

Main menu

SMED30023645

Zeng et. al., 2018

▻ Overview

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

Explore this single cell expression dataset with our NB Cluster Shiny App

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Davies et. al., 2017

PAGE et. al., 2020

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 4

Owlery

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