nucleolar protein 16

Overview
Namenucleolar protein 16
Smed IDSMED30023606
Length (bp)512
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of nucleolar protein 16 (SMED30023606) t-SNE clustered cells

Violin plots show distribution of expression levels for nucleolar protein 16 (SMED30023606) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of nucleolar protein 16 (SMED30023606) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for nucleolar protein 16 (SMED30023606) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30023606

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 4

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30023606h1SMcG0010288 SmedASXL_008218SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
nervous systemSMED30023606h1SMcG0010288 dd_Smed_v4_5060_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30023606h1SMcG0010288 dd_Smed_v4_5060_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30023606h1SMcG0010288 dd_Smed_v4_5060_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of nucleolar protein 16 vs. Ensembl Human
Match: NOP16 (NOP16 nucleolar protein [Source:HGNC Symbol;Acc:HGNC:26934])

HSP 1 Score: 78.9518 bits (193), Expect = 2.643e-18
Identity = 54/167 (32.34%), Postives = 88/167 (52.69%), Query Frame = 1 
Query:   43 KTRH--FSYDVNRKQLNRT--KKKQPKIIDETLKASWNSLKSTKQNLNEMGLVVDPNKLITLDG------GVNSSDTTTDIVKILKVKTTLKA----PKKEIILSEPDRI-LCLHFISNYGDDFKKMVKDDENIYQLTAGQIGQMVKKFKS--SKHYQEYLNLISTK 492
            KTR   F Y VNRK+LNR   +K  P+I    ++ +W+  KS +QNL EMGL VDPN+ + L         V+  +   ++V+   V   L+A    P+K+      D I    + + N+G+D+K M +D++N YQ T  QI   +  +K      +Q++L+ +  +
Sbjct:    7 KTRRQKFGYSVNRKRLNRNARRKAAPRIECSHIRHAWDHAKSVRQNLAEMGLAVDPNRAVPLRKRKVKAMEVDIEERPKELVRKPYVLNDLEAEASLPEKKGNTLSRDLIDYVRYMVENHGEDYKAMARDEKNYYQDTPKQIRSKINVYKRFYPAEWQDFLDSLQKR 173
[Close]
BLAST of nucleolar protein 16 vs. Ensembl Human
Match: NOP16 (NOP16 nucleolar protein [Source:HGNC Symbol;Acc:HGNC:26934])

HSP 1 Score: 60.8474 bits (146), Expect = 3.394e-12
Identity = 27/56 (48.21%), Postives = 37/56 (66.07%), Query Frame = 1 
Query:   55 FSYDVNRKQLNRT--KKKQPKIIDETLKASWNSLKSTKQNLNEMGLVVDPNKLITL 216
            F Y VNRK+LNR   +K  P+I    ++ +W+  KS +QNL EMGL VDPN+ + L
Sbjct:   13 FGYSVNRKRLNRNARRKAAPRIECSHIRHAWDHAKSVRQNLAEMGLAVDPNRAVPL 68
[Close]
BLAST of nucleolar protein 16 vs. Ensembl Human
Match: NOP16 (NOP16 nucleolar protein [Source:HGNC Symbol;Acc:HGNC:26934])

HSP 1 Score: 60.8474 bits (146), Expect = 1.121e-11
Identity = 43/125 (34.40%), Postives = 66/125 (52.80%), Query Frame = 1 
Query:   43 KTRH--FSYDVNRKQLNRT--KKKQPKIIDETLKASWNSLKSTKQNLNEMGLVVDPNKLITLDGG------VNSSDTTTDIVKILKVKTTLKA----PKKEIILSEPDRI-LCLHFISNYGDDFK 372
            KTR   F Y VNRK+LNR   +K  P+I    ++ +W+  KS +QNL EMGL VDPN+ + L         V+  +   ++V+   V   L+A    P+K+      D I    + + N+G+D+K
Sbjct:    7 KTRRQKFGYSVNRKRLNRNARRKAAPRIECSHIRHAWDHAKSVRQNLAEMGLAVDPNRAVPLRKRKVKAMEVDIEERPKELVRKPYVLNDLEAEASLPEKKGNTLSRDLIDYVRYMVENHGEDYK 131
[Close]
BLAST of nucleolar protein 16 vs. Ensembl Human
Match: NOP16 (NOP16 nucleolar protein [Source:HGNC Symbol;Acc:HGNC:26934])

HSP 1 Score: 60.8474 bits (146), Expect = 4.390e-11
Identity = 30/62 (48.39%), Postives = 40/62 (64.52%), Query Frame = 1 
Query:   43 KTRH--FSYDVNRKQLNRT--KKKQPKIIDETLKASWNSLKSTKQNLNEMGLVVDPNKLITL 216
            KTR   F Y VNRK+LNR   +K  P+I    ++ +W+  KS +QNL EMGL VDPN+ + L
Sbjct:    7 KTRRQKFGYSVNRKRLNRNARRKAAPRIECSHIRHAWDHAKSVRQNLAEMGLAVDPNRAVPL 68
[Close]
BLAST of nucleolar protein 16 vs. Ensembl Human
Match: NOP16 (NOP16 nucleolar protein [Source:HGNC Symbol;Acc:HGNC:26934])

HSP 1 Score: 60.8474 bits (146), Expect = 4.436e-11
Identity = 30/62 (48.39%), Postives = 40/62 (64.52%), Query Frame = 1 
Query:   43 KTRH--FSYDVNRKQLNRT--KKKQPKIIDETLKASWNSLKSTKQNLNEMGLVVDPNKLITL 216
            KTR   F Y VNRK+LNR   +K  P+I    ++ +W+  KS +QNL EMGL VDPN+ + L
Sbjct:    7 KTRRQKFGYSVNRKRLNRNARRKAAPRIECSHIRHAWDHAKSVRQNLAEMGLAVDPNRAVPL 68
[Close]
BLAST of nucleolar protein 16 vs. Ensembl Fly
Match: CG11563 (gene:FBgn0039868 transcript:FBtr0085831)

HSP 1 Score: 47.3654 bits (111), Expect = 9.657e-7
Identity = 39/166 (23.49%), Postives = 71/166 (42.77%), Query Frame = 1 
Query:   49 RHFSYDVNRKQLNRTKKKQPKIIDETLKASWNSLKSTKQNLNEMGLVVDPNKLITLDG-------------GVNSSDTTTDIVKILKVKTTL--KAPKKEIILSEPDRILC-----------------LHFISNYGDDFKKMVKDDENIYQLTAGQIGQMVKKFKS 450
            + + Y+VNRK +N+T+    KI D  +K  W   +    N +EMGL  D NK I +               G    +   D  ++L +K  +  + PK+  ++ E +++                    +F++ +  ++K MV D +N  Q T  Q    +++F S
Sbjct:   10 KRYRYNVNRKTMNKTRSSTGKIKDPRMKKMWMEDQRVGTNFSEMGLAKDVNKAIAIPSYKKDRLLAARVVNGFLEEELDEDERRVLGLKKPVVEEGPKRGHVVQELEQLASERADPEFRLPKGVVKELSYFLNKHKFNYKAMVADRKNFGQWTWRQFRLKIRRFMS 175
[Close]
BLAST of nucleolar protein 16 vs. Ensembl Zebrafish
Match: nop16 (NOP16 nucleolar protein homolog (yeast) [Source:NCBI gene;Acc:436807])

HSP 1 Score: 51.6026 bits (122), Expect = 3.006e-8
Identity = 34/140 (24.29%), Postives = 67/140 (47.86%), Query Frame = 1 
Query:  100 KQPKIIDETLKASWNSLKSTKQNLNEMGLVVDPNKLITLDGGVNS--SDTTTDIVKILKVKTTLKAPKKEIILSEPDRILC--------LHFISNYGDDFKKMVKDDENIYQLTAGQIGQMVKKFKSSKHYQEYLNLIST 489
            ++P I    ++ +W+  K+ KQNL +MGL      ++ +    N+      T+++K   ++      + E  +   DR  C         H +  + +D+K M +D++N YQ T  QI + V+ +K   H ++Y   I++
Sbjct:   30 EKPHIECPQIRKAWDEHKTVKQNLQDMGLAPGTKGMLPIKPKKNTKVEPMETNVLKPYVIEEM----EAEASVRGNDRTTCSTDMIEYVQHMVKEHNEDYKAMARDEKNYYQDTPKQIKRKVELYKRC-HPEQYAAFIAS 164
[Close]
BLAST of nucleolar protein 16 vs. Ensembl Xenopus
Match: lonrf2 (LON peptidase N-terminal domain and ring finger 2 [Source:Xenbase;Acc:XB-GENE-6045285])

HSP 1 Score: 72.7886 bits (177), Expect = 4.914e-16
Identity = 43/136 (31.62%), Postives = 75/136 (55.15%), Query Frame = 1 
Query:  106 PKIIDETLKASWNSLKSTKQNLNEMGLVVDPNKLITLDGGV--NSSDTTTDIVKILKVKTTLKA----PKKEIILSEPDRILCL-HFISNYGDDFKKMVKDDENIYQLTAGQIGQMVKKFKSSKHYQEYLNLISTK 492
            P+I  E ++++WN  KS  +NL +MGL  +PNK + +      ++ +    ++K   V   L+A    P K+ +    D I  + H + NYG+D+K M +D++N YQ T  QI + V  +K   H Q+Y  L++++
Sbjct:   31 PRIPCEPIRSAWNDGKSVAKNLADMGLAANPNKSLPIRPSQVKDAEEQPGKVIKKPYVLEGLQALASLPSKQTMGISSDMIQYVRHMVENYGEDYKAMARDEKNYYQDTPKQIQRKVNLYKRH-HPQDYQALVASR 165
[Close]
BLAST of nucleolar protein 16 vs. Ensembl Mouse
Match: Nop16 (NOP16 nucleolar protein [Source:MGI Symbol;Acc:MGI:107862])

HSP 1 Score: 82.8037 bits (203), Expect = 7.519e-20
Identity = 56/167 (33.53%), Postives = 90/167 (53.89%), Query Frame = 1 
Query:   43 KTRH--FSYDVNRKQLNRT--KKKQPKIIDETLKASWNSLKSTKQNLNEMGLVVDPNKLITLDG------GVNSSDTTTDIVKILKVKTTLKA----PKKEIILSEPDRI-LCLHFISNYGDDFKKMVKDDENIYQLTAGQIGQMVKKFKS--SKHYQEYLNLISTK 492
            KTR   F Y+VNRK+LNR   +K  P+I    ++ +W+  KS +QNL EMGL +DPNK + L         V++ +   D+V+   V   L+A    P+K+      D I    + + N+G+D+K M +D++N YQ T  QI   +  +K      +Q +++ + TK
Sbjct:    7 KTRRQKFGYNVNRKRLNRNARRKAAPRIECSHIRHAWDHTKSVRQNLAEMGLAMDPNKAVPLRKKKVKAMEVDTEERPRDLVRKPYVVNDLEAEASLPEKKGNTLSRDLIDYVRYMVENHGEDYKAMARDEKNYYQDTPKQIRNKINVYKRFYPTEWQAFIDSLQTK 173
[Close]
BLAST of nucleolar protein 16 vs. Ensembl Mouse
Match: Nop16 (NOP16 nucleolar protein [Source:MGI Symbol;Acc:MGI:107862])

HSP 1 Score: 81.2629 bits (199), Expect = 2.415e-19
Identity = 55/167 (32.93%), Postives = 90/167 (53.89%), Query Frame = 1 
Query:   43 KTRH--FSYDVNRKQLNRT--KKKQPKIIDETLKASWNSLKSTKQNLNEMGLVVDPNKLITLDG------GVNSSDTTTDIVKILKVKTTLKA----PKKEIILSEPDRI-LCLHFISNYGDDFKKMVKDDENIYQLTAGQIGQMVKKFKS--SKHYQEYLNLISTK 492
            KTR   F Y+VNRK+LNR   +K  P+I    ++ +W+  KS +QNL EMGL +DPNK + L         V++ +   D+V+   V   L+A    P+K+      D I    + + N+G+D+K M +D++N YQ T  QI   +  +K      +Q +++ + +K
Sbjct:    7 KTRRQKFGYNVNRKRLNRNARRKAAPRIECSHIRHAWDHTKSVRQNLAEMGLAMDPNKAVPLRKKKVKAMEVDTEERPRDLVRKPYVVNDLEAEASLPEKKGNTLSRDLIDYVRYMVENHGEDYKAMARDEKNYYQDTPKQIRNKINVYKRFYPTEWQAFIDSLQSK 173
[Close]
BLAST of nucleolar protein 16 vs. UniProt/SwissProt
Match: sp|Q1RP77|NOP16_RAT (Nucleolar protein 16 OS=Rattus norvegicus OX=10116 GN=Nop16 PE=2 SV=1)

HSP 1 Score: 83.9593 bits (206), Expect = 1.510e-19
Identity = 54/150 (36.00%), Postives = 84/150 (56.00%), Query Frame = 1 
Query:   43 KTRH--FSYDVNRKQLNRT--KKKQPKIIDETLKASWNSLKSTKQNLNEMGLVVDPNKLITLDG------GVNSSDTTTDIVKILKVKTTLKA----PKKEIILSEPDRILCLHF-ISNYGDDFKKMVKDDENIYQLTAGQIGQMVKKFK 447
            KTR   F Y+VNRK+LNR   +K  P+I    ++ +W+  KS +QNL EMGL +DPNK + L         V++ +   D+V+   V   L+A    P+K+      D I  +H+ + N+G+D+K M +D++N YQ T  QI   +  +K
Sbjct:    7 KTRRQKFGYNVNRKRLNRNARRKAAPRIECSHIRHAWDHTKSVRQNLAEMGLAMDPNKAVPLRKRKVKAMEVDTEERPKDLVRKPYVVNDLEAEASLPEKKGNTLSRDLIDYVHYMVENHGEDYKAMARDEKNYYQDTPKQIRNKINVYK 156
[Close]
BLAST of nucleolar protein 16 vs. UniProt/SwissProt
Match: sp|Q9CPT5|NOP16_MOUSE (Nucleolar protein 16 OS=Mus musculus OX=10090 GN=Nop16 PE=1 SV=1)

HSP 1 Score: 81.2629 bits (199), Expect = 1.689e-18
Identity = 55/167 (32.93%), Postives = 90/167 (53.89%), Query Frame = 1 
Query:   43 KTRH--FSYDVNRKQLNRT--KKKQPKIIDETLKASWNSLKSTKQNLNEMGLVVDPNKLITLDG------GVNSSDTTTDIVKILKVKTTLKA----PKKEIILSEPDRI-LCLHFISNYGDDFKKMVKDDENIYQLTAGQIGQMVKKFKS--SKHYQEYLNLISTK 492
            KTR   F Y+VNRK+LNR   +K  P+I    ++ +W+  KS +QNL EMGL +DPNK + L         V++ +   D+V+   V   L+A    P+K+      D I    + + N+G+D+K M +D++N YQ T  QI   +  +K      +Q +++ + +K
Sbjct:    7 KTRRQKFGYNVNRKRLNRNARRKAAPRIECSHIRHAWDHTKSVRQNLAEMGLAMDPNKAVPLRKKKVKAMEVDTEERPRDLVRKPYVVNDLEAEASLPEKKGNTLSRDLIDYVRYMVENHGEDYKAMARDEKNYYQDTPKQIRNKINVYKRFYPTEWQAFIDSLQSK 173
[Close]
BLAST of nucleolar protein 16 vs. UniProt/SwissProt
Match: sp|Q9Y3C1|NOP16_HUMAN (Nucleolar protein 16 OS=Homo sapiens OX=9606 GN=NOP16 PE=1 SV=2)

HSP 1 Score: 78.9518 bits (193), Expect = 1.269e-17
Identity = 54/167 (32.34%), Postives = 88/167 (52.69%), Query Frame = 1 
Query:   43 KTRH--FSYDVNRKQLNRT--KKKQPKIIDETLKASWNSLKSTKQNLNEMGLVVDPNKLITLDG------GVNSSDTTTDIVKILKVKTTLKA----PKKEIILSEPDRI-LCLHFISNYGDDFKKMVKDDENIYQLTAGQIGQMVKKFKS--SKHYQEYLNLISTK 492
            KTR   F Y VNRK+LNR   +K  P+I    ++ +W+  KS +QNL EMGL VDPN+ + L         V+  +   ++V+   V   L+A    P+K+      D I    + + N+G+D+K M +D++N YQ T  QI   +  +K      +Q++L+ +  +
Sbjct:    7 KTRRQKFGYSVNRKRLNRNARRKAAPRIECSHIRHAWDHAKSVRQNLAEMGLAVDPNRAVPLRKRKVKAMEVDIEERPKELVRKPYVLNDLEAEASLPEKKGNTLSRDLIDYVRYMVENHGEDYKAMARDEKNYYQDTPKQIRSKINVYKRFYPAEWQDFLDSLQKR 173
[Close]
BLAST of nucleolar protein 16 vs. UniProt/SwissProt
Match: sp|Q6GP80|NOP16_XENLA (Nucleolar protein 16 OS=Xenopus laevis OX=8355 GN=nop16 PE=2 SV=1)

HSP 1 Score: 75.8702 bits (185), Expect = 1.680e-16
Identity = 45/135 (33.33%), Postives = 75/135 (55.56%), Query Frame = 1 
Query:  106 PKIIDETLKASWNSLKSTKQNLNEMGLVVDPNKLITLDGG--VNSSDTTTDIVKILKVKTTLKA----PKKEIILSEPDRI-LCLHFISNYGDDFKKMVKDDENIYQLTAGQIGQMVKKFKSSKHYQEYLNLIST 489
            P+I  ET++ +WN  KS  +NL EMGL  DPNK + +      ++ +  ++++K   V   L+A    P K+ +    D I    H + NYG+D+K M +D++N YQ T  QI + V  +K   H Q++  +I++
Sbjct:   31 PRIPCETIRNAWNDGKSVAKNLAEMGLAADPNKCLPVRPSKVKDAEEQHSNVIKKPYVLEGLQALASLPSKQTMGISSDMIHYVRHMVENYGEDYKAMARDEKNYYQDTPKQIQRKVNLYKRH-HPQDFQAMITS 164
[Close]
BLAST of nucleolar protein 16 vs. UniProt/SwissProt
Match: sp|Q5E996|NOP16_BOVIN (Nucleolar protein 16 OS=Bos taurus OX=9913 GN=NOP16 PE=2 SV=1)

HSP 1 Score: 75.0998 bits (183), Expect = 3.636e-16
Identity = 50/150 (33.33%), Postives = 81/150 (54.00%), Query Frame = 1 
Query:   43 KTRH--FSYDVNRKQLNRT--KKKQPKIIDETLKASWNSLKSTKQNLNEMGLVVDPNKLITL------DGGVNSSDTTTDIVKILKVKTTLKA----PKKEIILSEPDRI-LCLHFISNYGDDFKKMVKDDENIYQLTAGQIGQMVKKFK 447
            KTR   F Y+VNRK+LNR   +K  P+I    ++ +W+  KS +QNL EMGL +DPN+ + L         V+  +   ++V+   V   L+A    P+K+      D I    + + N+G+++K M +D++N YQ T  QI   +  +K
Sbjct:    7 KTRRQKFGYNVNRKRLNRNARRKAAPRIECSHIRHAWDQTKSVRQNLAEMGLAMDPNRAVPLLKRKVKAMEVDVEERPKELVRKPYVLNDLEAEASLPEKKGNTLSRDLIDYVRYMVENHGENYKAMARDEKNYYQDTPKQIRNKINVYK 156
[Close]
BLAST of nucleolar protein 16 vs. TrEMBL
Match: A0A068Y6M0 (Ribosome biolocus tagsis protein Nop16 OS=Echinococcus multilocularis OX=6211 GN=EmuJ_000800900 PE=4 SV=1)

HSP 1 Score: 92.4337 bits (228), Expect = 1.506e-20
Identity = 52/144 (36.11%), Postives = 81/144 (56.25%), Query Frame = 1 
Query:   55 FSYDVNRKQLNRTKKKQPKIIDETLKASWNSLKSTKQNLNEMGLVVDPNKLITLDGGVNSSDTT---TDIVKILKVKTTLKAPKK-EIILSEPDRILCLHFISNYGDDFKKMVKDDENIYQLTAGQIGQMVKKFKSSKHYQEYL 474
            + Y  NRK+  R K K+  + +  ++ SWN   S + N N +GLV DPN+  T    V+  +      D   I ++K   K+ +K E  +SE DRI C++ +  YG+D+  M +D  N+YQLT  QI +M++ F+ SK+Y  YL
Sbjct:    7 YDYRKNRKKSLRKKYKRFIVQNSIIRKSWNDRLSVRANFNRIGLVFDPNE--TTCNTVSRKEEVEGLADPSVIRELKNMKKSKRKRERFISEDDRIFCIYLLELYGEDYGAMCRDSRNVYQLTPTQIERMIRIFRESKYYPRYL 148
[Close]
BLAST of nucleolar protein 16 vs. TrEMBL
Match: W6UTA8 (Nucleolar protein OS=Echinococcus granulosus OX=6210 GN=EGR_00129 PE=4 SV=1)

HSP 1 Score: 91.6633 bits (226), Expect = 3.860e-20
Identity = 45/122 (36.89%), Postives = 70/122 (57.38%), Query Frame = 1 
Query:  112 IIDETLKASWNSLKSTKQNLNEMGLVVDPNKLITLDGGVNSSDTTTDIVKILKVKTTLKAPKK-EIILSEPDRILCLHFISNYGDDFKKMVKDDENIYQLTAGQIGQMVKKFKSSKHYQEYL 474
            I +  ++ SWN   S + N N +GLV DPN+     G  +  +   D   I ++K   K+ +K E  +SE DRI C++ +  YG+D+  M +D  N+YQLT  QI +M++ F+ SK+Y  YL
Sbjct:   26 IQNSIIRKSWNDRLSIRANFNRIGLVFDPNESTCNTGRKDEVEGLADPNVIRELKNVRKSKRKREKFISEDDRIFCIYLLELYGEDYGAMCRDSRNVYQLTPTQIERMIRIFRESKYYPRYL 147
[Close]
BLAST of nucleolar protein 16 vs. TrEMBL
Match: W4ZFK6 (Uncharacterized protein OS=Strongylocentrotus purpuratus OX=7668 PE=4 SV=1)

HSP 1 Score: 89.7373 bits (221), Expect = 3.512e-19
Identity = 54/165 (32.73%), Postives = 86/165 (52.12%), Query Frame = 1 
Query:   37 VKKTRHFSYDVNRKQLNRTKKKQPKIIDETLKASWNSLKSTKQNLNEMGLVVDPNKL------------ITLDGGVNSSDTTT------DIVKILKVKTTLKAPKKEIILSEPDRILCLHFISNYGDDFKKMVKDDENIYQLTAGQIGQMVKKFKSSK-HYQEYL 474
             ++ + + Y  NR+++ + +K+ P I  + +K +W++ KS K+NL  MGL  DPNK             ++LD  ++  D          ++K L+    +   K+E I SEP  + C H +  YG DFK M +D +N YQ T  QI + +  FK+ K  YQEYL
Sbjct:    7 ARQRKKYKYSANRRKMWKKQKRLPNIECDPIKTAWDNKKSVKENLRAMGLSSDPNKTLPIPTTKERLAPVSLDDSMDFDDKLARPKGKQQVIKELEAMADMPTKKREKI-SEPMMMYCAHMMDKYGTDFKAMARDIKNYYQDTPKQIKKKIALFKTCKVQYQEYL 170
[Close]
BLAST of nucleolar protein 16 vs. TrEMBL
Match: A0A210QRQ7 (Nucleolar protein 16 OS=Mizuhopecten yessoensis OX=6573 GN=KP79_PYT16898 PE=4 SV=1)

HSP 1 Score: 88.9669 bits (219), Expect = 5.659e-19
Identity = 56/162 (34.57%), Postives = 86/162 (53.09%), Query Frame = 1 
Query:   43 KTRHFSYDVNRKQLNRTKKKQPKIIDETLKASWNSLKSTKQNLNEMGLVVDPNK----------LIT-LDGGVNSSDTTTDIVK-----ILKVKTTLKAP-KKEIILSEPDRILCLHFISNYGDDFKKMVKDDENIYQLTAGQIGQMVKKFKS-SKHYQEYL 474
            + + F+YD NRK+     KK P I  + ++ +W+S +S ++N+ EMGL  DPNK          LIT + G     +   D V       L+++     P K+ + LSEP+   C++ +  YG+D+K M +D +N YQ T  QI   +K FKS  + Y EYL
Sbjct:    9 RKKKFNYDQNRKKQWMKSKKMPTIECKQIRNAWDSTRSMQENMKEMGLATDPNKTLKVPKTKDFLITSIPGTSKKREKIEDSVPTKQYVALELEAEANIPQKRRLRLSEPETRYCIYMMERYGEDYKAMARDIKNYYQDTPKQIKAKIKAFKSIPEQYGEYL 170
[Close]
BLAST of nucleolar protein 16 vs. TrEMBL
Match: A0A3P7LNR1 (Uncharacterized protein OS=Dibothriocephalus latus OX=60516 GN=DILT_LOCUS7419 PE=4 SV=1)

HSP 1 Score: 88.1965 bits (217), Expect = 7.533e-19
Identity = 54/144 (37.50%), Postives = 87/144 (60.42%), Query Frame = 1 
Query:   55 FSYDVNRKQLNRTKKKQPKIIDETLKASWNSLKSTKQNLNEMGLVVDPNKLIT-LDGGVNSSD-TTTDIVKILK-VKTTLKAPKKEIILSEPDRILCLHFISNYGDDFKKMVKDDENIYQLTAGQIGQMVKKFKSSKHYQEYLN 477
            F Y  +R++  R +KK   I +  L+ SW    S K N  ++GL  +PN+ +  L+  V S   T T  VK LK VK+  K  ++E  +SE DR+  ++ I  YGDD K M +D +N+YQLT  QI +++++F+SS ++++YL+
Sbjct:    6 FDYTKDRRKQWRKEKKPIVIKNPILQKSWAPNMSVKANFEKLGLAFNPNQAVGDLESEVKSQFLTETSTVKELKKVKSCSK--QREKFISEDDRLFSMYMIELYGDDHKAMSRDPKNVYQLTPTQIRRLIERFRSSHYFKDYLD 147
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BLAST of nucleolar protein 16 vs. Ensembl Cavefish
Match: nop16 (NOP16 nucleolar protein homolog (yeast) [Source:NCBI gene;Acc:436807])

HSP 1 Score: 46.595 bits (109), Expect = 1.473e-6
Identity = 31/138 (22.46%), Postives = 66/138 (47.83%), Query Frame = 1 
Query:  100 KQPKIIDETLKASWNSLKSTKQNLNEMGLVVDPNKLITLDGGVNSSDTTTD--------IVKILKVKTTLKAPKKEIILSEPDRILCLHFISNYGDDFKKMVKDDENIYQLTAGQIGQMVKKFKSSKHYQEYLNLIST 489
            ++P I    ++ +W+  KS KQN+ +MGL      ++ ++    S+             +++ ++   +L+        ++    +  + I  + +D+K M KD++N YQ T  QI + V+ +K   H Q+Y   I++
Sbjct:   30 ERPTIGCAQIRKAWDDRKSAKQNMQDMGLSYGTKGVLPINKKSFSAPVEEAQAVVVKPYVIREMETAASLRGKNTRTCSNDLTEFV-QYMIREHNEDYKAMAKDEKNYYQHTPKQIKRKVELYKRC-HPQDYGAFIAS 165
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BLAST of nucleolar protein 16 vs. Ensembl Nematostella
Match: EDO47617 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RLJ9])

HSP 1 Score: 58.9214 bits (141), Expect = 1.280e-11
Identity = 42/133 (31.58%), Postives = 66/133 (49.62%), Query Frame = 1 
Query:  112 IIDETLKASWNSLKSTKQNLNEMGLVVDPNKLITLDGGVNSSD---------TTTDIVKILKVKTTLKAP--KKEIILSEPDRILCLHFISNYGDDFKKMVKDDENIYQLTAGQIGQMVKKF-KSSKHYQEYL 474
            I++ TL   W+  K+ KQN  E+GLV D N  + +       D         + TD+V+  +   TL A   KKE  ++  +       I  +G+D+K M KD +N YQ T  Q+ +  + F +SS+ + EYL
Sbjct:   15 IMNSTLSKRWDHSKTLKQNFQELGLVSDVNVALPIQKKKKREDDNEKMEVEKSPTDVVQEFE---TLAANEVKKERRIAPGEAHFVWKLIQKHGEDYKAMSKDKDNYYQHTPKQLKRKCEAFLRSSQDFSEYL 144
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BLAST of nucleolar protein 16 vs. Ensembl Medaka
Match: nop16 (NOP16 nucleolar protein [Source:NCBI gene;Acc:101167106])

HSP 1 Score: 63.929 bits (154), Expect = 6.094e-13
Identity = 44/153 (28.76%), Postives = 77/153 (50.33%), Query Frame = 1 
Query:   55 FSYDVNRKQLNR--TKKKQPKIIDETLKASWNSLKSTKQNLNEMGLVVDPNKLITLDGGVNSSDTTTDIVK---ILKVKTTLKAPKKEIILSEPDRILCLHF-ISNYGDDFKKMVKDDENIYQLTAGQIGQMVKKFKSS--KHYQEYLNLIST 489
            F Y  NRK L R   KK +P I D  ++ +W+  KST  NL  MGL  DPN+ + +    ++      I K   + +++     P+K+      D +  + + +  + DD+K M +D++N YQ T  QI + + ++K    + +Q +LN +  
Sbjct:   13 FDYSKNRKNLKRKFQKKSRPWIEDSHIRNAWDDSKSTASNLLAMGLSSDPNRTLPVKKTRSAPQRAGIITKPYILNQLEEEASLPEKDCKTLSSDLMEYVQYMVRQHKDDYKAMARDEKNYYQDTPKQIKRKINEYKRCHPQLFQAFLNSLGA 165
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BLAST of nucleolar protein 16 vs. Planmine SMEST
Match: SMESG000078630.1 (SMESG000078630.1)

HSP 1 Score: 310.457 bits (794), Expect = 3.789e-110
Identity = 153/153 (100.00%), Postives = 153/153 (100.00%), Query Frame = 1 
Query:   34 MVKKTRHFSYDVNRKQLNRTKKKQPKIIDETLKASWNSLKSTKQNLNEMGLVVDPNKLITLDGGVNSSDTTTDIVKILKVKTTLKAPKKEIILSEPDRILCLHFISNYGDDFKKMVKDDENIYQLTAGQIGQMVKKFKSSKHYQEYLNLISTK 492
            MVKKTRHFSYDVNRKQLNRTKKKQPKIIDETLKASWNSLKSTKQNLNEMGLVVDPNKLITLDGGVNSSDTTTDIVKILKVKTTLKAPKKEIILSEPDRILCLHFISNYGDDFKKMVKDDENIYQLTAGQIGQMVKKFKSSKHYQEYLNLISTK
Sbjct:    1 MVKKTRHFSYDVNRKQLNRTKKKQPKIIDETLKASWNSLKSTKQNLNEMGLVVDPNKLITLDGGVNSSDTTTDIVKILKVKTTLKAPKKEIILSEPDRILCLHFISNYGDDFKKMVKDDENIYQLTAGQIGQMVKKFKSSKHYQEYLNLISTK 153
The following BLAST results are available for this feature:
BLAST of nucleolar protein 16 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
NOP162.643e-1832.34NOP16 nucleolar protein [Source:HGNC Symbol;Acc:HG... [more]
NOP163.394e-1248.21NOP16 nucleolar protein [Source:HGNC Symbol;Acc:HG... [more]
NOP161.121e-1134.40NOP16 nucleolar protein [Source:HGNC Symbol;Acc:HG... [more]
NOP164.390e-1148.39NOP16 nucleolar protein [Source:HGNC Symbol;Acc:HG... [more]
NOP164.436e-1148.39NOP16 nucleolar protein [Source:HGNC Symbol;Acc:HG... [more]
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BLAST of nucleolar protein 16 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of nucleolar protein 16 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 1
Match NameE-valueIdentityDescription
CG115639.657e-723.49gene:FBgn0039868 transcript:FBtr0085831[more]
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BLAST of nucleolar protein 16 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
nop163.006e-824.29NOP16 nucleolar protein homolog (yeast) [Source:NC... [more]
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BLAST of nucleolar protein 16 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1
Match NameE-valueIdentityDescription
lonrf24.914e-1631.62LON peptidase N-terminal domain and ring finger 2 ... [more]
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BLAST of nucleolar protein 16 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 2
Match NameE-valueIdentityDescription
Nop167.519e-2033.53NOP16 nucleolar protein [Source:MGI Symbol;Acc:MGI... [more]
Nop162.415e-1932.93NOP16 nucleolar protein [Source:MGI Symbol;Acc:MGI... [more]
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BLAST of nucleolar protein 16 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q1RP77|NOP16_RAT1.510e-1936.00Nucleolar protein 16 OS=Rattus norvegicus OX=10116... [more]
sp|Q9CPT5|NOP16_MOUSE1.689e-1832.93Nucleolar protein 16 OS=Mus musculus OX=10090 GN=N... [more]
sp|Q9Y3C1|NOP16_HUMAN1.269e-1732.34Nucleolar protein 16 OS=Homo sapiens OX=9606 GN=NO... [more]
sp|Q6GP80|NOP16_XENLA1.680e-1633.33Nucleolar protein 16 OS=Xenopus laevis OX=8355 GN=... [more]
sp|Q5E996|NOP16_BOVIN3.636e-1633.33Nucleolar protein 16 OS=Bos taurus OX=9913 GN=NOP1... [more]
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BLAST of nucleolar protein 16 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A068Y6M01.506e-2036.11Ribosome biolocus tagsis protein Nop16 OS=Echinoco... [more]
W6UTA83.860e-2036.89Nucleolar protein OS=Echinococcus granulosus OX=62... [more]
W4ZFK63.512e-1932.73Uncharacterized protein OS=Strongylocentrotus purp... [more]
A0A210QRQ75.659e-1934.57Nucleolar protein 16 OS=Mizuhopecten yessoensis OX... [more]
A0A3P7LNR17.533e-1937.50Uncharacterized protein OS=Dibothriocephalus latus... [more]
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BLAST of nucleolar protein 16 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
nop161.473e-622.46NOP16 nucleolar protein homolog (yeast) [Source:NC... [more]
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BLAST of nucleolar protein 16 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of nucleolar protein 16 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of nucleolar protein 16 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO476171.280e-1131.58Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of nucleolar protein 16 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
nop166.094e-1328.76NOP16 nucleolar protein [Source:NCBI gene;Acc:1011... [more]
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BLAST of nucleolar protein 16 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000078630.13.789e-110100.00SMESG000078630.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30023606 ID=SMED30023606|Name=nucleolar protein 16|organism=Schmidtea mediterranea sexual|type=transcript|length=512bp
GTTTTAATATTCAGTATAGTTTGAAATTTTTTAATGGTAAAAAAAACTAG
GCATTTTAGTTACGATGTAAATCGTAAGCAATTAAACCGAACTAAAAAGA
AACAACCAAAAATTATTGACGAAACTTTAAAAGCGTCATGGAATAGTTTA
AAATCAACTAAACAAAATTTAAATGAAATGGGTTTGGTAGTTGATCCAAA
TAAACTTATAACACTAGATGGAGGTGTTAATTCTTCAGACACAACAACTG
ATATTGTAAAAATTCTTAAAGTTAAAACAACATTGAAAGCACCAAAAAAA
GAAATAATTCTTTCTGAACCTGATAGGATTTTATGTCTACATTTTATTTC
CAATTATGGCGATGATTTCAAAAAAATGGTTAAAGACGATGAAAATATCT
ATCAGCTAACAGCAGGACAAATTGGTCAAATGGTTAAGAAATTTAAATCT
TCAAAGCATTATCAAGAATATTTGAATTTAATATCAACTAAATAAATTTT
GCTTAATGAAAA
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protein sequence of SMED30023606-orf-1

>SMED30023606-orf-1 ID=SMED30023606-orf-1|Name=SMED30023606-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=154bp
MVKKTRHFSYDVNRKQLNRTKKKQPKIIDETLKASWNSLKSTKQNLNEMG
LVVDPNKLITLDGGVNSSDTTTDIVKILKVKTTLKAPKKEIILSEPDRIL
CLHFISNYGDDFKKMVKDDENIYQLTAGQIGQMVKKFKSSKHYQEYLNLI
STK*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0002109X1 cell
Vocabulary: INTERPRO
TermDefinition
IPR019002Ribosome_biogenesis_Nop16
Vocabulary: cellular component
TermDefinition
GO:0005634nucleus
GO:0005730nucleolus