SMED30023576

Overview
NameSMED30023576
Smed IDSMED30023576
Length (bp)3725
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30023576 (SMED30023576) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30023576 (SMED30023576) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30023576 (SMED30023576) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30023576 (SMED30023576) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30023576

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 15

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30023576SMESG000070794.1 SmedASXL_005585SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
head regionSMED30023576SMESG000070794.1 SMESG000070793.1 SmedASXL_003738SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
head regionSMED30023576SMESG000070794.1 SmedASXL_019821SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
head regionSMED30023576SMESG000070794.1 SmedASXL_000182SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
head regionSMED30023576SMESG000070796.1 SmedASXL_001534SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
head regionSMED30023576SMESG000070794.1 SmedASXL_003715SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
head regionSMED30023576SMESG000070794.1 SmedASXL_007669SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
nervous systemSMED30023576SMESG000070794.1 dd_Smed_v4_65314_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30023576SMESG000070794.1 dd_Smed_v4_65314_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30023576SMESG000070794.1 dd_Smed_v4_65314_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30023576SMESG000070793.1 dd_Smed_v4_39506_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30023576SMESG000070793.1 dd_Smed_v4_39506_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30023576SMESG000070793.1 dd_Smed_v4_39506_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30023576SMESG000070793.1 dd_Smed_v4_39506_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30023576SMESG000070793.1 dd_Smed_v6_39506_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
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BLAST of SMED30023576 vs. Planmine SMEST
Match: SMESG000070794.1 (SMESG000070794.1)

HSP 1 Score: 874.004 bits (2257), Expect = 0.000e+0
Identity = 492/496 (99.19%), Postives = 496/496 (100.00%), Query Frame = 1 
Query: 1441 SVILVGTIAXXXXXXXXXXXXXXXXXXXXXXRIWNNSKSKSNDRLCVQLVIDQSKWLFTNVCFAVSIIPRVYYMETDSILNINETMIIFHGENLISSFIYFCKLKCPEMDIIIMPCVVISKSKSLKCEASEVSLNKFLVPCRARIFIDTGKPISIEFPIQLFKVTEIFPMDQSIRKIIIETPKSTNNSIKQGKLYTKSISFQLILKSTVKVLKASFIRQIEKEINLICGKLICYNVKIINNVLNCWFSLSSIEHGYSCNIELQSSKLSFKTKNRLIFIKNIENSGKXXXXXXXXXXXXXXXXXXXXXXXXXXXTRYYQKHHRYQVINHPINASRYCDIAFFHHQNLETGIEENQNFQEISLKTNFKKFNSHKTFIEDLIINHITFNLSSTKYFFDMIERNEIYFLNKSVLEITLNKLLMNITTMKRIINFISTEPFQLTDIYKKEFLSLLSYLISVNSNVHFQLILSTLDLINNLLYQNYFLKNWIAFQIFNELKP 2928
            SVILVGTIASIKCIIKKISEKSIKCKIKSISRIWNNSKSKSNDRLCVQLVIDQSKWLFTNVCFAVSIIPRVYYMETDSILNINETMIIFHGENLISSFIYFCKLKCPEMDIIIMPC+VISKSKSLKCEASEVSLNKFLVPCRARIFIDTGKPISIEFPIQLFKVTEIFPMDQSIRKIIIETPKSTNNSIKQGKLYTKSISFQLILKSTVKVLKASFIRQIEKEINLICGKLICYNVKIINNVLNCWFSLSSIEHGYSCNIELQSSKLSFKTKNRLIFIKNIENSGKISIDSSTVLILVILVISLAIIIGIIILTRYYQKHHRYQVINHPINASRYCDIAFFHHQNLETGIEENQNFQEISLKTNFKKFNSHKTFIEDLIINHITFNLSSTKYFFDMIERNEIYF+NKSVLEITLNKLLMN+TTMKRIINFISTEPFQLTDIYKKEFLSLLSYLISVNSNVHFQLILST+DLINNLLYQNYFLKNWIAFQIFNELKP
Sbjct:  356 SVILVGTIASIKCIIKKISEKSIKCKIKSISRIWNNSKSKSNDRLCVQLVIDQSKWLFTNVCFAVSIIPRVYYMETDSILNINETMIIFHGENLISSFIYFCKLKCPEMDIIIMPCIVISKSKSLKCEASEVSLNKFLVPCRARIFIDTGKPISIEFPIQLFKVTEIFPMDQSIRKIIIETPKSTNNSIKQGKLYTKSISFQLILKSTVKVLKASFIRQIEKEINLICGKLICYNVKIINNVLNCWFSLSSIEHGYSCNIELQSSKLSFKTKNRLIFIKNIENSGKISIDSSTVLILVILVISLAIIIGIIILTRYYQKHHRYQVINHPINASRYCDIAFFHHQNLETGIEENQNFQEISLKTNFKKFNSHKTFIEDLIINHITFNLSSTKYFFDMIERNEIYFVNKSVLEITLNKLLMNVTTMKRIINFISTEPFQLTDIYKKEFLSLLSYLISVNSNVHFQLILSTVDLINNLLYQNYFLKNWIAFQIFNELKP 851

HSP 2 Score: 724.161 bits (1868), Expect = 0.000e+0
Identity = 353/355 (99.44%), Postives = 353/355 (99.44%), Query Frame = 2 
Query:  377 MDYNDPLPFLGEIIDLPFKNQMKLLSVLNIDGQIVTFSGDIIGLIYTHVKKENYLMLIQQRKIFSTPIEKIEIDNKNNLMIVASVYQIKVISVFDCRDYSNSEVCHGNIFCYWNIIQCRCVYFTQTMITNNFICEIEHKYIRSNSFGMVHLLSSFEEIVRYNPGIRINLIQNISKNLIVWTVKFGEASKTGILFPLLKPCNQCILRKSILNERFPINSTGNFKIPIILKIVPFRIAYNSCPKLTVTIDNFSNEFRNFYQNITFIITQSYKCKFIDASWNFPFTFQCQLPKISIKYSTPNHGIFIIINPLLSIQAIYSPILLLEPVVNKIKTRVGPLIDNREIMLRGNYLNVGNHH 1441
            MDYNDPLPFLGEIIDLPFKNQMKLLSVLNIDGQIVTFSGDIIGLIYTHVKKENYLMLIQQRKIFSTPIEKIEIDNKNNLMIVASVYQIKVISVFDCRDYSNSEVCHGNIFCYWNIIQCRCVYFTQTMITNNFICEIEHKYIRSNSFGMVHLLSSFEEIVRYNPGIRINLIQNISKNLIVWTVKFGEASKTGILFPLLKPCNQCILRKSILNERFPINSTGNFKIPIILKIVPFRIAYNSCPKLTVTIDNFSNEFRNFYQNITFIITQSYKCKFIDASWNFPFTFQCQLPKISIKYSTPNHGI IIINPLLSIQAIYSPILLLEPVVNKIKTRVGPLIDNREIMLRGNYLNVGNH 
Sbjct:    1 MDYNDPLPFLGEIIDLPFKNQMKLLSVLNIDGQIVTFSGDIIGLIYTHVKKENYLMLIQQRKIFSTPIEKIEIDNKNNLMIVASVYQIKVISVFDCRDYSNSEVCHGNIFCYWNIIQCRCVYFTQTMITNNFICEIEHKYIRSNSFGMVHLLSSFEEIVRYNPGIRINLIQNISKNLIVWTVKFGEASKTGILFPLLKPCNQCILRKSILNERFPINSTGNFKIPIILKIVPFRIAYNSCPKLTVTIDNFSNEFRNFYQNITFIITQSYKCKFIDASWNFPFTFQCQLPKISIKYSTPNHGILIIINPLLSIQAIYSPILLLEPVVNKIKTRVGPLIDNREIMLRGNYLNVGNHQ 355
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BLAST of SMED30023576 vs. Planmine SMEST
Match: SMESG000070793.1 (SMESG000070793.1)

HSP 1 Score: 342.813 bits (878), Expect = 1.507e-98
Identity = 167/170 (98.24%), Postives = 168/170 (98.82%), Query Frame = 1 
Query: 2953 MEFYFSMSKKISEETGSCYQNHLKKSATTTGDVQDHIQYISLDTQQLPKTLDNGETISQKVTVKIEEEYITSKIIKLFTKVFQTENVPEYLIYLYNTKSSTDNQTIFNELLTDIWQYIFENPTLFLGISGHVSYEIERQFKYISSLINLSKEFPENSVQDKILNHLYLKN 3462
            MEFYFSMSKKISEETGSCYQNHLKKS T TGDVQDHIQYISLDTQQLPKTLDNGETISQKVTVKIEEEYITSKIIKLFTKVFQTENVPEYLIYLYNTKSSTDNQTIFNELLT+IWQYIFENPTLFLGISGHVSYEIERQFKYISSLINLSKEFPENSVQDKILNHLYLKN
Sbjct:    1 MEFYFSMSKKISEETGSCYQNHLKKSPTATGDVQDHIQYISLDTQQLPKTLDNGETISQKVTVKIEEEYITSKIIKLFTKVFQTENVPEYLIYLYNTKSSTDNQTIFNELLTNIWQYIFENPTLFLGISGHVSYEIERQFKYISSLINLSKEFPENSVQDKILNHLYLKN 170
[Close]
BLAST of SMED30023576 vs. Planmine SMEST
Match: SMESG000070793.1 (SMESG000070793.1)

HSP 1 Score: 330.102 bits (845), Expect = 4.706e-94
Identity = 161/164 (98.17%), Postives = 162/164 (98.78%), Query Frame = 1 
Query: 2971 MSKKISEETGSCYQNHLKKSATTTGDVQDHIQYISLDTQQLPKTLDNGETISQKVTVKIEEEYITSKIIKLFTKVFQTENVPEYLIYLYNTKSSTDNQTIFNELLTDIWQYIFENPTLFLGISGHVSYEIERQFKYISSLINLSKEFPENSVQDKILNHLYLKN 3462
            MSKKISEETGSCYQNHLKKS T TGDVQDHIQYISLDTQQLPKTLDNGETISQKVTVKIEEEYITSKIIKLFTKVFQTENVPEYLIYLYNTKSSTDNQTIFNELLT+IWQYIFENPTLFLGISGHVSYEIERQFKYISSLINLSKEFPENSVQDKILNHLYLKN
Sbjct:    1 MSKKISEETGSCYQNHLKKSPTATGDVQDHIQYISLDTQQLPKTLDNGETISQKVTVKIEEEYITSKIIKLFTKVFQTENVPEYLIYLYNTKSSTDNQTIFNELLTNIWQYIFENPTLFLGISGHVSYEIERQFKYISSLINLSKEFPENSVQDKILNHLYLKN 164
[Close]
BLAST of SMED30023576 vs. Planmine SMEST
Match: SMESG000070793.1 (SMESG000070793.1)

HSP 1 Score: 268.855 bits (686), Expect = 1.379e-73
Identity = 133/156 (85.26%), Postives = 138/156 (88.46%), Query Frame = 1 
Query: 2953 MEFYFSMSKKISEETGSCYQNHLKKSATTTGDVQDHIQYISLDTQQLPKTLDNGETISQKVTVKIEEEYITSKIIKLFTKVFQTENVPEYLIYLYNTKSSTDNQTIFNELLTDIWQYIFENPTLFLGISGHVSYEIERQFKYISSLINLSKEFPEN 3420
            MEFYFSMSKKISEETGSCYQNHLKKS T TGDVQDHIQYISLDTQQLPKTLDNGETISQKVTVKIEEEYITSKIIKLFTKVFQTENVPEYLIYLYNTKSSTDNQTIFNELLT+IWQYIFENPTLFLGISGH           +S+L++  K   EN
Sbjct:    1 MEFYFSMSKKISEETGSCYQNHLKKSPTATGDVQDHIQYISLDTQQLPKTLDNGETISQKVTVKIEEEYITSKIIKLFTKVFQTENVPEYLIYLYNTKSSTDNQTIFNELLTNIWQYIFENPTLFLGISGHAQ---------VSNLVSFVKSLLEN 147
[Close]
BLAST of SMED30023576 vs. Planmine SMEST
Match: SMESG000070793.1 (SMESG000070793.1)

HSP 1 Score: 255.758 bits (652), Expect = 2.320e-69
Identity = 127/150 (84.67%), Postives = 132/150 (88.00%), Query Frame = 1 
Query: 2971 MSKKISEETGSCYQNHLKKSATTTGDVQDHIQYISLDTQQLPKTLDNGETISQKVTVKIEEEYITSKIIKLFTKVFQTENVPEYLIYLYNTKSSTDNQTIFNELLTDIWQYIFENPTLFLGISGHVSYEIERQFKYISSLINLSKEFPEN 3420
            MSKKISEETGSCYQNHLKKS T TGDVQDHIQYISLDTQQLPKTLDNGETISQKVTVKIEEEYITSKIIKLFTKVFQTENVPEYLIYLYNTKSSTDNQTIFNELLT+IWQYIFENPTLFLGISGH           +S+L++  K   EN
Sbjct:    1 MSKKISEETGSCYQNHLKKSPTATGDVQDHIQYISLDTQQLPKTLDNGETISQKVTVKIEEEYITSKIIKLFTKVFQTENVPEYLIYLYNTKSSTDNQTIFNELLTNIWQYIFENPTLFLGISGHAQ---------VSNLVSFVKSLLEN 141
The following BLAST results are available for this feature:
BLAST of SMED30023576 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023576 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023576 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023576 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023576 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023576 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023576 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023576 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023576 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023576 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023576 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023576 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023576 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023576 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000070794.10.000e+099.19SMESG000070794.1[more]
SMESG000070793.11.507e-9898.24SMESG000070793.1[more]
SMESG000070793.14.706e-9498.17SMESG000070793.1[more]
SMESG000070793.11.379e-7385.26SMESG000070793.1[more]
SMESG000070793.12.320e-6984.67SMESG000070793.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30023576 ID=SMED30023576|Name=SMED30023576|organism=Schmidtea mediterranea sexual|type=transcript|length=3725bp
CGATTTTGAAAGTTATGTTGAGATTCAACTGCAATGCTTTGATCAGAAAA
TTAATTTTGAAATCACATTATCTGACATCATCAGTCATTCTTCCTCCGAG
GGTCAATTTGAAAGTAAAATTTTAATTATTGGACTTCAAATGACAGAGAA
AATAAAAAATACAAATCAACAAAAAGCCTACTTGTACCATTTCCTAATGG
ATAAAATGATGAACCGATTCAATGAGGTTATGGAGAAATGTATAGTGAAA
GGAATTGGGTTTCAACAACCCCCTCATTTTAGCGGCAACGATGAGCCGAT
TAGATGTGAAAGTGAAAATTTTGCGAGAAATCCAAAGTGCAGCACTTTCA
ATTTGTATTCAAGAAATAGATTAATAATGGATTACAATGACCCTCTTCCT
TTTCTCGGTGAAATAATCGATTTACCATTTAAAAATCAAATGAAACTTCT
CTCAGTGTTGAATATTGACGGTCAAATTGTCACATTTTCCGGAGATATTA
TTGGATTAATATATACTCATGTCAAAAAAGAAAATTATTTGATGTTGATA
CAGCAACGAAAGATTTTCTCTACGCCGATAGAAAAAATAGAAATTGACAA
TAAAAATAATTTAATGATAGTAGCAAGTGTTTACCAAATAAAGGTTATCA
GCGTGTTTGATTGTCGAGATTATTCAAATTCTGAAGTGTGTCATGGGAAT
ATATTTTGCTATTGGAATATAATTCAATGCCGATGCGTTTATTTTACTCA
AACAATGATAACAAATAATTTTATTTGTGAAATTGAACATAAGTATATTA
GGTCAAATTCTTTTGGAATGGTTCATTTATTATCGTCATTTGAAGAAATT
GTTCGTTATAATCCAGGAATACGGATAAATTTAATTCAAAACATATCCAA
AAATTTAATTGTGTGGACTGTGAAATTTGGAGAAGCATCAAAAACGGGAA
TATTATTTCCACTTCTAAAACCGTGCAATCAATGTATTCTGAGAAAATCG
ATATTAAATGAGAGATTTCCAATAAATTCTACGGGAAATTTTAAAATTCC
AATTATTTTAAAAATTGTTCCATTCCGAATTGCATATAATTCTTGTCCAA
AATTGACAGTAACAATAGACAATTTTTCTAACGAATTCAGAAATTTTTAT
CAAAACATTACATTTATTATAACACAATCTTACAAATGTAAATTCATTGA
TGCATCTTGGAACTTTCCCTTTACTTTCCAATGCCAACTTCCGAAAATTT
CAATCAAATATTCAACTCCAAATCACGGGATTTTCATCATAATAAATCCT
CTTTTATCAATTCAAGCAATTTATTCTCCAATTTTATTATTAGAACCAGT
TGTCAATAAAATAAAGACTCGTGTTGGGCCATTGATAGACAACAGAGAAA
TTATGCTTCGGGGAAATTATCTTAACGTTGGTAATCACCATCTGTCATTC
TAGTTGGAACAATTGCATCTATCAAATGCATAATTAAAAAAATATCTGAA
AAATCAATAAAATGCAAAATCAAATCGATTTCACGAATTTGGAATAATTC
AAAATCGAAATCCAATGATCGATTGTGCGTTCAATTAGTAATCGACCAAT
CAAAATGGTTGTTTACCAATGTATGCTTTGCAGTCAGCATCATTCCCAGA
GTGTACTACATGGAAACTGATAGCATTTTAAATATAAATGAAACTATGAT
TATATTCCATGGAGAAAATCTCATTTCGAGTTTTATCTACTTTTGCAAAT
TGAAATGTCCCGAGATGGATATTATTATTATGCCTTGCGTCGTCATTTCA
AAATCAAAATCATTAAAATGTGAAGCGTCTGAAGTATCCCTAAACAAATT
TCTAGTGCCATGTAGAGCTAGAATATTTATTGATACTGGAAAACCAATTT
CAATTGAATTTCCCATTCAATTGTTTAAAGTCACAGAAATATTTCCTATG
GACCAAAGTATCAGGAAGATAATAATTGAAACTCCAAAGAGTACCAATAA
TTCAATAAAACAAGGAAAGCTGTATACAAAATCAATTTCCTTTCAATTAA
TTTTGAAATCGACAGTAAAGGTTTTGAAAGCTTCATTTATTCGACAAATA
GAAAAAGAAATAAATTTAATTTGTGGAAAATTGATTTGTTATAATGTGAA
AATTATCAATAATGTGCTCAATTGCTGGTTTTCACTGAGTTCAATTGAAC
ACGGATACTCGTGCAATATTGAATTACAAAGTTCAAAATTGTCATTTAAA
ACGAAAAATCGTTTGATATTTATTAAAAACATTGAAAATTCAGGAAAAAT
TTCTATAGATTCGTCTACAGTTTTGATTCTTGTAATTTTAGTGATCAGTT
TAGCAATTATAATAGGCATCATAATACTGACTCGGTACTATCAGAAACAT
CATAGATATCAAGTGATAAATCATCCAATCAACGCCAGCAGATATTGCGA
TATTGCATTTTTTCATCATCAAAATTTAGAAACTGGCATTGAGGAGAATC
AGAATTTTCAGGAAATTTCATTGAAAACCAATTTCAAAAAATTCAATTCA
CATAAGACTTTCATTGAAGATTTAATTATAAATCACATTACTTTTAATTT
GTCATCGACAAAGTATTTTTTTGATATGATTGAAAGGAATGAAATTTATT
TCCTCAATAAATCGGTTTTAGAAATTACCCTTAATAAATTACTGATGAAT
ATCACGACGATGAAACGAATAATAAATTTCATTTCAACAGAGCCATTTCA
GCTCACAGATATTTACAAAAAGGAATTTTTATCCTTGTTATCTTATTTAA
TTAGCGTTAATTCCAATGTTCATTTTCAATTGATTTTGTCAACCCTTGAT
CTTATTAATAATTTACTCTATCAGAATTATTTCTTAAAAAATTGGATTGC
ATTTCAGATTTTTAATGAACTTAAACCAGAATTAAAAACTCATAAATGCA
TCATGGAATTTTATTTTTCAATGTCGAAGAAAATCTCTGAAGAAACTGGC
TCCTGCTATCAGAACCATTTAAAAAAAAGTGCTACAACAACTGGAGATGT
GCAAGACCATATCCAATATATTTCTCTAGATACCCAACAACTTCCGAAAA
CTTTGGACAATGGTGAGACCATTTCTCAAAAGGTAACTGTCAAAATAGAG
GAAGAATATATTACATCAAAAATAATCAAATTGTTTACAAAAGTATTCCA
GACCGAAAATGTTCCAGAGTATTTAATTTATTTATATAACACGAAATCTT
CAACTGATAACCAAACAATTTTTAATGAATTATTGACTGATATTTGGCAA
TATATTTTTGAAAATCCGACATTATTTCTAGGAATATCTGGACATGTATC
TTATGAAATAGAAAGACAATTTAAATATATTAGTTCGTTAATAAATCTCT
CCAAGGAATTTCCTGAGAATTCTGTCCAAGATAAAATATTAAATCATTTA
TATTTGAAAAACATGTGCCAGAATATATTTTCACAATATATTATTTCATT
TACAATAAATAAAGTAGAAATAAAACATCAAATAAGTTCAGGGGCCTCCA
GGATCTCAGAATATCAAAATTATTGGAGCGTTTTAAATAAATTTTCCAAT
AATTACCTCAGTAAAATAGATATATGAAAAAATCTCAATGTTTTCGAGGA
ACTAAAACATTTTTTGAAAAAATTAATTTTCGTCGAAAATTTAATTCAAA
GGAACTAAAAATTTTTATTTGTATA
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protein sequence of SMED30023576-orf-1

>SMED30023576-orf-1 ID=SMED30023576-orf-1|Name=SMED30023576-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=484bp
DFESYVEIQLQCFDQKINFEITLSDIISHSSSEGQFESKILIIGLQMTEK
IKNTNQQKAYLYHFLMDKMMNRFNEVMEKCIVKGIGFQQPPHFSGNDEPI
RCESENFARNPKCSTFNLYSRNRLIMDYNDPLPFLGEIIDLPFKNQMKLL
SVLNIDGQIVTFSGDIIGLIYTHVKKENYLMLIQQRKIFSTPIEKIEIDN
KNNLMIVASVYQIKVISVFDCRDYSNSEVCHGNIFCYWNIIQCRCVYFTQ
TMITNNFICEIEHKYIRSNSFGMVHLLSSFEEIVRYNPGIRINLIQNISK
NLIVWTVKFGEASKTGILFPLLKPCNQCILRKSILNERFPINSTGNFKIP
IILKIVPFRIAYNSCPKLTVTIDNFSNEFRNFYQNITFIITQSYKCKFID
ASWNFPFTFQCQLPKISIKYSTPNHGIFIIINPLLSIQAIYSPILLLEPV
VNKIKTRVGPLIDNREIMLRGNYLNVGNHHLSF*
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protein sequence of SMED30023576-orf-2

>SMED30023576-orf-2 ID=SMED30023576-orf-2|Name=SMED30023576-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=656bp
METDSILNINETMIIFHGENLISSFIYFCKLKCPEMDIIIMPCVVISKSK
SLKCEASEVSLNKFLVPCRARIFIDTGKPISIEFPIQLFKVTEIFPMDQS
IRKIIIETPKSTNNSIKQGKLYTKSISFQLILKSTVKVLKASFIRQIEKE
INLICGKLICYNVKIINNVLNCWFSLSSIEHGYSCNIELQSSKLSFKTKN
RLIFIKNIENSGKISIDSSTVLILVILVISLAIIIGIIILTRYYQKHHRY
QVINHPINASRYCDIAFFHHQNLETGIEENQNFQEISLKTNFKKFNSHKT
FIEDLIINHITFNLSSTKYFFDMIERNEIYFLNKSVLEITLNKLLMNITT
MKRIINFISTEPFQLTDIYKKEFLSLLSYLISVNSNVHFQLILSTLDLIN
NLLYQNYFLKNWIAFQIFNELKPELKTHKCIMEFYFSMSKKISEETGSCY
QNHLKKSATTTGDVQDHIQYISLDTQQLPKTLDNGETISQKVTVKIEEEY
ITSKIIKLFTKVFQTENVPEYLIYLYNTKSSTDNQTIFNELLTDIWQYIF
ENPTLFLGISGHVSYEIERQFKYISSLINLSKEFPENSVQDKILNHLYLK
NMCQNIFSQYIISFTINKVEIKHQISSGASRISEYQNYWSVLNKFSNNYL
SKIDI*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000418head
PLANA:0002032epidermal cell
Vocabulary: INTERPRO
TermDefinition
IPR015943WD40/YVTN_repeat-like_dom_sf
Vocabulary: molecular function
TermDefinition
GO:0005515protein binding
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR015943WD40/YVTN repeat-like-containing domain superfamilyGENE3DG3DSA:2.130.10.10coord: 1..231
e-value: 3.6E-8
score: 34.7