SMED30023566

Overview
NameSMED30023566
Smed IDSMED30023566
Length (bp)1541
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30023566 (SMED30023566) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30023566 (SMED30023566) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30023566 (SMED30023566) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30023566 (SMED30023566) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30023566

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 6

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30023566h1SMcG0016062 SmedASXL_011614SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
muscle cellSMED30023566h1SMcG0016062 dd_Smed_v4_13599_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30023566h1SMcG0016062 dd_Smed_v4_13599_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
posterior sideSMED30023566h1SMcG0016062 dd_Smed_v4_13599_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
posterior muscle cellSMED30023566h1SMcG0016062 dd_Smed_v4_13599_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30023566h1SMcG0016062 dd_Smed_v6_13599_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of SMED30023566 vs. TrEMBL
Match: A0A0V0J772 (Protein eva-1 homolog C OS=Schistocephalus solidus OX=70667 GN=EVA1C PE=4 SV=1)

HSP 1 Score: 65.855 bits (159), Expect = 1.812e-7
Identity = 55/200 (27.50%), Postives = 87/200 (43.50%), Query Frame = 1 
Query:  259 SPINHSYSLSPYNCYFKYFENYILKTCANSFSCRICMNDIESVHTPNTQCKSEQDFLVKYRCVPG--YIKNENICTNSYLDIDCFDKLKEKLVIHKATLYPEIEKSEDSIGCPL--NSPKQC----RPVDKTYHVISKCSYRDKCSVGPHEY-----TSSMCKNMHLRLTYACVPEDVLENFMNEPSKEKPIKTKTKRFE 819
            +PI  + + S +    K   + I + C    SC I  +   +  +  T  K  +  LVKY C P    IK E +C ++YL++ C  +  + ++   A L   +E +  S  CP+    PK        +D + H+   C  R  CSV P          + C   HLRLTYAC    + EN    P+   P +TK ++ +
Sbjct:   82 TPIASAPATSSHCMNLKDVTSPIKQLCEGKLSCSISPDQFTANESSCTHMK--RHLLVKYICEPSPQAIKKEIVCLDTYLELKCGGRHPKDVI---AVLKASLEANTQSDRCPVLPEMPKMSTVCPATIDVSPHLRGLCDRRRTCSVMPDPSMLPTNQPASCGKRHLRLTYACANPSIFENDTPAPADSPPRRTKHRQGQ 276
[Close]
BLAST of SMED30023566 vs. TrEMBL
Match: A0A0X3Q1T3 (Protein eva-1 homolog C OS=Schistocephalus solidus OX=70667 GN=EVA1C PE=4 SV=1)

HSP 1 Score: 65.4698 bits (158), Expect = 1.942e-7
Identity = 55/200 (27.50%), Postives = 87/200 (43.50%), Query Frame = 1 
Query:  259 SPINHSYSLSPYNCYFKYFENYILKTCANSFSCRICMNDIESVHTPNTQCKSEQDFLVKYRCVPG--YIKNENICTNSYLDIDCFDKLKEKLVIHKATLYPEIEKSEDSIGCPL--NSPKQC----RPVDKTYHVISKCSYRDKCSVGPHEY-----TSSMCKNMHLRLTYACVPEDVLENFMNEPSKEKPIKTKTKRFE 819
            +PI  + + S +    K   + I + C    SC I  +   +  +  T  K  +  LVKY C P    IK E +C ++YL++ C  +  + ++   A L   +E +  S  CP+    PK        +D + H+   C  R  CSV P          + C   HLRLTYAC    + EN    P+   P +TK ++ +
Sbjct:   50 TPIASAPATSSHCMNLKDVTSPIKQLCEGKLSCSISPDQFTANESSCTHMK--RHLLVKYICEPSPQAIKKEIVCLDTYLELKCGGRHPKDVI---AVLKASLEANTQSDRCPVLPEMPKMSTVCPATIDVSPHLRGLCDRRRTCSVMPDPSMLPTNQPASCGKRHLRLTYACANPSIFENDTPAPADSPPRRTKHRQGQ 244
[Close]
BLAST of SMED30023566 vs. TrEMBL
Match: A0A0X3NXI2 (Protein eva-1 homolog C OS=Schistocephalus solidus OX=70667 GN=EVA1C PE=4 SV=1)

HSP 1 Score: 65.4698 bits (158), Expect = 2.099e-7
Identity = 55/200 (27.50%), Postives = 87/200 (43.50%), Query Frame = 1 
Query:  259 SPINHSYSLSPYNCYFKYFENYILKTCANSFSCRICMNDIESVHTPNTQCKSEQDFLVKYRCVPG--YIKNENICTNSYLDIDCFDKLKEKLVIHKATLYPEIEKSEDSIGCPL--NSPKQC----RPVDKTYHVISKCSYRDKCSVGPHEY-----TSSMCKNMHLRLTYACVPEDVLENFMNEPSKEKPIKTKTKRFE 819
            +PI  + + S +    K   + I + C    SC I  +   +  +  T  K  +  LVKY C P    IK E +C ++YL++ C  +  + ++   A L   +E +  S  CP+    PK        +D + H+   C  R  CSV P          + C   HLRLTYAC    + EN    P+   P +TK ++ +
Sbjct:   99 TPIASAPATSSHCMNLKDVTSPIKQLCEGKLSCSISPDQFTANESSCTHMK--RHLLVKYICEPSPQAIKKEIVCLDTYLELKCGGRHPKDVI---AVLKASLEANTQSDRCPVLPEMPKMSTVCPATIDVSPHLRGLCDRRRTCSVMPDPSMLPTNQPASCGKRHLRLTYACANPSIFENDTPAPADSPPRRTKHRQGQ 293
[Close]
BLAST of SMED30023566 vs. TrEMBL
Match: A0A068WKG0 (Pfam-B_8122 and DUF4381 domain containing protein OS=Echinococcus granulosus OX=6210 GN=EgrG_000591200 PE=4 SV=1)

HSP 1 Score: 62.3882 bits (150), Expect = 1.723e-6
Identity = 48/156 (30.77%), Postives = 73/156 (46.79%), Query Frame = 1 
Query:  421 DFLVKYRCVPG--YIKNENICTNSYLDIDCFD-KLKEKLVIHKATLY--------PEIEKSEDSIG-CPLNSPKQCRPVDKTYHVISKCSYRDKCSVGPHEYT-----SSMCKNMHLRLTYACVPEDVLEN-------FMNEPSKEK-PIKTKTKR 813
            + LV YRC PG   +++  IC ++Y++I C    ++E +V+  A L         P+I +   S G CP         +D T  ++  C     CSV P          S+C   HL L+YACVP  VLE+        ++ PSK + P + K +R
Sbjct:  145 EMLVAYRCEPGPQMVQSLAICQDTYVEIKCTTYGVEESIVVLDAQLVMQVDTKVCPKIVEPPSSAGVCPSR-------LDVTAGLMGMCGRERTCSVMPDASMLPPGYPSLCARRHLELSYACVPPSVLESAWKVMGKHLSRPSKPRQPYQKKNRR 293
[Close]
BLAST of SMED30023566 vs. TrEMBL
Match: A0A0S4MML8 (Uncharacterized protein OS=Echinococcus multilocularis OX=6211 PE=4 SV=1)

HSP 1 Score: 61.6178 bits (148), Expect = 3.308e-6
Identity = 48/156 (30.77%), Postives = 73/156 (46.79%), Query Frame = 1 
Query:  421 DFLVKYRCVPG--YIKNENICTNSYLDIDCFD-KLKEKLVIHKATLY--------PEIEKSEDSIG-CPLNSPKQCRPVDKTYHVISKCSYRDKCSVGPHEYT-----SSMCKNMHLRLTYACVPEDVLEN-------FMNEPSK-EKPIKTKTKR 813
            + LV YRC PG   +++  IC ++Y++I C    ++E +V+  A L         P+I +   S G CP         +D T  ++  C     CSV P          S+C   HL L+YACVP  VLE+        ++ PSK  +P + K +R
Sbjct:  145 EMLVAYRCEPGPQMVQSLAICQDTYVEIKCTTYGVEESIVVLDAQLVMQVDTKVCPKIVEPPSSAGVCPSR-------LDVTEGLMGMCGRERTCSVMPDASMLPLGYPSLCARRHLELSYACVPPSVLESAWKVMGKHLSRPSKPRQPYQKKNRR 293
[Close]
BLAST of SMED30023566 vs. Planmine SMEST
Match: SMESG000069363.1 (SMESG000069363.1)

HSP 1 Score: 843.573 bits (2178), Expect = 0.000e+0
Identity = 441/442 (99.77%), Postives = 441/442 (99.77%), Query Frame = 1 
Query:   61 MKIWIFIFLSLQNFQAYLSTSILMKDFKLFSAEICKIGCTTISCHSNTLISIDSITSIQASDNSKCSPINHSYSLSPYNCYFKYFENYILKTCANSFSCRICMNDIESVHTPNTQCKSEQDFLVKYRCVPGYIKNENICTNSYLDIDCFDKLKEKLVIHKATLYPEIEKSEDSIGCPLNSPKQCRPVDKTYHVISKCSYRDKCSVGPHEYTSSMCKNMHLRLTYACVPEDVLENFMNEPSKEKPIKTKTKRFEVENDTNSWFKKHKWTILGMXXXXXXXXXXXXXXXXCCNKRNQTNRSCDINTPKDNLFNKLGQQKFTPPPSSIDKVNVEFENATLAPPDIYNSNSDTEESNLRSMYPANIHSTMSYNSQFCNAPPLINTCQYFSPLVKVGHINMLEMPAIQLAXXXXXXXXXXXXXHGTPRGTKSSGIFSMTDMSFENTH 1386
            MKIWIFIFLSLQNFQAYLSTSILMKDFKLFSAEICKIGCTTISCHSNTLISIDSI SIQASDNSKCSPINHSYSLSPYNCYFKYFENYILKTCANSFSCRICMNDIESVHTPNTQCKSEQDFLVKYRCVPGYIKNENICTNSYLDIDCFDKLKEKLVIHKATLYPEIEKSEDSIGCPLNSPKQCRPVDKTYHVISKCSYRDKCSVGPHEYTSSMCKNMHLRLTYACVPEDVLENFMNEPSKEKPIKTKTKRFEVENDTNSWFKKHKWTILGMSVSIVVISSVLISVLICCNKRNQTNRSCDINTPKDNLFNKLGQQKFTPPPSSIDKVNVEFENATLAPPDIYNSNSDTEESNLRSMYPANIHSTMSYNSQFCNAPPLINTCQYFSPLVKVGHINMLEMPAIQLASSSNLTSSTINSSHGTPRGTKSSGIFSMTDMSFENTH
Sbjct:    1 MKIWIFIFLSLQNFQAYLSTSILMKDFKLFSAEICKIGCTTISCHSNTLISIDSINSIQASDNSKCSPINHSYSLSPYNCYFKYFENYILKTCANSFSCRICMNDIESVHTPNTQCKSEQDFLVKYRCVPGYIKNENICTNSYLDIDCFDKLKEKLVIHKATLYPEIEKSEDSIGCPLNSPKQCRPVDKTYHVISKCSYRDKCSVGPHEYTSSMCKNMHLRLTYACVPEDVLENFMNEPSKEKPIKTKTKRFEVENDTNSWFKKHKWTILGMSVSIVVISSVLISVLICCNKRNQTNRSCDINTPKDNLFNKLGQQKFTPPPSSIDKVNVEFENATLAPPDIYNSNSDTEESNLRSMYPANIHSTMSYNSQFCNAPPLINTCQYFSPLVKVGHINMLEMPAIQLASSSNLTSSTINSSHGTPRGTKSSGIFSMTDMSFENTH 442
[Close]
BLAST of SMED30023566 vs. Planmine SMEST
Match: SMESG000069363.1 (SMESG000069363.1)

HSP 1 Score: 796.964 bits (2057), Expect = 0.000e+0
Identity = 418/419 (99.76%), Postives = 418/419 (99.76%), Query Frame = 1 
Query:  130 MKDFKLFSAEICKIGCTTISCHSNTLISIDSITSIQASDNSKCSPINHSYSLSPYNCYFKYFENYILKTCANSFSCRICMNDIESVHTPNTQCKSEQDFLVKYRCVPGYIKNENICTNSYLDIDCFDKLKEKLVIHKATLYPEIEKSEDSIGCPLNSPKQCRPVDKTYHVISKCSYRDKCSVGPHEYTSSMCKNMHLRLTYACVPEDVLENFMNEPSKEKPIKTKTKRFEVENDTNSWFKKHKWTILGMXXXXXXXXXXXXXXXXCCNKRNQTNRSCDINTPKDNLFNKLGQQKFTPPPSSIDKVNVEFENATLAPPDIYNSNSDTEESNLRSMYPANIHSTMSYNSQFCNAPPLINTCQYFSPLVKVGHINMLEMPAIQLAXXXXXXXXXXXXXHGTPRGTKSSGIFSMTDMSFENTH 1386
            MKDFKLFSAEICKIGCTTISCHSNTLISIDSI SIQASDNSKCSPINHSYSLSPYNCYFKYFENYILKTCANSFSCRICMNDIESVHTPNTQCKSEQDFLVKYRCVPGYIKNENICTNSYLDIDCFDKLKEKLVIHKATLYPEIEKSEDSIGCPLNSPKQCRPVDKTYHVISKCSYRDKCSVGPHEYTSSMCKNMHLRLTYACVPEDVLENFMNEPSKEKPIKTKTKRFEVENDTNSWFKKHKWTILGMSVSIVVISSVLISVLICCNKRNQTNRSCDINTPKDNLFNKLGQQKFTPPPSSIDKVNVEFENATLAPPDIYNSNSDTEESNLRSMYPANIHSTMSYNSQFCNAPPLINTCQYFSPLVKVGHINMLEMPAIQLASSSNLTSSTINSSHGTPRGTKSSGIFSMTDMSFENTH
Sbjct:    1 MKDFKLFSAEICKIGCTTISCHSNTLISIDSINSIQASDNSKCSPINHSYSLSPYNCYFKYFENYILKTCANSFSCRICMNDIESVHTPNTQCKSEQDFLVKYRCVPGYIKNENICTNSYLDIDCFDKLKEKLVIHKATLYPEIEKSEDSIGCPLNSPKQCRPVDKTYHVISKCSYRDKCSVGPHEYTSSMCKNMHLRLTYACVPEDVLENFMNEPSKEKPIKTKTKRFEVENDTNSWFKKHKWTILGMSVSIVVISSVLISVLICCNKRNQTNRSCDINTPKDNLFNKLGQQKFTPPPSSIDKVNVEFENATLAPPDIYNSNSDTEESNLRSMYPANIHSTMSYNSQFCNAPPLINTCQYFSPLVKVGHINMLEMPAIQLASSSNLTSSTINSSHGTPRGTKSSGIFSMTDMSFENTH 419
[Close]
BLAST of SMED30023566 vs. Planmine SMEST
Match: SMESG000008954.1 (SMESG000008954.1)

HSP 1 Score: 96.2857 bits (238), Expect = 3.196e-21
Identity = 93/352 (26.42%), Postives = 159/352 (45.17%), Query Frame = 1 
Query:  121 SILMKDFKLFSAEICKIGCTTISCHSNTLISIDSIT-SIQASDNSKC-SPINHSYSL----SPYNCYFK---YFENYILKTCANSFSCRICMNDIESVHTPNTQCKSEQDFLVKYRCVPGYIKNENICTNSYLDIDCFDKLKEKLVIHKATLYPEIEKSEDSIGCPLNSPKQ--CRPVDKTYHVISKCSYRDKCSVGPHEYTSSMCKNMHLRLTYACVPEDVLENFMNEPSKEKPI---KTKTKRFE-------VENDTNSWFKKHK----------WTILGMXXXXXXXXXXXXXXXXCCNKRNQTNRSCDINTPKDNLFNKLGQQKFTPPPSSIDKVNVEFENATLAPPD 1083
            SI  + FK F+  IC   C  I C  NT++ I  +  S  A +   C SP + + SL     P NC  +   ++  +I   C+N   C++C  +     T    C    + +V Y C+   +  ENI  NSY ++ C   L  +++I+KA+L    +K+  ++    N  KQ  C  VD + ++   CS++ +C  GP++  S  C+   L L YACV E V++    E  + +P+    T   RF           + +++ KK K          W I G SV +V++            ++ +T ++        +LF+K+    F PPP+ I+K+ ++  +     PD
Sbjct:    5 SISQEMFKEFNKSICGRQCEKIYCPFNTVMKIQLVELSFGAKNEPNCMSPDSPNESLIELEDPDNCKARLEIHWRQFIHSECSNKTKCKVC--ETTETKTEQKGCYQIANIIVTYLCISNDVVEENITANSYYELTCEHLLSHRIIIYKASLALH-KKNPSNMKVFSNREKQKDCSQVDVSANISETCSFKRRCQAGPNQLFSGDCEKKQLLLQYACVHESVIK---KERQRNRPLFIHFTNPTRFHNNDIPILSSTEISTYLKKPKSDETIEDKLIWIITGSSVLVVMVMIFTAISWSYGMRKCRTRQT--------SLFHKIKDANFCPPPTPINKIQIDLPSRESIFPD 342
[Close]
BLAST of SMED30023566 vs. Planmine SMEST
Match: SMESG000008954.1 (SMESG000008954.1)

HSP 1 Score: 95.5153 bits (236), Expect = 7.293e-21
Identity = 91/346 (26.30%), Postives = 156/346 (45.09%), Query Frame = 1 
Query:  139 FKLFSAEICKIGCTTISCHSNTLISIDSI-TSIQASDNSKC-SPINHSYSL----SPYNCYFK---YFENYILKTCANSFSCRICMNDIESVHTPNTQCKSEQDFLVKYRCVPGYIKNENICTNSYLDIDCFDKLKEKLVIHKATLYPEIEKSEDSIGCPLNSPKQ--CRPVDKTYHVISKCSYRDKCSVGPHEYTSSMCKNMHLRLTYACVPEDVLENFMNEPSKEKPI---KTKTKRFE-------VENDTNSWFKKHK----------WTILGMXXXXXXXXXXXXXXXXCCNKRNQTNRSCDINTPKDNLFNKLGQQKFTPPPSSIDKVNVEFENATLAPPD 1083
            FK F+  IC   C  I C  NT++ I  +  S  A +   C SP + + SL     P NC  +   ++  +I   C+N   C++C  +     T    C    + +V Y C+   +  ENI  NSY ++ C   L  +++I+KA+L    +K+  ++    N  KQ  C  VD + ++   CS++ +C  GP++  S  C+   L L YACV E V++    E  + +P+    T   RF           + +++ KK K          W I G SV +V++            ++ +T ++        +LF+K+    F PPP+ I+K+ ++  +     PD
Sbjct:   23 FKEFNKSICGRQCEKIYCPFNTVMKIQLVELSFGAKNEPNCMSPDSPNESLIELEDPDNCKARLEIHWRQFIHSECSNKTKCKVC--ETTETKTEQKGCYQIANIIVTYLCISNDVVEENITANSYYELTCEHLLSHRIIIYKASLALH-KKNPSNMKVFSNREKQKDCSQVDVSANISETCSFKRRCQAGPNQLFSGDCEKKQLLLQYACVHESVIK---KERQRNRPLFIHFTNPTRFHNNDIPILSSTEISTYLKKPKSDETIEDKLIWIITGSSVLVVMVMIFTAISWSYGMRKCRTRQT--------SLFHKIKDANFCPPPTPINKIQIDLPSRESIFPD 354
[Close]
BLAST of SMED30023566 vs. Planmine SMEST
Match: SMESG000008954.1 (SMESG000008954.1)

HSP 1 Score: 95.5153 bits (236), Expect = 7.293e-21
Identity = 91/346 (26.30%), Postives = 156/346 (45.09%), Query Frame = 1 
Query:  139 FKLFSAEICKIGCTTISCHSNTLISIDSI-TSIQASDNSKC-SPINHSYSL----SPYNCYFK---YFENYILKTCANSFSCRICMNDIESVHTPNTQCKSEQDFLVKYRCVPGYIKNENICTNSYLDIDCFDKLKEKLVIHKATLYPEIEKSEDSIGCPLNSPKQ--CRPVDKTYHVISKCSYRDKCSVGPHEYTSSMCKNMHLRLTYACVPEDVLENFMNEPSKEKPI---KTKTKRFE-------VENDTNSWFKKHK----------WTILGMXXXXXXXXXXXXXXXXCCNKRNQTNRSCDINTPKDNLFNKLGQQKFTPPPSSIDKVNVEFENATLAPPD 1083
            FK F+  IC   C  I C  NT++ I  +  S  A +   C SP + + SL     P NC  +   ++  +I   C+N   C++C  +     T    C    + +V Y C+   +  ENI  NSY ++ C   L  +++I+KA+L    +K+  ++    N  KQ  C  VD + ++   CS++ +C  GP++  S  C+   L L YACV E V++    E  + +P+    T   RF           + +++ KK K          W I G SV +V++            ++ +T ++        +LF+K+    F PPP+ I+K+ ++  +     PD
Sbjct:   23 FKEFNKSICGRQCEKIYCPFNTVMKIQLVELSFGAKNEPNCMSPDSPNESLIELEDPDNCKARLEIHWRQFIHSECSNKTKCKVC--ETTETKTEQKGCYQIANIIVTYLCISNDVVEENITANSYYELTCEHLLSHRIIIYKASLALH-KKNPSNMKVFSNREKQKDCSQVDVSANISETCSFKRRCQAGPNQLFSGDCEKKQLLLQYACVHESVIK---KERQRNRPLFIHFTNPTRFHNNDIPILSSTEISTYLKKPKSDETIEDKLIWIITGSSVLVVMVMIFTAISWSYGMRKCRTRQT--------SLFHKIKDANFCPPPTPINKIQIDLPSRESIFPD 354
The following BLAST results are available for this feature:
BLAST of SMED30023566 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023566 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023566 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023566 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023566 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023566 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023566 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023566 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A0V0J7721.812e-727.50Protein eva-1 homolog C OS=Schistocephalus solidus... [more]
A0A0X3Q1T31.942e-727.50Protein eva-1 homolog C OS=Schistocephalus solidus... [more]
A0A0X3NXI22.099e-727.50Protein eva-1 homolog C OS=Schistocephalus solidus... [more]
A0A068WKG01.723e-630.77Pfam-B_8122 and DUF4381 domain containing protein ... [more]
A0A0S4MML83.308e-630.77Uncharacterized protein OS=Echinococcus multilocul... [more]
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BLAST of SMED30023566 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023566 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023566 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023566 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023566 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023566 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000069363.10.000e+099.77SMESG000069363.1[more]
SMESG000069363.10.000e+099.76SMESG000069363.1[more]
SMESG000008954.13.196e-2126.42SMESG000008954.1[more]
SMESG000008954.17.293e-2126.30SMESG000008954.1[more]
SMESG000008954.17.293e-2126.30SMESG000008954.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30023566 ID=SMED30023566|Name=SMED30023566|organism=Schmidtea mediterranea sexual|type=transcript|length=1541bp
TTTTTTTTGATGAGTTAACTTGAAAATTACTGTAGTCAAACGGAAAATTT
GTTTTTTACAATGAAAATTTGGATTTTTATTTTTTTATCTTTGCAAAATT
TCCAGGCGTATCTATCTACTAGTATTCTGATGAAAGATTTCAAGTTGTTC
TCGGCAGAAATCTGCAAAATCGGCTGTACCACCATCAGTTGCCATTCGAA
TACTCTGATATCCATAGATTCCATCACCTCAATCCAAGCCTCGGATAATT
CGAAATGCAGCCCAATTAATCATTCATATTCTCTGTCCCCTTATAATTGC
TATTTCAAATATTTTGAGAATTACATTTTGAAAACGTGTGCAAATAGTTT
CAGCTGTAGGATTTGCATGAATGACATCGAATCAGTGCATACCCCGAATA
CTCAGTGCAAATCTGAGCAGGACTTCCTAGTCAAATATCGCTGCGTTCCC
GGTTATATAAAAAACGAGAACATCTGTACAAATTCGTACCTTGACATTGA
CTGTTTCGATAAACTGAAAGAAAAATTAGTCATTCATAAAGCTACCCTTT
ATCCTGAAATTGAAAAATCAGAAGATTCCATCGGTTGTCCTCTCAATTCG
CCTAAACAGTGCCGCCCGGTGGACAAAACTTATCATGTTATTTCCAAGTG
CTCTTACCGTGACAAGTGCAGTGTTGGTCCGCACGAGTACACTTCGTCAA
TGTGCAAAAACATGCATTTGAGACTCACATATGCTTGTGTTCCAGAAGAT
GTGCTTGAAAATTTTATGAACGAACCTTCAAAGGAGAAACCGATAAAAAC
GAAAACCAAAAGATTCGAAGTCGAGAATGATACCAATTCATGGTTTAAAA
AGCACAAGTGGACAATTCTGGGAATGTCAGTGTCAATTGTTGTTATCTCT
TCAGTTTTGATCTCGGTTTTGATCTGTTGCAATAAAAGGAATCAGACGAA
TCGCAGCTGCGATATTAATACTCCAAAAGATAATTTATTTAATAAATTAG
GACAACAAAAGTTTACTCCTCCCCCTTCATCTATTGACAAAGTCAATGTT
GAATTCGAAAATGCAACCTTAGCCCCTCCAGATATCTACAACTCTAACTC
AGACACAGAGGAATCAAATCTTAGATCAATGTATCCTGCCAATATTCACA
GTACTATGTCGTATAATTCTCAATTCTGCAATGCTCCCCCTTTGATAAAT
ACTTGCCAGTATTTCTCTCCTCTGGTAAAGGTGGGACACATAAACATGCT
TGAAATGCCAGCAATCCAGCTTGCCAGTAGTAGCAATTTAACTTCTTCAA
CAATCAATTCATCCCATGGAACTCCAAGAGGAACCAAATCATCTGGCATC
TTTTCCATGACAGACATGTCTTTTGAAAATACTCATCTCGTTTAGTTATT
TTTGCAATTTGTTTTTTATTTTCCTTGTAATTCTGATGAATTGTGTGAAC
CTCAGCATTTATGGCTACCGTATTGGATTTGCTTTTTGATGAAACCGTAA
TTTTCATTGTACCAATTAAGCATTGCAAATTTTTAGTGAAC
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protein sequence of SMED30023566-orf-1

>SMED30023566-orf-1 ID=SMED30023566-orf-1|Name=SMED30023566-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=445bp
MKIWIFIFLSLQNFQAYLSTSILMKDFKLFSAEICKIGCTTISCHSNTLI
SIDSITSIQASDNSKCSPINHSYSLSPYNCYFKYFENYILKTCANSFSCR
ICMNDIESVHTPNTQCKSEQDFLVKYRCVPGYIKNENICTNSYLDIDCFD
KLKEKLVIHKATLYPEIEKSEDSIGCPLNSPKQCRPVDKTYHVISKCSYR
DKCSVGPHEYTSSMCKNMHLRLTYACVPEDVLENFMNEPSKEKPIKTKTK
RFEVENDTNSWFKKHKWTILGMSVSIVVISSVLISVLICCNKRNQTNRSC
DINTPKDNLFNKLGQQKFTPPPSSIDKVNVEFENATLAPPDIYNSNSDTE
ESNLRSMYPANIHSTMSYNSQFCNAPPLINTCQYFSPLVKVGHINMLEMP
AIQLASSSNLTSSTINSSHGTPRGTKSSGIFSMTDMSFENTHLV*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000101muscle cell
PLANA:0000142posterior region of the whole animal
PLANA:0000418head
PLANA:0002056medial muscle cell
Vocabulary: INTERPRO
TermDefinition
IPR019774Aromatic-AA_hydroxylase_C
IPR002108ADF-H
Vocabulary: biological process
TermDefinition
GO:0055114oxidation-reduction process
Vocabulary: molecular function
TermDefinition
GO:0016714oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced pteridine as one donor, and incorporation of one atom of oxygen
GO:0003779actin binding
Vocabulary: cellular component
TermDefinition
GO:0005622intracellular
GO:0016020membrane
GO:0016021integral component of membrane
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableSIGNALP_EUKSignalP-noTMSignalP-noTMcoord: 1..19
score: 0.585
NoneNo IPR availableTMHMMTMhelixcoord: 267..289