Autophagy-related protein 3

Overview
NameAutophagy-related protein 3
Smed IDSMED30023558
Length (bp)1191
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Autophagy-related protein 3 (SMED30023558) t-SNE clustered cells

Violin plots show distribution of expression levels for Autophagy-related protein 3 (SMED30023558) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Autophagy-related protein 3 (SMED30023558) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Autophagy-related protein 3 (SMED30023558) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30023558

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 9

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
intestinal phagocyteSMED30023558h1SMcG0017004 Contig4580GPL15192PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
Smed sexual biotypeSMED30023558h1SMcG0017004 Contig39059uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30023558h1SMcG0017004 Contig39059newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
nervous systemSMED30023558h1SMcG0017004 dd_Smed_v4_1915_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30023558h1SMcG0017004 dd_Smed_v4_1915_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30023558h1SMcG0017004 dd_Smed_v4_1915_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30023558h1SMcG0017004 dd_Smed_v4_1915_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30023558h1SMcG0017004 dd_Smed_v4_1915_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30023558h1SMcG0017004 dd_Smed_v4_1915_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Autophagy-related protein 3 vs. Ensembl Human
Match: ATG3 (autophagy related 3 [Source:HGNC Symbol;Acc:HGNC:20962])

HSP 1 Score: 321.627 bits (823), Expect = 5.231e-108
Identity = 179/329 (54.41%), Postives = 229/329 (69.60%), Query Frame = 3 
Query:  117 MHTMMQAMSRTALGVAEFLTPVLKESKFKETGVITPEEFVASGDFIVYHCPTWQWASGDPSKKKNYLPPDKQYLITRNLPCYKRVSHLFSNQNQNEKLVEPEDPDGGWVDAFHADESVSTITEKASEMILKDCKSSDERNFSPKHFNAGXXXXXXXXXXXAVDMAAFMKGECQFEDDDLIAPPTHSVIINK--EILKAQ-DFSQSD-IIQTRTYDLQITYDKLYQTCRLWLCGYDEDRSLLTEEQMFEDFSQDHVKKTVTMEVHPHLPGGVLMASVHPCRQAEVMKKLIEAVAEGGAVLGVHQYIVIFLKFVQAVIPTIEYDYTRNFNM 1091
            M  ++  +   AL VAE+LTPVLKESKFKETGVITPEEFVA+GD +V+HCPTWQWA+G+  K K YLP  KQ+L+T+N+PCYKR   +    ++ E ++E +D DGGWVD +H +  ++ ITE   E+ L+     ++ N   +  +A  ++++++D+ EA DM        ++E+  L+     ++   K  E  KA+ D    D I+QTRTYDL ITYDK YQT RLWL GYDE R  LT E M+ED SQDHVKKTVT+E HPHLP    M SVHPCR AEVMKK+IE VAEGG  LGVH Y++IFLKFVQAVIPTIEYDYTR+F M
Sbjct:    1 MQNVINTVKGKALEVAEYLTPVLKESKFKETGVITPEEFVAAGDHLVHHCPTWQWATGEELKVKAYLPTGKQFLVTKNVPCYKRCKQM-EYSDELEAIIEEDDGDGGWVDTYH-NTGITGITEAVKEITLE-----NKDNIRLQDCSALCEEEEDEDEGEAADME-------EYEESGLLETDEATLDTRKIVEACKAKTDAGGEDAILQTRTYDLYITYDKYYQTPRLWLFGYDEQRQPLTVEHMYEDISQDHVKKTVTIENHPHLPPPP-MCSVHPCRHAEVMKKIIETVAEGGGELGVHMYLLIFLKFVQAVIPTIEYDYTRHFTM 314
[Close]
BLAST of Autophagy-related protein 3 vs. Ensembl Human
Match: ATG3 (autophagy related 3 [Source:HGNC Symbol;Acc:HGNC:20962])

HSP 1 Score: 281.567 bits (719), Expect = 2.059e-92
Identity = 161/319 (50.47%), Postives = 213/319 (66.77%), Query Frame = 3 
Query:  117 MHTMMQAMSRTALGVAEFLTPVLKESKFKETGVITPEEFVASGDFIVYHCPTWQWASGDPSKKKNYLPPDKQYLITRNLPCYKRVSHLFSNQNQNEKLVEPEDPDGGWVDAFHADESVSTITEKASEMILKDCKSSDERNFSPKHFNAGXXXXXXXXXXXAVDMAAFMKGECQFEDDDLIAPPTHSVIINK--EILKAQ-DFSQSD-IIQTRTYDLQITYDKLYQTCRLWLCGYDEDRSLLTEEQMFEDFSQDHVKKTVTMEVHPHLPGGVLMASVHPCRQAEVMKKLIEAVAEGGAVLGVHQYIVIFLKFVQAVIPTI 1061
            M  ++  +   AL VAE+LTPVLKESKFKETGVITPEEFVA+GD +V+HCPTWQWA+G+  K K YLP  KQ+L+T+N+PCYKR   +    ++ E ++E +D DGGWVD +H +  ++ ITE   E+ L+     ++ N   +  +A  ++++++D+ EA DM        ++E+  L+     ++   K  E  KA+ D    D I+QTRTYDL ITYDK YQT RLWL GYDE R  LT E M+ED SQDHVKKTVT+E HPHLP    M SVHPCR AEVMKK+IE VAEGG  LGVH Y  ++++ V   + TI
Sbjct:    1 MQNVINTVKGKALEVAEYLTPVLKESKFKETGVITPEEFVAAGDHLVHHCPTWQWATGEELKVKAYLPTGKQFLVTKNVPCYKRCKQM-EYSDELEAIIEEDDGDGGWVDTYH-NTGITGITEAVKEITLE-----NKDNIRLQDCSALCEEEEDEDEGEAADME-------EYEESGLLETDEATLDTRKIVEACKAKTDAGGEDAILQTRTYDLYITYDKYYQTPRLWLFGYDEQRQPLTVEHMYEDISQDHVKKTVTIENHPHLPPPP-MCSVHPCRHAEVMKKIIETVAEGGGELGVHMYPSLYVRLVAKWLLTI 304
[Close]
BLAST of Autophagy-related protein 3 vs. Ensembl Human
Match: ATG3 (autophagy related 3 [Source:HGNC Symbol;Acc:HGNC:20962])

HSP 1 Score: 103.99 bits (258), Expect = 5.096e-27
Identity = 47/70 (67.14%), Postives = 56/70 (80.00%), Query Frame = 3 
Query:  117 MHTMMQAMSRTALGVAEFLTPVLKESKFKETGVITPEEFVASGDFIVYHCPTWQWASGDPSKKKNYLPPD 326
            M  ++  +   AL VAE+LTPVLKESKFKETGVITPEEFVA+GD +V+HCPTWQWA+G+  K K YLP D
Sbjct:    1 MQNVINTVKGKALEVAEYLTPVLKESKFKETGVITPEEFVAAGDHLVHHCPTWQWATGEELKVKAYLPTD 70
[Close]
BLAST of Autophagy-related protein 3 vs. Ensembl Celegans
Match: atg-3 (Autophagy-related protein 3 [Source:UniProtKB/TrEMBL;Acc:U4PRD2])

HSP 1 Score: 159.844 bits (403), Expect = 9.408e-46
Identity = 76/138 (55.07%), Postives = 99/138 (71.74%), Query Frame = 3 
Query:  678 IINKEILKAQDFSQSDIIQTRTYDLQITYDKLYQTCRLWLCGYDEDRSLLTEEQMFEDFSQDHVKKTVTMEVHPHLPGGVLMASVHPCRQAEVMKKLIEAVAEGGAVLGVHQYIVIFLKFVQAVIPTIEYDYTRNFNM 1091
            ++ K+ L A + +  ++ + RTYDL I YDK YQ  RL+L GYDE+R  LT EQ +EDFS DH  KT+T+E HP +   + M +VHPC+ AE+MK+LI   AE G VLGVH+Y+ +FLKFVQAVIPTIEYDYTR   +
Sbjct:  170 VVEKKPLAAGNDNSGEVEKVRTYDLHICYDKYYQVPRLFLMGYDENRRPLTVEQTYEDFSADHSNKTITVEAHPSV--DLTMPTVHPCKHAEMMKRLINQYAESGKVLGVHEYLFLFLKFVQAVIPTIEYDYTRAIKL 305

HSP 2 Score: 126.716 bits (317), Expect = 2.930e-33
Identity = 59/120 (49.17%), Postives = 84/120 (70.00%), Query Frame = 3 
Query:  117 MHTMMQAMSRTALGVAEFLTPVLKESKFKETGVITPEEFVASGDFIVYHCPTWQWA-SGDPSKKKNYLPPDKQYLITRNLPCYKRVSHLFSNQNQNEKLVEPEDP------DGGWVDAFH 455
            M  ++  +   AL + E  TPVL+ESKF+ETGV+TPEE+VA+GD +V+HCPTW+WA + DPSK + +LP DKQ+LITRN+PC+KR   +  ++ + EK++  ED       + GWVD  H
Sbjct:    1 MQNLVNNLKSAALQIGETFTPVLRESKFRETGVLTPEEYVAAGDHLVHHCPTWKWAGASDPSKIRTFLPIDKQFLITRNVPCHKRCKQMEYDE-KLEKIINEEDGEYQTSDETGWVDTHH 119
[Close]
BLAST of Autophagy-related protein 3 vs. Ensembl Celegans
Match: atg-3 (Autophagy-related protein 3 [Source:UniProtKB/TrEMBL;Acc:U4PRD2])

HSP 1 Score: 125.946 bits (315), Expect = 1.650e-35
Identity = 59/97 (60.82%), Postives = 73/97 (75.26%), Query Frame = 3 
Query:  801 GYDEDRSLLTEEQMFEDFSQDHVKKTVTMEVHPHLPGGVLMASVHPCRQAEVMKKLIEAVAEGGAVLGVHQYIVIFLKFVQAVIPTIEYDYTRNFNM 1091
            GYDE+R  LT EQ +EDFS DH  KT+T+E HP +   + M +VHPC+ AE+MK+LI   AE G VLGVH+Y+ +FLKFVQAVIPTIEYDYTR   +
Sbjct:    2 GYDENRRPLTVEQTYEDFSADHSNKTITVEAHPSV--DLTMPTVHPCKHAEMMKRLINQYAESGKVLGVHEYLFLFLKFVQAVIPTIEYDYTRAIKL 96
[Close]
BLAST of Autophagy-related protein 3 vs. Ensembl Fly
Match: Atg3 (gene:FBgn0036813 transcript:FBtr0075055)

HSP 1 Score: 319.701 bits (818), Expect = 2.228e-107
Identity = 174/341 (51.03%), Postives = 221/341 (64.81%), Query Frame = 3 
Query:  117 MHTMMQAMSRTALGVAEFLTPVLKESKFKETGVITPEEFVASGDFIVYHCPTWQWASGDPSKKKNYLPPDKQYLITRNLPCYKRVSHLFSNQNQNEKLVEPEDPDGGWVDAFH-ADESVSTITEKASEMILKDCK----SSDERNFSPKHFNAGXXXXXXXXXXXAVDMAAFMKGECQFEDDDLIAPPTHSVIINKEILKAQ-----------DFSQSDIIQTRTYDLQITYDKLYQTCRLWLCGYDEDRSLLTEEQMFEDFSQDHVKKTVTMEVHPHLPGGVLMASVHPCRQAEVMKKLIEAVAEGGAVLGVHQYIVIFLKFVQAVIPTIEYDYTRNFNM 1091
            M +++  +  TAL VAE+LTPVLKESKF+ETGV+TPEEFVA+GD +V+HCPTWQWA+GD +K K YLP DKQ+LITRN+PCY+R   +       E LVE E  DGGWV+     D+  + + +K  E+ +++ K    + D    +P    AG   +DEDDD+          G  +  D      P  +    K   +A+           + S   ++ TRTYDL I+YDK YQT RLW+ GYDE R  LT EQM+ED SQDH KKTVTME HPHLPG   MASVHPCR A++MKK+I+ V EGG  LGVH Y++IFLKFVQ VIPTIEYD+T+NFNM
Sbjct:    1 MQSVLNTVKGTALNVAEYLTPVLKESKFRETGVLTPEEFVAAGDHLVHHCPTWQWAAGDETKTKPYLPKDKQFLITRNVPCYRRCKQM--EYVGEETLVEEESGDGGWVETHQLNDDGTTQLEDKICELTMEETKEEMHTPDSDKSAP---GAGGQAEDEDDDEAIDMDDFEESGMLELVD------PAVATTTRKPEPEAKASPVAAASGDAEASGDSVLHTRTYDLHISYDKYYQTPRLWVVGYDEQRKPLTVEQMYEDVSQDHAKKTVTMESHPHLPGPN-MASVHPCRHADIMKKIIQTVEEGGGQLGVHLYLIIFLKFVQTVIPTIEYDFTQNFNM 329
[Close]
BLAST of Autophagy-related protein 3 vs. Ensembl Zebrafish
Match: atg3 (autophagy related 3 [Source:NCBI gene;Acc:336632])

HSP 1 Score: 343.969 bits (881), Expect = 5.093e-117
Identity = 184/325 (56.62%), Postives = 228/325 (70.15%), Query Frame = 3 
Query:  117 MHTMMQAMSRTALGVAEFLTPVLKESKFKETGVITPEEFVASGDFIVYHCPTWQWASGDPSKKKNYLPPDKQYLITRNLPCYKRVSHLFSNQNQNEKLVEPEDPDGGWVDAFHADESVSTITEKASEMILKDCKSSDERNFSPKHFNAGXXXXXXXXXXXAVDMAAFMKGECQFEDDDLIAPPTHSVIINKEILKAQDFSQSDIIQTRTYDLQITYDKLYQTCRLWLCGYDEDRSLLTEEQMFEDFSQDHVKKTVTMEVHPHLPGGVLMASVHPCRQAEVMKKLIEAVAEGGAVLGVHQYIVIFLKFVQAVIPTIEYDYTRNFNM 1091
            M  ++ ++  TALGVAEFLTPVLKESKFKETGVITPEEFVA+GD +V+HCPTW+WASG+ +K K YLP DKQ+L+TRN+PCYKR   +    ++ E ++E +D DGGWVD FH +  V+ +TE   E+ L +    D  N + K    G+  DD+DD++         +     E DD     +    ++K   KA+   +  I+QTRTYDL ITYDK YQT RLWL GYDEDR  LT +QM+ED SQDHVKKTVT+E HP+LP    M SVHPCR AEVMKK+IE VAEGG  LGVH Y++IFLKFVQAVIPTIEYDYTR+F M
Sbjct:    1 MQNVINSVKGTALGVAEFLTPVLKESKFKETGVITPEEFVAAGDHLVHHCPTWKWASGEEAKVKPYLPNDKQFLLTRNVPCYKRCKQM-EYSDELEAIIEEDDGDGGWVDTFH-NSGVTGVTEAVREISLDN---KDNMNMNVKTGACGNSGDDDDDEEGEAADMEEYEESGLLETDDATLDTSKMADLSK--TKAEAGGEDAILQTRTYDLYITYDKYYQTPRLWLFGYDEDRQPLTVDQMYEDISQDHVKKTVTIENHPNLPPPA-MCSVHPCRHAEVMKKIIETVAEGGGELGVHMYLLIFLKFVQAVIPTIEYDYTRHFTM 317
[Close]
BLAST of Autophagy-related protein 3 vs. Ensembl Xenopus
Match: rnf11.2 (ring finger protein 11.2 [Source:Xenbase;Acc:XB-GENE-5888819])

HSP 1 Score: 331.257 bits (848), Expect = 5.846e-112
Identity = 187/329 (56.84%), Postives = 228/329 (69.30%), Query Frame = 3 
Query:  117 MHTMMQAMSRTALGVAEFLTPVLKESKFKETGVITPEEFVASGDFIVYHCPTWQWASGDPSKKKNYLPPDKQYLITRNLPCYKRVSHLFSNQNQNEKLVEPEDPDGGWVDAFHADESVSTITEKASEMIL--KDCKSSDERNFSPKHFNAGXXXXXXXXXXXAVDMAAFMKGECQFEDDDLIAPPTHSVIINKEILKAQ-DFSQSD-IIQTRTYDLQITYDKLYQTCRLWLCGYDEDRSLLTEEQMFEDFSQDHVKKTVTMEVHPHLPGGVLMASVHPCRQAEVMKKLIEAVAEGGAVLGVHQYIVIFLKFVQAVIPTIEYDYTRNFNM 1091
            M +++  +   AL VAE+LTPVLKESKFKETGVITPEEF+A+GD +V+HCPTWQW++G+ SK K YLP DKQ+L+T+N+PCYKR   +    ++ E ++E +D DGGWVD FH   S++ +TE   E+ L  +DC  +          N   DDDD+DD+ EA DM  +   E    D+D     T  +   KE  K + D    D I+QTRTYDL ITYDK YQT RLWL GYDE R  LT E M+ED SQDHVKKTVT+E HPHLP    M SVHPCR AEVMKK+IE VAEGG  LGVH Y++IFLKFVQAVIPTIEYDYTR+F M
Sbjct:    1 MQSVINTVKGKALEVAEYLTPVLKESKFKETGVITPEEFLAAGDHLVHHCPTWQWSAGEESKIKPYLPNDKQFLMTKNVPCYKRCKQM-EYSDEQEAIIEEDDGDGGWVDTFH--HSLTGVTEAVKEITLETQDCGKTTS--------NIAVDDDDDDDEGEAADMEDYE--ESGLLDNDDATVDTSKI---KEACKPKADLGGEDAILQTRTYDLYITYDKYYQTPRLWLFGYDEQRRPLTVENMYEDISQDHVKKTVTIENHPHLPPPP-MCSVHPCRHAEVMKKIIETVAEGGGELGVHMYLLIFLKFVQAVIPTIEYDYTRHFTM 312
[Close]
BLAST of Autophagy-related protein 3 vs. Ensembl Xenopus
Match: rnf11.2 (ring finger protein 11.2 [Source:Xenbase;Acc:XB-GENE-5888819])

HSP 1 Score: 293.508 bits (750), Expect = 3.618e-97
Identity = 174/329 (52.89%), Postives = 215/329 (65.35%), Query Frame = 3 
Query:  117 MHTMMQAMSRTALGVAEFLTPVLKESKFKETGVITPEEFVASGDFIVYHCPTWQWASGDPSKKKNYLPPDKQYLITRNLPCYKRVSHLFSNQNQNEKLVEPEDPDGGWVDAFHADESVSTITEKASEMIL--KDCKSSDERNFSPKHFNAGXXXXXXXXXXXAVDMAAFMKGECQFEDDDLIAPPTHSVIINKEILKAQ-DFSQSD-IIQTRTYDLQITYDKLYQTCRLWLCGYDEDRSLLTEEQMFEDFSQDHVKKTVTMEVHPHLPGGVLMASVHPCRQAEVMKKLIEAVAEGGAVLGVHQYIVIFLKFVQAVIPTIEYDYTRNFNM 1091
            M +++  +   AL VAE+LTPVLK          T   F+A+GD +V+HCPTWQW++G+ SK K YLP DKQ+L+T+N+PCYKR   +    ++ E ++E +D DGGWVD FH   S++ +TE   E+ L  +DC  +          N   DDDD+DD+ EA DM  +   E    D+D     T  +   KE  K + D    D I+QTRTYDL ITYDK YQT RLWL GYDE R  LT E M+ED SQDHVKKTVT+E HPHLP    M SVHPCR AEVMKK+IE VAEGG  LGVH Y++IFLKFVQAVIPTIEYDYTR+F M
Sbjct:    1 MQSVINTVKGKALEVAEYLTPVLKV-------CTTLYCFLAAGDHLVHHCPTWQWSAGEESKIKPYLPNDKQFLMTKNVPCYKRCKQM-EYSDEQEAIIEEDDGDGGWVDTFH--HSLTGVTEAVKEITLETQDCGKTTS--------NIAVDDDDDDDEGEAADMEDYE--ESGLLDNDDATVDTSKI---KEACKPKADLGGEDAILQTRTYDLYITYDKYYQTPRLWLFGYDEQRRPLTVENMYEDISQDHVKKTVTIENHPHLPPPP-MCSVHPCRHAEVMKKIIETVAEGGGELGVHMYLLIFLKFVQAVIPTIEYDYTRHFTM 305
[Close]
BLAST of Autophagy-related protein 3 vs. Ensembl Xenopus
Match: rnf11.2 (ring finger protein 11.2 [Source:Xenbase;Acc:XB-GENE-5888819])

HSP 1 Score: 287.345 bits (734), Expect = 6.984e-95
Identity = 168/310 (54.19%), Postives = 206/310 (66.45%), Query Frame = 3 
Query:  117 MHTMMQAMSRTALGVAEFLTPVLKESKFKETGVITPEEFVASGDFIVYHCPTWQWASGDPSKKKNYLPPDKQYLITRNLPCYKRVSHLFSNQNQNEKLVEPEDPDGGWVDAFHADESVSTITEKASEMIL--KDCKSSDERNFSPKHFNAGXXXXXXXXXXXAVDMAAFMKGECQFEDDDLIAPPTHSVIINKEILKAQ-DFSQSD-IIQTRTYDLQITYDKLYQTCRLWLCGYDEDRSLLTEEQMFEDFSQDHVKKTVTMEVHPHLPGGVLMASVHPCRQAEVMKKLIEAVAEGGAVLGVHQYIVIFLK 1034
            M +++  +   AL VAE+LTPVLKESKFKETGVITPEEF+A+GD +V+HCPTWQW++G+ SK K YLP DKQ+L+T+N+PCYKR   +  +  Q E ++E +D DGGWVD FH   S++ +TE   E+ L  +DC  +          N   DDDD+DD+ EA DM  +   E    D+D     T  +   KE  K + D    D I+QTRTYDL ITYDK YQT RLWL GYDE R  LT E M+ED SQDHVKKTVT+E HPHLP    M SVHPCR AEVMKK+IE VAEGG  LGVH Y  +  K
Sbjct:    1 MQSVINTVKGKALEVAEYLTPVLKESKFKETGVITPEEFLAAGDHLVHHCPTWQWSAGEESKIKPYLPNDKQFLMTKNVPCYKRCKQMEYSDEQ-EAIIEEDDGDGGWVDTFH--HSLTGVTEAVKEITLETQDCGKTTS--------NIAVDDDDDDDEGEAADMEDYE--ESGLLDNDDATVDTSKI---KEACKPKADLGGEDAILQTRTYDLYITYDKYYQTPRLWLFGYDEQRRPLTVENMYEDISQDHVKKTVTIENHPHLPPPP-MCSVHPCRHAEVMKKIIETVAEGGGELGVHMYPFLITK 293
[Close]
BLAST of Autophagy-related protein 3 vs. Ensembl Mouse
Match: Atg3 (autophagy related 3 [Source:MGI Symbol;Acc:MGI:1915091])

HSP 1 Score: 324.709 bits (831), Expect = 1.843e-109
Identity = 175/325 (53.85%), Postives = 219/325 (67.38%), Query Frame = 3 
Query:  117 MHTMMQAMSRTALGVAEFLTPVLKESKFKETGVITPEEFVASGDFIVYHCPTWQWASGDPSKKKNYLPPDKQYLITRNLPCYKRVSHLFSNQNQNEKLVEPEDPDGGWVDAFHADESVSTITEKASEMILKDCKSSDERNFSPKHFNAGXXXXXXXXXXXAVDMAAFMKGECQFEDDDLIAPPTHSVIINKEILKAQDFSQSDIIQTRTYDLQITYDKLYQTCRLWLCGYDEDRSLLTEEQMFEDFSQDHVKKTVTMEVHPHLPGGVLMASVHPCRQAEVMKKLIEAVAEGGAVLGVHQYIVIFLKFVQAVIPTIEYDYTRNFNM 1091
            M  ++  +   AL VAE+LTPVLKESKFKETGVITPEEFVA+GD +V+HCPTWQWA+G+  K K YLP DKQ+L+T+N+PCYKR   +    ++ E ++E +D DGGWVD +H +  ++ ITE   E+ L+   S   ++ S       ++D+ E  D E  + +  ++ +    D   I     +        KA    +  I+QTRTYDL ITYDK YQT RLWL GYDE R  LT E M+ED SQDHVKKTVT+E HPHLP    M SVHPCR AEVMKK+IE VAEGG  LGVH Y++IFLKFVQAVIPTIEYDYTR+F M
Sbjct:    1 MQNVINTVKGKALEVAEYLTPVLKESKFKETGVITPEEFVAAGDHLVHHCPTWQWATGEELKVKAYLPTDKQFLVTKNVPCYKRCKQM-EYSDELEAIIEEDDGDGGWVDTYH-NTGITGITEAVKEITLESKDSIKLQDCSALCDEEDEEDEGEAADMEEYEESGLLETDEATLDTRKIVEACKA--------KADAGGEDAILQTRTYDLYITYDKYYQTPRLWLFGYDEQRQPLTVEHMYEDISQDHVKKTVTIENHPHLPPPP-MCSVHPCRHAEVMKKIIETVAEGGGELGVHMYLLIFLKFVQAVIPTIEYDYTRHFTM 314
[Close]
BLAST of Autophagy-related protein 3 vs. UniProt/SwissProt
Match: sp|Q6PFS7|ATG3_DANRE (Ubiquitin-like-conjugating enzyme ATG3 OS=Danio rerio OX=7955 GN=atg3 PE=2 SV=1)

HSP 1 Score: 343.969 bits (881), Expect = 3.845e-116
Identity = 184/325 (56.62%), Postives = 228/325 (70.15%), Query Frame = 3 
Query:  117 MHTMMQAMSRTALGVAEFLTPVLKESKFKETGVITPEEFVASGDFIVYHCPTWQWASGDPSKKKNYLPPDKQYLITRNLPCYKRVSHLFSNQNQNEKLVEPEDPDGGWVDAFHADESVSTITEKASEMILKDCKSSDERNFSPKHFNAGXXXXXXXXXXXAVDMAAFMKGECQFEDDDLIAPPTHSVIINKEILKAQDFSQSDIIQTRTYDLQITYDKLYQTCRLWLCGYDEDRSLLTEEQMFEDFSQDHVKKTVTMEVHPHLPGGVLMASVHPCRQAEVMKKLIEAVAEGGAVLGVHQYIVIFLKFVQAVIPTIEYDYTRNFNM 1091
            M  ++ ++  TALGVAEFLTPVLKESKFKETGVITPEEFVA+GD +V+HCPTW+WASG+ +K K YLP DKQ+L+TRN+PCYKR   +    ++ E ++E +D DGGWVD FH +  V+ +TE   E+ L +    D  N + K    G+  DD+DD++         +     E DD     +    ++K   KA+   +  I+QTRTYDL ITYDK YQT RLWL GYDEDR  LT +QM+ED SQDHVKKTVT+E HP+LP    M SVHPCR AEVMKK+IE VAEGG  LGVH Y++IFLKFVQAVIPTIEYDYTR+F M
Sbjct:    1 MQNVINSVKGTALGVAEFLTPVLKESKFKETGVITPEEFVAAGDHLVHHCPTWKWASGEEAKVKPYLPNDKQFLLTRNVPCYKRCKQM-EYSDELEAIIEEDDGDGGWVDTFH-NSGVTGVTEAVREISLDN---KDNMNMNVKTGACGNSGDDDDDEEGEAADMEEYEESGLLETDDATLDTSKMADLSK--TKAEAGGEDAILQTRTYDLYITYDKYYQTPRLWLFGYDEDRQPLTVDQMYEDISQDHVKKTVTIENHPNLPPPA-MCSVHPCRHAEVMKKIIETVAEGGGELGVHMYLLIFLKFVQAVIPTIEYDYTRHFTM 317
[Close]
BLAST of Autophagy-related protein 3 vs. UniProt/SwissProt
Match: sp|Q5I0S6|ATG3_XENTR (Ubiquitin-like-conjugating enzyme ATG3 OS=Xenopus tropicalis OX=8364 GN=atg3 PE=2 SV=1)

HSP 1 Score: 331.257 bits (848), Expect = 3.097e-111
Identity = 187/329 (56.84%), Postives = 228/329 (69.30%), Query Frame = 3 
Query:  117 MHTMMQAMSRTALGVAEFLTPVLKESKFKETGVITPEEFVASGDFIVYHCPTWQWASGDPSKKKNYLPPDKQYLITRNLPCYKRVSHLFSNQNQNEKLVEPEDPDGGWVDAFHADESVSTITEKASEMIL--KDCKSSDERNFSPKHFNAGXXXXXXXXXXXAVDMAAFMKGECQFEDDDLIAPPTHSVIINKEILKAQ-DFSQSD-IIQTRTYDLQITYDKLYQTCRLWLCGYDEDRSLLTEEQMFEDFSQDHVKKTVTMEVHPHLPGGVLMASVHPCRQAEVMKKLIEAVAEGGAVLGVHQYIVIFLKFVQAVIPTIEYDYTRNFNM 1091
            M +++  +   AL VAE+LTPVLKESKFKETGVITPEEF+A+GD +V+HCPTWQW++G+ SK K YLP DKQ+L+T+N+PCYKR   +    ++ E ++E +D DGGWVD FH   S++ +TE   E+ L  +DC  +          N   DDDD+DD+ EA DM  +   E    D+D     T  +   KE  K + D    D I+QTRTYDL ITYDK YQT RLWL GYDE R  LT E M+ED SQDHVKKTVT+E HPHLP    M SVHPCR AEVMKK+IE VAEGG  LGVH Y++IFLKFVQAVIPTIEYDYTR+F M
Sbjct:    1 MQSVINTVKGKALEVAEYLTPVLKESKFKETGVITPEEFLAAGDHLVHHCPTWQWSAGEESKIKPYLPNDKQFLMTKNVPCYKRCKQM-EYSDEQEAIIEEDDGDGGWVDTFH--HSLTGVTEAVKEITLETQDCGKTTS--------NIAVDDDDDDDEGEAADMEDYE--ESGLLDNDDATVDTSKI---KEACKPKADLGGEDAILQTRTYDLYITYDKYYQTPRLWLFGYDEQRRPLTVENMYEDISQDHVKKTVTIENHPHLPPPP-MCSVHPCRHAEVMKKIIETVAEGGGELGVHMYLLIFLKFVQAVIPTIEYDYTRHFTM 312
[Close]
BLAST of Autophagy-related protein 3 vs. UniProt/SwissProt
Match: sp|Q6GQE7|ATG3_XENLA (Ubiquitin-like-conjugating enzyme ATG3 OS=Xenopus laevis OX=8355 GN=atg3 PE=2 SV=1)

HSP 1 Score: 327.791 bits (839), Expect = 7.361e-110
Identity = 186/328 (56.71%), Postives = 225/328 (68.60%), Query Frame = 3 
Query:  117 MHTMMQAMSRTALGVAEFLTPVLKESKFKETGVITPEEFVASGDFIVYHCPTWQWASGDPSKKKNYLPPDKQYLITRNLPCYKRVSHLFSNQNQNEKLVEPEDPDGGWVDAFHADESVSTITEKASEMIL--KDC-KSSDERNFSPKHFNAGXXXXXXXXXXXAVDMAAFMKGECQFEDDDLIAPPTHSVIINKEILKAQDFSQSDIIQTRTYDLQITYDKLYQTCRLWLCGYDEDRSLLTEEQMFEDFSQDHVKKTVTMEVHPHLPGGVLMASVHPCRQAEVMKKLIEAVAEGGAVLGVHQYIVIFLKFVQAVIPTIEYDYTRNFNM 1091
            M  +   +   AL VAE+LTPVLKESKFKETGVITPEEF+A+GD +V+HCPTWQW++G+ SK K YLP DKQ+L+T+N+PCYKR   +    ++ E ++E +D DGGWVD FH    +S +TE   E+ L  +DC K++D         N    DDD+DD+ EA DM  + +     E+DD  A    S I      KA    +  I+QTRTYDL ITYDK YQT RLWL GYDE R  L  E M+ED SQDHVKKTVT+E HPHLP    M SVHPCR AEVMKK+IE VAEGG  LGVH Y++IFLKFVQAVIPTIEYDYTR+F M
Sbjct:    1 MQNVFNTVKGKALEVAEYLTPVLKESKFKETGVITPEEFLAAGDHLVHHCPTWQWSAGEESKIKPYLPNDKQFLMTKNVPCYKRCKQM-EYSDEQEAIIEEDDGDGGWVDTFHHT-GLSGVTEAVKEITLETQDCGKTTD---------NIAVCDDDDDDEGEAADMEDYEESGL-LENDD--ATVDTSKIKEACKPKADLGGEDAILQTRTYDLYITYDKYYQTPRLWLFGYDEQRRPLAVENMYEDISQDHVKKTVTIENHPHLPPPP-MCSVHPCRHAEVMKKIIETVAEGGGELGVHMYLLIFLKFVQAVIPTIEYDYTRHFTM 313
[Close]
BLAST of Autophagy-related protein 3 vs. UniProt/SwissProt
Match: sp|Q9CPX6|ATG3_MOUSE (Ubiquitin-like-conjugating enzyme ATG3 OS=Mus musculus OX=10090 GN=Atg3 PE=1 SV=1)

HSP 1 Score: 324.709 bits (831), Expect = 1.289e-108
Identity = 175/325 (53.85%), Postives = 219/325 (67.38%), Query Frame = 3 
Query:  117 MHTMMQAMSRTALGVAEFLTPVLKESKFKETGVITPEEFVASGDFIVYHCPTWQWASGDPSKKKNYLPPDKQYLITRNLPCYKRVSHLFSNQNQNEKLVEPEDPDGGWVDAFHADESVSTITEKASEMILKDCKSSDERNFSPKHFNAGXXXXXXXXXXXAVDMAAFMKGECQFEDDDLIAPPTHSVIINKEILKAQDFSQSDIIQTRTYDLQITYDKLYQTCRLWLCGYDEDRSLLTEEQMFEDFSQDHVKKTVTMEVHPHLPGGVLMASVHPCRQAEVMKKLIEAVAEGGAVLGVHQYIVIFLKFVQAVIPTIEYDYTRNFNM 1091
            M  ++  +   AL VAE+LTPVLKESKFKETGVITPEEFVA+GD +V+HCPTWQWA+G+  K K YLP DKQ+L+T+N+PCYKR   +    ++ E ++E +D DGGWVD +H +  ++ ITE   E+ L+   S   ++ S       ++D+ E  D E  + +  ++ +    D   I     +        KA    +  I+QTRTYDL ITYDK YQT RLWL GYDE R  LT E M+ED SQDHVKKTVT+E HPHLP    M SVHPCR AEVMKK+IE VAEGG  LGVH Y++IFLKFVQAVIPTIEYDYTR+F M
Sbjct:    1 MQNVINTVKGKALEVAEYLTPVLKESKFKETGVITPEEFVAAGDHLVHHCPTWQWATGEELKVKAYLPTDKQFLVTKNVPCYKRCKQM-EYSDELEAIIEEDDGDGGWVDTYH-NTGITGITEAVKEITLESKDSIKLQDCSALCDEEDEEDEGEAADMEEYEESGLLETDEATLDTRKIVEACKA--------KADAGGEDAILQTRTYDLYITYDKYYQTPRLWLFGYDEQRQPLTVEHMYEDISQDHVKKTVTIENHPHLPPPP-MCSVHPCRHAEVMKKIIETVAEGGGELGVHMYLLIFLKFVQAVIPTIEYDYTRHFTM 314
[Close]
BLAST of Autophagy-related protein 3 vs. UniProt/SwissProt
Match: sp|Q9NT62|ATG3_HUMAN (Ubiquitin-like-conjugating enzyme ATG3 OS=Homo sapiens OX=9606 GN=ATG3 PE=1 SV=1)

HSP 1 Score: 321.627 bits (823), Expect = 2.512e-107
Identity = 179/329 (54.41%), Postives = 229/329 (69.60%), Query Frame = 3 
Query:  117 MHTMMQAMSRTALGVAEFLTPVLKESKFKETGVITPEEFVASGDFIVYHCPTWQWASGDPSKKKNYLPPDKQYLITRNLPCYKRVSHLFSNQNQNEKLVEPEDPDGGWVDAFHADESVSTITEKASEMILKDCKSSDERNFSPKHFNAGXXXXXXXXXXXAVDMAAFMKGECQFEDDDLIAPPTHSVIINK--EILKAQ-DFSQSD-IIQTRTYDLQITYDKLYQTCRLWLCGYDEDRSLLTEEQMFEDFSQDHVKKTVTMEVHPHLPGGVLMASVHPCRQAEVMKKLIEAVAEGGAVLGVHQYIVIFLKFVQAVIPTIEYDYTRNFNM 1091
            M  ++  +   AL VAE+LTPVLKESKFKETGVITPEEFVA+GD +V+HCPTWQWA+G+  K K YLP  KQ+L+T+N+PCYKR   +    ++ E ++E +D DGGWVD +H +  ++ ITE   E+ L+     ++ N   +  +A  ++++++D+ EA DM        ++E+  L+     ++   K  E  KA+ D    D I+QTRTYDL ITYDK YQT RLWL GYDE R  LT E M+ED SQDHVKKTVT+E HPHLP    M SVHPCR AEVMKK+IE VAEGG  LGVH Y++IFLKFVQAVIPTIEYDYTR+F M
Sbjct:    1 MQNVINTVKGKALEVAEYLTPVLKESKFKETGVITPEEFVAAGDHLVHHCPTWQWATGEELKVKAYLPTGKQFLVTKNVPCYKRCKQM-EYSDELEAIIEEDDGDGGWVDTYH-NTGITGITEAVKEITLE-----NKDNIRLQDCSALCEEEEDEDEGEAADME-------EYEESGLLETDEATLDTRKIVEACKAKTDAGGEDAILQTRTYDLYITYDKYYQTPRLWLFGYDEQRQPLTVEHMYEDISQDHVKKTVTIENHPHLPPPP-MCSVHPCRHAEVMKKIIETVAEGGGELGVHMYLLIFLKFVQAVIPTIEYDYTRHFTM 314
[Close]
BLAST of Autophagy-related protein 3 vs. TrEMBL
Match: A0A220XHQ4 (Autophagy-related protein 3 OS=Dugesia japonica OX=6161 GN=Atg3 PE=2 SV=1)

HSP 1 Score: 588.956 bits (1517), Expect = 0.000e+0
Identity = 289/325 (88.92%), Postives = 303/325 (93.23%), Query Frame = 3 
Query:  117 MHTMMQAMSRTALGVAEFLTPVLKESKFKETGVITPEEFVASGDFIVYHCPTWQWASGDPSKKKNYLPPDKQYLITRNLPCYKRVSHLFSNQNQNEKLVEPEDPDGGWVDAFHADESVSTITEKASEMILKDCKSSDERNFSPKHFNAGXXXXXXXXXXXAVDMAAFMKGECQFEDDDLIAPPTHSVIINKEILKAQDFSQSDIIQTRTYDLQITYDKLYQTCRLWLCGYDEDRSLLTEEQMFEDFSQDHVKKTVTMEVHPHLPGGVLMASVHPCRQAEVMKKLIEAVAEGGAVLGVHQYIVIFLKFVQAVIPTIEYDYTRNFNM 1091
            MHTMMQAMSRTALGVAEFLTPVLKESKFKETGVITPEEFVASGDFIVYHCPTWQWASGD SK+K++LPPDKQYLITRNLPCYKRVSHLFSNQNQ EKLVEPEDPDGGWVDAFHADE+V+T+TEKASEM LK+ K+ D+RN   K+ N    DDD+D+DDEAVDMAAFMKGECQ EDDDLIAPP  S   NKEI+KA D  QSDI+QTRTYDLQITYDKLYQTCRLWLCGYDEDRSLLTEEQMFEDFSQDHVKKTVTMEVHPHLPGGVLMASVHPCRQAEVMKKLIEAVAEGGAVLGVHQYIVIFLKFVQAVIPTIEYDYTRNFNM
Sbjct:    1 MHTMMQAMSRTALGVAEFLTPVLKESKFKETGVITPEEFVASGDFIVYHCPTWQWASGDSSKRKSFLPPDKQYLITRNLPCYKRVSHLFSNQNQIEKLVEPEDPDGGWVDAFHADETVTTLTEKASEMTLKEYKNLDDRNIHSKNIN---FDDDDDEDDEAVDMAAFMKGECQLEDDDLIAPPNQSAKTNKEIIKATDACQSDIVQTRTYDLQITYDKLYQTCRLWLCGYDEDRSLLTEEQMFEDFSQDHVKKTVTMEVHPHLPGGVLMASVHPCRQAEVMKKLIEAVAEGGAVLGVHQYIVIFLKFVQAVIPTIEYDYTRNFNM 322
[Close]
BLAST of Autophagy-related protein 3 vs. TrEMBL
Match: K1R934 (Autophagy-related protein 3 OS=Crassostrea gigas OX=29159 GN=CGI_10024759 PE=3 SV=1)

HSP 1 Score: 363.999 bits (933), Expect = 1.764e-121
Identity = 199/332 (59.94%), Postives = 242/332 (72.89%), Query Frame = 3 
Query:  117 MHTMMQAMSRTALGVAEFLTPVLKESKFKETGVITPEEFVASGDFIVYHCPTWQWASGDPSKKKNYLPPDKQYLITRNLPCYKRVSHLFSNQNQNEKLVEPEDPDGGWVDAFH-ADESVSTITEKASEMILKDCKSSDERNFSPKHFNAG--XXXXXXXXXXXAVDMAAFMKGECQFEDDDLIAPPTHSVIINKEILKAQD----FSQSDIIQTRTYDLQITYDKLYQTCRLWLCGYDEDRSLLTEEQMFEDFSQDHVKKTVTMEVHPHLPGGVLMASVHPCRQAEVMKKLIEAVAEGGAVLGVHQYIVIFLKFVQAVIPTIEYDYTRNFNM 1091
            M  ++ A+  TAL VAE  TPVLKESKFKETGVITPEEFVA+GD +V+HCPTWQW++GD SK K YLP +KQYLITRN+PCYKRV  + S++ + EK++E +D DGGWVD  H  D   +++ +   EM L D K  D ++ S +   +G  DDDDD+DDD+EA+DM AF +     E+DD    P+         L  QD     ++S I+QTRTYDL ITYDK YQT RLWL GYDE+R  LT EQM+EDFSQDH KKTVTME HPHLPG  L ASVHPCR A+VMKK+I+ VAEGG  LGVH Y++IFLKFVQAVIPTIEYD+TR+F M
Sbjct:    1 MQNVINAVKGTALNVAEKFTPVLKESKFKETGVITPEEFVAAGDHLVHHCPTWQWSTGDESKVKPYLPKEKQYLITRNVPCYKRVKQVDSHKEEQEKVIEADDEDGGWVDTHHFPDPGTTSLQDAVQEMTL-DGKGKDRKSSSEEVKRSGDDDDDDDDDDDEEAMDMEAFEQSGMLDEEDDAALDPS--------TLGRQDSESAATESGILQTRTYDLNITYDKYYQTPRLWLFGYDENRKPLTVEQMYEDFSQDHAKKTVTMEAHPHLPGPPL-ASVHPCRHADVMKKIIQMVAEGGGDLGVHVYLMIFLKFVQAVIPTIEYDFTRDFTM 322
[Close]
BLAST of Autophagy-related protein 3 vs. TrEMBL
Match: A0A1S3JZA0 (Autophagy-related protein 3 OS=Lingula unguis OX=7574 GN=LOC106177197 PE=3 SV=1)

HSP 1 Score: 363.229 bits (931), Expect = 4.366e-121
Identity = 196/328 (59.76%), Postives = 239/328 (72.87%), Query Frame = 3 
Query:  117 MHTMMQAMSRTALGVAEFLTPVLKESKFKETGVITPEEFVASGDFIVYHCPTWQWASGDPSKKKNYLPPDKQYLITRNLPCYKRVSHLFSNQNQNEKLVEPEDPDGGWVDAFH-ADESVSTITEKASEMILKDCKSSDERNFSPKHFNAGXXXXXXXXXXXAVDMAAFMK-GECQFEDDDLIAPPTHSVIINKEILKAQ-DFSQSDIIQTRTYDLQITYDKLYQTCRLWLCGYDEDRSLLTEEQMFEDFSQDHVKKTVTMEVHPHLPGGVLMASVHPCRQAEVMKKLIEAVAEGGAVLGVHQYIVIFLKFVQAVIPTIEYDYTRNFNM 1091
            M  ++ A+  +ALGVAE+ TPVLKESKFKETGVITPEEFVA+GD IV+HCPTWQWASGD SK K YLP +KQ+LIT+N+PCY+RV  + ++Q ++E ++E +D DGGWVD  H AD SV  + +  +EM L     +     S      GDDD+D+ DD+EA DM AF + G  + ED   + P T    + +E   A     +S I+QTRTYDL ITYDK YQT RLWL GYDE+R  LT +QM+EDFSQDH KKTVTME HPHLP G  MASVHPCR AEVMKK+I+ VAEGG  LGVH Y++IFLKFVQAVIP+IEYDYTR+F M
Sbjct:    1 MQNVINAVKGSALGVAEYFTPVLKESKFKETGVITPEEFVAAGDHIVHHCPTWQWASGDESKIKPYLPREKQFLITKNVPCYRRVKQMDTHQEEHELVIETQDGDGGWVDTHHFADTSVGQVQDAVAEMTLDSKGGASATASSGAQQGGGDDDEDDGDDEEAEDMEAFEESGMLEAEDTATLDPAT----VQQETEAASAGAGESGILQTRTYDLNITYDKYYQTPRLWLYGYDENRKPLTVDQMYEDFSQDHAKKTVTMETHPHLP-GPPMASVHPCRHAEVMKKIIQTVAEGGGELGVHMYLMIFLKFVQAVIPSIEYDYTRHFTM 323
[Close]
BLAST of Autophagy-related protein 3 vs. TrEMBL
Match: A0A210QTW8 (Autophagy-related protein 3 OS=Mizuhopecten yessoensis OX=6573 GN=KP79_PYT20085 PE=3 SV=1)

HSP 1 Score: 352.443 bits (903), Expect = 6.437e-117
Identity = 201/329 (61.09%), Postives = 243/329 (73.86%), Query Frame = 3 
Query:  117 MHTMMQAMSRTALGVAEFLTPVLKESKFKETGVITPEEFVASGDFIVYHCPTWQWASGDPSKKKNYLPPDKQYLITRNLPCYKRVSHLFSNQNQNEKLVEPEDPDGGWVDAFH---ADESVSTITEKASEMILKDCKSSDERNFSPKHFNAGXXXXXXXXXXXAVDMAAFMK-GECQFEDDDLIAPPTHSVIINKEILKAQDFSQSDIIQTRTYDLQITYDKLYQTCRLWLCGYDEDRSLLTEEQMFEDFSQDHVKKTVTMEVHPHLPGGVLMASVHPCRQAEVMKKLIEAVAEGGAVLGVHQYIVIFLKFVQAVIPTIEYDYTRNFNM 1091
            M  M+ A+  TALG+AE+LTPVLKESKFKETGVITPEEF+A+GD IV+HCPTWQW++GD SK K YLP DKQ+LITRN+PCYKRV  + ++Q + EK++E ED DGGWVD  H   +  + ++I E  SEM L   +S    +  PK     DDDDDEDDD+EA+DM AF + G  + +D+  + P T+     ++        +S IIQTRTYDL ITYDK YQT RLWL GYDE+R  LT  QM+EDFSQDH+KKTVTME HPHLPG   MASVHPCR A VMKK+I+ VAEGG  LGVH Y++IFLKFVQAVIPTIEYDYTR+F M
Sbjct:    1 MQNMINAVKGTALGMAEYLTPVLKESKFKETGVITPEEFLAAGDHIVHHCPTWQWSAGDESKTKPYLPKDKQFLITRNVPCYKRVKQVDNHQEEQEKVIE-EDEDGGWVDTHHFPGSGVNATSIQEAVSEMTLDSKESKGSSSSQPK--GDDDDDDDEDDDEEAMDMEAFEESGMLEDKDNATLNPSTYE----RQESDGAMGGESGIIQTRTYDLNITYDKYYQTPRLWLYGYDENRKPLTVNQMYEDFSQDHIKKTVTMEAHPHLPGPP-MASVHPCRHAVVMKKIIQMVAEGGGDLGVHVYLMIFLKFVQAVIPTIEYDYTRHFIM 321
[Close]
BLAST of Autophagy-related protein 3 vs. TrEMBL
Match: A0A3Q8C0P2 (Autophagy-related protein 3 OS=Locusta migratoria OX=7004 GN=ATG3 PE=2 SV=1)

HSP 1 Score: 352.443 bits (903), Expect = 1.091e-116
Identity = 190/342 (55.56%), Postives = 236/342 (69.01%), Query Frame = 3 
Query:  117 MHTMMQAMSRTALGVAEFLTPVLKESKFKETGVITPEEFVASGDFIVYHCPTWQWASGDPSKKKNYLPPDKQYLITRNLPCYKRVSHLFSNQNQNEKLVEPEDPDGGWVDAFHADESVSTITEKASEMILK--------DCKSSDER---NFSPKHFNAGXX------XXXXXXXXXAVDMAAFMKGECQFEDDDLIAPPTHSVIINKEILKAQDFSQSDIIQTRTYDLQITYDKLYQTCRLWLCGYDEDRSLLTEEQMFEDFSQDHVKKTVTMEVHPHLPGGVLMASVHPCRQAEVMKKLIEAVAEGGAVLGVHQYIVIFLKFVQAVIPTIEYDYTRNFNM 1091
            M  ++  +  TALGVAE+ TPVLKESKF+ETGV+TPEEFVA+GD +V+HCPTWQWA GD ++ K+YLP DKQ+L+TR++PC +R   +   + Q E+++E +DPDGGWVD  H D     + EK SEM L+        D K+S +    NF+P   N G        DDD+DDD+EA+DM  F +     E+D       ++VI +    K  + S  +II TRTYDL ITYDK YQT RLWL GYDE R  LT EQM+ED SQDH KKTVTME HPH+PG   MASVHPC+ AEVMKK+I+ V EGG  LGVH Y++IFLKFVQAVIPTIEYDYT+NF M
Sbjct:    1 MQNVINTVKGTALGVAEYWTPVLKESKFRETGVLTPEEFVAAGDHLVHHCPTWQWAVGDETRIKSYLPHDKQFLLTRHVPCSRRCKQMEYCEEQ-EEIIEADDPDGGWVDTHHHDLGALGLDEKVSEMTLEPKTESAALDIKASGKTSKPNFAPSGNNNGTAVGGDDDDDDDDDDEEAIDMEQFEESGLLDEEDQ------NTVIASSNPTKEMEASAGEIIHTRTYDLHITYDKYYQTPRLWLSGYDEKRKPLTVEQMYEDVSQDHAKKTVTMETHPHMPGPP-MASVHPCKHAEVMKKIIQTVTEGGGELGVHMYLIIFLKFVQAVIPTIEYDYTQNFAM 334
[Close]
BLAST of Autophagy-related protein 3 vs. Ensembl Cavefish
Match: atg3 (autophagy related 3 [Source:NCBI gene;Acc:103036858])

HSP 1 Score: 340.117 bits (871), Expect = 1.959e-115
Identity = 186/329 (56.53%), Postives = 235/329 (71.43%), Query Frame = 3 
Query:  117 MHTMMQAMSRTALGVAEFLTPVLKESKFKETGVITPEEFVASGDFIVYHCPTWQWASGDPSKKKNYLPPDKQYLITRNLPCYKRVSHLFSNQNQNEKLVEPEDPDGGWVDAFHADESVSTITEKASEMILKDCKSSDERNFSPKHFNAGXXXXXXXXXXXAVDMAAFMKGECQFEDDDLI----APPTHSVIINKEILKAQDFSQSDIIQTRTYDLQITYDKLYQTCRLWLCGYDEDRSLLTEEQMFEDFSQDHVKKTVTMEVHPHLPGGVLMASVHPCRQAEVMKKLIEAVAEGGAVLGVHQYIVIFLKFVQAVIPTIEYDYTRNFNM 1091
            M  ++ ++  TALGVAEFLTPVLKESKFKETGVITPEEFVA+GD +V+HCPTW+WASG+ +K K YLP DKQ+L+TRN+PCYKR   +    ++ E ++E +D DGGWVD +H +  V+ +TE   E+ L +    D  N + +  +   D++ +DDDD+    AA M+   ++E+  L+    A    S I +    KA+   +  I+QTRTYDL ITYDK YQT RLWL GYDE+R  LT +QM+ED SQDHVKKTVT+E HPHLP    M SVHPCR AEVMKK+IE VAEGG  LGVH Y++IFLKFVQAVIPTIEYDYTR+F M
Sbjct:    1 MQNVINSVKGTALGVAEFLTPVLKESKFKETGVITPEEFVAAGDHLVHHCPTWKWASGEDTKVKPYLPHDKQFLLTRNVPCYKRCKQM-EYSDELEAIIEEDDGDGGWVDTYH-NSGVTGMTEAVREISLDN---KDNMNMNVRMRSGNGDNNGDDDDDDDEGEAADME---EYEESGLLETDEATLDTSKIADTNKCKAEAGGEDAILQTRTYDLYITYDKYYQTPRLWLFGYDEERQPLTVDQMYEDISQDHVKKTVTIENHPHLPPPA-MCSVHPCRHAEVMKKIIETVAEGGGELGVHMYLLIFLKFVQAVIPTIEYDYTRHFTM 320
[Close]
BLAST of Autophagy-related protein 3 vs. Ensembl Cavefish
Match: atg3 (autophagy related 3 [Source:NCBI gene;Acc:103036858])

HSP 1 Score: 331.643 bits (849), Expect = 3.837e-112
Identity = 181/329 (55.02%), Postives = 227/329 (69.00%), Query Frame = 3 
Query:  117 MHTMMQAMSRTALGVAEFLTPVLKESKFKETGVITPEEFVASGDFIVYHCPTWQWASGDPSKKKNYLPPDKQYLITRNLPCYKRVSHLFSNQNQNEKLVEPEDPDGGWVDAFHADESVSTITEKASEMILKDCKSSDERNFSPKHFNAGXXXXXXXXXXXAVDMAAFMKGECQFEDDDLI----APPTHSVIINKEILKAQDFSQSDIIQTRTYDLQITYDKLYQTCRLWLCGYDEDRSLLTEEQMFEDFSQDHVKKTVTMEVHPHLPGGVLMASVHPCRQAEVMKKLIEAVAEGGAVLGVHQYIVIFLKFVQAVIPTIEYDYTRNFNM 1091
            M  ++ ++  TALGVAEFLTPVLKESKFKETGVITPEEFVA+GD +V+HCPTW+WASG+ +K K YLP DKQ+L+TRN+PCYKR   +    ++ E ++E +D DGGWVD +H              ++       D  N + +  +   D++ +DDDD+    AA M+   ++E+  L+    A    S I +    KA+   +  I+QTRTYDL ITYDK YQT RLWL GYDE+R  LT +QM+ED SQDHVKKTVT+E HPHLP    M SVHPCR AEVMKK+IE VAEGG  LGVH Y++IFLKFVQAVIPTIEYDYTR+F M
Sbjct:    1 MQNVINSVKGTALGVAEFLTPVLKESKFKETGVITPEEFVAAGDHLVHHCPTWKWASGEDTKVKPYLPHDKQFLLTRNVPCYKRCKQM-EYSDELEAIIEEDDGDGGWVDTYHNSGETLIAIGYHQCLLCVSLLLKDNMNMNVRMRSGNGDNNGDDDDDDDEGEAADME---EYEESGLLETDEATLDTSKIADTNKCKAEAGGEDAILQTRTYDLYITYDKYYQTPRLWLFGYDEERQPLTVDQMYEDISQDHVKKTVTIENHPHLPPPA-MCSVHPCRHAEVMKKIIETVAEGGGELGVHMYLLIFLKFVQAVIPTIEYDYTRHFTM 324
[Close]
BLAST of Autophagy-related protein 3 vs. Ensembl Yeast
Match: ATG3 (E2-like enzyme; involved in autophagy and cytoplasm-to-vacuole targeting (Cvt) pathway; plays a role in formation of Atg8p-phosphatidylethanolamine conjugates, which are involved in membrane dynamics during autophagy and Cvt; interaction with Atg8p regulated by acetylation by NuA4 histone acetyltransferase Esa1p while attenuation of Atg3 acetylation is mediated by histone deacetylase Rpd3p; Atg12p-Atg5p conjugate enhances E2 activity of Atg3p by rearranging its catalytic site [Source:SGD;Acc:S000005290])

HSP 1 Score: 92.8189 bits (229), Expect = 9.211e-22
Identity = 51/145 (35.17%), Postives = 75/145 (51.72%), Query Frame = 3 
Query:  723 DIIQTRTYDLQITYDKLYQTCRLWLCGYDEDRSLLTEEQMFEDFSQDHVKKTVTMEVHPHLPGGVLMASVHPCRQAEVMKKLIEAVAEGGAV---------------------------LGVHQYIVIFLKFVQAVIPTIEYDYT 1076
            D+ Q R YDL I Y   Y+  ++++ G++ + S L+ EQMFED S D+  KT T+E  P     VL  S+HPC+ A VMK L++ V                                 L V QY+++FLKF+ +V P+I++DYT
Sbjct:  162 DMAQERYYDLYIAYSTSYRVPKMYIVGFNSNGSPLSPEQMFEDISADYRTKTATIEKLPFYKNSVLSVSIHPCKHANVMKILLDKVRVVRQRRRKELQEEQELDGVGDWEDLQDDIDDSLRVDQYLIVFLKFITSVTPSIQHDYT 306

HSP 2 Score: 76.6406 bits (187), Expect = 4.057e-16
Identity = 44/101 (43.56%), Postives = 60/101 (59.41%), Query Frame = 3 
Query:  138 MSRTALGV-AEFLTPVLKESKFKETGVITPEEFVASGDFIVYHCPTWQW--ASGDPSKKKNYLPPDKQYLITRNLPCYKRVSHLFSNQNQNEKLVEPEDPD 431
            M R+ L    E+LTP+  +S F  TG ITPEEFV +GD++ +  PTW+W   S D S  +++LP +KQ+LI R +PC KR           E+ VE E PD
Sbjct:    1 MIRSTLSSWREYLTPITHKSTFLTTGQITPEEFVQAGDYLCHMFPTWKWNEESSDIS-YRDFLPKNKQFLIIRKVPCDKRA----------EQCVEVEGPD 90
[Close]
BLAST of Autophagy-related protein 3 vs. Ensembl Nematostella
Match: EDO45196 (Autophagy-related protein 3 [Source:UniProtKB/TrEMBL;Acc:A7RTE4])

HSP 1 Score: 320.087 bits (819), Expect = 5.546e-108
Identity = 177/332 (53.31%), Postives = 228/332 (68.67%), Query Frame = 3 
Query:  117 MHTMMQAMSRTALGVAEFLTPVLKESKFKETGVITPEEFVASGDFIVYHCPTWQWASGDPSKKKNYLPPDKQYLITRNLPCYKRVSHLFSNQNQNEKLVEPEDPDGGWVDAFH-----ADESVSTITEKASEMILKDCKSSDERNFSPKHFNAGXXXXXXXXXXXAVDMAAFMKGECQFEDDD-LIAPPTHSVIINKEILKAQDFSQSD-IIQTRTYDLQITYDKLYQTCRLWLCGYDEDRSLLTEEQMFEDFSQDHVKKTVTMEVHPHLPGGVLMASVHPCRQAEVMKKLIEAVAEGGAVLGVHQYIVIFLKFVQAVIPTIEYDYTRNFNM 1091
            M  +   +  TALGVAE+LTPVLKESKF+ETGVITPEEFVA+GD +V+HCPTWQW+ G+ S+ K YLP DKQYL TRN+PCYKR   +  +Q +NE +VEP D DGGWVD  H      ++  + + E+ SEM L   K       +   F   ++DDD+++DDE  +     +     E+D+  +A P   V  ++      D +  + I+QTRTYD+ ITYDK YQT RLWL GY+E+R  L+ E+M+ED SQDH KKTVT+E HPHLP  + MASVHPCR A+VMKK+I+ VA+GG  L V+ Y++IFLKFVQAVIPTIEYDYTR F M
Sbjct:    1 MQDVFNKVKGTALGVAEYLTPVLKESKFQETGVITPEEFVAAGDHLVHHCPTWQWSMGEESRVKPYLPKDKQYLYTRNVPCYKRCKQM-EHQEENEAIVEP-DEDGGWVDTHHKVDPSGEKVTAGVQEQFSEMKLDSDKVPLLHCHALDDFLIRENDDDDEEDDEEAEDMEAFEESGMLENDEATLAVPVAPVSQSESSDGGTDATSGEGILQTRTYDMYITYDKYYQTPRLWLYGYNENRKPLSVEEMYEDMSQDHAKKTVTIEAHPHLP--MTMASVHPCRHADVMKKIIQTVADGGGELSVYMYLLIFLKFVQAVIPTIEYDYTRQFTM 328
[Close]
BLAST of Autophagy-related protein 3 vs. Ensembl Medaka
Match: atg3 (autophagy related 3 [Source:NCBI gene;Acc:101165197])

HSP 1 Score: 301.982 bits (772), Expect = 9.544e-101
Identity = 174/330 (52.73%), Postives = 216/330 (65.45%), Query Frame = 3 
Query:  117 MHTMMQAMSRTALGVAEFLTPVLKESKFKETGVITPEEFVASGDFIVYHCPTWQWASGDPSKKKNYLPPDKQYLITRNLPCYKRVSHLFSNQNQNEKLVEPEDPDGGWVDAFHADESVSTITEKASEMILKDCKSSDERNFSPKHFNAGXXXXXXXXXXXAVDMAAFMKGECQFEDDDLIAPP-----THSVIINKEILKAQDFSQSDIIQTRTYDLQITYDKLYQTCRLWLCGYDEDRSLLTEEQMFEDFSQDHVKKTVTMEVHPHLPGGVLMASVHPCRQAEVMKKLIEAVAEGGAVLGVHQYIVIFLKFVQAVIPTIEYDYTRNFNM 1091
            M  ++  +  TALGVAEFLTPVLK   +          FVA+GD +V+HCPTW+WA+G+ +K K +LPPDKQ+L+TRN+PCYKR   +    ++ E ++E +D DGGWVD +H   S  T    A    L+D           K+ NA      +DDD++    AA M+   ++E+  L+        TH V  +K   KA+  S+  I+QTRTYDL ITYDK YQT RLWL GYDEDR  LT EQM+ED SQDHVKKTVT+E HPHLP    M SVHPCR AEVMKK+IE VAEGG  LGVH Y++IFLKFVQAVIPTIEYDYTR+F M
Sbjct:    1 MQNVINTVKGTALGVAEFLTPVLKVGDW----------FVAAGDHLVHHCPTWKWAAGEEAKVKPFLPPDKQFLLTRNVPCYKRCKQM-EYSDELEAIIEEDDGDGGWVDTYH--NSGRTPNTPACVHRLQD-----------KNMNATSAPCCDDDDEDEEGEAADME---EYEESGLLETDEATLDTHRVTESK--AKAEPGSEDAILQTRTYDLYITYDKYYQTPRLWLFGYDEDRQPLTVEQMYEDISQDHVKKTVTIENHPHLPPPA-MCSVHPCRHAEVMKKIIETVAEGGGELGVHMYLLIFLKFVQAVIPTIEYDYTRHFTM 300
[Close]
BLAST of Autophagy-related protein 3 vs. Planmine SMEST
Match: SMESG000062512.1 (SMESG000062512.1)

HSP 1 Score: 657.522 bits (1695), Expect = 0.000e+0
Identity = 325/325 (100.00%), Postives = 325/325 (100.00%), Query Frame = 3 
Query:  117 MHTMMQAMSRTALGVAEFLTPVLKESKFKETGVITPEEFVASGDFIVYHCPTWQWASGDPSKKKNYLPPDKQYLITRNLPCYKRVSHLFSNQNQNEKLVEPEDPDGGWVDAFHADESVSTITEKASEMILKDCKSSDERNFSPKHFNAGXXXXXXXXXXXAVDMAAFMKGECQFEDDDLIAPPTHSVIINKEILKAQDFSQSDIIQTRTYDLQITYDKLYQTCRLWLCGYDEDRSLLTEEQMFEDFSQDHVKKTVTMEVHPHLPGGVLMASVHPCRQAEVMKKLIEAVAEGGAVLGVHQYIVIFLKFVQAVIPTIEYDYTRNFNM 1091
            MHTMMQAMSRTALGVAEFLTPVLKESKFKETGVITPEEFVASGDFIVYHCPTWQWASGDPSKKKNYLPPDKQYLITRNLPCYKRVSHLFSNQNQNEKLVEPEDPDGGWVDAFHADESVSTITEKASEMILKDCKSSDERNFSPKHFNAGDDDDDEDDDDEAVDMAAFMKGECQFEDDDLIAPPTHSVIINKEILKAQDFSQSDIIQTRTYDLQITYDKLYQTCRLWLCGYDEDRSLLTEEQMFEDFSQDHVKKTVTMEVHPHLPGGVLMASVHPCRQAEVMKKLIEAVAEGGAVLGVHQYIVIFLKFVQAVIPTIEYDYTRNFNM
Sbjct:    1 MHTMMQAMSRTALGVAEFLTPVLKESKFKETGVITPEEFVASGDFIVYHCPTWQWASGDPSKKKNYLPPDKQYLITRNLPCYKRVSHLFSNQNQNEKLVEPEDPDGGWVDAFHADESVSTITEKASEMILKDCKSSDERNFSPKHFNAGDDDDDEDDDDEAVDMAAFMKGECQFEDDDLIAPPTHSVIINKEILKAQDFSQSDIIQTRTYDLQITYDKLYQTCRLWLCGYDEDRSLLTEEQMFEDFSQDHVKKTVTMEVHPHLPGGVLMASVHPCRQAEVMKKLIEAVAEGGAVLGVHQYIVIFLKFVQAVIPTIEYDYTRNFNM 325
[Close]
BLAST of Autophagy-related protein 3 vs. Planmine SMEST
Match: SMESG000062512.1 (SMESG000062512.1)

HSP 1 Score: 650.973 bits (1678), Expect = 0.000e+0
Identity = 322/322 (100.00%), Postives = 322/322 (100.00%), Query Frame = 3 
Query:  126 MMQAMSRTALGVAEFLTPVLKESKFKETGVITPEEFVASGDFIVYHCPTWQWASGDPSKKKNYLPPDKQYLITRNLPCYKRVSHLFSNQNQNEKLVEPEDPDGGWVDAFHADESVSTITEKASEMILKDCKSSDERNFSPKHFNAGXXXXXXXXXXXAVDMAAFMKGECQFEDDDLIAPPTHSVIINKEILKAQDFSQSDIIQTRTYDLQITYDKLYQTCRLWLCGYDEDRSLLTEEQMFEDFSQDHVKKTVTMEVHPHLPGGVLMASVHPCRQAEVMKKLIEAVAEGGAVLGVHQYIVIFLKFVQAVIPTIEYDYTRNFNM 1091
            MMQAMSRTALGVAEFLTPVLKESKFKETGVITPEEFVASGDFIVYHCPTWQWASGDPSKKKNYLPPDKQYLITRNLPCYKRVSHLFSNQNQNEKLVEPEDPDGGWVDAFHADESVSTITEKASEMILKDCKSSDERNFSPKHFNAGDDDDDEDDDDEAVDMAAFMKGECQFEDDDLIAPPTHSVIINKEILKAQDFSQSDIIQTRTYDLQITYDKLYQTCRLWLCGYDEDRSLLTEEQMFEDFSQDHVKKTVTMEVHPHLPGGVLMASVHPCRQAEVMKKLIEAVAEGGAVLGVHQYIVIFLKFVQAVIPTIEYDYTRNFNM
Sbjct:    1 MMQAMSRTALGVAEFLTPVLKESKFKETGVITPEEFVASGDFIVYHCPTWQWASGDPSKKKNYLPPDKQYLITRNLPCYKRVSHLFSNQNQNEKLVEPEDPDGGWVDAFHADESVSTITEKASEMILKDCKSSDERNFSPKHFNAGDDDDDEDDDDEAVDMAAFMKGECQFEDDDLIAPPTHSVIINKEILKAQDFSQSDIIQTRTYDLQITYDKLYQTCRLWLCGYDEDRSLLTEEQMFEDFSQDHVKKTVTMEVHPHLPGGVLMASVHPCRQAEVMKKLIEAVAEGGAVLGVHQYIVIFLKFVQAVIPTIEYDYTRNFNM 322
[Close]
BLAST of Autophagy-related protein 3 vs. Planmine SMEST
Match: SMESG000062512.1 (SMESG000062512.1)

HSP 1 Score: 648.662 bits (1672), Expect = 0.000e+0
Identity = 321/321 (100.00%), Postives = 321/321 (100.00%), Query Frame = 3 
Query:  129 MQAMSRTALGVAEFLTPVLKESKFKETGVITPEEFVASGDFIVYHCPTWQWASGDPSKKKNYLPPDKQYLITRNLPCYKRVSHLFSNQNQNEKLVEPEDPDGGWVDAFHADESVSTITEKASEMILKDCKSSDERNFSPKHFNAGXXXXXXXXXXXAVDMAAFMKGECQFEDDDLIAPPTHSVIINKEILKAQDFSQSDIIQTRTYDLQITYDKLYQTCRLWLCGYDEDRSLLTEEQMFEDFSQDHVKKTVTMEVHPHLPGGVLMASVHPCRQAEVMKKLIEAVAEGGAVLGVHQYIVIFLKFVQAVIPTIEYDYTRNFNM 1091
            MQAMSRTALGVAEFLTPVLKESKFKETGVITPEEFVASGDFIVYHCPTWQWASGDPSKKKNYLPPDKQYLITRNLPCYKRVSHLFSNQNQNEKLVEPEDPDGGWVDAFHADESVSTITEKASEMILKDCKSSDERNFSPKHFNAGDDDDDEDDDDEAVDMAAFMKGECQFEDDDLIAPPTHSVIINKEILKAQDFSQSDIIQTRTYDLQITYDKLYQTCRLWLCGYDEDRSLLTEEQMFEDFSQDHVKKTVTMEVHPHLPGGVLMASVHPCRQAEVMKKLIEAVAEGGAVLGVHQYIVIFLKFVQAVIPTIEYDYTRNFNM
Sbjct:    1 MQAMSRTALGVAEFLTPVLKESKFKETGVITPEEFVASGDFIVYHCPTWQWASGDPSKKKNYLPPDKQYLITRNLPCYKRVSHLFSNQNQNEKLVEPEDPDGGWVDAFHADESVSTITEKASEMILKDCKSSDERNFSPKHFNAGDDDDDEDDDDEAVDMAAFMKGECQFEDDDLIAPPTHSVIINKEILKAQDFSQSDIIQTRTYDLQITYDKLYQTCRLWLCGYDEDRSLLTEEQMFEDFSQDHVKKTVTMEVHPHLPGGVLMASVHPCRQAEVMKKLIEAVAEGGAVLGVHQYIVIFLKFVQAVIPTIEYDYTRNFNM 321
The following BLAST results are available for this feature:
BLAST of Autophagy-related protein 3 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 3
Match NameE-valueIdentityDescription
ATG35.231e-10854.41autophagy related 3 [Source:HGNC Symbol;Acc:HGNC:2... [more]
ATG32.059e-9250.47autophagy related 3 [Source:HGNC Symbol;Acc:HGNC:2... [more]
ATG35.096e-2767.14autophagy related 3 [Source:HGNC Symbol;Acc:HGNC:2... [more]
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BLAST of Autophagy-related protein 3 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 2
Match NameE-valueIdentityDescription
atg-39.408e-4655.07Autophagy-related protein 3 [Source:UniProtKB/TrE... [more]
atg-31.650e-3560.82Autophagy-related protein 3 [Source:UniProtKB/TrE... [more]
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BLAST of Autophagy-related protein 3 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Atg32.228e-10751.03gene:FBgn0036813 transcript:FBtr0075055[more]
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BLAST of Autophagy-related protein 3 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
atg35.093e-11756.62autophagy related 3 [Source:NCBI gene;Acc:336632][more]
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BLAST of Autophagy-related protein 3 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 3
Match NameE-valueIdentityDescription
rnf11.25.846e-11256.84ring finger protein 11.2 [Source:Xenbase;Acc:XB-GE... [more]
rnf11.23.618e-9752.89ring finger protein 11.2 [Source:Xenbase;Acc:XB-GE... [more]
rnf11.26.984e-9554.19ring finger protein 11.2 [Source:Xenbase;Acc:XB-GE... [more]
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BLAST of Autophagy-related protein 3 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Atg31.843e-10953.85autophagy related 3 [Source:MGI Symbol;Acc:MGI:191... [more]
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BLAST of Autophagy-related protein 3 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q6PFS7|ATG3_DANRE3.845e-11656.62Ubiquitin-like-conjugating enzyme ATG3 OS=Danio re... [more]
sp|Q5I0S6|ATG3_XENTR3.097e-11156.84Ubiquitin-like-conjugating enzyme ATG3 OS=Xenopus ... [more]
sp|Q6GQE7|ATG3_XENLA7.361e-11056.71Ubiquitin-like-conjugating enzyme ATG3 OS=Xenopus ... [more]
sp|Q9CPX6|ATG3_MOUSE1.289e-10853.85Ubiquitin-like-conjugating enzyme ATG3 OS=Mus musc... [more]
sp|Q9NT62|ATG3_HUMAN2.512e-10754.41Ubiquitin-like-conjugating enzyme ATG3 OS=Homo sap... [more]
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BLAST of Autophagy-related protein 3 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A220XHQ40.000e+088.92Autophagy-related protein 3 OS=Dugesia japonica OX... [more]
K1R9341.764e-12159.94Autophagy-related protein 3 OS=Crassostrea gigas O... [more]
A0A1S3JZA04.366e-12159.76Autophagy-related protein 3 OS=Lingula unguis OX=7... [more]
A0A210QTW86.437e-11761.09Autophagy-related protein 3 OS=Mizuhopecten yessoe... [more]
A0A3Q8C0P21.091e-11655.56Autophagy-related protein 3 OS=Locusta migratoria ... [more]
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BLAST of Autophagy-related protein 3 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
atg31.959e-11556.53autophagy related 3 [Source:NCBI gene;Acc:10303685... [more]
atg33.837e-11255.02autophagy related 3 [Source:NCBI gene;Acc:10303685... [more]
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BLAST of Autophagy-related protein 3 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Autophagy-related protein 3 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 1
Match NameE-valueIdentityDescription
ATG39.211e-2235.17E2-like enzyme; involved in autophagy and cytoplas... [more]
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BLAST of Autophagy-related protein 3 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO451965.546e-10853.31Autophagy-related protein 3 [Source:UniProtKB/TrE... [more]
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BLAST of Autophagy-related protein 3 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
atg39.544e-10152.73autophagy related 3 [Source:NCBI gene;Acc:10116519... [more]
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BLAST of Autophagy-related protein 3 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 3
Match NameE-valueIdentityDescription
SMESG000062512.10.000e+0100.00SMESG000062512.1[more]
SMESG000062512.10.000e+0100.00SMESG000062512.1[more]
SMESG000062512.10.000e+0100.00SMESG000062512.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30023558 ID=SMED30023558|Name=Autophagy-related protein 3|organism=Schmidtea mediterranea sexual|type=transcript|length=1191bp
TTCAGAATTATATTGTAAATTCTTAAAAATTATTTATATGAATAAATTTT
TAAATAAATTTTCCTAAAAATATTATTAATAAGAATTTTATAAATTTTGA
TTTAGTTTTGTATAAAATGCATACAATGATGCAAGCTATGAGTAGAACAG
CTTTAGGGGTGGCTGAATTTTTAACTCCCGTATTAAAAGAATCGAAATTT
AAGGAAACTGGCGTAATAACTCCTGAGGAATTTGTTGCTTCTGGTGATTT
TATTGTTTATCATTGTCCAACTTGGCAATGGGCTTCCGGTGATCCTTCTA
AAAAGAAGAACTATCTACCACCCGATAAGCAGTATCTTATAACTAGAAAT
TTACCGTGTTATAAAAGAGTGAGTCATTTATTTTCAAATCAGAACCAAAA
TGAAAAATTAGTGGAACCGGAAGATCCGGATGGCGGTTGGGTTGATGCTT
TTCATGCTGATGAATCTGTTTCAACGATAACTGAAAAAGCCTCTGAAATG
ATTTTAAAAGATTGCAAAAGTTCAGATGAAAGAAATTTTTCTCCAAAACA
CTTTAACGCCGGTGATGATGACGACGATGAAGATGACGATGACGAAGCTG
TTGATATGGCAGCGTTTATGAAAGGGGAATGCCAATTTGAAGATGACGAT
TTAATAGCTCCTCCGACACATTCAGTTATAATCAATAAAGAAATTCTTAA
AGCTCAAGATTTCTCTCAGTCGGATATTATCCAAACGCGAACGTATGACT
TACAGATTACTTATGATAAATTATATCAGACATGTCGCTTATGGCTTTGT
GGTTATGATGAGGATCGATCGTTGCTCACAGAAGAGCAAATGTTTGAAGA
TTTTAGTCAGGATCATGTAAAGAAAACCGTGACAATGGAAGTCCATCCAC
ATTTACCAGGAGGTGTATTAATGGCATCTGTCCATCCCTGTCGCCAGGCG
GAAGTGATGAAGAAATTGATAGAAGCCGTAGCAGAAGGCGGTGCTGTTTT
AGGGGTTCATCAATACATTGTGATTTTTTTGAAATTTGTCCAAGCTGTTA
TTCCTACAATCGAGTATGATTACACAAGAAATTTTAATATGTGAATTTTC
AACGCCATGTTTCAATATTTAATTGCATTATATAATTTGATCCATTAAGC
CTTGATTTGTGGTAAATAAAATCTTTATTGGGAAAAAAAAA
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protein sequence of SMED30023558-orf-1

>SMED30023558-orf-1 ID=SMED30023558-orf-1|Name=SMED30023558-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=326bp
MHTMMQAMSRTALGVAEFLTPVLKESKFKETGVITPEEFVASGDFIVYHC
PTWQWASGDPSKKKNYLPPDKQYLITRNLPCYKRVSHLFSNQNQNEKLVE
PEDPDGGWVDAFHADESVSTITEKASEMILKDCKSSDERNFSPKHFNAGD
DDDDEDDDDEAVDMAAFMKGECQFEDDDLIAPPTHSVIINKEILKAQDFS
QSDIIQTRTYDLQITYDKLYQTCRLWLCGYDEDRSLLTEEQMFEDFSQDH
VKKTVTMEVHPHLPGGVLMASVHPCRQAEVMKKLIEAVAEGGAVLGVHQY
IVIFLKFVQAVIPTIEYDYTRNFNM*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000034epidermis
PLANA:0000070intestinal phagocyte
PLANA:0000099neuron
PLANA:0003116parenchymal cell
Vocabulary: INTERPRO
TermDefinition
IPR007134Autophagy-rel_prot_3_N
IPR019461Autophagy-rel_prot_3_C
IPR007135Autophagy-rel_prot_3
Vocabulary: cellular component
TermDefinition
GO:0005737cytoplasm
Vocabulary: biological process
TermDefinition
GO:0006810transport
GO:0006914autophagy
GO:0015031protein transport
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR019461Autophagy-related protein 3, C-terminalPFAMPF10381Autophagy_Ccoord: 297..320
e-value: 1.4E-15
score: 56.5
IPR007134Autophagy-related protein 3, N-terminalPFAMPF03986Autophagy_Ncoord: 10..153
e-value: 3.6E-46
score: 156.5
IPR007135Autophagy-related protein 3PFAMPF03987Autophagy_act_Ccoord: 214..276
e-value: 2.2E-14
score: 53.8
NoneNo IPR availableGENE3DG3DSA:3.30.1460.50coord: 27..135
e-value: 2.4E-24
score: 88.3
NoneNo IPR availableGENE3DG3DSA:3.30.1460.50coord: 161..325
e-value: 1.7E-54
score: 186.4
NoneNo IPR availablePANTHERPTHR12866AUTOPHAGOCYTOSIS PROTEIN AUT1-RELATEDcoord: 1..321
NoneNo IPR availablePANTHERPTHR12866:SF2UBIQUITIN-LIKE-CONJUGATING ENZYME ATG3coord: 1..321