SMED30023450

Overview


Name	SMED30023450
Smed ID	SMED30023450
Length (bp)	489

Neoblast Clusters

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30023450 (SMED30023450) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30023450 (SMED30023450) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30023450

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 4

Expressed In	Reference Transcript	Gene Models	Published Transcript	Transcriptome	Publication
head region	SMED30023450	SMESG000062901.1	SmedASXL_001357	SmedAsxl_ww_GCZZ01	PMID:27034770 Currie et al., 2016
parenchyma	SMED30023450	SMESG000062901.1	dd_Smed_v4_11053_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
parenchyma	SMED30023450	SMESG000062901.1	KF487096	smed_ncbi_20200123	PMID:24173799 Cowles et al., 2013
secretory system	SMED30023450	SMESG000062901.1	dd_Smed_v6_11053_0	dd_Smed_v6	PMID:29674432 Plass et al., 2018

Note: Hover over icons to view figure legend

Alignments

SMED30023450 aligns in the following genomic locations:


Alignment Location	Alignment Score
v31.009130:2377..2909 -	2409

Homology

[Close]

BLAST of SMED30023450 vs. Ensembl Human
Match: FERD3L (Fer3 like bHLH transcription factor [Source:HGNC Symbol;Acc:HGNC:16660])

HSP 1 Score: 87.0409 bits (214), Expect = 1.241e-21
Identity = 42/53 (79.25%), Postives = 44/53 (83.02%), Query Frame = -3

Query:   89 AHRTAANIRERKRMINLNAAFENLRLKVPTFVFEKRLSRIETLRLAIAYIKFM 247
            A R AANIRERKRM NLN AF+ LR KVPTF +EKRLSRIETLRLAI YI FM
Sbjct:  101 AQRQAANIRERKRMFNLNEAFDQLRRKVPTFAYEKRLSRIETLRLAIVYISFM 153

[Close]

BLAST of SMED30023450 vs. Ensembl Human
Match: PTF1A (pancreas associated transcription factor 1a [Source:HGNC Symbol;Acc:HGNC:23734])

HSP 1 Score: 73.9442 bits (180), Expect = 1.376e-15
Identity = 32/60 (53.33%), Postives = 44/60 (73.33%), Query Frame = -3

Query:   62 RTAANIRERKRMINLNAAFENLRLKVPTFVFEKRLSRIETLRLAIAYIKFMECILNGVWP 241
            R AAN+RER+RM ++N AFE LR  +PT  +EKRLS+++TLRLAI YI F+  ++    P
Sbjct:  165 RQAANVRERRRMQSINDAFEGLRSHIPTLPYEKRLSKVDTLRLAIGYINFLSELVQADLP 224

[Close]

BLAST of SMED30023450 vs. Ensembl Human
Match: NEUROD2 (neuronal differentiation 2 [Source:HGNC Symbol;Acc:HGNC:7763])

HSP 1 Score: 56.9954 bits (136), Expect = 1.254e-9
Identity = 30/55 (54.55%), Postives = 37/55 (67.27%), Query Frame = -3

Query:   77 RTAANIRERKRMINLNAAFENLRLKVPTFVFEKRLSRIETLRLAIAYIKFMECIL 241
            R  AN RER RM +LNAA +NLR  VP +   ++LS+IETLRLA  YI  +  IL
Sbjct:  123 RQKANARERNRMHDLNAALDNLRKVVPCYSKTQKLSKIETLRLAKNYIWALSEIL 177

[Close]

BLAST of SMED30023450 vs. Ensembl Human
Match: NEUROD1 (neuronal differentiation 1 [Source:HGNC Symbol;Acc:HGNC:7762])

HSP 1 Score: 56.6102 bits (135), Expect = 1.729e-9
Identity = 30/55 (54.55%), Postives = 36/55 (65.45%), Query Frame = -3

Query:   77 RTAANIRERKRMINLNAAFENLRLKVPTFVFEKRLSRIETLRLAIAYIKFMECIL 241
            R  AN RER RM  LNAA +NLR  VP +   ++LS+IETLRLA  YI  +  IL
Sbjct:  103 RMKANARERNRMHGLNAALDNLRKVVPCYSKTQKLSKIETLRLAKNYIWALSEIL 157

[Close]

BLAST of SMED30023450 vs. Ensembl Human
Match: ATOH1 (atonal bHLH transcription factor 1 [Source:HGNC Symbol;Acc:HGNC:797])

HSP 1 Score: 55.8398 bits (133), Expect = 3.614e-9
Identity = 27/56 (48.21%), Postives = 38/56 (67.86%), Query Frame = -3

Query:   74 RTAANIRERKRMINLNAAFENLRLKVPTFVFEKRLSRIETLRLAIAYIKFMECILN 241
            R AAN RER+RM  LN AF+ LR  +P+F  +K+LS+ ETL++A  YI  +  +L 
Sbjct:  161 RLAANARERRRMHGLNHAFDQLRNVIPSFNNDKKLSKYETLQMAQIYINALSELLQ 216

[Close]

BLAST of SMED30023450 vs. Ensembl Celegans
Match: hlh-13 (Helix-loop-helix protein 13 [Source:UniProtKB/Swiss-Prot;Acc:Q20561])

HSP 1 Score: 67.781 bits (164), Expect = 8.544e-15
Identity = 32/56 (57.14%), Postives = 42/56 (75.00%), Query Frame = -3

Query:   77 HRTAANIRERKRMINLNAAFENLRLKVPTFVFEKRLSRIETLRLAIAYIKFMECIL 244
             R  A+IRERKRM ++N AF  LR  +PTF +EKRLS+I+TL LAIAYI  ++ +L
Sbjct:   42 ERQTASIRERKRMCSINVAFIELRNYIPTFPYEKRLSKIDTLNLAIAYINMLDDVL 97

[Close]

BLAST of SMED30023450 vs. Ensembl Celegans
Match: ngn-1 (NeuroGeNin [Source:UniProtKB/TrEMBL;Acc:Q95XG7])

HSP 1 Score: 53.5286 bits (127), Expect = 3.324e-9
Identity = 28/63 (44.44%), Postives = 38/63 (60.32%), Query Frame = -3

Query:   62 TAHRTAANIRERKRMINLNAAFENLRLKVPTFVFEKRLSRIETLRLAIAYIKFMECILNGVWP 250
            T  R  AN RER+RM +LN A E+LR  +P    E ++++IETLR A  YI  +   L+G  P
Sbjct:   61 TVRRDKANARERRRMNSLNDALEHLRGILPALPDEPKMTKIETLRKAQEYIASLSFQLSGGSP 123

[Close]

BLAST of SMED30023450 vs. Ensembl Celegans
Match: hlh-15 (Helix Loop Helix [Source:UniProtKB/TrEMBL;Acc:Q18590])

HSP 1 Score: 51.6026 bits (122), Expect = 3.437e-9
Identity = 26/57 (45.61%), Postives = 36/57 (63.16%), Query Frame = -3

Query:   74 HRTAANIRERKRMINLNAAFENLRLKVPTFVFEKRLSRIETLRLAIAYIKFMECILN 244
            +R     RER R+ + N AF  LR  +PT   EK+LS+IE LR +IAYI F++ +L 
Sbjct:   33 YRNLHATRERIRVESFNMAFSQLRALLPTLPVEKKLSKIEILRFSIAYISFLDNLLQ 89

[Close]

BLAST of SMED30023450 vs. Ensembl Celegans
Match: lin-32 (pep chromosome:WBcel235:X:2229208:2230122:-1 gene:WBGene00003018.1 transcript:T14F9.5.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:lin-32)

HSP 1 Score: 51.6026 bits (122), Expect = 8.776e-9
Identity = 26/55 (47.27%), Postives = 37/55 (67.27%), Query Frame = -3

Query:   77 RTAANIRERKRMINLNAAFENLRLKVPTFVFEKRLSRIETLRLAIAYIKFMECIL 241
            R+AAN RER+RM  LN A++ LR  +P     K+LS+ ETL++A  YI+ +  IL
Sbjct:   74 RSAANERERRRMNTLNVAYDELREVLPEIDSGKKLSKFETLQMAQKYIECLSQIL 128

[Close]

BLAST of SMED30023450 vs. Ensembl Celegans
Match: hlh-6 (Helix-loop-helix protein 6 [Source:UniProtKB/Swiss-Prot;Acc:Q10007])

HSP 1 Score: 51.6026 bits (122), Expect = 2.835e-8
Identity = 25/51 (49.02%), Postives = 36/51 (70.59%), Query Frame = -3

Query:   77 NIRERKRMINLNAAFENLRLKVPTFVFEKRLSRIETLRLAIAYIKFMECIL 229
            N RER R+ N+N  +E LR  +P    EKR+S+++TLRLAI YIK ++ +L
Sbjct:  179 NERERCRVRNVNDGYERLRKHLPVHFDEKRISKVDTLRLAIRYIKHLDNLL 229

[Close]

BLAST of SMED30023450 vs. Ensembl Fly
Match: Fer3 (gene:FBgn0037937 transcript:FBtr0082435)

HSP 1 Score: 91.2781 bits (225), Expect = 3.078e-23
Identity = 44/62 (70.97%), Postives = 49/62 (79.03%), Query Frame = -3

Query:   68 STAHRTAANIRERKRMINLNAAFENLRLKVPTFVFEKRLSRIETLRLAIAYIKFMECILNGV 253
            S A R AANIRER+RM NLN AF+ LR KVPTF +EKRLSRIETLRLAI YI FM  +L+G 
Sbjct:   84 SMAQRRAANIRERRRMFNLNEAFDKLRRKVPTFAYEKRLSRIETLRLAITYIGFMAELLSGT 145

[Close]

BLAST of SMED30023450 vs. Ensembl Fly
Match: Fer1 (gene:FBgn0037475 transcript:FBtr0081567)

HSP 1 Score: 73.559 bits (179), Expect = 3.691e-16
Identity = 33/68 (48.53%), Postives = 49/68 (72.06%), Query Frame = -3

Query:   53 HRTAANIRERKRMINLNAAFENLRLKVPTFVFEKRLSRIETLRLAIAYIKFMECIL----NGVWPKIT 244
             R AAN+RER+RM ++N AFE LR  +PT  +EKRLS+++TL+LAI+YI F+  ++    NG  P ++
Sbjct:   82 QRQAANLRERRRMQSINEAFEGLRTHIPTLPYEKRLSKVDTLKLAISYITFLSEMVKKDKNGNEPGLS 149

[Close]

BLAST of SMED30023450 vs. Ensembl Fly
Match: Fer1 (gene:FBgn0037475 transcript:FBtr0334670)

HSP 1 Score: 73.1738 bits (178), Expect = 5.253e-16
Identity = 33/68 (48.53%), Postives = 49/68 (72.06%), Query Frame = -3

Query:   53 HRTAANIRERKRMINLNAAFENLRLKVPTFVFEKRLSRIETLRLAIAYIKFMECIL----NGVWPKIT 244
             R AAN+RER+RM ++N AFE LR  +PT  +EKRLS+++TL+LAI+YI F+  ++    NG  P ++
Sbjct:   87 QRQAANLRERRRMQSINEAFEGLRTHIPTLPYEKRLSKVDTLKLAISYITFLSEMVKKDKNGNEPGLS 154

[Close]

BLAST of SMED30023450 vs. Ensembl Fly
Match: Fer2 (gene:FBgn0038402 transcript:FBtr0301709)

HSP 1 Score: 71.2478 bits (173), Expect = 3.748e-15
Identity = 35/64 (54.69%), Postives = 49/64 (76.56%), Query Frame = -3

Query:   53 RTAANIRERKRM-INLNAAFENLRLKVPTFVFEKRLSRIETLRLAIAYIKFMECILNGVWPKIT 241
            R AAN+RERKR+  ++N+AF+ LR+ VPTF +EKRLS+I+TLRLAIAYI  +  +L   +  +T
Sbjct:  149 RQAANVRERKRIQSSINSAFDELRVHVPTFPYEKRLSKIDTLRLAIAYISLLREVLQTDYDPLT 212

[Close]

BLAST of SMED30023450 vs. Ensembl Fly
Match: Fer2 (gene:FBgn0038402 transcript:FBtr0344012)

HSP 1 Score: 70.8626 bits (172), Expect = 4.321e-15
Identity = 35/64 (54.69%), Postives = 49/64 (76.56%), Query Frame = -3

Query:   53 RTAANIRERKRM-INLNAAFENLRLKVPTFVFEKRLSRIETLRLAIAYIKFMECILNGVWPKIT 241
            R AAN+RERKR+  ++N+AF+ LR+ VPTF +EKRLS+I+TLRLAIAYI  +  +L   +  +T
Sbjct:  149 RQAANVRERKRIQSSINSAFDELRVHVPTFPYEKRLSKIDTLRLAIAYISLLREVLQTDYDPLT 212

[Close]

BLAST of SMED30023450 vs. Ensembl Zebrafish
Match: ferd3l (Fer3-like bHLH transcription factor [Source:ZFIN;Acc:ZDB-GENE-081104-461])

HSP 1 Score: 85.8853 bits (211), Expect = 2.214e-22
Identity = 43/59 (72.88%), Postives = 47/59 (79.66%), Query Frame = -3

Query:   77 STAHRTAANIRERKRMINLNAAFENLRLKVPTFVFEKRLSRIETLRLAIAYIKFMECIL 253
            ST  R AANIRERKRM +LN AF+ LR +VPTF +EKRLSRIETLRLAI YI FM  IL
Sbjct:   22 STVQRQAANIRERKRMFSLNEAFDRLRRRVPTFAYEKRLSRIETLRLAIVYIAFMTDIL 80

[Close]

BLAST of SMED30023450 vs. Ensembl Zebrafish
Match: si:dkey-34f9.3 (si:dkey-34f9.3 [Source:ZFIN;Acc:ZDB-GENE-081104-410])

HSP 1 Score: 83.5741 bits (205), Expect = 1.380e-20
Identity = 41/63 (65.08%), Postives = 51/63 (80.95%), Query Frame = -3

Query:   59 HRTAANIRERKRMINLNAAFENLRLKVPTFVFEKRLSRIETLRLAIAYIKFM-ECILNGVWPK 244
             R AANIRERKRM+++N+AFE LR  VPTF +EKRLS+I+TLRLAIAYI  + E +L+G  PK
Sbjct:   66 QRHAANIRERKRMLSINSAFEELRCHVPTFPYEKRLSKIDTLRLAIAYIALLREILLSGCDPK 128

[Close]

BLAST of SMED30023450 vs. Ensembl Zebrafish
Match: ptf1a (pancreas associated transcription factor 1a [Source:NCBI gene;Acc:368662])

HSP 1 Score: 73.1738 bits (178), Expect = 7.324e-16
Identity = 32/60 (53.33%), Postives = 44/60 (73.33%), Query Frame = -3

Query:   62 RTAANIRERKRMINLNAAFENLRLKVPTFVFEKRLSRIETLRLAIAYIKFMECILNGVWP 241
            R AAN+RER+RM ++N AFE LR  +PT  +EKRLS+++TLRLAI YI F+  ++    P
Sbjct:  117 RQAANVRERRRMQSINDAFEGLRSHIPTLPYEKRLSKVDTLRLAIGYINFLAELVQSDMP 176

[Close]

BLAST of SMED30023450 vs. Ensembl Zebrafish
Match: atoh7 (atonal bHLH transcription factor 7 [Source:NCBI gene;Acc:58216])

HSP 1 Score: 58.5362 bits (140), Expect = 3.116e-11
Identity = 29/56 (51.79%), Postives = 40/56 (71.43%), Query Frame = -3

Query:   74 RTAANIRERKRMINLNAAFENLRLKVPTFVFEKRLSRIETLRLAIAYIKFMECILN 241
            R AAN RERKRM  LN AF+ LR  VP +  +K+LS+ ETL++A++YI  +  IL+
Sbjct:   30 RMAANARERKRMQGLNTAFDRLRKVVPQWGQDKKLSKYETLQMALSYIMALNRILS 85

[Close]

BLAST of SMED30023450 vs. Ensembl Zebrafish
Match: AL807792.2 (pep chromosome:GRCz11:CHR_ALT_CTG8_2_5:29594098:29599935:1 gene:ENSDARG00000110597.1 transcript:ENSDART00000185012.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:AL807792.2)

HSP 1 Score: 59.3066 bits (142), Expect = 1.040e-10
Identity = 29/61 (47.54%), Postives = 39/61 (63.93%), Query Frame = -3

Query:   59 RTAANIRERKRMINLNAAFENLRLKVPTFVFEKRLSRIETLRLAIAYIKFMECILNGVWPK 241
            R AAN RER+RM  LN AF+ LR  +P F  +K+LS+ ETL++A  YI  +  +L G   K
Sbjct:  120 RMAANARERRRMHGLNHAFDELRSVIPAFDNDKKLSKYETLQMAQIYINALSDLLQGPGAK 180

[Close]

BLAST of SMED30023450 vs. Ensembl Xenopus
Match: ferd3l (Fer3-like bHLH transcription factor [Source:NCBI gene;Acc:100124312])

HSP 1 Score: 85.1149 bits (209), Expect = 2.192e-21
Identity = 43/57 (75.44%), Postives = 46/57 (80.70%), Query Frame = -3

Query:   77 AHRTAANIRERKRMINLNAAFENLRLKVPTFVFEKRLSRIETLRLAIAYIKFMECIL 247
            A R AANIRERKRM NLN AF+ LR KVPTF +EKRLSRIETLRLAI YI FM  +L
Sbjct:   65 AQRQAANIRERKRMFNLNEAFDLLRKKVPTFAYEKRLSRIETLRLAIVYISFMTEML 121

[Close]

BLAST of SMED30023450 vs. Ensembl Xenopus
Match: ENSXETT00000031930.1 (pep primary_assembly:Xenopus_tropicalis_v9.1:9:55646956:55647657:1 gene:ENSXETG00000008961.1 transcript:ENSXETT00000031930.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 83.9593 bits (206), Expect = 1.745e-20
Identity = 41/63 (65.08%), Postives = 53/63 (84.13%), Query Frame = -3

Query:   59 HRTAANIRERKRMINLNAAFENLRLKVPTFVFEKRLSRIETLRLAIAYIKFMECIL-NGVWPK 244
            HR AANIRER+RM+++N+AFE LR +VPTF +EKRLS+I+TLRLAIAYI  +  IL +G+ PK
Sbjct:   84 HRQAANIRERRRMLSINSAFEELRGRVPTFPYEKRLSKIDTLRLAIAYIALLSDILSSGMDPK 146

[Close]

BLAST of SMED30023450 vs. Ensembl Xenopus
Match: PTF1A (pancreas associated transcription factor 1a [Source:NCBI gene;Acc:100494161])

HSP 1 Score: 72.7886 bits (177), Expect = 2.789e-15
Identity = 32/60 (53.33%), Postives = 44/60 (73.33%), Query Frame = -3

Query:   62 RTAANIRERKRMINLNAAFENLRLKVPTFVFEKRLSRIETLRLAIAYIKFMECILNGVWP 241
            R AAN+RER+RM ++N AFE LR  +PT  +EKRLS+++TLRLAI YI F+  ++    P
Sbjct:  171 RQAANVRERRRMQSINDAFEGLRSHIPTLPYEKRLSKVDTLRLAIGYINFLSELVQSDLP 230

[Close]

BLAST of SMED30023450 vs. Ensembl Xenopus
Match: ppil6 (peptidylprolyl isomerase like 6 [Source:Xenbase;Acc:XB-GENE-979458])

HSP 1 Score: 59.6918 bits (143), Expect = 1.581e-11
Identity = 28/52 (53.85%), Postives = 39/52 (75.00%), Query Frame = -3

Query:   98 STAHRTAANIRERKRMINLNAAFENLRLKVPTFVFEKRLSRIETLRLAIAYI 253
            ST  R AAN RER+RM  LN AF++LR  VP +  +K+LS+ ETL++A++YI
Sbjct:   32 STKRRMAANARERRRMQGLNTAFDSLRKVVPQWGEDKKLSKYETLQMALSYI 83

[Close]

BLAST of SMED30023450 vs. Ensembl Xenopus
Match: neurod1 (neuronal differentiation 1 [Source:NCBI gene;Acc:100038290])

HSP 1 Score: 56.9954 bits (136), Expect = 1.380e-9
Identity = 30/55 (54.55%), Postives = 36/55 (65.45%), Query Frame = -3

Query:   77 RTAANIRERKRMINLNAAFENLRLKVPTFVFEKRLSRIETLRLAIAYIKFMECIL 241
            R  AN RER RM  LNAA +NLR  VP +   ++LS+IETLRLA  YI  +  IL
Sbjct:  110 RMKANARERNRMHGLNAALDNLRKVVPCYSKTQKLSKIETLRLAKNYIWALSEIL 164

[Close]

BLAST of SMED30023450 vs. Ensembl Mouse
Match: Ferd3l (Fer3 like bHLH transcription factor [Source:MGI Symbol;Acc:MGI:2150010])

HSP 1 Score: 87.4261 bits (215), Expect = 5.346e-22
Identity = 43/58 (74.14%), Postives = 46/58 (79.31%), Query Frame = -3

Query:   74 AHRTAANIRERKRMINLNAAFENLRLKVPTFVFEKRLSRIETLRLAIAYIKFMECILN 247
            A R AANIRERKRM NLN AF+ LR KVPTF +EKRLSRIETLRLAI YI FM  +L 
Sbjct:  103 AQRQAANIRERKRMFNLNEAFDQLRRKVPTFAYEKRLSRIETLRLAIVYISFMTELLQ 160

[Close]

BLAST of SMED30023450 vs. Ensembl Mouse
Match: Ptf1a (pancreas specific transcription factor, 1a [Source:MGI Symbol;Acc:MGI:1328312])

HSP 1 Score: 73.559 bits (179), Expect = 1.216e-15
Identity = 32/60 (53.33%), Postives = 44/60 (73.33%), Query Frame = -3

Query:   62 RTAANIRERKRMINLNAAFENLRLKVPTFVFEKRLSRIETLRLAIAYIKFMECILNGVWP 241
            R AAN+RER+RM ++N AFE LR  +PT  +EKRLS+++TLRLAI YI F+  ++    P
Sbjct:  162 RQAANVRERRRMQSINDAFEGLRSHIPTLPYEKRLSKVDTLRLAIGYINFLSELVQADLP 221

[Close]

BLAST of SMED30023450 vs. Ensembl Mouse
Match: Neurod2 (neurogenic differentiation 2 [Source:MGI Symbol;Acc:MGI:107755])

HSP 1 Score: 57.3806 bits (137), Expect = 7.979e-10
Identity = 30/55 (54.55%), Postives = 37/55 (67.27%), Query Frame = -3

Query:   77 RTAANIRERKRMINLNAAFENLRLKVPTFVFEKRLSRIETLRLAIAYIKFMECIL 241
            R  AN RER RM +LNAA +NLR  VP +   ++LS+IETLRLA  YI  +  IL
Sbjct:  124 RQKANARERNRMHDLNAALDNLRKVVPCYSKTQKLSKIETLRLAKNYIWALSEIL 178

[Close]

BLAST of SMED30023450 vs. Ensembl Mouse
Match: Neurod1 (neurogenic differentiation 1 [Source:MGI Symbol;Acc:MGI:1339708])

HSP 1 Score: 56.6102 bits (135), Expect = 1.235e-9
Identity = 30/55 (54.55%), Postives = 36/55 (65.45%), Query Frame = -3

Query:   77 RTAANIRERKRMINLNAAFENLRLKVPTFVFEKRLSRIETLRLAIAYIKFMECIL 241
            R  AN RER RM  LNAA +NLR  VP +   ++LS+IETLRLA  YI  +  IL
Sbjct:  103 RMKANARERNRMHGLNAALDNLRKVVPCYSKTQKLSKIETLRLAKNYIWALSEIL 157

[Close]

BLAST of SMED30023450 vs. Ensembl Mouse
Match: Atoh1 (atonal bHLH transcription factor 1 [Source:MGI Symbol;Acc:MGI:104654])

HSP 1 Score: 55.8398 bits (133), Expect = 2.180e-9
Identity = 27/56 (48.21%), Postives = 38/56 (67.86%), Query Frame = -3

Query:   74 RTAANIRERKRMINLNAAFENLRLKVPTFVFEKRLSRIETLRLAIAYIKFMECILN 241
            R AAN RER+RM  LN AF+ LR  +P+F  +K+LS+ ETL++A  YI  +  +L 
Sbjct:  158 RLAANARERRRMHGLNHAFDQLRNVIPSFNNDKKLSKYETLQMAQIYINALSELLQ 213

[Close]

BLAST of SMED30023450 vs. UniProt/SwissProt
Match: sp|Q9VGJ5|FER3_DROME (Protein Fer3 OS=Drosophila melanogaster OX=7227 GN=fer3 PE=2 SV=2)

HSP 1 Score: 91.2781 bits (225), Expect = 3.085e-22
Identity = 44/62 (70.97%), Postives = 49/62 (79.03%), Query Frame = -3

Query:   68 STAHRTAANIRERKRMINLNAAFENLRLKVPTFVFEKRLSRIETLRLAIAYIKFMECILNGV 253
            S A R AANIRER+RM NLN AF+ LR KVPTF +EKRLSRIETLRLAI YI FM  +L+G 
Sbjct:   84 SMAQRRAANIRERRRMFNLNEAFDKLRRKVPTFAYEKRLSRIETLRLAITYIGFMAELLSGT 145

[Close]

BLAST of SMED30023450 vs. UniProt/SwissProt
Match: sp|Q923Z4|FER3L_MOUSE (Fer3-like protein OS=Mus musculus OX=10090 GN=Ferd3l PE=1 SV=1)

HSP 1 Score: 87.4261 bits (215), Expect = 3.739e-21
Identity = 43/58 (74.14%), Postives = 46/58 (79.31%), Query Frame = -3

Query:   74 AHRTAANIRERKRMINLNAAFENLRLKVPTFVFEKRLSRIETLRLAIAYIKFMECILN 247
            A R AANIRERKRM NLN AF+ LR KVPTF +EKRLSRIETLRLAI YI FM  +L 
Sbjct:  103 AQRQAANIRERKRMFNLNEAFDQLRRKVPTFAYEKRLSRIETLRLAIVYISFMTELLQ 160

[Close]

BLAST of SMED30023450 vs. UniProt/SwissProt
Match: sp|Q96RJ6|FER3L_HUMAN (Fer3-like protein OS=Homo sapiens OX=9606 GN=FERD3L PE=1 SV=1)

HSP 1 Score: 87.0409 bits (214), Expect = 5.961e-21
Identity = 42/53 (79.25%), Postives = 44/53 (83.02%), Query Frame = -3

Query:   89 AHRTAANIRERKRMINLNAAFENLRLKVPTFVFEKRLSRIETLRLAIAYIKFM 247
            A R AANIRERKRM NLN AF+ LR KVPTF +EKRLSRIETLRLAI YI FM
Sbjct:  101 AQRQAANIRERKRMFNLNEAFDQLRRKVPTFAYEKRLSRIETLRLAIVYISFM 153

[Close]

BLAST of SMED30023450 vs. UniProt/SwissProt
Match: sp|Q4ZHW1|PTF1A_XENLA (Pancreas transcription factor 1 subunit alpha OS=Xenopus laevis OX=8355 GN=ptf1a PE=2 SV=1)

HSP 1 Score: 73.9442 bits (180), Expect = 3.853e-15
Identity = 31/51 (60.78%), Postives = 41/51 (80.39%), Query Frame = -3

Query:   89 RTAANIRERKRMINLNAAFENLRLKVPTFVFEKRLSRIETLRLAIAYIKFM 241
            R AAN+RER+RM ++N AFE LR  +PT  +EKRLS+++TLRLAI YI F+
Sbjct:  121 RQAANVRERRRMQSINDAFEGLRSHIPTLPYEKRLSKVDTLRLAIGYINFL 171

[Close]

BLAST of SMED30023450 vs. UniProt/SwissProt
Match: sp|Q7ZSX3|PTF1A_DANRE (Pancreas transcription factor 1 subunit alpha OS=Danio rerio OX=7955 GN=ptf1a PE=2 SV=1)

HSP 1 Score: 73.1738 bits (178), Expect = 5.529e-15
Identity = 32/60 (53.33%), Postives = 44/60 (73.33%), Query Frame = -3

Query:   62 RTAANIRERKRMINLNAAFENLRLKVPTFVFEKRLSRIETLRLAIAYIKFMECILNGVWP 241
            R AAN+RER+RM ++N AFE LR  +PT  +EKRLS+++TLRLAI YI F+  ++    P
Sbjct:  117 RQAANVRERRRMQSINDAFEGLRSHIPTLPYEKRLSKVDTLRLAIGYINFLAELVQSDMP 176

[Close]

BLAST of SMED30023450 vs. TrEMBL
Match: U5YR58 (Fer3l-3 protein (Fragment) OS=Schmidtea mediterranea OX=79327 GN=fer3l-3 PE=2 SV=1)

HSP 1 Score: 112.464 bits (280), Expect = 1.302e-29
Identity = 70/70 (100.00%), Postives = 70/70 (100.00%), Query Frame = -3

Query:  140 RNPSHFSTVKELEKYDSTXXXXXXXXXXXXXKSTAHRTAANIRERKRMINLNAAFENLRLKVPTFVFEKR 349
            RNPSHFSTVKELEKYDSTKCKKYQKQKRRRNKSTAHRTAANIRERKRMINLNAAFENLRLKVPTFVFEKR
Sbjct:    1 RNPSHFSTVKELEKYDSTKCKKYQKQKRRRNKSTAHRTAANIRERKRMINLNAAFENLRLKVPTFVFEKR 70

[Close]

BLAST of SMED30023450 vs. TrEMBL
Match: A0A267EYW1 (BHLH domain-containing protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig000937g1 PE=4 SV=1)

HSP 1 Score: 97.8265 bits (242), Expect = 8.703e-22
Identity = 46/64 (71.88%), Postives = 52/64 (81.25%), Query Frame = -3

Query:   62 STAHRTAANIRERKRMINLNAAFENLRLKVPTFVFEKRLSRIETLRLAIAYIKFMECILNGVWP 253
            S A R AAN+RER+RM NLN AF+ LR KVPTF FEKRLSRIETLRLAI YI+FM  ++NGV P
Sbjct:  127 SQAQRKAANVRERRRMFNLNEAFDTLRTKVPTFAFEKRLSRIETLRLAITYIRFMGQVINGVPP 190

[Close]

BLAST of SMED30023450 vs. TrEMBL
Match: A0A267EJA0 (BHLH domain-containing protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig000937g2 PE=4 SV=1)

HSP 1 Score: 97.8265 bits (242), Expect = 8.703e-22
Identity = 46/64 (71.88%), Postives = 52/64 (81.25%), Query Frame = -3

Query:   62 STAHRTAANIRERKRMINLNAAFENLRLKVPTFVFEKRLSRIETLRLAIAYIKFMECILNGVWP 253
            S A R AAN+RER+RM NLN AF+ LR KVPTF FEKRLSRIETLRLAI YI+FM  ++NGV P
Sbjct:  127 SQAQRKAANVRERRRMFNLNEAFDTLRTKVPTFAFEKRLSRIETLRLAITYIRFMGQVINGVPP 190

[Close]

BLAST of SMED30023450 vs. TrEMBL
Match: A0A267FMG9 (BHLH domain-containing protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig000937g4 PE=4 SV=1)

HSP 1 Score: 97.8265 bits (242), Expect = 9.760e-22
Identity = 46/64 (71.88%), Postives = 52/64 (81.25%), Query Frame = -3

Query:   62 STAHRTAANIRERKRMINLNAAFENLRLKVPTFVFEKRLSRIETLRLAIAYIKFMECILNGVWP 253
            S A R AAN+RER+RM NLN AF+ LR KVPTF FEKRLSRIETLRLAI YI+FM  ++NGV P
Sbjct:  127 SQAQRKAANVRERRRMFNLNEAFDTLRTKVPTFAFEKRLSRIETLRLAITYIRFMGQVINGVPP 190

[Close]

BLAST of SMED30023450 vs. TrEMBL
Match: A0A1I8Q5E2 (BHLH domain-containing protein OS=Stomoxys calcitrans OX=35570 GN=106094457 PE=4 SV=1)

HSP 1 Score: 95.5153 bits (236), Expect = 2.435e-21
Identity = 46/64 (71.88%), Postives = 51/64 (79.69%), Query Frame = -3

Query:   62 STAHRTAANIRERKRMINLNAAFENLRLKVPTFVFEKRLSRIETLRLAIAYIKFMECILNGVWP 253
            S A R AANIRER+RM+NLN AF+ LR KVPTF +EKRLSRIETLRLAI YI FM  +LNG  P
Sbjct:   91 SLAQRRAANIRERRRMLNLNEAFDKLRPKVPTFAYEKRLSRIETLRLAITYIGFMTELLNGTPP 154

[Close]

BLAST of SMED30023450 vs. Ensembl Cavefish
Match: ferd3l (Fer3-like bHLH transcription factor [Source:ZFIN;Acc:ZDB-GENE-081104-461])

HSP 1 Score: 84.3445 bits (207), Expect = 6.102e-22
Identity = 41/55 (74.55%), Postives = 45/55 (81.82%), Query Frame = -3

Query:   89 STAHRTAANIRERKRMINLNAAFENLRLKVPTFVFEKRLSRIETLRLAIAYIKFM 253
            +T  R AANIRERKRM +LN AF+ LR KVPTF +EKRLSRIETLRLAI YI FM
Sbjct:   13 TTVQRQAANIRERKRMFSLNEAFDELRKKVPTFAYEKRLSRIETLRLAIVYISFM 67

[Close]

BLAST of SMED30023450 vs. Ensembl Cavefish
Match: si:dkey-34f9.3 (neurogenic differentiation factor 1-like [Source:NCBI gene;Acc:107197807])

HSP 1 Score: 83.1889 bits (204), Expect = 6.478e-20
Identity = 40/63 (63.49%), Postives = 51/63 (80.95%), Query Frame = -3

Query:   59 HRTAANIRERKRMINLNAAFENLRLKVPTFVFEKRLSRIETLRLAIAYIKFM-ECILNGVWPK 244
             R AANIRERKRM+++N+AFE LR  VPTF +EKRLS+I+TLRLAIAYI  + E +++G  PK
Sbjct:  121 QRHAANIRERKRMLSINSAFEQLRCHVPTFPYEKRLSKIDTLRLAIAYIALLREILMSGCDPK 183

[Close]

BLAST of SMED30023450 vs. Ensembl Cavefish
Match: ptf1a (pancreas associated transcription factor 1a [Source:NCBI gene;Acc:103046795])

HSP 1 Score: 72.4034 bits (176), Expect = 1.102e-15
Identity = 31/51 (60.78%), Postives = 41/51 (80.39%), Query Frame = -3

Query:   89 RTAANIRERKRMINLNAAFENLRLKVPTFVFEKRLSRIETLRLAIAYIKFM 241
            R AAN+RER+RM ++N AFE LR  +PT  +EKRLS+++TLRLAI YI F+
Sbjct:   94 RQAANVRERRRMQSINDAFEGLRSHIPTLPYEKRLSKVDTLRLAIGYINFL 144

[Close]

BLAST of SMED30023450 vs. Ensembl Cavefish
Match: atoh1b (atonal bHLH transcription factor 1b [Source:ZFIN;Acc:ZDB-GENE-041201-1])

HSP 1 Score: 57.3806 bits (137), Expect = 3.816e-10
Identity = 28/58 (48.28%), Postives = 39/58 (67.24%), Query Frame = -3

Query:   68 RTAANIRERKRMINLNAAFENLRLKVPTFVFEKRLSRIETLRLAIAYIKFMECILNGV 241
            R AAN RER+RM  LN AF+ LR  +P+   EK+LS+ +TL++A  YI  +  +L GV
Sbjct:  104 RVAANARERRRMHGLNRAFDKLRSVIPSLENEKKLSKYDTLQMAQIYIAELSELLEGV 161

[Close]

BLAST of SMED30023450 vs. Ensembl Cavefish
Match: scxb (basic helix-loop-helix transcription factor scleraxis-like [Source:NCBI gene;Acc:103029811])

HSP 1 Score: 56.225 bits (134), Expect = 5.213e-10
Identity = 28/56 (50.00%), Postives = 38/56 (67.86%), Query Frame = -3

Query:   77 HRTAANIRERKRMINLNAAFENLRLKVPTFVFEKRLSRIETLRLAIAYIKFMECIL 244
             R AAN RER R  ++N AF  LR  +PT   +++LS+IETLRLA +YI  +E +L
Sbjct:   79 QRNAANARERDRTNSVNVAFTALRTLIPTEPADRKLSKIETLRLASSYISHLENVL 134

[Close]

BLAST of SMED30023450 vs. Ensembl Sea Lamprey
Match: si:dkey-34f9.3 (si:dkey-34f9.3 [Source:ZFIN;Acc:ZDB-GENE-081104-410])

HSP 1 Score: 80.4925 bits (197), Expect = 1.988e-20
Identity = 37/56 (66.07%), Postives = 47/56 (83.93%), Query Frame = -3

Query:   77 HRTAANIRERKRMINLNAAFENLRLKVPTFVFEKRLSRIETLRLAIAYIKFMECIL 244
             RTAAN+RER+RM+++N+AFE LR  VPTF +EKRLS+I+TLRLAIAYI  +  IL
Sbjct:   34 QRTAANVRERRRMLSINSAFEELRCHVPTFPYEKRLSKIDTLRLAIAYIALLRDIL 89

[Close]

BLAST of SMED30023450 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000004072.1 (pep scaffold:Pmarinus_7.0:GL492732:1750:4325:-1 gene:ENSPMAG00000003727.1 transcript:ENSPMAT00000004072.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 75.485 bits (184), Expect = 2.383e-17
Identity = 32/51 (62.75%), Postives = 41/51 (80.39%), Query Frame = -3

Query:   89 RTAANIRERKRMINLNAAFENLRLKVPTFVFEKRLSRIETLRLAIAYIKFM 241
            R AAN RER+RM ++NAAFE LR  +PT  +EKRLS+++TLRLAI YI F+
Sbjct:  133 RQAANQRERRRMQSINAAFEGLRAHIPTLPYEKRLSKVDTLRLAIGYISFL 183

[Close]

BLAST of SMED30023450 vs. Ensembl Sea Lamprey
Match: neurod1 (neuronal differentiation 1 [Source:NCBI gene;Acc:30169])

HSP 1 Score: 55.4546 bits (132), Expect = 3.835e-10
Identity = 30/55 (54.55%), Postives = 36/55 (65.45%), Query Frame = -3

Query:   77 RTAANIRERKRMINLNAAFENLRLKVPTFVFEKRLSRIETLRLAIAYIKFMECIL 241
            R  AN RER RM  LNAA +NLR  VP +   ++LS+IETLRLA  YI  +  IL
Sbjct:   25 RVKANARERNRMHGLNAALDNLRKVVPCYSKTQKLSKIETLRLAKNYIWALSEIL 79

[Close]

BLAST of SMED30023450 vs. Ensembl Sea Lamprey
Match: TWIST1 (twist family bHLH transcription factor 1 [Source:HGNC Symbol;Acc:HGNC:12428])

HSP 1 Score: 51.2174 bits (121), Expect = 1.694e-9
Identity = 27/58 (46.55%), Postives = 38/58 (65.52%), Query Frame = -3

Query:   71 HRTAANIRERKRMINLNAAFENLRLKVPTFVFEKRLSRIETLRLAIAYIKFMECILNG 244
             R  AN+RER+R  +LN AF +LR  +PT   +K LS+I+TL+LA  YI F+  +L  
Sbjct:    9 QRVLANVRERQRTQSLNEAFASLRKIIPTLPSDK-LSKIQTLKLASRYIDFLYQVLQS 65

[Close]

BLAST of SMED30023450 vs. Ensembl Sea Lamprey
Match: TWIST1 (twist family bHLH transcription factor 1 [Source:HGNC Symbol;Acc:HGNC:12428])

HSP 1 Score: 52.7582 bits (125), Expect = 2.000e-9
Identity = 27/56 (48.21%), Postives = 38/56 (67.86%), Query Frame = -3

Query:   77 HRTAANIRERKRMINLNAAFENLRLKVPTFVFEKRLSRIETLRLAIAYIKFMECIL 244
             R  AN+RER+R  +LN AF +LR  +PT   +K LS+I+TL+LA  YI F+  +L
Sbjct:   96 QRFVANVRERQRTQSLNEAFASLRQIIPTLPSDK-LSKIQTLKLATRYIDFLYQVL 150

[Close]

BLAST of SMED30023450 vs. Ensembl Nematostella
Match: EDO43849 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RXI0])

HSP 1 Score: 70.8626 bits (172), Expect = 3.349e-17
Identity = 32/53 (60.38%), Postives = 41/53 (77.36%), Query Frame = -3

Query:   89 AHRTAANIRERKRMINLNAAFENLRLKVPTFVFEKRLSRIETLRLAIAYIKFM 247
              R AAN+RERKRM ++N AFE LR  +PT  +EKRLS+++TLRLAI YI F+
Sbjct:    2 VQRHAANLRERKRMQSINEAFEGLRKHIPTLPYEKRLSKVDTLRLAIGYIGFL 54

[Close]

BLAST of SMED30023450 vs. Ensembl Nematostella
Match: EDO42924 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RZZ0])

HSP 1 Score: 58.5362 bits (140), Expect = 1.792e-12
Identity = 30/56 (53.57%), Postives = 39/56 (69.64%), Query Frame = -3

Query:   77 HRTAANIRERKRMINLNAAFENLRLKVPTFVFEKRLSRIETLRLAIAYIKFMECIL 244
             R AAN RER R  ++NAAF  LRL +PT   +++LS+IETLRLA +YI  +  IL
Sbjct:    2 QRQAANARERNRTHSVNAAFNALRLLIPTEPSDRKLSKIETLRLASSYIAHLSTIL 57

[Close]

BLAST of SMED30023450 vs. Ensembl Nematostella
Match: EDO42907 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S063])

HSP 1 Score: 53.1434 bits (126), Expect = 2.476e-10
Identity = 27/59 (45.76%), Postives = 39/59 (66.10%), Query Frame = -3

Query:   74 TAHRTAANIRERKRMINLNAAFENLRLKVPTFVFEKRLSRIETLRLAIAYIKFMECILN 250
            +  R  AN RER R+  +NAAFE LR K+P    EK+ S+I+T+RLA  YI+ +  +L+
Sbjct:    2 SKQRRTANERERNRVQQVNAAFETLRNKIPLRALEKKPSKIDTIRLATRYIQDLTQLLS 60

[Close]

BLAST of SMED30023450 vs. Ensembl Nematostella
Match: EDO40812 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S669])

HSP 1 Score: 52.373 bits (124), Expect = 3.854e-10
Identity = 24/57 (42.11%), Postives = 39/57 (68.42%), Query Frame = -3

Query:   71 RTAANIRERKRMINLNAAFENLRLKVPTFVFEKRLSRIETLRLAIAYIKFMECILNG 241
            +T  + +ER+R  N+NAAF  LR  +P    + +LS+I+TL+LA++YI  +E  L+G
Sbjct:    1 KTTTSKKERRRTENINAAFAELRKHIPNVPSDTKLSKIKTLKLAMSYIHHLELQLSG 57

[Close]

BLAST of SMED30023450 vs. Ensembl Nematostella
Match: EDO46193 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RQN3])

HSP 1 Score: 51.9878 bits (123), Expect = 6.409e-10
Identity = 27/58 (46.55%), Postives = 38/58 (65.52%), Query Frame = -3

Query:   68 RTAANIRERKRMINLNAAFENLRLKVPTFVFEKRLSRIETLRLAIAYIKFMECILNGV 241
            R   N RER R  ++N AF  LR  +PT+  E++LS+ E LR A+ YIKF+E IL+ +
Sbjct:    3 RLYTNSRERWRQQHVNLAFAELRKLIPTYPPERKLSKNEILRFAMKYIKFLENILSDM 60

[Close]

BLAST of SMED30023450 vs. Ensembl Medaka
Match: ferd3l (Fer3 like bHLH transcription factor [Source:NCBI gene;Acc:101167882])

HSP 1 Score: 81.6481 bits (200), Expect = 6.187e-21
Identity = 39/59 (66.10%), Postives = 46/59 (77.97%), Query Frame = -3

Query:   77 STAHRTAANIRERKRMINLNAAFENLRLKVPTFVFEKRLSRIETLRLAIAYIKFMECIL 253
            +   R AAN+RER+RM +LN AF+ LR KVPTF +EKRLSRIETLRLAI YI FM  +L
Sbjct:   12 TVVQRQAANVRERRRMFSLNEAFDELRRKVPTFAYEKRLSRIETLRLAIVYISFMTDLL 70

[Close]

BLAST of SMED30023450 vs. Ensembl Medaka
Match: si:dkey-34f9.3 (si:dkey-34f9.3 [Source:ZFIN;Acc:ZDB-GENE-081104-410])

HSP 1 Score: 80.4925 bits (197), Expect = 4.613e-19
Identity = 40/63 (63.49%), Postives = 51/63 (80.95%), Query Frame = -3

Query:   56 RTAANIRERKRMINLNAAFENLRLKVPTFVFEKRLSRIETLRLAIAYIKFM-ECILNGVWPKI 241
            R AANIRERKRM+++N+AFE LR  VPTF +EKRLS+I+TLRLAIAYI  + E +L+G   K+
Sbjct:  126 RHAANIRERKRMLSINSAFEELRCHVPTFPYEKRLSKIDTLRLAIAYIALLREILLSGATEKM 188

[Close]

BLAST of SMED30023450 vs. Ensembl Medaka
Match: ptf1a (pancreas associated transcription factor 1a [Source:NCBI gene;Acc:101160350])

HSP 1 Score: 73.9442 bits (180), Expect = 3.318e-16
Identity = 31/51 (60.78%), Postives = 41/51 (80.39%), Query Frame = -3

Query:   89 RTAANIRERKRMINLNAAFENLRLKVPTFVFEKRLSRIETLRLAIAYIKFM 241
            R AAN+RER+RM ++N AFE LR  +PT  +EKRLS+++TLRLAI YI F+
Sbjct:  120 RQAANVRERRRMQSINDAFEGLRSHIPTLPYEKRLSKVDTLRLAIGYINFL 170

[Close]

BLAST of SMED30023450 vs. Ensembl Medaka
Match: atoh7 (atonal bHLH transcription factor 7 [Source:NCBI gene;Acc:101155052])

HSP 1 Score: 59.6918 bits (143), Expect = 1.212e-11
Identity = 30/59 (50.85%), Postives = 41/59 (69.49%), Query Frame = -3

Query:   77 STAHRTAANIRERKRMINLNAAFENLRLKVPTFVFEKRLSRIETLRLAIAYIKFMECIL 253
            +T  R AAN RERKRM  LN AF+ LR  VP +  +K+LS+ ETL++A++YI  +  IL
Sbjct:   29 ATRRRMAANARERKRMQGLNTAFDRLRKVVPQWGQDKKLSKYETLQMALSYIMALSRIL 87

[Close]

BLAST of SMED30023450 vs. Ensembl Medaka
Match: atoh1b (neurogenin-2 [Source:NCBI gene;Acc:101175327])

HSP 1 Score: 58.151 bits (139), Expect = 1.406e-10
Identity = 28/58 (48.28%), Postives = 40/58 (68.97%), Query Frame = -3

Query:   68 RTAANIRERKRMINLNAAFENLRLKVPTFVFEKRLSRIETLRLAIAYIKFMECILNGV 241
            R AAN RER+RM  LN AF+ LR  +P+   EK+LS+ +TL++A  YI  +  +L+GV
Sbjct:   92 RVAANARERRRMHGLNKAFDELRSVIPSLENEKKLSKYDTLQMAQIYITELSELLSGV 149

[Close]

BLAST of SMED30023450 vs. Planmine SMEST
Match: SMESG000062901.1 (SMESG000062901.1)

HSP 1 Score: 213.386 bits (542), Expect = 2.535e-72
Identity = 121/122 (99.18%), Postives = 121/122 (99.18%), Query Frame = -3

Query:   35 MLDLKSDFSITTAKLLDRNPSHFSTVKELEKYDSTXXXXXXXXXXXXXKSTAHRTAANIRERKRMINLNAAFENLRLKVPTFVFEKRLSRIETLRLAIAYIKFMECILNGVWPKITN*FDKI 400
            MLDLKSDFSITTAKLLDRNPSHFSTVKELEKYDSTKCKKYQKQKRRRNKSTAHRTAANIRERKRMINLNAAFENLRLKVPTFVFEKRLSRIETLRLAIAYIKFMECILNGVWPKITN FDKI
Sbjct:    1 MLDLKSDFSITTAKLLDRNPSHFSTVKELEKYDSTKCKKYQKQKRRRNKSTAHRTAANIRERKRMINLNAAFENLRLKVPTFVFEKRLSRIETLRLAIAYIKFMECILNGVWPKITNSFDKI 122

[Close]

BLAST of SMED30023450 vs. Planmine SMEST
Match: SMESG000029000.1 (SMESG000029000.1)

HSP 1 Score: 87.4261 bits (215), Expect = 1.479e-21
Identity = 42/61 (68.85%), Postives = 50/61 (81.97%), Query Frame = -3

Query:   71 STAHRTAANIRERKRMINLNAAFENLRLKVPTFVFEKRLSRIETLRLAIAYIKFMECILNG 253
            + A R AANIRER+RM +LN AF++LR +VPTF +EKRLSRIETLRLAI YI FM  +LNG
Sbjct:  135 TVAQRRAANIRERRRMYSLNEAFDSLRKRVPTFAYEKRLSRIETLRLAITYICFMTELLNG 195

[Close]

BLAST of SMED30023450 vs. Planmine SMEST
Match: SMESG000029000.1 (SMESG000029000.1)

HSP 1 Score: 87.0409 bits (214), Expect = 1.937e-21
Identity = 42/61 (68.85%), Postives = 50/61 (81.97%), Query Frame = -3

Query:   71 STAHRTAANIRERKRMINLNAAFENLRLKVPTFVFEKRLSRIETLRLAIAYIKFMECILNG 253
            + A R AANIRER+RM +LN AF++LR +VPTF +EKRLSRIETLRLAI YI FM  +LNG
Sbjct:  142 TVAQRRAANIRERRRMYSLNEAFDSLRKRVPTFAYEKRLSRIETLRLAITYICFMTELLNG 202

[Close]

BLAST of SMED30023450 vs. Planmine SMEST
Match: SMESG000019274.1 (SMESG000019274.1)

HSP 1 Score: 78.5666 bits (192), Expect = 1.458e-18
Identity = 42/64 (65.62%), Postives = 49/64 (76.56%), Query Frame = -3

Query:   56 HRTAANIRERKRMINLNAAFENLRLKVPTFVFEKRLSRIETLRLAIAYIKFM-ECILNGVWPKI 244
             R AANIRER+RM NLN AF+ LR  VPTF +EK+LSRIETLRLAI YI FM E ++N   P+I
Sbjct:  116 QRHAANIRERRRMNNLNKAFDRLRQLVPTFAYEKQLSRIETLRLAITYISFMTEQLINA--PEI 177

[Close]

BLAST of SMED30023450 vs. Planmine SMEST
Match: SMESG000044960.1 (SMESG000044960.1)

HSP 1 Score: 74.7146 bits (182), Expect = 5.584e-17
Identity = 32/56 (57.14%), Postives = 44/56 (78.57%), Query Frame = -3

Query:   77 HRTAANIRERKRMINLNAAFENLRLKVPTFVFEKRLSRIETLRLAIAYIKFMECIL 244
             R AAN+RERKRM ++N AFE LR+ +PT  +EKRLS+++TLR+AI YI F+  I+
Sbjct:   47 QRQAANMRERKRMQSINDAFEKLRVHIPTMPYEKRLSKVDTLRMAIGYINFLGGIM 102

The following BLAST results are available for this feature:

BLAST of SMED30023450 vs. Ensembl Human
BLAST of SMED30023450 vs. Ensembl Celegans
BLAST of SMED30023450 vs. Ensembl Fly
BLAST of SMED30023450 vs. Ensembl Zebrafish
BLAST of SMED30023450 vs. Ensembl Xenopus
BLAST of SMED30023450 vs. Ensembl Mouse
BLAST of SMED30023450 vs. UniProt/SwissProt
BLAST of SMED30023450 vs. TrEMBL
BLAST of SMED30023450 vs. Ensembl Cavefish
BLAST of SMED30023450 vs. Ensembl Sea Lamprey
BLAST of SMED30023450 vs. Ensembl Yeast
BLAST of SMED30023450 vs. Ensembl Nematostella
BLAST of SMED30023450 vs. Ensembl Medaka
BLAST of SMED30023450 vs. Planmine SMEST

BLAST of SMED30023450 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5


Match Name	E-value	Identity	Description
FERD3L	1.241e-21	79.25	Fer3 like bHLH transcription factor [Source:HGNC S...	[more]
PTF1A	1.376e-15	53.33	pancreas associated transcription factor 1a [Sourc...	[more]
NEUROD2	1.254e-9	54.55	neuronal differentiation 2 [Source:HGNC Symbol;Acc...	[more]
NEUROD1	1.729e-9	54.55	neuronal differentiation 1 [Source:HGNC Symbol;Acc...	[more]
ATOH1	3.614e-9	48.21	atonal bHLH transcription factor 1 [Source:HGNC Sy...	[more]

BLAST of SMED30023450 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5


Match Name	E-value	Identity	Description
hlh-13	8.544e-15	57.14	Helix-loop-helix protein 13 [Source:UniProtKB/Swi...	[more]
ngn-1	3.324e-9	44.44	NeuroGeNin [Source:UniProtKB/TrEMBL;Acc:Q95XG7]	[more]
hlh-15	3.437e-9	45.61	Helix Loop Helix [Source:UniProtKB/TrEMBL;Acc:Q18...	[more]
lin-32	8.776e-9	47.27	pep chromosome:WBcel235:X:2229208:2230122:-1 gene:...	[more]
hlh-6	2.835e-8	49.02	Helix-loop-helix protein 6 [Source:UniProtKB/Swis...	[more]

BLAST of SMED30023450 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5


Match Name	E-value	Identity	Description
Fer3	3.078e-23	70.97	gene:FBgn0037937 transcript:FBtr0082435	[more]
Fer1	3.691e-16	48.53	gene:FBgn0037475 transcript:FBtr0081567	[more]
Fer1	5.253e-16	48.53	gene:FBgn0037475 transcript:FBtr0334670	[more]
Fer2	3.748e-15	54.69	gene:FBgn0038402 transcript:FBtr0301709	[more]
Fer2	4.321e-15	54.69	gene:FBgn0038402 transcript:FBtr0344012	[more]

BLAST of SMED30023450 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
ferd3l	2.214e-22	72.88	Fer3-like bHLH transcription factor [Source:ZFIN;A...	[more]
si:dkey-34f9.3	1.380e-20	65.08	si:dkey-34f9.3 [Source:ZFIN;Acc:ZDB-GENE-081104-41...	[more]
ptf1a	7.324e-16	53.33	pancreas associated transcription factor 1a [Sourc...	[more]
atoh7	3.116e-11	51.79	atonal bHLH transcription factor 7 [Source:NCBI ge...	[more]
AL807792.2	1.040e-10	47.54	pep chromosome:GRCz11:CHR_ALT_CTG8_2_5:29594098:29...	[more]

BLAST of SMED30023450 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5


Match Name	E-value	Identity	Description
ferd3l	2.192e-21	75.44	Fer3-like bHLH transcription factor [Source:NCBI g...	[more]
ENSXETT00000031930.1	1.745e-20	65.08	pep primary_assembly:Xenopus_tropicalis_v9.1:9:556...	[more]
PTF1A	2.789e-15	53.33	pancreas associated transcription factor 1a [Sourc...	[more]
ppil6	1.581e-11	53.85	peptidylprolyl isomerase like 6 [Source:Xenbase;Ac...	[more]
neurod1	1.380e-9	54.55	neuronal differentiation 1 [Source:NCBI gene;Acc:1...	[more]

BLAST of SMED30023450 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5


Match Name	E-value	Identity	Description
Ferd3l	5.346e-22	74.14	Fer3 like bHLH transcription factor [Source:MGI Sy...	[more]
Ptf1a	1.216e-15	53.33	pancreas specific transcription factor, 1a [Source...	[more]
Neurod2	7.979e-10	54.55	neurogenic differentiation 2 [Source:MGI Symbol;Ac...	[more]
Neurod1	1.235e-9	54.55	neurogenic differentiation 1 [Source:MGI Symbol;Ac...	[more]
Atoh1	2.180e-9	48.21	atonal bHLH transcription factor 1 [Source:MGI Sym...	[more]

BLAST of SMED30023450 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5


Match Name	E-value	Identity	Description
sp\|Q9VGJ5\|FER3_DROME	3.085e-22	70.97	Protein Fer3 OS=Drosophila melanogaster OX=7227 GN...	[more]
sp\|Q923Z4\|FER3L_MOUSE	3.739e-21	74.14	Fer3-like protein OS=Mus musculus OX=10090 GN=Ferd...	[more]
sp\|Q96RJ6\|FER3L_HUMAN	5.961e-21	79.25	Fer3-like protein OS=Homo sapiens OX=9606 GN=FERD3...	[more]
sp\|Q4ZHW1\|PTF1A_XENLA	3.853e-15	60.78	Pancreas transcription factor 1 subunit alpha OS=X...	[more]
sp\|Q7ZSX3\|PTF1A_DANRE	5.529e-15	53.33	Pancreas transcription factor 1 subunit alpha OS=D...	[more]

BLAST of SMED30023450 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
U5YR58	1.302e-29	100.00	Fer3l-3 protein (Fragment) OS=Schmidtea mediterran...	[more]
A0A267EYW1	8.703e-22	71.88	BHLH domain-containing protein OS=Macrostomum lign...	[more]
A0A267EJA0	8.703e-22	71.88	BHLH domain-containing protein OS=Macrostomum lign...	[more]
A0A267FMG9	9.760e-22	71.88	BHLH domain-containing protein OS=Macrostomum lign...	[more]
A0A1I8Q5E2	2.435e-21	71.88	BHLH domain-containing protein OS=Stomoxys calcitr...	[more]

BLAST of SMED30023450 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
ferd3l	6.102e-22	74.55	Fer3-like bHLH transcription factor [Source:ZFIN;A...	[more]
si:dkey-34f9.3	6.478e-20	63.49	neurogenic differentiation factor 1-like [Source:N...	[more]
ptf1a	1.102e-15	60.78	pancreas associated transcription factor 1a [Sourc...	[more]
atoh1b	3.816e-10	48.28	atonal bHLH transcription factor 1b [Source:ZFIN;A...	[more]
scxb	5.213e-10	50.00	basic helix-loop-helix transcription factor sclera...	[more]

BLAST of SMED30023450 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5


Match Name	E-value	Identity	Description
si:dkey-34f9.3	1.988e-20	66.07	si:dkey-34f9.3 [Source:ZFIN;Acc:ZDB-GENE-081104-41...	[more]
ENSPMAT00000004072.1	2.383e-17	62.75	pep scaffold:Pmarinus_7.0:GL492732:1750:4325:-1 ge...	[more]
neurod1	3.835e-10	54.55	neuronal differentiation 1 [Source:NCBI gene;Acc:3...	[more]
TWIST1	1.694e-9	46.55	twist family bHLH transcription factor 1 [Source:H...	[more]
TWIST1	2.000e-9	48.21	twist family bHLH transcription factor 1 [Source:H...	[more]

BLAST of SMED30023450 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30023450 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5


Match Name	E-value	Identity	Description
EDO43849	3.349e-17	60.38	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO42924	1.792e-12	53.57	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO42907	2.476e-10	45.76	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO40812	3.854e-10	42.11	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO46193	6.409e-10	46.55	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]

BLAST of SMED30023450 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5


Match Name	E-value	Identity	Description
ferd3l	6.187e-21	66.10	Fer3 like bHLH transcription factor [Source:NCBI g...	[more]
si:dkey-34f9.3	4.613e-19	63.49	si:dkey-34f9.3 [Source:ZFIN;Acc:ZDB-GENE-081104-41...	[more]
ptf1a	3.318e-16	60.78	pancreas associated transcription factor 1a [Sourc...	[more]
atoh7	1.212e-11	50.85	atonal bHLH transcription factor 7 [Source:NCBI ge...	[more]
atoh1b	1.406e-10	48.28	neurogenin-2 [Source:NCBI gene;Acc:101175327]	[more]

BLAST of SMED30023450 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5


Match Name	E-value	Identity	Description
SMESG000062901.1	2.535e-72	99.18	SMESG000062901.1	[more]
SMESG000029000.1	1.479e-21	68.85	SMESG000029000.1	[more]
SMESG000029000.1	1.937e-21	68.85	SMESG000029000.1	[more]
SMESG000019274.1	1.458e-18	65.63	SMESG000019274.1	[more]
SMESG000044960.1	5.584e-17	57.14	SMESG000044960.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence
protein sequence

transcript sequence

>SMED30023450 ID=SMED30023450|Name=SMED30023450|organism=Schmidtea mediterranea sexual|type=transcript|length=489bp
TTTTTTTTGATGAAGGGTAATAATTTATTTATCAAATTTTATCAAATTAA
TTTGTAATTTTTGGCCAAACCCCATTCAGAATGCATTCCATGAACTTTAT
ATACGCAATTGCTAGTCGTAACGTTTCTATTCTTGATAAACGTTTTTCAA
AGACAAATGTTGGAACTTTCAGTCGGAGATTTTCAAATGCCGCATTAAGA
TTAATCATTCGTTTTCTTTCTCTGATATTTGCTGCTGTTCTATGAGCAGT
CGATTTGTTTCTTCTTCGTTTTTGTTTTTGATATTTTTTACATTTTGTTG
AATCGTATTTTTCCAGTTCCTTAACGGTTGAAAAATGGCTGGGGTTTCTG
TCTAATAATTTAGCCGTTGTTATTGAAAAGTCAGATTTAAGGTCTAGCAT
AGTAATCAAGGCAATGAAAAAACATTTTCTTTTATAAGATTTGGGATATC
AGCCATTGATACATGCTTGAATGAATTTTATTGTCTTTC

protein sequence of SMED30023450-orf-1

>SMED30023450-orf-1 ID=SMED30023450-orf-1|Name=SMED30023450-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=118bp
MLDLKSDFSITTAKLLDRNPSHFSTVKELEKYDSTKCKKYQKQKRRRNKS
TAHRTAANIRERKRMINLNAAFENLRLKVPTFVFEKRLSRIETLRLAIAY
IKFMECILNGVWPKITN*

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0000418	head
PLANA:0000484	secretory system
PLANA:0002142	parenchyma

Vocabulary: **INTERPRO**
Term	Definition
IPR036638	HLH_DNA-bd_sf
IPR011598	bHLH_dom

Vocabulary: **molecular function**
Term	Definition
GO:0046983	protein dimerization activity

InterPro

Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain	SMART	SM00353	finulus	coord: 58..110 e-value: 5.2E-14 score: 62.6
IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain	PFAM	PF00010	HLH	coord: 54..104 e-value: 1.9E-15 score: 56.5
IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain	PROSITE	PS50888	BHLH	coord: 52..104 score: 14.43
IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain	CDD	cd00083	HLH	coord: 54..108 e-value: 1.86974E-12 score: 55.6831
IPR036638	Helix-loop-helix DNA-binding domain superfamily	GENE3D	G3DSA:4.10.280.10		coord: 52..110 e-value: 6.0E-20 score: 72.8
IPR036638	Helix-loop-helix DNA-binding domain superfamily	SUPERFAMILY	SSF47459	HLH, helix-loop-helix DNA-binding domain	coord: 49..110
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 38..53
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 37..56
None	No IPR available	PANTHER	PTHR23349:SF63	FER3-LIKE PROTEIN	coord: 34..110
None	No IPR available	PANTHER	PTHR23349	BASIC HELIX-LOOP-HELIX TRANSCRIPTION FACTOR, TWIST	coord: 34..110

Main menu

SMED30023450

Zeng et. al., 2018

▻ Overview

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

Explore this single cell expression dataset with our NB Cluster Shiny App

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Davies et. al., 2017

PAGE et. al., 2020

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 4

Owlery

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