SMED30023444

Overview


Name	SMED30023444
Smed ID	SMED30023444
Length (bp)	390

Neoblast Clusters

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.

Expression of SMED30023444 (SMED30023444) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30023444 (SMED30023444) in cells (dots) of each of the 12 neoblast clusters.

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30023444 (SMED30023444) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30023444 (SMED30023444) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30023444

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 1

Expressed In	Reference Transcript	Gene Models	Published Transcript	Transcriptome	Publication	Specimen	Lifecycle	Evidence
gut	SMED30023444	SMESG000049359.1	dd_Smed_v4_1815_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018

Note: Hover over icons to view figure legend

Alignments

SMED30023444 aligns in the following genomic locations:


Alignment Location	Alignment Score
v31.032664:418..902 -	1919
v31.001161:61468..61952 -	1901

Homology

[Close]

BLAST of SMED30023444 vs. Ensembl Human
Match: NDUFA2 (NADH:ubiquinone oxidoreductase subunit A2 [Source:HGNC Symbol;Acc:HGNC:7685])

HSP 1 Score: 90.8929 bits (224), Expect = 2.081e-24
Identity = 39/78 (50.00%), Postives = 56/78 (71.79%), Query Frame = -3

Query:   50 VRELRFHLCQKSEESKELRKFIELYYIAMKQSNPTLPILIRECSDTQPALYVRYEYGIERKIPLVSKSYENIFKDLEH 283
            +RE+R HLCQ+S  S+ +R FIE  Y+ +K++NP LPILIRECSD QP L+ RY +G E  +PL + S + + + LE+
Sbjct:   16 LREIRIHLCQRSPGSQGVRDFIEKRYVELKKANPDLPILIRECSDVQPKLWARYAFGQETNVPLNNFSADQVTRALEN 93

[Close]

BLAST of SMED30023444 vs. Ensembl Human
Match: NDUFA2 (NADH:ubiquinone oxidoreductase subunit A2 [Source:HGNC Symbol;Acc:HGNC:7685])

HSP 1 Score: 76.2554 bits (186), Expect = 5.931e-19
Identity = 32/54 (59.26%), Postives = 42/54 (77.78%), Query Frame = -3

Query:  122 VRELRFHLCQKSEESKELRKFIELYYIAMKQSNPTLPILIRECSDTQPALYVRY 283
            +RE+R HLCQ+S  S+ +R FIE  Y+ +K++NP LPILIRECSD QP L+ RY
Sbjct:   16 LREIRIHLCQRSPGSQGVRDFIEKRYVELKKANPDLPILIRECSDVQPKLWARY 69

[Close]

BLAST of SMED30023444 vs. Ensembl Celegans
Match: Y63D3A.7 (pep chromosome:WBcel235:I:14113636:14114085:1 gene:WBGene00013406.1 transcript:Y63D3A.7.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:Y63D3A.7)

HSP 1 Score: 80.1073 bits (196), Expect = 1.139e-20
Identity = 37/85 (43.53%), Postives = 52/85 (61.18%), Query Frame = -3

Query:   38 GKHVRELRFHLCQKSEESKELRKFIELYYIAMKQSNPTLPILIRECSDTQPALYVRYEYGIERKIPLVSKSYENIFKDLEHADAQ 292
            G  +RE+R H+CQKS  S  +R FIE  Y+ +K++NP  PILIRE S   P ++ RYE+G+ER   L   S EN+   +    +Q
Sbjct:    7 GTALREIRIHVCQKSPASAGVRAFIENDYVGIKKANPQFPILIREASGIVPRVFARYEHGVERVATLEDLSRENVKSTITQLASQ 91

[Close]

BLAST of SMED30023444 vs. Ensembl Fly
Match: ND-B8 (gene:FBgn0040705 transcript:FBtr0077440)

HSP 1 Score: 73.9442 bits (180), Expect = 3.766e-18
Identity = 36/81 (44.44%), Postives = 52/81 (64.20%), Query Frame = -3

Query:   53 FGKHVRELRFHLCQKSEESKELRKFIELYYIAMKQSNPTLPILIRECSDTQPALYVRYEYGIERKIPLVSKSYENIFKDLE 295
            F   ++ELR  L  K + SK +R+++E +Y  +K+SNP LPIL+RECS  QP LY RY  G E  + L + +  +I K+LE
Sbjct:   11 FTPKLKELRIILDPKGDTSKGVREYVERFYPNLKKSNPDLPILVRECSGVQPRLYARYGNGKEVSLSLANHAAPDIHKNLE 91

[Close]

BLAST of SMED30023444 vs. Ensembl Zebrafish
Match: ndufa2 (NADH:ubiquinone oxidoreductase subunit A2 [Source:ZFIN;Acc:ZDB-GENE-050522-377])

HSP 1 Score: 89.3521 bits (220), Expect = 7.167e-24
Identity = 39/82 (47.56%), Postives = 53/82 (64.63%), Query Frame = -3

Query:   53 KFGKHVRELRFHLCQKSEESKELRKFIELYYIAMKQSNPTLPILIRECSDTQPALYVRYEYGIERKIPLVSKSYENIFKDLE 298
               K++RE+R HLCQ S  S+  R FIE +Y+ +K++NP  PILIRECS  QP L+ RY +G E  + L + S E + K LE
Sbjct:   13 NLSKNLREIRLHLCQISAASQGTRDFIEQHYVTLKKANPQFPILIRECSGVQPVLWARYGFGKEHSVSLDNMSAEQVAKALE 94

[Close]

BLAST of SMED30023444 vs. Ensembl Xenopus
Match: glcci1 (glucocorticoid induced 1 [Source:Xenbase;Acc:XB-GENE-5843690])

HSP 1 Score: 88.1965 bits (217), Expect = 2.106e-23
Identity = 37/82 (45.12%), Postives = 56/82 (68.29%), Query Frame = -3

Query:   53 KFGKHVRELRFHLCQKSEESKELRKFIELYYIAMKQSNPTLPILIRECSDTQPALYVRYEYGIERKIPLVSKSYENIFKDLE 298
            K  ++VRE+R HLCQ S  S+ +R FIE +Y+ +K++NP  PILIRECS  QP L+ RY++G E  + L + + + + + LE
Sbjct:   12 KLSRNVREIRIHLCQSSAASQGVRDFIEQHYVELKKANPEFPILIRECSGVQPKLWARYDFGKETNVSLNNLNVDQVARALE 93

[Close]

BLAST of SMED30023444 vs. Ensembl Xenopus
Match: ENSXETT00000002810.1 (pep primary_assembly:Xenopus_tropicalis_v9.1:3:16018551:16020354:1 gene:ENSXETG00000006509.1 transcript:ENSXETT00000002810.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 61.2326 bits (147), Expect = 1.006e-12
Identity = 30/76 (39.47%), Postives = 44/76 (57.89%), Query Frame = -3

Query:   53 RELRFHLCQKSEESKELRKFIELYYIAMKQSNPTLPILIRECSDTQPALYVRYEYGIERKIPLVSKSYENIFKDLE 280
            REL   LC           FIE +Y+ +K++NP  PILIRECS  QP L+ RY++G E  + L + + + + + LE
Sbjct:   22 RELLLCLCGSPPLFTCECDFIEQHYVELKKANPEFPILIRECSGVQPKLWARYDFGKETNVSLNNLNVDQVARALE 97

[Close]

BLAST of SMED30023444 vs. Ensembl Xenopus
Match: gpr83.2 (G protein-coupled receptor 83 [Source:Xenbase;Acc:XB-GENE-993677])

HSP 1 Score: 61.2326 bits (147), Expect = 1.006e-12
Identity = 30/76 (39.47%), Postives = 44/76 (57.89%), Query Frame = -3

Query:   53 RELRFHLCQKSEESKELRKFIELYYIAMKQSNPTLPILIRECSDTQPALYVRYEYGIERKIPLVSKSYENIFKDLE 280
            REL   LC           FIE +Y+ +K++NP  PILIRECS  QP L+ RY++G E  + L + + + + + LE
Sbjct:   22 RELLLCLCGSPPLFTCECDFIEQHYVELKKANPEFPILIRECSGVQPKLWARYDFGKETNVSLNNLNVDQVARALE 97

[Close]

BLAST of SMED30023444 vs. Ensembl Mouse
Match: Ndufa2 (NADH:ubiquinone oxidoreductase subunit A2 [Source:MGI Symbol;Acc:MGI:1343103])

HSP 1 Score: 78.9518 bits (193), Expect = 7.814e-20
Identity = 33/78 (42.31%), Postives = 55/78 (70.51%), Query Frame = -3

Query:   50 VRELRFHLCQKSEESKELRKFIELYYIAMKQSNPTLPILIRECSDTQPALYVRYEYGIERKIPLVSKSYENIFKDLEH 283
            +RE+R HLCQ+S  S+ +R FI   Y+ +K+++P LPILIRECS+ QP L+ RY +G E+ + L + S + + + +++
Sbjct:   16 LREIRVHLCQRSPGSQGVRDFIVQRYVELKKAHPNLPILIRECSEVQPKLWARYAFGQEKTVSLNNLSADEVTRAMQN 93

[Close]

BLAST of SMED30023444 vs. UniProt/SwissProt
Match: sp|Q4R5E2|NDUA2_MACFA (NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 OS=Macaca fascicularis OX=9541 GN=NDUFA2 PE=3 SV=3)

HSP 1 Score: 92.0485 bits (227), Expect = 4.320e-24
Identity = 39/78 (50.00%), Postives = 57/78 (73.08%), Query Frame = -3

Query:   50 VRELRFHLCQKSEESKELRKFIELYYIAMKQSNPTLPILIRECSDTQPALYVRYEYGIERKIPLVSKSYENIFKDLEH 283
            +RE+R HLCQ+S  S+ +R FIE  Y+ +K++NP LPILIRECSD QP L+ RY +G E+ +PL + S + + + LE+
Sbjct:   16 LREIRIHLCQRSPGSQGVRDFIEKRYVELKKANPDLPILIRECSDVQPKLWARYAFGQEKNVPLNNFSADQVTRALEN 93

[Close]

BLAST of SMED30023444 vs. UniProt/SwissProt
Match: sp|P0CB80|NDUA2_PONPY (NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 OS=Pongo pygmaeus OX=9600 GN=NDUFA2 PE=3 SV=1)

HSP 1 Score: 91.6633 bits (226), Expect = 6.338e-24
Identity = 39/78 (50.00%), Postives = 57/78 (73.08%), Query Frame = -3

Query:   50 VRELRFHLCQKSEESKELRKFIELYYIAMKQSNPTLPILIRECSDTQPALYVRYEYGIERKIPLVSKSYENIFKDLEH 283
            +RE+R HLCQ+S  S+ +R FIE  Y+ +K++NP LPILIRECSD QP L+ RY +G E+ +PL + S + + + LE+
Sbjct:   16 LREIRIHLCQRSPGSQGVRDFIEKRYVELKKANPDLPILIRECSDVQPKLWARYAFGQEKNVPLNNFSADQVTRALEN 93

[Close]

BLAST of SMED30023444 vs. UniProt/SwissProt
Match: sp|P0CB79|NDUA2_PONAB (NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 OS=Pongo abelii OX=9601 GN=NDUFA2 PE=3 SV=1)

HSP 1 Score: 91.6633 bits (226), Expect = 6.338e-24
Identity = 39/78 (50.00%), Postives = 57/78 (73.08%), Query Frame = -3

Query:   50 VRELRFHLCQKSEESKELRKFIELYYIAMKQSNPTLPILIRECSDTQPALYVRYEYGIERKIPLVSKSYENIFKDLEH 283
            +RE+R HLCQ+S  S+ +R FIE  Y+ +K++NP LPILIRECSD QP L+ RY +G E+ +PL + S + + + LE+
Sbjct:   16 LREIRIHLCQRSPGSQGVRDFIEKRYVELKKANPDLPILIRECSDVQPKLWARYAFGQEKNVPLNNFSADQVTRALEN 93

[Close]

BLAST of SMED30023444 vs. UniProt/SwissProt
Match: sp|O43678|NDUA2_HUMAN (NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 OS=Homo sapiens OX=9606 GN=NDUFA2 PE=1 SV=3)

HSP 1 Score: 90.8929 bits (224), Expect = 9.995e-24
Identity = 39/78 (50.00%), Postives = 56/78 (71.79%), Query Frame = -3

Query:   50 VRELRFHLCQKSEESKELRKFIELYYIAMKQSNPTLPILIRECSDTQPALYVRYEYGIERKIPLVSKSYENIFKDLEH 283
            +RE+R HLCQ+S  S+ +R FIE  Y+ +K++NP LPILIRECSD QP L+ RY +G E  +PL + S + + + LE+
Sbjct:   16 LREIRIHLCQRSPGSQGVRDFIEKRYVELKKANPDLPILIRECSDVQPKLWARYAFGQETNVPLNNFSADQVTRALEN 93

[Close]

BLAST of SMED30023444 vs. UniProt/SwissProt
Match: sp|Q02370|NDUA2_BOVIN (NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 OS=Bos taurus OX=9913 GN=NDUFA2 PE=1 SV=2)

HSP 1 Score: 88.5817 bits (218), Expect = 9.019e-23
Identity = 38/78 (48.72%), Postives = 56/78 (71.79%), Query Frame = -3

Query:   50 VRELRFHLCQKSEESKELRKFIELYYIAMKQSNPTLPILIRECSDTQPALYVRYEYGIERKIPLVSKSYENIFKDLEH 283
            +RE+R HLCQ+S  S+ +R FIE  Y+ +K++NP LPILIRECSD QP L+ RY +G E+ + L + S + + + LE+
Sbjct:   16 LREIRIHLCQRSPGSQGVRDFIEKRYVELKKANPDLPILIRECSDVQPKLWARYAFGQEKNVSLNNFSADQVTRALEN 93

[Close]

BLAST of SMED30023444 vs. TrEMBL
Match: A0A4D5R8T7 (NADH dehydrogenase (Ubiquinone) 1 alpha subcomplex subunit 2 OS=Scolopendra viridis OX=118503 PE=4 SV=1)

HSP 1 Score: 103.219 bits (256), Expect = 4.446e-26
Identity = 45/88 (51.14%), Postives = 63/88 (71.59%), Query Frame = -3

Query:   38 LKFGKHVRELRFHLCQKSEESKELRKFIELYYIAMKQSNPTLPILIRECSDTQPALYVRYEYGIERKIPLVSKSYENIFKDLEHADAQ 301
            +K G H+RELR HLCQKS+ S+ +R+FIE YY+ +K++NP  PILIRECS   P +Y RYE+G E+ IPL   S + + K++E   A+
Sbjct:    7 IKLGPHLRELRIHLCQKSDASRGVREFIEKYYVPLKKNNPRFPILIRECSGVLPKIYARYEFGEEKNIPLSGLSSDEVLKNIEKLSAK 94

[Close]

BLAST of SMED30023444 vs. TrEMBL
Match: A0A2W1BYA8 (L51_S25_CI-B8 domain-containing protein OS=Helicoverpa armigera OX=29058 GN=HaOG203120 PE=4 SV=1)

HSP 1 Score: 101.679 bits (252), Expect = 1.390e-25
Identity = 40/84 (47.62%), Postives = 63/84 (75.00%), Query Frame = -3

Query:   50 LKFGKHVRELRFHLCQKSEESKELRKFIELYYIAMKQSNPTLPILIRECSDTQPALYVRYEYGIERKIPLVSKSYENIFKDLEH 301
            ++ G H+RE+R HLCQ S++S+ +RKFI+ +Y+++K+ NP+ PILIRECS  QP ++ RYE G+ER +PL  ++ EN+   L+ 
Sbjct:    3 VRLGGHLREIRIHLCQTSKQSEGVRKFIQKHYVSIKKENPSFPILIRECSGVQPRIWARYEKGVERSMPLTDQTDENVLSALKQ 86

[Close]

BLAST of SMED30023444 vs. TrEMBL
Match: J9K680 (L51_S25_CI-B8 domain-containing protein OS=Acyrthosiphon pisum OX=7029 GN=100167589 PE=4 SV=1)

HSP 1 Score: 101.679 bits (252), Expect = 1.488e-25
Identity = 44/85 (51.76%), Postives = 61/85 (71.76%), Query Frame = -3

Query:   53 SFLKFGKHVRELRFHLCQKSEESKELRKFIELYYIAMKQSNPTLPILIRECSDTQPALYVRYEYGIERKIPLVSKSYENIFKDLE 307
            S +KFG H++ELR HLCQ S  SK +R+FIE YYI MK++NP  PIL+RECS  +P ++ R+E+G ER   L  +S +N+ K +E
Sbjct:    2 SAIKFGSHLKELRLHLCQSSSSSKGVRQFIEKYYIPMKKANPKFPILVRECSGVEPKVFARFEFGQERSEQLSDQSADNVLKKIE 86

[Close]

BLAST of SMED30023444 vs. TrEMBL
Match: Q4PM50 (NADH dehydrogenase 1 alpha subcomplex-like OS=Ixodes scapularis OX=6945 GN=8031708 PE=2 SV=1)

HSP 1 Score: 100.908 bits (250), Expect = 3.358e-25
Identity = 42/81 (51.85%), Postives = 56/81 (69.14%), Query Frame = -3

Query:   56 KFGKHVRELRFHLCQKSEESKELRKFIELYYIAMKQSNPTLPILIRECSDTQPALYVRYEYGIERKIPLVSKSYENIFKDL 298
            KFG H++ELR HLCQKS ES  +R+FI  +Y ++K SNPTLP+LIRECS  QP LY RY +G E  +PL   + + +   +
Sbjct:    9 KFGAHLKELRLHLCQKSSESAGVREFITKHYPSLKASNPTLPVLIRECSGVQPKLYARYAFGKESHVPLAGMTADQVLAAV 89

[Close]

BLAST of SMED30023444 vs. TrEMBL
Match: A0A1S3JAG4 (NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 OS=Lingula unguis OX=7574 GN=LOC106171487 PE=4 SV=1)

HSP 1 Score: 100.908 bits (250), Expect = 3.388e-25
Identity = 43/81 (53.09%), Postives = 60/81 (74.07%), Query Frame = -3

Query:   56 KFGKHVRELRFHLCQKSEESKELRKFIELYYIAMKQSNPTLPILIRECSDTQPALYVRYEYGIERKIPLVSKSYENIFKDL 298
            KFG H++ELR HLCQKS +S+ +R+FIE +Y+ +KQ+NP  PILIREC   QP LY RYE+G E  + L SK+ + I +++
Sbjct:    8 KFGNHLKELRIHLCQKSPQSQGVREFIENHYVPLKQANPRFPILIRECQGVQPVLYARYEFGKESSMSLASKTKDQILQEI 88

[Close]

BLAST of SMED30023444 vs. Ensembl Cavefish
Match: ndufa2 (NADH:ubiquinone oxidoreductase subunit A2 [Source:NCBI gene;Acc:103046306])

HSP 1 Score: 86.2705 bits (212), Expect = 6.989e-23
Identity = 37/83 (44.58%), Postives = 54/83 (65.06%), Query Frame = -3

Query:   47 FGKHVRELRFHLCQKSEESKELRKFIELYYIAMKQSNPTLPILIRECSDTQPALYVRYEYGIERKIPLVSKSYENIFKDLEHA 295
              +++RE+R H CQ S  S+  R+F+E +Y+ +K++NP  PILIRECS  QP L+ RY  G ER   L + + E + K+LE A
Sbjct:   11 LARNLREIRLHFCQSSPASQGAREFVEQFYVPLKKANPEFPILIRECSGVQPKLWARYGLGKERSFALDNMNAEQVAKELEKA 93

[Close]

BLAST of SMED30023444 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000006311.1 (pep scaffold:Pmarinus_7.0:GL479573:20443:22276:1 gene:ENSPMAG00000005704.1 transcript:ENSPMAT00000006311.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 82.4185 bits (202), Expect = 4.682e-22
Identity = 39/86 (45.35%), Postives = 55/86 (63.95%), Query Frame = -3

Query:   53 SFLKFGK-HVRELRFHLCQKSEESKELRKFIELYYIAMKQSNPTLPILIRECSDTQPALYVRYEYGIERKIPLVSKSYENIFKDLE 307
            S L+F    +RELR HL Q SE S+ +R+FIE  ++ +K  NP LP+L+REC D    L+ RY YG ER + L + S E + K++E
Sbjct:    5 SSLRFASPALRELRLHLSQSSEASRGVREFIEARFVTLKTENPNLPVLVRECRDVPARLWARYAYGQERSVLLDNLSAEQVTKEVE 90

[Close]

BLAST of SMED30023444 vs. Ensembl Nematostella
Match: EDO44422 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RVP0])

HSP 1 Score: 87.4261 bits (215), Expect = 1.140e-23
Identity = 38/82 (46.34%), Postives = 53/82 (64.63%), Query Frame = -3

Query:   53 KFGKHVRELRFHLCQKSEESKELRKFIELYYIAMKQSNPTLPILIRECSDTQPALYVRYEYGIERKIPLVSKSYENIFKDLE 298
            + GK  +E+R HLCQKS  S+ +R FIE  Y+ +K++NP  PILIRECS  QP +Y R++YG E  + L     E + K +E
Sbjct:    8 QLGKFAKEIRIHLCQKSPSSQGVRSFIEKNYVDIKKANPKFPILIRECSGIQPKMYARFDYGKESSVSLQDMKSEEVMKAME 89

[Close]

BLAST of SMED30023444 vs. Planmine SMEST
Match: SMESG000049359.1 (SMESG000049359.1)

HSP 1 Score: 190.66 bits (483), Expect = 2.655e-64
Identity = 92/92 (100.00%), Postives = 92/92 (100.00%), Query Frame = -3

Query:   38 MASFLKFGKHVRELRFHLCQKSEESKELRKFIELYYIAMKQSNPTLPILIRECSDTQPALYVRYEYGIERKIPLVSKSYENIFKDLEHADAQ 313
            MASFLKFGKHVRELRFHLCQKSEESKELRKFIELYYIAMKQSNPTLPILIRECSDTQPALYVRYEYGIERKIPLVSKSYENIFKDLEHADAQ
Sbjct:    1 MASFLKFGKHVRELRFHLCQKSEESKELRKFIELYYIAMKQSNPTLPILIRECSDTQPALYVRYEYGIERKIPLVSKSYENIFKDLEHADAQ 92

The following BLAST results are available for this feature:

BLAST of SMED30023444 vs. Ensembl Human
BLAST of SMED30023444 vs. Ensembl Celegans
BLAST of SMED30023444 vs. Ensembl Fly
BLAST of SMED30023444 vs. Ensembl Zebrafish
BLAST of SMED30023444 vs. Ensembl Xenopus
BLAST of SMED30023444 vs. Ensembl Mouse
BLAST of SMED30023444 vs. UniProt/SwissProt
BLAST of SMED30023444 vs. TrEMBL
BLAST of SMED30023444 vs. Ensembl Cavefish
BLAST of SMED30023444 vs. Ensembl Sea Lamprey
BLAST of SMED30023444 vs. Ensembl Yeast
BLAST of SMED30023444 vs. Ensembl Nematostella
BLAST of SMED30023444 vs. Ensembl Medaka
BLAST of SMED30023444 vs. Planmine SMEST

BLAST of SMED30023444 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 2


Match Name	E-value	Identity	Description
NDUFA2	2.081e-24	50.00	NADH:ubiquinone oxidoreductase subunit A2 [Source:...	[more]
NDUFA2	5.931e-19	59.26	NADH:ubiquinone oxidoreductase subunit A2 [Source:...	[more]

BLAST of SMED30023444 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 1


Match Name	E-value	Identity	Description
Y63D3A.7	1.139e-20	43.53	pep chromosome:WBcel235:I:14113636:14114085:1 gene...	[more]

BLAST of SMED30023444 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 1


Match Name	E-value	Identity	Description
ND-B8	3.766e-18	44.44	gene:FBgn0040705 transcript:FBtr0077440	[more]

BLAST of SMED30023444 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1


Match Name	E-value	Identity	Description
ndufa2	7.167e-24	47.56	NADH:ubiquinone oxidoreductase subunit A2 [Source:...	[more]

BLAST of SMED30023444 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 3


Match Name	E-value	Identity	Description
glcci1	2.106e-23	45.12	glucocorticoid induced 1 [Source:Xenbase;Acc:XB-GE...	[more]
ENSXETT00000002810.1	1.006e-12	39.47	pep primary_assembly:Xenopus_tropicalis_v9.1:3:160...	[more]
gpr83.2	1.006e-12	39.47	G protein-coupled receptor 83 [Source:Xenbase;Acc:...	[more]

BLAST of SMED30023444 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 1


Match Name	E-value	Identity	Description
Ndufa2	7.814e-20	42.31	NADH:ubiquinone oxidoreductase subunit A2 [Source:...	[more]

BLAST of SMED30023444 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5


Match Name	E-value	Identity	Description
sp\|Q4R5E2\|NDUA2_MACFA	4.320e-24	50.00	NADH dehydrogenase [ubiquinone] 1 alpha subcomplex...	[more]
sp\|P0CB80\|NDUA2_PONPY	6.338e-24	50.00	NADH dehydrogenase [ubiquinone] 1 alpha subcomplex...	[more]
sp\|P0CB79\|NDUA2_PONAB	6.338e-24	50.00	NADH dehydrogenase [ubiquinone] 1 alpha subcomplex...	[more]
sp\|O43678\|NDUA2_HUMAN	9.995e-24	50.00	NADH dehydrogenase [ubiquinone] 1 alpha subcomplex...	[more]
sp\|Q02370\|NDUA2_BOVIN	9.019e-23	48.72	NADH dehydrogenase [ubiquinone] 1 alpha subcomplex...	[more]

BLAST of SMED30023444 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A4D5R8T7	4.446e-26	51.14	NADH dehydrogenase (Ubiquinone) 1 alpha subcomplex...	[more]
A0A2W1BYA8	1.390e-25	47.62	L51_S25_CI-B8 domain-containing protein OS=Helicov...	[more]
J9K680	1.488e-25	51.76	L51_S25_CI-B8 domain-containing protein OS=Acyrtho...	[more]
Q4PM50	3.358e-25	51.85	NADH dehydrogenase 1 alpha subcomplex-like OS=Ixod...	[more]
A0A1S3JAG4	3.388e-25	53.09	NADH dehydrogenase [ubiquinone] 1 alpha subcomplex...	[more]

BLAST of SMED30023444 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1


Match Name	E-value	Identity	Description
ndufa2	6.989e-23	44.58	NADH:ubiquinone oxidoreductase subunit A2 [Source:...	[more]

BLAST of SMED30023444 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1


Match Name	E-value	Identity	Description
ENSPMAT00000006311.1	4.682e-22	45.35	pep scaffold:Pmarinus_7.0:GL479573:20443:22276:1 g...	[more]

BLAST of SMED30023444 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30023444 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1


Match Name	E-value	Identity	Description
EDO44422	1.140e-23	46.34	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]

BLAST of SMED30023444 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30023444 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1


Match Name	E-value	Identity	Description
SMESG000049359.1	2.655e-64	100.00	SMESG000049359.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence

transcript sequence

>SMED30023444 ID=SMED30023444|Name=SMED30023444|organism=Schmidtea mediterranea sexual|type=transcript|length=390bp
TAAGTGACTAAAATTTATTTCAACTAAATTAAATTTATTGTGCATCTGCA
TGCTCTAAATCCTTGAAAATATTTTCGTAAGATTTAGAAACTAGTGGAAT
TTTTCTTTCTATACCATATTCATATCTTACATAGAGTGCTGGCTGAGTGT
CCGAACACTCTCTAATTAAAATGGGAAGAGTAGGATTACTCTGTTTCATA
GCAATATAATATAATTCAATGAATTTTCTTAATTCCTTACTTTCCTCTGA
TTTTTGACATAAATGAAATCTTAATTCACGAACATGTTTACCAAATTTTA
AAAAAGATGCCATTCAAATAATTAGGTTAAATTTTTAAATAGTTTGACGT
ACTATAACAAGAAAATATTTAAAAGTTTTCTTTACTATTT

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0000026	gut

Vocabulary: **INTERPRO**
Term	Definition
IPR036249	Thioredoxin-like_sf
IPR016464	NADH_Ub_cplx-1_asu_su-2
IPR007741	Ribosome/NADH_DH
IPR012336	Thioredoxin-like_fold

InterPro

Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR007741	Ribosomal protein/NADH dehydrogenase domain	SMART	SM00916	L51_S25_CI_B8_2	coord: 20..92 e-value: 1.3E-16 score: 71.1
IPR007741	Ribosomal protein/NADH dehydrogenase domain	PFAM	PF05047	L51_S25_CI-B8	coord: 28..78 e-value: 1.8E-11 score: 43.8
IPR016464	NADH dehydrogenase [ubiquinone] (complex I), alpha subcomplex, subunit 2	PIRSF	PIRSF005822	NDUA2	coord: 1..92 e-value: 1.3E-29 score: 100.3
IPR016464	NADH dehydrogenase [ubiquinone] (complex I), alpha subcomplex, subunit 2	PANTHER	PTHR12878	NADH-UBIQUINONE OXIDOREDUCTASE B8 SUBUNIT	coord: 2..88
IPR036249	Thioredoxin-like superfamily	GENE3D	G3DSA:3.40.30.10		coord: 1..91 e-value: 1.8E-29 score: 103.8
IPR036249	Thioredoxin-like superfamily	SUPERFAMILY	SSF52833	Thioredoxin-like	coord: 10..87

Main menu

SMED30023444

Zeng et. al., 2018

▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Davies et. al., 2017

PAGE et. al., 2020

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 1

Owlery

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