FMRFamide-activated amiloride-sensitive sodium channel

Overview
NameFMRFamide-activated amiloride-sensitive sodium channel
Smed IDSMED30023439
Length (bp)891
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of FMRFamide-activated amiloride-sensitive sodium channel (SMED30023439) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for FMRFamide-activated amiloride-sensitive sodium channel (SMED30023439) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30023439

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 5

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
nervous systemSMED30023439h1SMcG0016111 dd_Smed_v4_13880_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30023439h1SMcG0016111 dd_Smed_v4_13880_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymaSMED30023439h1SMcG0016111 dd_Smed_v4_13880_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30023439h1SMcG0016111 dd_Smed_v4_13880_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
anterior sideSMED30023439h1SMcG0016111 dd_Smed_v4_13880_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of FMRFamide-activated amiloride-sensitive sodium channel vs. Ensembl Celegans
Match: asic-2 (Degenerin-like protein asic-2 [Source:UniProtKB/Swiss-Prot;Acc:Q22851])

HSP 1 Score: 48.9062 bits (115), Expect = 2.789e-6
Identity = 27/82 (32.93%), Postives = 38/82 (46.34%), Query Frame = 3 
Query:  255 HFCKNTTIRGVPRIVNTTNKSSKILWISFFIFLCAGLTICLTFLIRQFIEYSVIHPPRVLQNSPSPFPSFTVCNHRPLSYNI 500
             F   +T+  VP + N  NK S+I WI+ F+F+       L  LI +F  Y       +L      FP  TVCN  P  Y++
Sbjct:   12 DFSNWSTVAVVPHVANANNKISRIFWIAIFLFVLGMFAYELYILIAKFFSYPATVNTEILFEK-QIFPVVTVCNMNPYKYSV 92
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BLAST of FMRFamide-activated amiloride-sensitive sodium channel vs. TrEMBL
Match: A0A1S8WV40 (Uncharacterized protein OS=Opisthorchis viverrini OX=6198 GN=X801_05956 PE=3 SV=1)

HSP 1 Score: 166.007 bits (419), Expect = 9.238e-47
Identity = 81/185 (43.78%), Postives = 118/185 (63.78%), Query Frame = 3 
Query:  192 IQEEAILNSPTFKKL------LKNELLHFCKNTTIRGVPRIVNTTNKSSKILWISFFIFLCAGLTICLTFLIRQFIEYSVIHPPRVLQNSPSPFPSFTVCNHRPLSYNISSFFNQRNMRNPREFAIAANYVSKKYLFDRGDKGHYSLITSSFSLSGYLESIPLEYHNMLGHDYYDFVIDCLLINT 728
            IQ E    +PT +++      +K EL+ FC+ TTIRGV R+VN  +KS +I+W S  +FL  GL IC+ FL RQ++EY VIHPP+VL+++PSPFPS T+CN RPL+   +    +  ++ PR+FA+  N  + +  + +GD   Y LITS+ S+ GYLES+P E    LGH     +I C+  +T
Sbjct:   15 IQYEESHTNPTNRQIKPIRTKIKKELILFCQTTTIRGVTRVVNARSKSFQIVWASSVLFLFIGLFICMFFLTRQYLEYHVIHPPQVLRDTPSPFPSITLCNLRPLASEATKVLEESRIKTPRKFAMDVNSFAAEAFYHKGDVHSYQLITSAISMGGYLESLPPEIRLQLGHHMNQTIIHCMYYHT 199
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BLAST of FMRFamide-activated amiloride-sensitive sodium channel vs. TrEMBL
Match: A0A074Z6V3 (Uncharacterized protein OS=Opisthorchis viverrini OX=6198 GN=T265_10352 PE=3 SV=1)

HSP 1 Score: 165.236 bits (417), Expect = 1.772e-46
Identity = 80/182 (43.96%), Postives = 117/182 (64.29%), Query Frame = 3 
Query:  192 IQEEAILNSPTFKKL------LKNELLHFCKNTTIRGVPRIVNTTNKSSKILWISFFIFLCAGLTICLTFLIRQFIEYSVIHPPRVLQNSPSPFPSFTVCNHRPLSYNISSFFNQRNMRNPREFAIAANYVSKKYLFDRGDKGHYSLITSSFSLSGYLESIPLEYHNMLGHDYYDFVIDCLL 719
            IQ E    +PT +++      +K EL+ FC+ TTIRGV R+VN  +KS +I+W S  +FL  GL IC+ FL RQ++EY VIHPP+VL+++PSPFPS T+CN RPL+   +    +  ++ PR+FA+  N  + +  + +GD   Y LITS+ S+ GYLES+P E    LGH     +I C++
Sbjct:   15 IQYEESHTNPTNRQIKPIRTKIKKELILFCQTTTIRGVTRVVNARSKSFQIVWASSVLFLFIGLFICMFFLTRQYLEYHVIHPPQVLRDTPSPFPSITLCNLRPLASEATKVLEESRIKTPRKFAMDVNSFAAEAFYHKGDVHSYQLITSAISMGGYLESLPPEIRLQLGHHMNQTIIHCMV 196
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BLAST of FMRFamide-activated amiloride-sensitive sodium channel vs. TrEMBL
Match: A0A095C344 (Uncharacterized protein OS=Schistosoma haematobium OX=6185 GN=MS3_04402 PE=3 SV=1)

HSP 1 Score: 165.622 bits (418), Expect = 2.689e-46
Identity = 78/170 (45.88%), Postives = 112/170 (65.88%), Query Frame = 3 
Query:  225 FKKLLKNELLHFCKNTTIRGVPRIVNTTNKSSKILWISFFIFLCAGLTICLTFLIRQFIEYSVIHPPRVLQNSPSPFPSFTVCNHRPLSYNISSFFNQRNMRNPREFAIAANYVSKKYLFDRGDKGHYSLITSSFSLSGYLESIPLEYHNMLGHDYYDFVIDCLLINTVR 734
             +K LK E LHFC+ TTIRGV R+V   NK+ +ILWI+  I    GL +C+  L RQ++EY+VIHPP+VL+++PSPFPS T+CN RPLS   +S  N+ N+++PR+FAI  N  + K  +   D   Y  +TS+ S+ GYLES+P      LGH+    VI C+++  ++
Sbjct:   16 LRKDLKKEFLHFCQTTTIRGVTRVVTARNKTLQILWIASVILFFGGLFVCMFILTRQYLEYNVIHPPQVLRDTPSPFPSLTLCNLRPLSSEANSILNKYNLKSPRQFAIDVNNFAAKAYYFSNDIHSYQQVTSAVSMGGYLESLPQIVVPKLGHNLSHTVIHCMVVEFIK 185
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BLAST of FMRFamide-activated amiloride-sensitive sodium channel vs. TrEMBL
Match: A0A183Q225 (Uncharacterized protein OS=Schistosoma mattheei OX=31246 GN=SMTD_LOCUS20661 PE=3 SV=1)

HSP 1 Score: 165.622 bits (418), Expect = 2.768e-46
Identity = 79/169 (46.75%), Postives = 110/169 (65.09%), Query Frame = 3 
Query:  213 NSPTFKKLLKNELLHFCKNTTIRGVPRIVNTTNKSSKILWISFFIFLCAGLTICLTFLIRQFIEYSVIHPPRVLQNSPSPFPSFTVCNHRPLSYNISSFFNQRNMRNPREFAIAANYVSKKYLFDRGDKGHYSLITSSFSLSGYLESIPLEYHNMLGHDYYDFVIDCLL 719
            N    +K LK E LHFC+ TTIRGV R+V   NK+ +ILWI+  I    GL +C+  L RQ++EY+VIHPP+VL+++PSPFPS T+CN RPLS   +S  N+ N+++PR+FAI  N  + K  +   D   Y  +TS+ S+ GYLES+P      LGH+    VI C++
Sbjct:   12 NESQLRKDLKKEFLHFCQTTTIRGVTRVVTARNKTLQILWIASVILFFGGLFVCMFILTRQYLEYNVIHPPQVLRDTPSPFPSLTLCNLRPLSSEANSILNKYNLKSPRQFAIDVNNFAAKAYYFSNDIHSYQQVTSAVSMGGYLESLPQIVVPKLGHNLSHTVIHCMV 180
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BLAST of FMRFamide-activated amiloride-sensitive sodium channel vs. TrEMBL
Match: A0A4Z2DB23 (FMRFamide-activated amiloride-sensitive sodium channel OS=Schistosoma japonicum OX=6182 GN=EWB00_002747 PE=3 SV=1)

HSP 1 Score: 172.94 bits (437), Expect = 3.164e-46
Identity = 84/189 (44.44%), Postives = 124/189 (65.61%), Query Frame = 3 
Query:  186 NQIQEEAILNSPTFKKLLKNELLHFCKNTTIRGVPRIVNTTNKSSKILWISFFIFLCAGLTICLTFLIRQFIEYSVIHPPRVLQNSPSPFPSFTVCNHRPLSYNISSFFNQRNMRNPREFAIAANYVSKKYLFDRGDKGHYSLITSSFSLSGYLESIPLEYHNMLGHDYYDFVIDCLLINTVRRQGSKQ 752
            N+ ++    N    +K LK E L+FC+ TTIRGV R+VN  N++ + LWI+  I    GL IC+  LI+Q++EY+VIHPP+VL+++PSPFPS T+CN RPLS   +S  N+ N+++PR+FAI  N  + K  +   DK  Y  +TS+ S+ GYLES+P    + LGH+    VI C++I+    +GSK+
Sbjct:    3 NKFKKSISTNESQLRKDLKKEFLYFCQTTTIRGVTRVVNARNQTLQTLWIASVILFFGGLFICMFILIKQYLEYNVIHPPQVLRDTPSPFPSLTLCNLRPLSSEANSILNKYNLKSPRQFAIDVNNFAAKAYYYSNDKHSYQQVTSAISMGGYLESLPQIVVSKLGHNLSHTVIHCMVIHA---EGSKR 188
[Close]
BLAST of FMRFamide-activated amiloride-sensitive sodium channel vs. Planmine SMEST
Match: SMESG000069611.1 (SMESG000069611.1)

HSP 1 Score: 442.965 bits (1138), Expect = 1.458e-153
Identity = 214/224 (95.54%), Postives = 216/224 (96.43%), Query Frame = 3 
Query:   87 MHGNNLLQEPQLLKNVCWFCKSSVKTTLEDFHSNQIQEEAILNSPTFKKLLKNELLHFCKNTTIRGVPRIVNTTNKSSKILWISFFIFLCAGLTICLTFLIRQFIEYSVIHPPRVLQNSPSPFPSFTVCNHRPLSYNISSFFNQRNMRNPREFAIAANYVSKKYLFDRGDKGHYSLITSSFSLSGYLESIPLEYHNMLGHDYYDFVIDCLLINTVRRQGSKQSI 758
            MHGNNLLQEPQLLKNVCWFCKSSVKTTLEDFHSNQIQEEAILNSPTFKKLLKNELLHFCKNTTIRGVPRIVNTTNKSSKILWISFFIFLCAGLTICLTFLIRQFIEYSVIHPPRVLQNSPSPFPSFTVCNHRPLSYNISSFFNQRNMRNPREFAIAANYVSKKYLFDRGDKGHYSLITSSFSLSGYLESIPLEYHNMLGHDYYDFVIDCL+     R GSK  +
Sbjct:    1 MHGNNLLQEPQLLKNVCWFCKSSVKTTLEDFHSNQIQEEAILNSPTFKKLLKNELLHFCKNTTIRGVPRIVNTTNKSSKILWISFFIFLCAGLTICLTFLIRQFIEYSVIHPPRVLQNSPSPFPSFTVCNHRPLSYNISSFFNQRNMRNPREFAIAANYVSKKYLFDRGDKGHYSLITSSFSLSGYLESIPLEYHNMLGHDYYDFVIDCLI---TFRNGSKHHV 221
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BLAST of FMRFamide-activated amiloride-sensitive sodium channel vs. Planmine SMEST
Match: SMESG000028687.1 (SMESG000028687.1)

HSP 1 Score: 164.466 bits (415), Expect = 3.984e-46
Identity = 72/162 (44.44%), Postives = 109/162 (67.28%), Query Frame = 3 
Query:  228 KKLLKNELLHFCKNTTIRGVPRIVNTTNKSSKILWISFFIFLCAGLTICLTFLIRQFIEYSVIHPPRVLQNSPSPFPSFTVCNHRPLSYNISSFFNQRNMRNPREFAIAANYVSKKYLFDRGDKGHYSLITSSFSLSGYLESIPLEYHNMLGHDYYDFVIDC 713
            ++++K E L FC+NTTIRGVP+I+ + +K  + +W++ FI L +G ++C  FL++Q+  Y VIHPPR+L NS +PFPSFTVCN RPL+ NI + F  +N++ PREFA   N  + KY +++ +   Y   +S   + GYLES+P + +  LGH   D +I C
Sbjct:   39 RQVIKEEFLRFCENTTIRGVPKIIKSKHKPLRYMWMTCFILLLSGFSVCFIFLLKQYFAYDVIHPPRILTNSQAPFPSFTVCNLRPLARNIDTIFKLKNLKTPREFAKNVNNYAIKYFYNKNNLTLYGHASSIMLMGGYLESVPNKSYVELGHKKQDLIIHC 200
[Close]
BLAST of FMRFamide-activated amiloride-sensitive sodium channel vs. Planmine SMEST
Match: SMESG000078360.1 (SMESG000078360.1)

HSP 1 Score: 163.696 bits (413), Expect = 1.286e-45
Identity = 81/182 (44.51%), Postives = 120/182 (65.93%), Query Frame = 3 
Query:  234 LLKNELLHFCKNTTIRGVPRIVNTTNKSSKILWISFFIFLCAGLTICLTFLIRQFIEYSVIHPPRVLQNSPSPFPSFTVCNHRPLSYNISSFFNQRNMRNPREFAIAAN-YVSKKYLFDRGDKGHYSLITSSFSLSGYLESIPLEYHNMLGHDYYDFVIDCLLINTVRRQGSKQSILNHNSL 776
            LLK+EL+ FC+NTT+RG+PRI+   +++ +ILWI+F + L  GL + + FL +Q++EY+VIHPP +L +   PFPS ++CN RPLS     F  + N RNPREF    N YV+ K+ +++  K +Y+  T++ S++GYLES+P +  + LGH   + VI CL+I  V   GSK  I    SL
Sbjct:   75 LLKHELIAFCQNTTMRGIPRIIKAQSRNLRILWITFVVILFIGLLLSMLFLTKQYLEYNVIHPPVILPDQEPPFPSISLCNLRPLSRFGLEFLKKNNWRNPREFGNNLNVYVANKWYYNQSTKHYYTQFTTAISMNGYLESVPFDVLDKLGHSLDELVIHCLVIYNV---GSKSEIQKCGSL 253
[Close]
BLAST of FMRFamide-activated amiloride-sensitive sodium channel vs. Planmine SMEST
Match: SMESG000044381.1 (SMESG000044381.1)

HSP 1 Score: 157.532 bits (397), Expect = 1.165e-43
Identity = 77/175 (44.00%), Postives = 112/175 (64.00%), Query Frame = 3 
Query:  189 QIQEEAILNSPTFKKLLKNELLHFCKNTTIRGVPRIVNTTNKSSKILWISFFIFLCAGLTICLTFLIRQFIEYSVIHPPRVLQNSPSPFPSFTVCNHRPLSYNISSFFNQRNMRNPREFAIAANYVSKKYLFDRGDKGHYSLITSSFSLSGYLESIPLEYHNMLGHDYYDFVIDC 713
            ++  E I+N   FK + K E L FC+NTT+RGVPRIV +     + LW++FFI L  G  +C+ FL  Q++EY+VIHPPR+L ++ +PFPS TVCN+RP+  NI     +RN+++PREFA + N  + KY + +    +Y   +S  ++  YLES+P    N LGHD  D +I C
Sbjct:    5 ELDSEIIIN---FKTISKEEFLRFCENTTLRGVPRIVKSRKSHIRHLWLTFFILLFIGFMVCIIFLCYQYLEYNVIHPPRILSDNKAPFPSLTVCNYRPMKRNILEIMKKRNIKDPREFAHSINKYAIKYYYKKNLGKNYQRASSIMNMGAYLESVPNRTFNELGHDEQDLIIYC 176
[Close]
BLAST of FMRFamide-activated amiloride-sensitive sodium channel vs. Planmine SMEST
Match: SMESG000037525.1 (SMESG000037525.1)

HSP 1 Score: 153.68 bits (387), Expect = 2.000e-42
Identity = 84/167 (50.30%), Postives = 108/167 (64.67%), Query Frame = 3 
Query:  219 PTFKKLLKNELLHFCKNTTIRGVPRIVNTTNKSSKILWISFFIFLCAGLTICLTFLIRQFIEYSVIHPPRVLQNSPSPFPSFTVCNHRPLSYNISSFFNQRNMRNPREFAIAAN-YVSKKYLFDRGDKGHYSLITSSFSLSGYLESIP-LEYHNMLGHDYYDFVIDC 713
            P   + +K E L FC+NTTIRGV RIV +T+K    LW+ FFI LC GL +C+  L+ Q+++Y+VIHPPRVL N  SPFPSFT+CN RPLS ++ SF     +RNPREFA   N Y   +YL  + D  HY+  T+  S+ GYLES+P   Y   LGH   + VI C
Sbjct:   64 PKRGECMKKEFLLFCQNTTIRGVSRIVKSTDKKIFFLWLGFFIILCIGLLVCMILLLLQYLDYNVIHPPRVLSNKESPFPSFTICNLRPLSPDVRSFLANEKLRNPREFASRLNQYAVSRYL-KKNDSFHYAASTAISSMGGYLESMPNTSYFKELGHGLDEMVIFC 229
The following BLAST results are available for this feature:
BLAST of FMRFamide-activated amiloride-sensitive sodium channel vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of FMRFamide-activated amiloride-sensitive sodium channel vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 1
Match NameE-valueIdentityDescription
asic-22.789e-632.93Degenerin-like protein asic-2 [Source:UniProtKB/S... [more]
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BLAST of FMRFamide-activated amiloride-sensitive sodium channel vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of FMRFamide-activated amiloride-sensitive sodium channel vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of FMRFamide-activated amiloride-sensitive sodium channel vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of FMRFamide-activated amiloride-sensitive sodium channel vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of FMRFamide-activated amiloride-sensitive sodium channel vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of FMRFamide-activated amiloride-sensitive sodium channel vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A1S8WV409.238e-4743.78Uncharacterized protein OS=Opisthorchis viverrini ... [more]
A0A074Z6V31.772e-4643.96Uncharacterized protein OS=Opisthorchis viverrini ... [more]
A0A095C3442.689e-4645.88Uncharacterized protein OS=Schistosoma haematobium... [more]
A0A183Q2252.768e-4646.75Uncharacterized protein OS=Schistosoma mattheei OX... [more]
A0A4Z2DB233.164e-4644.44FMRFamide-activated amiloride-sensitive sodium cha... [more]
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BLAST of FMRFamide-activated amiloride-sensitive sodium channel vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of FMRFamide-activated amiloride-sensitive sodium channel vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of FMRFamide-activated amiloride-sensitive sodium channel vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of FMRFamide-activated amiloride-sensitive sodium channel vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of FMRFamide-activated amiloride-sensitive sodium channel vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of FMRFamide-activated amiloride-sensitive sodium channel vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000069611.11.458e-15395.54SMESG000069611.1[more]
SMESG000028687.13.984e-4644.44SMESG000028687.1[more]
SMESG000078360.11.286e-4544.51SMESG000078360.1[more]
SMESG000044381.11.165e-4344.00SMESG000044381.1[more]
SMESG000037525.12.000e-4250.30SMESG000037525.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30023439 ID=SMED30023439|Name=FMRFamide-activated amiloride-sensitive sodium channel|organism=Schmidtea mediterranea sexual|type=transcript|length=891bp
GAGAAAATAAAGCTCAATTTTGATTGGATGATTTTGGAAAATAGAGTTTC
ATTTAAAAACTAATATTTTAAAAATTCATTTTCATAATGCACGGCAATAA
TCTGCTCCAAGAACCGCAATTACTGAAAAATGTCTGCTGGTTTTGCAAGA
GCTCAGTAAAAACGACTCTGGAGGATTTTCATTCAAATCAAATCCAGGAA
GAGGCGATTTTAAATTCTCCGACGTTCAAGAAATTATTAAAAAATGAACT
GCTTCATTTCTGTAAGAACACAACAATCCGTGGAGTTCCGAGAATTGTCA
ACACTACCAACAAATCGAGTAAAATTCTATGGATAAGTTTTTTTATTTTT
CTCTGTGCCGGTTTGACAATCTGTCTGACATTTCTCATCCGACAATTTAT
TGAATATAGTGTCATTCATCCACCAAGAGTTCTTCAAAACAGCCCGTCTC
CGTTTCCGTCATTTACTGTCTGTAATCATCGGCCATTGAGTTACAATATC
AGCAGTTTTTTCAATCAAAGAAACATGAGAAATCCTCGAGAATTTGCAAT
AGCGGCCAACTACGTGTCGAAAAAGTATCTATTTGATCGTGGAGATAAAG
GTCATTATAGTTTGATTACTTCAAGCTTTTCTTTGAGCGGTTATTTGGAA
AGCATTCCATTGGAATATCATAATATGCTTGGACACGACTACTACGATTT
TGTTATTGACTGTTTACTAATAAATACTGTCAGAAGACAAGGAAGTAAAC
AGTCAATTCTGAACCACAATTCATTGACAGTTTACTGTCCATACCAAATC
ATTTTTATGAGGACAACTATATGTTGGGCTGCATGACGAATTTGAAAAAG
GACAAAAACATCTAATTTTTTTCTCTAGGGCTTTTAGTATA
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protein sequence of SMED30023439-orf-1

>SMED30023439-orf-1 ID=SMED30023439-orf-1|Name=SMED30023439-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=250bp
MHGNNLLQEPQLLKNVCWFCKSSVKTTLEDFHSNQIQEEAILNSPTFKKL
LKNELLHFCKNTTIRGVPRIVNTTNKSSKILWISFFIFLCAGLTICLTFL
IRQFIEYSVIHPPRVLQNSPSPFPSFTVCNHRPLSYNISSFFNQRNMRNP
REFAIAANYVSKKYLFDRGDKGHYSLITSSFSLSGYLESIPLEYHNMLGH
DYYDFVIDCLLINTVRRQGSKQSILNHNSLTVYCPYQIIFMRTTICWAA*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000101muscle cell
PLANA:0000140anterior region of the whole animal
Vocabulary: cellular component
TermDefinition
GO:0016020membrane
GO:0016021integral component of membrane
Vocabulary: biological process
TermDefinition
GO:0006814sodium ion transport
GO:0006810transport
GO:0006811ion transport
GO:0035725sodium ion transmembrane transport
Vocabulary: molecular function
TermDefinition
GO:0005272sodium channel activity
Vocabulary: INTERPRO
TermDefinition
IPR001873ENaC
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001873Epithelial sodium channelPFAMPF00858ASCcoord: 58..214
e-value: 8.3E-18
score: 64.6
NoneNo IPR availableGENE3DG3DSA:1.10.287.770coord: 79..108
e-value: 2.4E-8
score: 35.4
NoneNo IPR availableGENE3DG3DSA:2.60.470.10coord: 109..210
e-value: 2.4E-8
score: 35.4
NoneNo IPR availableGENE3DG3DSA:1.10.3590.10coord: 132..200
e-value: 2.4E-8
score: 35.4
NoneNo IPR availableTMHMMTMhelixcoord: 80..102