SMED30023433

Overview
NameSMED30023433
Smed IDSMED30023433
Length (bp)2216
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30023433 (SMED30023433) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30023433 (SMED30023433) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30023433 (SMED30023433) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30023433 (SMED30023433) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30023433

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 4

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
neoblastSMED30023433SMESG000067632.1 dd_Smed_v4_6224_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30023433SMESG000067632.1 dd_Smed_v4_6224_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30023433SMESG000067632.1 dd_Smed_v4_6224_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
ciliated epithelial cellSMED30023433SMESG000067632.1 dd_Smed_v4_6224_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of SMED30023433 vs. Ensembl Human
Match: NRXN1 (neurexin 1 [Source:HGNC Symbol;Acc:HGNC:8008])

HSP 1 Score: 54.299 bits (129), Expect = 8.501e-7
Identity = 32/100 (32.00%), Postives = 47/100 (47.00%), Query Frame = 3 
Query:  690 FWIGNVPSENEENLYQNIVHWKSNRLNLWGCVKLFQIKNKILKLASSGNFFGEMIQPFCK---PICKANSCFNNGKCLELWRGRFICDCGFSQFVGERCN 980
             +IG V  E  ++L + +VH K       GC+    +  ++  L S   F    I+  C+     C+ +SC N G CL+ W G F CDC  + F G  CN
Sbjct:  744 LYIGGVAKETYKSLPK-LVHAKEG---FQGCLASVDLNGRLPDLISDALFCNGQIERGCEGPSTTCQEDSCSNQGVCLQQWDG-FSCDCSMTSFSGPLCN 838
[Close]
BLAST of SMED30023433 vs. Ensembl Human
Match: NRXN1 (neurexin 1 [Source:HGNC Symbol;Acc:HGNC:8008])

HSP 1 Score: 53.9138 bits (128), Expect = 1.016e-6
Identity = 32/100 (32.00%), Postives = 47/100 (47.00%), Query Frame = 3 
Query:  690 FWIGNVPSENEENLYQNIVHWKSNRLNLWGCVKLFQIKNKILKLASSGNFFGEMIQPFCK---PICKANSCFNNGKCLELWRGRFICDCGFSQFVGERCN 980
             +IG V  E  ++L + +VH K       GC+    +  ++  L S   F    I+  C+     C+ +SC N G CL+ W G F CDC  + F G  CN
Sbjct:  756 LYIGGVAKETYKSLPK-LVHAKEG---FQGCLASVDLNGRLPDLISDALFCNGQIERGCEGPSTTCQEDSCSNQGVCLQQWDG-FSCDCSMTSFSGPLCN 850
[Close]
BLAST of SMED30023433 vs. Ensembl Human
Match: NRXN1 (neurexin 1 [Source:HGNC Symbol;Acc:HGNC:8008])

HSP 1 Score: 53.9138 bits (128), Expect = 1.045e-6
Identity = 32/100 (32.00%), Postives = 47/100 (47.00%), Query Frame = 3 
Query:  690 FWIGNVPSENEENLYQNIVHWKSNRLNLWGCVKLFQIKNKILKLASSGNFFGEMIQPFCK---PICKANSCFNNGKCLELWRGRFICDCGFSQFVGERCN 980
             +IG V  E  ++L + +VH K       GC+    +  ++  L S   F    I+  C+     C+ +SC N G CL+ W G F CDC  + F G  CN
Sbjct:  741 LYIGGVAKETYKSLPK-LVHAKEG---FQGCLASVDLNGRLPDLISDALFCNGQIERGCEGPSTTCQEDSCSNQGVCLQQWDG-FSCDCSMTSFSGPLCN 835
[Close]
BLAST of SMED30023433 vs. Ensembl Human
Match: NRXN1 (neurexin 1 [Source:HGNC Symbol;Acc:HGNC:8008])

HSP 1 Score: 53.5286 bits (127), Expect = 1.542e-6
Identity = 32/100 (32.00%), Postives = 47/100 (47.00%), Query Frame = 3 
Query:  690 FWIGNVPSENEENLYQNIVHWKSNRLNLWGCVKLFQIKNKILKLASSGNFFGEMIQPFCK---PICKANSCFNNGKCLELWRGRFICDCGFSQFVGERCN 980
             +IG V  E  ++L + +VH K       GC+    +  ++  L S   F    I+  C+     C+ +SC N G CL+ W G F CDC  + F G  CN
Sbjct: 1026 LYIGGVAKETYKSLPK-LVHAKEG---FQGCLASVDLNGRLPDLISDALFCNGQIERGCEGPSTTCQEDSCSNQGVCLQQWDG-FSCDCSMTSFSGPLCN 1120
[Close]
BLAST of SMED30023433 vs. Ensembl Human
Match: NRXN1 (neurexin 1 [Source:HGNC Symbol;Acc:HGNC:8008])

HSP 1 Score: 52.373 bits (124), Expect = 3.406e-6
Identity = 32/100 (32.00%), Postives = 46/100 (46.00%), Query Frame = 3 
Query:  690 FWIGNVPSENEENLYQNIVHWKSNRLNLWGCVKLFQIKNKILKLASSGNFFGEMIQPFCK---PICKANSCFNNGKCLELWRGRFICDCGFSQFVGERCN 980
             +IG V  E  ++L   +VH K       GC+    +  ++  L S   F    I+  C+     C+ +SC N G CL+ W G F CDC  + F G  CN
Sbjct: 1014 LYIGGVAKETYKSL-PKLVHAKEG---FQGCLASVDLNGRLPDLISDALFCNGQIERGCEGPSTTCQEDSCSNQGVCLQQWDG-FSCDCSMTSFSGPLCN 1108
[Close]
BLAST of SMED30023433 vs. Ensembl Zebrafish
Match: nrxn3b (neurexin 3b [Source:NCBI gene;Acc:570698])

HSP 1 Score: 52.373 bits (124), Expect = 2.073e-6
Identity = 25/88 (28.41%), Postives = 42/88 (47.73%), Query Frame = 3 
Query:  726 NLYQNIVHWKSNRLNLWGCVKLFQIKNKILKLASSGNFFGEMIQPFCK---PICKANSCFNNGKCLELWRGRFICDCGFSQFVGERCN 980
            N+YQN+     +R    GC+    +  ++  L +   F    I+  C+     C+ +SC N G C++ W   F CDC  + + G +CN
Sbjct: 1003 NMYQNLPKLVVSREGFQGCLASMDLNGRLPDLINDALFRSGQIERGCEGPSTTCQEDSCANMGVCIQQWE-NFTCDCSMTSYSGTQCN 1089
[Close]
BLAST of SMED30023433 vs. Ensembl Zebrafish
Match: nrxn3b (neurexin 3b [Source:NCBI gene;Acc:570698])

HSP 1 Score: 52.373 bits (124), Expect = 2.243e-6
Identity = 25/88 (28.41%), Postives = 42/88 (47.73%), Query Frame = 3 
Query:  726 NLYQNIVHWKSNRLNLWGCVKLFQIKNKILKLASSGNFFGEMIQPFCK---PICKANSCFNNGKCLELWRGRFICDCGFSQFVGERCN 980
            N+YQN+     +R    GC+    +  ++  L +   F    I+  C+     C+ +SC N G C++ W   F CDC  + + G +CN
Sbjct: 1003 NMYQNLPKLVVSREGFQGCLASMDLNGRLPDLINDALFRSGQIERGCEGPSTTCQEDSCANMGVCIQQWEN-FTCDCSMTSYSGTQCN 1089
[Close]
BLAST of SMED30023433 vs. Ensembl Mouse
Match: Nrxn1 (neurexin I [Source:MGI Symbol;Acc:MGI:1096391])

HSP 1 Score: 50.8322 bits (120), Expect = 7.465e-7
Identity = 32/100 (32.00%), Postives = 47/100 (47.00%), Query Frame = 3 
Query:  690 FWIGNVPSENEENLYQNIVHWKSNRLNLWGCVKLFQIKNKILKLASSGNFFGEMIQPFCK---PICKANSCFNNGKCLELWRGRFICDCGFSQFVGERCN 980
             +IG V  E  ++L + +VH K       GC+    +  ++  L S   F    I+  C+     C+ +SC N G CL+ W G F CDC  + F G  CN
Sbjct:   11 LYIGGVAKETYKSLPK-LVHAKEG---FQGCLASVDLNGRLPDLISDALFCNGQIERGCEGPSTTCQEDSCSNQGVCLQQWDG-FSCDCSMTSFSGPLCN 105
[Close]
BLAST of SMED30023433 vs. Ensembl Mouse
Match: Nrxn1 (neurexin I [Source:MGI Symbol;Acc:MGI:1096391])

HSP 1 Score: 53.9138 bits (128), Expect = 9.479e-7
Identity = 32/100 (32.00%), Postives = 47/100 (47.00%), Query Frame = 3 
Query:  690 FWIGNVPSENEENLYQNIVHWKSNRLNLWGCVKLFQIKNKILKLASSGNFFGEMIQPFCK---PICKANSCFNNGKCLELWRGRFICDCGFSQFVGERCN 980
             +IG V  E  ++L + +VH K       GC+    +  ++  L S   F    I+  C+     C+ +SC N G CL+ W G F CDC  + F G  CN
Sbjct: 1026 LYIGGVAKETYKSLPK-LVHAKEG---FQGCLASVDLNGRLPDLISDALFCNGQIERGCEGPSTTCQEDSCSNQGVCLQQWDG-FSCDCSMTSFSGPLCN 1120
[Close]
BLAST of SMED30023433 vs. Ensembl Mouse
Match: Nrxn1 (neurexin I [Source:MGI Symbol;Acc:MGI:1096391])

HSP 1 Score: 52.373 bits (124), Expect = 2.338e-6
Identity = 32/100 (32.00%), Postives = 46/100 (46.00%), Query Frame = 3 
Query:  690 FWIGNVPSENEENLYQNIVHWKSNRLNLWGCVKLFQIKNKILKLASSGNFFGEMIQPFCK---PICKANSCFNNGKCLELWRGRFICDCGFSQFVGERCN 980
             +IG V  E  ++L   +VH K       GC+    +  ++  L S   F    I+  C+     C+ +SC N G CL+ W G F CDC  + F G  CN
Sbjct: 1014 LYIGGVAKETYKSL-PKLVHAKEG---FQGCLASVDLNGRLPDLISDALFCNGQIERGCEGPSTTCQEDSCSNQGVCLQQWDG-FSCDCSMTSFSGPLCN 1108
[Close]
BLAST of SMED30023433 vs. Ensembl Mouse
Match: Nrxn1 (neurexin I [Source:MGI Symbol;Acc:MGI:1096391])

HSP 1 Score: 52.373 bits (124), Expect = 2.339e-6
Identity = 32/100 (32.00%), Postives = 46/100 (46.00%), Query Frame = 3 
Query:  690 FWIGNVPSENEENLYQNIVHWKSNRLNLWGCVKLFQIKNKILKLASSGNFFGEMIQPFCK---PICKANSCFNNGKCLELWRGRFICDCGFSQFVGERCN 980
             +IG V  E  ++L   +VH K       GC+    +  ++  L S   F    I+  C+     C+ +SC N G CL+ W G F CDC  + F G  CN
Sbjct: 1018 LYIGGVAKETYKSL-PKLVHAKEG---FQGCLASVDLNGRLPDLISDALFCNGQIERGCEGPSTTCQEDSCSNQGVCLQQWDG-FSCDCSMTSFSGPLCN 1112
[Close]
BLAST of SMED30023433 vs. Ensembl Mouse
Match: Nrxn1 (neurexin I [Source:MGI Symbol;Acc:MGI:1096391])

HSP 1 Score: 52.373 bits (124), Expect = 2.339e-6
Identity = 32/100 (32.00%), Postives = 46/100 (46.00%), Query Frame = 3 
Query:  690 FWIGNVPSENEENLYQNIVHWKSNRLNLWGCVKLFQIKNKILKLASSGNFFGEMIQPFCK---PICKANSCFNNGKCLELWRGRFICDCGFSQFVGERCN 980
             +IG V  E  ++L   +VH K       GC+    +  ++  L S   F    I+  C+     C+ +SC N G CL+ W G F CDC  + F G  CN
Sbjct: 1018 LYIGGVAKETYKSL-PKLVHAKEG---FQGCLASVDLNGRLPDLISDALFCNGQIERGCEGPSTTCQEDSCSNQGVCLQQWDG-FSCDCSMTSFSGPLCN 1112
[Close]
BLAST of SMED30023433 vs. UniProt/SwissProt
Match: sp|Q9ULB1|NRX1A_HUMAN (Neurexin-1 OS=Homo sapiens OX=9606 GN=NRXN1 PE=1 SV=1)

HSP 1 Score: 53.5286 bits (127), Expect = 7.406e-6
Identity = 32/100 (32.00%), Postives = 47/100 (47.00%), Query Frame = 3 
Query:  690 FWIGNVPSENEENLYQNIVHWKSNRLNLWGCVKLFQIKNKILKLASSGNFFGEMIQPFCK---PICKANSCFNNGKCLELWRGRFICDCGFSQFVGERCN 980
             +IG V  E  ++L + +VH K       GC+    +  ++  L S   F    I+  C+     C+ +SC N G CL+ W G F CDC  + F G  CN
Sbjct: 1026 LYIGGVAKETYKSLPK-LVHAKEG---FQGCLASVDLNGRLPDLISDALFCNGQIERGCEGPSTTCQEDSCSNQGVCLQQWDG-FSCDCSMTSFSGPLCN 1120
[Close]
BLAST of SMED30023433 vs. UniProt/SwissProt
Match: sp|Q9DDD0|NRX1A_CHICK (Neurexin-1 (Fragment) OS=Gallus gallus OX=9031 GN=NRXN1 PE=2 SV=1)

HSP 1 Score: 53.1434 bits (126), Expect = 9.428e-6
Identity = 32/100 (32.00%), Postives = 47/100 (47.00%), Query Frame = 3 
Query:  690 FWIGNVPSENEENLYQNIVHWKSNRLNLWGCVKLFQIKNKILKLASSGNFFGEMIQPFCK---PICKANSCFNNGKCLELWRGRFICDCGFSQFVGERCN 980
             +IG V  E  ++L + +VH K       GC+    +  ++  L S   F    I+  C+     C+ +SC N G CL+ W G F CDC  + F G  CN
Sbjct:  882 LYIGGVAKEMYKSLPK-LVHAKEG---FQGCLASVDLNGRLPDLISDALFCNGQIERGCEGPSTTCQEDSCANQGVCLQQWDG-FSCDCSMTSFSGPLCN 976
[Close]
BLAST of SMED30023433 vs. TrEMBL
Match: A0A158QKH5 (Uncharacterized protein OS=Haemonchus placei OX=6290 GN=HPLM_LOCUS4581 PE=4 SV=1)

HSP 1 Score: 66.2402 bits (160), Expect = 2.308e-7
Identity = 46/176 (26.14%), Postives = 76/176 (43.18%), Query Frame = 3 
Query:  657 PVPYFNETAVHFWIGNVPSENEENLYQNIVHWKSNRLNLWGCVKLFQIKNKILKLA----SSGNFFGEMIQPFCKPICKANSCFNNGKCLELWR---GRFICDCGFSQFVGERCNLDHSVKF--NRSFSFQHSIYFK---------LQKVQFAFQPVIDDQHRGHKMLSLKLFKNQ 1130
            P P+      + +IG +  EN   L + I  ++    NL GC++  ++  +++ L      SG     +++  C   C    C N G C   WR   G+ +CDC  + + G  C +D  + F  N  F+F    +            QK+QFAF P    Q   H+ L+  LF ++
Sbjct:  806 PKPFL-----YTFIGGIERENGSMLLRPI--YQCAVPNLLGCMRGVRVGGEVVDLRHTIHDSGPNDQNLVRSGCDMGCATLDCRNGGHCSVAWRGVGGKVVCDCSRTSYAGPHCTIDEGLTFDSNAHFTFDIGRFLSRYILTPQKLTQKLQFAFAPTSPSQT--HQTLATVLFNDK 972
[Close]
BLAST of SMED30023433 vs. TrEMBL
Match: A0A0B7ATQ3 (Uncharacterized protein OS=Arion vulgaris OX=1028688 GN=ORF139905 PE=4 SV=1)

HSP 1 Score: 65.0846 bits (157), Expect = 7.247e-7
Identity = 45/178 (25.28%), Postives = 74/178 (41.57%), Query Frame = 3 
Query:  654 FPVPYFNETAVHFWIGNVPSENEENLYQNIVHWKSNRLNLWGCVKLFQIKNKILKLASSGNFFG--EMIQPFCKPICKANSCFNNGKCLELWRGR-FICDCGFSQFVGERCNLDHSVKFNRSFSFQHSI-------YFKLQKVQFAFQPVIDDQHRGHKMLSL--KLFKNQYIYIYLD 1151
             P PY     +H +IG             I    S+  ++ GC++  +  + +  L  +    G   ++   C   C  NSC NNG CLE W  + F CDC  + F G RC ++ SVK       QH+        Y   +++ F F+  +   +  + +L+       N Y+ + LD
Sbjct: 1251 MPAPY-----IHAYIGG------------IKDMASSVNSMVGCIRGVRAGDHVFHLQDAAVALGNDTLVDQECNKGCVENSCHNNGICLEKWGNKQFQCDCAGNGFSGNRCEIEPSVKLTGKTVIQHTFQLSIMDQYSLTERLSFRFKTELKLDNSRNSILAFVTSTISNDYVLVKLD 1411
[Close]
BLAST of SMED30023433 vs. TrEMBL
Match: A0A2C9LN94 (Uncharacterized protein OS=Biomphalaria glabrata OX=6526 GN=106065004 PE=4 SV=1)

HSP 1 Score: 64.3142 bits (155), Expect = 1.270e-6
Identity = 34/111 (30.63%), Postives = 53/111 (47.75%), Query Frame = 3 
Query:  684 VHFWIGNVPSENEENLYQNIVHWKSNRLNLWGCVKLFQIKNKI--LKLASSGNFFGEMIQPFCKPICKANSCFNNGKCLELWRGR-FICDCGFSQFVGERCNLDHSVKFNR 1007
            +H ++G VP +N            S    L GC++  +  N +  L+ A+       ++ P C+  C  N+C N G C+E WR R F CDC  + F G  C  + S+ FN+
Sbjct: 1142 IHAYVGGVPGKN------------SYASRLTGCIRGVRAGNYVFHLRKAAEAVNNDTLVAPMCESGCGENTCLNKGFCVEKWRNRTFECDCSDNGFTGFHCEKEPSISFNK 1240
[Close]
BLAST of SMED30023433 vs. TrEMBL
Match: W2TTR5 (EGF-like domain protein OS=Necator americanus OX=51031 GN=NECAME_16887 PE=4 SV=1)

HSP 1 Score: 58.9214 bits (141), Expect = 2.503e-6
Identity = 41/149 (27.52%), Postives = 66/149 (44.30%), Query Frame = 3 
Query:  768 NLWGCVKLFQIKNKILKLASSGNFFG----------EMIQPFCKPICKANSCFNNGKCLELW--RGRFICDCGFSQFVGERCNLDHSVKF--NRSFSFQHSIYF---------KLQKVQFAFQPVIDDQHRGHKMLSLKLFKNQYIYIY 1145
            NL GCV+  +I  +I+ L  +G+ F           E+I+  C+  C +  C N+G C   W   G   CDC  + + G  C +D+ +    N  F+F    +          + Q +QFAF P     H  H++L+   FK++    Y
Sbjct:   13 NLLGCVRGLRIGGEIVDLRQTGHGFRPNDPSWLYDYELIRIGCELGCSSLDCKNSGHCSVGWLGNGDVTCDCSRTSYAGSDCTVDNGLILAANSYFTFDMDRFLSRYILSPMKRTQTLQFAFAPSSPSTH--HQILATVQFKDERSVTY 159
[Close]
BLAST of SMED30023433 vs. TrEMBL
Match: A0A433TUD4 (Uncharacterized protein OS=Elysia chlorotica OX=188477 GN=EGW08_007075 PE=4 SV=1)

HSP 1 Score: 62.7734 bits (151), Expect = 3.165e-6
Identity = 31/98 (31.63%), Postives = 54/98 (55.10%), Query Frame = 3 
Query:  771 LWGCVKLFQIKNKILKLASSGNFF--GEMIQPFCKPICKANSCFNNGKCLELWR-GRFICDCGFSQFVGERCNLDHSVKFNRSFSFQHSIYFKLQKVQ 1055
            L GC++  ++ N +  L  S +    G ++ P C+  C+  +C NNG C+E W+ G+F C+C  S F G+RC ++ SV    +    H+  F+L + +
Sbjct: 1360 LSGCLRGLRVGNFVFPLQDSASSLKNGTLVTPVCEKGCQKMACHNNGFCVEKWKNGQFQCNCDKSGFSGQRCEIEPSVLVKGNTVVHHT--FQLPRAE 1455
[Close]
BLAST of SMED30023433 vs. Ensembl Cavefish
Match: ENSAMXT00000021549.2 (pep primary_assembly:Astyanax_mexicanus-2.0:14:7994995:8101472:1 gene:ENSAMXG00000020839.2 transcript:ENSAMXT00000021549.2 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 55.0694 bits (131), Expect = 2.410e-7
Identity = 70/300 (23.33%), Postives = 114/300 (38.00%), Query Frame = 3 
Query:  411 FVQEMLEISYYNFMISEANRGYFRIKDLCIPINDNKLI-LNFVSKNNEIIISCNGNSRTLDYTQPPEIVSENELRTFNSYMNFPVPYFNETAVHFWIGNVPSENEENLYQNIVHWKSNRLNLWGCVKLFQIKNK---ILKLASSGNFFGEMIQPFCKP----ICKANSCFNNGKCLELWRGRFICDCGFSQFVGERCNLDHSVKFNRSFSFQHSIYFKLQKVQFAFQPVIDDQHRGHKMLSLKLFKNQYIYIYLDIEYFFHVRFEQNTDWIFDEKFRMKLYNSRMYLVFIDVNIDEIRIQ 1286
            F  E+LE   Y  M  +   G  ++K     +ND +   ++F  +  +  +S NG S      +  E++  +                       ++G +P E+E  L    V     RL   GCV+   +  +   + +LA   +  G  +  FC       C A  C + G C E W  R ICDC  + F+G  C  + +V      S+  S++ K+        P    QH   + +SL+ F +Q  Y  L             T     +  R++L   R+ L    VN+D I I 
Sbjct:  289 FAMELLEGFLYLLM--DMGSGSIKMKASTKKVNDGEWCHVDFQREGRKGSVSVNGRSMPFSTNEGSEVLDLDG--------------------DMYLGGLPEESEGLLLPPEVWTAPLRLGFVGCVRDLFVDGRSRDLRRLAELQSAVG--VSSFCTRETHRRCSAEPCAHGGHCREGWN-RHICDCSGTGFLGPNCETESTV-----LSYDGSMFLKV------LMPRA--QHTEAEDVSLR-FMSQRAYGLL----------MATTSSESADTLRLELDGGRVKLT---VNLDCIGID 536
[Close]
BLAST of SMED30023433 vs. Ensembl Cavefish
Match: ENSAMXT00000043111.1 (pep primary_assembly:Astyanax_mexicanus-2.0:14:7993674:8103549:1 gene:ENSAMXG00000020839.2 transcript:ENSAMXT00000043111.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 54.6842 bits (130), Expect = 3.644e-7
Identity = 51/219 (23.29%), Postives = 86/219 (39.27%), Query Frame = 3 
Query:  411 FVQEMLEISYYNFMISEANRGYFRIKDLCIPINDNKLI-LNFVSKNNEIIISCNGNSRTLDYTQPPEIVSENELRTFNSYMNFPVPYFNETAVHFWIGNVPSENEENLYQNIVHWKSNRLNLWGCVKLFQIKNK---ILKLASSGNFFGEMIQPFCKP----ICKANSCFNNGKCLELWRGRFICDCGFSQFVGERCNLDHSVKFNRSFSFQHSIYFKL 1043
            F  E+LE   Y  M  +   G  ++K     +ND +   ++F  +  +  +S NG S      +  E++  +                       ++G +P E+E  L    V     RL   GCV+   +  +   + +LA   +  G  +  FC       C A  C + G C E W  R ICDC  + F+G  C  + +V      S+  S++ K+
Sbjct:  260 FAMELLEGFLYLLM--DMGSGSIKMKASTKKVNDGEWCHVDFQREGRKGSVSVNGRSMPFSTNEGSEVLDLDG--------------------DMYLGGLPEESEGLLLPPEVWTAPLRLGFVGCVRDLFVDGRSRDLRRLAELQSAVG--VSSFCTRETHRRCSAEPCAHGGHCREGWN-RHICDCSGTGFLGPNCETESTV-----LSYDGSMFLKV 448
[Close]
BLAST of SMED30023433 vs. Ensembl Cavefish
Match: ENSAMXT00000050294.1 (pep primary_assembly:Astyanax_mexicanus-2.0:14:7994312:8101472:1 gene:ENSAMXG00000020839.2 transcript:ENSAMXT00000050294.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 54.299 bits (129), Expect = 4.598e-7
Identity = 51/219 (23.29%), Postives = 86/219 (39.27%), Query Frame = 3 
Query:  411 FVQEMLEISYYNFMISEANRGYFRIKDLCIPINDNKLI-LNFVSKNNEIIISCNGNSRTLDYTQPPEIVSENELRTFNSYMNFPVPYFNETAVHFWIGNVPSENEENLYQNIVHWKSNRLNLWGCVKLFQIKNK---ILKLASSGNFFGEMIQPFCKP----ICKANSCFNNGKCLELWRGRFICDCGFSQFVGERCNLDHSVKFNRSFSFQHSIYFKL 1043
            F  E+LE   Y  M  +   G  ++K     +ND +   ++F  +  +  +S NG S      +  E++                    +     ++G +P E+E  L    V     RL   GCV+   +  +   + +LA   +  G  +  FC       C A  C + G C E W  R ICDC  + F+G  C  + +V      S+  S++ K+
Sbjct:  267 FAMELLEGFLYLLM--DMGSGSIKMKASTKKVNDGEWCHVDFQREGRKGSVSVNGRSMPFSTNEGSEVL--------------------DLDGDMYLGGLPEESEGLLLPPEVWTAPLRLGFVGCVRDLFVDGRSRDLRRLAELQSAVG--VSSFCTRETHRRCSAEPCAHGGHCREGWN-RHICDCSGTGFLGPNCETESTV-----LSYDGSMFLKV 455
[Close]
BLAST of SMED30023433 vs. Ensembl Cavefish
Match: nrxn3b (neurexin 3b [Source:NCBI gene;Acc:570698])

HSP 1 Score: 50.8322 bits (120), Expect = 6.330e-6
Identity = 25/88 (28.41%), Postives = 41/88 (46.59%), Query Frame = 3 
Query:  726 NLYQNIVHWKSNRLNLWGCVKLFQIKNKILKLASSGNFFGEMIQPFCK---PICKANSCFNNGKCLELWRGRFICDCGFSQFVGERCN 980
            N+YQN+     +R    GC+    +  ++  L +   F    I+  C+     C+ +SC N G C++ W   F CDC  + + G  CN
Sbjct:  976 NMYQNLPKLVVSREGFQGCLASVDLNGRLPDLINDALFSSGQIEHGCEGPSTTCQEDSCANYGVCIQQWE-NFTCDCSMTSYSGVTCN 1062
[Close]
BLAST of SMED30023433 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000002142.1 (pep scaffold:Pmarinus_7.0:GL476386:209124:506099:1 gene:ENSPMAG00000001935.1 transcript:ENSPMAT00000002142.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 55.0694 bits (131), Expect = 6.196e-8
Identity = 54/209 (25.84%), Postives = 95/209 (45.45%), Query Frame = 3 
Query:  687 HFWIGNVPSENEENLYQNIVHWKSNRLNLW--GCVKLFQIKNK---ILKLASSGNFFGEMIQPFCKPI----CKANSCFNNGKCLELWRGRFICDCGFSQFVGERCNLDHSVKFNRSFSFQHSIYFKLQKVQFAFQPVIDDQHRGHKMLSLKLFKNQYIYIYLDIEYFFHVRFEQNTDWIFDEKFRMKLYNSRMYLVFIDVNIDEIRIQ 1286
              ++G +P E++ +L      W S  LN+   GCV+   I  +   + +LA   N  G  ++PFC       C +N C N+G C E W  R++CDC  + + G  C  + S+      S+  S++ K+        PV+   H   + ++L+ F +Q  Y  L             T     +  R++L ++R+ L    +N+D +RI+
Sbjct:  342 EVFLGGLP-EDKADLLLPTELW-SALLNIGYVGCVRDLFIDGQSKDVRQLAEQQNAAG--VKPFCAKDSPKQCSSNPCRNHGVCREGWN-RYVCDCTATGYTGRSCEREASI-----LSYDGSMFMKV------VMPVV--IHTEAEDVTLR-FVSQRAYGLL----------LATTSRDSADTLRLELDSARVKLT---INLDCVRIK 518
[Close]
BLAST of SMED30023433 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000004299.1 (pep scaffold:Pmarinus_7.0:GL477235:143017:163597:1 gene:ENSPMAG00000003933.1 transcript:ENSPMAT00000004299.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 48.521 bits (114), Expect = 3.208e-6
Identity = 25/100 (25.00%), Postives = 46/100 (46.00%), Query Frame = 3 
Query:  690 FWIGNVPSENEENLYQNIVHWKSNRLNLWGCVKLFQIKNKILKLASSGNFFGEMIQPFCK---PICKANSCFNNGKCLELWRGRFICDCGFSQFVGERCN 980
             ++G +P E+   L++ +V    +R    GC+    +  ++  L          I+  C+    +C+ +SC N G C++ W   F CDC  + + G  CN
Sbjct:  239 LFVGGLPREHYSGLHKLVV----SRDGFQGCLASVDLNGRLPDLLDDALRRSGQIERGCEGPSTVCQDDSCSNQGLCVQQWE-EFTCDCTMTSYTGAFCN 333
[Close]
BLAST of SMED30023433 vs. Ensembl Medaka
Match: nrxn3b (neurexin-3b [Source:NCBI gene;Acc:101156134])

HSP 1 Score: 53.5286 bits (127), Expect = 8.718e-7
Identity = 26/88 (29.55%), Postives = 41/88 (46.59%), Query Frame = 3 
Query:  726 NLYQNIVHWKSNRLNLWGCVKLFQIKNKILKLASSGNFFGEMIQPFCK---PICKANSCFNNGKCLELWRGRFICDCGFSQFVGERCN 980
            N+YQN+     +R    GC+    +  ++  L S   F    I+  C+     C+ +SC N G C++ W   F CDC  + + G  CN
Sbjct: 1007 NMYQNLPKLVVSREGFQGCLATVDLNGRLPDLISDALFRSGQIERGCEGPSTTCQEDSCANMGVCIQQWE-NFTCDCSMTSYTGTYCN 1093
[Close]
BLAST of SMED30023433 vs. Ensembl Medaka
Match: nrxn3b (neurexin-3b [Source:NCBI gene;Acc:101156134])

HSP 1 Score: 53.5286 bits (127), Expect = 8.781e-7
Identity = 26/88 (29.55%), Postives = 41/88 (46.59%), Query Frame = 3 
Query:  726 NLYQNIVHWKSNRLNLWGCVKLFQIKNKILKLASSGNFFGEMIQPFCK---PICKANSCFNNGKCLELWRGRFICDCGFSQFVGERCN 980
            N+YQN+     +R    GC+    +  ++  L S   F    I+  C+     C+ +SC N G C++ W   F CDC  + + G  CN
Sbjct:  976 NMYQNLPKLVVSREGFQGCLATVDLNGRLPDLISDALFRSGQIERGCEGPSTTCQEDSCANMGVCIQQWE-NFTCDCSMTSYTGTYCN 1062
[Close]
BLAST of SMED30023433 vs. Ensembl Medaka
Match: nrxn3b (neurexin-3b [Source:NCBI gene;Acc:101156134])

HSP 1 Score: 53.5286 bits (127), Expect = 8.788e-7
Identity = 26/88 (29.55%), Postives = 41/88 (46.59%), Query Frame = 3 
Query:  726 NLYQNIVHWKSNRLNLWGCVKLFQIKNKILKLASSGNFFGEMIQPFCK---PICKANSCFNNGKCLELWRGRFICDCGFSQFVGERCN 980
            N+YQN+     +R    GC+    +  ++  L S   F    I+  C+     C+ +SC N G C++ W   F CDC  + + G  CN
Sbjct:  986 NMYQNLPKLVVSREGFQGCLATVDLNGRLPDLISDALFRSGQIERGCEGPSTTCQEDSCANMGVCIQQWE-NFTCDCSMTSYTGTYCN 1072
[Close]
BLAST of SMED30023433 vs. Planmine SMEST
Match: SMESG000067632.1 (SMESG000067632.1)

HSP 1 Score: 1147.11 bits (2966), Expect = 0.000e+0
Identity = 607/614 (98.86%), Postives = 610/614 (99.35%), Query Frame = 3 
Query:  168 MAFIYQFLLGWMLSLISAYDQXXXXXXXXXXXXXXYWHGKMAILRSKSFIEEGILLKVQLNQFQGLLFYAIDSNYRTIWVEFVQEMLEISYYNFMISEANRGYFRIKDLCIPINDNKLILNFVSKNNEIIISCNGNSRTLDYTQPPEIVSENELRTFNSYMNFPVPYFNETAVHFWIGNVPSENEENLYQNIVHWKSNRLNLWGCVKLFQIKNKILKLASSGNFFGEMIQPFCKPICKANSCFNNGKCLELWRGRFICDCGFSQFVGERCNLDHSVKFNRSFSFQHSIYFKLQKVQFAFQPVIDDQHRGHKMLSLKLFKNQYIYIYLDIEYFFHVRFEQNTDWIFDEKFRMKLYNSRMYLVFIDVNIDEIRIQLFDALDKSLFERKIIQHQESNLVITDILVGDSHSHGMGWMGKYEGCLTNLMLKLDSRKFIWNEIRNFDKFPNCKEAARTTLKPNQTXXXXXXXXXXXXXAVLLSKTLNGTENIPRNNDYLIYXXXXXXXXXXXXXXXXXXXXXKFKLTNQKSFSESSDKIRLTDNFNKSLNHSELPEQQQIITNSLCNSPTNSNHIYPSPIYMTFKPAYKAKSMTSSITNRSVMSLPNPTSPTLATQEESE 2009
            MAFIYQFLLGWMLSLISAYDQSISFSPISKSFPNSYWHGKMAILRSKSFIEEGILLKVQLNQFQGLLFYAIDSNYRTIWVEFVQEMLEISYYNFMISEANRGYFRIKDLCIPINDNKLILNFVSKNNEIIISCNGNSRTLDYTQPPEIVSENELRTFNSYMNFPVPYFNET VHFWIGNVPSENEENLYQNIVHWKSNRLNLWGCV+LFQIKNKILKLASSGNFFGEMIQPFCKPICKANSCFNNGKCLELWRGRFICDCGFSQFVGERCNLDHSVKFNRSFSFQHSIYFKLQK+QFAFQPVIDDQHRGHKMLSLKLFKNQYIYIYLDIEYFFHVRFEQNTDWIFDEKFRMKLYNSRMYLVFIDVNIDEIRIQLFDALDKSLFERKIIQ QESNLVITDILVGDSHSHGMGWMGKYEGCLTNLMLKLDSRKFIWNEIRNFDKFPNCKEAARTTLKPNQTRSSKSKSQNQNSRAVL SKTLNGTENIPRNNDYLIYITIASIFFALLIATLLIVIIIKFKLTNQKSFSESSDKIRLTDNFNKSLNHSELPEQQQIITNSLCNSPTNSNHIYPSPIYMTFKPAYKAKSMTSSITNRSVMSLPNPTSPTLATQE+ E
Sbjct:    1 MAFIYQFLLGWMLSLISAYDQSISFSPISKSFPNSYWHGKMAILRSKSFIEEGILLKVQLNQFQGLLFYAIDSNYRTIWVEFVQEMLEISYYNFMISEANRGYFRIKDLCIPINDNKLILNFVSKNNEIIISCNGNSRTLDYTQPPEIVSENELRTFNSYMNFPVPYFNETEVHFWIGNVPSENEENLYQNIVHWKSNRLNLWGCVELFQIKNKILKLASSGNFFGEMIQPFCKPICKANSCFNNGKCLELWRGRFICDCGFSQFVGERCNLDHSVKFNRSFSFQHSIYFKLQKLQFAFQPVIDDQHRGHKMLSLKLFKNQYIYIYLDIEYFFHVRFEQNTDWIFDEKFRMKLYNSRMYLVFIDVNIDEIRIQLFDALDKSLFERKIIQPQESNLVITDILVGDSHSHGMGWMGKYEGCLTNLMLKLDSRKFIWNEIRNFDKFPNCKEAARTTLKPNQTRSSKSKSQNQNSRAVLHSKTLNGTENIPRNNDYLIYITIASIFFALLIATLLIVIIIKFKLTNQKSFSESSDKIRLTDNFNKSLNHSELPEQQQIITNSLCNSPTNSNHIYPSPIYMTFKPAYKAKSMTSSITNRSVMSLPNPTSPTLATQEDGE 614
[Close]
BLAST of SMED30023433 vs. Planmine SMEST
Match: SMESG000067632.1 (SMESG000067632.1)

HSP 1 Score: 1123.61 bits (2905), Expect = 0.000e+0
Identity = 596/603 (98.84%), Postives = 599/603 (99.34%), Query Frame = 3 
Query:  201 MLSLISAYDQXXXXXXXXXXXXXXYWHGKMAILRSKSFIEEGILLKVQLNQFQGLLFYAIDSNYRTIWVEFVQEMLEISYYNFMISEANRGYFRIKDLCIPINDNKLILNFVSKNNEIIISCNGNSRTLDYTQPPEIVSENELRTFNSYMNFPVPYFNETAVHFWIGNVPSENEENLYQNIVHWKSNRLNLWGCVKLFQIKNKILKLASSGNFFGEMIQPFCKPICKANSCFNNGKCLELWRGRFICDCGFSQFVGERCNLDHSVKFNRSFSFQHSIYFKLQKVQFAFQPVIDDQHRGHKMLSLKLFKNQYIYIYLDIEYFFHVRFEQNTDWIFDEKFRMKLYNSRMYLVFIDVNIDEIRIQLFDALDKSLFERKIIQHQESNLVITDILVGDSHSHGMGWMGKYEGCLTNLMLKLDSRKFIWNEIRNFDKFPNCKEAARTTLKPNQTXXXXXXXXXXXXXAVLLSKTLNGTENIPRNNDYLIYXXXXXXXXXXXXXXXXXXXXXKFKLTNQKSFSESSDKIRLTDNFNKSLNHSELPEQQQIITNSLCNSPTNSNHIYPSPIYMTFKPAYKAKSMTSSITNRSVMSLPNPTSPTLATQEESE 2009
            MLSLISAYDQSISFSPISKSFPNSYWHGKMAILRSKSFIEEGILLKVQLNQFQGLLFYAIDSNYRTIWVEFVQEMLEISYYNFMISEANRGYFRIKDLCIPINDNKLILNFVSKNNEIIISCNGNSRTLDYTQPPEIVSENELRTFNSYMNFPVPYFNET VHFWIGNVPSENEENLYQNIVHWKSNRLNLWGCV+LFQIKNKILKLASSGNFFGEMIQPFCKPICKANSCFNNGKCLELWRGRFICDCGFSQFVGERCNLDHSVKFNRSFSFQHSIYFKLQK+QFAFQPVIDDQHRGHKMLSLKLFKNQYIYIYLDIEYFFHVRFEQNTDWIFDEKFRMKLYNSRMYLVFIDVNIDEIRIQLFDALDKSLFERKIIQ QESNLVITDILVGDSHSHGMGWMGKYEGCLTNLMLKLDSRKFIWNEIRNFDKFPNCKEAARTTLKPNQTRSSKSKSQNQNSRAVL SKTLNGTENIPRNNDYLIYITIASIFFALLIATLLIVIIIKFKLTNQKSFSESSDKIRLTDNFNKSLNHSELPEQQQIITNSLCNSPTNSNHIYPSPIYMTFKPAYKAKSMTSSITNRSVMSLPNPTSPTLATQE+ E
Sbjct:    1 MLSLISAYDQSISFSPISKSFPNSYWHGKMAILRSKSFIEEGILLKVQLNQFQGLLFYAIDSNYRTIWVEFVQEMLEISYYNFMISEANRGYFRIKDLCIPINDNKLILNFVSKNNEIIISCNGNSRTLDYTQPPEIVSENELRTFNSYMNFPVPYFNETEVHFWIGNVPSENEENLYQNIVHWKSNRLNLWGCVELFQIKNKILKLASSGNFFGEMIQPFCKPICKANSCFNNGKCLELWRGRFICDCGFSQFVGERCNLDHSVKFNRSFSFQHSIYFKLQKLQFAFQPVIDDQHRGHKMLSLKLFKNQYIYIYLDIEYFFHVRFEQNTDWIFDEKFRMKLYNSRMYLVFIDVNIDEIRIQLFDALDKSLFERKIIQPQESNLVITDILVGDSHSHGMGWMGKYEGCLTNLMLKLDSRKFIWNEIRNFDKFPNCKEAARTTLKPNQTRSSKSKSQNQNSRAVLHSKTLNGTENIPRNNDYLIYITIASIFFALLIATLLIVIIIKFKLTNQKSFSESSDKIRLTDNFNKSLNHSELPEQQQIITNSLCNSPTNSNHIYPSPIYMTFKPAYKAKSMTSSITNRSVMSLPNPTSPTLATQEDGE 603
[Close]
BLAST of SMED30023433 vs. Planmine SMEST
Match: SMESG000013083.1 (SMESG000013083.1)

HSP 1 Score: 352.058 bits (902), Expect = 3.023e-112
Identity = 222/590 (37.63%), Postives = 340/590 (57.63%), Query Frame = 3 
Query:  288 MAILRSKSFIEEGILLKVQLNQFQGLLFYAIDSNYRTIWVEFVQEMLEISYYNFMISEANRGYFRIKDLCIPINDNKLIL----NFVSKNNEIIISCNGNSRTLDYTQPPEIVSENELRTFNSYMNFPVPYFNE----TAVHFWIGNVPSENEENLYQNIVHWKSNRLNLWGCVKLFQIKNKILKLASSGNFFGEMIQPFCKPICKANSCFNNGKCLELWRGRFICDCGFSQFVGERCNLDHSVKFNRSFSFQHSIYFKLQKVQFAFQPVIDDQHRGHKMLSLKLFKNQYIYIYLDIEYFFHVRFEQNTDWIFDEKFRMKLYNSRMYLVFIDVNIDEIRIQLFDALDKSLFERKIIQHQESNLV--ITDILVGDSHSHGMGWMGKYEGCLTNLMLKLDSRKFIWNEIRNFDKFPNCK------EAARTTLKPNQTXXXXXXXXXXXXXAVLLSKTLNGTENIPRNNDYLIYXXXXXXXXXXXXXXXXXXXXXKFKLTNQKSFSESSDKIRLTDNFNKSLNHSELPEQQQIITNSLCNSPTNSNHIYPSPIYMTFKPAYKAKSMTSSITNRSVMSLPNPTSPTLATQEESE 2009
            + I +++ F+EEGI +K+ LN++QGLLFY IDSNYRT+W+EF +  L I+YYNF I+E  +  F+I  L   + ++K ++          N++ I CN   + +DY +P   + E+ L+ F      P+P+F+     T +H WIGN+P  +  N +QNI   K+ RLNL GCV+ F I N  +KLA+SG   GEMI+PFCKPIC  +SCFN G+C ELWRG + CDC +SQF+G RC L++S+K + +FS   + +  L++++F+F+P    ++ G K+LSLK    QY  +Y+D     H R   + D   D  F  KL +++ ++   +V ++EI++++F   + +L E + I   E ++   +  ILVGD H      M +++GCLTN +L+ ++++FI N  R    +P C+      EA  ++ KPN+      K Q        + KT N +EN      Y+I   ++S    ++   +L   I   K  +++SF             N  L+H+ L E+Q    N+   + +   H+YPSPI MTFKPAYKAKSM SSITN S++SLP   SPTLA  E+ E
Sbjct:    2 VMIGKNRFFLEEGINMKINLNKYQGLLFYIIDSNYRTLWLEFSENFLVINYYNFKINELEKHTFKISFL---LGNHKNVIEADFGLSYTQNKLRIYCNDKFQIVDYVKPLYPIPESNLKQFIETFTMPIPFFDNSNETTEIHVWIGNIPKTS--NKFQNIAKLKTIRLNLLGCVEQFSITNLNMKLATSGEHVGEMIEPFCKPICNTSSCFNQGQCYELWRGNYECDCEYSQFIGRRCELEYSIKLDGTFSVTENHFISLKRLRFSFRPNTAGEYDGFKLLSLKFKDQQYFVMYIDSNSLLHGRLIYSNDLNIDVYFTYKLKHNQSFIFLAEVRLNEIQMKIFYFQNYTLIETQTISFGEISIFFNVRQILVGDLHIPFFYSMKRFKGCLTNFVLQSETKQFILNTSRESTNYPPCQQIAGYNEAWNSSYKPNKFTKELPKIQ-------FIGKTRNISENKNNYTAYIIISVVSSFSLLVVGIIILFCKIHSRKEKHKESFQVLE---------NNRLHHNYLKEKQFQSLNNGDVTDSIPCHVYPSPICMTFKPAYKAKSMCSSITNGSLLSLPLSISPTLAVPEDRE 570
[Close]
BLAST of SMED30023433 vs. Planmine SMEST
Match: SMESG000013083.1 (SMESG000013083.1)

HSP 1 Score: 351.673 bits (901), Expect = 3.968e-112
Identity = 220/588 (37.41%), Postives = 339/588 (57.65%), Query Frame = 3 
Query:  294 ILRSKSFIEEGILLKVQLNQFQGLLFYAIDSNYRTIWVEFVQEMLEISYYNFMISEANRGYFRIKDLCIPINDNKLIL----NFVSKNNEIIISCNGNSRTLDYTQPPEIVSENELRTFNSYMNFPVPYFNE----TAVHFWIGNVPSENEENLYQNIVHWKSNRLNLWGCVKLFQIKNKILKLASSGNFFGEMIQPFCKPICKANSCFNNGKCLELWRGRFICDCGFSQFVGERCNLDHSVKFNRSFSFQHSIYFKLQKVQFAFQPVIDDQHRGHKMLSLKLFKNQYIYIYLDIEYFFHVRFEQNTDWIFDEKFRMKLYNSRMYLVFIDVNIDEIRIQLFDALDKSLFERKIIQHQESNLV--ITDILVGDSHSHGMGWMGKYEGCLTNLMLKLDSRKFIWNEIRNFDKFPNCK------EAARTTLKPNQTXXXXXXXXXXXXXAVLLSKTLNGTENIPRNNDYLIYXXXXXXXXXXXXXXXXXXXXXKFKLTNQKSFSESSDKIRLTDNFNKSLNHSELPEQQQIITNSLCNSPTNSNHIYPSPIYMTFKPAYKAKSMTSSITNRSVMSLPNPTSPTLATQEESE 2009
            I +++ F+EEGI +K+ LN++QGLLFY IDSNYRT+W+EF +  L I+YYNF I+E  +  F+I  L   + ++K ++          N++ I CN   + +DY +P   + E+ L+ F      P+P+F+     T +H WIGN+P  +  N +QNI   K+ RLNL GCV+ F I N  +KLA+SG   GEMI+PFCKPIC  +SCFN G+C ELWRG + CDC +SQF+G RC L++S+K + +FS   + +  L++++F+F+P    ++ G K+LSLK    QY  +Y+D     H R   + D   D  F  KL +++ ++   +V ++EI++++F   + +L E + I   E ++   +  ILVGD H      M +++GCLTN +L+ ++++FI N  R    +P C+      EA  ++ KPN+          +  +   + KT N +EN      Y+I   ++S    ++   +L   I   K  +++SF             N  L+H+ L E+Q    N+   + +   H+YPSPI MTFKPAYKAKSM SSITN S++SLP   SPTLA  E+ E
Sbjct:    2 IGKNRFFLEEGINMKINLNKYQGLLFYIIDSNYRTLWLEFSENFLVINYYNFKINELEKHTFKISFL---LGNHKNVIEADFGLSYTQNKLRIYCNDKFQIVDYVKPLYPIPESNLKQFIETFTMPIPFFDNSNETTEIHVWIGNIPKTS--NKFQNIAKLKTIRLNLLGCVEQFSITNLNMKLATSGEHVGEMIEPFCKPICNTSSCFNQGQCYELWRGNYECDCEYSQFIGRRCELEYSIKLDGTFSVTENHFISLKRLRFSFRPNTAGEYDGFKLLSLKFKDQQYFVMYIDSNSLLHGRLIYSNDLNIDVYFTYKLKHNQSFIFLAEVRLNEIQMKIFYFQNYTLIETQTISFGEISIFFNVRQILVGDLHIPFFYSMKRFKGCLTNFVLQSETKQFILNTSRESTNYPPCQQIAGYNEAWNSSYKPNK-------FTKELPKIQFIGKTRNISENKNNYTAYIIISVVSSFSLLVVGIIILFCKIHSRKEKHKESFQVLE---------NNRLHHNYLKEKQFQSLNNGDVTDSIPCHVYPSPICMTFKPAYKAKSMCSSITNGSLLSLPLSISPTLAVPEDRE 568
[Close]
BLAST of SMED30023433 vs. Planmine SMEST
Match: SMESG000042896.1 (SMESG000042896.1)

HSP 1 Score: 58.9214 bits (141), Expect = 3.447e-9
Identity = 64/245 (26.12%), Postives = 100/245 (40.82%), Query Frame = 3 
Query:  315 IEEGILLKVQLNQFQGLLFYAIDSNYRTIWVEFVQEMLEISYYNFMISEANRGYFRIKDLCIPIND----NKLILNFVSKNNEIIISCN-GNSRTLDYTQPPEIVSENELRTFNSYMNFPVPYFNE-TAVHFWIGNVPSENEENLYQNIVHWKSNRLNLWGCVKLFQIKNKILKLASSGNFFGEMIQPFCKPICKANSCFNNGKCLELWRGRFICDCGFSQFVGERCNLDHSVKFNRSFSFQHSI 1031
            I E   L V L++F GLL Y+ID+ Y  + +   +         F    + +   R+ DLC          +L+ +F    + I I CN G+     +T         E+R   +  ++P+P F+  T  H W  N+P                N   L GC+K    K + +KL   G      +   C+  C+  +C N+G CLE +  +  C C  +   GE CN   S   +R F+F   I
Sbjct:   47 ILEKTELTVYLSRFNGLLLYSIDNQYNQLVINLQESKPPCMKVEF---RSEKYMIRVDDLCKCKYQYCLYEQLVFSF-EITDTINIMCNFGSVNKHSFT---------EIRRIKA--DYPIPDFDRGTFAHLWFINLPYHTSR-----FGQTYPNPEFLTGCIKKVTYKGETIKLNRIG------VNRTCETKCQPGTC-NDGNCLEAFNSQIKCFCNDTLLNGEFCNASLSGFGSRKFTFYSKI 264
The following BLAST results are available for this feature:
BLAST of SMED30023433 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
NRXN18.501e-732.00neurexin 1 [Source:HGNC Symbol;Acc:HGNC:8008][more]
NRXN11.016e-632.00neurexin 1 [Source:HGNC Symbol;Acc:HGNC:8008][more]
NRXN11.045e-632.00neurexin 1 [Source:HGNC Symbol;Acc:HGNC:8008][more]
NRXN11.542e-632.00neurexin 1 [Source:HGNC Symbol;Acc:HGNC:8008][more]
NRXN13.406e-632.00neurexin 1 [Source:HGNC Symbol;Acc:HGNC:8008][more]
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BLAST of SMED30023433 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023433 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023433 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
nrxn3b2.073e-628.41neurexin 3b [Source:NCBI gene;Acc:570698][more]
nrxn3b2.243e-628.41neurexin 3b [Source:NCBI gene;Acc:570698][more]
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BLAST of SMED30023433 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023433 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Nrxn17.465e-732.00neurexin I [Source:MGI Symbol;Acc:MGI:1096391][more]
Nrxn19.479e-732.00neurexin I [Source:MGI Symbol;Acc:MGI:1096391][more]
Nrxn12.338e-632.00neurexin I [Source:MGI Symbol;Acc:MGI:1096391][more]
Nrxn12.339e-632.00neurexin I [Source:MGI Symbol;Acc:MGI:1096391][more]
Nrxn12.339e-632.00neurexin I [Source:MGI Symbol;Acc:MGI:1096391][more]
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BLAST of SMED30023433 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 2
Match NameE-valueIdentityDescription
sp|Q9ULB1|NRX1A_HUMAN7.406e-632.00Neurexin-1 OS=Homo sapiens OX=9606 GN=NRXN1 PE=1 S... [more]
sp|Q9DDD0|NRX1A_CHICK9.428e-632.00Neurexin-1 (Fragment) OS=Gallus gallus OX=9031 GN=... [more]
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BLAST of SMED30023433 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A158QKH52.308e-726.14Uncharacterized protein OS=Haemonchus placei OX=62... [more]
A0A0B7ATQ37.247e-725.28Uncharacterized protein OS=Arion vulgaris OX=10286... [more]
A0A2C9LN941.270e-630.63Uncharacterized protein OS=Biomphalaria glabrata O... [more]
W2TTR52.503e-627.52EGF-like domain protein OS=Necator americanus OX=5... [more]
A0A433TUD43.165e-631.63Uncharacterized protein OS=Elysia chlorotica OX=18... [more]
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BLAST of SMED30023433 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 4
Match NameE-valueIdentityDescription
ENSAMXT00000021549.22.410e-723.33pep primary_assembly:Astyanax_mexicanus-2.0:14:799... [more]
ENSAMXT00000043111.13.644e-723.29pep primary_assembly:Astyanax_mexicanus-2.0:14:799... [more]
ENSAMXT00000050294.14.598e-723.29pep primary_assembly:Astyanax_mexicanus-2.0:14:799... [more]
nrxn3b6.330e-628.41neurexin 3b [Source:NCBI gene;Acc:570698][more]
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BLAST of SMED30023433 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 2
Match NameE-valueIdentityDescription
ENSPMAT00000002142.16.196e-825.84pep scaffold:Pmarinus_7.0:GL476386:209124:506099:1... [more]
ENSPMAT00000004299.13.208e-625.00pep scaffold:Pmarinus_7.0:GL477235:143017:163597:1... [more]
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BLAST of SMED30023433 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023433 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023433 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 3
Match NameE-valueIdentityDescription
nrxn3b8.718e-729.55neurexin-3b [Source:NCBI gene;Acc:101156134][more]
nrxn3b8.781e-729.55neurexin-3b [Source:NCBI gene;Acc:101156134][more]
nrxn3b8.788e-729.55neurexin-3b [Source:NCBI gene;Acc:101156134][more]
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BLAST of SMED30023433 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000067632.10.000e+098.86SMESG000067632.1[more]
SMESG000067632.10.000e+098.84SMESG000067632.1[more]
SMESG000013083.13.023e-11237.63SMESG000013083.1[more]
SMESG000013083.13.968e-11237.41SMESG000013083.1[more]
SMESG000042896.13.447e-926.12SMESG000042896.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30023433 ID=SMED30023433|Name=SMED30023433|organism=Schmidtea mediterranea sexual|type=transcript|length=2216bp
TTTTTTTTAATTTCGTAAGATTTTTAACTGAATTACAATATTACAAATTT
TAAACAAAATTTACAATAATTAAACTGAAATTAATTGGTTACAATTCAAC
CAATCAAATTTATATTAAAAATAGATATCAATTGAAGTCTACTGTTTTCG
GCACAATATTTCAAACGATGGCATTTATATATCAATTTCTATTGGGATGG
ATGTTATCTTTAATATCAGCCTATGATCAATCAATCAGTTTCTCTCCGAT
ATCAAAATCGTTTCCAAATTCATATTGGCATGGTAAAATGGCAATTTTGA
GAAGTAAATCATTCATTGAAGAAGGAATTCTACTTAAAGTGCAACTAAAT
CAATTTCAAGGACTCCTATTCTATGCGATAGATAGTAACTATCGAACCAT
TTGGGTAGAATTCGTACAAGAAATGTTAGAAATCAGTTATTACAATTTCA
TGATTTCTGAAGCAAACAGGGGCTATTTCAGAATTAAAGACTTATGTATT
CCTATTAATGACAACAAATTAATTTTAAATTTTGTCAGCAAAAATAACGA
AATAATTATCAGTTGCAATGGAAATAGTCGAACTCTGGATTATACTCAAC
CTCCAGAAATTGTATCAGAAAATGAGTTGAGAACATTCAATAGTTATATG
AATTTTCCAGTACCTTATTTCAATGAGACTGCGGTACATTTCTGGATAGG
AAACGTTCCTAGTGAAAACGAAGAGAACCTATACCAAAACATTGTACACT
GGAAATCGAATCGTTTAAATCTGTGGGGATGTGTCAAACTCTTTCAAATA
AAAAATAAAATTTTGAAGCTAGCTTCTTCCGGAAATTTTTTTGGAGAAAT
GATTCAACCGTTTTGCAAACCAATTTGTAAGGCTAATTCATGTTTTAATA
ATGGTAAATGCCTCGAGTTATGGAGAGGACGATTTATTTGTGATTGTGGA
TTTTCTCAGTTTGTGGGAGAGCGCTGCAATTTGGATCACTCGGTTAAATT
TAACAGAAGCTTTTCATTTCAGCACTCAATATACTTTAAATTGCAGAAAG
TACAATTCGCGTTTCAACCAGTCATTGATGATCAGCACAGGGGTCACAAA
ATGCTTTCCCTCAAATTGTTTAAAAATCAATATATTTACATTTATCTTGA
CATTGAGTACTTTTTTCATGTCCGTTTTGAACAAAATACTGATTGGATTT
TTGATGAAAAATTCAGAATGAAATTGTACAATAGTAGAATGTACCTGGTA
TTTATTGATGTAAATATCGACGAAATTCGAATACAACTTTTCGATGCACT
CGATAAAAGTCTTTTCGAAAGGAAAATTATTCAACACCAAGAAAGCAATC
TTGTCATCACGGATATATTAGTAGGCGATTCTCATTCTCACGGCATGGGT
TGGATGGGTAAATACGAAGGATGTCTAACAAATCTCATGCTAAAACTAGA
TTCAAGAAAGTTTATTTGGAACGAAATTCGAAATTTTGATAAATTTCCAA
ATTGTAAAGAGGCGGCTAGAACAACTTTAAAACCGAACCAAACTCGTTCT
TCCAAATCAAAGAGTCAAAATCAAAATTCTCGAGCCGTGCTTCTTAGTAA
AACCTTGAACGGCACTGAAAATATTCCAAGAAATAATGATTACTTGATAT
ACATCACAATAGCAAGCATTTTTTTTGCATTGTTGATAGCCACATTATTA
ATTGTAATAATTATCAAATTCAAATTAACAAATCAAAAGTCATTTTCAGA
GTCATCAGATAAAATTAGACTCACAGATAATTTCAACAAATCTCTGAATC
ATTCGGAACTGCCAGAACAACAACAAATAATCACCAACAGCCTTTGCAAT
TCACCCACAAATTCCAATCATATTTACCCCTCACCAATATATATGACCTT
CAAGCCAGCATATAAAGCAAAAAGTATGACCTCTTCCATAACCAATAGGT
CAGTAATGTCATTGCCGAACCCCACATCCCCAACACTAGCTACTCAAGAA
GAGTCTGAAATGGAGAATTCTAACGACGTCTCCTTTGACAGCAGTTTCAC
CACGCCAAAGATTTTATACAAACCGGGCAAATCTATGTACAAAAAATGAA
TTGCATTTTTAATTTATCGCTATATAAAATTTAGATCTGTTATTTTTGTC
ATTTTTCTGTTTGAACATAATTTTGATTATTTTTATGTTGTAACGAATAT
ATTTCTGAAAATGAAA
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protein sequence of SMED30023433-orf-1

>SMED30023433-orf-1 ID=SMED30023433-orf-1|Name=SMED30023433-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=644bp
MAFIYQFLLGWMLSLISAYDQSISFSPISKSFPNSYWHGKMAILRSKSFI
EEGILLKVQLNQFQGLLFYAIDSNYRTIWVEFVQEMLEISYYNFMISEAN
RGYFRIKDLCIPINDNKLILNFVSKNNEIIISCNGNSRTLDYTQPPEIVS
ENELRTFNSYMNFPVPYFNETAVHFWIGNVPSENEENLYQNIVHWKSNRL
NLWGCVKLFQIKNKILKLASSGNFFGEMIQPFCKPICKANSCFNNGKCLE
LWRGRFICDCGFSQFVGERCNLDHSVKFNRSFSFQHSIYFKLQKVQFAFQ
PVIDDQHRGHKMLSLKLFKNQYIYIYLDIEYFFHVRFEQNTDWIFDEKFR
MKLYNSRMYLVFIDVNIDEIRIQLFDALDKSLFERKIIQHQESNLVITDI
LVGDSHSHGMGWMGKYEGCLTNLMLKLDSRKFIWNEIRNFDKFPNCKEAA
RTTLKPNQTRSSKSKSQNQNSRAVLLSKTLNGTENIPRNNDYLIYITIAS
IFFALLIATLLIVIIIKFKLTNQKSFSESSDKIRLTDNFNKSLNHSELPE
QQQIITNSLCNSPTNSNHIYPSPIYMTFKPAYKAKSMTSSITNRSVMSLP
NPTSPTLATQEESEMENSNDVSFDSSFTTPKILYKPGKSMYKK*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000034epidermis
PLANA:0000106ciliated epithelial cell
PLANA:0003116parenchymal cell
Vocabulary: INTERPRO
TermDefinition
IPR013320ConA-like_dom_sf
IPR000742EGF-like_dom
IPR024068Rotavirus_NSP2_N
Vocabulary: molecular function
TermDefinition
GO:0005515protein binding
GO:0016817hydrolase activity, acting on acid anhydrides
GO:0033130acetylcholine receptor binding
GO:0046872metal ion binding
GO:0050839cell adhesion molecule binding
GO:0005246calcium channel regulator activity
GO:0005509calcium ion binding
GO:0097109neuroligin family protein binding
Vocabulary: biological process
TermDefinition
GO:0007268chemical synaptic transmission
GO:0007269neurotransmitter secretion
GO:0007416synapse assembly
GO:0007612learning
GO:0030198extracellular matrix organization
GO:0030534adult behavior
GO:0050885neuromuscular process controlling balance
GO:0051290protein heterotetramerization
GO:0051965positive regulation of synapse assembly
GO:0006904vesicle docking involved in exocytosis
GO:0007155cell adhesion
GO:0051968positive regulation of synaptic transmission, glutamatergic
GO:0060134prepulse inhibition
GO:0065009regulation of molecular function
GO:0090129positive regulation of synapse maturation
GO:0097104postsynaptic membrane assembly
GO:0097116gephyrin clustering involved in postsynaptic density assembly
GO:0097118neuroligin clustering involved in postsynaptic membrane assembly
GO:0097119postsynaptic density protein 95 clustering
GO:2000463positive regulation of excitatory postsynaptic potential
GO:2000821regulation of grooming behavior
Vocabulary: cellular component
TermDefinition
GO:0009986cell surface
GO:0016020membrane
GO:0016021integral component of membrane
GO:0030054cell junction
GO:0031965nuclear membrane
GO:0031982vesicle
GO:0042734presynaptic membrane
GO:0043025neuronal cell body
GO:0045202synapse
GO:0005783endoplasmic reticulum
GO:0005886plasma membrane
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3DG3DSA:2.10.25.10coord: 234..272
e-value: 1.2E-8
score: 36.7
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 598..624
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 452..479
NoneNo IPR availableCDDcd00054EGF_CAcoord: 240..270
e-value: 0.00729726
score: 32.6086
NoneNo IPR availableSIGNALP_EUKSignalP-noTMSignalP-noTMcoord: 1..18
score: 0.813
NoneNo IPR availableSUPERFAMILYSSF57196EGF/Laminincoord: 236..272
NoneNo IPR availableTMHMMTMhelixcoord: 494..516
IPR000742EGF-like domainPROSITEPS50026EGF_3coord: 234..271
score: 11.235