SMED30023417

Overview
NameSMED30023417
Smed IDSMED30023417
Length (bp)3050
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30023417 (SMED30023417) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30023417 (SMED30023417) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30023417 (SMED30023417) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30023417 (SMED30023417) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30023417

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 2

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30023417SMESG000027258.1 SmedASXL_011760SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
X2 cellSMED30023417SMESG000027258.1 SmedASXL_011760SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30023417 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.000078:41303..48161 +14978
Homology
[Close]
BLAST of SMED30023417 vs. TrEMBL
Match: C3Y2Y3 (Uncharacterized protein OS=Branchiostoma floridae OX=7739 GN=BRAFLDRAFT_124611 PE=4 SV=1)

HSP 1 Score: 62.003 bits (149), Expect = 7.311e-6
Identity = 73/271 (26.94%), Postives = 115/271 (42.44%), Query Frame = 2 
Query:   92 NIVSLQHDIVKHKDTISE----AFHLF---PPSRPALLDIFCDIIVDKYVGSYIHQRNNYTNELEEIITSLTNEVNIILDKEFESNDYVLTAVNTKVSHYSGYGFVVYACQWVLQVLAKITMKYDQILAFNEPLSVNDKLK-WTQVPVIINLLDILCKTLKSKMVGHET---VSIIMKNVAQHRNHLLWVIKFIGERHPSGIVDKIIDCILSEF-------IPTQEYEKLQSCMVVLSSLGAHHARQLQSALYKKLKAAI-EGSFE--TVP 841
              V  +H    HK T+ E    A  L    P +R A+L+ F   I D+ V S+I Q          I +  +  V   LD   +    VL     KV+    +  ++ A  W + +L +I+ KY          S+N+ L+ W        L+DI  + L + M G      V  ++    QH  H  WV+  IG   P+ I+ +++ C L +F       +      KL S + +L  L AHHA+ ++ AL +    ++ EG  E  TVP
Sbjct:   26 TFVQGKHATFGHKLTLEEHARSALSLLRNVPAARSAVLE-FLQGIFDEAVNSHILQ----------IESEGSGTVGPNLDPVIQEVCSVLLGF-VKVN-PQAWAPLISA--WSIDLLGQISTKYAGRRGVPHSSSLNELLQLWMTCKATRTLMDIYTQCLAA-MTGSSADLCVDALLDTSVQHSPHFDWVVAHIGSSFPNTIITRVLSCGLKDFCSHSSSQVTESHVPKLASVVGILGHLAAHHAQDIREALLQLFHVSLSEGKQEQYTVP 280
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BLAST of SMED30023417 vs. TrEMBL
Match: A0A224XJD9 (Putative integrator complex subunit 5-like isoform x1 (Fragment) OS=Panstrongylus lignarius OX=156445 PE=4 SV=1)

HSP 1 Score: 62.003 bits (149), Expect = 8.485e-6
Identity = 61/262 (23.28%), Postives = 115/262 (43.89%), Query Frame = 2 
Query:  158 FPPSRPALLDIFCDIIVDKYVGSYIHQRN-NYTNELEEIITSLTNEVNIILDKEFESNDYVLTAVNTKVSHYSGYGFVVYACQWVLQVLAKITMKYDQILAFNEPLSVNDKLK-WTQVPVIINLLDILCKTLKSKMVGHET----VSIIMKNVAQHRNHLLWVIKFIGERHPSGIVDKIIDCILSEFI--P--TQEYEKLQSCMVVLSSLGAHHARQLQSALYKKLKAAIEGSFETVPKQKIINYQTLIYFTLLLASRSDVF 913
             P SR A+ +         Y+G+   +   N+  ELE    +L N  N   D   E    +  ++NT +++     + V   +W  + L  ++ KY          S++D L+ W        LLDI    LK +++G++     +S ++    +H  H  WV+ +IG  +P  +V +++ C L EF   P    +  KL+S + +L  L   H  +++ A+      ++E +     ++ I    + + + L LAS + V 
Sbjct:   39 LPASRDAVFE---------YLGTVFEKSTLNHMQELENRNKALNNSSNSFDDSIIEE---INASLNTFLTNQP-LAWAVIVSKWAFEHLGSLSSKYSSRGKSPVNGSLSDSLQQWMSCNATSALLDITTNCLK-RLIGNDEARNCISFLLDTCVKHSPHFDWVVAYIGSCYPEMVVIRVLSCGLQEFSCNPGNAAKIPKLKSVVGILIHLSGSHRAEIKKAMSDLFNWSMEENSRNEDREIIREKNSAVPYLLQLASLAPVL 286
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BLAST of SMED30023417 vs. Planmine SMEST
Match: SMESG000027258.1 (SMESG000027258.1)

HSP 1 Score: 1961.81 bits (5081), Expect = 0.000e+0
Identity = 978/980 (99.80%), Postives = 979/980 (99.90%), Query Frame = 2 
Query:   56 MTSVELSWKKLVNIVSLQHDIVKHKDTISEAFHLFPPSRPALLDIFCDIIVDKYVGSYIHQRNNYTNELEEIITSLTNEVNIILDKEFESNDYVLTAVNTKVSHYSGYGFVVYACQWVLQVLAKITMKYDQILAFNEPLSVNDKLKWTQVPVIINLLDILCKTLKSKMVGHETVSIIMKNVAQHRNHLLWVIKFIGERHPSGIVDKIIDCILSEFIPTQEYEKLQSCMVVLSSLGAHHARQLQSALYKKLKAAIEGSFETVPKQKIINYQTLIYFTLLLASRSDVFELTNASISMIGRIICQFTPKQLEQFFLHQNFGDNILRSYYHMTLNILLNSHDLEIGRVLEFIIHHTHSTITGRTGVSCCSANFSQMCMNIIANVSVKLLIQCHSAGLSKPSNNRTMALAILNYVMQHLEFWIQRVVKDGSCVAAKLLSCLVLNDFKSIGDDLLLTIVTNDPQTPTEVNGHKKALVLFFRLVDEFESVSYNILEEVTTRSLCVAQSYQPSRQMRYLSTLYSMLVYEARMQSQRRRLARAINSNIDXXXXXXXTNKXXXXXXXXXXXXXXXXXXXXEKYSVPISSPYLCKTDTRFKAWPIFVASRVVRCISVMLSILIESLNANNPSSVILIRRLTDFLVNFLRTTDPRHTISAVQIIFREFLRSSWIDTNNRFVFKKTLKPGWFNGKLVHDYPPTDSKTGRLNNTNQSPIIRNRYSPIQEKHWEVLIMTLCHFHRQHDVNSTSAYQVIQWICRQCLSPSYYLLINADLNTIPKDLNYITIFWKRFNQSELFWGLFYMLSRKAPQTFQPPYQTLEALMSACQHLWSTSDCPRKRLIRNVPDSEIFISDSHDPIILHKQRFYELINSNSNLYRATVFLFECLSSQMALTDELKLMLPYLSCSEVQTILFHLAYIWNKKVQKPPTVPAPVNEIDADGNVVSVSMVPIEDTQPKNTFSWETLLPALVHARIHFKSLYDQFCRLLFISCE 2995
            MTSVELSWKKLVNIVSLQHDIVKHKDTISEAFHLFPPSRPALLDIFCDIIVDKYVGSYIHQRNNYTNELEEIITSLTNEVNIILDKEFESNDYVLTAVNTKVSHYSGYGFVVYACQWVLQVLAKITMKYDQILAFNEPLSVNDKLKWTQVPVIINLLDILCKTLKSKMVGHETVSIIMKNVAQHRNHLLWVIKFIGERHPSGIVDKIIDCILSEFIPTQEYEKLQSCMVVLSSLGAHHARQLQSALYKKLKAAIEGSFETVPKQKIINYQTLIYFTLLLASRSDVFELTNASISMIGRIICQFTPKQLEQFFLHQNFGDNILRSYYHMTLNILLNSHDLEIGRVLEF+IHHTHSTITGRTGVSCCSANFSQMCMNIIANVSVKLLIQCHSAGLSKPSNNRTMALAILNYVMQHLEFWIQRVVKDGSCVAAKLLSCLVLNDFKSIGDDLLLTIVTNDPQTPTEVNGHKKALVLFFRLVDEFESVSYNILEEVTTRSLCVAQSYQPSRQMRYLSTLYSMLVYEARMQSQRRRLARAINSNIDLLLLLVVTNKSLPIIKISISILSKIDVVSDEKYSVPISSPYLCKTDTRFKAWPIFVASRVVRCISVMLSILIESLNANNPSSVILIRRLTDFLVNFLRTTDPRHTISAVQIIFREFLRSSWIDTNNRFVFKKTLKPGWFNGKLVHDYPPTDSKTGRLNNTNQSPIIRNRYSPIQEKHWEVLIMTLCHFHRQHDVNSTSAYQVIQWICRQCLSPSYYLLINADLNTIPKDLNYITIFWKRFNQSELFW LFYMLSRKAPQTFQPPYQTLEALMSACQHLWSTSDCPRKRLIRNVPDSEIFISDSHDPIILHKQRFYELINSNSNLYRATVFLFECLSSQMALTDELKLMLPYLSCSEVQTILFHLAYIWNKKVQKPPTVPAPVNEIDADGNVVSVSMVPIEDTQPKNTFSWETLLPALVHARIHFKSLYDQFCRLLFISCE
Sbjct:    1 MTSVELSWKKLVNIVSLQHDIVKHKDTISEAFHLFPPSRPALLDIFCDIIVDKYVGSYIHQRNNYTNELEEIITSLTNEVNIILDKEFESNDYVLTAVNTKVSHYSGYGFVVYACQWVLQVLAKITMKYDQILAFNEPLSVNDKLKWTQVPVIINLLDILCKTLKSKMVGHETVSIIMKNVAQHRNHLLWVIKFIGERHPSGIVDKIIDCILSEFIPTQEYEKLQSCMVVLSSLGAHHARQLQSALYKKLKAAIEGSFETVPKQKIINYQTLIYFTLLLASRSDVFELTNASISMIGRIICQFTPKQLEQFFLHQNFGDNILRSYYHMTLNILLNSHDLEIGRVLEFMIHHTHSTITGRTGVSCCSANFSQMCMNIIANVSVKLLIQCHSAGLSKPSNNRTMALAILNYVMQHLEFWIQRVVKDGSCVAAKLLSCLVLNDFKSIGDDLLLTIVTNDPQTPTEVNGHKKALVLFFRLVDEFESVSYNILEEVTTRSLCVAQSYQPSRQMRYLSTLYSMLVYEARMQSQRRRLARAINSNIDLLLLLVVTNKSLPIIKISISILSKIDVVSDEKYSVPISSPYLCKTDTRFKAWPIFVASRVVRCISVMLSILIESLNANNPSSVILIRRLTDFLVNFLRTTDPRHTISAVQIIFREFLRSSWIDTNNRFVFKKTLKPGWFNGKLVHDYPPTDSKTGRLNNTNQSPIIRNRYSPIQEKHWEVLIMTLCHFHRQHDVNSTSAYQVIQWICRQCLSPSYYLLINADLNTIPKDLNYITIFWKRFNQSELFWSLFYMLSRKAPQTFQPPYQTLEALMSACQHLWSTSDCPRKRLIRNVPDSEIFISDSHDPIILHKQRFYELINSNSNLYRATVFLFECLSSQMALTDELKLMLPYLSCSEVQTILFHLAYIWNKKVQKPPTVPAPVNEIDADGNVVSVSMVPIEDTQPKNTFSWETLLPALVHARIHFKSLYDQFCRLLFISCE 980
The following BLAST results are available for this feature:
BLAST of SMED30023417 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023417 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023417 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023417 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023417 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023417 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023417 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023417 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 2
Match NameE-valueIdentityDescription
C3Y2Y37.311e-626.94Uncharacterized protein OS=Branchiostoma floridae ... [more]
A0A224XJD98.485e-623.28Putative integrator complex subunit 5-like isoform... [more]
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BLAST of SMED30023417 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023417 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023417 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023417 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023417 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023417 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000027258.10.000e+099.80SMESG000027258.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30023417 ID=SMED30023417|Name=SMED30023417|organism=Schmidtea mediterranea sexual|type=transcript|length=3050bp
AATAACTTTAATATATTGAATTAAATTGTAATTAAATAAAATATATTTTG
GTGTCATGACTTCTGTTGAATTATCTTGGAAAAAATTAGTAAATATAGTT
TCCCTTCAACATGATATTGTCAAGCATAAAGATACGATATCTGAGGCTTT
TCATCTTTTCCCGCCTTCTCGACCAGCTCTATTAGATATTTTTTGTGATA
TTATCGTAGATAAATATGTTGGATCTTATATTCACCAACGAAATAACTAC
ACAAACGAATTAGAAGAAATCATAACATCATTGACAAACGAAGTAAATAT
CATTCTTGATAAAGAATTTGAATCAAATGATTATGTTCTGACAGCAGTTA
ATACTAAAGTTTCTCATTACTCAGGTTATGGATTTGTTGTTTATGCCTGC
CAATGGGTATTGCAAGTTCTTGCTAAGATAACAATGAAATATGATCAAAT
TTTGGCATTTAATGAACCTTTGTCCGTTAATGATAAATTAAAATGGACTC
AAGTACCAGTTATTATAAATCTTTTGGATATCTTATGTAAAACTCTAAAA
AGCAAAATGGTTGGACATGAAACTGTGTCGATCATAATGAAAAACGTTGC
CCAACATCGGAACCATTTGCTATGGGTGATAAAGTTCATCGGAGAAAGGC
ACCCGAGTGGGATAGTTGATAAGATAATCGATTGCATCCTCAGCGAGTTT
ATCCCGACCCAGGAATATGAGAAACTTCAATCGTGCATGGTCGTGTTGAG
CAGTCTCGGTGCTCACCATGCACGCCAGTTACAGTCAGCCTTGTATAAAA
AATTAAAGGCTGCCATTGAAGGCTCATTTGAAACCGTCCCGAAACAAAAG
ATAATCAACTACCAGACTTTAATATACTTTACTCTGCTTCTAGCGTCCCG
GAGTGACGTTTTCGAGTTGACCAACGCCTCGATCTCGATGATTGGACGCA
TTATCTGTCAATTCACGCCGAAACAACTTGAACAGTTTTTCTTACATCAG
AATTTCGGGGACAATATTTTGCGCAGTTATTACCACATGACGCTGAATAT
CTTGCTGAATTCACATGATCTTGAGATAGGCCGCGTCCTCGAGTTCATAA
TTCACCATACCCACTCGACAATCACTGGCCGGACTGGTGTGAGTTGCTGC
AGCGCAAACTTCAGTCAGATGTGCATGAACATAATTGCCAATGTGTCGGT
TAAGCTGCTAATACAATGCCACTCGGCTGGGCTGTCGAAGCCATCTAATA
ATCGGACGATGGCACTTGCCATTTTGAATTACGTGATGCAGCATCTGGAA
TTCTGGATCCAGAGAGTAGTTAAGGATGGGTCTTGTGTAGCGGCTAAGCT
ACTGTCATGTCTCGTTCTCAATGACTTCAAATCGATAGGAGACGATTTGC
TTTTGACCATTGTAACCAACGATCCCCAGACCCCCACTGAAGTAAATGGT
CATAAAAAAGCACTAGTTCTCTTTTTCCGACTGGTTGACGAATTCGAGTC
TGTCAGCTATAACATCCTAGAAGAAGTCACAACGCGCAGTTTGTGTGTCG
CGCAATCATATCAGCCGTCACGTCAAATGCGCTATTTATCGACGCTTTAT
TCCATGCTCGTCTACGAGGCTCGCATGCAATCTCAGCGCCGGCGATTAGC
CAGAGCTATAAACTCCAATATCGATTTGCTGCTGTTACTTGTCGTGACCA
ACAAATCTTTACCTATCATCAAGATTTCCATTTCTATTCTCTCCAAAATC
GATGTCGTGAGCGATGAGAAATATTCCGTGCCGATATCGTCACCGTATCT
ATGCAAAACCGACACGAGATTTAAAGCCTGGCCTATTTTCGTAGCCTCCC
GAGTCGTCCGATGCATTTCCGTTATGCTATCGATTTTAATCGAATCCCTA
AACGCTAACAATCCCTCCTCTGTCATTCTTATTCGACGTCTTACAGATTT
CCTAGTAAACTTCCTGCGTACGACTGATCCCAGGCACACGATCTCAGCAG
TCCAGATCATTTTCCGAGAATTCCTTCGCTCATCATGGATCGATACAAAC
AATCGATTCGTGTTCAAAAAAACCCTGAAACCCGGCTGGTTCAACGGGAA
ACTGGTGCATGATTATCCCCCAACCGATTCTAAAACCGGGCGATTGAATA
ACACTAATCAATCACCGATTATCAGAAATCGATACAGCCCAATCCAAGAG
AAACACTGGGAAGTTCTCATAATGACACTGTGCCATTTCCATCGGCAGCA
CGATGTGAATTCCACCTCAGCTTATCAAGTCATCCAATGGATTTGCCGAC
AATGTCTCAGCCCGTCGTATTATCTCCTCATCAATGCAGATCTCAATACG
ATACCGAAGGATCTCAACTATATTACTATCTTCTGGAAACGATTCAACCA
ATCGGAATTGTTCTGGGGTTTATTCTATATGCTCTCTCGCAAGGCGCCTC
AAACATTTCAGCCTCCCTATCAAACTCTGGAAGCATTAATGTCAGCTTGT
CAACATCTCTGGTCCACGTCTGATTGTCCCAGAAAACGGCTGATTCGCAA
CGTGCCTGACAGTGAAATATTTATCAGTGATAGCCATGATCCAATTATCC
TCCATAAACAACGATTCTACGAATTAATTAACTCAAATTCTAATCTCTAC
CGAGCAACAGTGTTCCTGTTTGAATGCCTCTCGTCGCAAATGGCTCTGAC
AGATGAATTGAAATTGATGCTGCCATATCTCAGTTGTTCTGAGGTGCAAA
CCATTCTATTTCACTTGGCATACATTTGGAACAAGAAGGTCCAGAAGCCG
CCAACAGTGCCAGCACCGGTGAATGAAATCGATGCCGATGGGAATGTTGT
ATCGGTTTCCATGGTGCCTATAGAAGATACCCAACCGAAAAACACATTTT
CATGGGAAACTCTGCTGCCGGCACTTGTGCACGCCAGAATTCATTTCAAA
TCACTCTATGATCAATTTTGTCGATTGTTATTTATATCCTGCGAATAAAA
TTTTGTCACTAATAAAATTCTTGTTTGTATATTTTTCATTGATTGATTTG
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protein sequence of SMED30023417-orf-1

>SMED30023417-orf-1 ID=SMED30023417-orf-1|Name=SMED30023417-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=126bp
MIWTAEIVCLGSVVRRKFTRKSVRRRIRMTEEGLLAFRDSIKIDSITEMH
RTTREATKIGQALNLVSVLHRYGDDIGTEYFSSLTTSILERIEMEILMIG
KDLLVTTSNSSKSILEFIALANRRR*
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protein sequence of SMED30023417-orf-2

>SMED30023417-orf-2 ID=SMED30023417-orf-2|Name=SMED30023417-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=981bp
MTSVELSWKKLVNIVSLQHDIVKHKDTISEAFHLFPPSRPALLDIFCDII
VDKYVGSYIHQRNNYTNELEEIITSLTNEVNIILDKEFESNDYVLTAVNT
KVSHYSGYGFVVYACQWVLQVLAKITMKYDQILAFNEPLSVNDKLKWTQV
PVIINLLDILCKTLKSKMVGHETVSIIMKNVAQHRNHLLWVIKFIGERHP
SGIVDKIIDCILSEFIPTQEYEKLQSCMVVLSSLGAHHARQLQSALYKKL
KAAIEGSFETVPKQKIINYQTLIYFTLLLASRSDVFELTNASISMIGRII
CQFTPKQLEQFFLHQNFGDNILRSYYHMTLNILLNSHDLEIGRVLEFIIH
HTHSTITGRTGVSCCSANFSQMCMNIIANVSVKLLIQCHSAGLSKPSNNR
TMALAILNYVMQHLEFWIQRVVKDGSCVAAKLLSCLVLNDFKSIGDDLLL
TIVTNDPQTPTEVNGHKKALVLFFRLVDEFESVSYNILEEVTTRSLCVAQ
SYQPSRQMRYLSTLYSMLVYEARMQSQRRRLARAINSNIDLLLLLVVTNK
SLPIIKISISILSKIDVVSDEKYSVPISSPYLCKTDTRFKAWPIFVASRV
VRCISVMLSILIESLNANNPSSVILIRRLTDFLVNFLRTTDPRHTISAVQ
IIFREFLRSSWIDTNNRFVFKKTLKPGWFNGKLVHDYPPTDSKTGRLNNT
NQSPIIRNRYSPIQEKHWEVLIMTLCHFHRQHDVNSTSAYQVIQWICRQC
LSPSYYLLINADLNTIPKDLNYITIFWKRFNQSELFWGLFYMLSRKAPQT
FQPPYQTLEALMSACQHLWSTSDCPRKRLIRNVPDSEIFISDSHDPIILH
KQRFYELINSNSNLYRATVFLFECLSSQMALTDELKLMLPYLSCSEVQTI
LFHLAYIWNKKVQKPPTVPAPVNEIDADGNVVSVSMVPIEDTQPKNTFSW
ETLLPALVHARIHFKSLYDQFCRLLFISCE*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0002109X1 cell
PLANA:0002111X2 cell
Vocabulary: INTERPRO
TermDefinition
IPR029445INTS5_N
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR029445Integrator complex subunit 5, N-terminalPFAMPF14837INTS5_Ncoord: 21..215
e-value: 6.3E-11
score: 42.3