Sn1-specific diacylglycerol lipase beta

Overview
NameSn1-specific diacylglycerol lipase beta
Smed IDSMED30023344
Length (bp)2484
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Sn1-specific diacylglycerol lipase beta (SMED30023344) t-SNE clustered cells

Violin plots show distribution of expression levels for Sn1-specific diacylglycerol lipase beta (SMED30023344) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Sn1-specific diacylglycerol lipase beta (SMED30023344) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Sn1-specific diacylglycerol lipase beta (SMED30023344) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30023344

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 12

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30023344h1SMcG0021548 SmedASXL_037154SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016
whole organism asexual adult RNA-sequencing evidence
head regionSMED30023344h1SMcG0021548 SmedASXL_014928SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016
whole organism asexual adult RNA-sequencing evidence
nervous systemSMED30023344h1SMcG0021548 dd_Smed_v4_13306_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30023344h1SMcG0021548 dd_Smed_v4_13306_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neuronSMED30023344h1SMcG0021548 dd_Smed_v4_13306_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult colorimetric in situ hybridization evidence
muscle cellSMED30023344h1SMcG0021548 dd_Smed_v4_13306_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30023344h1SMcG0021548 dd_Smed_v4_13306_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30023344h1SMcG0021548 dd_Smed_v4_13306_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30023344h1SMcG0021548 dd_Smed_v6_13306_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017
whole organism asexual adult RNA-sequencing evidence
pigment cellSMED30023344h1SMcG0021548 SMED30023344smed_20140614PMID:22884275
Lapan et al., 2012
whole organism asexual adult colorimetric in situ hybridization evidence
reproductive organSMED30023344h1SMcG0021548 Contig41234uc_Smed_v2PMID:28434803
Rouhana et al., 2017
whole organism adult hermaphrodite RNA-sequencing evidence
reproductive organSMED30023344h1SMcG0021548 Contig41234newmark_estsPMID:28434803
Rouhana et al., 2017
whole organism adult hermaphrodite RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of Sn1-specific diacylglycerol lipase beta vs. Ensembl Human
Match: DAGLA (diacylglycerol lipase alpha [Source:HGNC Symbol;Acc:HGNC:1165])

HSP 1 Score: 202.986 bits (515), Expect = 1.876e-53
Identity = 180/766 (23.50%), Postives = 323/766 (42.17%), Query Frame = 2
Query:  104 MPPLIMFGRKWNIATDDFVYLSFWEFLIRL--FIIVAYVYFNFHENMINNCNSKLYQICHFLLSGSLTCNXXXXXXXXXXXXQGLIMEESKRSKIPFCIYIKLFMSFYEAILSIVAFAINYRY-----SLIPNKFCEQILIPNKIILYCIGPFLTAYLIIFCITYDQLGRHGSNVILLATKK------------------TNIWKHRLKWILCIKKVNDEERNALDSAANIFASLCEESNLVFSDFEAGIQLLCKQDANAYDFTEYSLKINKSKSGVTNSSSFNQKMYTIINHFINNEENYVNMSNFKKLANENLIETDSKVNNSEVNLADKISTGNKSDLKSDWRDIRF--IYYFSTMAKASYGWPIHLLFHDENKSRLKFCMGLCCKHSTSCFCNKCICCDCEKSNSNDQKLIYVKERFCSCHEKAILNETHLEKENLVCV-----SFYNKTFHSPFFIAISHEIEVIILSIRGTFSLKDILTDMDANLIEVFEDGFSG-YLAHSGMLNAAKNIKRKIQEENII-----HNILNDNKNYRLIITGHSLGAGIAIILSCFYYHEFPNLKCYAFSPPLGTVNIKTADYCKDFVLSIVNGVEWVPRISLQSIVVAKRRLIECLENYPHXXXXXXXXXXXXXXXXXXXXXXXRWNILNSFER---DEKYWENIEI----NTKL-------------------PGQIIHITNIKKLNDP-NKSDLINSNRSFYTPIITSSDQFKTVVFHPKMFKDHFPHQMVKRLFNIL 2206
            MP +++F R+W++ +DD V  + + FL+    F+I++ V F    N    C+  L       L   L+C +  +  I +    G++  E  R  + + +Y++L +   E I +IV      +Y      L        +++ N +++      L+  + + C+ +D  GR  + V L ATK+                     W  RLK  LC  +  D + +A    A +FA    + ++V SD  AG+ LL ++               ++K       + N  +  +    +     Y+++ N +++                                     +R+  + Y+   A A+YGWP++L+       R   C GLC           C CC C         +   ++  C C+  AI    H   EN+  V     S ++  + +PF++A+ H+ + +++SIRGT S KD LTD+  +   +  +G  G +L H GM+ +A+ IK+K+++E ++      ++    K+Y LI+ GHSLGAG A ILS     ++P LKC+A+SPP G ++    +Y K+FV ++V G + VPRI L  +   +R+L++ L+                        T  +W I+    +     +  E +E+    +T+L                   PG+IIH+ +    N P  +          Y  I   +  F  V+  P M  +H P+ +++ L  +L
Sbjct:    1 MPGIVVFRRRWSVGSDDLVLPAIFLFLLHTTWFVILSVVLFGLVYNPHEACSLNLVDHGRGYLGILLSCMIAEMAIIWLSMRGGILYTE-PRDSMQYVLYVRLAILVIEFIYAIVGIVWLTQYYTSCNDLTAKNVTLGMVVCNWVVI------LSVCITVLCV-FDPTGR--TFVKLRATKRRQRNLRTYNLRHRLEEGQATSWSRRLKVFLCCTRTKDSQSDAYSEIAYLFAEFFRDLDIVPSDIIAGLVLLRQR--------------QRAKRNAVLDEANNDILAFLSGMPVTRNTKYLDLKNSQEM-------------------------------------LRYKEVCYYMLFALAAYGWPMYLM-------RKPAC-GLCQ------LARSCSCCLCPARPRFAPGVTIEEDNCCGCNAIAI--RRHFLDENMTAVDIVYTSCHDAVYETPFYVAVDHDKKKVVISIRGTLSPKDALTDLTGDAERLPVEGHHGTWLGHKGMVLSAEYIKKKLEQEMVLSQAFGRDLGRGTKHYGLIVVGHSLGAGTAAILSFLLRPQYPTLKCFAYSPPGGLLSEDAMEYSKEFVTAVVLGKDLVPRIGLSQLEGFRRQLLDVLQR----------------------STKPKWRIIVGATKCIPKSELPEEVEVTTLASTRLWTHPSDLTIALSASTPLYPPGRIIHVVH----NHPAEQCCCCEQEEPTYFAIWGDNKAFNEVIISPAMLHEHLPYVVMEGLNKVL 663          
BLAST of Sn1-specific diacylglycerol lipase beta vs. Ensembl Human
Match: DAGLB (diacylglycerol lipase beta [Source:HGNC Symbol;Acc:HGNC:28923])

HSP 1 Score: 191.045 bits (484), Expect = 1.078e-50
Identity = 151/611 (24.71%), Postives = 282/611 (46.15%), Query Frame = 2
Query:  104 MPPLIMFGRKWNIATDDFVYLSFWEFLIR-LFIIVAYVYFNFHENMINNCNSKL---YQICHFLLSGSLTCNXXXXXXXXXXXXQGLIMEESKRSKIPFCIYIKLFMSFYEAILSIVAFAINYRYSLIPNKFCEQILIPNKIILYCIGPFL--TAYLIIFCITYDQLG---------------RHGSNVIL--LATKKTNIWKHRLKWILCIKKVNDEERNALDSAANIFASLCEESNLVFSDFEAGIQLLCKQDANAYDFTEYSLKINKSKSGVTNSSSFNQKMYTIINHFINNEENYVNMSNFKKLANENLIETDSKVNNSEVNLADKISTGNKSDLKSDWRDIRFIYYFSTMAKASYGWPIHLLFHDENKSRLKFCMGLCCKHSTSCFCNKCICCDCEKSNSNDQKLIYVKERFCSCHEKAILNETHLEKENLVCVSFYNKTFHSPFFIAISHEIEVIILSIRGTFSLKDILTDMDAN--LIEVFEDGFSGYLAHSGMLNAAKNIKRKIQEENIIHNILNDNKNYRLIITGHSLGAGIAIILSCFYYHEFPNLKCYAFSPPLGTVNIKTADYCKDFVLSIVNGVEWVPRISLQSIVVAKRRLIECLEN 1861
            MP +++FGR+W IA+DD V+  F+E ++R L+ I     +  H   ++     L   Y I   +L   + C +  I+ + +   +G I     R  +   +YI+L + F E + + +  A      +     C++ ++ N II   +  ++   A ++   I +D LG                H S+ +L  L T  T++W+ R+K + C    +D  R A  S A +F++   +++LV SD  AG+ LL +Q  N  +                     NQ+   ++ H   + +                 + D+++ N                           +++   A A+YGWP+++                  ++  +  C   I  DC +S + D  L+   +   +CH  +IL+ T L+  + + VSF++K +  PF +A+ H  E +++++RGT SL+D+LTD+ A   +++V E      LAH G+  AA+ + +++  + I+    +    YRL+I GHSLG G A +L+      +P ++CYAFSPP G  +    +Y + F++S+V G + +PR+S+ ++   KRR++  + +
Sbjct:    1 MPGMVLFGRRWAIASDDLVFPGFFELVVRVLWWIGILTLYLMHRGKLDCAGGALLSSYLIVLMILLAVVICTVSAIMCVSM---RGTICNPGPRKSMSKLLYIRLALFFPEMVWASLGAAW-----VADGVQCDRTVV-NGIIATVVVSWIIIAATVVSIIIVFDPLGGKMAPYSSAGPSHLDSHDSSQLLNGLKTAATSVWETRIKLLCCCIGKDDHTRVAFSSTAELFSTYFSDTDLVPSDIAAGLALLHQQQDNIRN---------------------NQEPAQVVCHAPGSSQE---------------ADLDAELENC--------------------------HHYMQFAAAAYGWPLYIY-----------------RNPLTGLCR--IGGDCCRSRTTDYDLVGGDQ--LNCHFGSILHTTGLQYRDFIHVSFHDKVYELPFLVALDHRKESVVVAVRGTMSLQDVLTDLSAESEVLDV-ECEVQDRLAHKGISQAARYVYQRLINDGILSQAFSIAPEYRLVIVGHSLGGGAAALLATMLRAAYPQVRCYAFSPPRGLWSKALQEYSQSFIVSLVLGKDVIPRLSVTNLEDLKRRILRVVAH 518          
BLAST of Sn1-specific diacylglycerol lipase beta vs. Ensembl Human
Match: DAGLB (diacylglycerol lipase beta [Source:HGNC Symbol;Acc:HGNC:28923])

HSP 1 Score: 156.377 bits (394), Expect = 3.202e-39
Identity = 127/527 (24.10%), Postives = 241/527 (45.73%), Query Frame = 2
Query:  344 QGLIMEESKRSKIPFCIYIKLFMSFYEAILSIVAFAINYRYSLIPNKFCEQILIPNKIILYCIGPFL--TAYLIIFCITYDQLG---------------RHGSNVIL--LATKKTNIWKHRLKWILCIKKVNDEERNALDSAANIFASLCEESNLVFSDFEAGIQLLCKQDANAYDFTEYSLKINKSKSGVTNSSSFNQKMYTIINHFINNEENYVNMSNFKKLANENLIETDSKVNNSEVNLADKISTGNKSDLKSDWRDIRFIYYFSTMAKASYGWPIHLLFHDENKSRLKFCMGLCCKHSTSCFCNKCICCDCEKSNSNDQKLIYVKERFCSCHEKAILNETHLEKENLVCVSFYNKTFHSPFFIAISHEIEVIILSIRGTFSLKDILTDMDAN--LIEVFEDGFSGYLAHSGMLNAAKNIKRKIQEENIIHNILNDNKNYRLIITGHSLGAGIAIILSCFYYHEFPNLKCYAFSPPLGTVNIKTADYCKDFVLSIVNGVEWVPRISLQSIVVAKRRLIECLEN 1861
            +G I     R  +   +YI+L + F E + + +  A      +     C++ ++ N II   +  ++   A ++   I +D LG                H S+ +L  L T  T++W+ R+K + C    +D  R A  S A +F++   +++LV SD  AG+ LL                 ++ +  + N    NQ+   ++ H   + +                 + D+++ N                           +++   A A+YGWP+++                  ++  +  C   I  DC +S + D  L+   +   +CH  +IL+ T L+  + + VSF++K +  PF +A+ H  E +++++RGT SL+D+LTD+ A   +++V E      LAH G+  AA+ + +++  + I+    +    YRL+I GHSLG G A +L+      +P ++CYAFSPP G  +    +Y + F++S+V G + +PR+S+ ++   KRR++  + +
Sbjct:   41 RGTICNPGPRKSMSKLLYIRLALFFPEMVWASLGAAW-----VADGVQCDRTVV-NGIIATVVVSWIIIAATVVSIIIVFDPLGGKMAPYSSAGPSHLDSHDSSQLLNGLKTAATSVWETRIKLLCCCIGKDDHTRVAFSSTAELFSTYFSDTDLVPSDIAAGLALL-----------------HQQQDNIRN----NQEPAQVVCHAPGSSQE---------------ADLDAELENC--------------------------HHYMQFAAAAYGWPLYIY-----------------RNPLTGLCR--IGGDCCRSRTTDYDLVGGDQ--LNCHFGSILHTTGLQYRDFIHVSFHDKVYELPFLVALDHRKESVVVAVRGTMSLQDVLTDLSAESEVLDV-ECEVQDRLAHKGISQAARYVYQRLINDGILSQAFSIAPEYRLVIVGHSLGGGAAALLATMLRAAYPQVRCYAFSPPRGLWSKALQEYSQSFIVSLVLGKDVIPRLSVTNLEDLKRRILRVVAH 477          
BLAST of Sn1-specific diacylglycerol lipase beta vs. Ensembl Human
Match: DAGLB (diacylglycerol lipase beta [Source:HGNC Symbol;Acc:HGNC:28923])

HSP 1 Score: 125.176 bits (313), Expect = 2.826e-29
Identity = 69/210 (32.86%), Postives = 126/210 (60.00%), Query Frame = 2
Query: 1238 KSNSNDQKLIYVKERFCSCHEKAILNETHLEKENLVCVSFYNKTFHSPFFIAISHEIEVIILSIRGTFSLKDILTDMDAN--LIEVFEDGFSGYLAHSGMLNAAKNIKRKIQEENIIHNILNDNKNYRLIITGHSLGAGIAIILSCFYYHEFPNLKCYAFSPPLGTVNIKTADYCKDFVLSIVNGVEWVPRISLQSIVVAKRRLIECLEN 1861
            +S + D  L+   +   +CH  +IL+ T L+  + + VSF++K +  PF +A+ H  E +++++RGT SL+D+LTD+ A   +++V E      LAH G+  AA+ + +++  + I+    +    YRL+I GHSLG G A +L+      +P ++CYAFSPP G  +    +Y + F++S+V G + +PR+S+ ++   KRR++  + +
Sbjct:  183 RSRTTDYDLVGGDQ--LNCHFGSILHTTGLQYRDFIHVSFHDKVYELPFLVALDHRKESVVVAVRGTMSLQDVLTDLSAESEVLDV-ECEVQDRLAHKGISQAARYVYQRLINDGILSQAFSIAPEYRLVIVGHSLGGGAAALLATMLRAAYPQVRCYAFSPPRGLWSKALQEYSQSFIVSLVLGKDVIPRLSVTNLEDLKRRILRVVAH 389          
BLAST of Sn1-specific diacylglycerol lipase beta vs. Ensembl Celegans
Match: dagl-2 (DiAcylGlycerol Lipase homolog [Source:UniProtKB/TrEMBL;Acc:Q65ZK3])

HSP 1 Score: 130.183 bits (326), Expect = 6.238e-31
Identity = 81/290 (27.93%), Postives = 146/290 (50.34%), Query Frame = 2
Query: 1061 WRDIRFIYYFSTMAKASYGWPIHLLFHDENKSRLKFCMGLCCKHSTSCFCNKCICCDCEKSN------SNDQ-----KLIYVKERFCSCHEKAILNETHLEKENLVCVSFYNKTFHSPFFIAISHEIEVIILSIRGTFSLKDILTDM-------------DANLIEVFE-DGFSGYLAHSGMLNAAKNIKRKIQEENIIHNILNDNKNYRLIITGHSLGAGIAIILSCFYYHEFPNLKCYAFSPPLGTVNIKTADYCKDFVLSIVNGVEWVPRISLQSIVVAKRRLIECL 1855
            W +++   YF   A   YGWP ++L++   KS  +    L C     C  + C+     +        SN +     K + V++  C C+  A++        +L  +SF N+ +  PF +   H+ + I+++IRG+ SL D++TD+             DA L E  E D       H GML +A+ +   + +  I++++   N +Y+L++ GHSLGAG+  +L+     E+P++ CYAF+PP   ++    D  + +V+S+V+G + V R+S QS+   + R+ + L
Sbjct:  242 WMNLKDAEYFLHHATCVYGWPTYILYNCGLKSIYRLFRKLQCCGRMRCAQSPCVVQQQRRERIELTELSNGKLVKKGKSMVVEDNCCYCNTAAVVLANEARNIDLQFMSFRNRVYEVPFAVIADHDKKSIVITIRGSCSLIDLVTDLSLEDELMTVDVDQDATLREDEEIDKRGDVRVHRGMLRSARYVFDTLNKNKILNDLFISNPSYQLVVCGHSLGAGVGSLLTMLLKQEYPSVICYAFAPPGCVISEFGQDEMEKYVMSVVSGDDIVSRMSFQSLHRLRERVFQEL 531          

HSP 2 Score: 64.3142 bits (155), Expect = 2.662e-10
Identity = 70/242 (28.93%), Postives = 115/242 (47.52%), Query Frame = 2
Query:  104 MPPLIMFGRKWNIATDDFVYLSFWEFLIRL-FIIVAYVYFNFHENMINNCNSKLYQICHFLLSGSLTCNXXXXXXXXXXXXQGLIMEESKRSKIPFCIYIKLFMSFYEAILSIVAFAINYRYSLIPNKFCEQILIPNKIIL---YCIGPFLTAYLIIFCITYDQLGRHGSNVILLATKKTNIWKHRLKWILCIKKVNDEE--RNALDSAANIFASLCEESNLVFSDFEAGIQLLCKQDANAY 811
            MP L+ F R+WNI +DDFV+    E L+RL ++      F FH  +  + ++   Q+   L+   +T   ++ +   ++SA+G IM    R  IP  +YI+L +   E  L+I+A  I+   S    + C+   I    I+   + I       +I+F +T ++L    S    +A K    W  R++    I K+  +   R ALD  A + +S   +S+LV SD  AG+ L      N Y
Sbjct:    1 MPSLVAFNRQWNIGSDDFVFPEITEALVRLSWVAFGVSVFVFHFPLACSTHNLTLQLLGLLVINVITI--LLAILTAVISARGTIMNSQPRKLIPRLLYIRLPIFIAEIALTILA-TIHAFKSTNGGEGCDFATIARVTIILEWFLIFTAFAGLVIVFHLTENELEDSAS----IAQKS---WSRRMR----IFKIGQDHSMRVALDDLAILISSFFVDSDLVPSDVVAGLLLAYHSPNNQY 228          
BLAST of Sn1-specific diacylglycerol lipase beta vs. Ensembl Celegans
Match: dagl-2 (DiAcylGlycerol Lipase homolog [Source:UniProtKB/TrEMBL;Acc:Q65ZK3])

HSP 1 Score: 128.257 bits (321), Expect = 2.347e-30
Identity = 79/279 (28.32%), Postives = 141/279 (50.54%), Query Frame = 2
Query: 1061 WRDIRFIYYFSTMAKASYGWPIHLLFHDENKSRLKFCMGLCCKHSTSCFCNKCICCDCEKSNSNDQKLIYVKERFCSCHEKAILNETHLEKENLVCVSFYNKTFHSPFFIAISHEIEVIILSIRGTFSLKDILTDM-------------DANLIEVFE-DGFSGYLAHSGMLNAAKNIKRKIQEENIIHNILNDNKNYRLIITGHSLGAGIAIILSCFYYHEFPNLKCYAFSPPLGTVNIKTADYCKDFVLSIVNGVEWVPRISLQSIVVAKRRLIECL 1855
            W +++   YF   A   YGWP ++L++   KS  +    L C       C +  C           K + V++  C C+  A++        +L  +SF N+ +  PF +   H+ + I+++IRG+ SL D++TD+             DA L E  E D       H GML +A+ +   + +  I++++   N +Y+L++ GHSLGAG+  +L+     E+P++ CYAF+PP   ++    D  + +V+S+V+G + V R+S QS+   + R+ + L
Sbjct:  244 WMNLKDAEYFLHHATCVYGWPTYILYNCGLKSIYRLFRKLQC-------CGRMRCAQ--------GKSMVVEDNCCYCNTAAVVLANEARNIDLQFMSFRNRVYEVPFAVIADHDKKSIVITIRGSCSLIDLVTDLSLEDELMTVDVDQDATLREDEEIDKRGDVRVHRGMLRSARYVFDTLNKNKILNDLFISNPSYQLVVCGHSLGAGVGSLLTMLLKQEYPSVICYAFAPPGCVISEFGQDEMEKYVMSVVSGDDIVSRMSFQSLHRLRERVFQEL 507          

HSP 2 Score: 61.6178 bits (148), Expect = 2.009e-9
Identity = 69/243 (28.40%), Postives = 114/243 (46.91%), Query Frame = 2
Query:  104 MPPLIMFGRKWNIATDDFVYLSFWEFLIRL-FIIVAYVYFNFHENMINNCNSKLYQICHFLLSGSLTCNXXXXXXXXXXXXQGLIMEESKRSKIPFCIYIKLFMSFYEAILSIVAFAINYRY-SLIPNKFCEQILIPNKIIL---YCIGPFLTAYLIIFCITYDQLGRHGSNVILLATKKTNIWKHRLKWILCIKKVNDEE--RNALDSAANIFASLCEESNLVFSDFEAGIQLLCKQDANAY 811
            MP L+ F R+WNI +DDFV+    E L+RL ++      F FH  +  + ++   Q+   L+   +T   ++ +   ++SA+G IM    R  IP  +YI+L +   E  L+I+A    +   S    + C+   I    I+   + I       +I+F +T ++L    S    +A K    W  R++    I K+  +   R ALD  A + +S   +S+LV SD  AG+ L      N Y
Sbjct:    1 MPSLVAFNRQWNIGSDDFVFPEITEALVRLSWVAFGVSVFVFHFPLACSTHNLTLQLLGLLVINVITI--LLAILTAVISARGTIMNSQPRKLIPRLLYIRLPIFIAEIALTILATIHAFILESTNGGEGCDFATIARVTIILEWFLIFTAFAGLVIVFHLTENELEDSAS----IAQKS---WSRRMR----IFKIGQDHSMRVALDDLAILISSFFVDSDLVPSDVVAGLLLAYHSPNNQY 230          
BLAST of Sn1-specific diacylglycerol lipase beta vs. Ensembl Celegans
Match: dagl-2 (DiAcylGlycerol Lipase homolog [Source:UniProtKB/TrEMBL;Acc:Q65ZK3])

HSP 1 Score: 128.257 bits (321), Expect = 2.364e-30
Identity = 79/279 (28.32%), Postives = 141/279 (50.54%), Query Frame = 2
Query: 1061 WRDIRFIYYFSTMAKASYGWPIHLLFHDENKSRLKFCMGLCCKHSTSCFCNKCICCDCEKSNSNDQKLIYVKERFCSCHEKAILNETHLEKENLVCVSFYNKTFHSPFFIAISHEIEVIILSIRGTFSLKDILTDM-------------DANLIEVFE-DGFSGYLAHSGMLNAAKNIKRKIQEENIIHNILNDNKNYRLIITGHSLGAGIAIILSCFYYHEFPNLKCYAFSPPLGTVNIKTADYCKDFVLSIVNGVEWVPRISLQSIVVAKRRLIECL 1855
            W +++   YF   A   YGWP ++L++   KS  +    L C       C +  C           K + V++  C C+  A++        +L  +SF N+ +  PF +   H+ + I+++IRG+ SL D++TD+             DA L E  E D       H GML +A+ +   + +  I++++   N +Y+L++ GHSLGAG+  +L+     E+P++ CYAF+PP   ++    D  + +V+S+V+G + V R+S QS+   + R+ + L
Sbjct:  242 WMNLKDAEYFLHHATCVYGWPTYILYNCGLKSIYRLFRKLQC-------CGRMRCAQ--------GKSMVVEDNCCYCNTAAVVLANEARNIDLQFMSFRNRVYEVPFAVIADHDKKSIVITIRGSCSLIDLVTDLSLEDELMTVDVDQDATLREDEEIDKRGDVRVHRGMLRSARYVFDTLNKNKILNDLFISNPSYQLVVCGHSLGAGVGSLLTMLLKQEYPSVICYAFAPPGCVISEFGQDEMEKYVMSVVSGDDIVSRMSFQSLHRLRERVFQEL 505          

HSP 2 Score: 64.3142 bits (155), Expect = 2.489e-10
Identity = 70/242 (28.93%), Postives = 115/242 (47.52%), Query Frame = 2
Query:  104 MPPLIMFGRKWNIATDDFVYLSFWEFLIRL-FIIVAYVYFNFHENMINNCNSKLYQICHFLLSGSLTCNXXXXXXXXXXXXQGLIMEESKRSKIPFCIYIKLFMSFYEAILSIVAFAINYRYSLIPNKFCEQILIPNKIIL---YCIGPFLTAYLIIFCITYDQLGRHGSNVILLATKKTNIWKHRLKWILCIKKVNDEE--RNALDSAANIFASLCEESNLVFSDFEAGIQLLCKQDANAY 811
            MP L+ F R+WNI +DDFV+    E L+RL ++      F FH  +  + ++   Q+   L+   +T   ++ +   ++SA+G IM    R  IP  +YI+L +   E  L+I+A  I+   S    + C+   I    I+   + I       +I+F +T ++L    S    +A K    W  R++    I K+  +   R ALD  A + +S   +S+LV SD  AG+ L      N Y
Sbjct:    1 MPSLVAFNRQWNIGSDDFVFPEITEALVRLSWVAFGVSVFVFHFPLACSTHNLTLQLLGLLVINVITI--LLAILTAVISARGTIMNSQPRKLIPRLLYIRLPIFIAEIALTILA-TIHAFKSTNGGEGCDFATIARVTIILEWFLIFTAFAGLVIVFHLTENELEDSAS----IAQKS---WSRRMR----IFKIGQDHSMRVALDDLAILISSFFVDSDLVPSDVVAGLLLAYHSPNNQY 228          
BLAST of Sn1-specific diacylglycerol lipase beta vs. Ensembl Fly
Match: inaE (gene:FBgn0261244 transcript:FBtr0073826)

HSP 1 Score: 157.147 bits (396), Expect = 1.122e-39
Identity = 110/421 (26.13%), Postives = 193/421 (45.84%), Query Frame = 2
Query:  638 WKHRLKWILCIKKVNDEERNALDSAANIFASLCEESNLVFSDFEAGIQLLCKQDANAYDFTEYSLKINKSKSGVTNSSSFNQKMYTIINHF-INNEENYVNMSNFKKLANENLIETDSKVNNSEVNLADKISTGNKSDLKSDWRDIRFIYYFSTMAKASYGWPIHLLFHDENKSRLKF------CMGLCCKHSTSCFCNKCICCDCEKSNSNDQKLIYVKERFCSCHEKAILNETHLEKENLVCVSFYNKTFHSPFFIAISHEIEVIILSIRGTFSLKDILTDMDA-NLIEVFEDGFSGYLAHSGMLNAAKNIKRKIQEENIIHNILNDNKN-----YRLIITGHSLGAGIAIILSCFYYHEFPNLKCYAFSPPLGTVNIKTADYCKDFVLSIVNGVEWVPRISLQSIVVAKRRLIECLEN 1861
            W HR + + C    ++  RN+    A + +    E ++V SD  AG+ LL K     +   E    + + K+G           Y  ++   I     ++ +++ K   N +  +T                             I ++Y+    A+ +YGWP++++    N+S++        C G CC                    S+D ++I  ++  C C+  A+     L   ++V  +++     +PFF+AI +    +++SIRGT S+KDILTD++A   +   +     +L H GM+ AA  I+ K+QEEN+I   L  N +     + L++ GHSLGAG A IL+     E P L+C+++SPP G +++   +Y K F+ S+V G + VPRI L  +   +  LI  ++ 
Sbjct:  206 WDHRCRLLFCCMGSSERNRNSFTDIARLLSDFFRELDVVPSDVVAGLVLLRK-----FQRLEREAIVRQRKNGT----------YEFLSGVPITERTQFLALNDAK---NYDFFQTV----------------------------IHYMYF----AQGAYGWPMYVII---NRSKMWHLVPELKCFGCCC------------------GTSDDTEVI--QDNCCLCNYAALKKTLQLGDIDIVYATYHVDVGETPFFVAIDYTHRAVVISIRGTLSMKDILTDLNAEGEVLPLQPPRDDWLGHKGMVQAAIYIRNKLQEENLIERALQRNPDRQTHTFDLVLVGHSLGAGTAAILAILLKPEHPTLQCFSYSPPGGLLSMPAVEYSKSFITSVVLGKDVVPRIGLNQMEALRADLINAIQR 553          
BLAST of Sn1-specific diacylglycerol lipase beta vs. Ensembl Fly
Match: inaE (gene:FBgn0261244 transcript:FBtr0073825)

HSP 1 Score: 157.532 bits (397), Expect = 1.422e-39
Identity = 110/421 (26.13%), Postives = 193/421 (45.84%), Query Frame = 2
Query:  638 WKHRLKWILCIKKVNDEERNALDSAANIFASLCEESNLVFSDFEAGIQLLCKQDANAYDFTEYSLKINKSKSGVTNSSSFNQKMYTIINHF-INNEENYVNMSNFKKLANENLIETDSKVNNSEVNLADKISTGNKSDLKSDWRDIRFIYYFSTMAKASYGWPIHLLFHDENKSRLKF------CMGLCCKHSTSCFCNKCICCDCEKSNSNDQKLIYVKERFCSCHEKAILNETHLEKENLVCVSFYNKTFHSPFFIAISHEIEVIILSIRGTFSLKDILTDMDA-NLIEVFEDGFSGYLAHSGMLNAAKNIKRKIQEENIIHNILNDNKN-----YRLIITGHSLGAGIAIILSCFYYHEFPNLKCYAFSPPLGTVNIKTADYCKDFVLSIVNGVEWVPRISLQSIVVAKRRLIECLEN 1861
            W HR + + C    ++  RN+    A + +    E ++V SD  AG+ LL K     +   E    + + K+G           Y  ++   I     ++ +++ K   N +  +T                             I ++Y+    A+ +YGWP++++    N+S++        C G CC                    S+D ++I  ++  C C+  A+     L   ++V  +++     +PFF+AI +    +++SIRGT S+KDILTD++A   +   +     +L H GM+ AA  I+ K+QEEN+I   L  N +     + L++ GHSLGAG A IL+     E P L+C+++SPP G +++   +Y K F+ S+V G + VPRI L  +   +  LI  ++ 
Sbjct:  206 WDHRCRLLFCCMGSSERNRNSFTDIARLLSDFFRELDVVPSDVVAGLVLLRK-----FQRLEREAIVRQRKNGT----------YEFLSGVPITERTQFLALNDAK---NYDFFQTV----------------------------IHYMYF----AQGAYGWPMYVII---NRSKMWHLVPELKCFGCCC------------------GTSDDTEVI--QDNCCLCNYAALKKTLQLGDIDIVYATYHVDVGETPFFVAIDYTHRAVVISIRGTLSMKDILTDLNAEGEVLPLQPPRDDWLGHKGMVQAAIYIRNKLQEENLIERALQRNPDRQTHTFDLVLVGHSLGAGTAAILAILLKPEHPTLQCFSYSPPGGLLSMPAVEYSKSFITSVVLGKDVVPRIGLNQMEALRADLINAIQR 553          
BLAST of Sn1-specific diacylglycerol lipase beta vs. Ensembl Fly
Match: inaE (gene:FBgn0261244 transcript:FBtr0290303)

HSP 1 Score: 157.918 bits (398), Expect = 1.495e-39
Identity = 110/421 (26.13%), Postives = 193/421 (45.84%), Query Frame = 2
Query:  638 WKHRLKWILCIKKVNDEERNALDSAANIFASLCEESNLVFSDFEAGIQLLCKQDANAYDFTEYSLKINKSKSGVTNSSSFNQKMYTIINHF-INNEENYVNMSNFKKLANENLIETDSKVNNSEVNLADKISTGNKSDLKSDWRDIRFIYYFSTMAKASYGWPIHLLFHDENKSRLKF------CMGLCCKHSTSCFCNKCICCDCEKSNSNDQKLIYVKERFCSCHEKAILNETHLEKENLVCVSFYNKTFHSPFFIAISHEIEVIILSIRGTFSLKDILTDMDA-NLIEVFEDGFSGYLAHSGMLNAAKNIKRKIQEENIIHNILNDNKN-----YRLIITGHSLGAGIAIILSCFYYHEFPNLKCYAFSPPLGTVNIKTADYCKDFVLSIVNGVEWVPRISLQSIVVAKRRLIECLEN 1861
            W HR + + C    ++  RN+    A + +    E ++V SD  AG+ LL K     +   E    + + K+G           Y  ++   I     ++ +++ K   N +  +T                             I ++Y+    A+ +YGWP++++    N+S++        C G CC                    S+D ++I  ++  C C+  A+     L   ++V  +++     +PFF+AI +    +++SIRGT S+KDILTD++A   +   +     +L H GM+ AA  I+ K+QEEN+I   L  N +     + L++ GHSLGAG A IL+     E P L+C+++SPP G +++   +Y K F+ S+V G + VPRI L  +   +  LI  ++ 
Sbjct:  206 WDHRCRLLFCCMGSSERNRNSFTDIARLLSDFFRELDVVPSDVVAGLVLLRK-----FQRLEREAIVRQRKNGT----------YEFLSGVPITERTQFLALNDAK---NYDFFQTV----------------------------IHYMYF----AQGAYGWPMYVII---NRSKMWHLVPELKCFGCCC------------------GTSDDTEVI--QDNCCLCNYAALKKTLQLGDIDIVYATYHVDVGETPFFVAIDYTHRAVVISIRGTLSMKDILTDLNAEGEVLPLQPPRDDWLGHKGMVQAAIYIRNKLQEENLIERALQRNPDRQTHTFDLVLVGHSLGAGTAAILAILLKPEHPTLQCFSYSPPGGLLSMPAVEYSKSFITSVVLGKDVVPRIGLNQMEALRADLINAIQR 553          
BLAST of Sn1-specific diacylglycerol lipase beta vs. Ensembl Fly
Match: inaE (gene:FBgn0261244 transcript:FBtr0340291)

HSP 1 Score: 157.532 bits (397), Expect = 1.505e-39
Identity = 110/421 (26.13%), Postives = 193/421 (45.84%), Query Frame = 2
Query:  638 WKHRLKWILCIKKVNDEERNALDSAANIFASLCEESNLVFSDFEAGIQLLCKQDANAYDFTEYSLKINKSKSGVTNSSSFNQKMYTIINHF-INNEENYVNMSNFKKLANENLIETDSKVNNSEVNLADKISTGNKSDLKSDWRDIRFIYYFSTMAKASYGWPIHLLFHDENKSRLKF------CMGLCCKHSTSCFCNKCICCDCEKSNSNDQKLIYVKERFCSCHEKAILNETHLEKENLVCVSFYNKTFHSPFFIAISHEIEVIILSIRGTFSLKDILTDMDA-NLIEVFEDGFSGYLAHSGMLNAAKNIKRKIQEENIIHNILNDNKN-----YRLIITGHSLGAGIAIILSCFYYHEFPNLKCYAFSPPLGTVNIKTADYCKDFVLSIVNGVEWVPRISLQSIVVAKRRLIECLEN 1861
            W HR + + C    ++  RN+    A + +    E ++V SD  AG+ LL K     +   E    + + K+G           Y  ++   I     ++ +++ K   N +  +T                             I ++Y+    A+ +YGWP++++    N+S++        C G CC                    S+D ++I  ++  C C+  A+     L   ++V  +++     +PFF+AI +    +++SIRGT S+KDILTD++A   +   +     +L H GM+ AA  I+ K+QEEN+I   L  N +     + L++ GHSLGAG A IL+     E P L+C+++SPP G +++   +Y K F+ S+V G + VPRI L  +   +  LI  ++ 
Sbjct:  206 WDHRCRLLFCCMGSSERNRNSFTDIARLLSDFFRELDVVPSDVVAGLVLLRK-----FQRLEREAIVRQRKNGT----------YEFLSGVPITERTQFLALNDAK---NYDFFQTV----------------------------IHYMYF----AQGAYGWPMYVII---NRSKMWHLVPELKCFGCCC------------------GTSDDTEVI--QDNCCLCNYAALKKTLQLGDIDIVYATYHVDVGETPFFVAIDYTHRAVVISIRGTLSMKDILTDLNAEGEVLPLQPPRDDWLGHKGMVQAAIYIRNKLQEENLIERALQRNPDRQTHTFDLVLVGHSLGAGTAAILAILLKPEHPTLQCFSYSPPGGLLSMPAVEYSKSFITSVVLGKDVVPRIGLNQMEALRADLINAIQR 553          
BLAST of Sn1-specific diacylglycerol lipase beta vs. Ensembl Fly
Match: inaE (gene:FBgn0261244 transcript:FBtr0290302)

HSP 1 Score: 159.073 bits (401), Expect = 1.617e-39
Identity = 110/421 (26.13%), Postives = 193/421 (45.84%), Query Frame = 2
Query:  638 WKHRLKWILCIKKVNDEERNALDSAANIFASLCEESNLVFSDFEAGIQLLCKQDANAYDFTEYSLKINKSKSGVTNSSSFNQKMYTIINHF-INNEENYVNMSNFKKLANENLIETDSKVNNSEVNLADKISTGNKSDLKSDWRDIRFIYYFSTMAKASYGWPIHLLFHDENKSRLKF------CMGLCCKHSTSCFCNKCICCDCEKSNSNDQKLIYVKERFCSCHEKAILNETHLEKENLVCVSFYNKTFHSPFFIAISHEIEVIILSIRGTFSLKDILTDMDA-NLIEVFEDGFSGYLAHSGMLNAAKNIKRKIQEENIIHNILNDNKN-----YRLIITGHSLGAGIAIILSCFYYHEFPNLKCYAFSPPLGTVNIKTADYCKDFVLSIVNGVEWVPRISLQSIVVAKRRLIECLEN 1861
            W HR + + C    ++  RN+    A + +    E ++V SD  AG+ LL K     +   E    + + K+G           Y  ++   I     ++ +++ K   N +  +T                             I ++Y+    A+ +YGWP++++    N+S++        C G CC                    S+D ++I  ++  C C+  A+     L   ++V  +++     +PFF+AI +    +++SIRGT S+KDILTD++A   +   +     +L H GM+ AA  I+ K+QEEN+I   L  N +     + L++ GHSLGAG A IL+     E P L+C+++SPP G +++   +Y K F+ S+V G + VPRI L  +   +  LI  ++ 
Sbjct:  206 WDHRCRLLFCCMGSSERNRNSFTDIARLLSDFFRELDVVPSDVVAGLVLLRK-----FQRLEREAIVRQRKNGT----------YEFLSGVPITERTQFLALNDAK---NYDFFQTV----------------------------IHYMYF----AQGAYGWPMYVII---NRSKMWHLVPELKCFGCCC------------------GTSDDTEVI--QDNCCLCNYAALKKTLQLGDIDIVYATYHVDVGETPFFVAIDYTHRAVVISIRGTLSMKDILTDLNAEGEVLPLQPPRDDWLGHKGMVQAAIYIRNKLQEENLIERALQRNPDRQTHTFDLVLVGHSLGAGTAAILAILLKPEHPTLQCFSYSPPGGLLSMPAVEYSKSFITSVVLGKDVVPRIGLNQMEALRADLINAIQR 553          
BLAST of Sn1-specific diacylglycerol lipase beta vs. Ensembl Zebrafish
Match: dagla (diacylglycerol lipase, alpha [Source:ZFIN;Acc:ZDB-GENE-070619-1])

HSP 1 Score: 209.534 bits (532), Expect = 7.582e-56
Identity = 188/747 (25.17%), Postives = 337/747 (45.11%), Query Frame = 2
Query:  104 MPPLIMFGRKWNIATDDFVYLSFWEFLIR--LFIIVAYVYFNFHENMINNCNSKLYQICHFLLSGSLTCNXXXXXXXXXXXXQGLIMEESKRSKIPFCIYIKLFMSFYEAILSIVAFAINYRYSLIPNKFCEQILIPNKIILYCIGPFLTAYLIIFCI------TYDQLGRHGSNVILLATKK------------------TNIWKHRLKWILCIKKVNDEERNALDSAANIFASLCEESNLVFSDFEAGIQLLCKQDANAYDFTEYSLKINKSKSGVTNSSSFNQKMYTIINHFINNEENYVNMSNFKKLANENLIETDSKVNNSEVNLADKISTGNKSDLKSDWRDIRFIYYFSTMAKASYGWPIHLLFHDENKSRLKFCMGLCCKHSTSCFCNKCICCDCEKSNSNDQKLIYVKE-RFCSCHEKAILNE---THLEKENLVCVSFYNKTFHSPFFIAISHEIEVIILSIRGTFSLKDILTDM--DANLIEVFEDGFSGYLAHSGMLNAAKNIKRKIQEENII-----HNILNDNKNYRLIITGHSLGAGIAIILSCFYYHEFPNLKCYAFSPPLGTVNIKTADYCKDFVLSIVNGVEWVPRISLQSIVVAKRRLIECLE--NYPHXXXXXXXXX-XXXXXXXXXXXXXXRWNILNSFERDEKYWENIEI----NTKL--PGQIIHITNIKKLNDPNKSDLINSNRSFYTPIITSSDQFKTVVFHPKMFKDHFPHQMVKRLFNIL 2206
            MP +++F R+W++ +DD V  +F+ F++     ++++ V F    +   +C+  L       L   ++C LI   AIM +S +G I+    R  + + +YI+L +   E + ++V  A   +Y     + C  I   N      +G     +L+IF +      T+D  GR  + V L AT++                   + W  RLK+ +C  +  D + +A    A++FA    + ++V SD  AG+ LL ++               ++K       + N  +  +    +     Y+++ N  ++A                                     + + Y+   A A+YGWP++LL       R   C GLC +  ++C CN  +      S S   + + V+E   C C+  AI  +     L++  +V  S ++  + +PFF+A+ H  + +++SIRGT S KD LTD+  D+  + V E+    +L H GM+ +A+ IK+K+++E I+      ++     +Y L+I GHSLGAG A ILS     ++P+L+CY++SPP G ++    +Y K+FV S+V G + VPRI L  +   +R L+E L+  + P  +      K   K +     +      +  S  R   +  ++ I    +T L  PG++IH+ +    N P +          Y+ +   +  F  V+  P M  +H PH ++  L  +L
Sbjct:    1 MPGMVLFRRRWSVGSDDLVLPAFFLFVLHCIWLVVLSVVLFGLPYSSEQSCSVTLVDHGRGYLGILVSC-LICESAIMWLSMRGSILYTQPREAVQYVLYIRLAILLVELVYAVVGIAWLVQY----YQPCPDITAKN----LALGIVACNWLVIFSVCFTMMCTFDPTGR--TFVKLKATRRRQRNLTTYTLRHRLEEGQASSWSRRLKFFMCCTRAKDTQSDAYSEVASLFAEFFRDLDIVPSDIIAGLVLLRQR--------------QRAKRSAILDQANNDVLAFLSGMPVTRNTKYLDLKNSTEMAM-----------------------------------YKEVCYYMLFAMAAYGWPVYLL-------RKPAC-GLC-RLVSTCSCNTSV------SGSRLSQSVTVEEDNCCGCNVLAIRRQFLDRDLKEVQIVYTSCHDAVYETPFFVAVDHAKKKVVISIRGTLSPKDALTDLTGDSERLPV-EEQHGTWLGHKGMVYSAEYIKKKLEQEMILSQAFGRDLSKGTMHYGLVIVGHSLGAGTAAILSFLLRPQYPSLQCYSYSPPGGLLSEDAMEYSKEFVTSVVLGKDLVPRIGLSQLEGFRRHLLEVLQKSDKPKWRIIAGGTKCIPKSELPMDDEAPVSQGVTPSSSRLWLHPSDLSIALSASTPLFPPGRVIHVVH----NHPPEM-CCGQEEPTYSALWGDNKAFDEVIISPAMLNEHMPHVVMDGLNKVL 666          
BLAST of Sn1-specific diacylglycerol lipase beta vs. Ensembl Zebrafish
Match: dagla (diacylglycerol lipase, alpha [Source:ZFIN;Acc:ZDB-GENE-070619-1])

HSP 1 Score: 209.534 bits (532), Expect = 7.582e-56
Identity = 188/747 (25.17%), Postives = 337/747 (45.11%), Query Frame = 2
Query:  104 MPPLIMFGRKWNIATDDFVYLSFWEFLIR--LFIIVAYVYFNFHENMINNCNSKLYQICHFLLSGSLTCNXXXXXXXXXXXXQGLIMEESKRSKIPFCIYIKLFMSFYEAILSIVAFAINYRYSLIPNKFCEQILIPNKIILYCIGPFLTAYLIIFCI------TYDQLGRHGSNVILLATKK------------------TNIWKHRLKWILCIKKVNDEERNALDSAANIFASLCEESNLVFSDFEAGIQLLCKQDANAYDFTEYSLKINKSKSGVTNSSSFNQKMYTIINHFINNEENYVNMSNFKKLANENLIETDSKVNNSEVNLADKISTGNKSDLKSDWRDIRFIYYFSTMAKASYGWPIHLLFHDENKSRLKFCMGLCCKHSTSCFCNKCICCDCEKSNSNDQKLIYVKE-RFCSCHEKAILNE---THLEKENLVCVSFYNKTFHSPFFIAISHEIEVIILSIRGTFSLKDILTDM--DANLIEVFEDGFSGYLAHSGMLNAAKNIKRKIQEENII-----HNILNDNKNYRLIITGHSLGAGIAIILSCFYYHEFPNLKCYAFSPPLGTVNIKTADYCKDFVLSIVNGVEWVPRISLQSIVVAKRRLIECLE--NYPHXXXXXXXXX-XXXXXXXXXXXXXXRWNILNSFERDEKYWENIEI----NTKL--PGQIIHITNIKKLNDPNKSDLINSNRSFYTPIITSSDQFKTVVFHPKMFKDHFPHQMVKRLFNIL 2206
            MP +++F R+W++ +DD V  +F+ F++     ++++ V F    +   +C+  L       L   ++C LI   AIM +S +G I+    R  + + +YI+L +   E + ++V  A   +Y     + C  I   N      +G     +L+IF +      T+D  GR  + V L AT++                   + W  RLK+ +C  +  D + +A    A++FA    + ++V SD  AG+ LL ++               ++K       + N  +  +    +     Y+++ N  ++A                                     + + Y+   A A+YGWP++LL       R   C GLC +  ++C CN  +      S S   + + V+E   C C+  AI  +     L++  +V  S ++  + +PFF+A+ H  + +++SIRGT S KD LTD+  D+  + V E+    +L H GM+ +A+ IK+K+++E I+      ++     +Y L+I GHSLGAG A ILS     ++P+L+CY++SPP G ++    +Y K+FV S+V G + VPRI L  +   +R L+E L+  + P  +      K   K +     +      +  S  R   +  ++ I    +T L  PG++IH+ +    N P +          Y+ +   +  F  V+  P M  +H PH ++  L  +L
Sbjct:    1 MPGMVLFRRRWSVGSDDLVLPAFFLFVLHCIWLVVLSVVLFGLPYSSEQSCSVTLVDHGRGYLGILVSC-LICESAIMWLSMRGSILYTQPREAVQYVLYIRLAILLVELVYAVVGIAWLVQY----YQPCPDITAKN----LALGIVACNWLVIFSVCFTMMCTFDPTGR--TFVKLKATRRRQRNLTTYTLRHRLEEGQASSWSRRLKFFMCCTRAKDTQSDAYSEVASLFAEFFRDLDIVPSDIIAGLVLLRQR--------------QRAKRSAILDQANNDVLAFLSGMPVTRNTKYLDLKNSTEMAM-----------------------------------YKEVCYYMLFAMAAYGWPVYLL-------RKPAC-GLC-RLVSTCSCNTSV------SGSRLSQSVTVEEDNCCGCNVLAIRRQFLDRDLKEVQIVYTSCHDAVYETPFFVAVDHAKKKVVISIRGTLSPKDALTDLTGDSERLPV-EEQHGTWLGHKGMVYSAEYIKKKLEQEMILSQAFGRDLSKGTMHYGLVIVGHSLGAGTAAILSFLLRPQYPSLQCYSYSPPGGLLSEDAMEYSKEFVTSVVLGKDLVPRIGLSQLEGFRRHLLEVLQKSDKPKWRIIAGGTKCIPKSELPMDDEAPVSQGVTPSSSRLWLHPSDLSIALSASTPLFPPGRVIHVVH----NHPPEM-CCGQEEPTYSALWGDNKAFDEVIISPAMLNEHMPHVVMDGLNKVL 666          
BLAST of Sn1-specific diacylglycerol lipase beta vs. Ensembl Zebrafish
Match: BX248389.1 (diacylglycerol lipase, alpha [Source:NCBI gene;Acc:569398])

HSP 1 Score: 206.068 bits (523), Expect = 7.459e-55
Identity = 185/745 (24.83%), Postives = 326/745 (43.76%), Query Frame = 2
Query:  104 MPPLIMFGRKWNIATDDFVYLSFWEFLIR--LFIIVAYVYFNFHENMINNCNSKLYQICHFLLSGSLTCNXXXXXXXXXXXXQGLIMEESKRSKIPFCIYIKLFMSFYEAILSIVAFAINYRYSLIPNKFCEQILIPNKIILYCIGPFLTAYLIIFCI------TYDQLGRHGSNVILLATKK------------------TNIWKHRLKWILCIKKVNDEERNALDSAANIFASLCEESNLVFSDFEAGIQLLCKQDANAYDFTEYSLKINKSKSGVTNSSSFNQKMYTIINHFINNEENYVNMSNFKKLANENLIETDSKVNNSEVNLADKISTGNKSDLKSDWRDIRFIYYFSTMAKASYGWPIHLLFHDENKSRLKFCMGLCCKHSTSCFCNKCICCDCEKSNSNDQKLIYVKERFCSCHEKAILNE---THLEKENLVCVSFYNKTFHSPFFIAISHEIEVIILSIRGTFSLKDILTDM--DANLIEVFEDGFSGYLAHSGMLNAAKNIKRKIQEENII-----HNILNDNKNYRLIITGHSLGAGIAIILSCFYYHEFPNLKCYAFSPPLGTVNIKTADYCKDFVLSIVNGVEWVPRISLQSIVVAKRRLIECLE--NYPHXXXXXXXXXXXXXXXXXXXXXXXRWNILNSFERDEKYWENIEI----NTKL--PGQIIHITNIKKLNDPNKSDLINSNRSFYTPIITSSDQFKTVVFHPKMFKDHFPHQMVKRLFNIL 2206
            MP +++F R+W++ +DD V  +F+ F++     ++++ V F    +   +C+  L       L G L   LI   AIM +S +G I+    R  + + +YI+L +   E + ++V  A   +Y     + C  I   N      +G     +L+IF +      T+D  GR  + V L AT++                   + W  RLK+ +C  +  D + +A    A++FA    + ++V SD  AG+ LL ++               ++K       + N  +  +    +     Y+++ N  ++A                                     + + Y+   A A+YGWP++LL     K     C  L C            C  C   +   Q +   ++  C C+  AI  +     L++  +V  S ++  + +PFF+A+ H  + +++SIRGT S KD LTD+  D+  + V E+    +L H GM+ +A+ IK+K+++E I+      ++     +Y L+I GHSLGAG A ILS     ++P+L+CY++SPP G ++    +Y K+FV S+V G + VPRI L  +   +R L+E L+  N P  +      K I               +  S  R   +  ++ I    +T L  PG++IH+ +    N P +          Y+ +   +  F  V+  P M  +H PH ++  L  +L
Sbjct:    1 MPGMVLFRRRWSVGSDDLVLPAFFLFVLHCIWLVVLSVVLFGLPYSSEQSCSVTLVDHGRGYL-GILVSCLICESAIMWLSMRGSILYTQPREAVQYVLYIRLAILLVELVYAVVGIAWLVQY----YQPCPDITAKN----LALGIVACNWLVIFSVCFTMMCTFDPTGR--TFVKLKATRRRQRNLTTYTLRHRLEEGQASSWSRRLKFFMCCTRAKDTQSDAYSEVASLFAEFFRDLDIVPSDIIAGLVLLRQR--------------QRAKRSAILDQANNDVLAFLSGMPVTRNTKYLDLKNSTEMAM-----------------------------------YKEVCYYMLFAMAAYGWPVYLL----RKPACGLCRLLRC------------CSACLYVSRLSQSVTVEEDNCCGCNVLAIRRQFLDRDLKEVQIVYTSCHDAVYETPFFVAVDHAKKKVVISIRGTLSPKDALTDLTGDSERLPV-EEQHGTWLGHKGMVYSAEYIKKKLEQEMILSQAFGRDLSKGTMHYGLVIVGHSLGAGTAAILSFLLRPQYPSLQCYSYSPPGGLLSEDAMEYSKEFVTSVVLGKDLVPRIGLSQLEGFRRHLLEVLQKSNKPKWRIIAGGTKCIPKSELPMDDE----GVTPSSSRLWLHPSDLSIALSASTPLFPPGRVIHVVH----NHPPEM-CCGQEEPTYSALWGDNKAFDEVIISPAMLNEHMPHVVMDGLNKVL 659          
BLAST of Sn1-specific diacylglycerol lipase beta vs. Ensembl Zebrafish
Match: BX248389.1 (diacylglycerol lipase, alpha [Source:NCBI gene;Acc:569398])

HSP 1 Score: 204.142 bits (518), Expect = 3.354e-54
Identity = 181/743 (24.36%), Postives = 329/743 (44.28%), Query Frame = 2
Query:  104 MPPLIMFGRKWNIATDDFVYLSFWEFLIR--LFIIVAYVYFNFHENMINNCNSKLYQICHFLLSGSLTCNXXXXXXXXXXXXQGLIMEESKRSKIPFCIYIKLFMSFYEAILSIVAFAINYRYSLIPNKFCEQILIPNKIILYCIGPFLTAYLIIFCI------TYDQLGRHGSNVILLATKK------------------TNIWKHRLKWILCIKKVNDEERNALDSAANIFASLCEESNLVFSDFEAGIQLLCKQDANAYDFTEYSLKINKSKSGVTNSSSFNQKMYTIINHFINNEENYVNMSNFKKLANENLIETDSKVNNSEVNLADKISTGNKSDLKSDWRDIRFIYYFSTMAKASYGWPIHLLFHDE-NKSRLKFCMGLCCKHSTSCFCNKCICCDCEKSNSNDQKLIYVKERFCSCHEKAILNETHLEKENLVCVSFYNKTFHSPFFIAISHEIEVIILSIRGTFSLKDILTDM--DANLIEVFEDGFSGYLAHSGMLNAAKNIKRKIQEENII-----HNILNDNKNYRLIITGHSLGAGIAIILSCFYYHEFPNLKCYAFSPPLGTVNIKTADYCKDFVLSIVNGVEWVPRISLQSIVVAKRRLIECLE--NYPHXXXXXXXXXXXXXXXXXXXXXXXRWNILNSFERDEKYWENIEI----NTKL--PGQIIHITNIKKLNDPNKSDLINSNRSFYTPIITSSDQFKTVVFHPKMFKDHFPHQMVKRLFNIL 2206
            MP +++F R+W++ +DD V  +F+ F++     ++++ V F    +   +C+  L       L   ++C LI   AIM +S +G I+    R  + + +YI+L +   E + ++V  A   +Y     + C  I   N      +G     +L+IF +      T+D  GR  + V L AT++                   + W  RLK+ +C  +  D + +A    A++FA    + ++V SD  AG+ LL ++               ++K       + N  +  +    +     Y+++ N  ++A                                     + + Y+   A A+YGWP++LL        RL  C  +C + S +   + C  C+          ++ ++ +F          +  L++  +V  S ++  + +PFF+A+ H  + +++SIRGT S KD LTD+  D+  + V E+    +L H GM+ +A+ IK+K+++E I+      ++     +Y L+I GHSLGAG A ILS     ++P+L+CY++SPP G ++    +Y K+FV S+V G + VPRI L  +   +R L+E L+  N P  +      K I               +  S  R   +  ++ I    +T L  PG++IH+ +    N P +          Y+ +   +  F  V+  P M  +H PH ++  L  +L
Sbjct:    1 MPGMVLFRRRWSVGSDDLVLPAFFLFVLHCIWLVVLSVVLFGLPYSSEQSCSVTLVDHGRGYLGILVSC-LICESAIMWLSMRGSILYTQPREAVQYVLYIRLAILLVELVYAVVGIAWLVQY----YQPCPDITAKN----LALGIVACNWLVIFSVCFTMMCTFDPTGR--TFVKLKATRRRQRNLTTYTLRHRLEEGQASSWSRRLKFFMCCTRAKDTQSDAYSEVASLFAEFFRDLDIVPSDIIAGLVLLRQR--------------QRAKRSAILDQANNDVLAFLSGMPVTRNTKYLDLKNSTEMAM-----------------------------------YKEVCYYMLFAMAAYGWPVYLLRKPACGLCRLLLC-SMCSRFSVTVEEDNCCGCN----------VLAIRRQFL---------DRDLKEVQIVYTSCHDAVYETPFFVAVDHAKKKVVISIRGTLSPKDALTDLTGDSERLPV-EEQHGTWLGHKGMVYSAEYIKKKLEQEMILSQAFGRDLSKGTMHYGLVIVGHSLGAGTAAILSFLLRPQYPSLQCYSYSPPGGLLSEDAMEYSKEFVTSVVLGKDLVPRIGLSQLEGFRRHLLEVLQKSNKPKWRIIAGGTKCIPKSELPMDDE----GVTPSSSRLWLHPSDLSIALSASTPLFPPGRVIHVVH----NHPPEM-CCGQEEPTYSALWGDNKAFDEVIISPAMLNEHMPHVVMDGLNKVL 653          
BLAST of Sn1-specific diacylglycerol lipase beta vs. Ensembl Zebrafish
Match: BX248389.1 (diacylglycerol lipase, alpha [Source:NCBI gene;Acc:569398])

HSP 1 Score: 204.142 bits (518), Expect = 3.675e-54
Identity = 181/743 (24.36%), Postives = 329/743 (44.28%), Query Frame = 2
Query:  104 MPPLIMFGRKWNIATDDFVYLSFWEFLIR--LFIIVAYVYFNFHENMINNCNSKLYQICHFLLSGSLTCNXXXXXXXXXXXXQGLIMEESKRSKIPFCIYIKLFMSFYEAILSIVAFAINYRYSLIPNKFCEQILIPNKIILYCIGPFLTAYLIIFCI------TYDQLGRHGSNVILLATKK------------------TNIWKHRLKWILCIKKVNDEERNALDSAANIFASLCEESNLVFSDFEAGIQLLCKQDANAYDFTEYSLKINKSKSGVTNSSSFNQKMYTIINHFINNEENYVNMSNFKKLANENLIETDSKVNNSEVNLADKISTGNKSDLKSDWRDIRFIYYFSTMAKASYGWPIHLLFHDE-NKSRLKFCMGLCCKHSTSCFCNKCICCDCEKSNSNDQKLIYVKERFCSCHEKAILNETHLEKENLVCVSFYNKTFHSPFFIAISHEIEVIILSIRGTFSLKDILTDM--DANLIEVFEDGFSGYLAHSGMLNAAKNIKRKIQEENII-----HNILNDNKNYRLIITGHSLGAGIAIILSCFYYHEFPNLKCYAFSPPLGTVNIKTADYCKDFVLSIVNGVEWVPRISLQSIVVAKRRLIECLE--NYPHXXXXXXXXXXXXXXXXXXXXXXXRWNILNSFERDEKYWENIEI----NTKL--PGQIIHITNIKKLNDPNKSDLINSNRSFYTPIITSSDQFKTVVFHPKMFKDHFPHQMVKRLFNIL 2206
            MP +++F R+W++ +DD V  +F+ F++     ++++ V F    +   +C+  L       L   ++C LI   AIM +S +G I+    R  + + +YI+L +   E + ++V  A   +Y     + C  I   N      +G     +L+IF +      T+D  GR  + V L AT++                   + W  RLK+ +C  +  D + +A    A++FA    + ++V SD  AG+ LL ++               ++K       + N  +  +    +     Y+++ N  ++A                                     + + Y+   A A+YGWP++LL        RL  C  +C + S +   + C  C+          ++ ++ +F          +  L++  +V  S ++  + +PFF+A+ H  + +++SIRGT S KD LTD+  D+  + V E+    +L H GM+ +A+ IK+K+++E I+      ++     +Y L+I GHSLGAG A ILS     ++P+L+CY++SPP G ++    +Y K+FV S+V G + VPRI L  +   +R L+E L+  N P  +      K I               +  S  R   +  ++ I    +T L  PG++IH+ +    N P +          Y+ +   +  F  V+  P M  +H PH ++  L  +L
Sbjct:    1 MPGMVLFRRRWSVGSDDLVLPAFFLFVLHCIWLVVLSVVLFGLPYSSEQSCSVTLVDHGRGYLGILVSC-LICESAIMWLSMRGSILYTQPREAVQYVLYIRLAILLVELVYAVVGIAWLVQY----YQPCPDITAKN----LALGIVACNWLVIFSVCFTMMCTFDPTGR--TFVKLKATRRRQRNLTTYTLRHRLEEGQASSWSRRLKFFMCCTRAKDTQSDAYSEVASLFAEFFRDLDIVPSDIIAGLVLLRQR--------------QRAKRSAILDQANNDVLAFLSGMPVTRNTKYLDLKNSTEMAM-----------------------------------YKEVCYYMLFAMAAYGWPVYLLRKPACGLCRLLLC-SMCSRFSVTVEEDNCCGCN----------VLAIRRQFL---------DRDLKEVQIVYTSCHDAVYETPFFVAVDHAKKKVVISIRGTLSPKDALTDLTGDSERLPV-EEQHGTWLGHKGMVYSAEYIKKKLEQEMILSQAFGRDLSKGTMHYGLVIVGHSLGAGTAAILSFLLRPQYPSLQCYSYSPPGGLLSEDAMEYSKEFVTSVVLGKDLVPRIGLSQLEGFRRHLLEVLQKSNKPKWRIIAGGTKCIPKSELPMDDE----GVTPSSSRLWLHPSDLSIALSASTPLFPPGRVIHVVH----NHPPEM-CCGQEEPTYSALWGDNKAFDEVIISPAMLNEHMPHVVMDGLNKVL 653          
BLAST of Sn1-specific diacylglycerol lipase beta vs. Ensembl Xenopus
Match: cxxc4 (CXXC finger protein 4 [Source:Xenbase;Acc:XB-GENE-479477])

HSP 1 Score: 197.978 bits (502), Expect = 7.193e-52
Identity = 178/754 (23.61%), Postives = 318/754 (42.18%), Query Frame = 2
Query:  104 MPPLIMFGRKWNIATDDFVYLSFWEFLIR--LFIIVAYVYFNFHENMINNCNSKLYQICHFLLSGSLTCNXXXXXXXXXXXXQGLIMEESKRSKIPFCIYIKLFMSFYEAILSIVAFAINYRYSLIPNKFCEQILIPNKIILYCIGPFLTAYLIIF--CIT----YDQLGRHGSNVILLATKK------------------TNIWKHRLKWILCIKKVNDEERNALDSAANIFASLCEESNLVFSDFEAGIQLLCKQDANAYDFTEYSLKINKSKSGVTNSSSFNQKMYTIINHFINNEENYVNMSNFKKLANENLIETDSKVNNSEVNLADKISTGNKSDLKSDWRDIRFIYYFSTMAKASYGWPIHLLFHDE----------------NKSRLKFCMGLCCKHSTSCFCNKCICCDCEKSNSNDQKLIYVKERFCSCHEKAILNETHLEKENLVCVSFYNKTFHSPFFIAISHEIEVIILSIRGTFSLKDILTDMDANLIEVFEDGFSG-YLAHSGMLNAAKNIKRKIQEENII-----HNILNDNKNYRLIITGHSLGAGIAIILSCFYYHEFPNLKCYAFSPPLGTVNIKTADYCKDFVLSIVNGVEWVPRISLQSIVVAKRRLIECLE--NYP--HXXXXXXXXXXXXXXXXXXXXXXXRWNILNSFERDEKYWENIEINTKLPGQIIHITNIKKLNDP-NKSDLINSNRSFYTPIITSSDQFKTVVFHPKMFKDHFPHQMVKRLFNIL 2206
            MP +++F R+W++ +DD V    + F++    F+I++ V F    N    C+  L       L   L+C +  +  I +    G++  E  R  + + +Y++L +   E I +IV      +Y       C  +   N      +G  +  +++I   CIT    +D  GR  + V L ATK+                   + W  RLK  LC  +  D + +A    A +FA    + ++V SD  AG+ LL ++               ++K       + N  +  +    +  +  Y+++ N +++                                   +  + + Y+   A A+YGWP++L+ +                    +R +F  G+  +    C CN                 I +K  F        L+E ++   ++V  S ++  + +PFF+A+ H+ + +++SIRGT S KD LTD+  +   +  +G  G +L H GM+ +A+ IK+K+++E ++      ++    K+Y LI+ GHSLGAG A ILS     ++P+LKC+A+SPP G ++    +Y K+FV S+V G + VPRI L  +   +R L++ L+  N P                        +   N L +   D     +       PG+IIH+ +    N P  +    N     Y  I   +  F+ V+  P M  +H PH +++ L  +L
Sbjct:    1 MPGIVVFRRRWSVGSDDLVLPGIFLFVLHSTWFVILSVVLFGLTYNPHETCSLNLVDHGRGYLGILLSCMIAEMAIIWLSMRGGILYTE-PRDSMQYVLYVRLAILVIEFIYAIVGIVWLAQY----YTSCNDVTAKN----VTLGMVVCNWVVILSVCITVLCVFDPTGR--TFVKLRATKRRQRNLRTYNLRHRLEEGQASSWTRRLKVFLCCTRTKDSQSDAYSEIAYLFAEFFRDLDIVPSDIIAGLVLLRQR--------------QRAKRNAVLDEANNDILAFLSGMPVTRKTKYLDLKNAQEM-----------------------------------QRYKEVCYYMLFALAAYGWPMYLIRNPTCGICRLAGACSSCCCLCSARPRFAPGVTIEEDNCCGCNA----------------IAIKRHF--------LDE-NMNMVDIVYTSCHDAVYETPFFVAVDHDKKKVVISIRGTLSPKDALTDLTGDAERLPVEGHHGTWLGHKGMVLSAEYIKKKLEQEMVLSQAFGRDLARGTKHYELIVVGHSLGAGTAAILSFLLRPQYPSLKCFAYSPPGGLLSEDAMEYSKEFVTSVVLGKDLVPRIGLSQLEGFRRHLLDVLQRSNKPKWRIILGGTKCIPKSELPEESEAITIPSNRLWTHPSDLTIALSASTPLYPPGRIIHVVH----NHPVEQCCFWNQEEPTYFAIWGDNKAFEEVIISPAMLHEHLPHVVMEGLNKVL 665          
BLAST of Sn1-specific diacylglycerol lipase beta vs. Ensembl Xenopus
Match: cxxc4 (CXXC finger protein 4 [Source:Xenbase;Acc:XB-GENE-479477])

HSP 1 Score: 195.667 bits (496), Expect = 3.161e-51
Identity = 176/749 (23.50%), Postives = 315/749 (42.06%), Query Frame = 2
Query:  104 MPPLIMFGRKWNIATDDFVYLSFWEFLIR--LFIIVAYVYFNFHENMINNCNSKLYQICHFLLSGSLTCNXXXXXXXXXXXXQGLIMEESKRSKIPFCIYIKLFMSFYEAILSIVAFAINYRYSLIPNKFCEQILIPNKIILYCIGPFLTAYLIIF--CIT----YDQLGRHGSNVILLATKK------------------TNIWKHRLKWILCIKKVNDEERNALDSAANIFASLCEESNLVFSDFEAGIQLLCKQDANAYDFTEYSLKINKSKSGVTNSSSFNQKMYTIINHFINNEENYVNMSNFKKLANENLIETDSKVNNSEVNLADKISTGNKSDLKSDWRDIRFIYYFSTMAKASYGWPIHLLFHDE------------NKSRLKFCMGLCCKHSTSCFCNKCICCDCEKSNSNDQKLIYVKERFCSCHEKAILNETHLEKENLVCVSFYNKTFHSPFFIAISHEIEVIILSIRGTFSLKDILTDMDANLIEVFEDGFSG-YLAHSGMLNAAKNIKRKIQEENII-----HNILNDNKNYRLIITGHSLGAGIAIILSCFYYHEFPNLKCYAFSPPLGTVNIKTADYCKDFVLSIVNGVEWVPRISLQSIVVAKRRLIECLE--NYP--HXXXXXXXXXXXXXXXXXXXXXXXRWNILNSFERDEKYWENIEINTKLPGQIIHITNIKKLNDPNKSDLINSNRSFYTPIITSSDQFKTVVFHPKMFKDHFPHQMVKRLFNIL 2206
            MP +++F R+W++ +DD V    + F++    F+I++ V F    N    C+  L       L   L+C +  +  I +    G++  E  R  + + +Y++L +   E I +IV      +Y       C  +   N      +G  +  +++I   CIT    +D  GR  + V L ATK+                   + W  RLK  LC  +  D + +A    A +FA    + ++V SD  AG+ LL ++               ++K       + N  +  +    +  +  Y+++ N +++                                   +  + + Y+   A A+YGWP++L+ +                +R +F  G+  +    C CN                 I +K  F        L+E ++   ++V  S ++  + +PFF+A+ H+ + +++SIRGT S KD LTD+  +   +  +G  G +L H GM+ +A+ IK+K+++E ++      ++    K+Y LI+ GHSLGAG A ILS     ++P+LKC+A+SPP G ++    +Y K+FV S+V G + VPRI L  +   +R L++ L+  N P                        +   N L +   D     +       PG+IIH+       +P            Y  I   +  F+ V+  P M  +H PH +++ L  +L
Sbjct:    1 MPGIVVFRRRWSVGSDDLVLPGIFLFVLHSTWFVILSVVLFGLTYNPHETCSLNLVDHGRGYLGILLSCMIAEMAIIWLSMRGGILYTE-PRDSMQYVLYVRLAILVIEFIYAIVGIVWLAQY----YTSCNDVTAKN----VTLGMVVCNWVVILSVCITVLCVFDPTGR--TFVKLRATKRRQRNLRTYNLRHRLEEGQASSWTRRLKVFLCCTRTKDSQSDAYSEIAYLFAEFFRDLDIVPSDIIAGLVLLRQR--------------QRAKRNAVLDEANNDILAFLSGMPVTRKTKYLDLKNAQEM-----------------------------------QRYKEVCYYMLFALAAYGWPMYLIRNPTCGICRLAGAFTIVSARPRFAPGVTIEEDNCCGCNA----------------IAIKRHF--------LDE-NMNMVDIVYTSCHDAVYETPFFVAVDHDKKKVVISIRGTLSPKDALTDLTGDAERLPVEGHHGTWLGHKGMVLSAEYIKKKLEQEMVLSQAFGRDLARGTKHYELIVVGHSLGAGTAAILSFLLRPQYPSLKCFAYSPPGGLLSEDAMEYSKEFVTSVVLGKDLVPRIGLSQLEGFRRHLLDVLQRSNKPKWRIILGGTKCIPKSELPEESEAITIPSNRLWTHPSDLTIALSASTPLYPPGRIIHV------EEPT-----------YFAIWGDNKAFEEVIISPAMLHEHLPHVVMEGLNKVL 647          
BLAST of Sn1-specific diacylglycerol lipase beta vs. Ensembl Xenopus
Match: cxxc4 (CXXC finger protein 4 [Source:Xenbase;Acc:XB-GENE-479477])

HSP 1 Score: 190.66 bits (483), Expect = 1.708e-49
Identity = 178/757 (23.51%), Postives = 317/757 (41.88%), Query Frame = 2
Query:  104 MPPLIMFGRKWNIATDDFVYLSFWEFLIR--LFIIVAYVYFNFHENMINNCNSKLYQICHFLLSGSLTCNXXXXXXXXXXXXQGLIMEESKRSKIPFCIYIKL---FMSFYEAILSIVAFAINYRYSLIPNKFCEQILIPNKIILYCIGPFLTAYLIIF--CIT----YDQLGRHGSNVILLATKK------------------TNIWKHRLKWILCIKKVNDEERNALDSAANIFASLCEESNLVFSDFEAGIQLLCKQDANAYDFTEYSLKINKSKSGVTNSSSFNQKMYTIINHFINNEENYVNMSNFKKLANENLIETDSKVNNSEVNLADKISTGNKSDLKSDWRDIRFIYYFSTMAKASYGWPIHLLFHDE----------------NKSRLKFCMGLCCKHSTSCFCNKCICCDCEKSNSNDQKLIYVKERFCSCHEKAILNETHLEKENLVCVSFYNKTFHSPFFIAISHEIEVIILSIRGTFSLKDILTDMDANLIEVFEDGFSG-YLAHSGMLNAAKNIKRKIQEENII-----HNILNDNKNYRLIITGHSLGAGIAIILSCFYYHEFPNLKCYAFSPPLGTVNIKTADYCKDFVLSIVNGVEWVPRISLQSIVVAKRRLIECLE--NYP--HXXXXXXXXXXXXXXXXXXXXXXXRWNILNSFERDEKYWENIEINTKLPGQIIHITNIKKLNDP-NKSDLINSNRSFYTPIITSSDQFKTVVFHPKMFKDHFPHQMVKRLFNIL 2206
            MP +++   +W++ +DD V    + F++    F+I++ V F    N    C+  L       L   L+C +  +  I +    G++  E  R  + + +Y++L    + F  AI+ IV  A  Y         C  +   N      +G  +  +++I   CIT    +D  GR  + V L ATK+                   + W  RLK  LC  +  D + +A    A +FA    + ++V SD  AG+ LL ++               ++K       + N  +  +    +  +  Y+++ N +++                                   +  + + Y+   A A+YGWP++L+ +                    +R +F  G+  +    C CN                 I +K  F        L+E ++   ++V  S ++  + +PFF+A+ H+ + +++SIRGT S KD LTD+  +   +  +G  G +L H GM+ +A+ IK+K+++E ++      ++    K+Y LI+ GHSLGAG A ILS     ++P+LKC+A+SPP G ++    +Y K+FV S+V G + VPRI L  +   +R L++ L+  N P                        +   N L +   D     +       PG+IIH+ +    N P  +    N     Y  I   +  F+ V+  P M  +H PH +++ L  +L
Sbjct:    1 MPGIVVCRCRWSVGSDDLVLPGIFLFVLHSTWFVILSVVLFGLTYNPHETCSLNLVDHGRGYLGILLSCMIAEMAIIWLSMRGGILYTE-PRDSMQYVLYVRLAILVIEFIYAIMGIVWLAQYY-------TSCNDVTAKN----VTLGMVVCNWVVILSVCITVLCVFDPTGR--TFVKLRATKRRQRNLRTYNLRHRLEEGQASSWTRRLKVFLCCTRTKDSQSDAYSEIAYLFAEFFRDLDIVPSDIIAGLVLLRQR--------------QRAKRNAVLDEANNDILAFLSGMPVTRKTKYLDLKNAQEM-----------------------------------QRYKEVCYYMLFALAAYGWPMYLIRNPTCGICRLAGACSSCCCLCSARPRFAPGVTIEEDNCCGCNA----------------IAIKRHF--------LDE-NMNMVDIVYTSCHDAVYETPFFVAVDHDKKKVVISIRGTLSPKDALTDLTGDAERLPVEGHHGTWLGHKGMVLSAEYIKKKLEQEMVLSQAFGRDLARGTKHYELIVVGHSLGAGTAAILSFLLRPQYPSLKCFAYSPPGGLLSEDAMEYSKEFVTSVVLGKDLVPRIGLSQLEGFRRHLLDVLQRSNKPKWRIILGGTKCIPKSELPEESEAITIPSNRLWTHPSDLTIALSASTPLYPPGRIIHVVH----NHPVEQCCFWNQEEPTYFAIWGDNKAFEEVIISPAMLHEHLPHVVMEGLNKVL 665          
BLAST of Sn1-specific diacylglycerol lipase beta vs. Ensembl Xenopus
Match: cxxc4 (CXXC finger protein 4 [Source:Xenbase;Acc:XB-GENE-479477])

HSP 1 Score: 190.66 bits (483), Expect = 1.755e-49
Identity = 172/748 (22.99%), Postives = 315/748 (42.11%), Query Frame = 2
Query:  104 MPPLIMFGRKWNIATDDFVYLSFWEFLIR--LFIIVAYVYFNFHENMINNCNSKLYQICHFLLSGSLTCNXXXXXXXXXXXXQGLIMEESKRSKIPFCIYIKLFMSFYEAILSIVAFAINYRYSLIPNKFCEQILIPNKIILYCIGPFLTAYLIIFCITYDQLGRHGSNVILLATKK------------------TNIWKHRLKWILCIKKVNDEERNALDSAANIFASLCEESNLVFSDFEAGIQLLCKQDANAYDFTEYSLKINKSKSGVTNSSSFNQKMYTIINHFINNEENYVNMSNFKKLANENLIETDSKVNNSEVNLADKISTGNKSDLKSDWRDIRFIYYFSTMAKASYGWPIHLLFHDE----------------NKSRLKFCMGLCCKHSTSCFCNKCICCDCEKSNSNDQKLIYVKERFCSCHEKAILNETHLEKENLVCVSFYNKTFHSPFFIAISHEIEVIILSIRGTFSLKDILTDMDANLIEVFEDGFSG-YLAHSGMLNAAKNIKRKIQEENII-----HNILNDNKNYRLIITGHSLGAGIAIILSCFYYHEFPNLKCYAFSPPLGTVNIKTADYCKDFVLSIVNGVEWVPRISLQSIVVAKRRLIECLE--NYP--HXXXXXXXXXXXXXXXXXXXXXXXRWNILNSFERDEKYWENIEINTKLPGQIIHITNIKKLNDP-NKSDLINSNRSFYTPIITSSDQFKTVVFHPKMFKDHFPHQMVKRLFNIL 2206
            MP +++   +W++ +DD V    + F++    F+I++ V F    N    C+  L       L   L+C +  +  I +    G++  E  R  + + +Y++L +   E I +IV      +Y    N    + +    ++   +   L+  + + C+ +D  GR  + V L ATK+                   + W  RLK  LC  +  D + +A    A +FA    + ++V SD  AG+ LL ++               ++K       + N  +  +    +  +  Y+++ N +++                                   +  + + Y+   A A+YGWP++L+ +                    +R +F  G+  +    C CN                 I +K  F        L+E ++   ++V  S ++  + +PFF+A+ H+ + +++SIRGT S KD LTD+  +   +  +G  G +L H GM+ +A+ IK+K+++E ++      ++    K+Y LI+ GHSLGAG A ILS     ++P+LKC+A+SPP G ++    +Y K+FV S+V G + VPRI L  +   +R L++ L+  N P                        +   N L +   D     +       PG+IIH+ +    N P  +    N     Y  I   +  F+ V+  P M  +H PH +++ L  +L
Sbjct:    1 MPGIVVCRCRWSVGSDDLVLPGIFLFVLHSTWFVILSVVLFGLTYNPHETCSLNLVDHGRGYLGILLSCMIAEMAIIWLSMRGGILYTE-PRDSMQYVLYVRLAILVIEFIYAIVGIVWLAQYYTSCNDVTAKNVTLGMVVCNWVV-ILSVCITVLCV-FDPTGR--TFVKLRATKRRQRNLRTYNLRHRLEEGQASSWTRRLKVFLCCTRTKDSQSDAYSEIAYLFAEFFRDLDIVPSDIIAGLVLLRQR--------------QRAKRNAVLDEANNDILAFLSGMPVTRKTKYLDLKNAQEM-----------------------------------QRYKEVCYYMLFALAAYGWPMYLIRNPTCGICRLAGACSSCCCLCSARPRFAPGVTIEEDNCCGCNA----------------IAIKRHF--------LDE-NMNMVDIVYTSCHDAVYETPFFVAVDHDKKKVVISIRGTLSPKDALTDLTGDAERLPVEGHHGTWLGHKGMVLSAEYIKKKLEQEMVLSQAFGRDLARGTKHYELIVVGHSLGAGTAAILSFLLRPQYPSLKCFAYSPPGGLLSEDAMEYSKEFVTSVVLGKDLVPRIGLSQLEGFRRHLLDVLQRSNKPKWRIILGGTKCIPKSELPEESEAITIPSNRLWTHPSDLTIALSASTPLYPPGRIIHVVH----NHPVEQCCFWNQEEPTYFAIWGDNKAFEEVIISPAMLHEHLPHVVMEGLNKVL 665          
BLAST of Sn1-specific diacylglycerol lipase beta vs. Ensembl Xenopus
Match: polr3c (polymerase (RNA) III (DNA directed) polypeptide C (62kD) [Source:Xenbase;Acc:XB-GENE-993197])

HSP 1 Score: 186.422 bits (472), Expect = 4.110e-49
Identity = 161/616 (26.14%), Postives = 289/616 (46.92%), Query Frame = 2
Query:  104 MPPLIMFGRKWNIATDDFVYLSFWEFLIRLF----IIVAYVYFNFHENMINNCNSKLYQICHFLLSGSLTCNXXXXXXXXXXXXQGLIMEESKRSKIPFCIYIKLFM---SFYEAILSIVAFAINYRYSLIPNKFCEQILIPNKIILYCIGPFLTAYLIIFCIT------YDQLGRH---------------GSNVIL--LATKKTNIWKHRLKWILCIKKVNDEERNALDSAANIFASLCEESNLVFSDFEAGIQLLCKQDANAYDFTEYSLKINKSKSGVTNSSSFNQKMYTIINHFINNEENYVNMSNFKKLANENLIETDSKVNNSEVNLADKISTGNKSDLKSDWRDIRFIYYFSTMAKASYGWPIHLLFHDENKSRLKFCMGLCCKHSTSCFCNKCICCDCEKSNSNDQKLIYVKERFCSCHEKAILNETHLEKENLVCVSFYNKTFHSPFFIAISHEIEVIILSIRGTFSLKDILTDMDANLIEVFEDGFSGYLAHSGMLNAAKNIKRKIQEENIIHNILNDNKNYRLIITGHSLGAGIAIILSCFYYHEFPNLKCYAFSPPLGTVNIKTADYCKDFVLSIVNGVEWVPRISLQSIVVAKRRLIECLEN 1861
            MP ++MF R+W IA+DD V+   +E ++RL     ++V Y     H + +     + Y I   +L  S+ C   ++ AI+ +S QG I     R  +P  +Y++L +    F  A+L  +  + N          C+++++   +IL+ +   + ++ II   T      +D LGR                 S  +L  + T  T +W++R++ + C    ++E R A       F+S+ +  +  FSD +     L   D  A               G+T                ++ E++ V++S   +   E L  + ++            + G+  +++ D    +  +Y    A A+YGWP+++  +           GLC     SC             N   Q  I   +   +CH  +IL  T L+  + + +SF+N+ +  PFF+A+ H+ E I++++RGT SL+D+LTD+ A+   +  +G +G  AH G+  AA  I R++  + I++        Y+L++ GHSLGAG A +L+    + FP LKCYAFSPP G ++   ADY K+F++S++ G + VPR+SL ++   K +++  + N
Sbjct:    1 MPGMLMFARRWAIASDDLVFPGAFELVVRLIWWIGMLVLYSVHKGHFDCVGGAILRSYLIVLIVLLASIIC---VVSAIVYVSMQGTIANPGPRKCMPKLLYVRLTLYVPEFVWAVLGAIWLSDN-------RMQCDRVVV--HVILFAV---IASWAIILLTTIAILLVFDPLGRKKTLYRASDLQNLESSQSEQLLYNVRTASTRVWENRIRLLCCCIGKDNENRVA-------FSSIAKLLSSYFSDTD-----LVPSDIAA---------------GLT---------------LLHQEQDKVDLS---RDPEEVLCHSPTQ------------NPGDSLEMELD----KAAHYMQ-FAAAAYGWPLYVYSNP--------LTGLCKLCGESC------------QNRASQNGIVGGDNL-NCHFGSILQTTGLQDRDFIYISFHNRIYEIPFFVALDHKTESILVAVRGTLSLEDVLTDLSADCENLHIEGVTGSYAHKGITQAASYIYRRLINDGILNQAFTTAPEYKLVVVGHSLGAGAAAVLAIMLRNSFPTLKCYAFSPPGGLLSKDLADYSKNFIVSVIVGKDLVPRLSLPNMEDLKMKILRMVVN 518          
BLAST of Sn1-specific diacylglycerol lipase beta vs. Ensembl Mouse
Match: Dagla (diacylglycerol lipase, alpha [Source:MGI Symbol;Acc:MGI:2677061])

HSP 1 Score: 186.808 bits (473), Expect = 2.429e-48
Identity = 179/751 (23.83%), Postives = 320/751 (42.61%), Query Frame = 2
Query:  104 MPPLIMFGRKWNIATDDFVYLSFWEFLIRL--FIIVAYVYFNFHENMINNCNSKLYQICHFLLSGSLTCNXXXXXXXXXXXXQGLIMEESKRSKIPFCIYIKLFMSFYEAILSIVAFAINYRY-----SLIPNKFCEQILIPNKIILYCIGPFLTAYLIIFCITYDQLGRHGSNVILLATKK------------------TNIWKHRLKWILCIKKVNDEERNALDSAANIFASLCEESNLVFSDFEAGIQLLCKQDANAYDFTEYSLKINKSKSGVTNSSSFNQKMYTIINHFINNEENYVNMSNFKKLANENLIETDSKVNNSEVNLADKISTGNKS--DLKSDWRDIRF--IYYFSTMAKASYGWPIHLLFHDENKSRLKFCMGLCCKHSTSCFCNKCICCDCEKSNSNDQKLIYVKERFCSCHEKAILNETHLEKENLVCV-----SFYNKTFHSPFFIAISHEIEVIILSIRGTFSLKDILTDMDANLIEVFEDGFSG-YLAHSGMLNAAKNIKRKIQEENII-----HNILNDNKNYRLIITGHSLGAGIAIILSCFYYHEFPNLKCYAFSPPLGTVNIKTADYCKDFVLSIVNGVEWVPRISLQSIVVAKRRLIECLENYPHXXXXXXXXXXXXXXXXXXXXXXXRWNILNSFERDEKYWEN-------IEINTKL--PGQIIHITNIKKLNDP-NKSDLINSNRSFYTPIITSSDQFKTVVFHPKMFKDHFPHQMVKRLFNIL 2206
            MP +++F R+W++ +DD V  + + FL+    F+I++ V F    N    C+  L       L   L+C +  +  I +    G++  E  R  + + +Y++L +   E I +IV      +Y      L        +++ N +++      L+  + + C+ +D  GR  + V L ATK+                     W  RLK  LC  +         DS ++ ++ +                        AY F E+   ++   S +       ++      + + +E N                      N+    L+    T N    DLK+    +R+  + Y+   A A+YGWP++L+       R   C GLC           C CC C         +   ++  C C+  AI    H   EN+  V     S ++  + +PF++A+ H+ + +++SIRGT S KD LTD+  +   +  +G  G +L H GM+ +A+ IK+K+++E ++      ++    K+Y LI+ GHSLGAG A ILS     ++P LKC+A+SPP G ++    +Y K+FV ++V G + VPRI L  +   +R+L++ L+     K+++++            +       L S     + W +       +  +T L  PG+IIH+ +    N P  +          Y  I   +  F  V+  P M  +H P+ +++ L  +L
Sbjct:    1 MPGIVVFRRRWSVGSDDLVLPAIFLFLLHTTWFVILSVVLFGLVYNPHEACSLNLVDHGRGYLGILLSCMIAEMAIIWLSMRGGILYTE-PRDSMQYVLYVRLAILVIEFIYAIVGIVWLTQYYTSCNDLTAKNVTLGMVVCNWVVI------LSVCITVLCV-FDPTGR--TFVKLRATKRRQRNLRTYNLRHRLEEGQATSWSRRLKVFLCCTRTK-------DSQSDAYSEI------------------------AYLFAEFFRDLDIVPSDIIAGLVLLRQRQRAKRNAVLDEAN----------------------NDILAFLSGMPVTRNTKYLDLKNSHEMLRYKEVCYYMLFALAAYGWPMYLM-------RKPTC-GLCQ------LARSCSCCLCPARPRFAPGVTIEEDNCCGCNAIAI--RRHFLDENMTAVDIVYTSCHDAVYETPFYVAVDHDKKKVVISIRGTLSPKDALTDLTGDAERLPVEGHRGTWLGHKGMVLSAEYIKKKLEQEMVLSQAFGRDLGRGTKHYGLIVVGHSLGAGTAAILSFLLRPQYPTLKCFAYSPPGGLLSEDAMEYSKEFVTAVVLGKDLVPRIGLSQLEGFRRQLLDVLQRSTKPKWRIIVGATKCIPKSELPEDQVEVTTLAS----TRLWTHPSDLTIALSASTPLYPPGRIIHVVH----NHPAEQCCCCEQEEPTYFAIWGDNKAFNEVIISPAMLHEHLPYVVMEGLNKVL 664          
BLAST of Sn1-specific diacylglycerol lipase beta vs. Ensembl Mouse
Match: Daglb (diacylglycerol lipase, beta [Source:MGI Symbol;Acc:MGI:2442032])

HSP 1 Score: 172.555 bits (436), Expect = 1.147e-44
Identity = 148/607 (24.38%), Postives = 274/607 (45.14%), Query Frame = 2
Query:  104 MPPLIMFGRKWNIATDDFVYLSFWEFLIR-LFIIVAYVYFNFHENMINNCNSKLYQICHFLLSGSLTCNXXXXXXXXXXXXQGLIMEESKRSKIPFCIYIKLFMSFYEAILSIVAFAINYRYSLIPNKFCEQILIPNKIILYCIG-PFLTAYLIIFCITYDQLG---------------RHGSNVIL--LATKKTNIWKHRLKWILCIKKVNDEERNALDSAANIFASLCEESNLVFSDFEAGIQLLCKQDANAYDFTEYSLKINKSKSGVTNSSSFNQKMYTIINHFINNEENYVNMSNFKKLANENLIETDSKVNNSEVNLADKISTGNKSDLKSDWRDIRFIYYFSTMAKASYGWPIHLLFHDENKSRLKFCMGLCCKHSTSCFCNKCICCDCEKSNSNDQKLIYVKERFCSCHEKAILNETHLEKENLVCVSFYNKTFHSPFFIAISHEIEVIILSIRGTFSLKDILTDMDANLIEVFEDG--FSGYLAHSGMLNAAKNIKRKIQEENIIHNILNDNKNYRLIITGHSLGAGIAIILSCFYYHEFPNLKCYAFSPPLGTVNIKTADYCKDFVLSIVNGVEWVPRISLQSIVVAKRRLIECLEN 1861
            MP +++FGR+W++A+DD V+   +E  +R L+ IV+   +  H   ++     L      +L   L   +  +LAI+ +S +G I     R  +   +YI+L +   E + + +  A   +        C++ ++   I    +    + A ++     +D LG                + SN +L  L T   ++W+ R+++  C    +D  R A  S A++F++   +++LV SD  AG  LL +Q  N                      S +++   ++ H     +                 E D++V N                           +++   A A+YGWP+++        R  F  GLC            I  DC ++   +   +   +   +CH  +IL  T L+  + + +SF++K +  PF + + H  E +++++RGT SL+D+LTD+ A   E  E G      +AH G+  AA+ I R++  + I+    +    Y+L++ GHSLGAG A +L+      +P ++ YAFSPP G ++    +Y KDFV+S++ G++ +PR+S+ ++   KRR++  + N
Sbjct:    1 MPGMVLFGRRWSLASDDLVFPGSFELFLRVLWWIVSLTLYLTHRRRLDCPGGVLLSTYLIVLLVLLAVIICTVLAIVCVSMRGTICNPGPRKSMSKLLYIRLALFLPEMVWASLGAAWVAK-----GIQCDRTVVIGIIATVIVSWIVIAATMVTIIFVFDPLGGKMAPYPPCIPEHLDSNSSNRLLTGLKTAAKSVWETRVQFCCCCVGQDDNTRVAFSSTADLFSTYFSDTDLVPSDIAAGFTLLHQQQDNI---------------------SHSREPPEVVTHTPGQPQE---------------TELDAEVENC--------------------------HHYMPFAAAAYGWPLYIY-------RNPF-TGLCR-----------IGGDCCRARDIEYDAVEGDQH--NCHFASILKTTGLQYRDFIHISFHDKVYELPFIVVLDHRKESVVVAVRGTMSLQDVLTDLSAE-SETLELGIELQDCVAHKGIAQAARYIHRRLVNDGILSQAFSVAPEYQLVLVGHSLGAGAAALLAIMLRGAYPQVRAYAFSPPRGLLSKSLYEYSKDFVVSLILGMDVIPRLSVTNMEDLKRRILRVIAN 518          
BLAST of Sn1-specific diacylglycerol lipase beta vs. UniProt/SwissProt
Match: sp|Q9Y4D2|DGLA_HUMAN (Sn1-specific diacylglycerol lipase alpha OS=Homo sapiens OX=9606 GN=DAGLA PE=1 SV=3)

HSP 1 Score: 202.986 bits (515), Expect = 9.007e-53
Identity = 180/766 (23.50%), Postives = 323/766 (42.17%), Query Frame = 2
Query:  104 MPPLIMFGRKWNIATDDFVYLSFWEFLIRL--FIIVAYVYFNFHENMINNCNSKLYQICHFLLSGSLTCNXXXXXXXXXXXXQGLIMEESKRSKIPFCIYIKLFMSFYEAILSIVAFAINYRY-----SLIPNKFCEQILIPNKIILYCIGPFLTAYLIIFCITYDQLGRHGSNVILLATKK------------------TNIWKHRLKWILCIKKVNDEERNALDSAANIFASLCEESNLVFSDFEAGIQLLCKQDANAYDFTEYSLKINKSKSGVTNSSSFNQKMYTIINHFINNEENYVNMSNFKKLANENLIETDSKVNNSEVNLADKISTGNKSDLKSDWRDIRF--IYYFSTMAKASYGWPIHLLFHDENKSRLKFCMGLCCKHSTSCFCNKCICCDCEKSNSNDQKLIYVKERFCSCHEKAILNETHLEKENLVCV-----SFYNKTFHSPFFIAISHEIEVIILSIRGTFSLKDILTDMDANLIEVFEDGFSG-YLAHSGMLNAAKNIKRKIQEENII-----HNILNDNKNYRLIITGHSLGAGIAIILSCFYYHEFPNLKCYAFSPPLGTVNIKTADYCKDFVLSIVNGVEWVPRISLQSIVVAKRRLIECLENYPHXXXXXXXXXXXXXXXXXXXXXXXRWNILNSFER---DEKYWENIEI----NTKL-------------------PGQIIHITNIKKLNDP-NKSDLINSNRSFYTPIITSSDQFKTVVFHPKMFKDHFPHQMVKRLFNIL 2206
            MP +++F R+W++ +DD V  + + FL+    F+I++ V F    N    C+  L       L   L+C +  +  I +    G++  E  R  + + +Y++L +   E I +IV      +Y      L        +++ N +++      L+  + + C+ +D  GR  + V L ATK+                     W  RLK  LC  +  D + +A    A +FA    + ++V SD  AG+ LL ++               ++K       + N  +  +    +     Y+++ N +++                                     +R+  + Y+   A A+YGWP++L+       R   C GLC           C CC C         +   ++  C C+  AI    H   EN+  V     S ++  + +PF++A+ H+ + +++SIRGT S KD LTD+  +   +  +G  G +L H GM+ +A+ IK+K+++E ++      ++    K+Y LI+ GHSLGAG A ILS     ++P LKC+A+SPP G ++    +Y K+FV ++V G + VPRI L  +   +R+L++ L+                        T  +W I+    +     +  E +E+    +T+L                   PG+IIH+ +    N P  +          Y  I   +  F  V+  P M  +H P+ +++ L  +L
Sbjct:    1 MPGIVVFRRRWSVGSDDLVLPAIFLFLLHTTWFVILSVVLFGLVYNPHEACSLNLVDHGRGYLGILLSCMIAEMAIIWLSMRGGILYTE-PRDSMQYVLYVRLAILVIEFIYAIVGIVWLTQYYTSCNDLTAKNVTLGMVVCNWVVI------LSVCITVLCV-FDPTGR--TFVKLRATKRRQRNLRTYNLRHRLEEGQATSWSRRLKVFLCCTRTKDSQSDAYSEIAYLFAEFFRDLDIVPSDIIAGLVLLRQR--------------QRAKRNAVLDEANNDILAFLSGMPVTRNTKYLDLKNSQEM-------------------------------------LRYKEVCYYMLFALAAYGWPMYLM-------RKPAC-GLCQ------LARSCSCCLCPARPRFAPGVTIEEDNCCGCNAIAI--RRHFLDENMTAVDIVYTSCHDAVYETPFYVAVDHDKKKVVISIRGTLSPKDALTDLTGDAERLPVEGHHGTWLGHKGMVLSAEYIKKKLEQEMVLSQAFGRDLGRGTKHYGLIVVGHSLGAGTAAILSFLLRPQYPTLKCFAYSPPGGLLSEDAMEYSKEFVTAVVLGKDLVPRIGLSQLEGFRRQLLDVLQR----------------------STKPKWRIIVGATKCIPKSELPEEVEVTTLASTRLWTHPSDLTIALSASTPLYPPGRIIHVVH----NHPAEQCCCCEQEEPTYFAIWGDNKAFNEVIISPAMLHEHLPYVVMEGLNKVL 663          
BLAST of Sn1-specific diacylglycerol lipase beta vs. UniProt/SwissProt
Match: sp|Q8NCG7|DGLB_HUMAN (Sn1-specific diacylglycerol lipase beta OS=Homo sapiens OX=9606 GN=DAGLB PE=1 SV=2)

HSP 1 Score: 191.045 bits (484), Expect = 5.178e-50
Identity = 151/611 (24.71%), Postives = 282/611 (46.15%), Query Frame = 2
Query:  104 MPPLIMFGRKWNIATDDFVYLSFWEFLIR-LFIIVAYVYFNFHENMINNCNSKL---YQICHFLLSGSLTCNXXXXXXXXXXXXQGLIMEESKRSKIPFCIYIKLFMSFYEAILSIVAFAINYRYSLIPNKFCEQILIPNKIILYCIGPFL--TAYLIIFCITYDQLG---------------RHGSNVIL--LATKKTNIWKHRLKWILCIKKVNDEERNALDSAANIFASLCEESNLVFSDFEAGIQLLCKQDANAYDFTEYSLKINKSKSGVTNSSSFNQKMYTIINHFINNEENYVNMSNFKKLANENLIETDSKVNNSEVNLADKISTGNKSDLKSDWRDIRFIYYFSTMAKASYGWPIHLLFHDENKSRLKFCMGLCCKHSTSCFCNKCICCDCEKSNSNDQKLIYVKERFCSCHEKAILNETHLEKENLVCVSFYNKTFHSPFFIAISHEIEVIILSIRGTFSLKDILTDMDAN--LIEVFEDGFSGYLAHSGMLNAAKNIKRKIQEENIIHNILNDNKNYRLIITGHSLGAGIAIILSCFYYHEFPNLKCYAFSPPLGTVNIKTADYCKDFVLSIVNGVEWVPRISLQSIVVAKRRLIECLEN 1861
            MP +++FGR+W IA+DD V+  F+E ++R L+ I     +  H   ++     L   Y I   +L   + C +  I+ + +   +G I     R  +   +YI+L + F E + + +  A      +     C++ ++ N II   +  ++   A ++   I +D LG                H S+ +L  L T  T++W+ R+K + C    +D  R A  S A +F++   +++LV SD  AG+ LL +Q  N  +                     NQ+   ++ H   + +                 + D+++ N                           +++   A A+YGWP+++                  ++  +  C   I  DC +S + D  L+   +   +CH  +IL+ T L+  + + VSF++K +  PF +A+ H  E +++++RGT SL+D+LTD+ A   +++V E      LAH G+  AA+ + +++  + I+    +    YRL+I GHSLG G A +L+      +P ++CYAFSPP G  +    +Y + F++S+V G + +PR+S+ ++   KRR++  + +
Sbjct:    1 MPGMVLFGRRWAIASDDLVFPGFFELVVRVLWWIGILTLYLMHRGKLDCAGGALLSSYLIVLMILLAVVICTVSAIMCVSM---RGTICNPGPRKSMSKLLYIRLALFFPEMVWASLGAAW-----VADGVQCDRTVV-NGIIATVVVSWIIIAATVVSIIIVFDPLGGKMAPYSSAGPSHLDSHDSSQLLNGLKTAATSVWETRIKLLCCCIGKDDHTRVAFSSTAELFSTYFSDTDLVPSDIAAGLALLHQQQDNIRN---------------------NQEPAQVVCHAPGSSQE---------------ADLDAELENC--------------------------HHYMQFAAAAYGWPLYIY-----------------RNPLTGLCR--IGGDCCRSRTTDYDLVGGDQ--LNCHFGSILHTTGLQYRDFIHVSFHDKVYELPFLVALDHRKESVVVAVRGTMSLQDVLTDLSAESEVLDV-ECEVQDRLAHKGISQAARYVYQRLINDGILSQAFSIAPEYRLVIVGHSLGGGAAALLATMLRAAYPQVRCYAFSPPRGLWSKALQEYSQSFIVSLVLGKDVIPRLSVTNLEDLKRRILRVVAH 518          
BLAST of Sn1-specific diacylglycerol lipase beta vs. UniProt/SwissProt
Match: sp|Q6WQJ1|DGLA_MOUSE (Sn1-specific diacylglycerol lipase alpha OS=Mus musculus OX=10090 GN=Dagla PE=1 SV=2)

HSP 1 Score: 186.808 bits (473), Expect = 1.699e-47
Identity = 179/751 (23.83%), Postives = 320/751 (42.61%), Query Frame = 2
Query:  104 MPPLIMFGRKWNIATDDFVYLSFWEFLIRL--FIIVAYVYFNFHENMINNCNSKLYQICHFLLSGSLTCNXXXXXXXXXXXXQGLIMEESKRSKIPFCIYIKLFMSFYEAILSIVAFAINYRY-----SLIPNKFCEQILIPNKIILYCIGPFLTAYLIIFCITYDQLGRHGSNVILLATKK------------------TNIWKHRLKWILCIKKVNDEERNALDSAANIFASLCEESNLVFSDFEAGIQLLCKQDANAYDFTEYSLKINKSKSGVTNSSSFNQKMYTIINHFINNEENYVNMSNFKKLANENLIETDSKVNNSEVNLADKISTGNKS--DLKSDWRDIRF--IYYFSTMAKASYGWPIHLLFHDENKSRLKFCMGLCCKHSTSCFCNKCICCDCEKSNSNDQKLIYVKERFCSCHEKAILNETHLEKENLVCV-----SFYNKTFHSPFFIAISHEIEVIILSIRGTFSLKDILTDMDANLIEVFEDGFSG-YLAHSGMLNAAKNIKRKIQEENII-----HNILNDNKNYRLIITGHSLGAGIAIILSCFYYHEFPNLKCYAFSPPLGTVNIKTADYCKDFVLSIVNGVEWVPRISLQSIVVAKRRLIECLENYPHXXXXXXXXXXXXXXXXXXXXXXXRWNILNSFERDEKYWEN-------IEINTKL--PGQIIHITNIKKLNDP-NKSDLINSNRSFYTPIITSSDQFKTVVFHPKMFKDHFPHQMVKRLFNIL 2206
            MP +++F R+W++ +DD V  + + FL+    F+I++ V F    N    C+  L       L   L+C +  +  I +    G++  E  R  + + +Y++L +   E I +IV      +Y      L        +++ N +++      L+  + + C+ +D  GR  + V L ATK+                     W  RLK  LC  +         DS ++ ++ +                        AY F E+   ++   S +       ++      + + +E N                      N+    L+    T N    DLK+    +R+  + Y+   A A+YGWP++L+       R   C GLC           C CC C         +   ++  C C+  AI    H   EN+  V     S ++  + +PF++A+ H+ + +++SIRGT S KD LTD+  +   +  +G  G +L H GM+ +A+ IK+K+++E ++      ++    K+Y LI+ GHSLGAG A ILS     ++P LKC+A+SPP G ++    +Y K+FV ++V G + VPRI L  +   +R+L++ L+     K+++++            +       L S     + W +       +  +T L  PG+IIH+ +    N P  +          Y  I   +  F  V+  P M  +H P+ +++ L  +L
Sbjct:    1 MPGIVVFRRRWSVGSDDLVLPAIFLFLLHTTWFVILSVVLFGLVYNPHEACSLNLVDHGRGYLGILLSCMIAEMAIIWLSMRGGILYTE-PRDSMQYVLYVRLAILVIEFIYAIVGIVWLTQYYTSCNDLTAKNVTLGMVVCNWVVI------LSVCITVLCV-FDPTGR--TFVKLRATKRRQRNLRTYNLRHRLEEGQATSWSRRLKVFLCCTRTK-------DSQSDAYSEI------------------------AYLFAEFFRDLDIVPSDIIAGLVLLRQRQRAKRNAVLDEAN----------------------NDILAFLSGMPVTRNTKYLDLKNSHEMLRYKEVCYYMLFALAAYGWPMYLM-------RKPTC-GLCQ------LARSCSCCLCPARPRFAPGVTIEEDNCCGCNAIAI--RRHFLDENMTAVDIVYTSCHDAVYETPFYVAVDHDKKKVVISIRGTLSPKDALTDLTGDAERLPVEGHRGTWLGHKGMVLSAEYIKKKLEQEMVLSQAFGRDLGRGTKHYGLIVVGHSLGAGTAAILSFLLRPQYPTLKCFAYSPPGGLLSEDAMEYSKEFVTAVVLGKDLVPRIGLSQLEGFRRQLLDVLQRSTKPKWRIIVGATKCIPKSELPEDQVEVTTLAS----TRLWTHPSDLTIALSASTPLYPPGRIIHVVH----NHPAEQCCCCEQEEPTYFAIWGDNKAFNEVIISPAMLHEHLPYVVMEGLNKVL 664          
BLAST of Sn1-specific diacylglycerol lipase beta vs. UniProt/SwissProt
Match: sp|Q5YLM1|DGLA_RAT (Sn1-specific diacylglycerol lipase alpha OS=Rattus norvegicus OX=10116 GN=Dagla PE=1 SV=1)

HSP 1 Score: 186.422 bits (472), Expect = 1.927e-47
Identity = 182/751 (24.23%), Postives = 322/751 (42.88%), Query Frame = 2
Query:  104 MPPLIMFGRKWNIATDDFVYLSFWEFLIRL--FIIVAYVYFNFHENMINNCNSKLYQICHFLLSGSLTCNXXXXXXXXXXXXQGLIMEESKRSKIPFCIYIKLFMSFYEAILSIVAFAINYRY-----SLIPNKFCEQILIPNKIILYCIGPFLTAYLIIFCITYDQLGRHGSNVILLATKK------------------TNIWKHRLKWILCIKKVNDEERNALDSAANIFASLCEESNLVFSDFEAGIQLLCKQDANAYDFTEYSLKINKSKSGVTNSSSFNQKMYTIINHFINNEENYVNMSNFKKLANENLIETDSKVNNSEVNLADKISTGNKS--DLKSDWRDIRF--IYYFSTMAKASYGWPIHLLFHDENKSRLKFCMGLCCKHSTSCFCNKCICCDCEKSNSNDQKLIYVKERFCSCHEKAILNETHLEKENLVCV-----SFYNKTFHSPFFIAISHEIEVIILSIRGTFSLKDILTDMDANLIEVFEDGFSG-YLAHSGMLNAAKNIKRKIQEENII-----HNILNDNKNYRLIITGHSLGAGIAIILSCFYYHEFPNLKCYAFSPPLGTVNIKTADYCKDFVLSIVNGVEWVPRISLQSIVVAKRRLIECLENYPHXXXXXXXXXXXXXXXXXXXXXXXRWNILNSFERDEKYWEN-------IEINTKL--PGQIIHITNIKKLNDP-NKSDLINSNRSFYTPIITSSDQFKTVVFHPKMFKDHFPHQMVKRLFNIL 2206
            MP +++F R+W++ +DD V  + + FL+    F+I++ V F    N    C+  L       L   L+C +  +  I +    G++  E  R  + + +Y++L +   E I +IV      +Y      L        +++ N +++      L+  + + C+ +D  GR  + V L ATK+                     W  RLK  LC  +         DS ++ ++ +                        AY F E+   ++   S              II   +        +   ++ A  N +  D   N+    L+    T N    DLK+    +R+  + Y+   A A+YGWP++L+       R   C GLC           C CC C         +   ++  C C+  AI    H   EN+  V     S ++  + +PF++A+ H+ + +++SIRGT S KD LTD+  +   +  +G  G +L H GM+ +A+ IK+K+++E ++      ++    K+Y LI+ GHSLGAG A ILS     ++P LKC+A+SPP G ++    +Y K+FV ++V G + VPRI L  +   +R+L++ L+     K+++++            +       L S     + W +       +  +T L  PG+IIH+ +    N P  +          Y  I   +  F  V+  P M  +H P+ +++ L  +L
Sbjct:    1 MPGIVVFRRRWSVGSDDLVLPAIFLFLLHTTWFVILSVVLFGLVYNPHEACSLNLVDHGRGYLGILLSCMIAEMAIIWLSMRGGILYTE-PRDSMQYVLYVRLAILVIEFIYAIVGIVWLTQYYTSCNDLTAKNVTLGMVVCNWVVI------LSVCITVLCV-FDPTGR--TFVKLRATKRRQRNLRTYNLRHRLEEGQATSWSRRLKVFLCCTRTK-------DSQSDAYSEI------------------------AYLFAEFFRDLDIVPS-------------DIIAGLV--------LLRQRQRAKRNAV-LDEANNDILAFLSGMPVTRNTKYLDLKNSHEMLRYKEVCYYMLFALAAYGWPMYLM-------RKPTC-GLCQ------LARSCSCCLCPARPRFAPGVTIEEDNCCGCNAIAI--RRHFLDENMTAVDIVYTSCHDAVYETPFYVAVDHDKKKVVISIRGTLSPKDALTDLTGDAERLPVEGHRGTWLGHKGMVLSAEYIKKKLEQEMVLSQAFGRDLGRGTKHYGLIVVGHSLGAGTAAILSFLLRPQYPTLKCFAYSPPGGLLSEDAMEYSKEFVTAVVLGKDLVPRIGLSQLEGFRRQLLDVLQRSTKPKWRIIVGATKCIPKSELPEDQVEVTALAS----TRLWTHPSDLTIALSASTPLYPPGRIIHVVH----NHPAEQCCCCEQEEPTYFAIWGDNKAFNEVIISPAMLHEHLPYVVMEGLNKVL 664          
BLAST of Sn1-specific diacylglycerol lipase beta vs. UniProt/SwissProt
Match: sp|Q91WC9|DGLB_MOUSE (Sn1-specific diacylglycerol lipase beta OS=Mus musculus OX=10090 GN=Daglb PE=1 SV=2)

HSP 1 Score: 172.555 bits (436), Expect = 8.024e-44
Identity = 148/607 (24.38%), Postives = 274/607 (45.14%), Query Frame = 2
Query:  104 MPPLIMFGRKWNIATDDFVYLSFWEFLIR-LFIIVAYVYFNFHENMINNCNSKLYQICHFLLSGSLTCNXXXXXXXXXXXXQGLIMEESKRSKIPFCIYIKLFMSFYEAILSIVAFAINYRYSLIPNKFCEQILIPNKIILYCIG-PFLTAYLIIFCITYDQLG---------------RHGSNVIL--LATKKTNIWKHRLKWILCIKKVNDEERNALDSAANIFASLCEESNLVFSDFEAGIQLLCKQDANAYDFTEYSLKINKSKSGVTNSSSFNQKMYTIINHFINNEENYVNMSNFKKLANENLIETDSKVNNSEVNLADKISTGNKSDLKSDWRDIRFIYYFSTMAKASYGWPIHLLFHDENKSRLKFCMGLCCKHSTSCFCNKCICCDCEKSNSNDQKLIYVKERFCSCHEKAILNETHLEKENLVCVSFYNKTFHSPFFIAISHEIEVIILSIRGTFSLKDILTDMDANLIEVFEDG--FSGYLAHSGMLNAAKNIKRKIQEENIIHNILNDNKNYRLIITGHSLGAGIAIILSCFYYHEFPNLKCYAFSPPLGTVNIKTADYCKDFVLSIVNGVEWVPRISLQSIVVAKRRLIECLEN 1861
            MP +++FGR+W++A+DD V+   +E  +R L+ IV+   +  H   ++     L      +L   L   +  +LAI+ +S +G I     R  +   +YI+L +   E + + +  A   +        C++ ++   I    +    + A ++     +D LG                + SN +L  L T   ++W+ R+++  C    +D  R A  S A++F++   +++LV SD  AG  LL +Q  N                      S +++   ++ H     +                 E D++V N                           +++   A A+YGWP+++        R  F  GLC            I  DC ++   +   +   +   +CH  +IL  T L+  + + +SF++K +  PF + + H  E +++++RGT SL+D+LTD+ A   E  E G      +AH G+  AA+ I R++  + I+    +    Y+L++ GHSLGAG A +L+      +P ++ YAFSPP G ++    +Y KDFV+S++ G++ +PR+S+ ++   KRR++  + N
Sbjct:    1 MPGMVLFGRRWSLASDDLVFPGSFELFLRVLWWIVSLTLYLTHRRRLDCPGGVLLSTYLIVLLVLLAVIICTVLAIVCVSMRGTICNPGPRKSMSKLLYIRLALFLPEMVWASLGAAWVAK-----GIQCDRTVVIGIIATVIVSWIVIAATMVTIIFVFDPLGGKMAPYPPCIPEHLDSNSSNRLLTGLKTAAKSVWETRVQFCCCCVGQDDNTRVAFSSTADLFSTYFSDTDLVPSDIAAGFTLLHQQQDNI---------------------SHSREPPEVVTHTPGQPQE---------------TELDAEVENC--------------------------HHYMPFAAAAYGWPLYIY-------RNPF-TGLCR-----------IGGDCCRARDIEYDAVEGDQH--NCHFASILKTTGLQYRDFIHISFHDKVYELPFIVVLDHRKESVVVAVRGTMSLQDVLTDLSAE-SETLELGIELQDCVAHKGIAQAARYIHRRLVNDGILSQAFSVAPEYQLVLVGHSLGAGAAALLAIMLRGAYPQVRAYAFSPPRGLLSKSLYEYSKDFVVSLILGMDVIPRLSVTNMEDLKRRILRVIAN 518          
BLAST of Sn1-specific diacylglycerol lipase beta vs. TrEMBL
Match: A0A3R7PXD3 (Lipase_3 domain-containing protein OS=Penaeus vannamei OX=6689 GN=C7M84_001160 PE=4 SV=1)

HSP 1 Score: 223.016 bits (567), Expect = 1.164e-58
Identity = 166/593 (27.99%), Postives = 279/593 (47.05%), Query Frame = 2
Query:  104 MPPLIMFGRKWNIATDDFVYLSFWEFLIRL-FIIVAYVYFNFHENMINNCNSKLYQICHFLLSGS--LTCNXXXXXXXXXXXXQGLIMEESKRSKIPFCIYIKLFMSFYEAILSIVAF-AINYRYSLIPNKFCEQIL--IPNKIILY---CIGPFLTAYLIIFCITYDQLGR-------HGSNVI---LLATKKTNIWKHRLKWILCIKKVNDEERNALDSAANIFASLCEESNLVFSDFEAGIQLLCKQDANAYDFTEYSLKINKSKSGVTNSSSFNQKMYTIINHFINNEENYVNMSNFKKLANENLIETDSKVNNSEVNLADKISTGNKSDLKSDWRDIRFIYYFSTMAKASYGWPIHLLFHDENKSRLKFCMGLCCKHSTSCFCNKCICCDCEKSNSNDQKLIYVKERFCSCHEKAILNETHLEKENLVCVSFYNKTFHSPFFIAISHEIEVIILSIRGTFSLKDILTDMDANLIEVFEDGFS-GYLAHSGMLNAAKNIKRKIQEENIIHNILNDNKNYRLIITGHSLGAGIAIILSCFYYHEFPNLKCYAFSPPLGTVNIKTADYCKDFVLSIVNGVEWVPRISLQSI 1822
            MP L +F R+W I +DD V     E L+R  ++ +    F  HE   + C    Y +  FL+     L   LI  +A++  S++G IM+ + R  +P  +  ++ +   E     VA+  +   + ++    C   L  +   +++Y    IG  +    ++F    D + R        GS ++    L T    +W+ R +++ C+   ++    A  + A++FASL EES+LV SD  A + LL +      +  E +++ N     ++ SS+                      ++   L +E  +                    ++   + +W  +   Y+F   A ASYGWP  +          +FC   CC       C    C +C    S D      K+  C C+  A+   T L++E++  ++F+NK +  PFF+AI  E E ++++IRGT SL+D +TD+     EV   G   G LAH GM++AAK ++RK++E   +   L     Y ++ITGHSLGAG A++L+     +FPNLKC+AFSPP G  + + A   + FV+S+V G + VPR+S+ S+
Sbjct:    1 MPALEVFNRRWRIGSDDLVIPGIIEVLVRAGWLGLVITLFVHHEADTSRCEQGKYLLRIFLVGSMVILGATLITTVALVYHSSRGTIMDVAPRKHVPNLLITRVILGIPE-----VAWNGMGSMWIILGLVMCHDALSILMKALVIYTWVAIGVIVIGVWLLF----DPMMRPNRQGPNEGSYLVEGGALGTHYIQLWEKRCRFLCCLTSRDENSSEAFKNVADVFASLFEESDLVASDVAAALVLL-RLKRKQEERQEEAIRKNNLHLPLSRSST----------------------ASIGALQSEGALP-------------------HQQSPRPEWMKVGSAYHFMKFALASYGWPWFVYG--------RFCQA-CCSLWKHLHC----CANCRADPSLD-----AKDNCCLCNTAALKAITGLDQESISYITFHNKIYEVPFFVAIDDETEHVVVAIRGTLSLQDAITDLTCQCTEVEGAGLPEGCLAHKGMVHAAKFVQRKLEETRALREGLASRPGYGVVITGHSLGAGTAVVLAALMRPQFPNLKCFAFSPPGGLCSKEFALATQSFVMSVVVGDDLVPRLSMNSL 524          
BLAST of Sn1-specific diacylglycerol lipase beta vs. TrEMBL
Match: A0A0B8RUR9 (Diacylglycerol lipase, beta OS=Boiga irregularis OX=92519 PE=4 SV=1)

HSP 1 Score: 220.32 bits (560), Expect = 7.029e-58
Identity = 187/754 (24.80%), Postives = 328/754 (43.50%), Query Frame = 2
Query:  104 MPPLIMFGRKWNIATDDFVYLSFWEFLIRL-FIIVAYVYFNFHENMINNCNSKLYQICHFLLSGSLTCNXXXXXXXXXXXXQGLIMEESKRSKIPFCIYIKLFMSFYEAILSIVA--FAINYRYSLIPNKFCEQILI----PNKIILYCIGPFLTAYLIIFCITYDQLGRHGSNVILLATK-----------------KTNIWKHRLKWILCIKKVNDEERNALDSAANIFASLCEESNLVFSDFEAGIQLLCKQDANAYDFTEYSLKINKSKSGVTNSSSFNQKMYTIINHFINNEENYVNMSNFKKLANENLIETDSK--VNNSEVNLADKISTGNKSDLKSDWRDIRFIYYFSTMAKASYGWPIHLLFHD-ENKSRLKFCMGLCCKHSTSCFCNKCICCDCEKSNSNDQKLIYVKERFCSCHEKAILNETHLEKENLVCVSFYNKTFHSPFFIAISHEIEVIILSIRGTFSLKDILTDMDANLIEV-FEDGFSGYLAHSGMLNAAKNIKRKIQEENIIHNILNDNKNYRLIITGHSLGAGIAIILSCFYYHEFPNLKCYAFSPPLGTVNIKTADYCKDFVLSIVNGVEWVPRISLQSIVVAKRRLIECL------------------------ENYPHXXXXXXXXXXXXXXXXXXXXXXXRWNILNSFERDEKYWENIEI-NTKLPGQIIHITNIKKLNDPNKSDLINSNRSFYTPIITSSDQFKTVVFHPKMFKDHFPHQMVKRLFNIL 2206
            MP L++FGR+W I +DDFV+   +E  IR+ + I   V ++ H+   N     L      +L   L   +  + +I   S QG I     R  +P  +Y++L +   E + ++V   +  + R +      CE+ +I       I+ + I  F    + IF   +D LGR    +   A++                  T +W+ R++ + C    ND+ R A  S A +F S   +++LV SD  AG+ LL +Q            K+  SK                                     +E LI+  S   V++ EV L +                     ++   A A+YGWP ++L +      +L+   G CC+                  NS+ Q  I +   + + H  +I+  T L+  + + +SF+NK F  PFF+A+ H+ E +++++RGT S +D+LTD+ A+   +  ED     L H G+  AA  I R++  + I++  L+    Y+L++ GHSLG GIA IL+    + +PN++CYAFSPP G ++   ADY K F++S++ G + VPR+S+ ++   KRR+I  +                        +N+P            +      +  + +  +  S + +     + +  +  LPG+IIHI       + N      S+   Y+   +    F +++  PKM  DH+P  +++ L +++
Sbjct:    1 MPGLMLFGRRWAIGSDDFVFPGAFELFIRVTWWIGILVLYSIHKGQFNCTGGGLLNNYLLILLILLALIIAALSSITYTSMQGTISNPGPRKSLPKLLYVRLALYIPELVWAVVGTVWIADKRVN------CERAMIDAIFGTVIVSWVIMVFTLVAVFIF---FDPLGRKSVYLADYASRNLESSQSEQLFCNIKKRATRVWEKRIRLLCCCIVQNDDHRVAFTSIAELFRSYFSDTDLVPSDIAAGLTLLHQQQD----------KVENSKD-----------------------------------PDEVLIQPPSPPAVDDLEVELENAA-------------------HYMKFAVAAYGWPYYVLANPFTGLCKLR---GFCCR------------------NSSAQTEI-IGNDYLNMHFGSIMQMTGLQNRDFIHISFHNKIFEIPFFVALDHQKEAVVVAVRGTLSCEDVLTDLSADCESLTLEDVLDNGLVHKGITQAANYIYRRLINDGILNQALSIAPEYKLVVAGHSLGGGIASILAIMLRNSYPNMRCYAFSPPGGLLSKSLADYTKQFIVSVIVGKDIVPRLSMLNLEDLKRRIIRIVANCNKPKYRILLHGCWYEVFGGKPDNFPTELDGRNQDALTQPLLAEENLMTQQSPLYTSLDDNSPLGSSPQYPHLFLPGKIIHIV------EENSIKGWFSSEVKYSACWSRETAFGSILISPKMITDHWPDTVLRALQSLV 653          
BLAST of Sn1-specific diacylglycerol lipase beta vs. TrEMBL
Match: A0A210PTB0 (Sn1-specific diacylglycerol lipase beta OS=Mizuhopecten yessoensis OX=6573 GN=KP79_PYT05461 PE=4 SV=1)

HSP 1 Score: 220.32 bits (560), Expect = 3.573e-57
Identity = 198/782 (25.32%), Postives = 345/782 (44.12%), Query Frame = 2
Query:  104 MPPLIMFGRKWNIATDDFVYLSFWEFLIRL-FIIVAYVYFNFHENMINNCNSKLYQICHFLLSGSLTC-NXXXXXXXXXXXXQGLIMEESKRSKIPFCIYIKLFMSFYEAILSIVAFAINYRYSLIPNKFCEQILIPNKIILYCIGPFLTAYLIIFCITYDQLG-----RHGSNVILLATKKTNI----------WKHRLKWILCIKKVNDEERNALDSAANIFASLCE--ESNLVFSDFEAGIQLLCKQDANAYDFTEYSLKINKSKSGVTNSSSFNQKMYTIINHFINNEENYVNMSNFKKL-----ANENLIETDSKVNNSEVNLADKISTGNKSDLKSDWRDIRFIYYFSTMAKASYGWPIHLLFHDENKSRLKFCM--------------GLCCKHSTSCFC--------NKCICCDCEKSNSNDQKLIYVKERFCSCHEKAILNETHLEKENLVCVSFYNKTFHSPFFIAISHEIEVIILSIRGTFSLKDILTDMDANLIEVFEDGFSGYLAHSGMLNAAKNIKRKIQEENIIHNILNDNKNYRLIITGHSLGAGIAIILSCFYYHEFPNLKCYAFSPPLGTVNIKTADYCKDFVLSIVNGVEWVPRISL-------QSIVVAKRRLIE-----------------CLENYPHXXXXXXXXXXXXXXXXXXXXXXXRWNILNSFERDEKYWENIEINTKL---PGQIIHITNIKKLNDPNKSDLINSNRSFYTPIITSSDQFKTVVFHPKMFKDHFPHQMVKRLFNILYENQLFYP 2230
            MP LIMFGR+W I +DDFV+    E  +RL ++I   + F+ H+N   NC+       +F+ +  L C  +++ + I+  S QG IM    R ++P  +Y+K+ +S  E  L+ +     + + L        ++     +L C        LI   + +D LG     R    V+  +T++  +          W+ R K++ C    +D   +A    A +     +  E +LV +D  AG+ L+ ++        + SL    S+    N  S +          ++ +  Y +++   ++     A   + +T    +++   +++ +   ++     DW  I+ + ++   A ASYGWP+  +F + +    K C                 C   S S  C        N+  CC C + +          +  C CH  AI   T ++  +L+ VSFYNK +  PFF+AI  + + +++SIRGT SLKD LTD+ A+ + + +D  + +  H G+  AA  I+ ++Q   I+      N+  RL++TGHSLGAG A +L+      FP+L C+ FSPP G ++ K +  C D+V S+V G + VPR+ +        SI+ A RR                    C  +  +S    +    +  +      T  R N+  + E      E   I       PGQ++HI  +    D +KS    ++ +++    T  D F+ ++  P M  DH P  + K L  +  E++ F P
Sbjct:    1 MPGLIMFGRRWGIGSDDFVFPVLAEIFLRLAWLIAMSIVFHIHQNEPQNCSGGTLLQAYFIGALFLLCLTIVLDIIIVYTSMQGSIMYPYPRKRMPRLLYVKIGVSVPE--LAWIILGTYWSFGLASGCVASVVMTVKGAVL-CGWAIAFLLLIGLFVVFDPLGGLRAKRSQLGVLESSTQEQMMTTAQHTAMKTWERRCKFLCCCVSCDDRSHDAFTEIAKLLTDFFQNVELDLVATDIAAGLILVNREQ-------DVSLLTATSERSNDNYGSLS----------LDRKPQYCSLTTEGQVSDTLTAGTRIADTHDHPSSASSRVSNSVPNSDRRPHPQDWMTIKNMSHYMKYAMASYGWPL-FVFANLSLGLCKLCGQCRCSFLQHCPSWDDCCGSGSASVRCEYDDINGGNQSQCCACARDSGEVHY-----DNCCQCHTAAIRKWTDVDDADLLYVSFYNKIYAIPFFVAIDRKEKAVVVSIRGTLSLKDALTDLSADCVCIDQDECADWKCHKGIYQAACYIRDELQNRQILEKAFQQNQGARLVVTGHSLGAGAAALLAILLKPHFPDLICFTFSPPGGLLSHKASSCCSDYVCSVVLGRDLVPRLGICSMEHLKTSILTAIRRCDRPKYQVIASGMWQAVCTLCPGDSANSCGCRIDPPLLSDQGSSVYVTGQR-NVEEAMESAIMQSEQCRITYPTMYPPGQVLHIVEV----DSDKS--CCASPTYFAEWKTPED-FQEILVSPDMLTDHLPDNVWKALVQL--EDKHFNP 746          
BLAST of Sn1-specific diacylglycerol lipase beta vs. TrEMBL
Match: V9KEV6 (Sn1-specific diacylglycerol lipase beta OS=Callorhinchus milii OX=7868 PE=2 SV=1)

HSP 1 Score: 218.394 bits (555), Expect = 4.243e-57
Identity = 192/757 (25.36%), Postives = 321/757 (42.40%), Query Frame = 2
Query:  104 MPPLIMFGRKWNIATDDFVYLSFWEFLIRL--FIIVAYVYFNFHENMINNCNSKL--YQICHFLLSGSLTCNXXXXXXXXXXXXQGLIMEESKRSKIPFCIYIKLFMSFYEAILSIVAFAINYRYSLIPNKFCEQILIPNKIILYCIGPFLTAY-LIIFCITYDQLGRHG--------------SNVIL--LATKKTNIWKHRLKWILCIKKVNDEERNALDSAANIFASLCEESNLVFSDFEAGIQLLCKQDANAYDFTEYSLKINKSKSGVTNSSSFNQKMYTIINHFINNEENYVNMSNFKKLANENLIETDSKVNNSEVNLADKISTGNKSDLKSDWRDIRFIYYFSTMAKASYGWPIHLLFHDENKSRLKFCMGLCCKHSTSCFCN---KCICCDCEKSNSNDQKLIYVKERFCSCHEKAILNETHLEKENLVCVSFYNKTFHSPFFIAISHEIEVIILSIRGTFSLKDILTDMDANLIEVFEDGFS-GYLAHSGMLNAAKNIKRKIQEENIIHNILNDNKNYRLIITGHSLGAGIAIILSCFYYHEFPNLKCYAFSPPLGTVNIKTADYCKDFVLSIVNGVEWVPRISLQSIVVAKRRLIECL------------------------ENYPHXXXXXXXXXXXXXXXXXXXXXXXRWNILNS-FERDEKYWENIEINTKLPGQIIHIT---NIKKLNDPNKSDLINSNRSFYTPIITSSDQFKTVVFHPKMFKDHFPHQMVKRLFNILYEN 2215
            MP L++FGR+W +A+DD V+   +E LIR+  +I +   YF + E        +L  Y +   +L   +  N  II+ + +   QG I     R  IP  IYI+  +   E I  I+        SL     C   LI   I+   +   +  + L+   + +D LG+                S+ +L    T    +W+HRLK + C    +DE R A       F+SL E    +F  F                F +  L  +   +G++                ++ E++ +  +           E +   +N  V      +   + D++ D        ++   A A+YGWPI++                   +  + FCN    C CC    + S+      V      CH  +++  T L+  + + +SF+NK +  PF++A+ H+ E +++++RGT SL+D+LTD+      +  DG S G  AH G+L+AA  I RK+  + I+         Y+L++TGHSLGAG A IL+   +  +P LKCYAFSPP G ++   ADY KDF++S++ G ++VPR+SL ++   KRR++  +                        +N+P        +   +      S    R +  NS  +       +      LPG+IIHI    N+     P            Y  + + S  F +++  PK+  DH P  ++K L ++  E 
Sbjct:    1 MPGLVVFGRRWALASDDLVFPGAFELLIRVGWWIGILVFYFTYKEQFECKGGEQLRNYLLVLMVLLAGIILNSCIIVGVSM---QGTITNPGPRRAIPTLIYIRAILYLPEVIWGILGIIWVSDESLD----CSSTLIVGVILAIVVSWIILFFTLVAVLVVFDPLGKPKKFEYYLNHNLESSESDQLLYKARTVAARVWEHRLKLLCCCIMQDDEVRTA-------FSSLGE----LFGGF----------------FMDTDLVPSDIAAGLS---------------LLHQEQDKIEQTR----------EPEVVCHNPAVA-----TVPEELDMELD-----NAAHYMLFAVAAYGWPIYVF-----------------TNPLTGFCNLSRDCRCCISPAAESD-----IVGGDHLGCHLTSMIKITGLQYRDFIHISFHNKIYEIPFYVALDHKTEAVLVAVRGTLSLRDVLTDLSTECENLNIDGMSEGCFAHKGILHAASYIYRKLVTDGILSQAFVIAPEYKLVVTGHSLGAGAATILAIMLHKSYPGLKCYAFSPPGGLLSKTLADYTKDFIISVIVGKDFVPRLSLCNMEDLKRRILRIVANCNRPKYQILLRGCWYEIFGGDPDNFPTEMESRRQETLTRPLLADHSLMGHRSSSYNSLLDGSPPISPSKNYPLFLPGKIIHIQEDYNVGSFCRPEIR---------YRAVWSESSAFNSILISPKIISDHMPDIVLKALTSLTREE 657          
BLAST of Sn1-specific diacylglycerol lipase beta vs. TrEMBL
Match: A0A2T7PVV3 (Lipase_3 domain-containing protein OS=Pomacea canaliculata OX=400727 GN=C0Q70_00146 PE=4 SV=1)

HSP 1 Score: 218.394 bits (555), Expect = 1.221e-56
Identity = 187/748 (25.00%), Postives = 327/748 (43.72%), Query Frame = 2
Query:  104 MPPLIMFGRKWNIATDDFVYLSFWEFLIRL-FIIVAYVYFNFHENMINNCNSKLYQICHFLLSGS---LTCNXXXXXXXXXXXXQGLIMEESKRSKIPFCIYIKLFMSFYEAILSIVA--FAINYRYSLIPNKFCEQILIPNKIILYCIGPFLTAYLIIF------CITYDQLG-------------RHGSNVILLATKKTN--IWKHRLKWILCIKKVNDEERNALDSAANIFASLCEESNLVFSDFEAGIQLLCKQDANAYDFTEYSLKINKSKSGVTNSSSFNQKMYTIINHFINNEENYVNMSNFKKLANENLIETDSKVNNSEVNLADKISTGNKSDLKSDWRDIRFIYYFSTMAKASYGWPIHLLFHDENKSRLKFCMGLCCKHSTSCFCNKCICCDCEKSNSNDQKLIYVKERFCSCHEKAILNETHLEKENLVCVSFYNKTFHSPFFIAISHEIEVIILSIRGTFSLKDILTDMDANLIEVFEDGFSGYLAHSGMLNAAKNIKRKIQEENIIHNILND-NKNYRLIITGHSLGAGIAIILSCFYYHEFPNLKCYAFSPPLGTVNIKTADYCKDFVLSIVNGVEWVPRISLQSIVVAKRRLIE--CLEN------------------YPHXXXXXXXXXXXXXXXXXXXXXXXRWNILNSFERDEKYWENI---EINTKLPGQIIHITNIKKLNDPNKSDLINSNRSFYTPIITSSDQFKTVVFHPKMFKDHFPHQMVKRL 2194
            MP +I   R+W IA+DDFVY SF E   R  +++   + F  H    N  N  L +   +  +GS   L+  +++  A+M +S +G I  +  R  +   +Y+KL +   EAI   VA  +   + YS      CE       +     G  + A+++ F       I +D +G             R  S  ++  +K  N  +W+ R + + C    N E  NA    + + A   ++++LV +D  AG+ L+ ++  +                         +K+  +  H                  + N   + + + N+ V++  + S  N+   KS W  +  + ++   A  SYGWP  +  H        F  G+C        C  C CC C ++  +      V +  C CH  AI N T ++ ++L+ VSF++K    PF++A+      +++SIRGT SL+D LTD+ A  + +  DG      HS ML  A+ +K +++   ++    +   +  RL I GHSLGAG+A IL+      +PN+ C+A+SPP G +++  + Y +DFV S+V G + +PR+S+ ++   K +L+   C  N                   PH      +++         ++ S    +  +    E Y + +   E     PGQI+HIT        ++   + S    YT + ++ + F +VV   +M  DHFP  +++ L
Sbjct:    1 MPGIIFLRRRWAIASDDFVYPSFVELCFRSSWLLAISILFGIHHGSFNCDNGYLLR---YYYTGSIILLSFGIVLTAAMMWLSMKGSIRNDYARRHLNIILYLKLLLLLPEAIWITVATYWTFGFSYS------CEL-----TVYWTARGAVICAWIVGFWMFIGILIVFDPMGSVEQHMDLSETSSRKSSKYLIANSKSVNARVWEQRCRCLCCCISSNQEIDNAFADVSKLVADFFKDTDLVATDIAAGLVLVGQEQRHQ-----------------------KEKLTAVAVH------------------SANAGRSPTTIENNRVDVM-RASYSNRGQQKS-WMTLPMMTHYMKFALGSYGWPFFMYNH--------FTTGVCR------LCIACRCCACVRAPKD-----VVSDNICHCHTAAIKNLTKIKDQDLIYVSFHDKYLEIPFYVAVDRRFSSVVVSIRGTLSLQDALTDLSAKGVPLEIDGVPDAFCHSAMLRCAEYVKAELENRQLLEKAFSSLEEGSRLFIVGHSLGAGVAAILTILLKSSYPNIACFAYSPPGGLMSLSASHYTQDFVCSVVVGDDIIPRLSMPNMCYLKIQLLRALCTSNTPKYKILASGCFRILCGCTPHFVSSSSLEEESPLTPQLQAQYSTMIPVQKALLLAEDYAKEMAAAEWQLFPPGQILHITE-------DEEGRLCSGEPQYTAVWSTPEAFSSVVISSRMIMDHFPDVVLQAL 665          
BLAST of Sn1-specific diacylglycerol lipase beta vs. Ensembl Cavefish
Match: dagla (diacylglycerol lipase alpha [Source:NCBI gene;Acc:103028196])

HSP 1 Score: 196.438 bits (498), Expect = 1.468e-51
Identity = 186/770 (24.16%), Postives = 329/770 (42.73%), Query Frame = 2
Query:  104 MPPLIMFGRKWNIATDDFVYLSFWEFLIRL--FIIVAYVYFNFHENMINNCNSKLYQICHFLLSGSLTCNXXXXXXXXXXXXQGLIMEESKRSKIPFCIYIKLFMSFYEAILSIVAFAINYRYSLIPNKFCEQILIPNKIILYCIGPFLTAYLIIFCI------TYDQLGRHGSNVILLATKK------------------------------------------TNIWKHRLKWILCIKKVNDEERNALDSAANIFASLCEESNLVFSDFEAGIQLLCKQDANAYDFTEYSLKINKSKSGVTNSSSFNQKMYTIINHFINNEENYVNMSNFKKLANENLIETDSKVNNSEVNLADKISTGNKSDLKSDWRDIRFIYYFSTMAKASYGWPIHLLFHDENKSRLKFCMGLCCKHSTSCFCNKCICCDCEKSNSNDQKLIYVKE-RFCSCHEKAILNE---THLEKENLVCVSFYNKTFHSPFFIAISHEIEVIILSIRGTFSLKDILTDM--DANLIEVFEDGFSGYLAHSGMLNAAKNIKRKIQEENII-----HNILNDNKNYRLIITGHSLGAGIAIILSCFYYHEFPNLKCYAFSPPLGTVNIKTADYCKDFVLSIVNGVEWVPRISLQSIVVAKRRLIECLE--NYPHXXXXXXXXXXXXXXXXXXXXXXXRWNILNSFERDEKYWENIEINTK------LPGQIIHITNIKKLNDPNKSDLINSNRSFYTPIITSSDQFKTVVFHPKMFKDHFPHQMVKRLFNIL 2206
            MP ++MF R+W++ +DD V  + + F++     ++++ V F        +C+  L       L   ++C LI   AIM +S +G I+    R  + + +YI+L +   E + ++V  A   +Y     + C      N      +G  +  +L+IF +      T+D  GR  + V L AT++                                           + W  RL++ +C  +  D + +A    A++FA    + ++V SD  AG+ LL ++               ++K         N  +  +    +     Y+++ N  ++A                                     + + Y+   A A+YGWP++LL       R   C GLC +  +SC C          S S   + + V+E   C C+  AI  +     L++  +V  S ++  + +PFF+A+ H  + +++SIRGT S KD LTD+  D+  + V E+    +L H GM+ +A+ IK+K+++E I+      ++     +Y L+I GHSLGAG A ILS     ++P L CY++SPP G ++    +Y K+FV S+V G + VPRI L  +   +R L+E L+  + P  +  +   K I               +  S  R   +  ++ I         LPG+IIH+ +    N P ++         Y+ +   +  F  V+  P M  +H PH +++ L  +L
Sbjct:    1 MPGMVMFRRRWSVGSDDLVLPALFLFILHCIWLVVLSVVLFGLPYGSDESCSVTLVDHGRGYLGILVSC-LICESAIMWLSMRGSILYTQPREAVQYVLYIRLAILLVELVYAVVGIAWLVQY----YQPCSDDTAKN----LALGIVVCNWLVIFSVCFTMMCTFDPTGR--TFVKLKATRRRQRNLTTYTLREIPNLCWTLALQDRSYTPLTLSLRHRLEEGQASSWSRRLRFFMCCTRAQDSQSDAYSEVASLFAEFFRDLDIVPSDIIAGLVLLRQR--------------QRAKRSAILDQPNNDILAFLSGMPVTRSTRYLDLKNSTEMAM-----------------------------------YKEVCYYMLFAMAAYGWPMYLL-------RKPAC-GLC-RLLSSCTCTS-------GSGSRLSQSVTVEEDNCCGCNVLAIRRQFLDRDLKQVQIVYTSCHDAVYETPFFVAVDHGKKKVVISIRGTLSPKDALTDLTGDSERLPV-EEQHGVWLGHKGMVYSAEYIKKKLEQEMILSQAFGRDLSKGTMHYGLVIVGHSLGAGTAAILSFLLRPQYPTLHCYSYSPPGGLLSEDAMEYSKEFVTSVVLGKDLVPRIGLSQLEGFRRHLLEVLQKSDKPKWRIIVGGTKCIPKTELPMEDDPPSQGVTPSNSRLCLHPSDLSIALSASTPLYLPGRIIHVVH----NHPPET-CCGQEDPTYSALWGDNKAFDEVIISPAMLNEHMPHVVMEGLNKVL 688          
BLAST of Sn1-specific diacylglycerol lipase beta vs. Ensembl Cavefish
Match: dagla (diacylglycerol lipase alpha [Source:NCBI gene;Acc:103028196])

HSP 1 Score: 191.43 bits (485), Expect = 7.504e-50
Identity = 187/773 (24.19%), Postives = 328/773 (42.43%), Query Frame = 2
Query:  104 MPPLIMFGRKWNIATDDFVYLSFWEFLIRLF--IIVAYVY---------------------------FNFHENMINNCNSKLYQICHFLLSGSLTCNXXXXXXXXXXXXQGLIMEESKRSKIPFCIYIKLFMSFYEAILSIVAFAINYRYSLIPNKFCEQILIPNKIILYCIGPFLTAYLIIFCI------TYDQLGRHGSNVILLATKK------------------TNIWKHRLKWILCIKKVNDEERNALDSAANIFASLCEESNLVFSDFEAGIQLLCKQDANAYDFTEYSLKINKSKSGVTNSSSFNQKMYTIINHFINNEENYVNMSNFKKLANENLIETDSKVNNSEVNLADKISTGNKSDLKSDWRDIRFIYYFSTMAKASYGWPIHLLFHDENKSRLKFCMGLCCKHSTSCFCNKCICCDCEKSNSNDQKLIYVKE-RFCSCHEKAILNE---THLEKENLVCVSFYNKTFHSPFFIAISHEIEVIILSIRGTFSLKDILTDM--DANLIEVFEDGFSGYLAHSGMLNAAKNIKRKIQEENII-----HNILNDNKNYRLIITGHSLGAGIAIILSCFYYHEFPNLKCYAFSPPLGTVNIKTADYCKDFVLSIVNGVEWVPRISLQSIVVAKRRLIECLE--NYPHXXXXXXXXXXXXXXXXXXXXXXXRWNILNSFERDEKYWENIEINTK------LPGQIIHITNIKKLNDPNKSDLINSNRSFYTPIITSSDQFKTVVFHPKMFKDHFPHQMVKRLFNIL 2206
            MP ++MF R+W++ +DD V  + + F++     + V  V+                           F        +C+  L       L   ++C LI   AIM +S +G I+    R  + + +YI+L +   E + ++V  A   +Y     + C      N      +G  +  +L+IF +      T+D  GR  + V L AT++                   + W  RL++ +C  +  D + +A    A++FA    + ++V SD  AG+ LL ++               ++K         N  +  +    +     Y+++ N  ++A                                     + + Y+   A A+YGWP++LL       R   C GLC +  +SC C          S S   + + V+E   C C+  AI  +     L++  +V  S ++  + +PFF+A+ H  + +++SIRGT S KD LTD+  D+  + V E+    +L H GM+ +A+ IK+K+++E I+      ++     +Y L+I GHSLGAG A ILS     ++P L CY++SPP G ++    +Y K+FV S+V G + VPRI L  +   +R L+E L+  + P  +  +   K I               +  S  R   +  ++ I         LPG+IIH+ +    N P ++         Y+ +   +  F  V+  P M  +H PH +++ L  +L
Sbjct:    1 MPGMVMFRRRWSVGSDDLVLPALFLFILHCIWSVTVTGVWGLTDYSLRKAGLRLVSVRRLVVLSVVLFGLPYGSDESCSVTLVDHGRGYLGILVSC-LICESAIMWLSMRGSILYTQPREAVQYVLYIRLAILLVELVYAVVGIAWLVQY----YQPCSDDTAKN----LALGIVVCNWLVIFSVCFTMMCTFDPTGR--TFVKLKATRRRQRNLTTYTLRHRLEEGQASSWSRRLRFFMCCTRAQDSQSDAYSEVASLFAEFFRDLDIVPSDIIAGLVLLRQR--------------QRAKRSAILDQPNNDILAFLSGMPVTRSTRYLDLKNSTEMAM-----------------------------------YKEVCYYMLFAMAAYGWPMYLL-------RKPAC-GLC-RLLSSCTCTS-------GSGSRLSQSVTVEEDNCCGCNVLAIRRQFLDRDLKQVQIVYTSCHDAVYETPFFVAVDHGKKKVVISIRGTLSPKDALTDLTGDSERLPV-EEQHGVWLGHKGMVYSAEYIKKKLEQEMILSQAFGRDLSKGTMHYGLVIVGHSLGAGTAAILSFLLRPQYPTLHCYSYSPPGGLLSEDAMEYSKEFVTSVVLGKDLVPRIGLSQLEGFRRHLLEVLQKSDKPKWRIIVGGTKCIPKTELPMEDDPPSQGVTPSNSRLCLHPSDLSIALSASTPLYLPGRIIHVVH----NHPPET-CCGQEDPTYSALWGDNKAFDEVIISPAMLNEHMPHVVMEGLNKVL 691          
BLAST of Sn1-specific diacylglycerol lipase beta vs. Ensembl Cavefish
Match: daglb (diacylglycerol lipase beta [Source:NCBI gene;Acc:103025334])

HSP 1 Score: 166.007 bits (419), Expect = 1.786e-42
Identity = 110/394 (27.92%), Postives = 189/394 (47.97%), Query Frame = 2
Query: 1100 AKASYGWPIHLLFHDENKSRLKFCMGLCCKHSTSCFCNKC-ICCDCEKSNSNDQKLIYVKERFCSCHEKAILNETHLEKENLVCVSFYNKTFHSPFFIAISHEIEVIILSIRGTFSLKDILTDMDANLIEVFEDGFSGY-LAHSGMLNAAKNIKRKIQEENIIHNILNDNKNYRLIITGHSLGAGIAIILSCFYYHEFPNLKCYAFSPPLGTVNIKTADYCKDFVLSIVNGVEWVPRISLQSIVVAKRRLIECLEN------------------------YPHXXXXXXXXXXXXXXXXXXXXXXXRWNILNSFERDEKYWENIEINTKLPGQIIHITNIKKLNDPNKSDLINSNRSFYTPIITSSDQFKTVVFHPKMFKDHFPHQMVKRLFNI 2203
            A A+YGWP+++                     ++ F   C +  DC ++   +  L  V      C+  +IL+ T L+  + + +SF+N+ +  PFF+A+ H+ E +++++RGT SLKD LTD+ A    +  +G SG   AH G+  AA  I +K+  + I+    +    YRL+ITGHSLGAG A +L+      +P L+CYAFSPP G ++   A+Y K FV+S+V G + VPR+S+ ++   KRR+++ + N                        +P        ++  +      S          S   D+    +  +   LPG+I++IT     + P +    +  R  Y    +S   F++V+  P+M  DH P  +++ L ++
Sbjct:  289 AVAAYGWPLYVY--------------------SNPFTGLCKLSGDCCRNPRAEYDL--VGGDNLGCNFNSILHVTGLQYRDFIHISFHNQIYEIPFFVALDHKREAVLVAVRGTLSLKDALTDLSAECENLPVEGVSGACYAHKGISQAANYIYKKLVNDGILSQAFSIAPEYRLVITGHSLGAGTASLLAVLLRSTYPTLQCYAFSPPGGLMSKALAEYSKQFVVSVVLGKDLVPRLSIPNMEDLKRRILKMVSNCSKPKYKILLHGCWYEVFGGTPDDFPTEMENRREEELSQPLLGGGSLLVQGSTSYQSLSSDDSPTHSTHLPLYLPGRIMYITE----DGPTRRHCFSQVR--YRAEWSSEMAFRSVLISPRMIADHMPDVVLRALHSL 654          
BLAST of Sn1-specific diacylglycerol lipase beta vs. Ensembl Sea Lamprey
Match: daglb (diacylglycerol lipase, beta [Source:ZFIN;Acc:ZDB-GENE-070619-2])

HSP 1 Score: 155.992 bits (393), Expect = 2.684e-40
Identity = 80/256 (31.25%), Postives = 144/256 (56.25%), Query Frame = 2
Query: 1085 YFSTMAKASYGWPIHLLFHDENKSRLKFCMGLCCKHSTSCFCNKCICCDCEKSNSNDQKLIYVKERFCSCHEKAILNETHLEKENLVCVSFYNKTFHSPFFIAISHEIEVIILSIRGTFSLKDILTDMDANLIEVFEDGFS-GYLAHSGMLNAAKNIKRKIQEENIIHNILNDNKNYRLIITGHSLGAGIAIILSCFYYHEFPNLKCYAFSPPLGTVNIKTADYCKDFVLSIVNGVEWVPRISLQSIVVAKRRLIE 1849
            +++  A A+YGWP+++L +  +   + F                    +C    S +  L+   +     +   +L  T L + +L+ VSF+N  +  PFF+A+ H+++ +++S+RGT SL+D+LTD+ A+   +  DG      AH GM+ AA  I +++  + I++      ++Y L++TGHS+G G+A++LS      +P L+CYAFSPP G ++   A Y ++FVLS+V G + V R+SL ++   K+ +I+
Sbjct:  205 HYAKFAWAAYGWPMYVLQNPLSGLSVFFE-------------------NCRGQQSAEYSLVGGGDGGDGRNIATVLRVTGLCRSDLIHVSFHNAVYQIPFFVALDHDMQAVVVSVRGTLSLRDVLTDLSADCESMQVDGLQQDCYAHKGMIQAATFIHQRLVNDGILNEAFQHAQDYHLVVTGHSMGGGVAVLLSILLRCAYPRLRCYAFSPPGGLLSQTLAHYTREFVLSVVVGKDVVARLSLSNMEDLKQSIIQ 441          
BLAST of Sn1-specific diacylglycerol lipase beta vs. Ensembl Sea Lamprey
Match: ENSPMAT00000003941.1 (pep scaffold:Pmarinus_7.0:GL487229:269:2582:1 gene:ENSPMAG00000003605.1 transcript:ENSPMAT00000003941.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 80.1073 bits (196), Expect = 3.464e-17
Identity = 32/74 (43.24%), Postives = 53/74 (71.62%), Query Frame = 2
Query: 1601 NKNYRLIITGHSLGAGIAIILSCFYYHEFPNLKCYAFSPPLGTVNIKTADYCKDFVLSIVNGVEWVPRISLQSI 1822
             + YR+++TGHSLGAG A IL+      +P+L+CYAFSPP G ++   + + + F++S+V G++ VPR+SL ++
Sbjct:    2 GQEYRVVVTGHSLGAGTAAILALLLKPSYPSLRCYAFSPPGGLLSEALSKHAQQFIISVVLGLDLVPRLSLANM 75          
BLAST of Sn1-specific diacylglycerol lipase beta vs. Ensembl Nematostella
Match: EDO43710 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RXR6])

HSP 1 Score: 203.756 bits (517), Expect = 7.251e-56
Identity = 162/610 (26.56%), Postives = 278/610 (45.57%), Query Frame = 2
Query:  104 MPPLIMFGRKWNIATDDFVYLSFWEFLIR--LFIIVAYVYFNFH-----ENMINNCNSKLYQICHFLLSGSLT---CNXXXXXXXXXXXXQGLIMEESKRSKIPFCIYIKLFMSFYEAILSIVAFAINYRYSLIPNKFC---EQILIPNKIILYCIGPFLTAYLIIFCITYDQLGR---------HGSNVI--------LLATKKTNIWKHRLKWILCI--KKVNDEERNALDSAANIFASLCEESNLVFSDFEAGIQLLCK-QDANAYDFTEYSLKINKSKSGVTNSSSFNQKMYTIINHFINNEENYVNMSNFKKLANENLIETDSKVNNSEVNLADKISTGNKSDLKSDWRDIRFIYYFSTMAKASYGWPIHLLFHDENKSRLKFCMGLCCKHSTSCFCNKCICCDCEKSNSNDQKLIYVKE-RFCSCHEKAILNETHLEKENLVCVSFYNKTFHSPFFIAISHEIEVIILSIRGTFSLKDILTDMDANLIEV-FEDGFSGYLAHSGMLNAAKNIKRKIQEENIIHNILNDN-----KNYRLIITGHSLGAGIAIILSCFYYHEFPNLKCYAFSPPLGTVNIKTADYCKDFVLSIVNGVEWVPRISL 1813
            MP +++F R+W +A+DDFV      F++R    II+      FH      ++I    +   Q   + + G L       I+ L++  MS +G +M+   R+ IP  +Y++L +S    I+ +V  AI  ++  +  + C   ++I I   ++++         +II C T+D  GR          G +V+         +A K  + WK   ++  C    +   +E   +       A   ++ ++V SDF AG+ LL + Q    +   E +L+  +    V   ++  +  + +I H    +E ++    +K++A                                         YF   A A+YGWPI++                   H  S  C +C C  C  ++S   K++ V E   C CH  A+     L   +++ VS++N  + SPF++A+ H  + +++ IRGT SL+DILTD       +  E G   +  H GM+  A  I+RK++ + I+H   N +     ++YRL++ GHSLGAG A IL+      +P+L CYA+SPP   +++  A Y +DFV+SIV G + VPR+SL
Sbjct:    1 MPGIVVFRRRWMVASDDFVIPFAIAFVVRGIWLIILGVSMILFHGFQHQHHLITKEENVCEQTIDYFIGGYLALLFATEIMELSVAWMSGKGSLMDTEPRNFIPALLYVRLMLS----IIEVVWLAIGVKWIFLDTQVCPLTDEIYIARAVVVFNWMFLFIVTVIILC-TFDTAGRTWFAMQRARDGGHVLDAPCKYRTRIAKKYESRWKGCFRYAFCCIGAEGQGDENTRVSPLCRTLAEFFQDLDVVPSDFAAGLVLLRRHQKCEEHQELERALQERR----VQEMTTVRENFFLLIFH---TQEEFLL---YKEIA-----------------------------------------YFMNHALAAYGWPIYMK-----------------NHVLSFVCTRC-CYGCRNNDSRLHKIVQVDEDNCCRCHFSALKRIAGLHNTDVIYVSYHNSLYKSPFYVALDHHKKAVVVVIRGTLSLQDILTDFTVEPERIPAEGGDPSWFGHKGMVRCAVYIQRKLENDGILHTAFNADPDCGTRSYRLVLLGHSLGAGTAAILAFLLRPRYPDLFCYAYSPPGANLSLSAAKYARDFVISIVIGKDLVPRLSL 536          
BLAST of Sn1-specific diacylglycerol lipase beta vs. Ensembl Nematostella
Match: EDO44910 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RUI5])

HSP 1 Score: 152.14 bits (383), Expect = 4.541e-39
Identity = 93/323 (28.79%), Postives = 170/323 (52.63%), Query Frame = 2
Query:  908 FINNEENYVN-MSNFKKLANENLIETDSKVNNSEVNLADKISTGNKSDLKSDWRDIR----FIYYFSTMAKASYGWPIHLLFHDENKSRLKFCMGLC--CKHSTSCFCNKCICCDCEKSNSNDQKLIYVKERFCSCHEKAILNETHLEKENLVCVSFYNKTFHSPFFIAISHEIEVIILSIRGTFSLKDILTDMDANLIEVFEDGFSGYLAHSGMLNAAKNIKRKIQEENIIHNILNDN----KNYRLIITGHSLGAGIAIILSCFYYHEFPNLKCYAFSPPLGTVNIKTADYCKDFVLSIVNGVEWVPRISLQSIVVAKRRL 1843
            F  N++ Y+N +S   ++    L   D+  ++  V L   I    + +++S  ++ R       YF   +  SYGWP+++  +         C G C  C+ +++C         C  +NS       +K+  C C+  AI  +T L  E+L   S++NK +  PF++A+  + + +++SIRGT S+KD LTD+  +  ++  +G  G +AH GM  +A  IK ++ +  I+    ++     K+YRL++ GHSLGAG A +LS      +P+L+C+A+S P   +N       +D+++S++ G + VPR+ ++++   KR L
Sbjct:  203 FAGNDQQYMNAISQIAEMFASELHGVDAVPSDVAVGL---ILLQQQQEIESRGKEERSRIDAAAYFMRYSVGSYGWPMYVFMNP--------CCGPCSLCQKASAC---------CYPANSLAH---IIKDNCCMCNTAAIHLQTELRNEDLFYCSYHNKLYEIPFYVALDRDKKTVVVSIRGTLSMKDTLTDLTGHGEDIHIEGLDGGMAHKGMYLSATYIKGQLIDHGILQEAFDEAAKTVKSYRLVVVGHSLGAGTASVLSVLLKPMYPDLQCFAYSNP-SCLNESACTRTEDYIMSVIVGKDVVPRMGVKNLNTLKRDL 501          

HSP 2 Score: 63.5438 bits (153), Expect = 2.095e-10
Identity = 70/286 (24.48%), Postives = 121/286 (42.31%), Query Frame = 2
Query:  104 MPPLIMFGRKWNIATDDFVYLSFWEFLIRL--FIIVAYVYFNFHENMINNCNSKLYQICHFLLSGSLTCNXXXXXXXXX-XXXQGLIMEESKRSKIPFCIYIKLFMSFYEAILSIVAFAINYRYSLIPNKFCEQILIPNKIILYCIGPFLTAYLIIFCITYDQLGR-HGSNVIL-------------LATKKTNIWKHRLKWILCIKKVNDEE-RNALDSAANIFASLCEESNLVFSDFEAGIQLLCKQDANAYDFTEYSLKINKSKSGVTNS-SSFNQKMYTIIN 904
            MP L+ F R+W I +DD V+      + R   FI+V  +Y  +H+++   C+S++     F+    + C ++     +I  S QG IM    R +IP  +YI+L +   E  LSI+     +  S          +    ++ + I   + A   +F   +D LG  H  +  +             L      +W+ R + + C    ND++  NA+   A +FAS     + V SD   G+ LL +Q        E   +I+ +   +  S  S+   MY  +N
Sbjct:    1 MPALVAFNRRWQIGSDDLVFPFTAGLMARFAWFIVVCSIYGVYHKDL-KECHSQIALQTFFIGVIIVACLVMGNEVAIIWTSMQGTIMNTRPRQRIPILLYIQLALYVPEIALSILGIYWAFHSSTGCELGVVVPVQITVVLQWLILLSVFAAAAMF---FDPLGALHKQDRSIEQSSKFQCQTFSKLVAALVTLWERRCRLLCCCFAGNDQQYMNAISQIAEMFASELHGVDAVPSDVAVGLILLQQQQEIESRGKEERSRIDAAAYFMRYSVGSYGWPMYVFMN 282          
BLAST of Sn1-specific diacylglycerol lipase beta vs. Ensembl Nematostella
Match: EDO44911 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RUI6])

HSP 1 Score: 85.5001 bits (210), Expect = 1.353e-17
Identity = 50/164 (30.49%), Postives = 85/164 (51.83%), Query Frame = 2
Query: 1034 GNKSDLKSDWRD-IRFIYYFSTMAKASYGWPIHLLFHDENKSRLKFCMGLCCKHSTSCFCNKCICCDCEKSNSNDQKLIYVKERFCSCHEKAILNETHLEKENLVCVSFYNKTFHSPFFIAISHEIEVIILSIRGTFSLKDILTDMDANLIEVFEDGFSGYLAH 1522
            G+   ++S+ R  I    YF   +  SYGWP+++             M  CC   + C   +C  C C  +NS       +K+  C C+  AI  +T L  E+L+  S++NK +  PF++A+  + + +++SIRGT S+KD LTD+  +  ++  +G  G +AH
Sbjct:  247 GSAPGMESEERSRIDAAAYFMRYSVGSYGWPMYVF------------MNPCCGPCSLCQKARCSAC-CYPANSLAH---IIKDNCCMCNTAAIHLQTELRNEDLLYCSYHNKLYEIPFYVALDRDKKTVVVSIRGTLSMKDTLTDLTGHGEDIHIEGLDGGMAH 394          
BLAST of Sn1-specific diacylglycerol lipase beta vs. Ensembl Medaka
Match: dagla (diacylglycerol lipase alpha [Source:NCBI gene;Acc:101165873])

HSP 1 Score: 207.608 bits (527), Expect = 2.072e-55
Identity = 192/745 (25.77%), Postives = 332/745 (44.56%), Query Frame = 2
Query:  104 MPPLIMFGRKWNIATDDFVYLSFWEFLIR--LFIIVAYVYFNFHENMINNCNSKLYQICHFLLSGSLTCNXXXXXXXXXXXXQGLIMEESKRSKIPFCIYIKLFMSFYEAILSIVAFAINYRYSLIPNKFCEQILIPNKI--ILYCIGPFLTAYLIIFCITYDQLGRHGSNVILLATKK------------------TNIWKHRLKWILCIKKVNDEERNALDSAANIFASLCEESNLVFSDFEAGIQLLCKQDANAYDFTEYSLKINKSKSGVTNSSSFNQKMYTIINHFINNEENYVNMSNFKKLANENLIETDSKVNNSEVNLADKISTGNKSDLKSDWRDIRFIYYFSTMAKASYGWPIHLLFHDENKSRLKFCMGLCCKHSTSCFCNKCICCDCEKSNSNDQKLIYVKE-RFCSCHEKAILN---ETHLEKENLVCVSFYNKTFHSPFFIAISHEIEVIILSIRGTFSLKDILTDM--DANLIEVFEDGFSGYLAHSGMLNAAKNIKRKIQEENII-----HNILNDNKNYRLIITGHSLGAGIAIILSCFYYHEFPNLKCYAFSPPLGTVNIKTADYCKDFVLSIVNGVEWVPRISLQSIVVAKRRLIECLE--NYPHXXXXXXXXXXXXXXXXXXXXXXXRWNILNSFERDEKYWEN-------IEINTKL--PGQIIHITNIKKLNDPNKSDLINSNRSFYTPIITSSDQFKTVVFHPKMFKDHFPHQMVKRLFNIL 2206
            MP ++MF R+W++ +DD V  + + FLI     ++++ V F         C+  L       L   ++C LI   AIM +S +G I+    R  + + IYI+L +   E   ++V  A   +Y     + C  +   N    I+ C    + +  I    T+D  GR  + V L AT++                   + W  RLK+ +C  +  D + +A    A++FA    + ++V SD  AG+ LL ++               ++K       + N  +  +    +     Y+++ N                 ++E+N+               ++D+    Y+   A A+YGWP++L+       R   C GLC   ++SC C          S S   + + V+E   C C+  AI     +  L++ ++V  S ++  + +PFF+A+ H    +++SIRGT S KD LTD+  D+  + V E+    +L H GM+ +A+ IK+K+++E I+      ++     +Y L+I GHSLGAG A ILS     ++PNL CY++SPP G ++    +Y K+FV ++V G + VPR+ L  +   +R L+E L+  N P  +      K I                 NS     + W +       +  +T L  PG+IIH+ +    N P ++         Y+ +   +  F  V+  P M  +H PH ++K L  +L
Sbjct:    1 MPGMVMFRRRWSVGSDDLVLPALFLFLIHCIWLVVLSVVLFGLPYGSEQPCSLTLVDHGRGYLGILVSC-LICESAIMWLSMRGSILHTQPREAVQYVIYIRLTILLVELAYAVVGIAWLVQY----YQPCSDVTAKNLALGIVSCNWLVILSVCITLMCTFDPTGR--TFVKLKATRRRQRNLTTYTLRHRLEEGQASSWSRRLKFFMCCTRAQDTQSDAYSEVASLFAEFFRDLDIVPSDIIAGLVLLRQR--------------QRAKRSAILDQANNDILAFLSGIPVTRNTKYLDLKN-----------------STEMNM---------------YKDV---CYYMLFAMAAYGWPMYLM-------RNPAC-GLC-HLASSCPCTSV-------SGSRLSQSVTVEEDNCCGCNVLAIRRHFLDKDLKQVHIVYTSCHDAVYETPFFVAVDHVKRKVVISIRGTLSPKDALTDLTGDSERLPV-EEQHGTWLGHKGMVYSAEYIKKKLEQEMILSQAFGRDLSKGTMHYELVIVGHSLGAGTAAILSFLLRPQYPNLHCYSYSPPGGLLSEDAMEYSKEFVTAVVLGKDLVPRLGLSQLEGFRRHLLEVLQKSNKPKWRIIAGGTKCIPKSELPVEDDPQPAPPPNS-----RLWLHPSDLSIALSASTPLYPPGKIIHVVH----NHPPET-CCGQEEPTYSALWGDNKAFNEVIISPAMLNEHMPHMVMKGLNKVL 662          
BLAST of Sn1-specific diacylglycerol lipase beta vs. Ensembl Medaka
Match: dagla (diacylglycerol lipase alpha [Source:NCBI gene;Acc:101165873])

HSP 1 Score: 207.608 bits (527), Expect = 3.529e-55
Identity = 192/745 (25.77%), Postives = 332/745 (44.56%), Query Frame = 2
Query:  104 MPPLIMFGRKWNIATDDFVYLSFWEFLIR--LFIIVAYVYFNFHENMINNCNSKLYQICHFLLSGSLTCNXXXXXXXXXXXXQGLIMEESKRSKIPFCIYIKLFMSFYEAILSIVAFAINYRYSLIPNKFCEQILIPNKI--ILYCIGPFLTAYLIIFCITYDQLGRHGSNVILLATKK------------------TNIWKHRLKWILCIKKVNDEERNALDSAANIFASLCEESNLVFSDFEAGIQLLCKQDANAYDFTEYSLKINKSKSGVTNSSSFNQKMYTIINHFINNEENYVNMSNFKKLANENLIETDSKVNNSEVNLADKISTGNKSDLKSDWRDIRFIYYFSTMAKASYGWPIHLLFHDENKSRLKFCMGLCCKHSTSCFCNKCICCDCEKSNSNDQKLIYVKE-RFCSCHEKAILN---ETHLEKENLVCVSFYNKTFHSPFFIAISHEIEVIILSIRGTFSLKDILTDM--DANLIEVFEDGFSGYLAHSGMLNAAKNIKRKIQEENII-----HNILNDNKNYRLIITGHSLGAGIAIILSCFYYHEFPNLKCYAFSPPLGTVNIKTADYCKDFVLSIVNGVEWVPRISLQSIVVAKRRLIECLE--NYPHXXXXXXXXXXXXXXXXXXXXXXXRWNILNSFERDEKYWEN-------IEINTKL--PGQIIHITNIKKLNDPNKSDLINSNRSFYTPIITSSDQFKTVVFHPKMFKDHFPHQMVKRLFNIL 2206
            MP ++MF R+W++ +DD V  + + FLI     ++++ V F         C+  L       L   ++C LI   AIM +S +G I+    R  + + IYI+L +   E   ++V  A   +Y     + C  +   N    I+ C    + +  I    T+D  GR  + V L AT++                   + W  RLK+ +C  +  D + +A    A++FA    + ++V SD  AG+ LL ++               ++K       + N  +  +    +     Y+++ N                 ++E+N+               ++D+    Y+   A A+YGWP++L+       R   C GLC   ++SC C          S S   + + V+E   C C+  AI     +  L++ ++V  S ++  + +PFF+A+ H    +++SIRGT S KD LTD+  D+  + V E+    +L H GM+ +A+ IK+K+++E I+      ++     +Y L+I GHSLGAG A ILS     ++PNL CY++SPP G ++    +Y K+FV ++V G + VPR+ L  +   +R L+E L+  N P  +      K I                 NS     + W +       +  +T L  PG+IIH+ +    N P ++         Y+ +   +  F  V+  P M  +H PH ++K L  +L
Sbjct:    1 MPGMVMFRRRWSVGSDDLVLPALFLFLIHCIWLVVLSVVLFGLPYGSEQPCSLTLVDHGRGYLGILVSC-LICESAIMWLSMRGSILHTQPREAVQYVIYIRLTILLVELAYAVVGIAWLVQY----YQPCSDVTAKNLALGIVSCNWLVILSVCITLMCTFDPTGR--TFVKLKATRRRQRNLTTYTLRHRLEEGQASSWSRRLKFFMCCTRAQDTQSDAYSEVASLFAEFFRDLDIVPSDIIAGLVLLRQR--------------QRAKRSAILDQANNDILAFLSGIPVTRNTKYLDLKN-----------------STEMNM---------------YKDV---CYYMLFAMAAYGWPMYLM-------RNPAC-GLC-HLASSCPCTSV-------SGSRLSQSVTVEEDNCCGCNVLAIRRHFLDKDLKQVHIVYTSCHDAVYETPFFVAVDHVKRKVVISIRGTLSPKDALTDLTGDSERLPV-EEQHGTWLGHKGMVYSAEYIKKKLEQEMILSQAFGRDLSKGTMHYELVIVGHSLGAGTAAILSFLLRPQYPNLHCYSYSPPGGLLSEDAMEYSKEFVTAVVLGKDLVPRLGLSQLEGFRRHLLEVLQKSNKPKWRIIAGGTKCIPKSELPVEDDPQPAPPPNS-----RLWLHPSDLSIALSASTPLYPPGKIIHVVH----NHPPET-CCGQEEPTYSALWGDNKAFNEVIISPAMLNEHMPHMVMKGLNKVL 662          
BLAST of Sn1-specific diacylglycerol lipase beta vs. Ensembl Medaka
Match: daglb (diacylglycerol lipase beta [Source:NCBI gene;Acc:101154852])

HSP 1 Score: 178.333 bits (451), Expect = 1.149e-46
Identity = 95/258 (36.82%), Postives = 149/258 (57.75%), Query Frame = 2
Query: 1094 TMAKASYGWPIHLLFHDENKSRLKFCMGLCCKHSTSCFCNKC-ICCDCEKSNSNDQKLIYVKERFCSCHEKAILNETHLEKENLVCVSFYNKTFHSPFFIAISHEIEVIILSIRGTFSLKDILTDMDANLIEVFEDGFSGY-LAHSGMLNAAKNIKRKIQEENIIHNILNDNKNYRLIITGHSLGAGIAIILSCFYYHEFPNLKCYAFSPPLGTVNIKTADYCKDFVLSIVNGVEWVPRISLQSIVVAKRRLIECLEN 1861
            T A A+YGWP+++                     ++ F   C +  DC +S++ D  +  V      CH  +IL  T L+  + + VSF+N+ +  PFF+A+ H+ E +++++RGT SLKD+LTD+ A   ++  +G SG   AH GM  AA  I RK+  + I++   +    Y+L+ITGHSLGAG A +L+      FP L+CYAFSPP G ++   ADY K FV+SIV G + VPR+S+ ++   K+R+++ + N
Sbjct:  287 TFAAAAYGWPLYVY--------------------SNPFSAPCKLSGDCCRSHAGDYDI--VGGDHLGCHFASILESTGLQYRDFIHVSFHNQIYEIPFFVALDHKREAVVVAVRGTLSLKDVLTDLSAECEQLPVEGVSGACYAHKGMCQAAGYIYRKLVNDGILNQAFSIAPEYKLVITGHSLGAGTASVLAILLRSSFPTLQCYAFSPPGGLLSKALADYSKGFVVSIVLGKDLVPRLSIPNMEDLKKRILKMVSN 522          
BLAST of Sn1-specific diacylglycerol lipase beta vs. Planmine SMEST
Match: SMESG000046500.1 (SMESG000046500.1)

HSP 1 Score: 755.362 bits (1949), Expect = 0.000e+0
Identity = 380/422 (90.05%), Postives = 382/422 (90.52%), Query Frame = 2
Query:  104 MPPLIMFGRKWNIATDDFVYLSFWEFLIRLFIIVAYVYFNFHENMINNCNSKLYQICHFLLSGSLTCNXXXXXXXXXXXXQGLIMEESKRSKIPFCIYIKLFMSFYEAILSIVAFAINYRYSLIPNKFCEQILIPNKIILYCIGPFLTAYLIIFCITYDQLGRHGSNVILLATKKTNIWKHRLKWILCIKKVNDEERNALDSAANIFASLCEESNLVFSDFEAGIQLLCKQDANAYDFTEYSLKINKSKSGVTNSSSFNQKMYTIINHFINNEENYVNMSNFKKLANENLIETDSKVNNSEVNLADKISTGNKSDLKSDWRDIRFIYYFSTMAKASYGWPIHLLFHDENKSRLKFCMGLCCKHSTSCFCNKCICCDCEKSNSNDQKLIYVKERFCSCHEKAILNETHLEKENLVCVSFYNKT 1369
            MPPLIMFGRKWNIATDDFVYLSFWEFLIRLFIIVAYVYFNFHENMINNCNSKLYQ   +                    AQGLIMEESKRSKIPFCIYIKLFMSFYEAILSIVAFAINYRYSLIPNKFCEQILIPNKIILYCIGPFLTAYLIIFCITYDQLGRHGSNVILLATKKTNIWKHRLKWILCIKKVNDEERNALDSAANIFASLCEESNLVFSDFEAGIQLLCKQDANAYDFTEYSLKINKSKSGVTNSSSFNQKMYTIINHFINNEENYVNMSNFK                +EVNLADKISTGNKSDLKSDWRDIRFIYYFSTMAKASYGWPIHLLFHDENKSRLKFCMGLCCKHSTSCFCNKCICCDCEKSNSNDQKLIYVKERFCSCHEKAILNETHLEKENLVCVSFYNK 
Sbjct:    1 MPPLIMFGRKWNIATDDFVYLSFWEFLIRLFIIVAYVYFNFHENMINNCNSKLYQWKSYF-------------------AQGLIMEESKRSKIPFCIYIKLFMSFYEAILSIVAFAINYRYSLIPNKFCEQILIPNKIILYCIGPFLTAYLIIFCITYDQLGRHGSNVILLATKKTNIWKHRLKWILCIKKVNDEERNALDSAANIFASLCEESNLVFSDFEAGIQLLCKQDANAYDFTEYSLKINKSKSGVTNSSSFNQKMYTIINHFINNEENYVNMSNFK----------------NEVNLADKISTGNKSDLKSDWRDIRFIYYFSTMAKASYGWPIHLLFHDENKSRLKFCMGLCCKHSTSCFCNKCICCDCEKSNSNDQKLIYVKERFCSCHEKAILNETHLEKENLVCVSFYNKV 387          
BLAST of Sn1-specific diacylglycerol lipase beta vs. Planmine SMEST
Match: SMESG000046500.1 (SMESG000046500.1)

HSP 1 Score: 753.436 bits (1944), Expect = 0.000e+0
Identity = 380/421 (90.26%), Postives = 382/421 (90.74%), Query Frame = 2
Query:  104 MPPLIMFGRKWNIATDDFVYLSFWEFLIRLFIIVAYVYFNFHENMINNCNSKLYQICHFLLSGSLTCNXXXXXXXXXXXXQGLIMEESKRSKIPFCIYIKLFMSFYEAILSIVAFAINYRYSLIPNKFCEQILIPNKIILYCIGPFLTAYLIIFCITYDQLGRHGSNVILLATKKTNIWKHRLKWILCIKKVNDEERNALDSAANIFASLCEESNLVFSDFEAGIQLLCKQDANAYDFTEYSLKINKSKSGVTNSSSFNQKMYTIINHFINNEENYVNMSNFKKLANENLIETDSKVNNSEVNLADKISTGNKSDLKSDWRDIRFIYYFSTMAKASYGWPIHLLFHDENKSRLKFCMGLCCKHSTSCFCNKCICCDCEKSNSNDQKLIYVKERFCSCHEKAILNETHLEKENLVCVSFYNK 1366
            MPPLIMFGRKWNIATDDFVYLSFWEFLIRLFIIVAYVYFNFHENMINNCNSKLYQ   +                    AQGLIMEESKRSKIPFCIYIKLFMSFYEAILSIVAFAINYRYSLIPNKFCEQILIPNKIILYCIGPFLTAYLIIFCITYDQLGRHGSNVILLATKKTNIWKHRLKWILCIKKVNDEERNALDSAANIFASLCEESNLVFSDFEAGIQLLCKQDANAYDFTEYSLKINKSKSGVTNSSSFNQKMYTIINHFINNEENYVNMSNFK                +EVNLADKISTGNKSDLKSDWRDIRFIYYFSTMAKASYGWPIHLLFHDENKSRLKFCMGLCCKHSTSCFCNKCICCDCEKSNSNDQKLIYVKERFCSCHEKAILNETHLEKENLVCVSFYNK
Sbjct:    1 MPPLIMFGRKWNIATDDFVYLSFWEFLIRLFIIVAYVYFNFHENMINNCNSKLYQWKSYF-------------------AQGLIMEESKRSKIPFCIYIKLFMSFYEAILSIVAFAINYRYSLIPNKFCEQILIPNKIILYCIGPFLTAYLIIFCITYDQLGRHGSNVILLATKKTNIWKHRLKWILCIKKVNDEERNALDSAANIFASLCEESNLVFSDFEAGIQLLCKQDANAYDFTEYSLKINKSKSGVTNSSSFNQKMYTIINHFINNEENYVNMSNFK----------------NEVNLADKISTGNKSDLKSDWRDIRFIYYFSTMAKASYGWPIHLLFHDENKSRLKFCMGLCCKHSTSCFCNKCICCDCEKSNSNDQKLIYVKERFCSCHEKAILNETHLEKENLVCVSFYNK 386          
BLAST of Sn1-specific diacylglycerol lipase beta vs. Planmine SMEST
Match: SMESG000046500.1 (SMESG000046500.1)

HSP 1 Score: 719.924 bits (1857), Expect = 0.000e+0
Identity = 365/417 (87.53%), Postives = 367/417 (88.01%), Query Frame = 2
Query:  119 MFGRKWNIATDDFVYLSFWEFLIRLFIIVAYVYFNFHENMINNCNSKLYQICHFLLSGSLTCNXXXXXXXXXXXXQGLIMEESKRSKIPFCIYIKLFMSFYEAILSIVAFAINYRYSLIPNKFCEQILIPNKIILYCIGPFLTAYLIIFCITYDQLGRHGSNVILLATKKTNIWKHRLKWILCIKKVNDEERNALDSAANIFASLCEESNLVFSDFEAGIQLLCKQDANAYDFTEYSLKINKSKSGVTNSSSFNQKMYTIINHFINNEENYVNMSNFKKLANENLIETDSKVNNSEVNLADKISTGNKSDLKSDWRDIRFIYYFSTMAKASYGWPIHLLFHDENKSRLKFCMGLCCKHSTSCFCNKCICCDCEKSNSNDQKLIYVKERFCSCHEKAILNETHLEKENLVCVSFYNKT 1369
            MFGRKWNIATDDFVYLSFWEFLIRLFIIVAYVYFNFHENMINNCNSKLYQ   +L                              SKIPFCIYIKLFMSFYEAILSIVAFAINYRYSLIPNKFCEQILIPNKIILYCIGPFLTAYLIIFCITYDQLGRHGSNVILLATKKTNIWKHRLKWILCIKKVNDEERNALDSAANIFASLCEESNLVFSDFEAGIQLLCKQDANAYDFTEYSLKINKSKSGVTNSSSFNQKMYTIINHFINNEENYVNMSNFK                +EVNLADKISTGNKSDLKSDWRDIRFIYYFSTMAKASYGWPIHLLFHDENKSRLKFCMGLCCKHSTSCFCNKCICCDCEKSNSNDQKLIYVKERFCSCHEKAILNETHLEKENLVCVSFYNK 
Sbjct:    1 MFGRKWNIATDDFVYLSFWEFLIRLFIIVAYVYFNFHENMINNCNSKLYQWKSYL------------------------------SKIPFCIYIKLFMSFYEAILSIVAFAINYRYSLIPNKFCEQILIPNKIILYCIGPFLTAYLIIFCITYDQLGRHGSNVILLATKKTNIWKHRLKWILCIKKVNDEERNALDSAANIFASLCEESNLVFSDFEAGIQLLCKQDANAYDFTEYSLKINKSKSGVTNSSSFNQKMYTIINHFINNEENYVNMSNFK----------------NEVNLADKISTGNKSDLKSDWRDIRFIYYFSTMAKASYGWPIHLLFHDENKSRLKFCMGLCCKHSTSCFCNKCICCDCEKSNSNDQKLIYVKERFCSCHEKAILNETHLEKENLVCVSFYNKV 371          
BLAST of Sn1-specific diacylglycerol lipase beta vs. Planmine SMEST
Match: SMESG000046500.1 (SMESG000046500.1)

HSP 1 Score: 696.041 bits (1795), Expect = 0.000e+0
Identity = 355/421 (84.32%), Postives = 357/421 (84.80%), Query Frame = 2
Query:  104 MPPLIMFGRKWNIATDDFVYLSFWEFLIRLFIIVAYVYFNFHENMINNCNSKLYQICHFLLSGSLTCNXXXXXXXXXXXXQGLIMEESKRSKIPFCIYIKLFMSFYEAILSIVAFAINYRYSLIPNKFCEQILIPNKIILYCIGPFLTAYLIIFCITYDQLGRHGSNVILLATKKTNIWKHRLKWILCIKKVNDEERNALDSAANIFASLCEESNLVFSDFEAGIQLLCKQDANAYDFTEYSLKINKSKSGVTNSSSFNQKMYTIINHFINNEENYVNMSNFKKLANENLIETDSKVNNSEVNLADKISTGNKSDLKSDWRDIRFIYYFSTMAKASYGWPIHLLFHDENKSRLKFCMGLCCKHSTSCFCNKCICCDCEKSNSNDQKLIYVKERFCSCHEKAILNETHLEKENLVCVSFYNK 1366
            MPPLIMFGRKWNIATDDFVYLSFWEFLIRLFIIVAYVYFNFHENMINNCNSKLYQ   +                                             FYEAILSIVAFAINYRYSLIPNKFCEQILIPNKIILYCIGPFLTAYLIIFCITYDQLGRHGSNVILLATKKTNIWKHRLKWILCIKKVNDEERNALDSAANIFASLCEESNLVFSDFEAGIQLLCKQDANAYDFTEYSLKINKSKSGVTNSSSFNQKMYTIINHFINNEENYVNMSNFK                +EVNLADKISTGNKSDLKSDWRDIRFIYYFSTMAKASYGWPIHLLFHDENKSRLKFCMGLCCKHSTSCFCNKCICCDCEKSNSNDQKLIYVKERFCSCHEKAILNETHLEKENLVCVSFYNK
Sbjct:    1 MPPLIMFGRKWNIATDDFVYLSFWEFLIRLFIIVAYVYFNFHENMINNCNSKLYQWKSYF--------------------------------------------FYEAILSIVAFAINYRYSLIPNKFCEQILIPNKIILYCIGPFLTAYLIIFCITYDQLGRHGSNVILLATKKTNIWKHRLKWILCIKKVNDEERNALDSAANIFASLCEESNLVFSDFEAGIQLLCKQDANAYDFTEYSLKINKSKSGVTNSSSFNQKMYTIINHFINNEENYVNMSNFK----------------NEVNLADKISTGNKSDLKSDWRDIRFIYYFSTMAKASYGWPIHLLFHDENKSRLKFCMGLCCKHSTSCFCNKCICCDCEKSNSNDQKLIYVKERFCSCHEKAILNETHLEKENLVCVSFYNK 361          
BLAST of Sn1-specific diacylglycerol lipase beta vs. Planmine SMEST
Match: SMESG000046500.1 (SMESG000046500.1)

HSP 1 Score: 635.565 bits (1638), Expect = 0.000e+0
Identity = 320/361 (88.64%), Postives = 322/361 (89.20%), Query Frame = 2
Query:  104 MPPLIMFGRKWNIATDDFVYLSFWEFLIRLFIIVAYVYFNFHENMINNCNSKLYQICHFLLSGSLTCNXXXXXXXXXXXXQGLIMEESKRSKIPFCIYIKLFMSFYEAILSIVAFAINYRYSLIPNKFCEQILIPNKIILYCIGPFLTAYLIIFCITYDQLGRHGSNVILLATKKTNIWKHRLKWILCIKKVNDEERNALDSAANIFASLCEESNLVFSDFEAGIQLLCKQDANAYDFTEYSLKINKSKSGVTNSSSFNQKMYTIINHFINNEENYVNMSNFKKLANENLIETDSKVNNSEVNLADKISTGNKSDLKSDWRDIRFIYYFSTMAKASYGWPIHLLFHDENKSRLKFCMGLCC 1186
            MPPLIMFGRKWNIATDDFVYLSFWEFLIRLFIIVAYVYFNFHENMINNCNSKLYQ   +                    AQGLIMEESKRSKIPFCIYIKLFMSFYEAILSIVAFAINYRYSLIPNKFCEQILIPNKIILYCIGPFLTAYLIIFCITYDQLGRHGSNVILLATKKTNIWKHRLKWILCIKKVNDEERNALDSAANIFASLCEESNLVFSDFEAGIQLLCKQDANAYDFTEYSLKINKSKSGVTNSSSFNQKMYTIINHFINNEENYVNMSNFK                +EVNLADKISTGNKSDLKSDWRDIRFIYYFSTMAKASYGWPIHLLFHDENKSRLKFCMGLCC
Sbjct:    1 MPPLIMFGRKWNIATDDFVYLSFWEFLIRLFIIVAYVYFNFHENMINNCNSKLYQWKSYF-------------------AQGLIMEESKRSKIPFCIYIKLFMSFYEAILSIVAFAINYRYSLIPNKFCEQILIPNKIILYCIGPFLTAYLIIFCITYDQLGRHGSNVILLATKKTNIWKHRLKWILCIKKVNDEERNALDSAANIFASLCEESNLVFSDFEAGIQLLCKQDANAYDFTEYSLKINKSKSGVTNSSSFNQKMYTIINHFINNEENYVNMSNFK----------------NEVNLADKISTGNKSDLKSDWRDIRFIYYFSTMAKASYGWPIHLLFHDENKSRLKFCMGLCC 326          
The following BLAST results are available for this feature:
BLAST of Sn1-specific diacylglycerol lipase beta vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 4
Match NameE-valueIdentityDescription
DAGLA1.876e-5323.50diacylglycerol lipase alpha [Source:HGNC Symbol;Ac... [more]
DAGLB1.078e-5024.71diacylglycerol lipase beta [Source:HGNC Symbol;Acc... [more]
DAGLB3.202e-3924.10diacylglycerol lipase beta [Source:HGNC Symbol;Acc... [more]
DAGLB2.826e-2932.86diacylglycerol lipase beta [Source:HGNC Symbol;Acc... [more]
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BLAST of Sn1-specific diacylglycerol lipase beta vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 3
Match NameE-valueIdentityDescription
dagl-26.238e-3127.93DiAcylGlycerol Lipase homolog [Source:UniProtKB/T... [more]
dagl-22.347e-3028.32DiAcylGlycerol Lipase homolog [Source:UniProtKB/T... [more]
dagl-22.364e-3028.32DiAcylGlycerol Lipase homolog [Source:UniProtKB/T... [more]
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BLAST of Sn1-specific diacylglycerol lipase beta vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
inaE1.122e-3926.13gene:FBgn0261244 transcript:FBtr0073826[more]
inaE1.422e-3926.13gene:FBgn0261244 transcript:FBtr0073825[more]
inaE1.495e-3926.13gene:FBgn0261244 transcript:FBtr0290303[more]
inaE1.505e-3926.13gene:FBgn0261244 transcript:FBtr0340291[more]
inaE1.617e-3926.13gene:FBgn0261244 transcript:FBtr0290302[more]
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BLAST of Sn1-specific diacylglycerol lipase beta vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
dagla7.582e-5625.17diacylglycerol lipase, alpha [Source:ZFIN;Acc:ZDB-... [more]
dagla7.582e-5625.17diacylglycerol lipase, alpha [Source:ZFIN;Acc:ZDB-... [more]
BX248389.17.459e-5524.83diacylglycerol lipase, alpha [Source:NCBI gene;Acc... [more]
BX248389.13.354e-5424.36diacylglycerol lipase, alpha [Source:NCBI gene;Acc... [more]
BX248389.13.675e-5424.36diacylglycerol lipase, alpha [Source:NCBI gene;Acc... [more]
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BLAST of Sn1-specific diacylglycerol lipase beta vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
cxxc47.193e-5223.61CXXC finger protein 4 [Source:Xenbase;Acc:XB-GENE-... [more]
cxxc43.161e-5123.50CXXC finger protein 4 [Source:Xenbase;Acc:XB-GENE-... [more]
cxxc41.708e-4923.51CXXC finger protein 4 [Source:Xenbase;Acc:XB-GENE-... [more]
cxxc41.755e-4922.99CXXC finger protein 4 [Source:Xenbase;Acc:XB-GENE-... [more]
polr3c4.110e-4926.14polymerase (RNA) III (DNA directed) polypeptide C ... [more]
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BLAST of Sn1-specific diacylglycerol lipase beta vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 2
Match NameE-valueIdentityDescription
Dagla2.429e-4823.83diacylglycerol lipase, alpha [Source:MGI Symbol;Ac... [more]
Daglb1.147e-4424.38diacylglycerol lipase, beta [Source:MGI Symbol;Acc... [more]
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BLAST of Sn1-specific diacylglycerol lipase beta vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q9Y4D2|DGLA_HUMAN9.007e-5323.50Sn1-specific diacylglycerol lipase alpha OS=Homo s... [more]
sp|Q8NCG7|DGLB_HUMAN5.178e-5024.71Sn1-specific diacylglycerol lipase beta OS=Homo sa... [more]
sp|Q6WQJ1|DGLA_MOUSE1.699e-4723.83Sn1-specific diacylglycerol lipase alpha OS=Mus mu... [more]
sp|Q5YLM1|DGLA_RAT1.927e-4724.23Sn1-specific diacylglycerol lipase alpha OS=Rattus... [more]
sp|Q91WC9|DGLB_MOUSE8.024e-4424.38Sn1-specific diacylglycerol lipase beta OS=Mus mus... [more]
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BLAST of Sn1-specific diacylglycerol lipase beta vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A3R7PXD31.164e-5827.99Lipase_3 domain-containing protein OS=Penaeus vann... [more]
A0A0B8RUR97.029e-5824.80Diacylglycerol lipase, beta OS=Boiga irregularis O... [more]
A0A210PTB03.573e-5725.32Sn1-specific diacylglycerol lipase beta OS=Mizuhop... [more]
V9KEV64.243e-5725.36Sn1-specific diacylglycerol lipase beta OS=Callorh... [more]
A0A2T7PVV31.221e-5625.00Lipase_3 domain-containing protein OS=Pomacea cana... [more]
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BLAST of Sn1-specific diacylglycerol lipase beta vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 3
Match NameE-valueIdentityDescription
dagla1.468e-5124.16diacylglycerol lipase alpha [Source:NCBI gene;Acc:... [more]
dagla7.504e-5024.19diacylglycerol lipase alpha [Source:NCBI gene;Acc:... [more]
daglb1.786e-4227.92diacylglycerol lipase beta [Source:NCBI gene;Acc:1... [more]
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BLAST of Sn1-specific diacylglycerol lipase beta vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 2
Match NameE-valueIdentityDescription
daglb2.684e-4031.25diacylglycerol lipase, beta [Source:ZFIN;Acc:ZDB-G... [more]
ENSPMAT00000003941.13.464e-1743.24pep scaffold:Pmarinus_7.0:GL487229:269:2582:1 gene... [more]
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BLAST of Sn1-specific diacylglycerol lipase beta vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Sn1-specific diacylglycerol lipase beta vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 3
Match NameE-valueIdentityDescription
EDO437107.251e-5626.56Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO449104.541e-3928.79Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO449111.353e-1730.49Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Sn1-specific diacylglycerol lipase beta vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 3
Match NameE-valueIdentityDescription
dagla2.072e-5525.77diacylglycerol lipase alpha [Source:NCBI gene;Acc:... [more]
dagla3.529e-5525.77diacylglycerol lipase alpha [Source:NCBI gene;Acc:... [more]
daglb1.149e-4636.82diacylglycerol lipase beta [Source:NCBI gene;Acc:1... [more]
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BLAST of Sn1-specific diacylglycerol lipase beta vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000046500.10.000e+090.05SMESG000046500.1[more]
SMESG000046500.10.000e+090.26SMESG000046500.1[more]
SMESG000046500.10.000e+087.53SMESG000046500.1[more]
SMESG000046500.10.000e+084.32SMESG000046500.1[more]
SMESG000046500.10.000e+088.64SMESG000046500.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30023344 ID=SMED30023344|Name=Sn1-specific diacylglycerol lipase beta|organism=Schmidtea mediterranea sexual|type=transcript|length=2484bp
TCTATATAAATCTTATATGTTAGCTTTTAATAGTAAACGATTTTTGAGTA
TTTCAACACGTGGATCAGCTTGTCTAAAACAAAGATTTTTTCATTATTTA
TAAATGCCTCCTCTCATTATGTTTGGTCGTAAGTGGAATATAGCTACAGA
TGATTTTGTCTACCTTTCATTTTGGGAATTTTTAATTCGATTATTCATCA
TTGTTGCATATGTTTATTTTAATTTCCATGAAAATATGATAAATAACTGC
AATAGCAAACTATATCAAATATGTCATTTTTTATTAAGTGGAAGTCTTAC
TTGTAATTTGATAATAATTTTAGCAATCATGATTATGAGCGCTCAAGGAT
TAATAATGGAAGAATCCAAAAGATCAAAAATTCCTTTTTGCATATACATT
AAATTATTTATGAGTTTTTACGAAGCAATTTTGTCGATTGTTGCTTTTGC
GATAAATTATCGATATTCTTTGATTCCTAATAAATTTTGTGAGCAAATTC
TGATTCCAAACAAAATTATATTATACTGCATTGGACCATTCTTGACTGCA
TATCTCATAATTTTTTGTATTACTTATGATCAATTAGGAAGACATGGTAG
TAATGTTATTCTACTAGCAACAAAAAAAACAAATATATGGAAGCATCGTT
TGAAATGGATTTTATGTATAAAAAAAGTAAATGATGAAGAAAGGAATGCT
CTAGATTCTGCCGCAAATATTTTCGCTTCATTATGTGAGGAATCAAATCT
GGTTTTCAGTGACTTCGAAGCTGGTATTCAATTACTATGCAAACAAGATG
CAAATGCATATGATTTTACCGAGTATTCTTTGAAAATAAATAAATCTAAA
TCTGGTGTCACGAATTCGAGTAGTTTCAACCAAAAAATGTATACAATCAT
CAACCATTTTATAAACAATGAAGAAAATTATGTGAACATGTCAAATTTTA
AAAAGTTAGCCAATGAAAATCTAATAGAAACTGATTCAAAAGTAAATAAT
AGTGAAGTTAATTTAGCTGATAAAATTTCAACTGGAAATAAAAGTGATTT
AAAATCGGACTGGAGAGATATTCGTTTTATTTATTATTTTTCAACAATGG
CCAAAGCTTCATATGGCTGGCCTATTCATCTGTTATTTCACGATGAAAAT
AAATCAAGATTAAAGTTTTGCATGGGTCTGTGTTGTAAGCATTCAACTTC
GTGTTTCTGTAATAAATGCATATGTTGTGATTGTGAAAAATCGAATTCAA
ATGATCAAAAGTTAATTTATGTTAAAGAAAGGTTTTGTAGTTGCCATGAA
AAAGCCATTTTAAACGAAACACATTTAGAAAAGGAAAATCTTGTATGTGT
TTCATTTTATAATAAGACATTTCATTCTCCTTTCTTTATTGCAATATCTC
ATGAGATTGAAGTTATTATTTTATCTATTCGAGGAACGTTTTCATTGAAA
GATATTTTAACTGATATGGACGCGAATCTCATTGAAGTATTTGAAGACGG
ATTTTCTGGATATCTGGCACATTCTGGTATGTTAAATGCAGCTAAAAATA
TTAAAAGAAAAATTCAAGAAGAAAATATTATTCATAATATACTAAATGAT
AATAAAAATTACAGATTAATTATTACAGGTCATAGTTTAGGTGCCGGAAT
CGCAATTATTTTATCGTGCTTTTATTATCACGAATTTCCTAATCTTAAAT
GTTATGCATTTAGCCCACCTCTGGGAACTGTTAATATTAAGACTGCAGAT
TACTGTAAAGATTTTGTCCTATCAATTGTCAATGGTGTTGAATGGGTTCC
TCGAATATCTTTACAATCAATTGTAGTGGCCAAACGCCGACTTATCGAAT
GTTTAGAAAATTATCCTCATTCTAAATTTCAATTATTAATTAAAAAATTT
ATCAAATGCAAATGTTGCTATTGTAGTAAAACATCCTGCAGATGGAACAT
ACTTAATTCATTTGAAAGAGATGAAAAATATTGGGAAAATATTGAAATAA
ATACAAAATTACCTGGACAAATAATACACATCACTAATATAAAAAAATTA
AATGATCCAAATAAATCTGATTTAATCAACAGCAACCGTAGCTTTTACAC
GCCCATAATTACTTCAAGCGATCAATTCAAAACAGTTGTATTTCATCCAA
AAATGTTTAAAGATCACTTTCCCCATCAAATGGTTAAAAGATTATTCAAC
ATTTTGTATGAAAACCAACTATTTTATCCAAATTGTCATCACTACTCAAA
GCATTATTGGCCAAGTTGTAAAGATAAAGATATTATGAAATTACCATTTA
TCAGTGGTATATTAAATTCCGATACGACATCACTCTCTTATTTTGCAACT
AATATTTGCAACCGAAAAAAGAGTAAAATACTAGAGGATTCTAATAATAG
TTTATATCTGATATCCAAAATTACAGTTGTGTGAAATTATTATATGCGCA
ATGGTAATAAATTGGTTTATCTTTTTAAAAAACA
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protein sequence of SMED30023344-orf-1

>SMED30023344-orf-1 ID=SMED30023344-orf-1|Name=SMED30023344-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=777bp
MPPLIMFGRKWNIATDDFVYLSFWEFLIRLFIIVAYVYFNFHENMINNCN
SKLYQICHFLLSGSLTCNLIIILAIMIMSAQGLIMEESKRSKIPFCIYIK
LFMSFYEAILSIVAFAINYRYSLIPNKFCEQILIPNKIILYCIGPFLTAY
LIIFCITYDQLGRHGSNVILLATKKTNIWKHRLKWILCIKKVNDEERNAL
DSAANIFASLCEESNLVFSDFEAGIQLLCKQDANAYDFTEYSLKINKSKS
GVTNSSSFNQKMYTIINHFINNEENYVNMSNFKKLANENLIETDSKVNNS
EVNLADKISTGNKSDLKSDWRDIRFIYYFSTMAKASYGWPIHLLFHDENK
SRLKFCMGLCCKHSTSCFCNKCICCDCEKSNSNDQKLIYVKERFCSCHEK
AILNETHLEKENLVCVSFYNKTFHSPFFIAISHEIEVIILSIRGTFSLKD
ILTDMDANLIEVFEDGFSGYLAHSGMLNAAKNIKRKIQEENIIHNILNDN
KNYRLIITGHSLGAGIAIILSCFYYHEFPNLKCYAFSPPLGTVNIKTADY
CKDFVLSIVNGVEWVPRISLQSIVVAKRRLIECLENYPHSKFQLLIKKFI
KCKCCYCSKTSCRWNILNSFERDEKYWENIEINTKLPGQIIHITNIKKLN
DPNKSDLINSNRSFYTPIITSSDQFKTVVFHPKMFKDHFPHQMVKRLFNI
LYENQLFYPNCHHYSKHYWPSCKDKDIMKLPFISGILNSDTTSLSYFATN
ICNRKKSKILEDSNNSLYLISKITVV*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000099neuron
PLANA:0000101muscle cell
PLANA:0000418head
PLANA:0002075pigment cell
PLANA:0002089reproductive organ
Vocabulary: INTERPRO
TermDefinition
IPR002921Fungal_lipase-like
IPR029058AB_hydrolase
Vocabulary: biological process
TermDefinition
GO:0006629lipid metabolic process
Vocabulary: cellular component
TermDefinition
GO:0016020membrane
GO:0016021integral component of membrane
Vocabulary: molecular function
TermDefinition
GO:0016787hydrolase activity
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePANTHERPTHR45792FAMILY NOT NAMEDcoord: 1..235
NoneNo IPR availablePANTHERPTHR45792:SF3SN1-SPECIFIC DIACYLGLYCEROL LIPASE ALPHAcoord: 1..235
NoneNo IPR availableTMHMMTMhelixcoord: 58..80
NoneNo IPR availableTMHMMTMhelixcoord: 20..42
NoneNo IPR availableTMHMMTMhelixcoord: 138..160
NoneNo IPR availableTMHMMTMhelixcoord: 95..117