Negative elongation factor A

Overview
NameNegative elongation factor A
Smed IDSMED30023296
Length (bp)2263
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Negative elongation factor A (SMED30023296) t-SNE clustered cells

Violin plots show distribution of expression levels for Negative elongation factor A (SMED30023296) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Negative elongation factor A (SMED30023296) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Negative elongation factor A (SMED30023296) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30023296

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 5

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30023296SMESG000066659.1 SMESG000066654.1 SMESG000006819.1 SmedASXL_014984SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
X1 cellSMED30023296SMESG000066659.1 SMESG000066654.1 SmedASXL_014983SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
X2 cellSMED30023296SMESG000066659.1 SMESG000066654.1 SmedASXL_014983SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
nervous systemSMED30023296SMESG000066659.1 SMESG000066654.1 dd_Smed_v4_8484_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30023296SMESG000066659.1 SMESG000066654.1 dd_Smed_v4_8484_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Negative elongation factor A vs. TrEMBL
Match: A0A5K4F0C1 (Uncharacterized protein OS=Schistosoma mansoni OX=6183 PE=4 SV=1)

HSP 1 Score: 128.642 bits (322), Expect = 1.111e-26
Identity = 107/381 (28.08%), Postives = 168/381 (44.09%), Query Frame = 2 
Query:   89 LIRSHFGSNLWTQISDINFFNDDRIKQINQCFKDLPVKSKLYILMALGNLNNNQFLKWKNEVESIFLRAMVEGNLLVRAIATILQQKFGLNYLTFDISLSLENFADILIKLTSNMEEWDVCCECIVAEYISENVVTEMMKHIEGSKSSAKIK----NRKRKLEVDDKFVNINEEKSQPNIGCKLKYLPKSYQIKNQYLQKIEAECNKKRISVPIPGRNKIKPKEENETDD-------------------------KDNNIKKEFQLIKETAQIRK-----ENEMNAKLEDIKKRLQQKSTTG-KKESKVICLSIEELPPTGPKAKQILRXXXXXXXXXXXXXXXXXXXXXXXXDKIRKLQERENKRIAQQMMLEQSKKEKA 1126
             +R  F  N WT+  D +  N+++++ I+  F  + VKSKL +L+A  N+  ++  +    +E+IFL+A+ E N LV+A+ATIL  K  LNYL FDIS + E F   + KL  +   W+     ++AEY+  NV+ +++      K  A +      R+ KLE                          S  +K  YL+K++ EC ++R+ VPIPGR+  +   ++ET+D                           +++  +  L    A I K     EN   +  + I  R    +  G KK S +  L  +ELP  GPKAKQ                             +RK +ERE K     M L+Q K+E+A
Sbjct:    7 FVRQQFFDNNWTKSIDPDSLNEEKLECIDASFSHMDVKSKLCLLLAFSNMKQSKVQQINKHIENIFLQALDEENNLVKAVATILHSKQTLNYLNFDISPTNEAFGKNIDKLLKSATNWNTVDTPLLAEYLGRNVLDKLLSLKGSDKPPASLSKCGFQRRGKLE--------------------------SEHLKESYLEKLKDECQRRRVHVPIPGRSFAR---QDETEDSVAVEVSDSTSFSRRKSTFPRISGQSSDDLDSKRSLTWARANINKTGSNSENRPISTNDRISSR--SPAMAGVKKHSGIKLLEFDELPAFGPKAKQ-----------------------------LRKEKERETK-----MKLKQEKEERA 322

HSP 2 Score: 72.7886 bits (177), Expect = 2.297e-9
Identity = 39/86 (45.35%), Postives = 57/86 (66.28%), Query Frame = 2 
Query: 1895 QLETMKILIRYANRLSRPQKAMIVSYIAGSADGV-DFNGQ-SSVHISSH--RAINLDTFQPLNVIVDAFFLMDYRTGRWSIVKQYR 2140
            QL T++ L + ANR++RP+KAMI+S+IAGS D      GQ + + +S H  R +N +T Q +++ VD F  MDY TG++  VK YR
Sbjct:  948 QLSTVQSLFQDANRVTRPEKAMIISFIAGSRDNPRPETGQITRIRLSEHRERVLNQNTGQLMDLAVDTFLTMDYSTGKYEKVKCYR 1033
[Close]
BLAST of Negative elongation factor A vs. TrEMBL
Match: G4VIS4 (Amidohydro-rel domain-containing protein OS=Schistosoma mansoni OX=6183 GN=Smp_024780.2 PE=4 SV=1)

HSP 1 Score: 127.102 bits (318), Expect = 3.957e-26
Identity = 106/380 (27.89%), Postives = 167/380 (43.95%), Query Frame = 2 
Query:   89 LIRSHFGSNLWTQISDINFFNDDRIKQINQCFKDLPVKSKLYILMALGNLNNNQFLKWKNEVESIFLRAMVEGNLLVRAIATILQQKFGLNYLTFDISLSLENFADILIKLTSNMEEWDVCCECIVAEYISENVVTEMMKHIEGSKSSAKIK----NRKRKLEVDDKFVNINEEKSQPNIGCKLKYLPKSYQIKNQYLQKIEAECNKKRISVPIPGRNKIKPKEENETDD-------------------------KDNNIKKEFQLIKETAQIRK-----ENEMNAKLEDIKKRLQQKSTTGKKESKVICLSIEELPPTGPKAKQILRXXXXXXXXXXXXXXXXXXXXXXXXDKIRKLQERENKRIAQQMMLEQSKKEKA 1126
             +R  F  N WT+  D +  N+++++ I+  F  + VKSKL +L+A  N+  ++  +    +E+IFL+A+ E N LV+A+ATIL  K  LNYL FDIS + E F   + KL  +   W+     ++AEY+  NV+ +++      K  A +      R+ KLE                          S  +K  YL+K++ EC ++R+ VPIPGR+  +   ++ET+D                           +++  +  L    A I K     EN   +  + I  R    +   KK S +  L  +ELP  GPKAKQ                             +RK +ERE K     M L+Q K+E+A
Sbjct:    7 FVRQQFFDNNWTKSIDPDSLNEEKLECIDASFSHMDVKSKLCLLLAFSNMKQSKVQQINKHIENIFLQALDEENNLVKAVATILHSKQTLNYLNFDISPTNEAFGKNIDKLLKSATNWNTVDTPLLAEYLGRNVLDKLLSLKGSDKPPASLSKCGFQRRGKLE--------------------------SEHLKESYLEKLKDECQRRRVHVPIPGRSFAR---QDETEDSVAVEVSDSTSFSRRKSTFPRISGQSSDDLDSKRSLTWARANINKTGSNSENRPISTNDRISSRSPAMAGV-KKHSGIKLLEFDELPAFGPKAKQ-----------------------------LRKEKERETK-----MKLKQEKEERA 322

HSP 2 Score: 71.2478 bits (173), Expect = 8.145e-9
Identity = 39/86 (45.35%), Postives = 57/86 (66.28%), Query Frame = 2 
Query: 1895 QLETMKILIRYANRLSRPQKAMIVSYIAGSADGV-DFNGQ-SSVHISSH--RAINLDTFQPLNVIVDAFFLMDYRTGRWSIVKQYR 2140
            QL T++ L + ANR++RP+KAMI+S+IAGS D      GQ + + +S H  R +N +T Q +++ VD F  MDY TG++  VK YR
Sbjct:  941 QLSTVQSLFQDANRVTRPEKAMIISFIAGSRDNPRPETGQITRIRLSEHRERVLNQNTGQLMDLAVDTFLTMDYSTGKYEKVKCYR 1026
[Close]
BLAST of Negative elongation factor A vs. TrEMBL
Match: A0A430QK62 (Negative elongation factor A OS=Schistosoma bovis OX=6184 GN=DC041_0000963 PE=4 SV=1)

HSP 1 Score: 125.561 bits (314), Expect = 1.415e-25
Identity = 108/392 (27.55%), Postives = 168/392 (42.86%), Query Frame = 2 
Query:   89 LIRSHFGSNLWTQISDINFFNDDRIKQINQCFKDLPVKSKLYILMALGNLNNNQFLKWKNEVESIFLRAMVEGNLLVRAIATILQQKFGLNYLTFDISLSLENFADILIKLTSNMEEWDVCCECIVAEYISENVVTEMMKHIEGSKSSAKIKNRKRKLEVDDKFVNIN----EEKSQPNIGCKLKYLPKSYQIKNQYLQKIEAECNKKRISVPIPGRNKIKPKEENETDD-------------------------------------KDNNIKKEFQLIKETAQIRK-----ENEMNAKLEDIKKRLQQKSTTGKKESKVICLSIEELPPTGPKAKQILRXXXXXXXXXXXXXXXXXXXXXXXXDKIRKLQERENKRIAQQMMLEQSKKEKA 1126
             +R  F  N WT+  D +  N+++++ I+  F  + VKSKL +L+A  N+  +   +    +E+IFL+A+ E N LV+A+ATIL  K  LNYL FDIS + E F   + KL  +   W+     ++AEY+  NV+                          DKF+N+        S P  G + +   +S  +K  YL+K++ EC ++R+ VPIPGR+  +   ++ET+D                                       +++  +  LI   A I K     EN   +  + I  R    +   KK S +  L  +ELP  GPKAKQ                             +RK +ERE K     M L+Q K+E+A
Sbjct:    7 FVRQQFFDNNWTKSIDPDSLNEEKLECIDASFSHMDVKSKLCLLLAFSNMKQSTVQQINKHIENIFLQALDEENNLVKAVATILHSKQTLNYLNFDISPTNEAFGKNIDKLLKSATNWNTVDTPLLAEYLGRNVL--------------------------DKFLNLKGNDKPPASLPKCGFQRRGKLESEHLKESYLEKLKDECQRRRVHVPIPGRSFAR---QDETEDSVAVEVVSNAGNSVTSSQSDSTSFSRRKSTFPRISGQSSDDLDSKRSLIWTRANINKTGNNSENRPISTNDRISSRSPAMAGV-KKHSGIKLLEFDELPAFGPKAKQ-----------------------------LRKEKERETK-----MKLKQEKEERA 334

HSP 2 Score: 70.8626 bits (172), Expect = 1.153e-8
Identity = 38/86 (44.19%), Postives = 57/86 (66.28%), Query Frame = 2 
Query: 1895 QLETMKILIRYANRLSRPQKAMIVSYIAGSADGV-DFNGQ-SSVHISSH--RAINLDTFQPLNVIVDAFFLMDYRTGRWSIVKQYR 2140
            QL T++ L + ANR++RP+KAMI+S+IAGS D      GQ + + +S H  R +N +T Q +++ VD F  MDY TG++  +K YR
Sbjct:  960 QLSTVQSLFQDANRVTRPEKAMIISFIAGSRDNPRPETGQITRIRLSEHRERVLNQNTGQLMDLAVDTFLTMDYSTGKYEKIKCYR 1045
[Close]
BLAST of Negative elongation factor A vs. TrEMBL
Match: A0A094ZGZ9 (Negative elongation factor A OS=Schistosoma haematobium OX=6185 GN=MS3_00243 PE=4 SV=1)

HSP 1 Score: 124.79 bits (312), Expect = 2.110e-25
Identity = 108/392 (27.55%), Postives = 168/392 (42.86%), Query Frame = 2 
Query:   89 LIRSHFGSNLWTQISDINFFNDDRIKQINQCFKDLPVKSKLYILMALGNLNNNQFLKWKNEVESIFLRAMVEGNLLVRAIATILQQKFGLNYLTFDISLSLENFADILIKLTSNMEEWDVCCECIVAEYISENVVTEMMKHIEGSKSSAKIKNRKRKLEVDDKFVNIN----EEKSQPNIGCKLKYLPKSYQIKNQYLQKIEAECNKKRISVPIPGRNKIKPKEENETDD-------------------------------------KDNNIKKEFQLIKETAQIRK-----ENEMNAKLEDIKKRLQQKSTTGKKESKVICLSIEELPPTGPKAKQILRXXXXXXXXXXXXXXXXXXXXXXXXDKIRKLQERENKRIAQQMMLEQSKKEKA 1126
             +R  F  N WT+  D +  N+++++ I+  F  + VKSKL +L+A  N+  +   +    +E+IFL+A+ E N LV+A+ATIL  K  LNYL FDIS + E F   + KL  +   W+     ++AEY+  NV+                          DKF+N+        S P  G + +   +S  +K  YL+K++ EC ++R+ VPIPGR+  +   ++ET+D                                       +++  +  LI   A I K     EN   +  + I  R    +   KK S +  L  +ELP  GPKAKQ                             +RK +ERE K     M L+Q K+E+A
Sbjct:    7 FVRQQFFDNNWTKSIDPDSLNEEKLECIDASFSHMDVKSKLCLLLAFSNVKQSTVQQINKHIENIFLQALDEENNLVKAVATILHSKQTLNYLNFDISPTNEAFGKNIDKLLKSATNWNTVDTPLLAEYLGRNVL--------------------------DKFLNLKGNDKPPASLPKCGFQRRGKLESEHLKESYLEKLKDECQRRRVHVPIPGRSFAR---QDETEDSVAVEVVSNAGNSVTSSQSDSTSFSRRKSTFPRISGQSSDDLDSKRSLIWTRANINKTGNNSENRPISTNDRISSRSPAMAGV-KKHSGIKLLEFDELPAFGPKAKQ-----------------------------LRKEKERETK-----MKLKQEKEERA 334

HSP 2 Score: 70.4774 bits (171), Expect = 1.657e-8
Identity = 38/86 (44.19%), Postives = 57/86 (66.28%), Query Frame = 2 
Query: 1895 QLETMKILIRYANRLSRPQKAMIVSYIAGSADGV-DFNGQ-SSVHISSH--RAINLDTFQPLNVIVDAFFLMDYRTGRWSIVKQYR 2140
            QL T++ L + ANR++RP+KAMI+S+IAGS D      GQ + + +S H  R +N +T Q +++ VD F  MDY TG++  +K YR
Sbjct:  960 QLSTVQSLFQDANRVTRPEKAMIISFIAGSRDNPRPETGQITRIRLSEHRERVLNQNTGQLVDLAVDTFLTMDYSTGKYEKIKCYR 1045
[Close]
BLAST of Negative elongation factor A vs. TrEMBL
Match: A0A5J4NC23 (Negative elongation factor A (Fragment) OS=Paragonimus westermani OX=34504 GN=DEA37_0002803 PE=4 SV=1)

HSP 1 Score: 124.02 bits (310), Expect = 3.885e-25
Identity = 110/425 (25.88%), Postives = 193/425 (45.41%), Query Frame = 2 
Query:   89 LIRSHFGSNLWTQISDINFFNDDRIKQINQCFKDLPVKSKLYILMALGNLNNNQFLKWKNEVESIFLRAMVEGNLLVRAIATILQQKFGLNYLTFDISLSLENFADILIKLTSNMEEWDVCCECIVAEYISENVVTEMMKHIEGSKSSAKIKNRKRKLEVDDKFVNINEEKSQPNIGCKLKYLPKSYQIKNQYLQKIEAECNKKRISVPIPGRNKIKPKEENETDDKDN---------------------------------------NIKKEFQLIKETAQIRKENEMNAKL--EDIKKRLQQKSTTGKKESKVICLSIEELPPTGPKAKQILRXXXXXXXXXXXXXXXXXXXXXXXXDKIRKLQERENKRIAQQMMLEQSKKEK-AEMEMQFNANIIDSNGGLPITDTVVDEENYLSMPGSYS 1237
             IR HF  N WT+  D    ++++   I+  F ++ VKSKL +L++  +L  +   +    +E+IFL+A+ E N LV+A+ATIL  K  LNYL FDIS + E F   + +L  +  +W V    ++AE++ E V+  ++ +   +K S     R+    V    V +N     P+  C+ K  P+S  +K  YL++++ EC ++R+ VPIPGR+  + +E ++  D ++                                       N+   +    + A   + NE+ +    E +  R    +   +K S +  L  +ELP  GPKAKQ+ +                   E+ E ++  K QERE++   Q+   E SK+ + AE +   + +    +   P   +   +    S+PG+ S
Sbjct:    7 FIRQHFFDNQWTREVDPTTLSEEKWVSIDSAFCNIDVKSKLCLLLSFCHLKPSLPPEINKHIENIFLQALDEENNLVKAVATILHSKQSLNYLNFDISPTNEAFRKNIQRLLKSSADWRVVGTPLLAEFLGEGVLA-LVSNGPLTKHSVPSTKRQCASSVSGP-VKVN-----PSFRCRGK--PQSASLKESYLERLKDECQRRRVHVPIPGRSFARSEEADDDHDLESSGFLGSQMTIPRRSLTPGQNKKGGVAPSSWSQSSDNSGRNLVGSWSRSNQDAVPERSNEVRSAFVSEKLAARFPAAANF-RKSSGIKLLDFDELPAFGPKAKQLRK-------------------EQMEKEREVKRQEREDRLKEQKAAREASKRARQAERQALVSRSHAPVSSHWPALPSTGSDMPITSIPGAIS 402
[Close]
BLAST of Negative elongation factor A vs. Ensembl Sea Lamprey
Match: ENSPMAT00000000497.1 (pep scaffold:Pmarinus_7.0:GL485460:152936:167370:1 gene:ENSPMAG00000000435.1 transcript:ENSPMAT00000000497.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 51.9878 bits (123), Expect = 3.438e-7
Identity = 25/86 (29.07%), Postives = 49/86 (56.98%), Query Frame = 2 
Query: 1895 QLETMKILIRYANRLSRPQKAMIVSYIAGSAD--GVDFNGQSSVHISSHRAI--NLDTFQPLNVIVDAFFLMDYRTGRWSIVKQYR 2140
            Q+   + + + AN+++RP+KA+I+ ++AGS +    D      + +S H  +    D      ++VD  F M+Y TG+W+ +K+Y+
Sbjct:  380 QMYAAQEMFKTANKVTRPEKALILGFMAGSRENPCPDQGDIIQIKLSEHTEVLPKADGTGSTTMLVDTVFEMNYATGQWTRLKKYK 465
[Close]
BLAST of Negative elongation factor A vs. Planmine SMEST
Match: SMESG000066654.1 (SMESG000066654.1)

HSP 1 Score: 1252.27 bits (3239), Expect = 0.000e+0
Identity = 716/721 (99.31%), Postives = 719/721 (99.72%), Query Frame = 2 
Query:   56 MTTSNLSIVDSLIRSHFGSNLWTQISDINFFNDDRIKQINQCFKDLPVKSKLYILMALGNLNNNQFLKWKNEVESIFLRAMVEGNLLVRAIATILQQKFGLNYLTFDISLSLENFADILIKLTSNMEEWDVCCECIVAEYISENVVTEMMKHIEGSKSSAKIKNRKRKLEVDDKFVNINEEKSQPNIGCKLKYLPKSYQIKNQYLQKIEAECNKKRISVPIPGRNKIKPKEENETDDKDNNIKKEFQLIKETAQIRKENEMNAKLEDIKKRLQQKSTTGKKESKVICLSIEELPPTGPKAKQILRXXXXXXXXXXXXXXXXXXXXXXXXDKIRKLQERENKRIAQQMMLEQSKKEKAEMEMQFNANIIDSNGGLPITDTVVDEENYLSMPGSYSRPPGSAXXXXXXXXXXXXXXXXXXXXFVNQLPAQIRLVQSDQTGYRIIDKPSANIRFIPNQNMSQSVFRXXXXXXXXXXXXXXXXXXXXXXXXXXNTNFSASSANQISQQPSYILSTNQAGVKLVRLVSNTNQNISQPSQQRLVRIIGSTSQSNNKNPACSSTPTINISRNFSQSQISPVSVXXXXXXXXXXXXXXXXXXXXATHINNSQQIHVNPQSMQLETMKILIRYANRLSRPQKAMIVSYIAGSADGVDFNGQSSVHISSHRAINLDTFQPLNVIVDAFFLMDYRTGRWSIVKQYREPRNEEDICSFDLQPSGYPPEFSMQA 2218
            MTTSNLSIVDSLIRSHFGSNLWTQI+DINFFNDDRIKQINQCFKDLPVKSKLYILMALGNLNNNQFLKWKNEVESIFLRAMVEGNLLVRAIATILQQKFGLNYLTFDISLSLENFADILIKLTSNMEEWDVCCECIVAEYISENVVTEMMKHIEGSKSSAKIK+RKRKLEVDDK VNINEEKSQPNIGCKLKYLPKSYQIKNQYLQKIEAECNKKRISVPIPGRNKIKPKEENETDDKDNNIKKEFQLIKETAQIRKENEMNAKLEDIKKRLQQKSTTGKKESKVICLSIEELPPTGPKAKQILREKEKEAKKVEKEEKLKKVKEEKEADKIRKLQERENKRIAQQMMLEQSKKEKAEMEMQFNANIIDSNGGLPITDTVVDEENYLS+PGSYSRPPGSAPQSIQQAPSLLPPPPTATTQFVNQLPAQIRLVQSDQTGYRIIDKPSANIRFIPNQNMSQSVFRPPQPPPQQ QSQQPQIVLIQSPSQIQNTNFSASSANQISQQPSYILSTNQAGVKLVRLVSNTNQNISQPSQQRLVRIIGSTSQSNNKNPACSSTPTINISRNFSQSQISPVSVNNISQPPPLQQQPQINQQLPATHINNSQQIHVNPQSMQLETMKILIRYANRLSRPQKAMIVSYIAGSADGVDFNGQSSVHISSHRAINLDTFQPLNVIVDAFFLMDYRTGRWSIVKQYREPRNEEDICSFDLQPSGYPPEFSMQA
Sbjct:    1 MTTSNLSIVDSLIRSHFGSNLWTQITDINFFNDDRIKQINQCFKDLPVKSKLYILMALGNLNNNQFLKWKNEVESIFLRAMVEGNLLVRAIATILQQKFGLNYLTFDISLSLENFADILIKLTSNMEEWDVCCECIVAEYISENVVTEMMKHIEGSKSSAKIKSRKRKLEVDDKIVNINEEKSQPNIGCKLKYLPKSYQIKNQYLQKIEAECNKKRISVPIPGRNKIKPKEENETDDKDNNIKKEFQLIKETAQIRKENEMNAKLEDIKKRLQQKSTTGKKESKVICLSIEELPPTGPKAKQILREKEKEAKKVEKEEKLKKVKEEKEADKIRKLQERENKRIAQQMMLEQSKKEKAEMEMQFNANIIDSNGGLPITDTVVDEENYLSVPGSYSRPPGSAPQSIQQAPSLLPPPPTATTQFVNQLPAQIRLVQSDQTGYRIIDKPSANIRFIPNQNMSQSVFRPPQPPPQQSQSQQPQIVLIQSPSQIQNTNFSASSANQISQQPSYILSTNQAGVKLVRLVSNTNQNISQPSQQRLVRIIGSTSQSNNKNPACSSTPTINISRNFSQSQISPVSVNNISQPPPLQQQPQINQQLPATHINNSQQIHVNPQSMQLETMKILIRYANRLSRPQKAMIVSYIAGSADGVDFNGQSSVHISSHRAINLDTFQPLNVIVDAFFLMDYRTGRWSIVKQYREPRNEEDICSFDLQPSGYPPEFSMQA 721
[Close]
BLAST of Negative elongation factor A vs. Planmine SMEST
Match: SMESG000066659.1 (SMESG000066659.1)

HSP 1 Score: 1189.1 bits (3075), Expect = 0.000e+0
Identity = 697/725 (96.14%), Postives = 701/725 (96.69%), Query Frame = 2 
Query:   44 RLIFMTTSNLSIVDSLIRSHFGSNLWTQISDINFFNDDRIKQINQCFKDLPVKSKLYILMALGNLNNNQFLKWKNEVESIFLRAMVEGNLLVRAIATILQQKFGLNYLTFDISLSLENFADILIKLTSNMEEWDVCCECIVAEYISENVVTEMMKHIEGSKSSAKIKNRKRKLEVDDKFVNINEEKSQPNIGCKLKYLPKSYQIKNQYLQKIEAECNKKRISVPIPGRNKIKPKEENETDDKDNNIKKEFQLIKETAQIRKENEMNAKLEDIKKRLQQKSTTGKKESKVICLSIEELPPTGPKAKQILRXXXXXXXXXXXXXXXXXXXXXXXXDKIRKLQERENKRIAQQMMLEQSKKEKAEMEMQFNANIIDSNGGLPITDTVVDEENYLSMPGSYSRPPGSAXXXXXXXXXXXXXXXXXXXXFVNQLPAQIRLVQSDQTGYRIIDKPSANIRFIPNQNMSQSVFRXXXXXXXXXXXXXXXXXXXXXXXXXXNTNFSASSANQISQQPSYILSTNQAGVKLVRLVSNTNQNISQPSQQRLVRIIGSTSQSNNKNPACSSTPTINISRNFSQSQISPVSVXXXXXXXXXXXXXXXXXXXXATHINNSQQIHVNPQSMQLETMKILIRYANRLSRPQKAMIVSYIAGSADGVDFNGQSSVHISSHRAINLDTFQPLNVIVDAFFLMDYRTGRWSIVKQYREPRNEEDICSFDLQPSGYPPEFSMQA 2218
            +LIFMTTSNLSIVDSLIRSHFGSNLWTQI+DINFFNDDRIKQINQCFKDLPVKSKLYILMALGNLNNNQFLKWKNEVESIFLRAMVEGNLLVRAIATILQQKFGLNYLTFDISLSLENFADILIKLTSNMEEWDVCCECIVAEYISENVVTEMMKHIEGSKSSAKIK+RKRKLEVDDK VNINEEKSQPNIGCKLKYLPKSYQIKNQYLQKIEAECNKKRISVPIPGRNKIKPKEENETDDKDNNIKKEFQLIKETAQIRKENEMNAKLEDIKKRLQQKSTTGKKESK                      KEKEAKKVEKEEKLKKVKEEKEADKIRKLQERENKRIAQQMMLEQSKKEKAEMEMQFNANIIDSNGGLPITDTVVDEENYLS+PGSYSRPPGSAPQSIQQAPSLLPPPPTATTQFVNQLPAQIRLVQSDQTGYRIIDKPSANIRFIPNQNMSQSVFRPPQPPPQQ QSQQPQIVLIQSPSQIQNTNFSASSANQISQQPSYILSTNQAGVKLVRLVSNTNQNISQPSQQRLVRIIGSTSQSNNKNPACSSTPTINISRNFSQSQISPVSVNNISQPPPLQQQPQINQQLPATHINNSQQIHVNPQSMQLETMKILIRYANRLSRPQKAMIVSYIAGSADGVDFNGQSSVHISSHRAINLDTFQPLNVIVDAFFLMDYRTGRWSIVKQYREPRNEEDICSFDLQPSGYPPEFSMQA
Sbjct:   64 KLIFMTTSNLSIVDSLIRSHFGSNLWTQITDINFFNDDRIKQINQCFKDLPVKSKLYILMALGNLNNNQFLKWKNEVESIFLRAMVEGNLLVRAIATILQQKFGLNYLTFDISLSLENFADILIKLTSNMEEWDVCCECIVAEYISENVVTEMMKHIEGSKSSAKIKSRKRKLEVDDKIVNINEEKSQPNIGCKLKYLPKSYQIKNQYLQKIEAECNKKRISVPIPGRNKIKPKEENETDDKDNNIKKEFQLIKETAQIRKENEMNAKLEDIKKRLQQKSTTGKKESKR---------------------KEKEAKKVEKEEKLKKVKEEKEADKIRKLQERENKRIAQQMMLEQSKKEKAEMEMQFNANIIDSNGGLPITDTVVDEENYLSVPGSYSRPPGSAPQSIQQAPSLLPPPPTATTQFVNQLPAQIRLVQSDQTGYRIIDKPSANIRFIPNQNMSQSVFRPPQPPPQQSQSQQPQIVLIQSPSQIQNTNFSASSANQISQQPSYILSTNQAGVKLVRLVSNTNQNISQPSQQRLVRIIGSTSQSNNKNPACSSTPTINISRNFSQSQISPVSVNNISQPPPLQQQPQINQQLPATHINNSQQIHVNPQSMQLETMKILIRYANRLSRPQKAMIVSYIAGSADGVDFNGQSSVHISSHRAINLDTFQPLNVIVDAFFLMDYRTGRWSIVKQYREPRNEEDICSFDLQPSGYPPEFSMQA 767
The following BLAST results are available for this feature:
BLAST of Negative elongation factor A vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Negative elongation factor A vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Negative elongation factor A vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Negative elongation factor A vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Negative elongation factor A vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Negative elongation factor A vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Negative elongation factor A vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Negative elongation factor A vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A5K4F0C11.111e-2628.08Uncharacterized protein OS=Schistosoma mansoni OX=... [more]
G4VIS43.957e-2627.89Amidohydro-rel domain-containing protein OS=Schist... [more]
A0A430QK621.415e-2527.55Negative elongation factor A OS=Schistosoma bovis ... [more]
A0A094ZGZ92.110e-2527.55Negative elongation factor A OS=Schistosoma haemat... [more]
A0A5J4NC233.885e-2525.88Negative elongation factor A (Fragment) OS=Paragon... [more]
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BLAST of Negative elongation factor A vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Negative elongation factor A vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ENSPMAT00000000497.13.438e-729.07pep scaffold:Pmarinus_7.0:GL485460:152936:167370:1... [more]
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BLAST of Negative elongation factor A vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Negative elongation factor A vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Negative elongation factor A vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Negative elongation factor A vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000066654.10.000e+099.31SMESG000066654.1[more]
SMESG000066659.10.000e+096.14SMESG000066659.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30023296 ID=SMED30023296|Name=Negative elongation factor A|organism=Schmidtea mediterranea sexual|type=transcript|length=2263bp
TTCTGACTAAACTTCTTTTTTTCAAAAGTGTAATTTTTACATTAGATTGA
TTTTTATGACAACTAGTAATTTATCCATTGTTGATTCTTTGATTAGAAGT
CACTTTGGCTCCAATCTATGGACTCAAATTTCTGATATCAATTTTTTCAA
TGATGATCGGATCAAACAAATTAATCAATGTTTTAAAGATTTACCTGTTA
AATCAAAATTATACATCTTAATGGCTCTTGGAAATTTGAACAATAATCAA
TTTTTGAAATGGAAAAATGAAGTTGAATCCATATTTCTAAGGGCCATGGT
TGAGGGTAATCTTTTAGTTCGAGCAATCGCTACCATTTTACAACAGAAAT
TTGGTCTAAATTATTTAACATTTGATATTAGTTTAAGTCTCGAGAATTTC
GCTGATATTTTGATTAAATTAACTTCCAATATGGAAGAATGGGATGTTTG
CTGTGAGTGCATAGTAGCGGAATATATCAGTGAAAATGTTGTTACTGAAA
TGATGAAACACATCGAAGGATCTAAATCTAGCGCTAAGATAAAAAATAGA
AAAAGGAAATTAGAAGTTGATGATAAGTTTGTCAATATAAATGAAGAAAA
ATCGCAGCCAAATATCGGTTGCAAATTGAAGTATTTGCCAAAATCCTATC
AAATTAAAAATCAATATTTACAAAAAATTGAAGCCGAGTGTAATAAAAAA
CGAATTAGTGTTCCAATTCCTGGGCGAAATAAAATCAAACCAAAAGAAGA
AAATGAAACCGACGATAAAGACAACAACATTAAAAAAGAATTCCAATTGA
TTAAAGAAACGGCTCAAATACGAAAAGAAAATGAAATGAATGCGAAATTG
GAGGATATCAAAAAGCGTTTGCAACAGAAATCGACTACTGGAAAGAAAGA
ATCGAAAGTTATTTGTCTAAGTATAGAAGAGCTGCCGCCGACCGGACCAA
AGGCCAAGCAAATTCTCAGAGAAAAAGAAAAAGAAGCAAAAAAGGTTGAA
AAGGAAGAAAAATTAAAAAAAGTCAAAGAAGAAAAGGAAGCAGACAAAAT
TCGAAAGCTGCAAGAAAGAGAAAACAAACGAATTGCCCAACAAATGATGC
TGGAGCAATCGAAAAAGGAGAAGGCAGAAATGGAAATGCAATTTAACGCC
AACATTATCGATTCGAACGGAGGATTGCCAATCACTGACACTGTCGTTGA
CGAGGAGAATTACCTGTCGATGCCCGGTAGCTACTCCAGGCCTCCTGGTT
CTGCTCCTCAGTCGATCCAGCAAGCCCCCTCATTGCTGCCACCCCCACCC
ACTGCGACCACGCAATTCGTCAATCAACTACCCGCACAAATCAGACTTGT
CCAATCCGATCAGACCGGCTATAGGATAATCGATAAACCTAGCGCTAACA
TTCGATTCATTCCAAATCAGAATATGTCCCAGTCAGTGTTCCGGCCTCCG
CAACCCCCACCCCAACAACCGCAATCACAACAGCCACAAATAGTCCTTAT
CCAATCACCGTCACAGATACAGAATACAAACTTCTCAGCCTCGTCAGCCA
ATCAGATCTCTCAGCAGCCCAGCTATATCCTGTCGACCAATCAAGCCGGT
GTGAAATTAGTCCGACTTGTCAGTAACACCAATCAGAACATTTCCCAGCC
ATCCCAGCAGCGACTCGTACGTATTATTGGATCTACTAGTCAGTCGAATA
ATAAGAATCCAGCTTGCAGCTCAACTCCAACGATCAATATTTCCCGCAAC
TTCTCACAGTCACAGATCAGTCCGGTCTCAGTCAATAACATTTCTCAGCC
ACCGCCGCTCCAGCAGCAGCCACAGATCAACCAGCAACTCCCAGCTACTC
ATATCAACAATTCTCAGCAGATTCATGTCAATCCTCAATCAATGCAGCTT
GAAACGATGAAAATTCTCATTCGATATGCCAATCGGCTCTCCCGACCCCA
GAAAGCCATGATTGTTTCTTACATTGCAGGCTCGGCAGACGGTGTCGATT
TCAATGGCCAATCCTCGGTACACATCAGTTCCCATCGTGCAATAAATCTC
GACACATTCCAACCGTTGAATGTGATTGTCGATGCGTTCTTCCTAATGGA
CTATCGAACCGGCCGATGGTCAATAGTCAAGCAATACCGGGAGCCACGGA
ACGAGGAGGACATTTGCAGTTTCGATCTGCAGCCTAGTGGTTACCCGCCG
GAATTCTCTATGCAGGCCTGATCGATAGATTCCTATTGCAAATATCTCGT
GTTTTATTTAATT
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protein sequence of SMED30023296-orf-1

>SMED30023296-orf-1 ID=SMED30023296-orf-1|Name=SMED30023296-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=722bp
MTTSNLSIVDSLIRSHFGSNLWTQISDINFFNDDRIKQINQCFKDLPVKS
KLYILMALGNLNNNQFLKWKNEVESIFLRAMVEGNLLVRAIATILQQKFG
LNYLTFDISLSLENFADILIKLTSNMEEWDVCCECIVAEYISENVVTEMM
KHIEGSKSSAKIKNRKRKLEVDDKFVNINEEKSQPNIGCKLKYLPKSYQI
KNQYLQKIEAECNKKRISVPIPGRNKIKPKEENETDDKDNNIKKEFQLIK
ETAQIRKENEMNAKLEDIKKRLQQKSTTGKKESKVICLSIEELPPTGPKA
KQILREKEKEAKKVEKEEKLKKVKEEKEADKIRKLQERENKRIAQQMMLE
QSKKEKAEMEMQFNANIIDSNGGLPITDTVVDEENYLSMPGSYSRPPGSA
PQSIQQAPSLLPPPPTATTQFVNQLPAQIRLVQSDQTGYRIIDKPSANIR
FIPNQNMSQSVFRPPQPPPQQPQSQQPQIVLIQSPSQIQNTNFSASSANQ
ISQQPSYILSTNQAGVKLVRLVSNTNQNISQPSQQRLVRIIGSTSQSNNK
NPACSSTPTINISRNFSQSQISPVSVNNISQPPPLQQQPQINQQLPATHI
NNSQQIHVNPQSMQLETMKILIRYANRLSRPQKAMIVSYIAGSADGVDFN
GQSSVHISSHRAINLDTFQPLNVIVDAFFLMDYRTGRWSIVKQYREPRNE
EDICSFDLQPSGYPPEFSMQA*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000099neuron
PLANA:0000418head
PLANA:0002109X1 cell
PLANA:0002111X2 cell
Vocabulary: molecular function
TermDefinition
GO:0003746translation elongation factor activity
GO:0016787hydrolase activity
Vocabulary: biological process
TermDefinition
GO:0006414translational elongation
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 255..278
NoneNo IPR availableCOILSCoilCoilcoord: 300..333