hypothetical protein
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30023292 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 3
Homology
BLAST of hypothetical protein vs. TrEMBL
Match: I1ZI77 (Uncharacterized protein OS=Schmidtea mediterranea OX=79327 PE=2 SV=1) HSP 1 Score: 492.656 bits (1267), Expect = 2.484e-174 Identity = 233/234 (99.57%), Postives = 234/234 (100.00%), Query Frame = 2 Query: 131 MGGLLSRRKKPPGQKCKFQNPLPAHEAENVLFMAEEQNNANVYAINSPNRQPRSIRNQTLLDTPNFNARIEPVAKEGNQVSKTHYFLIEEPKYRRCNSSKERCNPIEMYANSSRLFNQFCEEPLLPFDMNMHITHEHNSLQVRRGRSDTTDYLYNQQRAMVAQMQMKIPQATYVESHGVLMGTNWNFEEAERRLKMELVHRAIGANYKQCEALLIKHSWNLQCAMSSLYDHDPN 832 MGGLLSRRKKPPGQKCKFQNPLPAHEAENVLF+AEEQNNANVYAINSPNRQPRSIRNQTLLDTPNFNARIEPVAKEGNQVSKTHYFLIEEPKYRRCNSSKERCNPIEMYANSSRLFNQFCEEPLLPFDMNMHITHEHNSLQVRRGRSDTTDYLYNQQRAMVAQMQMKIPQATYVESHGVLMGTNWNFEEAERRLKMELVHRAIGANYKQCEALLIKHSWNLQCAMSSLYDHDPN Sbjct: 1 MGGLLSRRKKPPGQKCKFQNPLPAHEAENVLFIAEEQNNANVYAINSPNRQPRSIRNQTLLDTPNFNARIEPVAKEGNQVSKTHYFLIEEPKYRRCNSSKERCNPIEMYANSSRLFNQFCEEPLLPFDMNMHITHEHNSLQVRRGRSDTTDYLYNQQRAMVAQMQMKIPQATYVESHGVLMGTNWNFEEAERRLKMELVHRAIGANYKQCEALLIKHSWNLQCAMSSLYDHDPN 234
BLAST of hypothetical protein vs. TrEMBL
Match: A0A094ZG11 (Uncharacterized protein OS=Schistosoma haematobium OX=6185 GN=MS3_01614 PE=4 SV=1) HSP 1 Score: 87.4261 bits (215), Expect = 4.403e-15 Identity = 57/183 (31.15%), Postives = 95/183 (51.91%), Query Frame = 2 Query: 311 LDTPNFNARIEPVAKEGNQVSKTHYFLIEEPKYR--RCNSSKER----------CNPIEMYANSSRLFN----QFCEEPLLPFDMNMHITHEHNSLQVRRGRSDTT-DYLYNQQRAMVAQMQMKIPQATYVESHGVLMGTNWNFEEAERRLKMELVHRAIGANYKQCEALLIKHSWNLQCAMS 808 L FN RI P+ + G ++S THY+L++ P+ + R NS+ R +P+++Y+ N C LP +++RRG+S+ D L N R +V ++ ++PQAT E VL+G+++++EEA RRLK EL+ R + + LL K++W+L A+S Sbjct: 321 LGNSRFNVRIYPIIQNGVKISDTHYWLLDPPRSKTLRDNSTLMRRTTECFRVPPSSPLDIYSTIDGYTNIESTSLCPPNELPL----------KDVKLRRGKSENCKDSLKNFHRELVLRLMKEVPQATESECQAVLIGSSYDYEEATRRLKFELLCRKGYVSRSHSKRLLHKYNWDLDAAIS 493
BLAST of hypothetical protein vs. TrEMBL
Match: A0A430Q980 (Uncharacterized protein OS=Schistosoma bovis OX=6184 GN=DC041_0003318 PE=4 SV=1) HSP 1 Score: 87.0409 bits (214), Expect = 4.569e-15 Identity = 57/183 (31.15%), Postives = 95/183 (51.91%), Query Frame = 2 Query: 311 LDTPNFNARIEPVAKEGNQVSKTHYFLIEEPKYR--RCNSSKER----------CNPIEMYANSSRLFN----QFCEEPLLPFDMNMHITHEHNSLQVRRGRSDTT-DYLYNQQRAMVAQMQMKIPQATYVESHGVLMGTNWNFEEAERRLKMELVHRAIGANYKQCEALLIKHSWNLQCAMS 808 L FN RI P+ + G ++S THY+L++ P+ + R NS+ R +P+++Y+ N C LP +++RRG+S+ D L N R +V ++ ++PQAT E VL+G+++++EEA RRLK EL+ R + + LL K++W+L A+S Sbjct: 321 LGNSRFNVRIYPIIQNGVKISDTHYWLLDPPRSKTLRDNSTLMRRTTECFRVPPSSPLDIYSTIDGYTNIESTSLCPPNELPL----------KDVKLRRGKSENCKDSLKNFHRELVLRLMKEVPQATESECQAVLIGSSYDYEEATRRLKFELLCRKGYVSRSHSKRLLHKYNWDLDAAIS 493
BLAST of hypothetical protein vs. TrEMBL
Match: A0A4S2LFZ5 (Uncharacterized protein OS=Opisthorchis felineus OX=147828 GN=CRM22_007891 PE=4 SV=1) HSP 1 Score: 85.8853 bits (211), Expect = 1.100e-14 Identity = 55/164 (33.54%), Postives = 84/164 (51.22%), Query Frame = 2 Query: 326 FNARIEPVAKEGNQVSKTHYFLIEEPKYRRCNSSKERCNPIEMYANSSRLFNQFCEEPLLPF--DMNMHITHEHNSLQVRRGRSDTTDYLYNQ-QRAMVAQMQMKIPQATYVESHGVLMGTNWNFEEAERRLKMELVHRAIGANYKQCEALLIKHSWNLQCAMS 808 + RI PV + G ++S THY+L+E P+ S + R P S R+ P P D+N + + + +RRG SD+ + N R M ++ +P AT E VL+G + +EEA RRLK+EL+ R + +C+ LLIK WNL+ A + Sbjct: 229 YGVRIYPVLQNGIKISDTHYWLLEPPR-----SFRMRGTPSLSRYQSERV------PPPSPASQDINTSYINVPSGVTLRRGLSDSNKNVLNTLYREMALRLSQAVPFATEAECQAVLIGCGYKYEEALRRLKLELLCRKGYVSRSRCKRLLIKFDWNLEAATT 381
BLAST of hypothetical protein vs. TrEMBL
Match: A0A183MFG4 (Uncharacterized protein OS=Schistosoma margrebowiei OX=48269 GN=SMRZ_LOCUS14789 PE=4 SV=1) HSP 1 Score: 85.8853 bits (211), Expect = 1.285e-14 Identity = 56/183 (30.60%), Postives = 95/183 (51.91%), Query Frame = 2 Query: 311 LDTPNFNARIEPVAKEGNQVSKTHYFLIEEPKYR--RCNSSKER----------CNPIEMYANSSRLFN----QFCEEPLLPFDMNMHITHEHNSLQVRRGRSDTT-DYLYNQQRAMVAQMQMKIPQATYVESHGVLMGTNWNFEEAERRLKMELVHRAIGANYKQCEALLIKHSWNLQCAMS 808 L +N RI P+ + G ++S THY+L++ P+ + R NS+ R +P+++Y+ N C LP +++RRG+S+ D L N R +V ++ ++PQAT E VL+G+++++EEA RRLK EL+ R + + LL K++W+L A+S Sbjct: 321 LGNSRYNVRIYPIIQNGVKISDTHYWLLDPPRSKTLRDNSTLMRRTTDCFRVPPSSPLDIYSTIDGYTNIESTSLCPPNELPL----------KDVKLRRGKSENCKDSLKNFHRELVLRLMKEVPQATESECQAVLIGSSYDYEEATRRLKFELLCRKGYVSRSHSKRLLHKYNWDLDAAIS 493
BLAST of hypothetical protein vs. Planmine SMEST
Match: SMESG000027619.1 (SMESG000027619.1) HSP 1 Score: 494.197 bits (1271), Expect = 1.662e-178 Identity = 234/234 (100.00%), Postives = 234/234 (100.00%), Query Frame = 2 Query: 131 MGGLLSRRKKPPGQKCKFQNPLPAHEAENVLFMAEEQNNANVYAINSPNRQPRSIRNQTLLDTPNFNARIEPVAKEGNQVSKTHYFLIEEPKYRRCNSSKERCNPIEMYANSSRLFNQFCEEPLLPFDMNMHITHEHNSLQVRRGRSDTTDYLYNQQRAMVAQMQMKIPQATYVESHGVLMGTNWNFEEAERRLKMELVHRAIGANYKQCEALLIKHSWNLQCAMSSLYDHDPN 832 MGGLLSRRKKPPGQKCKFQNPLPAHEAENVLFMAEEQNNANVYAINSPNRQPRSIRNQTLLDTPNFNARIEPVAKEGNQVSKTHYFLIEEPKYRRCNSSKERCNPIEMYANSSRLFNQFCEEPLLPFDMNMHITHEHNSLQVRRGRSDTTDYLYNQQRAMVAQMQMKIPQATYVESHGVLMGTNWNFEEAERRLKMELVHRAIGANYKQCEALLIKHSWNLQCAMSSLYDHDPN Sbjct: 1 MGGLLSRRKKPPGQKCKFQNPLPAHEAENVLFMAEEQNNANVYAINSPNRQPRSIRNQTLLDTPNFNARIEPVAKEGNQVSKTHYFLIEEPKYRRCNSSKERCNPIEMYANSSRLFNQFCEEPLLPFDMNMHITHEHNSLQVRRGRSDTTDYLYNQQRAMVAQMQMKIPQATYVESHGVLMGTNWNFEEAERRLKMELVHRAIGANYKQCEALLIKHSWNLQCAMSSLYDHDPN 234
BLAST of hypothetical protein vs. Planmine SMEST
Match: SMESG000060583.1 (SMESG000060583.1) HSP 1 Score: 102.064 bits (253), Expect = 4.984e-26 Identity = 49/119 (41.18%), Postives = 80/119 (67.23%), Query Frame = 2 Query: 452 MYANSSRLFNQFCEEPLLPFDMNMHITHEHNSLQ-VRRGRSDTT----DYLYNQQRAMVAQMQMKIPQATYVESHGVLMGTNWNFEEAERRLKMELVHRAIGANYKQCEALLIKHSWNL 793 MY N++ + EEP LP+D+N I+ E++ + V+RG+S+ + YL+N R +V +M +KIP AT +E + VL+G NW+F+ AE+RLK++++HR N ++C LL K++WNL Sbjct: 1 MYVNANTFASDQYEEPDLPYDLNEQISEEYSITRPVKRGKSENSKNSKSYLFNMHRGLVVRMMLKIPTATEMECNSVLIGANWDFQGAEKRLKVDMLHRKGYVNQQRCTQLLEKYNWNL 119 The following BLAST results are available for this feature:
BLAST of hypothetical protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of hypothetical protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of hypothetical protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of hypothetical protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of hypothetical protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of hypothetical protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of hypothetical protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of hypothetical protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of hypothetical protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of hypothetical protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of hypothetical protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of hypothetical protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of hypothetical protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of hypothetical protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 2
Analyses
This transcript is derived from or has results from the following analyses Cross References
External references for this transcript
Sequences
The following sequences are available for this feature:
transcript sequence >SMED30023292 ID=SMED30023292|Name=hypothetical protein|organism=Schmidtea mediterranea sexual|type=transcript|length=1036bpback to top protein sequence of SMED30023292-orf-1 >SMED30023292-orf-1 ID=SMED30023292-orf-1|Name=SMED30023292-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=235bp MGGLLSRRKKPPGQKCKFQNPLPAHEAENVLFMAEEQNNANVYAINSPNRback to top Annotated Terms
The following terms have been associated with this transcript:
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