SMED30023288

Overview
NameSMED30023288
Smed IDSMED30023288
Length (bp)1693
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30023288 (SMED30023288) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30023288 (SMED30023288) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30023288 (SMED30023288) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30023288 (SMED30023288) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30023288

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 3

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30023288h1SMcG0010152 SmedASXL_012772SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
neoblastSMED30023288h1SMcG0010152 dd_Smed_v4_14271_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
zeta neoblastSMED30023288h1SMcG0010152 dd_Smed_v4_40776_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of SMED30023288 vs. TrEMBL
Match: A0A4E0QV13 (Uncharacterized protein OS=Fasciola hepatica OX=6192 GN=D915_009290 PE=4 SV=1)

HSP 1 Score: 79.7221 bits (195), Expect = 6.697e-12
Identity = 119/543 (21.92%), Postives = 229/543 (42.17%), Query Frame = 1 
Query:   70 IVMIMKRAFNIDF-NVLNKDHYMEEISNN------FEDDKVLFEDLDGNPLKLQHLVNLN---ESFKNAKDKLSTIFNKSIFNLKVIRPWNIQEYQNYFLRKFITEKEYKSLFLNSICYIDGKFRMRGYAKSDRVNHLKILKIERXXXXXXXXXXXXXXXELLNDPRFKSVYKSVDATYLFPNGEV-SVKWQNCFNV-FDWPDLLADCDLKITIDPFSTNSSLNSILKEIQQLEVIYKAVENDESSWELIDNEKLGLNVDKFKIELEKMLKLCIDKAPCNSDENINIYSWNRNVFTRNKDITEKLWAVFHKINSKAEFKKCVNSMMPILTENYASVEFNPNNTTELCRMLKD---RFLNSEVLITSFM-TNLELIVQVGLHKLTKDLLFIIFNELPDLLQDERNEIYNKC-CKVTQLGTGIESLKQFF--TCV-----------SYMEILKTVLNPQDYKVEALAAVRRFLKSLSSNNSFDLEQSIKLKMRE-INPIIRTLSSFWIVHFECEEPMHCQKAVVFEVENNNAEFHIYRHDMELCD 1605
            ++ ++++ F +   N+++KDH   + S++        +D+   E+  GNPL+L+ +V+L+   + F +   K + +    + NL   RP++ +            E  YK  FL  +   D KF++ G          ++L I  N++    +S K    + +  P   S     +A+Y  P+  V    ++ C +V    P      DL   + P+   S   S+ K+I     ++ A END  SW    +      V++F+  L  + +           E+++    N  +  R  D TE +W     +NS   F+  + S +  L  N   +  + +N T L + L++   +    +V     M + +E ++++GL+KL  D + ++   +PD+        YN        L T   +L   +  TC+           S+++ L+ +LN QD K E     R  L        F L+ S  + + E + PI     +FW++  +   PM     +++E+  +    +   H+M+L D
Sbjct:    1 MISVIRQQFGLSCQNIISKDHDAPQTSDSKTSVYSTAEDEPDSENYMGNPLELEEIVDLSMESKMFAHEVKKCNPV--SRLINLTDRRPFDNKP-----------EGGYKDGFLYHLS--DEKFQLFGLFPLTSSTK-RVLSIHINNEPNCTASIKDLQQKHM--PFSSSFNCGFEASYSLPHCTVFHANFETCESVVLSPPPSFVAGDLVAQLRPYDGESVCPSLFKDI-----MFDAYENDFRSWSSTHDAP---TVEQFQALLPSLFRTAGQSLIDTVSESLSGAKLNTGLVKRFLDPTELIWDTMKNVNSVVHFRSMMKSAVHYLLNNRELIRLSRSNHTRLAQYLRNITAQMGRGQVDPDHMMPSQIECLLEIGLNKLINDCVALLATVIPDVTSLPCLAWYNFADGNGASLTTMWATLHHVYKATCLFLCLSASCSRQSFIDELRKILNQQDPKDEW----RTALYGGPLGEPFVLKHSFSVPVSELVVPISGIPPTFWVMRLDTNHPMRTSVRLLYELLGSQGLRNYVCHEMKLVD 513
[Close]
BLAST of SMED30023288 vs. TrEMBL
Match: A0A5K4F073 (Uncharacterized protein OS=Schistosoma mansoni OX=6183 PE=4 SV=1)

HSP 1 Score: 77.7962 bits (190), Expect = 2.669e-11
Identity = 104/521 (19.96%), Postives = 213/521 (40.88%), Query Frame = 1 
Query:  145 SNNFEDDKVLFEDLDGNPLKLQHLVNLN-ESFKNAKDKLSTIFNKSIFNLKVIRPWNIQEYQNYF--LRKFITEK--EYKSLFLNS-----ICYIDGKFRMRGYAKSDRVNHLKILKIERXXXXXXXXXXXXXXXELLNDPRFKSVYKSVDATYLFPNGEVSVKWQNCFN-VFDWPDLLADCDLKITIDPFSTNSSLNSILKEIQQLEV---IYKAVENDESSWELIDNEKLGLNVDKFKIELEKMLKLCIDKAPCNSDENINIYSWNRNVFTRNKDITEKLWAVFHKINSKAEFKKCVNSMMPILTENYASVEFNPNNTTELCRMLKDRFLNSEVLITS----FMTNLELIVQVGLHKLTKDLLFIIFNELPD---LLQDERNEIYNKCCKVTQLGTGIESLKQFFTCVSYMEILKTVLNPQDYKVEALAAVRRFLKSLSS------------NNSFDLEQSIKLKMRE-INPIIRTLSSFWIVHFECEEPMHCQKAVVFEVENNNAEFHIYRHDMELCD 1605
            S++F +     +D +GNPLK++ + NL+ ES      K S       F+ + ++P   +    Y   +  F+ EK  ++  LF  +     I  I  +F +        VNH               S  ++    +L+ P F   +   +  Y   +G    +++ C + V   P      +L + I P+ +N  ++++L+EIQ L+V   +Y+A E D   W  + N  +  N ++ K E   + K          D      ++N  +  R  D +E +W       +K  F+ CV +    L +    +    +N T+L   L+    ++E    +    F   +E ++++GLHK+  D ++++ + +PD     + +R   +N       L +  + L   +  VS++  L ++++    K   ++ +R+ +   S             N  F +  S  L + E + P+       WI+  + + P+     +++E+   +       H + + D
Sbjct:   37 SDHFTETSSRCDDFEGNPLKIEKIANLSIESSFTDSQKESVKPRSRFFDFRGLKPLENKPLGGYIDNVLYFVREKGEQFIGLFPRTKTSKRILRISSEFNVD-------VNH---------------SVKELSQGSMLSFPEFVCTF---ECMYKLSHGIFRARYETCEDKVLSPPPSFVSGELTVNITPYKSNKLISNLLEEIQILKVKIDLYRAYECDSPVW--LANSVV--NCEQVKAEFPNIFKSVKLNKVDRPDGRACGVNFNHLLSERKLDSSEAIWNAVKGSPNKLTFRTCVENAFEYLIDKSELIHLASHNRTQLAWRLRHLHDSTETYGQNPYGLFNFGIETLIEIGLHKVINDCVYLLDSMIPDSSTFFESDRQSGWN-------LESQWDVLHNLYISVSFLVCLGSMIS----KSSLISELRKIMAQKSQKNEWKSALYEPDNKPFCITHSFSLLVSELVVPVSELPPDLWIMRIDAKNPVPTSIRIIYELVKKDGLEQYACHKLRMID 517
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BLAST of SMED30023288 vs. TrEMBL
Match: A0A074ZDW1 (Uncharacterized protein OS=Opisthorchis viverrini OX=6198 GN=T265_14222 PE=4 SV=1)

HSP 1 Score: 75.485 bits (184), Expect = 1.373e-10
Identity = 89/379 (23.48%), Postives = 165/379 (43.54%), Query Frame = 1 
Query:  535 PRFKSVYKSVDATYLFPNGEVSVKWQNCFNVFDWPDLLADCDLKITIDPFSTNSSLNSILKEIQQLEVIYKAVENDESSWELIDNEKLGLNVDKFKIELEKMLKL-------CIDKAPCNSD-ENINIYSWNRNVFTRNKDITEKLWAVFHKINSKAEFKKCVNSMMPILTENYASVEFNPNNTTELCRMLKDRF-LNSEVLITS---FMTNLELIVQVGLHKLTKDLLFIIFNELPDLLQDERNEI------YNKCC--KVTQLGTGIESLKQFFTCVSYMEILKTVLNPQDYKVEALAAVRRFLKSLSSNNSFDLEQSIKLKMRE-INPIIRTLSSFWIVHFECEEPMHCQKAVVFE-VENNNAEFHIYRHDMELCD 1605
            P F+  Y++V   Y F     + ++  C ++   P       L   +D  S  S L SIL +++ L+ IY+A E   SSW   ++ +  L +++F+  L  +  L        ID+   N+  + ++  S    +  R  D TE LW    +++S   F+  VNS +  L ++   +E +  N T+L   L+        V I S   F   +E ++++GL+KLT D L ++   +P++     +        Y   C   + +    + S     +  +++  L+ +LN  D K E       +   L S+    +    +L + E + PI   L S WI+  + ++P+     +V+E +     E     H+M+L D
Sbjct:  145 PEFRCRYEAV---YQFTCATFTAQFDKCESLIGPPLSFVVGSLSTEVDLLS-GSCLPSILDDLKVLKTIYEAHELGLSSW---NSNQSSLTIEEFQTNLPNIFILFYLVPNKTIDQVKQNTTFDAVSCTSMRGLLANRRPDSTELLWDTVKRVSSGHAFRSFVNSAIAYLLDHREVLELHRRNDTQLAIKLRSLLSRGGPVNIDSGVPFPFGIESLLELGLNKLTNDCLCLLEPFVPEVALSSPDPDKSWSLEYRWACLYHLYKASCFLASFTSHCSKQAFVSELQKILNKPDEKNE-------WRSLLCSDTPLIVRHQFELPVSELLVPISGILPSLWIMQLDADQPVRTSVRLVYEQISGGVGEKKYICHEMKLVD 509
[Close]
BLAST of SMED30023288 vs. TrEMBL
Match: A0A4Z2DBG7 (Uncharacterized protein OS=Schistosoma japonicum OX=6182 GN=EWB00_002693 PE=4 SV=1)

HSP 1 Score: 74.7146 bits (182), Expect = 2.043e-10
Identity = 86/384 (22.40%), Postives = 168/384 (43.75%), Query Frame = 1 
Query:  523 LLNDPRFKSVYKSVDATYLFPNGEVSVKWQNCFN-VFDWPDLLADCDLKITIDPFSTNSSLNSILKEIQQLEVIYKAVENDESSW---ELIDNEKLGLNVDKF--KIELEKMLKLCIDKAPCNSDENINIYSWNRNVFTRNK-DITEKLWAVFHKINSKAEFKKCVNSMMPILTENYASVEFNPNNTTELC---RMLKDRFLNSEVLITSFMTNLELIVQVGLHKLTKDLLFIIFNELPDLLQDERNEIYNKCCKVTQLGTGIES----LKQFFTCVSYMEILKTV-----LNPQDYKVEALAAVRRFLKSL---SSNNSFDLEQSIKLKMRE-INPIIRTLSSFWIVHFECEEPMHCQKAVVFEVENNNAEFHIYRHDMELCD 1605
            +L+ P F   +   +  Y   +G    K++ C + +   P      +L + I P+ +N  L SIL+EIQ L+ +Y+A E D  +W    +ID E++          +++ K+ +  +D   C S+ ++        + +R K D +E +W V     +K  F+ C+      L +    +    +N T+L    R L D    S     S+   +E ++++GLHKL  D ++++ + +P+      N+         ++G+ +ES    L   +  VS++  L +V     L P+  K+ A  + +   KS      N  F +  S  L + E I P+ +     WI+  + + P+     +++E+   +       H + + D
Sbjct:  151 MLSFPDFVCTF---ECMYKLSHGVFDAKFETCEDKILSPPPSFVSGELTVNIMPYKSNELLPSILEEIQILKDLYRACECDSPAWLPNSVIDCEQVKAEFPNIFKSVKVNKVDR--VDGKVCGSNFSL--------LLSRRKLDSSEVIWNVVKGSPNKFTFRTCIEDAFEYLMDKSELIYLANHNQTQLAWRLRHLHDSTKPSGQ--HSYGLGIETLIEIGLHKLMNDCIYLLESIIPESSSFFEND--------KEIGSSLESRWETLHNLYKYVSFLACLASVASKSSLVPELRKILAQKSQKNEWKSALYEPDNIPFCMTHSFSLLVSELIVPLSQLPPDLWIMRIDAKNPVPTSIRIIYELVTKDGLEQYICHKLRMID 511
[Close]
BLAST of SMED30023288 vs. TrEMBL
Match: A0A5J4NVX9 (Uncharacterized protein OS=Paragonimus westermani OX=34504 GN=DEA37_0014468 PE=4 SV=1)

HSP 1 Score: 67.781 bits (164), Expect = 2.010e-8
Identity = 77/327 (23.55%), Postives = 150/327 (45.87%), Query Frame = 1 
Query:  688 TNSSLNSILKEIQQLEV-----IYKAVENDESSWELIDNEKLGLNVDKFKIELEKMLKLCIDKAPCNSDENINIYSWNRNVFT-RNKDITEKLWAVFHKINSKAEFKKCVNSMMPILTENYASVEFNPNNTTELCRMLKDRFLNSEVLI-----TSFMTNLELIVQVGLHKLTKDLLFIIFNELPDLLQ---DERN--EIYNKCCKVTQLGTG---IESLKQFFTCVSYMEILKTVLNPQDYKVEALAAVRRFLKSLSSNNSFDLEQSIKLKMRE-INPIIRTLSSFWIVHFECEEPMHCQKAVVFEVENNNAEFHIYR-HDMELCD 1605
            T+  L + +KE+Q++ +     I+ A E+D S W+  +     L ++ F+  L  + K   D   C++  +  I    R+V   R+ D TE+LW     ++S+  F+  +N     L ++   ++ + +N T+L   L+   LNS+  I     ++F   +E ++++GL+KLT D + ++   +PD +    DE +  E++++  ++  L        SL  + +    +  ++ +LN  D K E     R  L    S     +   + L + E + P+     S WI+  +  EP+     +V+E      +   Y  H+M L D
Sbjct:   53 TDPDLCTSIKEVQRVHMAMFSSIFTAYESDCSVWQTAEEP---LTLEMFQSSLPLLFK---DTQICDTIPSGGI----RSVPPFRHLDSTERLWDAIKIVHSRQTFRGLLNCATHYLLKHRELLKLHRHNHTQLATRLRT-LLNSQGPIHLDSSSAFPYGIEYLLEIGLNKLTNDCISVLETAIPDCVSTVDDEASVVELHHRWAQLHHLYKASCLFLSLSPYCSKQVVIREVRAILNTSDPKGEW----RVGLFEDPSRGPLVVSHQLSLPLSELLVPLSSIPPSLWIMRMDATEPVRTSVRLVYEHVPTRDDVDRYICHEMRLTD 364
[Close]
BLAST of SMED30023288 vs. Planmine SMEST
Match: SMESG000064764.1 (SMESG000064764.1)

HSP 1 Score: 1045.03 bits (2701), Expect = 0.000e+0
Identity = 530/533 (99.44%), Postives = 533/533 (100.00%), Query Frame = 1 
Query:   28 MADFSELYSNEVKNIVMIMKRAFNIDFNVLNKDHYMEEISNNFEDDKVLFEDLDGNPLKLQHLVNLNESFKNAKDKLSTIFNKSIFNLKVIRPWNIQEYQNYFLRKFITEKEYKSLFLNSICYIDGKFRMRGYAKSDRVNHLKILKIERXXXXXXXXXXXXXXXELLNDPRFKSVYKSVDATYLFPNGEVSVKWQNCFNVFDWPDLLADCDLKITIDPFSTNSSLNSILKEIQQLEVIYKAVENDESSWELIDNEKLGLNVDKFKIELEKMLKLCIDKAPCNSDENINIYSWNRNVFTRNKDITEKLWAVFHKINSKAEFKKCVNSMMPILTENYASVEFNPNNTTELCRMLKDRFLNSEVLITSFMTNLELIVQVGLHKLTKDLLFIIFNELPDLLQDERNEIYNKCCKVTQLGTGIESLKQFFTCVSYMEILKTVLNPQDYKVEALAAVRRFLKSLSSNNSFDLEQSIKLKMREINPIIRTLSSFWIVHFECEEPMHCQKAVVFEVENNNAEFHIYRHDMELCDMPMFSRN 1626
            MADFSELYSNEVKNIVMIMKRAFNIDFNVLNKDHYMEEISNNFEDDKVLFEDLDGNPLKLQHLVNLNESFKNAKDKLSTIFNKSIFNLKVIRPWNIQEYQNYFLRKFITEKEYKSLFLNSICYIDGKFRMRG+AKSDRVNHLKILKIERNSKIILKSSNKIKISELLNDPRFKSVYKSVDATYLFPNGEVSVKWQNCFNVFDWPDLLADCDLKITIDPFSTNSSLNSILKEIQQLEVIYKAVENDESSWELID+EKLGLNVDKFKIELEKMLKLCIDKAPCNSDENINIYSWNRNVFTRNKDITEKLWA+FHKINSKAEFKKCVNSMMPILTENYASVEFNPNNTTELCRMLKDRFLNSEVLITSFMTNLELIVQVGLHKLTKDLLFIIFNELPDLLQDERNEIYNKCCKVTQLGTGIESLKQFFTCVSYMEILKTVLNPQDYKVEALAAVRRFLKSLSSNNSFDLEQSIKLKMREINPIIRTLSSFWIVHFECEEPMHCQKAVVFEVENNNAEFHIYRHDMELCDMPMFSRN
Sbjct:    1 MADFSELYSNEVKNIVMIMKRAFNIDFNVLNKDHYMEEISNNFEDDKVLFEDLDGNPLKLQHLVNLNESFKNAKDKLSTIFNKSIFNLKVIRPWNIQEYQNYFLRKFITEKEYKSLFLNSICYIDGKFRMRGFAKSDRVNHLKILKIERNSKIILKSSNKIKISELLNDPRFKSVYKSVDATYLFPNGEVSVKWQNCFNVFDWPDLLADCDLKITIDPFSTNSSLNSILKEIQQLEVIYKAVENDESSWELIDDEKLGLNVDKFKIELEKMLKLCIDKAPCNSDENINIYSWNRNVFTRNKDITEKLWALFHKINSKAEFKKCVNSMMPILTENYASVEFNPNNTTELCRMLKDRFLNSEVLITSFMTNLELIVQVGLHKLTKDLLFIIFNELPDLLQDERNEIYNKCCKVTQLGTGIESLKQFFTCVSYMEILKTVLNPQDYKVEALAAVRRFLKSLSSNNSFDLEQSIKLKMREINPIIRTLSSFWIVHFECEEPMHCQKAVVFEVENNNAEFHIYRHDMELCDMPMFSRN 533
The following BLAST results are available for this feature:
BLAST of SMED30023288 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023288 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023288 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023288 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023288 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023288 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023288 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023288 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A4E0QV136.697e-1221.92Uncharacterized protein OS=Fasciola hepatica OX=61... [more]
A0A5K4F0732.669e-1119.96Uncharacterized protein OS=Schistosoma mansoni OX=... [more]
A0A074ZDW11.373e-1023.48Uncharacterized protein OS=Opisthorchis viverrini ... [more]
A0A4Z2DBG72.043e-1022.40Uncharacterized protein OS=Schistosoma japonicum O... [more]
A0A5J4NVX92.010e-823.55Uncharacterized protein OS=Paragonimus westermani ... [more]
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BLAST of SMED30023288 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023288 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023288 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023288 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023288 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023288 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000064764.10.000e+099.44SMESG000064764.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30023288 ID=SMED30023288|Name=SMED30023288|organism=Schmidtea mediterranea sexual|type=transcript|length=1693bp
CATTTCTATTCATAAATATATATTCTAATGGCTGACTTTTCTGAATTATA
CAGTAATGAGGTGAAAAATATTGTGATGATAATGAAGCGAGCTTTTAATA
TTGATTTTAACGTTTTAAATAAAGATCATTATATGGAGGAAATTTCAAAC
AACTTTGAAGATGATAAAGTACTATTTGAAGATCTCGATGGAAATCCATT
AAAATTGCAACATTTAGTTAATCTAAATGAAAGTTTTAAAAATGCCAAGG
ATAAACTTTCGACTATTTTTAATAAAAGTATTTTTAATTTAAAAGTTATT
AGACCATGGAATATACAAGAATATCAAAACTATTTTCTCCGTAAGTTTAT
TACAGAAAAAGAATACAAAAGTTTATTCTTAAACAGTATTTGTTATATTG
ATGGTAAATTTCGGATGCGTGGATATGCAAAATCCGATAGAGTAAATCAT
TTAAAAATTCTTAAAATAGAAAGAAATTCTAAAATAATATTAAAATCATC
AAATAAAATTAAAATATCCGAACTGTTAAATGATCCCAGATTTAAGAGCG
TATATAAAAGTGTGGATGCTACATATTTGTTTCCAAATGGAGAAGTGTCA
GTAAAATGGCAAAATTGTTTCAATGTTTTTGATTGGCCAGATTTATTGGC
GGATTGTGATTTGAAAATAACCATTGATCCATTTTCTACAAATAGTAGCC
TTAATTCAATTCTTAAAGAAATACAGCAACTAGAAGTCATTTATAAAGCA
GTAGAAAACGACGAGAGTTCTTGGGAATTAATTGATAATGAGAAACTTGG
ATTAAATGTTGATAAATTTAAAATTGAATTGGAAAAAATGCTGAAACTGT
GTATCGATAAAGCTCCATGCAACTCTGATGAGAATATTAACATCTATTCA
TGGAATCGAAATGTATTTACAAGAAATAAAGATATCACTGAGAAACTGTG
GGCGGTTTTTCACAAAATCAATAGCAAAGCTGAATTTAAAAAGTGTGTTA
ATTCAATGATGCCAATTCTTACTGAAAATTATGCCAGTGTTGAGTTCAAT
CCAAACAATACAACAGAGTTATGTCGCATGCTAAAAGACAGGTTTTTGAA
TTCTGAAGTATTGATTACGTCGTTCATGACAAATTTGGAATTGATTGTAC
AAGTTGGATTACATAAATTGACCAAGGATTTATTGTTCATTATTTTCAAT
GAGTTGCCCGATTTATTGCAAGATGAAAGAAATGAAATCTATAACAAATG
TTGCAAAGTTACTCAATTAGGAACTGGTATTGAATCTTTGAAACAGTTTT
TCACGTGTGTTTCATACATGGAAATCCTAAAAACTGTTCTCAATCCGCAA
GATTATAAAGTTGAAGCTTTGGCAGCTGTTAGGAGGTTTTTAAAATCGTT
ATCTTCCAATAATTCTTTTGATTTGGAACAATCAATAAAGCTTAAGATGC
GAGAAATTAACCCTATAATTAGGACTCTAAGTTCATTCTGGATTGTGCAT
TTTGAATGCGAAGAACCTATGCACTGTCAAAAAGCAGTTGTTTTCGAAGT
TGAGAATAATAATGCGGAATTCCACATATACCGTCATGATATGGAACTTT
GTGACATGCCAATGTTTAGCAGGAATTAATGGAATTTTCAAATAGATGAG
TCTATGTTGTGTAAATTGTGTTGAAATGTTTGTTGAACATAAC
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protein sequence of SMED30023288-orf-1

>SMED30023288-orf-1 ID=SMED30023288-orf-1|Name=SMED30023288-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=534bp
MADFSELYSNEVKNIVMIMKRAFNIDFNVLNKDHYMEEISNNFEDDKVLF
EDLDGNPLKLQHLVNLNESFKNAKDKLSTIFNKSIFNLKVIRPWNIQEYQ
NYFLRKFITEKEYKSLFLNSICYIDGKFRMRGYAKSDRVNHLKILKIERN
SKIILKSSNKIKISELLNDPRFKSVYKSVDATYLFPNGEVSVKWQNCFNV
FDWPDLLADCDLKITIDPFSTNSSLNSILKEIQQLEVIYKAVENDESSWE
LIDNEKLGLNVDKFKIELEKMLKLCIDKAPCNSDENINIYSWNRNVFTRN
KDITEKLWAVFHKINSKAEFKKCVNSMMPILTENYASVEFNPNNTTELCR
MLKDRFLNSEVLITSFMTNLELIVQVGLHKLTKDLLFIIFNELPDLLQDE
RNEIYNKCCKVTQLGTGIESLKQFFTCVSYMEILKTVLNPQDYKVEALAA
VRRFLKSLSSNNSFDLEQSIKLKMREINPIIRTLSSFWIVHFECEEPMHC
QKAVVFEVENNNAEFHIYRHDMELCDMPMFSRN*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000014zeta neoblast
PLANA:0000429neoblast
PLANA:0002109X1 cell
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 225..245