SMED30023281

Overview


Name	SMED30023281
Smed ID	SMED30023281
Length (bp)	500

Neoblast Clusters

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.

Expression of SMED30023281 (SMED30023281) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30023281 (SMED30023281) in cells (dots) of each of the 12 neoblast clusters.

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30023281 (SMED30023281) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30023281 (SMED30023281) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30023281

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 4

Expressed In	Reference Transcript	Gene Models	Published Transcript	Transcriptome	Publication
X1 cell	SMED30023281	SMESG000006493.1	SmedASXL_006714	SmedAsxl_ww_GCZZ01	PMID:26114597 Zhu et al., 2015
epidermis	SMED30023281	SMESG000006493.1 SMESG000006492.1 SMESG000006491.1	dd_Smed_v4_2424_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
neoblast	SMED30023281	SMESG000006493.1 SMESG000006492.1 SMESG000006491.1	dd_Smed_v4_2424_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
muscle cell	SMED30023281	SMESG000006493.1 SMESG000006492.1 SMESG000006491.1	dd_Smed_v4_2424_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018

Note: Hover over icons to view figure legend

Alignments

SMED30023281 aligns in the following genomic locations:


Alignment Location	Alignment Score
v31.000119:154300..154873 +	2389

Homology

[Close]

BLAST of SMED30023281 vs. Ensembl Human
Match: SRSF3 (serine and arginine rich splicing factor 3 [Source:HGNC Symbol;Acc:HGNC:10785])

HSP 1 Score: 106.686 bits (265), Expect = 1.026e-29
Identity = 48/80 (60.00%), Postives = 63/80 (78.75%), Query Frame = -1

Query:  186 EVKVYVGDLPKDANEKEIEKAFKDFGPIKSVWVARNPAGFAFVMFDDYRDAEDAVKELDGSVICGVRVRVEMSHGRSRPK 425
            + KVYVG+L  + N+ E+E+AF  +GP++SVWVARNP GFAFV F+D RDA DAV+ELDG  +CG RVRVE+S+G  R +
Sbjct:    9 DCKVYVGNLGNNGNKTELERAFGYYGPLRSVWVARNPPGFAFVEFEDPRDAADAVRELDGRTLCGCRVRVELSNGEKRSR 88

[Close]

BLAST of SMED30023281 vs. Ensembl Human
Match: SRSF7 (serine and arginine rich splicing factor 7 [Source:HGNC Symbol;Acc:HGNC:10789])

HSP 1 Score: 106.301 bits (264), Expect = 1.823e-29
Identity = 49/78 (62.82%), Postives = 61/78 (78.21%), Query Frame = -1

Query:  192 EVKVYVGDLPKDANEKEIEKAFKDFGPIKSVWVARNPAGFAFVMFDDYRDAEDAVKELDGSVICGVRVRVEMSHGRSR 425
            E KVYVG+L   A + E+E+AF  +GP+++VW+ARNP GFAFV F+D RDAEDAV+ LDG VICG RVRVE+S G  R
Sbjct:   10 ETKVYVGNLGTGAGKGELERAFSYYGPLRTVWIARNPPGFAFVEFEDPRDAEDAVRGLDGKVICGSRVRVELSTGMPR 87

[Close]

Query:  192 EVKVYVGDLPKDANEKEIEKAFKDFGPIKSVWVARNPAGFAFVMFDDYRDAEDAVKELDGSVICGVRVRVEMSHGRSR 425
            E KVYVG+L   A + E+E+AF  +GP+++VW+ARNP GFAFV F+D RDAEDAV+ LDG VICG RVRVE+S G  R
Sbjct:   10 ETKVYVGNLGTGAGKGELERAFSYYGPLRTVWIARNPPGFAFVEFEDPRDAEDAVRGLDGKVICGSRVRVELSTGMPR 87

[Close]

BLAST of SMED30023281 vs. Ensembl Human
Match: SRSF7 (serine and arginine rich splicing factor 7 [Source:HGNC Symbol;Acc:HGNC:10789])

HSP 1 Score: 106.301 bits (264), Expect = 2.077e-29
Identity = 49/78 (62.82%), Postives = 61/78 (78.21%), Query Frame = -1

Query:  192 EVKVYVGDLPKDANEKEIEKAFKDFGPIKSVWVARNPAGFAFVMFDDYRDAEDAVKELDGSVICGVRVRVEMSHGRSR 425
            E KVYVG+L   A + E+E+AF  +GP+++VW+ARNP GFAFV F+D RDAEDAV+ LDG VICG RVRVE+S G  R
Sbjct:   10 ETKVYVGNLGTGAGKGELERAFSYYGPLRTVWIARNPPGFAFVEFEDPRDAEDAVRGLDGKVICGSRVRVELSTGMPR 87

[Close]

BLAST of SMED30023281 vs. Ensembl Human
Match: SRSF7 (serine and arginine rich splicing factor 7 [Source:HGNC Symbol;Acc:HGNC:10789])

HSP 1 Score: 106.301 bits (264), Expect = 2.077e-29
Identity = 49/78 (62.82%), Postives = 61/78 (78.21%), Query Frame = -1

Query:  192 EVKVYVGDLPKDANEKEIEKAFKDFGPIKSVWVARNPAGFAFVMFDDYRDAEDAVKELDGSVICGVRVRVEMSHGRSR 425
            E KVYVG+L   A + E+E+AF  +GP+++VW+ARNP GFAFV F+D RDAEDAV+ LDG VICG RVRVE+S G  R
Sbjct:   10 ETKVYVGNLGTGAGKGELERAFSYYGPLRTVWIARNPPGFAFVEFEDPRDAEDAVRGLDGKVICGSRVRVELSTGMPR 87

[Close]

BLAST of SMED30023281 vs. Ensembl Celegans
Match: rsp-6 (Probable splicing factor, arginine/serine-rich 6 [Source:UniProtKB/Swiss-Prot;Acc:Q18409])

HSP 1 Score: 102.064 bits (253), Expect = 1.213e-27
Identity = 48/73 (65.75%), Postives = 56/73 (76.71%), Query Frame = -1

Query:  207 EVKVYVGDLPKDANEKEIEKAFKDFGPIKSVWVARNPAGFAFVMFDDYRDAEDAVKELDGSVICGVRVRVEMS 425
            + KVYVG LP DA  +E+E+ F  FG I+ VWVAR P GFAFV +DD RDAEDAV+ LDGS ICGVR RVE+S
Sbjct:    2 DAKVYVGGLPSDATSQELEEIFDRFGRIRKVWVARRPPGFAFVEYDDVRDAEDAVRALDGSRICGVRARVELS 74

[Close]

BLAST of SMED30023281 vs. Ensembl Celegans
Match: Y57G11A.5 (pep chromosome:WBcel235:IV:14511884:14512505:-1 gene:WBGene00013293.1 transcript:Y57G11A.5.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:Y57G11A.5)

HSP 1 Score: 82.4185 bits (202), Expect = 8.215e-21
Identity = 40/76 (52.63%), Postives = 51/76 (67.11%), Query Frame = -1

Query:  210 KNKDEVKVYVGDLPKDANEKEIEKAFKDFGPIKSVWVARNPAGFAFVMFDDYRDAEDAVKELDGSVICGVRVRVEM 437
            +N +  +VYVG++P DA EKEIE  F   GPIKS+W+A+ P GFAFV F     A DAVK L+G  IC +  +VEM
Sbjct:   12 RNVENRQVYVGNMPFDATEKEIEAVFSVMGPIKSIWMAKRPPGFAFVTFKRTVHAFDAVKYLNGKKICDLEAKVEM 87

[Close]

BLAST of SMED30023281 vs. Ensembl Celegans
Match: rsp-3 (Probable splicing factor, arginine/serine-rich 3 [Source:UniProtKB/Swiss-Prot;Acc:Q9NEW6])

HSP 1 Score: 71.2478 bits (173), Expect = 2.704e-15
Identity = 40/73 (54.79%), Postives = 49/73 (67.12%), Query Frame = -1

Query:  207 KVYVGDLPKDANEKEIEKAFKDFGPIKSVWV--ARNPAGFAFVMFDDYRDAEDAVKELDGSVICGVRVRVEMS 419
            KVYVG+LP D  EKE+E  F  +G IK V +   R PA FAFV F+D+RDAEDAV+  DG    G R+RVE +
Sbjct:   10 KVYVGNLPGDVREKEVEDIFHKYGRIKYVDIKSGRGPA-FAFVEFEDHRDAEDAVRARDGYEFDGRRIRVEFT 81

[Close]

Query:  207 KVYVGDLPKDANEKEIEKAFKDFGPIKSVWV--ARNPAGFAFVMFDDYRDAEDAVKELDGSVICGVRVRVEMS 419
            KVYVG+LP D  EKE+E  F  +G IK V +   R PA FAFV F+D+RDAEDAV+  DG    G R+RVE +
Sbjct:   10 KVYVGNLPGDVREKEVEDIFHKYGRIKYVDIKSGRGPA-FAFVEFEDHRDAEDAVRARDGYEFDGRRIRVEFT 81

[Close]

Query:  207 KVYVGDLPKDANEKEIEKAFKDFGPIKSVWV--ARNPAGFAFVMFDDYRDAEDAVKELDGSVICGVRVRVEMS 419
            KVYVG+LP D  EKE+E  F  +G IK V +   R PA FAFV F+D+RDAEDAV+  DG    G R+RVE +
Sbjct:   10 KVYVGNLPGDVREKEVEDIFHKYGRIKYVDIKSGRGPA-FAFVEFEDHRDAEDAVRARDGYEFDGRRIRVEFT 81

[Close]

BLAST of SMED30023281 vs. Ensembl Fly
Match: Rbp1 (gene:FBgn0260944 transcript:FBtr0082277)

HSP 1 Score: 99.7525 bits (247), Expect = 5.131e-27
Identity = 44/70 (62.86%), Postives = 55/70 (78.57%), Query Frame = -1

Query:  210 KVYVGDLPKDANEKEIEKAFKDFGPIKSVWVARNPAGFAFVMFDDYRDAEDAVKELDGSVICGVRVRVEM 419
            KVYVG+L   A++ EIE AF  +GP+++VWVARNP GFAFV F+D RDAEDA + LDG+  CG R+RVEM
Sbjct:   12 KVYVGNLGSSASKHEIEGAFAKYGPLRNVWVARNPPGFAFVEFEDRRDAEDATRALDGTRCCGTRIRVEM 81

[Close]

BLAST of SMED30023281 vs. Ensembl Fly
Match: Rbp1 (gene:FBgn0260944 transcript:FBtr0082275)

HSP 1 Score: 97.8265 bits (242), Expect = 2.265e-26
Identity = 44/70 (62.86%), Postives = 55/70 (78.57%), Query Frame = -1

Query:  210 KVYVGDLPKDANEKEIEKAFKDFGPIKSVWVARNPAGFAFVMFDDYRDAEDAVKELDGSVICGVRVRVEM 419
            KVYVG+L   A++ EIE AF  +GP+++VWVARNP GFAFV F+D RDAEDA + LDG+  CG R+RVEM
Sbjct:   12 KVYVGNLGSSASKHEIEGAFAKYGPLRNVWVARNPPGFAFVEFEDRRDAEDATRALDGTRCCGTRIRVEM 81

[Close]

BLAST of SMED30023281 vs. Ensembl Fly
Match: Rbp1-like (gene:FBgn0030479 transcript:FBtr0339838)

HSP 1 Score: 96.6709 bits (239), Expect = 9.514e-26
Identity = 44/70 (62.86%), Postives = 55/70 (78.57%), Query Frame = -1

Query:  210 KVYVGDLPKDANEKEIEKAFKDFGPIKSVWVARNPAGFAFVMFDDYRDAEDAVKELDGSVICGVRVRVEM 419
            KVYVG+L   A++ EIE AF  +GP+++VWVARNP GFAFV F+D RDAEDA + LDG+  CG R+RVEM
Sbjct:   12 KVYVGNLGSSASKYEIENAFSKYGPLRNVWVARNPPGFAFVEFEDRRDAEDATRGLDGTRCCGTRIRVEM 81

[Close]

BLAST of SMED30023281 vs. Ensembl Fly
Match: Rbp1-like (gene:FBgn0030479 transcript:FBtr0073790)

HSP 1 Score: 96.6709 bits (239), Expect = 9.514e-26
Identity = 44/70 (62.86%), Postives = 55/70 (78.57%), Query Frame = -1

Query:  210 KVYVGDLPKDANEKEIEKAFKDFGPIKSVWVARNPAGFAFVMFDDYRDAEDAVKELDGSVICGVRVRVEM 419
            KVYVG+L   A++ EIE AF  +GP+++VWVARNP GFAFV F+D RDAEDA + LDG+  CG R+RVEM
Sbjct:   12 KVYVGNLGSSASKYEIENAFSKYGPLRNVWVARNPPGFAFVEFEDRRDAEDATRGLDGTRCCGTRIRVEM 81

[Close]

BLAST of SMED30023281 vs. Ensembl Fly
Match: Rbp1-like (gene:FBgn0030479 transcript:FBtr0304001)

HSP 1 Score: 97.0561 bits (240), Expect = 5.918e-25
Identity = 44/71 (61.97%), Postives = 55/71 (77.46%), Query Frame = -1

Query:  210 VKVYVGDLPKDANEKEIEKAFKDFGPIKSVWVARNPAGFAFVMFDDYRDAEDAVKELDGSVICGVRVRVEM 422
             KVYVG+L   A++ EIE AF  +GP+++VWVARNP GFAFV F+D RDAEDA + LDG+  CG R+RVEM
Sbjct:   11 CKVYVGNLGSSASKYEIENAFSKYGPLRNVWVARNPPGFAFVEFEDRRDAEDATRGLDGTRCCGTRIRVEM 81

[Close]

BLAST of SMED30023281 vs. Ensembl Zebrafish
Match: srsf7a (serine and arginine rich splicing factor 7a [Source:ZFIN;Acc:ZDB-GENE-040426-1798])

HSP 1 Score: 110.923 bits (276), Expect = 7.644e-31
Identity = 52/87 (59.77%), Postives = 66/87 (75.86%), Query Frame = -1

Query:  165 EVKVYVGDLPKDANEKEIEKAFKDFGPIKSVWVARNPAGFAFVMFDDYRDAEDAVKELDGSVICGVRVRVEMSHGRSRPKPWANQSR 425
            + KVYVGDL   A + E+E+AF  +GP++SVWVARNP GFAFV ++D RDAEDAVK +DG V+CG RVRVE+S+G SR   +   SR
Sbjct:   13 DCKVYVGDLGNGAAKGELERAFSYYGPLRSVWVARNPPGFAFVEYEDARDAEDAVKGMDGKVLCGARVRVELSNGMSRKSRYGRPSR 99

[Close]

BLAST of SMED30023281 vs. Ensembl Zebrafish
Match: srsf7a (serine and arginine rich splicing factor 7a [Source:ZFIN;Acc:ZDB-GENE-040426-1798])

HSP 1 Score: 110.538 bits (275), Expect = 7.913e-31
Identity = 52/87 (59.77%), Postives = 66/87 (75.86%), Query Frame = -1

Query:  165 EVKVYVGDLPKDANEKEIEKAFKDFGPIKSVWVARNPAGFAFVMFDDYRDAEDAVKELDGSVICGVRVRVEMSHGRSRPKPWANQSR 425
            + KVYVGDL   A + E+E+AF  +GP++SVWVARNP GFAFV ++D RDAEDAVK +DG V+CG RVRVE+S+G SR   +   SR
Sbjct:   13 DCKVYVGDLGNGAAKGELERAFSYYGPLRSVWVARNPPGFAFVEYEDARDAEDAVKGMDGKVLCGARVRVELSNGMSRKSRYGRPSR 99

[Close]

BLAST of SMED30023281 vs. Ensembl Zebrafish
Match: srsf7a (serine and arginine rich splicing factor 7a [Source:ZFIN;Acc:ZDB-GENE-040426-1798])

HSP 1 Score: 107.842 bits (268), Expect = 1.082e-30
Identity = 50/78 (64.10%), Postives = 63/78 (80.77%), Query Frame = -1

Query:  192 EVKVYVGDLPKDANEKEIEKAFKDFGPIKSVWVARNPAGFAFVMFDDYRDAEDAVKELDGSVICGVRVRVEMSHGRSR 425
            + KVYVGDL   A + E+E+AF  +GP++SVWVARNP GFAFV ++D RDAEDAVK +DG V+CG RVRVE+S+G SR
Sbjct:   13 DCKVYVGDLGNGAAKGELERAFSYYGPLRSVWVARNPPGFAFVEYEDARDAEDAVKGMDGKVLCGARVRVELSNGMSR 90

[Close]

BLAST of SMED30023281 vs. Ensembl Zebrafish
Match: srsf7b (serine and arginine rich splicing factor 7b [Source:ZFIN;Acc:ZDB-GENE-050913-95])

HSP 1 Score: 109.383 bits (272), Expect = 2.207e-30
Identity = 50/84 (59.52%), Postives = 64/84 (76.19%), Query Frame = -1

Query:  192 MSKNKDEVKVYVGDLPKDANEKEIEKAFKDFGPIKSVWVARNPAGFAFVMFDDYRDAEDAVKELDGSVICGVRVRVEMSHGRSR 443
              ++  E KVYVG+L   A + E+E+AF  +GP++SVW+ARNPAGFAFV F+D RDAED+V+ LDG VICG RVRVE+S G  R
Sbjct:    4 FGRHGGETKVYVGNLGTGAGKGELERAFGYYGPLRSVWIARNPAGFAFVEFEDPRDAEDSVRGLDGKVICGSRVRVELSTGMPR 87

[Close]

BLAST of SMED30023281 vs. Ensembl Zebrafish
Match: srsf7b (serine and arginine rich splicing factor 7b [Source:ZFIN;Acc:ZDB-GENE-050913-95])

HSP 1 Score: 108.612 bits (270), Expect = 2.249e-30
Identity = 50/84 (59.52%), Postives = 64/84 (76.19%), Query Frame = -1

Query:  192 MSKNKDEVKVYVGDLPKDANEKEIEKAFKDFGPIKSVWVARNPAGFAFVMFDDYRDAEDAVKELDGSVICGVRVRVEMSHGRSR 443
              ++  E KVYVG+L   A + E+E+AF  +GP++SVW+ARNPAGFAFV F+D RDAED+V+ LDG VICG RVRVE+S G  R
Sbjct:    4 FGRHGGETKVYVGNLGTGAGKGELERAFGYYGPLRSVWIARNPAGFAFVEFEDPRDAEDSVRGLDGKVICGSRVRVELSTGMPR 87

[Close]

BLAST of SMED30023281 vs. Ensembl Xenopus
Match: phex (phosphate regulating endopeptidase homolog X-linked [Source:Xenbase;Acc:XB-GENE-1012247])

HSP 1 Score: 107.071 bits (266), Expect = 1.044e-29
Identity = 48/80 (60.00%), Postives = 63/80 (78.75%), Query Frame = -1

Query:  186 EVKVYVGDLPKDANEKEIEKAFKDFGPIKSVWVARNPAGFAFVMFDDYRDAEDAVKELDGSVICGVRVRVEMSHGRSRPK 425
            + KVYVG+L  + N+ E+E+AF  +GP++SVWVARNP GFAFV F+D RDA DAV+ELDG  +CG RVRVE+S+G  R +
Sbjct:    9 DCKVYVGNLGNNGNKTELERAFGYYGPLRSVWVARNPPGFAFVEFEDPRDAADAVRELDGRTLCGCRVRVELSNGEKRSR 88

[Close]

BLAST of SMED30023281 vs. Ensembl Xenopus
Match: phex (phosphate regulating endopeptidase homolog X-linked [Source:Xenbase;Acc:XB-GENE-1012247])

HSP 1 Score: 106.686 bits (265), Expect = 2.801e-29
Identity = 48/78 (61.54%), Postives = 62/78 (79.49%), Query Frame = -1

Query:  192 EVKVYVGDLPKDANEKEIEKAFKDFGPIKSVWVARNPAGFAFVMFDDYRDAEDAVKELDGSVICGVRVRVEMSHGRSR 425
            + KVYVG+L  + N+ E+E+AF  +GP++SVWVARNP GFAFV F+D RDA DAV+ELDG  +CG RVRVE+S+G  R
Sbjct:    9 DCKVYVGNLGNNGNKTELERAFGYYGPLRSVWVARNPPGFAFVEFEDPRDAADAVRELDGRTLCGCRVRVELSNGEKR 86

[Close]

BLAST of SMED30023281 vs. Ensembl Xenopus
Match: sms (spermine synthase [Source:Xenbase;Acc:XB-GENE-953534])

HSP 1 Score: 107.457 bits (267), Expect = 5.317e-29
Identity = 49/78 (62.82%), Postives = 61/78 (78.21%), Query Frame = -1

Query:  192 EVKVYVGDLPKDANEKEIEKAFKDFGPIKSVWVARNPAGFAFVMFDDYRDAEDAVKELDGSVICGVRVRVEMSHGRSR 425
            E KVYVG+L   A + E+E+AF  +GP+++VW+ARNP GFAFV F+D RDAEDAV+ LDG VICG RVRVE+S G  R
Sbjct:   10 EAKVYVGNLGTGAGKGELERAFSYYGPLRTVWIARNPPGFAFVEFEDTRDAEDAVRGLDGKVICGSRVRVELSTGMPR 87

[Close]

BLAST of SMED30023281 vs. Ensembl Xenopus
Match: sms (spermine synthase [Source:Xenbase;Acc:XB-GENE-953534])

HSP 1 Score: 107.457 bits (267), Expect = 7.312e-29
Identity = 49/78 (62.82%), Postives = 61/78 (78.21%), Query Frame = -1

Query:  192 EVKVYVGDLPKDANEKEIEKAFKDFGPIKSVWVARNPAGFAFVMFDDYRDAEDAVKELDGSVICGVRVRVEMSHGRSR 425
            E KVYVG+L   A + E+E+AF  +GP+++VW+ARNP GFAFV F+D RDAEDAV+ LDG VICG RVRVE+S G  R
Sbjct:   10 EAKVYVGNLGTGAGKGELERAFSYYGPLRTVWIARNPPGFAFVEFEDTRDAEDAVRGLDGKVICGSRVRVELSTGMPR 87

[Close]

BLAST of SMED30023281 vs. Ensembl Xenopus
Match: ENSXETT00000035364.1 (pep primary_assembly:Xenopus_tropicalis_v9.1:1:71113709:71115500:-1 gene:ENSXETG00000011190.1 transcript:ENSXETT00000035364.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 79.337 bits (194), Expect = 7.142e-19
Identity = 33/76 (43.42%), Postives = 51/76 (67.11%), Query Frame = -1

Query:  207 NKDEVKVYVGDLPKDANEKEIEKAFKDFGPIKSVWVARNPAGFAFVMFDDYRDAEDAVKELDGSVICGVRVRVEMS 434
            ++D   VY+G+L +  N   +E+AF  +GP++ +WVA+ P GFAFV F+   DA  AV+ L+G  +CG ++RVE S
Sbjct:    2 HRDNCNVYIGNLGQHVNRAVLEQAFGVYGPLRWIWVAQKPPGFAFVEFEKPHDAAAAVQGLNGKTLCGRQIRVEFS 77

[Close]

BLAST of SMED30023281 vs. Ensembl Mouse
Match: Srsf3 (serine/arginine-rich splicing factor 3 [Source:MGI Symbol;Acc:MGI:98285])

HSP 1 Score: 106.686 bits (265), Expect = 7.046e-30
Identity = 48/80 (60.00%), Postives = 63/80 (78.75%), Query Frame = -1

Query:  186 EVKVYVGDLPKDANEKEIEKAFKDFGPIKSVWVARNPAGFAFVMFDDYRDAEDAVKELDGSVICGVRVRVEMSHGRSRPK 425
            + KVYVG+L  + N+ E+E+AF  +GP++SVWVARNP GFAFV F+D RDA DAV+ELDG  +CG RVRVE+S+G  R +
Sbjct:    9 DCKVYVGNLGNNGNKTELERAFGYYGPLRSVWVARNPPGFAFVEFEDPRDAADAVRELDGRTLCGCRVRVELSNGEKRSR 88

[Close]

BLAST of SMED30023281 vs. Ensembl Mouse
Match: Srsf7 (serine/arginine-rich splicing factor 7 [Source:MGI Symbol;Acc:MGI:1926232])

HSP 1 Score: 106.301 bits (264), Expect = 1.252e-29
Identity = 49/78 (62.82%), Postives = 61/78 (78.21%), Query Frame = -1

Query:  192 EVKVYVGDLPKDANEKEIEKAFKDFGPIKSVWVARNPAGFAFVMFDDYRDAEDAVKELDGSVICGVRVRVEMSHGRSR 425
            E KVYVG+L   A + E+E+AF  +GP+++VW+ARNP GFAFV F+D RDAEDAV+ LDG VICG RVRVE+S G  R
Sbjct:   10 ETKVYVGNLGTGAGKGELERAFSYYGPLRTVWIARNPPGFAFVEFEDPRDAEDAVRGLDGKVICGSRVRVELSTGMPR 87

[Close]

BLAST of SMED30023281 vs. Ensembl Mouse
Match: Srsf3 (serine/arginine-rich splicing factor 3 [Source:MGI Symbol;Acc:MGI:98285])

HSP 1 Score: 106.686 bits (265), Expect = 2.415e-29
Identity = 48/78 (61.54%), Postives = 62/78 (79.49%), Query Frame = -1

Query:  192 EVKVYVGDLPKDANEKEIEKAFKDFGPIKSVWVARNPAGFAFVMFDDYRDAEDAVKELDGSVICGVRVRVEMSHGRSR 425
            + KVYVG+L  + N+ E+E+AF  +GP++SVWVARNP GFAFV F+D RDA DAV+ELDG  +CG RVRVE+S+G  R
Sbjct:    9 DCKVYVGNLGNNGNKTELERAFGYYGPLRSVWVARNPPGFAFVEFEDPRDAADAVRELDGRTLCGCRVRVELSNGEKR 86

[Close]

BLAST of SMED30023281 vs. Ensembl Mouse
Match: Srsf7 (serine/arginine-rich splicing factor 7 [Source:MGI Symbol;Acc:MGI:1926232])

HSP 1 Score: 106.301 bits (264), Expect = 9.326e-29
Identity = 49/78 (62.82%), Postives = 61/78 (78.21%), Query Frame = -1

Query:  192 EVKVYVGDLPKDANEKEIEKAFKDFGPIKSVWVARNPAGFAFVMFDDYRDAEDAVKELDGSVICGVRVRVEMSHGRSR 425
            E KVYVG+L   A + E+E+AF  +GP+++VW+ARNP GFAFV F+D RDAEDAV+ LDG VICG RVRVE+S G  R
Sbjct:   10 ETKVYVGNLGTGAGKGELERAFSYYGPLRTVWIARNPPGFAFVEFEDPRDAEDAVRGLDGKVICGSRVRVELSTGMPR 87

[Close]

BLAST of SMED30023281 vs. Ensembl Mouse
Match: Srsf7 (serine/arginine-rich splicing factor 7 [Source:MGI Symbol;Acc:MGI:1926232])

HSP 1 Score: 106.686 bits (265), Expect = 1.295e-28
Identity = 49/78 (62.82%), Postives = 61/78 (78.21%), Query Frame = -1

Query:  192 EVKVYVGDLPKDANEKEIEKAFKDFGPIKSVWVARNPAGFAFVMFDDYRDAEDAVKELDGSVICGVRVRVEMSHGRSR 425
            E KVYVG+L   A + E+E+AF  +GP+++VW+ARNP GFAFV F+D RDAEDAV+ LDG VICG RVRVE+S G  R
Sbjct:   10 ETKVYVGNLGTGAGKGELERAFSYYGPLRTVWIARNPPGFAFVEFEDPRDAEDAVRGLDGKVICGSRVRVELSTGMPR 87

[Close]

BLAST of SMED30023281 vs. UniProt/SwissProt
Match: sp|P84104|SRSF3_MOUSE (Serine/arginine-rich splicing factor 3 OS=Mus musculus OX=10090 GN=Srsf3 PE=1 SV=1)

HSP 1 Score: 106.686 bits (265), Expect = 1.689e-28
Identity = 48/78 (61.54%), Postives = 62/78 (79.49%), Query Frame = -1

Query:  192 EVKVYVGDLPKDANEKEIEKAFKDFGPIKSVWVARNPAGFAFVMFDDYRDAEDAVKELDGSVICGVRVRVEMSHGRSR 425
            + KVYVG+L  + N+ E+E+AF  +GP++SVWVARNP GFAFV F+D RDA DAV+ELDG  +CG RVRVE+S+G  R
Sbjct:    9 DCKVYVGNLGNNGNKTELERAFGYYGPLRSVWVARNPPGFAFVEFEDPRDAADAVRELDGRTLCGCRVRVELSNGEKR 86

[Close]

BLAST of SMED30023281 vs. UniProt/SwissProt
Match: sp|P84103|SRSF3_HUMAN (Serine/arginine-rich splicing factor 3 OS=Homo sapiens OX=9606 GN=SRSF3 PE=1 SV=1)

HSP 1 Score: 106.686 bits (265), Expect = 1.689e-28
Identity = 48/78 (61.54%), Postives = 62/78 (79.49%), Query Frame = -1

Query:  192 EVKVYVGDLPKDANEKEIEKAFKDFGPIKSVWVARNPAGFAFVMFDDYRDAEDAVKELDGSVICGVRVRVEMSHGRSR 425
            + KVYVG+L  + N+ E+E+AF  +GP++SVWVARNP GFAFV F+D RDA DAV+ELDG  +CG RVRVE+S+G  R
Sbjct:    9 DCKVYVGNLGNNGNKTELERAFGYYGPLRSVWVARNPPGFAFVEFEDPRDAADAVRELDGRTLCGCRVRVELSNGEKR 86

[Close]

BLAST of SMED30023281 vs. UniProt/SwissProt
Match: sp|Q3SZR8|SRSF3_BOVIN (Serine/arginine-rich splicing factor 3 OS=Bos taurus OX=9913 GN=SRSF3 PE=2 SV=1)

HSP 1 Score: 106.686 bits (265), Expect = 1.689e-28
Identity = 48/78 (61.54%), Postives = 62/78 (79.49%), Query Frame = -1

Query:  192 EVKVYVGDLPKDANEKEIEKAFKDFGPIKSVWVARNPAGFAFVMFDDYRDAEDAVKELDGSVICGVRVRVEMSHGRSR 425
            + KVYVG+L  + N+ E+E+AF  +GP++SVWVARNP GFAFV F+D RDA DAV+ELDG  +CG RVRVE+S+G  R
Sbjct:    9 DCKVYVGNLGNNGNKTELERAFGYYGPLRSVWVARNPPGFAFVEFEDPRDAADAVRELDGRTLCGCRVRVELSNGEKR 86

[Close]

BLAST of SMED30023281 vs. UniProt/SwissProt
Match: sp|Q16629|SRSF7_HUMAN (Serine/arginine-rich splicing factor 7 OS=Homo sapiens OX=9606 GN=SRSF7 PE=1 SV=1)

HSP 1 Score: 106.686 bits (265), Expect = 8.585e-28
Identity = 49/78 (62.82%), Postives = 61/78 (78.21%), Query Frame = -1

Query:  192 EVKVYVGDLPKDANEKEIEKAFKDFGPIKSVWVARNPAGFAFVMFDDYRDAEDAVKELDGSVICGVRVRVEMSHGRSR 425
            E KVYVG+L   A + E+E+AF  +GP+++VW+ARNP GFAFV F+D RDAEDAV+ LDG VICG RVRVE+S G  R
Sbjct:   10 ETKVYVGNLGTGAGKGELERAFSYYGPLRTVWIARNPPGFAFVEFEDPRDAEDAVRGLDGKVICGSRVRVELSTGMPR 87

[Close]

BLAST of SMED30023281 vs. UniProt/SwissProt
Match: sp|Q3T106|SRSF7_BOVIN (Serine/arginine-rich splicing factor 7 OS=Bos taurus OX=9913 GN=SRSF7 PE=2 SV=1)

HSP 1 Score: 106.301 bits (264), Expect = 1.083e-27
Identity = 49/78 (62.82%), Postives = 61/78 (78.21%), Query Frame = -1

Query:  192 EVKVYVGDLPKDANEKEIEKAFKDFGPIKSVWVARNPAGFAFVMFDDYRDAEDAVKELDGSVICGVRVRVEMSHGRSR 425
            E KVYVG+L   A + E+E+AF  +GP+++VW+ARNP GFAFV F+D RDAEDAV+ LDG VICG RVRVE+S G  R
Sbjct:   10 ETKVYVGNLGTGAGKGELERAFSYYGPLRTVWIARNPPGFAFVEFEDPRDAEDAVRGLDGKVICGSRVRVELSTGMPR 87

[Close]

BLAST of SMED30023281 vs. TrEMBL
Match: A0A3S1C6J9 (Uncharacterized protein (Fragment) OS=Elysia chlorotica OX=188477 GN=EGW08_007889 PE=4 SV=1)

HSP 1 Score: 141.354 bits (355), Expect = 7.858e-39
Identity = 65/95 (68.42%), Postives = 78/95 (82.11%), Query Frame = -1

Query:  162 YMSKNKDEVKVYVGDLPKDANEKEIEKAFKDFGPIKSVWVARNPAGFAFVMFDDYRDAEDAVKELDGSVICGVRVRVEMSHGRSRPKPWANQSRN 446
            Y  +  ++ KVYVGDLPKDA+EKE+EKAF  +G ++SVWVARNP GFAFV F+DYRDAED+VKELDG+ ICGVRVRVE+S G+ RPKPW   SR 
Sbjct:   40 YGGRVPNDCKVYVGDLPKDASEKEVEKAFSYYGRLRSVWVARNPPGFAFVEFEDYRDAEDSVKELDGTTICGVRVRVELSSGKVRPKPWLRGSRG 134

[Close]

BLAST of SMED30023281 vs. TrEMBL
Match: T1FXH9 (Uncharacterized protein OS=Helobdella robusta OX=6412 GN=20213527 PE=4 SV=1)

HSP 1 Score: 133.265 bits (334), Expect = 7.354e-37
Identity = 62/82 (75.61%), Postives = 69/82 (84.15%), Query Frame = -1

Query:  180 EVKVYVGDLPKDANEKEIEKAFKDFGPIKSVWVARNPAGFAFVMFDDYRDAEDAVKELDGSVICGVRVRVEMSHGRSRPKPW 425
            + K+YVGDLPKDA E E+E AF  FGPIKS+WVARNPAGFAFV F+D RDAEDAVK LDGS ICG RVRVE S G+S+PKPW
Sbjct:    9 DCKIYVGDLPKDATEDELESAFSTFGPIKSLWVARNPAGFAFVEFEDVRDAEDAVKTLDGSNICGGRVRVEFSTGKSKPKPW 90

[Close]

BLAST of SMED30023281 vs. TrEMBL
Match: A0A564Z500 (Uncharacterized protein OS=Hymenolepis diminuta OX=6216 GN=WMSIL1_LOCUS12459 PE=4 SV=1)

HSP 1 Score: 133.265 bits (334), Expect = 9.194e-37
Identity = 59/102 (57.84%), Postives = 78/102 (76.47%), Query Frame = -1

Query:  138 MSKNKDEVKVYVGDLPKDANEKEIEKAFKDFGPIKSVWVARNPAGFAFVMFDDYRDAEDAVKELDGSVICGVRVRVEMSHGRSRPKPWANQSRNSRYENSND 443
            MS+++   KVYVGDLP+DA+EKE+ + F  +G +++VWVARNP GFAF+ FDD  DAEDAV++LDG+VICGVR RVE+S+GR+RPKPW    R    +   D
Sbjct:    1 MSRSRSSTKVYVGDLPRDASEKELARVFDRYGTVRNVWVARNPPGFAFIEFDDPADAEDAVRDLDGTVICGVRARVELSNGRTRPKPWLRGDRGRESDRRGD 102

[Close]

BLAST of SMED30023281 vs. TrEMBL
Match: A0A448X1Q8 (Uncharacterized protein OS=Protopolystoma xenopodis OX=117903 GN=PXEA_LOCUS19407 PE=4 SV=1)

HSP 1 Score: 131.339 bits (329), Expect = 3.541e-36
Identity = 60/105 (57.14%), Postives = 80/105 (76.19%), Query Frame = -1

Query:  132 MSKNKD-EVKVYVGDLPKDANEKEIEKAFKDFGPIKSVWVARNPAGFAFVMFDDYRDAEDAVKELDGSVICGVRVRVEMSHGRSRPKPWANQSRNSRYENSNDYN 443
            MS+  D   K+YVGDLP++A+E+EIE+AF+ +G +++VWVARNP GFAFV FDD  DA DAV++LDG++ICGVR RVE+S G+SRPKPW    R S    S  ++
Sbjct:    1 MSRRVDYSAKIYVGDLPREASEREIEQAFRSYGKLRNVWVARNPPGFAFVEFDDATDARDAVRDLDGTIICGVRARVELSTGKSRPKPWLRGGRGSSPRRSRPFD 105

[Close]

BLAST of SMED30023281 vs. TrEMBL
Match: A0A0R3TNV0 (Uncharacterized protein OS=Rodentolepis nana OX=102285 GN=HNAJ_LOCUS9087 PE=4 SV=1)

HSP 1 Score: 131.339 bits (329), Expect = 4.818e-36
Identity = 57/88 (64.77%), Postives = 75/88 (85.23%), Query Frame = -1

Query:  180 MSKNKDEVKVYVGDLPKDANEKEIEKAFKDFGPIKSVWVARNPAGFAFVMFDDYRDAEDAVKELDGSVICGVRVRVEMSHGRSRPKPW 443
            MS+++   KVYVGDLP+DA+EKE+ + F  +G +++VWVARNP GFAF+ FDD  DAEDAV++LDG+VICGVR RVE+S+GR+RPKPW
Sbjct:    1 MSRSRSSTKVYVGDLPRDASEKELARVFDRYGTVRNVWVARNPPGFAFIEFDDPADAEDAVRDLDGTVICGVRARVELSNGRTRPKPW 88

[Close]

BLAST of SMED30023281 vs. Ensembl Cavefish
Match: srsf7a (serine and arginine rich splicing factor 7a [Source:ZFIN;Acc:ZDB-GENE-040426-1798])

HSP 1 Score: 110.538 bits (275), Expect = 3.603e-30
Identity = 54/94 (57.45%), Postives = 71/94 (75.53%), Query Frame = -1

Query:  165 YMSKNKDEVKVYVGDLPKDANEKEIEKAFKDFGPIKSVWVARNPAGFAFVMFDDYRDAEDAVKELDGSVICGVRVRVEMSHGRSRPKPWANQSR 446
            Y S+++ + KVYVGDL   A + E+E+AF  +GP++SVWVARNP GFAFV F+D RDAEDAVK +DG V+CG R+RVEMS+G +R   +   SR
Sbjct:    3 YSSRSQADCKVYVGDLGNGAAKGELERAFSYYGPLRSVWVARNPPGFAFVEFEDSRDAEDAVKGMDG-VLCGNRIRVEMSNGMTRKSRFGRPSR 95

[Close]

BLAST of SMED30023281 vs. Ensembl Cavefish
Match: srsf7b (serine and arginine rich splicing factor 7 [Source:NCBI gene;Acc:103037410])

HSP 1 Score: 108.997 bits (271), Expect = 7.602e-30
Identity = 49/83 (59.04%), Postives = 64/83 (77.11%), Query Frame = -1

Query:  192 SKNKDEVKVYVGDLPKDANEKEIEKAFKDFGPIKSVWVARNPAGFAFVMFDDYRDAEDAVKELDGSVICGVRVRVEMSHGRSR 440
            S++  E KVYVG+L   A + E+E+AF  +GP+++VW+ARNP GFAFV F+D RDAEDAV+ LDG VICG +VRVE+S G  R
Sbjct:    5 SRHAGETKVYVGNLGTGAGKGELERAFGYYGPLRTVWIARNPPGFAFVEFEDPRDAEDAVRGLDGKVICGTQVRVELSTGMPR 87

[Close]

BLAST of SMED30023281 vs. Ensembl Cavefish
Match: srsf7b (serine and arginine rich splicing factor 7 [Source:NCBI gene;Acc:103037410])

HSP 1 Score: 109.383 bits (272), Expect = 9.297e-30
Identity = 49/83 (59.04%), Postives = 64/83 (77.11%), Query Frame = -1

Query:  192 SKNKDEVKVYVGDLPKDANEKEIEKAFKDFGPIKSVWVARNPAGFAFVMFDDYRDAEDAVKELDGSVICGVRVRVEMSHGRSR 440
            S++  E KVYVG+L   A + E+E+AF  +GP+++VW+ARNP GFAFV F+D RDAEDAV+ LDG VICG +VRVE+S G  R
Sbjct:    5 SRHAGETKVYVGNLGTGAGKGELERAFGYYGPLRTVWIARNPPGFAFVEFEDPRDAEDAVRGLDGKVICGTQVRVELSTGMPR 87

[Close]

BLAST of SMED30023281 vs. Ensembl Cavefish
Match: srsf7b (serine and arginine rich splicing factor 7 [Source:NCBI gene;Acc:103037410])

HSP 1 Score: 108.997 bits (271), Expect = 1.059e-29
Identity = 49/83 (59.04%), Postives = 64/83 (77.11%), Query Frame = -1

Query:  192 SKNKDEVKVYVGDLPKDANEKEIEKAFKDFGPIKSVWVARNPAGFAFVMFDDYRDAEDAVKELDGSVICGVRVRVEMSHGRSR 440
            S++  E KVYVG+L   A + E+E+AF  +GP+++VW+ARNP GFAFV F+D RDAEDAV+ LDG VICG +VRVE+S G  R
Sbjct:    5 SRHAGETKVYVGNLGTGAGKGELERAFGYYGPLRTVWIARNPPGFAFVEFEDPRDAEDAVRGLDGKVICGTQVRVELSTGMPR 87

[Close]

BLAST of SMED30023281 vs. Ensembl Cavefish
Match: srsf3a (serine/arginine-rich splicing factor 3-like [Source:NCBI gene;Acc:103045376])

HSP 1 Score: 107.071 bits (266), Expect = 1.063e-29
Identity = 48/78 (61.54%), Postives = 61/78 (78.21%), Query Frame = -1

Query:  192 EVKVYVGDLPKDANEKEIEKAFKDFGPIKSVWVARNPAGFAFVMFDDYRDAEDAVKELDGSVICGVRVRVEMSHGRSR 425
            + KVYVG+L    ++ E+E+AF  +GP++SVWVARNP GFAFV F+D+RDA DAV+ELDG  +CG RVRVEMS G  R
Sbjct:   13 DCKVYVGNLGNSGDKTELERAFGYYGPLRSVWVARNPPGFAFVEFEDHRDAADAVRELDGRTLCGCRVRVEMSSGEKR 90

[Close]

BLAST of SMED30023281 vs. Ensembl Sea Lamprey
Match: srsf3a (serine and arginine rich splicing factor 3a [Source:ZFIN;Acc:ZDB-GENE-030616-631])

HSP 1 Score: 107.071 bits (266), Expect = 3.104e-30
Identity = 55/96 (57.29%), Postives = 69/96 (71.88%), Query Frame = -1

Query:  147 EVKVYVGDLPKDANEKEIEKAFKDFGPIKSVWVARNPAGFAFVMFDDYRDAEDAVKELDGSVICGVRVRVEMSHGRSR---PKPWANQSRNSRYEN 425
            + KVYVG+L  + N+ E+E++F  +GP++SVWVAR P GFAFV F+D RDA DAV+ELDG  +CG RVRVE S GRSR   P P  N  R  RYE+
Sbjct:   14 DCKVYVGNLGNNGNKAELERSFSYYGPLRSVWVARAPPGFAFVEFEDPRDAGDAVRELDGRTLCGSRVRVEHSSGRSRNRGPPPAWN--RRPRYED 107

[Close]

BLAST of SMED30023281 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000006373.1 (pep scaffold:Pmarinus_7.0:GL478975:164155:166212:-1 gene:ENSPMAG00000005755.1 transcript:ENSPMAT00000006373.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 107.457 bits (267), Expect = 1.477e-29
Identity = 53/93 (56.99%), Postives = 66/93 (70.97%), Query Frame = -1

Query:  150 EVKVYVGDLPKDANEKEIEKAFKDFGPIKSVWVARNPAGFAFVMFDDYRDAEDAVKELDGSVICGVRVRVEMSHGRSRPK-PWANQSRNSRYE 425
            + KVYVG+L   A E ++EK F  +GP++SVWVARNP GFAFV F+D RDA D+ +ELDG ++CG RVRVEMS G  R K  W+ Q   SR E
Sbjct:   14 DCKVYVGNLGSGATEYDLEKVFSYYGPLRSVWVARNPPGFAFVQFEDARDARDSCRELDGRLVCGSRVRVEMSTGERRQKRGWSAQPPWSRCE 106

[Close]

BLAST of SMED30023281 vs. Ensembl Sea Lamprey
Match: srsf7a (serine and arginine rich splicing factor 7a [Source:ZFIN;Acc:ZDB-GENE-040426-1798])

HSP 1 Score: 82.4185 bits (202), Expect = 2.524e-20
Identity = 39/73 (53.42%), Postives = 53/73 (72.60%), Query Frame = -1

Query:  192 VGDLPKDANEKEIEKAFKDFGPIKSVWVARNPAGFAFVMFDDYRDAEDAVKELDGSVICGVRVRVEMSHGRSR 410
            VG+L   A + E+ +AF  +G +++VWVARNP   AFV ++D RDAEDAV+ LDG ++CG RV VEMS+G  R
Sbjct:    1 VGNLGTRAAKGELWQAFGYYGALRNVWVARNPPAVAFVEYEDTRDAEDAVRGLDGRLLCGSRVSVEMSNGVGR 73

[Close]

BLAST of SMED30023281 vs. Ensembl Sea Lamprey
Match: srsf7a (serine and arginine rich splicing factor 7a [Source:ZFIN;Acc:ZDB-GENE-040426-1798])

HSP 1 Score: 82.0333 bits (201), Expect = 2.804e-20
Identity = 39/73 (53.42%), Postives = 53/73 (72.60%), Query Frame = -1

Query:  192 VGDLPKDANEKEIEKAFKDFGPIKSVWVARNPAGFAFVMFDDYRDAEDAVKELDGSVICGVRVRVEMSHGRSR 410
            VG+L   A + E+ +AF  +G +++VWVARNP   AFV ++D RDAEDAV+ LDG ++CG RV VEMS+G  R
Sbjct:    1 VGNLGTRAAKGELWQAFGYYGALRNVWVARNPPAVAFVEYEDTRDAEDAVRGLDGRLLCGSRVSVEMSNGVGR 73

[Close]

BLAST of SMED30023281 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000010377.1 (pep scaffold:Pmarinus_7.0:GL481287:28:8451:-1 gene:ENSPMAG00000009397.1 transcript:ENSPMAT00000010377.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 64.6994 bits (156), Expect = 2.361e-13
Identity = 34/76 (44.74%), Postives = 47/76 (61.84%), Query Frame = -1

Query:  192 KVYVGDLPKDANEKEIEKAFKDFGPIKSVWVARNPAGFAFVMFDDYRDAEDAVKELDGSVICGVRVRVEMSHGRSR 419
            ++Y+G+L   A +K+IE+ FK +G I  + +     GF FV FDD RDA DAV EL G  +CG RV +E + G  R
Sbjct:    3 RIYLGNLSYRARDKDIERFFKGYGKIVEISIKH---GFGFVEFDDTRDANDAVCELSGRELCGERVVIEHARGPKR 75

[Close]

BLAST of SMED30023281 vs. Ensembl Yeast
Match: MRD1 (Essential conserved small ribosomal subunit (40s) synthesis factor; component of the 90S preribosome; required for production of 18S rRNA and small ribosomal subunit; contains five consensus RNA-binding domains and binds to the pre-rRNA at two sites within the 18S region [Source:SGD;Acc:S000006316])

HSP 1 Score: 46.595 bits (109), Expect = 4.380e-7
Identity = 28/75 (37.33%), Postives = 44/75 (58.67%), Query Frame = -1

Query:  222 NKDEVKVYVGDLPKDANEKEIEKAFKDFGPIKSVWVA----RNPAGFAFVMFDDYRDAEDAVKELDGSVICGVRV 434
            NK   K+ V +LP +A  K++ + F  FG +KSV V     ++  GFAFV F   ++AE+A+ +L G  + G R+
Sbjct:  759 NKKSGKIIVKNLPFEATRKDVFELFNSFGQLKSVRVPKKFDKSARGFAFVEFLLPKEAENAMDQLHGVHLLGRRL 833

[Close]

BLAST of SMED30023281 vs. Ensembl Yeast
Match: NPL3 (RNA-binding protein; promotes elongation, regulates termination, and carries poly(A) mRNA from nucleus to cytoplasm; represses translation initiation by binding eIF4G; required for pre-mRNA splicing; interacts with E3 ubiquitin ligase Bre1p, linking histone ubiquitination to mRNA processing; may have role in telomere maintenance; dissociation from mRNAs promoted by Mtr10p; phosphorylated by Sky1p in cytoplasm; protein abundance increases in response to DNA replication stress [Source:SGD;Acc:S000002840])

HSP 1 Score: 43.5134 bits (101), Expect = 4.889e-6
Identity = 22/69 (31.88%), Postives = 35/69 (50.72%), Query Frame = -1

Query:  216 VKVYVGDLPKDANEKEIEKAFKDFGPIKSVWVARNPAGFAFVMFDDYRDAEDAVKELDGSVICGVRVRV 422
             +++V   P D  E E+ + F  FGP+K V +     GFAFV F++   A  A++E+ G       + V
Sbjct:  125 TRLFVRPFPLDVQESELNEIFGPFGPMKEVKILN---GFAFVEFEEAESAAKAIEEVHGKSFANQPLEV 190

[Close]

BLAST of SMED30023281 vs. Ensembl Nematostella
Match: EDO31304 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SYA7])

HSP 1 Score: 107.071 bits (266), Expect = 4.983e-31
Identity = 50/80 (62.50%), Postives = 62/80 (77.50%), Query Frame = -1

Query:  183 VKVYVGDLPKDANEKEIEKAFKDFGPIKSVWVARNPAGFAFVMFDDYRDAEDAVKELDGSVICGVRVRVEMSHGRSRPKP 422
             KVY+G L  +A+++EIE  F  +GP+K VWVARNP GFAF +FDD RDAEDAV+ELDG  ICG RVRVE++ G SR +P
Sbjct:    2 TKVYIGSLGDNASKREIENEFGYYGPLKDVWVARNPPGFAFCIFDDRRDAEDAVRELDGRYICGQRVRVELAKGPSRGRP 81

[Close]

BLAST of SMED30023281 vs. Ensembl Nematostella
Match: EDO31305 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SYA8])

HSP 1 Score: 106.301 bits (264), Expect = 3.474e-30
Identity = 46/79 (58.23%), Postives = 64/79 (81.01%), Query Frame = -1

Query:  183 KVYVGDLPKDANEKEIEKAFKDFGPIKSVWVARNPAGFAFVMFDDYRDAEDAVKELDGSVICGVRVRVEMSHGRSRPKP 419
            +VY+G++  +A+++EIE+ F+ FGP++ VWVARNP GFAF +F+D RDAEDAV+ELDG  ICG R RVE++ G SR +P
Sbjct:    3 RVYIGNIGDNASKREIEREFETFGPLRDVWVARNPPGFAFCVFEDRRDAEDAVRELDGRYICGQRARVELAKGPSRGRP 81

[Close]

BLAST of SMED30023281 vs. Ensembl Nematostella
Match: EDO30175 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T1I7])

HSP 1 Score: 93.5893 bits (231), Expect = 1.414e-25
Identity = 41/73 (56.16%), Postives = 58/73 (79.45%), Query Frame = -1

Query:  204 VKVYVGDLPKDANEKEIEKAFKDFGPIKSVWVARNPAGFAFVMFDDYRDAEDAVKELDGSVICGVRVRVEMSH 422
             K+YVG+L ++A+  E+E+AF+ FG +  VWVARNP GFAFV ++DYRDAE+AV+ELDG+ +C   +RVE S+
Sbjct:    3 TKLYVGNLGRNADSSELERAFEKFGRLSKVWVARNPPGFAFVEYEDYRDAEEAVRELDGANVCDRTIRVEFSN 75

[Close]

BLAST of SMED30023281 vs. Ensembl Nematostella
Match: EDO30178 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T1I6])

HSP 1 Score: 90.8929 bits (224), Expect = 2.336e-24
Identity = 43/73 (58.90%), Postives = 54/73 (73.97%), Query Frame = -1

Query:  204 VKVYVGDLPKDANEKEIEKAFKDFGPIKSVWVARNPAGFAFVMFDDYRDAEDAVKELDGSVICGVRVRVEMSH 422
             KV+VG+L K+ +  E+ +AFK +G ++ VWVARNP GFAFV F D RDA DAV  LDG  ICG RV+VE+SH
Sbjct:    4 TKVWVGNLGKEGDRHELWEAFKSYGELRDVWVARNPPGFAFVEFYDARDARDAVDALDGERICGQRVKVELSH 76

[Close]

BLAST of SMED30023281 vs. Ensembl Nematostella
Match: EDO44646 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RVI6])

HSP 1 Score: 70.8626 bits (172), Expect = 1.361e-15
Identity = 42/87 (48.28%), Postives = 53/87 (60.92%), Query Frame = -1

Query:  183 NKDEVKVYVGDLPKDANEKEIEKAFKDFGPIKSVWVA-RNPAG--FAFVMFDDYRDAEDAVKELDGSVICGVRVRVEMSHGRSRPKP 434
            N ++ +VYVG+LP+D  EK++   F  +G I  V +  R  AG  FAFV F+D RDAEDAVK  DG    G R+RVE   G S P P
Sbjct:    5 NSNDCRVYVGNLPQDVREKDLHDIFYKYGHIADVDLKNRRGAGPPFAFVEFEDPRDAEDAVKGRDGHEFDGYRIRVEFPRGGSGPPP 91

[Close]

BLAST of SMED30023281 vs. Ensembl Medaka
Match: SRSF3 (serine/arginine-rich splicing factor 3 [Source:NCBI gene;Acc:101168533])

HSP 1 Score: 108.612 bits (270), Expect = 1.049e-29
Identity = 51/93 (54.84%), Postives = 67/93 (72.04%), Query Frame = -1

Query:  165 EVKVYVGDLPKDANEKEIEKAFKDFGPIKSVWVARNPAGFAFVMFDDYRDAEDAVKELDGSVICGVRVRVEMSHGRSR------PKPWANQSR 425
            + KVYVG+L  + N+ E+E+AF  +GP++SVWVARNP GFAFV F+D RDA DAV+ELDG  +CG RVRVE+S+G  R      P  W+ + R
Sbjct:   74 DCKVYVGNLGNNGNKTELERAFGYYGPLRSVWVARNPPGFAFVEFEDPRDATDAVRELDGRTLCGCRVRVELSNGEKRSRSRGAPPSWSRRPR 166

[Close]

BLAST of SMED30023281 vs. Ensembl Medaka
Match: SRSF3 (serine/arginine-rich splicing factor 3 [Source:NCBI gene;Acc:101168533])

HSP 1 Score: 108.612 bits (270), Expect = 1.576e-29
Identity = 51/93 (54.84%), Postives = 67/93 (72.04%), Query Frame = -1

Query:  165 EVKVYVGDLPKDANEKEIEKAFKDFGPIKSVWVARNPAGFAFVMFDDYRDAEDAVKELDGSVICGVRVRVEMSHGRSR------PKPWANQSR 425
            + KVYVG+L  + N+ E+E+AF  +GP++SVWVARNP GFAFV F+D RDA DAV+ELDG  +CG RVRVE+S+G  R      P  W+ + R
Sbjct:   74 DCKVYVGNLGNNGNKTELERAFGYYGPLRSVWVARNPPGFAFVEFEDPRDATDAVRELDGRTLCGCRVRVELSNGEKRSRSRGAPPSWSRRPR 166

[Close]

BLAST of SMED30023281 vs. Ensembl Medaka
Match: srsf7a (serine/arginine-rich splicing factor 7 [Source:NCBI gene;Acc:101155211])

HSP 1 Score: 108.612 bits (270), Expect = 2.216e-29
Identity = 51/78 (65.38%), Postives = 62/78 (79.49%), Query Frame = -1

Query:  192 EVKVYVGDLPKDANEKEIEKAFKDFGPIKSVWVARNPAGFAFVMFDDYRDAEDAVKELDGSVICGVRVRVEMSHGRSR 425
            + KVYVGDL   A + E+E+AF  +GP++SVWVARNP GFAFV F+D RDAEDAVK +DG ++CG RVRVEMS G SR
Sbjct:   15 DCKVYVGDLGNGAAKGELERAFSYYGPLRSVWVARNPPGFAFVEFEDPRDAEDAVKGMDGKLLCGSRVRVEMSTGLSR 92

[Close]

BLAST of SMED30023281 vs. Ensembl Medaka
Match: srsf7a (serine/arginine-rich splicing factor 7 [Source:NCBI gene;Acc:101155211])

HSP 1 Score: 108.227 bits (269), Expect = 4.289e-29
Identity = 51/78 (65.38%), Postives = 62/78 (79.49%), Query Frame = -1

Query:  192 EVKVYVGDLPKDANEKEIEKAFKDFGPIKSVWVARNPAGFAFVMFDDYRDAEDAVKELDGSVICGVRVRVEMSHGRSR 425
            + KVYVGDL   A + E+E+AF  +GP++SVWVARNP GFAFV F+D RDAEDAVK +DG ++CG RVRVEMS G SR
Sbjct:   15 DCKVYVGDLGNGAAKGELERAFSYYGPLRSVWVARNPPGFAFVEFEDPRDAEDAVKGMDGKLLCGSRVRVEMSTGLSR 92

[Close]

BLAST of SMED30023281 vs. Ensembl Medaka
Match: srsf3a (serine and arginine rich splicing factor 3a [Source:ZFIN;Acc:ZDB-GENE-030616-631])

HSP 1 Score: 105.145 bits (261), Expect = 7.628e-29
Identity = 47/80 (58.75%), Postives = 62/80 (77.50%), Query Frame = -1

Query:  186 EVKVYVGDLPKDANEKEIEKAFKDFGPIKSVWVARNPAGFAFVMFDDYRDAEDAVKELDGSVICGVRVRVEMSHGRSRPK 425
            + KVYVG+L  + N+ E+E+AF  +GP+++VWVARNP GFAFV F+D RDA DAV+ELDG  +CG RVRVE+S G  R +
Sbjct:   11 DCKVYVGNLGNNGNKTELERAFGYYGPLRTVWVARNPPGFAFVEFEDPRDASDAVRELDGRTMCGCRVRVELSTGEKRSR 90

[Close]

BLAST of SMED30023281 vs. Planmine SMEST
Match: SMESG000006493.1 (SMESG000006493.1)

HSP 1 Score: 221.476 bits (563), Expect = 2.631e-75
Identity = 107/107 (100.00%), Postives = 107/107 (100.00%), Query Frame = -1

Query:  123 MSKNKDEVKVYVGDLPKDANEKEIEKAFKDFGPIKSVWVARNPAGFAFVMFDDYRDAEDAVKELDGSVICGVRVRVEMSHGRSRPKPWANQSRNSRYENSNDYNDKK 443
            MSKNKDEVKVYVGDLPKDANEKEIEKAFKDFGPIKSVWVARNPAGFAFVMFDDYRDAEDAVKELDGSVICGVRVRVEMSHGRSRPKPWANQSRNSRYENSNDYNDKK
Sbjct:    1 MSKNKDEVKVYVGDLPKDANEKEIEKAFKDFGPIKSVWVARNPAGFAFVMFDDYRDAEDAVKELDGSVICGVRVRVEMSHGRSRPKPWANQSRNSRYENSNDYNDKK 107

[Close]

BLAST of SMED30023281 vs. Planmine SMEST
Match: SMESG000045501.1 (SMESG000045501.1)

HSP 1 Score: 71.633 bits (174), Expect = 5.216e-15
Identity = 35/71 (49.30%), Postives = 51/71 (71.83%), Query Frame = -1

Query:  207 KVYVGDLPKDANEKEIEKAFKDFGPIKSVWVARNPAGFAFVMFDDYRDAEDAVKELDGSVICGVRVRVEMS 419
            ++YVG+LP+   E++I+K F+++G I+SV +     G+ FV  DD RDAEDAV +LDGS + G RV VEM+
Sbjct:    3 RLYVGNLPRRIEERDIKKFFREYGRIRSVDIKN---GYGFVEIDDRRDAEDAVHDLDGSKLLGERVTVEMA 70

[Close]

BLAST of SMED30023281 vs. Planmine SMEST
Match: SMESG000013050.1 (SMESG000013050.1)

HSP 1 Score: 67.3958 bits (163), Expect = 4.105e-14
Identity = 35/76 (46.05%), Postives = 48/76 (63.16%), Query Frame = -1

Query:  204 DEVKVYVGDLPKDANEKEIEKAFKDFGPIKSVWVA-RNPAGFAFVMFDDYRDAEDAVKELDGSVICGVRVRVEMSH 428
            +E K+YVG+LP D+  ++IEK F  FG I  + +  RN   FAF+ FDD RDAEDA++E D   + G  +RVE   
Sbjct:    3 NERKIYVGNLPPDSTSRDIEKLFSCFGKIIDIDIKNRNGPPFAFIQFDDRRDAEDAIRERDNYDLDGFSLRVEFPR 78

[Close]

BLAST of SMED30023281 vs. Planmine SMEST
Match: SMESG000013050.1 (SMESG000013050.1)

HSP 1 Score: 68.1662 bits (165), Expect = 4.726e-14
Identity = 35/74 (47.30%), Postives = 48/74 (64.86%), Query Frame = -1

Query:  210 DEVKVYVGDLPKDANEKEIEKAFKDFGPIKSVWVA-RNPAGFAFVMFDDYRDAEDAVKELDGSVICGVRVRVEM 428
            +E K+YVG+LP D+  ++IEK F  FG I  + +  RN   FAF+ FDD RDAEDA++E D   + G  +RVE 
Sbjct:    3 NERKIYVGNLPPDSTSRDIEKLFSCFGKIIDIDIKNRNGPPFAFIQFDDRRDAEDAIRERDNYDLDGFSLRVEF 76

[Close]

BLAST of SMED30023281 vs. Planmine SMEST
Match: SMESG000039881.1 (SMESG000039881.1)

HSP 1 Score: 63.5438 bits (153), Expect = 3.274e-12
Identity = 29/78 (37.18%), Postives = 48/78 (61.54%), Query Frame = -1

Query:  186 KVYVGDLPKDANEKEIEKAFKDFGPIKSVWVARNPAGFAFVMFDDYRDAEDAVKELDGSVICGVRVRVEMSHGRSRPK 419
            ++++G L     + +IE+ F+ FG +  VWVAR+P GF+F++F    DAE AV +++     G R++VE +   S PK
Sbjct:   10 RIFIGGLNPKVTKYDIEREFERFGKVTDVWVARSPPGFSFLIFKHAGDAEYAVAKMNDRKFMGERIKVEHARNPSNPK 87

The following BLAST results are available for this feature:

BLAST of SMED30023281 vs. Ensembl Human
BLAST of SMED30023281 vs. Ensembl Celegans
BLAST of SMED30023281 vs. Ensembl Fly
BLAST of SMED30023281 vs. Ensembl Zebrafish
BLAST of SMED30023281 vs. Ensembl Xenopus
BLAST of SMED30023281 vs. Ensembl Mouse
BLAST of SMED30023281 vs. UniProt/SwissProt
BLAST of SMED30023281 vs. TrEMBL
BLAST of SMED30023281 vs. Ensembl Cavefish
BLAST of SMED30023281 vs. Ensembl Sea Lamprey
BLAST of SMED30023281 vs. Ensembl Yeast
BLAST of SMED30023281 vs. Ensembl Nematostella
BLAST of SMED30023281 vs. Ensembl Medaka
BLAST of SMED30023281 vs. Planmine SMEST

BLAST of SMED30023281 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5


Match Name	E-value	Identity	Description
SRSF3	1.026e-29	60.00	serine and arginine rich splicing factor 3 [Source...	[more]
SRSF7	1.823e-29	62.82	serine and arginine rich splicing factor 7 [Source...	[more]
SRSF7	1.823e-29	62.82	serine and arginine rich splicing factor 7 [Source...	[more]
SRSF7	2.077e-29	62.82	serine and arginine rich splicing factor 7 [Source...	[more]
SRSF7	2.077e-29	62.82	serine and arginine rich splicing factor 7 [Source...	[more]

BLAST of SMED30023281 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5


Match Name	E-value	Identity	Description
rsp-6	1.213e-27	65.75	Probable splicing factor, arginine/serine-rich 6 ...	[more]
Y57G11A.5	8.215e-21	52.63	pep chromosome:WBcel235:IV:14511884:14512505:-1 ge...	[more]
rsp-3	2.704e-15	54.79	Probable splicing factor, arginine/serine-rich 3 ...	[more]
rsp-3	2.704e-15	54.79	Probable splicing factor, arginine/serine-rich 3 ...	[more]
rsp-3	2.704e-15	54.79	Probable splicing factor, arginine/serine-rich 3 ...	[more]

BLAST of SMED30023281 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5


Match Name	E-value	Identity	Description
Rbp1	5.131e-27	62.86	gene:FBgn0260944 transcript:FBtr0082277	[more]
Rbp1	2.265e-26	62.86	gene:FBgn0260944 transcript:FBtr0082275	[more]
Rbp1-like	9.514e-26	62.86	gene:FBgn0030479 transcript:FBtr0339838	[more]
Rbp1-like	9.514e-26	62.86	gene:FBgn0030479 transcript:FBtr0073790	[more]
Rbp1-like	5.918e-25	61.97	gene:FBgn0030479 transcript:FBtr0304001	[more]

BLAST of SMED30023281 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
srsf7a	7.644e-31	59.77	serine and arginine rich splicing factor 7a [Sourc...	[more]
srsf7a	7.913e-31	59.77	serine and arginine rich splicing factor 7a [Sourc...	[more]
srsf7a	1.082e-30	64.10	serine and arginine rich splicing factor 7a [Sourc...	[more]
srsf7b	2.207e-30	59.52	serine and arginine rich splicing factor 7b [Sourc...	[more]
srsf7b	2.249e-30	59.52	serine and arginine rich splicing factor 7b [Sourc...	[more]

BLAST of SMED30023281 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5


Match Name	E-value	Identity	Description
phex	1.044e-29	60.00	phosphate regulating endopeptidase homolog X-linke...	[more]
phex	2.801e-29	61.54	phosphate regulating endopeptidase homolog X-linke...	[more]
sms	5.317e-29	62.82	spermine synthase [Source:Xenbase;Acc:XB-GENE-9535...	[more]
sms	7.312e-29	62.82	spermine synthase [Source:Xenbase;Acc:XB-GENE-9535...	[more]
ENSXETT00000035364.1	7.142e-19	43.42	pep primary_assembly:Xenopus_tropicalis_v9.1:1:711...	[more]

BLAST of SMED30023281 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5


Match Name	E-value	Identity	Description
Srsf3	7.046e-30	60.00	serine/arginine-rich splicing factor 3 [Source:MGI...	[more]
Srsf7	1.252e-29	62.82	serine/arginine-rich splicing factor 7 [Source:MGI...	[more]
Srsf3	2.415e-29	61.54	serine/arginine-rich splicing factor 3 [Source:MGI...	[more]
Srsf7	9.326e-29	62.82	serine/arginine-rich splicing factor 7 [Source:MGI...	[more]
Srsf7	1.295e-28	62.82	serine/arginine-rich splicing factor 7 [Source:MGI...	[more]

BLAST of SMED30023281 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5


Match Name	E-value	Identity	Description
sp\|P84104\|SRSF3_MOUSE	1.689e-28	61.54	Serine/arginine-rich splicing factor 3 OS=Mus musc...	[more]
sp\|P84103\|SRSF3_HUMAN	1.689e-28	61.54	Serine/arginine-rich splicing factor 3 OS=Homo sap...	[more]
sp\|Q3SZR8\|SRSF3_BOVIN	1.689e-28	61.54	Serine/arginine-rich splicing factor 3 OS=Bos taur...	[more]
sp\|Q16629\|SRSF7_HUMAN	8.585e-28	62.82	Serine/arginine-rich splicing factor 7 OS=Homo sap...	[more]
sp\|Q3T106\|SRSF7_BOVIN	1.083e-27	62.82	Serine/arginine-rich splicing factor 7 OS=Bos taur...	[more]

BLAST of SMED30023281 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A3S1C6J9	7.858e-39	68.42	Uncharacterized protein (Fragment) OS=Elysia chlor...	[more]
T1FXH9	7.354e-37	75.61	Uncharacterized protein OS=Helobdella robusta OX=6...	[more]
A0A564Z500	9.194e-37	57.84	Uncharacterized protein OS=Hymenolepis diminuta OX...	[more]
A0A448X1Q8	3.541e-36	57.14	Uncharacterized protein OS=Protopolystoma xenopodi...	[more]
A0A0R3TNV0	4.818e-36	64.77	Uncharacterized protein OS=Rodentolepis nana OX=10...	[more]

BLAST of SMED30023281 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
srsf7a	3.603e-30	57.45	serine and arginine rich splicing factor 7a [Sourc...	[more]
srsf7b	7.602e-30	59.04	serine and arginine rich splicing factor 7 [Source...	[more]
srsf7b	9.297e-30	59.04	serine and arginine rich splicing factor 7 [Source...	[more]
srsf7b	1.059e-29	59.04	serine and arginine rich splicing factor 7 [Source...	[more]
srsf3a	1.063e-29	61.54	serine/arginine-rich splicing factor 3-like [Sourc...	[more]

BLAST of SMED30023281 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5


Match Name	E-value	Identity	Description
srsf3a	3.104e-30	57.29	serine and arginine rich splicing factor 3a [Sourc...	[more]
ENSPMAT00000006373.1	1.477e-29	56.99	pep scaffold:Pmarinus_7.0:GL478975:164155:166212:-...	[more]
srsf7a	2.524e-20	53.42	serine and arginine rich splicing factor 7a [Sourc...	[more]
srsf7a	2.804e-20	53.42	serine and arginine rich splicing factor 7a [Sourc...	[more]
ENSPMAT00000010377.1	2.361e-13	44.74	pep scaffold:Pmarinus_7.0:GL481287:28:8451:-1 gene...	[more]

BLAST of SMED30023281 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 2


Match Name	E-value	Identity	Description
MRD1	4.380e-7	37.33	Essential conserved small ribosomal subunit (40s) ...	[more]
NPL3	4.889e-6	31.88	RNA-binding protein; promotes elongation, regulate...	[more]

BLAST of SMED30023281 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5


Match Name	E-value	Identity	Description
EDO31304	4.983e-31	62.50	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO31305	3.474e-30	58.23	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO30175	1.414e-25	56.16	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO30178	2.336e-24	58.90	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO44646	1.361e-15	48.28	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]

BLAST of SMED30023281 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5


Match Name	E-value	Identity	Description
SRSF3	1.049e-29	54.84	serine/arginine-rich splicing factor 3 [Source:NCB...	[more]
SRSF3	1.576e-29	54.84	serine/arginine-rich splicing factor 3 [Source:NCB...	[more]
srsf7a	2.216e-29	65.38	serine/arginine-rich splicing factor 7 [Source:NCB...	[more]
srsf7a	4.289e-29	65.38	serine/arginine-rich splicing factor 7 [Source:NCB...	[more]
srsf3a	7.628e-29	58.75	serine and arginine rich splicing factor 3a [Sourc...	[more]

BLAST of SMED30023281 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5


Match Name	E-value	Identity	Description
SMESG000006493.1	2.631e-75	100.00	SMESG000006493.1	[more]
SMESG000045501.1	5.216e-15	49.30	SMESG000045501.1	[more]
SMESG000013050.1	4.105e-14	46.05	SMESG000013050.1	[more]
SMESG000013050.1	4.726e-14	47.30	SMESG000013050.1	[more]
SMESG000039881.1	3.274e-12	37.18	SMESG000039881.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence
protein sequence

transcript sequence

>SMED30023281 ID=SMED30023281|Name=SMED30023281|organism=Schmidtea mediterranea sexual|type=transcript|length=500bp
TAAAGAAGCAGAATATTTATTATAAATACATATTTGTTAAATTTACATTG
TTAGTCTTTTATTATTTAACTTCCACGAGATATTGATCGACTTCTATGTC
TTCTATGTGATGAATTTCTGATTTTTTTATCATTGTAATCATTGGAATTT
TCATATCTGCTGTTCCTACTTTGATTAGCCCAAGGCTTTGGACGGGAACG
GCCATGACTCATTTCCACTCGTACACGAACTCCACATATTACTGATCCAT
CTAGCTCTTTCACTGCATCTTCGGCATCTCTATAATCATCAAACATTACA
AAAGCAAATCCTGCTGGGTTCCTAGCAACCCATACAGATTTAATTGGACC
AAAATCTTTAAAGGCTTTTTCAATTTCTTTTTCATTTGCATCTTTCGGTA
AATCTCCTACGTAAACTTTGACTTCATCTTTGTTTTTCGACATATAGCAG
AAGATATTTAAATAGTTTTTAATATTAAAAGTATTAAAACAACTCCCCAT

protein sequence of SMED30023281-orf-1

>SMED30023281-orf-1 ID=SMED30023281-orf-1|Name=SMED30023281-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=145bp
MGSCFNTFNIKNYLNIFCYMSKNKDEVKVYVGDLPKDANEKEIEKAFKDF
GPIKSVWVARNPAGFAFVMFDDYRDAEDAVKELDGSVICGVRVRVEMSHG
RSRPKPWANQSRNSRYENSNDYNDKKIRNSSHRRHRSRSISRGS*

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0002109	X1 cell

Vocabulary: **INTERPRO**
Term	Definition
IPR035979	RBD_domain_sf
IPR000504	RRM_dom
IPR012677	Nucleotide-bd_a/b_plait_sf

Vocabulary: **molecular function**
Term	Definition
GO:0003676	nucleic acid binding
GO:0000166	nucleotide binding

InterPro

Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000504	RNA recognition motif domain	SMART	SM00360	rrm1_1	coord: 25..93 e-value: 7.7E-23 score: 91.9
IPR000504	RNA recognition motif domain	PFAM	PF00076	RRM_1	coord: 26..88 e-value: 2.9E-19 score: 68.6
IPR000504	RNA recognition motif domain	PROSITE	PS50102	RRM	coord: 24..97 score: 17.66
IPR012677	Nucleotide-binding alpha-beta plait domain superfamily	GENE3D	G3DSA:3.30.70.330		coord: 17..136 e-value: 3.1E-25 score: 90.8
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 126..141
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 94..141
None	No IPR available	PANTHER	PTHR23147	SERINE/ARGININE RICH SPLICING FACTOR	coord: 23..138
None	No IPR available	PANTHER	PTHR23147:SF18	SERINE/ARGININE-RICH SPLICING FACTOR 7	coord: 23..138
None	No IPR available	CDD	cd12373	RRM_SRSF3_like	coord: 25..97 e-value: 8.7739E-41 score: 128.892
IPR035979	RNA-binding domain superfamily	SUPERFAMILY	SSF54928	RNA-binding domain, RBD	coord: 21..118