Titin
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30023280 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 8
Homology
BLAST of Titin vs. Ensembl Human
Match: TTN (titin [Source:HGNC Symbol;Acc:HGNC:12403]) HSP 1 Score: 108.612 bits (270), Expect = 3.921e-23 Identity = 179/768 (23.31%), Postives = 304/768 (39.58%), Query Frame = 3
Query: 360 EINWFKDGKLLSAGSRFSTIF-DRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPE-EMMQEINNLDKNYETGTYHGMEEDKHA------AKTIPKFLS-----------QL-----------PNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSL--KLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLE--MNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPI------DQLNEGD-----------------SVTLT--------VKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLTVIPMVDFRPDLRHITPE--------NPFKKMAGLKKVEC---TSELNDAFKKSKPTAERIV--KI-EMGSETKSKFYKSPDVIETEKLYDQVAA 2426
+I W+KDGK + G R+ F G L I + +D G Y A + G A + + + P + + T +P E + I +L + + M + + A+ P +S QL P S ++GQ ++ + T WF DG + ++ VI + G +L I P D+G+++ VA+N G+ SSIS + ++E + + P F+E + + +++ EG+ KV + W N +I+ ++E +AL I T+ D+ Y ++ GR ++ +NV+ E +P+ E +L P+ + EI +LEP+ +Q EGD S+ L ++ P D ++ VEW + + +R+++ + G +L+ A S DSG ATNK G + I E + +V S L G QR+++LE G ++ T D +E+ P+ + P + EG A+F +V+G+P P + W ++ L+ + DG I+YL+I C +V V A NP GV + + L + DFR LR PE P K ++KV+ T+E + K + AERI K+ E E +SKF + + E Y+ + A
Sbjct: 1275 KIAWYKDGKRIKHGERYQMDFLQDGRASLRIPVVLPEDEGIYTAFASNIKGNAICS-GKLYVEPAAPLGAPTYIPTLEPVSRIRSLSPRSVSRSPIRMSPARMSPARMSPARMSPARMSPGRRLEETDESQLERLYKPVFVLKPVSFKCLEGQTARFDLKVVGRPMPE--TFWFHDGQQIVNDYTHKV-VIKEDGTQSLIIVPATPSDSGEWTVVAQNRAGR------------------------SSISVILTVEAVEHQ-----------VKPMFVEKL--KNVNIKEGSRLEMKVRATGNPNPDIV--WLKNSDIIVPHKYPKIRIEGTKGEAALKIDSTVSQDSAWYTATAINKAGR-DTTRCKVNVEVE---FAEPEP---------ERKLIIPRGTYRAKEIAA------PELEPLHLRYGQEQWEEGDLYDKEKQQKPFFKKKLTSLRLKRFGPAHFECRLTPIGDPTMVVEWLHDGKPLEAANRLRMINEFGYCSLDYGVAYSRDSGIITCRATNKYGTDHTSATLIVKDE-KSLVEESQLPEGRKGLQRIEELERMAHEGALTGV--TTDQKEKQKPDIVLYPEPVRVLEGETARFRCRVTGYPQPKVNWYLNGQLIRKSKRFRVRYDG-IHYLDIVDCKSYDTGEVKVTAENPEGVIEHKVKLEIQQREDFRSVLRR-APEPRPEFHVHEPGKLQFEVQKVDRPVDTTETKEVVKLKR--AERITHEKVPEESEELRSKFKRRTE----EGYYEAITA 1969 HSP 2 Score: 80.8777 bits (198), Expect = 1.261e-14 Identity = 131/639 (20.50%), Postives = 247/639 (38.65%), Query Frame = 3
Query: 219 LASPRRGSDHLY----DSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLE--MNISALNILYTLPSDTGSYICVLFSE---------------------HGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDL---------PSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNF--DVKPMNITAGEGGPAKFLVKVSGFPTPTLEW 2021
+A R S LY + P Q+L+ + V G+P R + + +I+W+K+ +LLS G + + D L ++ +D Y C AK+ G A ++ + ++PE + D+ P ++ L +++T +GQP + + S + L W+ +K + L+IA YPED G Y+ VA N++G+ + + N++ ++ ++ +S S + + PSFI+P+ + E + +V+ P K W+HN +++ + E ++ + I+ G Y C + H R+E E + + ++ S L + +G+ + +++N + E + P FIK++ D ++ GD TL+V V ++W+ N + + + +D +L I+ ED G+Y +A+N G+T + ++ L +S G + KD E + A ++ E + C P+F ++KP I +G PA F V G P PT+ W
Sbjct: 3175 VAGRNRSSVTLYVNAPEPPQVLQELQP-VTVQSGKPARFCAVI--SGRPQPKISWYKEEQLLSTGFKCKFLHDGQEYTLLLIEAFPEDAAVYTCEAKNDYGVATTSAS-----------LSVEVPEVV-------------------SPDQEMPVYPPAIITPLQDTVTS-EGQPARFQCRV---SGTDLKVSWYSKDKKIKPSRFFRMTQFEDTYQLEIAEAYPEDEGTYTFVASNAVGQV-SSTANLSLEGFSKFEENTSNSQWHVSLSVSFKK-----------EPLGQKPSFIQPLSSLRVHNGETVRFHARVSGIP----KPEIQWFHNQQLILPTKDVVFHFEESTGMALMLIVDAYSEHAGQYSCKAANSAGEATCAATLTVTPKAPESILHERIEQE-IEMEMKEFSSSFLSAEEEGLHSAELQLSKINETLELLSESPVYPTKFDSEKEGTGPIFIKEVSNAD-ISMGDVATLSVTV--IGIPKPKIQWFFNGVLLTPSADYKFVFDGDDHSLIILFTKLEDEGEYTCMASNDYGKT---------------ICSAYLKINSKG-EGHKDTETESAVA-----KSLEKLGGPCPPHFLKELKP--IRCAQGLPAIFEYTVVGEPAPTVTW 3733 HSP 3 Score: 76.6406 bits (187), Expect = 2.142e-13 Identity = 111/491 (22.61%), Postives = 190/491 (38.70%), Query Frame = 3
Query: 678 PKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDR--GTATLNIVSAISEDSGDYWLIATNKNGQ-TQSEKINISCFEGEKIV---------------TASSLLADSAGYQ----RLKDLEEDLASGNISTFQNTE-DVQEECL-PNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLVVPDSVCKTYNDG 2078
P+ L +L +T+ G+P + + W+K+ L G + ++D L + +PED Y+C A+N G A T + PE + +P +E Y PP+ I P+ ++ SEG PA F+ + +G L WY + +K + L I P D G+Y V + G+V S T N+ E + + +L + E + PSFI+ L + +++ G++V +V + ++W+ N+ I+ + ++ G A + IV A SE +G Y A N G+ T + + ++ E I+ ++S L A+ G +L + E L + S T+ D ++E P F + N G A V V G P P ++W + VL+ P + K DG
Sbjct: 3193 PQVLQEL-QPVTVQSGKPARFCAVISGRPQPKI--SWYKEEQLLSTGFKCKFLHDGQEYTLLLIEAFPEDAAVYTCEAKNDYGVATTSASLSVEVPE-------------------VVSPDQEMPVY--------PPAIITPL--QDTVTSEGQPARFQCRV---SGTDLKVSWYSKDKKIKPSRFFRMTQFEDTYQLEIAEAYPEDEGTYTFVASNAVGQVSS---TANLSLEGFSKFEENTSNSQWHVSLSVSF--------KKEPLGQKPSFIQPLSSL-RVHNGETVRFHARV--SGIPKPEIQWFHNQQLILPTKDVVFHFEESTGMALMLIVDAYSEHAGQYSCKAANSAGEATCAATLTVTPKAPESILHERIEQEIEMEMKEFSSSFLSAEEEGLHSAELQLSKINETLELLSESPVYPTKFDSEKEGTGPIFIKEVSNADISMGDVATLSVTVIGIPKPKIQWFFNGVLLTPSADYKFVFDG 3634 HSP 4 Score: 61.6178 bits (148), Expect = 8.810e-9 Identity = 122/523 (23.33%), Postives = 211/523 (40.34%), Query Frame = 3
Query: 294 VVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYH---GMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIY----DRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPE--ESII-------TKSNLTESSISQLKS----LENPGEEYARYNDT----SVIPMPPSFIE-------PIDPREIS---VSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGR-VESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTL--TVKVEPANDNSLYVEWYKNENHIINGSRMQISYD-RGTATLNIVSAISEDSGDYWLIATNKNGQT 1748
V EG+P R RV D L+++W+ K + F L+I +D G Y VA + G S+ A++ ++ + ++N +H + K P F+ L +SL + G+ + + + +WF + L L ++ DV++ G + I Y E G YSC A NS G+A T + +T P+ ESI+ + + E S S L + L + + ++ N+T S P+ P+ + PI +E+S +S G A VT+ K+ W+ NG ++ + + + +L IL+T D G Y C+ +++G+ + S + +N + E + +S + +LE +L P P F+K+L+PI + +G TV EPA V W+K + I ++ G+ T + EDSG Y A N G++
Sbjct: 3309 VTSEGQPARFQCRVSGTD---LKVSWYSKDKKIKPSRFFRMTQFEDTYQLEIAEAYPEDEGTYTFVASNAVGQV-SSTANLS-----------------LEGFSKFEENTSNSQWHVSLSVSFKKEPLGQKPSFIQPL-SSLRVHNGETVRFHARVSGIPKPEI--QWFHN-QQLILPTK-DVVFHFEESTGMALMLIVDAYSEHAGQYSCKAANSAGEA-TCAATLTVTPKAPESILHERIEQEIEMEMKEFSSSFLSAEEEGLHSAELQLSKINETLELLSESPVYPTKFDSEKEGTGPIFIKEVSNADISMGDVATLSVTVIGIPKPKIQ--WFFNGVLLTPSADYKFVFDGDDHSLIILFTKLEDEGEYTCMASNDYGKTICSAYLKINSKGEGHKDTETES---AVAKSLE-KLGGPCP-----------PHFLKELKPI-RCAQGLPAIFEYTVVGEPAPT----VTWFKENKQLCTSVYYTIIHNPNGSGTFIVNDPQREDSGLYICKAENMLGES 3782 HSP 5 Score: 57.7658 bits (138), Expect = 1.146e-7 Identity = 49/207 (23.67%), Postives = 88/207 (42.51%), Query Frame = 3
Query: 360 EINWFKDGKLLSAGSRFSTIFDR-GFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKF---LSQLPNSLTLIQGQPLH----IEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVT 956
E+ W+KDGK L F + G L I E D G+YVC GT+++N+ ++ Q + + ++++ + + ++ E KT PK +S P +T +Q + + ++ + W KDG ++ G + + D+G L+I D+G Y+C +NS G + SC +T
Sbjct: 26376 EVTWYKDGKKLKENGHFQFHYSADGTYELKINNLTESDQGEYVCEISGEGGTSKTNL-----------QFMGQAFKSIHEKVSKISETKKSD--QKTTESTVTRKTEPKAPEPISSKPVIVTGLQDTTVSSDSVAKFAVKATGEPRPTAIWTKDGKAITQGGKYKLSEDKGGFFLEIHKTDTSDSGLYTCTVKNSAGSV-SSSCKLT 26568 HSP 6 Score: 57.7658 bits (138), Expect = 1.334e-7 Identity = 56/209 (26.79%), Postives = 90/209 (43.06%), Query Frame = 3
Query: 1422 IHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCF-------EGEKIVTASSLLADSAGYQRLKDLE-EDLASGNISTFQNTEDVQEECLPNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEWR 2024
+H EA++ EI P+ + L+ + + EG+SVTL + + S V WY+ + I + QI++ G A L I A +EDSG + A N+ G ++ SC+ E EK TA + + + + +E D+ + S + E P F KP+ EGG F +V G P P + W+
Sbjct: 873 LHGREAKVTETARVPAPVEIPVTPPTLVSGLKNVTVI-EGESVTLECHI--SGYPSPTVTWYREDYQIESSIDFQITFQSGIARLMIREAFAEDSGRFTCSAVNEAGT-----VSTSCYLAVQVSEEFEKETTA---VTEKFTTEEKRFVESRDVVMTDTSLTEEQAGPGEPAAPYFITKPVVQKLVEGGSVVFGCQVGGNPKPHVYWK 1070 HSP 7 Score: 51.9878 bits (123), Expect = 8.109e-6 Identity = 76/336 (22.62%), Postives = 135/336 (40.18%), Query Frame = 3
Query: 978 ITKSNLTESSISQLKSLENPGEEYARYNDTSVIPM-PPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDL--------------PSFIKQLEPIDQ-LNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAIS--EDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRL-KDLEEDLASGNISTFQNTEDVQEECLPNF 1928
IT + + E L E E AR IP+ PP+ + + + ++V EG + I ++ WY +++ + I+ L I D+G + C +E G V S L VQ + + ++ ++ E + + D ++TD P FI + P+ Q L EG SV +V + +V W K+ + G R ++SY++ T +V +++ +D+G+Y ++ NK+G+T +AS+ L + A Y+ L K +E L ++ F V E P F
Sbjct: 860 ITGTTVREERFEVLHGREAKVTETARVPAPVEIPVTPPTLVSGL--KNVTVIEGESVTLECHISGYPSPTVT--WYREDYQIESSIDFQITFQSGIARLMIREAFAEDSGRFTCSAVNEAGTV-STSCYLAVQVSEE--FEKETTAVTEKFTTEEK----RFVESRDVVMTDTSLTEEQAGPGEPAAPYFITK--PVVQKLVEGGSVVFGCQV--GGNPKPHVYWKKSGVPLTTGYRYKVSYNKQTGECKLVISMTFADDAGEYTIVVRNKHGET----------------SASASLLEEADYELLMKSQQEMLYQTQVTAFVQEPKVGETA-PGF 1163
BLAST of Titin vs. Ensembl Human
Match: TTN (titin [Source:HGNC Symbol;Acc:HGNC:12403]) HSP 1 Score: 108.227 bits (269), Expect = 4.647e-23 Identity = 179/768 (23.31%), Postives = 304/768 (39.58%), Query Frame = 3
Query: 360 EINWFKDGKLLSAGSRFSTIF-DRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPE-EMMQEINNLDKNYETGTYHGMEEDKHA------AKTIPKFLS-----------QL-----------PNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSL--KLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLE--MNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPI------DQLNEGD-----------------SVTLT--------VKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLTVIPMVDFRPDLRHITPE--------NPFKKMAGLKKVEC---TSELNDAFKKSKPTAERIV--KI-EMGSETKSKFYKSPDVIETEKLYDQVAA 2426
+I W+KDGK + G R+ F G L I + +D G Y A + G A + + + P + + T +P E + I +L + + M + + A+ P +S QL P S ++GQ ++ + T WF DG + ++ VI + G +L I P D+G+++ VA+N G+ SSIS + ++E + + P F+E + + +++ EG+ KV + W N +I+ ++E +AL I T+ D+ Y ++ GR ++ +NV+ E +P+ E +L P+ + EI +LEP+ +Q EGD S+ L ++ P D ++ VEW + + +R+++ + G +L+ A S DSG ATNK G + I E + +V S L G QR+++LE G ++ T D +E+ P+ + P + EG A+F +V+G+P P + W ++ L+ + DG I+YL+I C +V V A NP GV + + L + DFR LR PE P K ++KV+ T+E + K + AERI K+ E E +SKF + + E Y+ + A
Sbjct: 1275 KIAWYKDGKRIKHGERYQMDFLQDGRASLRIPVVLPEDEGIYTAFASNIKGNAICS-GKLYVEPAAPLGAPTYIPTLEPVSRIRSLSPRSVSRSPIRMSPARMSPARMSPARMSPARMSPGRRLEETDESQLERLYKPVFVLKPVSFKCLEGQTARFDLKVVGRPMPE--TFWFHDGQQIVNDYTHKV-VIKEDGTQSLIIVPATPSDSGEWTVVAQNRAGR------------------------SSISVILTVEAVEHQ-----------VKPMFVEKL--KNVNIKEGSRLEMKVRATGNPNPDIV--WLKNSDIIVPHKYPKIRIEGTKGEAALKIDSTVSQDSAWYTATAINKAGR-DTTRCKVNVEVE---FAEPEP---------ERKLIIPRGTYRAKEIAA------PELEPLHLRYGQEQWEEGDLYDKEKQQKPFFKKKLTSLRLKRFGPAHFECRLTPIGDPTMVVEWLHDGKPLEAANRLRMINEFGYCSLDYGVAYSRDSGIITCRATNKYGTDHTSATLIVKDE-KSLVEESQLPEGRKGLQRIEELERMAHEGALTGV--TTDQKEKQKPDIVLYPEPVRVLEGETARFRCRVTGYPQPKVNWYLNGQLIRKSKRFRVRYDG-IHYLDIVDCKSYDTGEVKVTAENPEGVIEHKVKLEIQQREDFRSVLRR-APEPRPEFHVHEPGKLQFEVQKVDRPVDTTETKEVVKLKR--AERITHEKVPEESEELRSKFKRRTE----EGYYEAITA 1969 HSP 2 Score: 79.337 bits (194), Expect = 3.224e-14 Identity = 110/477 (23.06%), Postives = 190/477 (39.83%), Query Frame = 3
Query: 678 PKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRV--------ESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEM-KEDEIITDL------------------PSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNF--DVKPMNITAGEGGPAKFLVKVSGFPTPTLEW 2021
P+ L +L +T+ G+P + + W+K+ L G + ++D L + +PED Y+C A+N G A T + PE + +P +E Y PP+ I P+ ++ SEG PA F+ + +G L WY + +K + L I P D G+Y V + G+V E+ L+ + E+ I ++ + S + A E L++ + ++ K +E + L P FIK++ D ++ GD TL+V V ++W+ N + + + +D +L I+ ED G+Y +A+N G+T + ++ L +S G + KD E + A ++ E + C P+F ++KP I +G PA F V G P PT+ W
Sbjct: 3193 PQVLQEL-QPVTVQSGKPARFCAVISGRPQPKI--SWYKEEQLLSTGFKCKFLHDGQEYTLLLIEAFPEDAAVYTCEAKNDYGVATTSASLSVEVPE-------------------VVSPDQEMPVY--------PPAIITPL--QDTVTSEGQPARFQCRV---SGTDLKVSWYSKDKKIKPSRFFRMTQFEDTYQLEIAEAYPEDEGTYTFVASNAVGQVSSTANLSLEAPESILHERIEQEIEMEMKEFSSSFLSAEEEGLHSAELQLSKINETLELLSESPVYPTKFDSEKEGTGPIFIKEVSNAD-ISMGDVATLSVTV--IGIPKPKIQWFFNGVLLTPSADYKFVFDGDDHSLIILFTKLEDEGEYTCMASNDYGKT---------------ICSAYLKINSKG-EGHKDTETESAVA-----KSLEKLGGPCPPHFLKELKP--IRCAQGLPAIFEYTVVGEPAPTVTW 3608 HSP 3 Score: 57.7658 bits (138), Expect = 1.185e-7 Identity = 49/207 (23.67%), Postives = 88/207 (42.51%), Query Frame = 3
Query: 360 EINWFKDGKLLSAGSRFSTIFDR-GFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKF---LSQLPNSLTLIQGQPLH----IEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVT 956
E+ W+KDGK L F + G L I E D G+YVC GT+++N+ ++ Q + + ++++ + + ++ E KT PK +S P +T +Q + + ++ + W KDG ++ G + + D+G L+I D+G Y+C +NS G + SC +T
Sbjct: 26251 EVTWYKDGKKLKENGHFQFHYSADGTYELKINNLTESDQGEYVCEISGEGGTSKTNL-----------QFMGQAFKSIHEKVSKISETKKSD--QKTTESTVTRKTEPKAPEPISSKPVIVTGLQDTTVSSDSVAKFAVKATGEPRPTAIWTKDGKAITQGGKYKLSEDKGGFFLEIHKTDTSDSGLYTCTVKNSAGSV-SSSCKLT 26443 HSP 4 Score: 57.7658 bits (138), Expect = 1.379e-7 Identity = 85/357 (23.81%), Postives = 134/357 (37.54%), Query Frame = 3
Query: 1077 PMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMK--EDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIV---------------TASSLLADSAGYQ----RLKDLEEDLASGNISTFQNTE-DVQEECL-PNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLVVPDSVCKTYNDG 2078
P PP ++ + P ++V G PA F I K+S WY +++ T + + L ++ P D Y C +++G V + +L+V+ E+ +P EM IIT L + +EG +V + L V WY + I +++ T L I A ED G Y +A+N GQ S N+S E I+ ++S L A+ G +L + E L + S T+ D ++E P F + N G A V V G P P ++W + VL+ P + K DG
Sbjct: 3190 PEPPQVLQELQP--VTVQSGKPARFCAVISGRPQPKIS--WYKEEQLLSTGFKCKFLHDGQEYTLLLIEAFPEDAAVYTCEAKNDYG-VATTSASLSVEV--------------------PEVVSPDQEMPVYPPAIITPLQDTV--------TSEGQPARFQCRV---SGTDLKVSWYSKDKKIKPSRFFRMTQFEDTYQLEIAEAYPEDEGTYTFVASNAVGQVSS-TANLSLEAPESILHERIEQEIEMEMKEFSSSFLSAEEEGLHSAELQLSKINETLELLSESPVYPTKFDSEKEGTGPIFIKEVSNADISMGDVATLSVTVIGIPKPKIQWFFNGVLLTPSADYKFVFDG 3509 HSP 5 Score: 57.7658 bits (138), Expect = 1.403e-7 Identity = 56/209 (26.79%), Postives = 90/209 (43.06%), Query Frame = 3
Query: 1422 IHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCF-------EGEKIVTASSLLADSAGYQRLKDLE-EDLASGNISTFQNTEDVQEECLPNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEWR 2024
+H EA++ EI P+ + L+ + + EG+SVTL + + S V WY+ + I + QI++ G A L I A +EDSG + A N+ G ++ SC+ E EK TA + + + + +E D+ + S + E P F KP+ EGG F +V G P P + W+
Sbjct: 873 LHGREAKVTETARVPAPVEIPVTPPTLVSGLKNVTVI-EGESVTLECHI--SGYPSPTVTWYREDYQIESSIDFQITFQSGIARLMIREAFAEDSGRFTCSAVNEAGT-----VSTSCYLAVQVSEEFEKETTA---VTEKFTTEEKRFVESRDVVMTDTSLTEEQAGPGEPAAPYFITKPVVQKLVEGGSVVFGCQVGGNPKPHVYWK 1070 HSP 6 Score: 51.6026 bits (122), Expect = 9.438e-6 Identity = 76/336 (22.62%), Postives = 135/336 (40.18%), Query Frame = 3
Query: 978 ITKSNLTESSISQLKSLENPGEEYARYNDTSVIPM-PPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDL--------------PSFIKQLEPIDQ-LNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAIS--EDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRL-KDLEEDLASGNISTFQNTEDVQEECLPNF 1928
IT + + E L E E AR IP+ PP+ + + + ++V EG + I ++ WY +++ + I+ L I D+G + C +E G V S L VQ + + ++ ++ E + + D ++TD P FI + P+ Q L EG SV +V + +V W K+ + G R ++SY++ T +V +++ +D+G+Y ++ NK+G+T +AS+ L + A Y+ L K +E L ++ F V E P F
Sbjct: 860 ITGTTVREERFEVLHGREAKVTETARVPAPVEIPVTPPTLVSGL--KNVTVIEGESVTLECHISGYPSPTVT--WYREDYQIESSIDFQITFQSGIARLMIREAFAEDSGRFTCSAVNEAGTV-STSCYLAVQVSEE--FEKETTAVTEKFTTEEK----RFVESRDVVMTDTSLTEEQAGPGEPAAPYFITK--PVVQKLVEGGSVVFGCQV--GGNPKPHVYWKKSGVPLTTGYRYKVSYNKQTGECKLVISMTFADDAGEYTIVVRNKHGET----------------SASASLLEEADYELLMKSQQEMLYQTQVTAFVQEPKVGETA-PGF 1163
BLAST of Titin vs. Ensembl Human
Match: TTN (titin [Source:HGNC Symbol;Acc:HGNC:12403]) HSP 1 Score: 108.227 bits (269), Expect = 4.890e-23 Identity = 179/768 (23.31%), Postives = 304/768 (39.58%), Query Frame = 3
Query: 360 EINWFKDGKLLSAGSRFSTIF-DRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPE-EMMQEINNLDKNYETGTYHGMEEDKHA------AKTIPKFLS-----------QL-----------PNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSL--KLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLE--MNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPI------DQLNEGD-----------------SVTLT--------VKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLTVIPMVDFRPDLRHITPE--------NPFKKMAGLKKVEC---TSELNDAFKKSKPTAERIV--KI-EMGSETKSKFYKSPDVIETEKLYDQVAA 2426
+I W+KDGK + G R+ F G L I + +D G Y A + G A + + + P + + T +P E + I +L + + M + + A+ P +S QL P S ++GQ ++ + T WF DG + ++ VI + G +L I P D+G+++ VA+N G+ SSIS + ++E + + P F+E + + +++ EG+ KV + W N +I+ ++E +AL I T+ D+ Y ++ GR ++ +NV+ E +P+ E +L P+ + EI +LEP+ +Q EGD S+ L ++ P D ++ VEW + + +R+++ + G +L+ A S DSG ATNK G + I E + +V S L G QR+++LE G ++ T D +E+ P+ + P + EG A+F +V+G+P P + W ++ L+ + DG I+YL+I C +V V A NP GV + + L + DFR LR PE P K ++KV+ T+E + K + AERI K+ E E +SKF + + E Y+ + A
Sbjct: 1275 KIAWYKDGKRIKHGERYQMDFLQDGRASLRIPVVLPEDEGIYTAFASNIKGNAICS-GKLYVEPAAPLGAPTYIPTLEPVSRIRSLSPRSVSRSPIRMSPARMSPARMSPARMSPARMSPGRRLEETDESQLERLYKPVFVLKPVSFKCLEGQTARFDLKVVGRPMPE--TFWFHDGQQIVNDYTHKV-VIKEDGTQSLIIVPATPSDSGEWTVVAQNRAGR------------------------SSISVILTVEAVEHQ-----------VKPMFVEKL--KNVNIKEGSRLEMKVRATGNPNPDIV--WLKNSDIIVPHKYPKIRIEGTKGEAALKIDSTVSQDSAWYTATAINKAGR-DTTRCKVNVEVE---FAEPEP---------ERKLIIPRGTYRAKEIAA------PELEPLHLRYGQEQWEEGDLYDKEKQQKPFFKKKLTSLRLKRFGPAHFECRLTPIGDPTMVVEWLHDGKPLEAANRLRMINEFGYCSLDYGVAYSRDSGIITCRATNKYGTDHTSATLIVKDE-KSLVEESQLPEGRKGLQRIEELERMAHEGALTGV--TTDQKEKQKPDIVLYPEPVRVLEGETARFRCRVTGYPQPKVNWYLNGQLIRKSKRFRVRYDG-IHYLDIVDCKSYDTGEVKVTAENPEGVIEHKVKLEIQQREDFRSVLRR-APEPRPEFHVHEPGKLQFEVQKVDRPVDTTETKEVVKLKR--AERITHEKVPEESEELRSKFKRRTE----EGYYEAITA 1969 HSP 2 Score: 80.8777 bits (198), Expect = 1.102e-14 Identity = 159/690 (23.04%), Postives = 270/690 (39.13%), Query Frame = 3
Query: 219 LASPRRGSDHLY----DSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKA--------ETQSCN-------VTCHPEESIITKSNLT--ESSISQ--LKSLENPGEEY----------------ARYNDTSVIPMPPSFIEPIDPREISV-----SEG-APAYF----KVTIDPGNGQK----LSND------WYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGR--------VESEPVT-LNVQTEKSIILDPQSKGISAIHALEAELNAPKAEM-KEDEIITDL------------------PSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNF--DVKPMNITAGEGGPAKFLVKVSGFPTPTLEW 2021
+A R S LY + P Q+L+ + V G+P R + + +I+W+K+ +LLS G + + D L ++ +D Y C AK+ G A ++ + ++PE + D+ P ++ L +++T +GQP + + S + L W+ +K + L+IA YPED G Y+ VA N++G+ E Q+ + V + + S+ T ES ISQ +KS + EY A DT + S + + E+S S G AP F VT+ G+ + L +D W H G ++ RL NI L I D G Y C++ ++ G VE P + L+ + E+ I ++ + S + A E L++ + ++ K +E + L P FIK++ D ++ GD TL+V V ++W+ N + + + +D +L I+ ED G+Y +A+N G+T + ++ L +S G + KD E + A ++ E + C P+F ++KP I +G PA F V G P PT+ W
Sbjct: 3175 VAGRNRSSVTLYVNAPEPPQVLQELQP-VTVQSGKPARFCAVI--SGRPQPKISWYKEEQLLSTGFKCKFLHDGQEYTLLLIEAFPEDAAVYTCEAKNDYGVATTSAS-----------LSVEVPEVV-------------------SPDQEMPVYPPAIITPLQDTVTS-EGQPARFQCRV---SGTDLKVSWYSKDKKIKPSRFFRMTQFEDTYQLEIAEAYPEDEGTYTFVASNAVGQVSSTANLSLEVQALDRQSSGKDVRESTKSQAVADSSFTKEESKISQKEIKSFQGSSYEYEVQVFESVSQSSIHTAASVQDTQLC-HTASLSQIAESTELSKECAKESTGEAPKIFLHLQDVTVKCGDTAQFLCVLKDDSFIDVTWTHEGAKIEESERLKQSQNGNIQFLTICNVQLVDQGLYSCIVHNDCGERTTSAVLSVEGAPESILHERIEQEIEMEMKEFSSSFLSAEEEGLHSAELQLSKINETLELLSESPVYPTKFDSEKEGTGPIFIKEVSNAD-ISMGDVATLSVTV--IGIPKPKIQWFFNGVLLTPSADYKFVFDGDDHSLIILFTKLEDEGEYTCMASNDYGKT---------------ICSAYLKINSKG-EGHKDTETESAVA-----KSLEKLGGPCPPHFLKELKP--IRCAQGLPAIFEYTVVGEPAPTVTW 3800 HSP 3 Score: 57.7658 bits (138), Expect = 1.257e-7 Identity = 49/207 (23.67%), Postives = 88/207 (42.51%), Query Frame = 3
Query: 360 EINWFKDGKLLSAGSRFSTIFDR-GFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKF---LSQLPNSLTLIQGQPLH----IEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVT 956
E+ W+KDGK L F + G L I E D G+YVC GT+++N+ ++ Q + + ++++ + + ++ E KT PK +S P +T +Q + + ++ + W KDG ++ G + + D+G L+I D+G Y+C +NS G + SC +T
Sbjct: 26443 EVTWYKDGKKLKENGHFQFHYSADGTYELKINNLTESDQGEYVCEISGEGGTSKTNL-----------QFMGQAFKSIHEKVSKISETKKSD--QKTTESTVTRKTEPKAPEPISSKPVIVTGLQDTTVSSDSVAKFAVKATGEPRPTAIWTKDGKAITQGGKYKLSEDKGGFFLEIHKTDTSDSGLYTCTVKNSAGSV-SSSCKLT 26635 HSP 4 Score: 57.7658 bits (138), Expect = 1.379e-7 Identity = 56/209 (26.79%), Postives = 90/209 (43.06%), Query Frame = 3
Query: 1422 IHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCF-------EGEKIVTASSLLADSAGYQRLKDLE-EDLASGNISTFQNTEDVQEECLPNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEWR 2024
+H EA++ EI P+ + L+ + + EG+SVTL + + S V WY+ + I + QI++ G A L I A +EDSG + A N+ G ++ SC+ E EK TA + + + + +E D+ + S + E P F KP+ EGG F +V G P P + W+
Sbjct: 873 LHGREAKVTETARVPAPVEIPVTPPTLVSGLKNVTVI-EGESVTLECHI--SGYPSPTVTWYREDYQIESSIDFQITFQSGIARLMIREAFAEDSGRFTCSAVNEAGT-----VSTSCYLAVQVSEEFEKETTA---VTEKFTTEEKRFVESRDVVMTDTSLTEEQAGPGEPAAPYFITKPVVQKLVEGGSVVFGCQVGGNPKPHVYWK 1070 HSP 5 Score: 51.6026 bits (122), Expect = 9.049e-6 Identity = 76/336 (22.62%), Postives = 135/336 (40.18%), Query Frame = 3
Query: 978 ITKSNLTESSISQLKSLENPGEEYARYNDTSVIPM-PPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDL--------------PSFIKQLEPIDQ-LNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAIS--EDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRL-KDLEEDLASGNISTFQNTEDVQEECLPNF 1928
IT + + E L E E AR IP+ PP+ + + + ++V EG + I ++ WY +++ + I+ L I D+G + C +E G V S L VQ + + ++ ++ E + + D ++TD P FI + P+ Q L EG SV +V + +V W K+ + G R ++SY++ T +V +++ +D+G+Y ++ NK+G+T +AS+ L + A Y+ L K +E L ++ F V E P F
Sbjct: 860 ITGTTVREERFEVLHGREAKVTETARVPAPVEIPVTPPTLVSGL--KNVTVIEGESVTLECHISGYPSPTVT--WYREDYQIESSIDFQITFQSGIARLMIREAFAEDSGRFTCSAVNEAGTV-STSCYLAVQVSEE--FEKETTAVTEKFTTEEK----RFVESRDVVMTDTSLTEEQAGPGEPAAPYFITK--PVVQKLVEGGSVVFGCQV--GGNPKPHVYWKKSGVPLTTGYRYKVSYNKQTGECKLVISMTFADDAGEYTIVVRNKHGET----------------SASASLLEEADYELLMKSQQEMLYQTQVTAFVQEPKVGETA-PGF 1163
BLAST of Titin vs. Ensembl Human
Match: TTN (titin [Source:HGNC Symbol;Acc:HGNC:12403]) HSP 1 Score: 107.842 bits (268), Expect = 6.015e-23 Identity = 181/768 (23.57%), Postives = 305/768 (39.71%), Query Frame = 3
Query: 360 EINWFKDGKLLSAGSRFSTIF-DRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPE-EMMQEINNLDKNYETGTYHGMEEDKHA------AKTIPKFLS-----------QL-----------PNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSL--KLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLE--MNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPI------DQLNEGD-----------------SVTLT--------VKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLTVIPMVDFRPDLRHITPE--------NPFKKMAGLKKVEC---TSELNDAFKKSKPTAERIV--KI-EMGSETKSKFYKSPDVIETEKLYDQVAA 2426
+I W+KDGK + G R+ F G L I + +D G Y A + G A + + + P + + T +P E + I +L + + M + + A+ P +S QL P S ++GQ ++ + T WF DG + ++ VI + G +L I P D+G+++ VA+N G+ SSIS + ++E + V PM F+E + + +++ EG+ KV + W N +I+ ++E +AL I T+ D+ Y ++ GR ++ +NV+ E +P+ E +L P+ + EI +LEP+ +Q EGD S+ L ++ P D ++ VEW + + +R+++ + G +L+ A S DSG ATNK G + I E + +V S L G QR+++LE G ++ T D +E+ P+ + P + EG A+F +V+G+P P + W ++ L+ + DG I+YL+I C +V V A NP GV + + L + DFR LR PE P K ++KV+ T+E + K + AERI K+ E E +SKF + + E Y+ + A
Sbjct: 1321 KIAWYKDGKRIKHGERYQMDFLQDGRASLRIPVVLPEDEGIYTAFASNIKGNAICS-GKLYVEPAAPLGAPTYIPTLEPVSRIRSLSPRSVSRSPIRMSPARMSPARMSPARMSPARMSPGRRLEETDESQLERLYKPVFVLKPVSFKCLEGQTARFDLKVVGRPMPE--TFWFHDGQQIVNDYTHKV-VIKEDGTQSLIIVPATPSDSGEWTVVAQNRAGR------------------------SSISVILTVEA--------VEHQVKPM---FVEKL--KNVNIKEGSRLEMKVRATGNPNPDIV--WLKNSDIIVPHKYPKIRIEGTKGEAALKIDSTVSQDSAWYTATAINKAGR-DTTRCKVNVEVE---FAEPEP---------ERKLIIPRGTYRAKEIAAP------ELEPLHLRYGQEQWEEGDLYDKEKQQKPFFKKKLTSLRLKRFGPAHFECRLTPIGDPTMVVEWLHDGKPLEAANRLRMINEFGYCSLDYGVAYSRDSGIITCRATNKYGTDHTSATLIVKDE-KSLVEESQLPEGRKGLQRIEELERMAHEGALTGV--TTDQKEKQKPDIVLYPEPVRVLEGETARFRCRVTGYPQPKVNWYLNGQLIRKSKRFRVRYDG-IHYLDIVDCKSYDTGEVKVTAENPEGVIEHKVKLEIQQREDFRSVLRR-APEPRPEFHVHEPGKLQFEVQKVDRPVDTTETKEVVKLKR--AERITHEKVPEESEELRSKFKRRTE----EGYYEAITA 2015 HSP 2 Score: 77.0258 bits (188), Expect = 1.709e-13 Identity = 98/495 (19.80%), Postives = 174/495 (35.15%), Query Frame = 3
Query: 345 DDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAG 1829
+D + + W KDG+ L G + F+ L+I D+G YVC A + +G++ + P P F+ ++ S ++ G+ + L+ + WFK+ L + + + + LDI ED+G YSC A N +G + SC+ VI PPSFI+ ++P +I G A + + G G W+ + + +++ + + ++ L I +D G Y CV+ +E G+ G A H L+ + P+F+K+++ + L G +VTL V + V W K + I ++++S+ G A L I G Y +A N+ G +Q+ + E KI+ + L+ +AG
Sbjct: 3669 EDRKVTVTWSKDGQKLPPGKDYKICFEDKIATLEIPLAKLKDSGTYVCTASNEAGSSSCSATVTVREP-------------------------------------------PSFVKKVDPSYLMLPGESARLHCKLK--GSPVIQVTWFKNNKELSESNTVRMYFVNSEAILDITDVKVEDSGSYSCEAVNDVG---SDSCSTEI-------------------------------------VIKEPPSFIKTLEPADI--VRGTNALLQCEVS-GTGP-FEISWFKDKKQIRSSKKYRLFSQKSLVCLEIFSFNSADVGEYECVVANEVGKC----------------------GCMATHLLK-----------------EPPTFVKKVDDLIALG-GQTVTLQAAVR--GSEPISVTWMKGQEVIREDGKIKMSFSNGVAVLIIPDVQISFGGKYTCLAENEAG-SQTSVGELIVKEPAKIIERAELIQVTAG 4031 HSP 3 Score: 75.485 bits (184), Expect = 5.127e-13 Identity = 109/518 (21.04%), Postives = 196/518 (37.84%), Query Frame = 3
Query: 678 PKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVH-VAATNPLGVAKEEATLTVIPMVDFRPDLRHI 2228
P+ L +L +T+ G+P + + W+K+ L G + ++D L + +PED Y+C A+N G A T + PE + +P +E Y PP+ I P+ ++ SEG PA F+ + +G L WY + +K + L I P D G+Y V + G+V S L+++ +SI+ + +E E+ + EMK +I +++EP+ ++ G T +++ A + + +W+K I + I + ++L I+ D G+Y A+N+ G ++SC T V E P F +P ++T G AKF+ V+G P W+ D + P + + + + LE++ + + D V+ A+N G +A L +I F +L +
Sbjct: 3239 PQVLQEL-QPVTVQSGKPARFCAVISGRPQPKI--SWYKEEQLLSTGFKCKFLHDGQEYTLLLIEAFPEDAAVYTCEAKNDYGVATTSASLSVEVPE-------------------VVSPDQEMPVY--------PPAIITPL--QDTVTSEGQPARFQCRV---SGTDLKVSWYSKDKKIKPSRFFRMTQFEDTYQLEIAEAYPEDEGTYTFVASNAVGQVSST-ANLSLEAPESILHE----------RIEQEI---EMEMKAAPVIK------RKIEPL-EVALGHLAKFTCEIQSAPN--VRFQWFKAGREIYESDKCSIRSSKYISSLEILRTQVVDCGEYTCKASNEYG-------SVSC-------------------------------------TATLTVTEAYPPTFLSRPKSLTTFVGKAAKFICTVTGTPVIETIWQKDGAALSPSPNWRISDAENKHILELSNLT-IQDRGVYSCKASNKFGADICQAELIIIDKPHFIKELEPV 3653 HSP 4 Score: 70.8626 bits (172), Expect = 1.304e-11 Identity = 77/355 (21.69%), Postives = 127/355 (35.77%), Query Frame = 3
Query: 678 PKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNG 1742
P + + P +T+ G P +E + + L +WFKDG L S+ + + +L I D G YS +NS+GK+ +C V+ H + I+ PPSFI + ++++ GA + + +S W+ +G + + + N+ LN+ PSDTG Y CV + G E V L VQ + PSF + + ++ L G S+T T + V+W+K ++ G IS + L + +SGDY + TN G
Sbjct: 6644 PARIIEKPEPMTVTTGNPFALECVVTGTPE--LSAKWFKDGRELSADSKHHITFINKVASLKIPCAEMSDKGLYSFEVKNSVGKS---NCTVSVHVSDRIV----------------------------------PPSFIRKL--KDVNAILGASVVLECRV--SGSAPISVGWFQDGNEIVSGPKCQSSFSENVCTLNLSLLEPSDTGIYTCVAANVAGSDECSAV-LTVQ--------------------------------------EPPSFEQTPDSVEVL-PGMSLTFTSVIR--GTPPFKVKWFKGSRELVPGESCNISLEDFVTELELFEVQPLESGDYSCLVTNDAG 6913 HSP 5 Score: 69.3218 bits (168), Expect = 3.715e-11 Identity = 101/514 (19.65%), Postives = 180/514 (35.02%), Query Frame = 3
Query: 357 LEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPE---------------------------EMMQEINNLDKNYE---------------TGTY-------HGMEEDKHAAKTI-PKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVE-SEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQ 1745
++W+KD + L +G ++ + + + IL D G+Y C A + G+ + P V L+ + E+++E +N+ +Y G Y GM+E + P + + P S+ + G +E + + L T+WFKDG L ++ + + L I P D+G YS +N +GK SC + +D +V PPSF + +E + G+ + + +S W+H G + + + L N AL + SD+G Y C+ + G E S P+T + + PSF+++ +P+D L G +VT T V+ V W+K + ++ G R +S + A L + + SG+Y I +N+ G+
Sbjct: 7428 FHVSWYKDKRELRSGKKYKIMSENFLTSIHILNVDAADIGEYQCKATNDVGSDTCVGSIALKAPPRFVKKLSDISTVVGKEVQLQTTIEGAEPISVVWFKDKGEIVRESDNIWISYSENIATLQFSRVEPANAGKYTCQIKNDAGMQECFATLSVLEPATIVEKPESIKVTTGDTCTLECTVAGTPE--LSTKWFKDGKELTSDNKYKISFFNKVSGLKIINVAPSDSGVYSFEVQNPVGK---DSCTASLQ-------------------------------VSDRTV---PPSFTRKL--KETNGLSGSSVVMECKV--YGSPPISVSWFHEGNEISSGRKYQTTLTDNTCALTVNMLEESDSGDYTCIATNMAGSDECSAPLT----------------------------------------VREPPSFVQKPDPMDVLT-GTNVTFTSIVK--GTPPFSVSWFKGSSELVPGDRCNVSLEDSVAELELFDVDTSQSGEYTCIVSNEAGK 7855 HSP 6 Score: 67.781 bits (164), Expect = 1.222e-10 Identity = 121/577 (20.97%), Postives = 210/577 (36.40%), Query Frame = 3
Query: 180 HALEVVNQVNKRSLASPRRGSDHLYDSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHS----------------------------GTAQSNIAHVRINPESGVDYLTQ----LP--------EEMMQEINNLDKNY-ETGTYHGMEEDKHAA---------KTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNS--LYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQS 1754
++ EV N V K S + + SD P+F + L+ E G + + +V + ++WF +G +S+G ++ T L + E D+GDY C+A + + GT + + V+ P V + +P E+ + E+ D + ++G Y + ++ K KF+ +L N ++ +G+PL +E S+ W K+G+++ R ++ L+I ED G Y+C N+ GK SC+ +Q+ LE PP F++ ++P ++SV + A ++ P ++ WY ++ + N++ L +D+G YIC + G V S L VQ +K PSF +QL + + +V L V + A S + V WYK+ + + +Q S+ TATLNI +G Y ATN G S
Sbjct: 7656 YSFEVQNPVGKDSCTASLQVSDRTV-PPSFTRKLK-ETNGLSGSSVVMECKV--YGSPPISVSWFHEGNEISSGRKYQTTLTDNTCALTVNMLEESDSGDYTCIATNMAGSDECSAPLTVREPPSFVQKPDPMDVLTGTNVTFTSIVKGTPPFSVSWFKGSSELVPGDRCNVSLEDSVAELELFDVDTSQSGEYTCIVSNEAGKASCTTHLYIKAPAKFVKRL-NDYSIEKGKPLILEGTFTGTPPISVT--WKKNGINVTPSQRCNITTTEKSAILEIPSSTVEDAGQYNCYIENASGK---DSCS--------------------AQILILE-----------------PPYFVKQLEPVKVSVGDSASLQCQLAGTP----EIGVSWYKGDTKLRPTTTYKMHFRNNVATLVFNQVDINDSGEYICKAENSVGEV-SASTFLTVQEQKL-----------------------------------PPSFSRQLRDVQE-----TVGLPVVFDCAISGSEPISVSWYKDGKPLKDSPNVQTSFLDNTATLNIFKTDRSLAGQYSCTATNPIGSASS 8140 HSP 7 Score: 65.855 bits (159), Expect = 3.852e-10 Identity = 100/497 (20.12%), Postives = 165/497 (33.20%), Query Frame = 3
Query: 285 SELV-VDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYH-NGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINI 1769
+EL+ V G+P L V A L+ W+KDG+ L A ++ F L D+G Y + G++ T+ ++ D P F L N +++ G + + + WFKDG + R + + G +L+I D G+++C A NS+G ++ ++ PPSF+ P V G+ K T L+ W+ N E+V S K + S+L + SD+G+Y C + + G VE A L + + +F+++LEP L +GD+ L KV + + W+ N+ I S+ ++S+ TA L + EDSG+Y A N+ G I I
Sbjct: 4023 AELIQVTAGDPATLEYTV--AGTPELKPKWYKDGRPLVASKKYRISFKNNVAQLKFYSAELHDSGQYTFEISNEVGSSSCET-----------------------------------TFTVLDRD-----IAPFFTKPLRNVDSVVNGT---CRLDCKIAGSLPMRVSWFKDGKEIAASDRYRIAFVEGTASLEIIRVDMNDAGNFTCRATNSVGSKDSSGA----------------------------------------LIVQEPPSFV--TKPGSKDVLPGSAVCLKSTFQ--GSTPLTIRWFKGNKELVSGGSCYITKEALE-SSLELYLVKTSDSGTYTCKVSNVAGGVE----------------------------CSANL-----------FVKEPATFVEKLEPSQLLKKGDATQLACKV--TGTPPIKITWFANDREIKESSKHRMSFVESTAVLRLTDVGIEDSGEYMCEAQNEAGSDHCSSIVI 4388 HSP 8 Score: 65.855 bits (159), Expect = 3.951e-10 Identity = 96/485 (19.79%), Postives = 174/485 (35.88%), Query Frame = 3
Query: 297 VDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNG-EIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQT 1748
V E +P+ L V A L++ W KDGK + FS F+ I ++ D+G Y ++ G++ + A++R+ LD+N IP ++ + + G +H+E + + +WFKDG + ++ ++ +L++ ED +Y+C N G P ++ PG + A +P S +E ++ +G P FK+ W+ + E+V G LE + S LN+ S TG Y C + ++ G S+ T + ++T+ P F+K+LE + GDS L K+ A + V W++NE+ + + ++++ A + + + +EDSGD+ A N G T
Sbjct: 5152 VTEKDPMTLECVV--AGTPELKVKWLKDGKQIVPSRYFSMSFENNVASFRIQSVMKQDSGQYTFKVENDFGSSSCD-AYLRV----------------------LDQN------------------IPPSFTKKLTKMDKVLGSSIHMECKV--SGSLPISAQWFKDGKEISTSAKYRLVCHERSVSLEVNNLELEDTANYTCKVSNVAGDDACSGILTVKEPPSFLV-----------------KPGRQQA---------IPDSTVE-----FKAILKGTPP-FKI------------KWFKDDVELVSGPKCFIG-LEGSTSFLNLYSVDASKTGQYTCHVTNDVG---SDSCTTML------------------------------------LVTEPPKFVKKLEASKIVKAGDSSRLECKI--AGSPEIRVVWFRNEHELPASDKYRMTFIDSVAVIQMNNLSTEDSGDFICEAQNPAGST 5505 HSP 9 Score: 64.3142 bits (155), Expect = 1.225e-9 Identity = 102/496 (20.56%), Postives = 170/496 (34.27%), Query Frame = 3
Query: 255 DSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNG 1742
+ P F +LE L G+ + L +V A + ++W+K L + T F L +D+G+Y C A++ GTA S RI QLP +++ ++++ LP +LT I++ W++DG+ L+ + + L I +G YSC A N LG T SS ++L + E P F + I P I V G A F+ + Q + W + + ++ T N L IL D+G Y C ++ G+ A+L+ + + P F+K+LE +G+S+ L K+ + + V W++N++ + + +S+ A L I A +EDSGDY A N G
Sbjct: 6077 EPPYFVTELEP-LEAAVGDSVSLQCQV--AGTPEITVSWYKGDTKLRPTPEYRTYFTNNVATLVFNKVNINDSGEYTCKAENSIGTASSKTVF-RIQER-------QLPPSFARQLKDIEQTV-----------------------GLPVTLTCRLNGSAPIQVC------------WYRDGVLLRDDENLQTSFVDNVATLKILQTDLSHSGQYSCSASNPLG-----------------------TASSSARLTARE---------------PKKSPFFD-IKPVSIDVIAGESADFECHVT--GAQPMRITWSKDNKEIRPGGNYTITCVGNTPHLRILKVGKGDSGQYTCQATNDVGK----------------------------DMCSAQLS-----------VKEPPKFVKKLEASKVAKQGESIQLECKI--SGSPEIKVSWFRNDSELHESWKYNMSFINSVALLTINEASAEDSGDYICEAHNGVG 6444 HSP 10 Score: 63.5438 bits (153), Expect = 2.174e-9 Identity = 148/712 (20.79%), Postives = 224/712 (31.46%), Query Frame = 3
Query: 291 LVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHH--SDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREI---------SVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVE-------SEPVTL-------NVQTEKSIILDPQSKGISAIHALEAE------------------------LNAPKAEMK------EDEIITDL----------PSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECL-PNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLTV-----IPMVDFRP 2213
+ V GE L +V A L + W+KDGKLL++ + F L IL D G Y Q NN+ K+ T D P F +L N+ G L LE S + WF + + G++ + C L + D G+Y+CVA N G E C +E PPSF++ +P E+ SV G P FKV +W+ + R E ++ L + S +G Y CV+ + G+ EP +V+ KSIIL+ G I + LN+ K + E+E D+ P F+ +LEP++ GDSV+L +V A + V WYK + + + + ATL DSG+Y A N G S+ + F+ +QE L P+F + +I G P +++G + W D VL+ D +T ++ L+I + + +A+NPLG A A LT P D +P
Sbjct: 5713 MTVTVGETCTLECKV--AGTPELSVEWYKDGKLLTSSQKHKFSFYNKISSLRILSVERQDAGTYT-----------------------------------FQVQNNVGKSSCTAVV-----DVSDRAVPPSFTRRLKNT-----GGVLGASCILECKVAGSSPISVAWFHEKTKIVSGAKYQTTFSDNVCTLQLNSLDSSDMGNYTCVAANVAGSDE---CRAVLTVQE-------------------------------------PPSFVKEPEPLEVLPGKNVTFTSVIRGTPP-FKV------------NWFRGARELVKGDRCNIYFEDTVAELELFNIDISQSGEYTCVVSNNAGQASCTTRLFVKEPAAFLKRLSDHSVEPGKSIILESTYTGTLPISVTWKKDGFNITTSEKCNIVTTEKTCILEILNSTKRDAGQYSCEIENEAGRDVCGALVSTLEPPYFVTELEPLEAAV-GDSVSLQCQV--AGTPEITVSWYKGDTKLRPTPEYRTYFTNNVATLVFNKVNINDSGEYTCKAENSIGTASSKTV----------------------------------------FR----IQERQLPPSFARQLKDIEQTVGLPVTLTCRLNGSAPIQVCWYRDGVLLRDDENLQTSFVDNVATLKILQTDLSHSGQYSCSASNPLGTASSSARLTAREPKKSPFFDIKP 6277 HSP 11 Score: 57.7658 bits (138), Expect = 1.248e-7 Identity = 49/207 (23.67%), Postives = 88/207 (42.51%), Query Frame = 3
Query: 360 EINWFKDGKLLSAGSRFSTIFDR-GFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKF---LSQLPNSLTLIQGQPLH----IEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVT 956
E+ W+KDGK L F + G L I E D G+YVC GT+++N+ ++ Q + + ++++ + + ++ E KT PK +S P +T +Q + + ++ + W KDG ++ G + + D+G L+I D+G Y+C +NS G + SC +T
Sbjct: 32748 EVTWYKDGKKLKENGHFQFHYSADGTYELKINNLTESDQGEYVCEISGEGGTSKTNL-----------QFMGQAFKSIHEKVSKISETKKSD--QKTTESTVTRKTEPKAPEPISSKPVIVTGLQDTTVSSDSVAKFAVKATGEPRPTAIWTKDGKAITQGGKYKLSEDKGGFFLEIHKTDTSDSGLYTCTVKNSAGSV-SSSCKLT 32940 HSP 12 Score: 57.7658 bits (138), Expect = 1.280e-7 Identity = 56/209 (26.79%), Postives = 90/209 (43.06%), Query Frame = 3
Query: 1422 IHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCF-------EGEKIVTASSLLADSAGYQRLKDLE-EDLASGNISTFQNTEDVQEECLPNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEWR 2024
+H EA++ EI P+ + L+ + + EG+SVTL + + S V WY+ + I + QI++ G A L I A +EDSG + A N+ G ++ SC+ E EK TA + + + + +E D+ + S + E P F KP+ EGG F +V G P P + W+
Sbjct: 919 LHGREAKVTETARVPAPVEIPVTPPTLVSGLKNVTVI-EGESVTLECHI--SGYPSPTVTWYREDYQIESSIDFQITFQSGIARLMIREAFAEDSGRFTCSAVNEAGT-----VSTSCYLAVQVSEEFEKETTA---VTEKFTTEEKRFVESRDVVMTDTSLTEEQAGPGEPAAPYFITKPVVQKLVEGGSVVFGCQVGGNPKPHVYWK 1116 HSP 13 Score: 53.5286 bits (127), Expect = 2.216e-6 Identity = 80/374 (21.39%), Postives = 138/374 (36.90%), Query Frame = 3
Query: 1077 PMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMK--EDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRL-KDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLTV 2189
P PP ++ + P ++V G PA F I K+S WY +++ T + + L ++ P D Y C +++G V + +L+V+ E+ +P EM IIT L + +EG +V + L V WY + I +++ T L I A ED G Y +A+N GQ S N+S E I+ ++R+ +++E ++ + + + ++P+ + G AKF ++ P +W + C + I+ LEI R + + A+N G ATLTV
Sbjct: 3236 PEPPQVLQELQP--VTVQSGKPARFCAVISGRPQPKIS--WYKEEQLLSTGFKCKFLHDGQEYTLLLIEAFPEDAAVYTCEAKNDYG-VATTSASLSVEV--------------------PEVVSPDQEMPVYPPAIITPLQDTV--------TSEGQPARFQCRV---SGTDLKVSWYSKDKKIKPSRFFRMTQFEDTYQLEIAEAYPEDEGTYTFVASNAVGQVSS-TANLSLEAPESIL-----------HERIEQEIEMEMKAAPVIKRK--------------IEPLEVALGHL--AKFTCEIQSAPNVRFQWFKAGREIYESDKCSIRSSKYISSLEILRTQVVDCGEYTCKASNEYGSVSCTATLTV 3545 HSP 14 Score: 51.6026 bits (122), Expect = 9.612e-6 Identity = 76/336 (22.62%), Postives = 135/336 (40.18%), Query Frame = 3
Query: 978 ITKSNLTESSISQLKSLENPGEEYARYNDTSVIPM-PPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDL--------------PSFIKQLEPIDQ-LNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAIS--EDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRL-KDLEEDLASGNISTFQNTEDVQEECLPNF 1928
IT + + E L E E AR IP+ PP+ + + + ++V EG + I ++ WY +++ + I+ L I D+G + C +E G V S L VQ + + ++ ++ E + + D ++TD P FI + P+ Q L EG SV +V + +V W K+ + G R ++SY++ T +V +++ +D+G+Y ++ NK+G+T +AS+ L + A Y+ L K +E L ++ F V E P F
Sbjct: 906 ITGTTVREERFEVLHGREAKVTETARVPAPVEIPVTPPTLVSGL--KNVTVIEGESVTLECHISGYPSPTVT--WYREDYQIESSIDFQITFQSGIARLMIREAFAEDSGRFTCSAVNEAGTV-STSCYLAVQVSEE--FEKETTAVTEKFTTEEK----RFVESRDVVMTDTSLTEEQAGPGEPAAPYFITK--PVVQKLVEGGSVVFGCQV--GGNPKPHVYWKKSGVPLTTGYRYKVSYNKQTGECKLVISMTFADDAGEYTIVVRNKHGET----------------SASASLLEEADYELLMKSQQEMLYQTQVTAFVQEPKVGETA-PGF 1209
BLAST of Titin vs. Ensembl Human
Match: TTN (titin [Source:HGNC Symbol;Acc:HGNC:12403]) HSP 1 Score: 107.842 bits (268), Expect = 6.440e-23 Identity = 179/768 (23.31%), Postives = 304/768 (39.58%), Query Frame = 3
Query: 360 EINWFKDGKLLSAGSRFSTIF-DRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPE-EMMQEINNLDKNYETGTYHGMEEDKHA------AKTIPKFLS-----------QL-----------PNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSL--KLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLE--MNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPI------DQLNEGD-----------------SVTLT--------VKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLTVIPMVDFRPDLRHITPE--------NPFKKMAGLKKVEC---TSELNDAFKKSKPTAERIV--KI-EMGSETKSKFYKSPDVIETEKLYDQVAA 2426
+I W+KDGK + G R+ F G L I + +D G Y A + G A + + + P + + T +P E + I +L + + M + + A+ P +S QL P S ++GQ ++ + T WF DG + ++ VI + G +L I P D+G+++ VA+N G+ SSIS + ++E + + P F+E + + +++ EG+ KV + W N +I+ ++E +AL I T+ D+ Y ++ GR ++ +NV+ E +P+ E +L P+ + EI +LEP+ +Q EGD S+ L ++ P D ++ VEW + + +R+++ + G +L+ A S DSG ATNK G + I E + +V S L G QR+++LE G ++ T D +E+ P+ + P + EG A+F +V+G+P P + W ++ L+ + DG I+YL+I C +V V A NP GV + + L + DFR LR PE P K ++KV+ T+E + K + AERI K+ E E +SKF + + E Y+ + A
Sbjct: 1321 KIAWYKDGKRIKHGERYQMDFLQDGRASLRIPVVLPEDEGIYTAFASNIKGNAICS-GKLYVEPAAPLGAPTYIPTLEPVSRIRSLSPRSVSRSPIRMSPARMSPARMSPARMSPARMSPGRRLEETDESQLERLYKPVFVLKPVSFKCLEGQTARFDLKVVGRPMPE--TFWFHDGQQIVNDYTHKV-VIKEDGTQSLIIVPATPSDSGEWTVVAQNRAGR------------------------SSISVILTVEAVEHQ-----------VKPMFVEKL--KNVNIKEGSRLEMKVRATGNPNPDIV--WLKNSDIIVPHKYPKIRIEGTKGEAALKIDSTVSQDSAWYTATAINKAGR-DTTRCKVNVEVE---FAEPEP---------ERKLIIPRGTYRAKEIAAP------ELEPLHLRYGQEQWEEGDLYDKEKQQKPFFKKKLTSLRLKRFGPAHFECRLTPIGDPTMVVEWLHDGKPLEAANRLRMINEFGYCSLDYGVAYSRDSGIITCRATNKYGTDHTSATLIVKDE-KSLVEESQLPEGRKGLQRIEELERMAHEGALTGV--TTDQKEKQKPDIVLYPEPVRVLEGETARFRCRVTGYPQPKVNWYLNGQLIRKSKRFRVRYDG-IHYLDIVDCKSYDTGEVKVTAENPEGVIEHKVKLEIQQREDFRSVLRR-APEPRPEFHVHEPGKLQFEVQKVDRPVDTTETKEVVKLKR--AERITHEKVPEESEELRSKFKRRTE----EGYYEAITA 2015 HSP 2 Score: 79.337 bits (194), Expect = 2.946e-14 Identity = 110/477 (23.06%), Postives = 190/477 (39.83%), Query Frame = 3
Query: 678 PKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRV--------ESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEM-KEDEIITDL------------------PSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNF--DVKPMNITAGEGGPAKFLVKVSGFPTPTLEW 2021
P+ L +L +T+ G+P + + W+K+ L G + ++D L + +PED Y+C A+N G A T + PE + +P +E Y PP+ I P+ ++ SEG PA F+ + +G L WY + +K + L I P D G+Y V + G+V E+ L+ + E+ I ++ + S + A E L++ + ++ K +E + L P FIK++ D ++ GD TL+V V ++W+ N + + + +D +L I+ ED G+Y +A+N G+T + ++ L +S G + KD E + A ++ E + C P+F ++KP I +G PA F V G P PT+ W
Sbjct: 3239 PQVLQEL-QPVTVQSGKPARFCAVISGRPQPKI--SWYKEEQLLSTGFKCKFLHDGQEYTLLLIEAFPEDAAVYTCEAKNDYGVATTSASLSVEVPE-------------------VVSPDQEMPVY--------PPAIITPL--QDTVTSEGQPARFQCRV---SGTDLKVSWYSKDKKIKPSRFFRMTQFEDTYQLEIAEAYPEDEGTYTFVASNAVGQVSSTANLSLEAPESILHERIEQEIEMEMKEFSSSFLSAEEEGLHSAELQLSKINETLELLSESPVYPTKFDSEKEGTGPIFIKEVSNAD-ISMGDVATLSVTV--IGIPKPKIQWFFNGVLLTPSADYKFVFDGDDHSLIILFTKLEDEGEYTCMASNDYGKT---------------ICSAYLKINSKG-EGHKDTETESAVA-----KSLEKLGGPCPPHFLKELKP--IRCAQGLPAIFEYTVVGEPAPTVTW 3654 HSP 3 Score: 77.0258 bits (188), Expect = 1.611e-13 Identity = 98/495 (19.80%), Postives = 174/495 (35.15%), Query Frame = 3
Query: 345 DDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAG 1829
+D + + W KDG+ L G + F+ L+I D+G YVC A + +G++ + P P F+ ++ S ++ G+ + L+ + WFK+ L + + + + LDI ED+G YSC A N +G + SC+ VI PPSFI+ ++P +I G A + + G G W+ + + +++ + + ++ L I +D G Y CV+ +E G+ G A H L+ + P+F+K+++ + L G +VTL V + V W K + I ++++S+ G A L I G Y +A N+ G +Q+ + E KI+ + L+ +AG
Sbjct: 4596 EDRKVTVTWSKDGQKLPPGKDYKICFEDKIATLEIPLAKLKDSGTYVCTASNEAGSSSCSATVTVREP-------------------------------------------PSFVKKVDPSYLMLPGESARLHCKLK--GSPVIQVTWFKNNKELSESNTVRMYFVNSEAILDITDVKVEDSGSYSCEAVNDVG---SDSCSTEI-------------------------------------VIKEPPSFIKTLEPADI--VRGTNALLQCEVS-GTGP-FEISWFKDKKQIRSSKKYRLFSQKSLVCLEIFSFNSADVGEYECVVANEVGKC----------------------GCMATHLLK-----------------EPPTFVKKVDDLIALG-GQTVTLQAAVR--GSEPISVTWMKGQEVIREDGKIKMSFSNGVAVLIIPDVQISFGGKYTCLAENEAG-SQTSVGELIVKEPAKIIERAELIQVTAG 4958 HSP 4 Score: 70.8626 bits (172), Expect = 1.337e-11 Identity = 77/355 (21.69%), Postives = 127/355 (35.77%), Query Frame = 3
Query: 678 PKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNG 1742
P + + P +T+ G P +E + + L +WFKDG L S+ + + +L I D G YS +NS+GK+ +C V+ H + I+ PPSFI + ++++ GA + + +S W+ +G + + + N+ LN+ PSDTG Y CV + G E V L VQ + PSF + + ++ L G S+T T + V+W+K ++ G IS + L + +SGDY + TN G
Sbjct: 7571 PARIIEKPEPMTVTTGNPFALECVVTGTPE--LSAKWFKDGRELSADSKHHITFINKVASLKIPCAEMSDKGLYSFEVKNSVGKS---NCTVSVHVSDRIV----------------------------------PPSFIRKL--KDVNAILGASVVLECRV--SGSAPISVGWFQDGNEIVSGPKCQSSFSENVCTLNLSLLEPSDTGIYTCVAANVAGSDECSAV-LTVQ--------------------------------------EPPSFEQTPDSVEVL-PGMSLTFTSVIR--GTPPFKVKWFKGSRELVPGESCNISLEDFVTELELFEVQPLESGDYSCLVTNDAG 7840 HSP 5 Score: 69.3218 bits (168), Expect = 3.592e-11 Identity = 101/514 (19.65%), Postives = 180/514 (35.02%), Query Frame = 3
Query: 357 LEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPE---------------------------EMMQEINNLDKNYE---------------TGTY-------HGMEEDKHAAKTI-PKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVE-SEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQ 1745
++W+KD + L +G ++ + + + IL D G+Y C A + G+ + P V L+ + E+++E +N+ +Y G Y GM+E + P + + P S+ + G +E + + L T+WFKDG L ++ + + L I P D+G YS +N +GK SC + +D +V PPSF + +E + G+ + + +S W+H G + + + L N AL + SD+G Y C+ + G E S P+T + + PSF+++ +P+D L G +VT T V+ V W+K + ++ G R +S + A L + + SG+Y I +N+ G+
Sbjct: 8355 FHVSWYKDKRELRSGKKYKIMSENFLTSIHILNVDAADIGEYQCKATNDVGSDTCVGSIALKAPPRFVKKLSDISTVVGKEVQLQTTIEGAEPISVVWFKDKGEIVRESDNIWISYSENIATLQFSRVEPANAGKYTCQIKNDAGMQECFATLSVLEPATIVEKPESIKVTTGDTCTLECTVAGTPE--LSTKWFKDGKELTSDNKYKISFFNKVSGLKIINVAPSDSGVYSFEVQNPVGK---DSCTASLQ-------------------------------VSDRTV---PPSFTRKL--KETNGLSGSSVVMECKV--YGSPPISVSWFHEGNEISSGRKYQTTLTDNTCALTVNMLEESDSGDYTCIATNMAGSDECSAPLT----------------------------------------VREPPSFVQKPDPMDVLT-GTNVTFTSIVK--GTPPFSVSWFKGSSELVPGDRCNVSLEDSVAELELFDVDTSQSGEYTCIVSNEAGK 8782 HSP 6 Score: 67.781 bits (164), Expect = 1.192e-10 Identity = 121/577 (20.97%), Postives = 210/577 (36.40%), Query Frame = 3
Query: 180 HALEVVNQVNKRSLASPRRGSDHLYDSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHS----------------------------GTAQSNIAHVRINPESGVDYLTQ----LP--------EEMMQEINNLDKNY-ETGTYHGMEEDKHAA---------KTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNS--LYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQS 1754
++ EV N V K S + + SD P+F + L+ E G + + +V + ++WF +G +S+G ++ T L + E D+GDY C+A + + GT + + V+ P V + +P E+ + E+ D + ++G Y + ++ K KF+ +L N ++ +G+PL +E S+ W K+G+++ R ++ L+I ED G Y+C N+ GK SC+ +Q+ LE PP F++ ++P ++SV + A ++ P ++ WY ++ + N++ L +D+G YIC + G V S L VQ +K PSF +QL + + +V L V + A S + V WYK+ + + +Q S+ TATLNI +G Y ATN G S
Sbjct: 8583 YSFEVQNPVGKDSCTASLQVSDRTV-PPSFTRKLK-ETNGLSGSSVVMECKV--YGSPPISVSWFHEGNEISSGRKYQTTLTDNTCALTVNMLEESDSGDYTCIATNMAGSDECSAPLTVREPPSFVQKPDPMDVLTGTNVTFTSIVKGTPPFSVSWFKGSSELVPGDRCNVSLEDSVAELELFDVDTSQSGEYTCIVSNEAGKASCTTHLYIKAPAKFVKRL-NDYSIEKGKPLILEGTFTGTPPISVT--WKKNGINVTPSQRCNITTTEKSAILEIPSSTVEDAGQYNCYIENASGK---DSCS--------------------AQILILE-----------------PPYFVKQLEPVKVSVGDSASLQCQLAGTP----EIGVSWYKGDTKLRPTTTYKMHFRNNVATLVFNQVDINDSGEYICKAENSVGEV-SASTFLTVQEQKL-----------------------------------PPSFSRQLRDVQE-----TVGLPVVFDCAISGSEPISVSWYKDGKPLKDSPNVQTSFLDNTATLNIFKTDRSLAGQYSCTATNPIGSASS 9067 HSP 7 Score: 65.855 bits (159), Expect = 3.757e-10 Identity = 96/485 (19.79%), Postives = 174/485 (35.88%), Query Frame = 3
Query: 297 VDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNG-EIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQT 1748
V E +P+ L V A L++ W KDGK + FS F+ I ++ D+G Y ++ G++ + A++R+ LD+N IP ++ + + G +H+E + + +WFKDG + ++ ++ +L++ ED +Y+C N G P ++ PG + A +P S +E ++ +G P FK+ W+ + E+V G LE + S LN+ S TG Y C + ++ G S+ T + ++T+ P F+K+LE + GDS L K+ A + V W++NE+ + + ++++ A + + + +EDSGD+ A N G T
Sbjct: 6079 VTEKDPMTLECVV--AGTPELKVKWLKDGKQIVPSRYFSMSFENNVASFRIQSVMKQDSGQYTFKVENDFGSSSCD-AYLRV----------------------LDQN------------------IPPSFTKKLTKMDKVLGSSIHMECKV--SGSLPISAQWFKDGKEISTSAKYRLVCHERSVSLEVNNLELEDTANYTCKVSNVAGDDACSGILTVKEPPSFLV-----------------KPGRQQA---------IPDSTVE-----FKAILKGTPP-FKI------------KWFKDDVELVSGPKCFIG-LEGSTSFLNLYSVDASKTGQYTCHVTNDVG---SDSCTTML------------------------------------LVTEPPKFVKKLEASKIVKAGDSSRLECKI--AGSPEIRVVWFRNEHELPASDKYRMTFIDSVAVIQMNNLSTEDSGDFICEAQNPAGST 6432 HSP 8 Score: 65.855 bits (159), Expect = 3.986e-10 Identity = 100/497 (20.12%), Postives = 165/497 (33.20%), Query Frame = 3
Query: 285 SELV-VDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYH-NGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINI 1769
+EL+ V G+P L V A L+ W+KDG+ L A ++ F L D+G Y + G++ T+ ++ D P F L N +++ G + + + WFKDG + R + + G +L+I D G+++C A NS+G ++ ++ PPSF+ P V G+ K T L+ W+ N E+V S K + S+L + SD+G+Y C + + G VE A L + + +F+++LEP L +GD+ L KV + + W+ N+ I S+ ++S+ TA L + EDSG+Y A N+ G I I
Sbjct: 4950 AELIQVTAGDPATLEYTV--AGTPELKPKWYKDGRPLVASKKYRISFKNNVAQLKFYSAELHDSGQYTFEISNEVGSSSCET-----------------------------------TFTVLDRD-----IAPFFTKPLRNVDSVVNGT---CRLDCKIAGSLPMRVSWFKDGKEIAASDRYRIAFVEGTASLEIIRVDMNDAGNFTCRATNSVGSKDSSGA----------------------------------------LIVQEPPSFV--TKPGSKDVLPGSAVCLKSTFQ--GSTPLTIRWFKGNKELVSGGSCYITKEALE-SSLELYLVKTSDSGTYTCKVSNVAGGVE----------------------------CSANL-----------FVKEPATFVEKLEPSQLLKKGDATQLACKV--TGTPPIKITWFANDREIKESSKHRMSFVESTAVLRLTDVGIEDSGEYMCEAQNEAGSDHCSSIVI 5315 HSP 9 Score: 64.3142 bits (155), Expect = 1.174e-9 Identity = 102/496 (20.56%), Postives = 170/496 (34.27%), Query Frame = 3
Query: 255 DSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNG 1742
+ P F +LE L G+ + L +V A + ++W+K L + T F L +D+G+Y C A++ GTA S RI QLP +++ ++++ LP +LT I++ W++DG+ L+ + + L I +G YSC A N LG T SS ++L + E P F + I P I V G A F+ + Q + W + + ++ T N L IL D+G Y C ++ G+ A+L+ + + P F+K+LE +G+S+ L K+ + + V W++N++ + + +S+ A L I A +EDSGDY A N G
Sbjct: 7004 EPPYFVTELEP-LEAAVGDSVSLQCQV--AGTPEITVSWYKGDTKLRPTPEYRTYFTNNVATLVFNKVNINDSGEYTCKAENSIGTASSKTVF-RIQER-------QLPPSFARQLKDIEQTV-----------------------GLPVTLTCRLNGSAPIQVC------------WYRDGVLLRDDENLQTSFVDNVATLKILQTDLSHSGQYSCSASNPLG-----------------------TASSSARLTARE---------------PKKSPFFD-IKPVSIDVIAGESADFECHVT--GAQPMRITWSKDNKEIRPGGNYTITCVGNTPHLRILKVGKGDSGQYTCQATNDVGK----------------------------DMCSAQLS-----------VKEPPKFVKKLEASKVAKQGESIQLECKI--SGSPEIKVSWFRNDSELHESWKYNMSFINSVALLTINEASAEDSGDYICEAHNGVG 7371 HSP 10 Score: 63.5438 bits (153), Expect = 2.085e-9 Identity = 148/712 (20.79%), Postives = 224/712 (31.46%), Query Frame = 3
Query: 291 LVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHH--SDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREI---------SVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVE-------SEPVTL-------NVQTEKSIILDPQSKGISAIHALEAE------------------------LNAPKAEMK------EDEIITDL----------PSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECL-PNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLTV-----IPMVDFRP 2213
+ V GE L +V A L + W+KDGKLL++ + F L IL D G Y Q NN+ K+ T D P F +L N+ G L LE S + WF + + G++ + C L + D G+Y+CVA N G E C +E PPSF++ +P E+ SV G P FKV +W+ + R E ++ L + S +G Y CV+ + G+ EP +V+ KSIIL+ G I + LN+ K + E+E D+ P F+ +LEP++ GDSV+L +V A + V WYK + + + + ATL DSG+Y A N G S+ + F+ +QE L P+F + +I G P +++G + W D VL+ D +T ++ L+I + + +A+NPLG A A LT P D +P
Sbjct: 6640 MTVTVGETCTLECKV--AGTPELSVEWYKDGKLLTSSQKHKFSFYNKISSLRILSVERQDAGTYT-----------------------------------FQVQNNVGKSSCTAVV-----DVSDRAVPPSFTRRLKNT-----GGVLGASCILECKVAGSSPISVAWFHEKTKIVSGAKYQTTFSDNVCTLQLNSLDSSDMGNYTCVAANVAGSDE---CRAVLTVQE-------------------------------------PPSFVKEPEPLEVLPGKNVTFTSVIRGTPP-FKV------------NWFRGARELVKGDRCNIYFEDTVAELELFNIDISQSGEYTCVVSNNAGQASCTTRLFVKEPAAFLKRLSDHSVEPGKSIILESTYTGTLPISVTWKKDGFNITTSEKCNIVTTEKTCILEILNSTKRDAGQYSCEIENEAGRDVCGALVSTLEPPYFVTELEPLEAAV-GDSVSLQCQV--AGTPEITVSWYKGDTKLRPTPEYRTYFTNNVATLVFNKVNINDSGEYTCKAENSIGTASSKTV----------------------------------------FR----IQERQLPPSFARQLKDIEQTVGLPVTLTCRLNGSAPIQVCWYRDGVLLRDDENLQTSFVDNVATLKILQTDLSHSGQYSCSASNPLGTASSSARLTAREPKKSPFFDIKP 7204 HSP 11 Score: 57.7658 bits (138), Expect = 1.177e-7 Identity = 49/207 (23.67%), Postives = 88/207 (42.51%), Query Frame = 3
Query: 360 EINWFKDGKLLSAGSRFSTIFDR-GFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKF---LSQLPNSLTLIQGQPLH----IEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVT 956
E+ W+KDGK L F + G L I E D G+YVC GT+++N+ ++ Q + + ++++ + + ++ E KT PK +S P +T +Q + + ++ + W KDG ++ G + + D+G L+I D+G Y+C +NS G + SC +T
Sbjct: 33675 EVTWYKDGKKLKENGHFQFHYSADGTYELKINNLTESDQGEYVCEISGEGGTSKTNL-----------QFMGQAFKSIHEKVSKISETKKSD--QKTTESTVTRKTEPKAPEPISSKPVIVTGLQDTTVSSDSVAKFAVKATGEPRPTAIWTKDGKAITQGGKYKLSEDKGGFFLEIHKTDTSDSGLYTCTVKNSAGSV-SSSCKLT 33867 HSP 12 Score: 57.7658 bits (138), Expect = 1.228e-7 Identity = 56/209 (26.79%), Postives = 90/209 (43.06%), Query Frame = 3
Query: 1422 IHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCF-------EGEKIVTASSLLADSAGYQRLKDLE-EDLASGNISTFQNTEDVQEECLPNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEWR 2024
+H EA++ EI P+ + L+ + + EG+SVTL + + S V WY+ + I + QI++ G A L I A +EDSG + A N+ G ++ SC+ E EK TA + + + + +E D+ + S + E P F KP+ EGG F +V G P P + W+
Sbjct: 919 LHGREAKVTETARVPAPVEIPVTPPTLVSGLKNVTVI-EGESVTLECHI--SGYPSPTVTWYREDYQIESSIDFQITFQSGIARLMIREAFAEDSGRFTCSAVNEAGT-----VSTSCYLAVQVSEEFEKETTA---VTEKFTTEEKRFVESRDVVMTDTSLTEEQAGPGEPAAPYFITKPVVQKLVEGGSVVFGCQVGGNPKPHVYWK 1116 HSP 13 Score: 57.7658 bits (138), Expect = 1.347e-7 Identity = 85/357 (23.81%), Postives = 134/357 (37.54%), Query Frame = 3
Query: 1077 PMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMK--EDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIV---------------TASSLLADSAGYQ----RLKDLEEDLASGNISTFQNTE-DVQEECL-PNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLVVPDSVCKTYNDG 2078
P PP ++ + P ++V G PA F I K+S WY +++ T + + L ++ P D Y C +++G V + +L+V+ E+ +P EM IIT L + +EG +V + L V WY + I +++ T L I A ED G Y +A+N GQ S N+S E I+ ++S L A+ G +L + E L + S T+ D ++E P F + N G A V V G P P ++W + VL+ P + K DG
Sbjct: 3236 PEPPQVLQELQP--VTVQSGKPARFCAVISGRPQPKIS--WYKEEQLLSTGFKCKFLHDGQEYTLLLIEAFPEDAAVYTCEAKNDYG-VATTSASLSVEV--------------------PEVVSPDQEMPVYPPAIITPLQDTV--------TSEGQPARFQCRV---SGTDLKVSWYSKDKKIKPSRFFRMTQFEDTYQLEIAEAYPEDEGTYTFVASNAVGQVSSTA-NLSLEAPESILHERIEQEIEMEMKEFSSSFLSAEEEGLHSAELQLSKINETLELLSESPVYPTKFDSEKEGTGPIFIKEVSNADISMGDVATLSVTVIGIPKPKIQWFFNGVLLTPSADYKFVFDG 3555 HSP 14 Score: 51.6026 bits (122), Expect = 9.944e-6 Identity = 76/336 (22.62%), Postives = 135/336 (40.18%), Query Frame = 3
Query: 978 ITKSNLTESSISQLKSLENPGEEYARYNDTSVIPM-PPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDL--------------PSFIKQLEPIDQ-LNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAIS--EDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRL-KDLEEDLASGNISTFQNTEDVQEECLPNF 1928
IT + + E L E E AR IP+ PP+ + + + ++V EG + I ++ WY +++ + I+ L I D+G + C +E G V S L VQ + + ++ ++ E + + D ++TD P FI + P+ Q L EG SV +V + +V W K+ + G R ++SY++ T +V +++ +D+G+Y ++ NK+G+T +AS+ L + A Y+ L K +E L ++ F V E P F
Sbjct: 906 ITGTTVREERFEVLHGREAKVTETARVPAPVEIPVTPPTLVSGL--KNVTVIEGESVTLECHISGYPSPTVT--WYREDYQIESSIDFQITFQSGIARLMIREAFAEDSGRFTCSAVNEAGTV-STSCYLAVQVSEE--FEKETTAVTEKFTTEEK----RFVESRDVVMTDTSLTEEQAGPGEPAAPYFITK--PVVQKLVEGGSVVFGCQV--GGNPKPHVYWKKSGVPLTTGYRYKVSYNKQTGECKLVISMTFADDAGEYTIVVRNKHGET----------------SASASLLEEADYELLMKSQQEMLYQTQVTAFVQEPKVGETA-PGF 1209
BLAST of Titin vs. Ensembl Celegans
Match: ketn-1 (KETtiN (Drosophila actin-binding) homolog; Kettin [Source:UniProtKB/TrEMBL;Acc:Q9NL87]) HSP 1 Score: 237.654 bits (605), Expect = 2.236e-64 Identity = 162/517 (31.33%), Postives = 258/517 (49.90%), Query Frame = 3
Query: 255 DSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPM-PPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAI-----HALEAELNAPKAEMKEDEIITDLP----SFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISC 1775
++P F ++L ++ V E E + R+ PA+D + + W+ +G L A RF +FD G++ LD+LY D+G Y VA++ G A+SN+ V + E V YL E ++ I L+++ G E + PKFL+ LP+ + L + + +H+++ +D ++V EWF +G L GSR+ + + G+ ALDI ED+G YS A N LG+A Q C +T P I++ + ES + ++ LEN + Y R P PP+F+ P+ V EG P + + ++P N L W +G+ + R + +L+IL D G+Y C + G+ E T +I P+ + A+ +A E+ APK +E + DLP +F+KQL P Q EGD+V L +V P +DNSL EW N ++ R +S D G LNI+ ED+G Y L+ N+ G+ QS ++I+C
Sbjct: 687 EAPKFARELAGKIEVMENERVHFEARILPANDVKMTVEWYHNGNPLPAAHRFHPMFDFGYVALDLLYAYPQDSGTYTLVARNELGEARSNVELV-VGTEK-VLYLEPHHPEGLERIKELEQDRRQGI---AEVEDRTCDAAPKFLNDLPD-IQLNEHENIHVDLRATPVNDPTMVIEWFVNGRPLLTGSRVKTLNEFGFIALDIKGAIAEDSGTYSVRASNLLGEAIRQ-CVITVTPAGQILSDTQHQES-LGKINYLENLNK-YGRVEIEDKGPQEPPTFVVPLQADLGDVEEGEPIHLECQVNPINDNSLKIIWLRDGQSLPHGHRFRTFYDFGFVSLDILGFYAQDAGTYTCRAENSLGQAE---------TVATIRCAPKDAILGAVQHPRSYARIQEIEAPKPAPQE---VPDLPHQPPAFVKQLGPAIQCMEGDNVYLEAQVTPTDDNSLTYEWLVNGQPLMKAHRFVLSQDFGYIALNILYCYPEDNGTYTLVVRNRAGEAQS-TVDINC 1181 HSP 2 Score: 222.246 bits (565), Expect = 1.914e-59 Identity = 162/551 (29.40%), Postives = 269/551 (48.82%), Query Frame = 3
Query: 252 YDSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESG----VDYLTQLPEEMMQEINNLDKNYETGTYHGMEE-DKHAAKTIPKFLSQLPNSLTLIQ-GQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQ-SKGISAIHALEAELNAPKAEMKED-EIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADS---AGYQRLKDLEEDLASG 1871
+ PTF ++L + EG+ +RL R+ P +D ++ + W ++G+ L SRF + ++ LDIL +D+G Y C A S G A ++ + +G +D + + +QEI N +K +E D KT PKF++QL +SL +Q G P+H+E +E +D+ L +WF +G L G R +D GY ALDI + + +D G+++CVARNSLG+A+T N T P +I T S E S +++ LE P D P FIEP+D I E A+F+ + P L W+ +G+ + +R + +L+I +T+P D+G Y + G E + L V I+ D Q + I +E AP+A +E+ ++ P F+ QL +D + EG + P +D V+WY N N + SR + D G +L++ + ED+G+Y ++ N G+ ++ +SC T +++L D+ +QR++++E A G
Sbjct: 1484 FQPPTFTENLADKEDAQEGQSIRLECRLIPVNDPTMRVTWTRNGQPLPEASRFMPARNFDYVNLDILALYGEDSGVYTCKAVSAFGEAATSCT---VKCAAGKSLLLDTMHDASWKRVQEIENREK---------LEAVDAEPEKTAPKFVTQLNSSLGELQEGVPIHLEAQVEPTNDNQLTVQWFHNGQPLANGHRFRTRHDFGYVALDILYAFAQDTGEWACVARNSLGEAQT-IANFTVLPRGTIYTDSQHPE-SWQKIQVLEAPRAAAPEKPDAEH--DAPQFIEPLD--SIDRMEFQSAHFQTKVTPQTDPNLRIQWFKDGQPLMNSNRFKLTTDFGYISLDIAHTVPEDSGVYSVKASNAKGDAEVQ-AQLTVTGNAVILGDTQHEQSWQKIQLIE----APRAPGEEEPDVKHGPPKFVTQLHSLDGVVEGQPSHFEAQFIPFSDPKTSVQWYLNGNPLSASSRRILRNDFGLVSLDLQYTLGEDAGEYSVVVKNSEGEDRTSG-QLSC------TTRAAILGDTQHEQSWQRIQEIEAPRAPG 2004 HSP 3 Score: 219.935 bits (559), Expect = 1.258e-58 Identity = 164/575 (28.52%), Postives = 273/575 (47.48%), Query Frame = 3
Query: 168 TMAPHALEVVNQVNKRSLASPRRGSDHLYDSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTE---SSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPI-DPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKG--ISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGN 1874
++ P +L+ + Q+ A P D +++P L+ + ++EG L T+ P D S+++ W KDG+ + +R+ + D GF VLDIL+ ++ D G+Y ++SG A ++ + ++ SG+ L E+ + + L+ N +E K A P F+ L + +G H E +D+ L +W+ DG LK GSRI I GY L+I+ YPEDNG+Y C A N +G+A T S +TC P+E II+ + L E ++ ++ +E P AR + PP F + P E+ EG A+ + + P +L +W+HNG+ V +R+ + L + P D+G++ C ++HG E L V + + QS I LE ++ PK ++ + PSF + L + QLNE D+ +EP D +L V+W N + I +R+ + + G ATL I I+ D+G+Y +ATN G S G+ V +S+ + Q+L D E++ G+
Sbjct: 2645 SLQPQSLDRIRQIEAGKPA-PEERPDQQFEAPAIVNALQVQGALEEGGSAHLQTQFTPVADPSIKVEWLKDGQPIFHSNRYKMVHDFGFAVLDILHLLKHDAGEYTFRVSNNSGEASTSTS-FEVSESSGL-ILQPQNEQKAKAVEILEDNLRRRPEEIEQELKEAT---PVFIEPLSAPVETEEGGRAHFTARYEPVNDNQLQVQWYHDGRPLKNGSRIKTINSFGYVVLEISPTYPEDNGEYICRAVNRVGEAVT-STKLTCTPKEGIISATQLPERMANAGRRIAEIEAP--RPAREDAPDADHGPPKFTSALAGPPEL--QEGQQAHLECQVTPVADPRLVIEWFHNGQPVNHTNRMKAIHDFGFVVLQLTPAEPQDSGTWTCRATNQHGSDEVS-TELKVVGGGGVSYEWQSTAERKERITELEDWIHRPKEDLNLPAVDYPAPSFSQGLTDLGQLNEADATAFVCVLEPIGDPTLRVQWEHNGHPIPYSNRISCTNEFGVATLLIKHLIAADAGEYKCVATNVKGSATS--------VGKIAVESSTQIDAPQVVQQLVDSVENILEGD 3199 HSP 4 Score: 218.394 bits (555), Expect = 4.241e-58 Identity = 189/654 (28.90%), Postives = 299/654 (45.72%), Query Frame = 3
Query: 261 PTFQQDLESEL-VVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEM--MQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPN-SLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQL-EPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSA---GYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMN-ITAGEGGPAKFLVKVSGFPTPTL--EWRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLTV 2189
PT + L + + V E + L L +V P DD +L W +G L A SR+ + D GF+ LDI Y I +D+G Y V + +G A+++ +++ + T PE + ++E+ L D AA P F QL + L +GQ +H++ ++ +D SL EWF DG L GSRI I+D GY L+ +PED G Y+C A N +G+A T + C P +I ++ ESS +++ +EN +E R + PP+F E + +E EG + + P N + W NG+ + SR + L+IL D+G Y C S G + T+ KS++LD S E E N K E + E P F+ QL + +L EG + L +VEP NDN L V+W+ N + NG R + +D G L+I+ A ++D+G++ +A N G+ Q+ I+ F ++ ++ DS +Q+++ LE A+ D + + P F ++P++ I E A F KV+ P L +W D ++ + K D L+I V A+N G A+ +A LTV
Sbjct: 1221 PTIAKPLPATIDSVHESQTLHLEAQVTPIDDNTLRYEWLFNGNPLKASSRYRVLNDFGFVSLDIDYIIAEDSGKYTLVVYNSAGRAETS-CEFQVDRLKSILSDTAHPESLRRIREMEQLQP--------AKPSDDDAAAQPPVFTQQLTGPTEPLKEGQSVHMDCVVQPINDPSLRIEWFHDGRPLMFGSRIRTIHDFGYVGLEFLHIHPEDTGTYTCKATNLIGEA-TTDIFLECKPRRNIYLDTH-HESSWQKIQEIENRVDE--REPTPELTFQPPTFTENLADKE-DAQEGQSIRLECRLIPVNDPTMRVTWTRNGQPLPEASRFMPARNFDYVNLDILALYGEDSGVYTCKAVSAFGEAATS-CTVKCAAGKSLLLDTMHDA-SWKRVQEIE-NREKLEAVDAEPEKTAPKFVTQLNSSLGELQEGVPIHLEAQVEPTNDNQLTVQWFHNGQPLANGHRFRTRHDFGYVALDILYAFAQDTGEWACVARNSLGEAQT----IANF---TVLPRGTIYTDSQHPESWQKIQVLEAPRAAA-----PEKPDAEHDA-PQF-IEPLDSIDRMEFQSAHFQTKVTPQTDPNLRIQWFKDGQPLMNSNRFKLTTDFGYISLDIAHTVPEDSGVYSVKASNAKGDAEVQAQLTV 1843 HSP 5 Score: 198.364 bits (503), Expect = 1.280e-51 Identity = 175/657 (26.64%), Postives = 307/657 (46.73%), Query Frame = 3
Query: 15 NGAIFKCITRHKYSFIFNLDFIAMTTQESMEY-----NNNSNFNSVPDEGVIPNGT----TMAPHALEVVNQVNKRSLASPRRGSDHLYDSPTFQQDLESELVVD--EGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESII--TKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILD---PQS-KGISAIHALE--AELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNF 1928
NG F+ TRH + ++ LD + Q++ E+ N+ ++ + V+P GT + P + + + + A+P + D +D+P F + L+S +D E + T+V P D +L I WFKDG+ L +RF D G+I LDI +T+ +D+G Y A + G A+ A + + + + TQ E+ Q+I ++ G EE+ PKF++QL + +++GQP H E SD +W+ +G L SR + D G +LD+ + ED G+YS V +NS G+ T S ++C +I+ T+ + I ++++ PG E + PSF++P+ + EG+ A + + P N L W++N + + + ++ + +L I TG Y C +++ G + + VQ + ++LD P S + I + A++ A L+AP+ E ++ P +++ E + + EG +VTL VEP+ D L +EW N + N +R + Y+ G A L IV + DSG Y A N G+ S ++ EKI+ S ++R+++LE + E++ ++ PNF
Sbjct: 1665 NGHRFR--TRHDFGYV-ALDILYAFAQDTGEWACVARNSLGEAQTIANFTVLPRGTIYTDSQHPESWQKIQVLEAPRAAAPEK-PDAEHDAPQFIEPLDS---IDRMEFQSAHFQTKVTPQTDPNLRIQWFKDGQPLMNSNRFKLTTDFGYISLDIAHTVPEDSGVYSVKASNAKGDAEVQ-AQLTVTGNAVILGDTQH-EQSWQKIQLIEAPRAPG-----EEEPDVKHGPPKFVTQLHSLDGVVEGQPSHFEAQFIPFSDPKTSVQWYLNGNPLSASSRRILRNDFGLVSLDLQYTLGEDAGEYSVVVKNSEGEDRT-SGQLSCTTRAAILGDTQHEQSWQRIQEIEAPRAPGAE-----PEGPVYEKPSFVQPLQSVG-DLPEGSVALLEARLVPVNDPNLRVQWFYNDQPLMESNWISTSNDFGCVSLRIAPVYARHTGVYSCKAWNDSGNAVTS-ANVGVQGSEGLLLDTSHPASLQKIQELEAIDKYARLDAPEREYEK-------PQWVQGFENYENVGEGQTVTLHGLVEPSGDPHLRLEWLLNGTPLRNANRFRQEYEFGNAILTIVHVLPHDSGVYTCRAWNTQGEA-STSATVTTAGYEKILYDSQ---HPVSWERIQELEAP------KIVEEIEEIVQKEKPNF 2282 HSP 6 Score: 197.978 bits (502), Expect = 1.488e-51 Identity = 189/752 (25.13%), Postives = 321/752 (42.69%), Query Frame = 3
Query: 42 RHKYSFIF-NLDFIAMTTQESMEYNNNS-----------NFNSVPDEGVIPNGTTMAPHALEVVNQVNKRSLASPRRGSDHLYDSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRIN-PESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSL-TLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADS---AGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAG---EGGPAKFLVKVSGFPTP----TLEWRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLTVIPMVDFRPDLRH 2225
R Y F F +LD + Q++ Y + P + ++ G P + + ++ A P+ D + P F + L + EG+ + L +V P DD SL W +G+ L RF D G+I L+ILY +DNG Y V ++ +G AQS V IN +G ++ + I L+ + E K +P LP ++ ++ + Q LH+E + D++L EW +G LK SR V+ D G+ +LDI + ED+G Y+ V NS G+AET SC +SI++ + E S+ +++ +E + A+ +D PP F + + + EG + + P N L +W+H+G + SR+ + L L+ P DTG+Y C + G ++ + L + ++I LD + S+ ++ N E+ P+F + L + EG S+ L ++ P ND ++ V W +N + SR + + L+I++ EDSG Y A + G+ + + C G+ SLL D+ A ++R++++E N + + E+ P F V +N + G EG P +V PT T++W + + +T +D L+I + A N LG A+ A TV+P D +H
Sbjct: 1007 RTFYDFGFVSLDILGFYAQDAGTYTCRAENSLGQAETVATIRCAPKDAIL--GAVQHPRSYARIQEIEAPKPA-PQEVPDLPHQPPAFVKQLGPAIQCMEGDNVYLEAQVTPTDDNSLTYEWLVNGQPLMKAHRFVLSQDFGYIALNILYCYPEDNGTYTLVVRNRAGEAQST---VDINCGHTGGNFTDSFHPNSLHRIAELETPIQRA-----EPLPDKEKEVPTIAKPLPATIDSVHESQTLHLEAQVTPIDDNTLRYEWLFNGNPLKASSRYRVLNDFGFVSLDIDYIIAEDSGKYTLVVYNSAGRAET-SCEFQVDRLKSILSDTAHPE-SLRRIREMEQ--LQPAKPSDDDAAAQPPVFTQQLTGPTEPLKEGQSVHMDCVVQPINDPSLRIEWFHDGRPLMFGSRIRTIHDFGYVGLEFLHIHPEDTGTYTCKATNLIGEATTD-IFLECKPRRNIYLDTHHE--SSWQKIQEIENRVDEREPTPELTFQPPTFTENLADKEDAQEGQSIRLECRLIPVNDPTMRVTWTRNGQPLPEASRFMPARNFDYVNLDILALYGEDSGVYTCKAVSAFGEAAT-SCTVKCAAGK------SLLLDTMHDASWKRVQEIE------NREKLEAVDAEPEKTAPKF-VTQLNSSLGELQEGVPIHLEAQVE--PTNDNQLTVQWFHNGQPLANGHRFRTRHDFGYVALDILYAFAQDTGEWACVARNSLGEAQTIANFTVLPRGTIYTDSQH 1724 HSP 7 Score: 187.578 bits (475), Expect = 3.377e-48 Identity = 172/663 (25.94%), Postives = 282/663 (42.53%), Query Frame = 3
Query: 258 SPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGE-EYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAE---LNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEG-EKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNF--DVKPMNITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLTVIPMVDFRPDLRH 2225
SP F+ L+S+ +V EG+ RL + P +D +L I W +GK + A SR T D G L+I D G+Y VA + G A R++ V ++ Y++ G + +P F S L S + +GQ +H+E L +D L W DG L + G+ +LDI D+G YSC A N LG++E Q+ + I+ KS+L + + +E+ E ++A + + P F+ I P G ++F+ I P N L W +G+ + +R+ + +L++ T P D G Y CVLF+ HG+ +S V + + + + I L++ + E E+ + P F ++L ++ E + V ++ PAND + VEWY N N + R +D G L+++ A +DSG Y L+A N+ G+ +S N+ G EK++ + G +R+K+LE+D G ED + P F D+ + + E PT +EW ++ ++ S KT N+ L+I V A+N LG A + +TV P D +H
Sbjct: 297 SPKFEGSLQSQQLV-EGQSARLEIKYTPVEDPNLRIAWLLNGKGILASSRIVTFTDFGIAALEINPVNVFDQGEYTVVAVNPLGEA-------RVSANIAVIGHGSFIQQQQSGSQFGGTAYQS---KGAQAPAGTHLDLPNFHSDL-RSQEVFEGQQIHLETKLTPINDPDLRVVWLLDGNELPSNDKYRQTLSHGFASLDIPQTSSNDSGLYSCRAFNKLGESENQATII-------IVPKSDLQQFEQHRQLDVEDVREIQFAHSSQD----LTPKFLSQIQPFHCEQELGR-SFFEARIQPINDPTLRVSWLKDGQPLPNANRIQIFQNFGVVSLSLHPTYPEDAGVYTCVLFNSHGQAQSSAELTTVWIDTLQLDSKHADSLPIIGYLDSHQIHIGPQSVERPEEFNSLEAPKFARELAGKIEVMENERVHFEARILPANDVKMTVEWYHNGNPLPAAHRFHPMFDFGYVALDLLYAYPQDSGTYTLVARNELGEARS---NVELVVGTEKVLYLEPHHPE--GLERIKELEQDRRQG----IAEVEDRTCDAAPKFLNDLPDIQLNEHENIHVDLRATPVNDPTMVIEWFVNGRPLLTGSRVKTLNEFGFIALDIKGAIAEDSGTYSVRASNLLGEAIRQCVITVTPAGQILSDTQH 926 HSP 8 Score: 186.808 bits (473), Expect = 5.252e-48 Identity = 197/808 (24.38%), Postives = 336/808 (41.58%), Query Frame = 3
Query: 15 NGAIFKCITRHKYSFIF--------NLDFIAMTTQESMEYNNNSNFNSVPDEGVIPNGTTMAPHALEVVNQVNKRSLASPRR--GSDHLYDSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKT---IPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAEL---NAPKAEMKEDEIITD----LPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQ--EECLPNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLVVPDSVCKTYNDGSINYLEI--NRCSELGDIKVHVAATNPLGVAKEEATLTVIPMVDFRPDLRHITPENPFKKMAGLKKVECTSE------LNDAFKKS-KPTAERIVKIE 2345
NG+ K I Y + N ++I E ++ P EG+I + T + ++ + P R D + P F L + EG+ L +V P D L I WF +G+ ++ +R I D GF+VL + D+G + C A + G+ + + +++ GV Y Q E + I L+ H +ED + P F L + L + LE D +L +W +G + +RI + G L I D G+Y CVA N G A +S+ ++ ++ I P + +D E ++ EG + + + P N +L +W NG + SR E +L+ILY P D G Y V+ ++ G S+ + V S+ Q+ G + +LE+ + K ++ ++I + P F QL+ I L EG+ +V P ND L VEW+K++ ++ G R + + D G A L+++ A+ +D+G+Y +ATN++GQT ++C ++T S + R+ ++++D + NI + VQ ++ P F + N+ E PA+F V+G+P P + W I+ + K DG ++YL + +R S+ G+ V A N G ATL + DFR + P N FK L++ E + L AF+ + KP A++++ +E
Sbjct: 2854 NGSRIKTINSFGYVVLEISPTYPEDNGEYICRAVNRVGEAVTSTKLTCTPKEGII-SATQLPERMANAGRRIAEIEAPRPAREDAPDADHGPPKFTSALAGPPELQEGQQAHLECQVTPVADPRLVIEWFHNGQPVNHTNRMKAIHDFGFVVLQLTPAEPQDSGTWTCRATNQHGSDEVS-TELKVVGGGGVSYEWQSTAERKERITELED-----WIHRPKEDLNLPAVDYPAPSFSQGLTDLGQLNEADATAFVCVLEPIGDPTLRVQWEHNGHPIPYSNRISCTNEFGVATLLIKHLIAADAGEYKCVATNVKGSA-----------------------TSVGKIA-----------VESSTQIDAPQVVQQLVDSVE-NILEGDSIHLECRVTPINDPRLHVEWLRNGAPLPEASRFKPTFEFGFVSLDILYAYPEDNGDYELVVRNDKGEARSK-TKITVLPRPSLDYTSQTHG-NQQDSLESHFKQHSQAKLQLTANDIYNESDKRAPEFRTQLQNIGVL-EGEFCRFETQVAPINDPYLKVEWFKDKKPVLLGHRFRSTLDFGFACLDLLYALPDDTGEYHCVATNRHGQTMI-SAKLACQGASHVITDSQMPQGL----RVSNVKKD--NKNIYWSEQGGAVQPKQKQAPQFTIPLRNLQVTENQPARFECAVTGYPRPKVTWFINGNQCLHGHRFKLNFDG-LHYLTVSKSRISDAGE--VVAIARNTEGETISTATLDIFQNDDFR--QTKLRPAN-FKTSDELRERELQWQRDTLGSLGPAFEAAPKPDAQKLMHVE 3603 HSP 9 Score: 186.808 bits (473), Expect = 5.440e-48 Identity = 146/580 (25.17%), Postives = 259/580 (44.66%), Query Frame = 3
Query: 249 LYDSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGV--DYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQS-KGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAGEGGPAKF 1979
+YD P ++ + +EG+ + + P +D L++ W ++G L+ GS+++ D G LD+ YT +D G Y + G A S+ A ++ + + + D Q + +QEI + +EE K P+F+ QL + +L++ QP H E +E D +L WF +G + SR+ +I D G+ +DIA P D+G++ CVA+N+ G+A + + +E +I + +L S+ +++ +E D P+ + + + ++ EG A+ + P + +W +G+ + +R + + L+IL+ L D G Y + + G S + V +IL PQ+ + A+ LE L + E E E+ P FI+ L + EG T + EP NDN L V+WY + + NGSR++ G L I ED+G+Y A N+ G+ + ++C E I++A+ L A R I+ + +E+ P+ D P T+ GP +
Sbjct: 2408 VYDHPAITTQIDDK-ECNEGDHVHFEALITPVNDPRLQVQWIRNGVPLAHGSKYAIQQDFGICTLDVAYTYPEDEGVYQLRIWNPEGEAVSS-ATLKCHGKDAILGDVQHQESWKRIQEIEAPKQK--------LEEADPEPKGPPRFIQQLTSPGSLVENQPAHFEATVEPVDDPTLTINWFLNGEPMSASSRVKMINDFGWVIMDIAQTEPRDSGEWKCVAKNAAGEA-VSTATIEVQGKE-VILQDSLQPQSLDRIRQIEAGKPAPEERPDQQF--EAPAIVNALQ-VQGALEEGGSAHLQTQFTPVADPSIKVEWLKDGQPIFHSNRYKMVHDFGFAVLDILHLLKHDAGEYTFRVSNNSGEA-STSTSFEVSESSGLILQPQNEQKAKAVEILEDNLRR-RPEEIEQELKEATPVFIEPLSAPVETEEGGRAHFTARYEPVNDNQLQVQWYHDGRPLKNGSRIKTINSFGYVVLEISPTYPEDNGEYICRAVNRVGEAVT-STKLTCTPKEGIISATQLPERMANAGR-----------RIAEIEAPRPAREDA-PDADHGPPKFTSALAGPPEL 2957 HSP 10 Score: 154.451 bits (389), Expect = 8.939e-38 Identity = 140/509 (27.50%), Postives = 223/509 (43.81%), Query Frame = 3
Query: 243 DHLYDSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALE----AELNAPKAEMKEDEIIT-DLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQS 1754
D + P F L S V EG+P + P D + W+ +G LSA SR D G + LD+ YT+ +D G+Y V K+ G +++ + + + TQ E+ Q I ++ G E + P F+ L + L +G +E L +D +L +WF + L + I D G +L IA Y G YSC A N G A T S NV E ++ ++ +S+ +++ LE ++YAR + P +++ + E +V EG ++P L +W NG ++ +R + E + L I++ LP D+G Y C ++ G + EK I+ D Q H + EL APK + +EI+ + P+F+ QLE + + EG + L +PA D L V W KN + +Q ++ G ATL+I SA + +G Y L TN G+ S
Sbjct: 1877 DVKHGPPKFVTQLHSLDGVVEGQPSHFEAQFIPFSDPKTSVQWYLNGNPLSASSRRILRNDFGLVSLDLQYTLGEDAGEYSVVVKNSEGEDRTS-GQLSCTTRAAILGDTQH-EQSWQRIQEIEAPRAPGA-----EPEGPVYEKPSFVQPLQSVGDLPEGSVALLEARLVPVNDPNLRVQWFYNDQPLMESNWISTSNDFGCVSLRIAPVYARHTGVYSCKAWNDSGNAVT-SANVGVQGSEGLLLDTS-HPASLQKIQELEAI-DKYARLDAPEREYEKPQWVQGFENYE-NVGEGQTVTLHGLVEPSGDPHLRLEWLLNGTPLRNANRFRQEYEFGNAILTIVHVLPHDSGVYTCRAWNTQGEASTSATVTTAGYEK-ILYDSQ-------HPVSWERIQELEAPKIVEEIEEIVQKEKPNFLTQLESAENVPEGVPLHLESTFQPARDPELKVVWQKNGQPLGASQLVQTKHELGWATLDISSANEDHNGVYTLTITNSEGEAVS 2366 HSP 11 Score: 118.627 bits (296), Expect = 1.138e-26 Identity = 127/506 (25.10%), Postives = 211/506 (41.70%), Query Frame = 3
Query: 255 DSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQS-CNVTCHPEESIITKSNLTESSIS-QLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEII---TDL-PSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQS 1754
++P F +S + D G+ ++L + A +G + WFKD + +S+G ++ L I + G Y C A + GT R+ S + EM+ + +DK + T +D A+K+ PKF L S L++GQ +EI D +L W +G + SRI D G AL+I D G+Y+ VA N LG+A + V H +S + Q K + P + P+F D R V EG + + + P N L W +G + + + L ++L+I T +D+G Y C F++ G E+ + +II+ P+S + E +++E + DL P F+ Q++P E +++P ND +L V W K+ + N +R+QI + G +L++ ED+G Y + N +GQ QS
Sbjct: 165 EAPEFTGKFQSTTLYD-GDSVKLYCKA--AGEG-VSFKWFKDNEPISSGGSYAVDNKGNETTLHINNATMKEGGWYRCDATNKHGT---TTLKGRVVVNSRQKFNGPAHREMIT-LRKVDKVERSRTPVNQLQDVSASKSSPKFEGSL-QSQQLVEGQSARLEIKYTPVEDPNLRIAWLLNGKGILASSRIVTFTDFGIAALEINPVNVFDQGEYTVVAVNPLGEARVSANIAVIGHGSFIQQQQSGSQFGGTAYQSKGAQAPAGTHLDL---------PNFHS--DLRSQEVFEGQQIHLETKLTPINDPDLRVVWLLDGNELPSNDKYRQTLSHGFASLDIPQTSSNDSGLYSCRAFNKLGESEN---------QATIIIVPKSD----LQQFEQHRQLDVEDVREIQFAHSSQDLTPKFLSQIQPFHCEQELGRSFFEARIQPINDPTLRVSWLKDGQPLPNANRIQIFQNFGVVSLSLHPTYPEDAGVYTCVLFNSHGQAQS 637 HSP 12 Score: 81.2629 bits (199), Expect = 2.816e-15 Identity = 109/509 (21.41%), Postives = 192/509 (37.72%), Query Frame = 3
Query: 666 AKTIPKFLSQLPNSLTLIQGQPLHIEIF-LEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLTV 2189
+K P +SQ L +QG+ F +E + + W KDG +K R + +L I G+Y+ N+ G E+ + N+T P + + + AR ND + + + PA F I G K + W+ +G+ + R E L + +D G Y V + G + LNV +K+ KG P+ E P F Q++PI ++EG + + D + + WY+N + + +IS +G A L I +A +ED +Y + A+N G+ S A+ +L +G + + ++ G +++Q+++ + P+F K +I+A +G KF +V G P PT++W+ + + K DG LE+ R + + + N G A +A LT+
Sbjct: 3902 SKQAPPTISQ---QLKPLQGELGKAAKFVIEFAGAAPVKVTWLKDGKEIKSTFRTLITTTPTNSSLHIGRLENSHAGEYTVRLENAAGTVESLA-NLTVAPPTTQGKAPDFS-----------------ARLND------------------LRIQQNGPAEFSCQI--GGEPKPTIQWFKDGQPLPNDDRFQVVEEGGAYKLKFSNIISTDAGIYEIVAKNGVGEARCK-ARLNVNLQKT------GKGAE---------EGPRYEA---------PRFTSQIQPI-VVDEGKGAQFSAQFSGFPDPT--IRWYRNNEPVKHADGYEISQSKGEAILRISAARNEDVAEYKVEASNPAGKASS--------------VANLVLTPRSG----RIAKSTISRGGSASYQSSDKAAADS-PHFIAKLSDISARQGHTVKFSAEVDGNPEPTVQWQFKGKPISASNNVKISRDGKRAILELARVTPDSAGEYQIVIRNDKGAATSQAKLTL 4322 HSP 13 Score: 58.5362 bits (140), Expect = 2.897e-8 Identity = 57/224 (25.45%), Postives = 93/224 (41.52%), Query Frame = 3
Query: 1530 LNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQ-EECLPNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLE--WRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLTVI 2192
L +GDSV L K A + +W+K+ I +G + TL+I +A ++ G Y ATNK+G T +G +V + A + + + D + + +DV + P F+ + EG A+ +K + P L W ++ ++ S T+ D I LEIN + + V A NPLG A+ A + VI
Sbjct: 178 LYDGDSVKLYCK---AAGEGVSFKWFKDNEPISSGGSYAVDNKGNETTLHINNATMKEGGWYRCDATNKHGTT--------TLKGRVVVNSRQKFNGPAHREMITLRKVDKVERSRTPVNQLQDVSASKSSPKFEGSLQSQQLVEGQSARLEIKYTPVEDPNLRIAWLLNGKGILASSRIVTFTDFGIAALEINPVNVFDQGEYTVVAVNPLGEARVSANIAVI 390 HSP 14 Score: 58.5362 bits (140), Expect = 2.947e-8 Identity = 86/371 (23.18%), Postives = 133/371 (35.85%), Query Frame = 3
Query: 678 PKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQS-CNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTI----DPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSE-KINIS 1772
P F ++L N L + Q P E + + +WFKDG L R V+ + G L + D G Y VA+N +G+A ++ NV L+ EE RY P F I P I V EG A F DP + WY N E VK + L I D Y +VE+ + +++L P+S I+ + D+ D P FI +L I +G +V + +V+ + V+W I + ++IS D A L + + +G+Y ++ N G S+ K+ +S
Sbjct: 4006 PDFSARL-NDLRIQQNGP--AEFSCQIGGEPKPTIQWFKDGQPLPNDDRFQVVEEGGAYKLKFSNIISTDAGIYEIVAKNGVGEARCKARLNVN--------------------LQKTGKGAEEGPRYE-------APRFTSQIQP--IVVDEGKGAQFSAQFSGFPDP------TIRWYRNNEPVKHADGYEISQSKGEAILRISAARNEDVAEY---------KVEASNPAGKASSVANLVLTPRSGRIAKS---TISRGGSASYQSSDKAAADSPHFIAKLSDIS-ARQGHTVKFSAEVD--GNPEPTVQWQFKGKPISASNNVKISRDGKRAILELARVTPDSAGEYQIVIRNDKGAATSQAKLTLS 4323
BLAST of Titin vs. Ensembl Celegans
Match: ketn-1 (KETtiN (Drosophila actin-binding) homolog; Kettin [Source:UniProtKB/TrEMBL;Acc:Q9NL87]) HSP 1 Score: 237.269 bits (604), Expect = 2.296e-64 Identity = 162/517 (31.33%), Postives = 258/517 (49.90%), Query Frame = 3
Query: 255 DSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPM-PPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAI-----HALEAELNAPKAEMKEDEIITDLP----SFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISC 1775
++P F ++L ++ V E E + R+ PA+D + + W+ +G L A RF +FD G++ LD+LY D+G Y VA++ G A+SN+ V + E V YL E ++ I L+++ G E + PKFL+ LP+ + L + + +H+++ +D ++V EWF +G L GSR+ + + G+ ALDI ED+G YS A N LG+A Q C +T P I++ + ES + ++ LEN + Y R P PP+F+ P+ V EG P + + ++P N L W +G+ + R + +L+IL D G+Y C + G+ E T +I P+ + A+ +A E+ APK +E + DLP +F+KQL P Q EGD+V L +V P +DNSL EW N ++ R +S D G LNI+ ED+G Y L+ N+ G+ QS ++I+C
Sbjct: 687 EAPKFARELAGKIEVMENERVHFEARILPANDVKMTVEWYHNGNPLPAAHRFHPMFDFGYVALDLLYAYPQDSGTYTLVARNELGEARSNVELV-VGTEK-VLYLEPHHPEGLERIKELEQDRRQGI---AEVEDRTCDAAPKFLNDLPD-IQLNEHENIHVDLRATPVNDPTMVIEWFVNGRPLLTGSRVKTLNEFGFIALDIKGAIAEDSGTYSVRASNLLGEAIRQ-CVITVTPAGQILSDTQHQES-LGKINYLENLNK-YGRVEIEDKGPQEPPTFVVPLQADLGDVEEGEPIHLECQVNPINDNSLKIIWLRDGQSLPHGHRFRTFYDFGFVSLDILGFYAQDAGTYTCRAENSLGQAE---------TVATIRCAPKDAILGAVQHPRSYARIQEIEAPKPAPQE---VPDLPHQPPAFVKQLGPAIQCMEGDNVYLEAQVTPTDDNSLTYEWLVNGQPLMKAHRFVLSQDFGYIALNILYCYPEDNGTYTLVVRNRAGEAQS-TVDINC 1181 HSP 2 Score: 222.246 bits (565), Expect = 2.312e-59 Identity = 162/551 (29.40%), Postives = 269/551 (48.82%), Query Frame = 3
Query: 252 YDSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESG----VDYLTQLPEEMMQEINNLDKNYETGTYHGMEE-DKHAAKTIPKFLSQLPNSLTLIQ-GQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQ-SKGISAIHALEAELNAPKAEMKED-EIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADS---AGYQRLKDLEEDLASG 1871
+ PTF ++L + EG+ +RL R+ P +D ++ + W ++G+ L SRF + ++ LDIL +D+G Y C A S G A ++ + +G +D + + +QEI N +K +E D KT PKF++QL +SL +Q G P+H+E +E +D+ L +WF +G L G R +D GY ALDI + + +D G+++CVARNSLG+A+T N T P +I T S E S +++ LE P D P FIEP+D I E A+F+ + P L W+ +G+ + +R + +L+I +T+P D+G Y + G E + L V I+ D Q + I +E AP+A +E+ ++ P F+ QL +D + EG + P +D V+WY N N + SR + D G +L++ + ED+G+Y ++ N G+ ++ +SC T +++L D+ +QR++++E A G
Sbjct: 1484 FQPPTFTENLADKEDAQEGQSIRLECRLIPVNDPTMRVTWTRNGQPLPEASRFMPARNFDYVNLDILALYGEDSGVYTCKAVSAFGEAATSCT---VKCAAGKSLLLDTMHDASWKRVQEIENREK---------LEAVDAEPEKTAPKFVTQLNSSLGELQEGVPIHLEAQVEPTNDNQLTVQWFHNGQPLANGHRFRTRHDFGYVALDILYAFAQDTGEWACVARNSLGEAQT-IANFTVLPRGTIYTDSQHPE-SWQKIQVLEAPRAAAPEKPDAEH--DAPQFIEPLD--SIDRMEFQSAHFQTKVTPQTDPNLRIQWFKDGQPLMNSNRFKLTTDFGYISLDIAHTVPEDSGVYSVKASNAKGDAEVQ-AQLTVTGNAVILGDTQHEQSWQKIQLIE----APRAPGEEEPDVKHGPPKFVTQLHSLDGVVEGQPSHFEAQFIPFSDPKTSVQWYLNGNPLSASSRRILRNDFGLVSLDLQYTLGEDAGEYSVVVKNSEGEDRTSG-QLSC------TTRAAILGDTQHEQSWQRIQEIEAPRAPG 2004 HSP 3 Score: 219.935 bits (559), Expect = 1.246e-58 Identity = 164/575 (28.52%), Postives = 273/575 (47.48%), Query Frame = 3
Query: 168 TMAPHALEVVNQVNKRSLASPRRGSDHLYDSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTE---SSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPI-DPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKG--ISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGN 1874
++ P +L+ + Q+ A P D +++P L+ + ++EG L T+ P D S+++ W KDG+ + +R+ + D GF VLDIL+ ++ D G+Y ++SG A ++ + ++ SG+ L E+ + + L+ N +E K A P F+ L + +G H E +D+ L +W+ DG LK GSRI I GY L+I+ YPEDNG+Y C A N +G+A T S +TC P+E II+ + L E ++ ++ +E P AR + PP F + P E+ EG A+ + + P +L +W+HNG+ V +R+ + L + P D+G++ C ++HG E L V + + QS I LE ++ PK ++ + PSF + L + QLNE D+ +EP D +L V+W N + I +R+ + + G ATL I I+ D+G+Y +ATN G S G+ V +S+ + Q+L D E++ G+
Sbjct: 2645 SLQPQSLDRIRQIEAGKPA-PEERPDQQFEAPAIVNALQVQGALEEGGSAHLQTQFTPVADPSIKVEWLKDGQPIFHSNRYKMVHDFGFAVLDILHLLKHDAGEYTFRVSNNSGEASTSTS-FEVSESSGL-ILQPQNEQKAKAVEILEDNLRRRPEEIEQELKEAT---PVFIEPLSAPVETEEGGRAHFTARYEPVNDNQLQVQWYHDGRPLKNGSRIKTINSFGYVVLEISPTYPEDNGEYICRAVNRVGEAVT-STKLTCTPKEGIISATQLPERMANAGRRIAEIEAP--RPAREDAPDADHGPPKFTSALAGPPEL--QEGQQAHLECQVTPVADPRLVIEWFHNGQPVNHTNRMKAIHDFGFVVLQLTPAEPQDSGTWTCRATNQHGSDEVS-TELKVVGGGGVSYEWQSTAERKERITELEDWIHRPKEDLNLPAVDYPAPSFSQGLTDLGQLNEADATAFVCVLEPIGDPTLRVQWEHNGHPIPYSNRISCTNEFGVATLLIKHLIAADAGEYKCVATNVKGSATS--------VGKIAVESSTQIDAPQVVQQLVDSVENILEGD 3199 HSP 4 Score: 218.394 bits (555), Expect = 4.276e-58 Identity = 189/654 (28.90%), Postives = 299/654 (45.72%), Query Frame = 3
Query: 261 PTFQQDLESEL-VVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEM--MQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPN-SLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQL-EPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSA---GYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMN-ITAGEGGPAKFLVKVSGFPTPTL--EWRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLTV 2189
PT + L + + V E + L L +V P DD +L W +G L A SR+ + D GF+ LDI Y I +D+G Y V + +G A+++ +++ + T PE + ++E+ L D AA P F QL + L +GQ +H++ ++ +D SL EWF DG L GSRI I+D GY L+ +PED G Y+C A N +G+A T + C P +I ++ ESS +++ +EN +E R + PP+F E + +E EG + + P N + W NG+ + SR + L+IL D+G Y C S G + T+ KS++LD S E E N K E + E P F+ QL + +L EG + L +VEP NDN L V+W+ N + NG R + +D G L+I+ A ++D+G++ +A N G+ Q+ I+ F ++ ++ DS +Q+++ LE A+ D + + P F ++P++ I E A F KV+ P L +W D ++ + K D L+I V A+N G A+ +A LTV
Sbjct: 1221 PTIAKPLPATIDSVHESQTLHLEAQVTPIDDNTLRYEWLFNGNPLKASSRYRVLNDFGFVSLDIDYIIAEDSGKYTLVVYNSAGRAETS-CEFQVDRLKSILSDTAHPESLRRIREMEQLQP--------AKPSDDDAAAQPPVFTQQLTGPTEPLKEGQSVHMDCVVQPINDPSLRIEWFHDGRPLMFGSRIRTIHDFGYVGLEFLHIHPEDTGTYTCKATNLIGEA-TTDIFLECKPRRNIYLDTH-HESSWQKIQEIENRVDE--REPTPELTFQPPTFTENLADKE-DAQEGQSIRLECRLIPVNDPTMRVTWTRNGQPLPEASRFMPARNFDYVNLDILALYGEDSGVYTCKAVSAFGEAATS-CTVKCAAGKSLLLDTMHDA-SWKRVQEIE-NREKLEAVDAEPEKTAPKFVTQLNSSLGELQEGVPIHLEAQVEPTNDNQLTVQWFHNGQPLANGHRFRTRHDFGYVALDILYAFAQDTGEWACVARNSLGEAQT----IANF---TVLPRGTIYTDSQHPESWQKIQVLEAPRAAA-----PEKPDAEHDA-PQF-IEPLDSIDRMEFQSAHFQTKVTPQTDPNLRIQWFKDGQPLMNSNRFKLTTDFGYISLDIAHTVPEDSGVYSVKASNAKGDAEVQAQLTV 1843 HSP 5 Score: 197.978 bits (502), Expect = 1.302e-51 Identity = 175/657 (26.64%), Postives = 307/657 (46.73%), Query Frame = 3
Query: 15 NGAIFKCITRHKYSFIFNLDFIAMTTQESMEY-----NNNSNFNSVPDEGVIPNGT----TMAPHALEVVNQVNKRSLASPRRGSDHLYDSPTFQQDLESELVVD--EGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESII--TKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILD---PQS-KGISAIHALE--AELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNF 1928
NG F+ TRH + ++ LD + Q++ E+ N+ ++ + V+P GT + P + + + + A+P + D +D+P F + L+S +D E + T+V P D +L I WFKDG+ L +RF D G+I LDI +T+ +D+G Y A + G A+ A + + + + TQ E+ Q+I ++ G EE+ PKF++QL + +++GQP H E SD +W+ +G L SR + D G +LD+ + ED G+YS V +NS G+ T S ++C +I+ T+ + I ++++ PG E + PSF++P+ + EG+ A + + P N L W++N + + + ++ + +L I TG Y C +++ G + + VQ + ++LD P S + I + A++ A L+AP+ E ++ P +++ E + + EG +VTL VEP+ D L +EW N + N +R + Y+ G A L IV + DSG Y A N G+ S ++ EKI+ S ++R+++LE + E++ ++ PNF
Sbjct: 1665 NGHRFR--TRHDFGYV-ALDILYAFAQDTGEWACVARNSLGEAQTIANFTVLPRGTIYTDSQHPESWQKIQVLEAPRAAAPEK-PDAEHDAPQFIEPLDS---IDRMEFQSAHFQTKVTPQTDPNLRIQWFKDGQPLMNSNRFKLTTDFGYISLDIAHTVPEDSGVYSVKASNAKGDAEVQ-AQLTVTGNAVILGDTQH-EQSWQKIQLIEAPRAPG-----EEEPDVKHGPPKFVTQLHSLDGVVEGQPSHFEAQFIPFSDPKTSVQWYLNGNPLSASSRRILRNDFGLVSLDLQYTLGEDAGEYSVVVKNSEGEDRT-SGQLSCTTRAAILGDTQHEQSWQRIQEIEAPRAPGAE-----PEGPVYEKPSFVQPLQSVG-DLPEGSVALLEARLVPVNDPNLRVQWFYNDQPLMESNWISTSNDFGCVSLRIAPVYARHTGVYSCKAWNDSGNAVTS-ANVGVQGSEGLLLDTSHPASLQKIQELEAIDKYARLDAPEREYEK-------PQWVQGFENYENVGEGQTVTLHGLVEPSGDPHLRLEWLLNGTPLRNANRFRQEYEFGNAILTIVHVLPHDSGVYTCRAWNTQGEA-STSATVTTAGYEKILYDSQ---HPVSWERIQELEAP------KIVEEIEEIVQKEKPNF 2282 HSP 6 Score: 197.978 bits (502), Expect = 1.488e-51 Identity = 189/752 (25.13%), Postives = 321/752 (42.69%), Query Frame = 3
Query: 42 RHKYSFIF-NLDFIAMTTQESMEYNNNS-----------NFNSVPDEGVIPNGTTMAPHALEVVNQVNKRSLASPRRGSDHLYDSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRIN-PESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSL-TLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADS---AGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAG---EGGPAKFLVKVSGFPTP----TLEWRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLTVIPMVDFRPDLRH 2225
R Y F F +LD + Q++ Y + P + ++ G P + + ++ A P+ D + P F + L + EG+ + L +V P DD SL W +G+ L RF D G+I L+ILY +DNG Y V ++ +G AQS V IN +G ++ + I L+ + E K +P LP ++ ++ + Q LH+E + D++L EW +G LK SR V+ D G+ +LDI + ED+G Y+ V NS G+AET SC +SI++ + E S+ +++ +E + A+ +D PP F + + + EG + + P N L +W+H+G + SR+ + L L+ P DTG+Y C + G ++ + L + ++I LD + S+ ++ N E+ P+F + L + EG S+ L ++ P ND ++ V W +N + SR + + L+I++ EDSG Y A + G+ + + C G+ SLL D+ A ++R++++E N + + E+ P F V +N + G EG P +V PT T++W + + +T +D L+I + A N LG A+ A TV+P D +H
Sbjct: 1007 RTFYDFGFVSLDILGFYAQDAGTYTCRAENSLGQAETVATIRCAPKDAIL--GAVQHPRSYARIQEIEAPKPA-PQEVPDLPHQPPAFVKQLGPAIQCMEGDNVYLEAQVTPTDDNSLTYEWLVNGQPLMKAHRFVLSQDFGYIALNILYCYPEDNGTYTLVVRNRAGEAQST---VDINCGHTGGNFTDSFHPNSLHRIAELETPIQRA-----EPLPDKEKEVPTIAKPLPATIDSVHESQTLHLEAQVTPIDDNTLRYEWLFNGNPLKASSRYRVLNDFGFVSLDIDYIIAEDSGKYTLVVYNSAGRAET-SCEFQVDRLKSILSDTAHPE-SLRRIREMEQ--LQPAKPSDDDAAAQPPVFTQQLTGPTEPLKEGQSVHMDCVVQPINDPSLRIEWFHDGRPLMFGSRIRTIHDFGYVGLEFLHIHPEDTGTYTCKATNLIGEATTD-IFLECKPRRNIYLDTHHE--SSWQKIQEIENRVDEREPTPELTFQPPTFTENLADKEDAQEGQSIRLECRLIPVNDPTMRVTWTRNGQPLPEASRFMPARNFDYVNLDILALYGEDSGVYTCKAVSAFGEAAT-SCTVKCAAGK------SLLLDTMHDASWKRVQEIE------NREKLEAVDAEPEKTAPKF-VTQLNSSLGELQEGVPIHLEAQVE--PTNDNQLTVQWFHNGQPLANGHRFRTRHDFGYVALDILYAFAQDTGEWACVARNSLGEAQTIANFTVLPRGTIYTDSQH 1724 HSP 7 Score: 187.193 bits (474), Expect = 3.591e-48 Identity = 172/663 (25.94%), Postives = 282/663 (42.53%), Query Frame = 3
Query: 258 SPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGE-EYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAE---LNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEG-EKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNF--DVKPMNITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLTVIPMVDFRPDLRH 2225
SP F+ L+S+ +V EG+ RL + P +D +L I W +GK + A SR T D G L+I D G+Y VA + G A R++ V ++ Y++ G + +P F S L S + +GQ +H+E L +D L W DG L + G+ +LDI D+G YSC A N LG++E Q+ + I+ KS+L + + +E+ E ++A + + P F+ I P G ++F+ I P N L W +G+ + +R+ + +L++ T P D G Y CVLF+ HG+ +S V + + + + I L++ + E E+ + P F ++L ++ E + V ++ PAND + VEWY N N + R +D G L+++ A +DSG Y L+A N+ G+ +S N+ G EK++ + G +R+K+LE+D G ED + P F D+ + + E PT +EW ++ ++ S KT N+ L+I V A+N LG A + +TV P D +H
Sbjct: 297 SPKFEGSLQSQQLV-EGQSARLEIKYTPVEDPNLRIAWLLNGKGILASSRIVTFTDFGIAALEINPVNVFDQGEYTVVAVNPLGEA-------RVSANIAVIGHGSFIQQQQSGSQFGGTAYQS---KGAQAPAGTHLDLPNFHSDL-RSQEVFEGQQIHLETKLTPINDPDLRVVWLLDGNELPSNDKYRQTLSHGFASLDIPQTSSNDSGLYSCRAFNKLGESENQATII-------IVPKSDLQQFEQHRQLDVEDVREIQFAHSSQD----LTPKFLSQIQPFHCEQELGR-SFFEARIQPINDPTLRVSWLKDGQPLPNANRIQIFQNFGVVSLSLHPTYPEDAGVYTCVLFNSHGQAQSSAELTTVWIDTLQLDSKHADSLPIIGYLDSHQIHIGPQSVERPEEFNSLEAPKFARELAGKIEVMENERVHFEARILPANDVKMTVEWYHNGNPLPAAHRFHPMFDFGYVALDLLYAYPQDSGTYTLVARNELGEARS---NVELVVGTEKVLYLEPHHPE--GLERIKELEQDRRQG----IAEVEDRTCDAAPKFLNDLPDIQLNEHENIHVDLRATPVNDPTMVIEWFVNGRPLLTGSRVKTLNEFGFIALDIKGAIAEDSGTYSVRASNLLGEAIRQCVITVTPAGQILSDTQH 926 HSP 8 Score: 186.808 bits (473), Expect = 5.439e-48 Identity = 192/783 (24.52%), Postives = 329/783 (42.02%), Query Frame = 3
Query: 66 NLDFIAMTTQESMEYNNNSNFNSVPDEGVIPNGTTMAPHALEVVNQVNKRSLASPRR--GSDHLYDSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKT---IPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAEL---NAPKAEMKEDEIITD----LPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQ--EECLPNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLVVPDSVCKTYNDGSINYLEI--NRCSELGDIKVHVAATNPLGVAKEEATLTVIPMVDFRPDLRHITPENPFKKMAGLKKVECTSE------LNDAFKKS-KPTAERIVKIE 2345
N ++I E ++ P EG+I + T + ++ + P R D + P F L + EG+ L +V P D L I WF +G+ ++ +R I D GF+VL + D+G + C A + G+ + + +++ GV Y Q E + I L+ H +ED + P F L + L + LE D +L +W +G + +RI + G L I D G+Y CVA N G A +S+ ++ ++ I P + +D E ++ EG + + + P N +L +W NG + SR E +L+ILY P D G Y V+ ++ G S+ + V S+ Q+ G + +LE+ + K ++ ++I + P F QL+ I L EG+ +V P ND L VEW+K++ ++ G R + + D G A L+++ A+ +D+G+Y +ATN++GQT ++C ++T S + R+ ++++D + NI + VQ ++ P F + N+ E PA+F V+G+P P + W I+ + K DG ++YL + +R S+ G+ V A N G ATL + DFR + P N FK L++ E + L AF+ + KP A++++ +E
Sbjct: 2879 NGEYICRAVNRVGEAVTSTKLTCTPKEGII-SATQLPERMANAGRRIAEIEAPRPAREDAPDADHGPPKFTSALAGPPELQEGQQAHLECQVTPVADPRLVIEWFHNGQPVNHTNRMKAIHDFGFVVLQLTPAEPQDSGTWTCRATNQHGSDEVS-TELKVVGGGGVSYEWQSTAERKERITELED-----WIHRPKEDLNLPAVDYPAPSFSQGLTDLGQLNEADATAFVCVLEPIGDPTLRVQWEHNGHPIPYSNRISCTNEFGVATLLIKHLIAADAGEYKCVATNVKGSA-----------------------TSVGKIA-----------VESSTQIDAPQVVQQLVDSVE-NILEGDSIHLECRVTPINDPRLHVEWLRNGAPLPEASRFKPTFEFGFVSLDILYAYPEDNGDYELVVRNDKGEARSK-TKITVLPRPSLDYTSQTHG-NQQDSLESHFKQHSQAKLQLTANDIYNESDKRAPEFRTQLQNIGVL-EGEFCRFETQVAPINDPYLKVEWFKDKKPVLLGHRFRSTLDFGFACLDLLYALPDDTGEYHCVATNRHGQTMI-SAKLACQGASHVITDSQMPQGL----RVSNVKKD--NKNIYWSEQGGAVQPKQKQAPQFTIPLRNLQVTENQPARFECAVTGYPRPKVTWFINGNQCLHGHRFKLNFDG-LHYLTVSKSRISDAGE--VVAIARNTEGETISTATLDIFQNDDFR--QTKLRPAN-FKTSDELRERELQWQRDTLGSLGPAFEAAPKPDAQKLMHVE 3603 HSP 9 Score: 186.808 bits (473), Expect = 6.154e-48 Identity = 146/580 (25.17%), Postives = 259/580 (44.66%), Query Frame = 3
Query: 249 LYDSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGV--DYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQS-KGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAGEGGPAKF 1979
+YD P ++ + +EG+ + + P +D L++ W ++G L+ GS+++ D G LD+ YT +D G Y + G A S+ A ++ + + + D Q + +QEI + +EE K P+F+ QL + +L++ QP H E +E D +L WF +G + SR+ +I D G+ +DIA P D+G++ CVA+N+ G+A + + +E +I + +L S+ +++ +E D P+ + + + ++ EG A+ + P + +W +G+ + +R + + L+IL+ L D G Y + + G S + V +IL PQ+ + A+ LE L + E E E+ P FI+ L + EG T + EP NDN L V+WY + + NGSR++ G L I ED+G+Y A N+ G+ + ++C E I++A+ L A R I+ + +E+ P+ D P T+ GP +
Sbjct: 2408 VYDHPAITTQIDDK-ECNEGDHVHFEALITPVNDPRLQVQWIRNGVPLAHGSKYAIQQDFGICTLDVAYTYPEDEGVYQLRIWNPEGEAVSS-ATLKCHGKDAILGDVQHQESWKRIQEIEAPKQK--------LEEADPEPKGPPRFIQQLTSPGSLVENQPAHFEATVEPVDDPTLTINWFLNGEPMSASSRVKMINDFGWVIMDIAQTEPRDSGEWKCVAKNAAGEA-VSTATIEVQGKE-VILQDSLQPQSLDRIRQIEAGKPAPEERPDQQF--EAPAIVNALQ-VQGALEEGGSAHLQTQFTPVADPSIKVEWLKDGQPIFHSNRYKMVHDFGFAVLDILHLLKHDAGEYTFRVSNNSGEA-STSTSFEVSESSGLILQPQNEQKAKAVEILEDNLRR-RPEEIEQELKEATPVFIEPLSAPVETEEGGRAHFTARYEPVNDNQLQVQWYHDGRPLKNGSRIKTINSFGYVVLEISPTYPEDNGEYICRAVNRVGEAVT-STKLTCTPKEGIISATQLPERMANAGR-----------RIAEIEAPRPAREDA-PDADHGPPKFTSALAGPPEL 2957 HSP 10 Score: 154.451 bits (389), Expect = 9.259e-38 Identity = 140/509 (27.50%), Postives = 223/509 (43.81%), Query Frame = 3
Query: 243 DHLYDSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALE----AELNAPKAEMKEDEIIT-DLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQS 1754
D + P F L S V EG+P + P D + W+ +G LSA SR D G + LD+ YT+ +D G+Y V K+ G +++ + + + TQ E+ Q I ++ G E + P F+ L + L +G +E L +D +L +WF + L + I D G +L IA Y G YSC A N G A T S NV E ++ ++ +S+ +++ LE ++YAR + P +++ + E +V EG ++P L +W NG ++ +R + E + L I++ LP D+G Y C ++ G + EK I+ D Q H + EL APK + +EI+ + P+F+ QLE + + EG + L +PA D L V W KN + +Q ++ G ATL+I SA + +G Y L TN G+ S
Sbjct: 1877 DVKHGPPKFVTQLHSLDGVVEGQPSHFEAQFIPFSDPKTSVQWYLNGNPLSASSRRILRNDFGLVSLDLQYTLGEDAGEYSVVVKNSEGEDRTS-GQLSCTTRAAILGDTQH-EQSWQRIQEIEAPRAPGA-----EPEGPVYEKPSFVQPLQSVGDLPEGSVALLEARLVPVNDPNLRVQWFYNDQPLMESNWISTSNDFGCVSLRIAPVYARHTGVYSCKAWNDSGNAVT-SANVGVQGSEGLLLDTS-HPASLQKIQELEAI-DKYARLDAPEREYEKPQWVQGFENYE-NVGEGQTVTLHGLVEPSGDPHLRLEWLLNGTPLRNANRFRQEYEFGNAILTIVHVLPHDSGVYTCRAWNTQGEASTSATVTTAGYEK-ILYDSQ-------HPVSWERIQELEAPKIVEEIEEIVQKEKPNFLTQLESAENVPEGVPLHLESTFQPARDPELKVVWQKNGQPLGASQLVQTKHELGWATLDISSANEDHNGVYTLTITNSEGEAVS 2366 HSP 11 Score: 118.627 bits (296), Expect = 1.112e-26 Identity = 127/506 (25.10%), Postives = 211/506 (41.70%), Query Frame = 3
Query: 255 DSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQS-CNVTCHPEESIITKSNLTESSIS-QLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEII---TDL-PSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQS 1754
++P F +S + D G+ ++L + A +G + WFKD + +S+G ++ L I + G Y C A + GT R+ S + EM+ + +DK + T +D A+K+ PKF L S L++GQ +EI D +L W +G + SRI D G AL+I D G+Y+ VA N LG+A + V H +S + Q K + P + P+F D R V EG + + + P N L W +G + + + L ++L+I T +D+G Y C F++ G E+ + +II+ P+S + E +++E + DL P F+ Q++P E +++P ND +L V W K+ + N +R+QI + G +L++ ED+G Y + N +GQ QS
Sbjct: 165 EAPEFTGKFQSTTLYD-GDSVKLYCKA--AGEG-VSFKWFKDNEPISSGGSYAVDNKGNETTLHINNATMKEGGWYRCDATNKHGT---TTLKGRVVVNSRQKFNGPAHREMIT-LRKVDKVERSRTPVNQLQDVSASKSSPKFEGSL-QSQQLVEGQSARLEIKYTPVEDPNLRIAWLLNGKGILASSRIVTFTDFGIAALEINPVNVFDQGEYTVVAVNPLGEARVSANIAVIGHGSFIQQQQSGSQFGGTAYQSKGAQAPAGTHLDL---------PNFHS--DLRSQEVFEGQQIHLETKLTPINDPDLRVVWLLDGNELPSNDKYRQTLSHGFASLDIPQTSSNDSGLYSCRAFNKLGESEN---------QATIIIVPKSD----LQQFEQHRQLDVEDVREIQFAHSSQDLTPKFLSQIQPFHCEQELGRSFFEARIQPINDPTLRVSWLKDGQPLPNANRIQIFQNFGVVSLSLHPTYPEDAGVYTCVLFNSHGQAQS 637 HSP 12 Score: 58.151 bits (139), Expect = 3.066e-8 Identity = 57/224 (25.45%), Postives = 93/224 (41.52%), Query Frame = 3
Query: 1530 LNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQ-EECLPNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLE--WRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLTVI 2192
L +GDSV L K A + +W+K+ I +G + TL+I +A ++ G Y ATNK+G T +G +V + A + + + D + + +DV + P F+ + EG A+ +K + P L W ++ ++ S T+ D I LEIN + + V A NPLG A+ A + VI
Sbjct: 178 LYDGDSVKLYCK---AAGEGVSFKWFKDNEPISSGGSYAVDNKGNETTLHINNATMKEGGWYRCDATNKHGTT--------TLKGRVVVNSRQKFNGPAHREMITLRKVDKVERSRTPVNQLQDVSASKSSPKFEGSLQSQQLVEGQSARLEIKYTPVEDPNLRIAWLLNGKGILASSRIVTFTDFGIAALEINPVNVFDQGEYTVVAVNPLGEARVSANIAVI 390 HSP 13 Score: 51.9878 bits (123), Expect = 2.720e-6 Identity = 78/364 (21.43%), Postives = 131/364 (35.99%), Query Frame = 3
Query: 666 AKTIPKFLSQLPNSLTLIQGQPLHIEIF-LEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQS 1754
+K P +SQ L +QG+ F +E + + W KDG +K R + +L I G+Y+ N+ G E+ + N+T P + + + AR ND + + + PA F I G K + W+ +G+ + R E L + +D G Y V + G + LNV +K+ KG P+ E P F Q++PI ++EG + + D + + WY+N + + +IS +G A L I +A +ED +Y + A+N G+ S
Sbjct: 3828 SKQAPPTISQ---QLKPLQGELGKAAKFVIEFAGAAPVKVTWLKDGKEIKSTFRTLITTTPTNSSLHIGRLENSHAGEYTVRLENAAGTVESLA-NLTVAPPTTQGKAPDFS-----------------ARLND------------------LRIQQNGPAEFSCQI--GGEPKPTIQWFKDGQPLPNDDRFQVVEEGGAYKLKFSNIISTDAGIYEIVAKNGVGEARCK-ARLNVNLQKT------GKGAE---------EGPRYEA---------PRFTSQIQPI-VVDEGKGAQFSAQFSGFPDPT--IRWYRNNEPVKHADGYEISQSKGEAILRISAARNEDVAEYKVEASNPAGKASS 4122
BLAST of Titin vs. Ensembl Celegans
Match: ketn-1 (KETtiN (Drosophila actin-binding) homolog; Kettin [Source:UniProtKB/TrEMBL;Acc:Q9NL87]) HSP 1 Score: 237.269 bits (604), Expect = 2.415e-64 Identity = 162/517 (31.33%), Postives = 258/517 (49.90%), Query Frame = 3
Query: 255 DSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPM-PPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAI-----HALEAELNAPKAEMKEDEIITDLP----SFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISC 1775
++P F ++L ++ V E E + R+ PA+D + + W+ +G L A RF +FD G++ LD+LY D+G Y VA++ G A+SN+ V + E V YL E ++ I L+++ G E + PKFL+ LP+ + L + + +H+++ +D ++V EWF +G L GSR+ + + G+ ALDI ED+G YS A N LG+A Q C +T P I++ + ES + ++ LEN + Y R P PP+F+ P+ V EG P + + ++P N L W +G+ + R + +L+IL D G+Y C + G+ E T +I P+ + A+ +A E+ APK +E + DLP +F+KQL P Q EGD+V L +V P +DNSL EW N ++ R +S D G LNI+ ED+G Y L+ N+ G+ QS ++I+C
Sbjct: 1326 EAPKFARELAGKIEVMENERVHFEARILPANDVKMTVEWYHNGNPLPAAHRFHPMFDFGYVALDLLYAYPQDSGTYTLVARNELGEARSNVELV-VGTEK-VLYLEPHHPEGLERIKELEQDRRQGI---AEVEDRTCDAAPKFLNDLPD-IQLNEHENIHVDLRATPVNDPTMVIEWFVNGRPLLTGSRVKTLNEFGFIALDIKGAIAEDSGTYSVRASNLLGEAIRQ-CVITVTPAGQILSDTQHQES-LGKINYLENLNK-YGRVEIEDKGPQEPPTFVVPLQADLGDVEEGEPIHLECQVNPINDNSLKIIWLRDGQSLPHGHRFRTFYDFGFVSLDILGFYAQDAGTYTCRAENSLGQAE---------TVATIRCAPKDAILGAVQHPRSYARIQEIEAPKPAPQE---VPDLPHQPPAFVKQLGPAIQCMEGDNVYLEAQVTPTDDNSLTYEWLVNGQPLMKAHRFVLSQDFGYIALNILYCYPEDNGTYTLVVRNRAGEAQS-TVDINC 1820 HSP 2 Score: 222.246 bits (565), Expect = 2.241e-59 Identity = 162/551 (29.40%), Postives = 269/551 (48.82%), Query Frame = 3
Query: 252 YDSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESG----VDYLTQLPEEMMQEINNLDKNYETGTYHGMEE-DKHAAKTIPKFLSQLPNSLTLIQ-GQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQ-SKGISAIHALEAELNAPKAEMKED-EIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADS---AGYQRLKDLEEDLASG 1871
+ PTF ++L + EG+ +RL R+ P +D ++ + W ++G+ L SRF + ++ LDIL +D+G Y C A S G A ++ + +G +D + + +QEI N +K +E D KT PKF++QL +SL +Q G P+H+E +E +D+ L +WF +G L G R +D GY ALDI + + +D G+++CVARNSLG+A+T N T P +I T S E S +++ LE P D P FIEP+D I E A+F+ + P L W+ +G+ + +R + +L+I +T+P D+G Y + G E + L V I+ D Q + I +E AP+A +E+ ++ P F+ QL +D + EG + P +D V+WY N N + SR + D G +L++ + ED+G+Y ++ N G+ ++ +SC T +++L D+ +QR++++E A G
Sbjct: 2123 FQPPTFTENLADKEDAQEGQSIRLECRLIPVNDPTMRVTWTRNGQPLPEASRFMPARNFDYVNLDILALYGEDSGVYTCKAVSAFGEAATSCT---VKCAAGKSLLLDTMHDASWKRVQEIENREK---------LEAVDAEPEKTAPKFVTQLNSSLGELQEGVPIHLEAQVEPTNDNQLTVQWFHNGQPLANGHRFRTRHDFGYVALDILYAFAQDTGEWACVARNSLGEAQT-IANFTVLPRGTIYTDSQHPE-SWQKIQVLEAPRAAAPEKPDAEH--DAPQFIEPLD--SIDRMEFQSAHFQTKVTPQTDPNLRIQWFKDGQPLMNSNRFKLTTDFGYISLDIAHTVPEDSGVYSVKASNAKGDAEVQ-AQLTVTGNAVILGDTQHEQSWQKIQLIE----APRAPGEEEPDVKHGPPKFVTQLHSLDGVVEGQPSHFEAQFIPFSDPKTSVQWYLNGNPLSASSRRILRNDFGLVSLDLQYTLGEDAGEYSVVVKNSEGEDRTSG-QLSC------TTRAAILGDTQHEQSWQRIQEIEAPRAPG 2643 HSP 3 Score: 219.55 bits (558), Expect = 1.540e-58 Identity = 164/575 (28.52%), Postives = 273/575 (47.48%), Query Frame = 3
Query: 168 TMAPHALEVVNQVNKRSLASPRRGSDHLYDSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTE---SSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPI-DPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKG--ISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGN 1874
++ P +L+ + Q+ A P D +++P L+ + ++EG L T+ P D S+++ W KDG+ + +R+ + D GF VLDIL+ ++ D G+Y ++SG A ++ + ++ SG+ L E+ + + L+ N +E K A P F+ L + +G H E +D+ L +W+ DG LK GSRI I GY L+I+ YPEDNG+Y C A N +G+A T S +TC P+E II+ + L E ++ ++ +E P AR + PP F + P E+ EG A+ + + P +L +W+HNG+ V +R+ + L + P D+G++ C ++HG E L V + + QS I LE ++ PK ++ + PSF + L + QLNE D+ +EP D +L V+W N + I +R+ + + G ATL I I+ D+G+Y +ATN G S G+ V +S+ + Q+L D E++ G+
Sbjct: 3284 SLQPQSLDRIRQIEAGKPA-PEERPDQQFEAPAIVNALQVQGALEEGGSAHLQTQFTPVADPSIKVEWLKDGQPIFHSNRYKMVHDFGFAVLDILHLLKHDAGEYTFRVSNNSGEASTSTS-FEVSESSGL-ILQPQNEQKAKAVEILEDNLRRRPEEIEQELKEAT---PVFIEPLSAPVETEEGGRAHFTARYEPVNDNQLQVQWYHDGRPLKNGSRIKTINSFGYVVLEISPTYPEDNGEYICRAVNRVGEAVT-STKLTCTPKEGIISATQLPERMANAGRRIAEIEAP--RPAREDAPDADHGPPKFTSALAGPPEL--QEGQQAHLECQVTPVADPRLVIEWFHNGQPVNHTNRMKAIHDFGFVVLQLTPAEPQDSGTWTCRATNQHGSDEVS-TELKVVGGGGVSYEWQSTAERKERITELEDWIHRPKEDLNLPAVDYPAPSFSQGLTDLGQLNEADATAFVCVLEPIGDPTLRVQWEHNGHPIPYSNRISCTNEFGVATLLIKHLIAADAGEYKCVATNVKGSATS--------VGKIAVESSTQIDAPQVVQQLVDSVENILEGD 3838 HSP 4 Score: 218.009 bits (554), Expect = 4.618e-58 Identity = 189/654 (28.90%), Postives = 299/654 (45.72%), Query Frame = 3
Query: 261 PTFQQDLESEL-VVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEM--MQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPN-SLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQL-EPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSA---GYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMN-ITAGEGGPAKFLVKVSGFPTPTL--EWRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLTV 2189
PT + L + + V E + L L +V P DD +L W +G L A SR+ + D GF+ LDI Y I +D+G Y V + +G A+++ +++ + T PE + ++E+ L D AA P F QL + L +GQ +H++ ++ +D SL EWF DG L GSRI I+D GY L+ +PED G Y+C A N +G+A T + C P +I ++ ESS +++ +EN +E R + PP+F E + +E EG + + P N + W NG+ + SR + L+IL D+G Y C S G + T+ KS++LD S E E N K E + E P F+ QL + +L EG + L +VEP NDN L V+W+ N + NG R + +D G L+I+ A ++D+G++ +A N G+ Q+ I+ F ++ ++ DS +Q+++ LE A+ D + + P F ++P++ I E A F KV+ P L +W D ++ + K D L+I V A+N G A+ +A LTV
Sbjct: 1860 PTIAKPLPATIDSVHESQTLHLEAQVTPIDDNTLRYEWLFNGNPLKASSRYRVLNDFGFVSLDIDYIIAEDSGKYTLVVYNSAGRAETS-CEFQVDRLKSILSDTAHPESLRRIREMEQLQP--------AKPSDDDAAAQPPVFTQQLTGPTEPLKEGQSVHMDCVVQPINDPSLRIEWFHDGRPLMFGSRIRTIHDFGYVGLEFLHIHPEDTGTYTCKATNLIGEA-TTDIFLECKPRRNIYLDTH-HESSWQKIQEIENRVDE--REPTPELTFQPPTFTENLADKE-DAQEGQSIRLECRLIPVNDPTMRVTWTRNGQPLPEASRFMPARNFDYVNLDILALYGEDSGVYTCKAVSAFGEAATS-CTVKCAAGKSLLLDTMHDA-SWKRVQEIE-NREKLEAVDAEPEKTAPKFVTQLNSSLGELQEGVPIHLEAQVEPTNDNQLTVQWFHNGQPLANGHRFRTRHDFGYVALDILYAFAQDTGEWACVARNSLGEAQT----IANF---TVLPRGTIYTDSQHPESWQKIQVLEAPRAAA-----PEKPDAEHDA-PQF-IEPLDSIDRMEFQSAHFQTKVTPQTDPNLRIQWFKDGQPLMNSNRFKLTTDFGYISLDIAHTVPEDSGVYSVKASNAKGDAEVQAQLTV 2482 HSP 5 Score: 197.978 bits (502), Expect = 1.416e-51 Identity = 175/657 (26.64%), Postives = 307/657 (46.73%), Query Frame = 3
Query: 15 NGAIFKCITRHKYSFIFNLDFIAMTTQESMEY-----NNNSNFNSVPDEGVIPNGT----TMAPHALEVVNQVNKRSLASPRRGSDHLYDSPTFQQDLESELVVD--EGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESII--TKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILD---PQS-KGISAIHALE--AELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNF 1928
NG F+ TRH + ++ LD + Q++ E+ N+ ++ + V+P GT + P + + + + A+P + D +D+P F + L+S +D E + T+V P D +L I WFKDG+ L +RF D G+I LDI +T+ +D+G Y A + G A+ A + + + + TQ E+ Q+I ++ G EE+ PKF++QL + +++GQP H E SD +W+ +G L SR + D G +LD+ + ED G+YS V +NS G+ T S ++C +I+ T+ + I ++++ PG E + PSF++P+ + EG+ A + + P N L W++N + + + ++ + +L I TG Y C +++ G + + VQ + ++LD P S + I + A++ A L+AP+ E ++ P +++ E + + EG +VTL VEP+ D L +EW N + N +R + Y+ G A L IV + DSG Y A N G+ S ++ EKI+ S ++R+++LE + E++ ++ PNF
Sbjct: 2304 NGHRFR--TRHDFGYV-ALDILYAFAQDTGEWACVARNSLGEAQTIANFTVLPRGTIYTDSQHPESWQKIQVLEAPRAAAPEK-PDAEHDAPQFIEPLDS---IDRMEFQSAHFQTKVTPQTDPNLRIQWFKDGQPLMNSNRFKLTTDFGYISLDIAHTVPEDSGVYSVKASNAKGDAEVQ-AQLTVTGNAVILGDTQH-EQSWQKIQLIEAPRAPG-----EEEPDVKHGPPKFVTQLHSLDGVVEGQPSHFEAQFIPFSDPKTSVQWYLNGNPLSASSRRILRNDFGLVSLDLQYTLGEDAGEYSVVVKNSEGEDRT-SGQLSCTTRAAILGDTQHEQSWQRIQEIEAPRAPGAE-----PEGPVYEKPSFVQPLQSVG-DLPEGSVALLEARLVPVNDPNLRVQWFYNDQPLMESNWISTSNDFGCVSLRIAPVYARHTGVYSCKAWNDSGNAVTS-ANVGVQGSEGLLLDTSHPASLQKIQELEAIDKYARLDAPEREYEK-------PQWVQGFENYENVGEGQTVTLHGLVEPSGDPHLRLEWLLNGTPLRNANRFRQEYEFGNAILTIVHVLPHDSGVYTCRAWNTQGEA-STSATVTTAGYEKILYDSQ---HPVSWERIQELEAP------KIVEEIEEIVQKEKPNF 2921 HSP 6 Score: 197.593 bits (501), Expect = 1.832e-51 Identity = 189/752 (25.13%), Postives = 321/752 (42.69%), Query Frame = 3
Query: 42 RHKYSFIF-NLDFIAMTTQESMEYNNNS-----------NFNSVPDEGVIPNGTTMAPHALEVVNQVNKRSLASPRRGSDHLYDSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRIN-PESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSL-TLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADS---AGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAG---EGGPAKFLVKVSGFPTP----TLEWRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLTVIPMVDFRPDLRH 2225
R Y F F +LD + Q++ Y + P + ++ G P + + ++ A P+ D + P F + L + EG+ + L +V P DD SL W +G+ L RF D G+I L+ILY +DNG Y V ++ +G AQS V IN +G ++ + I L+ + E K +P LP ++ ++ + Q LH+E + D++L EW +G LK SR V+ D G+ +LDI + ED+G Y+ V NS G+AET SC +SI++ + E S+ +++ +E + A+ +D PP F + + + EG + + P N L +W+H+G + SR+ + L L+ P DTG+Y C + G ++ + L + ++I LD + S+ ++ N E+ P+F + L + EG S+ L ++ P ND ++ V W +N + SR + + L+I++ EDSG Y A + G+ + + C G+ SLL D+ A ++R++++E N + + E+ P F V +N + G EG P +V PT T++W + + +T +D L+I + A N LG A+ A TV+P D +H
Sbjct: 1646 RTFYDFGFVSLDILGFYAQDAGTYTCRAENSLGQAETVATIRCAPKDAIL--GAVQHPRSYARIQEIEAPKPA-PQEVPDLPHQPPAFVKQLGPAIQCMEGDNVYLEAQVTPTDDNSLTYEWLVNGQPLMKAHRFVLSQDFGYIALNILYCYPEDNGTYTLVVRNRAGEAQST---VDINCGHTGGNFTDSFHPNSLHRIAELETPIQRA-----EPLPDKEKEVPTIAKPLPATIDSVHESQTLHLEAQVTPIDDNTLRYEWLFNGNPLKASSRYRVLNDFGFVSLDIDYIIAEDSGKYTLVVYNSAGRAET-SCEFQVDRLKSILSDTAHPE-SLRRIREMEQ--LQPAKPSDDDAAAQPPVFTQQLTGPTEPLKEGQSVHMDCVVQPINDPSLRIEWFHDGRPLMFGSRIRTIHDFGYVGLEFLHIHPEDTGTYTCKATNLIGEATTD-IFLECKPRRNIYLDTHHE--SSWQKIQEIENRVDEREPTPELTFQPPTFTENLADKEDAQEGQSIRLECRLIPVNDPTMRVTWTRNGQPLPEASRFMPARNFDYVNLDILALYGEDSGVYTCKAVSAFGEAAT-SCTVKCAAGK------SLLLDTMHDASWKRVQEIE------NREKLEAVDAEPEKTAPKF-VTQLNSSLGELQEGVPIHLEAQVE--PTNDNQLTVQWFHNGQPLANGHRFRTRHDFGYVALDILYAFAQDTGEWACVARNSLGEAQTIANFTVLPRGTIYTDSQH 2363 HSP 7 Score: 187.193 bits (474), Expect = 3.971e-48 Identity = 172/663 (25.94%), Postives = 282/663 (42.53%), Query Frame = 3
Query: 258 SPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGE-EYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAE---LNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEG-EKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNF--DVKPMNITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLTVIPMVDFRPDLRH 2225
SP F+ L+S+ +V EG+ RL + P +D +L I W +GK + A SR T D G L+I D G+Y VA + G A R++ V ++ Y++ G + +P F S L S + +GQ +H+E L +D L W DG L + G+ +LDI D+G YSC A N LG++E Q+ + I+ KS+L + + +E+ E ++A + + P F+ I P G ++F+ I P N L W +G+ + +R+ + +L++ T P D G Y CVLF+ HG+ +S V + + + + I L++ + E E+ + P F ++L ++ E + V ++ PAND + VEWY N N + R +D G L+++ A +DSG Y L+A N+ G+ +S N+ G EK++ + G +R+K+LE+D G ED + P F D+ + + E PT +EW ++ ++ S KT N+ L+I V A+N LG A + +TV P D +H
Sbjct: 936 SPKFEGSLQSQQLV-EGQSARLEIKYTPVEDPNLRIAWLLNGKGILASSRIVTFTDFGIAALEINPVNVFDQGEYTVVAVNPLGEA-------RVSANIAVIGHGSFIQQQQSGSQFGGTAYQS---KGAQAPAGTHLDLPNFHSDL-RSQEVFEGQQIHLETKLTPINDPDLRVVWLLDGNELPSNDKYRQTLSHGFASLDIPQTSSNDSGLYSCRAFNKLGESENQATII-------IVPKSDLQQFEQHRQLDVEDVREIQFAHSSQD----LTPKFLSQIQPFHCEQELGR-SFFEARIQPINDPTLRVSWLKDGQPLPNANRIQIFQNFGVVSLSLHPTYPEDAGVYTCVLFNSHGQAQSSAELTTVWIDTLQLDSKHADSLPIIGYLDSHQIHIGPQSVERPEEFNSLEAPKFARELAGKIEVMENERVHFEARILPANDVKMTVEWYHNGNPLPAAHRFHPMFDFGYVALDLLYAYPQDSGTYTLVARNELGEARS---NVELVVGTEKVLYLEPHHPE--GLERIKELEQDRRQG----IAEVEDRTCDAAPKFLNDLPDIQLNEHENIHVDLRATPVNDPTMVIEWFVNGRPLLTGSRVKTLNEFGFIALDIKGAIAEDSGTYSVRASNLLGEAIRQCVITVTPAGQILSDTQH 1565 HSP 8 Score: 186.808 bits (473), Expect = 5.604e-48 Identity = 146/580 (25.17%), Postives = 259/580 (44.66%), Query Frame = 3
Query: 249 LYDSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGV--DYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQS-KGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAGEGGPAKF 1979
+YD P ++ + +EG+ + + P +D L++ W ++G L+ GS+++ D G LD+ YT +D G Y + G A S+ A ++ + + + D Q + +QEI + +EE K P+F+ QL + +L++ QP H E +E D +L WF +G + SR+ +I D G+ +DIA P D+G++ CVA+N+ G+A + + +E +I + +L S+ +++ +E D P+ + + + ++ EG A+ + P + +W +G+ + +R + + L+IL+ L D G Y + + G S + V +IL PQ+ + A+ LE L + E E E+ P FI+ L + EG T + EP NDN L V+WY + + NGSR++ G L I ED+G+Y A N+ G+ + ++C E I++A+ L A R I+ + +E+ P+ D P T+ GP +
Sbjct: 3047 VYDHPAITTQIDDK-ECNEGDHVHFEALITPVNDPRLQVQWIRNGVPLAHGSKYAIQQDFGICTLDVAYTYPEDEGVYQLRIWNPEGEAVSS-ATLKCHGKDAILGDVQHQESWKRIQEIEAPKQK--------LEEADPEPKGPPRFIQQLTSPGSLVENQPAHFEATVEPVDDPTLTINWFLNGEPMSASSRVKMINDFGWVIMDIAQTEPRDSGEWKCVAKNAAGEA-VSTATIEVQGKE-VILQDSLQPQSLDRIRQIEAGKPAPEERPDQQF--EAPAIVNALQ-VQGALEEGGSAHLQTQFTPVADPSIKVEWLKDGQPIFHSNRYKMVHDFGFAVLDILHLLKHDAGEYTFRVSNNSGEA-STSTSFEVSESSGLILQPQNEQKAKAVEILEDNLRR-RPEEIEQELKEATPVFIEPLSAPVETEEGGRAHFTARYEPVNDNQLQVQWYHDGRPLKNGSRIKTINSFGYVVLEISPTYPEDNGEYICRAVNRVGEAVT-STKLTCTPKEGIISATQLPERMANAGR-----------RIAEIEAPRPAREDA-PDADHGPPKFTSALAGPPEL 3596 HSP 9 Score: 186.422 bits (472), Expect = 6.511e-48 Identity = 197/808 (24.38%), Postives = 336/808 (41.58%), Query Frame = 3
Query: 15 NGAIFKCITRHKYSFIF--------NLDFIAMTTQESMEYNNNSNFNSVPDEGVIPNGTTMAPHALEVVNQVNKRSLASPRR--GSDHLYDSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKT---IPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAEL---NAPKAEMKEDEIITD----LPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQ--EECLPNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLVVPDSVCKTYNDGSINYLEI--NRCSELGDIKVHVAATNPLGVAKEEATLTVIPMVDFRPDLRHITPENPFKKMAGLKKVECTSE------LNDAFKKS-KPTAERIVKIE 2345
NG+ K I Y + N ++I E ++ P EG+I + T + ++ + P R D + P F L + EG+ L +V P D L I WF +G+ ++ +R I D GF+VL + D+G + C A + G+ + + +++ GV Y Q E + I L+ H +ED + P F L + L + LE D +L +W +G + +RI + G L I D G+Y CVA N G A +S+ ++ ++ I P + +D E ++ EG + + + P N +L +W NG + SR E +L+ILY P D G Y V+ ++ G S+ + V S+ Q+ G + +LE+ + K ++ ++I + P F QL+ I L EG+ +V P ND L VEW+K++ ++ G R + + D G A L+++ A+ +D+G+Y +ATN++GQT ++C ++T S + R+ ++++D + NI + VQ ++ P F + N+ E PA+F V+G+P P + W I+ + K DG ++YL + +R S+ G+ V A N G ATL + DFR + P N FK L++ E + L AF+ + KP A++++ +E
Sbjct: 3493 NGSRIKTINSFGYVVLEISPTYPEDNGEYICRAVNRVGEAVTSTKLTCTPKEGII-SATQLPERMANAGRRIAEIEAPRPAREDAPDADHGPPKFTSALAGPPELQEGQQAHLECQVTPVADPRLVIEWFHNGQPVNHTNRMKAIHDFGFVVLQLTPAEPQDSGTWTCRATNQHGSDEVS-TELKVVGGGGVSYEWQSTAERKERITELED-----WIHRPKEDLNLPAVDYPAPSFSQGLTDLGQLNEADATAFVCVLEPIGDPTLRVQWEHNGHPIPYSNRISCTNEFGVATLLIKHLIAADAGEYKCVATNVKGSA-----------------------TSVGKIA-----------VESSTQIDAPQVVQQLVDSVE-NILEGDSIHLECRVTPINDPRLHVEWLRNGAPLPEASRFKPTFEFGFVSLDILYAYPEDNGDYELVVRNDKGEARSK-TKITVLPRPSLDYTSQTHG-NQQDSLESHFKQHSQAKLQLTANDIYNESDKRAPEFRTQLQNIGVL-EGEFCRFETQVAPINDPYLKVEWFKDKKPVLLGHRFRSTLDFGFACLDLLYALPDDTGEYHCVATNRHGQTMI-SAKLACQGASHVITDSQMPQGL----RVSNVKKD--NKNIYWSEQGGAVQPKQKQAPQFTIPLRNLQVTENQPARFECAVTGYPRPKVTWFINGNQCLHGHRFKLNFDG-LHYLTVSKSRISDAGE--VVAIARNTEGETISTATLDIFQNDDFR--QTKLRPAN-FKTSDELRERELQWQRDTLGSLGPAFEAAPKPDAQKLMHVE 4242 HSP 10 Score: 154.066 bits (388), Expect = 1.036e-37 Identity = 140/509 (27.50%), Postives = 223/509 (43.81%), Query Frame = 3
Query: 243 DHLYDSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALE----AELNAPKAEMKEDEIIT-DLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQS 1754
D + P F L S V EG+P + P D + W+ +G LSA SR D G + LD+ YT+ +D G+Y V K+ G +++ + + + TQ E+ Q I ++ G E + P F+ L + L +G +E L +D +L +WF + L + I D G +L IA Y G YSC A N G A T S NV E ++ ++ +S+ +++ LE ++YAR + P +++ + E +V EG ++P L +W NG ++ +R + E + L I++ LP D+G Y C ++ G + EK I+ D Q H + EL APK + +EI+ + P+F+ QLE + + EG + L +PA D L V W KN + +Q ++ G ATL+I SA + +G Y L TN G+ S
Sbjct: 2516 DVKHGPPKFVTQLHSLDGVVEGQPSHFEAQFIPFSDPKTSVQWYLNGNPLSASSRRILRNDFGLVSLDLQYTLGEDAGEYSVVVKNSEGEDRTS-GQLSCTTRAAILGDTQH-EQSWQRIQEIEAPRAPGA-----EPEGPVYEKPSFVQPLQSVGDLPEGSVALLEARLVPVNDPNLRVQWFYNDQPLMESNWISTSNDFGCVSLRIAPVYARHTGVYSCKAWNDSGNAVT-SANVGVQGSEGLLLDTS-HPASLQKIQELEAI-DKYARLDAPEREYEKPQWVQGFENYE-NVGEGQTVTLHGLVEPSGDPHLRLEWLLNGTPLRNANRFRQEYEFGNAILTIVHVLPHDSGVYTCRAWNTQGEASTSATVTTAGYEK-ILYDSQ-------HPVSWERIQELEAPKIVEEIEEIVQKEKPNFLTQLESAENVPEGVPLHLESTFQPARDPELKVVWQKNGQPLGASQLVQTKHELGWATLDISSANEDHNGVYTLTITNSEGEAVS 3005 HSP 11 Score: 118.242 bits (295), Expect = 1.278e-26 Identity = 127/506 (25.10%), Postives = 211/506 (41.70%), Query Frame = 3
Query: 255 DSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQS-CNVTCHPEESIITKSNLTESSIS-QLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEII---TDL-PSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQS 1754
++P F +S + D G+ ++L + A +G + WFKD + +S+G ++ L I + G Y C A + GT R+ S + EM+ + +DK + T +D A+K+ PKF L S L++GQ +EI D +L W +G + SRI D G AL+I D G+Y+ VA N LG+A + V H +S + Q K + P + P+F D R V EG + + + P N L W +G + + + L ++L+I T +D+G Y C F++ G E+ + +II+ P+S + E +++E + DL P F+ Q++P E +++P ND +L V W K+ + N +R+QI + G +L++ ED+G Y + N +GQ QS
Sbjct: 804 EAPEFTGKFQSTTLYD-GDSVKLYCKA--AGEG-VSFKWFKDNEPISSGGSYAVDNKGNETTLHINNATMKEGGWYRCDATNKHGT---TTLKGRVVVNSRQKFNGPAHREMIT-LRKVDKVERSRTPVNQLQDVSASKSSPKFEGSL-QSQQLVEGQSARLEIKYTPVEDPNLRIAWLLNGKGILASSRIVTFTDFGIAALEINPVNVFDQGEYTVVAVNPLGEARVSANIAVIGHGSFIQQQQSGSQFGGTAYQSKGAQAPAGTHLDL---------PNFHS--DLRSQEVFEGQQIHLETKLTPINDPDLRVVWLLDGNELPSNDKYRQTLSHGFASLDIPQTSSNDSGLYSCRAFNKLGESEN---------QATIIIVPKSD----LQQFEQHRQLDVEDVREIQFAHSSQDLTPKFLSQIQPFHCEQELGRSFFEARIQPINDPTLRVSWLKDGQPLPNANRIQIFQNFGVVSLSLHPTYPEDAGVYTCVLFNSHGQAQS 1276 HSP 12 Score: 81.6481 bits (200), Expect = 2.060e-15 Identity = 109/509 (21.41%), Postives = 192/509 (37.72%), Query Frame = 3
Query: 666 AKTIPKFLSQLPNSLTLIQGQPLHIEIF-LEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLTV 2189
+K P +SQ L +QG+ F +E + + W KDG +K R + +L I G+Y+ N+ G E+ + N+T P + + + AR ND + + + PA F I G K + W+ +G+ + R E L + +D G Y V + G + LNV +K+ KG P+ E P F Q++PI ++EG + + D + + WY+N + + +IS +G A L I +A +ED +Y + A+N G+ S A+ +L +G + + ++ G +++Q+++ + P+F K +I+A +G KF +V G P PT++W+ + + K DG LE+ R + + + N G A +A LT+
Sbjct: 4426 SKQAPPTISQ---QLKPLQGELGKAAKFVIEFAGAAPVKVTWLKDGKEIKSTFRTLITTTPTNSSLHIGRLENSHAGEYTVRLENAAGTVESLA-NLTVAPPTTQGKAPDFS-----------------ARLND------------------LRIQQNGPAEFSCQI--GGEPKPTIQWFKDGQPLPNDDRFQVVEEGGAYKLKFSNIISTDAGIYEIVAKNGVGEARCK-ARLNVNLQKT------GKGAE---------EGPRYEA---------PRFTSQIQPI-VVDEGKGAQFSAQFSGFPDPT--IRWYRNNEPVKHADGYEISQSKGEAILRISAARNEDVAEYKVEASNPAGKASS--------------VANLVLTPRSG----RIAKSTISRGGSASYQSSDKAAADS-PHFIAKLSDISARQGHTVKFSAEVDGNPEPTVQWQFKGKPISASNNVKISRDGKRAILELARVTPDSAGEYQIVIRNDKGAATSQAKLTL 4846 HSP 13 Score: 58.5362 bits (140), Expect = 2.679e-8 Identity = 86/371 (23.18%), Postives = 133/371 (35.85%), Query Frame = 3
Query: 678 PKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQS-CNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTI----DPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSE-KINIS 1772
P F ++L N L + Q P E + + +WFKDG L R V+ + G L + D G Y VA+N +G+A ++ NV L+ EE RY P F I P I V EG A F DP + WY N E VK + L I D Y +VE+ + +++L P+S I+ + D+ D P FI +L I +G +V + +V+ + V+W I + ++IS D A L + + +G+Y ++ N G S+ K+ +S
Sbjct: 4530 PDFSARL-NDLRIQQNGP--AEFSCQIGGEPKPTIQWFKDGQPLPNDDRFQVVEEGGAYKLKFSNIISTDAGIYEIVAKNGVGEARCKARLNVN--------------------LQKTGKGAEEGPRYE-------APRFTSQIQP--IVVDEGKGAQFSAQFSGFPDP------TIRWYRNNEPVKHADGYEISQSKGEAILRISAARNEDVAEY---------KVEASNPAGKASSVANLVLTPRSGRIAKS---TISRGGSASYQSSDKAAADSPHFIAKLSDIS-ARQGHTVKFSAEVD--GNPEPTVQWQFKGKPISASNNVKISRDGKRAILELARVTPDSAGEYQIVIRNDKGAATSQAKLTLS 4847 HSP 14 Score: 58.5362 bits (140), Expect = 2.702e-8 Identity = 57/224 (25.45%), Postives = 93/224 (41.52%), Query Frame = 3
Query: 1530 LNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQ-EECLPNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLE--WRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLTVI 2192
L +GDSV L K A + +W+K+ I +G + TL+I +A ++ G Y ATNK+G T +G +V + A + + + D + + +DV + P F+ + EG A+ +K + P L W ++ ++ S T+ D I LEIN + + V A NPLG A+ A + VI
Sbjct: 817 LYDGDSVKLYCK---AAGEGVSFKWFKDNEPISSGGSYAVDNKGNETTLHINNATMKEGGWYRCDATNKHGTT--------TLKGRVVVNSRQKFNGPAHREMITLRKVDKVERSRTPVNQLQDVSASKSSPKFEGSLQSQQLVEGQSARLEIKYTPVEDPNLRIAWLLNGKGILASSRIVTFTDFGIAALEINPVNVFDQGEYTVVAVNPLGEARVSANIAVI 1029
BLAST of Titin vs. Ensembl Celegans
Match: ketn-1 (KETtiN (Drosophila actin-binding) homolog; Kettin [Source:UniProtKB/TrEMBL;Acc:Q9NL87]) HSP 1 Score: 237.269 bits (604), Expect = 2.492e-64 Identity = 162/517 (31.33%), Postives = 258/517 (49.90%), Query Frame = 3
Query: 255 DSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPM-PPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAI-----HALEAELNAPKAEMKEDEIITDLP----SFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISC 1775
++P F ++L ++ V E E + R+ PA+D + + W+ +G L A RF +FD G++ LD+LY D+G Y VA++ G A+SN+ V + E V YL E ++ I L+++ G E + PKFL+ LP+ + L + + +H+++ +D ++V EWF +G L GSR+ + + G+ ALDI ED+G YS A N LG+A Q C +T P I++ + ES + ++ LEN + Y R P PP+F+ P+ V EG P + + ++P N L W +G+ + R + +L+IL D G+Y C + G+ E T +I P+ + A+ +A E+ APK +E + DLP +F+KQL P Q EGD+V L +V P +DNSL EW N ++ R +S D G LNI+ ED+G Y L+ N+ G+ QS ++I+C
Sbjct: 1326 EAPKFARELAGKIEVMENERVHFEARILPANDVKMTVEWYHNGNPLPAAHRFHPMFDFGYVALDLLYAYPQDSGTYTLVARNELGEARSNVELV-VGTEK-VLYLEPHHPEGLERIKELEQDRRQGI---AEVEDRTCDAAPKFLNDLPD-IQLNEHENIHVDLRATPVNDPTMVIEWFVNGRPLLTGSRVKTLNEFGFIALDIKGAIAEDSGTYSVRASNLLGEAIRQ-CVITVTPAGQILSDTQHQES-LGKINYLENLNK-YGRVEIEDKGPQEPPTFVVPLQADLGDVEEGEPIHLECQVNPINDNSLKIIWLRDGQSLPHGHRFRTFYDFGFVSLDILGFYAQDAGTYTCRAENSLGQAE---------TVATIRCAPKDAILGAVQHPRSYARIQEIEAPKPAPQE---VPDLPHQPPAFVKQLGPAIQCMEGDNVYLEAQVTPTDDNSLTYEWLVNGQPLMKAHRFVLSQDFGYIALNILYCYPEDNGTYTLVVRNRAGEAQS-TVDINC 1820 HSP 2 Score: 222.246 bits (565), Expect = 2.190e-59 Identity = 162/551 (29.40%), Postives = 269/551 (48.82%), Query Frame = 3
Query: 252 YDSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESG----VDYLTQLPEEMMQEINNLDKNYETGTYHGMEE-DKHAAKTIPKFLSQLPNSLTLIQ-GQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQ-SKGISAIHALEAELNAPKAEMKED-EIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADS---AGYQRLKDLEEDLASG 1871
+ PTF ++L + EG+ +RL R+ P +D ++ + W ++G+ L SRF + ++ LDIL +D+G Y C A S G A ++ + +G +D + + +QEI N +K +E D KT PKF++QL +SL +Q G P+H+E +E +D+ L +WF +G L G R +D GY ALDI + + +D G+++CVARNSLG+A+T N T P +I T S E S +++ LE P D P FIEP+D I E A+F+ + P L W+ +G+ + +R + +L+I +T+P D+G Y + G E + L V I+ D Q + I +E AP+A +E+ ++ P F+ QL +D + EG + P +D V+WY N N + SR + D G +L++ + ED+G+Y ++ N G+ ++ +SC T +++L D+ +QR++++E A G
Sbjct: 2123 FQPPTFTENLADKEDAQEGQSIRLECRLIPVNDPTMRVTWTRNGQPLPEASRFMPARNFDYVNLDILALYGEDSGVYTCKAVSAFGEAATSCT---VKCAAGKSLLLDTMHDASWKRVQEIENREK---------LEAVDAEPEKTAPKFVTQLNSSLGELQEGVPIHLEAQVEPTNDNQLTVQWFHNGQPLANGHRFRTRHDFGYVALDILYAFAQDTGEWACVARNSLGEAQT-IANFTVLPRGTIYTDSQHPE-SWQKIQVLEAPRAAAPEKPDAEH--DAPQFIEPLD--SIDRMEFQSAHFQTKVTPQTDPNLRIQWFKDGQPLMNSNRFKLTTDFGYISLDIAHTVPEDSGVYSVKASNAKGDAEVQ-AQLTVTGNAVILGDTQHEQSWQKIQLIE----APRAPGEEEPDVKHGPPKFVTQLHSLDGVVEGQPSHFEAQFIPFSDPKTSVQWYLNGNPLSASSRRILRNDFGLVSLDLQYTLGEDAGEYSVVVKNSEGEDRTSG-QLSC------TTRAAILGDTQHEQSWQRIQEIEAPRAPG 2643 HSP 3 Score: 219.55 bits (558), Expect = 1.618e-58 Identity = 164/575 (28.52%), Postives = 273/575 (47.48%), Query Frame = 3
Query: 168 TMAPHALEVVNQVNKRSLASPRRGSDHLYDSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTE---SSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPI-DPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKG--ISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGN 1874
++ P +L+ + Q+ A P D +++P L+ + ++EG L T+ P D S+++ W KDG+ + +R+ + D GF VLDIL+ ++ D G+Y ++SG A ++ + ++ SG+ L E+ + + L+ N +E K A P F+ L + +G H E +D+ L +W+ DG LK GSRI I GY L+I+ YPEDNG+Y C A N +G+A T S +TC P+E II+ + L E ++ ++ +E P AR + PP F + P E+ EG A+ + + P +L +W+HNG+ V +R+ + L + P D+G++ C ++HG E L V + + QS I LE ++ PK ++ + PSF + L + QLNE D+ +EP D +L V+W N + I +R+ + + G ATL I I+ D+G+Y +ATN G S G+ V +S+ + Q+L D E++ G+
Sbjct: 3284 SLQPQSLDRIRQIEAGKPA-PEERPDQQFEAPAIVNALQVQGALEEGGSAHLQTQFTPVADPSIKVEWLKDGQPIFHSNRYKMVHDFGFAVLDILHLLKHDAGEYTFRVSNNSGEASTSTS-FEVSESSGL-ILQPQNEQKAKAVEILEDNLRRRPEEIEQELKEAT---PVFIEPLSAPVETEEGGRAHFTARYEPVNDNQLQVQWYHDGRPLKNGSRIKTINSFGYVVLEISPTYPEDNGEYICRAVNRVGEAVT-STKLTCTPKEGIISATQLPERMANAGRRIAEIEAP--RPAREDAPDADHGPPKFTSALAGPPEL--QEGQQAHLECQVTPVADPRLVIEWFHNGQPVNHTNRMKAIHDFGFVVLQLTPAEPQDSGTWTCRATNQHGSDEVS-TELKVVGGGGVSYEWQSTAERKERITELEDWIHRPKEDLNLPAVDYPAPSFSQGLTDLGQLNEADATAFVCVLEPIGDPTLRVQWEHNGHPIPYSNRISCTNEFGVATLLIKHLIAADAGEYKCVATNVKGSATS--------VGKIAVESSTQIDAPQVVQQLVDSVENILEGD 3838 HSP 4 Score: 218.394 bits (555), Expect = 4.200e-58 Identity = 193/660 (29.24%), Postives = 299/660 (45.30%), Query Frame = 3
Query: 261 PTFQQDLESEL-VVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYL------TQLPEEM--MQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPN-SLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQL-EPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSA---GYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMN-ITAGEGGPAKFLVKVSGFPTPTL--EWRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLTV 2189
PT + L + + V E + L L +V P DD +L W +G L A SR+ + D GF+ LDI Y I +D+G Y V + +G A+++ E VD L T PE + ++E+ L D AA P F QL + L +GQ +H++ ++ +D SL EWF DG L GSRI I+D GY L+ +PED G Y+C A N +G+A T + C P +I ++ ESS +++ +EN +E R + PP+F E + +E EG + + P N + W NG+ + SR + L+IL D+G Y C S G + T+ KS++LD S E E N K E + E P F+ QL + +L EG + L +VEP NDN L V+W+ N + NG R + +D G L+I+ A ++D+G++ +A N G+ Q+ I+ F ++ ++ DS +Q+++ LE A+ D + + P F ++P++ I E A F KV+ P L +W D ++ + K D L+I V A+N G A+ +A LTV
Sbjct: 1860 PTIAKPLPATIDSVHESQTLHLEAQVTPIDDNTLRYEWLFNGNPLKASSRYRVLNDFGFVSLDIDYIIAEDSGKYTLVVYNSAGRAETSC-------EFQVDRLKSILSDTAHPESLRRIREMEQLQP--------AKPSDDDAAAQPPVFTQQLTGPTEPLKEGQSVHMDCVVQPINDPSLRIEWFHDGRPLMFGSRIRTIHDFGYVGLEFLHIHPEDTGTYTCKATNLIGEA-TTDIFLECKPRRNIYLDTH-HESSWQKIQEIENRVDE--REPTPELTFQPPTFTENLADKE-DAQEGQSIRLECRLIPVNDPTMRVTWTRNGQPLPEASRFMPARNFDYVNLDILALYGEDSGVYTCKAVSAFGEAATS-CTVKCAAGKSLLLDTMHDA-SWKRVQEIE-NREKLEAVDAEPEKTAPKFVTQLNSSLGELQEGVPIHLEAQVEPTNDNQLTVQWFHNGQPLANGHRFRTRHDFGYVALDILYAFAQDTGEWACVARNSLGEAQT----IANF---TVLPRGTIYTDSQHPESWQKIQVLEAPRAAA-----PEKPDAEHDA-PQF-IEPLDSIDRMEFQSAHFQTKVTPQTDPNLRIQWFKDGQPLMNSNRFKLTTDFGYISLDIAHTVPEDSGVYSVKASNAKGDAEVQAQLTV 2482 HSP 5 Score: 197.978 bits (502), Expect = 1.625e-51 Identity = 175/657 (26.64%), Postives = 307/657 (46.73%), Query Frame = 3
Query: 15 NGAIFKCITRHKYSFIFNLDFIAMTTQESMEY-----NNNSNFNSVPDEGVIPNGT----TMAPHALEVVNQVNKRSLASPRRGSDHLYDSPTFQQDLESELVVD--EGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESII--TKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILD---PQS-KGISAIHALE--AELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNF 1928
NG F+ TRH + ++ LD + Q++ E+ N+ ++ + V+P GT + P + + + + A+P + D +D+P F + L+S +D E + T+V P D +L I WFKDG+ L +RF D G+I LDI +T+ +D+G Y A + G A+ A + + + + TQ E+ Q+I ++ G EE+ PKF++QL + +++GQP H E SD +W+ +G L SR + D G +LD+ + ED G+YS V +NS G+ T S ++C +I+ T+ + I ++++ PG E + PSF++P+ + EG+ A + + P N L W++N + + + ++ + +L I TG Y C +++ G + + VQ + ++LD P S + I + A++ A L+AP+ E ++ P +++ E + + EG +VTL VEP+ D L +EW N + N +R + Y+ G A L IV + DSG Y A N G+ S ++ EKI+ S ++R+++LE + E++ ++ PNF
Sbjct: 2304 NGHRFR--TRHDFGYV-ALDILYAFAQDTGEWACVARNSLGEAQTIANFTVLPRGTIYTDSQHPESWQKIQVLEAPRAAAPEK-PDAEHDAPQFIEPLDS---IDRMEFQSAHFQTKVTPQTDPNLRIQWFKDGQPLMNSNRFKLTTDFGYISLDIAHTVPEDSGVYSVKASNAKGDAEVQ-AQLTVTGNAVILGDTQH-EQSWQKIQLIEAPRAPG-----EEEPDVKHGPPKFVTQLHSLDGVVEGQPSHFEAQFIPFSDPKTSVQWYLNGNPLSASSRRILRNDFGLVSLDLQYTLGEDAGEYSVVVKNSEGEDRT-SGQLSCTTRAAILGDTQHEQSWQRIQEIEAPRAPGAE-----PEGPVYEKPSFVQPLQSVG-DLPEGSVALLEARLVPVNDPNLRVQWFYNDQPLMESNWISTSNDFGCVSLRIAPVYARHTGVYSCKAWNDSGNAVTS-ANVGVQGSEGLLLDTSHPASLQKIQELEAIDKYARLDAPEREYEK-------PQWVQGFENYENVGEGQTVTLHGLVEPSGDPHLRLEWLLNGTPLRNANRFRQEYEFGNAILTIVHVLPHDSGVYTCRAWNTQGEA-STSATVTTAGYEKILYDSQ---HPVSWERIQELEAP------KIVEEIEEIVQKEKPNF 2921 HSP 6 Score: 197.593 bits (501), Expect = 1.840e-51 Identity = 189/752 (25.13%), Postives = 321/752 (42.69%), Query Frame = 3
Query: 42 RHKYSFIF-NLDFIAMTTQESMEYNNNS-----------NFNSVPDEGVIPNGTTMAPHALEVVNQVNKRSLASPRRGSDHLYDSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRIN-PESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSL-TLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADS---AGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAG---EGGPAKFLVKVSGFPTP----TLEWRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLTVIPMVDFRPDLRH 2225
R Y F F +LD + Q++ Y + P + ++ G P + + ++ A P+ D + P F + L + EG+ + L +V P DD SL W +G+ L RF D G+I L+ILY +DNG Y V ++ +G AQS V IN +G ++ + I L+ + E K +P LP ++ ++ + Q LH+E + D++L EW +G LK SR V+ D G+ +LDI + ED+G Y+ V NS G+AET SC +SI++ + E S+ +++ +E + A+ +D PP F + + + EG + + P N L +W+H+G + SR+ + L L+ P DTG+Y C + G ++ + L + ++I LD + S+ ++ N E+ P+F + L + EG S+ L ++ P ND ++ V W +N + SR + + L+I++ EDSG Y A + G+ + + C G+ SLL D+ A ++R++++E N + + E+ P F V +N + G EG P +V PT T++W + + +T +D L+I + A N LG A+ A TV+P D +H
Sbjct: 1646 RTFYDFGFVSLDILGFYAQDAGTYTCRAENSLGQAETVATIRCAPKDAIL--GAVQHPRSYARIQEIEAPKPA-PQEVPDLPHQPPAFVKQLGPAIQCMEGDNVYLEAQVTPTDDNSLTYEWLVNGQPLMKAHRFVLSQDFGYIALNILYCYPEDNGTYTLVVRNRAGEAQST---VDINCGHTGGNFTDSFHPNSLHRIAELETPIQRA-----EPLPDKEKEVPTIAKPLPATIDSVHESQTLHLEAQVTPIDDNTLRYEWLFNGNPLKASSRYRVLNDFGFVSLDIDYIIAEDSGKYTLVVYNSAGRAET-SCEFQVDRLKSILSDTAHPE-SLRRIREMEQ--LQPAKPSDDDAAAQPPVFTQQLTGPTEPLKEGQSVHMDCVVQPINDPSLRIEWFHDGRPLMFGSRIRTIHDFGYVGLEFLHIHPEDTGTYTCKATNLIGEATTD-IFLECKPRRNIYLDTHHE--SSWQKIQEIENRVDEREPTPELTFQPPTFTENLADKEDAQEGQSIRLECRLIPVNDPTMRVTWTRNGQPLPEASRFMPARNFDYVNLDILALYGEDSGVYTCKAVSAFGEAAT-SCTVKCAAGK------SLLLDTMHDASWKRVQEIE------NREKLEAVDAEPEKTAPKF-VTQLNSSLGELQEGVPIHLEAQVE--PTNDNQLTVQWFHNGQPLANGHRFRTRHDFGYVALDILYAFAQDTGEWACVARNSLGEAQTIANFTVLPRGTIYTDSQH 2363 HSP 7 Score: 187.193 bits (474), Expect = 4.022e-48 Identity = 172/663 (25.94%), Postives = 282/663 (42.53%), Query Frame = 3
Query: 258 SPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGE-EYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAE---LNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEG-EKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNF--DVKPMNITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLTVIPMVDFRPDLRH 2225
SP F+ L+S+ +V EG+ RL + P +D +L I W +GK + A SR T D G L+I D G+Y VA + G A R++ V ++ Y++ G + +P F S L S + +GQ +H+E L +D L W DG L + G+ +LDI D+G YSC A N LG++E Q+ + I+ KS+L + + +E+ E ++A + + P F+ I P G ++F+ I P N L W +G+ + +R+ + +L++ T P D G Y CVLF+ HG+ +S V + + + + I L++ + E E+ + P F ++L ++ E + V ++ PAND + VEWY N N + R +D G L+++ A +DSG Y L+A N+ G+ +S N+ G EK++ + G +R+K+LE+D G ED + P F D+ + + E PT +EW ++ ++ S KT N+ L+I V A+N LG A + +TV P D +H
Sbjct: 936 SPKFEGSLQSQQLV-EGQSARLEIKYTPVEDPNLRIAWLLNGKGILASSRIVTFTDFGIAALEINPVNVFDQGEYTVVAVNPLGEA-------RVSANIAVIGHGSFIQQQQSGSQFGGTAYQS---KGAQAPAGTHLDLPNFHSDL-RSQEVFEGQQIHLETKLTPINDPDLRVVWLLDGNELPSNDKYRQTLSHGFASLDIPQTSSNDSGLYSCRAFNKLGESENQATII-------IVPKSDLQQFEQHRQLDVEDVREIQFAHSSQD----LTPKFLSQIQPFHCEQELGR-SFFEARIQPINDPTLRVSWLKDGQPLPNANRIQIFQNFGVVSLSLHPTYPEDAGVYTCVLFNSHGQAQSSAELTTVWIDTLQLDSKHADSLPIIGYLDSHQIHIGPQSVERPEEFNSLEAPKFARELAGKIEVMENERVHFEARILPANDVKMTVEWYHNGNPLPAAHRFHPMFDFGYVALDLLYAYPQDSGTYTLVARNELGEARS---NVELVVGTEKVLYLEPHHPE--GLERIKELEQDRRQG----IAEVEDRTCDAAPKFLNDLPDIQLNEHENIHVDLRATPVNDPTMVIEWFVNGRPLLTGSRVKTLNEFGFIALDIKGAIAEDSGTYSVRASNLLGEAIRQCVITVTPAGQILSDTQH 1565 HSP 8 Score: 186.808 bits (473), Expect = 5.828e-48 Identity = 146/580 (25.17%), Postives = 259/580 (44.66%), Query Frame = 3
Query: 249 LYDSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGV--DYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQS-KGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAGEGGPAKF 1979
+YD P ++ + +EG+ + + P +D L++ W ++G L+ GS+++ D G LD+ YT +D G Y + G A S+ A ++ + + + D Q + +QEI + +EE K P+F+ QL + +L++ QP H E +E D +L WF +G + SR+ +I D G+ +DIA P D+G++ CVA+N+ G+A + + +E +I + +L S+ +++ +E D P+ + + + ++ EG A+ + P + +W +G+ + +R + + L+IL+ L D G Y + + G S + V +IL PQ+ + A+ LE L + E E E+ P FI+ L + EG T + EP NDN L V+WY + + NGSR++ G L I ED+G+Y A N+ G+ + ++C E I++A+ L A R I+ + +E+ P+ D P T+ GP +
Sbjct: 3047 VYDHPAITTQIDDK-ECNEGDHVHFEALITPVNDPRLQVQWIRNGVPLAHGSKYAIQQDFGICTLDVAYTYPEDEGVYQLRIWNPEGEAVSS-ATLKCHGKDAILGDVQHQESWKRIQEIEAPKQK--------LEEADPEPKGPPRFIQQLTSPGSLVENQPAHFEATVEPVDDPTLTINWFLNGEPMSASSRVKMINDFGWVIMDIAQTEPRDSGEWKCVAKNAAGEA-VSTATIEVQGKE-VILQDSLQPQSLDRIRQIEAGKPAPEERPDQQF--EAPAIVNALQ-VQGALEEGGSAHLQTQFTPVADPSIKVEWLKDGQPIFHSNRYKMVHDFGFAVLDILHLLKHDAGEYTFRVSNNSGEA-STSTSFEVSESSGLILQPQNEQKAKAVEILEDNLRR-RPEEIEQELKEATPVFIEPLSAPVETEEGGRAHFTARYEPVNDNQLQVQWYHDGRPLKNGSRIKTINSFGYVVLEISPTYPEDNGEYICRAVNRVGEAVT-STKLTCTPKEGIISATQLPERMANAGR-----------RIAEIEAPRPAREDA-PDADHGPPKFTSALAGPPEL 3596 HSP 9 Score: 186.808 bits (473), Expect = 6.366e-48 Identity = 197/808 (24.38%), Postives = 336/808 (41.58%), Query Frame = 3
Query: 15 NGAIFKCITRHKYSFIF--------NLDFIAMTTQESMEYNNNSNFNSVPDEGVIPNGTTMAPHALEVVNQVNKRSLASPRR--GSDHLYDSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKT---IPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAEL---NAPKAEMKEDEIITD----LPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQ--EECLPNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLVVPDSVCKTYNDGSINYLEI--NRCSELGDIKVHVAATNPLGVAKEEATLTVIPMVDFRPDLRHITPENPFKKMAGLKKVECTSE------LNDAFKKS-KPTAERIVKIE 2345
NG+ K I Y + N ++I E ++ P EG+I + T + ++ + P R D + P F L + EG+ L +V P D L I WF +G+ ++ +R I D GF+VL + D+G + C A + G+ + + +++ GV Y Q E + I L+ H +ED + P F L + L + LE D +L +W +G + +RI + G L I D G+Y CVA N G A +S+ ++ ++ I P + +D E ++ EG + + + P N +L +W NG + SR E +L+ILY P D G Y V+ ++ G S+ + V S+ Q+ G + +LE+ + K ++ ++I + P F QL+ I L EG+ +V P ND L VEW+K++ ++ G R + + D G A L+++ A+ +D+G+Y +ATN++GQT ++C ++T S + R+ ++++D + NI + VQ ++ P F + N+ E PA+F V+G+P P + W I+ + K DG ++YL + +R S+ G+ V A N G ATL + DFR + P N FK L++ E + L AF+ + KP A++++ +E
Sbjct: 3493 NGSRIKTINSFGYVVLEISPTYPEDNGEYICRAVNRVGEAVTSTKLTCTPKEGII-SATQLPERMANAGRRIAEIEAPRPAREDAPDADHGPPKFTSALAGPPELQEGQQAHLECQVTPVADPRLVIEWFHNGQPVNHTNRMKAIHDFGFVVLQLTPAEPQDSGTWTCRATNQHGSDEVS-TELKVVGGGGVSYEWQSTAERKERITELED-----WIHRPKEDLNLPAVDYPAPSFSQGLTDLGQLNEADATAFVCVLEPIGDPTLRVQWEHNGHPIPYSNRISCTNEFGVATLLIKHLIAADAGEYKCVATNVKGSA-----------------------TSVGKIA-----------VESSTQIDAPQVVQQLVDSVE-NILEGDSIHLECRVTPINDPRLHVEWLRNGAPLPEASRFKPTFEFGFVSLDILYAYPEDNGDYELVVRNDKGEARSK-TKITVLPRPSLDYTSQTHG-NQQDSLESHFKQHSQAKLQLTANDIYNESDKRAPEFRTQLQNIGVL-EGEFCRFETQVAPINDPYLKVEWFKDKKPVLLGHRFRSTLDFGFACLDLLYALPDDTGEYHCVATNRHGQTMI-SAKLACQGASHVITDSQMPQGL----RVSNVKKD--NKNIYWSEQGGAVQPKQKQAPQFTIPLRNLQVTENQPARFECAVTGYPRPKVTWFINGNQCLHGHRFKLNFDG-LHYLTVSKSRISDAGE--VVAIARNTEGETISTATLDIFQNDDFR--QTKLRPAN-FKTSDELRERELQWQRDTLGSLGPAFEAAPKPDAQKLMHVE 4242 HSP 10 Score: 154.066 bits (388), Expect = 1.031e-37 Identity = 140/509 (27.50%), Postives = 223/509 (43.81%), Query Frame = 3
Query: 243 DHLYDSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALE----AELNAPKAEMKEDEIIT-DLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQS 1754
D + P F L S V EG+P + P D + W+ +G LSA SR D G + LD+ YT+ +D G+Y V K+ G +++ + + + TQ E+ Q I ++ G E + P F+ L + L +G +E L +D +L +WF + L + I D G +L IA Y G YSC A N G A T S NV E ++ ++ +S+ +++ LE ++YAR + P +++ + E +V EG ++P L +W NG ++ +R + E + L I++ LP D+G Y C ++ G + EK I+ D Q H + EL APK + +EI+ + P+F+ QLE + + EG + L +PA D L V W KN + +Q ++ G ATL+I SA + +G Y L TN G+ S
Sbjct: 2516 DVKHGPPKFVTQLHSLDGVVEGQPSHFEAQFIPFSDPKTSVQWYLNGNPLSASSRRILRNDFGLVSLDLQYTLGEDAGEYSVVVKNSEGEDRTS-GQLSCTTRAAILGDTQH-EQSWQRIQEIEAPRAPGA-----EPEGPVYEKPSFVQPLQSVGDLPEGSVALLEARLVPVNDPNLRVQWFYNDQPLMESNWISTSNDFGCVSLRIAPVYARHTGVYSCKAWNDSGNAVT-SANVGVQGSEGLLLDTS-HPASLQKIQELEAI-DKYARLDAPEREYEKPQWVQGFENYE-NVGEGQTVTLHGLVEPSGDPHLRLEWLLNGTPLRNANRFRQEYEFGNAILTIVHVLPHDSGVYTCRAWNTQGEASTSATVTTAGYEK-ILYDSQ-------HPVSWERIQELEAPKIVEEIEEIVQKEKPNFLTQLESAENVPEGVPLHLESTFQPARDPELKVVWQKNGQPLGASQLVQTKHELGWATLDISSANEDHNGVYTLTITNSEGEAVS 3005 HSP 11 Score: 118.627 bits (296), Expect = 1.249e-26 Identity = 127/506 (25.10%), Postives = 211/506 (41.70%), Query Frame = 3
Query: 255 DSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQS-CNVTCHPEESIITKSNLTESSIS-QLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEII---TDL-PSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQS 1754
++P F +S + D G+ ++L + A +G + WFKD + +S+G ++ L I + G Y C A + GT R+ S + EM+ + +DK + T +D A+K+ PKF L S L++GQ +EI D +L W +G + SRI D G AL+I D G+Y+ VA N LG+A + V H +S + Q K + P + P+F D R V EG + + + P N L W +G + + + L ++L+I T +D+G Y C F++ G E+ + +II+ P+S + E +++E + DL P F+ Q++P E +++P ND +L V W K+ + N +R+QI + G +L++ ED+G Y + N +GQ QS
Sbjct: 804 EAPEFTGKFQSTTLYD-GDSVKLYCKA--AGEG-VSFKWFKDNEPISSGGSYAVDNKGNETTLHINNATMKEGGWYRCDATNKHGT---TTLKGRVVVNSRQKFNGPAHREMIT-LRKVDKVERSRTPVNQLQDVSASKSSPKFEGSL-QSQQLVEGQSARLEIKYTPVEDPNLRIAWLLNGKGILASSRIVTFTDFGIAALEINPVNVFDQGEYTVVAVNPLGEARVSANIAVIGHGSFIQQQQSGSQFGGTAYQSKGAQAPAGTHLDL---------PNFHS--DLRSQEVFEGQQIHLETKLTPINDPDLRVVWLLDGNELPSNDKYRQTLSHGFASLDIPQTSSNDSGLYSCRAFNKLGESEN---------QATIIIVPKSD----LQQFEQHRQLDVEDVREIQFAHSSQDLTPKFLSQIQPFHCEQELGRSFFEARIQPINDPTLRVSWLKDGQPLPNANRIQIFQNFGVVSLSLHPTYPEDAGVYTCVLFNSHGQAQS 1276 HSP 12 Score: 81.6481 bits (200), Expect = 2.046e-15 Identity = 109/509 (21.41%), Postives = 192/509 (37.72%), Query Frame = 3
Query: 666 AKTIPKFLSQLPNSLTLIQGQPLHIEIF-LEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLTV 2189
+K P +SQ L +QG+ F +E + + W KDG +K R + +L I G+Y+ N+ G E+ + N+T P + + + AR ND + + + PA F I G K + W+ +G+ + R E L + +D G Y V + G + LNV +K+ KG P+ E P F Q++PI ++EG + + D + + WY+N + + +IS +G A L I +A +ED +Y + A+N G+ S A+ +L +G + + ++ G +++Q+++ + P+F K +I+A +G KF +V G P PT++W+ + + K DG LE+ R + + + N G A +A LT+
Sbjct: 4541 SKQAPPTISQ---QLKPLQGELGKAAKFVIEFAGAAPVKVTWLKDGKEIKSTFRTLITTTPTNSSLHIGRLENSHAGEYTVRLENAAGTVESLA-NLTVAPPTTQGKAPDFS-----------------ARLND------------------LRIQQNGPAEFSCQI--GGEPKPTIQWFKDGQPLPNDDRFQVVEEGGAYKLKFSNIISTDAGIYEIVAKNGVGEARCK-ARLNVNLQKT------GKGAE---------EGPRYEA---------PRFTSQIQPI-VVDEGKGAQFSAQFSGFPDPT--IRWYRNNEPVKHADGYEISQSKGEAILRISAARNEDVAEYKVEASNPAGKASS--------------VANLVLTPRSG----RIAKSTISRGGSASYQSSDKAAADS-PHFIAKLSDISARQGHTVKFSAEVDGNPEPTVQWQFKGKPISASNNVKISRDGKRAILELARVTPDSAGEYQIVIRNDKGAATSQAKLTL 4961 HSP 13 Score: 58.5362 bits (140), Expect = 2.549e-8 Identity = 57/224 (25.45%), Postives = 93/224 (41.52%), Query Frame = 3
Query: 1530 LNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQ-EECLPNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLE--WRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLTVI 2192
L +GDSV L K A + +W+K+ I +G + TL+I +A ++ G Y ATNK+G T +G +V + A + + + D + + +DV + P F+ + EG A+ +K + P L W ++ ++ S T+ D I LEIN + + V A NPLG A+ A + VI
Sbjct: 817 LYDGDSVKLYCK---AAGEGVSFKWFKDNEPISSGGSYAVDNKGNETTLHINNATMKEGGWYRCDATNKHGTT--------TLKGRVVVNSRQKFNGPAHREMITLRKVDKVERSRTPVNQLQDVSASKSSPKFEGSLQSQQLVEGQSARLEIKYTPVEDPNLRIAWLLNGKGILASSRIVTFTDFGIAALEINPVNVFDQGEYTVVAVNPLGEARVSANIAVI 1029 HSP 14 Score: 58.5362 bits (140), Expect = 2.798e-8 Identity = 86/371 (23.18%), Postives = 133/371 (35.85%), Query Frame = 3
Query: 678 PKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQS-CNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTI----DPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSE-KINIS 1772
P F ++L N L + Q P E + + +WFKDG L R V+ + G L + D G Y VA+N +G+A ++ NV L+ EE RY P F I P I V EG A F DP + WY N E VK + L I D Y +VE+ + +++L P+S I+ + D+ D P FI +L I +G +V + +V+ + V+W I + ++IS D A L + + +G+Y ++ N G S+ K+ +S
Sbjct: 4645 PDFSARL-NDLRIQQNGP--AEFSCQIGGEPKPTIQWFKDGQPLPNDDRFQVVEEGGAYKLKFSNIISTDAGIYEIVAKNGVGEARCKARLNVN--------------------LQKTGKGAEEGPRYE-------APRFTSQIQP--IVVDEGKGAQFSAQFSGFPDP------TIRWYRNNEPVKHADGYEISQSKGEAILRISAARNEDVAEY---------KVEASNPAGKASSVANLVLTPRSGRIAKS---TISRGGSASYQSSDKAAADSPHFIAKLSDIS-ARQGHTVKFSAEVD--GNPEPTVQWQFKGKPISASNNVKISRDGKRAILELARVTPDSAGEYQIVIRNDKGAATSQAKLTLS 4962
BLAST of Titin vs. Ensembl Celegans
Match: ketn-1 (KETtiN (Drosophila actin-binding) homolog; Kettin [Source:UniProtKB/TrEMBL;Acc:Q9NL87]) HSP 1 Score: 237.269 bits (604), Expect = 2.925e-64 Identity = 162/517 (31.33%), Postives = 258/517 (49.90%), Query Frame = 3
Query: 255 DSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPM-PPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAI-----HALEAELNAPKAEMKEDEIITDLP----SFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISC 1775
++P F ++L ++ V E E + R+ PA+D + + W+ +G L A RF +FD G++ LD+LY D+G Y VA++ G A+SN+ V + E V YL E ++ I L+++ G E + PKFL+ LP+ + L + + +H+++ +D ++V EWF +G L GSR+ + + G+ ALDI ED+G YS A N LG+A Q C +T P I++ + ES + ++ LEN + Y R P PP+F+ P+ V EG P + + ++P N L W +G+ + R + +L+IL D G+Y C + G+ E T +I P+ + A+ +A E+ APK +E + DLP +F+KQL P Q EGD+V L +V P +DNSL EW N ++ R +S D G LNI+ ED+G Y L+ N+ G+ QS ++I+C
Sbjct: 687 EAPKFARELAGKIEVMENERVHFEARILPANDVKMTVEWYHNGNPLPAAHRFHPMFDFGYVALDLLYAYPQDSGTYTLVARNELGEARSNVELV-VGTEK-VLYLEPHHPEGLERIKELEQDRRQGI---AEVEDRTCDAAPKFLNDLPD-IQLNEHENIHVDLRATPVNDPTMVIEWFVNGRPLLTGSRVKTLNEFGFIALDIKGAIAEDSGTYSVRASNLLGEAIRQ-CVITVTPAGQILSDTQHQES-LGKINYLENLNK-YGRVEIEDKGPQEPPTFVVPLQADLGDVEEGEPIHLECQVNPINDNSLKIIWLRDGQSLPHGHRFRTFYDFGFVSLDILGFYAQDAGTYTCRAENSLGQAE---------TVATIRCAPKDAILGAVQHPRSYARIQEIEAPKPAPQE---VPDLPHQPPAFVKQLGPAIQCMEGDNVYLEAQVTPTDDNSLTYEWLVNGQPLMKAHRFVLSQDFGYIALNILYCYPEDNGTYTLVVRNRAGEAQS-TVDINC 1181 HSP 2 Score: 222.246 bits (565), Expect = 2.347e-59 Identity = 162/551 (29.40%), Postives = 269/551 (48.82%), Query Frame = 3
Query: 252 YDSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESG----VDYLTQLPEEMMQEINNLDKNYETGTYHGMEE-DKHAAKTIPKFLSQLPNSLTLIQ-GQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQ-SKGISAIHALEAELNAPKAEMKED-EIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADS---AGYQRLKDLEEDLASG 1871
+ PTF ++L + EG+ +RL R+ P +D ++ + W ++G+ L SRF + ++ LDIL +D+G Y C A S G A ++ + +G +D + + +QEI N +K +E D KT PKF++QL +SL +Q G P+H+E +E +D+ L +WF +G L G R +D GY ALDI + + +D G+++CVARNSLG+A+T N T P +I T S E S +++ LE P D P FIEP+D I E A+F+ + P L W+ +G+ + +R + +L+I +T+P D+G Y + G E + L V I+ D Q + I +E AP+A +E+ ++ P F+ QL +D + EG + P +D V+WY N N + SR + D G +L++ + ED+G+Y ++ N G+ ++ +SC T +++L D+ +QR++++E A G
Sbjct: 1484 FQPPTFTENLADKEDAQEGQSIRLECRLIPVNDPTMRVTWTRNGQPLPEASRFMPARNFDYVNLDILALYGEDSGVYTCKAVSAFGEAATSCT---VKCAAGKSLLLDTMHDASWKRVQEIENREK---------LEAVDAEPEKTAPKFVTQLNSSLGELQEGVPIHLEAQVEPTNDNQLTVQWFHNGQPLANGHRFRTRHDFGYVALDILYAFAQDTGEWACVARNSLGEAQT-IANFTVLPRGTIYTDSQHPE-SWQKIQVLEAPRAAAPEKPDAEH--DAPQFIEPLD--SIDRMEFQSAHFQTKVTPQTDPNLRIQWFKDGQPLMNSNRFKLTTDFGYISLDIAHTVPEDSGVYSVKASNAKGDAEVQ-AQLTVTGNAVILGDTQHEQSWQKIQLIE----APRAPGEEEPDVKHGPPKFVTQLHSLDGVVEGQPSHFEAQFIPFSDPKTSVQWYLNGNPLSASSRRILRNDFGLVSLDLQYTLGEDAGEYSVVVKNSEGEDRTSG-QLSC------TTRAAILGDTQHEQSWQRIQEIEAPRAPG 2004 HSP 3 Score: 219.55 bits (558), Expect = 1.448e-58 Identity = 164/575 (28.52%), Postives = 273/575 (47.48%), Query Frame = 3
Query: 168 TMAPHALEVVNQVNKRSLASPRRGSDHLYDSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTE---SSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPI-DPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKG--ISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGN 1874
++ P +L+ + Q+ A P D +++P L+ + ++EG L T+ P D S+++ W KDG+ + +R+ + D GF VLDIL+ ++ D G+Y ++SG A ++ + ++ SG+ L E+ + + L+ N +E K A P F+ L + +G H E +D+ L +W+ DG LK GSRI I GY L+I+ YPEDNG+Y C A N +G+A T S +TC P+E II+ + L E ++ ++ +E P AR + PP F + P E+ EG A+ + + P +L +W+HNG+ V +R+ + L + P D+G++ C ++HG E L V + + QS I LE ++ PK ++ + PSF + L + QLNE D+ +EP D +L V+W N + I +R+ + + G ATL I I+ D+G+Y +ATN G S G+ V +S+ + Q+L D E++ G+
Sbjct: 2645 SLQPQSLDRIRQIEAGKPA-PEERPDQQFEAPAIVNALQVQGALEEGGSAHLQTQFTPVADPSIKVEWLKDGQPIFHSNRYKMVHDFGFAVLDILHLLKHDAGEYTFRVSNNSGEASTSTS-FEVSESSGL-ILQPQNEQKAKAVEILEDNLRRRPEEIEQELKEAT---PVFIEPLSAPVETEEGGRAHFTARYEPVNDNQLQVQWYHDGRPLKNGSRIKTINSFGYVVLEISPTYPEDNGEYICRAVNRVGEAVT-STKLTCTPKEGIISATQLPERMANAGRRIAEIEAP--RPAREDAPDADHGPPKFTSALAGPPEL--QEGQQAHLECQVTPVADPRLVIEWFHNGQPVNHTNRMKAIHDFGFVVLQLTPAEPQDSGTWTCRATNQHGSDEVS-TELKVVGGGGVSYEWQSTAERKERITELEDWIHRPKEDLNLPAVDYPAPSFSQGLTDLGQLNEADATAFVCVLEPIGDPTLRVQWEHNGHPIPYSNRISCTNEFGVATLLIKHLIAADAGEYKCVATNVKGSATS--------VGKIAVESSTQIDAPQVVQQLVDSVENILEGD 3199 HSP 4 Score: 218.009 bits (554), Expect = 5.635e-58 Identity = 189/654 (28.90%), Postives = 299/654 (45.72%), Query Frame = 3
Query: 261 PTFQQDLESEL-VVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEM--MQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPN-SLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQL-EPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSA---GYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMN-ITAGEGGPAKFLVKVSGFPTPTL--EWRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLTV 2189
PT + L + + V E + L L +V P DD +L W +G L A SR+ + D GF+ LDI Y I +D+G Y V + +G A+++ +++ + T PE + ++E+ L D AA P F QL + L +GQ +H++ ++ +D SL EWF DG L GSRI I+D GY L+ +PED G Y+C A N +G+A T + C P +I ++ ESS +++ +EN +E R + PP+F E + +E EG + + P N + W NG+ + SR + L+IL D+G Y C S G + T+ KS++LD S E E N K E + E P F+ QL + +L EG + L +VEP NDN L V+W+ N + NG R + +D G L+I+ A ++D+G++ +A N G+ Q+ I+ F ++ ++ DS +Q+++ LE A+ D + + P F ++P++ I E A F KV+ P L +W D ++ + K D L+I V A+N G A+ +A LTV
Sbjct: 1221 PTIAKPLPATIDSVHESQTLHLEAQVTPIDDNTLRYEWLFNGNPLKASSRYRVLNDFGFVSLDIDYIIAEDSGKYTLVVYNSAGRAETS-CEFQVDRLKSILSDTAHPESLRRIREMEQLQP--------AKPSDDDAAAQPPVFTQQLTGPTEPLKEGQSVHMDCVVQPINDPSLRIEWFHDGRPLMFGSRIRTIHDFGYVGLEFLHIHPEDTGTYTCKATNLIGEA-TTDIFLECKPRRNIYLDTH-HESSWQKIQEIENRVDE--REPTPELTFQPPTFTENLADKE-DAQEGQSIRLECRLIPVNDPTMRVTWTRNGQPLPEASRFMPARNFDYVNLDILALYGEDSGVYTCKAVSAFGEAATS-CTVKCAAGKSLLLDTMHDA-SWKRVQEIE-NREKLEAVDAEPEKTAPKFVTQLNSSLGELQEGVPIHLEAQVEPTNDNQLTVQWFHNGQPLANGHRFRTRHDFGYVALDILYAFAQDTGEWACVARNSLGEAQT----IANF---TVLPRGTIYTDSQHPESWQKIQVLEAPRAAA-----PEKPDAEHDA-PQF-IEPLDSIDRMEFQSAHFQTKVTPQTDPNLRIQWFKDGQPLMNSNRFKLTTDFGYISLDIAHTVPEDSGVYSVKASNAKGDAEVQAQLTV 1843 HSP 5 Score: 197.978 bits (502), Expect = 1.419e-51 Identity = 175/657 (26.64%), Postives = 307/657 (46.73%), Query Frame = 3
Query: 15 NGAIFKCITRHKYSFIFNLDFIAMTTQESMEY-----NNNSNFNSVPDEGVIPNGT----TMAPHALEVVNQVNKRSLASPRRGSDHLYDSPTFQQDLESELVVD--EGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESII--TKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILD---PQS-KGISAIHALE--AELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNF 1928
NG F+ TRH + ++ LD + Q++ E+ N+ ++ + V+P GT + P + + + + A+P + D +D+P F + L+S +D E + T+V P D +L I WFKDG+ L +RF D G+I LDI +T+ +D+G Y A + G A+ A + + + + TQ E+ Q+I ++ G EE+ PKF++QL + +++GQP H E SD +W+ +G L SR + D G +LD+ + ED G+YS V +NS G+ T S ++C +I+ T+ + I ++++ PG E + PSF++P+ + EG+ A + + P N L W++N + + + ++ + +L I TG Y C +++ G + + VQ + ++LD P S + I + A++ A L+AP+ E ++ P +++ E + + EG +VTL VEP+ D L +EW N + N +R + Y+ G A L IV + DSG Y A N G+ S ++ EKI+ S ++R+++LE + E++ ++ PNF
Sbjct: 1665 NGHRFR--TRHDFGYV-ALDILYAFAQDTGEWACVARNSLGEAQTIANFTVLPRGTIYTDSQHPESWQKIQVLEAPRAAAPEK-PDAEHDAPQFIEPLDS---IDRMEFQSAHFQTKVTPQTDPNLRIQWFKDGQPLMNSNRFKLTTDFGYISLDIAHTVPEDSGVYSVKASNAKGDAEVQ-AQLTVTGNAVILGDTQH-EQSWQKIQLIEAPRAPG-----EEEPDVKHGPPKFVTQLHSLDGVVEGQPSHFEAQFIPFSDPKTSVQWYLNGNPLSASSRRILRNDFGLVSLDLQYTLGEDAGEYSVVVKNSEGEDRT-SGQLSCTTRAAILGDTQHEQSWQRIQEIEAPRAPGAE-----PEGPVYEKPSFVQPLQSVG-DLPEGSVALLEARLVPVNDPNLRVQWFYNDQPLMESNWISTSNDFGCVSLRIAPVYARHTGVYSCKAWNDSGNAVTS-ANVGVQGSEGLLLDTSHPASLQKIQELEAIDKYARLDAPEREYEK-------PQWVQGFENYENVGEGQTVTLHGLVEPSGDPHLRLEWLLNGTPLRNANRFRQEYEFGNAILTIVHVLPHDSGVYTCRAWNTQGEA-STSATVTTAGYEKILYDSQ---HPVSWERIQELEAP------KIVEEIEEIVQKEKPNF 2282 HSP 6 Score: 197.978 bits (502), Expect = 1.695e-51 Identity = 189/752 (25.13%), Postives = 321/752 (42.69%), Query Frame = 3
Query: 42 RHKYSFIF-NLDFIAMTTQESMEYNNNS-----------NFNSVPDEGVIPNGTTMAPHALEVVNQVNKRSLASPRRGSDHLYDSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRIN-PESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSL-TLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADS---AGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAG---EGGPAKFLVKVSGFPTP----TLEWRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLTVIPMVDFRPDLRH 2225
R Y F F +LD + Q++ Y + P + ++ G P + + ++ A P+ D + P F + L + EG+ + L +V P DD SL W +G+ L RF D G+I L+ILY +DNG Y V ++ +G AQS V IN +G ++ + I L+ + E K +P LP ++ ++ + Q LH+E + D++L EW +G LK SR V+ D G+ +LDI + ED+G Y+ V NS G+AET SC +SI++ + E S+ +++ +E + A+ +D PP F + + + EG + + P N L +W+H+G + SR+ + L L+ P DTG+Y C + G ++ + L + ++I LD + S+ ++ N E+ P+F + L + EG S+ L ++ P ND ++ V W +N + SR + + L+I++ EDSG Y A + G+ + + C G+ SLL D+ A ++R++++E N + + E+ P F V +N + G EG P +V PT T++W + + +T +D L+I + A N LG A+ A TV+P D +H
Sbjct: 1007 RTFYDFGFVSLDILGFYAQDAGTYTCRAENSLGQAETVATIRCAPKDAIL--GAVQHPRSYARIQEIEAPKPA-PQEVPDLPHQPPAFVKQLGPAIQCMEGDNVYLEAQVTPTDDNSLTYEWLVNGQPLMKAHRFVLSQDFGYIALNILYCYPEDNGTYTLVVRNRAGEAQST---VDINCGHTGGNFTDSFHPNSLHRIAELETPIQRA-----EPLPDKEKEVPTIAKPLPATIDSVHESQTLHLEAQVTPIDDNTLRYEWLFNGNPLKASSRYRVLNDFGFVSLDIDYIIAEDSGKYTLVVYNSAGRAET-SCEFQVDRLKSILSDTAHPE-SLRRIREMEQ--LQPAKPSDDDAAAQPPVFTQQLTGPTEPLKEGQSVHMDCVVQPINDPSLRIEWFHDGRPLMFGSRIRTIHDFGYVGLEFLHIHPEDTGTYTCKATNLIGEATTD-IFLECKPRRNIYLDTHHE--SSWQKIQEIENRVDEREPTPELTFQPPTFTENLADKEDAQEGQSIRLECRLIPVNDPTMRVTWTRNGQPLPEASRFMPARNFDYVNLDILALYGEDSGVYTCKAVSAFGEAAT-SCTVKCAAGK------SLLLDTMHDASWKRVQEIE------NREKLEAVDAEPEKTAPKF-VTQLNSSLGELQEGVPIHLEAQVE--PTNDNQLTVQWFHNGQPLANGHRFRTRHDFGYVALDILYAFAQDTGEWACVARNSLGEAQTIANFTVLPRGTIYTDSQH 1724 HSP 7 Score: 187.193 bits (474), Expect = 4.625e-48 Identity = 172/663 (25.94%), Postives = 282/663 (42.53%), Query Frame = 3
Query: 258 SPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGE-EYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAE---LNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEG-EKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNF--DVKPMNITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLTVIPMVDFRPDLRH 2225
SP F+ L+S+ +V EG+ RL + P +D +L I W +GK + A SR T D G L+I D G+Y VA + G A R++ V ++ Y++ G + +P F S L S + +GQ +H+E L +D L W DG L + G+ +LDI D+G YSC A N LG++E Q+ + I+ KS+L + + +E+ E ++A + + P F+ I P G ++F+ I P N L W +G+ + +R+ + +L++ T P D G Y CVLF+ HG+ +S V + + + + I L++ + E E+ + P F ++L ++ E + V ++ PAND + VEWY N N + R +D G L+++ A +DSG Y L+A N+ G+ +S N+ G EK++ + G +R+K+LE+D G ED + P F D+ + + E PT +EW ++ ++ S KT N+ L+I V A+N LG A + +TV P D +H
Sbjct: 297 SPKFEGSLQSQQLV-EGQSARLEIKYTPVEDPNLRIAWLLNGKGILASSRIVTFTDFGIAALEINPVNVFDQGEYTVVAVNPLGEA-------RVSANIAVIGHGSFIQQQQSGSQFGGTAYQS---KGAQAPAGTHLDLPNFHSDL-RSQEVFEGQQIHLETKLTPINDPDLRVVWLLDGNELPSNDKYRQTLSHGFASLDIPQTSSNDSGLYSCRAFNKLGESENQATII-------IVPKSDLQQFEQHRQLDVEDVREIQFAHSSQD----LTPKFLSQIQPFHCEQELGR-SFFEARIQPINDPTLRVSWLKDGQPLPNANRIQIFQNFGVVSLSLHPTYPEDAGVYTCVLFNSHGQAQSSAELTTVWIDTLQLDSKHADSLPIIGYLDSHQIHIGPQSVERPEEFNSLEAPKFARELAGKIEVMENERVHFEARILPANDVKMTVEWYHNGNPLPAAHRFHPMFDFGYVALDLLYAYPQDSGTYTLVARNELGEARS---NVELVVGTEKVLYLEPHHPE--GLERIKELEQDRRQG----IAEVEDRTCDAAPKFLNDLPDIQLNEHENIHVDLRATPVNDPTMVIEWFVNGRPLLTGSRVKTLNEFGFIALDIKGAIAEDSGTYSVRASNLLGEAIRQCVITVTPAGQILSDTQH 926 HSP 8 Score: 186.422 bits (472), Expect = 6.469e-48 Identity = 146/580 (25.17%), Postives = 259/580 (44.66%), Query Frame = 3
Query: 249 LYDSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGV--DYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQS-KGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAGEGGPAKF 1979
+YD P ++ + +EG+ + + P +D L++ W ++G L+ GS+++ D G LD+ YT +D G Y + G A S+ A ++ + + + D Q + +QEI + +EE K P+F+ QL + +L++ QP H E +E D +L WF +G + SR+ +I D G+ +DIA P D+G++ CVA+N+ G+A + + +E +I + +L S+ +++ +E D P+ + + + ++ EG A+ + P + +W +G+ + +R + + L+IL+ L D G Y + + G S + V +IL PQ+ + A+ LE L + E E E+ P FI+ L + EG T + EP NDN L V+WY + + NGSR++ G L I ED+G+Y A N+ G+ + ++C E I++A+ L A R I+ + +E+ P+ D P T+ GP +
Sbjct: 2408 VYDHPAITTQIDDK-ECNEGDHVHFEALITPVNDPRLQVQWIRNGVPLAHGSKYAIQQDFGICTLDVAYTYPEDEGVYQLRIWNPEGEAVSS-ATLKCHGKDAILGDVQHQESWKRIQEIEAPKQK--------LEEADPEPKGPPRFIQQLTSPGSLVENQPAHFEATVEPVDDPTLTINWFLNGEPMSASSRVKMINDFGWVIMDIAQTEPRDSGEWKCVAKNAAGEA-VSTATIEVQGKE-VILQDSLQPQSLDRIRQIEAGKPAPEERPDQQF--EAPAIVNALQ-VQGALEEGGSAHLQTQFTPVADPSIKVEWLKDGQPIFHSNRYKMVHDFGFAVLDILHLLKHDAGEYTFRVSNNSGEA-STSTSFEVSESSGLILQPQNEQKAKAVEILEDNLRR-RPEEIEQELKEATPVFIEPLSAPVETEEGGRAHFTARYEPVNDNQLQVQWYHDGRPLKNGSRIKTINSFGYVVLEISPTYPEDNGEYICRAVNRVGEAVT-STKLTCTPKEGIISATQLPERMANAGR-----------RIAEIEAPRPAREDA-PDADHGPPKFTSALAGPPEL 2957 HSP 9 Score: 186.422 bits (472), Expect = 6.761e-48 Identity = 197/808 (24.38%), Postives = 336/808 (41.58%), Query Frame = 3
Query: 15 NGAIFKCITRHKYSFIF--------NLDFIAMTTQESMEYNNNSNFNSVPDEGVIPNGTTMAPHALEVVNQVNKRSLASPRR--GSDHLYDSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKT---IPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAEL---NAPKAEMKEDEIITD----LPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQ--EECLPNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLVVPDSVCKTYNDGSINYLEI--NRCSELGDIKVHVAATNPLGVAKEEATLTVIPMVDFRPDLRHITPENPFKKMAGLKKVECTSE------LNDAFKKS-KPTAERIVKIE 2345
NG+ K I Y + N ++I E ++ P EG+I + T + ++ + P R D + P F L + EG+ L +V P D L I WF +G+ ++ +R I D GF+VL + D+G + C A + G+ + + +++ GV Y Q E + I L+ H +ED + P F L + L + LE D +L +W +G + +RI + G L I D G+Y CVA N G A +S+ ++ ++ I P + +D E ++ EG + + + P N +L +W NG + SR E +L+ILY P D G Y V+ ++ G S+ + V S+ Q+ G + +LE+ + K ++ ++I + P F QL+ I L EG+ +V P ND L VEW+K++ ++ G R + + D G A L+++ A+ +D+G+Y +ATN++GQT ++C ++T S + R+ ++++D + NI + VQ ++ P F + N+ E PA+F V+G+P P + W I+ + K DG ++YL + +R S+ G+ V A N G ATL + DFR + P N FK L++ E + L AF+ + KP A++++ +E
Sbjct: 2854 NGSRIKTINSFGYVVLEISPTYPEDNGEYICRAVNRVGEAVTSTKLTCTPKEGII-SATQLPERMANAGRRIAEIEAPRPAREDAPDADHGPPKFTSALAGPPELQEGQQAHLECQVTPVADPRLVIEWFHNGQPVNHTNRMKAIHDFGFVVLQLTPAEPQDSGTWTCRATNQHGSDEVS-TELKVVGGGGVSYEWQSTAERKERITELED-----WIHRPKEDLNLPAVDYPAPSFSQGLTDLGQLNEADATAFVCVLEPIGDPTLRVQWEHNGHPIPYSNRISCTNEFGVATLLIKHLIAADAGEYKCVATNVKGSA-----------------------TSVGKIA-----------VESSTQIDAPQVVQQLVDSVE-NILEGDSIHLECRVTPINDPRLHVEWLRNGAPLPEASRFKPTFEFGFVSLDILYAYPEDNGDYELVVRNDKGEARSK-TKITVLPRPSLDYTSQTHG-NQQDSLESHFKQHSQAKLQLTANDIYNESDKRAPEFRTQLQNIGVL-EGEFCRFETQVAPINDPYLKVEWFKDKKPVLLGHRFRSTLDFGFACLDLLYALPDDTGEYHCVATNRHGQTMI-SAKLACQGASHVITDSQMPQGL----RVSNVKKD--NKNIYWSEQGGAVQPKQKQAPQFTIPLRNLQVTENQPARFECAVTGYPRPKVTWFINGNQCLHGHRFKLNFDG-LHYLTVSKSRISDAGE--VVAIARNTEGETISTATLDIFQNDDFR--QTKLRPAN-FKTSDELRERELQWQRDTLGSLGPAFEAAPKPDAQKLMHVE 3603 HSP 10 Score: 154.066 bits (388), Expect = 1.060e-37 Identity = 140/509 (27.50%), Postives = 223/509 (43.81%), Query Frame = 3
Query: 243 DHLYDSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALE----AELNAPKAEMKEDEIIT-DLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQS 1754
D + P F L S V EG+P + P D + W+ +G LSA SR D G + LD+ YT+ +D G+Y V K+ G +++ + + + TQ E+ Q I ++ G E + P F+ L + L +G +E L +D +L +WF + L + I D G +L IA Y G YSC A N G A T S NV E ++ ++ +S+ +++ LE ++YAR + P +++ + E +V EG ++P L +W NG ++ +R + E + L I++ LP D+G Y C ++ G + EK I+ D Q H + EL APK + +EI+ + P+F+ QLE + + EG + L +PA D L V W KN + +Q ++ G ATL+I SA + +G Y L TN G+ S
Sbjct: 1877 DVKHGPPKFVTQLHSLDGVVEGQPSHFEAQFIPFSDPKTSVQWYLNGNPLSASSRRILRNDFGLVSLDLQYTLGEDAGEYSVVVKNSEGEDRTS-GQLSCTTRAAILGDTQH-EQSWQRIQEIEAPRAPGA-----EPEGPVYEKPSFVQPLQSVGDLPEGSVALLEARLVPVNDPNLRVQWFYNDQPLMESNWISTSNDFGCVSLRIAPVYARHTGVYSCKAWNDSGNAVT-SANVGVQGSEGLLLDTS-HPASLQKIQELEAI-DKYARLDAPEREYEKPQWVQGFENYE-NVGEGQTVTLHGLVEPSGDPHLRLEWLLNGTPLRNANRFRQEYEFGNAILTIVHVLPHDSGVYTCRAWNTQGEASTSATVTTAGYEK-ILYDSQ-------HPVSWERIQELEAPKIVEEIEEIVQKEKPNFLTQLESAENVPEGVPLHLESTFQPARDPELKVVWQKNGQPLGASQLVQTKHELGWATLDISSANEDHNGVYTLTITNSEGEAVS 2366 HSP 11 Score: 118.242 bits (295), Expect = 1.268e-26 Identity = 127/506 (25.10%), Postives = 211/506 (41.70%), Query Frame = 3
Query: 255 DSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQS-CNVTCHPEESIITKSNLTESSIS-QLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEII---TDL-PSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQS 1754
++P F +S + D G+ ++L + A +G + WFKD + +S+G ++ L I + G Y C A + GT R+ S + EM+ + +DK + T +D A+K+ PKF L S L++GQ +EI D +L W +G + SRI D G AL+I D G+Y+ VA N LG+A + V H +S + Q K + P + P+F D R V EG + + + P N L W +G + + + L ++L+I T +D+G Y C F++ G E+ + +II+ P+S + E +++E + DL P F+ Q++P E +++P ND +L V W K+ + N +R+QI + G +L++ ED+G Y + N +GQ QS
Sbjct: 165 EAPEFTGKFQSTTLYD-GDSVKLYCKA--AGEG-VSFKWFKDNEPISSGGSYAVDNKGNETTLHINNATMKEGGWYRCDATNKHGT---TTLKGRVVVNSRQKFNGPAHREMIT-LRKVDKVERSRTPVNQLQDVSASKSSPKFEGSL-QSQQLVEGQSARLEIKYTPVEDPNLRIAWLLNGKGILASSRIVTFTDFGIAALEINPVNVFDQGEYTVVAVNPLGEARVSANIAVIGHGSFIQQQQSGSQFGGTAYQSKGAQAPAGTHLDL---------PNFHS--DLRSQEVFEGQQIHLETKLTPINDPDLRVVWLLDGNELPSNDKYRQTLSHGFASLDIPQTSSNDSGLYSCRAFNKLGESEN---------QATIIIVPKSD----LQQFEQHRQLDVEDVREIQFAHSSQDLTPKFLSQIQPFHCEQELGRSFFEARIQPINDPTLRVSWLKDGQPLPNANRIQIFQNFGVVSLSLHPTYPEDAGVYTCVLFNSHGQAQS 637 HSP 12 Score: 81.2629 bits (199), Expect = 2.765e-15 Identity = 109/509 (21.41%), Postives = 192/509 (37.72%), Query Frame = 3
Query: 666 AKTIPKFLSQLPNSLTLIQGQPLHIEIF-LEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLTV 2189
+K P +SQ L +QG+ F +E + + W KDG +K R + +L I G+Y+ N+ G E+ + N+T P + + + AR ND + + + PA F I G K + W+ +G+ + R E L + +D G Y V + G + LNV +K+ KG P+ E P F Q++PI ++EG + + D + + WY+N + + +IS +G A L I +A +ED +Y + A+N G+ S A+ +L +G + + ++ G +++Q+++ + P+F K +I+A +G KF +V G P PT++W+ + + K DG LE+ R + + + N G A +A LT+
Sbjct: 3828 SKQAPPTISQ---QLKPLQGELGKAAKFVIEFAGAAPVKVTWLKDGKEIKSTFRTLITTTPTNSSLHIGRLENSHAGEYTVRLENAAGTVESLA-NLTVAPPTTQGKAPDFS-----------------ARLND------------------LRIQQNGPAEFSCQI--GGEPKPTIQWFKDGQPLPNDDRFQVVEEGGAYKLKFSNIISTDAGIYEIVAKNGVGEARCK-ARLNVNLQKT------GKGAE---------EGPRYEA---------PRFTSQIQPI-VVDEGKGAQFSAQFSGFPDPT--IRWYRNNEPVKHADGYEISQSKGEAILRISAARNEDVAEYKVEASNPAGKASS--------------VANLVLTPRSG----RIAKSTISRGGSASYQSSDKAAADS-PHFIAKLSDISARQGHTVKFSAEVDGNPEPTVQWQFKGKPISASNNVKISRDGKRAILELARVTPDSAGEYQIVIRNDKGAATSQAKLTL 4248 HSP 13 Score: 58.151 bits (139), Expect = 3.071e-8 Identity = 86/371 (23.18%), Postives = 133/371 (35.85%), Query Frame = 3
Query: 678 PKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQS-CNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTI----DPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSE-KINIS 1772
P F ++L N L + Q P E + + +WFKDG L R V+ + G L + D G Y VA+N +G+A ++ NV L+ EE RY P F I P I V EG A F DP + WY N E VK + L I D Y +VE+ + +++L P+S I+ + D+ D P FI +L I +G +V + +V+ + V+W I + ++IS D A L + + +G+Y ++ N G S+ K+ +S
Sbjct: 3932 PDFSARL-NDLRIQQNGP--AEFSCQIGGEPKPTIQWFKDGQPLPNDDRFQVVEEGGAYKLKFSNIISTDAGIYEIVAKNGVGEARCKARLNVN--------------------LQKTGKGAEEGPRYE-------APRFTSQIQP--IVVDEGKGAQFSAQFSGFPDP------TIRWYRNNEPVKHADGYEISQSKGEAILRISAARNEDVAEY---------KVEASNPAGKASSVANLVLTPRSGRIAKS---TISRGGSASYQSSDKAAADSPHFIAKLSDIS-ARQGHTVKFSAEVD--GNPEPTVQWQFKGKPISASNNVKISRDGKRAILELARVTPDSAGEYQIVIRNDKGAATSQAKLTLS 4249 HSP 14 Score: 58.151 bits (139), Expect = 3.203e-8 Identity = 57/224 (25.45%), Postives = 93/224 (41.52%), Query Frame = 3
Query: 1530 LNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQ-EECLPNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLE--WRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLTVI 2192
L +GDSV L K A + +W+K+ I +G + TL+I +A ++ G Y ATNK+G T +G +V + A + + + D + + +DV + P F+ + EG A+ +K + P L W ++ ++ S T+ D I LEIN + + V A NPLG A+ A + VI
Sbjct: 178 LYDGDSVKLYCK---AAGEGVSFKWFKDNEPISSGGSYAVDNKGNETTLHINNATMKEGGWYRCDATNKHGTT--------TLKGRVVVNSRQKFNGPAHREMITLRKVDKVERSRTPVNQLQDVSASKSSPKFEGSLQSQQLVEGQSARLEIKYTPVEDPNLRIAWLLNGKGILASSRIVTFTDFGIAALEINPVNVFDQGEYTVVAVNPLGEARVSANIAVI 390
BLAST of Titin vs. Ensembl Fly
Match: sls (gene:FBgn0086906 transcript:FBtr0072848) HSP 1 Score: 264.233 bits (674), Expect = 4.599e-73 Identity = 208/718 (28.97%), Postives = 335/718 (46.66%), Query Frame = 3
Query: 288 ELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEE--DKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQ-LKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQ-SKGIS---AIHALEAELNAPKAE--MKEDEII----TDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLTVIPMVDFRPDLRHITPENPFK----KMAGLKKVECTSELNDAFKKSKPT-AERIVKIEMGSE-TKSKFYKSP 2384
++V+ E R+ P +D S+ ++WF +GK L AGSR TI D GF++L+I + D+G Y C A + G A + +++ G+ QLP +L K ET H EE + PKF ++ ++L + +G P+H + +E D ++ EWF +G + GSR+ + D G+ ALD+ + Y D+G+Y+C A N G A T S VTC + +I+ +S L E S+ LK LE G + PP F I ++V E F+ ++P L +WY NG+ + + R +M +L+ILY D+G Y+C + +G + T++ + +I+L Q +G+ A+ +EA + +E + ED++ P F+ Q++ L E ++ P D ++ VEW+ N +++ +R Q YD G +N EDSG+Y ATN G+ ++ I I IV S L A R++++E AS + + D + P F K T EG PA+F V+V+G P P + W I+ VV S K NDG +L++ + + KV V A N +G + L V+ D D R++ +P ++A +K +EL F + K +E+ G E K K YK P
Sbjct: 3547 DMVIGENALAHFECRLQPINDPSMRVDWFHNGKALWAGSRIKTINDFGFVILEIAGCYQRDSGLYTCKATNKHGEATVS-CKLQVKGRQGIVMEPQLPSNFRTGTESLQKLEET--MHKREELVTEDEQPNPPKFTEEIKDNLDVPEGGPIHFDCRVEPVGDPTMRIEWFYNGHVMATGSRVHQLNDFGFIALDVDYIYARDSGEYTCRATNKWGTA-TTSAKVTCKGKHNIVYESQLPEGMTSEKLKELER-GRIPEAPKVVEEVFGPPKFTTQIT--SVTVDEAEAVRFECQVEPKTDPSLRVEWYRNGKPLPSGHRYRNIFDMGFVSLDILYVYGEDSGEYVCRAINNYGEDRTR-ATVSCKKLPTILLQNQVPRGMKRSDALTQMEATIKKYTSEVHLTEDDLFDPDRKQPPRFVTQIKEQLTLTEMAVTKFECQLAPVGDPNMKVEWFFNGKPLLHKNRFQPIYDFGYVAMNFGWVYPEDSGEYVCRATNLYGKDETRAI-IKVSGKPGIVYDSQLPAHMQSIDRIREME---ASWQVVPDEVDPDAKPRTKPVFVSKLEPQTVEEGDPARFCVRVTGHPRPRVMWLINGHTVVHGSRYKLTNDGMF-HLDVPKTRQYDTGKVEVIARNSVGESIATTELKVVARSD---DYRNVLKNSPRPWYDYELAAYQKERQENELEKVFDERKQVLSEQSSHTLKGVEHLKPKQYKPP 4248 HSP 2 Score: 236.884 bits (603), Expect = 5.086e-64 Identity = 187/692 (27.02%), Postives = 321/692 (46.39%), Query Frame = 3
Query: 258 SPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKA-ETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDP-QSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSA---GYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAG--------EGGPAKFLVKVSGFPTPTLE---WRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLTVIPMVDFRPDLRHITPENPFKKMAGLKKVECTS 2285
+P F + ++ V E + + R+ P D L + W ++G+ + A +R +T+ D G++ L++ Y +D+G Y C A + G A ++ A + + ++ + TQ E M +I+ L+ + Y EE+++ T P F+++L L++GQ H E +E + D +L EWF +G L G R YD G+ ALDI Y ED+G+Y+C N+LG+A + NVT S I E ++++++ LE+ + ++ P F P+ R V+EGAP + + T+ P N + +WY NG ++T R + AL+ILY DTG+Y+C + G + +NV K++ LD ++ + I LE PK ++E P F+ L ++ L EG+ L +VEP ND +L +EW+ N + G R + ++D G L+I+ ED+G Y ATN+ G E +N +C +++ S++ D+ ++++ LE + + + + + P + TA EG A F +V+ P L + L T++ G ++ L+I + V A N LG A L VIP D +H PE + ++K+E T+
Sbjct: 1690 APVFTMPVR-DVRVAENQAVHFEARLIPVGDPKLTVEWLRNGQPIEASNRTTTMHDFGYVALNMKYVNPEDSGTYTCRAVNELGQAVTS-ASLIVQSKTSIQLETQH-EAAMHKIHQLEDH---SRYQRREEEEYTVTTAPVFVTKLIGPSNLVEGQSAHYECRIEPYPDPNLKVEWFHNGKPLSTGHRFRTTYDFGFAALDILTVYAEDSGEYTCRVTNNLGEAINSIVLNVTS---RSSIIHETQHEEALTKIQHLEDTSRFQRKTDEEQFHAERPQFGRPL--RNAKVNEGAPVHLEATLIPVNDPTMKVEWYCNGRPIQTGHRFKTTYDFGFVALDILYAHAEDTGTYMCKAKNAIGEAVTT-CAVNVTANKTLDLDTLDAQRLEKIRQLETYAPPPKPVVEEK---GQKPIFLTPLSNLEHLKEGEHAHLECRVEPINDPNLKIEWFCNGKQLPTGHRYRTTHDFGYVALDILYVYGEDTGTYICKATNQLG----EAVN-TC--NVRVLNRRSMILDTQHPDALEKIQKLESKVPNARTE------------VGDAPISPPHFTAELRGSTEIYEGQTAHFEAQVAPVHDPNLRIEFYHNGKPLPSASRFHITFDFGYVS-LDITHAVAEDAGEYSVRAVNALGQAVSSTNLRVIPRGTIISDTQH--PEG----LEKIRKLESTA 2340 HSP 3 Score: 226.868 bits (577), Expect = 7.745e-61 Identity = 193/664 (29.07%), Postives = 296/664 (44.58%), Query Frame = 3
Query: 261 PTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTI--PKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNL---TESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQ-SKG----ISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQ-LNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQ-TQSEKINISCFEGEKIVTASSLLAD-SAGYQRLKDLEEDLASGNISTFQNTEDVQ-------EECLPNFDVKPMNITAGEGGPAKFLVKVS--GFPTPTLEWRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLT 2186
P F ++ + +G+ + P +D L++ W+ +GKL+ +R T+ D G++VLDI Y + D+G+YVC A + G + + GV Y + P+ + Q I L+ G + D A PKF++Q+ + L++GQ H E L +D LV EW+ +G L G R +D G LDI +CY E++G Y ARN G+ T++ ++ C + S+I S L E + ++ +LE P F P++ E ++ EG A+F+ I P + KL +WY NG +K SR + L I P D+G Y C +E+G + T+ +Q ++SII++ Q KG I I LE L + E D+ P FI P D + E +++P ND S+ V+W+ N + GSR++ D G L I DSG Y ATNK+G+ T S K+ + +G IV L ++ G + L+ LEE + TED Q EE N DV EGGP F +V G PT +EW + ++ S ND L+++ + ATN G A A +T
Sbjct: 3130 PRFTSHIQPLEGLKDGQSAHFECTLIPVNDPDLKVEWYHNGKLMRHSNRIKTVSDFGYVVLDISYLQDHDSGEYVCRAWNKYGEDFTRTT-LNCGGRGGVFYDSLQPDSL-QRIRELE------CPQGQQADTSAPLVAEPPKFITQIVDVTKLVEGQSAHFEARLTPITDPDLVVEWYFNGKKLPHGHRFRTFHDFGIVILDILYCYEENSGVYEARARNKYGEDVTRA-SLKCASKSSLILDSQLPRGMEGGLEKIANLEYSMVRTREETTEETKGKAPVFTVPLENIE-NLREGENAHFEARITPADDPKLKVEWYWNGRPLKAGSRFRTFCDFGFVILEISPVYPEDSGEYSCRAINEYGEAVTT-ATMKIQGKRSIIMESQLPKGMEGTIDRIAELEG-LGSRSTEFVPDDDTGKPPEFITS--PFDMVIGENALAHFECRLQPINDPSMRVDWFHNGKALWAGSRIKTINDFGFVILEIAGCYQRDSGLYTCKATNKHGEATVSCKLQVKGRQG--IVMEPQLPSNFRTGTESLQKLEETMHKREELV---TEDEQPNPPKFTEEIKDNLDVP-------EGGPIHFDCRVEPVGDPTMRIEWFYNGHVMATGSRVHQLNDFGFIALDVDYIYARDSGEYTCRATNKWGTATTSAKVT 3767 HSP 4 Score: 226.483 bits (576), Expect = 1.132e-60 Identity = 181/665 (27.22%), Postives = 314/665 (47.22%), Query Frame = 3
Query: 243 DHLYDSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESS--ISQLKSLENPGEEYARYN--DTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSK-GISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQE--ECLPNFDVKPMN----ITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLTVIPMVD 2204
D +Y P F L++ + EG + + R+ P D ++ + W+ +G+ L+A +R +++F GFI LD+L + D+G+Y+C + SG A+S A + + ++ +Q P + Q IN L+ + Y E P+F+ L + +G+ +H E + +D S+ EW+KDG+ + SRI I++ GY +L+I ED G Y+ A N +G+A +QS ++ H + + E I +++ LE+ + R + + + PP F PI ++ + E A A+F+ ++P + +W +G+ ++ SR+T AL I D G+Y C ++ G+ ++ L V ++ I+ + Q G+ I LE + E +E+ IT P F+ L+ ++ EG +VEP +D L +EWY N I +R+Q YD G L+I +ED+G Y ++A NK G+ Q + I E + SS+ Y++ ++LE N + D++E + P F V P++ I G+ + ++ G PT +EW + V S +TY D L+I + + + V ATN LG A A + VI D
Sbjct: 612 DRVYPPPQFIIPLQN-VQQTEGGRVHMEARIEPVGDPTMVVEWYLNGRPLAASARATSVFKFGFIALDLLSIMGHDSGEYMCRVTNASGVAESR-AILSVVQRPSIEQSSQNPNSL-QYINQLE---DYSRYQRTESIDEQLNQAPQFIRPLRDLGEFEEGKNVHFEAQVTPVNDPSMRVEWYKDGLPITASSRITAIFNFGYVSLNILHLRAEDAGTYTVRAVNRIGEAISQS-SIRVHSRSQVTADLGIPEQQRYIEKVEELEDYRKSQQRRHVQEAAEAIAPPQFKTPIQ-NQLDLREHAHAHFEARLEPVGDSTMRVEWLKDGQPLEASSRITTYHNFGYVALTIKQLTIYDAGTYTCRAYNAMGQ-DTTVAQLTVISKNEIVSESQHPGGLQKIQHLEDSSRYGRRE-EEETYITQAPRFLGPLKGTTKILEGQRAHFEARVEPQSDLGLVIEWYHNGRSITAANRIQTYYDFGYVALDISQVRAEDAGVYLVVARNKLGEAQQQATMI--VETRSSIDTSSM--HRGLYEKTQNLE------NKPFVEPQYDIEEISKSKPVF-VTPLSDPKPIHDGKNIHLECRLEPMGDPTMRVEWFHNGRPVTVGSRFRTYYDFGFVALDIIKATAADSGEYTVRATNHLGTAHTSACVRVIDHTD 1255 HSP 5 Score: 222.246 bits (565), Expect = 2.787e-59 Identity = 171/588 (29.08%), Postives = 275/588 (46.77%), Query Frame = 3
Query: 303 EGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEM---MQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNL---TESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQ-----SKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECL--PNFDVKPMNITAGEGGPAKFLVKVSGFPTPTL--EW 2021
EG+ R+ P D L + W+ +GK L G RF T D G ++LDILY E+++G Y A++ G + A ++ +S + +QLP M +++I NL+ + EE K A P F L N L +G+ H E + D L EW+ +G LK GSR D G+ L+I+ YPED+G+YSC A N G+A T + + + SII +S L E +I ++ LE G + PP FI P ++ + E A A+F+ + P N + DW+HNG+ + SR+ + L I D+G Y C ++HG + L V+ + I+++PQ G ++ LE ++ + + EDE + P F ++++ + EG + +VEP D ++ +EW+ N + + GSR+ D G L++ + DSG+Y ATNK G T + ++C +G+ + S L + ++LK+LE G I V EE P F + ++T E +F +V P+L EW
Sbjct: 3277 EGQSAHFEARLTPITDPDLVVEWYFNGKKLPHGHRFRTFHDFGIVILDILYCYEENSGVYEARARNKYGEDVTR-ASLKCASKSSLILDSQLPRGMEGGLEKIANLEYSMVRTREETTEETKGKA---PVFTVPLENIENLREGENAHFEARITPADDPKLKVEWYWNGRPLKAGSRFRTFCDFGFVILEISPVYPEDSGEYSCRAINEYGEAVT-TATMKIQGKRSIIMESQLPKGMEGTIDRIAELEGLGSRSTEFVPDDDTGKPPEFI--TSPFDMVIGENALAHFECRLQPINDPSMRVDWFHNGKALWAGSRIKTINDFGFVILEIAGCYQRDSGLYTCKATNKHGEA-TVSCKLQVKGRQGIVMEPQLPSNFRTGTESLQKLEETMHKREELVTEDE-QPNPPKFTEEIKDNLDVPEGGPIHFDCRVEPVGDPTMRIEWFYNGHVMATGSRVHQLNDFGFIALDVDYIYARDSGEYTCRATNKWG-TATTSAKVTC-KGKHNIVYESQLPEGMTSEKLKELER----GRIP---EAPKVVEEVFGPPKFTTQITSVTVDEAEAVRFECQVEPKTDPSLRVEW 3846 HSP 6 Score: 217.238 bits (552), Expect = 1.049e-57 Identity = 147/510 (28.82%), Postives = 251/510 (49.22%), Query Frame = 3
Query: 258 SPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSV---IPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQ-SKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISC 1775
+P F LE+ + EGE R+ PADD L++ W+ +G+ L AGSRF T D GF++L+I +D+G+Y C A + G A + A ++I + + +QLP+ M I+ + + G+ P+F++ P + + + H E L+ +D S+ +WF +G +L GSRI I D G+ L+IA CY D+G Y+C A N G+A T SC + + I+ + L + + +SL+ E + + P PP F E I + V EG P +F ++P + +W++NG ++ T SR+ + AL++ Y D+G Y C ++ G + + + + +I+ + Q +G+++ E E K E + P F Q+ + ++E ++V +VEP D SL VEWY+N + +G R + +D G +L+I+ EDSG+Y A N G+ ++ + +SC
Sbjct: 3400 APVFTVPLENIENLREGENAHFEARITPADDPKLKVEWYWNGRPLKAGSRFRTFCDFGFVILEISPVYPEDSGEYSCRAINEYGEAVTT-ATMKIQGKRSIIMESQLPKGMEGTIDRIAELEGLGSRSTEFVPDDDTGKPPEFITS-PFDMVIGENALAHFECRLQPINDPSMRVDWFHNGKALWAGSRIKTINDFGFVILEIAGCYQRDSGLYTCKATNKHGEA-TVSCKLQVKGRQGIVMEPQLPSNFRTGTESLQKLEETMHKREELVTEDEQPNPPKFTEEIK-DNLDVPEGGPIHFDCRVEPVGDPTMRIEWFYNGHVMATGSRVHQLNDFGFIALDVDYIYARDSGEYTCRATNKWGTATTS-AKVTCKGKHNIVYESQLPEGMTSEKLKELERGRIPEAPKVVEEVFGPPKFTTQITSVT-VDEAEAVRFECQVEPKTDPSLRVEWYRNGKPLPSGHRYRNIFDMGFVSLDILYVYGEDSGEYVCRAINNYGEDRT-RATVSC 3902 HSP 7 Score: 210.69 bits (535), Expect = 1.555e-55 Identity = 161/598 (26.92%), Postives = 280/598 (46.82%), Query Frame = 3
Query: 261 PTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPI-DPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQS-KGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADS---AGYQRLKDLEEDLASGNISTFQNTEDVQEECL---PNFDVKPMNIT-AGEGGPAKFLVKVSGFPTPTL--EW 2021
P F+ ++++L + E R+ P D ++ + W KDG+ L A SR +T + G++ L I D G Y C A + G + +A + + ++ + +Q P +Q+I +L+ ++ Y EE++ P+FL L + +++GQ H E +E SD LV EW+ +G S+ +RI YD GY ALDI+ ED G Y VARN LG+A+ Q+ + S I S++ + ++LEN +Y+ + P F+ P+ DP+ I +G + + ++P + +W+HNG V SR + AL+I+ +D+G Y V + H + V ++ + Q+ + + I LE + +ED I P F + L I+ + EG +V L +++P D S+ +EW+ N + G R + +Y+ L+++ + DSG Y A N+ G+ + SC +I+ + L+ ++ +G Q+++ LE+ ST + +E + P F P ++T EGG A F K+ P L EW
Sbjct: 890 PQFKTPIQNQLDLREHAHAHFEARLEPVGDSTMRVEWLKDGQPLEASSRITTYHNFGYVALTIKQLTIYDAGTYTCRAYNAMG-QDTTVAQLTVISKNEIVSESQHPG-GLQKIQHLE---DSSRYGRREEEETYITQAPRFLGPLKGTTKILEGQRAHFEARVEPQSDLGLVIEWYHNGRSITAANRIQTYYDFGYVALDISQVRAEDAGVYLVVARNKLGEAQQQATMIV--ETRSSIDTSSMHRGLYEKTQNLENKPFVEPQYDIEEISKSKPVFVTPLSDPKPI--HDGKNIHLECRLEPMGDPTMRVEWFHNGRPVTVGSRFRTYYDFGFVALDIIKATAADSGEY-TVRATNHLGTAHTSACVRVIDHTDVVTETQNEQSLEQIQLLEDSRR--RHHQEEDITIMQAPQFTRGLHNIETI-EGTNVHLECRLQPVGDPSMRIEWFVNGKPVKTGHRFRPAYEFDYVALDLLGCYAIDSGVYTCQARNQLGEAVT-----SC--SVRIIAKNDLILETQNESGLQKIQYLED-------STRHRRSEFVDEVVNIRPRFLTHPKSLTNTREGGHAHFECKIEPVTDPNLKVEW 1460 HSP 8 Score: 209.534 bits (532), Expect = 3.184e-55 Identity = 173/661 (26.17%), Postives = 298/661 (45.08%), Query Frame = 3
Query: 252 YDSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPE--EMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDP-QSKGISAIHALEAELNAPKAEMKEDE--IITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADS-------AGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNI-TAGEGGPAKFLVKVSGFPTPTL--EWRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLTV 2189
Y P + + L++E + E +P+ L V P +D +L I W+ +GK+L+ GSRF + GF+ +D++ D G Y C A + +G A ++ + + + + TQ P+ E +++I +L+ + G + ++ P+F ++ N + +G+ H E L D S+V EWF +G L+ R+ IY G AL++ ED G Y+C A N G AE SCN+ C ++ P + R+ S I+P++ + +G A+F+ T+ P N L +WYHNG++++ +R+ + L+I Y D+G Y+C ++++G + TLN + D Q + I EL P+ + + ++ + P FI Q+ + +L EG S ++ P D L VEWY N + +G R + +D G L+I+ E+SG Y A NK G+ + + ++ C + SSL+ DS G +++ +LE + + T + T + + P F V NI EG A F +++ P L EW + + S +T+ D LEI+ + A N G A AT+ +
Sbjct: 2889 YPPPVWTKPLQAEFHLSEAQPIHLEANVEPKEDPNLFIEWYFNGKMLNHGSRFKMTSEFGFVTMDMIEVYARDQGIYTCKAYNKAGEAFTSTT-IFCSSKENIIESTQHPKGAEGLEQIQDLEDSLRKD---GSKPEQPDLGIPPRFTTEFVNIADIGEGELAHFEANLIPVGDQSMVIEWFYNGKVLEASHRVRTIYAFGTVALEVLGTKIEDTGTYTCRATNKHGTAEI-SCNLEC----------------------VDKPRGQKPRFT---------SHIQPLE----GLKDGQSAHFECTLIPVNDPDLKVEWYHNGKLMRHSNRIKTVSDFGYVVLDISYLQDHDSGEYVCRAWNKYGE-DFTRTTLNCGGRGGVFYDSLQPDSLQRIR----ELECPQGQQADTSAPLVAEPPKFITQIVDVTKLVEGQSAHFEARLTPITDPDLVVEWYFNGKKLPHGHRFRTFHDFGIVILDILYCYEENSGVYEARARNKYGEDVT-RASLKC------ASKSSLILDSQLPRGMEGGLEKIANLEYSM----VRTREETTEETKGKAPVFTVPLENIENLREGENAHFEARITPADDPKLKVEWYWNGRPLKAGSRFRTFCDFGFVILEISPVYPEDSGEYSCRAINEYGEAVTTATMKI 3493 HSP 9 Score: 208.764 bits (530), Expect = 5.800e-55 Identity = 202/713 (28.33%), Postives = 314/713 (44.04%), Query Frame = 3
Query: 12 HNGAIFKCITRHK--YSFIF-NLDFIAMTTQESMEYNNNSNFNSVPDEGVIPNGTTMAPHALEVVNQV-------NKRSLA-------SPRRGSDH----LYDSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTI-PKFLSQLPNSLT-LIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEM--KEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADS-----------AGYQRLKDLEEDLASGNISTFQNTEDVQEEC--LPNFDVK---PMNITAGEGGPAKFLVKVSGFPTPTL--EW 2021
HNG +R + Y F F LD I T +S EY + N + GT + V++ N++SL S RR + +P F + L + + EG + L R+ P D S+ I WF +GK + G RF ++ ++ LD+L D+G Y C A++ G A ++ + VRI ++ + TQ E +Q+I L E T H E I P+FL+ P SLT +G H E +E +D +L EWFK+G + +G R I+D GY ALDI ED+G Y+C A N +G ETQ + C E I+T + E+ + Q+ LE+ R P F + + + + E A+F+ + P + + +WYHN +K+ SR T AL+I+ TLP D G+Y C ++ G + V + V T+KSI L+ Q + +A+ L+ + K + +DE ++ P F + + ++ E +V ++ P D L VEW +N I +R +D G LN+ EDSG Y A N+ GQ VT++SL+ S A ++ LE+ S +Q E+ + P F K P N+ EG A + ++ +P P L EW
Sbjct: 1196 HNGRPVTVGSRFRTYYDFGFVALDIIKATAADSGEYTVRAT-NHL--------GTAHTSACVRVIDHTDVVTETQNEQSLEQIQLLEDSRRRHHQEEDITIMQAPQFTRGLHN-IETIEGTNVHLECRLQPVGDPSMRIEWFVNGKPVKTGHRFRPAYEFDYVALDLLGCYAIDSGVYTCQARNQLGEAVTSCS-VRIIAKNDLILETQ-NESGLQKIQYL----EDSTRHRRSEFVDEVVNIRPRFLTH-PKSLTNTREGGHAHFECKIEPVTDPNLKVEWFKNGRPITVGHRFRPIHDFGYVALDIVHLIAEDSGVYTCRAVNLIGSDETQ-VELQCRSGEQIVTVTQ-NEAGLEQIHYLEDRSRYTRREEIDESTKQAPVFTTSL--KNVEIKENQRAHFECRLIPVSDPSMRVEWYHNNLPLKSGSRFTETNNFGFVALDIMSTLPEDAGTYTCRAYNAVGEAITSAVAV-VHTKKSIYLESQHE--TALPRLQHLEDGSKRQRISVQDEFVSQAPVFTMPVRDV-RVAENQAVHFEARLIPVGDPKLTVEWLRNGQPIEASNRTTTMHDFGYVALNMKYVNPEDSGTYTCRAVNELGQA---------------VTSASLIVQSKTSIQLETQHEAAMHKIHQLEDH------SRYQRREEEEYTVTTAPVFVTKLIGPSNLV--EGQSAHYECRIEPYPDPNLKVEW 1860 HSP 10 Score: 203.756 bits (517), Expect = 2.609e-53 Identity = 176/656 (26.83%), Postives = 297/656 (45.27%), Query Frame = 3
Query: 168 TMAPHALEVVNQVNKRSLASPRRGSDHLYDSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEM----MQE--INNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKA-ETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQ----SKGISAIHALEAEL-NAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNIT-AGEGGPAKF---LVKVSGFPTPTLEWRID-DVLVVPDSVCKTYNDGSI 2084
T P ++ ++Q+ K LA + L++ P F Q L + EG RV P D SL+ WF +G L GSR T D GF+ LDI + +D G Y+C A + +G A S+ A +++ +S +D +PE ++E +N + + + T + P F + L + L +GQ + +E +E +D +L EWFK+G+SL GSRI +D G L I +D+ Y+C A N +G+A T S + + + +L S+ ++ LE P E R P FI ++ I E F+ ++P +S +W++NG+ ++ ++ + AL++ + ++G Y C + G + TL K++ LD Q G+ A+ E EL N ++ + E P + K L+ L+E + L VEP D +L++EWY N + +GSR +++ + G T++++ + D G Y A NK G+ + I C E I+ ++ + G ++++DLE+ L Q + P F + +NI GEG A F L+ V G + +EW + VL V Y G++
Sbjct: 2458 TQHPDSISRIHQLEK-PLAPRPTEPERLFEKPIFTQLLTGPSELWEGTHAHFEARVVPVGDPSLKFEWFINGVELQMGSRLRTTHDFGFVTLDITAVVPEDAGVYMCRAYNAAGEAVSSTA-MKVKTKSNIDGQPLIPESWEAIRLKEAAMNRVPEMFVDST----------PQQAPVFTTHLQSYDKLHEGQHVLLEAQVEPRADPNLRIEWFKNGISLTTGSRIRSTFDFGLVTLSINGLRADDSAIYTCKATNQVGEAVSTSSLKIE---DRHWLQAESLHPDSLPRIGELEAPKE--GRPEAPEPTYETPVFITHLN--NIECKESDNVRFECNVEPARDPTMSIEWFYNGQPLQAAAKFKSIYDFGYCALDLTNSYAENSGVYTCKATNSKGSATTSG-TLKCTGGKTMFLDTQHPQGEAGLEAVQETEEELANRYTSKTTKPETQYPPPVWTKPLQAEFHLSEAQPIHLEANVEPKEDPNLFIEWYFNGKMLNHGSRFKMTSEFGFVTMDMIEVYARDQGIYTCKAYNKAGEAFT-STTIFCSSKENIIESTQHPKGAEGLEQIQDLEDSLRKDGSKPEQPDLGIP----PRFTTEFVNIADIGEGELAHFEANLIPV-GDQSMVIEWFYNGKVLEASHRVRTIYAFGTV 3087 HSP 11 Score: 202.986 bits (515), Expect = 4.377e-53 Identity = 206/745 (27.65%), Postives = 320/745 (42.95%), Query Frame = 3
Query: 75 FIAMTTQESMEYNNNSNFNSVPDEGVIPNGTTMAPHALEVVNQVNKRSLASPRRGSDHLYDSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPI-DPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQ-SKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQ---TQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAGEGGPAKFLVKV--SGFPTPTLEWRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLTVIPMVDFRPDLRHITPENPFKKMAGLKKVECTSE 2288
+I T + E N N + +I + T P ALE + ++ + + D P F +L + EG+ +V P D +L I ++ +GK L + SRF FD G++ LDI + + +D G+Y A + G A S+ ++R+ P + TQ PE +++I L+ T + E + + P F L N + + Q H E L D +L EW+++ ++ SRI +D G+ +LDI+ ED G Y C A N LG+A T + E+ I SIS++ LE P R + + P F + + P E+ EG A+F+ + P L +W+ NG ++ SRL + L+I +P D G Y+C ++ G S + V+T+ +I P + AI EA +N EM D P F L+ D+L+EG V L +VEP D +L +EW+KN + GSR++ ++D G TL+I ++DS Y ATN+ G+ T S KI E + A SL DS R+ +LE G + T + P F NI E +F V + PT ++EW + + + K+ D L++ ATN G A TL D +H E AGL+ V+ T E
Sbjct: 2162 YICKATNQLGEAVNTCNVRVLNRRSMILD--TQHPDALEKIQKLESKVPNARTEVGDAPISPPHFTAELRGSTEIYEGQTAHFEAQVAPVHDPNLRIEFYHNGKPLPSASRFHITFDFGYVSLDITHAVAEDAGEYSVRAVNALGQAVSS-TNLRVIPRGTIISDTQHPE-GLEKIRKLES---TAPHQRQEPETPGTRQRPVFTQPLQNIDRINEHQTAHFEARLIPVGDPNLKVEWYRNEKIIEDSSRITKQHDFGFVSLDISHIRKEDEGVYMCRAVNPLGEAVTTASMRVV--SEASIQMDTQHPDSISRIHQLEKPLA--PRPTEPERLFEKPIFTQLLTGPSELW--EGTHAHFEARVVPVGDPSLKFEWFINGVELQMGSRLRTTHDFGFVTLDITAVVPEDAGVYMCRAYNAAGEAVSS-TAMKVKTKSNIDGQPLIPESWEAIRLKEAAMNR-VPEMFVDSTPQQAPVFTTHLQSYDKLHEGQHVLLEAQVEPRADPNLRIEWFKNGISLTTGSRIRSTFDFGLVTLSINGLRADDSAIYTCKATNQVGEAVSTSSLKI-----EDRHWLQAESLHPDS--LPRIGELEAP-KEGRPEAPEPTYET-----PVFITHLNNIECKESDNVRFECNVEPARDPTMSIEWFYNGQPLQAAAKFKSIYDFGYCALDLTNSYAENSGVYTCKATNSKGSATTSGTLKCTGGKTMFLDTQHPQGE------AGLEAVQETEE 2872 HSP 12 Score: 197.593 bits (501), Expect = 2.724e-51 Identity = 141/510 (27.65%), Postives = 249/510 (48.82%), Query Frame = 3
Query: 261 PTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQE-INNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTES-----SISQLK-SLENPGEEYARYNDTSVIP---MPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQ----SKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQT 1748
P F ++++ L V EG P+ RV P D ++ I WF +G +++ GSR + D GFI LD+ Y D+G+Y C A + GTA ++ A V + + Y +QLPE M E + L++ +EE PKF +Q+ S+T+ + + + E +E +D SL EW+++G L G R I+D G+ +LDI + Y ED+G+Y C A N+ G+ T++ V+C +I+ ++ + +++Q++ +++ E D P PP F+ I +++++E A F+ + P + +W+ NG+ + +R + A+N + P D+G Y+C + +G+ E+ + + V + I+ D Q + I I +EA E+ D P F+ +LEP + EGD V+V V W N + +++GSR +++ D G L++ D+G +IA N G++
Sbjct: 3676 PKFTEEIKDNLDVPEGGPIHFDCRVEPVGDPTMRIEWFYNGHVMATGSRVHQLNDFGFIALDVDYIYARDSGEYTCRATNKWGTATTS-AKVTCKGKHNIVYESQLPEGMTSEKLKELERGRIPEAPKVVEE----VFGPPKFTTQI-TSVTVDEAEAVRFECQVEPKTDPSLRVEWYRNGKPLPSGHRYRNIFDMGFVSLDILYVYGEDSGEYVCRAINNYGEDRTRA-TVSCKKLPTILLQNQVPRGMKRSDALTQMEATIKKYTSEVHLTEDDLFDPDRKQPPRFVTQIK-EQLTLTEMAVTKFECQLAPVGDPNMKVEWFFNGKPLLHKNRFQPIYDFGYVAMNFGWVYPEDSGEYVCRATNLYGKDETRAI-IKVSGKPGIVYDSQLPAHMQSIDRIREMEASWQVVPDEVDPDAKPRTKPVFVSKLEP-QTVEEGDPARFCVRV--TGHPRPRVMWLINGHTVVHGSRYKLTND-GMFHLDVPKTRQYDTGKVEVIARNSVGES 4172 HSP 13 Score: 186.422 bits (472), Expect = 9.332e-48 Identity = 139/517 (26.89%), Postives = 240/517 (46.42%), Query Frame = 3
Query: 258 SPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYA-RYNDTSVIPM----PPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQ----SKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFE 1781
+P F L+S + EG+ + L +V P D +L I WFK+G L+ GSR + FD G + L I DD+ Y C A + G A S + ++I + + P+ + + I L+ E G E P F++ L N++ + + E +E D ++ EWF +G L+ ++ IYD GYCALD+ Y E++G Y+C A NS G A T S + C +++ + + + L++++ EE A RY + P PP + +P+ E +SE P + + ++P L +WY NG+++ SR E ++++ D G Y C +++ G + T+ ++++II Q ++G+ I LE L ++ ++ ++ P F + I + EG+ + P D S+ +EW+ N + R++ Y GT L ++ ED+G Y ATNK+G T N+ C +
Sbjct: 2621 APVFTTHLQSYDKLHEGQHVLLEAQVEPRADPNLRIEWFKNGISLTTGSRIRSTFDFGLVTLSINGLRADDSAIYTCKATNQVGEAVST-SSLKIEDRHWLQAESLHPDSLPR-IGELEAPKE-----GRPEAPEPTYETPVFITHL-NNIECKESDNVRFECNVEPARDPTMSIEWFYNGQPLQAAAKFKSIYDFGYCALDLTNSYAENSGVYTCKATNSKGSA-TTSGTLKCTGGKTMFLDTQHPQGE-AGLEAVQETEEELANRYTSKTTKPETQYPPPVWTKPLQA-EFHLSEAQPIHLEANVEPKEDPNLFIEWYFNGKMLNHGSRFKMTSEFGFVTMDMIEVYARDQGIYTCKAYNKAGEAFTS-TTIFCSSKENIIESTQHPKGAEGLEQIQDLEDSLRKDGSKPEQPDLGIP-PRFTTEFVNIADIGEGELAHFEANLIPVGDQSMVIEWFYNGKVLEASHRVRTIYAFGTVALEVLGTKIEDTGTYTCRATNKHG-TAEISCNLECVD 3123 HSP 14 Score: 152.525 bits (384), Expect = 4.406e-37 Identity = 119/399 (29.82%), Postives = 189/399 (47.37%), Query Frame = 3
Query: 144 EGVIPNGTTMAPHALEVVNQVNKRSLASPRRGSDHLYDSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEM-----MQEINNLDKNYETGTYHGMEEDKH--AAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQS-CNVTCHPEESIITKSNLTE--SSISQLKSLENPGEEYARYNDTSVIP-MPPSFIEPIDPREISVSEGAPAYFKV-----------------TIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEM 1256
E +P G T E + ++ + + + + ++ P F + S + VDE E +R +V P D SL + W+++GK L +G R+ IFD GF+ LDILY +D+G+YVC A ++ G ++ A V + Q+P M + ++ K Y T H E+D K P+F++Q+ LTL + E L D ++ EWF +G L +R IYD GY A++ + YPED+G+Y C A N GK ET++ V+ P I+ S L SI +++ +E + D P P F+ ++P+ +V EG PA F V T+ G+ KL+ND + ++ KT TGK+E+
Sbjct: 3777 ESQLPEGMTS-----EKLKELERGRIPEAPKVVEEVFGPPKFTTQITS-VTVDEAEAVRFECQVEPKTDPSLRVEWYRNGKPLPSGHRYRNIFDMGFVSLDILYVYGEDSGEYVCRAINNYGEDRTR-ATVSCKKLPTILLQNQVPRGMKRSDALTQMEATIKKY-TSEVHLTEDDLFDPDRKQPPRFVTQIKEQLTLTEMAVTKFECQLAPVGDPNMKVEWFFNGKPLLHKNRFQPIYDFGYVAMNFGWVYPEDSGEYVCRATNLYGKDETRAIIKVSGKP--GIVYDSQLPAHMQSIDRIREMEASWQVVPDEVDPDAKPRTKPVFVSKLEPQ--TVEEGDPARFCVRVTGHPRPRVMWLINGHTVVHGSRYKLTNDGMFHLDVPKTRQYDTGKVEV 4163 HSP 15 Score: 149.828 bits (377), Expect = 3.266e-36 Identity = 169/676 (25.00%), Postives = 289/676 (42.75%), Query Frame = 3
Query: 258 SPTFQQDLESELVVDEGEPLRLMTR-VGPADDGSLEINWFKDG-KLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRIN-PESGVDYLTQ-----LPEEMMQ-------EINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQS-CNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIP----MPPSFIEPIDPREIS-VSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILD----PQSKGISAIHALE-AELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAG--EGGPAKFLVKVSGFPTPTL----EWRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLTV 2189
+P F Q S + V EGEP+ + +G I W KDG ++ S RF I D G L+I +D G Y C A++ +G+ +N A + + P +Y + P ++++ EI L ++ E H ++ P+F+ L N + +G +H+E +E D ++V EW+ +G L +R ++ G+ ALD+ D+G+Y C N+ G AE+++ +V P I +S+ +S+ + LE+ Y+RY T I P FI P+ R++ EG +F+ + P N + +WY +G + SR+T +LNIL+ D G+Y + G S+ ++ V + + D Q + I + LE + + ++E P F ++ L E ++EP D+++ VEW K+ + SR+ ++ G L I D+G Y A N GQ + ++ +IV+ S G Q+++ LE+ G + E+ P F + P+ T EG A F +V P L EW + + + +TY D L+I++ V A N LG A+++AT+ V
Sbjct: 470 APQFVQRF-STMTVREGEPITMSANAIGTPQP---RITWQKDGVQISSTAERFVGI-DGGATCLEIPRVTANDAGWYQCTAQNIAGST-ANRARLYVEVPREQPNYEQRRLNLPRPTKVIEPEPIPGPEIIYL-RHVERAKPHLRPGEEDRVYPPPQFIIPLQN-VQQTEGGRVHMEARIEPVGDPTMVVEWYLNGRPLAASARATSVFKFGFIALDLLSIMGHDSGEYMCRVTNASGVAESRAILSVVQRPS---IEQSSQNPNSLQYINQLED----YSRYQRTESIDEQLNQAPQFIRPL--RDLGEFEEGKNVHFEAQVTPVNDPSMRVEWYKDGLPITASSRITAIFNFGYVSLNILHLRAEDAGTYTVRAVNRIGEAISQS-SIRVHSRSQVTADLGIPEQQRYIEKVEELEDYRKSQQRRHVQEAAEAIAPPQFKTPIQNQLDLREHAHAHFEARLEPVGDSTMRVEWLKDGQPLEASSRITTYHNFGYVALTIKQLTIYDAGTYTCRAYNAMGQ-DTTVAQLTVISKNEIVSESQ---HPGGLQKIQHLEDSSRYGR----REEEETYITQAPRF-LGPLKGTTKILEGQRAHFEARVE--PQSDLGLVIEWYHNGRSITAANRIQTYYDFGYVALDISQVRAEDAGVYLVVARNKLGEAQQQATMIV 1116 HSP 16 Score: 123.635 bits (309), Expect = 4.173e-28 Identity = 133/535 (24.86%), Postives = 223/535 (41.68%), Query Frame = 3
Query: 678 PKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVT-----EWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEES---------------IITKSNLTESSISQLKSLEN------PGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQS-KGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMN-ITAGEGGPAKFLVKVS--GFPTPTLEWRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLTVI 2192
P+F+ + +++T+ +G+P+ + S +++ T W KDG+ + + V D G L+I D G Y C A+N G ++ P E +I + I L+ +E PGEE V P PP FI P+ + + +EG + + I+P + +WY NG + +R T + AL++L + D+G Y+C + + G ES + L+V SI Q+ + I+ LE + E DE + P FI+ L + + EG +V +V P ND S+ VEWYK+ I SR+ ++ G +LNI+ +ED+G Y + A N+ G+ S+ +I ++ + +++++LE+ S Q E + P F N + E A F ++ G T +EW D + S TY++ L I + + A N +G A LTVI
Sbjct: 471 PQFVQRF-STMTVREGEPITM-------SANAIGTPQPRITWQKDGVQISSTAERFVGIDGGATCLEIPRVTANDAGWYQCTAQNIAGSTANRARLYVEVPREQPNYEQRRLNLPRPTKVIEPEPIPGPEIIYLRHVERAKPHLRPGEE------DRVYP-PPQFIIPL--QNVQQTEGGRVHMEARIEPVGDPTMVVEWYLNGRPLAASARATSVFKFGFIALDLLSIMGHDSGEYMCRVTNASGVAESRAI-LSVVQRPSIEQSSQNPNSLQYINQLEDYSRYQRTE-SIDEQLNQAPQFIRPLRDLGEFEEGKNVHFEAQVTPVNDPSMRVEWYKDGLPITASSRITAIFNFGYVSLNILHLRAEDAGTYTVRAVNRIGEAISQS-SIRVHSRSQVTADLGIPEQQRYIEKVEELEDYRKSQQRRHVQ--EAAEAIAPPQFKTPIQNQLDLREHAHAHFEARLEPVGDSTMRVEWLKDGQPLEASSRITTYHNFGYVALTIKQLTIYDAGTYTCRAYNAMGQDTTVAQLTVI 983 HSP 17 Score: 65.4698 bits (158), Expect = 2.527e-10 Identity = 134/681 (19.68%), Postives = 241/681 (35.39%), Query Frame = 3
Query: 363 INWFKDGKLLSAGSRFSTIFDR--GFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYH-------------------------------GMEEDKHAAKTIPK--------FLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYF--KVTIDPG-------NGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQS---------------EKINISCFEGEKIVTASSLLADSAGYQR-LKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAGEGGPAKFLVKVS--GFPTPTLEWRIDDV-LVVPDSVCKTYNDGSINYLEINRCSELG--DIKVHVAATNPLGVAKEEATLTVI 2192
+ W + G L +F ++ G + L I D G+Y C A++ G A I V I PE P +Q I NL+KN + Y G E+D ++ + + +SQ P S LI+G + + L WF +G L + ++ Y G L + D G Y+ +A N G S + P + + QL++ + +PP+F++ REI +EG F +VT +P NG+++ +D H K +G + I+ + L D G+ C+ ++ G V A+EA+LN +++++++ P F+++ + + EG+ +T++ + W K+ I + + + D G L I + D+G Y A N G T + E+ ++ K++ + Y R ++ + L G E+ + P F + N+ EGG ++ G PT +EW ++ L + G ++ ++ S +G + TN GVA+ A L+V+
Sbjct: 118 VTWTRKGAPLLESQKFRMSYNEATGDVSLLINQIGPGDEGEYTCTARNQYGEA---ICSVYIQPEGA-------PMPALQPIQNLEKNIYSNGYSYTSIEEEFRVDTFEYRLLREVSFREAITRRSGYEQDSQLSQELDRNQGPAQAPQISQKPRSSKLIEGSDAVFTARVGSNPKPRLT--WFHNGQRLVASQKYEISYSSGVATLRVKNATARDGGHYTLLAENLQG-CVVSSAVLAVEPAAETAYEPKPVDVMAEQLEAGK---------------ALPPAFVKAFGDREI--TEGRMTRFDCRVTGNPYPEVFWLINGRQVRDDASH-----KILVNESGSHSLMITNVTRL-----DAGAVQCLARNKAGEV----------------------------AIEAQLNV----LEKEQVVA--PQFVQRFSTMT-VREGEPITMSANA--IGTPQPRITWQKDGVQISSTAERFVGIDGGATCLEIPRVTANDAGWYQCTAQNIAGSTANRARLYVEVPREQPNYEQRRLNLPRPTKVIEPEPIPGPEIIYLRHVERAKPHLRPG--------EEDRVYPPPQFIIPLQNVQQTEGGRVHMEARIEPVGDPTMVVEWYLNGRPLAASARATSVFKFG---FIALDLLSIMGHDSGEYMCRVTNASGVAESRAILSVV 710 HSP 18 Score: 60.077 bits (144), Expect = 1.212e-8 Identity = 86/376 (22.87%), Postives = 146/376 (38.83%), Query Frame = 3
Query: 645 MEEDKHAAK---TIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGE-IVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPK--AEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQS 1754
+EE + A K P F++ + S T+ GQ + + L W K+GM ++ + ++ + L I + ED+G Y CVA N+ G+A C+ C I+ S S+ + PG E A P +E + + +V EG+ F+ +D G+ + GE VK + L I P D G+Y CV ++ G ++QT + + P L+AP +K+ + +P+ K T+T KV+ + V+W++ I Q+ +D +A L I + EDSG + + T+ GQ +S
Sbjct: 4483 VEERRRAGKGGIQPPSFVTTI-QSTTVATGQLARFDAKVT--GTRPLDVYWLKNGMKIQPSIKFKMLEEDSVHTLLIIEPFAEDSGRYECVAVNAAGEAR---CDGDC-----IV-------QSPSKPEKPTTPGSEKA-----------PHIVEQLKSQ--TVEEGSKVIFRCRVD---GKPTPTARWMRGENFVKPSRYFQMSRQGEYYQLVISEAFPEDEGTYKCVAENKLG---------SIQTSAQLKVRPIEN-----------LDAPPTITALKDVSVTEGMPAQFK-------------TTVTGKVKATS-----VQWFREGQLIPETPDFQMIFDGNSAVLLIGTTYEEDSGIFTVRVTSSTGQVES 4786
BLAST of Titin vs. Ensembl Fly
Match: sls (gene:FBgn0086906 transcript:FBtr0309042) HSP 1 Score: 263.848 bits (673), Expect = 5.632e-73 Identity = 208/718 (28.97%), Postives = 335/718 (46.66%), Query Frame = 3
Query: 288 ELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEE--DKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQ-LKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQ-SKGIS---AIHALEAELNAPKAE--MKEDEII----TDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLTVIPMVDFRPDLRHITPENPFK----KMAGLKKVECTSELNDAFKKSKPT-AERIVKIEMGSE-TKSKFYKSP 2384
++V+ E R+ P +D S+ ++WF +GK L AGSR TI D GF++L+I + D+G Y C A + G A + +++ G+ QLP +L K ET H EE + PKF ++ ++L + +G P+H + +E D ++ EWF +G + GSR+ + D G+ ALD+ + Y D+G+Y+C A N G A T S VTC + +I+ +S L E S+ LK LE G + PP F I ++V E F+ ++P L +WY NG+ + + R +M +L+ILY D+G Y+C + +G + T++ + +I+L Q +G+ A+ +EA + +E + ED++ P F+ Q++ L E ++ P D ++ VEW+ N +++ +R Q YD G +N EDSG+Y ATN G+ ++ I I IV S L A R++++E AS + + D + P F K T EG PA+F V+V+G P P + W I+ VV S K NDG +L++ + + KV V A N +G + L V+ D D R++ +P ++A +K +EL F + K +E+ G E K K YK P
Sbjct: 3562 DMVIGENALAHFECRLQPINDPSMRVDWFHNGKALWAGSRIKTINDFGFVILEIAGCYQRDSGLYTCKATNKHGEATVS-CKLQVKGRQGIVMEPQLPSNFRTGTESLQKLEET--MHKREELVTEDEQPNPPKFTEEIKDNLDVPEGGPIHFDCRVEPVGDPTMRIEWFYNGHVMATGSRVHQLNDFGFIALDVDYIYARDSGEYTCRATNKWGTA-TTSAKVTCKGKHNIVYESQLPEGMTSEKLKELER-GRIPEAPKVVEEVFGPPKFTTQIT--SVTVDEAEAVRFECQVEPKTDPSLRVEWYRNGKPLPSGHRYRNIFDMGFVSLDILYVYGEDSGEYVCRAINNYGEDRTR-ATVSCKKLPTILLQNQVPRGMKRSDALTQMEATIKKYTSEVHLTEDDLFDPDRKQPPRFVTQIKEQLTLTEMAVTKFECQLAPVGDPNMKVEWFFNGKPLLHKNRFQPIYDFGYVAMNFGWVYPEDSGEYVCRATNLYGKDETRAI-IKVSGKPGIVYDSQLPAHMQSIDRIREME---ASWQVVPDEVDPDAKPRTKPVFVSKLEPQTVEEGDPARFCVRVTGHPRPRVMWLINGHTVVHGSRYKLTNDGMF-HLDVPKTRQYDTGKVEVIARNSVGESIATTELKVVARSD---DYRNVLKNSPRPWYDYELAAYQKERQENELEKVFDERKQVLSEQSSHTLKGVEHLKPKQYKPP 4263 HSP 2 Score: 236.498 bits (602), Expect = 5.623e-64 Identity = 187/692 (27.02%), Postives = 321/692 (46.39%), Query Frame = 3
Query: 258 SPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKA-ETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDP-QSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSA---GYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAG--------EGGPAKFLVKVSGFPTPTLE---WRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLTVIPMVDFRPDLRHITPENPFKKMAGLKKVECTS 2285
+P F + ++ V E + + R+ P D L + W ++G+ + A +R +T+ D G++ L++ Y +D+G Y C A + G A ++ A + + ++ + TQ E M +I+ L+ + Y EE+++ T P F+++L L++GQ H E +E + D +L EWF +G L G R YD G+ ALDI Y ED+G+Y+C N+LG+A + NVT S I E ++++++ LE+ + ++ P F P+ R V+EGAP + + T+ P N + +WY NG ++T R + AL+ILY DTG+Y+C + G + +NV K++ LD ++ + I LE PK ++E P F+ L ++ L EG+ L +VEP ND +L +EW+ N + G R + ++D G L+I+ ED+G Y ATN+ G E +N +C +++ S++ D+ ++++ LE + + + + + P + TA EG A F +V+ P L + L T++ G ++ L+I + V A N LG A L VIP D +H PE + ++K+E T+
Sbjct: 1705 APVFTMPVR-DVRVAENQAVHFEARLIPVGDPKLTVEWLRNGQPIEASNRTTTMHDFGYVALNMKYVNPEDSGTYTCRAVNELGQAVTS-ASLIVQSKTSIQLETQH-EAAMHKIHQLEDH---SRYQRREEEEYTVTTAPVFVTKLIGPSNLVEGQSAHYECRIEPYPDPNLKVEWFHNGKPLSTGHRFRTTYDFGFAALDILTVYAEDSGEYTCRVTNNLGEAINSIVLNVTSR---SSIIHETQHEEALTKIQHLEDTSRFQRKTDEEQFHAERPQFGRPL--RNAKVNEGAPVHLEATLIPVNDPTMKVEWYCNGRPIQTGHRFKTTYDFGFVALDILYAHAEDTGTYMCKAKNAIGEAVTT-CAVNVTANKTLDLDTLDAQRLEKIRQLETYAPPPKPVVEEK---GQKPIFLTPLSNLEHLKEGEHAHLECRVEPINDPNLKIEWFCNGKQLPTGHRYRTTHDFGYVALDILYVYGEDTGTYICKATNQLG----EAVN-TC--NVRVLNRRSMILDTQHPDALEKIQKLESKVPNARTE------------VGDAPISPPHFTAELRGSTEIYEGQTAHFEAQVAPVHDPNLRIEFYHNGKPLPSASRFHITFDFGYVS-LDITHAVAEDAGEYSVRAVNALGQAVSSTNLRVIPRGTIISDTQH--PEG----LEKIRKLESTA 2355 HSP 3 Score: 226.868 bits (577), Expect = 9.285e-61 Identity = 193/664 (29.07%), Postives = 296/664 (44.58%), Query Frame = 3
Query: 261 PTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTI--PKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNL---TESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQ-SKG----ISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQ-LNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQ-TQSEKINISCFEGEKIVTASSLLAD-SAGYQRLKDLEEDLASGNISTFQNTEDVQ-------EECLPNFDVKPMNITAGEGGPAKFLVKVS--GFPTPTLEWRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLT 2186
P F ++ + +G+ + P +D L++ W+ +GKL+ +R T+ D G++VLDI Y + D+G+YVC A + G + + GV Y + P+ + Q I L+ G + D A PKF++Q+ + L++GQ H E L +D LV EW+ +G L G R +D G LDI +CY E++G Y ARN G+ T++ ++ C + S+I S L E + ++ +LE P F P++ E ++ EG A+F+ I P + KL +WY NG +K SR + L I P D+G Y C +E+G + T+ +Q ++SII++ Q KG I I LE L + E D+ P FI P D + E +++P ND S+ V+W+ N + GSR++ D G L I DSG Y ATNK+G+ T S K+ + +G IV L ++ G + L+ LEE + TED Q EE N DV EGGP F +V G PT +EW + ++ S ND L+++ + ATN G A A +T
Sbjct: 3145 PRFTSHIQPLEGLKDGQSAHFECTLIPVNDPDLKVEWYHNGKLMRHSNRIKTVSDFGYVVLDISYLQDHDSGEYVCRAWNKYGEDFTRTT-LNCGGRGGVFYDSLQPDSL-QRIRELE------CPQGQQADTSAPLVAEPPKFITQIVDVTKLVEGQSAHFEARLTPITDPDLVVEWYFNGKKLPHGHRFRTFHDFGIVILDILYCYEENSGVYEARARNKYGEDVTRA-SLKCASKSSLILDSQLPRGMEGGLEKIANLEYSMVRTREETTEETKGKAPVFTVPLENIE-NLREGENAHFEARITPADDPKLKVEWYWNGRPLKAGSRFRTFCDFGFVILEISPVYPEDSGEYSCRAINEYGEAVTT-ATMKIQGKRSIIMESQLPKGMEGTIDRIAELEG-LGSRSTEFVPDDDTGKPPEFITS--PFDMVIGENALAHFECRLQPINDPSMRVDWFHNGKALWAGSRIKTINDFGFVILEIAGCYQRDSGLYTCKATNKHGEATVSCKLQVKGRQG--IVMEPQLPSNFRTGTESLQKLEETMHKREELV---TEDEQPNPPKFTEEIKDNLDVP-------EGGPIHFDCRVEPVGDPTMRIEWFYNGHVMATGSRVHQLNDFGFIALDVDYIYARDSGEYTCRATNKWGTATTSAKVT 3782 HSP 4 Score: 226.483 bits (576), Expect = 1.240e-60 Identity = 181/665 (27.22%), Postives = 314/665 (47.22%), Query Frame = 3
Query: 243 DHLYDSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESS--ISQLKSLENPGEEYARYN--DTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSK-GISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQE--ECLPNFDVKPMN----ITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLTVIPMVD 2204
D +Y P F L++ + EG + + R+ P D ++ + W+ +G+ L+A +R +++F GFI LD+L + D+G+Y+C + SG A+S A + + ++ +Q P + Q IN L+ + Y E P+F+ L + +G+ +H E + +D S+ EW+KDG+ + SRI I++ GY +L+I ED G Y+ A N +G+A +QS ++ H + + E I +++ LE+ + R + + + PP F PI ++ + E A A+F+ ++P + +W +G+ ++ SR+T AL I D G+Y C ++ G+ ++ L V ++ I+ + Q G+ I LE + E +E+ IT P F+ L+ ++ EG +VEP +D L +EWY N I +R+Q YD G L+I +ED+G Y ++A NK G+ Q + I E + SS+ Y++ ++LE N + D++E + P F V P++ I G+ + ++ G PT +EW + V S +TY D L+I + + + V ATN LG A A + VI D
Sbjct: 627 DRVYPPPQFIIPLQN-VQQTEGGRVHMEARIEPVGDPTMVVEWYLNGRPLAASARATSVFKFGFIALDLLSIMGHDSGEYMCRVTNASGVAESR-AILSVVQRPSIEQSSQNPNSL-QYINQLE---DYSRYQRTESIDEQLNQAPQFIRPLRDLGEFEEGKNVHFEAQVTPVNDPSMRVEWYKDGLPITASSRITAIFNFGYVSLNILHLRAEDAGTYTVRAVNRIGEAISQS-SIRVHSRSQVTADLGIPEQQRYIEKVEELEDYRKSQQRRHVQEAAEAIAPPQFKTPIQ-NQLDLREHAHAHFEARLEPVGDSTMRVEWLKDGQPLEASSRITTYHNFGYVALTIKQLTIYDAGTYTCRAYNAMGQ-DTTVAQLTVISKNEIVSESQHPGGLQKIQHLEDSSRYGRRE-EEETYITQAPRFLGPLKGTTKILEGQRAHFEARVEPQSDLGLVIEWYHNGRSITAANRIQTYYDFGYVALDISQVRAEDAGVYLVVARNKLGEAQQQATMI--VETRSSIDTSSM--HRGLYEKTQNLE------NKPFVEPQYDIEEISKSKPVF-VTPLSDPKPIHDGKNIHLECRLEPMGDPTMRVEWFHNGRPVTVGSRFRTYYDFGFVALDIIKATAADSGEYTVRATNHLGTAHTSACVRVIDHTD 1270 HSP 5 Score: 222.246 bits (565), Expect = 3.052e-59 Identity = 171/588 (29.08%), Postives = 275/588 (46.77%), Query Frame = 3
Query: 303 EGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEM---MQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNL---TESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQ-----SKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECL--PNFDVKPMNITAGEGGPAKFLVKVSGFPTPTL--EW 2021
EG+ R+ P D L + W+ +GK L G RF T D G ++LDILY E+++G Y A++ G + A ++ +S + +QLP M +++I NL+ + EE K A P F L N L +G+ H E + D L EW+ +G LK GSR D G+ L+I+ YPED+G+YSC A N G+A T + + + SII +S L E +I ++ LE G + PP FI P ++ + E A A+F+ + P N + DW+HNG+ + SR+ + L I D+G Y C ++HG + L V+ + I+++PQ G ++ LE ++ + + EDE + P F ++++ + EG + +VEP D ++ +EW+ N + + GSR+ D G L++ + DSG+Y ATNK G T + ++C +G+ + S L + ++LK+LE G I V EE P F + ++T E +F +V P+L EW
Sbjct: 3292 EGQSAHFEARLTPITDPDLVVEWYFNGKKLPHGHRFRTFHDFGIVILDILYCYEENSGVYEARARNKYGEDVTR-ASLKCASKSSLILDSQLPRGMEGGLEKIANLEYSMVRTREETTEETKGKA---PVFTVPLENIENLREGENAHFEARITPADDPKLKVEWYWNGRPLKAGSRFRTFCDFGFVILEISPVYPEDSGEYSCRAINEYGEAVT-TATMKIQGKRSIIMESQLPKGMEGTIDRIAELEGLGSRSTEFVPDDDTGKPPEFI--TSPFDMVIGENALAHFECRLQPINDPSMRVDWFHNGKALWAGSRIKTINDFGFVILEIAGCYQRDSGLYTCKATNKHGEA-TVSCKLQVKGRQGIVMEPQLPSNFRTGTESLQKLEETMHKREELVTEDE-QPNPPKFTEEIKDNLDVPEGGPIHFDCRVEPVGDPTMRIEWFYNGHVMATGSRVHQLNDFGFIALDVDYIYARDSGEYTCRATNKWG-TATTSAKVTC-KGKHNIVYESQLPEGMTSEKLKELER----GRIP---EAPKVVEEVFGPPKFTTQITSVTVDEAEAVRFECQVEPKTDPSLRVEW 3861 HSP 6 Score: 217.238 bits (552), Expect = 1.257e-57 Identity = 147/510 (28.82%), Postives = 251/510 (49.22%), Query Frame = 3
Query: 258 SPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSV---IPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQ-SKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISC 1775
+P F LE+ + EGE R+ PADD L++ W+ +G+ L AGSRF T D GF++L+I +D+G+Y C A + G A + A ++I + + +QLP+ M I+ + + G+ P+F++ P + + + H E L+ +D S+ +WF +G +L GSRI I D G+ L+IA CY D+G Y+C A N G+A T SC + + I+ + L + + +SL+ E + + P PP F E I + V EG P +F ++P + +W++NG ++ T SR+ + AL++ Y D+G Y C ++ G + + + + +I+ + Q +G+++ E E K E + P F Q+ + ++E ++V +VEP D SL VEWY+N + +G R + +D G +L+I+ EDSG+Y A N G+ ++ + +SC
Sbjct: 3415 APVFTVPLENIENLREGENAHFEARITPADDPKLKVEWYWNGRPLKAGSRFRTFCDFGFVILEISPVYPEDSGEYSCRAINEYGEAVTT-ATMKIQGKRSIIMESQLPKGMEGTIDRIAELEGLGSRSTEFVPDDDTGKPPEFITS-PFDMVIGENALAHFECRLQPINDPSMRVDWFHNGKALWAGSRIKTINDFGFVILEIAGCYQRDSGLYTCKATNKHGEA-TVSCKLQVKGRQGIVMEPQLPSNFRTGTESLQKLEETMHKREELVTEDEQPNPPKFTEEIK-DNLDVPEGGPIHFDCRVEPVGDPTMRIEWFYNGHVMATGSRVHQLNDFGFIALDVDYIYARDSGEYTCRATNKWGTATTS-AKVTCKGKHNIVYESQLPEGMTSEKLKELERGRIPEAPKVVEEVFGPPKFTTQITSVT-VDEAEAVRFECQVEPKTDPSLRVEWYRNGKPLPSGHRYRNIFDMGFVSLDILYVYGEDSGEYVCRAINNYGEDRT-RATVSC 3917 HSP 7 Score: 210.69 bits (535), Expect = 1.717e-55 Identity = 161/598 (26.92%), Postives = 280/598 (46.82%), Query Frame = 3
Query: 261 PTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPI-DPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQS-KGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADS---AGYQRLKDLEEDLASGNISTFQNTEDVQEECL---PNFDVKPMNIT-AGEGGPAKFLVKVSGFPTPTL--EW 2021
P F+ ++++L + E R+ P D ++ + W KDG+ L A SR +T + G++ L I D G Y C A + G + +A + + ++ + +Q P +Q+I +L+ ++ Y EE++ P+FL L + +++GQ H E +E SD LV EW+ +G S+ +RI YD GY ALDI+ ED G Y VARN LG+A+ Q+ + S I S++ + ++LEN +Y+ + P F+ P+ DP+ I +G + + ++P + +W+HNG V SR + AL+I+ +D+G Y V + H + V ++ + Q+ + + I LE + +ED I P F + L I+ + EG +V L +++P D S+ +EW+ N + G R + +Y+ L+++ + DSG Y A N+ G+ + SC +I+ + L+ ++ +G Q+++ LE+ ST + +E + P F P ++T EGG A F K+ P L EW
Sbjct: 905 PQFKTPIQNQLDLREHAHAHFEARLEPVGDSTMRVEWLKDGQPLEASSRITTYHNFGYVALTIKQLTIYDAGTYTCRAYNAMG-QDTTVAQLTVISKNEIVSESQHPG-GLQKIQHLE---DSSRYGRREEEETYITQAPRFLGPLKGTTKILEGQRAHFEARVEPQSDLGLVIEWYHNGRSITAANRIQTYYDFGYVALDISQVRAEDAGVYLVVARNKLGEAQQQATMIV--ETRSSIDTSSMHRGLYEKTQNLENKPFVEPQYDIEEISKSKPVFVTPLSDPKPI--HDGKNIHLECRLEPMGDPTMRVEWFHNGRPVTVGSRFRTYYDFGFVALDIIKATAADSGEY-TVRATNHLGTAHTSACVRVIDHTDVVTETQNEQSLEQIQLLEDSRR--RHHQEEDITIMQAPQFTRGLHNIETI-EGTNVHLECRLQPVGDPSMRIEWFVNGKPVKTGHRFRPAYEFDYVALDLLGCYAIDSGVYTCQARNQLGEAVT-----SC--SVRIIAKNDLILETQNESGLQKIQYLED-------STRHRRSEFVDEVVNIRPRFLTHPKSLTNTREGGHAHFECKIEPVTDPNLKVEW 1475 HSP 8 Score: 209.534 bits (532), Expect = 3.811e-55 Identity = 173/661 (26.17%), Postives = 298/661 (45.08%), Query Frame = 3
Query: 252 YDSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPE--EMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDP-QSKGISAIHALEAELNAPKAEMKEDE--IITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADS-------AGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNI-TAGEGGPAKFLVKVSGFPTPTL--EWRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLTV 2189
Y P + + L++E + E +P+ L V P +D +L I W+ +GK+L+ GSRF + GF+ +D++ D G Y C A + +G A ++ + + + + TQ P+ E +++I +L+ + G + ++ P+F ++ N + +G+ H E L D S+V EWF +G L+ R+ IY G AL++ ED G Y+C A N G AE SCN+ C ++ P + R+ S I+P++ + +G A+F+ T+ P N L +WYHNG++++ +R+ + L+I Y D+G Y+C ++++G + TLN + D Q + I EL P+ + + ++ + P FI Q+ + +L EG S ++ P D L VEWY N + +G R + +D G L+I+ E+SG Y A NK G+ + + ++ C + SSL+ DS G +++ +LE + + T + T + + P F V NI EG A F +++ P L EW + + S +T+ D LEI+ + A N G A AT+ +
Sbjct: 2904 YPPPVWTKPLQAEFHLSEAQPIHLEANVEPKEDPNLFIEWYFNGKMLNHGSRFKMTSEFGFVTMDMIEVYARDQGIYTCKAYNKAGEAFTSTT-IFCSSKENIIESTQHPKGAEGLEQIQDLEDSLRKD---GSKPEQPDLGIPPRFTTEFVNIADIGEGELAHFEANLIPVGDQSMVIEWFYNGKVLEASHRVRTIYAFGTVALEVLGTKIEDTGTYTCRATNKHGTAEI-SCNLEC----------------------VDKPRGQKPRFT---------SHIQPLE----GLKDGQSAHFECTLIPVNDPDLKVEWYHNGKLMRHSNRIKTVSDFGYVVLDISYLQDHDSGEYVCRAWNKYGE-DFTRTTLNCGGRGGVFYDSLQPDSLQRIR----ELECPQGQQADTSAPLVAEPPKFITQIVDVTKLVEGQSAHFEARLTPITDPDLVVEWYFNGKKLPHGHRFRTFHDFGIVILDILYCYEENSGVYEARARNKYGEDVT-RASLKC------ASKSSLILDSQLPRGMEGGLEKIANLEYSM----VRTREETTEETKGKAPVFTVPLENIENLREGENAHFEARITPADDPKLKVEWYWNGRPLKAGSRFRTFCDFGFVILEISPVYPEDSGEYSCRAINEYGEAVTTATMKI 3508 HSP 9 Score: 208.764 bits (530), Expect = 7.129e-55 Identity = 200/708 (28.25%), Postives = 312/708 (44.07%), Query Frame = 3
Query: 12 HNGAIFKCITRHK--YSFIF-NLDFIAMTTQESMEYNNNSNFNSVPDEGVIPNGTTMAPHALEVVNQV-------NKRSLA-------SPRRGSDH----LYDSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTI-PKFLSQLPNSLT-LIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEM--KEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEE--------CLPNFDVK---PMNITAGEGGPAKFLVKVSGFPTPTL--EW 2021
HNG +R + Y F F LD I T +S EY + N + GT + V++ N++SL S RR + +P F + L + + EG + L R+ P D S+ I WF +GK + G RF ++ ++ LD+L D+G Y C A++ G A ++ + VRI ++ + TQ E +Q+I L E T H E I P+FL+ P SLT +G H E +E +D +L EWFK+G + +G R I+D GY ALDI ED+G Y+C A N +G ETQ + C E I+T + E+ + Q+ LE+ R P F + + + + E A+F+ + P + + +WYHN +K+ SR T AL+I+ TLP D G+Y C ++ G + V + V T+KSI L+ Q + +A+ L+ + K + +DE ++ P F + + ++ E +V ++ P D L VEW +N I +R +D G LN+ EDSG Y A N+ GQ VT++SL+ S +L + + + A I ++ Q P F K P N+ EG A + ++ +P P L EW
Sbjct: 1211 HNGRPVTVGSRFRTYYDFGFVALDIIKATAADSGEYTVRAT-NHL--------GTAHTSACVRVIDHTDVVTETQNEQSLEQIQLLEDSRRRHHQEEDITIMQAPQFTRGLHN-IETIEGTNVHLECRLQPVGDPSMRIEWFVNGKPVKTGHRFRPAYEFDYVALDLLGCYAIDSGVYTCQARNQLGEAVTSCS-VRIIAKNDLILETQ-NESGLQKIQYL----EDSTRHRRSEFVDEVVNIRPRFLTH-PKSLTNTREGGHAHFECKIEPVTDPNLKVEWFKNGRPITVGHRFRPIHDFGYVALDIVHLIAEDSGVYTCRAVNLIGSDETQ-VELQCRSGEQIVTVTQ-NEAGLEQIHYLEDRSRYTRREEIDESTKQAPVFTTSL--KNVEIKENQRAHFECRLIPVSDPSMRVEWYHNNLPLKSGSRFTETNNFGFVALDIMSTLPEDAGTYTCRAYNAVGEAITSAVAV-VHTKKSIYLESQHE--TALPRLQHLEDGSKRQRISVQDEFVSQAPVFTMPVRDV-RVAENQAVHFEARLIPVGDPKLTVEWLRNGQPIEASNRTTTMHDFGYVALNMKYVNPEDSGTYTCRAVNELGQA---------------VTSASLIVQSKTSIQL-ETQHEAAMHKIHQLEDHSRYQRREEEEYTVTTAPVFVTKLIGPSNLV--EGQSAHYECRIEPYPDPNLKVEW 1875 HSP 10 Score: 203.756 bits (517), Expect = 3.011e-53 Identity = 176/656 (26.83%), Postives = 297/656 (45.27%), Query Frame = 3
Query: 168 TMAPHALEVVNQVNKRSLASPRRGSDHLYDSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEM----MQE--INNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKA-ETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQ----SKGISAIHALEAEL-NAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNIT-AGEGGPAKF---LVKVSGFPTPTLEWRID-DVLVVPDSVCKTYNDGSI 2084
T P ++ ++Q+ K LA + L++ P F Q L + EG RV P D SL+ WF +G L GSR T D GF+ LDI + +D G Y+C A + +G A S+ A +++ +S +D +PE ++E +N + + + T + P F + L + L +GQ + +E +E +D +L EWFK+G+SL GSRI +D G L I +D+ Y+C A N +G+A T S + + + +L S+ ++ LE P E R P FI ++ I E F+ ++P +S +W++NG+ ++ ++ + AL++ + ++G Y C + G + TL K++ LD Q G+ A+ E EL N ++ + E P + K L+ L+E + L VEP D +L++EWY N + +GSR +++ + G T++++ + D G Y A NK G+ + I C E I+ ++ + G ++++DLE+ L Q + P F + +NI GEG A F L+ V G + +EW + VL V Y G++
Sbjct: 2473 TQHPDSISRIHQLEK-PLAPRPTEPERLFEKPIFTQLLTGPSELWEGTHAHFEARVVPVGDPSLKFEWFINGVELQMGSRLRTTHDFGFVTLDITAVVPEDAGVYMCRAYNAAGEAVSSTA-MKVKTKSNIDGQPLIPESWEAIRLKEAAMNRVPEMFVDST----------PQQAPVFTTHLQSYDKLHEGQHVLLEAQVEPRADPNLRIEWFKNGISLTTGSRIRSTFDFGLVTLSINGLRADDSAIYTCKATNQVGEAVSTSSLKIE---DRHWLQAESLHPDSLPRIGELEAPKE--GRPEAPEPTYETPVFITHLN--NIECKESDNVRFECNVEPARDPTMSIEWFYNGQPLQAAAKFKSIYDFGYCALDLTNSYAENSGVYTCKATNSKGSATTSG-TLKCTGGKTMFLDTQHPQGEAGLEAVQETEEELANRYTSKTTKPETQYPPPVWTKPLQAEFHLSEAQPIHLEANVEPKEDPNLFIEWYFNGKMLNHGSRFKMTSEFGFVTMDMIEVYARDQGIYTCKAYNKAGEAFT-STTIFCSSKENIIESTQHPKGAEGLEQIQDLEDSLRKDGSKPEQPDLGIP----PRFTTEFVNIADIGEGELAHFEANLIPV-GDQSMVIEWFYNGKVLEASHRVRTIYAFGTV 3102 HSP 11 Score: 202.986 bits (515), Expect = 5.006e-53 Identity = 206/745 (27.65%), Postives = 320/745 (42.95%), Query Frame = 3
Query: 75 FIAMTTQESMEYNNNSNFNSVPDEGVIPNGTTMAPHALEVVNQVNKRSLASPRRGSDHLYDSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPI-DPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQ-SKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQ---TQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAGEGGPAKFLVKV--SGFPTPTLEWRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLTVIPMVDFRPDLRHITPENPFKKMAGLKKVECTSE 2288
+I T + E N N + +I + T P ALE + ++ + + D P F +L + EG+ +V P D +L I ++ +GK L + SRF FD G++ LDI + + +D G+Y A + G A S+ ++R+ P + TQ PE +++I L+ T + E + + P F L N + + Q H E L D +L EW+++ ++ SRI +D G+ +LDI+ ED G Y C A N LG+A T + E+ I SIS++ LE P R + + P F + + P E+ EG A+F+ + P L +W+ NG ++ SRL + L+I +P D G Y+C ++ G S + V+T+ +I P + AI EA +N EM D P F L+ D+L+EG V L +VEP D +L +EW+KN + GSR++ ++D G TL+I ++DS Y ATN+ G+ T S KI E + A SL DS R+ +LE G + T + P F NI E +F V + PT ++EW + + + K+ D L++ ATN G A TL D +H E AGL+ V+ T E
Sbjct: 2177 YICKATNQLGEAVNTCNVRVLNRRSMILD--TQHPDALEKIQKLESKVPNARTEVGDAPISPPHFTAELRGSTEIYEGQTAHFEAQVAPVHDPNLRIEFYHNGKPLPSASRFHITFDFGYVSLDITHAVAEDAGEYSVRAVNALGQAVSS-TNLRVIPRGTIISDTQHPE-GLEKIRKLES---TAPHQRQEPETPGTRQRPVFTQPLQNIDRINEHQTAHFEARLIPVGDPNLKVEWYRNEKIIEDSSRITKQHDFGFVSLDISHIRKEDEGVYMCRAVNPLGEAVTTASMRVV--SEASIQMDTQHPDSISRIHQLEKPLA--PRPTEPERLFEKPIFTQLLTGPSELW--EGTHAHFEARVVPVGDPSLKFEWFINGVELQMGSRLRTTHDFGFVTLDITAVVPEDAGVYMCRAYNAAGEAVSS-TAMKVKTKSNIDGQPLIPESWEAIRLKEAAMNR-VPEMFVDSTPQQAPVFTTHLQSYDKLHEGQHVLLEAQVEPRADPNLRIEWFKNGISLTTGSRIRSTFDFGLVTLSINGLRADDSAIYTCKATNQVGEAVSTSSLKI-----EDRHWLQAESLHPDS--LPRIGELEAP-KEGRPEAPEPTYET-----PVFITHLNNIECKESDNVRFECNVEPARDPTMSIEWFYNGQPLQAAAKFKSIYDFGYCALDLTNSYAENSGVYTCKATNSKGSATTSGTLKCTGGKTMFLDTQHPQGE------AGLEAVQETEE 2887 HSP 12 Score: 197.208 bits (500), Expect = 3.170e-51 Identity = 141/510 (27.65%), Postives = 249/510 (48.82%), Query Frame = 3
Query: 261 PTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQE-INNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTES-----SISQLK-SLENPGEEYARYNDTSVIP---MPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQ----SKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQT 1748
P F ++++ L V EG P+ RV P D ++ I WF +G +++ GSR + D GFI LD+ Y D+G+Y C A + GTA ++ A V + + Y +QLPE M E + L++ +EE PKF +Q+ S+T+ + + + E +E +D SL EW+++G L G R I+D G+ +LDI + Y ED+G+Y C A N+ G+ T++ V+C +I+ ++ + +++Q++ +++ E D P PP F+ I +++++E A F+ + P + +W+ NG+ + +R + A+N + P D+G Y+C + +G+ E+ + + V + I+ D Q + I I +EA E+ D P F+ +LEP + EGD V+V V W N + +++GSR +++ D G L++ D+G +IA N G++
Sbjct: 3691 PKFTEEIKDNLDVPEGGPIHFDCRVEPVGDPTMRIEWFYNGHVMATGSRVHQLNDFGFIALDVDYIYARDSGEYTCRATNKWGTATTS-AKVTCKGKHNIVYESQLPEGMTSEKLKELERGRIPEAPKVVEE----VFGPPKFTTQI-TSVTVDEAEAVRFECQVEPKTDPSLRVEWYRNGKPLPSGHRYRNIFDMGFVSLDILYVYGEDSGEYVCRAINNYGEDRTRA-TVSCKKLPTILLQNQVPRGMKRSDALTQMEATIKKYTSEVHLTEDDLFDPDRKQPPRFVTQIK-EQLTLTEMAVTKFECQLAPVGDPNMKVEWFFNGKPLLHKNRFQPIYDFGYVAMNFGWVYPEDSGEYVCRATNLYGKDETRAI-IKVSGKPGIVYDSQLPAHMQSIDRIREMEASWQVVPDEVDPDAKPRTKPVFVSKLEP-QTVEEGDPARFCVRV--TGHPRPRVMWLINGHTVVHGSRYKLTND-GMFHLDVPKTRQYDTGKVEVIARNSVGES 4187 HSP 13 Score: 186.422 bits (472), Expect = 1.085e-47 Identity = 139/517 (26.89%), Postives = 240/517 (46.42%), Query Frame = 3
Query: 258 SPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYA-RYNDTSVIPM----PPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQ----SKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFE 1781
+P F L+S + EG+ + L +V P D +L I WFK+G L+ GSR + FD G + L I DD+ Y C A + G A S + ++I + + P+ + + I L+ E G E P F++ L N++ + + E +E D ++ EWF +G L+ ++ IYD GYCALD+ Y E++G Y+C A NS G A T S + C +++ + + + L++++ EE A RY + P PP + +P+ E +SE P + + ++P L +WY NG+++ SR E ++++ D G Y C +++ G + T+ ++++II Q ++G+ I LE L ++ ++ ++ P F + I + EG+ + P D S+ +EW+ N + R++ Y GT L ++ ED+G Y ATNK+G T N+ C +
Sbjct: 2636 APVFTTHLQSYDKLHEGQHVLLEAQVEPRADPNLRIEWFKNGISLTTGSRIRSTFDFGLVTLSINGLRADDSAIYTCKATNQVGEAVST-SSLKIEDRHWLQAESLHPDSLPR-IGELEAPKE-----GRPEAPEPTYETPVFITHL-NNIECKESDNVRFECNVEPARDPTMSIEWFYNGQPLQAAAKFKSIYDFGYCALDLTNSYAENSGVYTCKATNSKGSA-TTSGTLKCTGGKTMFLDTQHPQGE-AGLEAVQETEEELANRYTSKTTKPETQYPPPVWTKPLQA-EFHLSEAQPIHLEANVEPKEDPNLFIEWYFNGKMLNHGSRFKMTSEFGFVTMDMIEVYARDQGIYTCKAYNKAGEAFTS-TTIFCSSKENIIESTQHPKGAEGLEQIQDLEDSLRKDGSKPEQPDLGIP-PRFTTEFVNIADIGEGELAHFEANLIPVGDQSMVIEWFYNGKVLEASHRVRTIYAFGTVALEVLGTKIEDTGTYTCRATNKHG-TAEISCNLECVD 3138 HSP 14 Score: 152.14 bits (383), Expect = 5.285e-37 Identity = 119/399 (29.82%), Postives = 189/399 (47.37%), Query Frame = 3
Query: 144 EGVIPNGTTMAPHALEVVNQVNKRSLASPRRGSDHLYDSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEM-----MQEINNLDKNYETGTYHGMEEDKH--AAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQS-CNVTCHPEESIITKSNLTE--SSISQLKSLENPGEEYARYNDTSVIP-MPPSFIEPIDPREISVSEGAPAYFKV-----------------TIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEM 1256
E +P G T E + ++ + + + + ++ P F + S + VDE E +R +V P D SL + W+++GK L +G R+ IFD GF+ LDILY +D+G+YVC A ++ G ++ A V + Q+P M + ++ K Y T H E+D K P+F++Q+ LTL + E L D ++ EWF +G L +R IYD GY A++ + YPED+G+Y C A N GK ET++ V+ P I+ S L SI +++ +E + D P P F+ ++P+ +V EG PA F V T+ G+ KL+ND + ++ KT TGK+E+
Sbjct: 3792 ESQLPEGMTS-----EKLKELERGRIPEAPKVVEEVFGPPKFTTQITS-VTVDEAEAVRFECQVEPKTDPSLRVEWYRNGKPLPSGHRYRNIFDMGFVSLDILYVYGEDSGEYVCRAINNYGEDRTR-ATVSCKKLPTILLQNQVPRGMKRSDALTQMEATIKKY-TSEVHLTEDDLFDPDRKQPPRFVTQIKEQLTLTEMAVTKFECQLAPVGDPNMKVEWFFNGKPLLHKNRFQPIYDFGYVAMNFGWVYPEDSGEYVCRATNLYGKDETRAIIKVSGKP--GIVYDSQLPAHMQSIDRIREMEASWQVVPDEVDPDAKPRTKPVFVSKLEPQ--TVEEGDPARFCVRVTGHPRPRVMWLINGHTVVHGSRYKLTNDGMFHLDVPKTRQYDTGKVEV 4178 HSP 15 Score: 149.443 bits (376), Expect = 3.624e-36 Identity = 169/676 (25.00%), Postives = 289/676 (42.75%), Query Frame = 3
Query: 258 SPTFQQDLESELVVDEGEPLRL-MTRVGPADDGSLEINWFKDG-KLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRIN-PESGVDYLTQ-----LPEEMMQ-------EINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQS-CNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIP----MPPSFIEPIDPREIS-VSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILD----PQSKGISAIHALE-AELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAG--EGGPAKFLVKVSGFPTPTL----EWRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLTV 2189
+P F Q S + V EGEP+ + +G I W KDG ++ S RF I D G L+I +D G Y C A++ +G+ +N A + + P +Y + P ++++ EI L ++ E H ++ P+F+ L N + +G +H+E +E D ++V EW+ +G L +R ++ G+ ALD+ D+G+Y C N+ G AE+++ +V P I +S+ +S+ + LE+ Y+RY T I P FI P+ R++ EG +F+ + P N + +WY +G + SR+T +LNIL+ D G+Y + G S+ ++ V + + D Q + I + LE + + ++E P F ++ L E ++EP D+++ VEW K+ + SR+ ++ G L I D+G Y A N GQ + ++ +IV+ S G Q+++ LE+ G + E+ P F + P+ T EG A F +V P L EW + + + +TY D L+I++ V A N LG A+++AT+ V
Sbjct: 485 APQFVQRF-STMTVREGEPITMSANAIGTPQP---RITWQKDGVQISSTAERFVGI-DGGATCLEIPRVTANDAGWYQCTAQNIAGST-ANRARLYVEVPREQPNYEQRRLNLPRPTKVIEPEPIPGPEIIYL-RHVERAKPHLRPGEEDRVYPPPQFIIPLQN-VQQTEGGRVHMEARIEPVGDPTMVVEWYLNGRPLAASARATSVFKFGFIALDLLSIMGHDSGEYMCRVTNASGVAESRAILSVVQRPS---IEQSSQNPNSLQYINQLED----YSRYQRTESIDEQLNQAPQFIRPL--RDLGEFEEGKNVHFEAQVTPVNDPSMRVEWYKDGLPITASSRITAIFNFGYVSLNILHLRAEDAGTYTVRAVNRIGEAISQS-SIRVHSRSQVTADLGIPEQQRYIEKVEELEDYRKSQQRRHVQEAAEAIAPPQFKTPIQNQLDLREHAHAHFEARLEPVGDSTMRVEWLKDGQPLEASSRITTYHNFGYVALTIKQLTIYDAGTYTCRAYNAMGQ-DTTVAQLTVISKNEIVSESQ---HPGGLQKIQHLEDSSRYGR----REEEETYITQAPRF-LGPLKGTTKILEGQRAHFEARVE--PQSDLGLVIEWYHNGRSITAANRIQTYYDFGYVALDISQVRAEDAGVYLVVARNKLGEAQQQATMIV 1131 HSP 16 Score: 123.25 bits (308), Expect = 4.586e-28 Identity = 133/535 (24.86%), Postives = 223/535 (41.68%), Query Frame = 3
Query: 678 PKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVT-----EWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEES---------------IITKSNLTESSISQLKSLEN------PGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQS-KGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMN-ITAGEGGPAKFLVKVS--GFPTPTLEWRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLTVI 2192
P+F+ + +++T+ +G+P+ + S +++ T W KDG+ + + V D G L+I D G Y C A+N G ++ P E +I + I L+ +E PGEE V P PP FI P+ + + +EG + + I+P + +WY NG + +R T + AL++L + D+G Y+C + + G ES + L+V SI Q+ + I+ LE + E DE + P FI+ L + + EG +V +V P ND S+ VEWYK+ I SR+ ++ G +LNI+ +ED+G Y + A N+ G+ S+ +I ++ + +++++LE+ S Q E + P F N + E A F ++ G T +EW D + S TY++ L I + + A N +G A LTVI
Sbjct: 486 PQFVQRF-STMTVREGEPITM-------SANAIGTPQPRITWQKDGVQISSTAERFVGIDGGATCLEIPRVTANDAGWYQCTAQNIAGSTANRARLYVEVPREQPNYEQRRLNLPRPTKVIEPEPIPGPEIIYLRHVERAKPHLRPGEE------DRVYP-PPQFIIPL--QNVQQTEGGRVHMEARIEPVGDPTMVVEWYLNGRPLAASARATSVFKFGFIALDLLSIMGHDSGEYMCRVTNASGVAESRAI-LSVVQRPSIEQSSQNPNSLQYINQLEDYSRYQRTE-SIDEQLNQAPQFIRPLRDLGEFEEGKNVHFEAQVTPVNDPSMRVEWYKDGLPITASSRITAIFNFGYVSLNILHLRAEDAGTYTVRAVNRIGEAISQS-SIRVHSRSQVTADLGIPEQQRYIEKVEELEDYRKSQQRRHVQ--EAAEAIAPPQFKTPIQNQLDLREHAHAHFEARLEPVGDSTMRVEWLKDGQPLEASSRITTYHNFGYVALTIKQLTIYDAGTYTCRAYNAMGQDTTVAQLTVI 998 HSP 17 Score: 65.4698 bits (158), Expect = 2.774e-10 Identity = 134/681 (19.68%), Postives = 241/681 (35.39%), Query Frame = 3
Query: 363 INWFKDGKLLSAGSRFSTIFDR--GFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYH-------------------------------GMEEDKHAAKTIPK--------FLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYF--KVTIDPG-------NGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQS---------------EKINISCFEGEKIVTASSLLADSAGYQR-LKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAGEGGPAKFLVKVS--GFPTPTLEWRIDDV-LVVPDSVCKTYNDGSINYLEINRCSELG--DIKVHVAATNPLGVAKEEATLTVI 2192
+ W + G L +F ++ G + L I D G+Y C A++ G A I V I PE P +Q I NL+KN + Y G E+D ++ + + +SQ P S LI+G + + L WF +G L + ++ Y G L + D G Y+ +A N G S + P + + QL++ + +PP+F++ REI +EG F +VT +P NG+++ +D H K +G + I+ + L D G+ C+ ++ G V A+EA+LN +++++++ P F+++ + + EG+ +T++ + W K+ I + + + D G L I + D+G Y A N G T + E+ ++ K++ + Y R ++ + L G E+ + P F + N+ EGG ++ G PT +EW ++ L + G ++ ++ S +G + TN GVA+ A L+V+
Sbjct: 133 VTWTRKGAPLLESQKFRMSYNEATGDVSLLINQIGPGDEGEYTCTARNQYGEA---ICSVYIQPEGA-------PMPALQPIQNLEKNIYSNGYSYTSIEEEFRVDTFEYRLLREVSFREAITRRSGYEQDSQLSQELDRNQGPAQAPQISQKPRSSKLIEGSDAVFTARVGSNPKPRLT--WFHNGQRLVASQKYEISYSSGVATLRVKNATARDGGHYTLLAENLQG-CVVSSAVLAVEPAAETAYEPKPVDVMAEQLEAGK---------------ALPPAFVKAFGDREI--TEGRMTRFDCRVTGNPYPEVFWLINGRQVRDDASH-----KILVNESGSHSLMITNVTRL-----DAGAVQCLARNKAGEV----------------------------AIEAQLNV----LEKEQVVA--PQFVQRFSTMT-VREGEPITMSANA--IGTPQPRITWQKDGVQISSTAERFVGIDGGATCLEIPRVTANDAGWYQCTAQNIAGSTANRARLYVEVPREQPNYEQRRLNLPRPTKVIEPEPIPGPEIIYLRHVERAKPHLRPG--------EEDRVYPPPQFIIPLQNVQQTEGGRVHMEARIEPVGDPTMVVEWYLNGRPLAASARATSVFKFG---FIALDLLSIMGHDSGEYMCRVTNASGVAESRAILSVV 725 HSP 18 Score: 60.077 bits (144), Expect = 1.243e-8 Identity = 86/376 (22.87%), Postives = 146/376 (38.83%), Query Frame = 3
Query: 645 MEEDKHAAK---TIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGE-IVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPK--AEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQS 1754
+EE + A K P F++ + S T+ GQ + + L W K+GM ++ + ++ + L I + ED+G Y CVA N+ G+A C+ C I+ S S+ + PG E A P +E + + +V EG+ F+ +D G+ + GE VK + L I P D G+Y CV ++ G ++QT + + P L+AP +K+ + +P+ K T+T KV+ + V+W++ I Q+ +D +A L I + EDSG + + T+ GQ +S
Sbjct: 4498 VEERRRAGKGGIQPPSFVTTI-QSTTVATGQLARFDAKVT--GTRPLDVYWLKNGMKIQPSIKFKMLEEDSVHTLLIIEPFAEDSGRYECVAVNAAGEAR---CDGDC-----IV-------QSPSKPEKPTTPGSEKA-----------PHIVEQLKSQ--TVEEGSKVIFRCRVD---GKPTPTARWMRGENFVKPSRYFQMSRQGEYYQLVISEAFPEDEGTYKCVAENKLG---------SIQTSAQLKVRPIEN-----------LDAPPTITALKDVSVTEGMPAQFK-------------TTVTGKVKATS-----VQWFREGQLIPETPDFQMIFDGNSAVLLIGTTYEEDSGIFTVRVTSSTGQVES 4801
BLAST of Titin vs. Ensembl Fly
Match: sls (gene:FBgn0086906 transcript:FBtr0332683) HSP 1 Score: 261.151 bits (666), Expect = 4.725e-72 Identity = 201/703 (28.59%), Postives = 330/703 (46.94%), Query Frame = 3
Query: 288 ELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGT--YHGMEEDKHAAKTI---------PKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQ-LKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQ-SKGIS---AIHALEAELNAPKAE--MKEDEII----TDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEK-INISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLTVIPMVDFRPDLRHITPENPFK----KMAGLKKVECTSELNDAFKKSK 2315
++V+ E R+ P +D S+ ++WF +GK L AGSR TI D GF++L+I + D+G Y C A + G A + +++ G+ QLP N+ TGT +EE H + + PKF ++ ++L + +G P+H + +E D ++ EWF +G + GSR+ + D G+ ALD+ + Y D+G+Y+C A N G A T S VTC + +I+ +S L E S+ LK LE G + PP F I ++V E F+ ++P L +WY NG+ + + R +M +L+ILY D+G Y+C + +G + T++ + +I+L Q +G+ A+ +EA + +E + ED++ P F+ Q++ L E ++ P D ++ VEW+ N +++ +R Q YD G +N EDSG+Y ATN G+ ++ I +S G IV S L A R++++E AS + + D + P F K T EG PA+F V+V+G P P + W I+ VV S K NDG + +L++ + + KV V A N +G + L V+ D D R++ +P ++A +K +EL F + K
Sbjct: 3547 DMVIGENALAHFECRLQPINDPSMRVDWFHNGKALWAGSRIKTINDFGFVILEIAGCYQRDSGLYTCKATNKHGEATVS-CKLQVKGRQGIVMEPQLP-----------SNFRTGTESLQKLEETMHKREELVTEDEQPNPPKFTEEIKDNLDVPEGGPIHFDCRVEPVGDPTMRIEWFYNGHVMATGSRVHQLNDFGFIALDVDYIYARDSGEYTCRATNKWGTA-TTSAKVTCKGKHNIVYESQLPEGMTSEKLKELER-GRIPEAPKVVEEVFGPPKFTTQIT--SVTVDEAEAVRFECQVEPKTDPSLRVEWYRNGKPLPSGHRYRNIFDMGFVSLDILYVYGEDSGEYVCRAINNYGEDRTR-ATVSCKKLPTILLQNQVPRGMKRSDALTQMEATIKKYTSEVHLTEDDLFDPDRKQPPRFVTQIKEQLTLTEMAVTKFECQLAPVGDPNMKVEWFFNGKPLLHKNRFQPIYDFGYVAMNFGWVYPEDSGEYVCRATNLYGKDETRAIIKVSGKPG--IVYDSQLPAHMQSIDRIREME---ASWQVVPDEVDPDAKPRTKPVFVSKLEPQTVEEGDPARFCVRVTGHPRPRVMWLINGHTVVHGSRYKLTNDG-MFHLDVPKTRQYDTGKVEVIARNSVGESIATTELKVVARSD---DYRNVLKNSPRPWYDYELAAYQKERQENELEKVFDERK 4223 HSP 2 Score: 235.343 bits (599), Expect = 1.792e-63 Identity = 190/684 (27.78%), Postives = 319/684 (46.64%), Query Frame = 3
Query: 297 VDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKA-ETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVT---LNVQTEKSIILDP-QSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSA---GYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAG--------EGGPAKFLVKVSGFPTPTLEWRID-----DVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLTVIPMVDFRPDLRHITPENPFKKMAGLKKVECTS 2285
V E + + R+ P D L + W ++G+ + A +R +T+ D G++ L++ Y +D+G Y C A + G A ++ A + + ++ + TQ E M +I+ L+ + Y EE+++ T P F+++L L++GQ H E +E + D +L EWF +G L G R YD G+ ALDI Y ED+G+Y+C N+LG+A + NVT S I E ++++++ LE+ + ++ P F P+ R V+EGAP + + T+ P N + +WY NG ++T R + AL+ILY DTG+Y+C + G E VT +NV K++ LD ++ + I LE PK ++E P F+ L ++ L EG+ L +VEP ND +L +EW+ N + G R + ++D G L+I+ ED+G Y ATN+ G E +N +C +++ S++ D+ ++++ LE + + + + + P + TA EG A F +V+ P L RI+ L T++ G ++ L+I + V A N LG A L VIP D +H PE + ++K+E T+
Sbjct: 1702 VAENQAVHFEARLIPVGDPKLTVEWLRNGQPIEASNRTTTMHDFGYVALNMKYVNPEDSGTYTCRAVNELGQAVTS-ASLIVQSKTSIQLETQH-EAAMHKIHQLEDH---SRYQRREEEEYTVTTAPVFVTKLIGPSNLVEGQSAHYECRIEPYPDPNLKVEWFHNGKPLSTGHRFRTTYDFGFAALDILTVYAEDSGEYTCRVTNNLGEAINSIVLNVTSR---SSIIHETQHEEALTKIQHLEDTSRFQRKTDEEQFHAERPQFGRPL--RNAKVNEGAPVHLEATLIPVNDPTMKVEWYCNGRPIQTGHRFKTTYDFGFVALDILYAHAEDTGTYMCKAKNAIG----EAVTTCAVNVTANKTLDLDTLDAQRLEKIRQLETYAPPPKPVVEEK---GQKPIFLTPLSNLEHLKEGEHAHLECRVEPINDPNLKIEWFCNGKQLPTGHRYRTTHDFGYVALDILYVYGEDTGTYICKATNQLG----EAVN-TC--NVRVLNRRSMILDTQHPDALEKIQKLESKVPNARTE------------VGDAPISPPHFTAELRGSTEIYEGQTAHFEAQVAPVHDPNL--RIEFYHNGKPLPSASRFHITFDFGYVS-LDITHAVAEDAGEYSVRAVNALGQAVSSTNLRVIPRGTIISDTQH--PEG----LEKIRKLESTA 2340 HSP 3 Score: 222.631 bits (566), Expect = 2.609e-59 Identity = 180/665 (27.07%), Postives = 313/665 (47.07%), Query Frame = 3
Query: 243 DHLYDSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESS--ISQLKSLEN--PGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSK-GISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQE--ECLPNFDVKPMN----ITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLTVIPMVD 2204
D +Y P F L++ + EG + + R+ P D ++ + W+ +G+ L+A +R +++F GFI LD+L + D+G+Y+C + SG A+S A + + ++ +Q P + Q IN L+ + Y E P+F+ L + +G+ +H E + +D S+ EW+KDG+ + SRI I++ GY +L+I ED G Y+ A N +G+A +QS ++ H + + E I +++ LE+ ++ + + PP F PI ++ + E A A+F+ ++P + +W +G+ ++ SR+T AL I D G+Y C ++ G+ ++ L V ++ I+ + Q G+ I LE + E +E+ IT P F+ L+ ++ EG +VEP +D L +EWY N I +R+Q YD G L+I +ED+G Y ++A NK G+ Q + I E + SS+ Y++ ++LE N + D++E + P F V P++ I G+ + ++ G PT +EW + V S +TY D L+I + + + V ATN LG A A + VI D
Sbjct: 612 DRVYPPPQFIIPLQN-VQQTEGGRVHMEARIEPVGDPTMVVEWYLNGRPLAASARATSVFKFGFIALDLLSIMGHDSGEYMCRVTNASGVAESR-AILSVVQRPSIEQSSQNPNSL-QYINQLE---DYSRYQRTESIDEQLNQAPQFIRPLRDLGEFEEGKNVHFEAQVTPVNDPSMRVEWYKDGLPITASSRITAIFNFGYVSLNILHLRAEDAGTYTVRAVNRIGEAISQS-SIRVHSRSQVTADLGIPEQQRYIEKVEELEDYRKSQQRRHVQEAAEAIAPPQFKTPIQ-NQLDLREHAHAHFEARLEPVGDSTMRVEWLKDGQPLEASSRITTYHNFGYVALTIKQLTIYDAGTYTCRAYNAMGQ-DTTVAQLTVISKNEIVSESQHPGGLQKIQHLEDSSRYGRRE-EEETYITQAPRFLGPLKGTTKILEGQRAHFEARVEPQSDLGLVIEWYHNGRSITAANRIQTYYDFGYVALDISQVRAEDAGVYLVVARNKLGEAQQQATMI--VETRSSIDTSSM--HRGLYEKTQNLE------NKPFVEPQYDIEEISKSKPVF-VTPLSDPKPIHDGKNIHLECRLEPMGDPTMRVEWFHNGRPVTVGSRFRTYYDFGFVALDIIKATAADSGEYTVRATNHLGTAHTSACVRVIDHTD 1255 HSP 4 Score: 221.861 bits (564), Expect = 4.094e-59 Identity = 194/666 (29.13%), Postives = 297/666 (44.59%), Query Frame = 3
Query: 261 PTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRIN--PESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTI--PKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNL---TESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQ-SKG----ISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQ-LNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQ-TQSEKINISCFEGEKIVTASSLLAD-SAGYQRLKDLEEDLASGNISTFQNTEDVQ-------EECLPNFDVKPMNITAGEGGPAKFLVKVS--GFPTPTLEWRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLT 2186
P F ++ + +G+ + P +D L++ W+ +GKL+ +R T+ D G++VLDI Y + D+G+YVC A + G + +N GV Y + P+ + Q I L+ G + D A PKF++Q+ + L++GQ H E L +D LV EW+ +G L G R +D G LDI +CY E++G Y ARN G+ T++ ++ C + S+I S L E + ++ +LE P F P++ E ++ EG A+F+ I P + KL +WY NG +K SR + L I P D+G Y C +E+G + T+ +Q ++SII++ Q KG I I LE L + E D+ P FI P D + E +++P ND S+ V+W+ N + GSR++ D G L I DSG Y ATNK+G+ T S K+ + +G IV L ++ G + L+ LEE + TED Q EE N DV EGGP F +V G PT +EW + ++ S ND L+++ + ATN G A A +T
Sbjct: 3130 PRFTSHIQPLEGLKDGQSAHFECTLIPVNDPDLKVEWYHNGKLMRHSNRIKTVSDFGYVVLDISYLQDHDSGEYVCRAWNKYG---EDFTRTTLNCGGRGGVFYDSLQPDSL-QRIRELE------CPQGQQADTSAPLVAEPPKFITQIVDVTKLVEGQSAHFEARLTPITDPDLVVEWYFNGKKLPHGHRFRTFHDFGIVILDILYCYEENSGVYEARARNKYGEDVTRA-SLKCASKSSLILDSQLPRGMEGGLEKIANLEYSMVRTREETTEETKGKAPVFTVPLENIE-NLREGENAHFEARITPADDPKLKVEWYWNGRPLKAGSRFRTFCDFGFVILEISPVYPEDSGEYSCRAINEYGEAVTT-ATMKIQGKRSIIMESQLPKGMEGTIDRIAELEG-LGSRSTEFVPDDDTGKPPEFI--TSPFDMVIGENALAHFECRLQPINDPSMRVDWFHNGKALWAGSRIKTINDFGFVILEIAGCYQRDSGLYTCKATNKHGEATVSCKLQVKGRQG--IVMEPQLPSNFRTGTESLQKLEETMHKREELV---TEDEQPNPPKFTEEIKDNLDVP-------EGGPIHFDCRVEPVGDPTMRIEWFYNGHVMATGSRVHQLNDFGFIALDVDYIYARDSGEYTCRATNKWGTATTSAKVT 3767 HSP 5 Score: 220.32 bits (560), Expect = 1.240e-58 Identity = 171/588 (29.08%), Postives = 275/588 (46.77%), Query Frame = 3
Query: 303 EGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEM---MQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNL---TESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQ-----SKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECL--PNFDVKPMNITAGEGGPAKFLVKVSGFPTPTL--EW 2021
EG+ R+ P D L + W+ +GK L G RF T D G ++LDILY E+++G Y A++ G + A ++ +S + +QLP M +++I NL+ + EE K A P F L N L +G+ H E + D L EW+ +G LK GSR D G+ L+I+ YPED+G+YSC A N G+A T + + + SII +S L E +I ++ LE G + PP FI P ++ + E A A+F+ + P N + DW+HNG+ + SR+ + L I D+G Y C ++HG + L V+ + I+++PQ G ++ LE ++ + + EDE + P F ++++ + EG + +VEP D ++ +EW+ N + + GSR+ D G L++ + DSG+Y ATNK G T + ++C +G+ + S L + ++LK+LE G I V EE P F + ++T E +F +V P+L EW
Sbjct: 3277 EGQSAHFEARLTPITDPDLVVEWYFNGKKLPHGHRFRTFHDFGIVILDILYCYEENSGVYEARARNKYGEDVTR-ASLKCASKSSLILDSQLPRGMEGGLEKIANLEYSMVRTREETTEETKGKA---PVFTVPLENIENLREGENAHFEARITPADDPKLKVEWYWNGRPLKAGSRFRTFCDFGFVILEISPVYPEDSGEYSCRAINEYGEAVT-TATMKIQGKRSIIMESQLPKGMEGTIDRIAELEGLGSRSTEFVPDDDTGKPPEFI--TSPFDMVIGENALAHFECRLQPINDPSMRVDWFHNGKALWAGSRIKTINDFGFVILEIAGCYQRDSGLYTCKATNKHGEA-TVSCKLQVKGRQGIVMEPQLPSNFRTGTESLQKLEETMHKREELVTEDE-QPNPPKFTEEIKDNLDVPEGGPIHFDCRVEPVGDPTMRIEWFYNGHVMATGSRVHQLNDFGFIALDVDYIYARDSGEYTCRATNKWG-TATTSAKVTC-KGKHNIVYESQLPEGMTSEKLKELE----RGRIP---EAPKVVEEVFGPPKFTTQITSVTVDEAEAVRFECQVEPKTDPSLRVEW 3846 HSP 6 Score: 215.312 bits (547), Expect = 5.366e-57 Identity = 148/513 (28.85%), Postives = 253/513 (49.32%), Query Frame = 3
Query: 258 SPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTES------SISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQ-SKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISC 1775
+P F LE+ + EGE R+ PADD L++ W+ +G+ L AGSRF T D GF++L+I +D+G+Y C A + G A + A ++I + + +QLP+ M I+ + + G+ P+F++ P + + + H E L+ +D S+ +WF +G +L GSRI I D G+ L+IA CY D+G Y+C A N G+A T SC + + I+ + L + S+ +L+ + EE ++ P PP F E I + V EG P +F ++P + +W++NG ++ T SR+ + AL++ Y D+G Y C ++ G + + + + +I+ + Q +G+++ E E K E + P F Q+ + ++E ++V +VEP D SL VEWY+N + +G R + +D G +L+I+ EDSG+Y A N G+ ++ + +SC
Sbjct: 3400 APVFTVPLENIENLREGENAHFEARITPADDPKLKVEWYWNGRPLKAGSRFRTFCDFGFVILEISPVYPEDSGEYSCRAINEYGEAVTT-ATMKIQGKRSIIMESQLPKGMEGTIDRIAELEGLGSRSTEFVPDDDTGKPPEFITS-PFDMVIGENALAHFECRLQPINDPSMRVDWFHNGKALWAGSRIKTINDFGFVILEIAGCYQRDSGLYTCKATNKHGEA-TVSCKLQVKGRQGIVMEPQLPSNFRTGTESLQKLEETMHKREELVTEDEQ---PNPPKFTEEIK-DNLDVPEGGPIHFDCRVEPVGDPTMRIEWFYNGHVMATGSRVHQLNDFGFIALDVDYIYARDSGEYTCRATNKWGTATTS-AKVTCKGKHNIVYESQLPEGMTSEKLKELERGRIPEAPKVVEEVFGPPKFTTQITSVT-VDEAEAVRFECQVEPKTDPSLRVEWYRNGKPLPSGHRYRNIFDMGFVSLDILYVYGEDSGEYVCRAINNYGEDRT-RATVSC 3902 HSP 7 Score: 207.223 bits (526), Expect = 2.331e-54 Identity = 198/707 (28.01%), Postives = 309/707 (43.71%), Query Frame = 3
Query: 12 HNGAIFKCITRHK--YSFIF-NLDFIAMTTQESMEYNNNSNFNSVPDEGVIPNGTTMAPHALEVVNQV-------NKRSLA-------SPRRGSDH----LYDSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTI-PKFLSQLPNSLT-LIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSK-GISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEE--------CLPNFDVK---PMNITAGEGGPAKFLVKVSGFPTPTL--EW 2021
HNG +R + Y F F LD I T +S EY + + GT + V++ N++SL S RR + +P F + L + + EG + L R+ P D S+ I WF +GK + G RF ++ ++ LD+L D+G Y C A++ G A ++ + VRI ++ + TQ E +Q+I L E T H E I P+FL+ P SLT +G H E +E +D +L EWFK+G + +G R I+D GY ALDI ED+G Y+C A N +G ETQ + C E I+T + E+ + Q+ LE+ R P F + + + + E A+F+ + P + + +WYHN +K+ SR T AL+I+ TLP D G+Y C ++ G + V + V T+KSI L+ Q + + + LE + + +DE ++ P F + + ++ E +V ++ P D L VEW +N I +R +D G LN+ EDSG Y A N+ GQ VT++SL+ S +L + + + A I ++ Q P F K P N+ EG A + ++ +P P L EW
Sbjct: 1196 HNGRPVTVGSRFRTYYDFGFVALDIIKATAADSGEYTVRATNHL---------GTAHTSACVRVIDHTDVVTETQNEQSLEQIQLLEDSRRRHHQEEDITIMQAPQFTRGLHN-IETIEGTNVHLECRLQPVGDPSMRIEWFVNGKPVKTGHRFRPAYEFDYVALDLLGCYAIDSGVYTCQARNQLGEAVTSCS-VRIIAKNDLILETQN-ESGLQKIQYL----EDSTRHRRSEFVDEVVNIRPRFLTH-PKSLTNTREGGHAHFECKIEPVTDPNLKVEWFKNGRPITVGHRFRPIHDFGYVALDIVHLIAEDSGVYTCRAVNLIGSDETQ-VELQCRSGEQIVTVTQ-NEAGLEQIHYLEDRSRYTRREEIDESTKQAPVFTTSL--KNVEIKENQRAHFECRLIPVSDPSMRVEWYHNNLPLKSGSRFTETNNFGFVALDIMSTLPEDAGTYTCRAYNAVGEAITSAVAV-VHTKKSIYLESQHETALPRLQHLEDGSKRQRISV-QDEFVSQAPVFTMPVRDV-RVAENQAVHFEARLIPVGDPKLTVEWLRNGQPIEASNRTTTMHDFGYVALNMKYVNPEDSGTYTCRAVNELGQA---------------VTSASLIVQSKTSIQL-ETQHEAAMHKIHQLEDHSRYQRREEEEYTVTTAPVFVTKLIGPSNLV--EGQSAHYECRIEPYPDPNLKVEW 1860 HSP 8 Score: 206.838 bits (525), Expect = 2.914e-54 Identity = 161/598 (26.92%), Postives = 280/598 (46.82%), Query Frame = 3
Query: 261 PTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPI-DPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQS-KGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADS---AGYQRLKDLEEDLASGNISTFQNTEDVQEECL---PNFDVKPMNIT-AGEGGPAKFLVKVSGFPTPTL--EW 2021
P F+ ++++L + E R+ P D ++ + W KDG+ L A SR +T + G++ L I D G Y C A + G + +A + + ++ + +Q P +Q+I +L+ ++ Y EE++ P+FL L + +++GQ H E +E SD LV EW+ +G S+ +RI YD GY ALDI+ ED G Y VARN LG+A+ Q+ + S I S++ + ++LEN +Y+ + P F+ P+ DP+ I +G + + ++P + +W+HNG V SR + AL+I+ +D+G Y V + H + V ++ + Q+ + + I LE + +ED I P F + L I+ + EG +V L +++P D S+ +EW+ N + G R + +Y+ L+++ + DSG Y A N+ G+ + SC +I+ + L+ ++ +G Q+++ LE+ ST + +E + P F P ++T EGG A F K+ P L EW
Sbjct: 890 PQFKTPIQNQLDLREHAHAHFEARLEPVGDSTMRVEWLKDGQPLEASSRITTYHNFGYVALTIKQLTIYDAGTYTCRAYNAMG-QDTTVAQLTVISKNEIVSESQHPG-GLQKIQHLE---DSSRYGRREEEETYITQAPRFLGPLKGTTKILEGQRAHFEARVEPQSDLGLVIEWYHNGRSITAANRIQTYYDFGYVALDISQVRAEDAGVYLVVARNKLGEAQQQATMIV--ETRSSIDTSSMHRGLYEKTQNLENKPFVEPQYDIEEISKSKPVFVTPLSDPKPI--HDGKNIHLECRLEPMGDPTMRVEWFHNGRPVTVGSRFRTYYDFGFVALDIIKATAADSGEY-TVRATNHLGTAHTSACVRVIDHTDVVTETQNEQSLEQIQLLEDSRR--RHHQEEDITIMQAPQFTRGLHNIETI-EGTNVHLECRLQPVGDPSMRIEWFVNGKPVKTGHRFRPAYEFDYVALDLLGCYAIDSGVYTCQARNQLGEAVT-----SC--SVRIIAKNDLILETQNESGLQKIQYLED-------STRHRRSEFVDEVVNIRPRFLTHPKSLTNTREGGHAHFECKIEPVTDPNLKVEW 1460 HSP 9 Score: 206.838 bits (525), Expect = 3.047e-54 Identity = 176/665 (26.47%), Postives = 300/665 (45.11%), Query Frame = 3
Query: 252 YDSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPE--EMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDP-QSKGISAIHALE------AELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADS-------AGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNI-TAGEGGPAKFLVKVSGFPTPTL--EWRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLTV 2189
Y P + + L++E + E +P+ L V P +D +L I W+ +GK+L+ GSRF + GF+ +D++ D G Y C A + +G A ++ + E+ ++ TQ P+ E +++I +L+ + G + ++ P+F ++ N + +G+ H E L D S+V EWF +G L+ R+ IY G AL++ ED G Y+C A N G AE SCN+ C ++ P + R+ S I+P++ + +G A+F+ T+ P N L +WYHNG++++ +R+ + L+I Y D+G Y+C ++++G + TLN + D Q + I LE A+ +AP ++ + P FI Q+ + +L EG S ++ P D L VEWY N + +G R + +D G L+I+ E+SG Y A NK G+ + + ++ C + SSL+ DS G +++ +LE + + T + T + + P F V NI EG A F +++ P L EW + + S +T+ D LEI+ + A N G A AT+ +
Sbjct: 2889 YPPPVWTKPLQAEFHLSEAQPIHLEANVEPKEDPNLFIEWYFNGKMLNHGSRFKMTSEFGFVTMDMIEVYARDQGIYTCKAYNKAGEAFTSTTIFCSSKENIIES-TQHPKGAEGLEQIQDLEDSLRKD---GSKPEQPDLGIPPRFTTEFVNIADIGEGELAHFEANLIPVGDQSMVIEWFYNGKVLEASHRVRTIYAFGTVALEVLGTKIEDTGTYTCRATNKHGTAEI-SCNLEC----------------------VDKPRGQKPRFT---------SHIQPLE----GLKDGQSAHFECTLIPVNDPDLKVEWYHNGKLMRHSNRIKTVSDFGYVVLDISYLQDHDSGEYVCRAWNKYGE-DFTRTTLNCGGRGGVFYDSLQPDSLQRIRELECPQGQQADTSAP--------LVAEPPKFITQIVDVTKLVEGQSAHFEARLTPITDPDLVVEWYFNGKKLPHGHRFRTFHDFGIVILDILYCYEENSGVYEARARNKYGEDVT-RASLKC------ASKSSLILDSQLPRGMEGGLEKIANLEYSM----VRTREETTEETKGKAPVFTVPLENIENLREGENAHFEARITPADDPKLKVEWYWNGRPLKAGSRFRTFCDFGFVILEISPVYPEDSGEYSCRAINEYGEAVTTATMKI 3493 HSP 10 Score: 201.83 bits (512), Expect = 1.281e-52 Identity = 176/599 (29.38%), Postives = 274/599 (45.74%), Query Frame = 3
Query: 75 FIAMTTQESMEYNNNSNFNSVPDEGVIPNGTTMAPHALEVVNQVNKRSLASPRRGSDHLYDSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKA-ETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPID-PREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQ-SKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQ---TQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLE 1853
+I T + E N N + +I + T P ALE + ++ + + D P F +L + EG+ +V P D +L I ++ +GK L + SRF FD G++ LDI + + +D G+Y A + G A S+ ++R+ P + TQ PE +++I L+ T + E + + P F L N + + Q H E L D +L EW+++ ++ SRI +D G+ +LDI+ ED G Y C A N LG+A T S V E+ I SIS++ LE P R + + P F + + P E+ EG A+F+ + P L +W+ NG ++ SRL + L+I +P D G Y+C ++ G S + V+T+ +I P + AI EA +N EM D P F L+ D+L+EG V L +VEP D +L +EW+KN + GSR++ ++D G TL+I ++DS Y ATN+ G+ T S KI E + A SL DS R+ +LE
Sbjct: 2162 YICKATNQLGEAVNTCNVRVLNRRSMILD--TQHPDALEKIQKLESKVPNARTEVGDAPISPPHFTAELRGSTEIYEGQTAHFEAQVAPVHDPNLRIEFYHNGKPLPSASRFHITFDFGYVSLDITHAVAEDAGEYSVRAVNALGQAVSS-TNLRVIPRGTIISDTQHPE-GLEKIRKLES---TAPHQRQEPETPGTRQRPVFTQPLQNIDRINEHQTAHFEARLIPVGDPNLKVEWYRNEKIIEDSSRITKQHDFGFVSLDISHIRKEDEGVYMCRAVNPLGEAVTTASMRVVS---EASIQMDTQHPDSISRIHQLEKPL--APRPTEPERLFEKPIFTQLLTGPSELW--EGTHAHFEARVVPVGDPSLKFEWFINGVELQMGSRLRTTHDFGFVTLDITAVVPEDAGVYMCRAYNAAGEAVSS-TAMKVKTKSNIDGQPLIPESWEAIRLKEAAMNR-VPEMFVDSTPQQAPVFTTHLQSYDKLHEGQHVLLEAQVEPRADPNLRIEWFKNGISLTTGSRIRSTFDFGLVTLSINGLRADDSAIYTCKATNQVGEAVSTSSLKI-----EDRHWLQAESLHPDS--LPRIGELE 2737 HSP 11 Score: 200.675 bits (509), Expect = 2.658e-52 Identity = 171/634 (26.97%), Postives = 289/634 (45.58%), Query Frame = 3
Query: 168 TMAPHALEVVNQVNKRSLASPRRGSDHLYDSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEM----MQE--INNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKA-ETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQ----SKGISAIHALEAEL-NAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNIT-AGEGGPAKF---LVKVSGFPTPTLEW 2021
T P ++ ++Q+ K LA + L++ P F Q L + EG RV P D SL+ WF +G L GSR T D GF+ LDI + +D G Y+C A + +G A S+ A +++ +S +D +PE ++E +N + + + T + P F + L + L +GQ + +E +E +D +L EWFK+G+SL GSRI +D G L I +D+ Y+C A N +G+A T S + + + +L S+ ++ LE P E R P FI ++ I E F+ ++P +S +W++NG+ ++ ++ + AL++ + ++G Y C + G + TL K++ LD Q G+ A+ E EL N ++ + E P + K L+ L+E + L VEP D +L++EWY N + +GSR +++ + G T++++ + D G Y A NK G+ + I C E I+ ++ + G ++++DLE+ L Q + P F + +NI GEG A F L+ V G + +EW
Sbjct: 2458 TQHPDSISRIHQLEK-PLAPRPTEPERLFEKPIFTQLLTGPSELWEGTHAHFEARVVPVGDPSLKFEWFINGVELQMGSRLRTTHDFGFVTLDITAVVPEDAGVYMCRAYNAAGEAVSSTA-MKVKTKSNIDGQPLIPESWEAIRLKEAAMNRVPEMFVDST----------PQQAPVFTTHLQSYDKLHEGQHVLLEAQVEPRADPNLRIEWFKNGISLTTGSRIRSTFDFGLVTLSINGLRADDSAIYTCKATNQVGEAVSTSSLKIE---DRHWLQAESLHPDSLPRIGELEAPKE--GRPEAPEPTYETPVFITHLN--NIECKESDNVRFECNVEPARDPTMSIEWFYNGQPLQAAAKFKSIYDFGYCALDLTNSYAENSGVYTCKATNSKGSATTS-GTLKCTGGKTMFLDTQHPQGEAGLEAVQETEEELANRYTSKTTKPETQYPPPVWTKPLQAEFHLSEAQPIHLEANVEPKEDPNLFIEWYFNGKMLNHGSRFKMTSEFGFVTMDMIEVYARDQGIYTCKAYNKAGEAFT-STTIFCSSKENIIESTQHPKGAEGLEQIQDLEDSLRKDGSKPEQPDLGIP----PRFTTEFVNIADIGEGELAHFEANLIPV-GDQSMVIEW 3065 HSP 12 Score: 196.438 bits (498), Expect = 6.026e-51 Identity = 142/516 (27.52%), Postives = 251/516 (48.64%), Query Frame = 3
Query: 243 DHLYDSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQE-INNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTES-----SISQLK-SLENPGEEYARYNDTSVIP---MPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQ----SKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQT 1748
D + P F ++++ L V EG P+ RV P D ++ I WF +G +++ GSR + D GFI LD+ Y D+G+Y C A + GTA ++ A V + + Y +QLPE M E + L++ +EE PKF +Q+ S+T+ + + + E +E +D SL EW+++G L G R I+D G+ +LDI + Y ED+G+Y C A N+ G+ T++ V+C +I+ ++ + +++Q++ +++ E D P PP F+ I +++++E A F+ + P + +W+ NG+ + +R + A+N + P D+G Y+C + +G+ E+ + + V + I+ D Q + I I +EA E+ D P F+ +LEP + EGD V+V V W N + +++GSR +++ D G L++ D+G +IA N G++
Sbjct: 3670 DEQPNPPKFTEEIKDNLDVPEGGPIHFDCRVEPVGDPTMRIEWFYNGHVMATGSRVHQLNDFGFIALDVDYIYARDSGEYTCRATNKWGTATTS-AKVTCKGKHNIVYESQLPEGMTSEKLKELERGRIPEAPKVVEE----VFGPPKFTTQI-TSVTVDEAEAVRFECQVEPKTDPSLRVEWYRNGKPLPSGHRYRNIFDMGFVSLDILYVYGEDSGEYVCRAINNYGEDRTRA-TVSCKKLPTILLQNQVPRGMKRSDALTQMEATIKKYTSEVHLTEDDLFDPDRKQPPRFVTQIK-EQLTLTEMAVTKFECQLAPVGDPNMKVEWFFNGKPLLHKNRFQPIYDFGYVAMNFGWVYPEDSGEYVCRATNLYGKDETRAI-IKVSGKPGIVYDSQLPAHMQSIDRIREMEASWQVVPDEVDPDAKPRTKPVFVSKLEP-QTVEEGDPARFCVRV--TGHPRPRVMWLINGHTVVHGSRYKLTND-GMFHLDVPKTRQYDTGKVEVIARNSVGES 4172 HSP 13 Score: 183.726 bits (465), Expect = 8.320e-47 Identity = 139/515 (26.99%), Postives = 239/515 (46.41%), Query Frame = 3
Query: 258 SPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYA-RYNDTSVIPM----PPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQ----SKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISC 1775
+P F L+S + EG+ + L +V P D +L I WFK+G L+ GSR + FD G + L I DD+ Y C A + G A S + ++I + + P+ + + I L+ E G E P F++ L N++ + + E +E D ++ EWF +G L+ ++ IYD GYCALD+ Y E++G Y+C A NS G A T S + C +++ + + + L++++ EE A RY + P PP + +P+ E +SE P + + ++P L +WY NG+++ SR E ++++ D G Y C +++ G + T+ ++++II Q ++G+ I LE L ++ ++ ++ P F + I + EG+ + P D S+ +EW+ N + R++ Y GT L ++ ED+G Y ATNK+G + ISC
Sbjct: 2621 APVFTTHLQSYDKLHEGQHVLLEAQVEPRADPNLRIEWFKNGISLTTGSRIRSTFDFGLVTLSINGLRADDSAIYTCKATNQVGEAVST-SSLKIEDRHWLQAESLHPDSLPR-IGELEAPKE-----GRPEAPEPTYETPVFITHL-NNIECKESDNVRFECNVEPARDPTMSIEWFYNGQPLQAAAKFKSIYDFGYCALDLTNSYAENSGVYTCKATNSKGSA-TTSGTLKCTGGKTMFLDTQHPQGE-AGLEAVQETEEELANRYTSKTTKPETQYPPPVWTKPLQ-AEFHLSEAQPIHLEANVEPKEDPNLFIEWYFNGKMLNHGSRFKMTSEFGFVTMDMIEVYARDQGIYTCKAYNKAGEAFTS-TTIFCSSKENIIESTQHPKGAEGLEQIQDLEDSLRKDGSKPEQPDLGIP-PRFTTEFVNIADIGEGELAHFEANLIPVGDQSMVIEWFYNGKVLEASHRVRTIYAFGTVALEVLGTKIEDTGTYTCRATNKHGTAE-----ISC 3117 HSP 14 Score: 155.221 bits (391), Expect = 7.697e-38 Identity = 120/399 (30.08%), Postives = 190/399 (47.62%), Query Frame = 3
Query: 144 EGVIPNGTTMAPHALEVVNQVNKRSLASPRRGSDHLYDSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEM-----MQEINNLDKNYETGTYHGMEEDKHAA--KTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQS-CNVTCHPEESIITKSNLTE--SSISQLKSLENPGEEYARYNDTSVIP-MPPSFIEPIDPREISVSEGAPAYF--KVTIDP---------------GNGQKLSNDWYHNGEIVKTCSRLTGKLEM 1256
E +P G T E + ++ + + + + ++ P F + S + VDE E +R +V P D SL + W+++GK L +G R+ IFD GF+ LDILY +D+G+YVC A ++ G ++ A V + Q+P M + ++ K Y T H E+D K P+F++Q+ LTL + E L D ++ EWF +G L +R IYD GY A++ + YPED+G+Y C A N GK ET++ V+ P I+ S L SI +++ +E + D P P F+ ++P+ +V EG PA F +VT P G+ KL+ND + ++ KT TGK+E+
Sbjct: 3777 ESQLPEGMTS-----EKLKELERGRIPEAPKVVEEVFGPPKFTTQITS-VTVDEAEAVRFECQVEPKTDPSLRVEWYRNGKPLPSGHRYRNIFDMGFVSLDILYVYGEDSGEYVCRAINNYGEDRTR-ATVSCKKLPTILLQNQVPRGMKRSDALTQMEATIKKY-TSEVHLTEDDLFDPDRKQPPRFVTQIKEQLTLTEMAVTKFECQLAPVGDPNMKVEWFFNGKPLLHKNRFQPIYDFGYVAMNFGWVYPEDSGEYVCRATNLYGKDETRAIIKVSGKP--GIVYDSQLPAHMQSIDRIREMEASWQVVPDEVDPDAKPRTKPVFVSKLEPQ--TVEEGDPARFCVRVTGHPRPRVMWLINGHTVVHGSRYKLTNDGMFHLDVPKTRQYDTGKVEV 4163 HSP 15 Score: 147.132 bits (370), Expect = 2.475e-35 Identity = 169/677 (24.96%), Postives = 290/677 (42.84%), Query Frame = 3
Query: 258 SPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDG-KLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRIN-PESGVDYLTQ-----LPEEMMQ-------EINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQS-CNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIP----MPPSFIEPIDPREI-SVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILD----PQSKGISAIHALE-AELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAG--EGGPAKFLVKVS-----GFPTPTLEWRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVH-VAATNPLGVAKEEATLTV 2189
+P F Q S + V EGEP+ + I W KDG ++ S RF I D G L+I +D G Y C A++ +G+ +N A + + P +Y + P ++++ EI L ++ E H ++ P+F+ L N + +G +H+E +E D ++V EW+ +G L +R ++ G+ ALD+ D+G+Y C N+ G AE+++ +V P I +S+ +S+ + LE+ Y+RY T I P FI P+ R++ EG +F+ + P N + +WY +G + SR+T +LNIL+ D G+Y + G S+ ++ V + + D Q + I + LE + + ++E P F ++ L E ++EP D+++ VEW K+ + SR+ ++ G L I D+G Y A N GQ + ++ +IV+ S G Q+++ LE+ G + E+ P F + P+ T EG A F +V G +EW + + + +TY D L+I++ D V+ V A N LG A+++AT+ V
Sbjct: 470 APQFVQRF-STMTVREGEPITMSANA--IGTPQPRITWQKDGVQISSTAERFVGI-DGGATCLEIPRVTANDAGWYQCTAQNIAGST-ANRARLYVEVPREQPNYEQRRLNLPRPTKVIEPEPIPGPEIIYL-RHVERAKPHLRPGEEDRVYPPPQFIIPLQN-VQQTEGGRVHMEARIEPVGDPTMVVEWYLNGRPLAASARATSVFKFGFIALDLLSIMGHDSGEYMCRVTNASGVAESRAILSVVQRPS---IEQSSQNPNSLQYINQLED----YSRYQRTESIDEQLNQAPQFIRPL--RDLGEFEEGKNVHFEAQVTPVNDPSMRVEWYKDGLPITASSRITAIFNFGYVSLNILHLRAEDAGTYTVRAVNRIGEAISQ-SSIRVHSRSQVTADLGIPEQQRYIEKVEELEDYRKSQQRRHVQEAAEAIAPPQFKTPIQNQLDLREHAHAHFEARLEPVGDSTMRVEWLKDGQPLEASSRITTYHNFGYVALTIKQLTIYDAGTYTCRAYNAMGQDTT-VAQLTVISKNEIVSESQ---HPGGLQKIQHLEDSSRYGR----REEEETYITQAPRF-LGPLKGTTKILEGQRAHFEARVEPQSDLGL---VIEWYHNGRSITAANRIQTYYDFGYVALDISQVR-AEDAGVYLVVARNKLGEAQQQATMIV 1116 HSP 16 Score: 120.553 bits (301), Expect = 4.017e-27 Identity = 130/530 (24.53%), Postives = 218/530 (41.13%), Query Frame = 3
Query: 678 PKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEES---------------IITKSNLTESSISQLKSLEN------PGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQS-KGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMN-ITAGEGGPAKFLVKVS--GFPTPTLEWRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLTVI 2192
P+F+ + +++T+ +G+P+ + + W KDG+ + + V D G L+I D G Y C A+N G ++ P E +I + I L+ +E PGEE Y PP FI P+ + + +EG + + I+P + +WY NG + +R T + AL++L + D+G Y+C + + G ES + L+V SI Q+ + I+ LE + E DE + P FI+ L + + EG +V +V P ND S+ VEWYK+ I SR+ ++ G +LNI+ +ED+G Y + A N+ G+ S+ +I ++ + +++++LE+ S Q E + P F N + E A F ++ G T +EW D + S TY++ L I + + A N +G A LTVI
Sbjct: 471 PQFVQRF-STMTVREGEPITMSANAIGTPQPRIT--WQKDGVQISSTAERFVGIDGGATCLEIPRVTANDAGWYQCTAQNIAGSTANRARLYVEVPREQPNYEQRRLNLPRPTKVIEPEPIPGPEIIYLRHVERAKPHLRPGEEDRVYP-------PPQFIIPL--QNVQQTEGGRVHMEARIEPVGDPTMVVEWYLNGRPLAASARATSVFKFGFIALDLLSIMGHDSGEYMCRVTNASGVAESRAI-LSVVQRPSIEQSSQNPNSLQYINQLEDYSRYQRTESI-DEQLNQAPQFIRPLRDLGEFEEGKNVHFEAQVTPVNDPSMRVEWYKDGLPITASSRITAIFNFGYVSLNILHLRAEDAGTYTVRAVNRIGEAISQS-SIRVHSRSQVTADLGIPEQQRYIEKVEELEDYRKSQQRRHVQ--EAAEAIAPPQFKTPIQNQLDLREHAHAHFEARLEPVGDSTMRVEWLKDGQPLEASSRITTYHNFGYVALTIKQLTIYDAGTYTCRAYNAMGQDTTVAQLTVI 983 HSP 17 Score: 79.337 bits (194), Expect = 1.677e-14 Identity = 103/386 (26.68%), Postives = 160/386 (41.45%), Query Frame = 3
Query: 1083 PPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQL-EPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYD-RGTATLNIVSAISEDSGDYWLIATNKNGQTQSE-KINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEWRIDD--VLVVPDSVCKTYNDGSINYLEINRC--SELGDIKVHVAATNPLGVAKEEATLTVIPMV---DFR 2210
PP FI+P++P+ E A V +P + S W+ + E +K+ + + + N S L I G + C +E V +V + ++ L PQ + E +P+ F+++L +P++ ++ G+++ LT +V + VEWY N I IS D +G L I E+ G Y +ATNK G++ S+ + I FE + DS L EEDL +S + E+ P I EG AK VKV G P P ++W DD + + + + DG+ + L IN +LG I A NPLGVA A V +V D+R
Sbjct: 6840 PPRFIQPLEPKYFGEHEVAIIEAIVESEPLS----SFQWFVHNEPIKSSNEVRIVSQANKSTLLIENFQSKFVGPFTC---------RAENVGGSVTSTATVNLIPQEEA--------EEFESPR--------------FVEELVQPVEVMD-GEALLLTCQV--TGKPTPKVEWYHNAEKITENKETTISQDLQGVCQLQITEVFPENEGQYECVATNKIGKSVSKTNVKIQAFE---------YIPDSE-ITGLTGSEEDLLDRTLS-------IDEQAPKIIKKLPEKIEPKEGEQAKLEVKVVGKPKPKVKWLRDDEQIFASEEYQIENFEDGT-SVLVINHVYPDDLGTISFE--AYNPLGVAVTTALFAVEGIVGSKDYR 7167 HSP 18 Score: 65.4698 bits (158), Expect = 2.543e-10 Identity = 104/512 (20.31%), Postives = 182/512 (35.55%), Query Frame = 3
Query: 363 INWFKDGKLLSAGSRFSTIFDR--GFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYH-------------------------------GMEEDKHAAKTIPK--------FLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYF--KVTIDPG-------NGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQT 1748
+ W + G L +F ++ G + L I D G+Y C A++ G A I V I PE P +Q I NL+KN + Y G E+D ++ + + +SQ P S LI+G + + L WF +G L + ++ Y G L + D G Y+ +A N G S + P + + QL++ + +PP+F++ REI +EG F +VT +P NG+++ +D H K +G + I+ + L D G+ C+ ++ G V A+EA+LN +++++++ P F+++ + + EG+ +T++ + W K+ I + + + D G L I + D+G Y A N G T
Sbjct: 118 VTWTRKGAPLLESQKFRMSYNEATGDVSLLINQIGPGDEGEYTCTARNQYGEA---ICSVYIQPEGA-------PMPALQPIQNLEKNIYSNGYSYTSIEEEFRVDTFEYRLLREVSFREAITRRSGYEQDSQLSQELDRNQGPAQAPQISQKPRSSKLIEGSDAVFTARVGSNPKPRLT--WFHNGQRLVASQKYEISYSSGVATLRVKNATARDGGHYTLLAENLQGCV-VSSAVLAVEPAAETAYEPKPVDVMAEQLEAGKA---------------LPPAFVKAFGDREI--TEGRMTRFDCRVTGNPYPEVFWLINGRQVRDDASH-----KILVNESGSHSLMITNVTRL-----DAGAVQCLARNKAGEV----------------------------AIEAQLNV----LEKEQVVA--PQFVQRFSTMT-VREGEPITMSANA--IGTPQPRITWQKDGVQISSTAERFVGIDGGATCLEIPRVTANDAGWYQCTAQNIAGST 552 HSP 19 Score: 62.3882 bits (150), Expect = 2.065e-9 Identity = 102/485 (21.03%), Postives = 176/485 (36.29%), Query Frame = 3
Query: 363 INWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAK---TIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGE-IVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPK--AEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQS------EKINISCFE 1781
+ W+++ + G +L I +D+ + CVA++ GTA+ + V +EE + A K P F++ + S T+ GQ + + L W K+GM ++ + ++ + L I + ED+G Y CVA N+ G+A C+ C I+ S S+ + PG E A P +E + + +V EG+ F+ +D G+ + GE VK + L I P D G+Y CV ++ G ++QT + + P L+AP +K+ + +P+ K T+T KV + V+W++ I Q+ +D +A L I + EDSG + + T+ GQ +S +K IS F+
Sbjct: 4425 VKWYRESFPIQNSPDLQIHTFSGKSILIIRQVFVEDSAVFSCVAENRGGTAKCSANLV------------------------------------VEERRRAGKGGIQPPSFVTTI-QSTTVATGQLARFDAKV--TGTRPLDVYWLKNGMKIQPSIKFKMLEEDSVHTLLIIEPFAEDSGRYECVAVNAAGEA---RCDGDC-----IV-------QSPSKPEKPTTPGSEKA-----------PHIVEQL--KSQTVEEGSKVIFRCRVD---GKPTPTARWMRGENFVKPSRYFQMSRQGEYYQLVISEAFPEDEGTYKCVAENKLG---------SIQTSAQLKVRPIEN-----------LDAPPTITALKDVSVTEGMPAQFK-------------TTVTGKV-----KATSVQWFREGQLIPETPDFQMIFDGNSAVLLIGTTYEEDSGIFTVRVTSSTGQVESSAKLTVKKRRISAFQ 4801 HSP 20 Score: 54.6842 bits (130), Expect = 5.516e-7 Identity = 54/224 (24.11%), Postives = 86/224 (38.39%), Query Frame = 3
Query: 1527 QLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPM-NITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLVVPDSVCKTY-NDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLTVI 2192
+L EG T +V ++ + W+ N ++ + +ISY G ATL + +A + D G Y L+A N G C ++ + + + + E L +G LP VK + EG +F +V+G P P + W I+ V D+ K N+ + L I + L V A N G EA L V+
Sbjct: 265 KLIEGSDAVFTARV--GSNPKPRLTWFHNGQRLVASQKYEISYSSGVATLRVKNATARDGGHYTLLAENLQG----------CVVSSAVLAVEPAAETAYEPKPVDVMAEQLEAGK-------------ALPPAFVKAFGDREITEGRMTRFDCRVTGNPYPEVFWLINGRQVRDDASHKILVNESGSHSLMITNVTRLDAGAVQCLARNKAGEVAIEAQLNVL 463 HSP 21 Score: 50.8322 bits (120), Expect = 6.873e-6 Identity = 108/528 (20.45%), Postives = 196/528 (37.12%), Query Frame = 3
Query: 651 EDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCA-LDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTI----DPG-----NGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQL--NEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLVVP-DSVCKTYNDGSINYL--EINRCSELGDIKVHVAATNPLGVAKEEATLTV 2189
EDK A P+F+ +L + G + E +E + + WF++ +K + YD A L I +PED G ++ VA+N+ G + + +I +S L++ S +L + +PP+F + P+ V E + + +P NG+ + + N IV + M S ++I S G+Y + + G P+TL V+T + AP+ LEP+ + EG+SV L+ ++ + V WYK+ + N D+ TL +++ + G+Y + A N G ++ TA+ + + AG GN E +E+ +P + G + KVSG P P ++W ++ + P + + + Y+ +I + + N ++ GD K A+NP+G A + V
Sbjct: 8999 EDKPIA---PRFIEKL-QPIHTPDGYTVQFECKVEGNPRPQIA--WFRETAIIKPSQDFQMFYDDDNVATLIIREVFPEDAGQFTVVAKNAAGFTSSTT---------ELIVESPLSDHG-SDATALSRRSMSRESSLADILEGIPPTFSK--KPKAQYVDENTNVILECRLVAVPEPDIVWTFNGEDIDEEEIKNVRIVTESD-----MHMYCSVVHISKVKKSQEGTYEVIATNREGEARL-PITLKVRT--------------------TDKEAPQI-----------------LEPLRNMVIREGESVVLSTQI--VGNPPPKVTWYKDGKPVKNAKS-----DKDLHTLTLITPQKSEKGEYTVKAVNPLGSVET--------------TANLTIEEPAG-------------GNAEPPLFVERFEEQNVP------------QKGEIRLPAKVSGNPVPEVQWLFNNTPLFPSERIQQVYDGENIELIIKDANPETDSGDYK--CIASNPIGKTSHGARVIV 9417
BLAST of Titin vs. Ensembl Fly
Match: sls (gene:FBgn0086906 transcript:FBtr0332679) HSP 1 Score: 258.84 bits (660), Expect = 2.793e-71 Identity = 201/703 (28.59%), Postives = 330/703 (46.94%), Query Frame = 3
Query: 288 ELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGT--YHGMEEDKHAAKTI---------PKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQ-LKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQ-SKGIS---AIHALEAELNAPKAE--MKEDEII----TDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEK-INISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLTVIPMVDFRPDLRHITPENPFK----KMAGLKKVECTSELNDAFKKSK 2315
++V+ E R+ P +D S+ ++WF +GK L AGSR TI D GF++L+I + D+G Y C A + G A + +++ G+ QLP N+ TGT +EE H + + PKF ++ ++L + +G P+H + +E D ++ EWF +G + GSR+ + D G+ ALD+ + Y D+G+Y+C A N G A T S VTC + +I+ +S L E S+ LK LE G + PP F I ++V E F+ ++P L +WY NG+ + + R +M +L+ILY D+G Y+C + +G + T++ + +I+L Q +G+ A+ +EA + +E + ED++ P F+ Q++ L E ++ P D ++ VEW+ N +++ +R Q YD G +N EDSG+Y ATN G+ ++ I +S G IV S L A R++++E AS + + D + P F K T EG PA+F V+V+G P P + W I+ VV S K NDG + +L++ + + KV V A N +G + L V+ D D R++ +P ++A +K +EL F + K
Sbjct: 3547 DMVIGENALAHFECRLQPINDPSMRVDWFHNGKALWAGSRIKTINDFGFVILEIAGCYQRDSGLYTCKATNKHGEATVS-CKLQVKGRQGIVMEPQLP-----------SNFRTGTESLQKLEETMHKREELVTEDEQPNPPKFTEEIKDNLDVPEGGPIHFDCRVEPVGDPTMRIEWFYNGHVMATGSRVHQLNDFGFIALDVDYIYARDSGEYTCRATNKWGTA-TTSAKVTCKGKHNIVYESQLPEGMTSEKLKELER-GRIPEAPKVVEEVFGPPKFTTQIT--SVTVDEAEAVRFECQVEPKTDPSLRVEWYRNGKPLPSGHRYRNIFDMGFVSLDILYVYGEDSGEYVCRAINNYGEDRTR-ATVSCKKLPTILLQNQVPRGMKRSDALTQMEATIKKYTSEVHLTEDDLFDPDRKQPPRFVTQIKEQLTLTEMAVTKFECQLAPVGDPNMKVEWFFNGKPLLHKNRFQPIYDFGYVAMNFGWVYPEDSGEYVCRATNLYGKDETRAIIKVSGKPG--IVYDSQLPAHMQSIDRIREME---ASWQVVPDEVDPDAKPRTKPVFVSKLEPQTVEEGDPARFCVRVTGHPRPRVMWLINGHTVVHGSRYKLTNDG-MFHLDVPKTRQYDTGKVEVIARNSVGESIATTELKVVARSD---DYRNVLKNSPRPWYDYELAAYQKERQENELEKVFDERK 4223 HSP 2 Score: 234.572 bits (597), Expect = 2.999e-63 Identity = 190/684 (27.78%), Postives = 319/684 (46.64%), Query Frame = 3
Query: 297 VDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKA-ETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVT---LNVQTEKSIILDP-QSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSA---GYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAG--------EGGPAKFLVKVSGFPTPTLEWRID-----DVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLTVIPMVDFRPDLRHITPENPFKKMAGLKKVECTS 2285
V E + + R+ P D L + W ++G+ + A +R +T+ D G++ L++ Y +D+G Y C A + G A ++ A + + ++ + TQ E M +I+ L+ + Y EE+++ T P F+++L L++GQ H E +E + D +L EWF +G L G R YD G+ ALDI Y ED+G+Y+C N+LG+A + NVT S I E ++++++ LE+ + ++ P F P+ R V+EGAP + + T+ P N + +WY NG ++T R + AL+ILY DTG+Y+C + G E VT +NV K++ LD ++ + I LE PK ++E P F+ L ++ L EG+ L +VEP ND +L +EW+ N + G R + ++D G L+I+ ED+G Y ATN+ G E +N +C +++ S++ D+ ++++ LE + + + + + P + TA EG A F +V+ P L RI+ L T++ G ++ L+I + V A N LG A L VIP D +H PE + ++K+E T+
Sbjct: 1702 VAENQAVHFEARLIPVGDPKLTVEWLRNGQPIEASNRTTTMHDFGYVALNMKYVNPEDSGTYTCRAVNELGQAVTS-ASLIVQSKTSIQLETQH-EAAMHKIHQLEDH---SRYQRREEEEYTVTTAPVFVTKLIGPSNLVEGQSAHYECRIEPYPDPNLKVEWFHNGKPLSTGHRFRTTYDFGFAALDILTVYAEDSGEYTCRVTNNLGEAINSIVLNVTSR---SSIIHETQHEEALTKIQHLEDTSRFQRKTDEEQFHAERPQFGRPL--RNAKVNEGAPVHLEATLIPVNDPTMKVEWYCNGRPIQTGHRFKTTYDFGFVALDILYAHAEDTGTYMCKAKNAIG----EAVTTCAVNVTANKTLDLDTLDAQRLEKIRQLETYAPPPKPVVEEK---GQKPIFLTPLSNLEHLKEGEHAHLECRVEPINDPNLKIEWFCNGKQLPTGHRYRTTHDFGYVALDILYVYGEDTGTYICKATNQLG----EAVN-TC--NVRVLNRRSMILDTQHPDALEKIQKLESKVPNARTE------------VGDAPISPPHFTAELRGSTEIYEGQTAHFEAQVAPVHDPNL--RIEFYHNGKPLPSASRFHITFDFGYVS-LDITHAVAEDAGEYSVRAVNALGQAVSSTNLRVIPRGTIISDTQH--PEG----LEKIRKLESTA 2340 HSP 3 Score: 220.32 bits (560), Expect = 1.416e-58 Identity = 180/665 (27.07%), Postives = 313/665 (47.07%), Query Frame = 3
Query: 243 DHLYDSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESS--ISQLKSLEN--PGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSK-GISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQE--ECLPNFDVKPMN----ITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLTVIPMVD 2204
D +Y P F L++ + EG + + R+ P D ++ + W+ +G+ L+A +R +++F GFI LD+L + D+G+Y+C + SG A+S A + + ++ +Q P +Q IN L+ + Y E P+F+ L + +G+ +H E + +D S+ EW+KDG+ + SRI I++ GY +L+I ED G Y+ A N +G+A +QS ++ H + + E I +++ LE+ ++ + + PP F PI ++ + E A A+F+ ++P + +W +G+ ++ SR+T AL I D G+Y C ++ G+ ++ L V ++ I+ + Q G+ I LE + E +E+ IT P F+ L+ ++ EG +VEP +D L +EWY N I +R+Q YD G L+I +ED+G Y ++A NK G+ Q + I E + SS+ Y++ ++LE N + D++E + P F V P++ I G+ + ++ G PT +EW + V S +TY D L+I + + + V ATN LG A A + VI D
Sbjct: 612 DRVYPPPQFIIPLQN-VQQTEGGRVHMEARIEPVGDPTMVVEWYLNGRPLAASARATSVFKFGFIALDLLSIMGHDSGEYMCRVTNASGVAESR-AILSVVQRPSIEQSSQNPN-SLQYINQLE---DYSRYQRTESIDEQLNQAPQFIRPLRDLGEFEEGKNVHFEAQVTPVNDPSMRVEWYKDGLPITASSRITAIFNFGYVSLNILHLRAEDAGTYTVRAVNRIGEAISQS-SIRVHSRSQVTADLGIPEQQRYIEKVEELEDYRKSQQRRHVQEAAEAIAPPQFKTPIQ-NQLDLREHAHAHFEARLEPVGDSTMRVEWLKDGQPLEASSRITTYHNFGYVALTIKQLTIYDAGTYTCRAYNAMGQ-DTTVAQLTVISKNEIVSESQHPGGLQKIQHLEDSSRYGRRE-EEETYITQAPRFLGPLKGTTKILEGQRAHFEARVEPQSDLGLVIEWYHNGRSITAANRIQTYYDFGYVALDISQVRAEDAGVYLVVARNKLGEAQQQATMI--VETRSSIDTSSM--HRGLYEKTQNLE------NKPFVEPQYDIEEISKSKPVF-VTPLSDPKPIHDGKNIHLECRLEPMGDPTMRVEWFHNGRPVTVGSRFRTYYDFGFVALDIIKATAADSGEYTVRATNHLGTAHTSACVRVIDHTD 1255 HSP 4 Score: 219.164 bits (557), Expect = 3.611e-58 Identity = 191/640 (29.84%), Postives = 288/640 (45.00%), Query Frame = 3
Query: 339 PADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRIN--PESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTI--PKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNL---TESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQ-SKG----ISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQ-LNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQ-TQSEKINISCFEGEKIVTASSLLAD-SAGYQRLKDLEEDLASGNISTFQNTEDVQ-------EECLPNFDVKPMNITAGEGGPAKFLVKVS--GFPTPTLEWRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLT 2186
P +D L++ W+ +GKL+ +R T+ D G++VLDI Y + D+G+YVC A + G + +N GV Y + P+ +Q I L+ G + D A PKF++Q+ + L++GQ H E L +D LV EW+ +G L G R +D G LDI +CY E++G Y ARN G+ T++ ++ C + S+I S L E + ++ +LE P F P++ E ++ EG A+F+ I P + KL +WY NG +K SR + L I P D+G Y C +E+G + T+ +Q ++SII++ Q KG I I LE L + E D+ P FI P D + E +++P ND S+ V+W+ N + GSR++ D G L I DSG Y ATNK+G+ T S K+ + +G IV L ++ G + L+ LEE + TED Q EE N DV EGGP F +V G PT +EW + ++ S ND L+++ + ATN G A A +T
Sbjct: 3156 PVNDPDLKVEWYHNGKLMRHSNRIKTVSDFGYVVLDISYLQDHDSGEYVCRAWNKYG---EDFTRTTLNCGGRGGVFYDSLQPD-SLQRIRELE------CPQGQQADTSAPLVAEPPKFITQIVDVTKLVEGQSAHFEARLTPITDPDLVVEWYFNGKKLPHGHRFRTFHDFGIVILDILYCYEENSGVYEARARNKYGEDVTRA-SLKCASKSSLILDSQLPRGMEGGLEKIANLEYSMVRTREETTEETKGKAPVFTVPLENIE-NLREGENAHFEARITPADDPKLKVEWYWNGRPLKAGSRFRTFCDFGFVILEISPVYPEDSGEYSCRAINEYGEAVTT-ATMKIQGKRSIIMESQLPKGMEGTIDRIAELEG-LGSRSTEFVPDDDTGKPPEFI--TSPFDMVIGENALAHFECRLQPINDPSMRVDWFHNGKALWAGSRIKTINDFGFVILEIAGCYQRDSGLYTCKATNKHGEATVSCKLQVKGRQG--IVMEPQLPSNFRTGTESLQKLEETMHKREELV---TEDEQPNPPKFTEEIKDNLDV-------PEGGPIHFDCRVEPVGDPTMRIEWFYNGHVMATGSRVHQLNDFGFIALDVDYIYARDSGEYTCRATNKWGTATTSAKVT 3767 HSP 5 Score: 218.779 bits (556), Expect = 4.170e-58 Identity = 171/588 (29.08%), Postives = 275/588 (46.77%), Query Frame = 3
Query: 303 EGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEM---MQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNL---TESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQ-----SKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECL--PNFDVKPMNITAGEGGPAKFLVKVSGFPTPTL--EW 2021
EG+ R+ P D L + W+ +GK L G RF T D G ++LDILY E+++G Y A++ G + A ++ +S + +QLP M +++I NL+ + EE K A P F L N L +G+ H E + D L EW+ +G LK GSR D G+ L+I+ YPED+G+YSC A N G+A T + + + SII +S L E +I ++ LE G + PP FI P ++ + E A A+F+ + P N + DW+HNG+ + SR+ + L I D+G Y C ++HG + L V+ + I+++PQ G ++ LE ++ + + EDE + P F ++++ + EG + +VEP D ++ +EW+ N + + GSR+ D G L++ + DSG+Y ATNK G T + ++C +G+ + S L + ++LK+LE G I V EE P F + ++T E +F +V P+L EW
Sbjct: 3277 EGQSAHFEARLTPITDPDLVVEWYFNGKKLPHGHRFRTFHDFGIVILDILYCYEENSGVYEARARNKYGEDVTR-ASLKCASKSSLILDSQLPRGMEGGLEKIANLEYSMVRTREETTEETKGKA---PVFTVPLENIENLREGENAHFEARITPADDPKLKVEWYWNGRPLKAGSRFRTFCDFGFVILEISPVYPEDSGEYSCRAINEYGEAVT-TATMKIQGKRSIIMESQLPKGMEGTIDRIAELEGLGSRSTEFVPDDDTGKPPEFI--TSPFDMVIGENALAHFECRLQPINDPSMRVDWFHNGKALWAGSRIKTINDFGFVILEIAGCYQRDSGLYTCKATNKHGEA-TVSCKLQVKGRQGIVMEPQLPSNFRTGTESLQKLEETMHKREELVTEDE-QPNPPKFTEEIKDNLDVPEGGPIHFDCRVEPVGDPTMRIEWFYNGHVMATGSRVHQLNDFGFIALDVDYIYARDSGEYTCRATNKWG-TATTSAKVTC-KGKHNIVYESQLPEGMTSEKLKELE----RGRIP---EAPKVVEEVFGPPKFTTQITSVTVDEAEAVRFECQVEPKTDPSLRVEW 3846 HSP 6 Score: 213.001 bits (541), Expect = 3.280e-56 Identity = 148/513 (28.85%), Postives = 253/513 (49.32%), Query Frame = 3
Query: 258 SPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTES------SISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQ-SKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISC 1775
+P F LE+ + EGE R+ PADD L++ W+ +G+ L AGSRF T D GF++L+I +D+G+Y C A + G A + A ++I + + +QLP+ M I+ + + G+ P+F++ P + + + H E L+ +D S+ +WF +G +L GSRI I D G+ L+IA CY D+G Y+C A N G+A T SC + + I+ + L + S+ +L+ + EE ++ P PP F E I + V EG P +F ++P + +W++NG ++ T SR+ + AL++ Y D+G Y C ++ G + + + + +I+ + Q +G+++ E E K E + P F Q+ + ++E ++V +VEP D SL VEWY+N + +G R + +D G +L+I+ EDSG+Y A N G+ ++ + +SC
Sbjct: 3400 APVFTVPLENIENLREGENAHFEARITPADDPKLKVEWYWNGRPLKAGSRFRTFCDFGFVILEISPVYPEDSGEYSCRAINEYGEAVTT-ATMKIQGKRSIIMESQLPKGMEGTIDRIAELEGLGSRSTEFVPDDDTGKPPEFITS-PFDMVIGENALAHFECRLQPINDPSMRVDWFHNGKALWAGSRIKTINDFGFVILEIAGCYQRDSGLYTCKATNKHGEA-TVSCKLQVKGRQGIVMEPQLPSNFRTGTESLQKLEETMHKREELVTEDEQ---PNPPKFTEEIK-DNLDVPEGGPIHFDCRVEPVGDPTMRIEWFYNGHVMATGSRVHQLNDFGFIALDVDYIYARDSGEYTCRATNKWGTATTS-AKVTCKGKHNIVYESQLPEGMTSEKLKELERGRIPEAPKVVEEVFGPPKFTTQITSVT-VDEAEAVRFECQVEPKTDPSLRVEWYRNGKPLPSGHRYRNIFDMGFVSLDILYVYGEDSGEYVCRAINNYGEDRT-RATVSC 3902 HSP 7 Score: 206.068 bits (523), Expect = 5.058e-54 Identity = 198/707 (28.01%), Postives = 309/707 (43.71%), Query Frame = 3
Query: 12 HNGAIFKCITRHK--YSFIF-NLDFIAMTTQESMEYNNNSNFNSVPDEGVIPNGTTMAPHALEVVNQV-------NKRSLA-------SPRRGSDH----LYDSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTI-PKFLSQLPNSLT-LIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSK-GISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEE--------CLPNFDVK---PMNITAGEGGPAKFLVKVSGFPTPTL--EW 2021
HNG +R + Y F F LD I T +S EY + + GT + V++ N++SL S RR + +P F + L + + EG + L R+ P D S+ I WF +GK + G RF ++ ++ LD+L D+G Y C A++ G A ++ + VRI ++ + TQ E +Q+I L E T H E I P+FL+ P SLT +G H E +E +D +L EWFK+G + +G R I+D GY ALDI ED+G Y+C A N +G ETQ + C E I+T + E+ + Q+ LE+ R P F + + + + E A+F+ + P + + +WYHN +K+ SR T AL+I+ TLP D G+Y C ++ G + V + V T+KSI L+ Q + + + LE + + +DE ++ P F + + ++ E +V ++ P D L VEW +N I +R +D G LN+ EDSG Y A N+ GQ VT++SL+ S +L + + + A I ++ Q P F K P N+ EG A + ++ +P P L EW
Sbjct: 1196 HNGRPVTVGSRFRTYYDFGFVALDIIKATAADSGEYTVRATNHL---------GTAHTSACVRVIDHTDVVTETQNEQSLEQIQLLEDSRRRHHQEEDITIMQAPQFTRGLHN-IETIEGTNVHLECRLQPVGDPSMRIEWFVNGKPVKTGHRFRPAYEFDYVALDLLGCYAIDSGVYTCQARNQLGEAVTSCS-VRIIAKNDLILETQN-ESGLQKIQYL----EDSTRHRRSEFVDEVVNIRPRFLTH-PKSLTNTREGGHAHFECKIEPVTDPNLKVEWFKNGRPITVGHRFRPIHDFGYVALDIVHLIAEDSGVYTCRAVNLIGSDETQ-VELQCRSGEQIVTVTQ-NEAGLEQIHYLEDRSRYTRREEIDESTKQAPVFTTSL--KNVEIKENQRAHFECRLIPVSDPSMRVEWYHNNLPLKSGSRFTETNNFGFVALDIMSTLPEDAGTYTCRAYNAVGEAITSAVAV-VHTKKSIYLESQHETALPRLQHLEDGSKRQRISV-QDEFVSQAPVFTMPVRDV-RVAENQAVHFEARLIPVGDPKLTVEWLRNGQPIEASNRTTTMHDFGYVALNMKYVNPEDSGTYTCRAVNELGQA---------------VTSASLIVQSKTSIQL-ETQHEAAMHKIHQLEDHSRYQRREEEEYTVTTAPVFVTKLIGPSNLV--EGQSAHYECRIEPYPDPNLKVEW 1860 HSP 8 Score: 205.682 bits (522), Expect = 8.120e-54 Identity = 162/603 (26.87%), Postives = 284/603 (47.10%), Query Frame = 3
Query: 261 PTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSI-----SQLKSLENPGEEYARYNDTSVIPMPPSFIEPI-DPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQS-KGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADS---AGYQRLKDLEEDLASGNISTFQNTEDVQEECL---PNFDVKPMNIT-AGEGGPAKFLVKVSGFPTPTL--EW 2021
P F+ ++++L + E R+ P D ++ + W KDG+ L A SR +T + G++ L I D G Y C A + G + +A + + ++ + +Q P +Q+I +L+ ++ Y EE++ P+FL L + +++GQ H E +E SD LV EW+ +G S+ +RI YD GY ALDI+ ED G Y VARN LG+A+ Q+ + + T+S++ SS+ + ++LEN +Y+ + P F+ P+ DP+ I +G + + ++P + +W+HNG V SR + AL+I+ +D+G Y V + H + V ++ + Q+ + + I LE + +ED I P F + L I+ + EG +V L +++P D S+ +EW+ N + G R + +Y+ L+++ + DSG Y A N+ G+ + SC +I+ + L+ ++ +G Q+++ LE+ ST + +E + P F P ++T EGG A F K+ P L EW
Sbjct: 890 PQFKTPIQNQLDLREHAHAHFEARLEPVGDSTMRVEWLKDGQPLEASSRITTYHNFGYVALTIKQLTIYDAGTYTCRAYNAMG-QDTTVAQLTVISKNEIVSESQHPG-GLQKIQHLE---DSSRYGRREEEETYITQAPRFLGPLKGTTKILEGQRAHFEARVEPQSDLGLVIEWYHNGRSITAANRIQTYYDFGYVALDISQVRAEDAGVYLVVARNKLGEAQQQATMI-------VETRSSIDTSSMHRGLYEKTQNLENKPFVEPQYDIEEISKSKPVFVTPLSDPKPI--HDGKNIHLECRLEPMGDPTMRVEWFHNGRPVTVGSRFRTYYDFGFVALDIIKATAADSGEY-TVRATNHLGTAHTSACVRVIDHTDVVTETQNEQSLEQIQLLEDSRR--RHHQEEDITIMQAPQFTRGLHNIETI-EGTNVHLECRLQPVGDPSMRIEWFVNGKPVKTGHRFRPAYEFDYVALDLLGCYAIDSGVYTCQARNQLGEAVT-----SC--SVRIIAKNDLILETQNESGLQKIQYLED-------STRHRRSEFVDEVVNIRPRFLTHPKSLTNTREGGHAHFECKIEPVTDPNLKVEW 1460 HSP 9 Score: 204.527 bits (519), Expect = 1.629e-53 Identity = 176/665 (26.47%), Postives = 300/665 (45.11%), Query Frame = 3
Query: 252 YDSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPE--EMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDP-QSKGISAIHALE------AELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADS-------AGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNI-TAGEGGPAKFLVKVSGFPTPTL--EWRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLTV 2189
Y P + + L++E + E +P+ L V P +D +L I W+ +GK+L+ GSRF + GF+ +D++ D G Y C A + +G A ++ + E+ ++ TQ P+ E +++I +L+ + G + ++ P+F ++ N + +G+ H E L D S+V EWF +G L+ R+ IY G AL++ ED G Y+C A N G AE SCN+ C ++ P + R+ S I+P++ + +G A+F+ T+ P N L +WYHNG++++ +R+ + L+I Y D+G Y+C ++++G + TLN + D Q + I LE A+ +AP ++ + P FI Q+ + +L EG S ++ P D L VEWY N + +G R + +D G L+I+ E+SG Y A NK G+ + + ++ C + SSL+ DS G +++ +LE + + T + T + + P F V NI EG A F +++ P L EW + + S +T+ D LEI+ + A N G A AT+ +
Sbjct: 2889 YPPPVWTKPLQAEFHLSEAQPIHLEANVEPKEDPNLFIEWYFNGKMLNHGSRFKMTSEFGFVTMDMIEVYARDQGIYTCKAYNKAGEAFTSTTIFCSSKENIIES-TQHPKGAEGLEQIQDLEDSLRKD---GSKPEQPDLGIPPRFTTEFVNIADIGEGELAHFEANLIPVGDQSMVIEWFYNGKVLEASHRVRTIYAFGTVALEVLGTKIEDTGTYTCRATNKHGTAEI-SCNLEC----------------------VDKPRGQKPRFT---------SHIQPLE----GLKDGQSAHFECTLIPVNDPDLKVEWYHNGKLMRHSNRIKTVSDFGYVVLDISYLQDHDSGEYVCRAWNKYGE-DFTRTTLNCGGRGGVFYDSLQPDSLQRIRELECPQGQQADTSAP--------LVAEPPKFITQIVDVTKLVEGQSAHFEARLTPITDPDLVVEWYFNGKKLPHGHRFRTFHDFGIVILDILYCYEENSGVYEARARNKYGEDVT-RASLKC------ASKSSLILDSQLPRGMEGGLEKIANLEYSM----VRTREETTEETKGKAPVFTVPLENIENLREGENAHFEARITPADDPKLKVEWYWNGRPLKAGSRFRTFCDFGFVILEISPVYPEDSGEYSCRAINEYGEAVTTATMKI 3493 HSP 10 Score: 200.29 bits (508), Expect = 3.958e-52 Identity = 170/568 (29.93%), Postives = 263/568 (46.30%), Query Frame = 3
Query: 168 TMAPHALEVVNQVNKRSLASPRRGSDHLYDSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKA-ETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPID-PREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQ-SKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQ---TQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLE 1853
T P ALE + ++ + + D P F +L + EG+ +V P D +L I ++ +GK L + SRF FD G++ LDI + + +D G+Y A + G A S+ ++R+ P + TQ PE +++I L+ T + E + + P F L N + + Q H E L D +L EW+++ ++ SRI +D G+ +LDI+ ED G Y C A N LG+A T S V E+ I SIS++ LE P R + + P F + + P E+ EG A+F+ + P L +W+ NG ++ SRL + L+I +P D G Y+C ++ G S + V+T+ +I P + AI EA +N EM D P F L+ D+L+EG V L +VEP D +L +EW+KN + GSR++ ++D G TL+I ++DS Y ATN+ G+ T S KI E + A SL DS R+ +LE
Sbjct: 2191 TQHPDALEKIQKLESKVPNARTEVGDAPISPPHFTAELRGSTEIYEGQTAHFEAQVAPVHDPNLRIEFYHNGKPLPSASRFHITFDFGYVSLDITHAVAEDAGEYSVRAVNALGQAVSS-TNLRVIPRGTIISDTQHPE-GLEKIRKLES---TAPHQRQEPETPGTRQRPVFTQPLQNIDRINEHQTAHFEARLIPVGDPNLKVEWYRNEKIIEDSSRITKQHDFGFVSLDISHIRKEDEGVYMCRAVNPLGEAVTTASMRVVS---EASIQMDTQHPDSISRIHQLEKPL--APRPTEPERLFEKPIFTQLLTGPSELW--EGTHAHFEARVVPVGDPSLKFEWFINGVELQMGSRLRTTHDFGFVTLDITAVVPEDAGVYMCRAYNAAGEAVSS-TAMKVKTKSNIDGQPLIPESWEAIRLKEAAMNR-VPEMFVDSTPQQAPVFTTHLQSYDKLHEGQHVLLEAQVEPRADPNLRIEWFKNGISLTTGSRIRSTFDFGLVTLSINGLRADDSAIYTCKATNQVGEAVSTSSLKI-----EDRHWLQAESLHPDS--LPRIGELE 2737 HSP 11 Score: 198.364 bits (503), Expect = 1.599e-51 Identity = 171/634 (26.97%), Postives = 289/634 (45.58%), Query Frame = 3
Query: 168 TMAPHALEVVNQVNKRSLASPRRGSDHLYDSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEE----MMQE--INNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKA-ETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQ----SKGISAIHALEAEL-NAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNIT-AGEGGPAKF---LVKVSGFPTPTLEW 2021
T P ++ ++Q+ K LA + L++ P F Q L + EG RV P D SL+ WF +G L GSR T D GF+ LDI + +D G Y+C A + +G A S+ A +++ +S +D +PE ++E +N + + + T + P F + L + L +GQ + +E +E +D +L EWFK+G+SL GSRI +D G L I +D+ Y+C A N +G+A T S + + + +L S+ ++ LE P E R P FI ++ I E F+ ++P +S +W++NG+ ++ ++ + AL++ + ++G Y C + G + TL K++ LD Q G+ A+ E EL N ++ + E P + K L+ L+E + L VEP D +L++EWY N + +GSR +++ + G T++++ + D G Y A NK G+ + I C E I+ ++ + G ++++DLE+ L Q + P F + +NI GEG A F L+ V G + +EW
Sbjct: 2458 TQHPDSISRIHQLEK-PLAPRPTEPERLFEKPIFTQLLTGPSELWEGTHAHFEARVVPVGDPSLKFEWFINGVELQMGSRLRTTHDFGFVTLDITAVVPEDAGVYMCRAYNAAGEAVSSTA-MKVKTKSNIDGQPLIPESWEAIRLKEAAMNRVPEMFVDST----------PQQAPVFTTHLQSYDKLHEGQHVLLEAQVEPRADPNLRIEWFKNGISLTTGSRIRSTFDFGLVTLSINGLRADDSAIYTCKATNQVGEAVSTSSLKIE---DRHWLQAESLHPDSLPRIGELEAPKE--GRPEAPEPTYETPVFITHLN--NIECKESDNVRFECNVEPARDPTMSIEWFYNGQPLQAAAKFKSIYDFGYCALDLTNSYAENSGVYTCKATNSKGSATTSG-TLKCTGGKTMFLDTQHPQGEAGLEAVQETEEELANRYTSKTTKPETQYPPPVWTKPLQAEFHLSEAQPIHLEANVEPKEDPNLFIEWYFNGKMLNHGSRFKMTSEFGFVTMDMIEVYARDQGIYTCKAYNKAGEAFT-STTIFCSSKENIIESTQHPKGAEGLEQIQDLEDSLRKDGSKPEQPDLGIP----PRFTTEFVNIADIGEGELAHFEANLIPV-GDQSMVIEW 3065 HSP 12 Score: 194.512 bits (493), Expect = 2.508e-50 Identity = 142/516 (27.52%), Postives = 251/516 (48.64%), Query Frame = 3
Query: 243 DHLYDSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQE-INNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTES-----SISQLK-SLENPGEEYARYNDTSVIP---MPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQ----SKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQT 1748
D + P F ++++ L V EG P+ RV P D ++ I WF +G +++ GSR + D GFI LD+ Y D+G+Y C A + GTA ++ A V + + Y +QLPE M E + L++ +EE PKF +Q+ S+T+ + + + E +E +D SL EW+++G L G R I+D G+ +LDI + Y ED+G+Y C A N+ G+ T++ V+C +I+ ++ + +++Q++ +++ E D P PP F+ I +++++E A F+ + P + +W+ NG+ + +R + A+N + P D+G Y+C + +G+ E+ + + V + I+ D Q + I I +EA E+ D P F+ +LEP + EGD V+V V W N + +++GSR +++ D G L++ D+G +IA N G++
Sbjct: 3670 DEQPNPPKFTEEIKDNLDVPEGGPIHFDCRVEPVGDPTMRIEWFYNGHVMATGSRVHQLNDFGFIALDVDYIYARDSGEYTCRATNKWGTATTS-AKVTCKGKHNIVYESQLPEGMTSEKLKELERGRIPEAPKVVEE----VFGPPKFTTQI-TSVTVDEAEAVRFECQVEPKTDPSLRVEWYRNGKPLPSGHRYRNIFDMGFVSLDILYVYGEDSGEYVCRAINNYGEDRTRAT-VSCKKLPTILLQNQVPRGMKRSDALTQMEATIKKYTSEVHLTEDDLFDPDRKQPPRFVTQIK-EQLTLTEMAVTKFECQLAPVGDPNMKVEWFFNGKPLLHKNRFQPIYDFGYVAMNFGWVYPEDSGEYVCRATNLYGKDETRAI-IKVSGKPGIVYDSQLPAHMQSIDRIREMEASWQVVPDEVDPDAKPRTKPVFVSKLEP-QTVEEGDPARFCVRV--TGHPRPRVMWLINGHTVVHGSRYKLTND-GMFHLDVPKTRQYDTGKVEVIARNSVGES 4172 HSP 13 Score: 181.8 bits (460), Expect = 3.302e-46 Identity = 139/515 (26.99%), Postives = 239/515 (46.41%), Query Frame = 3
Query: 258 SPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYA-RYNDTSVIPM----PPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQ----SKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISC 1775
+P F L+S + EG+ + L +V P D +L I WFK+G L+ GSR + FD G + L I DD+ Y C A + G A S + ++I + + P+ + + I L+ E G E P F++ L N++ + + E +E D ++ EWF +G L+ ++ IYD GYCALD+ Y E++G Y+C A NS G A T S + C +++ + + + L++++ EE A RY + P PP + +P+ E +SE P + + ++P L +WY NG+++ SR E ++++ D G Y C +++ G + T+ ++++II Q ++G+ I LE L ++ ++ ++ P F + I + EG+ + P D S+ +EW+ N + R++ Y GT L ++ ED+G Y ATNK+G + ISC
Sbjct: 2621 APVFTTHLQSYDKLHEGQHVLLEAQVEPRADPNLRIEWFKNGISLTTGSRIRSTFDFGLVTLSINGLRADDSAIYTCKATNQVGEAVST-SSLKIEDRHWLQAESLHPDSLPR-IGELEAPKE-----GRPEAPEPTYETPVFITHL-NNIECKESDNVRFECNVEPARDPTMSIEWFYNGQPLQAAAKFKSIYDFGYCALDLTNSYAENSGVYTCKATNSKGSA-TTSGTLKCTGGKTMFLDTQHPQGE-AGLEAVQETEEELANRYTSKTTKPETQYPPPVWTKPLQ-AEFHLSEAQPIHLEANVEPKEDPNLFIEWYFNGKMLNHGSRFKMTSEFGFVTMDMIEVYARDQGIYTCKAYNKAGEAFTS-TTIFCSSKENIIESTQHPKGAEGLEQIQDLEDSLRKDGSKPEQPDLGIP-PRFTTEFVNIADIGEGELAHFEANLIPVGDQSMVIEWFYNGKVLEASHRVRTIYAFGTVALEVLGTKIEDTGTYTCRATNKHGTAE-----ISC 3117 HSP 14 Score: 155.221 bits (391), Expect = 6.442e-38 Identity = 120/399 (30.08%), Postives = 190/399 (47.62%), Query Frame = 3
Query: 144 EGVIPNGTTMAPHALEVVNQVNKRSLASPRRGSDHLYDSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEM-----MQEINNLDKNYETGTYHGMEED--KHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQS-CNVTCHPEESIITKSNLTE--SSISQLKSLENPGEEYARYNDTSVIP-MPPSFIEPIDPREISVSEGAPAYF--KVTIDP---------------GNGQKLSNDWYHNGEIVKTCSRLTGKLEM 1256
E +P G T E + ++ + + + + ++ P F + S + VDE E +R +V P D SL + W+++GK L +G R+ IFD GF+ LDILY +D+G+YVC A ++ G ++ A V + Q+P M + ++ K Y T H E+D K P+F++Q+ LTL + E L D ++ EWF +G L +R IYD GY A++ + YPED+G+Y C A N GK ET++ V+ P I+ S L SI +++ +E + D P P F+ ++P+ +V EG PA F +VT P G+ KL+ND + ++ KT TGK+E+
Sbjct: 3777 ESQLPEGMTS-----EKLKELERGRIPEAPKVVEEVFGPPKFTTQITS-VTVDEAEAVRFECQVEPKTDPSLRVEWYRNGKPLPSGHRYRNIFDMGFVSLDILYVYGEDSGEYVCRAINNYGEDRTR-ATVSCKKLPTILLQNQVPRGMKRSDALTQMEATIKKY-TSEVHLTEDDLFDPDRKQPPRFVTQIKEQLTLTEMAVTKFECQLAPVGDPNMKVEWFFNGKPLLHKNRFQPIYDFGYVAMNFGWVYPEDSGEYVCRATNLYGKDETRAIIKVSGKP--GIVYDSQLPAHMQSIDRIREMEASWQVVPDEVDPDAKPRTKPVFVSKLEPQ--TVEEGDPARFCVRVTGHPRPRVMWLINGHTVVHGSRYKLTNDGMFHLDVPKTRQYDTGKVEV 4163 HSP 15 Score: 145.206 bits (365), Expect = 9.923e-35 Identity = 170/678 (25.07%), Postives = 292/678 (43.07%), Query Frame = 3
Query: 258 SPTFQQDLESELVVDEGEPLRLMTR-VGPADDGSLEINWFKDG-KLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRIN-PESGVDYLTQ-----LPEEMMQ-------EINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQS-CNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIP----MPPSFIEPIDPREI-SVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILD----PQSKGISAIHALE-AELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAG--EGGPAKFLVKVS-----GFPTPTLEWRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVH-VAATNPLGVAKEEATLTV 2189
+P F Q S + V EGEP+ + +G I W KDG ++ S RF I D G L+I +D G Y C A++ +G+ +N A + + P +Y + P ++++ EI L ++ E H ++ P+F+ L N + +G +H+E +E D ++V EW+ +G L +R ++ G+ ALD+ D+G+Y C N+ G AE+++ +V P I +S+ +S+ + LE+ Y+RY T I P FI P+ R++ EG +F+ + P N + +WY +G + SR+T +LNIL+ D G+Y + G S+ ++ V + + D Q + I + LE + + ++E P F ++ L E ++EP D+++ VEW K+ + SR+ ++ G L I D+G Y A N GQ + ++ +IV+ S G Q+++ LE+ G + E+ P F + P+ T EG A F +V G +EW + + + +TY D L+I++ D V+ V A N LG A+++AT+ V
Sbjct: 470 APQFVQRF-STMTVREGEPITMSANAIGTPQP---RITWQKDGVQISSTAERFVGI-DGGATCLEIPRVTANDAGWYQCTAQNIAGST-ANRARLYVEVPREQPNYEQRRLNLPRPTKVIEPEPIPGPEIIYL-RHVERAKPHLRPGEEDRVYPPPQFIIPLQN-VQQTEGGRVHMEARIEPVGDPTMVVEWYLNGRPLAASARATSVFKFGFIALDLLSIMGHDSGEYMCRVTNASGVAESRAILSVVQRPS---IEQSSQNPNSLQYINQLED----YSRYQRTESIDEQLNQAPQFIRPL--RDLGEFEEGKNVHFEAQVTPVNDPSMRVEWYKDGLPITASSRITAIFNFGYVSLNILHLRAEDAGTYTVRAVNRIGEAISQ-SSIRVHSRSQVTADLGIPEQQRYIEKVEELEDYRKSQQRRHVQEAAEAIAPPQFKTPIQNQLDLREHAHAHFEARLEPVGDSTMRVEWLKDGQPLEASSRITTYHNFGYVALTIKQLTIYDAGTYTCRAYNAMGQDTT-VAQLTVISKNEIVSESQ---HPGGLQKIQHLEDSSRYGR----REEEETYITQAPRF-LGPLKGTTKILEGQRAHFEARVEPQSDLGL---VIEWYHNGRSITAANRIQTYYDFGYVALDISQVR-AEDAGVYLVVARNKLGEAQQQATMIV 1116 HSP 16 Score: 118.627 bits (296), Expect = 1.404e-26 Identity = 135/534 (25.28%), Postives = 222/534 (41.57%), Query Frame = 3
Query: 678 PKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEES---------------IITKSNLTESSISQLKSLEN------PGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQS-KGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQE--ECL--PNFDVKPMN-ITAGEGGPAKFLVKVS--GFPTPTLEWRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLTVI 2192
P+F+ + +++T+ +G+P+ + + W KDG+ + + V D G L+I D G Y C A+N G ++ P E +I + I L+ +E PGEE Y PP FI P+ + + +EG + + I+P + +WY NG + +R T + AL++L + D+G Y+C + + G ES + L+V SI Q+ + I+ LE + E DE + P FI+ L + + EG +V +V P ND S+ VEWYK+ I SR+ ++ G +LNI+ +ED+G Y + A N+ G E I+ S ++ + S + AD ++ + +E+ + Q VQE E + P F N + E A F ++ G T +EW D + S TY++ L I + + A N +G A LTVI
Sbjct: 471 PQFVQRF-STMTVREGEPITMSANAIGTPQPRIT--WQKDGVQISSTAERFVGIDGGATCLEIPRVTANDAGWYQCTAQNIAGSTANRARLYVEVPREQPNYEQRRLNLPRPTKVIEPEPIPGPEIIYLRHVERAKPHLRPGEEDRVYP-------PPQFIIPL--QNVQQTEGGRVHMEARIEPVGDPTMVVEWYLNGRPLAASARATSVFKFGFIALDLLSIMGHDSGEYMCRVTNASGVAESRAI-LSVVQRPSIEQSSQNPNSLQYINQLEDYSRYQRTESI-DEQLNQAPQFIRPLRDLGEFEEGKNVHFEAQVTPVNDPSMRVEWYKDGLPITASSRITAIFNFGYVSLNILHLRAEDAGTYTVRAVNRIG----EAISQSSI---RVHSRSQVTADLGIPEQQRYIEKVEELEDYRKSQQRRHVQEAAEAIAPPQFKTPIQNQLDLREHAHAHFEARLEPVGDSTMRVEWLKDGQPLEASSRITTYHNFGYVALTIKQLTIYDAGTYTCRAYNAMGQDTTVAQLTVI 983 HSP 17 Score: 79.337 bits (194), Expect = 1.710e-14 Identity = 105/387 (27.13%), Postives = 161/387 (41.60%), Query Frame = 3
Query: 1083 PPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQL-EPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYD-RGTATLNIVSAISEDSGDYWLIATNKNGQTQSE-KINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVK-PMNITAGEGGPAKFLVKVSGFPTPTLEWRIDD--VLVVPDSVCKTYNDGSINYLEINRC--SELGDIKVHVAATNPLGVAKEEATLTVIPMV---DFR 2210
PP FI+P++P+ E A V +P + S W+ + E +K+ + + + N S L I G + C +E V +V + ++ L PQ + E +P+ F+++L +P++ ++ G+++ LT +V + VEWY N I IS D +G L I E+ G Y +ATNK G++ S+ + I FE + DS L EEDL +S +E P K P I EG AK VKV G P P ++W DD + + + + DG+ + L IN +LG I A NPLGVA A V +V D+R
Sbjct: 6840 PPRFIQPLEPKYFGEHEVAIIEAIVESEPLS----SFQWFVHNEPIKSSNEVRIVSQANKSTLLIENFQSKFVGPFTC---------RAENVGGSVTSTATVNLIPQEEA--------EEFESPR--------------FVEELVQPVEVMD-GEALLLTCQV--TGKPTPKVEWYHNAEKITENKETTISQDLQGVCQLQITEVFPENEGQYECVATNKIGKSVSKTNVKIQAFE---------YIPDSE-ITGLTGSEEDLLDRTLSI--------DEQAPKIIKKLPEKIEPKEGEQAKLEVKVVGKPKPKVKWLRDDEQIFASEEYQIENFEDGT-SVLVINHVYPDDLGTISFE--AYNPLGVAVTTALFAVEGIVGSKDYR 7167 HSP 18 Score: 64.6994 bits (156), Expect = 4.080e-10 Identity = 104/512 (20.31%), Postives = 179/512 (34.96%), Query Frame = 3
Query: 363 INWFKDGKLLSAGSRFSTIFDR--GFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKN---------------------------------------YETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYF--KVTIDPG-------NGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQT 1748
+ W + G L +F ++ G + L I D G+Y C A++ G A I V I PE P +Q I NL+KN YE + E D++ +SQ P S LI+G + + L WF +G L + ++ Y G L + D G Y+ +A N G S + P + + QL++ + +PP+F++ REI +EG F +VT +P NG+++ +D H K +G + I+ + L D G+ C+ ++ G V A+EA+LN +++++++ P F+++ + + EG+ +T++ + W K+ I + + + D G L I + D+G Y A N G T
Sbjct: 118 VTWTRKGAPLLESQKFRMSYNEATGDVSLLINQIGPGDEGEYTCTARNQYGEA---ICSVYIQPEGA-------PMPALQPIQNLEKNIYSNGYSYTSIEEEFRVDTFEYRLLREVSFREAITRRSGYEQDSQLSQELDRNQGPAQAPQISQKPRSSKLIEGSDAVFTARVGSNPKPRLT--WFHNGQRLVASQKYEISYSSGVATLRVKNATARDGGHYTLLAENLQGCV-VSSAVLAVEPAAETAYEPKPVDVMAEQLEAGKA---------------LPPAFVKAFGDREI--TEGRMTRFDCRVTGNPYPEVFWLINGRQVRDDASH-----KILVNESGSHSLMITNVTRL-----DAGAVQCLARNKAGEV----------------------------AIEAQLNV----LEKEQVVA--PQFVQRFSTMT-VREGEPITMSANA--IGTPQPRITWQKDGVQISSTAERFVGIDGGATCLEIPRVTANDAGWYQCTAQNIAGST 552 HSP 19 Score: 62.3882 bits (150), Expect = 2.592e-9 Identity = 101/484 (20.87%), Postives = 174/484 (35.95%), Query Frame = 3
Query: 363 INWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAK---TIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPK--AEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQS------EKINISCFE 1781
+ W+++ + G +L I +D+ + CVA++ GTA+ + V +EE + A K P F++ + S T+ GQ + + L W K+GM ++ + ++ + L I + ED+G Y CVA N+ G+A C+ C I+ S S+ + PG E A P +E + + +V EG+ F+ +D G + W VK + L I P D G+Y CV ++ G ++QT + + P L+AP +K+ + +P+ K T+T KV + V+W++ I Q+ +D +A L I + EDSG + + T+ GQ +S +K IS F+
Sbjct: 4425 VKWYRESFPIQNSPDLQIHTFSGKSILIIRQVFVEDSAVFSCVAENRGGTAKCSANLV------------------------------------VEERRRAGKGGIQPPSFVTTI-QSTTVATGQLARFDAKV--TGTRPLDVYWLKNGMKIQPSIKFKMLEEDSVHTLLIIEPFAEDSGRYECVAVNAAGEA---RCDGDC-----IV-------QSPSKPEKPTTPGSEKA-----------PHIVEQL--KSQTVEEGSKVIFRCRVD-GKPTPTAR-WMRGENFVKPSRYFQMSRQGEYYQLVISEAFPEDEGTYKCVAENKLG---------SIQTSAQLKVRPIEN-----------LDAPPTITALKDVSVTEGMPAQFK-------------TTVTGKV-----KATSVQWFREGQLIPETPDFQMIFDGNSAVLLIGTTYEEDSGIFTVRVTSSTGQVESSAKLTVKKRRISAFQ 4801 HSP 20 Score: 57.7658 bits (138), Expect = 6.737e-8 Identity = 100/456 (21.93%), Postives = 168/456 (36.84%), Query Frame = 3
Query: 726 QPLHIEIFLEHH---------SDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEP-IDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMN-ISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEK-SIILDPQSKGISAI------HALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQI-SYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEWRIDDV 2036
QPL + F EH S+ +WF +K + + ++ L I + G ++C A N +G + T + V P+E EE+ P F+E + P E+ E +VT P +WYHN E + T ++ + L I P + G Y CV ++ G+ S+ NV+ + I D + G++ L + APK II LP +++EP EG+ L VKV V+W +++ I QI +++ GT+ L I +D G A N G + + F E IV S ++++++ L + C P+ + + A G AKF ++ +G P P ++W ++V
Sbjct: 6845 QPLEPKYFGEHEVAIIEAIVESEPLSSFQWFVHNEPIKSSNEVRIVSQANKSTLLIENFQSKFVGPFTCRAEN-VGGSVTSTATVNLIPQEE---------------------AEEFES----------PRFVEELVQPVEVMDGEALLLTCQVTGKPTP----KVEWYHNAEKITENKETTISQDLQGVCQLQITEVFPENEGQYECVATNKIGKSVSK---TNVKIQAFEYIPDSEITGLTGSEEDLLDRTLSIDEQAPK-------IIKKLP---EKIEP----KEGEQAKLEVKV--VGKPKPKVKWLRDDEQIFASEEYQIENFEDGTSVLVINHVYPDDLGTISFEAYNPLGVA----VTTALFAVEGIV-GSKDYRKPEWVSQMEEMQVALKAAK-------------CSPSLLNEMRDCRAALGETAKFSIQFAGNPIPDIQWYFNNV 7227 HSP 21 Score: 55.0694 bits (131), Expect = 3.864e-7 Identity = 77/371 (20.75%), Postives = 150/371 (40.43%), Query Frame = 3
Query: 252 YDSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFD-RGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRIN-----PESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVI-YDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESII-TKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSN-DWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHG 1337
++SP F ++L + V +GE L L +V + ++ W+ + + ++ + D +G L I ++ G Y CVA + G + S +V+I P+S + LT E+++ ++D+ PK + +LP + +G+ +E+ + + +W +D + + ++ G L I YP+D G S A N LG A T + E I+ +K +SQ++ ++ + A+ + PS + + R+ + G A F + G + + WY N ++ + ++ + L I+ D G Y C L +E G
Sbjct: 6939 FESPRFVEELVQPVEVMDGEALLLTCQV--TGKPTPKVEWYHNAEKITENKETTISQDLQGVCQLQITEVFPENEGQYECVATNKIGKSVSK-TNVKIQAFEYIPDSEITGLTGSEEDLLDRTLSIDEQ------------------APKIIKKLPEKIEPKEGEQAKLEVKVVGKPKPKV--KWLRDDEQIFASEEYQIENFEDGTSVLVINHVYPDDLGTISFEAYNPLGVAVTTALFAV----EGIVGSKDYRKPEWVSQMEEMQV-ALKAAKCS--------PSLLNEM--RDCRAALGETAKFSIQF---AGNPIPDIQWYFNNVQLRASEKYRMVVQEQEATLEIMKITSEDCGYYNCKLINEIG 7268 HSP 22 Score: 53.9138 bits (128), Expect = 7.782e-7 Identity = 54/224 (24.11%), Postives = 86/224 (38.39%), Query Frame = 3
Query: 1527 QLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPM-NITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLVVPDSVCKTY-NDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLTVI 2192
+L EG T +V ++ + W+ N ++ + +ISY G ATL + +A + D G Y L+A N G C ++ + + + + E L +G LP VK + EG +F +V+G P P + W I+ V D+ K N+ + L I + L V A N G EA L V+
Sbjct: 265 KLIEGSDAVFTARV--GSNPKPRLTWFHNGQRLVASQKYEISYSSGVATLRVKNATARDGGHYTLLAENLQG----------CVVSSAVLAVEPAAETAYEPKPVDVMAEQLEAGK-------------ALPPAFVKAFGDREITEGRMTRFDCRVTGNPYPEVFWLINGRQVRDDASHKILVNESGSHSLMITNVTRLDAGAVQCLARNKAGEVAIEAQLNVL 463 HSP 23 Score: 51.9878 bits (123), Expect = 3.613e-6 Identity = 83/373 (22.25%), Postives = 144/373 (38.61%), Query Frame = 3
Query: 678 PKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYD-RGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGE-IVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDP---QSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPID-QLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSE-KINISC 1775
P+F+ +L + ++ G+ L + + + EW+ + + + D +G C L I +PE+ G Y CVA N +GK+ +++ NV E I +S I+ L EE S+ P I+ + P +I EG A +V + K+ W + E I + E S L I + P D G+ ++ G VT + + I+ + + +S + ++ L A K PS + ++ L E ++ P D ++WY N + + ++ ATL I+ SED G Y N+ G T + K +IS
Sbjct: 6942 PRFVEELVQPVEVMDGEALLLTCQVTGKPTPKV--EWYHNAEKITENKETTISQDLQGVCQLQITEVFPENEGQYECVATNKIGKSVSKT-NVKIQAFEYI------PDSEITGLTG----SEEDLLDRTLSIDEQAPKIIKKL-PEKIEPKEGEQAKLEVKVVGKPKPKVK--WLRDDEQIFASEEYQIENFEDGTSVLVINHVYPDDLGTISFEAYNPLGVA----VTTALFAVEGIVGSKDYRKPEWVSQMEEMQVALKAAKCS----------PSLLNEMRDCRAALGETAKFSIQFAGNPIPD----IQWYFNNVQLRASEKYRMVVQEQEATLEIMKITSEDCGYYNCKLINEIGMTMTRAKFDISS 7280 HSP 24 Score: 51.2174 bits (121), Expect = 5.794e-6 Identity = 106/518 (20.46%), Postives = 193/518 (37.26%), Query Frame = 3
Query: 651 EDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCA-LDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTI----DPG-----NGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQL--NEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLVVP-DSVCKTYNDGSINYL--EINRCSELGDIKVHVAATNPLG 2159
EDK A P+F+ +L + G + E +E + + WF++ +K + YD A L I +PED G ++ VA+N+ G + + +I +S L++ S +L + +PP+F + P+ V E + + +P NG+ + + N IV + M S ++I S G+Y + + G P+TL V+T + AP+ LEP+ + EG+SV L+ ++ + V WYK+ + N D+ TL +++ + G+Y + A N G ++ TA+ + + AG GN E +E+ +P + G + KVSG P P ++W ++ + P + + + Y+ +I + + N ++ GD K A+NP+G
Sbjct: 14327 EDKPIA---PRFIEKL-QPIHTPDGYTVQFECKVEGNPRPQIA--WFRETAIIKPSQDFQMFYDDDNVATLIIREVFPEDAGQFTVVAKNAAGFTSSTT---------ELIVESPLSDHG-SDATALSRRSMSRESSLADILEGIPPTFSK--KPKAQYVDENTNVILECRLVAVPEPDIVWTFNGEDIDEEEIKNVRIVTESD-----MHMYCSVVHISKVKKSQEGTYEVIATNREGEARL-PITLKVRT--------------------TDKEAPQI-----------------LEPLRNMVIREGESVVLSTQI--VGNPPPKVTWYKDGKPVKNAKS-----DKDLHTLTLITPQKSEKGEYTVKAVNPLGSVET--------------TANLTIEEPAG-------------GNAEPPLFVERFEEQNVP------------QKGEIRLPAKVSGNPVPEVQWLFNNTPLFPSERIQQVYDGENIELIIKDANPETDSGDYK--CIASNPIG 14735
BLAST of Titin vs. Ensembl Fly
Match: sls (gene:FBgn0086906 transcript:FBtr0332677) HSP 1 Score: 258.455 bits (659), Expect = 4.329e-71 Identity = 201/703 (28.59%), Postives = 330/703 (46.94%), Query Frame = 3
Query: 288 ELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGT--YHGMEEDKHAAKTI---------PKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQ-LKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQ-SKGIS---AIHALEAELNAPKAE--MKEDEII----TDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEK-INISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLTVIPMVDFRPDLRHITPENPFK----KMAGLKKVECTSELNDAFKKSK 2315
++V+ E R+ P +D S+ ++WF +GK L AGSR TI D GF++L+I + D+G Y C A + G A + +++ G+ QLP N+ TGT +EE H + + PKF ++ ++L + +G P+H + +E D ++ EWF +G + GSR+ + D G+ ALD+ + Y D+G+Y+C A N G A T S VTC + +I+ +S L E S+ LK LE G + PP F I ++V E F+ ++P L +WY NG+ + + R +M +L+ILY D+G Y+C + +G + T++ + +I+L Q +G+ A+ +EA + +E + ED++ P F+ Q++ L E ++ P D ++ VEW+ N +++ +R Q YD G +N EDSG+Y ATN G+ ++ I +S G IV S L A R++++E AS + + D + P F K T EG PA+F V+V+G P P + W I+ VV S K NDG + +L++ + + KV V A N +G + L V+ D D R++ +P ++A +K +EL F + K
Sbjct: 3547 DMVIGENALAHFECRLQPINDPSMRVDWFHNGKALWAGSRIKTINDFGFVILEIAGCYQRDSGLYTCKATNKHGEATVS-CKLQVKGRQGIVMEPQLP-----------SNFRTGTESLQKLEETMHKREELVTEDEQPNPPKFTEEIKDNLDVPEGGPIHFDCRVEPVGDPTMRIEWFYNGHVMATGSRVHQLNDFGFIALDVDYIYARDSGEYTCRATNKWGTA-TTSAKVTCKGKHNIVYESQLPEGMTSEKLKELER-GRIPEAPKVVEEVFGPPKFTTQIT--SVTVDEAEAVRFECQVEPKTDPSLRVEWYRNGKPLPSGHRYRNIFDMGFVSLDILYVYGEDSGEYVCRAINNYGEDRTR-ATVSCKKLPTILLQNQVPRGMKRSDALTQMEATIKKYTSEVHLTEDDLFDPDRKQPPRFVTQIKEQLTLTEMAVTKFECQLAPVGDPNMKVEWFFNGKPLLHKNRFQPIYDFGYVAMNFGWVYPEDSGEYVCRATNLYGKDETRAIIKVSGKPG--IVYDSQLPAHMQSIDRIREME---ASWQVVPDEVDPDAKPRTKPVFVSKLEPQTVEEGDPARFCVRVTGHPRPRVMWLINGHTVVHGSRYKLTNDG-MFHLDVPKTRQYDTGKVEVIARNSVGESIATTELKVVARSD---DYRNVLKNSPRPWYDYELAAYQKERQENELEKVFDERK 4223 HSP 2 Score: 233.802 bits (595), Expect = 4.669e-63 Identity = 190/684 (27.78%), Postives = 319/684 (46.64%), Query Frame = 3
Query: 297 VDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKA-ETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVT---LNVQTEKSIILDP-QSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSA---GYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAG--------EGGPAKFLVKVSGFPTPTLEWRID-----DVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLTVIPMVDFRPDLRHITPENPFKKMAGLKKVECTS 2285
V E + + R+ P D L + W ++G+ + A +R +T+ D G++ L++ Y +D+G Y C A + G A ++ A + + ++ + TQ E M +I+ L+ + Y EE+++ T P F+++L L++GQ H E +E + D +L EWF +G L G R YD G+ ALDI Y ED+G+Y+C N+LG+A + NVT S I E ++++++ LE+ + ++ P F P+ R V+EGAP + + T+ P N + +WY NG ++T R + AL+ILY DTG+Y+C + G E VT +NV K++ LD ++ + I LE PK ++E P F+ L ++ L EG+ L +VEP ND +L +EW+ N + G R + ++D G L+I+ ED+G Y ATN+ G E +N +C +++ S++ D+ ++++ LE + + + + + P + TA EG A F +V+ P L RI+ L T++ G ++ L+I + V A N LG A L VIP D +H PE + ++K+E T+
Sbjct: 1702 VAENQAVHFEARLIPVGDPKLTVEWLRNGQPIEASNRTTTMHDFGYVALNMKYVNPEDSGTYTCRAVNELGQAVTS-ASLIVQSKTSIQLETQH-EAAMHKIHQLEDH---SRYQRREEEEYTVTTAPVFVTKLIGPSNLVEGQSAHYECRIEPYPDPNLKVEWFHNGKPLSTGHRFRTTYDFGFAALDILTVYAEDSGEYTCRVTNNLGEAINSIVLNVTSR---SSIIHETQHEEALTKIQHLEDTSRFQRKTDEEQFHAERPQFGRPL--RNAKVNEGAPVHLEATLIPVNDPTMKVEWYCNGRPIQTGHRFKTTYDFGFVALDILYAHAEDTGTYMCKAKNAIG----EAVTTCAVNVTANKTLDLDTLDAQRLEKIRQLETYAPPPKPVVEEK---GQKPIFLTPLSNLEHLKEGEHAHLECRVEPINDPNLKIEWFCNGKQLPTGHRYRTTHDFGYVALDILYVYGEDTGTYICKATNQLG----EAVN-TC--NVRVLNRRSMILDTQHPDALEKIQKLESKVPNARTE------------VGDAPISPPHFTAELRGSTEIYEGQTAHFEAQVAPVHDPNL--RIEFYHNGKPLPSASRFHITFDFGYVS-LDITHAVAEDAGEYSVRAVNALGQAVSSTNLRVIPRGTIISDTQH--PEG----LEKIRKLESTA 2340 HSP 3 Score: 219.935 bits (559), Expect = 1.991e-58 Identity = 181/665 (27.22%), Postives = 314/665 (47.22%), Query Frame = 3
Query: 243 DHLYDSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESS--ISQLKSLEN--PGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSK-GISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQE--ECLPNFDVKPMN----ITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLTVIPMVD 2204
D +Y P F L++ + EG + + R+ P D ++ + W+ +G+ L+A +R +++F GFI LD+L + D+G+Y+C + SG A+S A + + ++ +Q P +Q IN L+ +Y Y E P+F+ L + +G+ +H E + +D S+ EW+KDG+ + SRI I++ GY +L+I ED G Y+ A N +G+A +QS ++ H + + E I +++ LE+ ++ + + PP F PI ++ + E A A+F+ ++P + +W +G+ ++ SR+T AL I D G+Y C ++ G+ ++ L V ++ I+ + Q G+ I LE + E +E+ IT P F+ L+ ++ EG +VEP +D L +EWY N I +R+Q YD G L+I +ED+G Y ++A NK G+ Q + I E + SS+ Y++ ++LE N + D++E + P F V P++ I G+ + ++ G PT +EW + V S +TY D L+I + + + V ATN LG A A + VI D
Sbjct: 612 DRVYPPPQFIIPLQN-VQQTEGGRVHMEARIEPVGDPTMVVEWYLNGRPLAASARATSVFKFGFIALDLLSIMGHDSGEYMCRVTNASGVAESR-AILSVVQRPSIEQSSQNPN-SLQYINQLE-DY--SRYQRTESIDEQLNQAPQFIRPLRDLGEFEEGKNVHFEAQVTPVNDPSMRVEWYKDGLPITASSRITAIFNFGYVSLNILHLRAEDAGTYTVRAVNRIGEAISQS-SIRVHSRSQVTADLGIPEQQRYIEKVEELEDYRKSQQRRHVQEAAEAIAPPQFKTPIQ-NQLDLREHAHAHFEARLEPVGDSTMRVEWLKDGQPLEASSRITTYHNFGYVALTIKQLTIYDAGTYTCRAYNAMGQ-DTTVAQLTVISKNEIVSESQHPGGLQKIQHLEDSSRYGRRE-EEETYITQAPRFLGPLKGTTKILEGQRAHFEARVEPQSDLGLVIEWYHNGRSITAANRIQTYYDFGYVALDISQVRAEDAGVYLVVARNKLGEAQQQATMI--VETRSSIDTSSM--HRGLYEKTQNLE------NKPFVEPQYDIEEISKSKPVF-VTPLSDPKPIHDGKNIHLECRLEPMGDPTMRVEWFHNGRPVTVGSRFRTYYDFGFVALDIIKATAADSGEYTVRATNHLGTAHTSACVRVIDHTD 1255 HSP 4 Score: 218.779 bits (556), Expect = 3.771e-58 Identity = 191/640 (29.84%), Postives = 288/640 (45.00%), Query Frame = 3
Query: 339 PADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRIN--PESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTI--PKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNL---TESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQ-SKG----ISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQ-LNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQ-TQSEKINISCFEGEKIVTASSLLAD-SAGYQRLKDLEEDLASGNISTFQNTEDVQ-------EECLPNFDVKPMNITAGEGGPAKFLVKVS--GFPTPTLEWRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLT 2186
P +D L++ W+ +GKL+ +R T+ D G++VLDI Y + D+G+YVC A + G + +N GV Y + P+ +Q I L+ G + D A PKF++Q+ + L++GQ H E L +D LV EW+ +G L G R +D G LDI +CY E++G Y ARN G+ T++ ++ C + S+I S L E + ++ +LE P F P++ E ++ EG A+F+ I P + KL +WY NG +K SR + L I P D+G Y C +E+G + T+ +Q ++SII++ Q KG I I LE L + E D+ P FI P D + E +++P ND S+ V+W+ N + GSR++ D G L I DSG Y ATNK+G+ T S K+ + +G IV L ++ G + L+ LEE + TED Q EE N DV EGGP F +V G PT +EW + ++ S ND L+++ + ATN G A A +T
Sbjct: 3156 PVNDPDLKVEWYHNGKLMRHSNRIKTVSDFGYVVLDISYLQDHDSGEYVCRAWNKYG---EDFTRTTLNCGGRGGVFYDSLQPD-SLQRIRELE------CPQGQQADTSAPLVAEPPKFITQIVDVTKLVEGQSAHFEARLTPITDPDLVVEWYFNGKKLPHGHRFRTFHDFGIVILDILYCYEENSGVYEARARNKYGEDVTRA-SLKCASKSSLILDSQLPRGMEGGLEKIANLEYSMVRTREETTEETKGKAPVFTVPLENIE-NLREGENAHFEARITPADDPKLKVEWYWNGRPLKAGSRFRTFCDFGFVILEISPVYPEDSGEYSCRAINEYGEAVTT-ATMKIQGKRSIIMESQLPKGMEGTIDRIAELEG-LGSRSTEFVPDDDTGKPPEFI--TSPFDMVIGENALAHFECRLQPINDPSMRVDWFHNGKALWAGSRIKTINDFGFVILEIAGCYQRDSGLYTCKATNKHGEATVSCKLQVKGRQG--IVMEPQLPSNFRTGTESLQKLEETMHKREELV---TEDEQPNPPKFTEEIKDNLDV-------PEGGPIHFDCRVEPVGDPTMRIEWFYNGHVMATGSRVHQLNDFGFIALDVDYIYARDSGEYTCRATNKWGTATTSAKVT 3767 HSP 5 Score: 218.779 bits (556), Expect = 4.555e-58 Identity = 174/591 (29.44%), Postives = 279/591 (47.21%), Query Frame = 3
Query: 303 EGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEM---MQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNL---TESSISQLKSLENPGE---EYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQ-----SKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECL--PNFDVKPMNITAGEGGPAKFLVKVSGFPTPTL--EW 2021
EG+ R+ P D L + W+ +GK L G RF T D G ++LDILY E+++G Y A++ G + A ++ +S + +QLP M +++I NL+ + EE K A P F L N L +G+ H E + D L EW+ +G LK GSR D G+ L+I+ YPED+G+YSC A N G+A T + + + SII +S L E +I ++ LE G E+ +DT PP FI P ++ + E A A+F+ + P N + DW+HNG+ + SR+ + L I D+G Y C ++HG + L V+ + I+++PQ G ++ LE ++ + + EDE + P F ++++ + EG + +VEP D ++ +EW+ N + + GSR+ D G L++ + DSG+Y ATNK G T + ++C +G+ + S L + ++LK+LE G I V EE P F + ++T E +F +V P+L EW
Sbjct: 3277 EGQSAHFEARLTPITDPDLVVEWYFNGKKLPHGHRFRTFHDFGIVILDILYCYEENSGVYEARARNKYGEDVTR-ASLKCASKSSLILDSQLPRGMEGGLEKIANLEYSMVRTREETTEETKGKA---PVFTVPLENIENLREGENAHFEARITPADDPKLKVEWYWNGRPLKAGSRFRTFCDFGFVILEISPVYPEDSGEYSCRAINEYGEAVT-TATMKIQGKRSIIMESQLPKGMEGTIDRIAELEGLGSRSTEFVPDDDTG---KPPEFI--TSPFDMVIGENALAHFECRLQPINDPSMRVDWFHNGKALWAGSRIKTINDFGFVILEIAGCYQRDSGLYTCKATNKHGEA-TVSCKLQVKGRQGIVMEPQLPSNFRTGTESLQKLEETMHKREELVTEDE-QPNPPKFTEEIKDNLDVPEGGPIHFDCRVEPVGDPTMRIEWFYNGHVMATGSRVHQLNDFGFIALDVDYIYARDSGEYTCRATNKWG-TATTSAKVTC-KGKHNIVYESQLPEGMTSEKLKELE----RGRIP---EAPKVVEEVFGPPKFTTQITSVTVDEAEAVRFECQVEPKTDPSLRVEW 3846 HSP 6 Score: 212.616 bits (540), Expect = 3.814e-56 Identity = 148/513 (28.85%), Postives = 253/513 (49.32%), Query Frame = 3
Query: 258 SPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTES------SISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQ-SKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISC 1775
+P F LE+ + EGE R+ PADD L++ W+ +G+ L AGSRF T D GF++L+I +D+G+Y C A + G A + A ++I + + +QLP+ M I+ + + G+ P+F++ P + + + H E L+ +D S+ +WF +G +L GSRI I D G+ L+IA CY D+G Y+C A N G+A T SC + + I+ + L + S+ +L+ + EE ++ P PP F E I + V EG P +F ++P + +W++NG ++ T SR+ + AL++ Y D+G Y C ++ G + + + + +I+ + Q +G+++ E E K E + P F Q+ + ++E ++V +VEP D SL VEWY+N + +G R + +D G +L+I+ EDSG+Y A N G+ ++ + +SC
Sbjct: 3400 APVFTVPLENIENLREGENAHFEARITPADDPKLKVEWYWNGRPLKAGSRFRTFCDFGFVILEISPVYPEDSGEYSCRAINEYGEAVTT-ATMKIQGKRSIIMESQLPKGMEGTIDRIAELEGLGSRSTEFVPDDDTGKPPEFITS-PFDMVIGENALAHFECRLQPINDPSMRVDWFHNGKALWAGSRIKTINDFGFVILEIAGCYQRDSGLYTCKATNKHGEA-TVSCKLQVKGRQGIVMEPQLPSNFRTGTESLQKLEETMHKREELVTEDEQ---PNPPKFTEEIKDN-LDVPEGGPIHFDCRVEPVGDPTMRIEWFYNGHVMATGSRVHQLNDFGFIALDVDYIYARDSGEYTCRATNKWGTATTS-AKVTCKGKHNIVYESQLPEGMTSEKLKELERGRIPEAPKVVEEVFGPPKFTTQITSVT-VDEAEAVRFECQVEPKTDPSLRVEWYRNGKPLPSGHRYRNIFDMGFVSLDILYVYGEDSGEYVCRAINNYGEDRT-RATVSC 3902 HSP 7 Score: 206.453 bits (524), Expect = 4.578e-54 Identity = 202/709 (28.49%), Postives = 313/709 (44.15%), Query Frame = 3
Query: 12 HNGAIFKCITRHK--YSFIF-NLDFIAMTTQESMEYNNNSNFNSVPDEGVIPNGTTMAPHALEVVNQV-------NKRSLA-------SPRRGSDH----LYDSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTI-PKFLSQLPNSLT-LIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSK-GISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNI------STFQNTEDVQEE----CLPNFDVK---PMNITAGEGGPAKFLVKVSGFPTPTL--EW 2021
HNG +R + Y F F LD I T +S EY + + GT + V++ N++SL S RR + +P F + L + + EG + L R+ P D S+ I WF +GK + G RF ++ ++ LD+L D+G Y C A++ G A ++ + VRI ++ + TQ E +Q+I L E T H E I P+FL+ P SLT +G H E +E +D +L EWFK+G + +G R I+D GY ALDI ED+G Y+C A N +G ETQ + C E I+T + E+ + Q+ LE+ R P F + + + + E A+F+ + P + + +WYHN +K+ SR T AL+I+ TLP D G+Y C ++ G + V + V T+KSI L+ Q + + + LE + + +DE ++ P F + + ++ E +V ++ P D L VEW +N I +R +D G LN+ EDSG Y A N+ GQ VT++SL+ S +L + + + A I S +Q E+ EE P F K P N+ EG A + ++ +P P L EW
Sbjct: 1196 HNGRPVTVGSRFRTYYDFGFVALDIIKATAADSGEYTVRATNHL---------GTAHTSACVRVIDHTDVVTETQNEQSLEQIQLLEDSRRRHHQEEDITIMQAPQFTRGLHN-IETIEGTNVHLECRLQPVGDPSMRIEWFVNGKPVKTGHRFRPAYEFDYVALDLLGCYAIDSGVYTCQARNQLGEAVTSCS-VRIIAKNDLILETQ-NESGLQKIQYL----EDSTRHRRSEFVDEVVNIRPRFLTH-PKSLTNTREGGHAHFECKIEPVTDPNLKVEWFKNGRPITVGHRFRPIHDFGYVALDIVHLIAEDSGVYTCRAVNLIGSDETQ-VELQCRSGEQIVTVTQ-NEAGLEQIHYLEDRSRYTRREEIDESTKQAPVFTTSL--KNVEIKENQRAHFECRLIPVSDPSMRVEWYHNNLPLKSGSRFTETNNFGFVALDIMSTLPEDAGTYTCRAYNAVGEAITSAVAV-VHTKKSIYLESQHETALPRLQHLEDGSKRQRISV-QDEFVSQAPVFTMPVRDV-RVAENQAVHFEARLIPVGDPKLTVEWLRNGQPIEASNRTTTMHDFGYVALNMKYVNPEDSGTYTCRAVNELGQA---------------VTSASLIVQSKTSIQL-ETQHEAAMHKIHQLEDHSRYQRREE--EEYTVTTAPVFVTKLIGPSNLV--EGQSAHYECRIEPYPDPNLKVEW 1860 HSP 8 Score: 205.682 bits (522), Expect = 8.036e-54 Identity = 162/603 (26.87%), Postives = 284/603 (47.10%), Query Frame = 3
Query: 261 PTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSI-----SQLKSLENPGEEYARYNDTSVIPMPPSFIEPI-DPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQS-KGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADS---AGYQRLKDLEEDLASGNISTFQNTEDVQEECL---PNFDVKPMNIT-AGEGGPAKFLVKVSGFPTPTL--EW 2021
P F+ ++++L + E R+ P D ++ + W KDG+ L A SR +T + G++ L I D G Y C A + G + +A + + ++ + +Q P +Q+I +L+ ++ Y EE++ P+FL L + +++GQ H E +E SD LV EW+ +G S+ +RI YD GY ALDI+ ED G Y VARN LG+A+ Q+ + + T+S++ SS+ + ++LEN +Y+ + P F+ P+ DP+ I +G + + ++P + +W+HNG V SR + AL+I+ +D+G Y V + H + V ++ + Q+ + + I LE + +ED I P F + L I+ + EG +V L +++P D S+ +EW+ N + G R + +Y+ L+++ + DSG Y A N+ G+ + SC +I+ + L+ ++ +G Q+++ LE+ ST + +E + P F P ++T EGG A F K+ P L EW
Sbjct: 890 PQFKTPIQNQLDLREHAHAHFEARLEPVGDSTMRVEWLKDGQPLEASSRITTYHNFGYVALTIKQLTIYDAGTYTCRAYNAMG-QDTTVAQLTVISKNEIVSESQHPG-GLQKIQHLE---DSSRYGRREEEETYITQAPRFLGPLKGTTKILEGQRAHFEARVEPQSDLGLVIEWYHNGRSITAANRIQTYYDFGYVALDISQVRAEDAGVYLVVARNKLGEAQQQATMI-------VETRSSIDTSSMHRGLYEKTQNLENKPFVEPQYDIEEISKSKPVFVTPLSDPKPI--HDGKNIHLECRLEPMGDPTMRVEWFHNGRPVTVGSRFRTYYDFGFVALDIIKATAADSGEY-TVRATNHLGTAHTSACVRVIDHTDVVTETQNEQSLEQIQLLEDSRR--RHHQEEDITIMQAPQFTRGLHNIETI-EGTNVHLECRLQPVGDPSMRIEWFVNGKPVKTGHRFRPAYEFDYVALDLLGCYAIDSGVYTCQARNQLGEAVT-----SC--SVRIIAKNDLILETQNESGLQKIQYLED-------STRHRRSEFVDEVVNIRPRFLTHPKSLTNTREGGHAHFECKIEPVTDPNLKVEW 1460 HSP 9 Score: 203.756 bits (517), Expect = 2.729e-53 Identity = 176/665 (26.47%), Postives = 300/665 (45.11%), Query Frame = 3
Query: 252 YDSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPE--EMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDP-QSKGISAIHALE------AELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADS-------AGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNI-TAGEGGPAKFLVKVSGFPTPTL--EWRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLTV 2189
Y P + + L++E + E +P+ L V P +D +L I W+ +GK+L+ GSRF + GF+ +D++ D G Y C A + +G A ++ + E+ ++ TQ P+ E +++I +L+ + G + ++ P+F ++ N + +G+ H E L D S+V EWF +G L+ R+ IY G AL++ ED G Y+C A N G AE SCN+ C ++ P + R+ S I+P++ + +G A+F+ T+ P N L +WYHNG++++ +R+ + L+I Y D+G Y+C ++++G + TLN + D Q + I LE A+ +AP ++ + P FI Q+ + +L EG S ++ P D L VEWY N + +G R + +D G L+I+ E+SG Y A NK G+ + + ++ C + SSL+ DS G +++ +LE + + T + T + + P F V NI EG A F +++ P L EW + + S +T+ D LEI+ + A N G A AT+ +
Sbjct: 2889 YPPPVWTKPLQAEFHLSEAQPIHLEANVEPKEDPNLFIEWYFNGKMLNHGSRFKMTSEFGFVTMDMIEVYARDQGIYTCKAYNKAGEAFTSTTIFCSSKENIIES-TQHPKGAEGLEQIQDLEDSLRKD---GSKPEQPDLGIPPRFTTEFVNIADIGEGELAHFEANLIPVGDQSMVIEWFYNGKVLEASHRVRTIYAFGTVALEVLGTKIEDTGTYTCRATNKHGTAEI-SCNLEC----------------------VDKPRGQKPRFT---------SHIQPLE----GLKDGQSAHFECTLIPVNDPDLKVEWYHNGKLMRHSNRIKTVSDFGYVVLDISYLQDHDSGEYVCRAWNKYGE-DFTRTTLNCGGRGGVFYDSLQPDSLQRIRELECPQGQQADTSAP--------LVAEPPKFITQIVDVTKLVEGQSAHFEARLTPITDPDLVVEWYFNGKKLPHGHRFRTFHDFGIVILDILYCYEENSGVYEARARNKYGEDVT-RASLKC------ASKSSLILDSQLPRGMEGGLEKIANLEYSM----VRTREETTEETKGKAPVFTVPLENIENLREGENAHFEARITPADDPKLKVEWYWNGRPLKAGSRFRTFCDFGFVILEISPVYPEDSGEYSCRAINEYGEAVTTATMKI 3493 HSP 10 Score: 199.519 bits (506), Expect = 6.112e-52 Identity = 176/599 (29.38%), Postives = 274/599 (45.74%), Query Frame = 3
Query: 75 FIAMTTQESMEYNNNSNFNSVPDEGVIPNGTTMAPHALEVVNQVNKRSLASPRRGSDHLYDSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKA-ETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPID-PREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQ-SKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQ---TQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLE 1853
+I T + E N N + +I + T P ALE + ++ + + D P F +L + EG+ +V P D +L I ++ +GK L + SRF FD G++ LDI + + +D G+Y A + G A S+ ++R+ P + TQ PE +++I L+ T + E + + P F L N + + Q H E L D +L EW+++ ++ SRI +D G+ +LDI+ ED G Y C A N LG+A T S V E+ I SIS++ LE P R + + P F + + P E+ EG A+F+ + P L +W+ NG ++ SRL + L+I +P D G Y+C ++ G S + V+T+ +I P + AI EA +N EM D P F L+ D+L+EG V L +VEP D +L +EW+KN + GSR++ ++D G TL+I ++DS Y ATN+ G+ T S KI E + A SL DS R+ +LE
Sbjct: 2162 YICKATNQLGEAVNTCNVRVLNRRSMILD--TQHPDALEKIQKLESKVPNARTEVGDAPISPPHFTAELRGSTEIYEGQTAHFEAQVAPVHDPNLRIEFYHNGKPLPSASRFHITFDFGYVSLDITHAVAEDAGEYSVRAVNALGQAVSS-TNLRVIPRGTIISDTQHPE-GLEKIRKLES---TAPHQRQEPETPGTRQRPVFTQPLQNIDRINEHQTAHFEARLIPVGDPNLKVEWYRNEKIIEDSSRITKQHDFGFVSLDISHIRKEDEGVYMCRAVNPLGEAVTTASMRVVS---EASIQMDTQHPDSISRIHQLEKPL--APRPTEPERLFEKPIFTQLLTGPSELW--EGTHAHFEARVVPVGDPSLKFEWFINGVELQMGSRLRTTHDFGFVTLDITAVVPEDAGVYMCRAYNAAGEAVSS-TAMKVKTKSNIDGQPLIPESWEAIRLKEAAMNR-VPEMFVDSTPQQAPVFTTHLQSYDKLHEGQHVLLEAQVEPRADPNLRIEWFKNGISLTTGSRIRSTFDFGLVTLSINGLRADDSAIYTCKATNQVGEAVSTSSLKI-----EDRHWLQAESLHPDS--LPRIGELE 2737 HSP 11 Score: 197.593 bits (501), Expect = 2.698e-51 Identity = 171/634 (26.97%), Postives = 289/634 (45.58%), Query Frame = 3
Query: 168 TMAPHALEVVNQVNKRSLASPRRGSDHLYDSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEE----MMQE--INNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKA-ETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQ----SKGISAIHALEAEL-NAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNIT-AGEGGPAKF---LVKVSGFPTPTLEW 2021
T P ++ ++Q+ K LA + L++ P F Q L + EG RV P D SL+ WF +G L GSR T D GF+ LDI + +D G Y+C A + +G A S+ A +++ +S +D +PE ++E +N + + + T + P F + L + L +GQ + +E +E +D +L EWFK+G+SL GSRI +D G L I +D+ Y+C A N +G+A T S + + + +L S+ ++ LE P E R P FI ++ I E F+ ++P +S +W++NG+ ++ ++ + AL++ + ++G Y C + G + TL K++ LD Q G+ A+ E EL N ++ + E P + K L+ L+E + L VEP D +L++EWY N + +GSR +++ + G T++++ + D G Y A NK G+ + I C E I+ ++ + G ++++DLE+ L Q + P F + +NI GEG A F L+ V G + +EW
Sbjct: 2458 TQHPDSISRIHQLEK-PLAPRPTEPERLFEKPIFTQLLTGPSELWEGTHAHFEARVVPVGDPSLKFEWFINGVELQMGSRLRTTHDFGFVTLDITAVVPEDAGVYMCRAYNAAGEAVSSTA-MKVKTKSNIDGQPLIPESWEAIRLKEAAMNRVPEMFVDST----------PQQAPVFTTHLQSYDKLHEGQHVLLEAQVEPRADPNLRIEWFKNGISLTTGSRIRSTFDFGLVTLSINGLRADDSAIYTCKATNQVGEAVSTSSLKIE---DRHWLQAESLHPDSLPRIGELEAPKE--GRPEAPEPTYETPVFITHLN--NIECKESDNVRFECNVEPARDPTMSIEWFYNGQPLQAAAKFKSIYDFGYCALDLTNSYAENSGVYTCKATNSKGSATTSG-TLKCTGGKTMFLDTQHPQGEAGLEAVQETEEELANRYTSKTTKPETQYPPPVWTKPLQAEFHLSEAQPIHLEANVEPKEDPNLFIEWYFNGKMLNHGSRFKMTSEFGFVTMDMIEVYARDQGIYTCKAYNKAGEAFT-STTIFCSSKENIIESTQHPKGAEGLEQIQDLEDSLRKDGSKPEQPDLGIP----PRFTTEFVNIADIGEGELAHFEANLIPV-GDQSMVIEW 3065 HSP 12 Score: 194.512 bits (493), Expect = 2.460e-50 Identity = 142/516 (27.52%), Postives = 251/516 (48.64%), Query Frame = 3
Query: 243 DHLYDSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQE-INNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTES-----SISQLK-SLENPGEEYARYNDTSVIP---MPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQ----SKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQT 1748
D + P F ++++ L V EG P+ RV P D ++ I WF +G +++ GSR + D GFI LD+ Y D+G+Y C A + GTA ++ A V + + Y +QLPE M E + L++ +EE PKF +Q+ S+T+ + + + E +E +D SL EW+++G L G R I+D G+ +LDI + Y ED+G+Y C A N+ G+ T++ V+C +I+ ++ + +++Q++ +++ E D P PP F+ I +++++E A F+ + P + +W+ NG+ + +R + A+N + P D+G Y+C + +G+ E+ + + V + I+ D Q + I I +EA E+ D P F+ +LEP + EGD V+V V W N + +++GSR +++ D G L++ D+G +IA N G++
Sbjct: 3670 DEQPNPPKFTEEIKDNLDVPEGGPIHFDCRVEPVGDPTMRIEWFYNGHVMATGSRVHQLNDFGFIALDVDYIYARDSGEYTCRATNKWGTATTS-AKVTCKGKHNIVYESQLPEGMTSEKLKELERGRIPEAPKVVEE----VFGPPKFTTQI-TSVTVDEAEAVRFECQVEPKTDPSLRVEWYRNGKPLPSGHRYRNIFDMGFVSLDILYVYGEDSGEYVCRAINNYGEDRTRAT-VSCKKLPTILLQNQVPRGMKRSDALTQMEATIKKYTSEVHLTEDDLFDPDRKQPPRFVTQIK-EQLTLTEMAVTKFECQLAPVGDPNMKVEWFFNGKPLLHKNRFQPIYDFGYVAMNFGWVYPEDSGEYVCRATNLYGKDETRAI-IKVSGKPGIVYDSQLPAHMQSIDRIREMEASWQVVPDEVDPDAKPRTKPVFVSKLEP-QTVEEGDPARFCVRV--TGHPRPRVMWLINGHTVVHGSRYKLTND-GMFHLDVPKTRQYDTGKVEVIARNSVGES 4172 HSP 13 Score: 181.415 bits (459), Expect = 4.331e-46 Identity = 139/515 (26.99%), Postives = 239/515 (46.41%), Query Frame = 3
Query: 258 SPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYA-RYNDTSVIPM----PPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQ----SKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISC 1775
+P F L+S + EG+ + L +V P D +L I WFK+G L+ GSR + FD G + L I DD+ Y C A + G A S + ++I + + P+ + + I L+ E G E P F++ L N++ + + E +E D ++ EWF +G L+ ++ IYD GYCALD+ Y E++G Y+C A NS G A T S + C +++ + + + L++++ EE A RY + P PP + +P+ E +SE P + + ++P L +WY NG+++ SR E ++++ D G Y C +++ G + T+ ++++II Q ++G+ I LE L ++ ++ ++ P F + I + EG+ + P D S+ +EW+ N + R++ Y GT L ++ ED+G Y ATNK+G + ISC
Sbjct: 2621 APVFTTHLQSYDKLHEGQHVLLEAQVEPRADPNLRIEWFKNGISLTTGSRIRSTFDFGLVTLSINGLRADDSAIYTCKATNQVGEAVST-SSLKIEDRHWLQAESLHPDSLPR-IGELEAPKE-----GRPEAPEPTYETPVFITHL-NNIECKESDNVRFECNVEPARDPTMSIEWFYNGQPLQAAAKFKSIYDFGYCALDLTNSYAENSGVYTCKATNSKGSA-TTSGTLKCTGGKTMFLDTQHPQGE-AGLEAVQETEEELANRYTSKTTKPETQYPPPVWTKPLQA-EFHLSEAQPIHLEANVEPKEDPNLFIEWYFNGKMLNHGSRFKMTSEFGFVTMDMIEVYARDQGIYTCKAYNKAGEAFTS-TTIFCSSKENIIESTQHPKGAEGLEQIQDLEDSLRKDGSKPEQPDLGIP-PRFTTEFVNIADIGEGELAHFEANLIPVGDQSMVIEWFYNGKVLEASHRVRTIYAFGTVALEVLGTKIEDTGTYTCRATNKHGTAE-----ISC 3117 HSP 14 Score: 155.221 bits (391), Expect = 6.603e-38 Identity = 120/399 (30.08%), Postives = 190/399 (47.62%), Query Frame = 3
Query: 144 EGVIPNGTTMAPHALEVVNQVNKRSLASPRRGSDHLYDSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEM-----MQEINNLDKNYETGTYHGMEED--KHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQS-CNVTCHPEESIITKSNLTE--SSISQLKSLENPGEEYARYNDTSVIP-MPPSFIEPIDPREISVSEGAPAYF--KVTIDP---------------GNGQKLSNDWYHNGEIVKTCSRLTGKLEM 1256
E +P G T E + ++ + + + + ++ P F + S + VDE E +R +V P D SL + W+++GK L +G R+ IFD GF+ LDILY +D+G+YVC A ++ G ++ A V + Q+P M + ++ K Y T H E+D K P+F++Q+ LTL + E L D ++ EWF +G L +R IYD GY A++ + YPED+G+Y C A N GK ET++ V+ P I+ S L SI +++ +E + D P P F+ ++P+ +V EG PA F +VT P G+ KL+ND + ++ KT TGK+E+
Sbjct: 3777 ESQLPEGMTS-----EKLKELERGRIPEAPKVVEEVFGPPKFTTQITS-VTVDEAEAVRFECQVEPKTDPSLRVEWYRNGKPLPSGHRYRNIFDMGFVSLDILYVYGEDSGEYVCRAINNYGEDRTR-ATVSCKKLPTILLQNQVPRGMKRSDALTQMEATIKKY-TSEVHLTEDDLFDPDRKQPPRFVTQIKEQLTLTEMAVTKFECQLAPVGDPNMKVEWFFNGKPLLHKNRFQPIYDFGYVAMNFGWVYPEDSGEYVCRATNLYGKDETRAIIKVSGKP--GIVYDSQLPAHMQSIDRIREMEASWQVVPDEVDPDAKPRTKPVFVSKLEPQ--TVEEGDPARFCVRVTGHPRPRVMWLINGHTVVHGSRYKLTNDGMFHLDVPKTRQYDTGKVEV 4163 HSP 15 Score: 144.436 bits (363), Expect = 1.447e-34 Identity = 170/678 (25.07%), Postives = 292/678 (43.07%), Query Frame = 3
Query: 258 SPTFQQDLESELVVDEGEPLRLMTR-VGPADDGSLEINWFKDG-KLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRIN-PESGVDYLTQ-----LPEEMMQ-------EINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQS-CNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIP----MPPSFIEPIDPREI-SVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILD----PQSKGISAIHALE-AELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAG--EGGPAKFLVKVS-----GFPTPTLEWRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVH-VAATNPLGVAKEEATLTV 2189
+P F Q S + V EGEP+ + +G I W KDG ++ S RF I D G L+I +D G Y C A++ +G+ +N A + + P +Y + P ++++ EI L ++ E H ++ P+F+ L N + +G +H+E +E D ++V EW+ +G L +R ++ G+ ALD+ D+G+Y C N+ G AE+++ +V P I +S+ +S+ + LE+ Y+RY T I P FI P+ R++ EG +F+ + P N + +WY +G + SR+T +LNIL+ D G+Y + G S+ ++ V + + D Q + I + LE + + ++E P F ++ L E ++EP D+++ VEW K+ + SR+ ++ G L I D+G Y A N GQ + ++ +IV+ S G Q+++ LE+ G + E+ P F + P+ T EG A F +V G +EW + + + +TY D L+I++ D V+ V A N LG A+++AT+ V
Sbjct: 470 APQFVQRF-STMTVREGEPITMSANAIGTPQP---RITWQKDGVQISSTAERFVGI-DGGATCLEIPRVTANDAGWYQCTAQNIAGST-ANRARLYVEVPREQPNYEQRRLNLPRPTKVIEPEPIPGPEIIYL-RHVERAKPHLRPGEEDRVYPPPQFIIPLQN-VQQTEGGRVHMEARIEPVGDPTMVVEWYLNGRPLAASARATSVFKFGFIALDLLSIMGHDSGEYMCRVTNASGVAESRAILSVVQRPS---IEQSSQNPNSLQYINQLED----YSRYQRTESIDEQLNQAPQFIRPL--RDLGEFEEGKNVHFEAQVTPVNDPSMRVEWYKDGLPITASSRITAIFNFGYVSLNILHLRAEDAGTYTVRAVNRIGEAISQS-SIRVHSRSQVTADLGIPEQQRYIEKVEELEDYRKSQQRRHVQEAAEAIAPPQFKTPIQNQLDLREHAHAHFEARLEPVGDSTMRVEWLKDGQPLEASSRITTYHNFGYVALTIKQLTIYDAGTYTCRAYNAMGQDTT-VAQLTVISKNEIVSESQ---HPGGLQKIQHLEDSSRYGR----REEEETYITQAPRF-LGPLKGTTKILEGQRAHFEARVEPQSDLGL---VIEWYHNGRSITAANRIQTYYDFGYVALDISQVR-AEDAGVYLVVARNKLGEAQQQATMIV 1116 HSP 16 Score: 118.242 bits (295), Expect = 1.989e-26 Identity = 135/534 (25.28%), Postives = 222/534 (41.57%), Query Frame = 3
Query: 678 PKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEES---------------IITKSNLTESSISQLKSLEN------PGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQS-KGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQE--ECL--PNFDVKPMN-ITAGEGGPAKFLVKVS--GFPTPTLEWRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLTVI 2192
P+F+ + +++T+ +G+P+ + + W KDG+ + + V D G L+I D G Y C A+N G ++ P E +I + I L+ +E PGEE Y PP FI P+ + + +EG + + I+P + +WY NG + +R T + AL++L + D+G Y+C + + G ES + L+V SI Q+ + I+ LE + E DE + P FI+ L + + EG +V +V P ND S+ VEWYK+ I SR+ ++ G +LNI+ +ED+G Y + A N+ G E I+ S ++ + S + AD ++ + +E+ + Q VQE E + P F N + E A F ++ G T +EW D + S TY++ L I + + A N +G A LTVI
Sbjct: 471 PQFVQRF-STMTVREGEPITMSANAIGTPQPRIT--WQKDGVQISSTAERFVGIDGGATCLEIPRVTANDAGWYQCTAQNIAGSTANRARLYVEVPREQPNYEQRRLNLPRPTKVIEPEPIPGPEIIYLRHVERAKPHLRPGEEDRVYP-------PPQFIIPL--QNVQQTEGGRVHMEARIEPVGDPTMVVEWYLNGRPLAASARATSVFKFGFIALDLLSIMGHDSGEYMCRVTNASGVAESRAI-LSVVQRPSIEQSSQNPNSLQYINQLEDYSRYQRTESI-DEQLNQAPQFIRPLRDLGEFEEGKNVHFEAQVTPVNDPSMRVEWYKDGLPITASSRITAIFNFGYVSLNILHLRAEDAGTYTVRAVNRIG----EAISQSSI---RVHSRSQVTADLGIPEQQRYIEKVEELEDYRKSQQRRHVQEAAEAIAPPQFKTPIQNQLDLREHAHAHFEARLEPVGDSTMRVEWLKDGQPLEASSRITTYHNFGYVALTIKQLTIYDAGTYTCRAYNAMGQDTTVAQLTVI 983 HSP 17 Score: 78.9518 bits (193), Expect = 2.058e-14 Identity = 105/387 (27.13%), Postives = 161/387 (41.60%), Query Frame = 3
Query: 1083 PPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQL-EPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYD-RGTATLNIVSAISEDSGDYWLIATNKNGQTQSE-KINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVK-PMNITAGEGGPAKFLVKVSGFPTPTLEWRIDD--VLVVPDSVCKTYNDGSINYLEINRC--SELGDIKVHVAATNPLGVAKEEATLTVIPMV---DFR 2210
PP FI+P++P+ E A V +P + S W+ + E +K+ + + + N S L I G + C +E V +V + ++ L PQ + E +P+ F+++L +P++ ++ G+++ LT +V + VEWY N I IS D +G L I E+ G Y +ATNK G++ S+ + I FE + DS L EEDL +S +E P K P I EG AK VKV G P P ++W DD + + + + DG+ + L IN +LG I A NPLGVA A V +V D+R
Sbjct: 6840 PPRFIQPLEPKYFGEHEVAIIEAIVESEPLS----SFQWFVHNEPIKSSNEVRIVSQANKSTLLIENFQSKFVGPFTC---------RAENVGGSVTSTATVNLIPQEEA--------EEFESPR--------------FVEELVQPVEVMD-GEALLLTCQV--TGKPTPKVEWYHNAEKITENKETTISQDLQGVCQLQITEVFPENEGQYECVATNKIGKSVSKTNVKIQAFE---------YIPDSE-ITGLTGSEEDLLDRTLSI--------DEQAPKIIKKLPEKIEPKEGEQAKLEVKVVGKPKPKVKWLRDDEQIFASEEYQIENFEDGT-SVLVINHVYPDDLGTISFE--AYNPLGVAVTTALFAVEGIVGSKDYR 7167 HSP 18 Score: 65.0846 bits (157), Expect = 3.654e-10 Identity = 104/512 (20.31%), Postives = 182/512 (35.55%), Query Frame = 3
Query: 363 INWFKDGKLLSAGSRFSTIFDR--GFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYH-------------------------------GMEEDKHAAKTIPK--------FLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYF--KVTIDPG-------NGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQT 1748
+ W + G L +F ++ G + L I D G+Y C A++ G A I V I PE P +Q I NL+KN + Y G E+D ++ + + +SQ P S LI+G + + L WF +G L + ++ Y G L + D G Y+ +A N G S + P + + QL++ + +PP+F++ REI +EG F +VT +P NG+++ +D H K +G + I+ + L D G+ C+ ++ G V A+EA+LN +++++++ P F+++ + + EG+ +T++ + W K+ I + + + D G L I + D+G Y A N G T
Sbjct: 118 VTWTRKGAPLLESQKFRMSYNEATGDVSLLINQIGPGDEGEYTCTARNQYGEA---ICSVYIQPEGA-------PMPALQPIQNLEKNIYSNGYSYTSIEEEFRVDTFEYRLLREVSFREAITRRSGYEQDSQLSQELDRNQGPAQAPQISQKPRSSKLIEGSDAVFTARVGSNPKPRLT--WFHNGQRLVASQKYEISYSSGVATLRVKNATARDGGHYTLLAENLQGCV-VSSAVLAVEPAAETAYEPKPVDVMAEQLEAGKA---------------LPPAFVKAFGDREI--TEGRMTRFDCRVTGNPYPEVFWLINGRQVRDDASH-----KILVNESGSHSLMITNVTRL-----DAGAVQCLARNKAGEV----------------------------AIEAQLNV----LEKEQVVA--PQFVQRFSTMT-VREGEPITMSANA--IGTPQPRITWQKDGVQISSTAERFVGIDGGATCLEIPRVTANDAGWYQCTAQNIAGST 552 HSP 19 Score: 62.3882 bits (150), Expect = 2.484e-9 Identity = 102/485 (21.03%), Postives = 176/485 (36.29%), Query Frame = 3
Query: 363 INWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAK---TIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGE-IVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPK--AEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQS------EKINISCFE 1781
+ W+++ + G +L I +D+ + CVA++ GTA+ + V +EE + A K P F++ + S T+ GQ + + L W K+GM ++ + ++ + L I + ED+G Y CVA N+ G+A C+ C I+ S S+ + PG E A P +E + + +V EG+ F+ +D G+ + GE VK + L I P D G+Y CV ++ G ++QT + + P L+AP +K+ + +P+ K T+T KV + V+W++ I Q+ +D +A L I + EDSG + + T+ GQ +S +K IS F+
Sbjct: 4425 VKWYRESFPIQNSPDLQIHTFSGKSILIIRQVFVEDSAVFSCVAENRGGTAKCSANLV------------------------------------VEERRRAGKGGIQPPSFVTTI-QSTTVATGQLARFDAKV--TGTRPLDVYWLKNGMKIQPSIKFKMLEEDSVHTLLIIEPFAEDSGRYECVAVNAAGEA---RCDGDC-----IV-------QSPSKPEKPTTPGSEKA-----------PHIVEQLKSQ--TVEEGSKVIFRCRVD---GKPTPTARWMRGENFVKPSRYFQMSRQGEYYQLVISEAFPEDEGTYKCVAENKLG---------SIQTSAQLKVRPIEN-----------LDAPPTITALKDVSVTEGMPAQFK-------------TTVTGKV-----KATSVQWFREGQLIPETPDFQMIFDGNSAVLLIGTTYEEDSGIFTVRVTSSTGQVESSAKLTVKKRRISAFQ 4801 HSP 20 Score: 57.3806 bits (137), Expect = 8.108e-8 Identity = 98/443 (22.12%), Postives = 165/443 (37.25%), Query Frame = 3
Query: 738 IEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEP-IDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMN-ISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEK-SIILDPQSKGISAI------HALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQI-SYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEWRIDDV 2036
IE +E SS +WF +K + + ++ L I + G ++C A N +G + T + V P+E EE+ P F+E + P E+ E +VT P +WYHN E + T ++ + L I P + G Y CV ++ G+ S+ NV+ + I D + G++ L + APK II LP +++EP EG+ L VKV V+W +++ I QI +++ GT+ L I +D G A N G + + F E IV S ++++++ L + C P+ + + A G AKF ++ +G P P ++W ++V
Sbjct: 6860 IEAIVESEPLSSF--QWFVHNEPIKSSNEVRIVSQANKSTLLIENFQSKFVGPFTCRAEN-VGGSVTSTATVNLIPQEE---------------------AEEFES----------PRFVEELVQPVEVMDGEALLLTCQVTGKPTP----KVEWYHNAEKITENKETTISQDLQGVCQLQITEVFPENEGQYECVATNKIGKSVSK---TNVKIQAFEYIPDSEITGLTGSEEDLLDRTLSIDEQAPK-------IIKKLP---EKIEP----KEGEQAKLEVKV--VGKPKPKVKWLRDDEQIFASEEYQIENFEDGTSVLVINHVYPDDLGTISFEAYNPLGVA----VTTALFAVEGIV-GSKDYRKPEWVSQMEEMQVALKAAK-------------CSPSLLNEMRDCRAALGETAKFSIQFAGNPIPDIQWYFNNV 7227 HSP 21 Score: 55.0694 bits (131), Expect = 3.461e-7 Identity = 77/371 (20.75%), Postives = 150/371 (40.43%), Query Frame = 3
Query: 252 YDSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFD-RGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRIN-----PESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVI-YDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESII-TKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSN-DWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHG 1337
++SP F ++L + V +GE L L +V + ++ W+ + + ++ + D +G L I ++ G Y CVA + G + S +V+I P+S + LT E+++ ++D+ PK + +LP + +G+ +E+ + + +W +D + + ++ G L I YP+D G S A N LG A T + E I+ +K +SQ++ ++ + A+ + PS + + R+ + G A F + G + + WY N ++ + ++ + L I+ D G Y C L +E G
Sbjct: 6939 FESPRFVEELVQPVEVMDGEALLLTCQV--TGKPTPKVEWYHNAEKITENKETTISQDLQGVCQLQITEVFPENEGQYECVATNKIGKSVSK-TNVKIQAFEYIPDSEITGLTGSEEDLLDRTLSIDEQ------------------APKIIKKLPEKIEPKEGEQAKLEVKVVGKPKPKV--KWLRDDEQIFASEEYQIENFEDGTSVLVINHVYPDDLGTISFEAYNPLGVAVTTALFAV----EGIVGSKDYRKPEWVSQMEEMQV-ALKAAKCS--------PSLLNEM--RDCRAALGETAKFSIQF---AGNPIPDIQWYFNNVQLRASEKYRMVVQEQEATLEIMKITSEDCGYYNCKLINEIG 7268 HSP 22 Score: 53.5286 bits (127), Expect = 1.054e-6 Identity = 54/224 (24.11%), Postives = 86/224 (38.39%), Query Frame = 3
Query: 1527 QLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPM-NITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLVVPDSVCKTY-NDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLTVI 2192
+L EG T +V ++ + W+ N ++ + +ISY G ATL + +A + D G Y L+A N G C ++ + + + + E L +G LP VK + EG +F +V+G P P + W I+ V D+ K N+ + L I + L V A N G EA L V+
Sbjct: 265 KLIEGSDAVFTARV--GSNPKPRLTWFHNGQRLVASQKYEISYSSGVATLRVKNATARDGGHYTLLAENLQG----------CVVSSAVLAVEPAAETAYEPKPVDVMAEQLEAGK-------------ALPPAFVKAFGDREITEGRMTRFDCRVTGNPYPEVFWLINGRQVRDDASHKILVNESGSHSLMITNVTRLDAGAVQCLARNKAGEVAIEAQLNVL 463 HSP 23 Score: 51.9878 bits (123), Expect = 3.673e-6 Identity = 83/372 (22.31%), Postives = 144/372 (38.71%), Query Frame = 3
Query: 678 PKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYD-RGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGE-IVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDP---QSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPID-QLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSE-KINIS 1772
P+F+ +L + ++ G+ L + + + EW+ + + + D +G C L I +PE+ G Y CVA N +GK+ +++ NV E I +S I+ L EE S+ P I+ + P +I EG A +V + K+ W + E I + E S L I + P D G+ ++ G VT + + I+ + + +S + ++ L A K PS + ++ L E ++ P D ++WY N + + ++ ATL I+ SED G Y N+ G T + K +IS
Sbjct: 6942 PRFVEELVQPVEVMDGEALLLTCQVTGKPTPKV--EWYHNAEKITENKETTISQDLQGVCQLQITEVFPENEGQYECVATNKIGKSVSKT-NVKIQAFEYI------PDSEITGLTG----SEEDLLDRTLSIDEQAPKIIKKL-PEKIEPKEGEQAKLEVKVVGKPKPKVK--WLRDDEQIFASEEYQIENFEDGTSVLVINHVYPDDLGTISFEAYNPLGVA----VTTALFAVEGIVGSKDYRKPEWVSQMEEMQVALKAAKCS----------PSLLNEMRDCRAALGETAKFSIQFAGNPIPD----IQWYFNNVQLRASEKYRMVVQEQEATLEIMKITSEDCGYYNCKLINEIGMTMTRAKFDIS 7279 HSP 24 Score: 50.8322 bits (120), Expect = 6.741e-6 Identity = 106/518 (20.46%), Postives = 193/518 (37.26%), Query Frame = 3
Query: 651 EDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCA-LDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTI----DPG-----NGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQL--NEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLVVP-DSVCKTYNDGSINYL--EINRCSELGDIKVHVAATNPLG 2159
EDK A P+F+ +L + G + E +E + + WF++ +K + YD A L I +PED G ++ VA+N+ G + + +I +S L++ S +L + +PP+F + P+ V E + + +P NG+ + + N IV + M S ++I S G+Y + + G P+TL V+T + AP+ LEP+ + EG+SV L+ ++ + V WYK+ + N D+ TL +++ + G+Y + A N G ++ TA+ + + AG GN E +E+ +P + G + KVSG P P ++W ++ + P + + + Y+ +I + + N ++ GD K A+NP+G
Sbjct: 13855 EDKPIA---PRFIEKL-QPIHTPDGYTVQFECKVEGNPRPQIA--WFRETAIIKPSQDFQMFYDDDNVATLIIREVFPEDAGQFTVVAKNAAGFTSSTT---------ELIVESPLSDHG-SDATALSRRSMSRESSLADILEGIPPTFSK--KPKAQYVDENTNVILECRLVAVPEPDIVWTFNGEDIDEEEIKNVRIVTESD-----MHMYCSVVHISKVKKSQEGTYEVIATNREGEARL-PITLKVRT--------------------TDKEAPQI-----------------LEPLRNMVIREGESVVLSTQI--VGNPPPKVTWYKDGKPVKNAKS-----DKDLHTLTLITPQKSEKGEYTVKAVNPLGSVET--------------TANLTIEEPAG-------------GNAEPPLFVERFEEQNVP------------QKGEIRLPAKVSGNPVPEVQWLFNNTPLFPSERIQQVYDGENIELIIKDANPETDSGDYK--CIASNPIG 14263
BLAST of Titin vs. Ensembl Zebrafish
Match: ttn.2 (titin, tandem duplicate 2 [Source:ZFIN;Acc:ZDB-GENE-030113-2]) HSP 1 Score: 119.783 bits (299), Expect = 9.638e-27 Identity = 155/672 (23.07%), Postives = 265/672 (39.43%), Query Frame = 3
Query: 360 EINWFKDGKLLSAGSRFST-IFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLD------KNYETGTYHGMEEDKHAAKTI-------------PKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSL--KLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSN-DWYHNGEIVKTCSRLTGKLE--MNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTE-------KSIIL--------DPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLTVIPMVDFRPDLRHI----TPENP 2243
+I W+KDGK + G R+ + G L + + +D G Y A + G A S+ + + Y Q E ++ + + +Y G + A+ + P F+ Q P+S +GQ ++ + T WF G + +I V+ + G ++ + P+D+G+++ VA+N GK T S +T +E+++ P FIE + + ISV EG+ V G L + W N +I+ K+E + I T SD+ Y ++ GR ++ +NV+ E + +I+ D I +H + + ++ + E P F K+L + ++ V ++ P D ++ VEW + + +R+++ + G +L+ A S DSG ATNK G Q+ I + EK + S L + QR+ ++E G S +D+ E+ P + P I EG AK+ +V+G+PTP + W ++ +L+ + + DG I+YLEI C V V A NP G A+ L + DFR LR PE P
Sbjct: 756 KIAWYKDGKRIKHGGRYQMEVLQDGRASLRLPVVLPEDEGIYTAFASNMKGNAVSSGKLYVESTAAAQPYYPQA--EAVRRVQSTSPMSARSASYSPGRSPARSVSRSPARRLDDTDESQLERLYKPVFV-QKPSSFRCSEGQTARFDLKVVGRPMPD--TFWFHSGQQVVNDYTHKI-VVKEDGTQSMIVVPAMPQDSGEWTVVAQNRAGKT-TVSMTLTVEAKENLVR----------------------------------PQFIEKL--KNISVKEGSLVELAVK---AIGNPLPDIVWLKNSDIISPHKYPHIKIEGAKGQAHFQIPQTSGSDSAWYTATAINKAGR-DTTRCRVNVEVEYTEPVPERRLIIPKGTYKAKDVAPPEIEPLHLRYGQEQWEEGDLYDKEK-QQKPHFKKKLTSV-RMKRFGPVHFECRLTPIGDPTMVVEWLHDGKPLEAANRLRMINEFGYCSLDYEVAYSRDSGVITCRATNKFGADQTSATLI--VKDEKSLVEDSQLPEGRKVQRMDEMERIAHEGGPSGVTG-DDMSEKSKPEIVLLPEPIRVFEGETAKYRCRVTGYPTPKVNWYLNGLLIRKSKRFRLHYDG-IHYLEITDCKSYDSGDVRVLAENPEGTAEHTVKLEIQQKEDFRTILRRAPELKAPEAP 1374 HSP 2 Score: 78.1814 bits (191), Expect = 4.279e-14 Identity = 108/463 (23.33%), Postives = 182/463 (39.31%), Query Frame = 3
Query: 261 PTFQQDLESELVVDEGEPLRLMTRV--GPADDGSLEINWFKDGKLLSAGSRFSTIFD--RGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMM--------------QEINNL-----DKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTES----SISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKE------DEIITDLPSFIKQLEPIDQLNEGDSV 1550
P F + L++ + V EG + L + P D I W K+ ++S + +G I T D+ Y A + +G + R+N E V+Y +PE + EI L + +E G + E+ + P F +L S+ + + P+H E L D ++V EW DG L+ +R+ +I + GYC+LD Y D+G +C A N G A+ S + E+S++ S L E + +++ + + G D P + + P I V EG A ++ + K+ +WY NG +++ R + I L I D+G + + G E V L +Q Q + I EL AP+A E D + D P+ Q + + +L + + V
Sbjct: 979 PQFIEKLKN-ISVKEGSLVELAVKAIGNPLPD----IVWLKNSDIISPHKYPHIKIEGAKGQAHFQIPQTSGSDSAWYTATAINKAG---RDTTRCRVNVE--VEYTEPVPERRLIIPKGTYKAKDVAPPEIEPLHLRYGQEQWEEGDLYDKEKQQK-----PHFKKKL-TSVRMKRFGPVHFECRLTPIGDPTMVVEWLHDGKPLEAANRLRMINEFGYCSLDYEVAYSRDSGVITCRATNKFG-ADQTSATLIVKDEKSLVEDSQLPEGRKVQRMDEMERIAHEGGPSGVTGDDMSEKSKPEIV--LLPEPIRVFEGETAKYRCRVTGYPTPKV--NWYLNGLLIRKSKRFRLHYD-GIHYLEITDCKSYDSGDVRVLAENPEGTAE-HTVKLEIQ---------QKEDFRTILRRAPELKAPEAPGPEPGRVSFDVVKVDKPTDASQAKEVVRLRKAERV 1409 HSP 3 Score: 63.929 bits (154), Expect = 9.764e-10 Identity = 77/321 (23.99%), Postives = 126/321 (39.25%), Query Frame = 3
Query: 1080 MPPSF-------IEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEW 2021
+PPSF IE I+ + + + EG A Q L+ +WY +G + T + +++ L I + SD+G+Y+C +E G E V++++ +K P PSF L+P+ +NEG+++TL+ V L ++W K+ + + + +I++ GTATL + DSGDY ATN+ G S F K+ + D G + F N F P ++ E G A F+ KV G P P ++W
Sbjct: 5119 IPPSFTKKPSTTIEDIEGKVVKI-EGRVA---------GSQPLTVNWYKDGREIFTSDFYDVTFKSSLAVLCIKKSQLSDSGTYVCKATNEAGTASFE-VSVHITAQK----------------------VP-------------PSFDLPLKPV-TVNEGETLTLSCHVR--GSPPLKIQWMKDRRELSSSANTKITFVDGTATLEMTRVSKTDSGDYLCKATNEAG---------SEFCKSKVT-----IKDKPGAPAPAQAAPSPPQ-EVKKFDNL---------FFIEVPKSVHIVEKGTATFIAKVGGDPIPNVKW 5366 HSP 4 Score: 63.1586 bits (152), Expect = 2.001e-9 Identity = 68/283 (24.03%), Postives = 123/283 (43.46%), Query Frame = 3
Query: 258 SPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSA----GSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESII----TKSNLTESSISQLKSLENPGEEYARYNDTSVIPM 1082
+PTF Q L+S +V EG +V + + E++WF+DG++L+A G++ S F G VL I +G + A + +G A S A + + E+ P F+ +L SLT+ QG + +++ + + V +++++G ++ + ++ D +L IA +PED+G YS A NS G+A T + + EE + +K+ ++ S ISQ + AR+ T+ + M
Sbjct: 5 APTFTQPLQS-VVALEGSAATFEAQV--SGNPVPEVSWFRDGQVLTAAALPGAQIS--FSDGRAVLMIPAVTAAHSGRFSVRATNGAGQATST-AELLVTAETAP---------------------------------------PNFIQRL-QSLTVRQGSQVRLDVRVT--GIPAPVVKFYREGAEIQSSADFQIVQDGDLYSLLIAEAFPEDSGTYSVSASNSSGRA-TSTAELLVQGEEEAVPAKKSKTIISASQISQTRQTRVEKRVEARFEATATMQM 238 HSP 5 Score: 60.8474 bits (146), Expect = 1.029e-8 Identity = 57/224 (25.45%), Postives = 101/224 (45.09%), Query Frame = 3
Query: 1359 TLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEE--DLASGNISTFQNTEDVQEECLPNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEWR 2024
T VQT + ++ + +G A E A + +KE + P+ I L+ + + EG+SVTL ++ + + + W++ + I N QI+Y++ A L I A +EDSG + AT++ G I+ SC+ K+ + ++ + + E D IS+ + D P F KP+ EGG F+ ++ G P P + W+
Sbjct: 350 TTQVQTSTQVQMEQRWEGSVAGVKEVPETAAIPSAVKEPVV---PPTLIAGLKNV-TVTEGESVTLECQI--SGHPTPAIMWFREDYRIENSIDFQITYEKSYARLMIREAFAEDSGRFTCTATSEAGT-----ISTSCYLLVKVSEEIESREEITVQEKQRTVVEAKDETMSEISSV--SVDTGAPAAPFFVKKPVVQKLVEGGSVVFVCQIGGSPKPHIYWK 560 HSP 6 Score: 55.0694 bits (131), Expect = 5.953e-7 Identity = 55/254 (21.65%), Postives = 107/254 (42.13%), Query Frame = 3
Query: 1062 DTSVIP-------MPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTL-------------NVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQ-LNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISY--DRGTATLNIVSAISEDSGDYWLIATNKNGQTQS 1754
+T+ IP +PP+ I + + ++V+EG + I + W+ ++ E + + L I D+G + C SE G + + L VQ ++ +++ + + +S I ++ + AP A P F+K+ P+ Q L EG SV ++ + +Y W K+ + G R ++S+ D G L I ++D+G+Y + A N++G+T +
Sbjct: 377 ETAAIPSAVKEPVVPPTLIAGL--KNVTVTEGESVTLECQISGHPTPAIM--WFREDYRIENSIDFQITYEKSYARLMIREAFAEDSGRFTCTATSEAGTISTSCYLLVKVSEEIESREEITVQEKQRTVVEAKDETMSEISSVSVDTGAPAA-----------PFFVKK--PVVQKLVEGGSVVFVCQIGGSPKPHIY--WKKSGVPLTTGYRYRVSHNKDTGECKLEISMTFADDAGEYTIFAKNQHGETSA 611 HSP 7 Score: 52.373 bits (124), Expect = 3.136e-6 Identity = 55/250 (22.00%), Postives = 107/250 (42.80%), Query Frame = 3
Query: 261 PTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGT-AQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLV-TEWFKDGMSLKLGSRIDVIY--DRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLT 998
PT L++ + V EGE + L ++ + + I WF++ + F +++ + L I +D+G + C A S +GT + S V+++ E + E+ + D+ + ++ AA F + P L++G + +F+ S W K G+ L G R V + D G C L+I+ + +D G+Y+ A+N G+ + + E+ + K ++T
Sbjct: 392 PTLIAGLKN-VTVTEGESVTLECQI--SGHPTPAIMWFREDYRIENSIDFQITYEKSYARLMIREAFAEDSGRFTCTATSEAGTISTSCYLLVKVSEEIESREEITVQEKQRTVVEAKDETMSEISSVSVDTGAPAA----PFFVKKPVVQKLVEGGSV---VFVCQIGGSPKPHIYWKKSGVPLTTGYRYRVSHNKDTGECKLEISMTFADDAGEYTIFAKNQHGETSASTSLLEEEEFEAYMKKQDVT 631
BLAST of Titin vs. Ensembl Zebrafish
Match: ttn.2 (titin, tandem duplicate 2 [Source:ZFIN;Acc:ZDB-GENE-030113-2]) HSP 1 Score: 117.087 bits (292), Expect = 6.236e-26 Identity = 155/673 (23.03%), Postives = 267/673 (39.67%), Query Frame = 3
Query: 360 EINWFKDGKLLSAGSRFST-IFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPE-EMMQEINNLD------KNYETGTYHGMEEDKHAAKTI-------------PKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSL--KLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSN-DWYHNGEIVKTCSRLTGKLE--MNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTE-------KSIIL--------DPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLTVIPMVDFRPDLRHI----TPENP 2243
+I W+KDGK + G R+ + G L + + +D G Y A + G A ++ ++ ES P+ E ++ + + +Y G + A+ + P F+ Q P+S +GQ ++ + T WF G + +I V+ + G ++ + P+D+G+++ VA+N GK T S +T +E+++ P FIE + + ISV EG+ V G L + W N +I+ K+E + I T SD+ Y ++ GR ++ +NV+ E + +I+ D I +H + + ++ + E P F K+L + ++ V ++ P D ++ VEW + + +R+++ + G +L+ A S DSG ATNK G Q+ I + EK + S L + QR+ ++E G S +D+ E+ P + P I EG AK+ +V+G+PTP + W ++ +L+ + + DG I+YLEI C V V A NP G A+ L + DFR LR PE P
Sbjct: 712 KIAWYKDGKRIKHGGRYQMEVLQDGRASLRLPVVLPEDEGIYTAFASNMKGNA---VSSGKLYVESTAAAQPYYPQAEAVRRVQSTSPMSARSASYSPGRSPARSVSRSPARRLDDTDESQLERLYKPVFV-QKPSSFRCSEGQTARFDLKVVGRPMPD--TFWFHSGQQVVNDYTHKI-VVKEDGTQSMIVVPAMPQDSGEWTVVAQNRAGKT-TVSMTLTVEAKENLVR----------------------------------PQFIEKL--KNISVKEGSLVELAVK---AIGNPLPDIVWLKNSDIISPHKYPHIKIEGAKGQAHFQIPQTSGSDSAWYTATAINKAGR-DTTRCRVNVEVEYTEPVPERRLIIPKGTYKAKDVAPPEIEPLHLRYGQEQWEEGDLYDKEK-QQKPHFKKKLTSV-RMKRFGPVHFECRLTPIGDPTMVVEWLHDGKPLEAANRLRMINEFGYCSLDYEVAYSRDSGVITCRATNKFGADQTSATLI--VKDEKSLVEDSQLPEGRKVQRMDEMERIAHEGGPSGVTG-DDMSEKSKPEIVLLPEPIRVFEGETAKYRCRVTGYPTPKVNWYLNGLLIRKSKRFRLHYDG-IHYLEITDCKSYDSGDVRVLAENPEGTAEHTVKLEIQQKEDFRTILRRAPELKAPEAP 1330 HSP 2 Score: 82.4185 bits (202), Expect = 2.416e-15 Identity = 145/639 (22.69%), Postives = 235/639 (36.78%), Query Frame = 3
Query: 249 LYDSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFK-DGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLVVPDSVCK-TYNDGSINYLEINRCSELGDIKVHVAATNPLG 2159
+ + P F + LE + V G+ + L ++G + ++I+WFK DGK+ S+ + F +G L + + D G+Y C A++ G+A S+ +LT +Q++ KT P F ++ S+ I+GQP+ E + S + W KDG +L+ S + +D L+IA +G+Y+CVA NS+G A SC ++ LT L+ P RY PP F + + P ++SV + +T + W + + ++T N L IL +D+G Y C ++ G+ D S +S +KE +I PSF K+ + EG V + +V A L V WYK+ I +++ A L I + DSG Y ATN+ G + FE +TA ++ P+FD+ +T EG V G P ++W D + + K T+ DG+ LE+ R S+ ATN G
Sbjct: 6631 ILEPPFFIESLEP-MEVTAGDAVCLKCQIGGTPE--IKISWFKADGKVRSSPTCKMEFF-KGIACLKLAKVAKSDIGEYTCKAENSIGSATSSC------------HLT------VQDV----------------------KTPPSFPKKI-TSIMQIEGQPVQFECRVA--GSSPMEVSWLKDGEALRSDSEYTMSFDDNSAVLNIAKGEMRHSGEYTCVATNSVGSA---SC------------RAKLT---------LQEP-----RY--------PPVFDKKLVPVDVSVGDTVELECHMT----GSMPIKVTWSKDHKDIRTGGNYKISCVENTPHLTILKADKADSGRYSCHASNDVGK------------------DSCSTEVS---------------VKERKI---PPSFTKKPSTTIEDIEGKVVKIEGRV--AGSQPLTVNWYKDGREIFTSDFYDVTFKSSLAVLCIKKSQLSDSGTYVCKATNEAG--------TASFEVSVHITA-----------------------------------QKVPPSFDLPLKPVTVNEGETLTLSCHVRGSPPLKIQWMKDRRELSSSANTKITFVDGTAT-LEMTRVSKTDSGDYLCKATNEAG 7099 HSP 3 Score: 75.8702 bits (185), Expect = 2.349e-13 Identity = 109/463 (23.54%), Postives = 181/463 (39.09%), Query Frame = 3
Query: 261 PTFQQDLESELVVDEGEPLRLMTRV--GPADDGSLEINWFKDGKLLSAGSRFSTIFD--RGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMM--------------QEINNLDKNY-----ETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTE----SSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKE------DEIITDLPSFIKQLEPIDQLNEGDSV 1550
P F + L++ + V EG + L + P D I W K+ ++S + +G I T D+ Y A + +G + R+N E V+Y +PE + EI L Y E G + E+ + P F +L S+ + + P+H E L D ++V EW DG L+ +R+ +I + GYC+LD Y D+G +C A N G A+ S + E+S++ S L E + +++ + + G D P + + P I V EG A ++ + K+ +WY NG +++ R + I L I D+G + + G E V L +Q Q + I EL AP+A E D + D P+ Q + + +L + + V
Sbjct: 935 PQFIEKLKN-ISVKEGSLVELAVKAIGNPLPD----IVWLKNSDIISPHKYPHIKIEGAKGQAHFQIPQTSGSDSAWYTATAINKAG---RDTTRCRVNVE--VEYTEPVPERRLIIPKGTYKAKDVAPPEIEPLHLRYGQEQWEEGDLYDKEKQQK-----PHFKKKL-TSVRMKRFGPVHFECRLTPIGDPTMVVEWLHDGKPLEAANRLRMINEFGYCSLDYEVAYSRDSGVITCRATNKFG-ADQTSATLIVKDEKSLVEDSQLPEGRKVQRMDEMERIAHEGGPSGVTGDDMSEKSKPEIV--LLPEPIRVFEGETAKYRCRVTGYPTPKV--NWYLNGLLIRKSKRFRLHYD-GIHYLEITDCKSYDSGDVRVLAENPEGTAE-HTVKLEIQ---------QKEDFRTILRRAPELKAPEAPGPEPGRVSFDVVKVDKPTDASQAKEVVRLRKAERV 1365 HSP 4 Score: 74.7146 bits (182), Expect = 4.659e-13 Identity = 98/482 (20.33%), Postives = 157/482 (32.57%), Query Frame = 3
Query: 297 VDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNG 1742
V G+ L +V + D L++ WFKDGK ++ G ++ F VL IL D+ +Y + G Q + + IN LDK IP +++ + G + +E + + WFK+G + G++ L I D G ++C A N+ G +ET + T + PP F + P+ V G W+ + + T K E N S+L + PS + Y C + ++ G+V S AL + + P F+ +L+ + G TL KV A + + WYKNE I + + Q+++ ATL I S EDSGDY A++ G
Sbjct: 4665 VTAGDSATLECKVSGSPD--LKVKWFKDGKEMAGGRKYKITFKENVAVLKILSAERGDSSEYKVEVSNRVGKEQCSCS-----------------------INVLDK------------------IIPPTFTKMLKKVDGNVGASIQMECKVS--GSQPITISWFKEGKDITTGTKYQTEMQESTALLKITNLETSDAGVFTCHATNAAGHSET----------------------------------------SGTISVKEPPVF--SLKPQSQDVIPGTTVVLTAAFT--GTAPFMIKWFREDKEMLTGGTCFIKKETNSSSLELHALKPSQSAKYTCQVSNDAGKV------------------------SCTAAL---------------FVKEPPKFVMKLDLTKLVLNGSLATLECKV--AGSPEISIRWYKNETEIGSDDKYQMAFTDSVATLRISSCCIEDSGDYICQASSDAG 5016 HSP 5 Score: 73.9442 bits (180), Expect = 8.564e-13 Identity = 131/667 (19.64%), Postives = 220/667 (32.98%), Query Frame = 3
Query: 357 LEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTL---------------NVQTEKSIILDPQSKGISAIHA-------------------------LEAELNAPK-------AEMKEDE---------IITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLTV 2189
L+ WFKDG LS+G ++ F + L +L T D G+Y K+ G+ + ++ LDK +P S+ I G+ + E+ + + L WF + + + G ++ + C L + D+G Y C A NS G AET + + V+ PPSF+ P+ + G F T+ KL W+ + + + L + + L + S G Y C + ++ G+ E+ PV L +V+ KS+IL+ G I LE LN+ K E+ D I + P FI+ LEP++ + GD+V L K + + + W+K + + + ++ + +G A L + D G+Y A N G A S+ + ++D++ P+F K +I EG P +F +V+G + W D + DS D + L I + + ATN +G A A LT+
Sbjct: 6289 LKPKWFKDGVELSSGKKYKITFSKMISSLKLLSTERSDTGEYTFSIKNEVGS-----------------------DSCAMQLTVLDK------------------IVPPLFSRKLKDTQSIVGKSM--ELDCKASGSAPLTISWFHNEVEIMSGPNYEITFTENTCTLKVPTLKLSDSGTYKCKAVNSAGAAETSA----------------------------------------SLVVKEPPSFV--TTPQPVEALPGTTVTFTATVQGSTPMKLK--WFRGSKEIVSGRSCEIALRGDTAILELHNIDKSHAGEYTCQIINDAGK-ENCPVNLFVKEAAHFVKKLKNLSVEMGKSLILECTYAGSPKILVKWHKDGQEIYSSYKYNITTTESSCILEC-LNSDKEAAGKYTCEVSNDAGHDICEAAVSILEPPFFIESLEPME-VTAGDAVCL--KCQIGGTPEIKISWFKADGKVRSSPTCKMEFFKGIACLKLAKVAKSDIGEYTCKAENSIGS-----------------------ATSSCHLTVQDVKTP--------------------PSFPKKITSIMQIEGQPVQFECRVAGSSPMEVSWLKDGEALRSDSEYTMSFDDNSAVLNIAKGEMRHSGEYTCVATNSVGSASCRAKLTL 6820 HSP 6 Score: 73.1738 bits (178), Expect = 1.496e-12 Identity = 137/650 (21.08%), Postives = 220/650 (33.85%), Query Frame = 3
Query: 351 GSLEIN--WFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQS-----------------------------CNVTCHPE-----------------------ESI----ITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMN--ISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLE-PIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLVVPDSVCKTYNDGSINYLEINRCSEL 2117
GSL IN WFK+ K + A + IF L+I D+G+Y C+AK+ +G+ Q +GT E P + + P S +I G + + L L WFKD + G V + ++++ F P D+G Y C N +G Q+ C +T PE +S+ I S++ +S + ++ G E + + PP ++P+ P E V G+ AYF+ + W + + +K+ + L+ N I L++ D G Y CV+ +E G S+ TL+++ + PSF++++E + L + V+ P L ++W K+E+ I+ +Q +++ ATL I S SG Y A N+ GQ + CF S+L+ VQE P KP I G P F KVSG P ++W D V+P S++Y IN S+L
Sbjct: 5270 GSLPINVTWFKENKEIEADEKHKCIFFENAASLEISRLDSSDSGNYTCIAKNQAGSDQC-----------------------------------SGTLKVKE---------PPSIIEKPESQDVIPGSKVQFNVLLS--GTPPLTINWFKDKKEVSSGIDCSVQKNDTSSSIELFFAKPSDSGQYVCEVSNDVGSDSCQATLFVKEPPKFTRKPEKITVVKPSQLVVFECQITGTPEIDTYWFKDGNDISPSDKYKMTFVDSLARLEIISSDIKDSGVYYCEARNEAGSESCSMDLR--VKEPPVLVKPLSPLE--VVNGSNAYFECLVK--GTAPFEVTWQKDSKDIKSSLKHV-ILQKNDSIITLDVQKCDALDVGEYQCVVANEVGSCSSQS-TLSIK--------------------------------------EPPSFVERIENVVTVLGKMVEFKCVVRGSPP----LSIQWQKDESWILEDPSIQRTFENNVATLTIPVCESIHSGKYTCQAMNEAGQEK-------CF--------STLV-----------------------------VQEP--PQIIEKPEVINVTAGDPVSFECKVSGTPELKVKWSKDGKEVIPS------RQHSLSY--INNVSQL 5769 HSP 7 Score: 69.3218 bits (168), Expect = 2.237e-11 Identity = 120/514 (23.35%), Postives = 192/514 (37.35%), Query Frame = 3
Query: 291 LVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPE-------------------ESIITKSNLTESSIS-------QLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKV--EPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISE-DSGDYWLIATNKNGQ 1745
L V+ G+P R V +++W+K+ + LS+G + + + L ++ +D Y C AK+ G A S+ + +N E +PE + D A + P L+ + N+ + +GQ + + S L W+ +K V C L+I YPED G+Y+CVARNS G SC+ + S+ K+ E + S + K+LE E+ + P F + P E+++ G F VT+ K+ W+HNG+ + + S T E + +L I G Y C + G QT IL + +SA A K K +I P F Q++P+ Q EG V KV P D V+W+K + I + + + + I+S I + DSG Y A N G+
Sbjct: 2629 LSVEAGKPARFSVEVTGIPQP--QVSWYKNSQALSSGFKCKFLREGNEHTLLLIEVFPEDAAQYNCEAKNDYGVATSSAS---LNVE--------VPE-------------------VVSPDTGAPLSPPVVLTPIQNT-SANEGQSARFQCRV---SGEDLTITWYCKDKEIKQSDIFRVSQFDENCQLEITRVYPEDEGEYTCVARNSAGMV---SCSALLKVDVTRVREEIEAKIEEEVKEFSSLTVKTEEEEETYSTSLHLPEKSKTLELKPEQKRFSSSLERKREKPVFESKLTPAEVTI--GESVRFTVTVSGFPKPKV--QWFHNGKAITSSSIYTFVEERDEYSLIITKVKKDYEGEYSCTASNRFG-----------QTTCKTILKVELSQLSA---------AEKWVEKMFKIPGQPPCFTTQIQPV-QCVEGSEVKFLYKVTGTPFPD----VQWFKGNSQIKSSQTCSVVCNPDGSGFLIMSNIQQRDSGLYTCKAVNPFGE 3074 HSP 8 Score: 67.0106 bits (162), Expect = 1.276e-10 Identity = 151/694 (21.76%), Postives = 240/694 (34.58%), Query Frame = 3
Query: 357 LEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEED--------------------------KHAAKTIPKFLSQLPNSLTLIQGQ-----PLHIEI-----FLEHHSDSSL-----------VT-------EWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKA-ETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWY-HNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVE-SEPVTLNVQTEKSIILDPQSKGISAIHALEAE-----------LNAPKA----EMKED--------EIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLVVPDSVCKTYN--DGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLTVI 2192
+++ W KDG+ L R+ +R L I D G+Y VA S A + G D P E +EI L+K T + E+D K TI + T I G+ LH+ I + H S+ VT W+K+ +L G + + + L + +PED Y+C A+N G A + S NV ++ + +P DT PP + PI + S +EG A F+ + +G+ L+ WY + EI ++ + + N L I P D G Y CV + G V S + ++V + I + + +L + L+ P+ E+K + E + P F +L P + + G+SV TV V + V+W+ N I + S +R +L I + G+Y A+N+ GQT + I V S L A +++ + P F + + EG KFL KV+G P P ++W + + C DGS +L ++ + A NP G A A L V
Sbjct: 2466 VQVTWMKDGQELELSERYKVSTERHVHRLMIQTVRMSDAGEYSVVAGSSVSKANLTV--------EGKDVRISEPAE--KEITVLEKQRSTFEFEVNEDDIEGRWLRNGVEIQFSVDQRFNYAIIRKIHRLTITETYRSDAGEYTFIAGKNRSIVTLHVNIPEPPQIIRHMQPLSVEAGKPARFSVEVTGIPQPQVSWYKNSQALSSGFKCKFLREGNEHTLLLIEVFPEDAAQYNCEAKNDYGVATSSASLNV--------------------EVPEVVSP--------DTGAPLSPPVVLTPI--QNTSANEGQSARFQCRV---SGEDLTITWYCKDKEIKQSDIFRVSQFDENCQ-LEITRVYPEDEGEYTCVARNSAGMVSCSALLKVDVTRVREEIEAKIEEEVKEFSSLTVKTEEEEETYSTSLHLPEKSKTLELKPEQKRFSSSLERKREKPVFESKLTPAE-VTIGESVRFTVTV--SGFPKPKVQWFHNGKAITSSSIYTFVEERDEYSLIITKVKKDYEGEYSCTASNRFGQTTCKTI--------LKVELSQLSAAEKWVEKMFKIPGQP-------------------PCFTTQIQPVQCVEGSEVKFLYKVTGTPFPDVQWFKGNSQIKSSQTCSVVCNPDGS-GFLIMSNIQQRDSGLYTCKAVNPFGEASCSAELIVF 3084 HSP 9 Score: 65.4698 bits (158), Expect = 3.342e-10 Identity = 96/493 (19.47%), Postives = 170/493 (34.48%), Query Frame = 3
Query: 267 FQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGE-IVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNG 1742
F Q L + VV GEPL+L +V L I+W+K+ +LS G FD VL+I + DDNG Y C A++ +GT + A P P F +LP + ++G+ + L+ + + WFKD LK + + + L I D G+Y C A N++G S+L + S+ + PP+F++ + + G T+ Q ++ W + + I++ E N L + + G Y C L ++ G E L V +I+ S ++A A E +V+ T +++P +W+K+ + +G + +I++ + ++L ++S D+G+Y N+ G
Sbjct: 5981 FVQKLPAAKVVKMGEPLQLECKV--TGTAPLRISWYKNDAILSDGGNLRMTFDNSVAVLEISTSSFDDNGVYTCEAQNDAGTKSCSTALTIKEP-------------------------------------------PSFY-KLPTPVEGLKGKDASLNCELKGSAPFEIT--WFKDKKQLKESRKYKFVSEGCSATLHILGLEASDAGEYECKATNNVG--------------------SDLCQGSVK--------------------LREPPAFVKKLS--NTTAISGEEVVLLTTVK--GSQPMTVSWVQDKDHILRDGENRKITFENNEVTLKVFKADSTTAGKYTCQLKNDAGVAEC-TANLTVLEPAAIVDKTDSISVTAGEAAALEC---------------------------------TVSGTPELKP--------KWFKDGVELSSGKKYKITFSKMISSLKLLSTERSDTGEYTFSIKNEVG 6339 HSP 10 Score: 62.3882 bits (150), Expect = 3.161e-9 Identity = 68/283 (24.03%), Postives = 123/283 (43.46%), Query Frame = 3
Query: 258 SPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSA----GSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESII----TKSNLTESSISQLKSLENPGEEYARYNDTSVIPM 1082
+PTF Q L+S +V EG +V + + E++WF+DG++L+A G++ S F G VL I +G + A + +G A S A + + E+ P F+ +L SLT+ QG + +++ + + V +++++G ++ + ++ D +L IA +PED+G YS A NS G+A T + + EE + +K+ ++ S ISQ + AR+ T+ + M
Sbjct: 5 APTFTQPLQS-VVALEGSAATFEAQV--SGNPVPEVSWFRDGQVLTAAALPGAQIS--FSDGRAVLMIPAVTAAHSGRFSVRATNGAGQATST-AELLVTAETAP---------------------------------------PNFIQRL-QSLTVRQGSQVRLDVRVT--GIPAPVVKFYREGAEIQSSADFQIVQDGDLYSLLIAEAFPEDSGTYSVSASNSSGRA-TSTAELLVQGEEEAVPAKKSKTIISASQISQTRQTRVEKRVEARFEATATMQM 238 HSP 11 Score: 55.4546 bits (132), Expect = 4.233e-7 Identity = 44/165 (26.67%), Postives = 73/165 (44.24%), Query Frame = 3
Query: 1530 LNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEWR 2024
+ EG+SVTL ++ + + + W++ + I N QI+Y++ A L I A +EDSG + AT++ G I+ SC+ LL + +D IS+ + D P F KP+ EGG F+ ++ G P P + W+
Sbjct: 381 VTEGESVTLECQI--SGHPTPAIMWFREDYRIENSIDFQITYEKSYARLMIREAFAEDSGRFTCTATSEAGT-----ISTSCY----------LLVKA----------KDETMSEISSV--SVDTGAPAAPFFVKKPVVQKLVEGGSVVFVCQIGGSPKPHIYWK 516 HSP 12 Score: 55.0694 bits (131), Expect = 5.833e-7 Identity = 51/216 (23.61%), Postives = 94/216 (43.52%), Query Frame = 3
Query: 291 LVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLV-TEWFKDGMSLKLGSRIDVIY--DRGYCALDIAFCYPEDNGDYSCVARNSLGK 929
+ V EGE + L ++ + + I WF++ + F +++ + L I +D+G + C A S +GT ++ L + +E M EI+++ + +TG A P F+ + P L++G + +F+ S W K G+ L G R V + D G C L+I+ + +D G+Y+ A+N G+
Sbjct: 379 VTVTEGESVTLECQI--SGHPTPAIMWFREDYRIENSIDFQITYEKSYARLMIREAFAEDSGRFTCTATSEAGTISTSCY-----------LLVKAKDETMSEISSV--SVDTG-----------APAAPFFVKK-PVVQKLVEGGSV---VFVCQIGGSPKPHIYWKKSGVPLTTGYRYRVSHNKDTGECKLEISMTFADDAGEYTIFAKNQHGE 564 HSP 13 Score: 51.9878 bits (123), Expect = 4.203e-6 Identity = 42/171 (24.56%), Postives = 77/171 (45.03%), Query Frame = 3
Query: 1251 EMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQ-LNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISY--DRGTATLNIVSAISEDSGDYWLIATNKNGQTQS 1754
E + + L I D+G + C SE G + T +++ + + +S I ++ + AP A P F+K+ P+ Q L EG SV ++ + +Y W K+ + G R ++S+ D G L I ++D+G+Y + A N++G+T +
Sbjct: 421 EKSYARLMIREAFAEDSGRFTCTATSEAG---------TISTSCYLLVKAKDETMSEISSVSVDTGAPAA-----------PFFVKK--PVVQKLVEGGSVVFVCQIGGSPKPHIY--WKKSGVPLTTGYRYRVSHNKDTGECKLEISMTFADDAGEYTIFAKNQHGETSA 567
BLAST of Titin vs. Ensembl Zebrafish
Match: ttn.1 (titin, tandem duplicate 1 [Source:ZFIN;Acc:ZDB-GENE-030616-413]) HSP 1 Score: 108.612 bits (270), Expect = 2.650e-23 Identity = 154/654 (23.55%), Postives = 262/654 (40.06%), Query Frame = 3
Query: 360 EINWFKDGKLLSAGSRFST-IFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKT---------IPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSL--KLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSN-DWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGS----YICVLFSEHGR------VESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPK--AEMKEDEIITDL-----PSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLVVPDSVCKTYNDGSINYLEI--NRCSELGDIKVHVAATNPLGVAKEEATLTVIPMVDFRPDLRH 2225
+I W+KDGK + G R+ + G L + + +D Y + + G A ++ ++ ES TQL + + T G + +T P F+ Q P+S+ +GQ ++ + T WF +G + +I V+ + G ++ + P D+G+++ VA+N GK T S +T +E+++ P F+E + R ISV EG V G L + W N +I+ ++E N + +PS GS Y ++ GR V E + Q EK +++ + I A E E + E ED + D P F K+L + +L ++ P D ++ VEW + + +R+++ + G +L+ +A S DSG ATNK G Q+ I + EK + + L + QR+ ++E G S +++ E+ P + P + EG AKF +V+G+PTP + W ++ +L+ + DG I+YLEI + + GD+K V+A NP GV + + DFR LR
Sbjct: 769 KIVWYKDGKRIIHGGRYQMEVLRDGRASLRLPTVLAEDEAVYTALISNIKGNA---VSSGKLYVESAGVAQTQLSQPGAVQRIRSTSPRSTSRSPGRSPSRRLDETDEAQLERLYKPVFV-QKPSSVKCSEGQTARFDLKVVGRPMPE--TYWFHNGQQVVNDYTHKI-VVKEDGTQSMIVVPAMPLDSGEWTVVAQNRAGKT-TVSMTLTVEAKENLVR----------------------------------PQFVEKL--RNISVKEGTLVELAVK---AIGNPLPDIVWLKNSDIISPHKHPHIRIEGGKGEAN--FKIPSSAGSDSAWYTATAINKAGRDTTRCRVNVEVDAVKPQPEKKLVIPKGTYKAKEIAAPELEPLHLRYGQEQWEDGDLYDKEKQQKPHFKKKLTSV-RLKRFGPAHFECRLTPIGDPTMIVEWLHDGKPLAAANRLRMVNEFGYCSLDYEAAYSRDSGVITCRATNKFGVDQTSATLI--VKDEKSLVEETQLPEGRRGQRIDEIERIAHEGGPSGVTGDDNL-EKTKPEIVLLPEPVRVMEGETAKFRCRVTGYPTPKVNWYLNGLLIRKSKRFRLQFDG-IHYLEIVDTKSYDSGDVK--VSAENPEGVTEHVVKFEIQQKEDFRSILRR 1366 HSP 2 Score: 73.559 bits (179), Expect = 1.072e-12 Identity = 136/656 (20.73%), Postives = 245/656 (37.35%), Query Frame = 3
Query: 291 LVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDR--GFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQS------------CNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREIS-------------------VSEGAPAYFKVTIDPGNGQKLSN-DWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGR--------------------VESEPVTLNVQTE----KSIILDPQSKGISAIHALEAELNAPK-------AEMKEDEIITDLPSFIKQLEP--IDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQ------EECLPNFDVKPMNIT------AGEGGPAKFLVKVSGFPTPTLEW 2021
L V+ G+P R V +++W+K+ + LS+G F F R L ++ +D Y C AK+ G A S+ + + E++ + + + A + P ++ L ++ ++ +G+ + + S L W++ + + + C LDI+ Y D G+Y+CVARNS G + +V+ H E ++T +TE+ S ++S E+ S + M + ++ I+ ++++ VS G A F + +GQ + W HNG + R+ E + +L IL D GSY C + + +G V S+ ++ + +TE K + + + + AL + ++ D+ P F+ +L P + + E DS+ K P S ++ W N+ ++ + +D LN+ + +E G N G + +C K+ TA S L EED + ++ T ++Q E + KP+ IT A G AKF V VSGFP P ++W
Sbjct: 2676 LSVEAGKPARFSVEV--TGIPQPQVSWYKNSQALSSG--FKCKFQREGNEHTLLLIEVFPEDAAQYNCEAKNDYGVATSSAS-------------------LNVEVSEV-----------VSPETVAPVSAPVIITSLQDT-SVEEGESARFQCRV---SGDDLKITWYRRDKEIVMSDNFQMTQFGDTCQLDISRAYVADAGEYTCVARNSGGMVSCSAVLTVNDHSVSTVASVSTHSE--MVTSQTMTETRSSTMESFES----------VSTVMMSSTSVKSIEVQQVTSEEEYSTSPVLTLKMSDVIVSLGDVAQFNCSF---SGQPFTEIVWDHNGRTITNTERVQYAHEAGLISLIILNVHLGDQGSYCCTVKNRNGESRTSAQLTVEAKEANPKETPVPSDSISKSAKTESESSKKATDQEKVESLQNVGALTTKWQISHHYNPDVTTLLERDKYDAIQPEFLVKLSPEFLAKQREIDSIYYINKGSP----SPFIVWLYNQLNVEEPRSYSLKHDGPLCCLNVKNVNTEQGGSNSCKVINAAG-------DKTCSTHLKMKTAFSTLPIPT--------EEDSYATSLRLKYQTFELQPESQLFSESIKKKPEKPIFITKLSSECAALGETAKFTVIVSGFPKPIIQW 3259 HSP 3 Score: 70.8626 bits (172), Expect = 8.494e-12 Identity = 70/280 (25.00%), Postives = 118/280 (42.14%), Query Frame = 3
Query: 150 VIPNGTTMAPHALEVVNQVNKRSLASP-------RRGSDH-----LYDS-----PTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPE-EMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAE 935
VIP GT A + +A+P R G + LYD P F++ L S + G P R+ P D ++ + W DGK L+A +R + + G+ LD D+G C A + G Q++ A + + E + TQLPE Q I+ +++ G G+ D + KT P+ + LP + +++G+ + + + W+ +G+ ++ R + +D G L+I D+GD A N G E
Sbjct: 1086 VIPKGTYKA------------KEIAAPELEPLHLRYGQEQWEDGDLYDKEKQQKPHFKKKLTSVRLKRFG-PAHFECRLTPIGDPTMIVEWLHDGKPLAAANRLRMVNEFGYCSLDYEAAYSRDSGVITCRATNKFGVDQTS-ATLIVKDEKSLVEETQLPEGRRGQRIDEIERIAHEGGPSGVTGDDNLEKTKPEIV-LLPEPVRVMEGETAKFRCRVTGYPTPKV--NWYLNGLLIRKSKRFRLQFD-GIHYLEIVDTKSYDSGDVKVSAENPEGVTE 1347 HSP 4 Score: 70.8626 bits (172), Expect = 8.566e-12 Identity = 82/324 (25.31%), Postives = 129/324 (39.81%), Query Frame = 3
Query: 786 WFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIIT---KSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQ-LNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQ 1745
W KDG +L G + ++ + G L+I D+G Y+C A+NS+G T +C +T + KS LTE + + EN EE Y + ++++S+G + +I + K W NG + + + L I D G C +E G V+ + D +SA H+ PSF+ ++P Q +NEG +VT T ++ + S VEW K+ I S M++S + TL I +A EDSG + + A NK GQ
Sbjct: 28684 WMKDGKNLSQGGKYEIFEEHGSAHLEIYDADLSDSGVYTCTAQNSVGSVST-NCTLTIQARTTAAALEGKSELTEEIVKK----ENTYEEVQSYTEIKA-----------SKTQMTISQGQTVTLRASIPEASDVK----WILNGAELSNSESYRYGVSGSDHTLTIKSISHHDQGILTCEARTEQGVVKCQ-------------FD---MTVSATHS-------------------GSPSFL--VQPHSQNVNEGQNVTFTCEI--TGEPSPEVEWLKDNAVISITSNMKLSRSKNVYTLEIHNATIEDSGKFTVKAKNKFGQ 28948 HSP 5 Score: 67.3958 bits (163), Expect = 9.115e-11 Identity = 112/500 (22.40%), Postives = 180/500 (36.00%), Query Frame = 3
Query: 360 EINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSG----TAQSNIAHVRINP------------------ESGVDYLTQLPEEMMQEINNLDKNYETGTYHG------MEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNV-----TCHPEESIITKSNLTESSIS-QLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTL--TVKVEPANDNSLYVEWYKNENHIINGSRMQISYDR-GTATLNIVSAISEDSGDYWLIATNKNGQT 1748
EI W +G+ ++ R + G I L IL D G Y C K+ +G +AQ + NP ES Q E +Q + L ++ ++ +E DK+ A P+FL +L L + + + ++ S S + W + ++++ + +D C L++ E G SC N+ G ++C+ T I T+ + +S+ + ++ E E P P FI + ++ E A K T+ K WYHNG ++ + S T E +L I G Y C + G+ S S +H ++ K+ E I P FIK++E I Q G TV P D V WY+ NH+ I + G+ L ++ DSG Y ATN G+T
Sbjct: 2956 EIVWDHNGRTITNTERVQYAHEAGLISLIILNVHLGDQGSYCCTVKNRNGESRTSAQLTVEAKEANPKETPVPSDSISKSAKTESESSKKATDQEKVESLQNVGALTTKWQISHHYNPDVTTLLERDKYDA-IQPEFLVKLSPEF-LAKQREIDSIYYINKGSPSPFIV-WLYNQLNVEEPRSYSLKHDGPLCCLNVKNVNTEQGGSNSCKVINAAGD---KTCSTHLKMKTAFSTLPIPTEEDSYATSLRLKYQTFELQPESQLFSESIKKKPEKPIFITKLSSECAALGETA----KFTVIVSGFPKPIIQWYHNGNLINSSSFYTFVQEKEEHSLVITQITKELEGEYSCTASNRFGK---------------------STCTSYLHVKVKDIK------KQGETIGQPPYFIKEIEHI-QCAVGSQALFEYTVAGSPLPD----VHWYRGSNHMQPSEYCIIVNNADGSGYLKMLDIQQSDSGTYSCKATNPYGET 3413 HSP 6 Score: 65.4698 bits (158), Expect = 3.549e-10 Identity = 59/238 (24.79%), Postives = 103/238 (43.28%), Query Frame = 3
Query: 261 PTFQQDLESELVVDEGEPLRLMTRVG----PADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSN---IAHVRINPESGVDYLTQLPEEM----MQEINNLDKNYETGT-YHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIY--DRGYCALDIAFCYPEDNGDYSCVARNSLGKA 932
PT L++ + V EGE + L ++ PA I WF++ + F +++ + L I +D+G + C A S +GT ++ + V ES D+ + +PE + Q ++ ET + + E A F + P L++G + + + + W K G+ L G R V Y D G C L+I+ + +D G+Y+ ARN LG++
Sbjct: 393 PTIVAALKN-VTVTEGETVALECQISGYPTPA------IMWFREDYRIENSIDFQITYEKSYARLMIREAFAEDSGRFTCTATSEAGTISTSCYLLVKVSEEIESREDF-SNVPEHVEMSAQQRQGAIEAKEETMSEISAVSEPVEAGAGAAPFFVKKPTVQKLVEGDRVVFDCQVGGTPKPHIY--WKKSGIPLTTGYRYRVAYNKDTGACKLEISMTFADDAGEYTVFARNPLGES 620 HSP 7 Score: 64.3142 bits (155), Expect = 8.396e-10 Identity = 113/556 (20.32%), Postives = 183/556 (32.91%), Query Frame = 3
Query: 363 INWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDN-GDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALD-IAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPRE-ISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEW 2021
+ W+KDGK L A R++T L+ L+ + G Y C + G +I H + SG +F+ L + +L QGQ L +E + + W+KDG R + C L+ + C G YSC N G CH +++T TE I PP+F + P E I +EG K+ Q LS WY + + + T + N++ + + +D+G Y C +E G + V +N+ +K P+F L+P+ ++EG+ + L+ V + ++W K+ + + + ++++ GTATL I A D+GDY ATN G + AG N ++E P +I E G A F+ KV G P P ++W
Sbjct: 4915 VTWYKDGKQLYASYRYNTTVVGNTCTLECLHECTKETPGKYSCEVSNSYG---KDICHAEVTTFSGP---------------------------------------ARFVKTLTDQ-SLQQGQKLRLEASFK--GIGKIFVTWYKDGKQAYASYRCNTKVIGNTCVLEYLHECDENTPGKYSCEVMNEDGSD-------ICH---ALVTLG--TERKI------------------------PPTFTK--KPSEHIEDAEGK--LVKLESRVSGSQPLSVIWYKDNKEINTSDIYDVSFKSNVAVMCLRKATVTDSGVYTCSASNEAGTASFQ-VVVNITEQKR-----------------------------------PPTFDIPLKPM-TVSEGERLNLSCHV--CGSPPVKIQWMKDRREVTSSASTKMTFVDGTATLEINQASKTDAGDYLCKATNNAG-------------------------NKAGVAPAAAPAAPTTPQATKKLDNLFFIEE---------PKSIHIVEKGTATFIAKVGGDPIPNVKW 5312 HSP 8 Score: 60.4622 bits (145), Expect = 1.074e-8 Identity = 115/536 (21.46%), Postives = 193/536 (36.01%), Query Frame = 3
Query: 672 TIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPE---------------ESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMP--------PSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSF--IKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLTVIPMVD 2204
T+P + P SLT+ +G+ + ++ S+ W ++ + R + +I D G+Y+ V NS GK E Q P ES KS S ++KS E P + R ++ P PSF + ++IS S A K+T+ K S W +G+ + + E + L I SD+G Y C + G V + TL +Q + ALE + + +K++ ++ S+ IK + +++G +VTL + A+D V+W N + N + TL I S D G I T + ++E+ + C F T P+F V+P + EG F +++G P+P +EW D+ ++ S K ++ LEI+ + K V A N G A+L V+ +V+
Sbjct: 28493 TLPATILTKPQSLTVSEGESVKFTCDVDGEPAPSVT--WMREAQIIVSSQRHLITSTEYKSTFEINKVEMSDGGNYTVVVENSEGKQEAQFSLTIRKPTPVQKVVASPPRVKSPESPRIKSPFGIRSPQRIKSPE-PIKSPTRVKSPPMVKSPTPKDKTSKPSFSTEL--KDISAS--ADTIVKLTVKLSGDLKPSISWMKDGKNLSQGGKYEIFEEHGSAHLEIYDADLSDSGVYTCTAQNSVGSVSTN-CTLTIQAR------------TTAAALEGKSELTEEIVKKENTYEEVQSYTEIKASKTQMTISQGQTVTLRASIPEASD----VKWILNGAELSNSESYRYGVSGSDHTLTIKSISHHDQG----ILT---CEARTEQGVVKC-----------------------------------QFDMTVSATHSGSPSFLVQPHSQNVNEGQNVTFTCEITGEPSPEVEWLKDNAVISITSNMKLSRSKNVYTLEIHNATIEDSGKFTVKAKNKFGQCSATASLNVLTLVE 28962 HSP 9 Score: 59.6918 bits (143), Expect = 2.257e-8 Identity = 99/461 (21.48%), Postives = 163/461 (35.36%), Query Frame = 3
Query: 363 INWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLK-LGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNG 1742
+ W KDG+ L RF D+ L I D G+Y VA + A + G D P E ++I ++K T + E++ I G+ W K+G+ ++ R + + R L I Y D G+Y+ +A GK + V+ H + IP P I + P +SV G PA F V + ++S WY N + + + + + E N L ++ P D Y C +++G V + +LNV E S ++ P++ A ++AP IIT L + EG+S +V + + L + WY+ + I+ Q++ T L+I A D+G+Y +A N G
Sbjct: 2516 VMWMKDGQELELSERFRATTDKFLQRLMIQTVRMSDAGEYSVVAGASVSKANLVV--------EGKDVHISEPAE--KDITVVEKQRATFEFEVNEDE--------------------IDGR-------------------WQKNGVEIQSFDQRFNYVTVRKTHRLTITETYRSDAGEYTFIA----GKNRS---TVSLHVQ-----------------------------------IPEAPQIIRHMQP--LSVEAGKPARFSVEVTGIPQPQVS--WYKNSQALSSGFKCKFQREGNEHTLLLIEVFPEDAAQYNCEAKNDYG-VATSSASLNV--EVSEVVSPETV---------APVSAPV-------IITSLQD--------TSVEEGESARFQCRV---SGDDLKITWYRRDKEIVMSDNFQMTQFGDTCQLDISRAYVADAGEYTCVARNSGG 2851 HSP 10 Score: 57.3806 bits (137), Expect = 9.081e-8 Identity = 66/283 (23.32%), Postives = 119/283 (42.05%), Query Frame = 3
Query: 258 SPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSA----GSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESII----TKSNLTESSISQLKSLENPGEEYARYNDTSVIPM 1082
+PTF Q L+S +V EG +V + + E++WF+DG++L+A G++ S F G VL I +G + A + +G A S E++ A+T P Q S+T+ QG + +++ + + V +++++G ++ + ++ D +L IA +PED+G YS A NS G+ T + + EE + TK+ ++ S ISQ + A + T+ + M
Sbjct: 5 APTFTQPLQS-VVALEGSAATFEAQV--SGNPVPEVSWFRDGQVLTAAALPGAQIS--FSDGRAVLMIPAVTAAHSGRFSVRATNGAGQATSTA-------------------ELLA----------------------TAETAPPNFIQRLQSVTVTQGSQVRLDVRVT--GIPTPVVKFYREGAEIQSSADFQIVQDGDLYSLLIAEAFPEDSGTYSVSASNSSGRT-TSNAELLVQGEEVAVPAKKTKTFVSTSEISQTRQARVEKRVEASFESTTSMEM 238
BLAST of Titin vs. Ensembl Zebrafish
Match: ttn.1 (titin, tandem duplicate 1 [Source:ZFIN;Acc:ZDB-GENE-030616-413]) HSP 1 Score: 73.559 bits (179), Expect = 1.196e-12 Identity = 136/656 (20.73%), Postives = 245/656 (37.35%), Query Frame = 3
Query: 291 LVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDR--GFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQS------------CNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREIS-------------------VSEGAPAYFKVTIDPGNGQKLSN-DWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGR--------------------VESEPVTLNVQTE----KSIILDPQSKGISAIHALEAELNAPK-------AEMKEDEIITDLPSFIKQLEP--IDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQ------EECLPNFDVKPMNIT------AGEGGPAKFLVKVSGFPTPTLEW 2021
L V+ G+P R V +++W+K+ + LS+G F F R L ++ +D Y C AK+ G A S+ + + E++ + + + A + P ++ L ++ ++ +G+ + + S L W++ + + + C LDI+ Y D G+Y+CVARNS G + +V+ H E ++T +TE+ S ++S E+ S + M + ++ I+ ++++ VS G A F + +GQ + W HNG + R+ E + +L IL D GSY C + + +G V S+ ++ + +TE K + + + + AL + ++ D+ P F+ +L P + + E DS+ K P S ++ W N+ ++ + +D LN+ + +E G N G + +C K+ TA S L EED + ++ T ++Q E + KP+ IT A G AKF V VSGFP P ++W
Sbjct: 983 LSVEAGKPARFSVEV--TGIPQPQVSWYKNSQALSSG--FKCKFQREGNEHTLLLIEVFPEDAAQYNCEAKNDYGVATSSAS-------------------LNVEVSEV-----------VSPETVAPVSAPVIITSLQDT-SVEEGESARFQCRV---SGDDLKITWYRRDKEIVMSDNFQMTQFGDTCQLDISRAYVADAGEYTCVARNSGGMVSCSAVLTVNDHSVSTVASVSTHSE--MVTSQTMTETRSSTMESFES----------VSTVMMSSTSVKSIEVQQVTSEEEYSTSPVLTLKMSDVIVSLGDVAQFNCSF---SGQPFTEIVWDHNGRTITNTERVQYAHEAGLISLIILNVHLGDQGSYCCTVKNRNGESRTSAQLTVEAKEANPKETPVPSDSISKSAKTESESSKKATDQEKVESLQNVGALTTKWQISHHYNPDVTTLLERDKYDAIQPEFLVKLSPEFLAKQREIDSIYYINKGSP----SPFIVWLYNQLNVEEPRSYSLKHDGPLCCLNVKNVNTEQGGSNSCKVINAAG-------DKTCSTHLKMKTAFSTLPIPT--------EEDSYATSLRLKYQTFELQPESQLFSESIKKKPEKPIFITKLSSECAALGETAKFTVIVSGFPKPIIQW 1566 HSP 2 Score: 70.4774 bits (171), Expect = 9.800e-12 Identity = 82/324 (25.31%), Postives = 129/324 (39.81%), Query Frame = 3
Query: 786 WFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIIT---KSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQ-LNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQ 1745
W KDG +L G + ++ + G L+I D+G Y+C A+NS+G T +C +T + KS LTE + + EN EE Y + ++++S+G + +I + K W NG + + + L I D G C +E G V+ + D +SA H+ PSF+ ++P Q +NEG +VT T ++ + S VEW K+ I S M++S + TL I +A EDSG + + A NK GQ
Sbjct: 26991 WMKDGKNLSQGGKYEIFEEHGSAHLEIYDADLSDSGVYTCTAQNSVGSVST-NCTLTIQARTTAAALEGKSELTEEIVKK----ENTYEEVQSYTEIKA-----------SKTQMTISQGQTVTLRASIPEASDVK----WILNGAELSNSESYRYGVSGSDHTLTIKSISHHDQGILTCEARTEQGVVKCQ-------------FD---MTVSATHS-------------------GSPSFL--VQPHSQNVNEGQNVTFTCEI--TGEPSPEVEWLKDNAVISITSNMKLSRSKNVYTLEIHNATIEDSGKFTVKAKNKFGQ 27255 HSP 3 Score: 67.3958 bits (163), Expect = 9.829e-11 Identity = 112/500 (22.40%), Postives = 180/500 (36.00%), Query Frame = 3
Query: 360 EINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSG----TAQSNIAHVRINP------------------ESGVDYLTQLPEEMMQEINNLDKNYETGTYHG------MEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNV-----TCHPEESIITKSNLTESSIS-QLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTL--TVKVEPANDNSLYVEWYKNENHIINGSRMQISYDR-GTATLNIVSAISEDSGDYWLIATNKNGQT 1748
EI W +G+ ++ R + G I L IL D G Y C K+ +G +AQ + NP ES Q E +Q + L ++ ++ +E DK+ A P+FL +L L + + + ++ S S + W + ++++ + +D C L++ E G SC N+ G ++C+ T I T+ + +S+ + ++ E E P P FI + ++ E A K T+ K WYHNG ++ + S T E +L I G Y C + G+ S S +H ++ K+ E I P FIK++E I Q G TV P D V WY+ NH+ I + G+ L ++ DSG Y ATN G+T
Sbjct: 1263 EIVWDHNGRTITNTERVQYAHEAGLISLIILNVHLGDQGSYCCTVKNRNGESRTSAQLTVEAKEANPKETPVPSDSISKSAKTESESSKKATDQEKVESLQNVGALTTKWQISHHYNPDVTTLLERDKYDA-IQPEFLVKLSPEF-LAKQREIDSIYYINKGSPSPFIV-WLYNQLNVEEPRSYSLKHDGPLCCLNVKNVNTEQGGSNSCKVINAAGD---KTCSTHLKMKTAFSTLPIPTEEDSYATSLRLKYQTFELQPESQLFSESIKKKPEKPIFITKLSSECAALGETA----KFTVIVSGFPKPIIQWYHNGNLINSSSFYTFVQEKEEHSLVITQITKELEGEYSCTASNRFGK---------------------STCTSYLHVKVKDIK------KQGETIGQPPYFIKEIEHI-QCAVGSQALFEYTVAGSPLPD----VHWYRGSNHMQPSEYCIIVNNADGSGYLKMLDIQQSDSGTYSCKATNPYGET 1720 HSP 4 Score: 63.929 bits (154), Expect = 1.010e-9 Identity = 110/556 (19.78%), Postives = 181/556 (32.55%), Query Frame = 3
Query: 363 INWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDN-GDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALD-IAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPRE-ISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEW 2021
+ W+KDGK L A R++T L+ L+ + G Y C + G +I H + SG +F+ L + +L QGQ L +E + + W+KDG R + C L+ + C G YSC N G CH ++ T+ + PP+F + P E I +EG K+ Q LS WY + + + T + N++ + + +D+G Y C +E G + V +N+ +K P+F L+P+ ++EG+ + L+ V + ++W K+ + + + ++++ GTATL I A D+GDY ATN G + AG N ++E P +I E G A F+ KV G P P ++W
Sbjct: 3222 VTWYKDGKQLYASYRYNTTVVGNTCTLECLHECTKETPGKYSCEVSNSYG---KDICHAEVTTFSGP---------------------------------------ARFVKTLTDQ-SLQQGQKLRLEASFK--GIGKIFVTWYKDGKQAYASYRCNTKVIGNTCVLEYLHECDENTPGKYSCEVMNEDGSD-------ICHALVTLGTERKI-----------------------------PPTFTK--KPSEHIEDAEGK--LVKLESRVSGSQPLSVIWYKDNKEINTSDIYDVSFKSNVAVMCLRKATVTDSGVYTCSASNEAGTASFQ-VVVNITEQKR-----------------------------------PPTFDIPLKPM-TVSEGERLNLSCHV--CGSPPVKIQWMKDRREVTSSASTKMTFVDGTATLEINQASKTDAGDYLCKATNNAG-------------------------NKAGVAPAAAPAAPTTPQATKKLDNLFFIEE---------PKSIHIVEKGTATFIAKVGGDPIPNVKW 3619 HSP 5 Score: 60.4622 bits (145), Expect = 1.282e-8 Identity = 115/536 (21.46%), Postives = 193/536 (36.01%), Query Frame = 3
Query: 672 TIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPE---------------ESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMP--------PSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSF--IKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLTVIPMVD 2204
T+P + P SLT+ +G+ + ++ S+ W ++ + R + +I D G+Y+ V NS GK E Q P ES KS S ++KS E P + R ++ P PSF + ++IS S A K+T+ K S W +G+ + + E + L I SD+G Y C + G V + TL +Q + ALE + + +K++ ++ S+ IK + +++G +VTL + A+D V+W N + N + TL I S D G I T + ++E+ + C F T P+F V+P + EG F +++G P+P +EW D+ ++ S K ++ LEI+ + K V A N G A+L V+ +V+
Sbjct: 26800 TLPATILTKPQSLTVSEGESVKFTCDVDGEPAPSVT--WMREAQIIVSSQRHLITSTEYKSTFEINKVEMSDGGNYTVVVENSEGKQEAQFSLTIRKPTPVQKVVASPPRVKSPESPRIKSPFGIRSPQRIKSPE-PIKSPTRVKSPPMVKSPTPKDKTSKPSFSTEL--KDISAS--ADTIVKLTVKLSGDLKPSISWMKDGKNLSQGGKYEIFEEHGSAHLEIYDADLSDSGVYTCTAQNSVGSVSTN-CTLTIQAR------------TTAAALEGKSELTEEIVKKENTYEEVQSYTEIKASKTQMTISQGQTVTLRASIPEASD----VKWILNGAELSNSESYRYGVSGSDHTLTIKSISHHDQG----ILT---CEARTEQGVVKC-----------------------------------QFDMTVSATHSGSPSFLVQPHSQNVNEGQNVTFTCEITGEPSPEVEWLKDNAVISITSNMKLSRSKNVYTLEIHNATIEDSGKFTVKAKNKFGQCSATASLNVLTLVE 27269 HSP 6 Score: 59.3066 bits (142), Expect = 2.648e-8 Identity = 99/461 (21.48%), Postives = 163/461 (35.36%), Query Frame = 3
Query: 363 INWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLK-LGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNG 1742
+ W KDG+ L RF D+ L I D G+Y VA + A + G D P E ++I ++K T + E++ I G+ W K+G+ ++ R + + R L I Y D G+Y+ +A GK + V+ H + IP P I + P +SV G PA F V + ++S WY N + + + + + E N L ++ P D Y C +++G V + +LNV E S ++ P++ A ++AP IIT L + EG+S +V + + L + WY+ + I+ Q++ T L+I A D+G+Y +A N G
Sbjct: 823 VMWMKDGQELELSERFRATTDKFLQRLMIQTVRMSDAGEYSVVAGASVSKANLVV--------EGKDVHISEPAE--KDITVVEKQRATFEFEVNEDE--------------------IDGR-------------------WQKNGVEIQSFDQRFNYVTVRKTHRLTITETYRSDAGEYTFIA----GKNRS---TVSLHVQ-----------------------------------IPEAPQIIRHMQP--LSVEAGKPARFSVEVTGIPQPQVS--WYKNSQALSSGFKCKFQREGNEHTLLLIEVFPEDAAQYNCEAKNDYG-VATSSASLNV--EVSEVVSPETV---------APVSAPV-------IITSLQD--------TSVEEGESARFQCRV---SGDDLKITWYRRDKEIVMSDNFQMTQFGDTCQLDISRAYVADAGEYTCVARNSGG 1158
BLAST of Titin vs. Ensembl Zebrafish
Match: obscnb (obscurin, cytoskeletal calmodulin and titin-interacting RhoGEF b [Source:ZFIN;Acc:ZDB-GENE-070119-5]) HSP 1 Score: 65.855 bits (159), Expect = 2.526e-10 Identity = 70/275 (25.45%), Postives = 118/275 (42.91%), Query Frame = 3
Query: 363 INWFKDGKLLSAGSRFSTIFDR-GFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQL-PEEMMQEIN-----NLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTID 1166
+ WFKDGKLL + D+ G L I + D G Y C+A++ SG A S A +R++ DY T E ++ + ++ +T + + ED+ +P+ + +L + L L G P+ ++ + + WFKDG L+ SRI + +R +L+I ED G+YS N G A + + ++ L E P + R D+ V +PP F+E ++ V +GA V ++
Sbjct: 823 VKWFKDGKLLEENDHYMINEDQEGRHQLIITTVLPTDMGVYRCMAENDSGIASSK-AELRVDMSCSSDYDTAADATETSSYVSAKGYVSSSEHRDTEAFESVAEDE----LLPQVVDEL-HDLFLSPGAPI-AKMSVRVKGFPTPRVYWFKDGTPLQASSRIVLSTERDQHSLEILDVKCEDTGEYSAYISNIAGSA--------------------YSSARMNVLAPGERPTPDQGRLRDSKVPLVPPRFLERFSNKK--VKQGASITLSVKVE 1068
BLAST of Titin vs. Ensembl Xenopus
Match: hao2 (hydroxyacid oxidase 2 (long chain) [Source:Xenbase;Acc:XB-GENE-979509]) HSP 1 Score: 60.8474 bits (146), Expect = 5.969e-9 Identity = 76/328 (23.17%), Postives = 119/328 (36.28%), Query Frame = 3
Query: 357 LEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVT-EWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHG 1337
+E++W+KDG L+ + T + L IL T G Y C A + GTA SN + +G+ Y + NY + E K+ P F P+ + + G P+ E H + S +T W KD +K G + L I D+G Y+C A N +GK +S ++LK +PPSF + + +V E FK+ Q L+ W N ++ S + NI L++ + PSD G Y C + ++ G
Sbjct: 138 IEVSWYKDGVLVRDDNNTQTSYIDNTATLQILQTDRHHAGQYSCTASNFVGTASSNAKLIL----TGLSYFA------------FNLNYLSYNLCKPLEGKN-----PPFFDVKPSPVDVPVGDPVKFEC---HVTGSQPITLTWTKDNKEVKSGGNYKITQLENTTQLTILKADKGDSGQYTCSATNDVGK-----------------------DSCTAKLKR-----------------KVPPSFTKKLSE---TVEETDGNSFKLEGRVAGSQPLTVAWLLNNNELQLSSNNEVTFKDNIVLLHVKKSSPSDAGLYTCKVSNDAG 398 HSP 2 Score: 53.9138 bits (128), Expect = 7.856e-7 Identity = 69/322 (21.43%), Postives = 118/322 (36.65%), Query Frame = 3
Query: 1068 SVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEWRIDD 2033
+ + PP FI P++P E++ + A ++ P ++ WY ++ + NI+ L +D+G YIC + G S +L+V+ K P PSF ++++ I E + + + + + V WYK+ + + + Q SY TATL I+ +G Y A+N G S + I+T S A + Y ++ + ++ + P FDVKP + G P KF V+G TL W D+
Sbjct: 7 AAVIQPPYFIAPLEPVEVTAGDAASLQCQIQGTP----EIKVSWYKGDTKLRPTDTQKILFKNNIARLVFAQVDVNDSGEYICKAENLVGEASSS-TSLSVKERK----------------------VP-------------PSFARKVKDI---QETVGLPILFECQIVGSEPIEVSWYKDGVLVRDDNNTQTSYIDNTATLQILQTDRHHAGQYSCTASNFVGTASS--------NAKLILTGLSYFAFNLNYL---------------SYNLCKPLEGKNPPFFDVKPSPVDVPVGDPVKFECHVTGSQPITLTWTKDN 262
BLAST of Titin vs. Ensembl Xenopus
Match: MYPN (myopalladin [Source:NCBI gene;Acc:100495211]) HSP 1 Score: 60.4622 bits (145), Expect = 8.267e-9 Identity = 60/209 (28.71%), Postives = 95/209 (45.45%), Query Frame = 3
Query: 1461 EMKEDEIITD---LPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHII---NGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQ--RLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLVVPDSVCK 2063
E++ DEI T P F ++L+ ++ EG SVT T KV +Y W+K+ I +MQ D GT L I +A ++D G+Y ++A N G+ ISC + LL +A ++ L ++ A + ++ + P F P ++ A EG + KVSG PTP + W ++ V PD+ K
Sbjct: 67 EVQHDEIPTGKCIAPIFDRRLKHF-RVVEGTSVTFTCKVVGIPIPKVY--WFKDGKQISKKDQHCKMQREAD-GTCALYIEAATNDDDGNYTIMAANPQGR-------ISC--------SGHLLVQNAPFRPRSLLPVQSHRARPRVPEVESEVVEERFFRPYFLQAPGDMVAHEGRLCRLDCKVSGLPTPDIMWLLNGRQVKPDATHK 256
BLAST of Titin vs. Ensembl Xenopus
Match: MYPN (myopalladin [Source:NCBI gene;Acc:100495211]) HSP 1 Score: 60.8474 bits (146), Expect = 1.026e-8 Identity = 67/242 (27.69%), Postives = 107/242 (44.21%), Query Frame = 3
Query: 1461 EMKEDEIITD---LPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHII---NGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQ--RLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLVVPDSVCKTY-NDGSINYLEINRCSELGDIKVHVAATNPLG 2159
E++ DEI T P F ++L+ ++ EG SVT T KV +Y W+K+ I +MQ D GT L I +A ++D G+Y ++A N G+ ISC + LL +A ++ L ++ A + ++ + P F P ++ A EG + KVSG PTP + W ++ V PD+ K + I+ L I+ ++ ATN G
Sbjct: 549 EVQHDEIPTGKCIAPIFDRRLKHF-RVVEGTSVTFTCKVVGIPIPKVY--WFKDGKQISKKDQHCKMQREAD-GTCALYIEAATNDDDGNYTIMAANPQGR-------ISC--------SGHLLVQNAPFRPRSLLPVQSHRARPRVPEVESEVVEERFFRPYFLQAPGDMVAHEGRLCRLDCKVSGLPTPDIMWLLNGRQVKPDATHKMLVRENGIHSLLIDPVTKADSGTYICIATNKTG 771
BLAST of Titin vs. Ensembl Xenopus
Match: ENSXETT00000050252.1 (pep primary_assembly:Xenopus_tropicalis_v9.1:9:58731943:58744919:1 gene:ENSXETG00000019973.1 transcript:ENSXETT00000050252.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 60.077 bits (144), Expect = 1.126e-8 Identity = 63/259 (24.32%), Postives = 108/259 (41.70%), Query Frame = 3
Query: 255 DSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSA----GSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQL 1019
+PTF Q ++S +VV EG ++ + E++WF+DG+++S G + S F G L I +G Y A + +G A S A + I A+T P +Q + T+ QG + +++ + + V +++++G ++ +I D +L IA YPED+G YS A N++G+A T + +T EE I K T S +Q+
Sbjct: 4 QAPTFIQPIQS-VVVLEGSTATFEAQI--SGFPVPEVSWFRDGQVISPATLPGVQIS--FSDGRAKLVIPTATAAHSGKYSLRAANGAGQATST-AELLIT----------------------------------------AETAPPNFTQRLQTTTVKQGSQVRLDVRVS--GIPTPVVKFYREGAEIRSSQDFQIIQDGDLYSLIIAEAYPEDSGTYSVNATNNVGRA-TSTAELTVQAEEEIPAKKTKTVVSTAQI 213
BLAST of Titin vs. Ensembl Xenopus
Match: MYPN (myopalladin [Source:NCBI gene;Acc:100495211]) HSP 1 Score: 59.6918 bits (143), Expect = 2.169e-8 Identity = 67/242 (27.69%), Postives = 107/242 (44.21%), Query Frame = 3
Query: 1461 EMKEDEIITD---LPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGS---RMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQ--RLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLVVPDSVCKTY-NDGSINYLEINRCSELGDIKVHVAATNPLG 2159
E++ DEI T P F ++L+ ++ EG SVT T KV +Y W+K+ I +MQ D GT L I +A ++D G+Y ++A N G+ ISC + LL +A ++ L ++ A + ++ + P F P ++ A EG + KVSG PTP + W ++ V PD+ K + I+ L I+ ++ ATN G
Sbjct: 877 EVQHDEIPTGKCIAPIFDRRLKHF-RVVEGTSVTFTCKVVGIPIPKVY--WFKDGKQISKKDQHCKMQREAD-GTCALYIEAATNDDDGNYTIMAANPQGR-------ISC--------SGHLLVQNAPFRPRSLLPVQSHRARPRVPEVESEVVEERFFRPYFLQAPGDMVAHEGRLCRLDCKVSGLPTPDIMWLLNGRQVKPDATHKMLVRENGIHSLLIDPVTKADSGTYICIATNKTG 1099 HSP 2 Score: 52.373 bits (124), Expect = 4.150e-6 Identity = 68/283 (24.03%), Postives = 117/283 (41.34%), Query Frame = 3
Query: 1284 TLPSDTGSYICVLFSE---HGRVESEPVTLNVQTEKSII--LDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTL---TVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNG-QTQSEKINIS--CFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNT--EDVQEEC------LPNFDVKPM-----------NITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLV 2042
TL D +Y C L + H E + + E + + +DP+ + +L+ E + + +E + P+FI++L+ ++ EG V L V P V WY + N +QI +L I A ED+G Y A+N G + S +I + F+ E + +++ YQ E ++ N+ T Q T + V++ C L D +P+ ++TA EG F +V G P+P +EW + L+
Sbjct: 182 TLEQDISNYTCQLENRERPHYTPEKTEINTTISEEANSLEAVDPECEK----ESLQVENDTQSSSFYFEEPVGRPPNFIQKLK-SREVTEGSKVQLDCIVVGFPPPE-----VRWYCEGKELENSPDIQIQKSGDLDSLVITEAFEEDTGRYSCFASNIYGTDSTSAEIYVEGMAFKPEPSAPDTQETSNADTYQ-----EMNIPPSNVQTMQPTMIQQVRKNCQSPTTYLQGLDGRPLIAAPVFTKSLQDLTACEGQLVVFESRVKGSPSPKVEWYREGTLI 449
BLAST of Titin vs. Ensembl Mouse
Match: Ttn (titin [Source:MGI Symbol;Acc:MGI:98864]) HSP 1 Score: 109.768 bits (273), Expect = 1.211e-23 Identity = 177/772 (22.93%), Postives = 302/772 (39.12%), Query Frame = 3
Query: 360 EINWFKDGKLLSAGSRFSTIF-DRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPE-EMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLS---------------------QL-----------PNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSL--KLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIV--KTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPI------DQLNEGD-----------------SVTLT--------VKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSA-GYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLTVIPMVDFRPDLRHI---TPE----NPFKKMAGLKKVEC---TSELNDAFKKSKPTAERIVKIEMGSET---KSKFYKSPDVIETEKLYDQVAA 2426
+I W+KDGK + G R+ F G L I + +D G Y A + G A + + + P + T +P E + I ++ + + M A+ P +S QL P S ++GQ ++ + T WF +G + ++ VI + G +L I P D+G+++ VA+N GK S I+ + E+ Q+K P+F+E + + +++ EG+ KV + W N +I+ R+ + +AL I + D+ Y ++ GR ++ +NV+ E +P+ E +L P+ + EI +LEP+ +Q EGD S+ L ++ P D ++ VEW + + +R+++ + G +L+ +A S DSG ATNK G + I + EK + S L D G QR+++LE G ++ T D +E+ P+ + P + EG A+F +V+G+P P + W ++ L+ + DG I+YL+I C +V V A NP GV + + L + DFR LR PE P K ++KV+ TSE + K + AERI ++ E+ +SKF + + E Y+ + A
Sbjct: 1277 KIAWYKDGKRIRHGERYQMDFLQDGRASLRIPVVLPEDEGIYTAFASNIKGNAICS-GKLYVEPAAPFSAPTYMPTPEAVSRIRSVSPRSLSRSPIRMSPAMSPARMSPARMSPARMSPARMSPGRRLEETDESQLERLYKPVFVLKPASFKCLEGQTARFDLKVVGRPMPE--TFWFHNGQQIVNDYTHKV-VIKEDGTQSLIIVPASPSDSGEWTVVAQNRAGK--------------STISVTLTVEAVEHQIK---------------------PAFVEKL--KNVNIKEGSRLEMKVRATGNPNPDIV--WLKNSDIIVPHKYPRIRIEGTRGEAALKIDSIISQDSAWYTATAINKAGR-DTTRCKVNVEVE---FAEPEP---------ERKLIIPRGTYRAKEIAA------PELEPLHLRYGQEQWEEGDLYDKEKQQKPFFKKKLTSLRLKRFGPAHFECRLTPIGDPTMVVEWLHDGKPLEAANRLRMINEFGYCSLDYGAAYSRDSGVITCRATNKYGTDHTSATLI--VKDEKSLVEESQLPDGKKGLQRIEELERMAHEGALTGV--TTDQKEKQKPDIVLFPEPVRVLEGETARFRCRVTGYPQPKVNWYLNGQLIRKSKRFRVRYDG-IHYLDIVDCKSYDTGEVKVTAENPEGVTEHKVKLEIQQREDFRSVLRRAPEPKPEFHVHEPGKLQFEVQKVDRPVDTSETKEVVKLKR--AERITHEKVSEESEELRSKFKRRTE----EGYYEAITA 1975 HSP 2 Score: 78.1814 bits (191), Expect = 4.984e-14 Identity = 146/693 (21.07%), Postives = 269/693 (38.82%), Query Frame = 3
Query: 357 LEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTA-----------------------QSNIAHVRINPES--------GVDYLTQLPEE----MMQEINNL----DKNYETGTYHGMEEDKHAAKTI-------PKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSII-------LDPQSKGISAIHALEAELNAPKAEMKED------EIITDLPS---------------FIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNF--DVKPMNITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLVVPDSVCKTY---NDGSINYLEINRCSELGDIKVHVA-ATNPLGVAKEEATLTVIP 2195
+ + W KDG+ L R ++ L I T D G Y VA + TA Q + +N + G++ Q+ E + + I+ + ++ + G Y + ++ T+ P+ L +L +T+ G+P + + W+K+ L G + ++D L + +PED Y+C A+N G A T + PE + +P +E Y PP+ + P+ ++ SEG PA F+ + +G L WY + +K + L I P D G+Y V + G+V S TL ++ +SI+ ++ + K I+++ + E + ++++ E++++ P+ FI+++ + +++ D L+V V ++W+ N + + + +D T +L I+ +D G+Y +A+N+ G K V ++ L G +R ++E SG E + C P F ++KP++ G G PA F V G P PT+ W +D+ + SVC T DGS ++ +N + GD +++ A N G + A L V+P
Sbjct: 3046 ITVQWMKDGQELQIADRIKIQKEKYVHRLLIPSTRMSDAGKYTVVAGGNMSTANLFVEGRDVRIRSIKKEVQVIEKQRAVVEFEVNEDDVDAHWYKDGIEINFQVQERHQYVVERRIHRMFISETRHSDAGEYTFVAGRNRSSVTLYVNAPEPPQVLQEL-QPVTVQSGKPARFCAVISGRPQPKI--SWYKEEQLLSTGFKCKFLHDGQEYTLLLIEAFPEDAAVYTCEAKNDYGVATTSASLSVEVPE-------------------VVSPDQEMPVY--------PPAIVTPL--QDTVTSEGRPARFQCQV---SGTDLKVSWYCKDKKIKPSRFFRMTQFEDTYQLEIAEAYPEDEGTYAFVANNAVGQVSST-ATLRLEAPESILHERIGQQIEMEMKEIASLLSAEEDFQTYSSDLRLPNANETLELLSEPPARSTQFDSRQEGAAPVFIREISDV-EISVEDVAKLSVTVTGCPKPK--IQWFFNGMLLTPSADYKFVFDGDTHSLIILFTRFQDEGEYTCLASNEYG---------------KAVCSAHLRISPRG-ERSTEME----SGEKKAL---EKPKGPCPPYFFKELKPVH--CGPGIPAVFEYSVHGEPAPTVLWFKEDMPLY-TSVCYTIIHSPDGSGTFI-VND-PQRGDSGLYLCKAQNLWGESTCAAELLVLP 3671 HSP 3 Score: 63.1586 bits (152), Expect = 2.019e-9 Identity = 82/315 (26.03%), Postives = 134/315 (42.54%), Query Frame = 3
Query: 294 VVDEGEPLRLMTRVGPADDGSLEINWF-KDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRIN-PESGV-DYLTQLPEEMMQEINNL---DKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQG-QPLHIEIFLEHHSDSSLVTE------------------WFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTI 1163
V EG P R +V D L+++W+ KD K+ + T F+ + L+I +D G Y VA + G S+ A +R+ PES + + + Q E M+EI +L +++++ TY +A +T+ + LS+ P T Q +F+ SD + E WF +GM L + ++D +L I F +D G+Y+C+A N GKA S ++ P TE + K+LE P P PP F + + P + G PA F+ ++
Sbjct: 3315 VTSEGRPARFQCQVSGTD---LKVSWYCKDKKIKPSRFFRMTQFEDTY-QLEIAEAYPEDEGTYAFVANNAVGQV-SSTATLRLEAPESILHERIGQQIEMEMKEIASLLSAEEDFQ--TYSSDLRLPNANETL-ELLSEPPARSTQFDSRQEGAAPVFIREISDVEISVEDVAKLSVTVTGCPKPKIQWFFNGMLLTPSADYKFVFDGDTHSLIILFTRFQDEGEYTCLASNEYGKA-VCSAHLRISPR-----GERSTEMESGEKKALEKPKG-----------PCPPYFFKELKP--VHCGPGIPAVFEYSV 3602 HSP 4 Score: 60.077 bits (144), Expect = 1.906e-8 Identity = 63/281 (22.42%), Postives = 119/281 (42.35%), Query Frame = 3
Query: 108 YNNNSNFNSVPDEGVIPNGTTMAPHALEVVNQVNKRSLASPR--RGSDHLYDSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYE---TGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDR--GYCALDIAFCYPEDNGDYSCVARNSLGK 929
Y + + + GV +G+T+ EV+ + + R ++ PT L++ V+ EGE + L + + S ++ W+++ + + F F G L I +D+G + C A + +GT ++ ++ + D T L E+ E ++ + T T E+ P F+S+ P L++G + E + + + W K G+ L G R V Y++ G C L I+ + +D G+Y+ V RN G+
Sbjct: 847 YKSQAGIEVKKEVGVSISGSTVREEHFEVLRGREAKVTETARVPAPAEVPVTPPTLVSGLKNVTVI-EGESVTLECHI--SGYPSPKVTWYREDYQIESSIDFQITFQGGIARLMIREAFAEDSGRFTCSAVNEAGTVSTS-CYLAVQVSEEFDKETTLTEKFATEEKRFVESRDVVMTDTSITEEQAGPGEPAAPFFISK-PVVQKLVEGGSVVFECQIGGNPKPHVY--WKKSGVPLTTGYRYKVSYNKQTGECRLVISMTFADDAGEYTIVIRNKHGE 1120 HSP 5 Score: 57.7658 bits (138), Expect = 8.414e-8 Identity = 78/331 (23.56%), Postives = 136/331 (41.09%), Query Frame = 3
Query: 978 ITKSNLTESSISQLKSLENPGEEYARYNDTSVIPM-PPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITD---------LPSFIKQLEPIDQ-LNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAIS--EDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRL-KDLEEDLASGNISTFQNTEDVQEECLPNF 1928
I+ S + E L+ E E AR + +P+ PP+ + + + ++V EG + I K++ WY +++ + I+ L I D+G + C +E G V S L VQ + D ++ E + + D IT+ P FI + P+ Q L EG SV ++ + +V W K+ + G R ++SY++ T +V +++ +D+G+Y ++ NK+G+T +AS+ L + A Y+ L K +E L +STF V E P F
Sbjct: 863 ISGSTVREEHFEVLRGREAKVTETARVPAPAEVPVTPPTLVSGL--KNVTVIEGESVTLECHISGYPSPKVT--WYREDYQIESSIDFQITFQGGIARLMIREAFAEDSGRFTCSAVNEAGTV-STSCYLAVQVSEE--FDKETTLTEKFATEEKRFVESRDVVMTDTSITEEQAGPGEPAAPFFISK--PVVQKLVEGGSVVFECQI--GGNPKPHVYWKKSGVPLTTGYRYKVSYNKQTGECRLVISMTFADDAGEYTIVIRNKHGET----------------SASASLLEEADYEALVKTQQEMLYQTQMSTFIQEPKVG-EIAPGF 1165 HSP 6 Score: 56.6102 bits (135), Expect = 1.695e-7 Identity = 57/223 (25.56%), Postives = 95/223 (42.60%), Query Frame = 3
Query: 360 EINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLH----IEIFLEH--HSDSSLVTEWFKDGMSLKLGSRIDVIYD-RGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESS 1007
+I WF +G LL+ + + +FD L IL+T D G+Y C+A + G A + AH+RI+P + EM E+G +E+ K P F +L +P+H I E+ H + + WFK+ M L +I+ G + D+G Y C A+N G++ T + + PE++ + ++ E S
Sbjct: 3493 KIQWFFNGMLLTPSADYKFVFDGDTHSLIILFTRFQDEGEYTCLASNEYGKAVCS-AHLRISPRG------ERSTEM-----------ESGEKKALEKPKGPCP--PYFFKEL---------KPVHCGPGIPAVFEYSVHGEPAPTVLWFKEDMPLYTSVCYTIIHSPDGSGTFIVNDPQRGDSGLYLCKAQNLWGES-TCAAELLVLPEDTDVPDASCKEES 3685 HSP 7 Score: 56.6102 bits (135), Expect = 2.023e-7 Identity = 64/258 (24.81%), Postives = 107/258 (41.47%), Query Frame = 3
Query: 258 SPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSA----GSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQL 1019
+P F Q L+S +VV EG V + E++WF+DG+++S G + S F G L I + ++G Y A + SG A S E++ A+T P SQ S+T+ QG + +++ + + V ++++DG ++ + + +L IA YPED+G YS A NS+G+A T + + EE + K T S +Q+
Sbjct: 5 APMFTQPLQS-VVVLEGSTATFEAHV--SGSPVPEVSWFRDGQVISTSTLPGVQIS--FSDGRARLMIPAVTKANSGRYSLRATNGSGQATST-------------------AELLV----------------------TAETAPPNFSQRLQSMTVRQGSQVRLQVRV--TGIPTPVVKFYRDGAEIQSSLDFQISQEGDLYSLLIAEAYPEDSGTYSVNATNSVGRA-TSTAELVVQGEEVVPAKKTKTIVSTAQI 213 HSP 8 Score: 54.6842 bits (130), Expect = 7.051e-7 Identity = 48/187 (25.67%), Postives = 81/187 (43.32%), Query Frame = 3
Query: 1476 EIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEG----EKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEWR 2024
E+ P+ + L+ + + EG+SVTL + + S V WY+ + I + QI++ G A L I A +EDSG + A N+ G ++ SC+ E+ ++L A ++ D+ + S + E P F KP+ EGG F ++ G P P + W+
Sbjct: 894 EVPVTPPTLVSGLKNVTVI-EGESVTLECHI--SGYPSPKVTWYREDYQIESSIDFQITFQGGIARLMIREAFAEDSGRFTCSAVNEAGT-----VSTSCYLAVQVSEEFDKETTLTEKFATEEKRFVESRDVVMTDTSITEEQAGPGEPAAPFFISKPVVQKLVEGGSVVFECQIGGNPKPHVYWK 1072
BLAST of Titin vs. Ensembl Mouse
Match: Ttn (titin [Source:MGI Symbol;Acc:MGI:98864]) HSP 1 Score: 109.383 bits (272), Expect = 1.229e-23 Identity = 177/772 (22.93%), Postives = 302/772 (39.12%), Query Frame = 3
Query: 360 EINWFKDGKLLSAGSRFSTIF-DRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPE-EMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLS---------------------QL-----------PNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSL--KLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIV--KTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPI------DQLNEGD-----------------SVTLT--------VKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLAD-SAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLTVIPMVDFRPDLRHI---TPE----NPFKKMAGLKKVEC---TSELNDAFKKSKPTAERIVKIEMGSET---KSKFYKSPDVIETEKLYDQVAA 2426
+I W+KDGK + G R+ F G L I + +D G Y A + G A + + + P + T +P E + I ++ + + M A+ P +S QL P S ++GQ ++ + T WF +G + ++ VI + G +L I P D+G+++ VA+N GK S I+ + E+ Q+K P+F+E + + +++ EG+ KV + W N +I+ R+ + +AL I + D+ Y ++ GR ++ +NV+ E +P+ E +L P+ + EI +LEP+ +Q EGD S+ L ++ P D ++ VEW + + +R+++ + G +L+ +A S DSG ATNK G + I + EK + S L D G QR+++LE G ++ T D +E+ P+ + P + EG A+F +V+G+P P + W ++ L+ + DG I+YL+I C +V V A NP GV + + L + DFR LR PE P K ++KV+ TSE + K + AERI ++ E+ +SKF + + E Y+ + A
Sbjct: 1277 KIAWYKDGKRIRHGERYQMDFLQDGRASLRIPVVLPEDEGIYTAFASNIKGNAICS-GKLYVEPAAPFSAPTYMPTPEAVSRIRSVSPRSLSRSPIRMSPAMSPARMSPARMSPARMSPARMSPGRRLEETDESQLERLYKPVFVLKPASFKCLEGQTARFDLKVVGRPMPE--TFWFHNGQQIVNDYTHKV-VIKEDGTQSLIIVPASPSDSGEWTVVAQNRAGK--------------STISVTLTVEAVEHQIK---------------------PAFVEKL--KNVNIKEGSRLEMKVRATGNPNPDIV--WLKNSDIIVPHKYPRIRIEGTRGEAALKIDSIISQDSAWYTATAINKAGR-DTTRCKVNVEVE---FAEPEP---------ERKLIIPRGTYRAKEIAAP------ELEPLHLRYGQEQWEEGDLYDKEKQQKPFFKKKLTSLRLKRFGPAHFECRLTPIGDPTMVVEWLHDGKPLEAANRLRMINEFGYCSLDYGAAYSRDSGVITCRATNKYGTDHTSATLI--VKDEKSLVEESQLPDGKKGLQRIEELERMAHEGALTGV--TTDQKEKQKPDIVLFPEPVRVLEGETARFRCRVTGYPQPKVNWYLNGQLIRKSKRFRVRYDG-IHYLDIVDCKSYDTGEVKVTAENPEGVTEHKVKLEIQQREDFRSVLRRAPEPKPEFHVHEPGKLQFEVQKVDRPVDTSETKEVVKLKR--AERITHEKVSEESEELRSKFKRRTE----EGYYEAITA 1975 HSP 2 Score: 58.5362 bits (140), Expect = 4.466e-8 Identity = 64/281 (22.78%), Postives = 118/281 (41.99%), Query Frame = 3
Query: 108 YNNNSNFNSVPDEGVIPNGTTMAPHALEVVNQVNKRSLASPR--RGSDHLYDSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYE---TGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDR--GYCALDIAFCYPEDNGDYSCVARNSLGK 929
Y + + + GV +G+T+ EV+ + + R ++ PT L++ V+ EGE + L + + S ++ W+++ + + F F G L I +D+G + C A + +GT S ++ + D T L E+ E ++ + T T E+ P F+S+ P L++G + E + + + W K G+ L G R V Y++ G C L I+ + +D G+Y+ V RN G+
Sbjct: 847 YKSQAGIEVKKEVGVSISGSTVREEHFEVLRGREAKVTETARVPAPAEVPVTPPTLVSGLKNVTVI-EGESVTLECHI--SGYPSPKVTWYREDYQIESSIDFQITFQGGIARLMIREAFAEDSGRFTCSAVNEAGTV-STSCYLAVQVSEEFDKETTLTEKFATEEKRFVESRDVVMTDTSITEEQAGPGEPAAPFFISK-PVVQKLVEGGSVVFECQIGGNPKPHVY--WKKSGVPLTTGYRYKVSYNKQTGECRLVISMTFADDAGEYTIVIRNKHGE 1120 HSP 3 Score: 58.151 bits (139), Expect = 7.167e-8 Identity = 78/331 (23.56%), Postives = 136/331 (41.09%), Query Frame = 3
Query: 978 ITKSNLTESSISQLKSLENPGEEYARYNDTSVIPM-PPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITD---------LPSFIKQLEPIDQ-LNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAIS--EDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRL-KDLEEDLASGNISTFQNTEDVQEECLPNF 1928
I+ S + E L+ E E AR + +P+ PP+ + + + ++V EG + I K++ WY +++ + I+ L I D+G + C +E G V S L VQ + D ++ E + + D IT+ P FI + P+ Q L EG SV ++ + +V W K+ + G R ++SY++ T +V +++ +D+G+Y ++ NK+G+T +AS+ L + A Y+ L K +E L +STF V E P F
Sbjct: 863 ISGSTVREEHFEVLRGREAKVTETARVPAPAEVPVTPPTLVSGL--KNVTVIEGESVTLECHISGYPSPKVT--WYREDYQIESSIDFQITFQGGIARLMIREAFAEDSGRFTCSAVNEAGTV-STSCYLAVQVSEE--FDKETTLTEKFATEEKRFVESRDVVMTDTSITEEQAGPGEPAAPFFISK--PVVQKLVEGGSVVFECQI--GGNPKPHVYWKKSGVPLTTGYRYKVSYNKQTGECRLVISMTFADDAGEYTIVIRNKHGET----------------SASASLLEEADYEALVKTQQEMLYQTQMSTFIQEPKVG-EIAPGF 1165 HSP 4 Score: 56.225 bits (134), Expect = 2.722e-7 Identity = 64/258 (24.81%), Postives = 107/258 (41.47%), Query Frame = 3
Query: 258 SPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSA----GSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQL 1019
+P F Q L+S +VV EG V + E++WF+DG+++S G + S F G L I + ++G Y A + SG A S A + + A+T P SQ S+T+ QG + +++ + + V ++++DG ++ + + +L IA YPED+G YS A NS+G+A T + + EE + K T S +Q+
Sbjct: 5 APMFTQPLQS-VVVLEGSTATFEAHV--SGSPVPEVSWFRDGQVISTSTLPGVQIS--FSDGRARLMIPAVTKANSGRYSLRATNGSGQATST-AELLVT----------------------------------------AETAPPNFSQRLQSMTVRQGSQVRLQVRVT--GIPTPVVKFYRDGAEIQSSLDFQISQEGDLYSLLIAEAYPEDSGTYSVNATNSVGRA-TSTAELVVQGEEVVPAKKTKTIVSTAQI 213 HSP 5 Score: 55.4546 bits (132), Expect = 3.657e-7 Identity = 49/191 (25.65%), Postives = 82/191 (42.93%), Query Frame = 3
Query: 1476 EIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEG----EKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEWRIDDV 2036
E+ P+ + L+ + + EG+SVTL + + S V WY+ + I + QI++ G A L I A +EDSG + A N+ G ++ SC+ E+ ++L A ++ D+ + S + E P F KP+ EGG F ++ G P P + W+ V
Sbjct: 894 EVPVTPPTLVSGLKNVTVI-EGESVTLECHI--SGYPSPKVTWYREDYQIESSIDFQITFQGGIARLMIREAFAEDSGRFTCSAVNEAG-----TVSTSCYLAVQVSEEFDKETTLTEKFATEEKRFVESRDVVMTDTSITEEQAGPGEPAAPFFISKPVVQKLVEGGSVVFECQIGGNPKPHVYWKKSGV 1076 HSP 6 Score: 54.6842 bits (130), Expect = 7.820e-7 Identity = 80/391 (20.46%), Postives = 141/391 (36.06%), Query Frame = 3
Query: 357 LEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTA-----------------------QSNIAHVRINPES--------GVDYLTQLPEE----MMQEINNL----DKNYETGTYHGMEEDKHAAKTI-------PKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSII 1391
+ + W KDG+ L R ++ L I T D G Y VA + TA Q + +N + G++ Q+ E + + I+ + ++ + G Y + ++ T+ P+ L +L +T+ G+P + + W+K+ L G + ++D L + +PED Y+C A+N G A T + PE + +P +E Y PP+ + P+ ++ SEG PA F+ + +G L WY + +K + L I P D G+Y V + G+V S TL ++ +SI+
Sbjct: 3046 ITVQWMKDGQELQIADRIKIQKEKYVHRLLIPSTRMSDAGKYTVVAGGNMSTANLFVEGRDVRIRSIKKEVQVIEKQRAVVEFEVNEDDVDAHWYKDGIEINFQVQERHQYVVERRIHRMFISETRHSDAGEYTFVAGRNRSSVTLYVNAPEPPQVLQEL-QPVTVQSGKPARFCAVISGRPQPKI--SWYKEEQLLSTGFKCKFLHDGQEYTLLLIEAFPEDAAVYTCEAKNDYGVATTSASLSVEVPE-------------------VVSPDQEMPVY--------PPAIVTPL--QDTVTSEGRPARFQCQV---SGTDLKVSWYCKDKKIKPSRFFRMTQFEDTYQLEIAEAYPEDEGTYAFVANNAVGQVSST-ATLRLEAPESIL 3400
BLAST of Titin vs. Ensembl Mouse
Match: Ttn (titin [Source:MGI Symbol;Acc:MGI:98864]) HSP 1 Score: 109.768 bits (273), Expect = 1.258e-23 Identity = 177/772 (22.93%), Postives = 302/772 (39.12%), Query Frame = 3
Query: 360 EINWFKDGKLLSAGSRFSTIF-DRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPE-EMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLS---------------------QL-----------PNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSL--KLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIV--KTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPI------DQLNEGD-----------------SVTLT--------VKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLAD-SAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLTVIPMVDFRPDLRHI---TPE----NPFKKMAGLKKVEC---TSELNDAFKKSKPTAERIVKIEMGSET---KSKFYKSPDVIETEKLYDQVAA 2426
+I W+KDGK + G R+ F G L I + +D G Y A + G A + + + P + T +P E + I ++ + + M A+ P +S QL P S ++GQ ++ + T WF +G + ++ VI + G +L I P D+G+++ VA+N GK S I+ + E+ Q+K P+F+E + + +++ EG+ KV + W N +I+ R+ + +AL I + D+ Y ++ GR ++ +NV+ E +P+ E +L P+ + EI +LEP+ +Q EGD S+ L ++ P D ++ VEW + + +R+++ + G +L+ +A S DSG ATNK G + I + EK + S L D G QR+++LE G ++ T D +E+ P+ + P + EG A+F +V+G+P P + W ++ L+ + DG I+YL+I C +V V A NP GV + + L + DFR LR PE P K ++KV+ TSE + K + AERI ++ E+ +SKF + + E Y+ + A
Sbjct: 1323 KIAWYKDGKRIRHGERYQMDFLQDGRASLRIPVVLPEDEGIYTAFASNIKGNAICS-GKLYVEPAAPFSAPTYMPTPEAVSRIRSVSPRSLSRSPIRMSPAMSPARMSPARMSPARMSPARMSPGRRLEETDESQLERLYKPVFVLKPASFKCLEGQTARFDLKVVGRPMPE--TFWFHNGQQIVNDYTHKV-VIKEDGTQSLIIVPASPSDSGEWTVVAQNRAGK--------------STISVTLTVEAVEHQIK---------------------PAFVEKL--KNVNIKEGSRLEMKVRATGNPNPDIV--WLKNSDIIVPHKYPRIRIEGTRGEAALKIDSIISQDSAWYTATAINKAGR-DTTRCKVNVEVE---FAEPEP---------ERKLIIPRGTYRAKEIAAP------ELEPLHLRYGQEQWEEGDLYDKEKQQKPFFKKKLTSLRLKRFGPAHFECRLTPIGDPTMVVEWLHDGKPLEAANRLRMINEFGYCSLDYGAAYSRDSGVITCRATNKYGTDHTSATLI--VKDEKSLVEESQLPDGKKGLQRIEELERMAHEGALTGV--TTDQKEKQKPDIVLFPEPVRVLEGETARFRCRVTGYPQPKVNWYLNGQLIRKSKRFRVRYDG-IHYLDIVDCKSYDTGEVKVTAENPEGVTEHKVKLEIQQREDFRSVLRRAPEPKPEFHVHEPGKLQFEVQKVDRPVDTSETKEVVKLKR--AERITHEKVSEESEELRSKFKRRTE----EGYYEAITA 2021 HSP 2 Score: 80.1073 bits (196), Expect = 1.455e-14 Identity = 152/705 (21.56%), Postives = 282/705 (40.00%), Query Frame = 3
Query: 219 LASPRRGSDHLY----DSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPE-----ESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLE--MNISALNILYTLPSDTGSYICVLFSEHGRVE-SEPVTLNVQTEKSII-------LDPQSKGISAIHALEAELNAPKAEMKED------EIITDLPS---------------FIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNF--DVKPMNITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLVVPDSVCKTY---NDGSINYLEINRCSELGDIKVHVA-ATNPLGVAKEEATLTVIP 2195
+A R S LY + P Q+L+ + V G+P R + + +I+W+K+ +LLS G + + D L ++ +D Y C AK+ G A ++ + ++PE + D+ P ++ L +++T +G+P F S + L W+ +K + L+IA YPED G Y+ VA N++G+ S T E E + +KS S LK E G++ P FI+P+ + E + +V+ P K + W+HN + + + E ++ + I+ P G Y C + G + +T+ + +SI+ ++ + K I+++ + E + ++++ E++++ P+ FI+++ + +++ D L+V V ++W+ N + + + +D T +L I+ +D G+Y +A+N+ G K V ++ L G +R ++E SG E + C P F ++KP++ G G PA F V G P PT+ W +D+ + SVC T DGS ++ +N + GD +++ A N G + A L V+P
Sbjct: 3227 VAGRNRSSVTLYVNAPEPPQVLQELQP-VTVQSGKPARFCAVI--SGRPQPKISWYKEEQLLSTGFKCKFLHDGQEYTLLLIEAFPEDAAVYTCEAKNDYGVATTSAS-----------LSVEVPEVV-------------------SPDQEMPVYPPAIVTPLQDTVTS-EGRPAR---FQCQVSGTDLKVSWYCKDKKIKPSRFFRMTQFEDTYQLEIAEAYPEDEGTYAFVANNAVGQV---SSTATLRLEGFSKSEEVTSKSQWHTSPSVSLKK-EALGQK-------------PIFIQPLSSLRVHSGETVRFHARVSGVP----KPAIQWFHNHQPILPSKDVVFHFEESTGMALMLIVDAYPEHAGKYSCRAANSAGEATCAATLTVTAKAPESILHERIGQQIEMEMKEIASLLSAEEDFQTYSSDLRLPNANETLELLSEPPARSTQFDSRQEGAAPVFIREISDV-EISVEDVAKLSVTVTGCPKPK--IQWFFNGMLLTPSADYKFVFDGDTHSLIILFTRFQDEGEYTCLASNEYG---------------KAVCSAHLRISPRG-ERSTEME----SGEKKAL---EKPKGPCPPYFFKELKPVH--CGPGIPAVFEYSVHGEPAPTVLWFKEDMPLY-TSVCYTIIHSPDGSGTFI-VND-PQRGDSGLYLCKAQNLWGESTCAAELLVLP 3842 HSP 3 Score: 79.7221 bits (195), Expect = 1.638e-14 Identity = 144/688 (20.93%), Postives = 256/688 (37.21%), Query Frame = 3
Query: 357 LEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTA-----------------------QSNIAHVRINPES--------GVDYLTQLPEE----MMQEINNL----DKNYETGTYHGMEEDKHAAKTI-------PKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELN---APKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDR--GTATLNIVSAISEDSGDYWLIATNKNGQ-TQSEKINISCFEGEKIV-------------TASSLLADSAGYQ------RLKDLEE--DLASGNISTFQNTEDVQEECLPNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLVVPDSVCKTYNDGSINYLEI--NRCSELGDIKVHVAATNPLGVAKEEATLTVIP 2195
+ + W KDG+ L R ++ L I T D G Y VA + TA Q + +N + G++ Q+ E + + I+ + ++ + G Y + ++ T+ P+ L +L +T+ G+P + + W+K+ L G + ++D L + +PED Y+C A+N G A T + PE + +P +E Y PP+ + P+ ++ SEG PA F+ + +G L WY + +K + L I P D G+Y V + G+V S TL ++ G S + ++ +P +K+ E + P FI+ L + +++ G++V +V + ++W+ N I+ + ++ G A + IV A E +G Y A N G+ T + + ++ E I+ +SLL+ +Q RL + E +L S + + QE P F + ++ AK V V+G P P ++W + +L+ P + K DG + L I R + G+ A+N G A A L + P
Sbjct: 3092 ITVQWMKDGQELQIADRIKIQKEKYVHRLLIPSTRMSDAGKYTVVAGGNMSTANLFVEGRDVRIRSIKKEVQVIEKQRAVVEFEVNEDDVDAHWYKDGIEINFQVQERHQYVVERRIHRMFISETRHSDAGEYTFVAGRNRSSVTLYVNAPEPPQVLQEL-QPVTVQSGKPARFCAVISGRPQPKI--SWYKEEQLLSTGFKCKFLHDGQEYTLLLIEAFPEDAAVYTCEAKNDYGVATTSASLSVEVPE-------------------VVSPDQEMPVY--------PPAIVTPL--QDTVTSEGRPARFQCQV---SGTDLKVSWYCKDKKIKPSRFFRMTQFEDTYQLEIAEAYPEDEGTYAFVANNAVGQVSST-ATLRLE------------GFSKSEEVTSKSQWHTSPSVSLKK-EALGQKPIFIQPLSSL-RVHSGETVRFHARVSGVPKPA--IQWFHNHQPILPSKDVVFHFEESTGMALMLIVDAYPEHAGKYSCRAANSAGEATCAATLTVTAKAPESILHERIGQQIEMEMKEIASLLSAEEDFQTYSSDLRLPNANETLELLSEPPARSTQFDSRQEGAAPVFIREISDVEISVEDVAKLSVTVTGCPKPKIQWFFNGMLLTPSADYKFVFDGDTHSLIILFTRFQDEGEYT--CLASNEYGKAVCSAHLRISP 3725 HSP 4 Score: 76.6406 bits (187), Expect = 1.367e-13 Identity = 78/355 (21.97%), Postives = 130/355 (36.62%), Query Frame = 3
Query: 678 PKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNG 1742
P + + P +T+ G P +E + + L +WFKDG L GSR + + R +L I D G Y+ N +GK+ SC V+ H + ++ PPSF+ + ++ S + GA + + +S W+ +G + + + N+ L + PSDTG+Y CV + G+ ES V L VQ + PSF + + ++ L G S+T T + V+W+K +++G IS + L ++ SGDY + TN G
Sbjct: 7657 PATIVEKPEPMTVTTGNPFTLECVVAGTPE--LSAKWFKDGRELSSGSRHHITFVRNLASLKIPSAEMNDKGLYTFEVENRVGKS---SCTVSVHVSDRVV----------------------------------PPSFVRRL--KDTSATLGASVVLECRV--SGSAPISVGWFLDGNEIISSPKCQSSFADNVCTLTLSSLEPSDTGAYTCVAANVAGQDESSAV-LTVQ--------------------------------------EPPSFEQTPDSVEVL-PGMSLTFTSVIR--GTPPFKVKWFKGSRELVSGEACTISLEDFVTELELLEVEPGQSGDYSCLVTNDAG 7926 HSP 5 Score: 71.633 bits (174), Expect = 4.915e-12 Identity = 118/561 (21.03%), Postives = 202/561 (36.01%), Query Frame = 3
Query: 180 HALEVVN-QVNKRSL----ASPRRGSD------HLYDSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAG 1829
H+LE+ N V R + AS + G+D + D P F ++LE+ V ++ +D + I W KDG+ L AG + F+ L+I D+G Y C A + +G++ S+ A P P F+ ++ S ++ G+ + L+ + WFK+ L + + + + LDI +D+G YSC A N +G + SC+ VI PPSFI+ ++P +I GA A + I G G +W+ + + +++ + + L I +D G Y CV+ +E G+ G A H L+ + P+F+K+++ L G +VTL V + V W K + I ++++S+ G A L I G Y +A N+ G +Q+ + E KI+ + L+ +AG
Sbjct: 4619 HSLELSNLTVQDRGIYSCKASNKFGADICQAELTIIDKPHFIKELEA---VQSAINKKIHLECQVDEDRKVTITWSKDGQKLPAGKDYKIYFEDKIASLEIPLAKLKDSGTYTCTASNEAGSSSSSAAVAVREP-------------------------------------------PSFVKKVDPSYLMLPGESARLHCKLK--GSPVIQVTWFKNNKELSESNTVRMSFVNSEAILDITDVKVDDSGTYSCEATNDVG---SDSCSTEV-------------------------------------VIKEPPSFIKTLEPADI--VRGANALLQCEIA-GTGP-FEVNWFKDKKQIRSSKKYRLFTQKTFVYLEISSFNSADVGDYECVVANEVGKC----------------------GCVATHLLK-----------------EPPTFVKKVDDFTALA-GQTVTLQAAVR--GSEPISVMWMKGQEVIKEDGKIKMSFSNGVAVLTIPDVQISLGGKYTCLAENEAG-SQTSVGELIVKEPAKIIERAELIQVTAG 5044 HSP 6 Score: 71.633 bits (174), Expect = 5.771e-12 Identity = 101/514 (19.65%), Postives = 178/514 (34.63%), Query Frame = 3
Query: 357 LEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPE---------------------------EMMQEINNLDKNYE---------------TGTY-------HGMEEDKHAAKTI-PKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVE-SEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQ 1745
+++W KD + L +G ++ + + + IL D G+Y C A + G+ + P V LT + E+++E +N+ +Y G Y GM+E + P + + P S+ + G +E + + L T+WFKDG L ++ + + L I P D+G YS +N +GK SC V+ + II PPSF + +E + G+ + + +S W+H+G + + + L N AL + +D G Y C+ + G E S P+T + + PSF+++ +P+D L G +VT T V+ V W+K ++ G+R +S L + + SG+Y I +N+ G+
Sbjct: 8441 FQVSWHKDKRELRSGKKYKIMSENLLTSIHILNVDTADIGEYQCKATNDVGSDTCVGSVTMKAPPQFVKKLTDISTIIGKEVQLQTTIEGAEPISVAWFKDKGEIVRESDNIWISYSENIATLQFSRAEPANAGKYTCQIKNDAGMQECYATLSVLEPAAIVEKPESIKVTTGDTCTLECTVSGTPE--LSTKWFKDGKELTSDNKYKISFFNKVSGLKIINVVPGDSGVYSFEVQNPVGK---DSCKVSIQVSDRII----------------------------------PPSFTRKL--KETNGLSGSSVVMECKV--YGSPPISVLWFHDGNEISSGRKYQTTLTDNTCALTVNMLEEADAGDYTCIATNVAGSDECSAPLT----------------------------------------VREPPSFVQKPDPMDVLT-GSNVTFTSIVK--GSPPFTVSWFKGSTELVPGARCNVSLQDSVGELELFDVDTSQSGEYTCIVSNEAGR 8868 HSP 7 Score: 68.1662 bits (165), Expect = 6.245e-11 Identity = 112/567 (19.75%), Postives = 190/567 (33.51%), Query Frame = 3
Query: 42 RHKYSFIFNLDFIAMTTQESMEYNNNSNFNSVPDEGVIPNGTTMAPHALEVVNQVNKRSLASPRRGSDHLYDSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNG 1742
+HK+SF N F+ ++ E GT + N+ + G + + P F + +S+ V + G ++L VG + I WFKD K L G+ S D +L++ D+G Y+C + GT S P P+F+ + L L GQ E + + + W+ DG + + + + G I+ E++G Y C ARN G T SC++ +LK E PP FI ++P E+ +V W N + +++ + T +M++ L+I PSD G Y C++ +E G S SA AL+ + PSFIK++E + + + + T + A + + W K++ + + IS++ ATL + S + SG Y A N++G
Sbjct: 5733 KHKFSFHDNTAFLEISQLE---------------------GTDSGTYTCSATNKAGHSQCS----GHLTVKEPPYFVEKPQSQDV-NPGTRVQLKALVG--GTAPMTIKWFKDNKELHPGAARSVWKDDTSTILELFSAKAADSGTYICQLSNDVGTTSSKATIFVKEP-------------------------------------------PQFIKKPSPVLVLRNGQSTTFECQVTGTPE--IRVSWYLDGNEITDLRKYGISFVDGLATFQISNARVENSGTYVCEARNDAG---TASCSI--------------------ELKVKE-----------------PPIFIRELEPVEVVKDSDVELECEVM----GTTPFEVTWLKNNKEIRSGKKYTMSEKMSVFYLHITKCDPSDVGEYQCIIANEGG----------------------SCACSARVALK-----------------EPPSFIKKIENVTTVLKSSA---TFQSTVAGSPPISITWLKDDQILEENDNVHISFEDSVATLQVRSVDNGHSGRYTCQAKNESG 6140 HSP 8 Score: 68.1662 bits (165), Expect = 6.245e-11 Identity = 108/532 (20.30%), Postives = 176/532 (33.08%), Query Frame = 3
Query: 357 LEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIA---HVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREI---------SVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRV------------------------ESEPVTLN----------------------------VQTEKSIILDPQSKGI--SAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQS 1754
L WFKDGK L++ +++ F L I+ + D+G Y ++ G ++ RI P S L +E N L +G+ ME + G P + WF DG + G + CAL + D GDY+C+A N G E C+ E PPSF++ DP ++ S+ +G+P + + W+ + +R L+ ++ L + S +G Y C++ +E GR + +P+ L TEKS IL+ S + S + E + K +I + P F+KQLEP+ ++ GDS +L ++ A + V WYK + + + ++ + ATL S DSG+Y A N G+ S
Sbjct: 8628 LSTKWFKDGKELTSDNKYKISFFNKVSGLKIINVVPGDSGVYSFEVQNPVGKDSCKVSIQVSDRIIPPSFTRKL--------KETNGL-----SGSSVVME--------------------CKVYGSP-------------PISVLWFHDGNEISSGRKYQTTLTDNTCALTVNMLEEADAGDYTCIATNVAGSDE---CSAPLTVRE-------------------------------------PPSFVQKPDPMDVLTGSNVTFTSIVKGSPPF-------------TVSWFKGSTELVPGARCNVSLQDSVGELELFDVDTSQSGEYTCIVSNEAGRASCTTRLFVKAPAIFVKRLNDYSIEKGKPLILEGTFSGTPPISVTWKKNGINVIASQRCNITTTEKSAILEILSSTVEDSGQYNCYIENASGKDSCSAQILILEPPYFVKQLEPV-KVTVGDSASLQCQL--AGTPEIGVSWYKGDTKLRPTATCKMHFKNNVATLVFTQVDSSDSGEYICRAENSVGEVSS 9057 HSP 9 Score: 67.3958 bits (163), Expect = 1.002e-10 Identity = 98/497 (19.72%), Postives = 163/497 (32.80%), Query Frame = 3
Query: 285 SELV-VDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYH-NGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINI 1769
+EL+ V G+P L V + L+ W+KDG+ L A ++ F L D+G Y + G++ T L ++ +N ++ D A ++P +S WFKDG L R + + G +L+I+ D G+++C A NS+G ++ ++ PPSF+ P V G+ K L+ W+ + E+V S K E + S+L + SD+G+Y C + + G VE D + + +FI++LEP L +GD L KV + + W+ N+ + S+ ++S+ TA L + EDSG+Y A N+ G I I
Sbjct: 5036 AELIQVTAGDPATLEYTVSGTPE--LKPKWYKDGRPLVASKKYRISFKNNIAQLKFYSAELHDSGQYTFEISNEVGSSSCETT------------FTVLDRDIAPLFTKPLRNVDSVVGGACRLDCKIAGSLPMRVS-------------------------------WFKDGKELTASDRYQIAFVEGTASLEISRVDMNDAGNFTCRATNSVGSKDSSGA----------------------------------------LIVQEPPSFV--TKPGSRDVLPGSAVCLKSAFQ--GSAPLTIKWFKGDKELVSGGSCYITK-ETSESSLELYAVKTSDSGTYTCKVSNVAGSVECS---------------------------------------ADLFVKEPATFIEKLEPSQLLKKGDGTQLACKV--TGTPPIKITWFANDRELRESSKHKMSFAESTAVLRLTDVAIEDSGEYMCEAQNEAGSDHCTGIVI 5401 HSP 10 Score: 63.929 bits (154), Expect = 1.004e-9 Identity = 146/714 (20.45%), Postives = 230/714 (32.21%), Query Frame = 3
Query: 291 LVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHH--SDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREI---------SVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVE-------SEPVTL-------NVQTEKSIILD----------------------------------------PQSKGISAIHALEAELNAPKAEMKEDEIITDL--PSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECL-PNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLTV-----IPMVDFRP 2213
+ V GE L +V A L + W+KDGKLL++ + F L IL ++D G Y Q NN+ K+ T D P F +L ++ G L LE S + WF + + G++ + C L + D G Y+CVA N G E C +E PPSF++ +P E+ SV G P FKV W+ + +R E ++ L + S +G Y CV+ + G+ EP T +V+ KSIIL+ +KG + ++ E E A + D +++ L P F+ +LEP++ + GDSV+L +V A + V W+K + + + + + ATL DSG+Y +A N G S+ I F+ +QE L P+F + +I G P +++G + W D VL+ D + ++ L+I + + +A+NPLG A A LT P D +P
Sbjct: 6726 MTVTVGETCALECKV--AGTPELSVEWYKDGKLLTSSQKHKFSFYNKISSLKILSVEKEDAGTYT-----------------------------------FQVQNNVGKSSCTAVV-----DVSDRMVPPSFTRRLKDT-----GGVLGTSCILECKVAGSSPISIAWFHEKTKIVSGAKYQTTFSDNVCTLQLNSLDSSDMGSYTCVAANVAGSDE---CRALLTVQE-------------------------------------PPSFVKEPEPLEVLPGKNITFTSVIRGTPP-FKV------------GWFRGARELVKGNRCNIYFEDTVAELELFNIDISQSGEYTCVVSNNAGQASCTTRLFVKEPATFVKKLSDHSVEPGKSIILEGTYTGTLPISVTWKKDGVSITPSERCNIVTTEKTCILEILSSTKGDAGHYSCEIENEAGRDAC--DALVSTLEPPYFVTELEPLEA-SVGDSVSLQCQV--AGTPEITVSWFKGDTKLRSTPEYRTYFTNNVATLVFNKVSINDSGEYTCMAENSIGTAASKTI----------------------------------------FR----IQERQLPPSFARQLKDIEQTVGLPVTLTCRLNGSAPIQVCWYRDGVLLRDDENLQMSFVDNVATLKILQTDLSHSGQYSCSASNPLGTASSTARLTAREPKKSPFFDIKP 7290 HSP 11 Score: 61.6178 bits (148), Expect = 6.165e-9 Identity = 96/466 (20.60%), Postives = 160/466 (34.33%), Query Frame = 3
Query: 363 INWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNS--LYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQS 1754
++WFK L G+R + L++ +G+Y C+ + +G A T+L K F+ +L N ++ +G+PL +E S+ W K+G+++ R ++ L+I ED+G Y+C N+ GK SC+ +Q+ LE PP F++ ++P +++V + A ++ P ++ WY ++ + + N++ L SD+G YIC + G V S L VQ +K PSF +QL + + +V L V E A S + V WYK+ + + +Q S+ ATLNI SG Y ATN G S
Sbjct: 8819 VSWFKGSTELVPGARCNVSLQDSVGELELFDVDTSQSGEYTCIVSNEAGRASCT---------------TRL----------------------------FVKAPAIFVKRL-NDYSIEKGKPLILEGTFSGTPPISVT--WKKNGINVIASQRCNITTTEKSAILEILSSTVEDSGQYNCYIENASGK---DSCS--------------------AQILILE-----------------PPYFVKQLEPVKVTVGDSASLQCQLAGTP----EIGVSWYKGDTKLRPTATCKMHFKNNVATLVFTQVDSSDSGEYICRAENSVGEVSSS-TFLTVQEQKL-----------------------------------PPSFSRQLRDVQE-----TVGLPVVFECAVSGSEPISVSWYKDGKPLKDSPNIQTSFLDNIATLNIFKTDRSLSGQYSCTATNPIGSASS 9153 HSP 12 Score: 60.4622 bits (145), Expect = 1.263e-8 Identity = 63/281 (22.42%), Postives = 119/281 (42.35%), Query Frame = 3
Query: 108 YNNNSNFNSVPDEGVIPNGTTMAPHALEVVNQVNKRSLASPR--RGSDHLYDSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYE---TGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDR--GYCALDIAFCYPEDNGDYSCVARNSLGK 929
Y + + + GV +G+T+ EV+ + + R ++ PT L++ V+ EGE + L + + S ++ W+++ + + F F G L I +D+G + C A + +GT ++ ++ + D T L E+ E ++ + T T E+ P F+S+ P L++G + E + + + W K G+ L G R V Y++ G C L I+ + +D G+Y+ V RN G+
Sbjct: 893 YKSQAGIEVKKEVGVSISGSTVREEHFEVLRGREAKVTETARVPAPAEVPVTPPTLVSGLKNVTVI-EGESVTLECHI--SGYPSPKVTWYREDYQIESSIDFQITFQGGIARLMIREAFAEDSGRFTCSAVNEAGTVSTS-CYLAVQVSEEFDKETTLTEKFATEEKRFVESRDVVMTDTSITEEQAGPGEPAAPFFISK-PVVQKLVEGGSVVFECQIGGNPKPHVY--WKKSGVPLTTGYRYKVSYNKQTGECRLVISMTFADDAGEYTIVIRNKHGE 1166 HSP 13 Score: 57.7658 bits (138), Expect = 8.869e-8 Identity = 78/331 (23.56%), Postives = 136/331 (41.09%), Query Frame = 3
Query: 978 ITKSNLTESSISQLKSLENPGEEYARYNDTSVIPM-PPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITD---------LPSFIKQLEPIDQ-LNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAI--SEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRL-KDLEEDLASGNISTFQNTEDVQEECLPNF 1928
I+ S + E L+ E E AR + +P+ PP+ + + + ++V EG + I K++ WY +++ + I+ L I D+G + C +E G V S L VQ + D ++ E + + D IT+ P FI + P+ Q L EG SV ++ + +V W K+ + G R ++SY++ T +V ++ ++D+G+Y ++ NK+G+T +AS+ L + A Y+ L K +E L +STF V E P F
Sbjct: 909 ISGSTVREEHFEVLRGREAKVTETARVPAPAEVPVTPPTLVSGL--KNVTVIEGESVTLECHISGYPSPKVT--WYREDYQIESSIDFQITFQGGIARLMIREAFAEDSGRFTCSAVNEAGTV-STSCYLAVQVSEE--FDKETTLTEKFATEEKRFVESRDVVMTDTSITEEQAGPGEPAAPFFISK--PVVQKLVEGGSVVFECQI--GGNPKPHVYWKKSGVPLTTGYRYKVSYNKQTGECRLVISMTFADDAGEYTIVIRNKHGET----------------SASASLLEEADYEALVKTQQEMLYQTQMSTFIQEPKVG-EIAPGF 1211 HSP 14 Score: 56.9954 bits (136), Expect = 1.318e-7 Identity = 107/498 (21.49%), Postives = 161/498 (32.33%), Query Frame = 3
Query: 279 LESELVVDEGEPLRLMTRVGPADDGSLE----------INWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNG 1742
LE V + EP+++ D SL+ ++W+K L + F L D+G+Y+C A++ G S+ T+ ++E K P F QL + + G P+ E + S+ W+KDG LK I + L+I +G YSC A N +G A + + K LTE PP F P+ P + V E A VT Q + W + +++ N + L I+ D+G Y C +E G+ + A+LN +KE I PSF K+L + EG+S L +V A + V WYKN I S +I + L + A D+G Y ATN G
Sbjct: 8971 LEPPYFVKQLEPVKVTV----GDSASLQCQLAGTPEIGVSWYKGDTKLRPTATCKMHFKNNVATLVFTQVDSSDSGEYICRAENSVGEVSSS------------------------------------TFLTVQEQKLP----PSFSRQLRDVQETV-GLPVVFECAVSGSEPISV--SWYKDGKPLKDSPNIQTSFLDNIATLNIFKTDRSLSGQYSCTATNPIGSASSGA-------------KLILTEGK------------------------NPPFFDIPLAPMDAVVGESADLECHVT----GTQPIKVTWAKDNREIRSGGNYQISYLENSAHLTIVKVDKGDSGQYTCYAVNEVGK----------------------------DSCTAQLN-----IKERLI---PPSFTKKLSETVEETEGNSFKLEGRV--AGSQPITVAWYKNNVEIHPTSNCEIMFKNNALLLQVKRASMADAGLYTCKATNDAG 9342 HSP 15 Score: 56.9954 bits (136), Expect = 1.364e-7 Identity = 57/223 (25.56%), Postives = 95/223 (42.60%), Query Frame = 3
Query: 360 EINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLH----IEIFLEH--HSDSSLVTEWFKDGMSLKLGSRIDVIYD-RGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESS 1007
+I WF +G LL+ + + +FD L IL+T D G+Y C+A + G A + AH+RI+P E E+ E+G +E+ K P F +L +P+H I E+ H + + WFK+ M L +I+ G + D+G Y C A+N G++ T + + PE++ + ++ E S
Sbjct: 3664 KIQWFFNGMLLTPSADYKFVFDGDTHSLIILFTRFQDEGEYTCLASNEYGKAVCS-AHLRISPRG----------ERSTEM-------ESGEKKALEKPKGPCP--PYFFKEL---------KPVHCGPGIPAVFEYSVHGEPAPTVLWFKEDMPLYTSVCYTIIHSPDGSGTFIVNDPQRGDSGLYLCKAQNLWGES-TCAAELLVLPEDTDVPDASCKEES 3856 HSP 16 Score: 56.6102 bits (135), Expect = 1.993e-7 Identity = 64/258 (24.81%), Postives = 107/258 (41.47%), Query Frame = 3
Query: 258 SPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSA----GSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQL 1019
+P F Q L+S +VV EG V + E++WF+DG+++S G + S F G L I + ++G Y A + SG A S E++ A+T P SQ S+T+ QG + +++ + + V ++++DG ++ + + +L IA YPED+G YS A NS+G+A T + + EE + K T S +Q+
Sbjct: 5 APMFTQPLQS-VVVLEGSTATFEAHV--SGSPVPEVSWFRDGQVISTSTLPGVQIS--FSDGRARLMIPAVTKANSGRYSLRATNGSGQATSTA-------------------ELLV----------------------TAETAPPNFSQRLQSMTVRQGSQVRLQVRV--TGIPTPVVKFYRDGAEIQSSLDFQISQEGDLYSLLIAEAYPEDSGTYSVNATNSVGRA-TSTAELVVQGEEVVPAKKTKTIVSTAQI 213 HSP 17 Score: 55.0694 bits (131), Expect = 5.673e-7 Identity = 48/187 (25.67%), Postives = 81/187 (43.32%), Query Frame = 3
Query: 1476 EIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEG----EKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEWR 2024
E+ P+ + L+ + + EG+SVTL + + S V WY+ + I + QI++ G A L I A +EDSG + A N+ G ++ SC+ E+ ++L A ++ D+ + S + E P F KP+ EGG F ++ G P P + W+
Sbjct: 940 EVPVTPPTLVSGLKNVTVI-EGESVTLECHI--SGYPSPKVTWYREDYQIESSIDFQITFQGGIARLMIREAFAEDSGRFTCSAVNEAGT-----VSTSCYLAVQVSEEFDKETTLTEKFATEEKRFVESRDVVMTDTSITEEQAGPGEPAAPFFISKPVVQKLVEGGSVVFECQIGGNPKPHVYWK 1118 HSP 18 Score: 55.0694 bits (131), Expect = 6.278e-7 Identity = 98/497 (19.72%), Postives = 165/497 (33.20%), Query Frame = 3
Query: 249 LYDSPTFQQDLESELVVDEGEPLRLMTRVGPADDGS--LEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATN 1733
+ + P F + LE + GEP+ L +V DG+ + I+W+K+ L + + F L I D G+Y C A++ G S+ V +LP +++ ++ + G P+ E + + L W+KDG LK + + + + L I G Y+C A N LG A + + K L+E + PP F + P + ++ G FK + K++ W + ++ L N + L +L D G Y C + G+ +S L VQ + P FIK+L+ + + + K+ + V WYK+E I S+ ++S++ A L + S EDSGDY A N
Sbjct: 8029 VLEPPYFIEPLE-HVEAAIGEPITLQCKV----DGTPEIRISWYKEHTKLRSAPAYKMQFKNNVASLVINKVDHSDVGEYTCKAENSVGAVASSAVLVIKE--------RKLPPSFARKLKDVHETL---------------------------------GFPVAFECRI--NGSEPLQVSWYKDGELLKDDANLQMSFVHHVATLQILQTDQSHVGQYNCSASNPLGTASSSA-------------KLILSEHEV------------------------PPFF--DLKPVSVDLALGESGSFKCHVTGTAPIKIT--WAKDNREIRPGGNYKMTLVENTATLTVLKVAKGDAGQYTCYASNVAGK-DSCSAQLGVQ--------------------------------------EPPRFIKKLDQSRIVKQDEYTRYECKI--GGSPEIKVLWYKDEVEIQESSKFRMSFEDSVAILEMHSLSVEDSGDYTCEARN 8395 HSP 19 Score: 53.5286 bits (127), Expect = 1.601e-6 Identity = 28/90 (31.11%), Postives = 47/90 (52.22%), Query Frame = 3
Query: 1479 IITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQT 1748
++T+ P F+K+LE + GDS L K+ + V WY+NE+ + + Q+++ A + + S +EDSGD+ A N G T
Sbjct: 6431 LVTEPPKFVKKLEASKIIKAGDSARLECKI--TGSPEIQVVWYRNEHELTASDKYQMTFIDSVAVIQMNSLGTEDSGDFICEAQNPAGST 6518
BLAST of Titin vs. Ensembl Mouse
Match: Ttn (titin [Source:MGI Symbol;Acc:MGI:98864]) HSP 1 Score: 109.383 bits (272), Expect = 1.516e-23 Identity = 177/772 (22.93%), Postives = 302/772 (39.12%), Query Frame = 3
Query: 360 EINWFKDGKLLSAGSRFSTIF-DRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPE-EMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLS---------------------QL-----------PNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSL--KLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIV--KTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPI------DQLNEGD-----------------SVTLT--------VKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLAD-SAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLTVIPMVDFRPDLRHI---TPE----NPFKKMAGLKKVEC---TSELNDAFKKSKPTAERIVKIEMGSET---KSKFYKSPDVIETEKLYDQVAA 2426
+I W+KDGK + G R+ F G L I + +D G Y A + G A + + + P + T +P E + I ++ + + M A+ P +S QL P S ++GQ ++ + T WF +G + ++ VI + G +L I P D+G+++ VA+N GK S I+ + E+ Q+K P+F+E + + +++ EG+ KV + W N +I+ R+ + +AL I + D+ Y ++ GR ++ +NV+ E +P+ E +L P+ + EI +LEP+ +Q EGD S+ L ++ P D ++ VEW + + +R+++ + G +L+ +A S DSG ATNK G + I + EK + S L D G QR+++LE G ++ T D +E+ P+ + P + EG A+F +V+G+P P + W ++ L+ + DG I+YL+I C +V V A NP GV + + L + DFR LR PE P K ++KV+ TSE + K + AERI ++ E+ +SKF + + E Y+ + A
Sbjct: 1323 KIAWYKDGKRIRHGERYQMDFLQDGRASLRIPVVLPEDEGIYTAFASNIKGNAICS-GKLYVEPAAPFSAPTYMPTPEAVSRIRSVSPRSLSRSPIRMSPAMSPARMSPARMSPARMSPARMSPGRRLEETDESQLERLYKPVFVLKPASFKCLEGQTARFDLKVVGRPMPE--TFWFHNGQQIVNDYTHKV-VIKEDGTQSLIIVPASPSDSGEWTVVAQNRAGK--------------STISVTLTVEAVEHQIK---------------------PAFVEKL--KNVNIKEGSRLEMKVRATGNPNPDIV--WLKNSDIIVPHKYPRIRIEGTRGEAALKIDSIISQDSAWYTATAINKAGR-DTTRCKVNVEVE---FAEPEP---------ERKLIIPRGTYRAKEIAAP------ELEPLHLRYGQEQWEEGDLYDKEKQQKPFFKKKLTSLRLKRFGPAHFECRLTPIGDPTMVVEWLHDGKPLEAANRLRMINEFGYCSLDYGAAYSRDSGVITCRATNKYGTDHTSATLI--VKDEKSLVEESQLPDGKKGLQRIEELERMAHEGALTGV--TTDQKEKQKPDIVLFPEPVRVLEGETARFRCRVTGYPQPKVNWYLNGQLIRKSKRFRVRYDG-IHYLDIVDCKSYDTGEVKVTAENPEGVTEHKVKLEIQQREDFRSVLRRAPEPKPEFHVHEPGKLQFEVQKVDRPVDTSETKEVVKLKR--AERITHEKVSEESEELRSKFKRRTE----EGYYEAITA 2021 HSP 2 Score: 83.1889 bits (204), Expect = 1.351e-15 Identity = 135/670 (20.15%), Postives = 238/670 (35.52%), Query Frame = 3
Query: 357 LEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTA-----------------------QSNIAHVRINPES--------GVDYLTQLPEE----MMQEINNL----DKNYETGTYHGMEEDKHAAKTI-------PKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLTVIPMVDFRPDLRHI 2228
+ + W KDG+ L R ++ L I T D G Y VA + TA Q + +N + G++ Q+ E + + I+ + ++ + G Y + ++ T+ P+ L +L +T+ G+P + + W+K+ L G + ++D L + +PED Y+C A+N G A T + PE + +P +E Y PP+ + P+ ++ SEG PA F+ + +G L WY + +K + L I P D G+Y V + G+V S TL ++ +SI+ + + I E E+ A P +++EP+ ++ G T +++ A + + +W+K I + I ++L I+ D G+Y A+N+ G ++SC T V E P F +P +T G AKFL VSG P + W+ D + P C+ + + + LE++ + A+N G +A LT+I F +L +
Sbjct: 3092 ITVQWMKDGQELQIADRIKIQKEKYVHRLLIPSTRMSDAGKYTVVAGGNMSTANLFVEGRDVRIRSIKKEVQVIEKQRAVVEFEVNEDDVDAHWYKDGIEINFQVQERHQYVVERRIHRMFISETRHSDAGEYTFVAGRNRSSVTLYVNAPEPPQVLQEL-QPVTVQSGKPARFCAVISGRPQPKI--SWYKEEQLLSTGFKCKFLHDGQEYTLLLIEAFPEDAAVYTCEAKNDYGVATTSASLSVEVPE-------------------VVSPDQEMPVY--------PPAIVTPL--QDTVTSEGRPARFQCQV---SGTDLKVSWYCKDKKIKPSRFFRMTQFEDTYQLEIAEAYPEDEGTYAFVANNAVGQVSST-ATLRLEAPESILHERIGQQI------EMEMKA-------------APVIKRRIEPL-EVALGHLAKFTCEIQGAPN--VRFQWFKAGREIYESDKCSIRSSNYVSSLEILRTQVVDCGEYTCKASNEYG-------SVSC-------------------------------------TATLTVTEAYPPTFLSRPKALTTFVGKAAKFLCTVSGTPVIEIIWQKDGAALSPSPDCRVTDADNKHSLELSNLTVQDRGIYSCKASNKFGADICQAELTIIDKPHFIKELEAV 3659 HSP 3 Score: 76.2554 bits (186), Expect = 1.775e-13 Identity = 78/355 (21.97%), Postives = 130/355 (36.62%), Query Frame = 3
Query: 678 PKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNG 1742
P + + P +T+ G P +E + + L +WFKDG L GSR + + R +L I D G Y+ N +GK+ SC V+ H + ++ PPSF+ + ++ S + GA + + +S W+ +G + + + N+ L + PSDTG+Y CV + G+ ES V L VQ + PSF + + ++ L G S+T T + V+W+K +++G IS + L ++ SGDY + TN G
Sbjct: 6638 PATIVEKPEPMTVTTGNPFTLECVVAGTPE--LSAKWFKDGRELSSGSRHHITFVRNLASLKIPSAEMNDKGLYTFEVENRVGKS---SCTVSVHVSDRVV----------------------------------PPSFVRRL--KDTSATLGASVVLECRV--SGSAPISVGWFLDGNEIISSPKCQSSFADNVCTLTLSSLEPSDTGAYTCVAANVAGQDESSAV-LTVQ--------------------------------------EPPSFEQTPDSVEVL-PGMSLTFTSVIR--GTPPFKVKWFKGSRELVSGEACTISLEDFVTELELLEVEPGQSGDYSCLVTNDAG 6907 HSP 4 Score: 71.2478 bits (173), Expect = 6.603e-12 Identity = 118/561 (21.03%), Postives = 202/561 (36.01%), Query Frame = 3
Query: 180 HALEVVN-QVNKRSL----ASPRRGSD------HLYDSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAG 1829
H+LE+ N V R + AS + G+D + D P F ++LE+ V ++ +D + I W KDG+ L AG + F+ L+I D+G Y C A + +G++ S+ A P P F+ ++ S ++ G+ + L+ + WFK+ L + + + + LDI +D+G YSC A N +G + SC+ VI PPSFI+ ++P +I GA A + I G G +W+ + + +++ + + L I +D G Y CV+ +E G+ G A H L+ + P+F+K+++ L G +VTL V + V W K + I ++++S+ G A L I G Y +A N+ G +Q+ + E KI+ + L+ +AG
Sbjct: 3612 HSLELSNLTVQDRGIYSCKASNKFGADICQAELTIIDKPHFIKELEA---VQSAINKKIHLECQVDEDRKVTITWSKDGQKLPAGKDYKIYFEDKIASLEIPLAKLKDSGTYTCTASNEAGSSSSSAAVAVREP-------------------------------------------PSFVKKVDPSYLMLPGESARLHCKLK--GSPVIQVTWFKNNKELSESNTVRMSFVNSEAILDITDVKVDDSGTYSCEATNDVG---SDSCSTEV-------------------------------------VIKEPPSFIKTLEPADI--VRGANALLQCEIA-GTGP-FEVNWFKDKKQIRSSKKYRLFTQKTFVYLEISSFNSADVGDYECVVANEVGKC----------------------GCVATHLLK-----------------EPPTFVKKVDDFTAL-AGQTVTLQAAVR--GSEPISVMWMKGQEVIKEDGKIKMSFSNGVAVLTIPDVQISLGGKYTCLAENEAG-SQTSVGELIVKEPAKIIERAELIQVTAG 4037 HSP 5 Score: 71.2478 bits (173), Expect = 7.432e-12 Identity = 101/514 (19.65%), Postives = 178/514 (34.63%), Query Frame = 3
Query: 357 LEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPE---------------------------EMMQEINNLDKNYE---------------TGTY-------HGMEEDKHAAKTI-PKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVE-SEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQ 1745
+++W KD + L +G ++ + + + IL D G+Y C A + G+ + P V LT + E+++E +N+ +Y G Y GM+E + P + + P S+ + G +E + + L T+WFKDG L ++ + + L I P D+G YS +N +GK SC V+ + II PPSF + +E + G+ + + +S W+H+G + + + L N AL + +D G Y C+ + G E S P+T + + PSF+++ +P+D L G +VT T V+ V W+K ++ G+R +S L + + SG+Y I +N+ G+
Sbjct: 7422 FQVSWHKDKRELRSGKKYKIMSENLLTSIHILNVDTADIGEYQCKATNDVGSDTCVGSVTMKAPPQFVKKLTDISTIIGKEVQLQTTIEGAEPISVAWFKDKGEIVRESDNIWISYSENIATLQFSRAEPANAGKYTCQIKNDAGMQECYATLSVLEPAAIVEKPESIKVTTGDTCTLECTVSGTPE--LSTKWFKDGKELTSDNKYKISFFNKVSGLKIINVVPGDSGVYSFEVQNPVGK---DSCKVSIQVSDRII----------------------------------PPSFTRKL--KETNGLSGSSVVMECKV--YGSPPISVLWFHDGNEISSGRKYQTTLTDNTCALTVNMLEEADAGDYTCIATNVAGSDECSAPLT----------------------------------------VREPPSFVQKPDPMDVLT-GSNVTFTSIVK--GSPPFTVSWFKGSTELVPGARCNVSLQDSVGELELFDVDTSQSGEYTCIVSNEAGR 7849 HSP 6 Score: 67.781 bits (164), Expect = 7.645e-11 Identity = 108/532 (20.30%), Postives = 176/532 (33.08%), Query Frame = 3
Query: 357 LEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIA---HVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREI---------SVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRV------------------------ESEPVTLN----------------------------VQTEKSIILDPQSKGI--SAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQS 1754
L WFKDGK L++ +++ F L I+ + D+G Y ++ G ++ RI P S L +E N L +G+ ME + G P + WF DG + G + CAL + D GDY+C+A N G E C+ E PPSF++ DP ++ S+ +G+P + + W+ + +R L+ ++ L + S +G Y C++ +E GR + +P+ L TEKS IL+ S + S + E + K +I + P F+KQLEP+ ++ GDS +L ++ A + V WYK + + + ++ + ATL S DSG+Y A N G+ S
Sbjct: 7609 LSTKWFKDGKELTSDNKYKISFFNKVSGLKIINVVPGDSGVYSFEVQNPVGKDSCKVSIQVSDRIIPPSFTRKL--------KETNGL-----SGSSVVME--------------------CKVYGSP-------------PISVLWFHDGNEISSGRKYQTTLTDNTCALTVNMLEEADAGDYTCIATNVAGSDE---CSAPLTVRE-------------------------------------PPSFVQKPDPMDVLTGSNVTFTSIVKGSPPF-------------TVSWFKGSTELVPGARCNVSLQDSVGELELFDVDTSQSGEYTCIVSNEAGRASCTTRLFVKAPAIFVKRLNDYSIEKGKPLILEGTFSGTPPISVTWKKNGINVIASQRCNITTTEKSAILEILSSTVEDSGQYNCYIENASGKDSCSAQILILEPPYFVKQLEPV-KVTVGDSASLQCQL--AGTPEIGVSWYKGDTKLRPTATCKMHFKNNVATLVFTQVDSSDSGEYICRAENSVGEVSS 8038 HSP 7 Score: 67.781 bits (164), Expect = 7.841e-11 Identity = 115/569 (20.21%), Postives = 192/569 (33.74%), Query Frame = 3
Query: 42 RHKYSFIFNLDFIAMTTQESMEYNNNSNFNSVPDEGVIPNGTTMAPHALEVVNQVNKRSLASPRRGSDHLY--DSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNG 1742
+HK+SF N F+ ++ E GT + N+ A + S HL + P F + +S+ V + G ++L VG + I WFKD K L G+ S D +L++ D+G Y+C + GT S P P+F+ + L L GQ E + + + W+ DG + + + + G I+ E++G Y C ARN G T SC++ +LK E PP FI ++P E+ +V W N + +++ + T +M++ L+I PSD G Y C++ +E G S SA AL+ + PSFIK++E + + + + T + A + + W K++ + + IS++ ATL + S + SG Y A N++G
Sbjct: 4726 KHKFSFHDNTAFLEISQLE---------------------GTDSGTYTCSATNK------AGHSQCSGHLTVKEPPYFVEKPQSQDV-NPGTRVQLKALVG--GTAPMTIKWFKDNKELHPGAARSVWKDDTSTILELFSAKAADSGTYICQLSNDVGTTSSKATIFVKEP-------------------------------------------PQFIKKPSPVLVLRNGQSTTFECQVTGTPE--IRVSWYLDGNEITDLRKYGISFVDGLATFQISNARVENSGTYVCEARNDAG---TASCSI--------------------ELKVKE-----------------PPIFIRELEPVEVVKDSDVELECEVM----GTTPFEVTWLKNNKEIRSGKKYTMSEKMSVFYLHITKCDPSDVGEYQCIIANEGG----------------------SCACSARVALK-----------------EPPSFIKKIENVTTVLKSSA---TFQSTVAGSPPISITWLKDDQILEENDNVHISFEDSVATLQVRSVDNGHSGRYTCQAKNESG 5133 HSP 8 Score: 67.0106 bits (162), Expect = 1.269e-10 Identity = 98/497 (19.72%), Postives = 163/497 (32.80%), Query Frame = 3
Query: 285 SELV-VDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYH-NGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINI 1769
+EL+ V G+P L V + L+ W+KDG+ L A ++ F L D+G Y + G++ T L ++ +N ++ D A ++P +S WFKDG L R + + G +L+I+ D G+++C A NS+G ++ ++ PPSF+ P V G+ K L+ W+ + E+V S K E + S+L + SD+G+Y C + + G VE D + + +FI++LEP L +GD L KV + + W+ N+ + S+ ++S+ TA L + EDSG+Y A N+ G I I
Sbjct: 4029 AELIQVTAGDPATLEYTVSGTPE--LKPKWYKDGRPLVASKKYRISFKNNIAQLKFYSAELHDSGQYTFEISNEVGSSSCETT------------FTVLDRDIAPLFTKPLRNVDSVVGGACRLDCKIAGSLPMRVS-------------------------------WFKDGKELTASDRYQIAFVEGTASLEISRVDMNDAGNFTCRATNSVGSKDSSGA----------------------------------------LIVQEPPSFV--TKPGSRDVLPGSAVCLKSAFQ--GSAPLTIKWFKGDKELVSGGSCYITK-ETSESSLELYAVKTSDSGTYTCKVSNVAGSVECS---------------------------------------ADLFVKEPATFIEKLEPSQLLKKGDGTQLACKV--TGTPPIKITWFANDRELRESSKHKMSFAESTAVLRLTDVAIEDSGEYMCEAQNEAGSDHCTGIVI 4394 HSP 9 Score: 66.6254 bits (161), Expect = 1.887e-10 Identity = 120/599 (20.03%), Postives = 216/599 (36.06%), Query Frame = 3
Query: 354 SLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQ-----LPEEMMQEINNLD----------------------KNYETGTYHGMEEDKHAAK-----------------TIPKFLSQL-------PNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPI--DPREISVSEGAPA----YFKVTIDPGNGQKLSND-----------------------WYHNGEIVKTCSRLTGKLEMNISA-----LNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSI--ILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVT-LTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSE-KINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNIST 1883
+ E+ W+ +G L S+ G + L+IL +D G Y V ++ G A S+ A + + + Y +Q +P+ + E+ + K+ T T + +H A T+ K + L P S+T+ +G+ + + W ++G + +R V + +I+ D G+YS V NS GK E Q + K+ + E +++ +++P E + +T P EP+ P+ +S +E P + V+ P Q L + WY +G+ +K G + + SA L I SD G Y+C + E G ++ Q+ KSI + S+ ++ A K E E I+ P + L D DSV T+KV + + W K+ I GS+ ++S ++ L I+ + D G Y TN G S K+ I + + S+ Q+ +E+++ +++
Sbjct: 32445 TAEVKWYHNGVELQESSKIHYTNTSGVLTLEILDCQTEDGGTYRAVCTNYKGEA-SDYATLDVTGGAYTTYASQRRDEEVPKSVFPELTKTEAYAVSSFKRTSELEAASSVREVKSQMTETRESLSTYEHYASAEMKSATSEEKSLEEKATVRKIKTTLAARILTKPRSITVHEGESARFS--CDTDGEPVPTVTWLREGQVVSTSARHQVTTTKYKSTFEISSVQASDEGNYSVVVENSDGKQEAQFT--------LTVQKARVIEKAVTSPPRVKSP-EPRVKSPETVKSPKRVKSPEPVTSHPKAVSPTETKPTEKGQHLPVSAPPKITQSLKAEASKDIAKLTCAVESSALCAKEVAWYKDGKKLKE----NGHFQFHYSADGTYELKIHNLSESDCGEYVCEVSGEGGTSKTS-FQFTGQSFKSIHEQVSSISETTKSVQKTAESAEAKKQEPIAPESISSKPVIVTGLR--DTTVSSDSVAKFTIKV--TGEPQPTITWTKDGKAIAQGSKYKLSSNKEEFILEILKTETSDGGLYACTVTNSAGSVSSSCKLTIKAVKDTEAQKVSTQKTSEVTSQKKASAQEEISQKALTS 33022 HSP 10 Score: 63.929 bits (154), Expect = 9.954e-10 Identity = 146/714 (20.45%), Postives = 230/714 (32.21%), Query Frame = 3
Query: 291 LVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHH--SDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREI---------SVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVE-------SEPVTL-------NVQTEKSIILD----------------------------------------PQSKGISAIHALEAELNAPKAEMKEDEIITDL--PSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECL-PNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLTV-----IPMVDFRP 2213
+ V GE L +V A L + W+KDGKLL++ + F L IL ++D G Y Q NN+ K+ T D P F +L ++ G L LE S + WF + + G++ + C L + D G Y+CVA N G E C +E PPSF++ +P E+ SV G P FKV W+ + +R E ++ L + S +G Y CV+ + G+ EP T +V+ KSIIL+ +KG + ++ E E A + D +++ L P F+ +LEP++ + GDSV+L +V A + V W+K + + + + + ATL DSG+Y +A N G S+ I F+ +QE L P+F + +I G P +++G + W D VL+ D + ++ L+I + + +A+NPLG A A LT P D +P
Sbjct: 5707 MTVTVGETCALECKV--AGTPELSVEWYKDGKLLTSSQKHKFSFYNKISSLKILSVEKEDAGTYT-----------------------------------FQVQNNVGKSSCTAVV-----DVSDRMVPPSFTRRLKDT-----GGVLGTSCILECKVAGSSPISIAWFHEKTKIVSGAKYQTTFSDNVCTLQLNSLDSSDMGSYTCVAANVAGSDE---CRALLTVQE-------------------------------------PPSFVKEPEPLEVLPGKNITFTSVIRGTPP-FKV------------GWFRGARELVKGNRCNIYFEDTVAELELFNIDISQSGEYTCVVSNNAGQASCTTRLFVKEPATFVKKLSDHSVEPGKSIILEGTYTGTLPISVTWKKDGVSITPSERCNIVTTEKTCILEILSSTKGDAGHYSCEIENEAGRDAC--DALVSTLEPPYFVTELEPLEA-SVGDSVSLQCQV--AGTPEITVSWFKGDTKLRSTPEYRTYFTNNVATLVFNKVSINDSGEYTCMAENSIGTAASKTI----------------------------------------FR----IQERQLPPSFARQLKDIEQTVGLPVTLTCRLNGSAPIQVCWYRDGVLLRDDENLQMSFVDNVATLKILQTDLSHSGQYSCSASNPLGTASSTARLTAREPKKSPFFDIKP 6271 HSP 11 Score: 61.2326 bits (147), Expect = 7.805e-9 Identity = 96/466 (20.60%), Postives = 160/466 (34.33%), Query Frame = 3
Query: 363 INWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNS--LYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQS 1754
++WFK L G+R + L++ +G+Y C+ + +G A T+L K F+ +L N ++ +G+PL +E S+ W K+G+++ R ++ L+I ED+G Y+C N+ GK SC+ +Q+ LE PP F++ ++P +++V + A ++ P ++ WY ++ + + N++ L SD+G YIC + G V S L VQ +K PSF +QL + + +V L V E A S + V WYK+ + + +Q S+ ATLNI SG Y ATN G S
Sbjct: 7800 VSWFKGSTELVPGARCNVSLQDSVGELELFDVDTSQSGEYTCIVSNEAGRASCT---------------TRL----------------------------FVKAPAIFVKRL-NDYSIEKGKPLILEGTFSGTPPISVT--WKKNGINVIASQRCNITTTEKSAILEILSSTVEDSGQYNCYIENASGK---DSCS--------------------AQILILE-----------------PPYFVKQLEPVKVTVGDSASLQCQLAGTP----EIGVSWYKGDTKLRPTATCKMHFKNNVATLVFTQVDSSDSGEYICRAENSVGEVSSS-TFLTVQEQKL-----------------------------------PPSFSRQLRDVQE-----TVGLPVVFECAVSGSEPISVSWYKDGKPLKDSPNIQTSFLDNIATLNIFKTDRSLSGQYSCTATNPIGSASS 8134 HSP 12 Score: 60.4622 bits (145), Expect = 1.457e-8 Identity = 63/281 (22.42%), Postives = 119/281 (42.35%), Query Frame = 3
Query: 108 YNNNSNFNSVPDEGVIPNGTTMAPHALEVVNQVNKRSLASPR--RGSDHLYDSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYE---TGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDR--GYCALDIAFCYPEDNGDYSCVARNSLGK 929
Y + + + GV +G+T+ EV+ + + R ++ PT L++ V+ EGE + L + + S ++ W+++ + + F F G L I +D+G + C A + +GT ++ ++ + D T L E+ E ++ + T T E+ P F+S+ P L++G + E + + + W K G+ L G R V Y++ G C L I+ + +D G+Y+ V RN G+
Sbjct: 893 YKSQAGIEVKKEVGVSISGSTVREEHFEVLRGREAKVTETARVPAPAEVPVTPPTLVSGLKNVTVI-EGESVTLECHI--SGYPSPKVTWYREDYQIESSIDFQITFQGGIARLMIREAFAEDSGRFTCSAVNEAGTVSTS-CYLAVQVSEEFDKETTLTEKFATEEKRFVESRDVVMTDTSITEEQAGPGEPAAPFFISK-PVVQKLVEGGSVVFECQIGGNPKPHVY--WKKSGVPLTTGYRYKVSYNKQTGECRLVISMTFADDAGEYTIVIRNKHGE 1166 HSP 13 Score: 57.7658 bits (138), Expect = 7.945e-8 Identity = 78/331 (23.56%), Postives = 136/331 (41.09%), Query Frame = 3
Query: 978 ITKSNLTESSISQLKSLENPGEEYARYNDTSVIPM-PPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITD---------LPSFIKQLEPIDQ-LNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAIS--EDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRL-KDLEEDLASGNISTFQNTEDVQEECLPNF 1928
I+ S + E L+ E E AR + +P+ PP+ + + + ++V EG + I K++ WY +++ + I+ L I D+G + C +E G V S L VQ + D ++ E + + D IT+ P FI + P+ Q L EG SV ++ + +V W K+ + G R ++SY++ T +V +++ +D+G+Y ++ NK+G+T +AS+ L + A Y+ L K +E L +STF V E P F
Sbjct: 909 ISGSTVREEHFEVLRGREAKVTETARVPAPAEVPVTPPTLVSGL--KNVTVIEGESVTLECHISGYPSPKVT--WYREDYQIESSIDFQITFQGGIARLMIREAFAEDSGRFTCSAVNEAGTV-STSCYLAVQVSEE--FDKETTLTEKFATEEKRFVESRDVVMTDTSITEEQAGPGEPAAPFFISK--PVVQKLVEGGSVVFECQI--GGNPKPHVYWKKSGVPLTTGYRYKVSYNKQTGECRLVISMTFADDAGEYTIVIRNKHGET----------------SASASLLEEADYEALVKTQQEMLYQTQMSTFIQEPKVG-EIAPGF 1211 HSP 14 Score: 57.3806 bits (137), Expect = 1.058e-7 Identity = 111/484 (22.93%), Postives = 187/484 (38.64%), Query Frame = 3
Query: 1110 PREISVSEGAPAYFKVTIDPGNGQKLSN-DWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQT----EKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLE--PIDQ-----LNEGDSVTLTVKVEPANDNSLY-------------VEWYKNENHIINGSRMQISYDR-GTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLP-NFDVKPM------NITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVHVA-ATNPLGVAKEEATLTVIPMVDFRPDLRHITPENPFKKMAGLKKVECTSE----LNDAFKKSKPTAERIVKIEMGS-ETKSKFYKSPDVIETEKLYDQVAAGLRKNI 2444
PR I+V EG A F D G+ + W G++V T +R S I SD G+Y V+ + G+ E++ TL VQ EK++ P+ K + +PK +K E +T P + E P ++ ++ +T ++K E + D + V WYK+ + Q Y GT L I + D G+Y + + G +++ F G+ + ++++ + E S + QE P + KP+ + T AKF +KV+G P PT+ W D + S K ++ LEI + +E D ++ TN G LT+ + D +K++ K E TS+ + + T+E I E+ S ET + ++ V+ E++ AA L K+I
Sbjct: 32610 PRSITVHEGESARFSCDTD---GEPVPTVTWLREGQVVSTSARHQVTTTKYKSTFEISSVQASDEGNYSVVVENSDGKQEAQ-FTLTVQKARVIEKAVTSPPRVKSPEPRVKSPETVKSPK-RVKSPEPVTSHPKAVSPTETKPTEKGQHLPVSAPPKITQSLKAEASKDIAKLTCAVESSALCAKEVAWYKDGKKLKENGHFQFHYSADGTYELKIHNLSESDCGEYVCEVSGEGGTSKTS----FQFTGQSFKSI---------HEQVSSISETTKSVQKTAESAEAKKQEPIAPESISSKPVIVTGLRDTTVSSDSVAKFTIKVTGEPQPTITWTKDGKAIAQGSKYKLSSNKEEFILEILK-TETSDGGLYACTVTNSAGSVSSSCKLTIKAVKD-----------TEAQKVSTQKTSEVTSQKKASAQEEISQKALTSEEIKMSEVKSHETLAIKEEASKVLIAEEVKRSAAASLEKSI 33063 HSP 15 Score: 56.6102 bits (135), Expect = 1.847e-7 Identity = 107/498 (21.49%), Postives = 161/498 (32.33%), Query Frame = 3
Query: 279 LESELVVDEGEPLRLMTRVGPADDGSLE----------INWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNG 1742
LE V + EP+++ D SL+ ++W+K L + F L D+G+Y+C A++ G S+ T+ ++E K P F QL + + G P+ E + S+ W+KDG LK I + L+I +G YSC A N +G A + + K LTE PP F P+ P + V E A VT Q + W + +++ N + L I+ D+G Y C +E G+ + A+LN +KE I PSF K+L + EG+S L +V A + V WYKN I S +I + L + A D+G Y ATN G
Sbjct: 7952 LEPPYFVKQLEPVKVTV----GDSASLQCQLAGTPEIGVSWYKGDTKLRPTATCKMHFKNNVATLVFTQVDSSDSGEYICRAENSVGEVSSS------------------------------------TFLTVQEQKLP----PSFSRQLRDVQETV-GLPVVFECAVSGSEPISV--SWYKDGKPLKDSPNIQTSFLDNIATLNIFKTDRSLSGQYSCTATNPIGSASSGA-------------KLILTEGK------------------------NPPFFDIPLAPMDAVVGESADLECHVT----GTQPIKVTWAKDNREIRSGGNYQISYLENSAHLTIVKVDKGDSGQYTCYAVNEVGK----------------------------DSCTAQLN-----IKERLI---PPSFTKKLSETVEETEGNSFKLEGRV--AGSQPITVAWYKNNVEIHPTSNCEIMFKNNALLLQVKRASMADAGLYTCKATNDAG 8323 HSP 16 Score: 56.6102 bits (135), Expect = 1.976e-7 Identity = 96/543 (17.68%), Postives = 180/543 (33.15%), Query Frame = 3
Query: 249 LYDSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSK---------------------------------GISAIHALEAE--------LNAPKAEMKEDEIITDL--PSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQT 1748
L + P+F + +E+ V + T G + I W KD ++L F+ L + +G Y C AK+ SG + L Q P +++++ ++D + + P+ +E + + L +W KDG + + ++ + I +D+G Y+ N G + SC+ Y R D + PPSF + + + + KV+ +S W+ +G+ + T ++ N +L + DT +Y C + + G + L V+ S ++ P+ + G LE +++ K ++ D+ P F+K+LE + GDS L K+ + V WY+NE+ + + Q+++ A + + S +EDSGD+ A N G T
Sbjct: 5050 LKEPPSFIKKIENVTTVLKSSATFQSTVAG---SPPISITWLKDDQILEENDNVHISFEDSVATLQVRSVDNGHSGRYTCQAKNESGIERCYAF-----------LLVQEPAQIIEKAKSVD---------------------------------VTEKDPVTLECVVAGTPE--LKVKWLKDGKQIVPSRYFSMSFENNVASFRIQSVMKQDSGQYTFKVENDFG---SSSCDA-------------------------------YLRVLDQDI---PPSFTKKLTKMDKVLGSSIHMECKVS----GSLPISAQWFKDGKEISTSAKYRLVCHENTVSLEVSNLELEDTANYTCKVSNVAGDNACSGI-LTVKEPPSFLVKPERQQAIPDSTVEFKAVLKGTPPFKIKWLKDDVELVSGPKCFIGLEGSTSFLNLYSVDSSKTGQYTCQVTNDVEPPKFVKKLEASKIIKAGDSARLECKI--TGSPEIQVVWYRNEHELTASDKYQMTFIDSVAVIQMNSLGTEDSGDFICEAQNPAGST 5499 HSP 17 Score: 56.225 bits (134), Expect = 2.419e-7 Identity = 64/258 (24.81%), Postives = 107/258 (41.47%), Query Frame = 3
Query: 258 SPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSA----GSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQL 1019
+P F Q L+S +VV EG V + E++WF+DG+++S G + S F G L I + ++G Y A + SG A S E++ A+T P SQ S+T+ QG + +++ + + V ++++DG ++ + + +L IA YPED+G YS A NS+G+A T + + EE + K T S +Q+
Sbjct: 5 APMFTQPLQS-VVVLEGSTATFEAHV--SGSPVPEVSWFRDGQVISTSTLPGVQIS--FSDGRARLMIPAVTKANSGRYSLRATNGSGQATST-------------------AELLV----------------------TAETAPPNFSQRLQSMTVRQGSQVRLQVRV--TGIPTPVVKFYRDGAEIQSSLDFQISQEGDLYSLLIAEAYPEDSGTYSVNATNSVGRA-TSTAELVVQGEEVVPAKKTKTIVSTAQI 213 HSP 18 Score: 55.0694 bits (131), Expect = 5.817e-7 Identity = 48/187 (25.67%), Postives = 81/187 (43.32%), Query Frame = 3
Query: 1476 EIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEG----EKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEWR 2024
E+ P+ + L+ + + EG+SVTL + + S V WY+ + I + QI++ G A L I A +EDSG + A N+ G ++ SC+ E+ ++L A ++ D+ + S + E P F KP+ EGG F ++ G P P + W+
Sbjct: 940 EVPVTPPTLVSGLKNVTVI-EGESVTLECHI--SGYPSPKVTWYREDYQIESSIDFQITFQGGIARLMIREAFAEDSGRFTCSAVNEAGT-----VSTSCYLAVQVSEEFDKETTLTEKFATEEKRFVESRDVVMTDTSITEEQAGPGEPAAPFFISKPVVQKLVEGGSVVFECQIGGNPKPHVYWK 1118 HSP 19 Score: 54.6842 bits (130), Expect = 7.880e-7 Identity = 98/497 (19.72%), Postives = 165/497 (33.20%), Query Frame = 3
Query: 249 LYDSPTFQQDLESELVVDEGEPLRLMTRVGPADDGS--LEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATN 1733
+ + P F + LE + GEP+ L +V DG+ + I+W+K+ L + + F L I D G+Y C A++ G S+ V +LP +++ ++ + G P+ E + + L W+KDG LK + + + + L I G Y+C A N LG A + + K L+E + PP F + P + ++ G FK + K++ W + ++ L N + L +L D G Y C + G+ +S L VQ + P FIK+L+ + + + K+ + V WYK+E I S+ ++S++ A L + S EDSGDY A N
Sbjct: 7010 VLEPPYFIEPLE-HVEAAIGEPITLQCKV----DGTPEIRISWYKEHTKLRSAPAYKMQFKNNVASLVINKVDHSDVGEYTCKAENSVGAVASSAVLVIKE--------RKLPPSFARKLKDVHETL---------------------------------GFPVAFECRI--NGSEPLQVSWYKDGELLKDDANLQMSFVHHVATLQILQTDQSHVGQYNCSASNPLGTASSSA-------------KLILSEHEV------------------------PPFF--DLKPVSVDLALGESGSFKCHVTGTAPIKIT--WAKDNREIRPGGNYKMTLVENTATLTVLKVAKGDAGQYTCYASNVAGK-DSCSAQLGVQ--------------------------------------EPPRFIKKLDQSRIVKQDEYTRYECKI--GGSPEIKVLWYKDEVEIQESSKFRMSFEDSVAILEMHSLSVEDSGDYTCEARN 7376
BLAST of Titin vs. Ensembl Mouse
Match: Ttn (titin [Source:MGI Symbol;Acc:MGI:98864]) HSP 1 Score: 109.383 bits (272), Expect = 1.516e-23 Identity = 177/772 (22.93%), Postives = 302/772 (39.12%), Query Frame = 3
Query: 360 EINWFKDGKLLSAGSRFSTIF-DRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPE-EMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLS---------------------QL-----------PNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSL--KLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIV--KTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPI------DQLNEGD-----------------SVTLT--------VKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLAD-SAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLTVIPMVDFRPDLRHI---TPE----NPFKKMAGLKKVEC---TSELNDAFKKSKPTAERIVKIEMGSET---KSKFYKSPDVIETEKLYDQVAA 2426
+I W+KDGK + G R+ F G L I + +D G Y A + G A + + + P + T +P E + I ++ + + M A+ P +S QL P S ++GQ ++ + T WF +G + ++ VI + G +L I P D+G+++ VA+N GK S I+ + E+ Q+K P+F+E + + +++ EG+ KV + W N +I+ R+ + +AL I + D+ Y ++ GR ++ +NV+ E +P+ E +L P+ + EI +LEP+ +Q EGD S+ L ++ P D ++ VEW + + +R+++ + G +L+ +A S DSG ATNK G + I + EK + S L D G QR+++LE G ++ T D +E+ P+ + P + EG A+F +V+G+P P + W ++ L+ + DG I+YL+I C +V V A NP GV + + L + DFR LR PE P K ++KV+ TSE + K + AERI ++ E+ +SKF + + E Y+ + A
Sbjct: 1323 KIAWYKDGKRIRHGERYQMDFLQDGRASLRIPVVLPEDEGIYTAFASNIKGNAICS-GKLYVEPAAPFSAPTYMPTPEAVSRIRSVSPRSLSRSPIRMSPAMSPARMSPARMSPARMSPARMSPGRRLEETDESQLERLYKPVFVLKPASFKCLEGQTARFDLKVVGRPMPE--TFWFHNGQQIVNDYTHKV-VIKEDGTQSLIIVPASPSDSGEWTVVAQNRAGK--------------STISVTLTVEAVEHQIK---------------------PAFVEKL--KNVNIKEGSRLEMKVRATGNPNPDIV--WLKNSDIIVPHKYPRIRIEGTRGEAALKIDSIISQDSAWYTATAINKAGR-DTTRCKVNVEVE---FAEPEP---------ERKLIIPRGTYRAKEIAAP------ELEPLHLRYGQEQWEEGDLYDKEKQQKPFFKKKLTSLRLKRFGPAHFECRLTPIGDPTMVVEWLHDGKPLEAANRLRMINEFGYCSLDYGAAYSRDSGVITCRATNKYGTDHTSATLI--VKDEKSLVEESQLPDGKKGLQRIEELERMAHEGALTGV--TTDQKEKQKPDIVLFPEPVRVLEGETARFRCRVTGYPQPKVNWYLNGQLIRKSKRFRVRYDG-IHYLDIVDCKSYDTGEVKVTAENPEGVTEHKVKLEIQQREDFRSVLRRAPEPKPEFHVHEPGKLQFEVQKVDRPVDTSETKEVVKLKR--AERITHEKVSEESEELRSKFKRRTE----EGYYEAITA 2021 HSP 2 Score: 83.1889 bits (204), Expect = 1.351e-15 Identity = 135/670 (20.15%), Postives = 238/670 (35.52%), Query Frame = 3
Query: 357 LEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTA-----------------------QSNIAHVRINPES--------GVDYLTQLPEE----MMQEINNL----DKNYETGTYHGMEEDKHAAKTI-------PKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLTVIPMVDFRPDLRHI 2228
+ + W KDG+ L R ++ L I T D G Y VA + TA Q + +N + G++ Q+ E + + I+ + ++ + G Y + ++ T+ P+ L +L +T+ G+P + + W+K+ L G + ++D L + +PED Y+C A+N G A T + PE + +P +E Y PP+ + P+ ++ SEG PA F+ + +G L WY + +K + L I P D G+Y V + G+V S TL ++ +SI+ + + I E E+ A P +++EP+ ++ G T +++ A + + +W+K I + I ++L I+ D G+Y A+N+ G ++SC T V E P F +P +T G AKFL VSG P + W+ D + P C+ + + + LE++ + A+N G +A LT+I F +L +
Sbjct: 3092 ITVQWMKDGQELQIADRIKIQKEKYVHRLLIPSTRMSDAGKYTVVAGGNMSTANLFVEGRDVRIRSIKKEVQVIEKQRAVVEFEVNEDDVDAHWYKDGIEINFQVQERHQYVVERRIHRMFISETRHSDAGEYTFVAGRNRSSVTLYVNAPEPPQVLQEL-QPVTVQSGKPARFCAVISGRPQPKI--SWYKEEQLLSTGFKCKFLHDGQEYTLLLIEAFPEDAAVYTCEAKNDYGVATTSASLSVEVPE-------------------VVSPDQEMPVY--------PPAIVTPL--QDTVTSEGRPARFQCQV---SGTDLKVSWYCKDKKIKPSRFFRMTQFEDTYQLEIAEAYPEDEGTYAFVANNAVGQVSST-ATLRLEAPESILHERIGQQI------EMEMKA-------------APVIKRRIEPL-EVALGHLAKFTCEIQGAPN--VRFQWFKAGREIYESDKCSIRSSNYVSSLEILRTQVVDCGEYTCKASNEYG-------SVSC-------------------------------------TATLTVTEAYPPTFLSRPKALTTFVGKAAKFLCTVSGTPVIEIIWQKDGAALSPSPDCRVTDADNKHSLELSNLTVQDRGIYSCKASNKFGADICQAELTIIDKPHFIKELEAV 3659 HSP 3 Score: 76.2554 bits (186), Expect = 1.775e-13 Identity = 78/355 (21.97%), Postives = 130/355 (36.62%), Query Frame = 3
Query: 678 PKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNG 1742
P + + P +T+ G P +E + + L +WFKDG L GSR + + R +L I D G Y+ N +GK+ SC V+ H + ++ PPSF+ + ++ S + GA + + +S W+ +G + + + N+ L + PSDTG+Y CV + G+ ES V L VQ + PSF + + ++ L G S+T T + V+W+K +++G IS + L ++ SGDY + TN G
Sbjct: 6638 PATIVEKPEPMTVTTGNPFTLECVVAGTPE--LSAKWFKDGRELSSGSRHHITFVRNLASLKIPSAEMNDKGLYTFEVENRVGKS---SCTVSVHVSDRVV----------------------------------PPSFVRRL--KDTSATLGASVVLECRV--SGSAPISVGWFLDGNEIISSPKCQSSFADNVCTLTLSSLEPSDTGAYTCVAANVAGQDESSAV-LTVQ--------------------------------------EPPSFEQTPDSVEVL-PGMSLTFTSVIR--GTPPFKVKWFKGSRELVSGEACTISLEDFVTELELLEVEPGQSGDYSCLVTNDAG 6907 HSP 4 Score: 71.2478 bits (173), Expect = 6.603e-12 Identity = 118/561 (21.03%), Postives = 202/561 (36.01%), Query Frame = 3
Query: 180 HALEVVN-QVNKRSL----ASPRRGSD------HLYDSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAG 1829
H+LE+ N V R + AS + G+D + D P F ++LE+ V ++ +D + I W KDG+ L AG + F+ L+I D+G Y C A + +G++ S+ A P P F+ ++ S ++ G+ + L+ + WFK+ L + + + + LDI +D+G YSC A N +G + SC+ VI PPSFI+ ++P +I GA A + I G G +W+ + + +++ + + L I +D G Y CV+ +E G+ G A H L+ + P+F+K+++ L G +VTL V + V W K + I ++++S+ G A L I G Y +A N+ G +Q+ + E KI+ + L+ +AG
Sbjct: 3612 HSLELSNLTVQDRGIYSCKASNKFGADICQAELTIIDKPHFIKELEA---VQSAINKKIHLECQVDEDRKVTITWSKDGQKLPAGKDYKIYFEDKIASLEIPLAKLKDSGTYTCTASNEAGSSSSSAAVAVREP-------------------------------------------PSFVKKVDPSYLMLPGESARLHCKLK--GSPVIQVTWFKNNKELSESNTVRMSFVNSEAILDITDVKVDDSGTYSCEATNDVG---SDSCSTEV-------------------------------------VIKEPPSFIKTLEPADI--VRGANALLQCEIA-GTGP-FEVNWFKDKKQIRSSKKYRLFTQKTFVYLEISSFNSADVGDYECVVANEVGKC----------------------GCVATHLLK-----------------EPPTFVKKVDDFTAL-AGQTVTLQAAVR--GSEPISVMWMKGQEVIKEDGKIKMSFSNGVAVLTIPDVQISLGGKYTCLAENEAG-SQTSVGELIVKEPAKIIERAELIQVTAG 4037 HSP 5 Score: 71.2478 bits (173), Expect = 7.432e-12 Identity = 101/514 (19.65%), Postives = 178/514 (34.63%), Query Frame = 3
Query: 357 LEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPE---------------------------EMMQEINNLDKNYE---------------TGTY-------HGMEEDKHAAKTI-PKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVE-SEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQ 1745
+++W KD + L +G ++ + + + IL D G+Y C A + G+ + P V LT + E+++E +N+ +Y G Y GM+E + P + + P S+ + G +E + + L T+WFKDG L ++ + + L I P D+G YS +N +GK SC V+ + II PPSF + +E + G+ + + +S W+H+G + + + L N AL + +D G Y C+ + G E S P+T + + PSF+++ +P+D L G +VT T V+ V W+K ++ G+R +S L + + SG+Y I +N+ G+
Sbjct: 7422 FQVSWHKDKRELRSGKKYKIMSENLLTSIHILNVDTADIGEYQCKATNDVGSDTCVGSVTMKAPPQFVKKLTDISTIIGKEVQLQTTIEGAEPISVAWFKDKGEIVRESDNIWISYSENIATLQFSRAEPANAGKYTCQIKNDAGMQECYATLSVLEPAAIVEKPESIKVTTGDTCTLECTVSGTPE--LSTKWFKDGKELTSDNKYKISFFNKVSGLKIINVVPGDSGVYSFEVQNPVGK---DSCKVSIQVSDRII----------------------------------PPSFTRKL--KETNGLSGSSVVMECKV--YGSPPISVLWFHDGNEISSGRKYQTTLTDNTCALTVNMLEEADAGDYTCIATNVAGSDECSAPLT----------------------------------------VREPPSFVQKPDPMDVLT-GSNVTFTSIVK--GSPPFTVSWFKGSTELVPGARCNVSLQDSVGELELFDVDTSQSGEYTCIVSNEAGR 7849 HSP 6 Score: 67.781 bits (164), Expect = 7.645e-11 Identity = 108/532 (20.30%), Postives = 176/532 (33.08%), Query Frame = 3
Query: 357 LEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIA---HVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREI---------SVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRV------------------------ESEPVTLN----------------------------VQTEKSIILDPQSKGI--SAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQS 1754
L WFKDGK L++ +++ F L I+ + D+G Y ++ G ++ RI P S L +E N L +G+ ME + G P + WF DG + G + CAL + D GDY+C+A N G E C+ E PPSF++ DP ++ S+ +G+P + + W+ + +R L+ ++ L + S +G Y C++ +E GR + +P+ L TEKS IL+ S + S + E + K +I + P F+KQLEP+ ++ GDS +L ++ A + V WYK + + + ++ + ATL S DSG+Y A N G+ S
Sbjct: 7609 LSTKWFKDGKELTSDNKYKISFFNKVSGLKIINVVPGDSGVYSFEVQNPVGKDSCKVSIQVSDRIIPPSFTRKL--------KETNGL-----SGSSVVME--------------------CKVYGSP-------------PISVLWFHDGNEISSGRKYQTTLTDNTCALTVNMLEEADAGDYTCIATNVAGSDE---CSAPLTVRE-------------------------------------PPSFVQKPDPMDVLTGSNVTFTSIVKGSPPF-------------TVSWFKGSTELVPGARCNVSLQDSVGELELFDVDTSQSGEYTCIVSNEAGRASCTTRLFVKAPAIFVKRLNDYSIEKGKPLILEGTFSGTPPISVTWKKNGINVIASQRCNITTTEKSAILEILSSTVEDSGQYNCYIENASGKDSCSAQILILEPPYFVKQLEPV-KVTVGDSASLQCQL--AGTPEIGVSWYKGDTKLRPTATCKMHFKNNVATLVFTQVDSSDSGEYICRAENSVGEVSS 8038 HSP 7 Score: 67.781 bits (164), Expect = 7.841e-11 Identity = 115/569 (20.21%), Postives = 192/569 (33.74%), Query Frame = 3
Query: 42 RHKYSFIFNLDFIAMTTQESMEYNNNSNFNSVPDEGVIPNGTTMAPHALEVVNQVNKRSLASPRRGSDHLY--DSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNG 1742
+HK+SF N F+ ++ E GT + N+ A + S HL + P F + +S+ V + G ++L VG + I WFKD K L G+ S D +L++ D+G Y+C + GT S P P+F+ + L L GQ E + + + W+ DG + + + + G I+ E++G Y C ARN G T SC++ +LK E PP FI ++P E+ +V W N + +++ + T +M++ L+I PSD G Y C++ +E G S SA AL+ + PSFIK++E + + + + T + A + + W K++ + + IS++ ATL + S + SG Y A N++G
Sbjct: 4726 KHKFSFHDNTAFLEISQLE---------------------GTDSGTYTCSATNK------AGHSQCSGHLTVKEPPYFVEKPQSQDV-NPGTRVQLKALVG--GTAPMTIKWFKDNKELHPGAARSVWKDDTSTILELFSAKAADSGTYICQLSNDVGTTSSKATIFVKEP-------------------------------------------PQFIKKPSPVLVLRNGQSTTFECQVTGTPE--IRVSWYLDGNEITDLRKYGISFVDGLATFQISNARVENSGTYVCEARNDAG---TASCSI--------------------ELKVKE-----------------PPIFIRELEPVEVVKDSDVELECEVM----GTTPFEVTWLKNNKEIRSGKKYTMSEKMSVFYLHITKCDPSDVGEYQCIIANEGG----------------------SCACSARVALK-----------------EPPSFIKKIENVTTVLKSSA---TFQSTVAGSPPISITWLKDDQILEENDNVHISFEDSVATLQVRSVDNGHSGRYTCQAKNESG 5133 HSP 8 Score: 67.0106 bits (162), Expect = 1.166e-10 Identity = 120/599 (20.03%), Postives = 217/599 (36.23%), Query Frame = 3
Query: 354 SLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQ-----LPEEMMQEINNLD----------------------KNYETGTYHGMEEDKHAAK-----------------TIPKFLSQL-------PNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPI--DPREISVSEGAPA----YFKVTIDPGNGQKLSND-----------------------WYHNGEIVKTCSRLTGKLEMNISA-----LNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSI--ILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVT-LTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSE-KINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNIST 1883
+ E+ W+ +G L S+ G + L+IL +D G Y V ++ G A S+ A + + + Y +Q +P+ + E+ + K+ T T + +H A T+ K + L P S+T+ +G+ + + W ++G + +R V + +I+ D G+YS V NS GK E Q + K+ + E +++ +++P E + +T P EP+ P+ +S +E P + V+ P Q L + WY +G+ +K G + + SA L I SD G Y+C + E G ++ Q+ KSI + S+ ++ A K+E E I+ P + L D DSV T+KV + + W K+ I GS+ ++S ++ L I+ + D G Y TN G S K+ I + + S+ Q+ +E+++ +++
Sbjct: 32445 TAEVKWYHNGVELQESSKIHYTNTSGVLTLEILDCQTEDGGTYRAVCTNYKGEA-SDYATLDVTGGAYTTYASQRRDEEVPKSVFPELTKTEAYAVSSFKRTSELEAASSVREVKSQMTETRESLSTYEHYASAEMKSATSEEKSLEEKATVRKIKTTLAARILTKPRSITVHEGESARFS--CDTDGEPVPTVTWLREGQVVSTSARHQVTTTKYKSTFEISSVQASDEGNYSVVVENSDGKQEAQFT--------LTVQKARVIEKAVTSPPRVKSP-EPRVKSPETVKSPKRVKSPEPVTSHPKAVSPTETKPTEKGQHLPVSAPPKITQSLKAEASKDIAKLTCAVESSALCAKEVAWYKDGKKLKE----NGHFQFHYSADGTYELKIHNLSESDCGEYVCEVSGEGGTSKTS-FQFTGQSFKSIHEQVSSISETTKSVQKTAESAEAKKSEPIAPESISSKPVIVTGLR--DTTVSSDSVAKFTIKV--TGEPQPTITWTKDGKAIAQGSKYKLSSNKEEFILEILKTETSDGGLYACTVTNSAGSVSSSCKLTIKAVKDTEAQKVSTQKTSEVTSQKKASAQEEISQKALTS 33022 HSP 9 Score: 67.0106 bits (162), Expect = 1.269e-10 Identity = 98/497 (19.72%), Postives = 163/497 (32.80%), Query Frame = 3
Query: 285 SELV-VDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYH-NGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINI 1769
+EL+ V G+P L V + L+ W+KDG+ L A ++ F L D+G Y + G++ T L ++ +N ++ D A ++P +S WFKDG L R + + G +L+I+ D G+++C A NS+G ++ ++ PPSF+ P V G+ K L+ W+ + E+V S K E + S+L + SD+G+Y C + + G VE D + + +FI++LEP L +GD L KV + + W+ N+ + S+ ++S+ TA L + EDSG+Y A N+ G I I
Sbjct: 4029 AELIQVTAGDPATLEYTVSGTPE--LKPKWYKDGRPLVASKKYRISFKNNIAQLKFYSAELHDSGQYTFEISNEVGSSSCETT------------FTVLDRDIAPLFTKPLRNVDSVVGGACRLDCKIAGSLPMRVS-------------------------------WFKDGKELTASDRYQIAFVEGTASLEISRVDMNDAGNFTCRATNSVGSKDSSGA----------------------------------------LIVQEPPSFV--TKPGSRDVLPGSAVCLKSAFQ--GSAPLTIKWFKGDKELVSGGSCYITK-ETSESSLELYAVKTSDSGTYTCKVSNVAGSVECS---------------------------------------ADLFVKEPATFIEKLEPSQLLKKGDGTQLACKV--TGTPPIKITWFANDRELRESSKHKMSFAESTAVLRLTDVAIEDSGEYMCEAQNEAGSDHCTGIVI 4394 HSP 10 Score: 63.929 bits (154), Expect = 9.954e-10 Identity = 146/714 (20.45%), Postives = 230/714 (32.21%), Query Frame = 3
Query: 291 LVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHH--SDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREI---------SVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVE-------SEPVTL-------NVQTEKSIILD----------------------------------------PQSKGISAIHALEAELNAPKAEMKEDEIITDL--PSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECL-PNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLTV-----IPMVDFRP 2213
+ V GE L +V A L + W+KDGKLL++ + F L IL ++D G Y Q NN+ K+ T D P F +L ++ G L LE S + WF + + G++ + C L + D G Y+CVA N G E C +E PPSF++ +P E+ SV G P FKV W+ + +R E ++ L + S +G Y CV+ + G+ EP T +V+ KSIIL+ +KG + ++ E E A + D +++ L P F+ +LEP++ + GDSV+L +V A + V W+K + + + + + ATL DSG+Y +A N G S+ I F+ +QE L P+F + +I G P +++G + W D VL+ D + ++ L+I + + +A+NPLG A A LT P D +P
Sbjct: 5707 MTVTVGETCALECKV--AGTPELSVEWYKDGKLLTSSQKHKFSFYNKISSLKILSVEKEDAGTYT-----------------------------------FQVQNNVGKSSCTAVV-----DVSDRMVPPSFTRRLKDT-----GGVLGTSCILECKVAGSSPISIAWFHEKTKIVSGAKYQTTFSDNVCTLQLNSLDSSDMGSYTCVAANVAGSDE---CRALLTVQE-------------------------------------PPSFVKEPEPLEVLPGKNITFTSVIRGTPP-FKV------------GWFRGARELVKGNRCNIYFEDTVAELELFNIDISQSGEYTCVVSNNAGQASCTTRLFVKEPATFVKKLSDHSVEPGKSIILEGTYTGTLPISVTWKKDGVSITPSERCNIVTTEKTCILEILSSTKGDAGHYSCEIENEAGRDAC--DALVSTLEPPYFVTELEPLEA-SVGDSVSLQCQV--AGTPEITVSWFKGDTKLRSTPEYRTYFTNNVATLVFNKVSINDSGEYTCMAENSIGTAASKTI----------------------------------------FR----IQERQLPPSFARQLKDIEQTVGLPVTLTCRLNGSAPIQVCWYRDGVLLRDDENLQMSFVDNVATLKILQTDLSHSGQYSCSASNPLGTASSTARLTAREPKKSPFFDIKP 6271 HSP 11 Score: 61.2326 bits (147), Expect = 7.805e-9 Identity = 96/466 (20.60%), Postives = 160/466 (34.33%), Query Frame = 3
Query: 363 INWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNS--LYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQS 1754
++WFK L G+R + L++ +G+Y C+ + +G A T+L K F+ +L N ++ +G+PL +E S+ W K+G+++ R ++ L+I ED+G Y+C N+ GK SC+ +Q+ LE PP F++ ++P +++V + A ++ P ++ WY ++ + + N++ L SD+G YIC + G V S L VQ +K PSF +QL + + +V L V E A S + V WYK+ + + +Q S+ ATLNI SG Y ATN G S
Sbjct: 7800 VSWFKGSTELVPGARCNVSLQDSVGELELFDVDTSQSGEYTCIVSNEAGRASCT---------------TRL----------------------------FVKAPAIFVKRL-NDYSIEKGKPLILEGTFSGTPPISVT--WKKNGINVIASQRCNITTTEKSAILEILSSTVEDSGQYNCYIENASGK---DSCS--------------------AQILILE-----------------PPYFVKQLEPVKVTVGDSASLQCQLAGTP----EIGVSWYKGDTKLRPTATCKMHFKNNVATLVFTQVDSSDSGEYICRAENSVGEVSSS-TFLTVQEQKL-----------------------------------PPSFSRQLRDVQE-----TVGLPVVFECAVSGSEPISVSWYKDGKPLKDSPNIQTSFLDNIATLNIFKTDRSLSGQYSCTATNPIGSASS 8134 HSP 12 Score: 60.4622 bits (145), Expect = 1.457e-8 Identity = 63/281 (22.42%), Postives = 119/281 (42.35%), Query Frame = 3
Query: 108 YNNNSNFNSVPDEGVIPNGTTMAPHALEVVNQVNKRSLASPR--RGSDHLYDSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYE---TGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDR--GYCALDIAFCYPEDNGDYSCVARNSLGK 929
Y + + + GV +G+T+ EV+ + + R ++ PT L++ V+ EGE + L + + S ++ W+++ + + F F G L I +D+G + C A + +GT ++ ++ + D T L E+ E ++ + T T E+ P F+S+ P L++G + E + + + W K G+ L G R V Y++ G C L I+ + +D G+Y+ V RN G+
Sbjct: 893 YKSQAGIEVKKEVGVSISGSTVREEHFEVLRGREAKVTETARVPAPAEVPVTPPTLVSGLKNVTVI-EGESVTLECHI--SGYPSPKVTWYREDYQIESSIDFQITFQGGIARLMIREAFAEDSGRFTCSAVNEAGTVSTS-CYLAVQVSEEFDKETTLTEKFATEEKRFVESRDVVMTDTSITEEQAGPGEPAAPFFISK-PVVQKLVEGGSVVFECQIGGNPKPHVY--WKKSGVPLTTGYRYKVSYNKQTGECRLVISMTFADDAGEYTIVIRNKHGE 1166 HSP 13 Score: 57.7658 bits (138), Expect = 7.945e-8 Identity = 78/331 (23.56%), Postives = 136/331 (41.09%), Query Frame = 3
Query: 978 ITKSNLTESSISQLKSLENPGEEYARYNDTSVIPM-PPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITD---------LPSFIKQLEPIDQ-LNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAIS--EDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRL-KDLEEDLASGNISTFQNTEDVQEECLPNF 1928
I+ S + E L+ E E AR + +P+ PP+ + + + ++V EG + I K++ WY +++ + I+ L I D+G + C +E G V S L VQ + D ++ E + + D IT+ P FI + P+ Q L EG SV ++ + +V W K+ + G R ++SY++ T +V +++ +D+G+Y ++ NK+G+T +AS+ L + A Y+ L K +E L +STF V E P F
Sbjct: 909 ISGSTVREEHFEVLRGREAKVTETARVPAPAEVPVTPPTLVSGL--KNVTVIEGESVTLECHISGYPSPKVT--WYREDYQIESSIDFQITFQGGIARLMIREAFAEDSGRFTCSAVNEAGTV-STSCYLAVQVSEE--FDKETTLTEKFATEEKRFVESRDVVMTDTSITEEQAGPGEPAAPFFISK--PVVQKLVEGGSVVFECQI--GGNPKPHVYWKKSGVPLTTGYRYKVSYNKQTGECRLVISMTFADDAGEYTIVIRNKHGET----------------SASASLLEEADYEALVKTQQEMLYQTQMSTFIQEPKVG-EIAPGF 1211 HSP 14 Score: 56.6102 bits (135), Expect = 1.726e-7 Identity = 112/481 (23.28%), Postives = 187/481 (38.88%), Query Frame = 3
Query: 1110 PREISVSEGAPAYFKVTIDPGNGQKLSN-DWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQT----EKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLE--PIDQ-----LNEGDSVTLTVKVEPANDNSLY-------------VEWYKNENHIINGSRMQISYDR-GTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVT----ASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVHVA-ATNPLGVAKEEATLTVIPMVDFRPDLRHITPENPFKKMAGLKKVECTSE----LNDAFKKSKPTAERIVKIEMGS-ETKSKFYKSPDVIETEKLYDQVAAGLRKNI 2444
PR I+V EG A F D G+ + W G++V T +R S I SD G+Y V+ + G+ E++ TL VQ EK++ P+ K + +PK +K E +T P + E P ++ ++ +T ++K E + D + V WYK+ + Q Y GT L I + D G+Y + + G +++ F G+ + SS+ + Q+ + E S I+ E + + P + T AKF +KV+G P PT+ W D + S K ++ LEI + +E D ++ TN G LT+ + D +K++ K E TS+ + + T+E I E+ S ET + ++ V+ E++ AA L K+I
Sbjct: 32610 PRSITVHEGESARFSCDTD---GEPVPTVTWLREGQVVSTSARHQVTTTKYKSTFEISSVQASDEGNYSVVVENSDGKQEAQ-FTLTVQKARVIEKAVTSPPRVKSPEPRVKSPETVKSPK-RVKSPEPVTSHPKAVSPTETKPTEKGQHLPVSAPPKITQSLKAEASKDIAKLTCAVESSALCAKEVAWYKDGKKLKENGHFQFHYSADGTYELKIHNLSESDCGEYVCEVSGEGGTSKTS----FQFTGQSFKSIHEQVSSISETTKSVQKTAESAEAKKSEPIAP----ESISSK--PVIVTGLRDTTVSSDSVAKFTIKVTGEPQPTITWTKDGKAIAQGSKYKLSSNKEEFILEILK-TETSDGGLYACTVTNSAGSVSSSCKLTIKAVKD-----------TEAQKVSTQKTSEVTSQKKASAQEEISQKALTSEEIKMSEVKSHETLAIKEEASKVLIAEEVKRSAAASLEKSI 33063 HSP 15 Score: 56.6102 bits (135), Expect = 1.847e-7 Identity = 107/498 (21.49%), Postives = 161/498 (32.33%), Query Frame = 3
Query: 279 LESELVVDEGEPLRLMTRVGPADDGSLE----------INWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNG 1742
LE V + EP+++ D SL+ ++W+K L + F L D+G+Y+C A++ G S+ T+ ++E K P F QL + + G P+ E + S+ W+KDG LK I + L+I +G YSC A N +G A + + K LTE PP F P+ P + V E A VT Q + W + +++ N + L I+ D+G Y C +E G+ + A+LN +KE I PSF K+L + EG+S L +V A + V WYKN I S +I + L + A D+G Y ATN G
Sbjct: 7952 LEPPYFVKQLEPVKVTV----GDSASLQCQLAGTPEIGVSWYKGDTKLRPTATCKMHFKNNVATLVFTQVDSSDSGEYICRAENSVGEVSSS------------------------------------TFLTVQEQKLP----PSFSRQLRDVQETV-GLPVVFECAVSGSEPISV--SWYKDGKPLKDSPNIQTSFLDNIATLNIFKTDRSLSGQYSCTATNPIGSASSGA-------------KLILTEGK------------------------NPPFFDIPLAPMDAVVGESADLECHVT----GTQPIKVTWAKDNREIRSGGNYQISYLENSAHLTIVKVDKGDSGQYTCYAVNEVGK----------------------------DSCTAQLN-----IKERLI---PPSFTKKLSETVEETEGNSFKLEGRV--AGSQPITVAWYKNNVEIHPTSNCEIMFKNNALLLQVKRASMADAGLYTCKATNDAG 8323 HSP 16 Score: 56.6102 bits (135), Expect = 1.976e-7 Identity = 96/543 (17.68%), Postives = 180/543 (33.15%), Query Frame = 3
Query: 249 LYDSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSK---------------------------------GISAIHALEAE--------LNAPKAEMKEDEIITDL--PSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQT 1748
L + P+F + +E+ V + T G + I W KD ++L F+ L + +G Y C AK+ SG + L Q P +++++ ++D + + P+ +E + + L +W KDG + + ++ + I +D+G Y+ N G + SC+ Y R D + PPSF + + + + KV+ +S W+ +G+ + T ++ N +L + DT +Y C + + G + L V+ S ++ P+ + G LE +++ K ++ D+ P F+K+LE + GDS L K+ + V WY+NE+ + + Q+++ A + + S +EDSGD+ A N G T
Sbjct: 5050 LKEPPSFIKKIENVTTVLKSSATFQSTVAG---SPPISITWLKDDQILEENDNVHISFEDSVATLQVRSVDNGHSGRYTCQAKNESGIERCYAF-----------LLVQEPAQIIEKAKSVD---------------------------------VTEKDPVTLECVVAGTPE--LKVKWLKDGKQIVPSRYFSMSFENNVASFRIQSVMKQDSGQYTFKVENDFG---SSSCDA-------------------------------YLRVLDQDI---PPSFTKKLTKMDKVLGSSIHMECKVS----GSLPISAQWFKDGKEISTSAKYRLVCHENTVSLEVSNLELEDTANYTCKVSNVAGDNACSGI-LTVKEPPSFLVKPERQQAIPDSTVEFKAVLKGTPPFKIKWLKDDVELVSGPKCFIGLEGSTSFLNLYSVDSSKTGQYTCQVTNDVEPPKFVKKLEASKIIKAGDSARLECKI--TGSPEIQVVWYRNEHELTASDKYQMTFIDSVAVIQMNSLGTEDSGDFICEAQNPAGST 5499 HSP 17 Score: 56.225 bits (134), Expect = 2.419e-7 Identity = 64/258 (24.81%), Postives = 107/258 (41.47%), Query Frame = 3
Query: 258 SPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSA----GSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQL 1019
+P F Q L+S +VV EG V + E++WF+DG+++S G + S F G L I + ++G Y A + SG A S E++ A+T P SQ S+T+ QG + +++ + + V ++++DG ++ + + +L IA YPED+G YS A NS+G+A T + + EE + K T S +Q+
Sbjct: 5 APMFTQPLQS-VVVLEGSTATFEAHV--SGSPVPEVSWFRDGQVISTSTLPGVQIS--FSDGRARLMIPAVTKANSGRYSLRATNGSGQATST-------------------AELLV----------------------TAETAPPNFSQRLQSMTVRQGSQVRLQVRV--TGIPTPVVKFYRDGAEIQSSLDFQISQEGDLYSLLIAEAYPEDSGTYSVNATNSVGRA-TSTAELVVQGEEVVPAKKTKTIVSTAQI 213 HSP 18 Score: 55.0694 bits (131), Expect = 5.817e-7 Identity = 48/187 (25.67%), Postives = 81/187 (43.32%), Query Frame = 3
Query: 1476 EIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEG----EKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEWR 2024
E+ P+ + L+ + + EG+SVTL + + S V WY+ + I + QI++ G A L I A +EDSG + A N+ G ++ SC+ E+ ++L A ++ D+ + S + E P F KP+ EGG F ++ G P P + W+
Sbjct: 940 EVPVTPPTLVSGLKNVTVI-EGESVTLECHI--SGYPSPKVTWYREDYQIESSIDFQITFQGGIARLMIREAFAEDSGRFTCSAVNEAGT-----VSTSCYLAVQVSEEFDKETTLTEKFATEEKRFVESRDVVMTDTSITEEQAGPGEPAAPFFISKPVVQKLVEGGSVVFECQIGGNPKPHVYWK 1118 HSP 19 Score: 54.6842 bits (130), Expect = 7.880e-7 Identity = 98/497 (19.72%), Postives = 165/497 (33.20%), Query Frame = 3
Query: 249 LYDSPTFQQDLESELVVDEGEPLRLMTRVGPADDGS--LEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATN 1733
+ + P F + LE + GEP+ L +V DG+ + I+W+K+ L + + F L I D G+Y C A++ G S+ V +LP +++ ++ + G P+ E + + L W+KDG LK + + + + L I G Y+C A N LG A + + K L+E + PP F + P + ++ G FK + K++ W + ++ L N + L +L D G Y C + G+ +S L VQ + P FIK+L+ + + + K+ + V WYK+E I S+ ++S++ A L + S EDSGDY A N
Sbjct: 7010 VLEPPYFIEPLE-HVEAAIGEPITLQCKV----DGTPEIRISWYKEHTKLRSAPAYKMQFKNNVASLVINKVDHSDVGEYTCKAENSVGAVASSAVLVIKE--------RKLPPSFARKLKDVHETL---------------------------------GFPVAFECRI--NGSEPLQVSWYKDGELLKDDANLQMSFVHHVATLQILQTDQSHVGQYNCSASNPLGTASSSA-------------KLILSEHEV------------------------PPFF--DLKPVSVDLALGESGSFKCHVTGTAPIKIT--WAKDNREIRPGGNYKMTLVENTATLTVLKVAKGDAGQYTCYASNVAGK-DSCSAQLGVQ--------------------------------------EPPRFIKKLDQSRIVKQDEYTRYECKI--GGSPEIKVLWYKDEVEIQESSKFRMSFEDSVAILEMHSLSVEDSGDYTCEARN 7376 HSP 20 Score: 51.2174 bits (121), Expect = 8.791e-6 Identity = 58/250 (23.20%), Postives = 101/250 (40.40%), Query Frame = 3
Query: 228 PRRGSDHLYDS--PTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDR-GFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLH----IEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVT 956
P HL S P Q L++E D +L V + + E+ W+KDGK L F + G L I E D G+YVC GT++++ + +S + ++ + E ++ K E+ E K + P+ +S P +T ++ + + ++ + W KDG ++ GS+ + ++ L+I D G Y+C NS G + SC +T
Sbjct: 32748 PTEKGQHLPVSAPPKITQSLKAEASKDIA---KLTCAVESSALCAKEVAWYKDGKKLKENGHFQFHYSADGTYELKIHNLSESDCGEYVCEVSGEGGTSKTSFQFTGQSFKSIHEQVSSISETT----KSVQKTAESA------EAKKSEPIAPESISSKPVIVTGLRDTTVSSDSVAKFTIKVTGEPQPTITWTKDGKAIAQGSKYKLSSNKEEFILEILKTETSDGGLYACTVTNSAGSV-SSSCKLT 32983
BLAST of Titin vs. UniProt/SwissProt
Match: sp|Q9I7U4|TITIN_DROME (Titin OS=Drosophila melanogaster OX=7227 GN=sls PE=1 SV=3) HSP 1 Score: 257.684 bits (657), Expect = 8.380e-70 Identity = 201/703 (28.59%), Postives = 330/703 (46.94%), Query Frame = 3
Query: 288 ELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGT--YHGMEEDKHAAKTI---------PKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQ-LKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQ-SKGIS---AIHALEAELNAPKAE--MKEDEII----TDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEK-INISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLTVIPMVDFRPDLRHITPENPFK----KMAGLKKVECTSELNDAFKKSK 2315
++V+ E R+ P +D S+ ++WF +GK L AGSR TI D GF++L+I + D+G Y C A + G A + +++ G+ QLP N+ TGT +EE H + + PKF ++ ++L + +G P+H + +E D ++ EWF +G + GSR+ + D G+ ALD+ + Y D+G+Y+C A N G A T S VTC + +I+ +S L E S+ LK LE G + PP F I ++V E F+ ++P L +WY NG+ + + R +M +L+ILY D+G Y+C + +G + T++ + +I+L Q +G+ A+ +EA + +E + ED++ P F+ Q++ L E ++ P D ++ VEW+ N +++ +R Q YD G +N EDSG+Y ATN G+ ++ I +S G IV S L A R++++E AS + + D + P F K T EG PA+F V+V+G P P + W I+ VV S K NDG + +L++ + + KV V A N +G + L V+ D D R++ +P ++A +K +EL F + K
Sbjct: 3547 DMVIGENALAHFECRLQPINDPSMRVDWFHNGKALWAGSRIKTINDFGFVILEIAGCYQRDSGLYTCKATNKHGEATVS-CKLQVKGRQGIVMEPQLP-----------SNFRTGTESLQKLEETMHKREELVTEDEQPNPPKFTEEIKDNLDVPEGGPIHFDCRVEPVGDPTMRIEWFYNGHVMATGSRVHQLNDFGFIALDVDYIYARDSGEYTCRATNKWGTA-TTSAKVTCKGKHNIVYESQLPEGMTSEKLKELER-GRIPEAPKVVEEVFGPPKFTTQIT--SVTVDEAEAVRFECQVEPKTDPSLRVEWYRNGKPLPSGHRYRNIFDMGFVSLDILYVYGEDSGEYVCRAINNYGEDRTR-ATVSCKKLPTILLQNQVPRGMKRSDALTQMEATIKKYTSEVHLTEDDLFDPDRKQPPRFVTQIKEQLTLTEMAVTKFECQLAPVGDPNMKVEWFFNGKPLLHKNRFQPIYDFGYVAMNFGWVYPEDSGEYVCRATNLYGKDETRAIIKVSGKPG--IVYDSQLPAHMQSIDRIREME---ASWQVVPDEVDPDAKPRTKPVFVSKLEPQTVEEGDPARFCVRVTGHPRPRVMWLINGHTVVHGSRYKLTNDG-MFHLDVPKTRQYDTGKVEVIARNSVGESIATTELKVVARSD---DYRNVLKNSPRPWYDYELAAYQKERQENELEKVFDERK 4223 HSP 2 Score: 233.417 bits (594), Expect = 7.286e-62 Identity = 190/684 (27.78%), Postives = 319/684 (46.64%), Query Frame = 3
Query: 297 VDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKA-ETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVT---LNVQTEKSIILDP-QSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSA---GYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAG--------EGGPAKFLVKVSGFPTPTLEWRID-----DVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLTVIPMVDFRPDLRHITPENPFKKMAGLKKVECTS 2285
V E + + R+ P D L + W ++G+ + A +R +T+ D G++ L++ Y +D+G Y C A + G A ++ A + + ++ + TQ E M +I+ L+ + Y EE+++ T P F+++L L++GQ H E +E + D +L EWF +G L G R YD G+ ALDI Y ED+G+Y+C N+LG+A + NVT S I E ++++++ LE+ + ++ P F P+ R V+EGAP + + T+ P N + +WY NG ++T R + AL+ILY DTG+Y+C + G E VT +NV K++ LD ++ + I LE PK ++E P F+ L ++ L EG+ L +VEP ND +L +EW+ N + G R + ++D G L+I+ ED+G Y ATN+ G E +N +C +++ S++ D+ ++++ LE + + + + + P + TA EG A F +V+ P L RI+ L T++ G ++ L+I + V A N LG A L VIP D +H PE + ++K+E T+
Sbjct: 1702 VAENQAVHFEARLIPVGDPKLTVEWLRNGQPIEASNRTTTMHDFGYVALNMKYVNPEDSGTYTCRAVNELGQAVTS-ASLIVQSKTSIQLETQH-EAAMHKIHQLEDH---SRYQRREEEEYTVTTAPVFVTKLIGPSNLVEGQSAHYECRIEPYPDPNLKVEWFHNGKPLSTGHRFRTTYDFGFAALDILTVYAEDSGEYTCRVTNNLGEAINSIVLNVTSR---SSIIHETQHEEALTKIQHLEDTSRFQRKTDEEQFHAERPQFGRPL--RNAKVNEGAPVHLEATLIPVNDPTMKVEWYCNGRPIQTGHRFKTTYDFGFVALDILYAHAEDTGTYMCKAKNAIG----EAVTTCAVNVTANKTLDLDTLDAQRLEKIRQLETYAPPPKPVVEEK---GQKPIFLTPLSNLEHLKEGEHAHLECRVEPINDPNLKIEWFCNGKQLPTGHRYRTTHDFGYVALDILYVYGEDTGTYICKATNQLG----EAVN-TC--NVRVLNRRSMILDTQHPDALEKIQKLESKVPNARTE------------VGDAPISPPHFTAELRGSTEIYEGQTAHFEAQVAPVHDPNL--RIEFYHNGKPLPSASRFHITFDFGYVS-LDITHAVAEDAGEYSVRAVNALGQAVSSTNLRVIPRGTIISDTQH--PEG----LEKIRKLESTA 2340 HSP 3 Score: 218.394 bits (555), Expect = 5.078e-57 Identity = 182/669 (27.20%), Postives = 315/669 (47.09%), Query Frame = 3
Query: 243 DHLYDSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESS--ISQLKSLEN--PGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSK-GISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAG---YQRLKDLEEDLASGNISTFQNTEDVQE--ECLPNFDVKPMN----ITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLTVIPMVDF 2207
D +Y P F L++ + EG + + R+ P D ++ + W+ +G+ L+A +R +++F GFI LD+L + D+G+Y+C + SG A+S A + + ++ +Q P +Q IN L+ +Y Y E P+F+ L + +G+ +H E + +D S+ EW+KDG+ + SRI I++ GY +L+I ED G Y+ A N +G+A +QS ++ H + + E I +++ LE+ ++ + + PP F PI ++ + E A A+F+ ++P + +W +G+ ++ SR+T AL I D G+Y C ++ G+ ++ L V ++ I+ + Q G+ I LE + E +E+ IT P F+ L+ ++ EG +VEP +D L +EWY N I +R+Q YD G L+I +ED+G Y ++A NK G+ Q + I + T SS+ S Y++ ++LE N + D++E + P F V P++ I G+ + ++ G PT +EW + V S +TY D L+I + + + V ATN LG A A + VI D
Sbjct: 612 DRVYPPPQFIIPLQN-VQQTEGGRVHMEARIEPVGDPTMVVEWYLNGRPLAASARATSVFKFGFIALDLLSIMGHDSGEYMCRVTNASGVAESR-AILSVVQRPSIEQSSQNPN-SLQYINQLE-DY--SRYQRTESIDEQLNQAPQFIRPLRDLGEFEEGKNVHFEAQVTPVNDPSMRVEWYKDGLPITASSRITAIFNFGYVSLNILHLRAEDAGTYTVRAVNRIGEAISQS-SIRVHSRSQVTADLGIPEQQRYIEKVEELEDYRKSQQRRHVQEAAEAIAPPQFKTPIQ-NQLDLREHAHAHFEARLEPVGDSTMRVEWLKDGQPLEASSRITTYHNFGYVALTIKQLTIYDAGTYTCRAYNAMGQ-DTTVAQLTVISKNEIVSESQHPGGLQKIQHLEDSSRYGRRE-EEETYITQAPRFLGPLKGTTKILEGQRAHFEARVEPQSDLGLVIEWYHNGRSITAANRIQTYYDFGYVALDISQVRAEDAGVYLVVARNKLGEAQQQATMI-------VETRSSIDTSSMHRGLYEKTQNLE------NKPFVEPQYDIEEISKSKPVF-VTPLSDPKPIHDGKNIHLECRLEPMGDPTMRVEWFHNGRPVTVGSRFRTYYDFGFVALDIIKATAADSGEYTVRATNHLGTAHTSACVRVIDHTDV 1256 HSP 4 Score: 218.009 bits (554), Expect = 8.328e-57 Identity = 174/591 (29.44%), Postives = 279/591 (47.21%), Query Frame = 3
Query: 303 EGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEM---MQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNL---TESSISQLKSLENPGE---EYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQ-----SKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECL--PNFDVKPMNITAGEGGPAKFLVKVSGFPTPTL--EW 2021
EG+ R+ P D L + W+ +GK L G RF T D G ++LDILY E+++G Y A++ G + A ++ +S + +QLP M +++I NL+ + EE K A P F L N L +G+ H E + D L EW+ +G LK GSR D G+ L+I+ YPED+G+YSC A N G+A T + + + SII +S L E +I ++ LE G E+ +DT PP FI P ++ + E A A+F+ + P N + DW+HNG+ + SR+ + L I D+G Y C ++HG + L V+ + I+++PQ G ++ LE ++ + + EDE + P F ++++ + EG + +VEP D ++ +EW+ N + + GSR+ D G L++ + DSG+Y ATNK G T + ++C +G+ + S L + ++LK+LE G I V EE P F + ++T E +F +V P+L EW
Sbjct: 3277 EGQSAHFEARLTPITDPDLVVEWYFNGKKLPHGHRFRTFHDFGIVILDILYCYEENSGVYEARARNKYGEDVTR-ASLKCASKSSLILDSQLPRGMEGGLEKIANLEYSMVRTREETTEETKGKA---PVFTVPLENIENLREGENAHFEARITPADDPKLKVEWYWNGRPLKAGSRFRTFCDFGFVILEISPVYPEDSGEYSCRAINEYGEAVT-TATMKIQGKRSIIMESQLPKGMEGTIDRIAELEGLGSRSTEFVPDDDTG---KPPEFI--TSPFDMVIGENALAHFECRLQPINDPSMRVDWFHNGKALWAGSRIKTINDFGFVILEIAGCYQRDSGLYTCKATNKHGEA-TVSCKLQVKGRQGIVMEPQLPSNFRTGTESLQKLEETMHKREELVTEDE-QPNPPKFTEEIKDNLDVPEGGPIHFDCRVEPVGDPTMRIEWFYNGHVMATGSRVHQLNDFGFIALDVDYIYARDSGEYTCRATNKWG-TATTSAKVTC-KGKHNIVYESQLPEGMTSEKLKELE----RGRIP---EAPKVVEEVFGPPKFTTQITSVTVDEAEAVRFECQVEPKTDPSLRVEW 3846 HSP 5 Score: 217.624 bits (553), Expect = 9.879e-57 Identity = 191/640 (29.84%), Postives = 288/640 (45.00%), Query Frame = 3
Query: 339 PADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRIN--PESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTI--PKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNL---TESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQ-SKG----ISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQ-LNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQ-TQSEKINISCFEGEKIVTASSLLAD-SAGYQRLKDLEEDLASGNISTFQNTEDVQ-------EECLPNFDVKPMNITAGEGGPAKFLVKVS--GFPTPTLEWRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLT 2186
P +D L++ W+ +GKL+ +R T+ D G++VLDI Y + D+G+YVC A + G + +N GV Y + P+ +Q I L+ G + D A PKF++Q+ + L++GQ H E L +D LV EW+ +G L G R +D G LDI +CY E++G Y ARN G+ T++ ++ C + S+I S L E + ++ +LE P F P++ E ++ EG A+F+ I P + KL +WY NG +K SR + L I P D+G Y C +E+G + T+ +Q ++SII++ Q KG I I LE L + E D+ P FI P D + E +++P ND S+ V+W+ N + GSR++ D G L I DSG Y ATNK+G+ T S K+ + +G IV L ++ G + L+ LEE + TED Q EE N DV EGGP F +V G PT +EW + ++ S ND L+++ + ATN G A A +T
Sbjct: 3156 PVNDPDLKVEWYHNGKLMRHSNRIKTVSDFGYVVLDISYLQDHDSGEYVCRAWNKYG---EDFTRTTLNCGGRGGVFYDSLQPD-SLQRIRELE------CPQGQQADTSAPLVAEPPKFITQIVDVTKLVEGQSAHFEARLTPITDPDLVVEWYFNGKKLPHGHRFRTFHDFGIVILDILYCYEENSGVYEARARNKYGEDVTRA-SLKCASKSSLILDSQLPRGMEGGLEKIANLEYSMVRTREETTEETKGKAPVFTVPLENIE-NLREGENAHFEARITPADDPKLKVEWYWNGRPLKAGSRFRTFCDFGFVILEISPVYPEDSGEYSCRAINEYGEAVTT-ATMKIQGKRSIIMESQLPKGMEGTIDRIAELEG-LGSRSTEFVPDDDTGKPPEFI--TSPFDMVIGENALAHFECRLQPINDPSMRVDWFHNGKALWAGSRIKTINDFGFVILEIAGCYQRDSGLYTCKATNKHGEATVSCKLQVKGRQG--IVMEPQLPSNFRTGTESLQKLEETMHKREELV---TEDEQPNPPKFTEEIKDNLDV-------PEGGPIHFDCRVEPVGDPTMRIEWFYNGHVMATGSRVHQLNDFGFIALDVDYIYARDSGEYTCRATNKWGTATTSAKVT 3767 HSP 6 Score: 212.231 bits (539), Expect = 6.193e-55 Identity = 148/513 (28.85%), Postives = 253/513 (49.32%), Query Frame = 3
Query: 258 SPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTES------SISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQ-SKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISC 1775
+P F LE+ + EGE R+ PADD L++ W+ +G+ L AGSRF T D GF++L+I +D+G+Y C A + G A + A ++I + + +QLP+ M I+ + + G+ P+F++ P + + + H E L+ +D S+ +WF +G +L GSRI I D G+ L+IA CY D+G Y+C A N G+A T SC + + I+ + L + S+ +L+ + EE ++ P PP F E I + V EG P +F ++P + +W++NG ++ T SR+ + AL++ Y D+G Y C ++ G + + + + +I+ + Q +G+++ E E K E + P F Q+ + ++E ++V +VEP D SL VEWY+N + +G R + +D G +L+I+ EDSG+Y A N G+ ++ + +SC
Sbjct: 3400 APVFTVPLENIENLREGENAHFEARITPADDPKLKVEWYWNGRPLKAGSRFRTFCDFGFVILEISPVYPEDSGEYSCRAINEYGEAVTT-ATMKIQGKRSIIMESQLPKGMEGTIDRIAELEGLGSRSTEFVPDDDTGKPPEFITS-PFDMVIGENALAHFECRLQPINDPSMRVDWFHNGKALWAGSRIKTINDFGFVILEIAGCYQRDSGLYTCKATNKHGEA-TVSCKLQVKGRQGIVMEPQLPSNFRTGTESLQKLEETMHKREELVTEDEQ---PNPPKFTEEIKDN-LDVPEGGPIHFDCRVEPVGDPTMRIEWFYNGHVMATGSRVHQLNDFGFIALDVDYIYARDSGEYTCRATNKWGTATTS-AKVTCKGKHNIVYESQLPEGMTSEKLKELERGRIPEAPKVVEEVFGPPKFTTQITSVT-VDEAEAVRFECQVEPKTDPSLRVEWYRNGKPLPSGHRYRNIFDMGFVSLDILYVYGEDSGEYVCRAINNYGEDRT-RATVSC 3902 HSP 7 Score: 205.682 bits (522), Expect = 7.224e-53 Identity = 202/709 (28.49%), Postives = 313/709 (44.15%), Query Frame = 3
Query: 12 HNGAIFKCITRHK--YSFIF-NLDFIAMTTQESMEYNNNSNFNSVPDEGVIPNGTTMAPHALEVVNQV-------NKRSLA-------SPRRGSDH----LYDSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTI-PKFLSQLPNSLT-LIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSK-GISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNI------STFQNTEDVQEE----CLPNFDVK---PMNITAGEGGPAKFLVKVSGFPTPTL--EW 2021
HNG +R + Y F F LD I T +S EY + + GT + V++ N++SL S RR + +P F + L + + EG + L R+ P D S+ I WF +GK + G RF ++ ++ LD+L D+G Y C A++ G A ++ + VRI ++ + TQ E +Q+I L E T H E I P+FL+ P SLT +G H E +E +D +L EWFK+G + +G R I+D GY ALDI ED+G Y+C A N +G ETQ + C E I+T + E+ + Q+ LE+ R P F + + + + E A+F+ + P + + +WYHN +K+ SR T AL+I+ TLP D G+Y C ++ G + V + V T+KSI L+ Q + + + LE + + +DE ++ P F + + ++ E +V ++ P D L VEW +N I +R +D G LN+ EDSG Y A N+ GQ VT++SL+ S +L + + + A I S +Q E+ EE P F K P N+ EG A + ++ +P P L EW
Sbjct: 1196 HNGRPVTVGSRFRTYYDFGFVALDIIKATAADSGEYTVRATNHL---------GTAHTSACVRVIDHTDVVTETQNEQSLEQIQLLEDSRRRHHQEEDITIMQAPQFTRGLHN-IETIEGTNVHLECRLQPVGDPSMRIEWFVNGKPVKTGHRFRPAYEFDYVALDLLGCYAIDSGVYTCQARNQLGEAVTSCS-VRIIAKNDLILETQN-ESGLQKIQYL----EDSTRHRRSEFVDEVVNIRPRFLTH-PKSLTNTREGGHAHFECKIEPVTDPNLKVEWFKNGRPITVGHRFRPIHDFGYVALDIVHLIAEDSGVYTCRAVNLIGSDETQ-VELQCRSGEQIVTVTQ-NEAGLEQIHYLEDRSRYTRREEIDESTKQAPVFTTSL--KNVEIKENQRAHFECRLIPVSDPSMRVEWYHNNLPLKSGSRFTETNNFGFVALDIMSTLPEDAGTYTCRAYNAVGEAITSAVAV-VHTKKSIYLESQHETALPRLQHLEDGSKRQRISV-QDEFVSQAPVFTMPVRDV-RVAENQAVHFEARLIPVGDPKLTVEWLRNGQPIEASNRTTTMHDFGYVALNMKYVNPEDSGTYTCRAVNELGQA---------------VTSASLIVQSKTSIQL-ETQHEAAMHKIHQLEDHSRYQRREE--EEYTVTTAPVFVTKLIGPSNLV--EGQSAHYECRIEPYPDPNLKVEW 1860 HSP 8 Score: 204.527 bits (519), Expect = 1.571e-52 Identity = 162/603 (26.87%), Postives = 284/603 (47.10%), Query Frame = 3
Query: 261 PTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSI-----SQLKSLENPGEEYARYNDTSVIPMPPSFIEPI-DPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQS-KGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADS---AGYQRLKDLEEDLASGNISTFQNTEDVQEECL---PNFDVKPMNIT-AGEGGPAKFLVKVSGFPTPTL--EW 2021
P F+ ++++L + E R+ P D ++ + W KDG+ L A SR +T + G++ L I D G Y C A + G + +A + + ++ + +Q P +Q+I +L+ ++ Y EE++ P+FL L + +++GQ H E +E SD LV EW+ +G S+ +RI YD GY ALDI+ ED G Y VARN LG+A+ Q+ + + T+S++ SS+ + ++LEN +Y+ + P F+ P+ DP+ I +G + + ++P + +W+HNG V SR + AL+I+ +D+G Y V + H + V ++ + Q+ + + I LE + +ED I P F + L I+ + EG +V L +++P D S+ +EW+ N + G R + +Y+ L+++ + DSG Y A N+ G+ + SC +I+ + L+ ++ +G Q+++ LE+ ST + +E + P F P ++T EGG A F K+ P L EW
Sbjct: 890 PQFKTPIQNQLDLREHAHAHFEARLEPVGDSTMRVEWLKDGQPLEASSRITTYHNFGYVALTIKQLTIYDAGTYTCRAYNAMG-QDTTVAQLTVISKNEIVSESQHPG-GLQKIQHLE---DSSRYGRREEEETYITQAPRFLGPLKGTTKILEGQRAHFEARVEPQSDLGLVIEWYHNGRSITAANRIQTYYDFGYVALDISQVRAEDAGVYLVVARNKLGEAQQQATMI-------VETRSSIDTSSMHRGLYEKTQNLENKPFVEPQYDIEEISKSKPVFVTPLSDPKPI--HDGKNIHLECRLEPMGDPTMRVEWFHNGRPVTVGSRFRTYYDFGFVALDIIKATAADSGEY-TVRATNHLGTAHTSACVRVIDHTDVVTETQNEQSLEQIQLLEDSRR--RHHQEEDITIMQAPQFTRGLHNIETI-EGTNVHLECRLQPVGDPSMRIEWFVNGKPVKTGHRFRPAYEFDYVALDLLGCYAIDSGVYTCQARNQLGEAVT-----SC--SVRIIAKNDLILETQNESGLQKIQYLED-------STRHRRSEFVDEVVNIRPRFLTHPKSLTNTREGGHAHFECKIEPVTDPNLKVEW 1460 HSP 9 Score: 203.371 bits (516), Expect = 4.621e-52 Identity = 176/665 (26.47%), Postives = 298/665 (44.81%), Query Frame = 3
Query: 252 YDSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPE--EMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDP-QSKGISAIHALE------AELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADS-------AGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNI-TAGEGGPAKFLVKVSGFPTPTL--EWRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLTV 2189
Y P + + L++E + E +P+ L V P +D +L I W+ +GK+L+ GSRF + GF+ +D++ D G Y C A + +G A ++ + E+ ++ TQ P+ E +++I +L+ + G + ++ P+F ++ N + +G+ H E L D S+V EWF +G L+ R+ IY G AL++ ED G Y+C A N G AE SCN+ C ++ P + R+ S I+P++ + +G A+F+ T+ P N L +WYHNG++++ +R+ + L+I Y D+G Y+C ++++G + TLN + D Q + I LE A+ +AP ++ + P FI Q+ + +L EG S ++ P D L VEWY N + +G R + +D G L+I+ E+SG Y A NK G+ + + ++ C + SSL+ DS G +++ +LE + T + T+ P F V NI EG A F +++ P L EW + + S +T+ D LEI+ + A N G A AT+ +
Sbjct: 2889 YPPPVWTKPLQAEFHLSEAQPIHLEANVEPKEDPNLFIEWYFNGKMLNHGSRFKMTSEFGFVTMDMIEVYARDQGIYTCKAYNKAGEAFTSTTIFCSSKENIIES-TQHPKGAEGLEQIQDLEDSLRKD---GSKPEQPDLGIPPRFTTEFVNIADIGEGELAHFEANLIPVGDQSMVIEWFYNGKVLEASHRVRTIYAFGTVALEVLGTKIEDTGTYTCRATNKHGTAEI-SCNLEC----------------------VDKPRGQKPRFT---------SHIQPLE----GLKDGQSAHFECTLIPVNDPDLKVEWYHNGKLMRHSNRIKTVSDFGYVVLDISYLQDHDSGEYVCRAWNKYGE-DFTRTTLNCGGRGGVFYDSLQPDSLQRIRELECPQGQQADTSAP--------LVAEPPKFITQIVDVTKLVEGQSAHFEARLTPITDPDLVVEWYFNGKKLPHGHRFRTFHDFGIVILDILYCYEENSGVYEARARNKYGEDVT-RASLKC------ASKSSLILDSQLPRGMEGGLEKIANLEYSMVRTREETTEETKG----KAPVFTVPLENIENLREGENAHFEARITPADDPKLKVEWYWNGRPLKAGSRFRTFCDFGFVILEISPVYPEDSGEYSCRAINEYGEAVTTATMKI 3493 HSP 10 Score: 199.134 bits (505), Expect = 8.729e-51 Identity = 158/532 (29.70%), Postives = 248/532 (46.62%), Query Frame = 3
Query: 168 TMAPHALEVVNQVNKRSLASPRRGSDHLYDSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKA-ETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPID-PREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQ-SKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQS 1754
T P ALE + ++ + + D P F +L + EG+ +V P D +L I ++ +GK L + SRF FD G++ LDI + + +D G+Y A + G A S+ ++R+ P + TQ PE +++I L+ T + E + + P F L N + + Q H E L D +L EW+++ ++ SRI +D G+ +LDI+ ED G Y C A N LG+A T S V E+ I SIS++ LE P R + + P F + + P E+ EG A+F+ + P L +W+ NG ++ SRL + L+I +P D G Y+C ++ G S + V+T+ +I P + AI EA +N EM D P F L+ D+L+EG V L +VEP D +L +EW+KN + GSR++ ++D G TL+I ++DS Y ATN+ G+ S
Sbjct: 2191 TQHPDALEKIQKLESKVPNARTEVGDAPISPPHFTAELRGSTEIYEGQTAHFEAQVAPVHDPNLRIEFYHNGKPLPSASRFHITFDFGYVSLDITHAVAEDAGEYSVRAVNALGQAVSS-TNLRVIPRGTIISDTQHPE-GLEKIRKLES---TAPHQRQEPETPGTRQRPVFTQPLQNIDRINEHQTAHFEARLIPVGDPNLKVEWYRNEKIIEDSSRITKQHDFGFVSLDISHIRKEDEGVYMCRAVNPLGEAVTTASMRVVS---EASIQMDTQHPDSISRIHQLEKPL--APRPTEPERLFEKPIFTQLLTGPSELW--EGTHAHFEARVVPVGDPSLKFEWFINGVELQMGSRLRTTHDFGFVTLDITAVVPEDAGVYMCRAYNAAGEAVSS-TAMKVKTKSNIDGQPLIPESWEAIRLKEAAMNR-VPEMFVDSTPQQAPVFTTHLQSYDKLHEGQHVLLEAQVEPRADPNLRIEWFKNGISLTTGSRIRSTFDFGLVTLSINGLRADDSAIYTCKATNQVGEAVS 2708 HSP 11 Score: 196.823 bits (499), Expect = 5.593e-50 Identity = 171/634 (26.97%), Postives = 289/634 (45.58%), Query Frame = 3
Query: 168 TMAPHALEVVNQVNKRSLASPRRGSDHLYDSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEE----MMQE--INNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKA-ETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQ----SKGISAIHALEAEL-NAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNIT-AGEGGPAKF---LVKVSGFPTPTLEW 2021
T P ++ ++Q+ K LA + L++ P F Q L + EG RV P D SL+ WF +G L GSR T D GF+ LDI + +D G Y+C A + +G A S+ A +++ +S +D +PE ++E +N + + + T + P F + L + L +GQ + +E +E +D +L EWFK+G+SL GSRI +D G L I +D+ Y+C A N +G+A T S + + + +L S+ ++ LE P E R P FI ++ I E F+ ++P +S +W++NG+ ++ ++ + AL++ + ++G Y C + G + TL K++ LD Q G+ A+ E EL N ++ + E P + K L+ L+E + L VEP D +L++EWY N + +GSR +++ + G T++++ + D G Y A NK G+ + I C E I+ ++ + G ++++DLE+ L Q + P F + +NI GEG A F L+ V G + +EW
Sbjct: 2458 TQHPDSISRIHQLEK-PLAPRPTEPERLFEKPIFTQLLTGPSELWEGTHAHFEARVVPVGDPSLKFEWFINGVELQMGSRLRTTHDFGFVTLDITAVVPEDAGVYMCRAYNAAGEAVSSTA-MKVKTKSNIDGQPLIPESWEAIRLKEAAMNRVPEMFVDST----------PQQAPVFTTHLQSYDKLHEGQHVLLEAQVEPRADPNLRIEWFKNGISLTTGSRIRSTFDFGLVTLSINGLRADDSAIYTCKATNQVGEAVSTSSLKIE---DRHWLQAESLHPDSLPRIGELEAPKE--GRPEAPEPTYETPVFITHLN--NIECKESDNVRFECNVEPARDPTMSIEWFYNGQPLQAAAKFKSIYDFGYCALDLTNSYAENSGVYTCKATNSKGSATTSG-TLKCTGGKTMFLDTQHPQGEAGLEAVQETEEELANRYTSKTTKPETQYPPPVWTKPLQAEFHLSEAQPIHLEANVEPKEDPNLFIEWYFNGKMLNHGSRFKMTSEFGFVTMDMIEVYARDQGIYTCKAYNKAGEAFT-STTIFCSSKENIIESTQHPKGAEGLEQIQDLEDSLRKDGSKPEQPDLGIP----PRFTTEFVNIADIGEGELAHFEANLIPV-GDQSMVIEW 3065 HSP 12 Score: 194.126 bits (492), Expect = 3.669e-49 Identity = 142/516 (27.52%), Postives = 251/516 (48.64%), Query Frame = 3
Query: 243 DHLYDSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQE-INNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTES-----SISQLK-SLENPGEEYARYNDTSVIP---MPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQ----SKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQT 1748
D + P F ++++ L V EG P+ RV P D ++ I WF +G +++ GSR + D GFI LD+ Y D+G+Y C A + GTA ++ A V + + Y +QLPE M E + L++ +EE PKF +Q+ S+T+ + + + E +E +D SL EW+++G L G R I+D G+ +LDI + Y ED+G+Y C A N+ G+ T++ V+C +I+ ++ + +++Q++ +++ E D P PP F+ I +++++E A F+ + P + +W+ NG+ + +R + A+N + P D+G Y+C + +G+ E+ + + V + I+ D Q + I I +EA E+ D P F+ +LEP + EGD V+V V W N + +++GSR +++ D G L++ D+G +IA N G++
Sbjct: 3670 DEQPNPPKFTEEIKDNLDVPEGGPIHFDCRVEPVGDPTMRIEWFYNGHVMATGSRVHQLNDFGFIALDVDYIYARDSGEYTCRATNKWGTATTS-AKVTCKGKHNIVYESQLPEGMTSEKLKELERGRIPEAPKVVEE----VFGPPKFTTQI-TSVTVDEAEAVRFECQVEPKTDPSLRVEWYRNGKPLPSGHRYRNIFDMGFVSLDILYVYGEDSGEYVCRAINNYGEDRTRAT-VSCKKLPTILLQNQVPRGMKRSDALTQMEATIKKYTSEVHLTEDDLFDPDRKQPPRFVTQIK-EQLTLTEMAVTKFECQLAPVGDPNMKVEWFFNGKPLLHKNRFQPIYDFGYVAMNFGWVYPEDSGEYVCRATNLYGKDETRAI-IKVSGKPGIVYDSQLPAHMQSIDRIREMEASWQVVPDEVDPDAKPRTKPVFVSKLEP-QTVEEGDPARFCVRV--TGHPRPRVMWLINGHTVVHGSRYKLTND-GMFHLDVPKTRQYDTGKVEVIARNSVGES 4172 HSP 13 Score: 180.259 bits (456), Expect = 8.676e-45 Identity = 139/515 (26.99%), Postives = 239/515 (46.41%), Query Frame = 3
Query: 258 SPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYA-RYNDTSVIPM----PPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQ----SKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISC 1775
+P F L+S + EG+ + L +V P D +L I WFK+G L+ GSR + FD G + L I DD+ Y C A + G A S + ++I + + P+ + + I L+ E G E P F++ L N++ + + E +E D ++ EWF +G L+ ++ IYD GYCALD+ Y E++G Y+C A NS G A T S + C +++ + + + L++++ EE A RY + P PP + +P+ E +SE P + + ++P L +WY NG+++ SR E ++++ D G Y C +++ G + T+ ++++II Q ++G+ I LE L ++ ++ ++ P F + I + EG+ + P D S+ +EW+ N + R++ Y GT L ++ ED+G Y ATNK+G + ISC
Sbjct: 2621 APVFTTHLQSYDKLHEGQHVLLEAQVEPRADPNLRIEWFKNGISLTTGSRIRSTFDFGLVTLSINGLRADDSAIYTCKATNQVGEAVST-SSLKIEDRHWLQAESLHPDSLPR-IGELEAPKE-----GRPEAPEPTYETPVFITHL-NNIECKESDNVRFECNVEPARDPTMSIEWFYNGQPLQAAAKFKSIYDFGYCALDLTNSYAENSGVYTCKATNSKGSA-TTSGTLKCTGGKTMFLDTQHPQGE-AGLEAVQETEEELANRYTSKTTKPETQYPPPVWTKPLQ-AEFHLSEAQPIHLEANVEPKEDPNLFIEWYFNGKMLNHGSRFKMTSEFGFVTMDMIEVYARDQGIYTCKAYNKAGEAFTS-TTIFCSSKENIIESTQHPKGAEGLEQIQDLEDSLRKDGSKPEQPDLGIP-PRFTTEFVNIADIGEGELAHFEANLIPVGDQSMVIEWFYNGKVLEASHRVRTIYAFGTVALEVLGTKIEDTGTYTCRATNKHGTAE-----ISC 3117 HSP 14 Score: 155.221 bits (391), Expect = 6.602e-37 Identity = 120/399 (30.08%), Postives = 190/399 (47.62%), Query Frame = 3
Query: 144 EGVIPNGTTMAPHALEVVNQVNKRSLASPRRGSDHLYDSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEM-----MQEINNLDKNYETGTYHGMEED--KHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQS-CNVTCHPEESIITKSNLTE--SSISQLKSLENPGEEYARYNDTSVIP-MPPSFIEPIDPREISVSEGAPAYF--KVTIDP---------------GNGQKLSNDWYHNGEIVKTCSRLTGKLEM 1256
E +P G T E + ++ + + + + ++ P F + S + VDE E +R +V P D SL + W+++GK L +G R+ IFD GF+ LDILY +D+G+YVC A ++ G ++ A V + Q+P M + ++ K Y T H E+D K P+F++Q+ LTL + E L D ++ EWF +G L +R IYD GY A++ + YPED+G+Y C A N GK ET++ V+ P I+ S L SI +++ +E + D P P F+ ++P+ +V EG PA F +VT P G+ KL+ND + ++ KT TGK+E+
Sbjct: 3777 ESQLPEGMTS-----EKLKELERGRIPEAPKVVEEVFGPPKFTTQITS-VTVDEAEAVRFECQVEPKTDPSLRVEWYRNGKPLPSGHRYRNIFDMGFVSLDILYVYGEDSGEYVCRAINNYGEDRTR-ATVSCKKLPTILLQNQVPRGMKRSDALTQMEATIKKY-TSEVHLTEDDLFDPDRKQPPRFVTQIKEQLTLTEMAVTKFECQLAPVGDPNMKVEWFFNGKPLLHKNRFQPIYDFGYVAMNFGWVYPEDSGEYVCRATNLYGKDETRAIIKVSGKP--GIVYDSQLPAHMQSIDRIREMEASWQVVPDEVDPDAKPRTKPVFVSKLEPQ--TVEEGDPARFCVRVTGHPRPRVMWLINGHTVVHGSRYKLTNDGMFHLDVPKTRQYDTGKVEV 4163 HSP 15 Score: 143.665 bits (361), Expect = 2.756e-33 Identity = 170/678 (25.07%), Postives = 292/678 (43.07%), Query Frame = 3
Query: 258 SPTFQQDLESELVVDEGEPLRLMTR-VGPADDGSLEINWFKDG-KLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRIN-PESGVDYLTQ-----LPEEMMQ-------EINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSC-NVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIP----MPPSFIEPIDPREI-SVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILD----PQSKGISAIHALE-AELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAG--EGGPAKFLVKVS-----GFPTPTLEWRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVH-VAATNPLGVAKEEATLTV 2189
+P F Q S + V EGEP+ + +G I W KDG ++ S RF I D G L+I +D G Y C A++ +G+ +N A + + P +Y + P ++++ EI L ++ E H ++ P+F+ L N + +G +H+E +E D ++V EW+ +G L +R ++ G+ ALD+ D+G+Y C N+ G AE+++ +V P I +S+ +S+ + LE+ Y+RY T I P FI P+ R++ EG +F+ + P N + +WY +G + SR+T +LNIL+ D G+Y + G S+ ++ V + + D Q + I + LE + + ++E P F ++ L E ++EP D+++ VEW K+ + SR+ ++ G L I D+G Y A N GQ + ++ +IV+ S G Q+++ LE+ G + E+ P F + P+ T EG A F +V G +EW + + + +TY D L+I++ D V+ V A N LG A+++AT+ V
Sbjct: 470 APQFVQRF-STMTVREGEPITMSANAIGTPQP---RITWQKDGVQISSTAERFVGI-DGGATCLEIPRVTANDAGWYQCTAQNIAGST-ANRARLYVEVPREQPNYEQRRLNLPRPTKVIEPEPIPGPEIIYL-RHVERAKPHLRPGEEDRVYPPPQFIIPLQN-VQQTEGGRVHMEARIEPVGDPTMVVEWYLNGRPLAASARATSVFKFGFIALDLLSIMGHDSGEYMCRVTNASGVAESRAILSVVQRPS---IEQSSQNPNSLQYINQLED----YSRYQRTESIDEQLNQAPQFIRPL--RDLGEFEEGKNVHFEAQVTPVNDPSMRVEWYKDGLPITASSRITAIFNFGYVSLNILHLRAEDAGTYTVRAVNRIGEAISQ-SSIRVHSRSQVTADLGIPEQQRYIEKVEELEDYRKSQQRRHVQEAAEAIAPPQFKTPIQNQLDLREHAHAHFEARLEPVGDSTMRVEWLKDGQPLEASSRITTYHNFGYVALTIKQLTIYDAGTYTCRAYNAMGQDTT-VAQLTVISKNEIVSESQ---HPGGLQKIQHLEDSSRYGR----REEEETYITQAPRF-LGPLKGTTKILEGQRAHFEARVEPQSDLGL---VIEWYHNGRSITAANRIQTYYDFGYVALDISQVR-AEDAGVYLVVARNKLGEAQQQATMIV 1116 HSP 16 Score: 117.472 bits (293), Expect = 3.339e-25 Identity = 135/534 (25.28%), Postives = 222/534 (41.57%), Query Frame = 3
Query: 678 PKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEES---------------IITKSNLTESSISQLKSLEN------PGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQS-KGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQE--ECL--PNFDVKPMN-ITAGEGGPAKFLVKVS--GFPTPTLEWRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLTVI 2192
P+F+ + +++T+ +G+P+ + + W KDG+ + + V D G L+I D G Y C A+N G ++ P E +I + I L+ +E PGEE Y PP FI P+ + + +EG + + I+P + +WY NG + +R T + AL++L + D+G Y+C + + G ES + L+V SI Q+ + I+ LE + E DE + P FI+ L + + EG +V +V P ND S+ VEWYK+ I SR+ ++ G +LNI+ +ED+G Y + A N+ G E I+ S ++ + S + AD ++ + +E+ + Q VQE E + P F N + E A F ++ G T +EW D + S TY++ L I + + A N +G A LTVI
Sbjct: 471 PQFVQRF-STMTVREGEPITMSANAIGTPQPRIT--WQKDGVQISSTAERFVGIDGGATCLEIPRVTANDAGWYQCTAQNIAGSTANRARLYVEVPREQPNYEQRRLNLPRPTKVIEPEPIPGPEIIYLRHVERAKPHLRPGEEDRVYP-------PPQFIIPL--QNVQQTEGGRVHMEARIEPVGDPTMVVEWYLNGRPLAASARATSVFKFGFIALDLLSIMGHDSGEYMCRVTNASGVAESRAI-LSVVQRPSIEQSSQNPNSLQYINQLEDYSRYQRTESI-DEQLNQAPQFIRPLRDLGEFEEGKNVHFEAQVTPVNDPSMRVEWYKDGLPITASSRITAIFNFGYVSLNILHLRAEDAGTYTVRAVNRIG----EAISQSSI---RVHSRSQVTADLGIPEQQRYIEKVEELEDYRKSQQRRHVQEAAEAIAPPQFKTPIQNQLDLREHAHAHFEARLEPVGDSTMRVEWLKDGQPLEASSRITTYHNFGYVALTIKQLTIYDAGTYTCRAYNAMGQDTTVAQLTVI 983 HSP 17 Score: 78.5666 bits (192), Expect = 2.600e-13 Identity = 105/387 (27.13%), Postives = 161/387 (41.60%), Query Frame = 3
Query: 1083 PPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQL-EPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYD-RGTATLNIVSAISEDSGDYWLIATNKNGQTQSE-KINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVK-PMNITAGEGGPAKFLVKVSGFPTPTLEWRIDD--VLVVPDSVCKTYNDGSINYLEINRC--SELGDIKVHVAATNPLGVAKEEATLTVIPMV---DFR 2210
PP FI+P++P+ E A V +P + S W+ + E +K+ + + + N S L I G + C +E V +V + ++ L PQ + E +P+ F+++L +P++ ++ G+++ LT +V + VEWY N I IS D +G L I E+ G Y +ATNK G++ S+ + I FE + DS L EEDL +S +E P K P I EG AK VKV G P P ++W DD + + + + DG+ + L IN +LG I A NPLGVA A V +V D+R
Sbjct: 6840 PPRFIQPLEPKYFGEHEVAIIEAIVESEPLS----SFQWFVHNEPIKSSNEVRIVSQANKSTLLIENFQSKFVGPFTC---------RAENVGGSVTSTATVNLIPQEEA--------EEFESPR--------------FVEELVQPVEVMD-GEALLLTCQV--TGKPTPKVEWYHNAEKITENKETTISQDLQGVCQLQITEVFPENEGQYECVATNKIGKSVSKTNVKIQAFE---------YIPDSE-ITGLTGSEEDLLDRTLSI--------DEQAPKIIKKLPEKIEPKEGEQAKLEVKVVGKPKPKVKWLRDDEQIFASEEYQIENFEDGT-SVLVINHVYPDDLGTISFE--AYNPLGVAVTTALFAVEGIVGSKDYR 7167 HSP 18 Score: 62.3882 bits (150), Expect = 2.454e-8 Identity = 101/484 (20.87%), Postives = 174/484 (35.95%), Query Frame = 3
Query: 363 INWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAK---TIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPK--AEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQS------EKINISCFE 1781
+ W+++ + G +L I +D+ + CVA++ GTA+ + V +EE + A K P F++ + S T+ GQ + + L W K+GM ++ + ++ + L I + ED+G Y CVA N+ G+A C+ C I+ S S+ + PG E A P +E + + +V EG+ F+ +D G + W VK + L I P D G+Y CV ++ G ++QT + + P L+AP +K+ + +P+ K T+T KV + V+W++ I Q+ +D +A L I + EDSG + + T+ GQ +S +K IS F+
Sbjct: 4425 VKWYRESFPIQNSPDLQIHTFSGKSILIIRQVFVEDSAVFSCVAENRGGTAKCSANLV------------------------------------VEERRRAGKGGIQPPSFVTTI-QSTTVATGQLARFDAKV--TGTRPLDVYWLKNGMKIQPSIKFKMLEEDSVHTLLIIEPFAEDSGRYECVAVNAAGEA---RCDGDC-----IV-------QSPSKPEKPTTPGSEKA-----------PHIVEQLKSQ--TVEEGSKVIFRCRVD-GKPTPTAR-WMRGENFVKPSRYFQMSRQGEYYQLVISEAFPEDEGTYKCVAENKLG---------SIQTSAQLKVRPIEN-----------LDAPPTITALKDVSVTEGMPAQFK-------------TTVTGKV-----KATSVQWFREGQLIPETPDFQMIFDGNSAVLLIGTTYEEDSGIFTVRVTSSTGQVESSAKLTVKKRRISAFQ 4801 HSP 19 Score: 57.3806 bits (137), Expect = 8.496e-7 Identity = 98/443 (22.12%), Postives = 165/443 (37.25%), Query Frame = 3
Query: 738 IEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEP-IDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMN-ISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEK-SIILDPQSKGISAI------HALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQI-SYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEWRIDDV 2036
IE +E SS +WF +K + + ++ L I + G ++C A N +G + T + V P+E EE+ P F+E + P E+ E +VT P +WYHN E + T ++ + L I P + G Y CV ++ G+ S+ NV+ + I D + G++ L + APK II LP +++EP EG+ L VKV V+W +++ I QI +++ GT+ L I +D G A N G + + F E IV S ++++++ L + C P+ + + A G AKF ++ +G P P ++W ++V
Sbjct: 6860 IEAIVESEPLSSF--QWFVHNEPIKSSNEVRIVSQANKSTLLIENFQSKFVGPFTCRAEN-VGGSVTSTATVNLIPQEE---------------------AEEFES----------PRFVEELVQPVEVMDGEALLLTCQVTGKPTP----KVEWYHNAEKITENKETTISQDLQGVCQLQITEVFPENEGQYECVATNKIGKSVSK---TNVKIQAFEYIPDSEITGLTGSEEDLLDRTLSIDEQAPK-------IIKKLP---EKIEP----KEGEQAKLEVKV--VGKPKPKVKWLRDDEQIFASEEYQIENFEDGTSVLVINHVYPDDLGTISFEAYNPLGVA----VTTALFAVEGIV-GSKDYRKPEWVSQMEEMQVALKAAK-------------CSPSLLNEMRDCRAALGETAKFSIQFAGNPIPDIQWYFNNV 7227 HSP 20 Score: 55.0694 bits (131), Expect = 3.879e-6 Identity = 77/371 (20.75%), Postives = 150/371 (40.43%), Query Frame = 3
Query: 252 YDSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFD-RGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRIN-----PESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVI-YDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESII-TKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSN-DWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHG 1337
++SP F ++L + V +GE L L +V + ++ W+ + + ++ + D +G L I ++ G Y CVA + G + S +V+I P+S + LT E+++ ++D+ PK + +LP + +G+ +E+ + + +W +D + + ++ G L I YP+D G S A N LG A T + E I+ +K +SQ++ ++ + A+ + PS + + R+ + G A F + G + + WY N ++ + ++ + L I+ D G Y C L +E G
Sbjct: 6939 FESPRFVEELVQPVEVMDGEALLLTCQV--TGKPTPKVEWYHNAEKITENKETTISQDLQGVCQLQITEVFPENEGQYECVATNKIGKSVSK-TNVKIQAFEYIPDSEITGLTGSEEDLLDRTLSIDEQ------------------APKIIKKLPEKIEPKEGEQAKLEVKVVGKPKPKV--KWLRDDEQIFASEEYQIENFEDGTSVLVINHVYPDDLGTISFEAYNPLGVAVTTALFAV----EGIVGSKDYRKPEWVSQMEEMQV-ALKAAKCS--------PSLLNEM--RDCRAALGETAKFSIQF---AGNPIPDIQWYFNNVQLRASEKYRMVVQEQEATLEIMKITSEDCGYYNCKLINEIG 7268
BLAST of Titin vs. UniProt/SwissProt
Match: sp|O01761|UNC89_CAEEL (Muscle M-line assembly protein unc-89 OS=Caenorhabditis elegans OX=6239 GN=unc-89 PE=1 SV=3) HSP 1 Score: 112.849 bits (281), Expect = 9.215e-24 Identity = 157/621 (25.28%), Postives = 245/621 (39.45%), Query Frame = 3
Query: 354 SLEINWFKDGKLL--SAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDR-GYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFK--VTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNIS-ALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYD--RGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLVVP-DSVCKTYNDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLTV 2189
+ E+ WFKDGK + +A + D G + I+ E D G Y+CVA + G A++ + V+ L + EE +E +A K P + N GQP+ + ++ +S+V W+KDG+ L+ SR + Y+ G L I ED G Y CVA N+ G T SC+V + + K GEE P F + + ++ G K VT DP K WY NG++++ R + + S +L + + SD G Y C + HG+ +++ T +VQ A+ E K +M E + P FI +L + ++ G+ + L KV + S V+W K+ +I SR + S + +G L I +A D G Y +ATN+NG SA + +++ L SG ++ Q P F +K ++ EG P K KV P P + W D +V P D + + + + L I C D V ATNP G A+++ T TV
Sbjct: 5305 TAEVEWFKDGKKIEHTAHTEIRNTVD-GACRIKIIPFEESDIGVYMCVAVNELGQAETQATY-------QVEILEHVEEEKRRE--------------------YAPKINPPLEDKTVNG-----GQPIRLSCKVDAIPRASVV--WYKDGLPLRADSRTSIQYEEDGTATLAINDSTEEDIGAYRCVATNAHGTINT-SCSVNVKVPKQEVKKE----------------GEE-------------PFFTKGL--VDLWADRGDSFTLKCAVTGDPFPEIK----WYRNGQLLRNGPRTVIETSPDGSCSLTVNESTMSDEGIYRCEAENAHGKAKTQ-ATAHVQM--------------ALGKTE------KPKMDEGK----PPKFILELSDM-SVSLGNVIDLECKVTGLPNPS--VKWSKDGGPLIEDSRFEWSNEASKGVYQLRIKNATVHDEGTYRCVATNENG--------------------------SATTKSFVRMDDGLGSGVVTASQP---------PRFTLKMGDVRTTEGQPLKLECKVDASPLPEMVWYKDGAIVTPSDRIQISLSPDGVATLLIPSCVYDDDGIYRVIATNPSGTAQDKGTATV 5791 HSP 2 Score: 95.1301 bits (235), Expect = 2.138e-18 Identity = 164/676 (24.26%), Postives = 257/676 (38.02%), Query Frame = 3
Query: 303 EGEPLRLMTRVGPADDGSL-EINWFKDGKLLSAGSRFS-TIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYD-RGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNG------EIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHII----NGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEE----CLPNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVHVA-ATNPLGVAKEEATLTV-IPMVD---FRPDLR---HITPENPFKKM 2255
EG+PL+L +V D L E+ W+KDG +++ R ++ G L I + DD+G Y +A + SGTAQ + G + +LP + +G + D A PK + L N + + + Q + + D +WFKDG + R+ +I G C L + +D G Y CVA N+ G A T SC+V + K +SSI + K+ P F P+ R G F+ P S W +G E +K + G + +S + L G + CV +EHG ++ L ++ +++I P E+N E K P + L + ++EG+ V + V + V+WYK+ I+ +G R+ + +RG L IV+A +D G+Y L ATNK G ++E S NI ++ D E P F + N P F V G P P +EW + +VP K + G ++ I + L D +VA A N G A A L V +P +D F ++ ++T E FKK+
Sbjct: 5714 EGQPLKLECKV---DASPLPEMVWYKDGAIVTPSDRIQISLSPDGVATLLIPSCVYDDDGIYRVIATNPSGTAQ----------DKGTATVKKLPRD-------------SGARRSADRDVFDANKAPKLMEPLEN-IRIPEKQSFRLR--CKFSGDPKPTIKWFKDGERVFPYGRLQLIESPDGVCELVVDSATRQDAGGYRCVAENTYGSART-SCDVNVIRGDR---KPRDIDSSIREGKA--------------------PGFTTPLTIRR--AKPGDSVTFECL--PFGNPFPSIKWLKDGLELFSDEKIKMEAAADGTQRLILSDVTFL-----SEGYFRCVATNEHGTASTK-AELVIEGDRTIGSRPL-----------PEVNGEPEECK--------PRIRRGLYNM-SIHEGNVVEMIVCA--TGIPTPTVKWYKDGQEIVGDGPDGKRVIFTDERGIHHLVIVNASPDDEGEYSLEATNKLGSAKTE-----------------------------------GSLNIIRPRHIADADERGGMPFPPGFVRQLKNKHVFNHMPTIFDCLVVGHPAPEVEWFHNGKKIVPGGRIKIQSCGGGSHALIILDTTLEDAGEYVATAKNSHGSASSSAVLDVTVPFLDSIKFNGEIDVTPYLTEEYGFKKL 6269 HSP 3 Score: 77.7962 bits (190), Expect = 4.403e-13 Identity = 112/486 (23.05%), Postives = 193/486 (39.71%), Query Frame = 3
Query: 729 PLHIEIFLEHHSDS--SLVTEWFKDGMSLKLGSRIDVIYD-RGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISA-LNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQI-SYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLVVPDSVCKTYNDGS--INYLEINRCSELGDIKVHVAATNPLGVA 2165
P+ +F+E H D+ + EWFKDG ++ + ++ G C + I D G Y CVA N LG+AETQ+ Q++ LE+ EE R + P P++ + +V+ G P +D + S WY +G ++ SR + + E + +A L I + D G+Y CV + HG + + ++NV+ PK E+K++ + P F K L + + GDS TL V D ++WY+N + NG R I + G+ +L + + D G Y A N +G+ +++ A++ + + G ++E P F ++ +++ G KV+G P P+++W D ++ DS + N+ S + L I + + ATN G A
Sbjct: 5289 PVGDPMFIECHVDANPTAEVEWFKDGKKIEHTAHTEIRNTVDGACRIKIIPFEESDIGVYMCVAVNELGQAETQAT---------------------YQVEILEHVEEEKRREYAPKINP-------PLEDK--TVNGGQPIRLSCKVD--AIPRASVVWYKDGLPLRADSRTSIQYEEDGTATLAINDSTEEDIGAYRCVATNAHGTINTS-CSVNVK-------------------------VPKQEVKKE---GEEPFFTKGLVDL-WADRGDSFTLKCAV--TGDPFPEIKWYRNGQLLRNGPRTVIETSPDGSCSLTVNESTMSDEGIYRCEAENAHGKAKTQ--------------ATAHVQMALGKTEKPKMDEGKP------------------PKFILELSDMSVSLGNVIDLECKVTGLPNPSVKWSKDGGPLIEDSRFEWSNEASKGVYQLRIKNATVHDEGTYRCVATNENGSA 5678 HSP 4 Score: 74.3294 bits (181), Expect = 4.780e-12 Identity = 143/689 (20.75%), Postives = 258/689 (37.45%), Query Frame = 3
Query: 171 MAPHALEVVNQVNKRSLASPRRGSDHLYDSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDR---GFIVLDILYTIEDDNGDYVCVAKSHSGTAQSN-IAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYD--RGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYND---TSVI--PMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMN-ISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQ-ISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNI-TAGEGGPAKFLVKVSGFPTPTLEWRIDDVLVVPDSVCK--TYNDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLTV 2189
+A +A +N ++ P++ + P F + L +L D G+ L V D EI W+++G+LL G R T+ + G L + + D G Y C A++ G A++ AHV++ T+ P+ M+E K PKF+ +L + +++ G + +E + + S+ +W KDG L SR + + +G L I D G Y CVA N G A T+S + R +D + V+ PP F + ++ +EG P + +D ++ WY +G IV R+ L + ++ L I + D G Y + + G Q KG + + L + A ++ D + D K +EP++ + + + ++ + + D ++W+K+ + R+Q I G L + SA +D+G Y +A N G ++ +++ G++ + +D++ + G F P+ I A G F G P P+++W D + + D K DG+ L ++ + L + ATN G A +A L +
Sbjct: 5457 VATNAHGTINTSCSVNVKVPKQEVKKEGEEPFFTKGL-VDLWADRGDSFTLKCAV--TGDPFPEIKWYRNGQLLRNGPR--TVIETSPDGSCSLTVNESTMSDEGIYRCEAENAHGKAKTQATAHVQMALGK-----TEKPK--------------------MDEGKP-----PKFILEL-SDMSVSLGNVIDLECKVTGLPNPSV--KWSKDGGPLIEDSRFEWSNEASKGVYQLRIKNATVHDEGTYRCVATNENGSATTKS----------------------------------FVRMDDGLGSGVVTASQPPRFT--LKMGDVRTTEGQPLKLECKVDASPLPEMV--WYKDGAIVTPSDRIQISLSPDGVATLLIPSCVYDDDGIYRVIATNPSGTA-------------------QDKGTATVKKLPRDSGARRSA---DRDVFDANKAPKLMEPLENIRIPEKQSFRLRCKFSGDPKPTIKWFKDGERVFPYGRLQLIESPDGVCELVVDSATRQDAGGYRCVAENTYGSARTS-CDVNVIRGDR---------------KPRDIDSSIREGKAPGFTT---------------PLTIRRAKPGDSVTFECLPFGNPFPSIKWLKDGLELFSDEKIKMEAAADGT-QRLILSDVTFLSEGYFRCVATNEHGTASTKAELVI 6015 HSP 5 Score: 73.1738 bits (178), Expect = 1.264e-11 Identity = 146/658 (22.19%), Postives = 255/658 (38.75%), Query Frame = 3
Query: 255 DSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSR--FSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDV-IYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQS---CNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNIS-ALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQI-SYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLVVPDSVC--KTYNDGSINY-LEINRCSELGDIKVHVAATNPLGVAKEEATLTVIP 2195
++P F Q L+ V++G+P L+ RV E+ WFKDG ++ ++ + G L I T D G Y C A + +G +E + E+ ++E T A + P F+ L + ++G + +E + S + ++FK+ +++G + + + + G L I ED G Y C A N GKA T + EE + +S E ++ E G+ + DT P F+E + R +V+E A K + K+ W G+ V+ +R+ + + + + L +D G Y C +E+G + TE II+ LE APK I + P F++ ++P + G++ L K+ S V+WYKN + R++I + D GT L + +A +D +Y A+N+ G S+ + ++ E ++ P F + I E AKF KVSG P ++W D + D ++ +DG+ +E ++ + G+ ++ V +N GVA + LTV+P
Sbjct: 3578 EAPLFVQGLKP-YEVEQGKPAELVVRV--EGKPEPEVKWFKDGVPIAIDNQHVIEKKGENGSHTLVIKDTNNADFGKYTCQATNKAGK-----------------------DETVGELKIPKYSFEKQT---------AEEVKPLFIEPLKETFA-VEGDTVVLECKVNKESHPQI--KFFKNDQPVEIGQHMQLEVLEDGNIKLTIQNAKKEDVGAYRCEAVNVAGKANTNADLKIQFAAKVEEHVTDESGQLE----EIGQFETVGDTASSKTDTGR--GAPEFVELL--RSCTVTEKQQAILKCKVKGEPRPKIK--WTKEGKEVEMSARVRAEHKDDGTLTLTFDNVTQADAGEYRCEAENEYG---------SAWTEGPIIV-----------TLEG---APK-------IDGEAPDFLQPVKPA-VVTVGETAVLEGKISGKPKPS--VKWYKNGEELKPSDRVKIENLDDGTQRLTVTNAKLDDMDEYRCEASNEFGDVWSD-VTLTVKEPAQVA-----------------------------------------PGFFKELSAIQVKETETAKFECKVSGT-KPDVKWFKDGTPLKEDKRVHFESTDDGTQRLVIEDSKTDDQGNYRIEV--SNDAGVANSKVPLTVVP 4109 HSP 6 Score: 60.4622 bits (145), Expect = 9.391e-8 Identity = 151/653 (23.12%), Postives = 231/653 (35.38%), Query Frame = 3
Query: 255 DSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTI-FDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDV-IYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNIS--ALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQ-LNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSE-KINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLVVPDSVCKTYNDGSINYLEI--NRCSELGDIKVHVAATNPLGVAKEEATLTV 2189
++P F Q ++ VV GE L ++ S + W+K+G+ L R D G L + DD +Y C A + G S++ LT K A+ P F +L + IQ + F S + +WFKDG LK R+ D G L I +D G+Y N G A ++ +T P E++ K LT +++V++G V ++ G+ + WY E V T S+ T +++ S L I SDTG+Y VL ++ VES S ++ A E LPSF K L DQ + +G + L V++E + V+WYKN + I +G + G L I +D G+Y + A N+ G+ +S+ K+N+S + ++ SG + T T +G A F VKV G P ++W + +PD+ K DGS + LEI + + D KV V +N G A A LTV
Sbjct: 3918 EAPDFLQPVKP-AVVTVGETAVLEGKISGKPKPS--VKWYKNGEELKPSDRVKIENLDDGTQRLTVTNAKLDDMDEYRCEASNEFGDVWSDVT------------LTV---------------------------KEPAQVAPGFFKEL----SAIQVKETETAKFECKVSGTKPDVKWFKDGTPLKEDKRVHFESTDDGTQRLVIEDSKTDDQGNYRIEVSNDAGVANSK-VPLTVVPSETLKIKKGLT---------------------------------------DVNVTQGTKILLSVEVE---GKPKTVKWYKGTETV-TSSQTTKIVQVTESEYKLEIESAEMSDTGAYRVVLSTDSFSVES------------------SATVTVTKAAEK---------------ISLPSFKKGL--ADQSVPKGTPLVLEVEIEGKPKD---VKWYKNGDEIKDGKVEDLG--NGKYRLTIPDFQEKDVGEYSVTAANEAGEIESKAKVNVSA-------------------------KPEIVSGLVPT----------------------TVKQGETATFNVKVKG-PVKGVKW-YKNGKEIPDAKTKDNGDGSYS-LEIPNAQVEDAADYKVVV--SNDAGDADSSAALTV 4388
BLAST of Titin vs. UniProt/SwissProt
Match: sp|A2ASS6|TITIN_MOUSE (Titin OS=Mus musculus OX=10090 GN=Ttn PE=1 SV=1) HSP 1 Score: 109.768 bits (273), Expect = 7.364e-23 Identity = 177/772 (22.93%), Postives = 302/772 (39.12%), Query Frame = 3
Query: 360 EINWFKDGKLLSAGSRFSTIF-DRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPE-EMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLS---------------------QL-----------PNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSL--KLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIV--KTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPI------DQLNEGD-----------------SVTLT--------VKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLAD-SAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLTVIPMVDFRPDLRHI---TPE----NPFKKMAGLKKVEC---TSELNDAFKKSKPTAERIVKIEMGSET---KSKFYKSPDVIETEKLYDQVAA 2426
+I W+KDGK + G R+ F G L I + +D G Y A + G A + + + P + T +P E + I ++ + + M A+ P +S QL P S ++GQ ++ + T WF +G + ++ VI + G +L I P D+G+++ VA+N GK S I+ + E+ Q+K P+F+E + + +++ EG+ KV + W N +I+ R+ + +AL I + D+ Y ++ GR ++ +NV+ E +P+ E +L P+ + EI +LEP+ +Q EGD S+ L ++ P D ++ VEW + + +R+++ + G +L+ +A S DSG ATNK G + I + EK + S L D G QR+++LE G ++ T D +E+ P+ + P + EG A+F +V+G+P P + W ++ L+ + DG I+YL+I C +V V A NP GV + + L + DFR LR PE P K ++KV+ TSE + K + AERI ++ E+ +SKF + + E Y+ + A
Sbjct: 1323 KIAWYKDGKRIRHGERYQMDFLQDGRASLRIPVVLPEDEGIYTAFASNIKGNAICS-GKLYVEPAAPFSAPTYMPTPEAVSRIRSVSPRSLSRSPIRMSPAMSPARMSPARMSPARMSPARMSPGRRLEETDESQLERLYKPVFVLKPASFKCLEGQTARFDLKVVGRPMPE--TFWFHNGQQIVNDYTHKV-VIKEDGTQSLIIVPASPSDSGEWTVVAQNRAGK--------------STISVTLTVEAVEHQIK---------------------PAFVEKL--KNVNIKEGSRLEMKVRATGNPNPDIV--WLKNSDIIVPHKYPRIRIEGTRGEAALKIDSIISQDSAWYTATAINKAGR-DTTRCKVNVEVE---FAEPEP---------ERKLIIPRGTYRAKEIAAP------ELEPLHLRYGQEQWEEGDLYDKEKQQKPFFKKKLTSLRLKRFGPAHFECRLTPIGDPTMVVEWLHDGKPLEAANRLRMINEFGYCSLDYGAAYSRDSGVITCRATNKYGTDHTSATLI--VKDEKSLVEESQLPDGKKGLQRIEELERMAHEGALTGV--TTDQKEKQKPDIVLFPEPVRVLEGETARFRCRVTGYPQPKVNWYLNGQLIRKSKRFRVRYDG-IHYLDIVDCKSYDTGEVKVTAENPEGVTEHKVKLEIQQREDFRSVLRRAPEPKPEFHVHEPGKLQFEVQKVDRPVDTSETKEVVKLKR--AERITHEKVSEESEELRSKFKRRTE----EGYYEAITA 2021 HSP 2 Score: 78.9518 bits (193), Expect = 2.088e-13 Identity = 146/693 (21.07%), Postives = 269/693 (38.82%), Query Frame = 3
Query: 357 LEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTA-----------------------QSNIAHVRINPES--------GVDYLTQLPEE----MMQEINNL----DKNYETGTYHGMEEDKHAAKTI-------PKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSII-------LDPQSKGISAIHALEAELNAPKAEMKED------EIITDLPS---------------FIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNF--DVKPMNITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLVVPDSVCKTY---NDGSINYLEINRCSELGDIKVHVA-ATNPLGVAKEEATLTVIP 2195
+ + W KDG+ L R ++ L I T D G Y VA + TA Q + +N + G++ Q+ E + + I+ + ++ + G Y + ++ T+ P+ L +L +T+ G+P + + W+K+ L G + ++D L + +PED Y+C A+N G A T + PE + +P +E Y PP+ + P+ ++ SEG PA F+ + +G L WY + +K + L I P D G+Y V + G+V S TL ++ +SI+ ++ + K I+++ + E + ++++ E++++ P+ FI+++ + +++ D L+V V ++W+ N + + + +D T +L I+ +D G+Y +A+N+ G K V ++ L G +R ++E SG E + C P F ++KP++ G G PA F V G P PT+ W +D+ + SVC T DGS ++ +N + GD +++ A N G + A L V+P
Sbjct: 3092 ITVQWMKDGQELQIADRIKIQKEKYVHRLLIPSTRMSDAGKYTVVAGGNMSTANLFVEGRDVRIRSIKKEVQVIEKQRAVVEFEVNEDDVDAHWYKDGIEINFQVQERHQYVVERRIHRMFISETRHSDAGEYTFVAGRNRSSVTLYVNAPEPPQVLQEL-QPVTVQSGKPARFCAVISGRPQPKI--SWYKEEQLLSTGFKCKFLHDGQEYTLLLIEAFPEDAAVYTCEAKNDYGVATTSASLSVEVPE-------------------VVSPDQEMPVY--------PPAIVTPL--QDTVTSEGRPARFQCQV---SGTDLKVSWYCKDKKIKPSRFFRMTQFEDTYQLEIAEAYPEDEGTYAFVANNAVGQVSST-ATLRLEAPESILHERIGQQIEMEMKEIASLLSAEEDFQTYSSDLRLPNANETLELLSEPPARSTQFDSRQEGAAPVFIREISDV-EISVEDVAKLSVTVTGCPKPK--IQWFFNGMLLTPSADYKFVFDGDTHSLIILFTRFQDEGEYTCLASNEYG---------------KAVCSAHLRISPRG-ERSTEME----SGEKKAL---EKPKGPCPPYFFKELKPVH--CGPGIPAVFEYSVHGEPAPTVLWFKEDMPLY-TSVCYTIIHSPDGSGTFI-VND-PQRGDSGLYLCKAQNLWGESTCAAELLVLP 3717 HSP 3 Score: 77.0258 bits (188), Expect = 8.935e-13 Identity = 78/355 (21.97%), Postives = 130/355 (36.62%), Query Frame = 3
Query: 678 PKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNG 1742
P + + P +T+ G P +E + + L +WFKDG L GSR + + R +L I D G Y+ N +GK+ SC V+ H + ++ PPSF+ + ++ S + GA + + +S W+ +G + + + N+ L + PSDTG+Y CV + G+ ES V L VQ + PSF + + ++ L G S+T T + V+W+K +++G IS + L ++ SGDY + TN G
Sbjct: 7532 PATIVEKPEPMTVTTGNPFTLECVVAGTPE--LSAKWFKDGRELSSGSRHHITFVRNLASLKIPSAEMNDKGLYTFEVENRVGKS---SCTVSVHVSDRVV----------------------------------PPSFVRRL--KDTSATLGASVVLECRV--SGSAPISVGWFLDGNEIISSPKCQSSFADNVCTLTLSSLEPSDTGAYTCVAANVAGQDESSAV-LTVQ--------------------------------------EPPSFEQTPDSVEVL-PGMSLTFTSVIR--GTPPFKVKWFKGSRELVSGEACTISLEDFVTELELLEVEPGQSGDYSCLVTNDAG 7801 HSP 4 Score: 72.0182 bits (175), Expect = 2.953e-11 Identity = 118/561 (21.03%), Postives = 202/561 (36.01%), Query Frame = 3
Query: 180 HALEVVN-QVNKRSL----ASPRRGSD------HLYDSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAG 1829
H+LE+ N V R + AS + G+D + D P F ++LE+ V ++ +D + I W KDG+ L AG + F+ L+I D+G Y C A + +G++ S+ A P P F+ ++ S ++ G+ + L+ + WFK+ L + + + + LDI +D+G YSC A N +G + SC+ VI PPSFI+ ++P +I GA A + I G G +W+ + + +++ + + L I +D G Y CV+ +E G+ G A H L+ + P+F+K+++ L G +VTL V + V W K + I ++++S+ G A L I G Y +A N+ G +Q+ + E KI+ + L+ +AG
Sbjct: 4494 HSLELSNLTVQDRGIYSCKASNKFGADICQAELTIIDKPHFIKELEA---VQSAINKKIHLECQVDEDRKVTITWSKDGQKLPAGKDYKIYFEDKIASLEIPLAKLKDSGTYTCTASNEAGSSSSSAAVAVREP-------------------------------------------PSFVKKVDPSYLMLPGESARLHCKLK--GSPVIQVTWFKNNKELSESNTVRMSFVNSEAILDITDVKVDDSGTYSCEATNDVG---SDSCSTEV-------------------------------------VIKEPPSFIKTLEPADI--VRGANALLQCEIA-GTGP-FEVNWFKDKKQIRSSKKYRLFTQKTFVYLEISSFNSADVGDYECVVANEVGKC----------------------GCVATHLLK-----------------EPPTFVKKVDDFTALA-GQTVTLQAAVR--GSEPISVMWMKGQEVIKEDGKIKMSFSNGVAVLTIPDVQISLGGKYTCLAENEAG-SQTSVGELIVKEPAKIIERAELIQVTAG 4919 HSP 5 Score: 71.633 bits (174), Expect = 3.617e-11 Identity = 101/514 (19.65%), Postives = 178/514 (34.63%), Query Frame = 3
Query: 357 LEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPE---------------------------EMMQEINNLDKNYE---------------TGTY-------HGMEEDKHAAKTI-PKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVE-SEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQ 1745
+++W KD + L +G ++ + + + IL D G+Y C A + G+ + P V LT + E+++E +N+ +Y G Y GM+E + P + + P S+ + G +E + + L T+WFKDG L ++ + + L I P D+G YS +N +GK SC V+ + II PPSF + +E + G+ + + +S W+H+G + + + L N AL + +D G Y C+ + G E S P+T + + PSF+++ +P+D L G +VT T V+ V W+K ++ G+R +S L + + SG+Y I +N+ G+
Sbjct: 8316 FQVSWHKDKRELRSGKKYKIMSENLLTSIHILNVDTADIGEYQCKATNDVGSDTCVGSVTMKAPPQFVKKLTDISTIIGKEVQLQTTIEGAEPISVAWFKDKGEIVRESDNIWISYSENIATLQFSRAEPANAGKYTCQIKNDAGMQECYATLSVLEPAAIVEKPESIKVTTGDTCTLECTVSGTPE--LSTKWFKDGKELTSDNKYKISFFNKVSGLKIINVVPGDSGVYSFEVQNPVGK---DSCKVSIQVSDRII----------------------------------PPSFTRKL--KETNGLSGSSVVMECKV--YGSPPISVLWFHDGNEISSGRKYQTTLTDNTCALTVNMLEEADAGDYTCIATNVAGSDECSAPLT----------------------------------------VREPPSFVQKPDPMDVLT-GSNVTFTSIVK--GSPPFTVSWFKGSTELVPGARCNVSLQDSVGELELFDVDTSQSGEYTCIVSNEAGR 8743 HSP 6 Score: 68.1662 bits (165), Expect = 3.914e-10 Identity = 108/532 (20.30%), Postives = 176/532 (33.08%), Query Frame = 3
Query: 357 LEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIA---HVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREI---------SVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRV------------------------ESEPVTLN----------------------------VQTEKSIILDPQSKGI--SAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQS 1754
L WFKDGK L++ +++ F L I+ + D+G Y ++ G ++ RI P S L +E N L +G+ ME + G P + WF DG + G + CAL + D GDY+C+A N G E C+ E PPSF++ DP ++ S+ +G+P + + W+ + +R L+ ++ L + S +G Y C++ +E GR + +P+ L TEKS IL+ S + S + E + K +I + P F+KQLEP+ ++ GDS +L ++ A + V WYK + + + ++ + ATL S DSG+Y A N G+ S
Sbjct: 8503 LSTKWFKDGKELTSDNKYKISFFNKVSGLKIINVVPGDSGVYSFEVQNPVGKDSCKVSIQVSDRIIPPSFTRKL--------KETNGL-----SGSSVVME--------------------CKVYGSP-------------PISVLWFHDGNEISSGRKYQTTLTDNTCALTVNMLEEADAGDYTCIATNVAGSDE---CSAPLTVRE-------------------------------------PPSFVQKPDPMDVLTGSNVTFTSIVKGSPPF-------------TVSWFKGSTELVPGARCNVSLQDSVGELELFDVDTSQSGEYTCIVSNEAGRASCTTRLFVKAPAIFVKRLNDYSIEKGKPLILEGTFSGTPPISVTWKKNGINVIASQRCNITTTEKSAILEILSSTVEDSGQYNCYIENASGKDSCSAQILILEPPYFVKQLEPV-KVTVGDSASLQCQL--AGTPEIGVSWYKGDTKLRPTATCKMHFKNNVATLVFTQVDSSDSGEYICRAENSVGEVSS 8932 HSP 7 Score: 67.781 bits (164), Expect = 5.724e-10 Identity = 122/601 (20.30%), Postives = 221/601 (36.77%), Query Frame = 3
Query: 354 SLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQ-----LPEEMMQEINNLD----------------------KNYETGTYHGMEEDKHAAK-----------------TIPKFLSQL-------PNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPI--DPREISVSEGAPA----YFKVTIDPGNGQKLSND-----------------------WYHNGEIVKTCSRLTGKLEMNISA-----LNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGIS-AIHALEAELNAPKAEMKE---DEIITDLPSFIKQLEPIDQLNEGDSVT-LTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSE-KINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNIST 1883
+ E+ W+ +G L S+ G + L+IL +D G Y V ++ G A S+ A + + + Y +Q +P+ + E+ + K+ T T + +H A T+ K + L P S+T+ +G+ + + W ++G + +R V + +I+ D G+YS V NS GK E Q + K+ + E +++ +++P E + +T P EP+ P+ +S +E P + V+ P Q L + WY +G+ +K G + + SA L I SD G Y+C + E G ++ Q+ KSI Q IS +++ +P+A+ +E E I+ P + L D DSV T+KV + + W K+ I GS+ ++S ++ L I+ + D G Y TN G S K+ I + + S+ Q+ +E+++ +++
Sbjct: 34191 TAEVKWYHNGVELQESSKIHYTNTSGVLTLEILDCQTEDGGTYRAVCTNYKGEA-SDYATLDVTGGAYTTYASQRRDEEVPKSVFPELTKTEAYAVSSFKRTSELEAASSVREVKSQMTETRESLSTYEHYASAEMKSATSEEKSLEEKATVRKIKTTLAARILTKPRSITVHEGESARFS--CDTDGEPVPTVTWLREGQVVSTSARHQVTTTKYKSTFEISSVQASDEGNYSVVVENSDGKQEAQFT--------LTVQKARVIEKAVTSPPRVKSP-EPRVKSPETVKSPKRVKSPEPVTSHPKAVSPTETKPTEKGQHLPVSAPPKITQSLKAEASKDIAKLTCAVESSALCAKEVAWYKDGKKLKE----NGHFQFHYSADGTYELKIHNLSESDCGEYVCEVSGEGGTSKTS-FQFTGQSFKSI--HEQVSSISETTKSVQKTAESPEAKKQEPIAPESISSKPVIVTGLR--DTTVSSDSVAKFTIKV--TGEPQPTITWTKDGKAIAQGSKYKLSSNKEEFILEILKTETSDGGLYACTVTNSAGSVSSSCKLTIKAVKDTEAQKVSTQKTSEVTSQKKASAQEEISQKALTS 34768 HSP 8 Score: 67.3958 bits (163), Expect = 6.334e-10 Identity = 98/497 (19.72%), Postives = 163/497 (32.80%), Query Frame = 3
Query: 285 SELV-VDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYH-NGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINI 1769
+EL+ V G+P L V + L+ W+KDG+ L A ++ F L D+G Y + G++ T L ++ +N ++ D A ++P +S WFKDG L R + + G +L+I+ D G+++C A NS+G ++ ++ PPSF+ P V G+ K L+ W+ + E+V S K E + S+L + SD+G+Y C + + G VE D + + +FI++LEP L +GD L KV + + W+ N+ + S+ ++S+ TA L + EDSG+Y A N+ G I I
Sbjct: 4911 AELIQVTAGDPATLEYTVSGTPE--LKPKWYKDGRPLVASKKYRISFKNNIAQLKFYSAELHDSGQYTFEISNEVGSSSCETT------------FTVLDRDIAPLFTKPLRNVDSVVGGACRLDCKIAGSLPMRVS-------------------------------WFKDGKELTASDRYQIAFVEGTASLEISRVDMNDAGNFTCRATNSVGSKDSSGA----------------------------------------LIVQEPPSFV--TKPGSRDVLPGSAVCLKSAFQ--GSAPLTIKWFKGDKELVSGGSCYITK-ETSESSLELYAVKTSDSGTYTCKVSNVAGSVECS---------------------------------------ADLFVKEPATFIEKLEPSQLLKKGDGTQLACKV--TGTPPIKITWFANDRELRESSKHKMSFAESTAVLRLTDVAIEDSGEYMCEAQNEAGSDHCTGIVI 5276 HSP 9 Score: 63.5438 bits (153), Expect = 1.099e-8 Identity = 141/701 (20.11%), Postives = 238/701 (33.95%), Query Frame = 3
Query: 291 LVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYV-----------------------------------------CVAKSH-SGTAQSNIA--HVRINPESGVDYLTQLPEEMMQ-EINNLDKNYETGTYHGM------EEDKHAAKTI---PKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECL-PNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLTV-----IPMVDFRP 2213
+ V GE L +V A L + W+KDGKLL++ + F L IL ++D G Y C+ + +G++ +IA H + SG Y T + + ++N+LD + + G+Y + ++ A T+ P F+ + P L ++ G+ + + WF+ L G+R ++ ++ L++ +G+Y+CV N+ G+A SC +E L++ S+ KS+ G T +P ISV+ W +G + R L IL + D G Y C + +E GR A AL + L P F+ +LEP++ + GDSV+L +V A + V W+K + + + + + ATL DSG+Y +A N G S+ I F+ +QE L P+F + +I G P +++G + W D VL+ D + ++ L+I + + +A+NPLG A A LT P D +P
Sbjct: 6601 MTVTVGETCALECKV--AGTPELSVEWYKDGKLLTSSQKHKFSFYNKISSLKILSVEKEDAGTYTFQVQNNVGKSSCTAVVDVSDRMVPPSFTRRLKDTGGVLGTSCILECKVAGSSPISIAWFHEKTKIVSGAKYQTTFSDNVCTLQLNSLDSS-DMGSYTCVAANVAGSDECRALLTVQEPPSFVKE-PEPLEVLPGKNITFTSVI--RGTPPFKVGWFRGARELVKGNRCNIYFEDTVAELELFNIDISQSGEYTCVVSNNAGQA---SCTTRLFVKEPATFVKKLSDHSVEPGKSIILEG------TYTGTLP-------------ISVT----------------------WKKDGVSITPSERCNIVTTEKTCILEILSSTKGDAGHYSCEIENEAGR-------------------------DACDALVSTLEP--------------PYFVTELEPLEA-SVGDSVSLQCQV--AGTPEITVSWFKGDTKLRSTPEYRTYFTNNVATLVFNKVSINDSGEYTCMAENSIGTAASKTI----------------------------------------FR----IQERQLPPSFARQLKDIEQTVGLPVTLTCRLNGSAPIQVCWYRDGVLLRDDENLQMSFVDNVATLKILQTDLSHSGQYSCSASNPLGTASSTARLTAREPKKSPFFDIKP 7165 HSP 10 Score: 63.1586 bits (152), Expect = 1.323e-8 Identity = 82/315 (26.03%), Postives = 134/315 (42.54%), Query Frame = 3
Query: 294 VVDEGEPLRLMTRVGPADDGSLEINWF-KDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRIN-PESGV-DYLTQLPEEMMQEINNL---DKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQG-QPLHIEIFLEHHSDSSLVTE------------------WFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTI 1163
V EG P R +V D L+++W+ KD K+ + T F+ + L+I +D G Y VA + G S+ A +R+ PES + + + Q E M+EI +L +++++ TY +A +T+ + LS+ P T Q +F+ SD + E WF +GM L + ++D +L I F +D G+Y+C+A N GKA S ++ P TE + K+LE P P PP F + + P + G PA F+ ++
Sbjct: 3361 VTSEGRPARFQCQVSGTD---LKVSWYCKDKKIKPSRFFRMTQFEDTY-QLEIAEAYPEDEGTYAFVANNAVGQV-SSTATLRLEAPESILHERIGQQIEMEMKEIASLLSAEEDFQ--TYSSDLRLPNANETL-ELLSEPPARSTQFDSRQEGAAPVFIREISDVEISVEDVAKLSVTVTGCPKPKIQWFFNGMLLTPSADYKFVFDGDTHSLIILFTRFQDEGEYTCLASNEYGKA-VCSAHLRISPR-----GERSTEMESGEKKALEKPKG-----------PCPPYFFKELKP--VHCGPGIPAVFEYSV 3648 HSP 11 Score: 61.6178 bits (148), Expect = 3.963e-8 Identity = 96/466 (20.60%), Postives = 160/466 (34.33%), Query Frame = 3
Query: 363 INWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNS--LYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQS 1754
++WFK L G+R + L++ +G+Y C+ + +G A T+L K F+ +L N ++ +G+PL +E S+ W K+G+++ R ++ L+I ED+G Y+C N+ GK SC+ +Q+ LE PP F++ ++P +++V + A ++ P ++ WY ++ + + N++ L SD+G YIC + G V S L VQ +K PSF +QL + + +V L V E A S + V WYK+ + + +Q S+ ATLNI SG Y ATN G S
Sbjct: 8694 VSWFKGSTELVPGARCNVSLQDSVGELELFDVDTSQSGEYTCIVSNEAGRASCT---------------TRL----------------------------FVKAPAIFVKRL-NDYSIEKGKPLILEGTFSGTPPISVT--WKKNGINVIASQRCNITTTEKSAILEILSSTVEDSGQYNCYIENASGK---DSCS--------------------AQILILE-----------------PPYFVKQLEPVKVTVGDSASLQCQLAGTP----EIGVSWYKGDTKLRPTATCKMHFKNNVATLVFTQVDSSDSGEYICRAENSVGEVSSS-TFLTVQEQKL-----------------------------------PPSFSRQLRDVQE-----TVGLPVVFECAVSGSEPISVSWYKDGKPLKDSPNIQTSFLDNIATLNIFKTDRSLSGQYSCTATNPIGSASS 9028 HSP 12 Score: 60.4622 bits (145), Expect = 8.258e-8 Identity = 63/281 (22.42%), Postives = 119/281 (42.35%), Query Frame = 3
Query: 108 YNNNSNFNSVPDEGVIPNGTTMAPHALEVVNQVNKRSLASPR--RGSDHLYDSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYE---TGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDR--GYCALDIAFCYPEDNGDYSCVARNSLGK 929
Y + + + GV +G+T+ EV+ + + R ++ PT L++ V+ EGE + L + + S ++ W+++ + + F F G L I +D+G + C A + +GT ++ ++ + D T L E+ E ++ + T T E+ P F+S+ P L++G + E + + + W K G+ L G R V Y++ G C L I+ + +D G+Y+ V RN G+
Sbjct: 893 YKSQAGIEVKKEVGVSISGSTVREEHFEVLRGREAKVTETARVPAPAEVPVTPPTLVSGLKNVTVI-EGESVTLECHI--SGYPSPKVTWYREDYQIESSIDFQITFQGGIARLMIREAFAEDSGRFTCSAVNEAGTVSTS-CYLAVQVSEEFDKETTLTEKFATEEKRFVESRDVVMTDTSITEEQAGPGEPAAPFFISK-PVVQKLVEGGSVVFECQIGGNPKPHVY--WKKSGVPLTTGYRYKVSYNKQTGECRLVISMTFADDAGEYTIVIRNKHGE 1166 HSP 13 Score: 57.7658 bits (138), Expect = 5.848e-7 Identity = 78/331 (23.56%), Postives = 136/331 (41.09%), Query Frame = 3
Query: 978 ITKSNLTESSISQLKSLENPGEEYARYNDTSVIPM-PPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITD---------LPSFIKQLEPIDQ-LNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAIS--EDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRL-KDLEEDLASGNISTFQNTEDVQEECLPNF 1928
I+ S + E L+ E E AR + +P+ PP+ + + + ++V EG + I K++ WY +++ + I+ L I D+G + C +E G V S L VQ + D ++ E + + D IT+ P FI + P+ Q L EG SV ++ + +V W K+ + G R ++SY++ T +V +++ +D+G+Y ++ NK+G+T +AS+ L + A Y+ L K +E L +STF V E P F
Sbjct: 909 ISGSTVREEHFEVLRGREAKVTETARVPAPAEVPVTPPTLVSGL--KNVTVIEGESVTLECHISGYPSPKVT--WYREDYQIESSIDFQITFQGGIARLMIREAFAEDSGRFTCSAVNEAGTV-STSCYLAVQVSEE--FDKETTLTEKFATEEKRFVESRDVVMTDTSITEEQAGPGEPAAPFFISK--PVVQKLVEGGSVVFECQI--GGNPKPHVYWKKSGVPLTTGYRYKVSYNKQTGECRLVISMTFADDAGEYTIVIRNKHGET----------------SASASLLEEADYEALVKTQQEMLYQTQMSTFIQEPKVG-EIAPGF 1211 HSP 14 Score: 57.3806 bits (137), Expect = 7.596e-7 Identity = 111/484 (22.93%), Postives = 187/484 (38.64%), Query Frame = 3
Query: 1110 PREISVSEGAPAYFKVTIDPGNGQKLSN-DWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQT----EKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLE--PIDQ-----LNEGDSVTLTVKVEPANDNSLY-------------VEWYKNENHIINGSRMQISYDR-GTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLP-NFDVKPM------NITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVHVA-ATNPLGVAKEEATLTVIPMVDFRPDLRHITPENPFKKMAGLKKVECTSE----LNDAFKKSKPTAERIVKIEMGS-ETKSKFYKSPDVIETEKLYDQVAAGLRKNI 2444
PR I+V EG A F D G+ + W G++V T +R S I SD G+Y V+ + G+ E++ TL VQ EK++ P+ K + +PK +K E +T P + E P ++ ++ +T ++K E + D + V WYK+ + Q Y GT L I + D G+Y + + G +++ F G+ + ++++ + E S + QE P + KP+ + T AKF +KV+G P PT+ W D + S K ++ LEI + +E D ++ TN G LT+ + D +K++ K E TS+ + + T+E I E+ S ET + ++ V+ E++ AA L K+I
Sbjct: 34356 PRSITVHEGESARFSCDTD---GEPVPTVTWLREGQVVSTSARHQVTTTKYKSTFEISSVQASDEGNYSVVVENSDGKQEAQ-FTLTVQKARVIEKAVTSPPRVKSPEPRVKSPETVKSPK-RVKSPEPVTSHPKAVSPTETKPTEKGQHLPVSAPPKITQSLKAEASKDIAKLTCAVESSALCAKEVAWYKDGKKLKENGHFQFHYSADGTYELKIHNLSESDCGEYVCEVSGEGGTSKTS----FQFTGQSFKSI---------HEQVSSISETTKSVQKTAESPEAKKQEPIAPESISSKPVIVTGLRDTTVSSDSVAKFTIKVTGEPQPTITWTKDGKAIAQGSKYKLSSNKEEFILEILK-TETSDGGLYACTVTNSAGSVSSSCKLTIKAVKD-----------TEAQKVSTQKTSEVTSQKKASAQEEISQKALTSEEIKMSEVKSHETLAIKEEASKVLIAEEVKRSAAASLEKSI 34809 HSP 15 Score: 57.3806 bits (137), Expect = 8.264e-7 Identity = 107/498 (21.49%), Postives = 161/498 (32.33%), Query Frame = 3
Query: 279 LESELVVDEGEPLRLMTRVGPADDGSLE----------INWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNG 1742
LE V + EP+++ D SL+ ++W+K L + F L D+G+Y+C A++ G S+ T+ ++E K P F QL + + G P+ E + S+ W+KDG LK I + L+I +G YSC A N +G A + + K LTE PP F P+ P + V E A VT Q + W + +++ N + L I+ D+G Y C +E G+ + A+LN +KE I PSF K+L + EG+S L +V A + V WYKN I S +I + L + A D+G Y ATN G
Sbjct: 8846 LEPPYFVKQLEPVKVTV----GDSASLQCQLAGTPEIGVSWYKGDTKLRPTATCKMHFKNNVATLVFTQVDSSDSGEYICRAENSVGEVSSS------------------------------------TFLTVQEQKLP----PSFSRQLRDVQETV-GLPVVFECAVSGSEPISV--SWYKDGKPLKDSPNIQTSFLDNIATLNIFKTDRSLSGQYSCTATNPIGSASSGA-------------KLILTEGK------------------------NPPFFDIPLAPMDAVVGESADLECHVT----GTQPIKVTWAKDNREIRSGGNYQISYLENSAHLTIVKVDKGDSGQYTCYAVNEVGK----------------------------DSCTAQLN-----IKERLI---PPSFTKKLSETVEETEGNSFKLEGRV--AGSQPITVAWYKNNVEIHPTSNCEIMFKNNALLLQVKRASMADAGLYTCKATNDAG 9217 HSP 16 Score: 56.9954 bits (136), Expect = 8.992e-7 Identity = 57/223 (25.56%), Postives = 95/223 (42.60%), Query Frame = 3
Query: 360 EINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLH----IEIFLEH--HSDSSLVTEWFKDGMSLKLGSRIDVIYD-RGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESS 1007
+I WF +G LL+ + + +FD L IL+T D G+Y C+A + G A + AH+RI+P E E+ E+G +E+ K P F +L +P+H I E+ H + + WFK+ M L +I+ G + D+G Y C A+N G++ T + + PE++ + ++ E S
Sbjct: 3539 KIQWFFNGMLLTPSADYKFVFDGDTHSLIILFTRFQDEGEYTCLASNEYGKAVCS-AHLRISPRG----------ERSTEM-------ESGEKKALEKPKGPCP--PYFFKEL---------KPVHCGPGIPAVFEYSVHGEPAPTVLWFKEDMPLYTSVCYTIIHSPDGSGTFIVNDPQRGDSGLYLCKAQNLWGES-TCAAELLVLPEDTDVPDASCKEES 3731 HSP 17 Score: 55.0694 bits (131), Expect = 3.772e-6 Identity = 48/187 (25.67%), Postives = 81/187 (43.32%), Query Frame = 3
Query: 1476 EIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEG----EKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEWR 2024
E+ P+ + L+ + + EG+SVTL + + S V WY+ + I + QI++ G A L I A +EDSG + A N+ G ++ SC+ E+ ++L A ++ D+ + S + E P F KP+ EGG F ++ G P P + W+
Sbjct: 940 EVPVTPPTLVSGLKNVTVI-EGESVTLECHI--SGYPSPKVTWYREDYQIESSIDFQITFQGGIARLMIREAFAEDSGRFTCSAVNEAGT-----VSTSCYLAVQVSEEFDKETTLTEKFATEEKRFVESRDVVMTDTSITEEQAGPGEPAAPFFISKPVVQKLVEGGSVVFECQIGGNPKPHVYWK 1118
BLAST of Titin vs. UniProt/SwissProt
Match: sp|Q8WZ42|TITIN_HUMAN (Titin OS=Homo sapiens OX=9606 GN=TTN PE=1 SV=4) HSP 1 Score: 107.842 bits (268), Expect = 3.093e-22 Identity = 179/768 (23.31%), Postives = 304/768 (39.58%), Query Frame = 3
Query: 360 EINWFKDGKLLSAGSRFSTIF-DRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPE-EMMQEINNLDKNYETGTYHGMEEDKHA------AKTIPKFLS-----------QL-----------PNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSL--KLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLE--MNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPI------DQLNEGD-----------------SVTLT--------VKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLTVIPMVDFRPDLRHITPE--------NPFKKMAGLKKVEC---TSELNDAFKKSKPTAERIV--KI-EMGSETKSKFYKSPDVIETEKLYDQVAA 2426
+I W+KDGK + G R+ F G L I + +D G Y A + G A + + + P + + T +P E + I +L + + M + + A+ P +S QL P S ++GQ ++ + T WF DG + ++ VI + G +L I P D+G+++ VA+N G+ SSIS + ++E + + P F+E + + +++ EG+ KV + W N +I+ ++E +AL I T+ D+ Y ++ GR ++ +NV+ E +P+ E +L P+ + EI +LEP+ +Q EGD S+ L ++ P D ++ VEW + + +R+++ + G +L+ A S DSG ATNK G + I E + +V S L G QR+++LE G ++ T D +E+ P+ + P + EG A+F +V+G+P P + W ++ L+ + DG I+YL+I C +V V A NP GV + + L + DFR LR PE P K ++KV+ T+E + K + AERI K+ E E +SKF + + E Y+ + A
Sbjct: 1321 KIAWYKDGKRIKHGERYQMDFLQDGRASLRIPVVLPEDEGIYTAFASNIKGNAICS-GKLYVEPAAPLGAPTYIPTLEPVSRIRSLSPRSVSRSPIRMSPARMSPARMSPARMSPARMSPGRRLEETDESQLERLYKPVFVLKPVSFKCLEGQTARFDLKVVGRPMPE--TFWFHDGQQIVNDYTHKV-VIKEDGTQSLIIVPATPSDSGEWTVVAQNRAGR------------------------SSISVILTVEAVEHQ-----------VKPMFVEKL--KNVNIKEGSRLEMKVRATGNPNPDIV--WLKNSDIIVPHKYPKIRIEGTKGEAALKIDSTVSQDSAWYTATAINKAGR-DTTRCKVNVEVE---FAEPEP---------ERKLIIPRGTYRAKEIAAP------ELEPLHLRYGQEQWEEGDLYDKEKQQKPFFKKKLTSLRLKRFGPAHFECRLTPIGDPTMVVEWLHDGKPLEAANRLRMINEFGYCSLDYGVAYSRDSGIITCRATNKYGTDHTSATLIVKDE-KSLVEESQLPEGRKGLQRIEELERMAHEGALTGV--TTDQKEKQKPDIVLYPEPVRVLEGETARFRCRVTGYPQPKVNWYLNGQLIRKSKRFRVRYDG-IHYLDIVDCKSYDTGEVKVTAENPEGVIEHKVKLEIQQREDFRSVLRR-APEPRPEFHVHEPGKLQFEVQKVDRPVDTTETKEVVKLKR--AERITHEKVPEESEELRSKFKRRTE----EGYYEAITA 2015 HSP 2 Score: 79.337 bits (194), Expect = 1.415e-13 Identity = 110/477 (23.06%), Postives = 190/477 (39.83%), Query Frame = 3
Query: 678 PKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRV--------ESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEM-KEDEIITDL------------------PSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNF--DVKPMNITAGEGGPAKFLVKVSGFPTPTLEW 2021
P+ L +L +T+ G+P + + W+K+ L G + ++D L + +PED Y+C A+N G A T + PE + +P +E Y PP+ I P+ ++ SEG PA F+ + +G L WY + +K + L I P D G+Y V + G+V E+ L+ + E+ I ++ + S + A E L++ + ++ K +E + L P FIK++ D ++ GD TL+V V ++W+ N + + + +D +L I+ ED G+Y +A+N G+T + ++ L +S G + KD E + A ++ E + C P+F ++KP I +G PA F V G P PT+ W
Sbjct: 3239 PQVLQEL-QPVTVQSGKPARFCAVISGRPQPKI--SWYKEEQLLSTGFKCKFLHDGQEYTLLLIEAFPEDAAVYTCEAKNDYGVATTSASLSVEVPE-------------------VVSPDQEMPVY--------PPAIITPL--QDTVTSEGQPARFQCRV---SGTDLKVSWYSKDKKIKPSRFFRMTQFEDTYQLEIAEAYPEDEGTYTFVASNAVGQVSSTANLSLEAPESILHERIEQEIEMEMKEFSSSFLSAEEEGLHSAELQLSKINETLELLSESPVYPTKFDSEKEGTGPIFIKEVSNAD-ISMGDVATLSVTV--IGIPKPKIQWFFNGVLLTPSADYKFVFDGDDHSLIILFTKLEDEGEYTCMASNDYGKT---------------ICSAYLKINSKG-EGHKDTETESAVA-----KSLEKLGGPCPPHFLKELKP--IRCAQGLPAIFEYTVVGEPAPTVTW 3654 HSP 3 Score: 77.0258 bits (188), Expect = 7.737e-13 Identity = 98/495 (19.80%), Postives = 174/495 (35.15%), Query Frame = 3
Query: 345 DDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAG 1829
+D + + W KDG+ L G + F+ L+I D+G YVC A + +G++ + P P F+ ++ S ++ G+ + L+ + WFK+ L + + + + LDI ED+G YSC A N +G + SC+ VI PPSFI+ ++P +I G A + + G G W+ + + +++ + + ++ L I +D G Y CV+ +E G+ G A H L+ + P+F+K+++ + L G +VTL V + V W K + I ++++S+ G A L I G Y +A N+ G +Q+ + E KI+ + L+ +AG
Sbjct: 4596 EDRKVTVTWSKDGQKLPPGKDYKICFEDKIATLEIPLAKLKDSGTYVCTASNEAGSSSCSATVTVREP-------------------------------------------PSFVKKVDPSYLMLPGESARLHCKLK--GSPVIQVTWFKNNKELSESNTVRMYFVNSEAILDITDVKVEDSGSYSCEAVNDVG---SDSCSTEI-------------------------------------VIKEPPSFIKTLEPADI--VRGTNALLQCEVS-GTGP-FEISWFKDKKQIRSSKKYRLFSQKSLVCLEIFSFNSADVGEYECVVANEVGKC----------------------GCMATHLLK-----------------EPPTFVKKVDDLIALG-GQTVTLQAAVR--GSEPISVTWMKGQEVIREDGKIKMSFSNGVAVLIIPDVQISFGGKYTCLAENEAG-SQTSVGELIVKEPAKIIERAELIQVTAG 4958 HSP 4 Score: 70.8626 bits (172), Expect = 6.422e-11 Identity = 77/355 (21.69%), Postives = 127/355 (35.77%), Query Frame = 3
Query: 678 PKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNG 1742
P + + P +T+ G P +E + + L +WFKDG L S+ + + +L I D G YS +NS+GK+ +C V+ H + I+ PPSFI + ++++ GA + + +S W+ +G + + + N+ LN+ PSDTG Y CV + G E V L VQ + PSF + + ++ L G S+T T + V+W+K ++ G IS + L + +SGDY + TN G
Sbjct: 7571 PARIIEKPEPMTVTTGNPFALECVVTGTPE--LSAKWFKDGRELSADSKHHITFINKVASLKIPCAEMSDKGLYSFEVKNSVGKS---NCTVSVHVSDRIV----------------------------------PPSFIRKL--KDVNAILGASVVLECRV--SGSAPISVGWFQDGNEIVSGPKCQSSFSENVCTLNLSLLEPSDTGIYTCVAANVAGSDECSAV-LTVQ--------------------------------------EPPSFEQTPDSVEVL-PGMSLTFTSVIR--GTPPFKVKWFKGSRELVPGESCNISLEDFVTELELFEVQPLESGDYSCLVTNDAG 7840 HSP 5 Score: 69.3218 bits (168), Expect = 1.725e-10 Identity = 101/514 (19.65%), Postives = 180/514 (35.02%), Query Frame = 3
Query: 357 LEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPE---------------------------EMMQEINNLDKNYE---------------TGTY-------HGMEEDKHAAKTI-PKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVE-SEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQ 1745
++W+KD + L +G ++ + + + IL D G+Y C A + G+ + P V L+ + E+++E +N+ +Y G Y GM+E + P + + P S+ + G +E + + L T+WFKDG L ++ + + L I P D+G YS +N +GK SC + +D +V PPSF + +E + G+ + + +S W+H G + + + L N AL + SD+G Y C+ + G E S P+T + + PSF+++ +P+D L G +VT T V+ V W+K + ++ G R +S + A L + + SG+Y I +N+ G+
Sbjct: 8355 FHVSWYKDKRELRSGKKYKIMSENFLTSIHILNVDAADIGEYQCKATNDVGSDTCVGSIALKAPPRFVKKLSDISTVVGKEVQLQTTIEGAEPISVVWFKDKGEIVRESDNIWISYSENIATLQFSRVEPANAGKYTCQIKNDAGMQECFATLSVLEPATIVEKPESIKVTTGDTCTLECTVAGTPE--LSTKWFKDGKELTSDNKYKISFFNKVSGLKIINVAPSDSGVYSFEVQNPVGK---DSCTASLQ-------------------------------VSDRTV---PPSFTRKL--KETNGLSGSSVVMECKV--YGSPPISVSWFHEGNEISSGRKYQTTLTDNTCALTVNMLEESDSGDYTCIATNMAGSDECSAPLT----------------------------------------VREPPSFVQKPDPMDVLT-GTNVTFTSIVK--GTPPFSVSWFKGSSELVPGDRCNVSLEDSVAELELFDVDTSQSGEYTCIVSNEAGK 8782 HSP 6 Score: 67.781 bits (164), Expect = 5.723e-10 Identity = 121/577 (20.97%), Postives = 210/577 (36.40%), Query Frame = 3
Query: 180 HALEVVNQVNKRSLASPRRGSDHLYDSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHS----------------------------GTAQSNIAHVRINPESGVDYLTQ----LP--------EEMMQEINNLDKNY-ETGTYHGMEEDKHAA---------KTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNS--LYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQS 1754
++ EV N V K S + + SD P+F + L+ E G + + +V + ++WF +G +S+G ++ T L + E D+GDY C+A + + GT + + V+ P V + +P E+ + E+ D + ++G Y + ++ K KF+ +L N ++ +G+PL +E S+ W K+G+++ R ++ L+I ED G Y+C N+ GK SC+ +Q+ LE PP F++ ++P ++SV + A ++ P ++ WY ++ + N++ L +D+G YIC + G V S L VQ +K PSF +QL + + +V L V + A S + V WYK+ + + +Q S+ TATLNI +G Y ATN G S
Sbjct: 8583 YSFEVQNPVGKDSCTASLQVSDRTV-PPSFTRKLK-ETNGLSGSSVVMECKV--YGSPPISVSWFHEGNEISSGRKYQTTLTDNTCALTVNMLEESDSGDYTCIATNMAGSDECSAPLTVREPPSFVQKPDPMDVLTGTNVTFTSIVKGTPPFSVSWFKGSSELVPGDRCNVSLEDSVAELELFDVDTSQSGEYTCIVSNEAGKASCTTHLYIKAPAKFVKRL-NDYSIEKGKPLILEGTFTGTPPISVT--WKKNGINVTPSQRCNITTTEKSAILEIPSSTVEDAGQYNCYIENASGK---DSCS--------------------AQILILE-----------------PPYFVKQLEPVKVSVGDSASLQCQLAGTP----EIGVSWYKGDTKLRPTTTYKMHFRNNVATLVFNQVDINDSGEYICKAENSVGEV-SASTFLTVQEQKL-----------------------------------PPSFSRQLRDVQE-----TVGLPVVFDCAISGSEPISVSWYKDGKPLKDSPNVQTSFLDNTATLNIFKTDRSLAGQYSCTATNPIGSASS 9067 HSP 7 Score: 65.855 bits (159), Expect = 1.914e-9 Identity = 100/497 (20.12%), Postives = 165/497 (33.20%), Query Frame = 3
Query: 285 SELV-VDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYH-NGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINI 1769
+EL+ V G+P L V A L+ W+KDG+ L A ++ F L D+G Y + G++ T+ ++ D P F L N +++ G + + + WFKDG + R + + G +L+I D G+++C A NS+G ++ ++ PPSF+ P V G+ K T L+ W+ N E+V S K + S+L + SD+G+Y C + + G VE A L + + +F+++LEP L +GD+ L KV + + W+ N+ I S+ ++S+ TA L + EDSG+Y A N+ G I I
Sbjct: 4950 AELIQVTAGDPATLEYTV--AGTPELKPKWYKDGRPLVASKKYRISFKNNVAQLKFYSAELHDSGQYTFEISNEVGSSSCET-----------------------------------TFTVLDRD-----IAPFFTKPLRNVDSVVNGT---CRLDCKIAGSLPMRVSWFKDGKEIAASDRYRIAFVEGTASLEIIRVDMNDAGNFTCRATNSVGSKDSSGA----------------------------------------LIVQEPPSFV--TKPGSKDVLPGSAVCLKSTFQ--GSTPLTIRWFKGNKELVSGGSCYITKEALE-SSLELYLVKTSDSGTYTCKVSNVAGGVE----------------------------CSANL-----------FVKEPATFVEKLEPSQLLKKGDATQLACKV--TGTPPIKITWFANDREIKESSKHRMSFVESTAVLRLTDVGIEDSGEYMCEAQNEAGSDHCSSIVI 5315 HSP 8 Score: 63.5438 bits (153), Expect = 1.001e-8 Identity = 148/712 (20.79%), Postives = 224/712 (31.46%), Query Frame = 3
Query: 291 LVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHH--SDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREI---------SVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVE-------SEPVTL-------NVQTEKSIILDPQSKGISAIHALEAE------------------------LNAPKAEMK------EDEIITDL----------PSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECL-PNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLTV-----IPMVDFRP 2213
+ V GE L +V A L + W+KDGKLL++ + F L IL D G Y Q NN+ K+ T D P F +L N+ G L LE S + WF + + G++ + C L + D G+Y+CVA N G E C +E PPSF++ +P E+ SV G P FKV +W+ + R E ++ L + S +G Y CV+ + G+ EP +V+ KSIIL+ G I + LN+ K + E+E D+ P F+ +LEP++ GDSV+L +V A + V WYK + + + + ATL DSG+Y A N G S+ + F+ +QE L P+F + +I G P +++G + W D VL+ D +T ++ L+I + + +A+NPLG A A LT P D +P
Sbjct: 6640 MTVTVGETCTLECKV--AGTPELSVEWYKDGKLLTSSQKHKFSFYNKISSLRILSVERQDAGTYT-----------------------------------FQVQNNVGKSSCTAVV-----DVSDRAVPPSFTRRLKNT-----GGVLGASCILECKVAGSSPISVAWFHEKTKIVSGAKYQTTFSDNVCTLQLNSLDSSDMGNYTCVAANVAGSDE---CRAVLTVQE-------------------------------------PPSFVKEPEPLEVLPGKNVTFTSVIRGTPP-FKV------------NWFRGARELVKGDRCNIYFEDTVAELELFNIDISQSGEYTCVVSNNAGQASCTTRLFVKEPAAFLKRLSDHSVEPGKSIILESTYTGTLPISVTWKKDGFNITTSEKCNIVTTEKTCILEILNSTKRDAGQYSCEIENEAGRDVCGALVSTLEPPYFVTELEPLEAAV-GDSVSLQCQV--AGTPEITVSWYKGDTKLRPTPEYRTYFTNNVATLVFNKVNINDSGEYTCKAENSIGTASSKTV----------------------------------------FR----IQERQLPPSFARQLKDIEQTVGLPVTLTCRLNGSAPIQVCWYRDGVLLRDDENLQTSFVDNVATLKILQTDLSHSGQYSCSASNPLGTASSSARLTAREPKKSPFFDIKP 7204 HSP 9 Score: 57.7658 bits (138), Expect = 5.653e-7 Identity = 49/207 (23.67%), Postives = 88/207 (42.51%), Query Frame = 3
Query: 360 EINWFKDGKLLSAGSRFSTIFDR-GFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKF---LSQLPNSLTLIQGQPLH----IEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVT 956
E+ W+KDGK L F + G L I E D G+YVC GT+++N+ ++ Q + + ++++ + + ++ E KT PK +S P +T +Q + + ++ + W KDG ++ G + + D+G L+I D+G Y+C +NS G + SC +T
Sbjct: 33675 EVTWYKDGKKLKENGHFQFHYSADGTYELKINNLTESDQGEYVCEISGEGGTSKTNL-----------QFMGQAFKSIHEKVSKISETKKSD--QKTTESTVTRKTEPKAPEPISSKPVIVTGLQDTTVSSDSVAKFAVKATGEPRPTAIWTKDGKAITQGGKYKLSEDKGGFFLEIHKTDTSDSGLYTCTVKNSAGSV-SSSCKLT 33867 HSP 10 Score: 57.7658 bits (138), Expect = 5.896e-7 Identity = 56/209 (26.79%), Postives = 90/209 (43.06%), Query Frame = 3
Query: 1422 IHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCF-------EGEKIVTASSLLADSAGYQRLKDLE-EDLASGNISTFQNTEDVQEECLPNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEWR 2024
+H EA++ EI P+ + L+ + + EG+SVTL + + S V WY+ + I + QI++ G A L I A +EDSG + A N+ G ++ SC+ E EK TA + + + + +E D+ + S + E P F KP+ EGG F +V G P P + W+
Sbjct: 919 LHGREAKVTETARVPAPVEIPVTPPTLVSGLKNVTVI-EGESVTLECHI--SGYPSPTVTWYREDYQIESSIDFQITFQSGIARLMIREAFAEDSGRFTCSAVNEAGT-----VSTSCYLAVQVSEEFEKETTA---VTEKFTTEEKRFVESRDVVMTDTSLTEEQAGPGEPAAPYFITKPVVQKLVEGGSVVFGCQVGGNPKPHVYWK 1116 HSP 11 Score: 57.7658 bits (138), Expect = 6.470e-7 Identity = 85/357 (23.81%), Postives = 134/357 (37.54%), Query Frame = 3
Query: 1077 PMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMK--EDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIV---------------TASSLLADSAGYQ----RLKDLEEDLASGNISTFQNTE-DVQEECL-PNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLVVPDSVCKTYNDG 2078
P PP ++ + P ++V G PA F I K+S WY +++ T + + L ++ P D Y C +++G V + +L+V+ E+ +P EM IIT L + +EG +V + L V WY + I +++ T L I A ED G Y +A+N GQ S N+S E I+ ++S L A+ G +L + E L + S T+ D ++E P F + N G A V V G P P ++W + VL+ P + K DG
Sbjct: 3236 PEPPQVLQELQP--VTVQSGKPARFCAVISGRPQPKIS--WYKEEQLLSTGFKCKFLHDGQEYTLLLIEAFPEDAAVYTCEAKNDYG-VATTSASLSVEV--------------------PEVVSPDQEMPVYPPAIITPLQDTV--------TSEGQPARFQCRV---SGTDLKVSWYSKDKKIKPSRFFRMTQFEDTYQLEIAEAYPEDEGTYTFVASNAVGQVSSTA-NLSLEAPESILHERIEQEIEMEMKEFSSSFLSAEEEGLHSAELQLSKINETLELLSESPVYPTKFDSEKEGTGPIFIKEVSNADISMGDVATLSVTVIGIPKPKIQWFFNGVLLTPSADYKFVFDG 3555
BLAST of Titin vs. UniProt/SwissProt
Match: sp|G4SLH0|TTN1_CAEEL (Titin homolog OS=Caenorhabditis elegans OX=6239 GN=ttn-1 PE=1 SV=2) HSP 1 Score: 95.5153 bits (236), Expect = 1.890e-18 Identity = 144/626 (23.00%), Postives = 237/626 (37.86%), Query Frame = 3
Query: 258 SPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQS-NIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDR-GYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVK--VEPANDNSLYVEWYKNENHIINGSRMQIS--------YDRGTATLNIVSAISEDSGDYWLIATNKNGQTQ-SEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNF--DVKPMNITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLVVPDSVCKTYNDGSINY 2090
+P F+ L + V +G L L+ V + I W KD K + ++ + G L I+ +DD G YVC A++ G AQS ++ ++ +DK++ PKFL L N T +G + +E + ++ W+KDG+ L + +R+ DR G L+I D+G+Y+C A NSLGK T C V + ++K+ LT +P I PP P+ + +V+EG +V +D + +WYH+G++V L + ++ L I G Y+C + ++ G VE+ + + + AP A E +T +P+F+K+L+ + G++ T T + PA V W + NG +Q + +D TATL I + E G Y +A N+ G S ++ IS E +KI + LP F ++KP I EG ++G P P + W D+ +V + DG +NY
Sbjct: 16604 APRFRMQLPTPREVPQGADLTLVCSVSGTPHPN--IKWTKDDKPIDMSNK-QVRHENGVCTLHIIGARDDDQGRYVCEAENIHGVAQSFSVVEIK---------------------EAVDKDH----------------VRPKFLEPLVNCSTC-EGNEMVLECCVTGKPIPTIT--WYKDGLKLIIENRMLQYTDRKGVSRLNIMNVVMNDDGEYTCEAVNSLGKDFTH-CTVKVV--DMGLSKTRLTPVRSRSRSRSRSPSVVGGE------IQRPPVVTRPL--ADATVTEGNRELLEVEVDGFPTPTI--EWYHDGKLVAESRTLRTYFDGRVAFLKIYEAHEEHNGQYVCKVSNKLGAVETRAIVV--------------------------VEAPDAA----EHVTQMPTFVKKLQDVVLKTAGETATFTCQSYANPA----AQVVW------LHNGKALQQTKSNYKTRLFDDNTATLVIENVTDELCGTYTAVANNQFGDVHTSAQLTISGSEAKKIAAS--------------------------------------LPYFIIELKP-KINVVEGATLSIQADLNGSPIPEVVWLKDNSELVESDRIQMKCDG-VNY 17093 HSP 2 Score: 81.2629 bits (199), Expect = 3.719e-14 Identity = 108/427 (25.29%), Postives = 170/427 (39.81%), Query Frame = 3
Query: 783 EWFKDGMSLKLGS-RIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPG----EEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGE-IVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVE--PANDNSLYVEWYKNENHIINGSRMQISYDRGTA--TLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEWRIDD 2033
+W+KD L + S + D Y+ G L + D G Y+C A N LG T + ++ +E KS + + I + K + EE +I MPP+F + E + G P VT + + DWYHNGE I S K + L+IL +D G + V + G ESE L V +I D Q PSF KQL + + +E D + L V ++ PA + + W++NE+ I + ++ +D G+ +L I+ A +EDSG+Y +A NK G+ + + C E++++ S D + R + Q LP P + +G + VSG P P ++W DD
Sbjct: 16301 QWYKDDKELTVPSMKYDSFYNEGLAELTVKNIVESDAGKYTCRATNDLGSIMTHA-KLSVKADEKKKKKSKTSPAVIEKKKDRKTSKVVVIEE--------MIDMPPNFHHLLQDDEAKI--GEPKILVVT--NTTLPEPTVDWYHNGEHISINDSNYLRKHDKGRYELHILSVDSTDEGKWKAVGKNAFGECESE-AKLTV-----VIPDGQY----------------------------APSFGKQLSDV-KCSESDILKLEVNIQANPAPE----INWFRNESEIEHSQHHRLQFDDGSGNYSLTIIDAYAEDSGEYKCVAKNKIGKAHT----VCCVRIEELLSKRSKKIDGSKAPRFR-------------MQ---------LPT----PREVP--QGADLTLVCSVSGTPHPNIKWTKDD 16643 HSP 3 Score: 78.1814 bits (191), Expect = 3.710e-13 Identity = 139/619 (22.46%), Postives = 234/619 (37.80%), Query Frame = 3
Query: 363 INWFKDGKLLSAG-SRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYC--ALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSN-DWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLN--EGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDR-GTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQ--RLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPM-NITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLTV 2189
++W+ +G+ +S S + D+G L IL D G + V K+ G +S + P+ G Y P F QL + + + L +E+ ++ + + WF++ ++ + +D G +L I Y ED+G+Y CVA+N +GKA T V C E +++K +S + G + R+ + +P PRE V +GA ++ +G N W + + + ++ + E + L+I+ D G Y+C + HG +S S + EA + +D + P F LEP+ + EG+ + L V + WYK+ +I +RM DR G + LNI++ + D G+Y A N G K T ++ G RL + S + S ++Q P +P+ + T EG V+V GFPTPT+EW D LV +TY DG + +L+I E + + +N LG + A + V
Sbjct: 16429 VDWYHNGEHISINDSNYLRKHDKGRYELHILSVDSTDEGKWKAVGKNAFGECESEAKLTVVIPD--------------------------GQY------------APSFGKQLSD-VKCSESDILKLEVNIQANPAPEI--NWFRNESEIEHSQHHRLQFDDGSGNYSLTIIDAYAEDSGEYKCVAKNKIGKAHT----VCCVRIEELLSK-----------RSKKIDGSKAPRFR----MQLPT-------PRE--VPQGADLTLVCSV---SGTPHPNIKWTKDDKPIDMSNKQV-RHENGVCTLHIIGARDDDQGRYVCEAENIHGVAQS---------------------FSVVEIKEA--------VDKDHV---RPKF---LEPLVNCSTCEGNEMVLECCV--TGKPIPTITWYKDGLKLIIENRMLQYTDRKGVSRLNIMNVVMNDDGEYTCEAVNSLG---------------KDFTHCTVKVVDMGLSKTRLTPVRSRSRSRSRSPSVVGGEIQR---PPVVTRPLADATVTEGNRELLEVEVDGFPTPTIEWYHDGKLVAESRTLRTYFDGRVAFLKIYEAHEEHNGQYVCKVSNKLGAVETRAIVVV 16919 HSP 4 Score: 57.3806 bits (137), Expect = 7.363e-7 Identity = 97/472 (20.55%), Postives = 171/472 (36.23%), Query Frame = 3
Query: 786 WFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPR-EISVSEGAPAYFKVTIDPGNGQKLSNDWYHNG-EIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQS-KGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLTVI 2192
W K+G+ L+ G++ + D + L++ D G Y+C A N+ G +TC E ++ E S +Q+ ++ E A P+FIE + R + +++E V+ P S W N ++ R T + ++L + P D G+Y C +E G S +N+Q +DP + +GI + E +++ + ++ +G V L ++ A++ L + W +N+ I++ S L I ED G+Y + A N++G + ++ D +R V +E FD +P + G + KV G P P + W + + N+G L I + K + A NP G A E LTV+
Sbjct: 18042 WDKEGIILETGNKYSIYNDGDFYYLEVHHVSTFDKGFYNCTAANNEG-------IITCTSEIDVLPNK---EDSAAQVAKRKSRKEAKA-----------PNFIEVLPGRSQANLNESLCVECSVSAYPC----ASIIWTRNSVRLLPQADRYTMSYDGECASLKFISVTPGDEGTYACEAVNELG---SAVTNMNLQVSG---VDPNAAEGIPPLFRFE------------------------KIKSVRKVVDGSRVELAAELVQASE-PLQIRWLRNKVTIVDSPSFSYSRSENMVFLTIADVFPEDGGEYTVEAKNQSG-----------------IARCTMQLDVRNNER--------------------SVADEAPRVFDFEPTT-RSDPGVSVELRAKVIGHPDPVISWTKAGQKLNNEEKYMMRNEGDKFILRIANVTRADAGKYELTAINPSGQANAELELTVV 18419 HSP 5 Score: 55.4546 bits (132), Expect = 3.143e-6 Identity = 58/228 (25.44%), Postives = 94/228 (41.23%), Query Frame = 3
Query: 261 PTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMS-LKLGSRIDVIYDRGYCA-LDIAFCYPEDNGDYSCVARNSLGKAET 938
P F + V++E + LRL T + + + +++W K+G +L G+++S D F L++ + D G Y C A ++ G I S +D L N D + +E K P F+ LP + L +E + + +S++ W ++ + L R + YD G CA L P D G Y+C A N LG A T
Sbjct: 18008 PKFMVKPKPRKVLEEYKSLRLKTAI--SGNPMPQVHWDKEGIILETGNKYSIYNDGDFYYLEVHHVSTFDKGFYNCTAANNEGI---------ITCTSEIDVLP----------NKEDSAAQVAKRKSRKEAK-----APNFIEVLPGRSQANLNESLCVECSVSAYPCASII--WTRNSVRLLPQADRYTMSYD-GECASLKFISVTPGDEGTYACEAVNELGSAVT 18206
BLAST of Titin vs. TrEMBL
Match: A0A183MH06 (Uncharacterized protein OS=Schistosoma margrebowiei OX=48269 GN=SMRZ_LOCUS15331 PE=4 SV=1) HSP 1 Score: 603.979 bits (1556), Expect = 0.000e+0 Identity = 314/735 (42.72%), Postives = 460/735 (62.59%), Query Frame = 3
Query: 297 VDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPE--SGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQ-LPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQ-----SKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEE-DLASGNISTFQNTEDVQEECLPNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLVVPDSVCKTYNDGSINYLEINRCSEL-GDIKVHVAATNPLGVAKEEATLTVIPMVDFRPDLRHITPENPFKKMAGLKKVECTSELNDAFKKSKPTAERIVKIEMGSETKSKFYKSPDVIETEKLYDQVAAGLRKN--IGPVSNNG 2465
V EGEP+ L R+ P G L++ WFKDG LSAGSR++T+ +RG LD++YTI +D+G Y CV + G AQS V + PE D++ ++ Q + K YE + + K+ P F Q + +S +++G+P+ IE +L+ SDS+L +W K+G + GSR + + DRG L+I +C ED+G Y CVA N+LG E+Q + C PEE I+TKS L + SI+ LK LE PGE+Y +++V +PPSF ++P + VSE P F V +D GNG +L +W +G++VKT SR+TG+LE+ I++L I Y P+DTG+Y C + +E+G + P L + SII Q KG+ AI A+EA L+AP+ + ED+ + P I+Q + + ++ EG ++ V+VEPA D SL VEW+K+ N + +G+R +++Y+RG A L+++ I EDSG+YW NK GQ +S + + C ++T S+LL S GY +K +EE D G+ ++ ED + + PNFDVKP T GEG P KFLV+VSG PTP L W ++D + DS+ K Y+DG+INYLE+NRC L G K+HV A N LG A+ E LTV+ DFRPDL+H+ PENP+KKM GL+KV+C+ ELN A ++KP+A+ I+++E GSE K++ Y+SP+VIE EK+ DQ+A L+K+ P+ N G
Sbjct: 32 VPEGEPVHLELRIAPP--GDLQVQWFKDGVALSAGSRYNTMCERGLASLDVIYTIPEDSGTYFCVISNPYGHAQSEAVPVNVIPEIDPSQDHIDLQSADLYQATSAPTKEYE-------RDQRSQPKSAPHFTQQPVISSSDVLEGEPVRIEAYLDTTSDSTLQVDWMKNGQPVGTGSRFNAVLDRGLIVLEIGYCLAEDSGLYVCVATNALGHTESQPVELRCQPEERIVTKSILDQQSINHLKQLEEPGEDYDYMINSTVQGIPPSFKANLEPSSVQVSEDEPVIFSVPVDCGNGDRLVIEWKRDGQVVKTGSRITGRLELGIASLKIHYAQPNDTGAYTCHVSTEYGSADCGPSNLLCEATGSIIAASQLPGDKEKGLLAIEAIEANLHAPRGTVWEDDSPHEAPVIIQQPQLVGEIEEGTAIHFDVQVEPAADPSLIVEWFKSGNLLTSGTRFKVAYERGLAILDLLYTIPEDSGEYWCRVENKAGQVESNHVQVLCNPSASVITHSNLLQGSEGYNLIKAIEEVDQPDGD--EYRYVEDEEVDSAPNFDVKPQPATIGEGSPVKFLVRVSGKPTPQLTWYLNDEPIEQDSITKIYSDGAINYLEMNRCPALQGTNKLHVIAQNQLGKAEAETILTVLLAEDFRPDLKHVKPENPYKKMVGLRKVDCSPELNKALTRTKPSAQTIMEMERGSEMKARMYRSPEVIEAEKMLDQLALNLKKSEVKRPLVNGG 755 HSP 2 Score: 191.045 bits (484), Expect = 6.503e-47 Identity = 148/517 (28.63%), Postives = 250/517 (48.36%), Query Frame = 3
Query: 258 SPTF-QQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQL-PNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNL---TESSISQLKSLEN--PGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSII----LDPQSKGISAIHALEAELNAPKAE---MKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAIS-EDSGDYWLIATNKNGQTQSEKI 1763
+P F QQ + S V EGEP+R+ + D +L+++W K+G+ + GSRF+ + DRG IVL+I Y + +D+G YVCVA + G +S +R PE + + L + Q IN+L + E G + + P F + L P+S+ + + +P+ + ++ + LV EW +DG +K GSRI + G +L I + P D G Y+C G A+ N+ C SII S L E + ++++E + D S P +P EI EG +F V ++P L +W+ +G ++ + +R E ++ L++LYT+P D+G Y C + ++ G+VES V + S+I L S+G + I A+E E++ P + EDE + P+F + +P + EG V V+V + + + WY N+ I S +I D L + + + + +IA N+ G+ ++E I
Sbjct: 151 APHFTQQPVISSSDVLEGEPVRIEAYLDTTSDSTLQVDWMKNGQPVGTGSRFNAVLDRGLIVLEIGYCLAEDSGLYVCVATNALGHTESQPVELRCQPEERIVTKSILDQ---QSINHLKQLEEPGEDYDYMINSTVQGIPPSFKANLEPSSVQVSEDEPVIFSVPVDCGNGDRLVIEWKRDGQVVKTGSRITGRLELGIASLKIHYAQPNDTGAYTCHVSTEYGSADCGPSNLLCEATGSIIAASQLPGDKEKGLLAIEAIEANLHAPRGTVWEDDSPHEAPVIIQQPQLVGEI--EEGTAIHFDVQVEPAADPSLIVEWFKSGNLLTSGTRFKVAYERGLAILDLLYTIPEDSGEYWCRVENKAGQVESNHVQVLCNPSASVITHSNLLQGSEGYNLIKAIE-EVDQPDGDEYRYVEDEEVDSAPNFDVKPQPA-TIGEGSPVKFLVRV--SGKPTPQLTWYLNDEPIEQDSITKIYSDGAINYLEMNRCPALQGTNKLHVIAQNQLGKAEAETI 658 HSP 3 Score: 166.007 bits (419), Expect = 1.327e-38 Identity = 137/457 (29.98%), Postives = 211/457 (46.17%), Query Frame = 3
Query: 678 PKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSI----ILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPID-QLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADS-AGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAGEGGPA-KFLVKVSGFPTPTL--EW 2021
P+ L L + +G+P+H+E+ + D L +WFKDG++L GSR + + +RG +LD+ + PED+G Y CV N G A++++ V PE ++ ++ S ++ P +EY R + S P F + V EG P + +D + L DW NG+ V T SR L+ + L I Y L D+G Y+CV + G ES+PV L Q E+ I ILD QS I+ + LE E M + PSF LEP Q++E + V +V V+ N + L +EW ++ + GSR+ + G A+L I A D+G Y + + G N+ C I+ AS L D G ++ +E +L + + + ED P +P + E G A F V+V P+L EW
Sbjct: 20 PQILGPLSGGGEVPEGEPVHLELRIAPPGD--LQVQWFKDGVALSAGSRYNTMCERGLASLDVIYTIPEDSGTYFCVISNPYGHAQSEAVPVNVIPEID-PSQDHIDLQSADLYQATSAPTKEYER-DQRSQPKSAPHFTQQPVISSSDVLEGEPVRIEAYLDTTSDSTLQVDWMKNGQPVGTGSRFNAVLDRGLIVLEIGYCLAEDSGLYVCVATNALGHTESQPVELRCQPEERIVTKSILDQQS--INHLKQLE-EPGEDYDYMINSTVQGIPPSFKANLEPSSVQVSEDEPVIFSVPVDCGNGDRLVIEWKRDGQVVKTGSRITGRLELGIASLKIHYAQPNDTGAYTCHVSTEYGSADCGPSNLLCEATGSIIAASQLPGDKEKGLLAIEAIEANLHAPRGTVW---EDDSPHEAPVIIQQPQLVGEIEEGTAIHFDVQVEPAADPSLIVEW 466 HSP 4 Score: 92.4337 bits (228), Expect = 2.413e-15 Identity = 65/241 (26.97%), Postives = 119/241 (49.38%), Query Frame = 3
Query: 243 DHLYDSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPE-----EMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNG--DYSCVARNSLGKAETQS 944
D +++P Q + ++EG + +V PA D SL + WFK G LL++G+RF ++RG +LD+LYTI +D+G+Y C ++ +G +SN V NP + V + L + +++ I +D+ + Y +E+++ + P F + P T+ +G P+ + + L W+ + ++ S + D L++ C P G +A+N LGKAE ++
Sbjct: 424 DSPHEAPVIIQQPQLVGEIEEGTAIHFDVQVEPAADPSLIVEWFKSGNLLTSGTRFKVAYERGLAILDLLYTIPEDSGEYWCRVENKAGQVESNHVQVLCNPSASVITHSNLLQGSEGYNLIKAIEEVDQP-DGDEYRYVEDEE--VDSAPNFDVK-PQPATIGEGSPVKFLVRVSGKPTPQLT--WYLNDEPIEQDSITKIYSDGAINYLEMNRC-PALQGTNKLHVIAQNQLGKAEAET 657
BLAST of Titin vs. TrEMBL
Match: A0A095CAB5 (Titin OS=Schistosoma haematobium OX=6185 GN=MS3_07693 PE=4 SV=1) HSP 1 Score: 602.053 bits (1551), Expect = 0.000e+0 Identity = 314/735 (42.72%), Postives = 459/735 (62.45%), Query Frame = 3
Query: 297 VDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPE--SGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQ-LPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQ-----SKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEE-DLASGNISTFQNTEDVQEECLPNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLVVPDSVCKTYNDGSINYLEINRCSEL-GDIKVHVAATNPLGVAKEEATLTVIPMVDFRPDLRHITPENPFKKMAGLKKVECTSELNDAFKKSKPTAERIVKIEMGSETKSKFYKSPDVIETEKLYDQVAAGLRKN--IGPVSNNG 2465
V EGEP+ L R+ P G L++ WFKDG LSAGSR++T+ +RG LD++YTI +D+G Y CV + G AQS V + PE D++ ++ Q + K YE + + K+ P F Q + +S +++G+P+ IE +L SDS+L +W KDG + GSR + + DRG L+I +C ED+G Y CVA N+LG E+Q + C PEE I+TKS L + SI+ LK LE PGE+Y +++V +PPSF ++P + VSE P F V +D GNG +L +W +G++VKT SR+TG+LE+ I++L I Y P+DTG+Y C + +E+G + P L + SII Q KG+ AI A+EA L+AP+ + ED+ + P I+Q + + ++ EG ++ V+VEPA D SL VEW+K+ N + +G+R +++Y+RG A L+++ I EDSG+YW NK GQ +S + + C ++T S+LL S GY +K +EE D G+ ++ ED + + PNFDVKP T GEG P KFLV+VSG PTP L W ++D + DS+ K Y+DG+INYLE++RC L G K+HV A N LG A+ E LTV+ DFRPDL+H+ PENP+KKM GL+KV+C+ ELN A ++KP+A+ I+++E GSE K++ Y+SP+VIE EK+ DQ+A L+K+ P+ N G
Sbjct: 32 VPEGEPVHLELRIAPP--GDLQVQWFKDGVALSAGSRYNTMCERGLASLDVIYTIPEDSGTYFCVISNPYGHAQSEAVPVNVIPEIDPSQDHIDVQSADLYQATSAPTKEYE-------RDQRSQPKSAPHFTQQPVISSSDVLEGEPVRIEAYLNTTSDSTLQVDWMKDGQPVGTGSRFNAVLDRGLIVLEIGYCLAEDSGLYVCVATNALGHTESQPVELRCQPEERIVTKSILDQQSINHLKQLEEPGEDYDYMINSTVQGIPPSFKANLEPSSVEVSEDEPVIFSVPVDCGNGDRLVIEWKRDGQVVKTGSRITGRLELGIASLKIHYAQPNDTGAYTCHVSTEYGSADCGPSNLLCEATGSIIAASQLPGDKEKGLLAIEAIEANLHAPRGTVWEDDSPHEAPVIIQQPQLVGEIEEGTAIHFDVQVEPAADPSLIVEWFKSGNLLTSGTRFKVAYERGLAILDLLYTIPEDSGEYWCRVENKAGQVESNHVQVLCNPSASVITHSNLLQGSEGYNLIKAIEEVDQPDGD--EYRYIEDEEVDSAPNFDVKPQPATIGEGSPVKFLVRVSGKPTPQLTWYLNDEPIEQDSITKIYSDGAINYLEMSRCPALQGTNKLHVIAQNQLGKAEAETILTVLLAEDFRPDLKHVKPENPYKKMVGLRKVDCSPELNKALTRTKPSAQTIMEMERGSEMKARMYRSPEVIEAEKMLDQLALNLKKSEVKRPLVNGG 755 HSP 2 Score: 193.356 bits (490), Expect = 1.251e-47 Identity = 149/517 (28.82%), Postives = 250/517 (48.36%), Query Frame = 3
Query: 258 SPTF-QQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQL-PNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNL---TESSISQLKSLEN--PGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSII----LDPQSKGISAIHALEAELNAPKAE---MKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAIS-EDSGDYWLIATNKNGQTQSEKI 1763
+P F QQ + S V EGEP+R+ + D +L+++W KDG+ + GSRF+ + DRG IVL+I Y + +D+G YVCVA + G +S +R PE + + L + Q IN+L + E G + + P F + L P+S+ + + +P+ + ++ + LV EW +DG +K GSRI + G +L I + P D G Y+C G A+ N+ C SII S L E + ++++E + D S P +P EI EG +F V ++P L +W+ +G ++ + +R E ++ L++LYT+P D+G Y C + ++ G+VES V + S+I L S+G + I A+E E++ P + EDE + P+F + +P + EG V V+V + + + WY N+ I S +I D L + + + + +IA N+ G+ ++E I
Sbjct: 151 APHFTQQPVISSSDVLEGEPVRIEAYLNTTSDSTLQVDWMKDGQPVGTGSRFNAVLDRGLIVLEIGYCLAEDSGLYVCVATNALGHTESQPVELRCQPEERIVTKSILDQ---QSINHLKQLEEPGEDYDYMINSTVQGIPPSFKANLEPSSVEVSEDEPVIFSVPVDCGNGDRLVIEWKRDGQVVKTGSRITGRLELGIASLKIHYAQPNDTGAYTCHVSTEYGSADCGPSNLLCEATGSIIAASQLPGDKEKGLLAIEAIEANLHAPRGTVWEDDSPHEAPVIIQQPQLVGEI--EEGTAIHFDVQVEPAADPSLIVEWFKSGNLLTSGTRFKVAYERGLAILDLLYTIPEDSGEYWCRVENKAGQVESNHVQVLCNPSASVITHSNLLQGSEGYNLIKAIE-EVDQPDGDEYRYIEDEEVDSAPNFDVKPQPA-TIGEGSPVKFLVRV--SGKPTPQLTWYLNDEPIEQDSITKIYSDGAINYLEMSRCPALQGTNKLHVIAQNQLGKAEAETI 658 HSP 3 Score: 160.614 bits (405), Expect = 7.126e-37 Identity = 134/457 (29.32%), Postives = 211/457 (46.17%), Query Frame = 3
Query: 678 PKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSI----ILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPID-QLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADS-AGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAGEGGPA-KFLVKVSGFPTPTL--EW 2021
P+ L L + +G+P+H+E+ + D L +WFKDG++L GSR + + +RG +LD+ + PED+G Y CV N G A++++ V PE ++ ++ S ++ P +EY R + S P F + V EG P + ++ + L DW +G+ V T SR L+ + L I Y L D+G Y+CV + G ES+PV L Q E+ I ILD QS I+ + LE E M + PSF LEP +++E + V +V V+ N + L +EW ++ + GSR+ + G A+L I A D+G Y + + G N+ C I+ AS L D G ++ +E +L + + + ED P +P + E G A F V+V P+L EW
Sbjct: 20 PQILGPLSGGGEVPEGEPVHLELRIAPPGD--LQVQWFKDGVALSAGSRYNTMCERGLASLDVIYTIPEDSGTYFCVISNPYGHAQSEAVPVNVIPEID-PSQDHIDVQSADLYQATSAPTKEYER-DQRSQPKSAPHFTQQPVISSSDVLEGEPVRIEAYLNTTSDSTLQVDWMKDGQPVGTGSRFNAVLDRGLIVLEIGYCLAEDSGLYVCVATNALGHTESQPVELRCQPEERIVTKSILDQQS--INHLKQLE-EPGEDYDYMINSTVQGIPPSFKANLEPSSVEVSEDEPVIFSVPVDCGNGDRLVIEWKRDGQVVKTGSRITGRLELGIASLKIHYAQPNDTGAYTCHVSTEYGSADCGPSNLLCEATGSIIAASQLPGDKEKGLLAIEAIEANLHAPRGTVW---EDDSPHEAPVIIQQPQLVGEIEEGTAIHFDVQVEPAADPSLIVEW 466 HSP 4 Score: 93.5893 bits (231), Expect = 9.437e-16 Identity = 65/241 (26.97%), Postives = 120/241 (49.79%), Query Frame = 3
Query: 243 DHLYDSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPE-----EMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNG--DYSCVARNSLGKAETQS 944
D +++P Q + ++EG + +V PA D SL + WFK G LL++G+RF ++RG +LD+LYTI +D+G+Y C ++ +G +SN V NP + V + L + +++ I +D+ + Y +E+++ + P F + P T+ +G P+ + + L W+ + ++ S + D L+++ C P G +A+N LGKAE ++
Sbjct: 424 DSPHEAPVIIQQPQLVGEIEEGTAIHFDVQVEPAADPSLIVEWFKSGNLLTSGTRFKVAYERGLAILDLLYTIPEDSGEYWCRVENKAGQVESNHVQVLCNPSASVITHSNLLQGSEGYNLIKAIEEVDQP-DGDEYRYIEDEE--VDSAPNFDVK-PQPATIGEGSPVKFLVRVSGKPTPQLT--WYLNDEPIEQDSITKIYSDGAINYLEMSRC-PALQGTNKLHVIAQNQLGKAEAET 657
BLAST of Titin vs. TrEMBL
Match: G4V6G9 (Putative titin OS=Schistosoma mansoni OX=6183 GN=Smp_105020 PE=4 SV=1) HSP 1 Score: 597.045 bits (1538), Expect = 0.000e+0 Identity = 311/737 (42.20%), Postives = 457/737 (62.01%), Query Frame = 3
Query: 297 VDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPE--SGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQ-LPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQ-----SKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEE-DLASGNISTFQNTEDVQEECLPNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLVVPDSVCKTYNDGSINYLEINRCSEL-GDIKVHVAATNPLGVAKEEATLTVIPMVDFRPDLRHITPENPFKKMAGLKKVECTSELNDAFKKSKPTAERIVKIEMGSETKSKFYKSPDVIETEKLYDQVAAGLRKN--IGPVSNNGAR 2471
V EGEP+ L R+ P G L++ WFKDG LSAGSR++T+ +RG LD++YTI +D+G Y CV + G AQS V + PE D++ ++ Q + K YE + + K+ P+F Q + +S +++G+P+ IE +L SDS+L +W K+G + GSR + + DRG L+I +C ED+G Y CVA N+LG E+Q + C PEE I+TKS L + SI+ LK LE PGE+Y +++V +PPSF ++P + VSE F V +D GNG ++ +W +G++VKT SR+TG LE+ I++L I Y P+DTG+Y C + +E+G + P L + SII Q KG+ AI A+EA L+AP+ + EDE + P ++Q + + ++ EG ++ V+VEPA D SL VEW+K+ N + +G+R +++Y+RG A L+++ I EDSG+YW NK GQ +S + + C ++T S+LL S GY +K +EE D G+ ++ ED + + PNFDVKP T EG P KFLV+VSG PTP L W ++D + DS+ K Y+DG+INYLE+NRC L G K+HV A N LG A+ E LTV+ DFRPDL+H+ PENP+KKM GL+KV+C+ ELN A ++KP+A+ I+++E GSE K++ Y+SP+VIE EK+ DQ+A L+K+ P+ N G
Sbjct: 32 VPEGEPVHLELRIAPP--GDLQVQWFKDGVALSAGSRYNTMCERGLASLDVIYTIPEDSGTYFCVISNPYGHAQSEAVPVNVIPEIDPSQDHMDVQSADLYQATSAPTKEYE-------RDQRSQPKSAPQFTQQPVISSSDVLEGEPVRIEAYLNTTSDSTLQVDWMKNGQPVGTGSRFNAVLDRGLIVLEIGYCLAEDSGLYVCVATNALGHTESQPVELRCQPEERIVTKSILDQQSINHLKQLEEPGEDYDYMTNSTVQGIPPSFKANLEPSSVQVSEDESVIFSVPVDCGNGDRVVIEWKRDGQVVKTGSRITGSLELGIASLKIHYAQPNDTGAYTCHISTEYGSADCGPSNLLCEATGSIIAASQLPGDKEKGLLAIEAIEANLHAPRGTVWEDESPHEAPVILQQPQFVGEIEEGTAIHFDVQVEPAADPSLIVEWFKSGNLLTSGTRFKVAYERGLAILDLLYTIPEDSGEYWCRVENKAGQVESNHVQVLCNPSASVITHSNLLQGSEGYNLIKAIEEVDQLDGD--EYRYIEDEEVDSAPNFDVKPQPATIAEGSPVKFLVRVSGKPTPQLTWYLNDEPIEQDSITKIYSDGAINYLEMNRCPALQGTNKLHVIAENQLGKAEAETILTVLLAEDFRPDLKHVKPENPYKKMVGLRKVDCSPELNKALTRTKPSAQTIMEMERGSEMKARMYRSPEVIEAEKMLDQLALNLKKSEVKRPLVNGGGH 757 HSP 2 Score: 189.119 bits (479), Expect = 3.211e-46 Identity = 145/521 (27.83%), Postives = 248/521 (47.60%), Query Frame = 3
Query: 258 SPTF-QQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQL-PNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNL----------TESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSII----LDPQSKGISAIHALEA--ELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAIS-EDSGDYWLIATNKNGQTQSEKI 1763
+P F QQ + S V EGEP+R+ + D +L+++W K+G+ + GSRF+ + DRG IVL+I Y + +D+G YVCVA + G +S +R PE + + L + Q IN+L + E G + + P F + L P+S+ + + + + + ++ + +V EW +DG +K GSRI + G +L I + P D G Y+C G A+ N+ C SII S L E+ + L + E ++ VI P F+ I+ EG +F V ++P L +W+ +G ++ + +R E ++ L++LYT+P D+G Y C + ++ G+VES V + S+I L S+G + I A+E +L+ + EDE + P+F + +P + EG V V+V + + + WY N+ I S +I D L + + + + +IA N+ G+ ++E I
Sbjct: 151 APQFTQQPVISSSDVLEGEPVRIEAYLNTTSDSTLQVDWMKNGQPVGTGSRFNAVLDRGLIVLEIGYCLAEDSGLYVCVATNALGHTESQPVELRCQPEERIVTKSILDQ---QSINHLKQLEEPGEDYDYMTNSTVQGIPPSFKANLEPSSVQVSEDESVIFSVPVDCGNGDRVVIEWKRDGQVVKTGSRITGSLELGIASLKIHYAQPNDTGAYTCHISTEYGSADCGPSNLLCEATGSIIAASQLPGDKEKGLLAIEAIEANLHAPRGTVWEDESPHEAPVILQQPQFVGEIE-------EGTAIHFDVQVEPAADPSLIVEWFKSGNLLTSGTRFKVAYERGLAILDLLYTIPEDSGEYWCRVENKAGQVESNHVQVLCNPSASVITHSNLLQGSEGYNLIKAIEEVDQLDGDEYRYIEDEEVDSAPNFDVKPQPA-TIAEGSPVKFLVRV--SGKPTPQLTWYLNDEPIEQDSITKIYSDGAINYLEMNRCPALQGTNKLHVIAENQLGKAEAETI 658 HSP 3 Score: 167.548 bits (423), Expect = 4.150e-39 Identity = 137/457 (29.98%), Postives = 213/457 (46.61%), Query Frame = 3
Query: 678 PKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSI----ILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPID-QLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADS-AGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAGEGGPA-KFLVKVSGFPTPTL--EW 2021
P+ L L + +G+P+H+E+ + D L +WFKDG++L GSR + + +RG +LD+ + PED+G Y CV N G A++++ V PE ++ ++ S ++ P +EY R + S P F + V EG P + ++ + L DW NG+ V T SR L+ + L I Y L D+G Y+CV + G ES+PV L Q E+ I ILD QS I+ + LE E M + PSF LEP Q++E +SV +V V+ N + + +EW ++ + GSR+ S + G A+L I A D+G Y + + G N+ C I+ AS L D G ++ +E +L + + + ED P +P + E G A F V+V P+L EW
Sbjct: 20 PQILGPLSGGGEVPEGEPVHLELRIAPPGD--LQVQWFKDGVALSAGSRYNTMCERGLASLDVIYTIPEDSGTYFCVISNPYGHAQSEAVPVNVIPEID-PSQDHMDVQSADLYQATSAPTKEYER-DQRSQPKSAPQFTQQPVISSSDVLEGEPVRIEAYLNTTSDSTLQVDWMKNGQPVGTGSRFNAVLDRGLIVLEIGYCLAEDSGLYVCVATNALGHTESQPVELRCQPEERIVTKSILDQQS--INHLKQLE-EPGEDYDYMTNSTVQGIPPSFKANLEPSSVQVSEDESVIFSVPVDCGNGDRVVIEWKRDGQVVKTGSRITGSLELGIASLKIHYAQPNDTGAYTCHISTEYGSADCGPSNLLCEATGSIIAASQLPGDKEKGLLAIEAIEANLHAPRGTVW---EDESPHEAPVILQQPQFVGEIEEGTAIHFDVQVEPAADPSLIVEW 466 HSP 4 Score: 94.7449 bits (234), Expect = 4.453e-16 Identity = 65/241 (26.97%), Postives = 118/241 (48.96%), Query Frame = 3
Query: 252 YDSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPE--------EMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNG--DYSCVARNSLGKAETQS 944
+++P Q + ++EG + +V PA D SL + WFK G LL++G+RF ++RG +LD+LYTI +D+G+Y C ++ +G +SN V NP + V + L + + ++E++ LD + Y +E+++ + P F + P T+ +G P+ + + L W+ + ++ S + D L++ C P G +A N LGKAE ++
Sbjct: 427 HEAPVILQQPQFVGEIEEGTAIHFDVQVEPAADPSLIVEWFKSGNLLTSGTRFKVAYERGLAILDLLYTIPEDSGEYWCRVENKAGQVESNHVQVLCNPSASVITHSNLLQGSEGYNLIKAIEEVDQLDGD----EYRYIEDEE--VDSAPNFDVK-PQPATIAEGSPVKFLVRVSGKPTPQLT--WYLNDEPIEQDSITKIYSDGAINYLEMNRC-PALQGTNKLHVIAENQLGKAEAET 657
BLAST of Titin vs. TrEMBL
Match: Q5DI56 (SJCHGC09287 protein OS=Schistosoma japonicum OX=6182 GN=EWB00_003386 PE=2 SV=1) HSP 1 Score: 592.038 bits (1525), Expect = 0.000e+0 Identity = 305/725 (42.07%), Postives = 455/725 (62.76%), Query Frame = 3
Query: 297 VDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPE--SGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLP--NSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQ-----SKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLVVPDSVCKTYNDGSINYLEINRCSEL-GDIKVHVAATNPLGVAKEEATLTVIPMVDFRPDLRHITPENPFKKMAGLKKVECTSELNDAFKKSKPTAERIVKIEMGSETKSKFYKSPDVIETEKLYDQVAAGLRKN 2441
V EGEP+ L R+ P G L++ WFKDG LSAGSR++T+ +RG LD++YTI++D+G Y CV + G AQS V + PE D+L ++ Q + K YE + + K+ P+F SQ P +S +++G+P+ +E +L DS+L +W K+G + GSR + I DRG L+I +C +D+G Y CVA N+LG+ E+Q + C PEE I+T S L + SI+ LK LE PGE++ + ++ + PSF ++P + V+E P F V +D GNG + +W +G++VKT SR+TG LE+ I++L I Y P+DTG+Y C + +E+G + P L + SII Q KG+ AI A+EA L+AP+ + EDE + P I+Q + + ++ EG ++ V+VEPA+D SL VEW+K+ N + +G+R +++YDRG ATL+I+ I EDSG+YW NK GQ +S + + C ++T S+LL + GY +K +EE + + ++ ED + + P+FDVKP T GEG P KFLV+VSG PTP L W ++D + DS+ K Y+DG+INYLE++RC L G K+HV A N LG A+ E LTV+ DFRPDL+H+ PENP+KKM GL+KV+C+ ELN A ++KP+A+ I+++E GSE K++ Y+SP+V+E EK+ DQ+A+ LRK+
Sbjct: 32 VPEGEPVHLELRIDPP--GDLQVQWFKDGVALSAGSRYNTMCERGLASLDVIYTIQEDSGTYFCVISNPYGHAQSEAVPVNVIPEIDPNQDHLDAQFADLYQATSAPIKEYE-------RDQRSQPKSAPQF-SQQPVISSSDVLEGEPVRMEAYLNTTGDSTLQVDWMKNGQPVGTGSRFNAILDRGLVVLEIGYCLVDDSGLYVCVATNALGRTESQPVELRCQPEERIVTTSILDQQSINHLKQLEEPGEDFDHSANATIQGISPSFKANLEPATVQVNEDEPVIFSVPVDCGNGDHVVIEWKRDGQVVKTGSRITGSLELGIASLKIHYAQPNDTGAYTCHISTEYGSADCGPSNLLCEATGSIIAASQLPGDKEKGLLAIEAIEANLHAPRGTVWEDESPHEAPVIIQQPQSVGEIEEGTAIHFDVQVEPASDPSLIVEWFKSGNLLTSGTRFKVAYDRGLATLDILYTIPEDSGEYWCRVENKAGQMESNHVQVLCNASASVITHSNLLQGTEGYNLIKAIEE-VEQIDGDEYRYIEDEEVDSAPHFDVKPQAATIGEGSPVKFLVRVSGKPTPQLTWYLNDEPIEQDSITKIYSDGAINYLEMSRCHALQGTNKLHVIAQNQLGKAEAETVLTVVLAEDFRPDLKHVKPENPYKKMVGLRKVDCSPELNKALTRTKPSAQTILEMERGSEMKARTYRSPEVVEAEKMLDQLASSLRKS 745 HSP 2 Score: 185.267 bits (469), Expect = 5.111e-45 Identity = 145/522 (27.78%), Postives = 251/522 (48.08%), Query Frame = 3
Query: 258 SPTF-QQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTI-----PKFLSQL-PNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNL---TESSISQLKSLEN--PGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSII----LDPQSKGISAIHALE--AELNAPKAEMKEDEIITDLPSFIKQLEP-IDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAIS-EDSGDYWLIATNKNGQTQSEKI 1763
+P F QQ + S V EGEP+R+ + D +L+++W K+G+ + GSRF+ I DRG +VL+I Y + DD+G YVCVA + G +S +R PE + + L + Q IN+L + E G E+ H+A P F + L P ++ + + +P+ + ++ + +V EW +DG +K GSRI + G +L I + P D G Y+C G A+ N+ C SII S L E + ++++E + D S P +P EI EG +F V ++P + L +W+ +G ++ + +R + ++ L+ILYT+P D+G Y C + ++ G++ES V + S+I L ++G + I A+E +++ + EDE + P F ++P + EG V V+V + + + WY N+ I S +I D L + + + + +IA N+ G+ ++E +
Sbjct: 151 APQFSQQPVISSSDVLEGEPVRMEAYLNTTGDSTLQVDWMKNGQPVGTGSRFNAILDRGLVVLEIGYCLVDDSGLYVCVATNALGRTESQPVELRCQPEERIVTTSILDQ---QSINHLKQLEEPG-----EDFDHSANATIQGISPSFKANLEPATVQVNEDEPVIFSVPVDCGNGDHVVIEWKRDGQVVKTGSRITGSLELGIASLKIHYAQPNDTGAYTCHISTEYGSADCGPSNLLCEATGSIIAASQLPGDKEKGLLAIEAIEANLHAPRGTVWEDESPHEAPVIIQQPQSVGEI--EEGTAIHFDVQVEPASDPSLIVEWFKSGNLLTSGTRFKVAYDRGLATLDILYTIPEDSGEYWCRVENKAGQMESNHVQVLCNASASVITHSNLLQGTEGYNLIKAIEEVEQIDGDEYRYIEDEEVDSAPHF--DVKPQAATIGEGSPVKFLVRV--SGKPTPQLTWYLNDEPIEQDSITKIYSDGAINYLEMSRCHALQGTNKLHVIAQNQLGKAEAETV 658 HSP 3 Score: 166.392 bits (420), Expect = 9.166e-39 Identity = 141/485 (29.07%), Postives = 223/485 (45.98%), Query Frame = 3
Query: 678 PKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQL-KSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSI----ILDPQSKGISAIHALEAELNAPKAEMKEDEIITDL-PSFIKQLEPID-QLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADS-AGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAGEGGPA-KFLVKVSGFPTPTL--EWRIDDVLVVPDSVCKTYNDGSINYLEI 2099
P+ LS L + +G+P+H+E+ ++ D L +WFKDG++L GSR + + +RG +LD+ + ED+G Y CV N G A++++ V PE I + ++ + L ++ P +EY R + P +P+ V EG P + ++ L DW NG+ V T SR L+ + L I Y L D+G Y+CV + GR ES+PV L Q E+ I ILD QS H + E + + I + PSF LEP Q+NE + V +V V+ N + + +EW ++ + GSR+ S + G A+L I A D+G Y + + G N+ C I+ AS L D G ++ +E +L + + + ED P +P ++ E G A F V+V P+L EW L+ + K D + L+I
Sbjct: 20 PQILSPLCGGGEVPEGEPVHLELRIDPPGD--LQVQWFKDGVALSAGSRYNTMCERGLASLDVIYTIQEDSGTYFCVISNPYGHAQSEAVPVNVIPE--IDPNQDHLDAQFADLYQATSAPIKEYERDQRSQPKSAPQFSQQPVISSS-DVLEGEPVRMEAYLNTTGDSTLQVDWMKNGQPVGTGSRFNAILDRGLVVLEIGYCLVDDSGLYVCVATNALGRTESQPVELRCQPEERIVTTSILDQQSIN----HLKQLEEPGEDFDHSANATIQGISPSFKANLEPATVQVNEDEPVIFSVPVDCGNGDHVVIEWKRDGQVVKTGSRITGSLELGIASLKIHYAQPNDTGAYTCHISTEYGSADCGPSNLLCEATGSIIAASQLPGDKEKGLLAIEAIEANLHAPRGTVW---EDESPHEAPVIIQQPQSVGEIEEGTAIHFDVQVEPASDPSLIVEWFKSGNLLTSGTRFKVAYDRGLATLDI 492 HSP 4 Score: 96.6709 bits (239), Expect = 9.947e-17 Identity = 64/240 (26.67%), Postives = 119/240 (49.58%), Query Frame = 3
Query: 252 YDSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPE--------EMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYP-EDNGDYSCVARNSLGKAETQS 944
+++P Q +S ++EG + +V PA D SL + WFK G LL++G+RF +DRG LDILYTI +D+G+Y C ++ +G +SN V N + V + L + + ++E+ +D + Y +E+++ + P F + P + T+ +G P+ + + L W+ + ++ S + D L+++ C+ + +A+N LGKAE ++
Sbjct: 427 HEAPVIIQQPQSVGEIEEGTAIHFDVQVEPASDPSLIVEWFKSGNLLTSGTRFKVAYDRGLATLDILYTIPEDSGEYWCRVENKAGQMESNHVQVLCNASASVITHSNLLQGTEGYNLIKAIEEVEQIDGD----EYRYIEDEE--VDSAPHFDVK-PQAATIGEGSPVKFLVRVSGKPTPQLT--WYLNDEPIEQDSITKIYSDGAINYLEMSRCHALQGTNKLHVIAQNQLGKAEAET 657
BLAST of Titin vs. TrEMBL
Match: A0A504YQ44 (Titin OS=Fasciola gigantica OX=46835 GN=FGIG_06536 PE=4 SV=1) HSP 1 Score: 580.482 bits (1495), Expect = 0.000e+0 Identity = 298/739 (40.32%), Postives = 447/739 (60.49%), Query Frame = 3
Query: 252 YDSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPE--SGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQ-LPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQ-----SKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLVVPDSVCKTYNDGSINYLEINRCSEL-GDIKVHVAATNPLGVAKEEATLTVIPMVDFRPDLRHITPENPFKKMAGLKKVECTSELNDAFKKSKPTAERIVKIEMGSETKSKFYKSPDVIETEKLYDQVAAGLRKN 2441
+ P LE V EGEP+ L RV P G L++ WF+DG LSAGSRF+T +RG + LD++YT+ +D G Y CV + G + + V + E + D + + + + + YE + ++ K+ P+F Q + +S +++G+P+ IE L SD +L EW +DG + GSR + + DRG L+I +C ED+G Y CVA N+LG+ E+ + C PEE ++TKS L ++SI+ L+ LE PGEE++ Y +PP F PI+P + V+E P F V +D GNG +L+ +W + +IVKT SR+TG +E+ I++L I YT P+D G Y C + +E G+ +S+P +L + +II Q +G+ AI A+EA L+AP+ E++ P ++ L+PI ++ EG +V V VEP+ND SL V WYK+++ + G+R +++ DRG ATL++V I EDSG+YW N GQT S+++ + C I+T S+L+ S GY +K +EE A ++ E+ + + PNFDVKP T EG P +FLV+VSG PTP L W ++D +V PDS+ K Y DG+INYLE+ RC L G + V A NPLG A+ E L+V D+RPDL+H+ PENPFKKM L+KV+CT ELN A ++KP+A+ I ++E G+E K++ Y+SP+V+E E++ DQ+A LRK+
Sbjct: 17 HTEPQILSSLEGITEVPEGEPVHLELRVSPP--GDLQVQWFRDGHPLSAGSRFNTNVERGIVSLDVIYTLPEDGGMYSCVIANPFGMVKCDPVMVNVLAEVDAAQDQMDAQWADRYEATSAPIREYE-------RDQRNQPKSPPQFAQQPVISSSDVLEGEPIRIEAQLVPTSDVTLHVEWLRDGQPVGTGSRFNAVLDRGLVVLEIGYCLAEDSGHYVCVASNALGREESHPVELRCIPEERVVTKSILDQASINHLRQLEEPGEEHSYYTGDEKQGIPPQFKGPIEPASVQVTEDEPVIFSVPVDGGNGDRLTVEWRRDNQIVKTGSRITGMVELGIASLKIHYTQPTDAGVYTCHIETEFGQADSQPASLQCEVSGTIIAASQLPGDKERGLQAIEAIEALLHAPRGAAFEEDGPLGAPQILQDLQPIGEVEEGTAVHFEVHVEPSNDPSLIVYWYKDDDLLTTGTRFRVTNDRGVATLDLVYTIPEDSGEYWCKVQNAVGQTDSQRVPMKCLPSAAIITQSNLMQGSEGYNLIKAIEE-AAETVGGEYRYQEEEEPDSQPNFDVKPQAATISEGSPVRFLVRVSGKPTPRLTWYLNDQIVEPDSLTKIYTDGAINYLEMVRCPALQGSNALRVVAENPLGRAEAETVLSVTLAEDYRPDLKHVQPENPFKKMMVLRKVDCTPELNKALTRTKPSAQAIAEMERGTEMKARNYRSPEVLEAERMLDQLALNLRKS 745 HSP 2 Score: 159.844 bits (403), Expect = 1.371e-36 Identity = 137/486 (28.19%), Postives = 214/486 (44.03%), Query Frame = 3
Query: 666 AKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSI----ILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPID-QLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADS-AGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAGEGGPAKFLVKV--SGFPTPTLEWRIDDVLVVPDSVCKTYNDGSINYLEI 2099
A T P+ LS L + +G+P+H+E+ + D L +WF+DG L GSR + +RG +LD+ + PED G YSCV N G + V E + + + ++ P EY R + + PP F + V EG P + + P + L +W +G+ V T SR L+ + L I Y L D+G Y+CV + GR ES PV L E+ + ILD S I+ + LE E + DE P F +EP Q+ E + V +V V+ N + L VEW ++ + GSR+ + G A+L I D+G Y + GQ S+ ++ C I+ AS L D G Q ++ +E L + + F+ + + D++P+ EG F V V S P+ + W DD L+ + + ND + L++
Sbjct: 16 AHTEPQILSSLEGITEVPEGEPVHLELRVSPPGD--LQVQWFRDGHPLSAGSRFNTNVERGIVSLDVIYTLPEDGGMYSCVIANPFGMVKCDPVMVNVLAEVD-AAQDQMDAQWADRYEATSAPIREYER-DQRNQPKSPPQFAQQPVISSSDVLEGEPIRIEAQLVPTSDVTLHVEWLRDGQPVGTGSRFNAVLDRGLVVLEIGYCLAEDSGHYVCVASNALGREESHPVELRCIPEERVVTKSILDQAS--INHLRQLE-EPGEEHSYYTGDEKQGIPPQFKGPIEPASVQVTEDEPVIFSVPVDGGNGDRLTVEWRRDNQIVKTGSRITGMVELGIASLKIHYTQPTDAGVYTCHIETEFGQADSQPASLQCEVSGTIIAASQLPGDKERGLQAIEAIEALLHAPRGAAFEEDGPLGAPQILQ-DLQPIG-EVEEGTAVHFEVHVEPSNDPSLIVYWYKDDDLLTTGTRFRVTNDRGVATLDL 492
BLAST of Titin vs. Ensembl Cavefish
Match: ENSAMXT00000044348.1 (pep primary_assembly:Astyanax_mexicanus-2.0:19:23245281:23271894:-1 gene:ENSAMXG00000032661.1 transcript:ENSAMXT00000044348.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 87.4261 bits (215), Expect = 5.441e-18 Identity = 68/250 (27.20%), Postives = 115/250 (46.00%), Query Frame = 3
Query: 1494 PSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLTVIPMVDFRPDLRHITPENP 2243
P F K+L + +L + V ++ P D ++ VEW + + +R+++ + G +L+ +A + DSG ATNK G Q+ + EG+ +V + L + QR+ +++ G + E+V E+ P + P + EG AKF +V+G+P P + W ++ LV+ + DG I+YLE+ C ++ V A NP GV + V DFR LR P
Sbjct: 23 PHFKKKLTSV-RLKQFGPVHFECRLTPIGDPTMIVEWLHDGKPLEAANRLRMVNEFGYCSLDYEAAYARDSGVITCRATNKFGIDQTSA-TLLVREGKGLVEETQL-PEGRSAQRIDEIKRLAHEGPSGV--SGEEVTEKIKPEIVLLPEPVRVLEGETAKFRCRVTGYPAPKVNWYLNGQLVLKSKRFRLLFDG-IHYLEVVDCKSYDAGEMKVLAENPEGVIEHTVKFEVQQKEDFRTVLRQTKGPAP 266 HSP 2 Score: 72.4034 bits (176), Expect = 4.812e-13 Identity = 86/368 (23.37%), Postives = 145/368 (39.40%), Query Frame = 3
Query: 678 PKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTES----SISQLKSL--ENP----GEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYD-RGTATLNIVSAISEDSGDYWLIATNKNGQT 1748
P F +L S+ L Q P+H E L D +++ EW DG L+ +R+ ++ + GYC+LD Y D+G +C A N G +T S + + ++ ++ L E I ++K L E P GEE ++ +P EP + V EG A F+ + K+ +WY NG++V R + I L ++ D G + + G +E + K E+++ E D + ++Q + G + +V S V+W K+ HI G + + G+ L I++ DSG Y A+N G+
Sbjct: 23 PHFKKKL-TSVRLKQFGPVHFECRLTPIGDPTMIVEWLHDGKPLEAANRLRMVNEFGYCSLDYEAAYARDSGVITCRATNKFGIDQT-SATLLVREGKGLVEETQLPEGRSAQRIDEIKRLAHEGPSGVSGEEVTEKIKPEIVLLP----EP-----VRVLEGETAKFRCRVTGYPAPKV--NWYLNGQLVLKSKRFRLLFD-GIHYLEVVDCKSYDAGEMKVLAENPEGVIE---------------------------------HTVKFEVQQKE---DFRTVLRQTKGPAPCTVGGNAVFEYRVAGVPLPS--VQWLKDSCHIQPGKFCNMVNNVDGSGFLKIINVQKNDSGLYSCRASNLRGEA 338 HSP 3 Score: 60.8474 bits (146), Expect = 2.302e-9 Identity = 54/226 (23.89%), Postives = 99/226 (43.81%), Query Frame = 3
Query: 261 PTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPE-EMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAE 935
P F++ L S + G P+ R+ P D ++ + W DGK L A +R + + G+ LD D+G C A + G Q++ A + + G+ TQLPE Q I+ + + G G+ ++ K P+ + LP + +++G+ + + + W+ +G + R +++D G L++ C D G+ +A N G E
Sbjct: 23 PHFKKKLTSVRLKQFG-PVHFECRLTPIGDPTMIVEWLHDGKPLEAANRLRMVNEFGYCSLDYEAAYARDSGVITCRATNKFGIDQTS-ATLLVREGKGLVEETQLPEGRSAQRIDEIKRLAHEGP-SGVSGEEVTEKIKPEIV-LLPEPVRVLEGETAKFRCRVTGYPAPKV--NWYLNGQLVLKSKRFRLLFD-GIHYLEVVDCKSYDAGEMKVLAENPEGVIE 241
BLAST of Titin vs. Ensembl Cavefish
Match: ENSAMXT00000031302.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02002274.1:904514:909134:1 gene:ENSAMXG00000031537.1 transcript:ENSAMXT00000031302.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 63.929 bits (154), Expect = 1.705e-10 Identity = 68/283 (24.03%), Postives = 122/283 (43.11%), Query Frame = 3
Query: 255 DSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSL--EINWFKDGKLLSA----GSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESII----TKSNLTESSISQLKSLENPGEEYARYNDTSV 1073
+PTF Q L+S +V EG +V GS E++WF+DG++L+A G++ S F G VL I +G + A + +G A S A + + E+ P F+ +L SLT+ QG + +++ + + V +++++G ++ + ++ + +L IA +PED+G YS A NS G+A T + + EE + TK+ ++ + ISQ + AR+ T++
Sbjct: 4 QAPTFTQPLQS-VVALEGSAATFEAQV----SGSPVPEVSWFRDGQVLTAAALPGAQIS--FSDGRAVLRIPAVTAAHSGRFSVRATNGAGQATST-AELLVTAETAP---------------------------------------PTFIQRL-QSLTVRQGSRVRLDVRVT--GIPTPVVKFYREGAEIQSSADFQILQEGDLYSLLIAEAFPEDSGTYSVNATNSSGRA-TSTAELLVQGEEEAVPAKKTKTVISTTQISQARQTRVEKRMEARFESTAM 235
BLAST of Titin vs. Ensembl Cavefish
Match: mylk5 (myosin light chain kinase, smooth muscle-like [Source:NCBI gene;Acc:103036671]) HSP 1 Score: 62.7734 bits (151), Expect = 1.449e-9 Identity = 61/246 (24.80%), Postives = 105/246 (42.68%), Query Frame = 3
Query: 1086 PSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGK--LEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTL---------------NVQTEKSIILDPQSKGISAIHALEAE----LNAPKAEMKED-EIITDLPSFIKQLEPIDQLNE--GDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQ 1751
P F+EP+ ++ V EG+ + I Q + W+HNGE++ + G+ L + L I LP D G+Y CV + G+ S ++Q ++ QS+G +++ EA + P+ +E+ I + ++ + P DQ+ G+ V L+ E + W +N +++G R I +TL I A EDSG Y L+ ++N TQ
Sbjct: 28 PVFLEPL--QDSIVDEGSDITLRGVIT--GKQPIRVSWFHNGEVM-----MFGESSLVRGEATLVIKECLPEDAGAYTCVAENTAGKTSSSSAVCVKGNKHTQTDIHTQTHIQCTTQLLTSLQSRGCASLILTEASSVCCVKDPQQPQRENSHIYVNFFIPLQVISPRDQVKVRLGEGVRLSC--EFKGSYPVVSCWIRNGKEVLDGGRYSIVSSSDRSTLVISDAQPEDSGRYTLVVRDRNSSTQ 262 HSP 2 Score: 52.373 bits (124), Expect = 2.419e-6 Identity = 55/205 (26.83%), Postives = 83/205 (40.49%), Query Frame = 3
Query: 786 WFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTC-----HPEESIITKSN--------------------LTE-SSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVL 1322
WF +G + G V RG L I C PED G Y+CVA N+ GK T S + C H + I T+++ LTE SS+ +K + P E + IP+ I P D ++ + EG +++ + + + W NG+ V R + + S L I P D+G Y V+
Sbjct: 61 WFHNGEVMMFGESSLV---RGEATLVIKECLPEDAGAYTCVAENTAGK--TSSSSAVCVKGNKHTQTDIHTQTHIQCTTQLLTSLQSRGCASLILTEASSVCCVKDPQQPQRENSHIYVNFFIPL--QVISPRDQVKVRLGEGV----RLSCEFKGSYPVVSCWIRNGKEVLDGGRYSIVSSSDRSTLVISDAQPEDSGRYTLVV 254
BLAST of Titin vs. Ensembl Cavefish
Match: obsl1b (obscurin like cytoskeletal adaptor 1b [Source:ZFIN;Acc:ZDB-GENE-050809-29]) HSP 1 Score: 63.1586 bits (152), Expect = 1.544e-9 Identity = 59/225 (26.22%), Postives = 90/225 (40.00%), Query Frame = 3
Query: 321 LMTRVGPADDGSL----------EINWFKDGKLLSA---GSRFSTIF-DRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQ--LPNS-----LTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDR-GYCALDIAFCYPEDNGDYSCVARNSLGK 929
L TRVG +D E+ W KDGK L+ + FS + D G+ L I T D G Y C A++ G + + A + ++ G E Y G + G + + + I L LPNS + +G+ ++ ++ W KDG + G R + DR GY L + +C +DNG Y C A N+ G+
Sbjct: 119 LSTRVGRGEDAVFSCKLWGNPRPEVAWEKDGKKLNEIFESTHFSVGYQDGGWFQLKIFKTRAPDGGVYTCKARNEFGESLAG-AVLLVDAGPG------------HEDEGNRNGYTNGHWKGHQGKPRSGRPIAARLKDEPLPNSAKVKMFAVTEGKHAKFRCYVTGKPKPEII--WRKDGRLILSGRRYLLYEDREGYFTLKVLYCKQQDNGVYVCAASNTAGQ 328 HSP 2 Score: 56.9954 bits (136), Expect = 1.006e-7 Identity = 47/193 (24.35%), Postives = 84/193 (43.52%), Query Frame = 3
Query: 360 EINWFKDGKLLSAGSRFSTIFDR-GFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINP--------------ESGVDYL-TQLPEEMMQEINNL-DKNYETGTYHGMEEDKHAAKTIPK---------FLSQLPNS---------LTLIQGQPLHIEI-------FLEHHSDSSLVTEWFKDGMSLK 812
EI W KDG+L+ +G R+ DR G+ L +LY + DNG YVC A + +G S + H+ + P E + L ++PE+ + L D+ + G +GMEE + + +L ++P+ T+++ P +++ F + + W+KDG+ L+
Sbjct: 277 EIIWRKDGRLILSGRRYLLYEDREGYFTLKVLYCKQQDNGVYVCAASNTAGQTLSAV-HLSVKPPVRFKQPLTDLQVWERDLAILECEVPEDSVPITWYLEDRRLQPGAKYGMEEWGTKRRLTIRDIGVDDDGIYLCEMPDGGRSIAEVAVKTIVRKLPRKVDVLEGENAAFCVEVEEEEMDIHWYKDGVELR 468
BLAST of Titin vs. Ensembl Cavefish
Match: OBSCN (obscurin, cytoskeletal calmodulin and titin-interacting RhoGEF [Source:HGNC Symbol;Acc:HGNC:15719]) HSP 1 Score: 61.6178 bits (148), Expect = 4.181e-9 Identity = 61/212 (28.77%), Postives = 97/212 (45.75%), Query Frame = 3
Query: 363 INWFKDGKLLSAGSRFSTIFDR-GFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQL-PEEMMQEIN-----NLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESI 977
+ WFKDGK+L + D+ G L I D G Y CVA++ SG A S A +R+ DY T E ++ + ++ +T + + ED+ +P+ + +L + L L G P + ++ + + WFKDG L+ RI V +RG +L+IA ED G+YS N+ G A + + V P E +
Sbjct: 4724 VRWFKDGKMLQEDDHYMINEDQEGCHQLIITAVRPVDMGVYRCVAENDSGIASSK-AELRVEMSCSSDYDTAADATETSSYVSAKGYVSSSEHRDTEAFESVVEDEQ----LPQVVDEL-HDLFLSPGAP-NAKMSVRVKGFPAPKVYWFKDGSPLQPSKRILVSSERGVYSLEIAEVTREDTGEYSAYISNAAGSAYSSARMVILGPGERM 4928 HSP 2 Score: 51.9878 bits (123), Expect = 3.745e-6 Identity = 70/322 (21.74%), Postives = 125/322 (38.82%), Query Frame = 3
Query: 1077 PMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSR-LTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDE----IITD--LPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEW 2021
P P F+ ++ +E V +G P F I S W+ +G++++ + + + L I P D G Y CV ++ G + S L V+ S D + + A+ +E ++ E ++ D LP + +L + + ++V+V+ +Y W+K+ + + R+ +S +RG +L I ED+G+Y +N G S + GE++ Y + +D +E L P F + N T +G VKV G P PT+ W
Sbjct: 4690 PAAPVFLTQLNSQE--VPDGYPVSFDCVII--GKPPPSVRWFKDGKMLQEDDHYMINEDQEGCHQLIITAVRPVDMGVYRCVAENDSG-IASSKAELRVEMSCSSDYDTAADATETSSYVSAKGYVSSSEHRDTEAFESVVEDEQLPQVVDELHDLFLSPGAPNAKMSVRVKGFPAPKVY--WFKDGSPLQPSKRILVSSERGVYSLEIAEVTREDTGEYSAYISNAAGSAYSSARMVILGPGERM----------PDYGK-RDSKEPLKP-----------------PRFLERFCNRTVKQGASITLSVKVEGSPAPTINW 4976 HSP 3 Score: 51.2174 bits (121), Expect = 5.664e-6 Identity = 89/372 (23.92%), Postives = 149/372 (40.05%), Query Frame = 3
Query: 678 PKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYC-ALDIAFCYPEDNGDYSCVARNSLGKAETQS---CNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIP--MPPSFIEPIDPREISVSEGAP-AYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVE--PANDNSLYVEWYKNE--NHII----NGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQT 1748
P FL+QL NS + G P+ + + S+ WFKDG L+ + D+ C L I P D G Y CVA N G A +++ ++C + + T S +S K + E SV+ P ++ + ++ +S GAP A V + K+ W+ +G ++ R+ E + +L I DTG Y + + G S + + +IL P + P ++ + P F+++ + +G S+TL+VKVE PA + W K E ++ + +++ +L ++ E SG Y IATN GQT
Sbjct: 4693 PVFLTQL-NSQEVPDGYPVSFDCVIIGKPPPSV--RWFKDGKMLQEDDHYMINEDQEGCHQLIITAVRPVDMGVYRCVAENDSGIASSKAELRVEMSCSSDYDTAADATETSSYVSA-KGYVSSSEHRDTEAFESVVEDEQLPQVVDEL--HDLFLSPGAPNAKMSVRVKGFPAPKVY--WFKDGSPLQPSKRILVSSERGVYSLEIAEVTREDTGEYSAYISNAAGSAYS--------SARMVILGPGER-------------MPDYGKRDSKEPLKPPRFLERF-CNRTVKQGASITLSVKVEGSPAPT----INWLKEEPMKEVLWIKADTPGYKVASSGCLHSLILMDVGKEHSGTYTCIATNSAGQT 5030
BLAST of Titin vs. Ensembl Sea Lamprey
Match: ENSPMAT00000002502.1 (pep scaffold:Pmarinus_7.0:GL476400:249000:366561:-1 gene:ENSPMAG00000002253.1 transcript:ENSPMAT00000002502.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 104.375 bits (259), Expect = 7.238e-23 Identity = 141/552 (25.54%), Postives = 229/552 (41.49%), Query Frame = 3
Query: 678 PKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSL--KLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSN-DWYHNGEIVKTCSRLTGKLE--MNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGIS-AIHALEAELNAPKAEM-----------------KEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLTVIPMVDFRPDLRHITPENPFKKMAGL 2264
P F+ Q PNS+ + +GQ ++ + V WF +G + +I VI + G +L I P D G+++ +A N GK +SI+ ++ + + E +R P F+E + R +SV EGAP V G + + W N EI+ + ++E SAL+I T D+ Y ++ GR ++ +NV+ E + IS I+ L+ E+ AP+ E KE E P F K+L + +L + V ++ P D ++ VEW + + +R ++ + G +L+ A DSG ATNK G Q+ I E + +V S L G QR++++E G I + +TE E+ P + P EG A++ +V+G+P P + W ++ L+ + DG I YL+I C +V + A NP G A+ TV V R D R + + P K GL
Sbjct: 857 PVFV-QKPNSVQVPEGQTARFDLKVVGRPMPDTV--WFHNGHQVVNDYTHKI-VIKEDGTQSLIIIPAMPADTGEWTVIAENRAGK------------------------TSIT-VQLIVDAKEHLSR----------PHFVEKL--RNVSVKEGAPVQLSVK---AAGNPIPDIVWLKNSEIISSQKFPNMRIEGATGESALSIDTTTQHDSAWYTSTAINKAGR-DTTRCKVNVEAEAFEPEPERKMVISKGIYKLK-EVTAPELEPLHLRYGQEQWEEGDLYDKEKE---QKPLFKKKLTSV-RLKQFGPVHFECRLTPIGDPTMVVEWLHDSKPLDAANRFRMVNEFGYCSLDYEVAYPRDSGIITCRATNKYGADQTSATLIVKDE-KSLVEESQLPEGRRGIQRIQEMERQALEGGIVSV-STEGTLEKVKPEIVLFPEPARVLEGETARYRCRVTGYPQPRVSWYLNGQLIHKSKRYRLRYDG-IYYLDIVDCKSYDSGEVKLLAENPEGKAEH----TVKIEVQLREDFRTLLRKAPEPKPEGL 1351 HSP 2 Score: 79.337 bits (194), Expect = 3.165e-15 Identity = 95/401 (23.69%), Postives = 174/401 (43.39%), Query Frame = 3
Query: 237 GSDHLYDSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFI---VLDILYTIEDDNGDYVCVAKSHSGT----AQSNIAHV--------RINPESGVDYLTQL--PEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTES--SISQLKSLENPGEE---YARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQ 1373
+HL P F + L + + V EG P++L V A + +I W K+ +++S+ +F + G L I T + D+ Y A + +G + N+ ++ G+ L ++ PE + + +E G + E+++ P F +L S+ L Q P+H E L D ++V EW D L +R ++ + GYC+LD YP D+G +C A N G A+ S + E+S++ +S L E I +++ +E E + + ++ + P + + P V EG A ++ + ++S WY NG+++ R + + I L+I+ D+G + + G+ E V + VQ
Sbjct: 949 AKEHL-SRPHFVEKLRN-VSVKEGAPVQL--SVKAAGNPIPDIVWLKNSEIISS-QKFPNMRIEGATGESALSIDTTTQHDSAWYTSTAINKAGRDTTRCKVNVEAEAFEPEPERKMVISKGIYKLKEVTAPELEPLHLRYGQEQWEEGDLYDKEKEQK-----PLFKKKL-TSVRLKQFGPVHFECRLTPIGDPTMVVEWLHDSKPLDAANRFRMVNEFGYCSLDYEVAYPRDSGIITCRATNKYG-ADQTSATLIVKDEKSLVEESQLPEGRRGIQRIQEMERQALEGGIVSVSTEGTLEKVKPEIV--LFPEPARVLEGETARYRCRVTGYPQPRVS--WYLNGQLIHKSKRYRLRYD-GIYYLDIVDCKSYDSGEVKLLAENPEGKAE-HTVKIEVQ 1331 HSP 3 Score: 75.0998 bits (183), Expect = 6.878e-14 Identity = 122/535 (22.80%), Postives = 188/535 (35.14%), Query Frame = 3
Query: 159 NGTTMAPHALEVVNQVNKRSLASPRRGSD------HLYDSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGT-AQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNG 1742
N +A + V +Q A+ GSD + D P+F + L+ + G PLRL V DD + + W K GK + + S F VL+IL + D GDYVC A S +G+ + ++ V+ +P L L E+ + K L L SL +G P+ + WFK+ +K R+ + + L+I ED+ DY+C A+N +G T SC TC S+ ++ PSF +P++ E V + +V +D W N + + KLE + L I +D G Y C + + G+ ES HA +L P P F K+ +++EG S TL + + + W K++ I ISY A L IV A +G+Y A N+ G
Sbjct: 4210 NQCILAIGNVAVAHQGTYACKATNEFGSDTCSAQLSVTDKPSFSKALQP-VKAAVGTPLRLECEVD--DDKGVTVAWTKSGKEIRPSRKLSFTFKNKLAVLEILKSELADTGDYVCTASSEAGSESTASSVEVKADPAG----LRALQPEVKE---------------------------AKGLVHLDCSL---KGSPV-------------IKVTWFKNDNEVKADGRVQLSFVNNVAVLEIRDSETEDSADYTCEAQNEVG---TVSC--TC-------------AVSVKEI----------------------PSFKKPLESME--VRGNSDVTLEVELD--GSPPFDVKWVKNARELPKIDKYRAKLENTTATLRIGNFSAADVGQYSCKVKNNVGKDES-------------------------HA-NIKLKEP-------------PKFTKKFIN-SEVSEGSSATLNATL--IGSQPMTIAWSKDKLKIAEDENHSISYKDNHAILTIVKAEKSHAGEYTCDAKNEAG 4608 HSP 4 Score: 68.1662 bits (165), Expect = 9.107e-12 Identity = 120/551 (21.78%), Postives = 196/551 (35.57%), Query Frame = 3
Query: 249 LYDSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVC----VAKSHSGTAQSNIAHVRIN-------------------------PESGVDYLTQ--LPEEMMQ------------EINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQL--PNSLT------LIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYF--KVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNG 1742
+ + P+F + +ES+ VV G + + V + L + WF+D L +G R F G L + G Y C A S + +A + R N PE V + Q L EE + E+ N+DK+ E G Y +++ + L+ L PN + ++ G +H+E + + W+K LK G + + + L++ D GDYSC A N G A ++L S+S+ K+ PP F + ++ EG A F +VT P S WY +G + + E N++ L+I + G Y C +F+ D S SA K E K+ ++ PSF K+ +P+ ++ G+S L + A + + W KN I + ++ + A L ++ A EDSG Y A N+ G
Sbjct: 5307 VIEPPSFTKTVESQHVVP-GASVEFKSSVSGTE--PLTVKWFRDDNELMSGRRCKITFLNGVATLKLDDVDAASAGVYSCQVSNAAASETCSANLTVKAERANFRRRVKDSQVSNGETAVFSTSFSGTPEIVVFWYKQNNLIEESDKYRVAISEGESTLEVLNVDKSDE-GIYTCKIQNEAGEDSCLATLATLEPPNFIKGLEPTEIVVGSTVHLE--CQVAGTPQIRVSWYKGSQKLKSGDKCKMAFKNNLATLELRSLTKADTGDYSCKAENDFGSA------------------TSLASLSVSERKN-------------------PPYFTRKL--KDWQAVEGTAATFDCRVTGTP----PFSFIWYKHGVELSSGEDYVISAEENVATLDIHNVKLTHAGEYTCRVFN----------------------DVDSASCSA-----------KLEAKDKKV---PPSFEKKPDPV-EVTAGESFELECLI--AGSRPIRISWLKNNREIRSVGNYKLFFYDNAARLTVLKAGKEDSGTYVCKAVNEVG 5769 HSP 5 Score: 64.3142 bits (155), Expect = 1.349e-10 Identity = 57/231 (24.68%), Postives = 97/231 (41.99%), Query Frame = 3
Query: 252 YDSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLG--SRIDVIYDR--GYCALDIAFCYPEDNGDYSCVARNSLGKA 932
Y + T ++ + V EGE +R+ +V A + I WF++ + + F F G L I +D+G + C A + +GT ++ YL EE + L+ + +GT G I + Q P L++G + ++ + W K G+ L G R V Y++ G C+L+I+ + +D G+Y+CV RN G A
Sbjct: 373 YGAATVVKEGLKDTTVVEGESVRMECQV--AGFPTPAIMWFREEYRIERSADFQIAFQAGVASLTIREAFAEDSGRFTCTATNEAGTVSTS------------SYLLVQGEESI----FLEDSEASGTDTG-------GDGIAPYFVQKPQPQHLVEGGSVVFHCQIKGSPKPHVY--WKKSGIPLTTGYRRRYKVHYNKETGECSLEISMTFSDDAGEYTCVVRNKQGDA 576 HSP 6 Score: 63.5438 bits (153), Expect = 2.355e-10 Identity = 110/555 (19.82%), Postives = 177/555 (31.89%), Query Frame = 3
Query: 357 LEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEW 2021
+ + WFK + ++ G R+ F L+I + D Y C A + SG+ S I P S + E +H +P + +S++ +PL ++ WF+D L G R + + G L + G YSC N+ +ET S N+T E + + R D+ V S G A F + ++ WY +++ + + S L +L SD G Y C + +E G + ++ + LE P+FIK LEP ++ G +V L +V A + V WYK + +G + ++++ ATL + S D+GDY A N G S LAS ++S +N P F K + A EG A F +V+G P + W
Sbjct: 5247 ISVVWFKGSEEIAQGERYKMTFVENVATLEIQKVVASDAFSYTCKATNVSGSEASTADLKVIEPPS---------------------------FTKTVESQHV---VPGASVEFKSSVS--GTEPLTVK--------------WFRDDNELMSGRRCKITFLNGVATLKLDDVDAASAGVYSCQVSNA-AASETCSANLTVKAERANFRR----------------------RVKDSQV------------------SNGETAVFSTSF--SGTPEIVVFWYKQNNLIEESDKYRVAISEGESTLEVLNVDKSDEGIYTCKIQNEAG---------------------EDSCLATLATLEP------------------PNFIKGLEPT-EIVVGSTVHLECQV--AGTPQIRVSWYKGSQKLKSGDKCKMAFKNNLATLELRSLTKADTGDYSCKAENDFGSATS-----------------------------------LASLSVSERKNP--------PYFTRKLKDWQAVEGTAATFDCRVTGTPPFSFIW 5627 HSP 7 Score: 58.151 bits (139), Expect = 1.018e-8 Identity = 128/616 (20.78%), Postives = 219/616 (35.55%), Query Frame = 3
Query: 357 LEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSG---TAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGT--YH-----GMEE----DKHA--------------------------------------------AKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTE--WFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSE-KINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEW 2021
+ + W+K + LS G+++ T+F VL++ + D G Y C A++ G T+ S R +P S L + ++++ + D TGT +H G E DKH KT P F +L + + G P LE H SL + W KD ++ G + + L I D+G+Y+C A N +G + +C E++I++ K + P F + P EI + E K+ Q ++ W + + + + NI+ +++ T SD+G Y+C +E G + VTLN++ K HA P+F K+++ + + EGD+++ + +V + L + W K+ + ++ I+Y G TL I D+G+Y A N+ G S+ K+ E K ++ + + + G I F +P N E G KF KV G P PT++W
Sbjct: 6653 IRVTWYKGSQKLS-GAKYKTMFQHNTAVLEVKNFEKADVGTYSCEAENSLGSAKTSASLAGKERKHPPSFSRMLKNM-QQVIGDCAKFDARV-TGTPPFHVSWFLGDSELSYSDKHVISFEDNVASLSIFSLKVSDAGEYTCKVSNEDGSISCSAKLDVTERKTKPTFDQKL-EPVEAVVGDPAE----LECHLTGSLPIKIFWMKDKKDIRPGPNCKMTFFDNTPHLTIYKLDKSDSGEYTCKAVNDIGSDNSCTC-----------------ETAIAERK-------------------VAPMFTK--KPLEI-LDEEEGKGVKLDSRVSGSQPIAITWTKDDKDLHAHPGRELSFKNNIALMSLKSTKVSDSGLYVCKAENEAG-TATFKVTLNIKERK--------------HA---------------------PNFDKKVQSLTVV-EGDTLSASCRV--SGSEPLQIMWLKDRKEVQPFGKVSIAYSEGDCTLEISQVTKADAGEYTCKAVNEAGSDISKVKVTTKAPEAGKPARKTAAPVAAPTAAPAPVVVD----GKIF---------------FVEEPKNAKVEEKGTVKFTAKVGGEPIPTVKW 7163 HSP 8 Score: 55.0694 bits (131), Expect = 9.061e-8 Identity = 47/163 (28.83%), Postives = 70/163 (42.94%), Query Frame = 3
Query: 1536 EGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEWR 2024
EG+SV + +V A + + W++ E I + QI++ G A+L I A +EDSG + ATN+ G + T+S LL L+D E ASG T+ + P F KP EGG F ++ G P P + W+
Sbjct: 390 EGESVRMECQV--AGFPTPAIMWFREEYRIERSADFQIAFQAGVASLTIREAFAEDSGRFTCTATNEAGT---------------VSTSSYLLVQGEESIFLEDSE---ASG-------TDTGGDGIAPYFVQKPQPQHLVEGGSVVFHCQIKGSPKPHVYWK 525 HSP 9 Score: 55.0694 bits (131), Expect = 9.138e-8 Identity = 57/234 (24.36%), Postives = 91/234 (38.89%), Query Frame = 3
Query: 1494 PSFIKQLEPIDQLNEGDSVTLTVKVE--PANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLTV 2189
PSFIK L+ + L EG + T ++ P + V W+++ I + ++IS+ G ATL I + + SG Y L ATN +GQ S E +VTA + PNF + +I+ +G + V+V+G P T+++ + + + DG + L I V A+N +G A A L V
Sbjct: 6 PSFIKPLQSVVAL-EGSAATFEAEISGLPVPE----VSWFRDGQAISSAPGVEISFSSGRATLTISAVSAAQSGKYSLRATNGSGQATS--------TAELLVTAQT-----------------------------------APPNFTQRLQSISVSQGKEVRLDVRVTGIPAATVKFFREGAEIHSSQDFRLVQDGDLYSLIIAEAYPQDSGTYSVTASNNVGRATSSAQLLV 191 HSP 10 Score: 51.9878 bits (123), Expect = 6.697e-7 Identity = 101/514 (19.65%), Postives = 165/514 (32.10%), Query Frame = 3
Query: 363 INWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSG---TAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQL-PNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGR-VESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQ 1889
+ WFK GK ++ G ++ F+ + L+IL +D +Y C + G A S R NP P F QL P S+T G+P + + W KD ++ G+ + L I D+G Y C A N G + +C P + E ++ P+F + ++ ++ E A VT + W + ++ NI+ L IL D+G Y C ++ G V S +T+ Q P PSF+++L+ D + T + A + V W KN I++G + + ++ TA+L I+ D G Y ATN G+ SC I+ S L +K +++ SG+ +TFQ
Sbjct: 6088 VAWFKHGKEITPGEKYRMSFEENVVFLEILNLATNDEEEYSCTVSNDVGKISCAASLEVKERKNP-------------------------------------------PSFDQQLAPASVT--AGEPADLGCHVT--GSPPFKISWLKDNKEIRPGTNYKFSFVDNTPLLTILKTDKRDSGVYVCRAANEAGY------DTSCAP-------VTIKERRVA------------------------PTFDQKLESLNVTTGERAELECHVT----GSVPIKVTWAKDNREIRAAGNYRMIFMENIARLTILKVGKLDSGIYTCAASNDVGSDVTSTEITVKDQA------------------------VP-------------PSFVRKLKKTDAILHS---TAQMDCRVAGTVPILVAWSKNGVEIVDGDKYRCLFEENTASLEILKVDKSDDGSYTCTATNVAGRD-------SCSTTLSIIEPPSFL--------MKMEPQEVLSGSRATFQ 6458 HSP 11 Score: 51.2174 bits (121), Expect = 1.419e-6 Identity = 57/231 (24.68%), Postives = 90/231 (38.96%), Query Frame = 3
Query: 258 SPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAA---KTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGM-SLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAET 938
+P F + ++S L V EG+ L RV ++ L+I W KD K + + S + G L+I + D G+Y C A + +G+ S + PE+G + + G +EE K+A K KF ++ + G+P+ +W K L G R+ V L+I D G Y CVA N G+ E+
Sbjct: 7008 APNFDKKVQS-LTVVEGDTLSASCRVSGSE--PLQIMWLKDRKEVQPFGKVSIAYSEGDCTLEISQVTKADAGEYTCKAVNEAGSDISKVKVTTKAPEAG-KPARKTAAPVAAPTAAPAPVVVDGKIFFVEEPKNAKVEEKGTVKFTAK-------VGGEPIP-------------TVKWIKGKWRQLNHGGRLTVQQKGADALLEIREATKTDAGPYRCVANNRHGEIES 7214 HSP 12 Score: 50.0618 bits (118), Expect = 2.605e-6 Identity = 49/213 (23.00%), Postives = 85/213 (39.91%), Query Frame = 3
Query: 711 TLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNI----SALNILYTLPSDTGSYICVLFSEHG 1337
T+++G+ + +E + +++ WF++ ++ + + + G +L I + ED+G ++C A N G T S + EESI L+ E G DT + P F++ P+ + EG F I K W +G + T R K+ N +L I T D G Y CV+ ++ G
Sbjct: 387 TVVEGESVRMECQVAGFPTPAIM--WFREEYRIERSADFQIAFQAGVASLTIREAFAEDSGRFTCTATNEAGTVSTSS-YLLVQGEESIF------------LEDSEASG------TDTGGDGIAPYFVQKPQPQHL--VEGGSVVFHCQIK--GSPKPHVYWKKSGIPLTTGYRRRYKVHYNKETGECSLEISMTFSDDAGEYTCVVRNKQG 574 HSP 13 Score: 50.0618 bits (118), Expect = 2.650e-6 Identity = 134/696 (19.25%), Postives = 226/696 (32.47%), Query Frame = 3
Query: 258 SPTFQQDLESELVVDEGEPLRLMTRVGPADDGSL--EINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATN--------KNGQTQSEKINI-------SCFEGEKIVTASSL------------------------LADSAGYQRLKDLEEDLASGNISTFQ-NTEDVQEECL--------PNF--DVKPMNITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLTV 2189
+PTF Q L+ + V G+P V GSL +I W K+ K + +G + F L ++ T + G Y C A + ETG+ H + + +TIP + SL + G E + + W KD ++ G + L I D G Y+C KA + + C + S+ + P+ PSF+ + + ++S A K++ +S W+ E + R N++ L I + SD SY C ++ +S A A+L + + PSF K +E + G SV V + L V+W++++N +++G R +I++ G ATL + + +G Y +N N ++E+ N GE V ++S +A S G L+ L D + I T + E ++ CL PNF ++P I G +V+G P + W + CK ++ LE+ ++ A N G A A+L+V
Sbjct: 5025 APTFDQKLQP-VEVTAGDPSEFECHV----TGSLPIKITWSKNNKEIRSGGNYRITFLDNTPRLIVMKTDRSEGGVYTCKATN-----------------------------------------ETGSDHCSTQLEVKERTIPPDFDEKLESLEVTAGLSADFECHVT--GSAPFRVTWTKDNRDIRSGGNYKFTFLDNTAHLTILKADKGDIGTYTC-------KAANAAGSAACTADLSVKDR------------------------------PVAPSFVRKLKTTDAALSSTAHLECKLS----GSVPISVVWFKGSEEIAQGERYKMTFVENVATLEIQKVVASDAFSYTC----------------------------KATNVSGSEASTADLK-----------VIEPPSFTKTVES-QHVVPGASVEFKSSV--SGTEPLTVKWFRDDNELMSGRRCKITFLNGVATLKLDDVDAASAGVYSCQVSNAAASETCSANLTVKAERANFRRRVKDSQVSNGETAVFSTSFSGTPEIVVFWYKQNNLIEESDKYRVAISEGESTLEVLNVDKSDEGIYTCKIQNEAGEDSCLATLATLEPPNFIKGLEPTEIVV--GSTVHLECQVAGTPQIRVSWYKGSQKLKSGDKCKMAFKNNLATLELRSLTKADTGDYSCKAENDFGSATSLASLSV 5587
BLAST of Titin vs. Ensembl Sea Lamprey
Match: ENSPMAT00000000826.1 (pep scaffold:Pmarinus_7.0:GL476876:251045:270583:1 gene:ENSPMAG00000000753.1 transcript:ENSPMAT00000000826.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 102.834 bits (255), Expect = 2.040e-22 Identity = 161/718 (22.42%), Postives = 275/718 (38.30%), Query Frame = 3
Query: 228 PRRGSDHLYDSPTFQQDLESELV---VDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTI--FDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLS--------QLPNSLTLIQGQPLHIEIFLEHHSDSSLV------------------TEWFKDGMS-LKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSND-----WYHNGEIVKTCSRLTGKLE--MNISALNILYTLPSDTGSYICVLFSEHGR------VESEPVTLNVQTE--------KSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLTVIPMVDFRPDLR 2222
PR D P+F+ +ES LV V EG P ++ + +I W+KDG+ + G + + +G + L I + +D G Y +A + SG A + +++ E + LP M + GT + A + LS L ++ I + LH +F++ +S+ T WF +G L + V+ + G +L + P D G+++ VA+N G + S E + + LE P F+E P + V EG+ + + N W N +I+ ++E S L I PSD Y + + GR V+ E + + Q E K + D + +H + + ++ + E P F K+L + +L + V ++ P D + VEW + + +R +I + G +L+ A DSG ATNK G Q+ I E + +V S L G+ RL++LE+ +A +S ++ D +E+ P F + P +G A++ +V+G P P + W ++ + + DG I+YLEI C +V + A N G + +A L ++P DFR LR
Sbjct: 15 PRHSED---TGPSFEPAVESRLVSQRVQEGFPAAFFCQL--SGHPLPKILWYKDGRRIFTGPHYQLVGPTRQGEVELHIPSILPEDEGIYTVLACNASGNA---VCSAKLSIEPAGNGAGGLPSPMSPARQQIPMPAWPGTQGSASQLPPPANRSQRPLSPERAEPVGALQDTSAEILVERLHKPMFVQKPESASVTEGQTARFDLRVVGRPMPDTFWFHNGKQVLNDQTHKVVVKEDGTQSLIVVPATPADAGEWTVVAQNKGG------------------STSVSVELVVEAKERLEKP-----------------RFVE--KPNNVCVKEGSAVQL-------TARAIGNPTPDIVWQRNNDIIFPHKFPHYEVENTKGESTLTIYSAKPSDMAWYTIIALNRAGRTMARCKVQVEAMLSDRQPEPERKLIIPKGVYRDIAPPVLEPLHLRHGQDQWNEGDLYDKET-QQRPFFKKKLTSV-RLKQFGPVHFECQMTPIGDPHMVVEWLHDGQPLQAANRFRIVCEFGYCSLDYEVAYPRDSGIITCRATNKFGCDQTSGSLIVKDE-KSLVEESQLPEGRRGFHRLQELEKGMAGTAVSK-EDVPDAREK--PYFVMYPEPCRVYDGETARYHCRVTGVPQPKVLWFLNGQRIHRSKRFRLRYDG-IHYLEIVDCKSYDTGEVKLVAENSEGSVECKAVLEILPKQDFRAVLR 673 HSP 2 Score: 65.0846 bits (157), Expect = 6.153e-11 Identity = 74/283 (26.15%), Postives = 118/283 (41.70%), Query Frame = 3
Query: 150 VIPNGT--TMAPHALEVVNQVNKRSLASPRRGSDH-----LYDS-----PTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPE--EMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTE----WFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQS 944
+IP G +AP LE ++ R G D LYD P F++ L S + G P+ ++ P D + + W DG+ L A +RF + + G+ LD D+G C A + G Q++ + + + +S V+ +QLPE + L+K GT +ED A+ P F+ P + G+ +H + V + WF +G + R + YD G L+I C D G+ VA NS G E ++
Sbjct: 397 IIPKGVYRDIAPPVLEPLHL---------RHGQDQWNEGDLYDKETQQRPFFKKKLTSVRLKQFG-PVHFECQMTPIGDPHMVVEWLHDGQPLQAANRFRIVCEFGYCSLDYEVAYPRDSGIITCRATNKFGCDQTSGSLIVKDEKSLVEE-SQLPEGRRGFHRLQELEKGM-AGTAVS-KEDVPDAREKPYFV-MYPEPCRVYDGETAR------YHCRVTGVPQPKVLWFLNGQRIHRSKRFRLRYD-GIHYLEIVDCKSYDTGEVKLVAENSEGSVECKA 658
BLAST of Titin vs. Ensembl Sea Lamprey
Match: ENSPMAT00000005850.1 (pep scaffold:Pmarinus_7.0:GL476948:68064:92656:1 gene:ENSPMAG00000005287.1 transcript:ENSPMAT00000005850.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 65.4698 bits (158), Expect = 4.298e-11 Identity = 60/207 (28.99%), Postives = 94/207 (45.41%), Query Frame = 3
Query: 1461 EMKEDEIITD---LPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGS-RMQISYD-RGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECL-PNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLVVPDSVCK 2063
E++ D+I T P F ++L+ EG +T + KV +Y W+K+ N I S R Q+ D G +L+I +D G+Y ++A N G+ ISC G +V + A Q + S + + +++ VQE+ P+F P + A EG + KVSG PTP L W ++ V PD+ K
Sbjct: 321 EVEHDDIPTGRCIAPIFERKLKHFKTF-EGSPITFSCKVSGTPTPKIY--WFKDGNQISKKSERFQMQRDSEGNCSLHIPLTTMDDDGNYTVMAGNPQGR-------ISC-SGRLMVQNVNQRGRVARSQSGQPAARRPRSQSRESRDDSDMVQEKNFRPHFLQAPGDTMAHEGKLCRLDCKVSGLPTPELAWEVNGRPVRPDATHK 516
BLAST of Titin vs. Ensembl Sea Lamprey
Match: mylka (myosin, light chain kinase a [Source:ZFIN;Acc:ZDB-GENE-030131-8207]) HSP 1 Score: 63.5438 bits (153), Expect = 2.206e-10 Identity = 85/372 (22.85%), Postives = 141/372 (37.90%), Query Frame = 3
Query: 258 SPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQ 1373
SP F +S V +G + RV ++E W KDG ++ R+S ++G L I + D G + C AK+ G A T T+ + + A P F + L + T+ + Q +E L + S+ W KD + S + D GYC L + +D G Y+C+A N LGKA S VT ++ N + ++ Q PP F++ + ++ V +G+ + V + ++ W HNG +K + + + L+I P DTG Y C ++E G+ +S+ + L VQ
Sbjct: 338 SPVFTHPPQSREVT-QGHEVAFSCRVSGIPKPAVE--WSKDGVVIKTSERWSVEENQGGHQLIIFDVQKYDGGAFACAAKNSDGKAT------------------------------------TATFTLIVKGVTDADQAPGFSAPL-VACTVEEQQEFTLECHLLGKPEPSVT--WLKDDKPVN--SAVMSCRD-GYCTLYVGAAGLDDGGVYTCIAENHLGKAYC-SATVTVREPRRRTSRRNEEKPAVKQ---------------------SPPVFVKGLC--DLQVMDGSEVHLTVKVAAIPPAEIV--WLHNGNEIKESEDFVLESKDDEHTLHIQEVFPEDTGCYTCEAWNEAGQAQSQAL-LTVQ 637 HSP 2 Score: 60.077 bits (144), Expect = 2.447e-9 Identity = 53/202 (26.24%), Postives = 79/202 (39.11%), Query Frame = 3
Query: 1596 VEWYKNENHIINGSRMQISYD-RGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGN---ISTFQNTEDVQEECLPNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLTV 2189
V W+KN + G ++ RG +L +V +DSG Y A N+ G Q I + D +RL +E+ A+ + I ++ + E P F KP EG KF K++G P P + WR D + D K Y S+++LE+ E TN G A A L +
Sbjct: 10 VLWFKNGQPLAAGEHFRVERSSRGAFSLEVVGLGEDDSGKYLCEARNEAGTRQ-------------ITVELKVEGDD---RRLTRRDENTAACSGFPIPPVESRPSIWGESPPKFVNKPSRAVVREGQGGKFNCKITGRPQPQVTWRKGDTELHNDGHFKVYEKSSLHFLEVLDVKEEDSGVYTCTITNSAGKASASAELII 195
BLAST of Titin vs. Ensembl Sea Lamprey
Match: ENSPMAT00000010530.1 (pep scaffold:Pmarinus_7.0:GL477073:104451:224074:1 gene:ENSPMAG00000009530.1 transcript:ENSPMAT00000010530.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 51.9878 bits (123), Expect = 6.977e-7 Identity = 32/86 (37.21%), Postives = 48/86 (55.81%), Query Frame = 3
Query: 270 QQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDR-GFIVLDILYTIEDDNGDYVCVAKSHSGTAQSN 524
+Q E EL+VD G+P+ L R P + S ++ WFKD KLL + SR R G +L ++ + D G Y C A +H G+A ++
Sbjct: 1632 EQPSEDELIVD-GQPMTLYCR--PVGNPSPDVKWFKDKKLLGSESRAKVTVSREGQHILSVVKAGKKDTGVYECSATNHLGSAATS 1714
BLAST of Titin vs. Ensembl Nematostella
Match: EDO49558 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RGM2]) HSP 1 Score: 87.8113 bits (216), Expect = 5.764e-18 Identity = 146/672 (21.73%), Postives = 244/672 (36.31%), Query Frame = 3
Query: 261 PTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSA--GSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSG--TAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYE-TGT-------YHG------------MEEDKHAAKTIPKFLSQLPNSLTLIQGQPLH---IEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVE--PANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLTV 2189
P F+ L ++ V+EG + L R+ E++WFKDGK +S RF L I D G Y+C A + +G T+ N+ + + P+ + L + + E + + + TG+ YH + +D+H++ + L T + + + + +E + V WF +G ++ R+ + D L I +D Y+C A N LG+ E S++ + G P F++ ++ +E V EG+ +V + K +W +GE+VK S +T + E + L I+ T D G Y C+ ++ G V +A +E + P+ +K PS + Q+ EG +V P + VEW ++ I G R QI +++ + L I+ A D G+Y +AT+K G+ SC + LL D A P F K NI EG A+F V+++G P P + W D+V + + Y+D + L I C+ ATN G A LT+
Sbjct: 3 PEFKSALR-DIEVNEGNTVTL--RIHVTGSPKPEVDWFKDGKPVSGLPHRRFEVGCVGEVYTLVIKEARGSDTGKYLCKAYNSAGEVTSSCNVDVLHVAPKEPPKFERNLKDITIAEGCSTRFDVQVTGSPEPKVFWYHNGKLVQPRDHVDIVHDDEHSSLILMHCPLDLSGKYTCVAENEVENDAVRLEVEVTGEPKPVVLWFCEGKPIEENRRVYTMRDGSTSVLVIENAELDDEAMYTCKAINDLGEVECS-----------------------SEMLPTDEAG---------------PKFLQRLELKE--VVEGSRTDLEVEV--SGKPKPVVEWLKDGELVKPSSLMTLETEGQVHTLTIMNTTLDDEGEYTCIAKNKVGEV----------------------SCTAELLVEEGMRPPQFIVK--------PSSL-------QIMEGKMAKFEARVSGLPGPE----VEWLRDGRPIKPGRRFQIEFEKDRSVLTIIDAKLNDEGEYTCVATSKAGKD-------SC--------SVELLVDEAVVP----------------------------PEFVRKISNIELTEGEIARFDVRITGTPAPEVTWYKDEVPIEDGGRFEIYSDDDKHSLVIRDCALSDTGSYSCKATNEAGQASCYGELTI 545
BLAST of Titin vs. Ensembl Nematostella
Match: EDO49553 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RGL7]) HSP 1 Score: 75.485 bits (184), Expect = 4.358e-14 Identity = 117/532 (21.99%), Postives = 191/532 (35.90%), Query Frame = 3
Query: 678 PKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVES-------EPVTLNVQTEKSIILDPQSK-------------GISAIHALEAELNAPK--------AEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLTV 2189
P F+S+L + + +G + + + +WF+D + +K R YD AL + +D+G Y CV N+ G S+ +K+++T LK P +YA I F+EP+ EI V + + V P K + W+ NG+ ++ + + L IL + D GSY C+ ++ G E EP + + I+ D +S+ I A + E A AEM ++ + P F+ +L P+ + EGD+V+ KV A VEWY++ + +R ++ +D +L + A +D G Y + TN G S +L+ D + + DVQ EG A+F V+ SG P P + W + + V +S DG L I + ATN G L V
Sbjct: 16 PTFMSKL-EPIEITEGDDFQLSCNVTGRPQPEV--QWFRDEVLIKPSRRFKASYDGNVSALTVKEAELDDDGVYKCVVTNNFG---------------SVSSKADVT-----VLKKHLRPERDYA------PIFEEKQFLEPL---EIQVRQPVEVSYHVRGRP----KPTVTWWKNGKELRDGKHVEIRSGGTYHTLYILRSEEEDAGSYKCLARNDVGSAERLFEVQVEEPSVTWYRNKNEIVPDGRSRTTFDGETYKLILQDIKIKDAGQYECIASNPIGKAFCTAEMTVNK--REAPVFVSKLRPL-FVTEGDNVSWMCKV--AGMPRPTVEWYRDNQPLRADTRWKMDFDGRVCSLFLSEADCKDKGVYNCVITNSAGSAFSS----------------------------AELQVDKKANKPKVIEKMNDVQ---------------TVEGTEARFDVRFSGLPRPEVTWYRNSIKVRNESRFHLSEDGDRYSLTITDTKLVDTGTYKCVATNEGGKITSRGDLDV 463
BLAST of Titin vs. Ensembl Nematostella
Match: EDO49554 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RGL8]) HSP 1 Score: 73.1738 bits (178), Expect = 2.240e-13 Identity = 99/413 (23.97%), Postives = 157/413 (38.01%), Query Frame = 3
Query: 1086 PSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQ----------TQSEKINISCFEGEKIV-TASSLLADSAGYQRLKDLEEDLAS-------------GNISTFQNTEDVQEECLPNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLVVPDSVCKTYNDGSINYLEIN--RCSELGDIKVHVAATNPLGVAKEEATLTV-----IPMVDFRPD-LRHI 2228
PS + P E++ KV+++P K + WY +VK R++ + S L+I D G YIC + +E G+ S+ EL K +K P + +++ +++ G+ V V+V VEWY+ H+ +G R IS +L + +DSG Y L+A N G+ + K I+ F V T + ++A G +L+E S G+ S+ +++ +P F V E + VK+ GFP PT+E+ D L+V K DG L I R + G K + TN LGV EA + V P D +P+ LR +
Sbjct: 15 PSLSSQLMPVEVTEGNDIDLVCKVSVNP----KPTVTWYKGDALVKLDDRISAHFDGRFSRLSIQKATKDDEGIYICDVQNELGKAFSK----------------------------VELTVRKRNVK--------PQIVGEIKDAEEVERGE-VRFEVRV--TGFPKPRVEWYRGPVHLTDGGRFSISEKEDMYSLTVRDLKRDDSGTYKLVAQNTVGKCDKYTLVCRVSGKPKPTITWFRDNTPVHTDNRVVASYEGSVSALELKEMKLSDAGLYKCTATNSQGSTSSSAEVTIKRKDAIPTFVVGLKETEGLESSDMRLDVKIGGFPRPTVEFYKDYDLIVDSERHKILEDGDRCSLIITNLRPEDAGTYKCVI--TNLLGVETSEAVIRVQEREHAPRFDVKPEFLRKV 382 HSP 2 Score: 61.6178 bits (148), Expect = 9.159e-10 Identity = 99/454 (21.81%), Postives = 156/454 (34.36%), Query Frame = 3
Query: 786 WFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIE----PI---DPREISVSEGAPAY--------------FKVTIDPGNGQ--------------KLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQE-ECLPNFDVKP------MNITAGEGGPAKFLVKVSGFPTPTLEW 2021
W+K +KL RI +D + L I +D G Y C +N LGKA ++ E + K N+ + ++K E R+ + V P +E P+ D S+SE Y +K+ G+ K + W+ + V T +R+ E ++SAL + SD G Y C + G S AE+ + + +P+F+ L+ + L D + L VK+ VE+YK+ + I++ R +I D +L I + ED+G Y + TN G SE + VQE E P FDVKP ++TA E F + + G+P P +EW
Sbjct: 48 WYKGDALVKLDDRISAHFDGRFSRLSIQKATKDDEGIYICDVQNELGKAFSKV--------ELTVRKRNVKPQIVGEIKDAEEVERGEVRF-EVRVTGFPKPRVEWYRGPVHLTDGGRFSISEKEDMYSLTVRDLKRDDSGTYKLVAQNTVGKCDKYTLVCRVSGKPKPTITWFRDNTPVHTDNRVVASYEGSVSALELKEMKLSDAGLYKCTATNSQGSTSS----------------------------SAEVTIKRKDA--------IPTFVVGLKETEGLESSD-MRLDVKI--GGFPRPTVEFYKDYDLIVDSERHKILEDGDRCSLIITNLRPEDAGTYKCVITNLLGVETSEAVI--------------------------------------------RVQEREHAPRFDVKPEFLRKVEDVTALETEQVWFEIPIQGYPRPIVEW 409
BLAST of Titin vs. Ensembl Nematostella
Match: EDO49557 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RGM1]) HSP 1 Score: 72.7886 bits (177), Expect = 2.658e-13 Identity = 126/539 (23.38%), Postives = 206/539 (38.22%), Query Frame = 3
Query: 678 PKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEP-IDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQ-----------TEKSIILDPQSKGISAIHALEAELNAPKAEMKEDE------IITDLPSFIKQLE-------------PIDQLNEGDSVTLTVKV--EPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQ-TQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLVVPDS-VCKTYNDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLTV 2189
P+F+ L + + +GQ E ++ + S+ EWFK+G+ LK RI Y+ +L I C +D G+Y CVARNS GKA SC ++T N T + PP FI+ + V+E +V + G K S W + E++ + ++ L I D G Y V+ S H + S +NV+ ++ ++ D K + P K DE I D+ S + + P + EG+ V L+VK+ +P + V W K+E + +++ + TL I A DSG Y TN G+ T++ + I EGE ++ K E + + ++EE LP + +TA +V+G P P++EW DDV V PD + T DG+ + L I ++ + V +N G+ + ++V
Sbjct: 1 PQFI-DLMQPVEVTEGQTATFECTVKGYPRPSI--EWFKNGLPLKENERIASTYEGALSSLTINQCTIDDEGNYKCVARNSFGKA---SCAT------ELLTNLNGTHT-------------------------HPPHFIDKHFETAPAEVTEEGDVRLEVRV--GGHPKPSVQWLKDDEVIISSPHFMVSSHADVHTLVINSPNQDDIGQY-KVIASNHRGMASRTFDVNVEGKPKPSVEWLKDDRPVLSDSHIKMTEEDDIYSLTIREP---TKSDEGMYTVKITNDVGSGTRTFDVKVEDTTTNDSFVPFESTEEGN-VRLSVKITGKPVPE----VSWTKDEKPVHKIQNIRLDHQDDIYTLTIREATPNDSGSYKCTLTNDAGKSTRTFSVEI---EGE------------TKPEKGKPTE-----------REGKPLEEEELPVSAETEVEMTA----------QVTGKPQPSVEWFKDDVPVSPDEHITITEEDGTYS-LVIKEPTKKDEGTYTVEVSNEAGLGRRTFDVSV 454
BLAST of Titin vs. Ensembl Nematostella
Match: EDO49550 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RGL4]) HSP 1 Score: 70.8626 bits (172), Expect = 3.270e-13 Identity = 79/303 (26.07%), Postives = 126/303 (41.58%), Query Frame = 3
Query: 1251 EMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHA-LEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVE--PANDNSLYVEWYKNENHIINGSRMQIS--YDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSE---KINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVH 2135
E + L I T P D G Y C+ + G V S+ L+V+ E +I PQ + + +L + I+ P F ++L + ++ EGD V+V+ P + VEWY N I++ R +I + G +L + + ED+G Y IA NK G++ ++N F E ++L QRL +P++ GE K +VSG P PT+ W D ++ + D+ KTY DG + L I R +LGD ++
Sbjct: 53 ENGLFVLTIRNTRPEDAGEYQCIAVNGAGEVTSKGY-LHVEEELTI---PQFIDMGTYKCQVRNDLGRTSTSAHLNIIVATRPEFKEKLTKV-EVTEGDRAKFEVRVKGYPYPE----VEWYHGSNRILDEDRFEIESVKETGYYSLTVGEVVQEDAGMYKCIAVNKAGKSTCRADLEVNERQFAPEFETEEPTIL------QRL-------------------------------QPVDTIEGEA--IKMECRVSGKPEPTITWLKDGLVFLTDNRIKTYFDGDVCTLTI-RDVKLGDEGIY 306 HSP 2 Score: 48.521 bits (114), Expect = 5.886e-6 Identity = 47/168 (27.98%), Postives = 70/168 (41.67%), Query Frame = 3
Query: 1539 GDSVTLTVKVE----PANDNSLYVEWYKNENHIINGSRMQI-SYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGY--QRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEW 2021
G+ V ++VE PA V +++ I +G R I + G L I + ED+G+Y IA N G+ S+ E+ +T + D Y Q DL S +++ T P F K + EG AKF V+V G+P P +EW
Sbjct: 15 GEDVRFEIQVEGTPVPA------VTFHRESEQIYDGGRYSIVEEENGLFVLTIRNTRPEDAGEYQCIAVNGAGEVTSK----GYLHVEEELTIPQFI-DMGTYKCQVRNDLGRTSTSAHLNIIVATR-------PEFKEKLTKVEVTEGDRAKFEVRVKGYPYPEVEW 164
BLAST of Titin vs. Ensembl Medaka
Match: ENSORLT00000046443.1 (pep primary_assembly:ASM223467v1:21:968974:990901:1 gene:ENSORLG00000030355.1 transcript:ENSORLT00000046443.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 79.7221 bits (195), Expect = 5.579e-16 Identity = 57/225 (25.33%), Postives = 98/225 (43.56%), Query Frame = 3
Query: 729 PLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTES-----SISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSI 1388
P H E L D ++V EW DG L +R+ ++ + GYC+LD Y D+G +C A N G +T S + E+ ++ ++ L E I +++ L + G Y + + P + + P + V EG A F+ + K+ +WY NG++++ N L +L P D Y C +++G S L VQ +++
Sbjct: 37 PAHFECRLTPIGDPTMVVEWLHDGKPLAAANRLRMVNEFGYCSLDYEVAYARDSGVITCRATNKFGVDQT-SATLIVKDEKGLVVETQLPEGRKGAHRIDEMERLAHEGGPYGVTAEEQTEKLKPEIV--LLPEPVRVVEGDTARFRCRVTGYPAPKV--NWYLNGQLIRK----------NEHTLMLLEVFPEDAAIYSCSAKNDYGEATSS-APLIVQGTRTL 245 HSP 2 Score: 67.0106 bits (162), Expect = 9.692e-12 Identity = 48/213 (22.54%), Postives = 94/213 (44.13%), Query Frame = 3
Query: 312 PLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPE--EMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQS 944
P R+ P D ++ + W DGK L+A +R + + G+ LD D+G C A + G Q++ A + + E G+ TQLPE + I+ +++ G +G+ ++ K P+ + LP + +++G + + + W+ +G ++ ++ + +PED YSC A+N G+A + +
Sbjct: 37 PAHFECRLTPIGDPTMVVEWLHDGKPLAAANRLRMVNEFGYCSLDYEVAYARDSGVITCRATNKFGVDQTS-ATLIVKDEKGLVVETQLPEGRKGAHRIDEMERLAHEGGPYGVTAEEQTEKLKPEIV-LLPEPVRVVEGDTARFRCRVTGYPAPKV--NWYLNGQLIRKNEHTLMLLE----------VFPEDAAIYSCSAKNDYGEATSSA 235 HSP 3 Score: 59.3066 bits (142), Expect = 3.265e-9 Identity = 42/161 (26.09%), Postives = 75/161 (46.58%), Query Frame = 3
Query: 1563 KVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEK-IVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLV 2042
++ P D ++ VEW + + +R+++ + G +L+ A + DSG ATNK G Q+ I + EK +V + L G R+ ++E LA E+ E+ P + P + EG A+F +V+G+P P + W ++ L+
Sbjct: 43 RLTPIGDPTMVVEWLHDGKPLAAANRLRMVNEFGYCSLDYEVAYARDSGVITCRATNKFGVDQTSATLI--VKDEKGLVVETQLPEGRKGAHRIDEMER-LAHEGGPYGVTAEEQTEKLKPEIVLLPEPVRVVEGDTARFRCRVTGYPAPKVNWYLNGQLI 200
BLAST of Titin vs. Ensembl Medaka
Match: ENSORLT00000027090.1 (pep primary_assembly:ASM223467v1:21:27247530:27253950:1 gene:ENSORLG00000024593.1 transcript:ENSORLT00000027090.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 77.0258 bits (188), Expect = 6.016e-15 Identity = 61/226 (26.99%), Postives = 101/226 (44.69%), Query Frame = 3
Query: 1563 KVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLTVIPMVDFRPDLRHITPEN 2240
++ P D ++ VEW + + +R+++ + G +L+ A + DSG A+NK G Q+ I E + +V + L G R+ ++E + G + E+ E+ P + P EG A+F +V+G+P P + W ++ L+ + DG I YLEI +V V A NPLG ++ L + DFR LR PE
Sbjct: 12 RLTPIGDPTMVVEWLHDSKPLAAANRLRMVNEFGYCSLDYEVAYARDSGVITCRASNKFGVDQTSATLIVKDE-KGLVEETQLPEGRKGAYRVDEIERMVHEGGPAGVSADEE-SEKTKPEIVLLPEPARVLEGDIARFRCRVTGYPAPKVNWYLNGQLIRKSKRYRLNYDG-IYYLEIVDTKSYDSGEVRVVADNPLGTSEHTVKLEIQQREDFRSVLRR-APEQ 233 HSP 2 Score: 67.781 bits (164), Expect = 6.642e-12 Identity = 66/274 (24.09%), Postives = 113/274 (41.24%), Query Frame = 3
Query: 729 PLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTES-----SISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLN 1535
P H E L D ++V EW D L +R+ ++ + GYC+LD Y D+G +C A N G +T S + E+ ++ ++ L E + +++ + + G D P + + P V EG A F+ + K+ +WY NG++++ R + I L I+ T D+G V + G E V L +Q + D +S A AE+ ++ D + D P + Q I +L+
Sbjct: 6 PAHFECRLTPIGDPTMVVEWLHDSKPLAAANRLRMVNEFGYCSLDYEVAYARDSGVITCRASNKFGVDQT-SATLIVKDEKGLVEETQLPEGRKGAYRVDEIERMVHEGGPAGVSADEESEKTKPEIV--LLPEPARVLEGDIARFRCRVTGYPAPKV--NWYLNGQLIRKSKRYRLNYD-GIYYLEIVDTKSYDSGEVRVVADNPLGTSE-HTVKLEIQQRE----DFRSVLRRAPEQRAAEVQQESGKIGFDVVKADRPGEVPQDREIVKLH 268 HSP 3 Score: 66.6254 bits (161), Expect = 1.540e-11 Identity = 52/210 (24.76%), Postives = 94/210 (44.76%), Query Frame = 3
Query: 312 PLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPE--EMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAE 935
P R+ P D ++ + W D K L+A +R + + G+ LD D+G C A + G Q++ A + + E G+ TQLPE + ++ +++ G G+ D+ + KT P+ + LP +++G + + + W+ +G ++ R + YD G L+I D+G+ VA N LG +E
Sbjct: 6 PAHFECRLTPIGDPTMVVEWLHDSKPLAAANRLRMVNEFGYCSLDYEVAYARDSGVITCRASNKFGVDQTS-ATLIVKDEKGLVEETQLPEGRKGAYRVDEIERMVHEGGPAGVSADEESEKTKPEIVL-LPEPARVLEGDIARFRCRVTGYPAPKV--NWYLNGQLIRKSKRYRLNYD-GIYYLEIVDTKSYDSGEVRVVADNPLGTSE 210
BLAST of Titin vs. Ensembl Medaka
Match: ENSORLT00000044036.1 (pep primary_assembly:ASM223467v1:21:968974:997146:1 gene:ENSORLG00000030355.1 transcript:ENSORLT00000044036.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 72.4034 bits (176), Expect = 3.767e-13 Identity = 45/170 (26.47%), Postives = 79/170 (46.47%), Query Frame = 3
Query: 729 PLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTES-----SISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVK 1223
P H E L D ++V EW DG L +R+ ++ + GYC+LD Y D+G +C A N G +T S + E+ ++ ++ L E I +++ L + G Y + + P + + P + V EG A F+ + K+ +WY NG++++
Sbjct: 37 PAHFECRLTPIGDPTMVVEWLHDGKPLAAANRLRMVNEFGYCSLDYEVAYARDSGVITCRATNKFGVDQT-SATLIVKDEKGLVVETQLPEGRKGAHRIDEMERLAHEGGPYGVTAEEQTEKLKPEIV--LLPEPVRVVEGDTARFRCRVTGYPAPKV--NWYLNGQLIR 201 HSP 2 Score: 63.1586 bits (152), Expect = 3.783e-10 Identity = 69/302 (22.85%), Postives = 121/302 (40.07%), Query Frame = 3
Query: 312 PLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPE--EMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNG 1211
P R+ P D ++ + W DGK L+A +R + + G+ LD D+G C A + G Q++ A + + E G+ TQLPE + I+ +++ G +G+ ++ K P+ + LP + +++G + + + W+ +G +I + Y +L I E G+Y C N G++ TC S L L+ P E R D + PP F + I+ E V +G +F+ + GQ L + + G
Sbjct: 37 PAHFECRLTPIGDPTMVVEWLHDGKPLAAANRLRMVNEFGYCSLDYEVAYARDSGVITCRATNKFGVDQTS-ATLIVKDEKGLVVETQLPEGRKGAHRIDEMERLAHEGGPYGVTAEEQTEKLKPEIV-LLPEPVRVVEGDTARFRCRVTGYPAPKV--NWYLNG---------QLIRKKNY-SLVINKTQTEHEGEYFCSVSNPFGQS-------TC---------------SSHLLVQLKEP--ERGREVDLTPHDKPPKFTKAIESSE--VPQGGQCFFRYVV---TGQPLPDIQWLKG 295 HSP 3 Score: 59.6918 bits (143), Expect = 5.126e-9 Identity = 52/211 (24.64%), Postives = 93/211 (44.08%), Query Frame = 3
Query: 1560 VKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEK-IVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLTV 2189
++ P D ++ VEW + + +R+++ + G +L+ A + DSG ATNK G Q+ I + EK +V + L G R+ ++E LA E+ E+ P + P + EG A+F +V+G+P P + W ++ L+ K Y+ L IN+ + + + +NP G + + L V
Sbjct: 42 CRLTPIGDPTMVVEWLHDGKPLAAANRLRMVNEFGYCSLDYEVAYARDSGVITCRATNKFGVDQTSATLI--VKDEKGLVVETQLPEGRKGAHRIDEMER-LAHEGGPYGVTAEEQTEKLKPEIVLLPEPVRVVEGDTARFRCRVTGYPAPKVNWYLNGQLIRK----KNYS------LVINKTQTEHEGEYFCSVSNPFGQSTCSSHLLV 239
BLAST of Titin vs. Ensembl Medaka
Match: ENSORLT00000038377.1 (pep primary_assembly:ASM223467v1:21:27290473:27302032:1 gene:ENSORLG00000027660.1 transcript:ENSORLT00000038377.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 71.633 bits (174), Expect = 2.469e-12 Identity = 119/580 (20.52%), Postives = 199/580 (34.31%), Query Frame = 3
Query: 678 PKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGK-----LEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQ---------------------------------------------------TEKSIILDPQS---KGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLTVI-PMVDFRPDLRHITPE 2237
P F+ +L N +T+I G+ + + + W+ DG ++ G +V + C L I D+G Y C A N G ET + T V+ PPSF+ P P+ + G+ F + + KL T L+GK L+ N L +L S G Y C + ++ G+ ES V L+V+ TE S IL+ S K + ++ + +A K + I + P F++++EP+ ++ GD+V L +V A + V W+K + + + ++ Y +G A L + A D G+Y A NK G S T + + P+F K ++ EG P +F +V+G + W+ D + + D + LEI+R + ATN +G A A LT+ P++D R ++P+
Sbjct: 32 PSFVKKLTN-ITVILGE--EVTLMCKVSGSPPFTISWYHDGTEVQSGPNHEVSFSDNSCTLSIPTLKLSDSGVYKCKAVNKAGSCETTA----------------------------------------TLVVKEPPSFVVP--PQPVEAMPGSKVTFSAIVKGSSPLKLRC-------FRGTKELLSGKDCEFSLKDNQVVLELLNVDRSHAGEYTCQIINDAGK-ESCAVNLSVKGQFLGKHLWLCLLHSLHSVFKCSYTGTPKITVNWYKDGQEIFASYKYNITTTESSCILECLSTDDKESAGKYSCQVSNDAGKDTTEAVVSILEPPYFLEKMEPM-EVTAGDAVCLKCQV--AGTPEIKVSWFKADGKVRSSPTCKLEYSKGVACLKLSKATKADVGEYTCKAENKIGSASS----------------------------------------------TCRLNAKTPPSFPKKITSVQETEGQPIRFECRVAGSSPIEVSWQKDGKPLTHGADFSMLYDDNTAVLEISRGEMRHSGEYTCVATNGVGSASCRAKLTLYPPIID-----RKLSPQ 504 HSP 2 Score: 58.9214 bits (141), Expect = 1.841e-8 Identity = 60/243 (24.69%), Postives = 102/243 (41.98%), Query Frame = 3
Query: 249 LYDSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFK-DGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQ---GQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHP 965
+ + P F + +E + V G+ + L +V A ++++WFK DGK+ S+ + + +G L + + D G+Y C A++ G+A S R+N AKT P F P +T +Q GQP+ E + S + W KDG L G+ ++YD L+I+ +G+Y+CVA N +G A ++ +T +P
Sbjct: 306 ILEPPYFLEKMEP-MEVTAGDAVCLKCQV--AGTPEIKVSWFKADGKVRSSPT-CKLEYSKGVACLKLSKATKADVGEYTCKAENKIGSASST---CRLN----------------------------------------AKTPPSF----PKKITSVQETEGQPIRFECRVA--GSSPIEVSWQKDGKPLTHGADFSMLYDDNTAVLEISRGEMRHSGEYTCVATNGVGSASCRA-KLTLYP 494
BLAST of Titin vs. Ensembl Medaka
Match: ENSORLT00000031689.1 (pep primary_assembly:ASM223467v1:21:27233720:27236532:1 gene:ENSORLG00000023256.1 transcript:ENSORLT00000031689.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 63.929 bits (154), Expect = 1.584e-10 Identity = 69/282 (24.47%), Postives = 124/282 (43.97%), Query Frame = 3
Query: 255 DSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSL--EINWFKDGKLLSA----GSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPM 1082
+PTF Q L+S +V EG +V GS E++WF+DG++LSA G + S F G VL I +G + A + +G A S A +++ E+ P FL +L +S T+ QG + +++ + + V +++++G ++ + ++ + +L IA +PED+G YS A NS G+A T + + EE++ +K T S S +++ + E A + T+++ M
Sbjct: 4 QAPTFTQPLQS-VVALEGSAATFEAQV----SGSPVPEVSWFRDGQVLSAAALPGVQIS--FSDGRAVLRIPAVTAAHSGRFSIRATNGAGQATST-AELQVTAETA---------------------------------------PPNFLQRLQSS-TVRQGSQVRLDVRVT--GIPTPVVKFYREGAEIQSSADFQILQNGDVYSLLIAEAFPEDSGTYSVTATNSSGRA-TSTAELLVQSEETVPSKKTKTTVSSSHMETSQTTKVE-ASFQATTMMEM 233
BLAST of Titin vs. Planmine SMEST
Match: SMESG000041478.1 (SMESG000041478.1) HSP 1 Score: 1696.4 bits (4392), Expect = 0.000e+0 Identity = 820/822 (99.76%), Postives = 821/822 (99.88%), Query Frame = 3
Query: 84 MTTQESMEYNNNSNFNSVPDEGVIPNGTTMAPHALEVVNQVNKRSLASPRRGSDHLYDSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLTVIPMVDFRPDLRHITPENPFKKMAGLKKVECTSELNDAFKKSKPTAERIVKIEMGSETKSKFYKSPDVIETEKLYDQVAAGLRKNIGPVSNNGARSPRNNHSGSPSLTTNEEMPSLNPVNY 2549
MTTQESMEYNNNSNFNSVPDEGVIPNGTTMAPHALEVVNQVNKRSLASPRRGSDHLYDSPTF+QDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLTVIPMVDFRPDLRHITPENPFKKMAGLKKVECTSELNDAFKKSKPTAERIVKIEMGSETKSKFYKSPDVIETEKLYDQVAAGLRKNIGP SNNGARSPRNNHSGSPSLTTNEEMPSLNPVNY
Sbjct: 1 MTTQESMEYNNNSNFNSVPDEGVIPNGTTMAPHALEVVNQVNKRSLASPRRGSDHLYDSPTFRQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLTVIPMVDFRPDLRHITPENPFKKMAGLKKVECTSELNDAFKKSKPTAERIVKIEMGSETKSKFYKSPDVIETEKLYDQVAAGLRKNIGPGSNNGARSPRNNHSGSPSLTTNEEMPSLNPVNY 822
BLAST of Titin vs. Planmine SMEST
Match: SMESG000029197.1 (SMESG000029197.1) HSP 1 Score: 325.479 bits (833), Expect = 4.506e-99 Identity = 186/479 (38.83%), Postives = 278/479 (58.04%), Query Frame = 3
Query: 1083 PPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILD---PQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLE--EDLASGNISTFQNTEDVQEECLPNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLTVIPMVDFRPDLRHITPENPFKKMAGLKKVECTSELNDAFKKSKPTAERIVKIEMGSETKSKFYKSPDVIETEKLYDQVAAGLRKNIGPVSNNGARSPRNNHSGSPS 2504
PPSF++ + +++ +EG PA + P L+ WY +G + SR E + L+ILYT D+G Y CV + G ++ P TLNV ++S++L+ PQ + +I A+EA LN PK + + P F+K + + L G SV L +V P D+SL +EW+KN + NG R I +DRG A L+I+ ISED+GDY+ A NK GQ QS + + E +VT S L +S Y + D++ ED+ N F+ E+ + E LP+FD+KP+N+T EG PA+FLVKVSG+P P ++W +++ LV+P S+ K Y+DGSI+YLE RC G+ + V+A N LG A ATL+VIP DFRP+L+ PENPF+K++ LKKV + EL +AF+K+KP+A ++ E G+ +S + + E LY V + LR RS + +++G+PS
Sbjct: 202 PPSFVKHPERYQVA-NEGDPASLTARVIPAGDPNLTVQWYKDGVPLAAGSRFNTSFERGFAILDILYTFKEDSGEYSCVAKNNFGMDQTSPSTLNVNLDESVVLNSHLPQ-EMTESIAAIEASLNTPKRQDIHIDAPIRPPQFVKPMPGLYNLQGGQSVHLETRVIPITDSSLVIEWFKNGRPLNNGCRFNIVFDRGFAILDIIGVISEDTGDYFCFARNKAGQAQSNVVRLLVATEETVVTKSQLPQESVQYLQSLDIQNTEDVDRSN---FRYEEEEENESLPHFDIKPINLTVEEGNPARFLVKVSGYPRPQIQWFLNECLVIPTSIQKVYSDGSISYLEFQRCKIPGESFIVVSAKNKLGEAVANATLSVIPENDFRPELKRTDPENPFRKLSTLKKVAVSPELQNAFRKTKPSAVQLSNYERGAP------RSKEASDAENLYANVQSRLR-----------RSDKLSYAGAPS 658 HSP 2 Score: 217.238 bits (552), Expect = 1.052e-59 Identity = 124/328 (37.80%), Postives = 187/328 (57.01%), Query Frame = 3
Query: 210 KRSLASPRRGS----DHLYDSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSL-----ENPGEEYARYNDTSVIPMPPSF-IEPIDPREISVSEGAPAYFKVTI 1163
++S S R GS D + D P+F + E V +EG+P L RV PA D +L + W+KDG L+AGSRF+T F+RGF +LDILYT ++D+G+Y CVAK++ G Q++ + + +N + V + LP+EM + I ++ + T + D A P+F+ +P L GQ +H+E + +DSSLV EWFK+G L G R ++++DRG+ LDI ED GDY C ARN G+A++ + EE+++TKS L + S+ L+SL E+ RY + P F I+PI+ ++V EG PA F V +
Sbjct: 182 QQSFRSSRSGSVAHLDEITDPPSFVKHPERYQVANEGDPASLTARVIPAGDPNLTVQWYKDGVPLAAGSRFNTSFERGFAILDILYTFKEDSGEYSCVAKNNFGMDQTSPSTLNVNLDESVVLNSHLPQEMTESIAAIEASLNTPKRQDIHID--APIRPPQFVKPMPGLYNLQGGQSVHLETRVIPITDSSLVIEWFKNGRPLNNGCRFNIVFDRGFAILDIIGVISEDTGDYFCFARNKAGQAQSNVVRLLVATEETVVTKSQLPQESVQYLQSLDIQNTEDVDRSNFRYEEEEENESLPHFDIKPIN---LTVEEGNPARFLVKV 504
BLAST of Titin vs. Planmine SMEST
Match: SMESG000029197.1 (SMESG000029197.1) HSP 1 Score: 319.316 bits (817), Expect = 8.782e-97 Identity = 186/485 (38.35%), Postives = 278/485 (57.32%), Query Frame = 3
Query: 1083 PPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILD---PQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLE--EDLASGNISTFQNTEDVQEECLPNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLTVIPMVDFRPDLRHITP------ENPFKKMAGLKKVECTSELNDAFKKSKPTAERIVKIEMGSETKSKFYKSPDVIETEKLYDQVAAGLRKNIGPVSNNGARSPRNNHSGSPS 2504
PPSF++ + +++ +EG PA + P L+ WY +G + SR E + L+ILYT D+G Y CV + G ++ P TLNV ++S++L+ PQ + +I A+EA LN PK + + P F+K + + L G SV L +V P D+SL +EW+KN + NG R I +DRG A L+I+ ISED+GDY+ A NK GQ QS + + E +VT S L +S Y + D++ ED+ N F+ E+ + E LP+FD+KP+N+T EG PA+FLVKVSG+P P ++W +++ LV+P S+ K Y+DGSI+YLE RC G+ + V+A N LG A ATL+VIP DFRP+L+ P ENPF+K++ LKKV + EL +AF+K+KP+A ++ E G+ +S + + E LY V + LR RS + +++G+PS
Sbjct: 202 PPSFVKHPERYQVA-NEGDPASLTARVIPAGDPNLTVQWYKDGVPLAAGSRFNTSFERGFAILDILYTFKEDSGEYSCVAKNNFGMDQTSPSTLNVNLDESVVLNSHLPQ-EMTESIAAIEASLNTPKRQDIHIDAPIRPPQFVKPMPGLYNLQGGQSVHLETRVIPITDSSLVIEWFKNGRPLNNGCRFNIVFDRGFAILDIIGVISEDTGDYFCFARNKAGQAQSNVVRLLVATEETVVTKSQLPQESVQYLQSLDIQNTEDVDRSN---FRYEEEEENESLPHFDIKPINLTVEEGNPARFLVKVSGYPRPQIQWFLNECLVIPTSIQKVYSDGSISYLEFQRCKIPGESFIVVSAKNKLGEAVANATLSVIPENDFRPELKRTDPAILLRNENPFRKLSTLKKVAVSPELQNAFRKTKPSAVQLSNYERGAP------RSKEASDAENLYANVQSRLR-----------RSDKLSYAGAPS 664 HSP 2 Score: 217.624 bits (553), Expect = 8.374e-60 Identity = 124/328 (37.80%), Postives = 187/328 (57.01%), Query Frame = 3
Query: 210 KRSLASPRRGS----DHLYDSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSL-----ENPGEEYARYNDTSVIPMPPSF-IEPIDPREISVSEGAPAYFKVTI 1163
++S S R GS D + D P+F + E V +EG+P L RV PA D +L + W+KDG L+AGSRF+T F+RGF +LDILYT ++D+G+Y CVAK++ G Q++ + + +N + V + LP+EM + I ++ + T + D A P+F+ +P L GQ +H+E + +DSSLV EWFK+G L G R ++++DRG+ LDI ED GDY C ARN G+A++ + EE+++TKS L + S+ L+SL E+ RY + P F I+PI+ ++V EG PA F V +
Sbjct: 182 QQSFRSSRSGSVAHLDEITDPPSFVKHPERYQVANEGDPASLTARVIPAGDPNLTVQWYKDGVPLAAGSRFNTSFERGFAILDILYTFKEDSGEYSCVAKNNFGMDQTSPSTLNVNLDESVVLNSHLPQEMTESIAAIEASLNTPKRQDIHID--APIRPPQFVKPMPGLYNLQGGQSVHLETRVIPITDSSLVIEWFKNGRPLNNGCRFNIVFDRGFAILDIIGVISEDTGDYFCFARNKAGQAQSNVVRLLVATEETVVTKSQLPQESVQYLQSLDIQNTEDVDRSNFRYEEEEENESLPHFDIKPIN---LTVEEGNPARFLVKV 504
BLAST of Titin vs. Planmine SMEST
Match: SMESG000076529.1 (SMESG000076529.1) HSP 1 Score: 304.679 bits (779), Expect = 2.652e-93 Identity = 173/450 (38.44%), Postives = 257/450 (57.11%), Query Frame = 3
Query: 1086 PSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLTVIPMVDFRPDLRHITPENPFKKMAGLKKVECTSELNDAFKKSKPTAERIVKIEMGSETKSKFYKSPDVIETEKLYDQVAAGLR 2435
P F+ ++ EI+V+E P + P LS +W +G+ + T +R + E L+ILY++ D+G Y+CV S+ G+ +S P T+ V E + D I H + E N P+ K + + P F K LE +N+G +V L V+P ND +L V+W+KN + GSR +DRG L+I+ ED G Y +A N G+ +S I+C EKIV+ S+L + + +LK E ++ F+NTED +++ P+FD ++T EG AKF++ + P ++ W I++ V+ DSV K Y DG+INYLE+ RC+E+G + V ATNPLG + LTV + ++ DL+HI PENP+KKMA LKKV+ T EL A K KPTAE+IVKIE +E K++ K+PDV+E E+LYD+VAA L+
Sbjct: 45 PRFLSNLE-NEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKV--DNNVNFIENQHVQQDE-NEPQYPDKHES--SQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNL--NESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGLQDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTEVGQHNIRVVATNPLGQMASDCILTVNSINNYAQDLKHIAPENPYKKMASLKKVDVTPELTTALHKPKPTAEKIVKIERNAEVKARHTKTPDVLEAEQLYDKVAANLK 486 HSP 2 Score: 263.462 bits (672), Expect = 6.126e-78 Identity = 148/378 (39.15%), Postives = 223/378 (58.99%), Query Frame = 3
Query: 252 YDSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEE----YARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDP----------GNGQKLS---NDWYHNGEI----VKTCSRLTGKLEMNISALNILYTLPSDTGSYICVL 1322
+++P F +LE+E+ V+E +P+RL ++V PA D SL + W KDGK L G+RFST+F+RG++VLDILY+IE+D+G YVCVAKS SG QSN A VR+ PE+ VD E + + + Y DKH + P F L +++T+ QG +H+E ++ +D +L +W K+G S+ LGSR + ++DRGYCALDI + YPED+G Y+CVA+N+ G+AE+ +TC P+E I++KSNL ESSISQLK EN +E R + PSF + + +++++ EG A F + I N + +S N +Y +G I + C+ + G+ + + A N L + SD C+L
Sbjct: 42 FEAPRFLSNLENEITVNEEDPIRLSSQVAPAGDNSLSVEWLKDGKPLLTGTRFSTVFERGYVVLDILYSIEEDSGVYVCVAKSKSGQNQSNPATVRVTPEAKVDNNVNFIENQHVQQDENEPQYP---------DKHESSQSPYFSKDLESNITVNQGGAVHLETIVDPRNDVTLKVDWHKNGQSMSLGSRFNAVFDRGYCALDILYTYPEDDGVYTCVAKNNCGEAESSHSQITCIPDEKIVSKSNLNESSISQLKKFENNIQEEINDAFRNTEDQEDDQAPSFDKGL--QDVTIDEGNAAKFIINIHSHPRASIVWSINNEEVISDSVNKFYGDGTINYLELTRCTEV-GQHNIRVVATNPLGQMASD-----CIL 402
BLAST of Titin vs. Planmine SMEST
Match: SMESG000005752.1 (SMESG000005752.1) HSP 1 Score: 77.411 bits (189), Expect = 5.604e-14 Identity = 162/695 (23.31%), Postives = 249/695 (35.83%), Query Frame = 3
Query: 159 NGTTMAPHALEVVNQVNKR-SLASPRRGSDHLYDS---------PTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEP---VTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKV--EPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISE-DSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLV--VPDSVCKTYNDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLTV 2189
NGT A +L VV + +R S S DS P F+ L+S + V+E +P + RV + EI W+KD + + + L+I +T+E DNG + A + GTA I R+ L P+E+ Q+ P + NS+ + + Q L + + D S T+WF D LK + ++ L I + G Y N++G+ SC +E TKS PP F+ ++ + I V EG +D G + +WY NG +++ + N+ +L I T DTG Y + + G V+S VT+ ++ ++ PQSK I I + PK+ +LN KV P + V WY N+ I + I Y T I++++ E D+G Y + A N G+ Q F V A + + + ++ LE + TA EG P K LV V G P P LEW + VLV +PD + + DG+++ L I + K V A NP G + E L V
Sbjct: 4805 NGTDRADTSLIVVAKPERRLSQQSTSMSRSESVDSKQSSIDGKPPLFKAILQS-VKVEEDKPA--LFRVEFTGNPKPEIKWYKDDQEIVSSPDMKIATSNFITSLEIPHTLEKDNGVFSVRATNFLGTA---ITKGRLT------VLPAKPKEIPQK--------------------------PTIVKPFSNSV-IKENQKLVLVAQTNINKDYS--TKWFLDDKELKPNNVFNISSKLNIHTLSIEKMKSTNAGIYKFKITNNVGE---DSCAAAISLDE---TKST-----------------------------YPPKFVRELE-KSIQVKEGDSVKLLAEVD-GYPTPVV-EWYKNGIVIEKNPDYKLFRDGNVVSLYIHETFTDDTGEYNLIARNPFGSVKSSAHLTVTITPESADNV---PQSKQIPRI------IQEPKSV---------------------KLNLNKKAVFEAKVIGSPVPE----VSWYHNDIFIEESENVSIIYSNNEYTKLIINSVKETDAGTYTVKAVNIVGEAQ--------FSTSLTVEAPTKFPKTEAPKFIRSLEPSM-----------------------------TAKEGEPCKLLVDVMGNPNPKLEWYKNGVLVDNMPD--YQLFRDGNVHTLYIKELFQDDSGKYIVIANNPNGTVQCETNLVV 5347 HSP 2 Score: 73.559 bits (179), Expect = 6.726e-13 Identity = 138/664 (20.78%), Postives = 249/664 (37.50%), Query Frame = 3
Query: 363 INWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTI---PKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRID-VIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPM---------------PPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHI-INGSRMQISYDRGTA-TLNIVSAIS-EDSGDYWLIATNKNGQT-QSEKINISCFEGEKIVTAS-----SLLADSAGYQRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPM-NITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLVVPDSVCKTY-NDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLTVIPMVDFRPDLRHITPENPFKKMAGL 2264
I W+K+G ++ F+ + + L I D+G Y C+ K+ +G + NY T T KTI PKF+ L S +++G + + + +E + +W+KDG + + V+ D +L I P D G Y A+N G+A T E I+ ++ ++E +I + + N + + + +P PP F++ ++P + + EG A+F V GN + + W G ++ + S L I D G + C L ++ GR+ES +K I + EL AP E ++ + F+ Q L+ ++P + + + Y+NE I I+ ++ + T T I+S+ E++G Y ++A N G+T + +I + + +K V + S D+E+D+ V +E F P+ N+ E + KV G+P P L W D + DS K+ N+ ++ L + + A N LG T++V+P ++ ITP K+++ +
Sbjct: 4050 IKWYKNGAEINKSPEFTLSYKENRVSLTIPEVTPKDSGTYKCLVKNVAGDTE---------------------------------NYATVT----------VKTIFMAPKFIFGL-QSQQILEGTNIKLSVKVEGFPTPEI--KWYKDGQVITSSPNFNLVVEDDNIFSLIIPNVRPMDQGKYCVSAKNKAGEAFTMG--------ELIVAENKISEQTIVEQRKKTNVQVDIIVPQNDNRLPQSSKMFSPTKKKEKIQPPEFVKILEP--LKIKEGKKAFFSVEYK-GNPEPVVV-WLREGLQLENGPDYQITTKPGFSQLTIAEVFKDDAGKFTCRLENKAGRIES-----------------ITKLSVGIDKTKTELMAPIFETFPEKYVN---VFVNQ-----------PSKLSCIIKPNSKPEIKI--YQNEKEIKIDNKNYYLNRNEKTGETCFIISSCRWENTGTYKIMAQNPTGKTFATSQIFVKNVDEKKTVISEMESMFERKRSSIRSSVSSDVEKDI-------------VVKESPAKFVQIPLSNVETTEKNIVRLDFKVDGYPYPKLNWLKDGYPLAYDSHIKSVINENGLHSLVFCPLELEDNGQYQCVAQNKLGTDSFSTTISVVP-------VQKITPLRFLKRLSAV 4602 HSP 3 Score: 61.2326 bits (147), Expect = 4.617e-9 Identity = 65/259 (25.10%), Postives = 108/259 (41.70%), Query Frame = 3
Query: 651 EDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDV--IYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHP--EESIITKSNLTESSISQLK-------------------SLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCS--RLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESE 1352
E KTI KF S+L +T + G+ L++E + SDS + +W D + L R+++ I + C L I +D G+Y C+ + +AET + P +E IT ++ +S I +K + + E+ Y + P+FI + P ++V +G + + WYHN EI++ +LT E L I P D G Y CV +++G E+E
Sbjct: 6214 ESSEILKTI-KFKSELEPEITKLVGETLNLECC--YDSDSPVSVKWTLDDVELIESPRVNLALISEVQVCTLTIVNLNEDDTGEYMCIITSDESQAETGTFVTIVQPGEDEQTITVTSSAKSDIEIIKPDLTSIDDSEVSFTFIEKDTKDTKIEKEQEYEIAVELAEAPNFISDLYP--VTVMDGEKLCLSCVVK-ASPMPQKTIWYHNNEIIEPNDELKLTYSTEDGKCLLEIAEVFPEDAGLYKCVARNDYGVAETE 6466 HSP 4 Score: 59.6918 bits (143), Expect = 1.443e-8 Identity = 140/650 (21.54%), Postives = 244/650 (37.54%), Query Frame = 3
Query: 285 SELVVDEGEPLRLMTRVGPADDGSLEIN--WFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSG--TAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRG-------YCALDIAFCYPEDNGDYSCVARNSLG--KAETQSCNVTCHPEESIITKSNLTESSISQLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGY--QRLKDLEEDLASGNISTFQNTEDVQEECLPNFDVKPMNITAGEGGPAKFLVKVSGFPTPTLEWRIDDVLVVPDSVCKTYNDGSINYLEINRCSELGDIKVHVAATNPLGVAKEEATLTV 2189
S + V G P+ + V +GSL + W KDGK + + + L I T D+G Y CVA + +G T + ++ + ES ++ P E M+ I K + + ++A T P F ++ P + G + L + + + WF G L S+ Y++ + +L I C D+ +A+N G +A+T S V PE + +S +S+S+ +S+++ +S+ PP F + + + V E PA F+V GN K WY + + + + + I++L I +TL D G + ++ + L A+ KE I P+ +K + E + L + D S +W+ ++ + + IS TL+I S ++G Y TN G+ SC A SL + Y + +++LE+ +I EG K L +V G+PTP +EW + +++ + K + DG++ L I+ + ++ A NP G K A LTV
Sbjct: 4600 SAVCVQVGTPVTFLCVV----EGSLPVAFIWQKDGKTIDENEKVIIDSEDYSTTLAIENTENSDSGWYQCVASNPAGSITCNARLSFKAVKIESAMN-----PPEWMKAI---PKQKKPANLLSVNIPENAEGTAPCF-TKCPTACKTEPGGTVTFIAELSGYPEPEVT--WFLAGKQL---SKEHPFYEKCLIESHNLHHSLTIRNCQLYDSTRIDVIAQNCNGTDRADT-SLIVVAKPERRLSQQS----TSMSRSESVDS--------KQSSIDGKPPLFKAIL--QSVKVEEDKPALFRVEFT-GNP-KPEIKWYKDDQEIVSSPDMKIATSNFITSLEIPHTLEKDNG----------------------------VFSVRATNFLGTAITKGRLTVLPAKPKE---IPQKPTIVKPFSN-SVIKENQKLVLVAQTNINKDYS--TKWFLDDKELKPNNVFNISSKLNIHTLSIEKMKSTNAGIYKFKITNNVGED-------SC------AAAISLDETKSTYPPKFVRELEK-----------------------------SIQVKEGDSVKLLAEVDGYPTPVVEWYKNGIVIEKNPDYKLFRDGNVVSLYIHETFTDDTGEYNLIARNPFGSVKSSAHLTV 5138 HSP 5 Score: 59.3066 bits (142), Expect = 1.505e-8 Identity = 62/262 (23.66%), Postives = 119/262 (45.42%), Query Frame = 3
Query: 285 SELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKS--HSGTAQSNI------AHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDR--GYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITK----SNLTESSISQLKSL 1028
+++V+ G+ L L + PA D +E WFK+ + + + +D G +L I E+D+G Y+C A S S + SN+ + +R + + +D ++ ++ Q +N++ + A +++ K L LPN +++ + L ++ + ++ + W+ DG L+ S I I ++ G C L + +PED G Y C N +T NV P+ + K S L E ++ + S+
Sbjct: 9451 TDMVLPVGKSLLLECEITPAVDVVVE--WFKNLTPVISNEHIAITYDLGKCILMIAEVEEEDSGHYICKASSRERSISTSSNVTVKNDSSEMRESEDETIDASFEIDDDNKQSTDNVNSEF-------------ALRSVIKEL--LPNEMSIPESGKLLLQCVIVQSPENRVT--WYLDGDLLE-NSGIYEINNKPDGVCQLIVYEAFPEDEGYYCCKIENQFSTIQT-GTNVHIIPDSVAVQKPKIESKLPEKAVEENSSI 9691 HSP 6 Score: 58.9214 bits (141), Expect = 2.074e-8 Identity = 63/259 (24.32%), Postives = 109/259 (42.08%), Query Frame = 3
Query: 240 SDHLYDSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSR--FSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLP------EEMMQEINNLDKNYETGTYHGMEEDKHAAKT--------------IPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIY--DRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQS 944
S + + F+ +LE E+ GE L L D + + W D L R + I + L I+ EDD G+Y+C+ S A++ + P +T E + ++ ++D + + T+ +E+D K P F+S L +T++ G+ L + ++ S T W+ + ++ + + Y + G C L+IA +PED G Y CVARN G AET++
Sbjct: 6215 SSEILKTIKFKSELEPEITKLVGETLNL--ECCYDSDSPVSVKWTLDDVELIESPRVNLALISEVQVCTLTIVNLNEDDTGEYMCIITSDESQAETGTFVTIVQPGEDEQTITVTSSAKSDIEIIKPDLTSIDDSEVSFTF--IEKDTKDTKIEKEQEYEIAVELAEAPNFISDL-YPVTVMDGEKLCLSCVVKA-SPMPQKTIWYHNNEIIEPNDELKLTYSTEDGKCLLEIAEVFPEDAGLYKCVARNDYGVAETET 6467 HSP 7 Score: 58.151 bits (139), Expect = 3.441e-8 Identity = 84/348 (24.14%), Postives = 141/348 (40.52%), Query Frame = 3
Query: 1029 ENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVE--PANDNSLYVEWYKNENHIINGSRMQISY-DRGTATLNIVSAISEDSGDYWLIATNKNGQTQSEKINISCFEGEKIVTASSLLADSAGYQRLK-DLEEDL-----------ASGNISTFQNTEDVQEECLPNF--DVKPMNITAGEGGPAKFLVKVSGFPTPTLEW 2021
+NP EY + T +P+PPSF++P + I +EG A + + + + WY NG + T + N +L I P D+G+Y C++ + G E+ T K+I + P+ I L+++ Q+ EG ++ L+VKVE P + ++WYK+ I + + D +L I + D G Y + A NK G+ + GE IV + + + QR K +++ D+ +S S + E +Q P F ++P+ I EG A F V+ G P P + W
Sbjct: 4002 QNP--EYLPIDLTVSLPIPPSFVKPF--QNIMCNEGCQAVLEGVLQTNSEPIIK--WYKNGAEINKSPEFTLSYKENRVSLTIPEVTPKDSGTYKCLVKNVAGDTENYATV----TVKTIFMAPK-----FIFGLQSQ----------------------------QILEGTNIKLSVKVEGFPTPE----IKWYKDGQVITSSPNFNLVVEDDNIFSLIIPNVRPMDQGKYCVSAKNKAGEAFT--------MGELIVAENKISEQTIVEQRKKTNVQVDIIVPQNDNRLPQSSKMFSPTKKKEKIQP---PEFVKILEPLKI--KEGKKAFFSVEYKGNPEPVVVW 4289 HSP 8 Score: 56.6102 bits (135), Expect = 1.251e-7 Identity = 80/318 (25.16%), Postives = 125/318 (39.31%), Query Frame = 3
Query: 852 CALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTC------HPEESIITKSNLTESSI--SQLKSLENPGEEYARY--------NDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMNISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLT--VKVEPANDNSLYVEWYKNENHIINGSRMQISYDRGTATLNIVSAISEDSGDYWLIATNKNGQTQ 1751
+L + YP G+Y+C+ N G A TQ C VT H EE I S+ + I Q ENP E + Y N+ + PP IE + E E A K+ +P W N +K + ++ K E N L D+G Y CV + +G E++ + +H N+ K +M+ P FIK L+ G+++ L VK +P + +V WYKN N + N +I+ + +L I + S +G Y ++A N G ++
Sbjct: 1321 ASLSMTEIYPSSAGNYTCIINNQFGSATTQ-CQVTVIEEVSDHNEEWI---SDTQDEIIPKHQTGPQENPPEPFDEYIKPIKRRKNEEAPHGSPPKVIEELHNSEEIEGEKAKLQCKIIGNPTPQIT----WLKNNIPLKLNNAISSKSEGNKHMLIFKDLFLEDSGEYKCVANNPYGFAETKAI---------------------MHVTPYYKNSAKEDMEP-------PVFIKPLDKETHEYVGNTLVLDCFVKGKP----TPHVTWYKNGNRLQNKLTCEINKNDNIHSLIIPNINSNTAGYYSVVAVNTVGTSE 1598 HSP 9 Score: 54.6842 bits (130), Expect = 3.975e-7 Identity = 68/280 (24.29%), Postives = 118/280 (42.14%), Query Frame = 3
Query: 774 LVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHPEESIITKS--NLTESSIS----QLKSLENPGEEYARYNDTSVIPMPPSFIEPIDPREISVSEGAPAYFKVTIDPGNGQKLSNDWYHNGEIVKTCSRLTGKLEMN-----ISALNILYTLPSDTGSYICVLFSEHGRVESEPVTLNVQTEKSIILDPQSKGISAIHALEAELNAPKAEMKEDEIITDLPSFIKQLEPIDQLNEGDSVTLTVKVEPAN 1580
+V EWFK+ + I + YD G C L IA ED+G Y C A +S ++ + S NVT + S + +S ++S +S +N E+A S I+ + P E+S+ E + I +++ WY +G++++ +G E+N + L + P D G Y C ++E++ T+ T II D A+ + E P+ ++E+ I D + +PI D+V + +P N
Sbjct: 9473 VVVEWFKNLTPVISNEHIAITYDLGKCILMIAEVEEEDSGHYICKA-SSRERSISTSSNVTVKNDSSEMRESEDETIDASFEIDDDNKQSTDNVNSEFALR----------SVIKELLPNEMSIPESGKLLLQCVIVQSPENRVT--WYLDGDLLEN----SGIYEINNKPDGVCQLIVYEAFPEDEGYYCC-------KIENQFSTIQTGTNVHIIPDS-----VAVQKPKIESKLPEKAVEENSSINDFLLNMDDEQPIT----TDTVEAVYETKPEN 9719 HSP 10 Score: 54.6842 bits (130), Expect = 4.112e-7 Identity = 51/237 (21.52%), Postives = 108/237 (45.57%), Query Frame = 3
Query: 249 LYDSPTFQQDLESELVVDEGEPLRLMTRVGPADDG--SLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIED-DNGDYVCVAKSHSGTAQSN----IAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLHIEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDRGYCALDIAFCYPEDNGDYSCVARNSLGKAET 938
++ +P F L+S+ ++ EG ++L +V +G + EI W+KDG+++++ F+ + + I I+ + D G Y AK+ +G A + +A +I+ ++ V+ + ++ + D + K P+F+ L L + +G+ + E+ + V W ++G+ L+ G + G+ L IA + +D G ++C N G+ E+
Sbjct: 4111 IFMAPKFIFGLQSQQIL-EGTNIKLSVKV----EGFPTPEIKWYKDGQVITSSPNFNLVVEDDNIFSLIIPNVRPMDQGKYCVSAKNKAGEAFTMGELIVAENKISEQTIVEQRKKTNVQVDIIVPQNDNRLPQSSKMFSPTKKKEKIQPPEFVKIL-EPLKIKEGKKAFFSV--EYKGNPEPVVVWLREGLQLENGPDYQITTKPGFSQLTIAEVFKDDAGKFTCRLENKAGRIES 4339 HSP 11 Score: 53.1434 bits (126), Expect = 1.273e-6 Identity = 55/243 (22.63%), Postives = 91/243 (37.45%), Query Frame = 3
Query: 255 DSPTFQQDLESELVVDEGEPLRLMTRVGPADDGSLEINWFKDGKLLSAGSRFSTIFDRGFIVLDILYTIEDDNGDYVCVAKSHSGTAQSNIAHVRINPESGVDYLTQLPEEMMQEINNLDKNYETGTYHGMEEDKHAAKTIPKFLSQLPNSLTLIQGQPLH-----IEIFLEHHSDSSLVTEWFKDGMSLKLGSRIDVIYDR-GYCALDIAFCYPEDNGDYSCVARNSLGKAETQSCNVTCHP 965
++P F + LE + EGEP +L+ V + LE W+K+G L+ + D L I +DD+G Y+ +A + +GT Q V N +Y + T + + PK + QLP + P+H IE+ + W+ +G + + + + G L I D+GDY A N +G A T++ P
Sbjct: 5255 EAPKFIRSLEPSMTAKEGEPCKLLVDVMGNPNPKLE--WYKNGVLVDNMPDYQLFRDGNVHTLYIKELFQDDSGKYIVIANNPNGTVQCETNLVVTN------------------------DYTSSTRSSITVTEDLEMKAPKIVKQLP------EKSPIHSGTKSIELTCQVVGSPKPNLIWYFNGEIINPSEKYKIDDEEDGLVTLTIDNINESDSGDYILRADNDVGVAMTRTMIAYVKP 5465 The following BLAST results are available for this feature:
BLAST of Titin vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of Titin vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 5
BLAST of Titin vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 5
BLAST of Titin vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of Titin vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of Titin vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of Titin vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of Titin vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of Titin vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of Titin vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 5
BLAST of Titin vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of Titin vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 5
BLAST of Titin vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
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