SMED30023272

Overview
NameSMED30023272
Smed IDSMED30023272
Length (bp)944
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30023272 (SMED30023272) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30023272 (SMED30023272) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30023272 (SMED30023272) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30023272 (SMED30023272) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30023272

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 75

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
Smed sexual biotypeSMED30023272h1SMcG0000098 Contig15498newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30023272h1SMcG0000098 Contig15498uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30023272h1SMcG0000083 Contig2295GPL15192PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30023272h1SMcG0000083 Contig31041uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30023272h1SMcG0000083 Contig31041newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30023272h1SMcG0000098 Contig31041uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30023272h1SMcG0000098 Contig31041newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30023272h1SMcG0000098 Contig2295GPL15192PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30023272h1SMcG0000090 Contig31041uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30023272h1SMcG0000090 Contig31041newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
whole organismSMED30023272h1SMcG0000098 Contig51567newmark_estsPMID:27612384
Roberts-Galbraith et al., 2016		whole organism asexual adult colorimetric in situ hybridization evidence
whole organismSMED30023272h1SMcG0000098 Contig51567uc_Smed_v2PMID:27612384
Roberts-Galbraith et al., 2016		whole organism asexual adult colorimetric in situ hybridization evidence
pharynxSMED30023272h1SMcG0000098 dd_Smed_v6_490_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30023272h1SMcG0000098 dd_Smed_v6_490_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
intestinal phagocyteSMED30023272h1SMcG0000098 dd_Smed_v6_490_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30023272h1SMcG0000098 dd_Smed_v6_490_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
pharynxSMED30023272h1SMcG0000090 dd_Smed_v6_490_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30023272h1SMcG0000090 dd_Smed_v6_490_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
intestinal phagocyteSMED30023272h1SMcG0000090 dd_Smed_v6_490_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30023272h1SMcG0000090 dd_Smed_v6_490_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
pharynxSMED30023272h1SMcG0009086 dd_Smed_v6_490_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30023272h1SMcG0009086 dd_Smed_v6_490_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
intestinal phagocyteSMED30023272h1SMcG0009086 dd_Smed_v6_490_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30023272h1SMcG0009086 dd_Smed_v6_490_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
pharynxSMED30023272h1SMcG0008990 dd_Smed_v6_490_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30023272h1SMcG0008990 dd_Smed_v6_490_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
intestinal phagocyteSMED30023272h1SMcG0008990 dd_Smed_v6_490_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30023272h1SMcG0008990 dd_Smed_v6_490_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
pharynxSMED30023272h1SMcG0008966 dd_Smed_v6_490_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30023272h1SMcG0008966 dd_Smed_v6_490_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
intestinal phagocyteSMED30023272h1SMcG0008966 dd_Smed_v6_490_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30023272h1SMcG0008966 dd_Smed_v6_490_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
pharynxSMED30023272h1SMcG0009107 dd_Smed_v6_490_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30023272h1SMcG0009107 dd_Smed_v6_490_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
intestinal phagocyteSMED30023272h1SMcG0009107 dd_Smed_v6_490_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30023272h1SMcG0009107 dd_Smed_v6_490_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
pharynxSMED30023272h1SMcG0009109 dd_Smed_v6_490_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30023272h1SMcG0009109 dd_Smed_v6_490_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
intestinal phagocyteSMED30023272h1SMcG0009109 dd_Smed_v6_490_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30023272h1SMcG0009109 dd_Smed_v6_490_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
pharynxSMED30023272h1SMcG0009106 dd_Smed_v6_490_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30023272h1SMcG0009106 dd_Smed_v6_490_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
intestinal phagocyteSMED30023272h1SMcG0009106 dd_Smed_v6_490_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30023272h1SMcG0009106 dd_Smed_v6_490_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
pharynxSMED30023272h1SMcG0008970 dd_Smed_v6_490_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30023272h1SMcG0008970 dd_Smed_v6_490_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
intestinal phagocyteSMED30023272h1SMcG0008970 dd_Smed_v6_490_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30023272h1SMcG0008970 dd_Smed_v6_490_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
pharynxSMED30023272h1SMcG0009120 dd_Smed_v6_490_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30023272h1SMcG0009120 dd_Smed_v6_490_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
intestinal phagocyteSMED30023272h1SMcG0009120 dd_Smed_v6_490_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30023272h1SMcG0009120 dd_Smed_v6_490_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
pharynxSMED30023272h1SMcG0009147 dd_Smed_v6_490_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30023272h1SMcG0009147 dd_Smed_v6_490_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
intestinal phagocyteSMED30023272h1SMcG0009147 dd_Smed_v6_490_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30023272h1SMcG0009147 dd_Smed_v6_490_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
pharynxSMED30023272h1SMcG0009192 dd_Smed_v6_490_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30023272h1SMcG0009192 dd_Smed_v6_490_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
intestinal phagocyteSMED30023272h1SMcG0009192 dd_Smed_v6_490_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30023272h1SMcG0009192 dd_Smed_v6_490_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
pharynxSMED30023272h1SMcG0009184 dd_Smed_v6_490_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30023272h1SMcG0009184 dd_Smed_v6_490_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
intestinal phagocyteSMED30023272h1SMcG0009184 dd_Smed_v6_490_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30023272h1SMcG0009184 dd_Smed_v6_490_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
ovarySMED30023272h1SMcG0000036 Contig1624GPL15192PMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite colorimetric in situ hybridization evidence
ovarySMED30023272h1SMcG0000090 Contig1624GPL15192PMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite colorimetric in situ hybridization evidence
ovarySMED30023272h1SMcG0000132 Contig1624GPL15192PMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite colorimetric in situ hybridization evidence
ovarySMED30023272h1SMcG0000196 Contig1624GPL15192PMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite colorimetric in situ hybridization evidence
ovarySMED30023272h1SMcG0000098 Contig1624GPL15192PMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite colorimetric in situ hybridization evidence
ovarySMED30023272h1SMcG0000133 Contig1624GPL15192PMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite colorimetric in situ hybridization evidence
ovarySMED30023272h1SMcG0000257 Contig1624GPL15192PMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite colorimetric in situ hybridization evidence
ovarySMED30023272h1SMcG0000107 Contig1624GPL15192PMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite colorimetric in situ hybridization evidence
ovarySMED30023272h1SMcG0000026 Contig1624GPL15192PMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite colorimetric in situ hybridization evidence
ovarySMED30023272h1SMcG0000263 Contig1624GPL15192PMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite colorimetric in situ hybridization evidence
ovarySMED30023272h1SMcG0021921 Contig1624GPL15192PMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite colorimetric in situ hybridization evidence
Note: Hover over icons to view figure legend
Alignments
SMED30023272 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.002431:13492..18229 -4232
v31.000007:330599..331516 -3198
Homology
[Close]
BLAST of SMED30023272 vs. Planmine SMEST
Match: SMESG000029692.1 (SMESG000029692.1)

HSP 1 Score: 196.823 bits (499), Expect = 2.154e-59
Identity = 104/122 (85.25%), Postives = 109/122 (89.34%), Query Frame = 2 
Query:  518 IEKRMESMTSEIVQLTERMRLMEEKDGEKQGEIERLKGENDKLIQKLNAIKLRMCHASLKGNDIIQLCRIDNSLLSDEMTKLNAEISQSKRQIESIEKQIETSQTKSERQIDAFKQNFEQQQ 883
             E +MESMTS+  QLT+RM LMEEKD E +GEIERLKGENDKL QKLNA+KLRMCHASLK ND IQLCRIDNSLLSDEM KLN EISQSKRQIESIEKQIETSQTKSERQIDAFKQN EQQQ
Sbjct:  163 FENQMESMTSKNDQLTQRMCLMEEKDRENRGEIERLKGENDKLNQKLNAMKLRMCHASLKANDNIQLCRIDNSLLSDEMAKLNTEISQSKRQIESIEKQIETSQTKSERQIDAFKQNCEQQQ 284
[Close]
BLAST of SMED30023272 vs. Planmine SMEST
Match: SMESG000000502.1 (SMESG000000502.1)

HSP 1 Score: 196.052 bits (497), Expect = 4.792e-59
Identity = 105/129 (81.40%), Postives = 112/129 (86.82%), Query Frame = 2 
Query:  518 IEKRMESMTSEIVQLTERMRLMEEKDGEKQGEIERLKGENDKLIQKLNAIKLRMCHASLKGNDIIQLCRIDNSLLSDEMTKLNAEISQSKRQIESIEKQIETSQTKSERQIDAFKQNFEQ---QQNIKV 895
             E +MESMTS+  QLT+RM LMEEKD E +GEIERL GENDKL Q+LNA+KLRMCHASLK ND IQLCRIDNSLLSDEM KLN EISQSKRQIESIEKQIETSQTKSERQIDAFKQN EQ   QQNIK+
Sbjct:  163 FENQMESMTSKNDQLTQRMCLMEEKDRENRGEIERLNGENDKLNQQLNAMKLRMCHASLKANDNIQLCRIDNSLLSDEMAKLNTEISQSKRQIESIEKQIETSQTKSERQIDAFKQNCEQQKLQQNIKI 291
[Close]
BLAST of SMED30023272 vs. Planmine SMEST
Match: SMESG000000502.1 (SMESG000000502.1)

HSP 1 Score: 195.667 bits (496), Expect = 5.616e-59
Identity = 105/129 (81.40%), Postives = 112/129 (86.82%), Query Frame = 2 
Query:  518 IEKRMESMTSEIVQLTERMRLMEEKDGEKQGEIERLKGENDKLIQKLNAIKLRMCHASLKGNDIIQLCRIDNSLLSDEMTKLNAEISQSKRQIESIEKQIETSQTKSERQIDAFKQNFEQ---QQNIKV 895
             E +MESMTS+  QLT+RM LMEEKD E +GEIERL GENDKL Q+LNA+KLRMCHASLK ND IQLCRIDNSLLSDEM KLN EISQSKRQIESIEKQIETSQTKSERQIDAFKQN EQ   QQNIK+
Sbjct:  156 FENQMESMTSKNDQLTQRMCLMEEKDRENRGEIERLNGENDKLNQQLNAMKLRMCHASLKANDNIQLCRIDNSLLSDEMAKLNTEISQSKRQIESIEKQIETSQTKSERQIDAFKQNCEQQKLQQNIKI 284
[Close]
BLAST of SMED30023272 vs. Planmine SMEST
Match: SMESG000029692.1 (SMESG000029692.1)

HSP 1 Score: 198.364 bits (503), Expect = 5.687e-57
Identity = 104/122 (85.25%), Postives = 109/122 (89.34%), Query Frame = 2 
Query:  518 IEKRMESMTSEIVQLTERMRLMEEKDGEKQGEIERLKGENDKLIQKLNAIKLRMCHASLKGNDIIQLCRIDNSLLSDEMTKLNAEISQSKRQIESIEKQIETSQTKSERQIDAFKQNFEQQQ 883
             E +MESMTS+  QLT+RM LMEEKD E +GEIERLKGENDKL QKLNA+KLRMCHASLK ND IQLCRIDNSLLSDEM KLN EISQSKRQIESIEKQIETSQTKSERQIDAFKQN EQQQ
Sbjct:  163 FENQMESMTSKNDQLTQRMCLMEEKDRENRGEIERLKGENDKLNQKLNAMKLRMCHASLKANDNIQLCRIDNSLLSDEMAKLNTEISQSKRQIESIEKQIETSQTKSERQIDAFKQNCEQQQ 284
[Close]
BLAST of SMED30023272 vs. Planmine SMEST
Match: SMESG000000514.1 (SMESG000000514.1)

HSP 1 Score: 186.808 bits (473), Expect = 1.787e-55
Identity = 99/119 (83.19%), Postives = 106/119 (89.08%), Query Frame = 2 
Query:  518 IEKRMESMTSEIVQLTERMRLMEEKDGEKQGEIERLKGENDKLIQKLNAIKLRMCHASLKGNDIIQLCRIDNSLLSDEMTKLNAEISQSKRQIESIEKQIETSQTKSERQIDAFKQNFE 874
             E +MESMTS+  QLT+RM  MEEKD E +GEIERLKGENDKL Q+LNA+KLRMCHASLK ND IQLCRIDNSLLSDEM KLNAEISQSK+QIESIEKQIETSQTKSERQIDAFKQN E
Sbjct:  163 FENQMESMTSKNDQLTQRMCRMEEKDRENRGEIERLKGENDKLNQQLNAMKLRMCHASLKANDNIQLCRIDNSLLSDEMAKLNAEISQSKQQIESIEKQIETSQTKSERQIDAFKQNCE 281
The following BLAST results are available for this feature:
BLAST of SMED30023272 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023272 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023272 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023272 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023272 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023272 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023272 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023272 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023272 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023272 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023272 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023272 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023272 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023272 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000029692.12.154e-5985.25SMESG000029692.1[more]
SMESG000000502.14.792e-5981.40SMESG000000502.1[more]
SMESG000000502.15.616e-5981.40SMESG000000502.1[more]
SMESG000029692.15.687e-5785.25SMESG000029692.1[more]
SMESG000000514.11.787e-5583.19SMESG000000514.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30023272 ID=SMED30023272|Name=SMED30023272|organism=Schmidtea mediterranea sexual|type=transcript|length=944bp
GCACTTAGTCTTAATGATTTTTATCAGATTGTCAGTAGGCGGTTTGAGTA
TATTTTATTTGAGGCTTTTTACAAGAGCTTATTCAAAATTATTCAAAAAA
AGTTGCTCTAGAAAATTTAAATTAGAAAAAAATTTTTCGAAAAATAAATA
CGCACTCAAAAATCCATTATCTTAATTACGTAATGATGTAATCAAGATTA
ATTGCCTAATTGTGTAATTTAATCTGATTCACATCCCCTCAGGGGGCTAT
TATAGATTTTTTTTGTGTTTGGTTCCAGAGGAGACTTAACTACCTACTCA
CACACACATTTATTATTTGTTAGATGTCTATTTAGCTCCAATTTTATTTA
TAATTTAGATTATTCAAACTTGAATATATTTGCAATGCAAATTTAGATCA
GAATGAACTTTTGTTATTTTTATGCAATTTATGTTTATAAATCTTATAAT
AAATATTTGAAATTAAACATTTTTTTATCTATATCAAAATTTAAATTGTG
TTAAATTGAAATTTTAAATAGAAAAAAGGATGGAATCAATGACGAGTGAA
ATTGTTCAATTGACGGAGAGAATGCGCCTGATGGAAGAGAAAGATGGGGA
AAAACAGGGAGAGATTGAGAGATTGAAGGGAGAAAACGATAAACTCATTC
AGAAATTAAATGCAATAAAGCTGAGAATGTGCCACGCGTCACTGAAAGGG
AATGACATCATCCAACTTTGCCGCATTGATAATTCTTTGTTGAGTGATGA
AATGACCAAATTGAATGCAGAAATCAGCCAATCCAAACGGCAAATTGAAA
GCATTGAGAAACAGATCGAGACGAGCCAAACAAAATCCGAACGGCAAATT
GATGCATTTAAACAAAACTTTGAACAGCAGCAGAATATAAAAGTGATTTA
TAGAAATGATTGAATTTGTGATTCAGAATGAAATGGAGAAATGC
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protein sequence of SMED30023272-orf-1

>SMED30023272-orf-1 ID=SMED30023272-orf-1|Name=SMED30023272-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=128bp
MESMTSEIVQLTERMRLMEEKDGEKQGEIERLKGENDKLIQKLNAIKLRM
CHASLKGNDIIQLCRIDNSLLSDEMTKLNAEISQSKRQIESIEKQIETSQ
TKSERQIDAFKQNFEQQQNIKVIYRND*
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