tRNA (Uracil-5-)-methyltransferase-like protein

Overview
NametRNA (Uracil-5-)-methyltransferase-like protein
Smed IDSMED30023251
Length (bp)1465
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of tRNA (Uracil-5-)-methyltransferase-like protein (SMED30023251) t-SNE clustered cells

Violin plots show distribution of expression levels for tRNA (Uracil-5-)-methyltransferase-like protein (SMED30023251) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of tRNA (Uracil-5-)-methyltransferase-like protein (SMED30023251) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for tRNA (Uracil-5-)-methyltransferase-like protein (SMED30023251) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30023251

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 2

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30023251SMESG000077010.1 SmedASXL_013506SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
X2 cellSMED30023251SMESG000077010.1 SmedASXL_013506SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of tRNA (Uracil-5-)-methyltransferase-like protein vs. Ensembl Human
Match: TRMT2B (tRNA methyltransferase 2 homolog B [Source:HGNC Symbol;Acc:HGNC:25748])

HSP 1 Score: 143.28 bits (360), Expect = 8.570e-37
Identity = 124/438 (28.31%), Postives = 220/438 (50.23%), Query Frame = 3 
Query:  132 KKILNTTAPLLSLPYDAQLITKQYKIFSAMQNISSLLRKSNTLSPEILEYCSKNKGLLCKIEDIYKSPVIEGYRNKMGAIVGFDEMGNL-TVGHAMTFSNFDKTRKKLVTFSHFCDKLDKKHHILIAKLFEKFIKEDSQFIPNQKKKISLYKGITIRSNRKNETMINIHFRARFTGIDRILGISKELSDQFLTSLQNESINVRSLYCQQMESHFEHTHAQLSCKLIYGDKYLMENVCGKTFYISPLSFFQVNVEVAEMIYQKAKQLVEDYKSKSGKKIXXXXXXXXXXXXXIILSDIVEQVIGVDIVPSAIEXXXXXXXXXXXXXXEHFCKPVEEFLPEFLNSDIAKEYDNVVAVLNPSRDGLSTEFCETLRSLESLKMIVYISCHVEGQTTLNICTLCTVKGDSNPEV--PFSLSKIIPLDPFPHTTHVEVIFQMTR 1436
            +++ +   PL  L Y+ QL  K       +Q + S ++  N +S  +     K++ L C +  I  SPVI GYRNK    V     GN  TVG       +       V  +H  +  +K  H  +A+ +E F+++          +   ++ +T+R+N +  TM  I F  +    +  L + KE+  +F       +  + SLY Q+  +    +H Q   +L++G+ Y+ E +      ISP +FFQ+N   AEM+Y+   +L     +      +LLD+CCGTG +G+ L+    +V+G++++  A+E+A++ A  N + N E      E+ LP  L S   ++  ++VAV+NP+R GL  +  + +R+  ++  +V++SC + G++T N+  LC     +   +  PF L + +P+D FPHT H E++   TR
Sbjct:   82 ERLADVVTPLWRLSYEEQLKVKFAAQKKILQRLESYIQMLNGVS--VTTAVPKSERLSCLLHPIIPSPVINGYRNKSTFSVNRGPDGNPKTVG--FYLGTWRDGNVVCVQSNHLKNIPEK--HSQVAQYYEVFLRQSPLEPCLVFHEGGYWRELTVRTNSQGHTMAIITFHPQKLSQEE-LHVQKEIVKEFFIRGPGAACGLTSLYFQE-STMTRCSHQQSPYQLLFGEPYIFEELLSLKIRISPDAFFQINTAGAEMLYRTVGEL-----TGVNSDTILLDICCGTGVIGLSLAQHTSRVLGIELLEQAVEDARWTAAFNGITNSEFHTGQAEKILPGLLKSK--EDGQSIVAVVNPARAGLHYKVIQAIRNFRAIHTLVFVSCKLHGESTRNVIELCCPPDPAKKLLGEPFVLQQAVPVDLFPHTPHCELVLLFTR 504
[Close]
BLAST of tRNA (Uracil-5-)-methyltransferase-like protein vs. Ensembl Human
Match: TRMT2B (tRNA methyltransferase 2 homolog B [Source:HGNC Symbol;Acc:HGNC:25748])

HSP 1 Score: 143.28 bits (360), Expect = 8.570e-37
Identity = 124/438 (28.31%), Postives = 220/438 (50.23%), Query Frame = 3 
Query:  132 KKILNTTAPLLSLPYDAQLITKQYKIFSAMQNISSLLRKSNTLSPEILEYCSKNKGLLCKIEDIYKSPVIEGYRNKMGAIVGFDEMGNL-TVGHAMTFSNFDKTRKKLVTFSHFCDKLDKKHHILIAKLFEKFIKEDSQFIPNQKKKISLYKGITIRSNRKNETMINIHFRARFTGIDRILGISKELSDQFLTSLQNESINVRSLYCQQMESHFEHTHAQLSCKLIYGDKYLMENVCGKTFYISPLSFFQVNVEVAEMIYQKAKQLVEDYKSKSGKKIXXXXXXXXXXXXXIILSDIVEQVIGVDIVPSAIEXXXXXXXXXXXXXXEHFCKPVEEFLPEFLNSDIAKEYDNVVAVLNPSRDGLSTEFCETLRSLESLKMIVYISCHVEGQTTLNICTLCTVKGDSNPEV--PFSLSKIIPLDPFPHTTHVEVIFQMTR 1436
            +++ +   PL  L Y+ QL  K       +Q + S ++  N +S  +     K++ L C +  I  SPVI GYRNK    V     GN  TVG       +       V  +H  +  +K  H  +A+ +E F+++          +   ++ +T+R+N +  TM  I F  +    +  L + KE+  +F       +  + SLY Q+  +    +H Q   +L++G+ Y+ E +      ISP +FFQ+N   AEM+Y+   +L     +      +LLD+CCGTG +G+ L+    +V+G++++  A+E+A++ A  N + N E      E+ LP  L S   ++  ++VAV+NP+R GL  +  + +R+  ++  +V++SC + G++T N+  LC     +   +  PF L + +P+D FPHT H E++   TR
Sbjct:   82 ERLADVVTPLWRLSYEEQLKVKFAAQKKILQRLESYIQMLNGVS--VTTAVPKSERLSCLLHPIIPSPVINGYRNKSTFSVNRGPDGNPKTVG--FYLGTWRDGNVVCVQSNHLKNIPEK--HSQVAQYYEVFLRQSPLEPCLVFHEGGYWRELTVRTNSQGHTMAIITFHPQKLSQEE-LHVQKEIVKEFFIRGPGAACGLTSLYFQE-STMTRCSHQQSPYQLLFGEPYIFEELLSLKIRISPDAFFQINTAGAEMLYRTVGEL-----TGVNSDTILLDICCGTGVIGLSLAQHTSRVLGIELLEQAVEDARWTAAFNGITNSEFHTGQAEKILPGLLKSK--EDGQSIVAVVNPARAGLHYKVIQAIRNFRAIHTLVFVSCKLHGESTRNVIELCCPPDPAKKLLGEPFVLQQAVPVDLFPHTPHCELVLLFTR 504
[Close]
BLAST of tRNA (Uracil-5-)-methyltransferase-like protein vs. Ensembl Human
Match: TRMT2B (tRNA methyltransferase 2 homolog B [Source:HGNC Symbol;Acc:HGNC:25748])

HSP 1 Score: 143.28 bits (360), Expect = 8.570e-37
Identity = 124/438 (28.31%), Postives = 220/438 (50.23%), Query Frame = 3 
Query:  132 KKILNTTAPLLSLPYDAQLITKQYKIFSAMQNISSLLRKSNTLSPEILEYCSKNKGLLCKIEDIYKSPVIEGYRNKMGAIVGFDEMGNL-TVGHAMTFSNFDKTRKKLVTFSHFCDKLDKKHHILIAKLFEKFIKEDSQFIPNQKKKISLYKGITIRSNRKNETMINIHFRARFTGIDRILGISKELSDQFLTSLQNESINVRSLYCQQMESHFEHTHAQLSCKLIYGDKYLMENVCGKTFYISPLSFFQVNVEVAEMIYQKAKQLVEDYKSKSGKKIXXXXXXXXXXXXXIILSDIVEQVIGVDIVPSAIEXXXXXXXXXXXXXXEHFCKPVEEFLPEFLNSDIAKEYDNVVAVLNPSRDGLSTEFCETLRSLESLKMIVYISCHVEGQTTLNICTLCTVKGDSNPEV--PFSLSKIIPLDPFPHTTHVEVIFQMTR 1436
            +++ +   PL  L Y+ QL  K       +Q + S ++  N +S  +     K++ L C +  I  SPVI GYRNK    V     GN  TVG       +       V  +H  +  +K  H  +A+ +E F+++          +   ++ +T+R+N +  TM  I F  +    +  L + KE+  +F       +  + SLY Q+  +    +H Q   +L++G+ Y+ E +      ISP +FFQ+N   AEM+Y+   +L     +      +LLD+CCGTG +G+ L+    +V+G++++  A+E+A++ A  N + N E      E+ LP  L S   ++  ++VAV+NP+R GL  +  + +R+  ++  +V++SC + G++T N+  LC     +   +  PF L + +P+D FPHT H E++   TR
Sbjct:   82 ERLADVVTPLWRLSYEEQLKVKFAAQKKILQRLESYIQMLNGVS--VTTAVPKSERLSCLLHPIIPSPVINGYRNKSTFSVNRGPDGNPKTVG--FYLGTWRDGNVVCVQSNHLKNIPEK--HSQVAQYYEVFLRQSPLEPCLVFHEGGYWRELTVRTNSQGHTMAIITFHPQKLSQEE-LHVQKEIVKEFFIRGPGAACGLTSLYFQE-STMTRCSHQQSPYQLLFGEPYIFEELLSLKIRISPDAFFQINTAGAEMLYRTVGEL-----TGVNSDTILLDICCGTGVIGLSLAQHTSRVLGIELLEQAVEDARWTAAFNGITNSEFHTGQAEKILPGLLKSK--EDGQSIVAVVNPARAGLHYKVIQAIRNFRAIHTLVFVSCKLHGESTRNVIELCCPPDPAKKLLGEPFVLQQAVPVDLFPHTPHCELVLLFTR 504
[Close]
BLAST of tRNA (Uracil-5-)-methyltransferase-like protein vs. Ensembl Human
Match: TRMT2A (tRNA methyltransferase 2 homolog A [Source:HGNC Symbol;Acc:HGNC:24974])

HSP 1 Score: 137.887 bits (346), Expect = 2.421e-34
Identity = 120/445 (26.97%), Postives = 218/445 (48.99%), Query Frame = 3 
Query:  135 KILNTTAPLLSLPYDAQLITKQYKIFSAMQNISSLLRKSN-TLSPEILEYCSKNKGLLCKIEDIYKSPVIEGYRNKMGAIVGFD----------EMGNLTVGHAMTFSNFDKTRKKLVTFSHFCDKLDKKHHILIAKLFEKFIKEDSQFIPNQKKKISLYKGITIRSNRKNETMINIHFRARFTGIDRILGISKELSDQFLTSLQNESINVRSLYCQQMESHFEHTHAQLSCKLIYGDKYLMENVCGKTFYISPLSFFQVNVEVAEMIYQKAKQLVEDYKSKSGKKIXXXXXXXXXXXXXIILSDIVEQVIGVDIVPSAIEXXXXXXXXXXXXXXEHFCKPVEEFLPEFLNSDIAKEYDNVVAVLNPSRDGLSTEFCETLRSLESLKMIVYISCHVEGQTTLNICTLCTVKGDSNPEVPFSLSKIIPLDPFPHTTHVEVIFQMTR 1436
            ++ +   PL ++PY  QL  KQ +    +Q ++  +  +N  L P +LE   K+    C +E +  SP    YRNK   +VG             +G    G     + FD          H  +   +     + K F++FI+       + +     +K +T+R++R+++ M   +F  +    + +  +   L+  F T+    +  V  LY  +       +   L  + + GD+ + E++ G TF ISP +FFQVN   AE++Y     +++D+       + +LD+CCGTGT+G+ L+  V++VIGV++ P A+E+A+ NA+ N ++NVE  C   E+ +P  + S +A ++  +VA+L+P R GL ++    +R  ++L+ ++Y+SC+       N   LC    +    +PF   K + +D FP T H E++    R
Sbjct:  167 RVADVVTPLWTVPYAEQLERKQLECEQVLQKLAKEIGSTNRALLPWLLEQRHKHNKACCPLEGVRPSPQQTEYRNKCEFLVGVGVDGEDNTVGCRLGKYKGGTCAVAAPFDTV--------HIPEATKQ-----VVKAFQEFIRSTPYSAYDPETYTGHWKQLTVRTSRRHQAMAIAYFHPQKLSPEELAELKTSLAQHF-TAGPGRASGVTCLYFVEEGQRKTPSQEGLPLEHVAGDRCIHEDLLGLTFRISPHAFFQVNTPAAEVLY----TVIQDWAQLDAGSM-VLDVCCGTGTIGLALARKVKRVIGVELCPEAVEDARVNAQDNELSNVEFHCGRAEDLVPTLV-SRLASQH--LVAILDPPRAGLHSKVILAIRRAKNLRRLLYVSCNPRAAMG-NFVDLCRAPSNRVKGIPFRPVKAVAVDLFPQTPHCEMLILFER 588
[Close]
BLAST of tRNA (Uracil-5-)-methyltransferase-like protein vs. Ensembl Human
Match: TRMT2A (tRNA methyltransferase 2 homolog A [Source:HGNC Symbol;Acc:HGNC:24974])

HSP 1 Score: 137.887 bits (346), Expect = 2.421e-34
Identity = 120/445 (26.97%), Postives = 218/445 (48.99%), Query Frame = 3 
Query:  135 KILNTTAPLLSLPYDAQLITKQYKIFSAMQNISSLLRKSN-TLSPEILEYCSKNKGLLCKIEDIYKSPVIEGYRNKMGAIVGFD----------EMGNLTVGHAMTFSNFDKTRKKLVTFSHFCDKLDKKHHILIAKLFEKFIKEDSQFIPNQKKKISLYKGITIRSNRKNETMINIHFRARFTGIDRILGISKELSDQFLTSLQNESINVRSLYCQQMESHFEHTHAQLSCKLIYGDKYLMENVCGKTFYISPLSFFQVNVEVAEMIYQKAKQLVEDYKSKSGKKIXXXXXXXXXXXXXIILSDIVEQVIGVDIVPSAIEXXXXXXXXXXXXXXEHFCKPVEEFLPEFLNSDIAKEYDNVVAVLNPSRDGLSTEFCETLRSLESLKMIVYISCHVEGQTTLNICTLCTVKGDSNPEVPFSLSKIIPLDPFPHTTHVEVIFQMTR 1436
            ++ +   PL ++PY  QL  KQ +    +Q ++  +  +N  L P +LE   K+    C +E +  SP    YRNK   +VG             +G    G     + FD          H  +   +     + K F++FI+       + +     +K +T+R++R+++ M   +F  +    + +  +   L+  F T+    +  V  LY  +       +   L  + + GD+ + E++ G TF ISP +FFQVN   AE++Y     +++D+       + +LD+CCGTGT+G+ L+  V++VIGV++ P A+E+A+ NA+ N ++NVE  C   E+ +P  + S +A ++  +VA+L+P R GL ++    +R  ++L+ ++Y+SC+       N   LC    +    +PF   K + +D FP T H E++    R
Sbjct:  167 RVADVVTPLWTVPYAEQLERKQLECEQVLQKLAKEIGSTNRALLPWLLEQRHKHNKACCPLEGVRPSPQQTEYRNKCEFLVGVGVDGEDNTVGCRLGKYKGGTCAVAAPFDTV--------HIPEATKQ-----VVKAFQEFIRSTPYSAYDPETYTGHWKQLTVRTSRRHQAMAIAYFHPQKLSPEELAELKTSLAQHF-TAGPGRASGVTCLYFVEEGQRKTPSQEGLPLEHVAGDRCIHEDLLGLTFRISPHAFFQVNTPAAEVLY----TVIQDWAQLDAGSM-VLDVCCGTGTIGLALARKVKRVIGVELCPEAVEDARVNAQDNELSNVEFHCGRAEDLVPTLV-SRLASQH--LVAILDPPRAGLHSKVILAIRRAKNLRRLLYVSCNPRAAMG-NFVDLCRAPSNRVKGIPFRPVKAVAVDLFPQTPHCEMLILFER 588
[Close]
BLAST of tRNA (Uracil-5-)-methyltransferase-like protein vs. Ensembl Celegans
Match: trm-2A (TRna (tRNA) Methyltransferase homolog [Source:UniProtKB/TrEMBL;Acc:Q9N5L0])

HSP 1 Score: 62.3882 bits (150), Expect = 3.659e-10
Identity = 64/264 (24.24%), Postives = 114/264 (43.18%), Query Frame = 3 
Query:  126 EMKKILNTTAPLLSLPYDAQLITKQYKIFSAMQNISSLLRKSNTLSPEILEYCSKNKGLLCKI-EDIYKSPVIEGYRNKMGAIVGFDEMGNLTVGHAMTFSNFDKTRKKLVTFSHFCDKLDKKHHILIAKLFEKFIKEDSQFIPNQKKKISLYKGITIRSNRKNETMINIHFRARFTGIDRILGISKELSDQFLT--SLQNESINVRSLYCQQMESHFEHTHAQLSCKLIYGDKYLMENVCGKTFYISPLSFFQVNVEVAEMIY 908
            + K    +  PL  + Y  QL  KQ      ++ +   L ++       + +  K      ++  +I  +P   GYRNK    +G D  G + VG       F K  +  V      D +  +   ++  + E F+KE +    N+  +   ++ +TIR    +  +I   F     G++R+  I K++  +FL   + + +   V S+Y QQM     H     + KLI G  Y+ E++    F +SP +FFQ N + A ++Y
Sbjct:  117 QQKTARESVTPLAEMEYGKQLELKQQNSVGVVEKLYRQLIQNK------VAHLGKQTQFRDRVLREILPAPKTAGYRNKCEFTIGHDLNGEICVG--FVGGRFSKN-QHFVIPPVDVDIVSTQMMAIVGDVHE-FVKESALEPFNEFDRKGFWRMLTIREFGGDVMLIFTVFPLE-DGVERMEEIQKKIETRFLDFETFKEKKYRVCSIYWQQMV----HISDTPNFKLIGGTPYIYESLLDCRFRVSPPAFFQTNSQAAAVLY 365

HSP 2 Score: 57.3806 bits (137), Expect = 1.462e-8
Identity = 27/85 (31.76%), Postives = 46/85 (54.12%), Query Frame = 3 
Query: 1182 VVAVLNPSRDGLSTEFCETLRSLESLKMIVYISCHVEGQTTLNICTLCTVKGDSNPEVPFSLSKIIPLDPFPHTTHVEVIFQMTR 1436
            VV VL+P R G+  +     R +E+++ ++++SC      + NI  LC           FS++++ P+D FP T H+E I Q+ R
Sbjct:  520 VVGVLDPPRAGMHEKVILACREMETMRRLIFVSCEPNAAIS-NIVNLCRPSSRKYTGDAFSVTRVQPVDMFPQTNHMEWIIQLDR 603
[Close]
BLAST of tRNA (Uracil-5-)-methyltransferase-like protein vs. Ensembl Celegans
Match: trm-2A (TRna (tRNA) Methyltransferase homolog [Source:UniProtKB/TrEMBL;Acc:Q9N5L0])

HSP 1 Score: 62.3882 bits (150), Expect = 3.659e-10
Identity = 64/264 (24.24%), Postives = 114/264 (43.18%), Query Frame = 3 
Query:  126 EMKKILNTTAPLLSLPYDAQLITKQYKIFSAMQNISSLLRKSNTLSPEILEYCSKNKGLLCKI-EDIYKSPVIEGYRNKMGAIVGFDEMGNLTVGHAMTFSNFDKTRKKLVTFSHFCDKLDKKHHILIAKLFEKFIKEDSQFIPNQKKKISLYKGITIRSNRKNETMINIHFRARFTGIDRILGISKELSDQFLT--SLQNESINVRSLYCQQMESHFEHTHAQLSCKLIYGDKYLMENVCGKTFYISPLSFFQVNVEVAEMIY 908
            + K    +  PL  + Y  QL  KQ      ++ +   L ++       + +  K      ++  +I  +P   GYRNK    +G D  G + VG       F K  +  V      D +  +   ++  + E F+KE +    N+  +   ++ +TIR    +  +I   F     G++R+  I K++  +FL   + + +   V S+Y QQM     H     + KLI G  Y+ E++    F +SP +FFQ N + A ++Y
Sbjct:  117 QQKTARESVTPLAEMEYGKQLELKQQNSVGVVEKLYRQLIQNK------VAHLGKQTQFRDRVLREILPAPKTAGYRNKCEFTIGHDLNGEICVG--FVGGRFSKN-QHFVIPPVDVDIVSTQMMAIVGDVHE-FVKESALEPFNEFDRKGFWRMLTIREFGGDVMLIFTVFPLE-DGVERMEEIQKKIETRFLDFETFKEKKYRVCSIYWQQMV----HISDTPNFKLIGGTPYIYESLLDCRFRVSPPAFFQTNSQAAAVLY 365

HSP 2 Score: 57.3806 bits (137), Expect = 1.462e-8
Identity = 27/85 (31.76%), Postives = 46/85 (54.12%), Query Frame = 3 
Query: 1182 VVAVLNPSRDGLSTEFCETLRSLESLKMIVYISCHVEGQTTLNICTLCTVKGDSNPEVPFSLSKIIPLDPFPHTTHVEVIFQMTR 1436
            VV VL+P R G+  +     R +E+++ ++++SC      + NI  LC           FS++++ P+D FP T H+E I Q+ R
Sbjct:  520 VVGVLDPPRAGMHEKVILACREMETMRRLIFVSCEPNAAIS-NIVNLCRPSSRKYTGDAFSVTRVQPVDMFPQTNHMEWIIQLDR 603
[Close]
BLAST of tRNA (Uracil-5-)-methyltransferase-like protein vs. Ensembl Celegans
Match: trm-2A (TRna (tRNA) Methyltransferase homolog [Source:UniProtKB/TrEMBL;Acc:Q9N5L0])

HSP 1 Score: 57.3806 bits (137), Expect = 6.626e-10
Identity = 27/85 (31.76%), Postives = 46/85 (54.12%), Query Frame = 3 
Query: 1182 VVAVLNPSRDGLSTEFCETLRSLESLKMIVYISCHVEGQTTLNICTLCTVKGDSNPEVPFSLSKIIPLDPFPHTTHVEVIFQMTR 1436
            VV VL+P R G+  +     R +E+++ ++++SC      + NI  LC           FS++++ P+D FP T H+E I Q+ R
Sbjct:   51 VVGVLDPPRAGMHEKVILACREMETMRRLIFVSCEPNAAIS-NIVNLCRPSSRKYTGDAFSVTRVQPVDMFPQTNHMEWIIQLDR 134
[Close]
BLAST of tRNA (Uracil-5-)-methyltransferase-like protein vs. Ensembl Celegans
Match: trm-2A (TRna (tRNA) Methyltransferase homolog [Source:UniProtKB/TrEMBL;Acc:Q9N5L0])

HSP 1 Score: 55.0694 bits (131), Expect = 3.369e-8
Identity = 27/85 (31.76%), Postives = 46/85 (54.12%), Query Frame = 3 
Query: 1182 VVAVLNPSRDGLSTEFCETLRSLESLKMIVYISCHVEGQTTLNICTLCTVKGDSNPEVPFSLSKIIPLDPFPHTTHVEVIFQMTR 1436
            VV VL+P R G+  +     R +E+++ ++++SC      + NI  LC           FS++++ P+D FP T H+E I Q+ R
Sbjct:  202 VVGVLDPPRAGMHEKVILACREMETMRRLIFVSCEPNAAIS-NIVNLCRPSSRKYTGDAFSVTRVQPVDMFPQTNHMEWIIQLDR 285
[Close]
BLAST of tRNA (Uracil-5-)-methyltransferase-like protein vs. Ensembl Fly
Match: CG3808 (gene:FBgn0036838 transcript:FBtr0075041)

HSP 1 Score: 116.701 bits (291), Expect = 1.799e-27
Identity = 120/448 (26.79%), Postives = 213/448 (47.54%), Query Frame = 3 
Query:  129 MKKILNTTAPLLSLPYDAQLITKQYKIFSAMQNISSLLRKSNTLS-PEILEYCSKNKGLLCKIEDIYKSPVIEGYRNKMGAIVGFDEMGNLTVGHAMT-FSNFDKTRKKLVTFSHFCDKLDKKHHILIAKLFEKFIKEDSQFIP-NQKKKISLYKGITIR-SNRKNETMINIHFRARFTGIDRILGISKELSDQFLTSLQNES-INVRSLYCQQMESHFEHTHAQLSCKLIYGDKYLMENVCGKTFYISPLSFFQVNVEVAEMIYQKAKQLVEDYKSKSGKKIXXXXXXXXXXXXXIILSDIVEQVIGVDIVPSAIEXXXXXXXXXXXXXXEHFCKPVEEFLPEFLNSDIAKEYD-------NVVAVLNPSRDGLSTEFCETLRSLESLKMIVYISCHVEGQTTLNICTLCTVKGDSNPEVPFSLSKIIPLDPFPHTTHVEVIFQMTR 1436
             +  +  T PL  + YD Q+  K  ++ + +   +  LRK N  + P + ++         +  ++  SP + GYRNK    VG + +G + VG  +  +S+      ++    H  ++         A+ F+  +++ S+F+P N    I  ++ + +R S+   E M+     +     D  + + +EL   F   L N++     SLY Q ++ H E        + I G  ++ + + G  F ISPL+FFQ+N E A ++YQKA  LV   K  +   I     CCGTGT+ +  +   ++V+GV+IVP AI++A+FNA+AN + N + F    ++F+   +   +   YD       +++AV++P R GL       +RS +++  +VY+SC+       N   L   +       PF     + +D FPHT H E++    R
Sbjct:  157 QRTAVEATCPLSHIAYDQQIKQKSEEMSALLAKYTQELRKVNPRAKPHLDKF---------QFHEVLPSPTVNGYRNKNEFTVGKNSVGEVVVGFRLGCYSDGSVEVAEVADLPHLPEQAK-----WAARSFQDLVRK-SKFLPFNPDGNIGHFRQLMVRCSSATGELMLVAGIYSSNLSEDEQVELKQELK-SFYEDLANDAPYKCSSLYYQDVK-HREAGQTINPVEHISGSTHITDTIQGLQFRISPLAFFQINTEGANVLYQKAIDLVAPTKDTTMLDI-----CCGTGTITLAFAKHCKKVMGVEIVPDAIKDAEFNAEANGIKNAKFFTGNADDFIKSMVREAL---YDQEPGKPLDLIAVVDPPRAGLHHRSIAAIRSADAINRLVYVSCNPHS-AKRNFIELARPESKQYKGEPFYPKSAVAVDMFPHTMHTELVILFER 578
[Close]
BLAST of tRNA (Uracil-5-)-methyltransferase-like protein vs. Ensembl Zebrafish
Match: trmt2a (tRNA methyltransferase 2 homolog A [Source:ZFIN;Acc:ZDB-GENE-030131-6876])

HSP 1 Score: 143.28 bits (360), Expect = 2.293e-36
Identity = 123/437 (28.15%), Postives = 216/437 (49.43%), Query Frame = 3 
Query:  135 KILNTTAPLLSLPYDAQLITKQYKIFSAMQNISSLLRKSN-TLSPEILEYCSKNKGLLCKIEDIYKSPVIEGYRNKMGAIVGFDEMG-NLTVGHAMTFSNFDKTRKKLVTFSHFCDKLDKKHHILIAKLFEKFIKEDSQFIPNQKKKISLYKGITIRSNRKNETMINIHFRARFTGIDRILGISKELSDQFLTSLQNESINVRSLYCQQMESHFEHTHAQLSCKLIYGDKYLMENVCGKTFYISPLSFFQVNVEVAEMIYQKAKQLVE-DYKSKSGKKIXXXXXXXXXXXXXIILSDIVEQVIGVDIVPSAIEXXXXXXXXXXXXXXEHFCKPVEEFLPEFLNSDIAKEYDNVVAVLNPSRDGLSTEFCETLRSLESLKMIVYISCHVEGQTTLNICTLCTVKGDSNPEVPFSLSKIIPLDPFPHTTHVEVIFQMTR 1436
            +I N   PL  +PY+ QL+ K+ ++   +Q ++  +  +N  + P +     K+  + C +E I  SP+  GYRNK   ++G    G + TVG       +      +V+ S   +   +   ++  + F++FI+     + + +     ++ +T+R+ R  +TM  + F  +      +  + + L  Q+ T  + +   V SLY  +M          L C+ + G++++ E + G  F ISP SFFQ N   AE++Y    +  + D  S       +LD+CCGTGT+GI L+  V++VIG+++   A+++A+ NAKAN + NVE  C   E+  P  LN+ ++    NV A+++P R GL ++    +R  E LK +VY++C+ +     N   LC    +      F   K + +D FP T H E I    R
Sbjct:  189 QIANAVTPLWKVPYEEQLMMKEKEVEGVLQKLAREIGNNNKAMLPWLFVQKEKHNKMCCPLEAIRPSPLQTGYRNKCEFVIGVGADGEDRTVG--FRLGKYKGGSCAVVSPSETTNVCSEAKRVV--QCFQQFIRTTPYAVYSPETYEGHWRQLTVRTTRLKQTMAMVFFNPQKLQEKELEELKQNLG-QYFTEGEGKECAVTSLYFVRMGQRTSAGTEDLPCEHVTGEEWIHEELLGLKFRISPHSFFQTNTPAAEILYSAVGEWAQLDQDS------TVLDVCCGTGTIGISLAKRVKKVIGIELCQEAVKDAEANAKANGLTNVEFHCGKAEDVFPTILNAVVS---PNVTAIVDPPRAGLHSKVILAIRKAEHLKRLVYVACNAKAAMN-NFIDLCRAPSNRVRGAAFRPVKAMAVDLFPQTMHCETILLFER 610
[Close]
BLAST of tRNA (Uracil-5-)-methyltransferase-like protein vs. Ensembl Zebrafish
Match: trmt2a (tRNA methyltransferase 2 homolog A [Source:ZFIN;Acc:ZDB-GENE-030131-6876])

HSP 1 Score: 143.28 bits (360), Expect = 2.293e-36
Identity = 123/437 (28.15%), Postives = 216/437 (49.43%), Query Frame = 3 
Query:  135 KILNTTAPLLSLPYDAQLITKQYKIFSAMQNISSLLRKSN-TLSPEILEYCSKNKGLLCKIEDIYKSPVIEGYRNKMGAIVGFDEMG-NLTVGHAMTFSNFDKTRKKLVTFSHFCDKLDKKHHILIAKLFEKFIKEDSQFIPNQKKKISLYKGITIRSNRKNETMINIHFRARFTGIDRILGISKELSDQFLTSLQNESINVRSLYCQQMESHFEHTHAQLSCKLIYGDKYLMENVCGKTFYISPLSFFQVNVEVAEMIYQKAKQLVE-DYKSKSGKKIXXXXXXXXXXXXXIILSDIVEQVIGVDIVPSAIEXXXXXXXXXXXXXXEHFCKPVEEFLPEFLNSDIAKEYDNVVAVLNPSRDGLSTEFCETLRSLESLKMIVYISCHVEGQTTLNICTLCTVKGDSNPEVPFSLSKIIPLDPFPHTTHVEVIFQMTR 1436
            +I N   PL  +PY+ QL+ K+ ++   +Q ++  +  +N  + P +     K+  + C +E I  SP+  GYRNK   ++G    G + TVG       +      +V+ S   +   +   ++  + F++FI+     + + +     ++ +T+R+ R  +TM  + F  +      +  + + L  Q+ T  + +   V SLY  +M          L C+ + G++++ E + G  F ISP SFFQ N   AE++Y    +  + D  S       +LD+CCGTGT+GI L+  V++VIG+++   A+++A+ NAKAN + NVE  C   E+  P  LN+ ++    NV A+++P R GL ++    +R  E LK +VY++C+ +     N   LC    +      F   K + +D FP T H E I    R
Sbjct:  189 QIANAVTPLWKVPYEEQLMMKEKEVEGVLQKLAREIGNNNKAMLPWLFVQKEKHNKMCCPLEAIRPSPLQTGYRNKCEFVIGVGADGEDRTVG--FRLGKYKGGSCAVVSPSETTNVCSEAKRVV--QCFQQFIRTTPYAVYSPETYEGHWRQLTVRTTRLKQTMAMVFFNPQKLQEKELEELKQNLG-QYFTEGEGKECAVTSLYFVRMGQRTSAGTEDLPCEHVTGEEWIHEELLGLKFRISPHSFFQTNTPAAEILYSAVGEWAQLDQDS------TVLDVCCGTGTIGISLAKRVKKVIGIELCQEAVKDAEANAKANGLTNVEFHCGKAEDVFPTILNAVVS---PNVTAIVDPPRAGLHSKVILAIRKAEHLKRLVYVACNAKAAMN-NFIDLCRAPSNRVRGAAFRPVKAMAVDLFPQTMHCETILLFER 610
[Close]
BLAST of tRNA (Uracil-5-)-methyltransferase-like protein vs. Ensembl Zebrafish
Match: trmt2a (tRNA methyltransferase 2 homolog A [Source:ZFIN;Acc:ZDB-GENE-030131-6876])

HSP 1 Score: 140.198 bits (352), Expect = 2.538e-35
Identity = 121/437 (27.69%), Postives = 215/437 (49.20%), Query Frame = 3 
Query:  135 KILNTTAPLLSLPYDAQLITKQYKIFSAMQNISSLLRKSN-TLSPEILEYCSKNKGLLCKIEDIYKSPVIEGYRNKMGAIVGFDEMG-NLTVGHAMTFSNFDKTRKKLVTFSHFCDKLDKKHHILIAKLFEKFIKEDSQFIPNQKKKISLYKGITIRSNRKNETMINIHFRARFTGIDRILGISKELSDQFLTSLQNESINVRSLYCQQMESHFEHTHAQLSCKLIYGDKYLMENVCGKTFYISPLSFFQVNVEVAEMIYQKAKQLVE-DYKSKSGKKIXXXXXXXXXXXXXIILSDIVEQVIGVDIVPSAIEXXXXXXXXXXXXXXEHFCKPVEEFLPEFLNSDIAKEYDNVVAVLNPSRDGLSTEFCETLRSLESLKMIVYISCHVEGQTTLNICTLCTVKGDSNPEVPFSLSKIIPLDPFPHTTHVEVIFQMTR 1436
            +I N   PL  +PY+ QL+ K+ ++   +Q ++  +  +N  + P +     K+  + C +E I  SP+  GYRNK   ++G    G + TVG       +      +V+ S   +   +   ++  + F++FI+     + + +     ++ +T+R+ R  +TM  + F  +      +  + + L  Q+    + +   V SLY  +M          L C+ + G++++ E + G  F ISP SFFQ N   AE++Y    +  + D  S       +LD+CCGTGT+GI L+  V++VIG+++   A+++A+ NAKAN + NVE  C   E+  P  LN+ ++    NV A+++P R GL ++    +R  E LK +VY++C+ +     N   LC    +      F   + + +D FP T H E I    R
Sbjct:  191 QIANAVTPLWKVPYEEQLMMKEKEVEGVLQKLAREIGNNNKAMLPWLFVQKEKHNKMCCPLEAIRPSPLQTGYRNKCEFVIGVGADGEDRTVG--FRLGKYKGGSCAVVSPSETTNVCSEAKRVV--QCFQQFIRTTPYAVYSPETYEGHWRQLTVRTTRLKQTMAMVFFNPQKLQEKELEELKQNLG-QYFKEGEGKECAVTSLYFVRMGQRTSAGTEDLPCEHVTGEEWIHEELLGLKFRISPHSFFQTNTPAAEILYSAVGEWAQLDQDS------TVLDVCCGTGTIGISLAKRVKKVIGIELCQEAVKDAEANAKANGLTNVEFHCGKAEDVFPTILNAVVS---PNVTAIVDPPRAGLHSKVILAIRKAEHLKRLVYVACNAKAAMN-NFIDLCRAPSNRVRGAAFRPVRAMAVDLFPQTMHCETILLFER 612
[Close]
BLAST of tRNA (Uracil-5-)-methyltransferase-like protein vs. Ensembl Zebrafish
Match: trmt2b (tRNA methyltransferase 2 homolog B [Source:NCBI gene;Acc:606657])

HSP 1 Score: 120.553 bits (301), Expect = 3.859e-29
Identity = 133/457 (29.10%), Postives = 206/457 (45.08%), Query Frame = 3 
Query:  132 KKILNTTAPLLSLPYDAQLITKQYKIFSAMQNISSLLRKSNTLSPEILEYCSKNKGLLCKIEDIYKSPVIEGYRNK---------------MGAIVGFDEMGNLTVGHAMTFSNFDKTRKKLVTFSHFCDKLDKKHHILIAKLFEKFIKEDSQFIPNQKKKISLYKGITIRSNRKNETMINIHFRARFTGIDRILGISKELSDQFLTSLQNESINVRSLYCQQMESHFEHTHAQLSCKLIYGDKYLMENVCGKTFYISPLSFFQVNVEVAEMIYQKAKQLVEDYKSKSGKKIXXXXXXXXXXXXXIILSDIVEQVIGVDIVPSAIEXXXXXXXXXXXXXXEHFCKPVEEFLPEFLNSDIAKEYDNVVAVLNPSRDGLSTEFCETLRSLESLKMIVYISCHVEGQTTLNICTLCT-------VKGDSNPEVPFSLSKIIPLDPFPHTTHVEVIFQMTR 1436
            +++ +T  PL  + Y+ QL   Q+K +   + I  LL+    LS +     S +  L   +  I  SPV +GYRNK               +G  +G  + GN+   HA                 H      K  H ++A+ +E FI+               ++ ITIR+N    TM  ++F  +    +    I K    ++ T        + SLY Q+  +    +H Q   +L+YG  ++ E V G  F ISP SFFQVN E AE +Y+   +L +     +   +     CCGTG +GI LS  +E+VIG++++  A+E+AKFNA  N V N E      E  LP+ + S        + AV+NPSR GL       LR+  +++ +VYISC  +G+   N   LC        + G++     F     +P+D FPHT H E++    R
Sbjct:   64 ERLSDTVTPLWRMTYEDQL---QWK-YEHQKKI--LLKMMKELSQDPTRAFSDH--LNFPLLPIVASPVRDGYRNKSTFSVNKGIDGNPKTLGFYIGTGKAGNIVCVHA----------------DHLLSIPSK--HKMVARCYEDFIRLSPLRPCILFDDGGHWREITIRTNSTGHTMAIVYFHPQSLTPEET-DIHKAALVEYFTQGPGAICQLDSLYFQET-TMTRCSHEQSQYQLLYGQTHIYEEVLGFKFRISPDSFFQVNREAAEALYKTVAELSQPCVGGTLLDV-----CCGTGAIGISLSPQMERVIGIELIEQAVEDAKFNAALNRVCNCEFLAGKAEVVLPDLMGS--LSSDGGLTAVVNPSRAGLHYRVVRALRNHSAIRRLVYISCKPDGEAMRNFRELCCSVGLVRRITGEA-----FKPVVAVPVDLFPHTPHCELVLVFER 480
[Close]
BLAST of tRNA (Uracil-5-)-methyltransferase-like protein vs. Ensembl Zebrafish
Match: trmt2b (tRNA methyltransferase 2 homolog B [Source:NCBI gene;Acc:606657])

HSP 1 Score: 120.553 bits (301), Expect = 4.433e-29
Identity = 133/457 (29.10%), Postives = 206/457 (45.08%), Query Frame = 3 
Query:  132 KKILNTTAPLLSLPYDAQLITKQYKIFSAMQNISSLLRKSNTLSPEILEYCSKNKGLLCKIEDIYKSPVIEGYRNK---------------MGAIVGFDEMGNLTVGHAMTFSNFDKTRKKLVTFSHFCDKLDKKHHILIAKLFEKFIKEDSQFIPNQKKKISLYKGITIRSNRKNETMINIHFRARFTGIDRILGISKELSDQFLTSLQNESINVRSLYCQQMESHFEHTHAQLSCKLIYGDKYLMENVCGKTFYISPLSFFQVNVEVAEMIYQKAKQLVEDYKSKSGKKIXXXXXXXXXXXXXIILSDIVEQVIGVDIVPSAIEXXXXXXXXXXXXXXEHFCKPVEEFLPEFLNSDIAKEYDNVVAVLNPSRDGLSTEFCETLRSLESLKMIVYISCHVEGQTTLNICTLCT-------VKGDSNPEVPFSLSKIIPLDPFPHTTHVEVIFQMTR 1436
            +++ +T  PL  + Y+ QL   Q+K +   + I  LL+    LS +     S +  L   +  I  SPV +GYRNK               +G  +G  + GN+   HA                 H      K  H ++A+ +E FI+               ++ ITIR+N    TM  ++F  +    +    I K    ++ T        + SLY Q+  +    +H Q   +L+YG  ++ E V G  F ISP SFFQVN E AE +Y+   +L +     +   +     CCGTG +GI LS  +E+VIG++++  A+E+AKFNA  N V N E      E  LP+ + S        + AV+NPSR GL       LR+  +++ +VYISC  +G+   N   LC        + G++     F     +P+D FPHT H E++    R
Sbjct:   57 ERLSDTVTPLWRMTYEDQL---QWK-YEHQKKI--LLKMMKELSQDPTRAFSDH--LNFPLLPIVASPVRDGYRNKSTFSVNKGIDGNPKTLGFYIGTGKAGNIVCVHA----------------DHLLSIPSK--HKMVARCYEDFIRLSPLRPCILFDDGGHWREITIRTNSTGHTMAIVYFHPQSLTPEET-DIHKAALVEYFTQGPGAICQLDSLYFQET-TMTRCSHEQSQYQLLYGQTHIYEEVLGFKFRISPDSFFQVNREAAEALYKTVAELSQPCVGGTLLDV-----CCGTGAIGISLSPQMERVIGIELIEQAVEDAKFNAALNRVCNCEFLAGKAEVVLPDLMGS--LSSDGGLTAVVNPSRAGLHYRVVRALRNHSAIRRLVYISCKPDGEAMRNFRELCCSVGLVRRITGEA-----FKPVVAVPVDLFPHTPHCELVLVFER 473
[Close]
BLAST of tRNA (Uracil-5-)-methyltransferase-like protein vs. Ensembl Xenopus
Match: TRMT2B (tRNA methyltransferase 2 homolog B [Source:NCBI gene;Acc:100495482])

HSP 1 Score: 134.806 bits (338), Expect = 3.702e-34
Identity = 135/451 (29.93%), Postives = 215/451 (47.67%), Query Frame = 3 
Query:  132 KKILNTTAPLLSLPYDAQLITKQYKIFSAMQNISSLLRKSNTLSPEILEYCSKNKGLLCKIEDIYKSPVIEGYRNKMGAIVGFDEMGNLTVGHAMTFSNFDKTRKK----LVTFSHFCDKLDKKHHILIAKLFEKFIKEDSQFIPNQKKKISLYKG-----ITIRSNRKNETMINIHFRARFTGIDRILGISKELSDQFLTSLQNESINVRSLYCQQMESHFEHTHAQLSCKLIYGDKYLMENVCGKTFYISPLSFFQVNVEVAEMIYQKAKQLVEDYKSKSGKKIXXXXXXXXXXXXXIILSDIVEQVIGVDIVPSAIEXXXXXXXXXXXXXXEHFCKPVEEFLPEFLNSDIAKEYDNVVAVLNPSRDGLSTEFCETLRSLESLKMIVYISCHVEGQTTLNICTLCTVK-------GDSNPEVPFSLSKIIPLDPFPHTTHVEVIFQMTR 1436
            +++ +   PL  + Y  QL  K   + S +++I+  +R       ++++ C    GL C ++DI  SPV+ GYRNK    V     G+       T  NF  T +      V   H  +  +K  H+  A+ +E FI + S   P     +  Y G     + +R+N+K ETM  + F  +    + +L   + L D F T       N+ SLY Q+       TH     +L+YG  +L E V      +SP SF QVN   AE +Y     +++ +   +     LLDLCCGTG +G+ L+   + VIGV++V  A+ +A++NA  N + N +      E+ LP  L S   KE  +V+AV NP R GL       LR+ E ++ +++++C  EG+ T N+  LC          G++     F   + + +D FPHTTH E+   +TR
Sbjct:   18 ERLSDVATPLWRMGYKEQLEYKLNALRSTLRDITIHVRARGG---KVVD-CD---GLFCPLQDIIPSPVMNGYRNKSSFSVNRGPDGD-----PKTVGNFVGTGRDRNIVCVRADHLINIPEKHKHV--ARCYEDFIHQ-SPLAPC----LLFYDGGHWRDVVVRTNKKGETMAIVTFHPQDLPQNEVLSHIQSLKDYF-TQGPGSECNLTSLYYQENNPK---THQPSPPRLLYGSPHLTEQVLDLRLRVSPTSFLQVNTPAAETLYTALGNMIQRHAHGT-----LLDLCCGTGAIGLSLAGYFKNVIGVEMVEDAVLDARWNADFNGIENCQFLSGRAEKLLPNLL-SYKQKEL-SVLAVANPPRAGLHPRVVRALRNCEDIRTLLFVTCKPEGEATRNLTELCCAPSLEQKILGEA-----FVPQEALGVDLFPHTTHCELALLLTR 433
[Close]
BLAST of tRNA (Uracil-5-)-methyltransferase-like protein vs. Ensembl Xenopus
Match: TRMT2B (tRNA methyltransferase 2 homolog B [Source:NCBI gene;Acc:100495482])

HSP 1 Score: 130.568 bits (327), Expect = 6.325e-33
Identity = 125/396 (31.57%), Postives = 190/396 (47.98%), Query Frame = 3 
Query:  297 GLLCKIEDIYKSPVIEGYRNKMGAIVGFDEMGNLTVGHAMTFSNFDKTRKK----LVTFSHFCDKLDKKHHILIAKLFEKFIKEDSQFIPNQKKKISLYKG-----ITIRSNRKNETMINIHFRARFTGIDRILGISKELSDQFLTSLQNESINVRSLYCQQMESHFEHTHAQLSCKLIYGDKYLMENVCGKTFYISPLSFFQVNVEVAEMIYQKAKQLVEDYKSKSGKKIXXXXXXXXXXXXXIILSDIVEQVIGVDIVPSAIEXXXXXXXXXXXXXXEHFCKPVEEFLPEFLNSDIAKEYDNVVAVLNPSRDGLSTEFCETLRSLESLKMIVYISCHVEGQTTLNICTLCTVK-------GDSNPEVPFSLSKIIPLDPFPHTTHVEVIFQMTR 1436
            GL C ++DI  SPV+ GYRNK    V     G+       T  NF  T +      V   H  +  +K  H+  A+ +E FI + S   P     +  Y G     + +R+N+K ETM  + F  +    + +L   + L D F T       N+ SLY Q+       TH     +L+YG  +L E V      +SP SF QVN   AE +Y     +++ +   +     LLDLCCGTG +G+ L+   + VIGV++V  A+ +A++NA  N + N +      E+ LP  L S   KE  +V+AV NP R GL       LR+ E ++ +++++C  EG+ T N+  LC          G++     F   + + +D FPHTTH E+   +TR
Sbjct:   37 GLFCPLQDIIPSPVMNGYRNKSSFSVNRGPDGD-----PKTVGNFVGTGRDRNIVCVRADHLINIPEKHKHV--ARCYEDFIHQ-SPLAPC----LLFYDGGHWRDVVVRTNKKGETMAIVTFHPQDLPQNEVLSHIQSLKDYF-TQGPGSECNLTSLYYQENNPK---THQPSPPRLLYGSPHLTEQVLDLRLRVSPTSFLQVNTPAAETLYTALGNMIQRHAHGT-----LLDLCCGTGAIGLSLAGYFKNVIGVEMVEDAVLDARWNADFNGIENCQFLSGRAEKLLPNLL-SYKQKEL-SVLAVANPPRAGLHPRVVRALRNCEDIRTLLFVTCKPEGEATRNLTELCCAPSLEQKILGEA-----FVPQEALGVDLFPHTTHCELALLLTR 404
[Close]
BLAST of tRNA (Uracil-5-)-methyltransferase-like protein vs. Ensembl Xenopus
Match: hspa12b (heat shock protein family A (Hsp70) member 12B [Source:Xenbase;Acc:XB-GENE-1003961])

HSP 1 Score: 128.642 bits (322), Expect = 1.968e-31
Identity = 127/457 (27.79%), Postives = 219/457 (47.92%), Query Frame = 3 
Query:  132 KKILNTTAPLLSLPYDAQLITKQYKIFSAMQNISSLLRKSN-TLSPEILEYCSKNKGLLCKIEDIYKSPVIEGYRNKMGAIVGFD----------EMGNLTVGHAMTFSNFDKTRKKLVTFSHFCDKLDKKHHILIAKLFEKFIKEDSQFIPNQKKKISLYKGITIRSNRKNETMINIHFRARFTGIDRILGISKELSDQFLTSLQNESINVRSLY----CQQMESHFEHTHAQLSCKLIYGDKYLMENVCGKTFYISPLSFFQVNVEVAEMIYQKAKQLVEDYKSKSGKKIXXXXXXXXXXXXXIILSDIVEQVIGVDIVPSAIEXXXXXXXXXXXXXXEHFCKPVEEFLPEFLNSDIAKEYDNVVAVLNPSRDGLSTEFCETLRSLESLKMIVYISCHVEGQTTLNICTLCTVKGDSNPEVPFSLSKIIPLDPFPHTTHVEVIFQMTR*NINKIV 1457
            K I +   PL  +PY+ QL  K+ +    ++ ++  +  +N  L+P + E   K   L C +E +  SP+   YRNK   ++G+            +     G       FD      VT +             + + F+++I+     + + +     +K +T+R++RK + M  ++F  +    + +  +   LS  F      ES  V SLY     Q+   H E     L  + I GD+Y+ E++ G TF ISP +FFQVN   AE++Y      + D+ ++  +   +LD+CCGTGT+GI L+  V++V+G+++   AI +AK NA+ NN++NVE  C   E+  P  + S     + + +A+++P R GL ++    +R  E LK ++Y+SC+ +     N   LC    +     PF   K + +D FP T H E++    R    +IV
Sbjct:  128 KTISDVVTPLWQVPYEEQLKIKEEECCKVLKQLTKEIGVNNKPLAPWLFEQKQKYNRLCCPLERVIPSPIQTEYRNKSEFLIGYGVNQEDKTVGCRLSKYKGGTCAVVEPFDTINIPPVTKN-------------VVRAFQEYIRSTPYDVYSPETYAGHWKQLTVRTSRKGQVMAIVYFNPQKLPKEDLSELKTSLSMYFTEGSGKES-GVTSLYFVEEGQRKSPHLE----DLPVEHIAGDQYIYEDLLGLTFRISPHAFFQVNTPAAEVLYSA----IADW-AQLDQNTTVLDVCCGTGTIGISLAKKVKKVVGIELCQEAIADAKANAQLNNLDNVEFRCGKAEDIFPTLIYSFT---FPSPLAIVDPPRAGLHSKVIIAIRKAEHLKQLIYVSCNPKAAMN-NFIDLCRAPSNRVKGRPFRPIKALAVDLFPQTPHFELVILFERTEPAEIV 557
[Close]
BLAST of tRNA (Uracil-5-)-methyltransferase-like protein vs. Ensembl Xenopus
Match: hspa12b (heat shock protein family A (Hsp70) member 12B [Source:Xenbase;Acc:XB-GENE-1003961])

HSP 1 Score: 128.642 bits (322), Expect = 2.024e-31
Identity = 127/457 (27.79%), Postives = 219/457 (47.92%), Query Frame = 3 
Query:  132 KKILNTTAPLLSLPYDAQLITKQYKIFSAMQNISSLLRKSN-TLSPEILEYCSKNKGLLCKIEDIYKSPVIEGYRNKMGAIVGFD----------EMGNLTVGHAMTFSNFDKTRKKLVTFSHFCDKLDKKHHILIAKLFEKFIKEDSQFIPNQKKKISLYKGITIRSNRKNETMINIHFRARFTGIDRILGISKELSDQFLTSLQNESINVRSLY----CQQMESHFEHTHAQLSCKLIYGDKYLMENVCGKTFYISPLSFFQVNVEVAEMIYQKAKQLVEDYKSKSGKKIXXXXXXXXXXXXXIILSDIVEQVIGVDIVPSAIEXXXXXXXXXXXXXXEHFCKPVEEFLPEFLNSDIAKEYDNVVAVLNPSRDGLSTEFCETLRSLESLKMIVYISCHVEGQTTLNICTLCTVKGDSNPEVPFSLSKIIPLDPFPHTTHVEVIFQMTR*NINKIV 1457
            K I +   PL  +PY+ QL  K+ +    ++ ++  +  +N  L+P + E   K   L C +E +  SP+   YRNK   ++G+            +     G       FD      VT +             + + F+++I+     + + +     +K +T+R++RK + M  ++F  +    + +  +   LS  F      ES  V SLY     Q+   H E     L  + I GD+Y+ E++ G TF ISP +FFQVN   AE++Y      + D+ ++  +   +LD+CCGTGT+GI L+  V++V+G+++   AI +AK NA+ NN++NVE  C   E+  P  + S     + + +A+++P R GL ++    +R  E LK ++Y+SC+ +     N   LC    +     PF   K + +D FP T H E++    R    +IV
Sbjct:  128 KTISDVVTPLWQVPYEEQLKIKEEECCKVLKQLTKEIGVNNKPLAPWLFEQKQKYNRLCCPLERVIPSPIQTEYRNKSEFLIGYGVNQEDKTVGCRLSKYKGGTCAVVEPFDTINIPPVTKN-------------VVRAFQEYIRSTPYDVYSPETYAGHWKQLTVRTSRKGQVMAIVYFNPQKLPKEDLSELKTSLSMYFTEGSGKES-GVTSLYFVEEGQRKSPHLE----DLPVEHIAGDQYIYEDLLGLTFRISPHAFFQVNTPAAEVLYSA----IADW-AQLDQNTTVLDVCCGTGTIGISLAKKVKKVVGIELCQEAIADAKANAQLNNLDNVEFRCGKAEDIFPTLIYSFT---FPSPLAIVDPPRAGLHSKVIIAIRKAEHLKQLIYVSCNPKAAMN-NFIDLCRAPSNRVKGRPFRPIKALAVDLFPQTPHFELVILFERTEPAEIV 557
[Close]
BLAST of tRNA (Uracil-5-)-methyltransferase-like protein vs. Ensembl Xenopus
Match: hspa12b (heat shock protein family A (Hsp70) member 12B [Source:Xenbase;Acc:XB-GENE-1003961])

HSP 1 Score: 129.028 bits (323), Expect = 2.026e-31
Identity = 127/458 (27.73%), Postives = 219/458 (47.82%), Query Frame = 3 
Query:  132 KKILNTTAPLLSLPYDAQLITKQYKIFSAMQNISSLLRKSN-TLSPEILEYCSKNKGLLCKIEDIYKSPVIEGYRNKMGAIVGFD----------EMGNLTVGHAMTFSNFDKTRKKLVTFSHFCDKLDKKHHILIAKLFEKFIKEDSQFIPNQKKKISLYKGITIRSNRKNETMINIHFRARFTGIDRILGISKELSDQFLTSLQNESINVRSLY----CQQMESHFEHTHAQLSCKLIYGDKYLMENVCGKTFYISPLSFFQVNVEVAEMIYQKAKQLVEDYKSKSGKKIXXXXXXXXXXXXXIILSDIVEQVIGVDIVPSAIEXXXXXXXXXXXXXXEHFCKPVEEFLPEFLNSDIAKEYDNVVAVLNPSRDGLSTEFCETLRSLESLKMIVYISCHVEGQTTLNICTLCTVKGDSNPEVPFSLSKIIPLDPFPHTTHVEVIFQMTR*NINKIVV 1460
            K I +   PL  +PY+ QL  K+ +    ++ ++  +  +N  L+P + E   K   L C +E +  SP+   YRNK   ++G+            +     G       FD      VT +             + + F+++I+     + + +     +K +T+R++RK + M  ++F  +    + +  +   LS  F      ES  V SLY     Q+   H E     L  + I GD+Y+ E++ G TF ISP +FFQVN   AE++Y      + D+ ++  +   +LD+CCGTGT+GI L+  V++V+G+++   AI +AK NA+ NN++NVE  C   E+  P  + S     + + +A+++P R GL ++    +R  E LK ++Y+SC+ +     N   LC    +     PF   K + +D FP T H E++    R    +IV 
Sbjct:  139 KTISDVVTPLWQVPYEEQLKIKEEECCKVLKQLTKEIGVNNKPLAPWLFEQKQKYNRLCCPLERVIPSPIQTEYRNKSEFLIGYGVNQEDKTVGCRLSKYKGGTCAVVEPFDTINIPPVTKN-------------VVRAFQEYIRSTPYDVYSPETYAGHWKQLTVRTSRKGQVMAIVYFNPQKLPKEDLSELKTSLSMYFTEGSGKES-GVTSLYFVEEGQRKSPHLE----DLPVEHIAGDQYIYEDLLGLTFRISPHAFFQVNTPAAEVLY----SAIADW-AQLDQNTTVLDVCCGTGTIGISLAKKVKKVVGIELCQEAIADAKANAQLNNLDNVEFRCGKAEDIFPTLIYSFT---FPSPLAIVDPPRAGLHSKVIIAIRKAEHLKQLIYVSCNPKAAMN-NFIDLCRAPSNRVKGRPFRPIKALAVDLFPQTPHFELVILFERTEPAEIVT 569
[Close]
BLAST of tRNA (Uracil-5-)-methyltransferase-like protein vs. Ensembl Mouse
Match: Trmt2b (TRM2 tRNA methyltransferase 2B [Source:MGI Symbol;Acc:MGI:2442530])

HSP 1 Score: 137.502 bits (345), Expect = 6.280e-35
Identity = 127/442 (28.73%), Postives = 209/442 (47.29%), Query Frame = 3 
Query:  132 KKILNTTAPLLSLPYDAQLITKQYKIFSAMQNISSLLRKSNTLSPEILEYCSKNKGLLCKIEDIYKSPVIEGYRNKMGAIVGFDEMGNL-TVGHAMTFSNFDKTRKKLVTFSHFCDKLDK--KHHILIAKLFEKFIKEDSQFIPNQKKKISLYKGITIRSNRKNETMINIHFRARFTGIDRILGISKELSDQFLTSLQNESINVRSLYCQQMESHFEHTHAQLSCKLIYGDKYLMENVCGKTFYISPLSFFQVNVEVAEMIYQKAKQLVEDYKSKSGKKIXXXXXXXXXXXXXIILS--DIVEQVIGVDIVPSAIEXXXXXXXXXXXXXXEHFCKPVEEFLPEFLNSDIAKEYDNVVAVLNPSRDGLSTEFCETLRSLESLKMIVYISCHVEGQTTLNICTLCTVKGDSNPEV--PFSLSKIIPLDPFPHTTHVEVIFQMTR 1436
            +++ +   PL  L Y+AQL  K       +Q++ S L+  + +S  +  +  +++GL C +  I  SP   GYRNK    V     GN  TVG+ +       T K        C+ L    + H  +A+ +E F+++ S        +   ++ + +R+NR+  TM  + F  +    + +      L D F T        + SLY Q+  +    +H Q   +L++G+ ++ E++ G    ISP +FFQ+N   AEM+Y+   +L       SG     L L    GT  I LS      QV G+++V  A+E+A++ A  N V N E      E  LP+ L S   K+    VAV+NP+R GL       +R+  ++  +V++SC   G++T N   LC     +   +  PF L + +P+D FPHT H E++   TR
Sbjct:   71 ERLADVVTPLWRLSYEAQLKVKFEAQKKLLQSLESHLKVLHGVSDTVAAH--QSEGLRCLLHPIIPSPTTTGYRNKSTFSVYRSPDGNPKTVGYYL------GTWKDGNVVCLPCNHLKNIPEKHSQVAQYYEVFLRQSSVEPCLLFHEGGHWRELVVRTNRQGHTMAIVTFHPQGLSEEEVCVQKVTLKD-FFTKGPGAICELTSLYFQE-STMTRCSHQQSPYQLLFGEPHIFEDLLGLKIRISPDAFFQINTAGAEMLYRIIGEL-------SGVNSESLLLDICCGTGVIGLSVAQRASQVHGIELVEQAVEDARWTAAFNGVTNCEFHAGRAETILPQLLKSQ--KDEKLTVAVVNPARAGLHYRVVRAIRNCRTIHTLVFVSCKPHGESTRNFIELCCPPNSAKQLLGDPFVLREAVPVDLFPHTPHCELVLLFTR 493
[Close]
BLAST of tRNA (Uracil-5-)-methyltransferase-like protein vs. Ensembl Mouse
Match: Trmt2b (TRM2 tRNA methyltransferase 2B [Source:MGI Symbol;Acc:MGI:2442530])

HSP 1 Score: 137.502 bits (345), Expect = 6.280e-35
Identity = 127/442 (28.73%), Postives = 209/442 (47.29%), Query Frame = 3 
Query:  132 KKILNTTAPLLSLPYDAQLITKQYKIFSAMQNISSLLRKSNTLSPEILEYCSKNKGLLCKIEDIYKSPVIEGYRNKMGAIVGFDEMGNL-TVGHAMTFSNFDKTRKKLVTFSHFCDKLDK--KHHILIAKLFEKFIKEDSQFIPNQKKKISLYKGITIRSNRKNETMINIHFRARFTGIDRILGISKELSDQFLTSLQNESINVRSLYCQQMESHFEHTHAQLSCKLIYGDKYLMENVCGKTFYISPLSFFQVNVEVAEMIYQKAKQLVEDYKSKSGKKIXXXXXXXXXXXXXIILS--DIVEQVIGVDIVPSAIEXXXXXXXXXXXXXXEHFCKPVEEFLPEFLNSDIAKEYDNVVAVLNPSRDGLSTEFCETLRSLESLKMIVYISCHVEGQTTLNICTLCTVKGDSNPEV--PFSLSKIIPLDPFPHTTHVEVIFQMTR 1436
            +++ +   PL  L Y+AQL  K       +Q++ S L+  + +S  +  +  +++GL C +  I  SP   GYRNK    V     GN  TVG+ +       T K        C+ L    + H  +A+ +E F+++ S        +   ++ + +R+NR+  TM  + F  +    + +      L D F T        + SLY Q+  +    +H Q   +L++G+ ++ E++ G    ISP +FFQ+N   AEM+Y+   +L       SG     L L    GT  I LS      QV G+++V  A+E+A++ A  N V N E      E  LP+ L S   K+    VAV+NP+R GL       +R+  ++  +V++SC   G++T N   LC     +   +  PF L + +P+D FPHT H E++   TR
Sbjct:   71 ERLADVVTPLWRLSYEAQLKVKFEAQKKLLQSLESHLKVLHGVSDTVAAH--QSEGLRCLLHPIIPSPTTTGYRNKSTFSVYRSPDGNPKTVGYYL------GTWKDGNVVCLPCNHLKNIPEKHSQVAQYYEVFLRQSSVEPCLLFHEGGHWRELVVRTNRQGHTMAIVTFHPQGLSEEEVCVQKVTLKD-FFTKGPGAICELTSLYFQE-STMTRCSHQQSPYQLLFGEPHIFEDLLGLKIRISPDAFFQINTAGAEMLYRIIGEL-------SGVNSESLLLDICCGTGVIGLSVAQRASQVHGIELVEQAVEDARWTAAFNGVTNCEFHAGRAETILPQLLKSQ--KDEKLTVAVVNPARAGLHYRVVRAIRNCRTIHTLVFVSCKPHGESTRNFIELCCPPNSAKQLLGDPFVLREAVPVDLFPHTPHCELVLLFTR 493
[Close]
BLAST of tRNA (Uracil-5-)-methyltransferase-like protein vs. Ensembl Mouse
Match: Trmt2a (TRM2 tRNA methyltransferase 2A [Source:MGI Symbol;Acc:MGI:96270])

HSP 1 Score: 125.561 bits (314), Expect = 2.611e-30
Identity = 116/445 (26.07%), Postives = 210/445 (47.19%), Query Frame = 3 
Query:  135 KILNTTAPLLSLPYDAQLITKQYKIFSAMQNISSLLRKSN-TLSPEILEYCSKNKGLLCKIEDIYKSPVIEGYRNKMGAIVGFD----------EMGNLTVGHAMTFSNFDKTRKKLVTFSHFCDKLDKKHHILIAKLFEKFIKEDSQFIPNQKKKISLYKGITIRSNRKNETMINIHFRARFTGIDRILGISKELSDQFLTSLQNESINVRSLYCQQMESHFEHTHAQLSCKLIYGDKYLMENVCGKTFYISPLSFFQVNVEVAEMIYQKAKQLVEDYKSKSGKKIXXXXXXXXXXXXXIILSDIVEQVIGVDIVPSAIEXXXXXXXXXXXXXXEHFCKPVEEFLPEFLNSDIAKEYDNVVAVLNPSRDGLSTEFCETLRSLESLKMIVYISCHVEGQTTLNICTLCTVKGDSNPEVPFSLSKIIPLDPFPHTTHVEVIFQMTR 1436
            +I +   PL ++PY  QL  K+ +    +Q ++  +  +N  L P +L    ++    C +E +  SP    YRNK   +VG             +G    G     + FD          H  +   +     + K F++FI+       + +     +K +T+R++ + + M   +F  +    + + G+   L   F+      S  V SLY  +       +   L  + + GD+ + E++ G TF ISP +FFQVN   AE++Y     +++++    G    +LD+CCGTGT+G+ L+  V++V+G+++   A+E+A+ NA  N ++NVE  C   E+ +P  ++   + +   +VAVL+P R GL ++    +R  E++K ++Y+SC+       N   LC    +     PF   K + +D FP T H E++    R
Sbjct:  157 QIADVVTPLWTVPYTEQLEQKRLECERVLQKLAKEIGNTNRALLPWLLLQRQQHNKACCPLEGVKPSPQQTEYRNKCEFLVGVGVDGKDNTVGCRLGKYKGGTCAVAAPFDTV--------HIPEATKQ-----VVKAFQEFIRSTPYSAYDPETYTGHWKQLTVRTSSRGQAMAIAYFHPQKLSSEEVAGLKASLVCHFMEGPGKAS-GVTSLYFVEEGQRKTPSQEGLPLEHMAGDQCIQEDLLGLTFRISPHAFFQVNTPAAEVLY----TVIQEWAQLDGGST-VLDVCCGTGTIGLALAPKVKRVVGIELCQEAVEDARMNALTNELSNVEFHCGRAEDLVPGLVSRLSSHQ---LVAVLDPPRAGLHSKVILAIRKAENIKRLLYVSCNPRAAMG-NFVDLCRAPSNRVKGTPFHPVKAVAVDLFPQTPHCEMLILFER 578
[Close]
BLAST of tRNA (Uracil-5-)-methyltransferase-like protein vs. Ensembl Mouse
Match: Trmt2a (TRM2 tRNA methyltransferase 2A [Source:MGI Symbol;Acc:MGI:96270])

HSP 1 Score: 125.561 bits (314), Expect = 2.611e-30
Identity = 116/445 (26.07%), Postives = 210/445 (47.19%), Query Frame = 3 
Query:  135 KILNTTAPLLSLPYDAQLITKQYKIFSAMQNISSLLRKSN-TLSPEILEYCSKNKGLLCKIEDIYKSPVIEGYRNKMGAIVGFD----------EMGNLTVGHAMTFSNFDKTRKKLVTFSHFCDKLDKKHHILIAKLFEKFIKEDSQFIPNQKKKISLYKGITIRSNRKNETMINIHFRARFTGIDRILGISKELSDQFLTSLQNESINVRSLYCQQMESHFEHTHAQLSCKLIYGDKYLMENVCGKTFYISPLSFFQVNVEVAEMIYQKAKQLVEDYKSKSGKKIXXXXXXXXXXXXXIILSDIVEQVIGVDIVPSAIEXXXXXXXXXXXXXXEHFCKPVEEFLPEFLNSDIAKEYDNVVAVLNPSRDGLSTEFCETLRSLESLKMIVYISCHVEGQTTLNICTLCTVKGDSNPEVPFSLSKIIPLDPFPHTTHVEVIFQMTR 1436
            +I +   PL ++PY  QL  K+ +    +Q ++  +  +N  L P +L    ++    C +E +  SP    YRNK   +VG             +G    G     + FD          H  +   +     + K F++FI+       + +     +K +T+R++ + + M   +F  +    + + G+   L   F+      S  V SLY  +       +   L  + + GD+ + E++ G TF ISP +FFQVN   AE++Y     +++++    G    +LD+CCGTGT+G+ L+  V++V+G+++   A+E+A+ NA  N ++NVE  C   E+ +P  ++   + +   +VAVL+P R GL ++    +R  E++K ++Y+SC+       N   LC    +     PF   K + +D FP T H E++    R
Sbjct:  157 QIADVVTPLWTVPYTEQLEQKRLECERVLQKLAKEIGNTNRALLPWLLLQRQQHNKACCPLEGVKPSPQQTEYRNKCEFLVGVGVDGKDNTVGCRLGKYKGGTCAVAAPFDTV--------HIPEATKQ-----VVKAFQEFIRSTPYSAYDPETYTGHWKQLTVRTSSRGQAMAIAYFHPQKLSSEEVAGLKASLVCHFMEGPGKAS-GVTSLYFVEEGQRKTPSQEGLPLEHMAGDQCIQEDLLGLTFRISPHAFFQVNTPAAEVLY----TVIQEWAQLDGGST-VLDVCCGTGTIGLALAPKVKRVVGIELCQEAVEDARMNALTNELSNVEFHCGRAEDLVPGLVSRLSSHQ---LVAVLDPPRAGLHSKVILAIRKAENIKRLLYVSCNPRAAMG-NFVDLCRAPSNRVKGTPFHPVKAVAVDLFPQTPHCEMLILFER 578
[Close]
BLAST of tRNA (Uracil-5-)-methyltransferase-like protein vs. Ensembl Mouse
Match: Trmt2a (TRM2 tRNA methyltransferase 2A [Source:MGI Symbol;Acc:MGI:96270])

HSP 1 Score: 125.176 bits (313), Expect = 3.589e-30
Identity = 116/445 (26.07%), Postives = 210/445 (47.19%), Query Frame = 3 
Query:  135 KILNTTAPLLSLPYDAQLITKQYKIFSAMQNISSLLRKSN-TLSPEILEYCSKNKGLLCKIEDIYKSPVIEGYRNKMGAIVGFD----------EMGNLTVGHAMTFSNFDKTRKKLVTFSHFCDKLDKKHHILIAKLFEKFIKEDSQFIPNQKKKISLYKGITIRSNRKNETMINIHFRARFTGIDRILGISKELSDQFLTSLQNESINVRSLYCQQMESHFEHTHAQLSCKLIYGDKYLMENVCGKTFYISPLSFFQVNVEVAEMIYQKAKQLVEDYKSKSGKKIXXXXXXXXXXXXXIILSDIVEQVIGVDIVPSAIEXXXXXXXXXXXXXXEHFCKPVEEFLPEFLNSDIAKEYDNVVAVLNPSRDGLSTEFCETLRSLESLKMIVYISCHVEGQTTLNICTLCTVKGDSNPEVPFSLSKIIPLDPFPHTTHVEVIFQMTR 1436
            +I +   PL ++PY  QL  K+ +    +Q ++  +  +N  L P +L    ++    C +E +  SP    YRNK   +VG             +G    G     + FD          H  +   +     + K F++FI+       + +     +K +T+R++ + + M   +F  +    + + G+   L   F+      S  V SLY  +       +   L  + + GD+ + E++ G TF ISP +FFQVN   AE++Y     +++++    G    +LD+CCGTGT+G+ L+  V++V+G+++   A+E+A+ NA  N ++NVE  C   E+ +P  ++   + +   +VAVL+P R GL ++    +R  E++K ++Y+SC+       N   LC    +     PF   K + +D FP T H E++    R
Sbjct:  157 QIADVVTPLWTVPYTEQLEQKRLECERVLQKLAKEIGNTNRALLPWLLLQRQQHNKACCPLEGVKPSPQQTEYRNKCEFLVGVGVDGKDNTVGCRLGKYKGGTCAVAAPFDTV--------HIPEATKQ-----VVKAFQEFIRSTPYSAYDPETYTGHWKQLTVRTSSRGQAMAIAYFHPQKLSSEEVAGLKASLVCHFMEGPGKAS-GVTSLYFVEEGQRKTPSQEGLPLEHMAGDQCIQEDLLGLTFRISPHAFFQVNTPAAEVLY----TVIQEWAQLDGGST-VLDVCCGTGTIGLALAPKVKRVVGIELCQEAVEDARMNALTNELSNVEFHCGRAEDLVPGLVSRLSSHQ---LVAVLDPPRAGLHSKVILAIRKAENIKRLLYVSCNPRAAMG-NFVDLCRAPSNRVKGTPFHPVKAVAVDLFPQTPHCEMLILFER 578
[Close]
BLAST of tRNA (Uracil-5-)-methyltransferase-like protein vs. UniProt/SwissProt
Match: sp|Q5RFM7|TRM2_PONAB (tRNA (uracil(54)-C(5))-methyltransferase homolog OS=Pongo abelii OX=9601 GN=TRMT2B PE=2 SV=1)

HSP 1 Score: 146.362 bits (368), Expect = 3.189e-37
Identity = 125/438 (28.54%), Postives = 222/438 (50.68%), Query Frame = 3 
Query:  132 KKILNTTAPLLSLPYDAQLITKQYKIFSAMQNISSLLRKSNTLSPEILEYCSKNKGLLCKIEDIYKSPVIEGYRNKMGAIVGFDEMGNL-TVGHAMTFSNFDKTRKKLVTFSHFCDKLDKKHHILIAKLFEKFIKEDSQFIPNQKKKISLYKGITIRSNRKNETMINIHFRARFTGIDRILGISKELSDQFLTSLQNESINVRSLYCQQMESHFEHTHAQLSCKLIYGDKYLMENVCGKTFYISPLSFFQVNVEVAEMIYQKAKQLVEDYKSKSGKKIXXXXXXXXXXXXXIILSDIVEQVIGVDIVPSAIEXXXXXXXXXXXXXXEHFCKPVEEFLPEFLNSDIAKEYDNVVAVLNPSRDGLSTEFCETLRSLESLKMIVYISCHVEGQTTLNICTLCTVKGDSNPEV--PFSLSKIIPLDPFPHTTHVEVIFQMTR 1436
            +++ +   PL  L Y+ QL  K       +Q + S ++  N +S  +     K++ L C +  I  SPVI GYRNK    V     GN  TVG  +    +       V  +H  +  +K  H  +A+ +E F+++          +   ++ +T+R+N +  TM  I F  +    +    + KE+  +F       + ++ SLY Q+  +    +H Q   +L++G+ Y+ E +      ISP +FFQ+N   AEM+Y+   +L     +      +LLD+CCGTG +G+ LS    +V+G+++V  A+E+A++ A  N + N E      E+ LP  L S   ++  ++VAV+NP+R GL  +  + +R+  ++  +V++SC + G++T N+  LC     +   +  PF L +++P+D FPHT H E++   TR
Sbjct:   64 ERLADVVTPLWRLSYEEQLKVKFAAQKKILQRLESYIQMLNGVS--VTTAVPKSERLSCLLHPIIPSPVINGYRNKSTFSVNRGPDGNPKTVGFYL--GTWRDGNMVCVQSNHLKNIPEK--HSQVAQYYEVFLRQSPLEPCLVFHEGGYWRELTVRTNSQGHTMAIITFHPQNLSQEE-FHVQKEIVKEFFIRGPGAACDLTSLYFQE-STMTRCSHQQSPYQLLFGEPYIFEELLSLKIRISPDAFFQINTAGAEMLYRTVGEL-----TGVNSDTILLDICCGTGVIGLSLSQHTSRVLGIELVEQAVEDARWTAAFNGITNSEFHTGRAEKILPGLLKSK--EDGQSIVAVVNPARAGLHYKVIQAIRNFRAIHTLVFVSCKLHGESTRNVIELCCPPDPAKKLLGEPFVLQQVVPVDLFPHTPHCELVLLFTR 486
[Close]
BLAST of tRNA (Uracil-5-)-methyltransferase-like protein vs. UniProt/SwissProt
Match: sp|Q96GJ1|TRM2_HUMAN (tRNA (uracil(54)-C(5))-methyltransferase homolog OS=Homo sapiens OX=9606 GN=TRMT2B PE=1 SV=1)

HSP 1 Score: 143.28 bits (360), Expect = 4.116e-36
Identity = 124/438 (28.31%), Postives = 220/438 (50.23%), Query Frame = 3 
Query:  132 KKILNTTAPLLSLPYDAQLITKQYKIFSAMQNISSLLRKSNTLSPEILEYCSKNKGLLCKIEDIYKSPVIEGYRNKMGAIVGFDEMGNL-TVGHAMTFSNFDKTRKKLVTFSHFCDKLDKKHHILIAKLFEKFIKEDSQFIPNQKKKISLYKGITIRSNRKNETMINIHFRARFTGIDRILGISKELSDQFLTSLQNESINVRSLYCQQMESHFEHTHAQLSCKLIYGDKYLMENVCGKTFYISPLSFFQVNVEVAEMIYQKAKQLVEDYKSKSGKKIXXXXXXXXXXXXXIILSDIVEQVIGVDIVPSAIEXXXXXXXXXXXXXXEHFCKPVEEFLPEFLNSDIAKEYDNVVAVLNPSRDGLSTEFCETLRSLESLKMIVYISCHVEGQTTLNICTLCTVKGDSNPEV--PFSLSKIIPLDPFPHTTHVEVIFQMTR 1436
            +++ +   PL  L Y+ QL  K       +Q + S ++  N +S  +     K++ L C +  I  SPVI GYRNK    V     GN  TVG       +       V  +H  +  +K  H  +A+ +E F+++          +   ++ +T+R+N +  TM  I F  +    +  L + KE+  +F       +  + SLY Q+  +    +H Q   +L++G+ Y+ E +      ISP +FFQ+N   AEM+Y+   +L     +      +LLD+CCGTG +G+ L+    +V+G++++  A+E+A++ A  N + N E      E+ LP  L S   ++  ++VAV+NP+R GL  +  + +R+  ++  +V++SC + G++T N+  LC     +   +  PF L + +P+D FPHT H E++   TR
Sbjct:   82 ERLADVVTPLWRLSYEEQLKVKFAAQKKILQRLESYIQMLNGVS--VTTAVPKSERLSCLLHPIIPSPVINGYRNKSTFSVNRGPDGNPKTVG--FYLGTWRDGNVVCVQSNHLKNIPEK--HSQVAQYYEVFLRQSPLEPCLVFHEGGYWRELTVRTNSQGHTMAIITFHPQKLSQEE-LHVQKEIVKEFFIRGPGAACGLTSLYFQE-STMTRCSHQQSPYQLLFGEPYIFEELLSLKIRISPDAFFQINTAGAEMLYRTVGEL-----TGVNSDTILLDICCGTGVIGLSLAQHTSRVLGIELLEQAVEDARWTAAFNGITNSEFHTGQAEKILPGLLKSK--EDGQSIVAVVNPARAGLHYKVIQAIRNFRAIHTLVFVSCKLHGESTRNVIELCCPPDPAKKLLGEPFVLQQAVPVDLFPHTPHCELVLLFTR 504
[Close]
BLAST of tRNA (Uracil-5-)-methyltransferase-like protein vs. UniProt/SwissProt
Match: sp|Q8BQJ6|TRM2_MOUSE (tRNA (uracil(54)-C(5))-methyltransferase homolog OS=Mus musculus OX=10090 GN=Trmt2b PE=2 SV=1)

HSP 1 Score: 137.502 bits (345), Expect = 4.393e-34
Identity = 127/442 (28.73%), Postives = 209/442 (47.29%), Query Frame = 3 
Query:  132 KKILNTTAPLLSLPYDAQLITKQYKIFSAMQNISSLLRKSNTLSPEILEYCSKNKGLLCKIEDIYKSPVIEGYRNKMGAIVGFDEMGNL-TVGHAMTFSNFDKTRKKLVTFSHFCDKLDK--KHHILIAKLFEKFIKEDSQFIPNQKKKISLYKGITIRSNRKNETMINIHFRARFTGIDRILGISKELSDQFLTSLQNESINVRSLYCQQMESHFEHTHAQLSCKLIYGDKYLMENVCGKTFYISPLSFFQVNVEVAEMIYQKAKQLVEDYKSKSGKKIXXXXXXXXXXXXXIILS--DIVEQVIGVDIVPSAIEXXXXXXXXXXXXXXEHFCKPVEEFLPEFLNSDIAKEYDNVVAVLNPSRDGLSTEFCETLRSLESLKMIVYISCHVEGQTTLNICTLCTVKGDSNPEV--PFSLSKIIPLDPFPHTTHVEVIFQMTR 1436
            +++ +   PL  L Y+AQL  K       +Q++ S L+  + +S  +  +  +++GL C +  I  SP   GYRNK    V     GN  TVG+ +       T K        C+ L    + H  +A+ +E F+++ S        +   ++ + +R+NR+  TM  + F  +    + +      L D F T        + SLY Q+  +    +H Q   +L++G+ ++ E++ G    ISP +FFQ+N   AEM+Y+   +L       SG     L L    GT  I LS      QV G+++V  A+E+A++ A  N V N E      E  LP+ L S   K+    VAV+NP+R GL       +R+  ++  +V++SC   G++T N   LC     +   +  PF L + +P+D FPHT H E++   TR
Sbjct:   71 ERLADVVTPLWRLSYEAQLKVKFEAQKKLLQSLESHLKVLHGVSDTVAAH--QSEGLRCLLHPIIPSPTTTGYRNKSTFSVYRSPDGNPKTVGYYL------GTWKDGNVVCLPCNHLKNIPEKHSQVAQYYEVFLRQSSVEPCLLFHEGGHWRELVVRTNRQGHTMAIVTFHPQGLSEEEVCVQKVTLKD-FFTKGPGAICELTSLYFQE-STMTRCSHQQSPYQLLFGEPHIFEDLLGLKIRISPDAFFQINTAGAEMLYRIIGEL-------SGVNSESLLLDICCGTGVIGLSVAQRASQVHGIELVEQAVEDARWTAAFNGVTNCEFHAGRAETILPQLLKSQ--KDEKLTVAVVNPARAGLHYRVVRAIRNCRTIHTLVFVSCKPHGESTRNFIELCCPPNSAKQLLGDPFVLREAVPVDLFPHTPHCELVLLFTR 493
[Close]
BLAST of tRNA (Uracil-5-)-methyltransferase-like protein vs. UniProt/SwissProt
Match: sp|Q8IZ69|TRM2A_HUMAN (tRNA (uracil-5-)-methyltransferase homolog A OS=Homo sapiens OX=9606 GN=TRMT2A PE=1 SV=2)

HSP 1 Score: 137.887 bits (346), Expect = 1.163e-33
Identity = 120/445 (26.97%), Postives = 218/445 (48.99%), Query Frame = 3 
Query:  135 KILNTTAPLLSLPYDAQLITKQYKIFSAMQNISSLLRKSN-TLSPEILEYCSKNKGLLCKIEDIYKSPVIEGYRNKMGAIVGFD----------EMGNLTVGHAMTFSNFDKTRKKLVTFSHFCDKLDKKHHILIAKLFEKFIKEDSQFIPNQKKKISLYKGITIRSNRKNETMINIHFRARFTGIDRILGISKELSDQFLTSLQNESINVRSLYCQQMESHFEHTHAQLSCKLIYGDKYLMENVCGKTFYISPLSFFQVNVEVAEMIYQKAKQLVEDYKSKSGKKIXXXXXXXXXXXXXIILSDIVEQVIGVDIVPSAIEXXXXXXXXXXXXXXEHFCKPVEEFLPEFLNSDIAKEYDNVVAVLNPSRDGLSTEFCETLRSLESLKMIVYISCHVEGQTTLNICTLCTVKGDSNPEVPFSLSKIIPLDPFPHTTHVEVIFQMTR 1436
            ++ +   PL ++PY  QL  KQ +    +Q ++  +  +N  L P +LE   K+    C +E +  SP    YRNK   +VG             +G    G     + FD          H  +   +     + K F++FI+       + +     +K +T+R++R+++ M   +F  +    + +  +   L+  F T+    +  V  LY  +       +   L  + + GD+ + E++ G TF ISP +FFQVN   AE++Y     +++D+       + +LD+CCGTGT+G+ L+  V++VIGV++ P A+E+A+ NA+ N ++NVE  C   E+ +P  + S +A ++  +VA+L+P R GL ++    +R  ++L+ ++Y+SC+       N   LC    +    +PF   K + +D FP T H E++    R
Sbjct:  167 RVADVVTPLWTVPYAEQLERKQLECEQVLQKLAKEIGSTNRALLPWLLEQRHKHNKACCPLEGVRPSPQQTEYRNKCEFLVGVGVDGEDNTVGCRLGKYKGGTCAVAAPFDTV--------HIPEATKQ-----VVKAFQEFIRSTPYSAYDPETYTGHWKQLTVRTSRRHQAMAIAYFHPQKLSPEELAELKTSLAQHF-TAGPGRASGVTCLYFVEEGQRKTPSQEGLPLEHVAGDRCIHEDLLGLTFRISPHAFFQVNTPAAEVLY----TVIQDWAQLDAGSM-VLDVCCGTGTIGLALARKVKRVIGVELCPEAVEDARVNAQDNELSNVEFHCGRAEDLVPTLV-SRLASQH--LVAILDPPRAGLHSKVILAIRRAKNLRRLLYVSCNPRAAMG-NFVDLCRAPSNRVKGIPFRPVKAVAVDLFPQTPHCEMLILFER 588
[Close]
BLAST of tRNA (Uracil-5-)-methyltransferase-like protein vs. UniProt/SwissProt
Match: sp|A4QP75|TRM2B_DANRE (tRNA (uracil(54)-C(5))-methyltransferase homolog-B OS=Danio rerio OX=7955 GN=trmt2b PE=2 SV=2)

HSP 1 Score: 120.553 bits (301), Expect = 2.913e-28
Identity = 133/457 (29.10%), Postives = 206/457 (45.08%), Query Frame = 3 
Query:  132 KKILNTTAPLLSLPYDAQLITKQYKIFSAMQNISSLLRKSNTLSPEILEYCSKNKGLLCKIEDIYKSPVIEGYRNK---------------MGAIVGFDEMGNLTVGHAMTFSNFDKTRKKLVTFSHFCDKLDKKHHILIAKLFEKFIKEDSQFIPNQKKKISLYKGITIRSNRKNETMINIHFRARFTGIDRILGISKELSDQFLTSLQNESINVRSLYCQQMESHFEHTHAQLSCKLIYGDKYLMENVCGKTFYISPLSFFQVNVEVAEMIYQKAKQLVEDYKSKSGKKIXXXXXXXXXXXXXIILSDIVEQVIGVDIVPSAIEXXXXXXXXXXXXXXEHFCKPVEEFLPEFLNSDIAKEYDNVVAVLNPSRDGLSTEFCETLRSLESLKMIVYISCHVEGQTTLNICTLCT-------VKGDSNPEVPFSLSKIIPLDPFPHTTHVEVIFQMTR 1436
            +++ +T  PL  + Y+ QL   Q+K +   + I  LL+    LS +     S +  L   +  I  SPV +GYRNK               +G  +G  + GN+   HA                 H      K  H ++A+ +E FI+               ++ ITIR+N    TM  ++F  +    +    I K    ++ T        + SLY Q+  +    +H Q   +L+YG  ++ E V G  F ISP SFFQVN E AE +Y+   +L +     +   +     CCGTG +GI LS  +E+VIG++++  A+E+AKFNA  N V N E      E  LP+ + S        + AV+NPSR GL       LR+  +++ +VYISC  +G+   N   LC        + G++     F     +P+D FPHT H E++    R
Sbjct:   64 ERLSDTVTPLWRMTYEDQL---QWK-YEHQKKI--LLKMMKELSQDPTRAFSDH--LNFPLLPIVASPVRDGYRNKSTFSVNKGIDGNPKTLGFYIGTGKAGNIVCVHA----------------DHLLSIPSK--HKMVARCYEDFIRLSPLRPCILFDDGGHWREITIRTNSTGHTMAIVYFHPQSLTPEET-DIHKAALVEYFTQGPGAICQLDSLYFQET-TMTRCSHEQSQYQLLYGQTHIYEEVLGFKFRISPDSFFQVNREAAEALYKTVAELSQPCVGGTLLDV-----CCGTGAIGISLSPQMERVIGIELIEQAVEDAKFNAALNRVCNCEFLAGKAEVVLPDLMGS--LSSDGGLTAVVNPSRAGLHYRVVRALRNHSAIRRLVYISCKPDGEAMRNFRELCCSVGLVRRITGEA-----FKPVVAVPVDLFPHTPHCELVLVFER 480
[Close]
BLAST of tRNA (Uracil-5-)-methyltransferase-like protein vs. TrEMBL
Match: V4A790 (Uncharacterized protein OS=Lottia gigantea OX=225164 GN=LOTGIDRAFT_154241 PE=3 SV=1)

HSP 1 Score: 163.696 bits (413), Expect = 1.825e-41
Identity = 138/439 (31.44%), Postives = 226/439 (51.48%), Query Frame = 3 
Query:  129 MKKILNTTAPLLSLPYDAQLITKQYKIFSAMQNISSLLRKSNTLSPEILEYCSK-NKGLLCKIEDIYKSPVIEGYRNKMGAIVGFDEMGNLTVGHAMTFSNFDKTRKKLVTFSHFCDKLDKKHHILIAKLFEKFIKEDSQFIPNQKKKISLYKGITIRSNRKNETMINIHFRARFTGIDRILGISKELSDQFLTSLQNESINVRSLYCQQMESHFEHTHAQLSCKLIYGDKYLMENVCGKTFYISPLSFFQVNVEVAEMIYQKAKQLVEDYKSKSGKKIXXXXXXXXXXXXXIILSDIVEQVIGVDIVPSAIEXXXXXXXXXXXXXXEHFCKPVEEFLPEFL--NSDIAKEYDNVVAVLNPSRDGLSTEFCETLRSLESLKMIVYISCHVEGQTTLNICTLCTVKGDSNPEVPFSLSKIIPLDPFPHTTHVEVIFQMTR 1436
            + ++  T  PL  +PY+ QL+ K+  +   ++ I ++++ +   S E L+  ++ N G +C+ +++  SPV EGYRNK   ++GFD   N+  G+ +   N D+   K        D     HH  I +L E+FIK     +P ++    L++  ++R+    ETM+ +         +  L ++K+   ++ T       N+ SLY +   S     HA      + G KY+ E +  K F I+P SFFQVN   AE++Y     L+    + +     LLD+CCGTGT+GI+LS+ V+QV G+++   AIE AK NA  N+V N++  C   E+ L + +  +S + K  DN+V VLNP R G  ++F   LR + ++   ++++C   G    N+  L       N   PFS    + +D FPHT H E+I  MTR
Sbjct:    8 VNRLARTVTPLHKVPYNEQLVKKEKVMRRVLKKIYNIIKHNRKSSTEFLDNQAELNSGQICEFQEVKPSPVTEGYRNKHAILIGFDVNKNIIAGYPLQKQNDDQIHVKADKLCIISD-----HHKYINQLLEEFIKRPP--LPAEEYN-GLWRSGSVRTTTTGETMLTLTVNPYLLSQED-LEMAKKSYIEYFTDGNGAQANIHSLYMELCSSRIPR-HANSQLLHLSGQKYITEKLNDKEFRITPYSFFQVNTHCAEVLYNNISDLI----NINSGTTHLLDMCCGTGTIGILLSNYVDQVCGIEVSKEAIECAKENALLNDVKNIKFVCGTAEDVLNQLILEDSPVLKA-DNLVVVLNPGRLGFKSKFIRRLRRISAINHFIFVTCKPSGVIIQNLVQLMMDDTKGNLGEPFSPQTSVAVDLFPHTKHCELIISMTR 431
[Close]
BLAST of tRNA (Uracil-5-)-methyltransferase-like protein vs. TrEMBL
Match: A0A087RFN0 (tRNA (Uracil(54)-C(5))-methyltransferase (Fragment) OS=Aptenodytes forsteri OX=9233 GN=AS27_15353 PE=3 SV=1)

HSP 1 Score: 163.696 bits (413), Expect = 2.880e-41
Identity = 139/438 (31.74%), Postives = 222/438 (50.68%), Query Frame = 3 
Query:  132 KKILNTTAPLLSLPYDAQLITKQYKIFSAMQNISSLLRKSNTLSPEILEYCSKNKGLLCKIEDIYKSPVIEGYRNKMGAIVGFDEMGNL-TVGHAMTFSNFDKTRKKLVTFSHFCDKLDKKHHILIAKLFEKFIKEDSQFIPNQKKKISLYKGITIRSNRKNETMINIHFRARFTGIDRILGISKELSDQFLTSLQNESINVRSLYCQQMESHFEHTHAQLSCKLIYGDKYLMENVCGKTFYISPLSFFQVNVEVAEMIYQKAKQLVEDYKSKSGKKIXXXXXXXXXXXXXIILSDIVEQVIGVDIVPSAIEXXXXXXXXXXXXXXEHFCKPVEEFLPEFLNSDIAKEYDNVVAVLNPSRDGLSTEFCETLRSLESLKMIVYISCHVEGQTTLNICTLCTV--KGDSNPEVPFSLSKIIPLDPFPHTTHVEVIFQMTR 1436
            +++ N   PL  LPY  QL  K       +Q ++S L        E+     K  GL C ++ +  SPVI GYRNK    V     GNL TVG    +    + R  +   ++  + +  KH   +A+ +E+FI+           K   ++ + +R+     TM  + F  +  G    L   K L  +F          + SLY Q+  +    +H Q   +L++G+ ++ E V G  F ISP +FFQVN   AE++YQ   +L     S++G   +LLD+CCGTGT+G+ L+  + ++IG+++V  AIE+A++NA  N ++N E      E  LP+ L+S   ++   +VAV+NPSR GL     + +R+ +S++ ++YISC  EG+   N+  LC     G      PF+ +  IP D FPHT H E++  +TR
Sbjct:   53 ERLANAVTPLWRLPYQEQLQVKYESQRKILQTLASRLE-------ELGIDAQKPGGLCCPLQPVVPSPVINGYRNKSTFSVNRGPDGNLKTVG---LYVGTGRARNIVCVKANHMENIPPKHK-QVAQCYEEFIRHSPLDPCILFHKGGHWRELVVRTTSCGHTMAIVTFHPQELG-QEALATQKALLKEFFVCGPGTVCALTSLYFQE-STMTRCSHEQSPFQLLHGEPHIFEEVLGLKFRISPDAFFQVNTGGAEVLYQAVGEL-----SQAGGDTVLLDICCGTGTIGLSLAHQMSKIIGIEVVEKAIEDARWNAAFNGISNCEFHSGKAEAMLPQLLSS--WEDAQPLVAVVNPSRAGLHYRVVQAIRNCKSIRRLLYISCKPEGEAMRNLLELCCPPDPGKKLAGEPFAPTLAIPFDMFPHTVHCELVLLLTR 470
[Close]
BLAST of tRNA (Uracil-5-)-methyltransferase-like protein vs. TrEMBL
Match: A0A1V4KYH3 (tRNA (Uracil(54)-C(5))-methyltransferase-like protein OS=Patagioenas fasciata monilis OX=372326 GN=TRMT2B PE=3 SV=1)

HSP 1 Score: 162.925 bits (411), Expect = 8.246e-41
Identity = 140/442 (31.67%), Postives = 228/442 (51.58%), Query Frame = 3 
Query:  132 KKILNTTAPLLSLPYDAQLITKQYKIFSAMQNISSLLRKSNTLSPEILEYCSKNKGLLCKIEDIYKSPVIEGYRNKMGAIVGFDEMGNL-TVGHAMTFSNFDKTRKKLVTFSHFCDKLDKKHHILIAKLFEKFIK---EDSQFIPNQKKKISLYKGITIRSNRKNETMINIHFRARFTGIDRILGISKELSDQFLTSLQNESINVRSLYCQQMESHFEHTHAQLSCKLIYGDKYLMENVCGKTFYISPLSFFQVNVEVAEMIYQKAKQLVEDYKSKSGKKIXXXXXXXXXXXXXIILSDIVEQVIGVDIVPSAIEXXXXXXXXXXXXXXEHFCKPVEEFLPEFLNSDIAKEYDNVVAVLNPSRDGLSTEFCETLRSLESLKMIVYISCHVEGQTTLNICTLCTVKGDSNPEV---PFSLSKIIPLDPFPHTTHVEVIFQMTR 1436
            +++ N   PL  LPY  QL  K       +Q ++S L+       E+     +  GL C ++ +  SPVI GYRNK    V     GN  TVG    +    + R  +   ++  + +  KH   +A+ +E FI+    DS  + ++      ++ + +R+  +  TM  + F  +  G    L + K L  +F T        + SLY Q+  +    +H Q   +L++G+ ++ E V G  F ISP +FFQVN + AE++YQ   +L++     +G   +LLD+CCGTGT+G+ L+  V +VIG+++V  AIE+A++NA  N ++N E      E  LP+ L+S   +    +VAV+NPSR GL       +R+ +S++ ++YISC  EG+   N   LC    D   ++   PF+ + +IP D FPHT H E++   TR
Sbjct:   59 ERLANAVTPLWRLPYQEQLQVKYESQRKILQTLASRLK-------ELGIDVQQPDGLCCPLQPVVPSPVITGYRNKSTFSVNRGPDGNPKTVG---LYVGTGRARNIVCVKANHMENIPSKHK-QVAQCYEDFIRCSPLDSCLLFHEGGH---WRELVVRTTSRGHTMAIVTFHPQDLG-QEALAMQKALLKEFFTCGPGRVCALTSLYFQE-STMTRCSHEQSPFQLLHGEPHIFEEVLGLKFRISPDAFFQVNTDGAEVLYQAVGELIQ-----AGGDTVLLDICCGTGTIGLSLAHQVSKVIGIEVVAKAIEDARWNAAFNGISNCEFHGGRAEAVLPQLLSS--WESPQPLVAVVNPSRAGLHYRVVRAIRNCKSIRRLLYISCKPEGEAMRNFLELCCPP-DPRKKLAGEPFTPTLVIPFDMFPHTVHCELVLLFTR 476
[Close]
BLAST of tRNA (Uracil-5-)-methyltransferase-like protein vs. TrEMBL
Match: A0A091FSJ1 (tRNA (Uracil(54)-C(5))-methyltransferase (Fragment) OS=Cuculus canorus OX=55661 GN=N303_15145 PE=3 SV=1)

HSP 1 Score: 160.999 bits (406), Expect = 2.991e-40
Identity = 138/438 (31.51%), Postives = 221/438 (50.46%), Query Frame = 3 
Query:  132 KKILNTTAPLLSLPYDAQLITKQYKIFSAMQNISSLLRKSNTLSPEILEYCSKNKGLLCKIEDIYKSPVIEGYRNKMGAIVGFDEMGNL-TVGHAMTFSNFDKTRKKLVTFSHFCDKLDKKHHILIAKLFEKFIKEDSQFIPNQKKKISLYKGITIRSNRKNETMINIHFRARFTGIDRILGISKELSDQFLTSLQNESINVRSLYCQQMESHFEHTHAQLSCKLIYGDKYLMENVCGKTFYISPLSFFQVNVEVAEMIYQKAKQLVEDYKSKSGKKIXXXXXXXXXXXXXIILSDIVEQVIGVDIVPSAIEXXXXXXXXXXXXXXEHFCKPVEEFLPEFLNSDIAKEYDNVVAVLNPSRDGLSTEFCETLRSLESLKMIVYISCHVEGQTTLNICTLCTV--KGDSNPEVPFSLSKIIPLDPFPHTTHVEVIFQMTR 1436
            +++ N   PL  LPY  QL  K       +Q ++S L        E+     K  GL C ++ +  SPVI GYRNK    V     GN  TVG    +    + R  +   ++  + +  KH   +A+ +E++I+           +   ++ + +R+  +  TM  I F  +  G    L   K L  +F T        + SLY Q+  +    +H Q   +L++G+ ++ E V G  F ISP +FFQVN   AE++YQ  ++L     S++G   +LLD+CCGTGT+G+ L+  V ++IG+++V  A+E+A++NA  N ++N E      E  LP+ L+S   ++   +VAV+NPSR GL       +R+ +S++ ++YISC  EG+   N   LC     G      PF+ +  IP D FPHT H E++   TR
Sbjct:   47 ERLANAVTPLWRLPYQEQLQVKYESQRKILQTLTSRLE-------ELGIDAQKPDGLCCPLQPVVPSPVINGYRNKSTFSVNQGPDGNPKTVG---LYVGTGRARNIVCVKANHVENIPPKHK-EVAQWYEEYIRCSPLEPCLLFHEGGHWRELVVRTTSRGHTMAIITFHPQELG-QEALATQKALLKEFFTCGPGAVCALTSLYFQE-STMTRCSHEQSPFQLLHGEPHIFEEVLGLKFRISPDAFFQVNTGGAEVLYQAVREL-----SQAGGDTVLLDICCGTGTIGLSLAHQVSKIIGIEVVEKAVEDARWNAAFNGISNCEFHSGKAEAVLPQLLSS--WEDSQALVAVVNPSRAGLHYRVVRAIRNCKSIRRLLYISCKPEGEAMRNFLELCCPPNPGKKLAGEPFAPTLAIPFDMFPHTLHCELVLLFTR 464
[Close]
BLAST of tRNA (Uracil-5-)-methyltransferase-like protein vs. TrEMBL
Match: A0A093HQC0 (tRNA (Uracil(54)-C(5))-methyltransferase (Fragment) OS=Tyto alba OX=56313 GN=N341_09548 PE=3 SV=1)

HSP 1 Score: 160.614 bits (405), Expect = 4.723e-40
Identity = 138/438 (31.51%), Postives = 222/438 (50.68%), Query Frame = 3 
Query:  132 KKILNTTAPLLSLPYDAQLITKQYKIFSAMQNISSLLRKSNTLSPEILEYCSKNKGLLCKIEDIYKSPVIEGYRNKMGAIVGFDEMGNL-TVGHAMTFSNFDKTRKKLVTFSHFCDKLDKKHHILIAKLFEKFIKEDSQFIPNQKKKISLYKGITIRSNRKNETMINIHFRARFTGIDRILGISKELSDQFLTSLQNESINVRSLYCQQMESHFEHTHAQLSCKLIYGDKYLMENVCGKTFYISPLSFFQVNVEVAEMIYQKAKQLVEDYKSKSGKKIXXXXXXXXXXXXXIILSDIVEQVIGVDIVPSAIEXXXXXXXXXXXXXXEHFCKPVEEFLPEFLNSDIAKEYDNVVAVLNPSRDGLSTEFCETLRSLESLKMIVYISCHVEGQTTLNICTLCTV--KGDSNPEVPFSLSKIIPLDPFPHTTHVEVIFQMTR 1436
            +++ N   PL  LPY  QL  K       +Q ++S L        E+     K  GL C +  +  SP+I GYRNK    V     GN  TVG    +    K R  +   ++  + +  KH   +++ +E+FI+           +   ++ + +R+     TM  I F  +  G    L   K L  +F T        + SLY Q+  +    TH Q   +L++G+ +++E V G  F ISP +FFQ+N   AE++YQ  ++L     S++G+  +LLD+CCGTGT+G+ L+  V +VIG+++V  AIE+A++NA  N ++N E      E  LP+ L+  + ++   +VAV+NPSR GL       +R+ +S++ ++YISC  EG+   N   LC     G +    PF+    +P D FPHT H E++  +TR
Sbjct:   47 ERLANVVTPLWRLPYQKQLQVKYESQRKILQTLASRLE-------ELGIDVKKPGGLCCPLRPVVPSPIINGYRNKSTFSVNRGPDGNPKTVG---MYVGTGKARNIVCVKANHMENIPPKHK-QVSQCYEEFIRRSPLDPCILFHEGGHWRELVVRTTSCGHTMAIITFHPQDLG-QEALATQKALLKEFFTCGPGTVCALTSLYFQE-STMTRCTHEQSPFQLLHGEPHILEEVLGLKFRISPDAFFQINTGGAEVLYQAVREL-----SQAGEDTILLDICCGTGTIGLALAHQVSKVIGIEVVEKAIEDARWNAAFNGISNCEFHSGKAEAMLPQLLS--LWEDDRPLVAVVNPSRAGLHYRAVRAIRNCKSIRRLLYISCKPEGEAMRNFLELCCPPDPGKNLAGDPFAPMLAVPFDMFPHTVHCELVLLLTR 464
[Close]
BLAST of tRNA (Uracil-5-)-methyltransferase-like protein vs. Ensembl Cavefish
Match: trmt2a (tRNA methyltransferase 2 homolog A [Source:NCBI gene;Acc:103033829])

HSP 1 Score: 151.754 bits (382), Expect = 2.701e-39
Identity = 129/439 (29.38%), Postives = 219/439 (49.89%), Query Frame = 3 
Query:  132 KKILNTTAPLLSLPYDAQLITKQYKIFSAMQNISSLLRKSN-TLSPEILEYCSKNKGLLCKIEDIYKSPVIEGYRNKMGAIVGFDEMG-NLTVGHAMTFSNFDKTRKKLVTFSHFCDKL-DKKHHILIAKLFEKFIKEDSQFIPNQKKKISLYKGITIRSNRKNETMINIHFRARFTGIDRILGISKELSDQFLTSLQNESINVRSLYCQQMESHFEHTHAQLSCKLIYGDKYLMENVCGKTFYISPLSFFQVNVEVAEMIYQKAKQLVE-DYKSKSGKKIXXXXXXXXXXXXXIILSDIVEQVIGVDIVPSAIEXXXXXXXXXXXXXXEHFCKPVEEFLPEFLNSDIAKEYDNVVAVLNPSRDGLSTEFCETLRSLESLKMIVYISCHVEGQTTLNICTLCTVKGDSNPEVPFSLSKIIPLDPFPHTTHVEVIFQMTR 1436
            ++I     PL  +PY+ QL  K+ ++ + +QN++  +  +N  + P +     KNK + C +E I  SP    YRNK   ++G    G + TVG  +      K +          D +   K  I + + F+++I+     + + +     ++ +T+R++R N+ M  + F  +    + +  + K L  Q+ T        + SL+  +M          L C+L+ GD+++ E + G  F ISP SFFQ N   AE++Y    +  + D +S       +LD+CCGTGT+GI L+  V++VIG+++   A+E+AK NAKANN+ NVE  C   E+  P  LN+ ++    NV A+++P R GL ++    +R  E LK +VY++C+ +     N   LC    +     PF   + + +D FP T H E I    R
Sbjct:  199 EQIARAVTPLWKIPYEEQLRRKEQEVETVLQNLAREIGNNNKAMLPWLFVQKEKNKNMCCPLEPIRPSPQQTEYRNKCEFVIGMGADGEDKTVGFRL-----GKYKGGSCAVVSPADTVHTPKSAIRVVQAFQRYIRTTPYAVYSPETYEGHWRQLTVRTSRTNQIMAMVFFNPQKLSEEELGSLKKSLG-QYFTEGDGRESRITSLHFVRMGQRTSAGTEDLPCELVAGDEWIHEELLGLKFRISPHSFFQTNTPAAEVLYSAVGEWAQLDQES------TVLDVCCGTGTIGISLAKRVKKVIGIELCQEAVEDAKVNAKANNLTNVEFHCGKAEDIFPTVLNAVVSP---NVTAIVDPPRAGLHSKVILAIRRAEHLKRLVYVACNAKAAKN-NFVDLCRAPSNRVRGSPFRPVRAMAVDLFPQTMHCETILLFER 621
[Close]
BLAST of tRNA (Uracil-5-)-methyltransferase-like protein vs. Ensembl Nematostella
Match: EDO47401 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RLV8])

HSP 1 Score: 125.176 bits (313), Expect = 1.513e-30
Identity = 120/442 (27.15%), Postives = 213/442 (48.19%), Query Frame = 3 
Query:  135 KILNTTAPLLSLPYDAQLITKQYKIFSAMQNISSLLRKSNTLSPEILEYCSK-NKGLLCKIEDIYKSPVIEGYRNKMGAIVGFDEMGNLTVGHAMTFSNFDKTRKKLVTFSHFC--DKLDKKHHILIAKLFEKFIKEDSQFIPNQKKKISLYKG----ITIRSNRKNETMINIHFRARFTGIDRILGISKELSDQFLTSLQNESINVRSLYCQQMESHFEHTHAQLSCKLIYGDKYLMENVCGKTFYISPLSFFQVNVEVAE-MIYQKAKQLVEDYKSKSGKKIXXXXXXXXXXXXXIILSDIVEQVIGVDIVPSAIEXXXXXXXXXXXXXXEHFCKPVEEFLPEFLNSDIAKEYDNVVAVLNPSRDGLSTEFCETLRSLESLKMIVYISCHVEGQTTLNICTLCTVKGDSNPEVPFSLSKIIPLDPFPHTTHVEVIFQMTR 1436
            ++ ++  PL S PY  QL  K  KI   ++ ++  ++    L    +E  SK   G+ C+++   +SP+++GYRNK    +     G  TVG      ++ +    ++   + C   K  KK    +A  F+K+I+      P  +  +  + G    +T+RS    E+M  + +  +      I   + ++   FL   + +  N+ SLY Q   +      +      + G+K + E +C   F ISP +FFQVN + AE MI+  ++ + E       K+  LLD+CCGTG++G+ L+  V +VIGV+++ SA+E+AK NA  N + N E+ C   E+    + +    + +   + +++P R G+     + +R  + L  ++Y+SC  +     N+  LC         VPF L   +P+D FPHT H E++    R
Sbjct:  274 RLNDSVTPLWSKPYSEQLEFKNQKIEEFLKKLTKQIKDMMELKGTWVEEESKVRNGICCELQKCEESPIVDGYRNKCEFTIAEGPKGAKTVG--FRVGSYKEGYSGVIEPFNCCHISKTAKK----VASAFQKYIRNS----PLPEYDLCFHTGHWQTLTVRSTVLGESMAIVQYNPQKMSATEIATENDKIRRYFLEG-EGKECNLTSLYAQVNATRAVGCQSPAPFTHLLGEKVIHEVICDVKFGISPNAFFQVNSKAAEKMIHIISRWMGEQ------KETTLLDICCGTGSIGLCLAKYVSKVIGVEVIQSAVEDAKQNAIMNGITNAEYVCGKAEDVF-TYKSMKRLRRFTQCIGIMDPPRAGVHKSVIDAIRKYDKLDKLIYVSCAPD-HAIPNLLDLCRPTSKRVKSVPFRLVSAVPVDLFPHTKHCELLMLFQR 696
[Close]
BLAST of tRNA (Uracil-5-)-methyltransferase-like protein vs. Ensembl Medaka
Match: trmt2b (tRNA methyltransferase 2 homolog B [Source:NCBI gene;Acc:101156035])

HSP 1 Score: 130.183 bits (326), Expect = 1.642e-32
Identity = 132/449 (29.40%), Postives = 207/449 (46.10%), Query Frame = 3 
Query:  114 NIVFEMKKILNTTAPLLSLPYDAQLITKQ-YKIFSAMQNISSLLRKSNTLSPEILEYCSKNKGLLCKIEDIYKSPVIEGYRNKMGAIVGFDEMGNL-TVGHAMTFSNFDKTRKKLVTFSHFCDKLDKKHHILIAKLFEKFIKEDSQFIPNQKKKISLYKGITIRSNRKNETMINIHFRARFTGIDRILGISKELSDQFLTSLQNESINVRSLYCQQMESHFEHTHAQLSCKLIYGDKYLMENVCGKTFYISPLSFFQVNVEVAEMIYQKAKQLVEDYKSKSGKKIXXXXXXX----XXXXXXIILSDIVEQVIGVDIVPSAIEXXXXXXXXXXXXXXEHFCKPVEEFLPEFLNSDIAKEYDNVVAVLNPSRDGLSTEFCETLRSLESLKMIVYISCHVEGQTTLNICTLCTVKGDSNPEVPFSLSKI--IPLDPFPHTTHVEVIFQMTR 1436
            N+ +E +++ +   PL  L YD QL  KQ ++  +  Q +  L     + SP       KNK L   +  I  SPV +GYRNK    V     GN  TVG         +     V   H  +  +K  H L+A+ ++ FI++              ++ +T+R+N    TM  ++F  +    + +     +L D F+         + SL+ Q+  S    TH +   +L+YG  ++ E V G  F ISP +FFQVN   AE++Y   + L+     K  K+    D       GTG +GI  S  VE+VIG++++  A+E+A  NA  NNV N +      E  LP  +  D++     + AV+NP+R GL       LR+  +++ +VY+SC  +G+   N   LC            + S I  IP+D FPHT H E++    R
Sbjct:   49 NLSWE-ERLADAVTPLWRLSYDEQLELKQKHQETTLSQLVGHLSGDPKSSSP------VKNK-LTFPLLPILPSPVRDGYRNKSTFSVNRGADGNTKTVG--FYLGTGKQGNIVCVNGDHLLNMPEK--HKLVARCYQDFIRQSPLDPCLLFHSGGHWREVTVRTNATGHTMAIVYFHPQTLTPEEVAVHQTDLVDYFMRG-PGSVCQLDSLFFQE-SSMTRCTHEKSPYQLLYGQPHIYETVLGFKFRISPDAFFQVNQGAAEVLYGTLRDLISANTEKGEKQGKPTDTLLDVCCGTGAIGITTSPRVEKVIGIELIEQAVEDAVHNAALNNVLNCKFISGKAEAVLPGLI-VDLSCTGGGLTAVVNPARAGLHYRVVRALRNQPNIRRLVYVSCKPDGEAMRNFRELCCPPDPQKKLTGEAFSPILAIPVDMFPHTPHCELVLLFER 482
[Close]
BLAST of tRNA (Uracil-5-)-methyltransferase-like protein vs. Ensembl Medaka
Match: trmt2a (tRNA methyltransferase 2 homolog A [Source:NCBI gene;Acc:101155696])

HSP 1 Score: 129.028 bits (323), Expect = 1.215e-31
Identity = 125/441 (28.34%), Postives = 211/441 (47.85%), Query Frame = 3 
Query:  135 KILNTTAPLLSLPYDAQLITKQYKIFSAMQNISSLLRKSN-TLSPEILEYCSKNKGLLCKIEDIYKSPVIEGYRNKMGAIVGFDEMG-NLTVGHAMTFSNFDKTRKKLVTFSHFCDKLDKKHHILIA-----KLFEKFIKEDSQFIPNQKKKISLYKGITIRSNRKNETMINIHFRARFTGIDRILGISKELSDQFLTSLQNESINVRSLYCQQMESHFEHTHAQLSCKLIYGDKYLMENVCGKTFYISPLSFFQVNVEVAEMIYQKAKQLVEDYKSKSGKKIXXXXXXXXXXXXXIILSDIVEQVIGVDIVPSAIEXXXXXXXXXXXXXXEHFCKPVEEFLPEFLNSDIAKEYDNVVAVLNPSRDGLSTEFCETLRSLESLKMIVYISCHVEGQTTLNICTLCTVKGDSNPEVPFSLSKIIPLDPFPHTTHVEVIFQMTR 1436
            +I N   PL +  Y+ QL  K+ ++   +Q ++  +  +N  + P +     K+  + C +E I  SP    YRNK   ++     G + T+G       +      +V  S  C       H+  A     + F+KFI+       + +     +K +T+R+ R  E M  + F  +    + +  +   +   F T    +   V SLY  +           L C+L+ G+  + E + G  F ISP SFFQVN   AE++Y      V D+ ++  +   +LD+CCGTGT+GI L+  V++VIG+++   A+E+AK NAK N ++NVE  C   E+  P+ LN+ ++    ++ AV++P R GL  +    +R  E LK +VY++C+ +   T N   LC    +     PF   + + +D FP T HVE++    R
Sbjct:  186 QIANVVTPLWNSTYEEQLKMKEQEVVGVLQKLAKEIGSTNKAMLPWLFAQKVKHNKMCCPLEAIRPSPTQTEYRNKCEFLISVGADGEDKTIG--FRLGKYKGGSCAVVGPSATC-------HVSAAAKTVVREFQKFIRTTPYSAYSPETYEGHWKQLTVRTTRAKEAMAVVFFNPQKLEEEDLNALKTSMR-TFFTEGDGKDSGVTSLYFVRDGQRKSPNPEDLPCELVAGESCIHEELLGLRFRISPHSFFQVNTAAAEVLY----STVGDW-AQLDQDSTVLDVCCGTGTIGISLAKRVKKVIGIELCQEAVEDAKVNAKLNGLSNVEFHCGKAEDVFPDILNALVSP---SLTAVVDPPRAGLHPKVILAIRRAEHLKRLVYVACNAKAAMT-NFIDLCRAPSNRVHGSPFRPVRAMAVDLFPQTIHVEMLLLFER 607
[Close]
BLAST of tRNA (Uracil-5-)-methyltransferase-like protein vs. Ensembl Medaka
Match: trmt2a (tRNA methyltransferase 2 homolog A [Source:NCBI gene;Acc:101155696])

HSP 1 Score: 100.138 bits (248), Expect = 4.674e-22
Identity = 114/416 (27.40%), Postives = 194/416 (46.63%), Query Frame = 3 
Query:  135 KILNTTAPLLSLPYDAQLITKQYKIFSAMQNISSLLRKSN-TLSPEILEYCSKNKGLLCKIEDIYKSPVIEGYRNKMGAIVGFDEMG-NLTVGHAMTFSNFDKTRKKLVTFSHFCDKLDKKHHILIA-----KLFEKFIKEDSQFIPNQKKKISLYKGITIRSNRKNETMINIHFRARFTGIDRILGISKELSDQFLTSLQNESINVRSLYCQQMESHFEHTHAQLSCKLIYGDKYLMENVCGKTFYISPLSFFQVNVEVAEMIYQKAKQLVEDYKSKSGKKIXXXXXXXXXXXXXIILSDIVEQVIGVDIVPSAIEXXXXXXXXXXXXXXEHFCKPVEEFLPEFLNSDIAKEYDNVVAVLNPSRDGLSTEFCETLRSLESLKMIVYISCHVEGQTTLNI----CTLCTVKGDSNP 1349
            +I N   PL +  Y+ QL  K+ ++   +Q ++  +  +N  + P +     K+  + C +E I  SP    YRNK   ++     G + T+G       +      +V  S  C       H+  A     + F+KFI+       + +     +K +T+R+ R  E M  + F  +    + +  +   +   F T    +   V SLY  +           L C+L+ G+  + E + G  F ISP SFFQVN   AE++Y      V D+ ++  +   +LD+CCGTGT+GI L+  V++VIG+++   A+E+AK NAK N ++NVE  C   E+  P+ LN+ ++    ++ AV++P R GL    C  LR   S+  +  +S       +L      C  C+ +  +NP
Sbjct:  186 QIANVVTPLWNSTYEEQLKMKEQEVVGVLQKLAKEIGSTNKAMLPWLFAQKVKHNKMCCPLEAIRPSPTQTEYRNKCEFLISVGADGEDKTIG--FRLGKYKGGSCAVVGPSATC-------HVSAAAKTVVREFQKFIRTTPYSAYSPETYEGHWKQLTVRTTRAKEAMAVVFFNPQKLEEEDLNALKTSMR-TFFTEGDGKDSGVTSLYFVRDGQRKSPNPEDLPCELVAGESCIHEELLGLRFRISPHSFFQVNTAAAEVLY----STVGDW-AQLDQDSTVLDVCCGTGTIGISLAKRVKKVIGIELCQEAVEDAKVNAKLNGLSNVEFHCGKAEDVFPDILNALVSP---SLTAVVDPPRAGL---LCAELRPTGSMDPLSVLSEPWRWICSLRRYMWKCCFCSREWTTNP 580
[Close]
BLAST of tRNA (Uracil-5-)-methyltransferase-like protein vs. Planmine SMEST
Match: SMESG000077010.1 (SMESG000077010.1)

HSP 1 Score: 826.624 bits (2134), Expect = 0.000e+0
Identity = 435/436 (99.77%), Postives = 435/436 (99.77%), Query Frame = 3 
Query:  129 MKKILNTTAPLLSLPYDAQLITKQYKIFSAMQNISSLLRKSNTLSPEILEYCSKNKGLLCKIEDIYKSPVIEGYRNKMGAIVGFDEMGNLTVGHAMTFSNFDKTRKKLVTFSHFCDKLDKKHHILIAKLFEKFIKEDSQFIPNQKKKISLYKGITIRSNRKNETMINIHFRARFTGIDRILGISKELSDQFLTSLQNESINVRSLYCQQMESHFEHTHAQLSCKLIYGDKYLMENVCGKTFYISPLSFFQVNVEVAEMIYQKAKQLVEDYKSKSGKKIXXXXXXXXXXXXXIILSDIVEQVIGVDIVPSAIEXXXXXXXXXXXXXXEHFCKPVEEFLPEFLNSDIAKEYDNVVAVLNPSRDGLSTEFCETLRSLESLKMIVYISCHVEGQTTLNICTLCTVKGDSNPEVPFSLSKIIPLDPFPHTTHVEVIFQMTR 1436
            MKKILNTTAPLLSLPYDAQLITKQYKIFSAMQNISSLLRKSNTLSPEILEYCSKNKGLLCKIEDIYKSPVIEGYRNKMGAIVGFDEMGNLTVGHAMTFSNFDKTRKKLVTFSHFCDKLDKKHHILIAKLFEKFIKEDSQFIPNQKKKISLYKGITIRSNRKNETMINIHFRARFTGIDRILGISKELSDQFLTSLQNESINVRSLYCQQMESHFEHTHAQLSCKLIYGDKYLMENVCGKTFYISPLSFFQVNVEVAEMIYQKAKQLVEDYKSKSGKKILLLDLCCGTGTLGIILSDIVEQVIGVDIVPSAIENAKFNAKANNVNNVEHFCKPVEEFLPEFLNSDIAKEYDNVVAVLNPSRDGLSTEFCETLRS ESLKMIVYISCHVEGQTTLNICTLCTVKGDSNPEVPFSLSKIIPLDPFPHTTHVEVIFQMTR
Sbjct:    1 MKKILNTTAPLLSLPYDAQLITKQYKIFSAMQNISSLLRKSNTLSPEILEYCSKNKGLLCKIEDIYKSPVIEGYRNKMGAIVGFDEMGNLTVGHAMTFSNFDKTRKKLVTFSHFCDKLDKKHHILIAKLFEKFIKEDSQFIPNQKKKISLYKGITIRSNRKNETMINIHFRARFTGIDRILGISKELSDQFLTSLQNESINVRSLYCQQMESHFEHTHAQLSCKLIYGDKYLMENVCGKTFYISPLSFFQVNVEVAEMIYQKAKQLVEDYKSKSGKKILLLDLCCGTGTLGIILSDIVEQVIGVDIVPSAIENAKFNAKANNVNNVEHFCKPVEEFLPEFLNSDIAKEYDNVVAVLNPSRDGLSTEFCETLRSFESLKMIVYISCHVEGQTTLNICTLCTVKGDSNPEVPFSLSKIIPLDPFPHTTHVEVIFQMTR 436
[Close]
BLAST of tRNA (Uracil-5-)-methyltransferase-like protein vs. Planmine SMEST
Match: SMESG000008614.1 (SMESG000008614.1)

HSP 1 Score: 125.176 bits (313), Expect = 1.701e-30
Identity = 129/448 (28.79%), Postives = 212/448 (47.32%), Query Frame = 3 
Query:  171 PYDAQLITKQYKIFSAMQNISSLLRKSNTLSPEILEYCSK--NKGLLCKIEDIYKSPVIEGYRNKMGAIVGFD-EMGNLTVG--HAMTFSNFDK---TRKKLVTFSHFCDKLDKKHHILIAKLFEKFIKEDSQFIPNQKKKISLYKGITIRSNRKN-------------ETMINIHFRARFTGIDRILGISKELSDQFLTSLQNESINVRSLYCQQMESHFEHTHAQLSCKLIYGDKYLMENVCGKTFYISPLSFFQVNVEVAEMIYQKAKQLVEDY--KSKSGKKIXXXXXXXXXXXXXIILSDIVEQVIGVDIVPSAIEXXXXXXXXXXXXXXEHFCKPVEEFLPEFLNSDIAKEYDN---VVAVLNPSRDGLSTEFCETLRSLESLKMIVYISCHVEGQTTLNICTLCTVKGDSNPEVPFSLSKIIPLDPFPHTTHVEVIFQMTR 1436
            PYD QLI K+ KI   +QNI   + K N L  E   + S+  +  ++C +  I  SPV + YRNK    +GF  E      G  H      FD    T    +        L   + IL   +  +F    S F P   +     +GIT+R+   N               ++ I    +    + +  I    ++ FLT  + ++ NV SL   + +++ +      + K I+G  ++ E +    F IS  +FFQVN   AE +Y   +QL+ D+   S SGK + LLD+CCGTGT+G++++D+V++VIGV++ P ++E+A +N + NN+          E+ +   L++    + +N    VA+++P R GL     + LR  E +K ++++SC+ E     N               PF     +P+D FPHT HVE++  +TR
Sbjct:  141 PYDNQLIAKENKIKGILQNIFLKINKENILIKESKNFYSQINDNNVVCPLLPIIPSPVTDCYRNKCEFTIGFTLENTGPIAGFCHGKYRGGFDAIGDTSCLPIIPMQMSKVLHHINDILPQLISGEFTPNLSVFKPGTDQ--GHLRGITLRTGSINLDKSPTDSSEDIQSLLMIIDIHPQLLSENELQSIGDFFAEYFLTG-KGKTCNVTSLLLTRRKNNSDPLD-NTTTKTIFGSGFVYEKLMNLNFRISYNAFFQVNTLAAETLYSTIRQLLIDHVIDSSSGKHVTLLDVCCGTGTIGLVMADLVDKVIGVEMCPESVEDANYNKELNNITKATFHAGKAEDMISNLLSTAEKSQENNYREFVAIVDPPRAGLHISVIQALRRCELIKKVIFVSCNPEAAYN-NFIDFARPISKRTHGCPFIPEIAVPVDLFPHTPHVELVVLLTR 583
The following BLAST results are available for this feature:
BLAST of tRNA (Uracil-5-)-methyltransferase-like protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
TRMT2B8.570e-3728.31tRNA methyltransferase 2 homolog B [Source:HGNC Sy... [more]
TRMT2B8.570e-3728.31tRNA methyltransferase 2 homolog B [Source:HGNC Sy... [more]
TRMT2B8.570e-3728.31tRNA methyltransferase 2 homolog B [Source:HGNC Sy... [more]
TRMT2A2.421e-3426.97tRNA methyltransferase 2 homolog A [Source:HGNC Sy... [more]
TRMT2A2.421e-3426.97tRNA methyltransferase 2 homolog A [Source:HGNC Sy... [more]
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BLAST of tRNA (Uracil-5-)-methyltransferase-like protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 4
Match NameE-valueIdentityDescription
trm-2A3.659e-1024.24TRna (tRNA) Methyltransferase homolog [Source:Uni... [more]
trm-2A3.659e-1024.24TRna (tRNA) Methyltransferase homolog [Source:Uni... [more]
trm-2A6.626e-1031.76TRna (tRNA) Methyltransferase homolog [Source:Uni... [more]
trm-2A3.369e-831.76TRna (tRNA) Methyltransferase homolog [Source:Uni... [more]
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BLAST of tRNA (Uracil-5-)-methyltransferase-like protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 1
Match NameE-valueIdentityDescription
CG38081.799e-2726.79gene:FBgn0036838 transcript:FBtr0075041[more]
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BLAST of tRNA (Uracil-5-)-methyltransferase-like protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
trmt2a2.293e-3628.15tRNA methyltransferase 2 homolog A [Source:ZFIN;Ac... [more]
trmt2a2.293e-3628.15tRNA methyltransferase 2 homolog A [Source:ZFIN;Ac... [more]
trmt2a2.538e-3527.69tRNA methyltransferase 2 homolog A [Source:ZFIN;Ac... [more]
trmt2b3.859e-2929.10tRNA methyltransferase 2 homolog B [Source:NCBI ge... [more]
trmt2b4.433e-2929.10tRNA methyltransferase 2 homolog B [Source:NCBI ge... [more]
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BLAST of tRNA (Uracil-5-)-methyltransferase-like protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
TRMT2B3.702e-3429.93tRNA methyltransferase 2 homolog B [Source:NCBI ge... [more]
TRMT2B6.325e-3331.57tRNA methyltransferase 2 homolog B [Source:NCBI ge... [more]
hspa12b1.968e-3127.79heat shock protein family A (Hsp70) member 12B [So... [more]
hspa12b2.024e-3127.79heat shock protein family A (Hsp70) member 12B [So... [more]
hspa12b2.026e-3127.73heat shock protein family A (Hsp70) member 12B [So... [more]
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BLAST of tRNA (Uracil-5-)-methyltransferase-like protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Trmt2b6.280e-3528.73TRM2 tRNA methyltransferase 2B [Source:MGI Symbol;... [more]
Trmt2b6.280e-3528.73TRM2 tRNA methyltransferase 2B [Source:MGI Symbol;... [more]
Trmt2a2.611e-3026.07TRM2 tRNA methyltransferase 2A [Source:MGI Symbol;... [more]
Trmt2a2.611e-3026.07TRM2 tRNA methyltransferase 2A [Source:MGI Symbol;... [more]
Trmt2a3.589e-3026.07TRM2 tRNA methyltransferase 2A [Source:MGI Symbol;... [more]
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BLAST of tRNA (Uracil-5-)-methyltransferase-like protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q5RFM7|TRM2_PONAB3.189e-3728.54tRNA (uracil(54)-C(5))-methyltransferase homolog O... [more]
sp|Q96GJ1|TRM2_HUMAN4.116e-3628.31tRNA (uracil(54)-C(5))-methyltransferase homolog O... [more]
sp|Q8BQJ6|TRM2_MOUSE4.393e-3428.73tRNA (uracil(54)-C(5))-methyltransferase homolog O... [more]
sp|Q8IZ69|TRM2A_HUMAN1.163e-3326.97tRNA (uracil-5-)-methyltransferase homolog A OS=Ho... [more]
sp|A4QP75|TRM2B_DANRE2.913e-2829.10tRNA (uracil(54)-C(5))-methyltransferase homolog-B... [more]
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BLAST of tRNA (Uracil-5-)-methyltransferase-like protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
V4A7901.825e-4131.44Uncharacterized protein OS=Lottia gigantea OX=2251... [more]
A0A087RFN02.880e-4131.74tRNA (Uracil(54)-C(5))-methyltransferase (Fragment... [more]
A0A1V4KYH38.246e-4131.67tRNA (Uracil(54)-C(5))-methyltransferase-like prot... [more]
A0A091FSJ12.991e-4031.51tRNA (Uracil(54)-C(5))-methyltransferase (Fragment... [more]
A0A093HQC04.723e-4031.51tRNA (Uracil(54)-C(5))-methyltransferase (Fragment... [more]
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BLAST of tRNA (Uracil-5-)-methyltransferase-like protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
trmt2a2.701e-3929.38tRNA methyltransferase 2 homolog A [Source:NCBI ge... [more]
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BLAST of tRNA (Uracil-5-)-methyltransferase-like protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of tRNA (Uracil-5-)-methyltransferase-like protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of tRNA (Uracil-5-)-methyltransferase-like protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO474011.513e-3027.15Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of tRNA (Uracil-5-)-methyltransferase-like protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 3
Match NameE-valueIdentityDescription
trmt2b1.642e-3229.40tRNA methyltransferase 2 homolog B [Source:NCBI ge... [more]
trmt2a1.215e-3128.34tRNA methyltransferase 2 homolog A [Source:NCBI ge... [more]
trmt2a4.674e-2227.40tRNA methyltransferase 2 homolog A [Source:NCBI ge... [more]
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BLAST of tRNA (Uracil-5-)-methyltransferase-like protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000077010.10.000e+099.77SMESG000077010.1[more]
SMESG000008614.11.701e-3028.79SMESG000008614.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30023251 ID=SMED30023251|Name=tRNA (Uracil-5-)-methyltransferase-like protein|organism=Schmidtea mediterranea sexual|type=transcript|length=1465bp
CATAATTTAAATTATTAACTGTGATCATTAAATTTTAATCAGAACTTTGA
TTTCTTCTTTTCAACATTAGAACCTTAATTGTTGATCTTATTTTTATCCA
CTGATTACATATAAACATTGTTTTTGAAATGAAAAAAATTTTAAACACTA
CAGCTCCTTTATTGAGTCTCCCTTATGATGCGCAACTAATTACCAAGCAA
TATAAAATTTTTAGTGCTATGCAAAATATTTCATCATTACTTCGAAAGTC
TAATACATTATCACCCGAAATTCTTGAATATTGTTCCAAAAACAAAGGTT
TACTATGTAAAATCGAAGATATATATAAATCGCCAGTAATTGAAGGTTAT
AGAAACAAAATGGGAGCTATAGTTGGATTTGATGAAATGGGAAATCTGAC
TGTCGGTCACGCAATGACTTTTTCAAACTTTGATAAAACGAGAAAAAAAT
TGGTTACTTTTTCACATTTTTGTGATAAATTGGATAAGAAGCATCACATT
TTAATTGCTAAATTATTTGAAAAGTTTATTAAAGAGGACTCTCAATTTAT
ACCAAATCAAAAAAAGAAGATTTCGCTATACAAAGGAATTACAATTCGTT
CTAATAGGAAAAATGAAACAATGATTAATATACATTTTCGTGCAAGATTT
ACAGGAATTGATCGAATTTTGGGCATTTCCAAAGAACTAAGTGATCAGTT
TCTAACCAGCTTGCAAAATGAAAGTATCAATGTACGATCTTTGTATTGTC
AACAAATGGAATCTCATTTTGAACATACTCATGCTCAGTTATCTTGCAAA
TTAATTTATGGAGATAAGTATCTTATGGAAAATGTCTGTGGGAAAACTTT
TTATATTAGTCCATTGTCGTTTTTTCAGGTCAATGTTGAAGTAGCGGAAA
TGATATATCAGAAAGCTAAACAGTTAGTAGAAGATTATAAAAGTAAATCT
GGGAAAAAGATTTTGTTGCTTGATTTGTGTTGTGGAACTGGCACCCTTGG
CATCATTTTGTCTGACATTGTTGAGCAAGTCATCGGAGTAGACATTGTCC
CATCTGCTATTGAGAATGCTAAATTCAACGCTAAAGCCAACAATGTAAAT
AACGTTGAACATTTTTGTAAGCCTGTTGAAGAATTTCTTCCCGAGTTTTT
GAATTCAGATATTGCTAAGGAATACGATAACGTTGTCGCTGTTCTTAATC
CCAGTAGAGACGGGCTTAGTACGGAATTTTGTGAAACTTTGCGAAGTTTA
GAATCGCTGAAAATGATTGTCTATATATCATGTCATGTAGAGGGCCAGAC
AACATTGAATATTTGCACTCTTTGTACTGTGAAAGGAGATTCTAACCCCG
AGGTTCCATTCAGTTTGTCGAAAATTATACCACTAGATCCGTTTCCTCAT
ACCACACATGTCGAGGTCATCTTTCAAATGACCCGATGAAATATCAATAA
AATTGTTGTCTTTTT
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protein sequence of SMED30023251-orf-1

>SMED30023251-orf-1 ID=SMED30023251-orf-1|Name=SMED30023251-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=437bp
MKKILNTTAPLLSLPYDAQLITKQYKIFSAMQNISSLLRKSNTLSPEILE
YCSKNKGLLCKIEDIYKSPVIEGYRNKMGAIVGFDEMGNLTVGHAMTFSN
FDKTRKKLVTFSHFCDKLDKKHHILIAKLFEKFIKEDSQFIPNQKKKISL
YKGITIRSNRKNETMINIHFRARFTGIDRILGISKELSDQFLTSLQNESI
NVRSLYCQQMESHFEHTHAQLSCKLIYGDKYLMENVCGKTFYISPLSFFQ
VNVEVAEMIYQKAKQLVEDYKSKSGKKILLLDLCCGTGTLGIILSDIVEQ
VIGVDIVPSAIENAKFNAKANNVNNVEHFCKPVEEFLPEFLNSDIAKEYD
NVVAVLNPSRDGLSTEFCETLRSLESLKMIVYISCHVEGQTTLNICTLCT
VKGDSNPEVPFSLSKIIPLDPFPHTTHVEVIFQMTR*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: molecular function
TermDefinition
GO:0003676nucleic acid binding
GO:0003723RNA binding
GO:0008173RNA methyltransferase activity
GO:0008168methyltransferase activity
GO:0016740transferase activity
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0002109X1 cell
PLANA:0002111X2 cell
Vocabulary: INTERPRO
TermDefinition
IPR029063SAM-dependent_MTases
IPR010280U5_MeTrfase_fam
IPR030391MeTrfase_TrmA_CS
Vocabulary: biological process
TermDefinition
GO:0006396RNA processing
GO:0001510RNA methylation
GO:0032259methylation
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 253..273
NoneNo IPR availableGENE3DG3DSA:3.40.50.150coord: 14..431
e-value: 6.9E-62
score: 211.4
NoneNo IPR availableGENE3DG3DSA:3.40.1350.30coord: 75..249
e-value: 6.9E-62
score: 211.4
NoneNo IPR availablePANTHERPTHR45904FAMILY NOT NAMEDcoord: 3..436
NoneNo IPR availableCDDcd02440AdoMet_MTasescoord: 280..359
e-value: 2.47005E-6
score: 43.9579
IPR010280(Uracil-5)-methyltransferase familyPFAMPF05958tRNA_U5-meth_trcoord: 229..430
e-value: 3.4E-12
score: 46.0
IPR010280(Uracil-5)-methyltransferase familyPROSITEPS51687SAM_MT_RNA_M5Ucoord: 7..436
score: 44.19
IPR030391RNA methyltransferase TrmA, conserved sitePROSITEPS01231TRMA_2coord: 419..429
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILYSSF53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 10..435