BPTI/Kunitz inhibitor domain-containing protein

Overview
NameBPTI/Kunitz inhibitor domain-containing protein
Smed IDSMED30023241
Length (bp)409
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of BPTI/Kunitz inhibitor domain-containing protein (SMED30023241) t-SNE clustered cells

Violin plots show distribution of expression levels for BPTI/Kunitz inhibitor domain-containing protein (SMED30023241) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of BPTI/Kunitz inhibitor domain-containing protein (SMED30023241) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for BPTI/Kunitz inhibitor domain-containing protein (SMED30023241) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30023241

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 7

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
prepharyngeal regionSMED30023241 SmedASXL_051938SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016
whole organism asexual adult RNA-sequencing evidence
pigment cellSMED30023241 SmedASXL_051938SmedAsxl_ww_GCZZ01PMID:29158443
He et al., 2017
whole organism asexual adult RNA-sequencing evidence
gutSMED30023241 dd_Smed_v4_190_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30023241 Contig46988uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30023241 Contig46988newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
tail regionSMED30023241 dd_Smed_v6_190_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017
whole organism asexual adult RNA-sequencing evidence
goblet cellSMED30023241 dd_Smed_v6_190_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of BPTI/Kunitz inhibitor domain-containing protein vs. Ensembl Human
Match: EPPIN (epididymal peptidase inhibitor [Source:HGNC Symbol;Acc:HGNC:15932])

HSP 1 Score: 50.447 bits (119), Expect = 2.038e-8
Identity = 24/68 (35.29%), Postives = 35/68 (51.47%), Query Frame = 1
Query:   58 LFVTILSCIECKEPECNQPLEIGLCRQYRVRYGYSVERGRCVTFTYGGCPYNDNNFFTRAECEARCEN 261
            +F     C++ K+  C  P E G C  Y + + Y  +   C  F YGGC  N+NNF ++A C   C+N
Sbjct:   46 VFSCGKKCLDLKQDVCEMPKETGPCLAYFLHWWYDKKDNTCSMFVYGGCQGNNNNFQSKANCLNTCKN 113          
BLAST of BPTI/Kunitz inhibitor domain-containing protein vs. Ensembl Human
Match: SPINT3 (serine peptidase inhibitor, Kunitz type 3 [Source:HGNC Symbol;Acc:HGNC:11248])

HSP 1 Score: 49.6766 bits (117), Expect = 2.367e-8
Identity = 22/52 (42.31%), Postives = 30/52 (57.69%), Query Frame = 1
Query:  103 CNQPLEIGLCRQYRVRYGYSVERGRCVTFTYGGCPYNDNNFFTRAECEARCE 258
            C  P+E G C+ Y  R+ ++ E G C  F YGGC  N NNF  + +CE  C+
Sbjct:   36 CAFPMEKGPCQTYMTRWFFNFETGECELFAYGGCGGNSNNFLRKEKCEKFCK 87          
BLAST of BPTI/Kunitz inhibitor domain-containing protein vs. Ensembl Human
Match: WFIKKN2 (WAP, follistatin/kazal, immunoglobulin, kunitz and netrin domain containing 2 [Source:HGNC Symbol;Acc:HGNC:30916])

HSP 1 Score: 52.7582 bits (125), Expect = 2.573e-8
Identity = 24/62 (38.71%), Postives = 35/62 (56.45%), Query Frame = 1
Query:   70 ILSCIECKEPECNQPLEIGLCRQYRVRYGYSVERGRCVTFTYGGCPYNDNNFFTRAECEARC 255
            +L+C+      C+ P   G C+ Y  R+ Y+ + G+C +F YGGC  N NNF +R  CE  C
Sbjct:  282 MLACMSGPLAACSLPALQGPCKAYAPRWAYNSQTGQCQSFVYGGCEGNGNNFESREACEESC 343          
BLAST of BPTI/Kunitz inhibitor domain-containing protein vs. Ensembl Human
Match: WFIKKN2 (WAP, follistatin/kazal, immunoglobulin, kunitz and netrin domain containing 2 [Source:HGNC Symbol;Acc:HGNC:30916])

HSP 1 Score: 52.373 bits (124), Expect = 2.918e-8
Identity = 24/62 (38.71%), Postives = 35/62 (56.45%), Query Frame = 1
Query:   70 ILSCIECKEPECNQPLEIGLCRQYRVRYGYSVERGRCVTFTYGGCPYNDNNFFTRAECEARC 255
            +L+C+      C+ P   G C+ Y  R+ Y+ + G+C +F YGGC  N NNF +R  CE  C
Sbjct:  375 MLACMSGPLAACSLPALQGPCKAYAPRWAYNSQTGQCQSFVYGGCEGNGNNFESREACEESC 436          
BLAST of BPTI/Kunitz inhibitor domain-containing protein vs. Ensembl Human
Match: EPPIN (epididymal peptidase inhibitor [Source:HGNC Symbol;Acc:HGNC:15932])

HSP 1 Score: 50.447 bits (119), Expect = 3.219e-8
Identity = 24/68 (35.29%), Postives = 35/68 (51.47%), Query Frame = 1
Query:   58 LFVTILSCIECKEPECNQPLEIGLCRQYRVRYGYSVERGRCVTFTYGGCPYNDNNFFTRAECEARCEN 261
            +F     C++ K+  C  P E G C  Y + + Y  +   C  F YGGC  N+NNF ++A C   C+N
Sbjct:   62 VFSCGKKCLDLKQDVCEMPKETGPCLAYFLHWWYDKKDNTCSMFVYGGCQGNNNNFQSKANCLNTCKN 129          
BLAST of BPTI/Kunitz inhibitor domain-containing protein vs. Ensembl Celegans
Match: mlt-11 (pep chromosome:WBcel235:V:20667209:20678513:1 gene:WBGene00012186.1 transcript:W01F3.3g.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:mlt-11)

HSP 1 Score: 55.0694 bits (131), Expect = 1.456e-9
Identity = 25/54 (46.30%), Postives = 35/54 (64.81%), Query Frame = 1
Query:   94 EPECNQPLEIGLCRQYRVRYGYSVERGRCVTFTYGGCPYNDNNFFTRAECEARC 255
            E +C QP+E G C+ +  R+ ++V+ G C  F YGGC  N N+FFT+ ECE  C
Sbjct:  850 EEKCLQPVEPGPCKNFADRWYFNVDDGTCHPFKYGGCAGNRNHFFTQKECEVHC 903          

HSP 2 Score: 48.9062 bits (115), Expect = 2.247e-7
Identity = 21/51 (41.18%), Postives = 30/51 (58.82%), Query Frame = 1
Query:  103 CNQPLEIGLCRQYRVRYGYSVERGRCVTFTYGGCPYNDNNFFTRAECEARC 255
            C  P + G+C  Y  R+ ++ + G+C  F YG C  N+NNFF R  CE +C
Sbjct: 1421 CAMPPDAGVCTNYTPRWFFNSQTGQCEQFAYGSCGGNENNFFDRNTCERKC 1471          
BLAST of BPTI/Kunitz inhibitor domain-containing protein vs. Ensembl Celegans
Match: mlt-11 (pep chromosome:WBcel235:V:20667209:20678513:1 gene:WBGene00012186.1 transcript:W01F3.3f.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:mlt-11)

HSP 1 Score: 55.0694 bits (131), Expect = 1.456e-9
Identity = 25/54 (46.30%), Postives = 35/54 (64.81%), Query Frame = 1
Query:   94 EPECNQPLEIGLCRQYRVRYGYSVERGRCVTFTYGGCPYNDNNFFTRAECEARC 255
            E +C QP+E G C+ +  R+ ++V+ G C  F YGGC  N N+FFT+ ECE  C
Sbjct:  859 EEKCLQPVEPGPCKNFADRWYFNVDDGTCHPFKYGGCAGNRNHFFTQKECEVHC 912          

HSP 2 Score: 48.9062 bits (115), Expect = 2.335e-7
Identity = 21/51 (41.18%), Postives = 30/51 (58.82%), Query Frame = 1
Query:  103 CNQPLEIGLCRQYRVRYGYSVERGRCVTFTYGGCPYNDNNFFTRAECEARC 255
            C  P + G+C  Y  R+ ++ + G+C  F YG C  N+NNFF R  CE +C
Sbjct: 1430 CAMPPDAGVCTNYTPRWFFNSQTGQCEQFAYGSCGGNENNFFDRNTCERKC 1480          
BLAST of BPTI/Kunitz inhibitor domain-containing protein vs. Ensembl Celegans
Match: mlt-11 (pep chromosome:WBcel235:V:20667209:20678513:1 gene:WBGene00012186.1 transcript:W01F3.3h.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:mlt-11)

HSP 1 Score: 55.0694 bits (131), Expect = 1.502e-9
Identity = 25/54 (46.30%), Postives = 35/54 (64.81%), Query Frame = 1
Query:   94 EPECNQPLEIGLCRQYRVRYGYSVERGRCVTFTYGGCPYNDNNFFTRAECEARC 255
            E +C QP+E G C+ +  R+ ++V+ G C  F YGGC  N N+FFT+ ECE  C
Sbjct:  859 EEKCLQPVEPGPCKNFADRWYFNVDDGTCHPFKYGGCAGNRNHFFTQKECEVHC 912          

HSP 2 Score: 48.9062 bits (115), Expect = 2.408e-7
Identity = 21/51 (41.18%), Postives = 30/51 (58.82%), Query Frame = 1
Query:  103 CNQPLEIGLCRQYRVRYGYSVERGRCVTFTYGGCPYNDNNFFTRAECEARC 255
            C  P + G+C  Y  R+ ++ + G+C  F YG C  N+NNFF R  CE +C
Sbjct: 1575 CAMPPDAGVCTNYTPRWFFNSQTGQCEQFAYGSCGGNENNFFDRNTCERKC 1625          
BLAST of BPTI/Kunitz inhibitor domain-containing protein vs. Ensembl Celegans
Match: mlt-11 (pep chromosome:WBcel235:V:20667211:20678513:1 gene:WBGene00012186.1 transcript:W01F3.3e.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:mlt-11)

HSP 1 Score: 55.0694 bits (131), Expect = 1.503e-9
Identity = 25/54 (46.30%), Postives = 35/54 (64.81%), Query Frame = 1
Query:   94 EPECNQPLEIGLCRQYRVRYGYSVERGRCVTFTYGGCPYNDNNFFTRAECEARC 255
            E +C QP+E G C+ +  R+ ++V+ G C  F YGGC  N N+FFT+ ECE  C
Sbjct: 1080 EEKCLQPVEPGPCKNFADRWYFNVDDGTCHPFKYGGCAGNRNHFFTQKECEVHC 1133          

HSP 2 Score: 48.9062 bits (115), Expect = 2.342e-7
Identity = 21/51 (41.18%), Postives = 30/51 (58.82%), Query Frame = 1
Query:  103 CNQPLEIGLCRQYRVRYGYSVERGRCVTFTYGGCPYNDNNFFTRAECEARC 255
            C  P + G+C  Y  R+ ++ + G+C  F YG C  N+NNFF R  CE +C
Sbjct: 1651 CAMPPDAGVCTNYTPRWFFNSQTGQCEQFAYGSCGGNENNFFDRNTCERKC 1701          
BLAST of BPTI/Kunitz inhibitor domain-containing protein vs. Ensembl Celegans
Match: mlt-11 (pep chromosome:WBcel235:V:20667211:20678513:1 gene:WBGene00012186.1 transcript:W01F3.3a.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:mlt-11)

HSP 1 Score: 55.0694 bits (131), Expect = 1.538e-9
Identity = 25/54 (46.30%), Postives = 35/54 (64.81%), Query Frame = 1
Query:   94 EPECNQPLEIGLCRQYRVRYGYSVERGRCVTFTYGGCPYNDNNFFTRAECEARC 255
            E +C QP+E G C+ +  R+ ++V+ G C  F YGGC  N N+FFT+ ECE  C
Sbjct: 1080 EEKCLQPVEPGPCKNFADRWYFNVDDGTCHPFKYGGCAGNRNHFFTQKECEVHC 1133          

HSP 2 Score: 48.9062 bits (115), Expect = 2.373e-7
Identity = 21/51 (41.18%), Postives = 30/51 (58.82%), Query Frame = 1
Query:  103 CNQPLEIGLCRQYRVRYGYSVERGRCVTFTYGGCPYNDNNFFTRAECEARC 255
            C  P + G+C  Y  R+ ++ + G+C  F YG C  N+NNFF R  CE +C
Sbjct: 1223 CAMPPDAGVCTNYTPRWFFNSQTGQCEQFAYGSCGGNENNFFDRNTCERKC 1273          
BLAST of BPTI/Kunitz inhibitor domain-containing protein vs. Ensembl Fly
Match: Ppn (gene:FBgn0003137 transcript:FBtr0301838)

HSP 1 Score: 55.4546 bits (132), Expect = 1.417e-9
Identity = 24/52 (46.15%), Postives = 33/52 (63.46%), Query Frame = 1
Query:  100 ECNQPLEIGLCRQYRVRYGYSVERGRCVTFTYGGCPYNDNNFFTRAECEARC 255
            +C+QP + G C ++ + + Y+   GRC +F YGGC  NDN F T  EC ARC
Sbjct: 2000 QCSQPADPGQCDKWALHWNYNETEGRCQSFYYGGCGGNDNRFATEEECSARC 2051          

HSP 2 Score: 50.0618 bits (118), Expect = 9.938e-8
Identity = 23/53 (43.40%), Postives = 29/53 (54.72%), Query Frame = 1
Query:  103 CNQPLEIGLCRQYRVRYGYSVERGRCVTFTYGGCPYNDNNFFTRAECEARCEN 261
            C  P E G C  Y V+Y +    G C  F YGGC  NDN F + AEC+  C++
Sbjct: 1612 CGLPKETGTCNNYSVKYYFDTSYGGCARFWYGGCDGNDNRFESEAECKDTCQD 1664          
BLAST of BPTI/Kunitz inhibitor domain-containing protein vs. Ensembl Fly
Match: Ppn (gene:FBgn0003137 transcript:FBtr0301839)

HSP 1 Score: 55.4546 bits (132), Expect = 1.562e-9
Identity = 24/52 (46.15%), Postives = 33/52 (63.46%), Query Frame = 1
Query:  100 ECNQPLEIGLCRQYRVRYGYSVERGRCVTFTYGGCPYNDNNFFTRAECEARC 255
            +C+QP + G C ++ + + Y+   GRC +F YGGC  NDN F T  EC ARC
Sbjct: 1943 QCSQPADPGQCDKWALHWNYNETEGRCQSFYYGGCGGNDNRFATEEECSARC 1994          

HSP 2 Score: 50.0618 bits (118), Expect = 1.054e-7
Identity = 23/53 (43.40%), Postives = 29/53 (54.72%), Query Frame = 1
Query:  103 CNQPLEIGLCRQYRVRYGYSVERGRCVTFTYGGCPYNDNNFFTRAECEARCEN 261
            C  P E G C  Y V+Y +    G C  F YGGC  NDN F + AEC+  C++
Sbjct: 1612 CGLPKETGTCNNYSVKYYFDTSYGGCARFWYGGCDGNDNRFESEAECKDTCQD 1664          
BLAST of BPTI/Kunitz inhibitor domain-containing protein vs. Ensembl Fly
Match: Ppn (gene:FBgn0003137 transcript:FBtr0301837)

HSP 1 Score: 55.4546 bits (132), Expect = 1.562e-9
Identity = 24/52 (46.15%), Postives = 33/52 (63.46%), Query Frame = 1
Query:  100 ECNQPLEIGLCRQYRVRYGYSVERGRCVTFTYGGCPYNDNNFFTRAECEARC 255
            +C+QP + G C ++ + + Y+   GRC +F YGGC  NDN F T  EC ARC
Sbjct: 2000 QCSQPADPGQCDKWALHWNYNETEGRCQSFYYGGCGGNDNRFATEEECSARC 2051          

HSP 2 Score: 50.0618 bits (118), Expect = 1.044e-7
Identity = 23/53 (43.40%), Postives = 29/53 (54.72%), Query Frame = 1
Query:  103 CNQPLEIGLCRQYRVRYGYSVERGRCVTFTYGGCPYNDNNFFTRAECEARCEN 261
            C  P E G C  Y V+Y +    G C  F YGGC  NDN F + AEC+  C++
Sbjct: 1612 CGLPKETGTCNNYSVKYYFDTSYGGCARFWYGGCDGNDNRFESEAECKDTCQD 1664          
BLAST of BPTI/Kunitz inhibitor domain-containing protein vs. Ensembl Fly
Match: CG15418 (gene:FBgn0031554 transcript:FBtr0077556)

HSP 1 Score: 50.8322 bits (120), Expect = 5.727e-9
Identity = 23/51 (45.10%), Postives = 30/51 (58.82%), Query Frame = 1
Query:  103 CNQPLEIGLCRQYRVRYGYSVERGRCVTFTYGGCPYNDNNFFTRAECEARC 255
            C QP   GLCR +++RY Y+ + G C +F Y GC   +NNF T  EC   C
Sbjct:   41 CRQPKAPGLCRGHQLRYAYNKKTGNCESFIYTGCASTENNFLTFEECRRDC 91          
BLAST of BPTI/Kunitz inhibitor domain-containing protein vs. Ensembl Fly
Match: CG15418 (gene:FBgn0031554 transcript:FBtr0446100)

HSP 1 Score: 50.8322 bits (120), Expect = 5.727e-9
Identity = 23/51 (45.10%), Postives = 30/51 (58.82%), Query Frame = 1
Query:  103 CNQPLEIGLCRQYRVRYGYSVERGRCVTFTYGGCPYNDNNFFTRAECEARC 255
            C QP   GLCR +++RY Y+ + G C +F Y GC   +NNF T  EC   C
Sbjct:   41 CRQPKAPGLCRGHQLRYAYNKKTGNCESFIYTGCASTENNFLTFEECRRDC 91          
BLAST of BPTI/Kunitz inhibitor domain-containing protein vs. Ensembl Zebrafish
Match: col28a2a (collagen, type XXVIII, alpha 2a [Source:ZFIN;Acc:ZDB-GENE-030131-4444])

HSP 1 Score: 53.5286 bits (127), Expect = 8.728e-9
Identity = 22/54 (40.74%), Postives = 27/54 (50.00%), Query Frame = 1
Query:   94 EPECNQPLEIGLCRQYRVRYGYSVERGRCVTFTYGGCPYNDNNFFTRAECEARC 255
            +P C      G CR Y +R+ Y  +   C  F YGGC  NDN F T  EC+  C
Sbjct: 1133 DPRCQLSFVQGSCRNYVIRWYYEKQSNSCAQFWYGGCDGNDNRFHTEEECKTTC 1186          
BLAST of BPTI/Kunitz inhibitor domain-containing protein vs. Ensembl Zebrafish
Match: col28a2a (collagen, type XXVIII, alpha 2a [Source:ZFIN;Acc:ZDB-GENE-030131-4444])

HSP 1 Score: 53.5286 bits (127), Expect = 8.992e-9
Identity = 22/54 (40.74%), Postives = 27/54 (50.00%), Query Frame = 1
Query:   94 EPECNQPLEIGLCRQYRVRYGYSVERGRCVTFTYGGCPYNDNNFFTRAECEARC 255
            +P C      G CR Y +R+ Y  +   C  F YGGC  NDN F T  EC+  C
Sbjct: 1149 DPRCQLSFVQGSCRNYVIRWYYEKQSNSCAQFWYGGCDGNDNRFHTEEECKTTC 1202          
BLAST of BPTI/Kunitz inhibitor domain-containing protein vs. Ensembl Zebrafish
Match: col6a4a (collagen, type VI, alpha 4a [Source:ZFIN;Acc:ZDB-GENE-041111-303])

HSP 1 Score: 51.9878 bits (123), Expect = 3.353e-8
Identity = 21/51 (41.18%), Postives = 29/51 (56.86%), Query Frame = 1
Query:  103 CNQPLEIGLCRQYRVRYGYSVERGRCVTFTYGGCPYNDNNFFTRAECEARC 255
            C    ++G C  Y + + Y +++  C  F +GGC  N N F TRAECEA C
Sbjct: 2486 CLMKQDVGPCSNYVLSWYYDIQQNECSQFWFGGCEGNKNRFETRAECEALC 2536          
BLAST of BPTI/Kunitz inhibitor domain-containing protein vs. Ensembl Zebrafish
Match: si:ch211-62a1.3 (si:ch211-62a1.3 [Source:ZFIN;Acc:ZDB-GENE-061207-41])

HSP 1 Score: 47.7506 bits (112), Expect = 8.912e-8
Identity = 19/44 (43.18%), Postives = 28/44 (63.64%), Query Frame = 1
Query:  124 GLCRQYRVRYGYSVERGRCVTFTYGGCPYNDNNFFTRAECEARC 255
            G C  + +++ ++   G+CV F YGGC  NDN F T+ +CE RC
Sbjct:   28 GSCSNFTLKWAFNSRNGQCVQFWYGGCEGNDNRFNTQEDCEIRC 71          
BLAST of BPTI/Kunitz inhibitor domain-containing protein vs. Ensembl Zebrafish
Match: spint1b (serine peptidase inhibitor, Kunitz type 1 b [Source:ZFIN;Acc:ZDB-GENE-071218-1])

HSP 1 Score: 50.0618 bits (118), Expect = 1.143e-7
Identity = 23/66 (34.85%), Postives = 34/66 (51.52%), Query Frame = 1
Query:   64 VTILSCIECKEPECNQPLEIGLCRQYRVRYGYSVERGRCVTFTYGGCPYNDNNFFTRAECEARCEN 261
            + ++   E  E  C  P + G CR   +R+ Y+    RC  F +GGC  N NN+ +  EC+  CEN
Sbjct:  222 IVLVLTSEQSERHCLTPKKTGPCRASFIRWNYNAASRRCEQFIFGGCMENSNNYLSETECQNACEN 287          
BLAST of BPTI/Kunitz inhibitor domain-containing protein vs. Ensembl Xenopus
Match: WFIKKN2 (WAP, follistatin/kazal, immunoglobulin, kunitz and netrin domain containing 2 [Source:NCBI gene;Acc:100486381])

HSP 1 Score: 55.0694 bits (131), Expect = 3.093e-9
Identity = 25/62 (40.32%), Postives = 37/62 (59.68%), Query Frame = 1
Query:   70 ILSCIECKEPECNQPLEIGLCRQYRVRYGYSVERGRCVTFTYGGCPYNDNNFFTRAECEARC 255
            +L+C+      CN P   G+C+ Y+ R+ Y+ +  +C +F YGGC  NDNNF T+  CE  C
Sbjct:  359 MLTCMNGPVNVCNFPALQGMCKAYKPRWAYNKQLKQCQSFIYGGCGGNDNNFETKETCEDMC 420          
BLAST of BPTI/Kunitz inhibitor domain-containing protein vs. Ensembl Xenopus
Match: cmtr2 (cap methyltransferase 2 [Source:Xenbase;Acc:XB-GENE-6035233])

HSP 1 Score: 50.0618 bits (118), Expect = 9.304e-9
Identity = 19/48 (39.58%), Postives = 28/48 (58.33%), Query Frame = 1
Query:  103 CNQPLEIGLCRQYRVRYGYSVERGRCVTFTYGGCPYNDNNFFTRAECE 246
            C  P+  G C+  +  + +   +G+CVTF YGGC  N N F+T  EC+
Sbjct:    6 CRLPITPGPCKAAKTHWAFDAAQGKCVTFAYGGCQGNGNQFYTEKECK 53          
BLAST of BPTI/Kunitz inhibitor domain-containing protein vs. Ensembl Xenopus
Match: cmtr2 (cap methyltransferase 2 [Source:Xenbase;Acc:XB-GENE-6035233])

HSP 1 Score: 49.6766 bits (117), Expect = 1.296e-8
Identity = 19/51 (37.25%), Postives = 28/51 (54.90%), Query Frame = 1
Query:   94 EPECNQPLEIGLCRQYRVRYGYSVERGRCVTFTYGGCPYNDNNFFTRAECE 246
               C  P+  G C+  +  + +   +G+CVTF YGGC  N N F+T  EC+
Sbjct:    3 HAACRLPITPGPCKAAKTHWAFDAAQGKCVTFAYGGCQGNGNQFYTEKECK 53          
BLAST of BPTI/Kunitz inhibitor domain-containing protein vs. Ensembl Xenopus
Match: itch (itchy E3 ubiquitin protein ligase [Source:Xenbase;Acc:XB-GENE-976485])

HSP 1 Score: 52.373 bits (124), Expect = 3.423e-8
Identity = 24/54 (44.44%), Postives = 30/54 (55.56%), Query Frame = 1
Query:   97 PE-CNQPLEIGLCRQYRVRYGYSVERGRCVTFTYGGCPYNDNNFFTRAECEARC 255
            PE CN   E G CR + +++ Y  E   C  F YGGC  N+N F T+ ECE  C
Sbjct: 2826 PEVCNLQKEEGTCRDFVLKWYYDPETKSCARFWYGGCGGNENRFSTQKECEKIC 2879          
BLAST of BPTI/Kunitz inhibitor domain-containing protein vs. Ensembl Xenopus
Match: itch (itchy E3 ubiquitin protein ligase [Source:Xenbase;Acc:XB-GENE-976485])

HSP 1 Score: 52.373 bits (124), Expect = 3.428e-8
Identity = 24/54 (44.44%), Postives = 30/54 (55.56%), Query Frame = 1
Query:   97 PE-CNQPLEIGLCRQYRVRYGYSVERGRCVTFTYGGCPYNDNNFFTRAECEARC 255
            PE CN   E G CR + +++ Y  E   C  F YGGC  N+N F T+ ECE  C
Sbjct: 3006 PEVCNLQKEEGTCRDFVLKWYYDPETKSCARFWYGGCGGNENRFSTQKECEKIC 3059          
BLAST of BPTI/Kunitz inhibitor domain-containing protein vs. Ensembl Mouse
Match: Spint3 (serine peptidase inhibitor, Kunitz type, 3 [Source:MGI Symbol;Acc:MGI:3651470])

HSP 1 Score: 57.7658 bits (138), Expect = 1.213e-11
Identity = 33/81 (40.74%), Postives = 46/81 (56.79%), Query Frame = 1
Query:   52 FLLFVTILSCIE-CKEPE----------CNQPLEIGLCRQYRVRYGYSVERGRCVTFTYGGCPYNDNNFFTRAECEARCEN 261
            F LF+ ++ C E C EP           C  P +IG CR   VR+ Y+ + G+C  F YGGC  N+NNF +R++C+A C N
Sbjct:    7 FFLFLILIFCQELCAEPNNVPRKSLPPMCILPKDIGGCRAVFVRWYYNSKTGKCEWFRYGGCKGNENNFPSRSQCQAVCTN 87          
BLAST of BPTI/Kunitz inhibitor domain-containing protein vs. Ensembl Mouse
Match: Wfdc6b (WAP four-disulfide core domain 6B [Source:MGI Symbol;Acc:MGI:3575430])

HSP 1 Score: 49.6766 bits (117), Expect = 4.628e-8
Identity = 25/68 (36.76%), Postives = 36/68 (52.94%), Query Frame = 1
Query:   58 LFVTILSCIECKEPECNQPLEIGLCRQYRVRYGYSVERGRCVTFTYGGCPYNDNNFFTRAECEARCEN 261
            LF     C++  E  C+ P + G C  Y  R+ Y+ +   C+ F YGGC  N NNF ++A C + C N
Sbjct:   73 LFACGRKCLDLSEDICSLPQDAGPCLAYLPRWWYNQDTKLCIEFIYGGCQGNPNNFESKAVCTSICIN 140          
BLAST of BPTI/Kunitz inhibitor domain-containing protein vs. Ensembl Mouse
Match: Wfikkn2 (WAP, follistatin/kazal, immunoglobulin, kunitz and netrin domain containing 2 [Source:MGI Symbol;Acc:MGI:2669209])

HSP 1 Score: 50.0618 bits (118), Expect = 1.273e-7
Identity = 24/62 (38.71%), Postives = 34/62 (54.84%), Query Frame = 1
Query:   70 ILSCIECKEPECNQPLEIGLCRQYRVRYGYSVERGRCVTFTYGGCPYNDNNFFTRAECEARC 255
            +L+C+      C+ P   G C+ Y  R+ Y+ + G C +F YGGC  N NNF +R  CE  C
Sbjct:  370 MLACMSGPLAICSLPALQGPCKAYVPRWAYNSQTGLCQSFVYGGCEGNGNNFESREACEESC 431          
BLAST of BPTI/Kunitz inhibitor domain-containing protein vs. Ensembl Mouse
Match: Aplp2 (amyloid beta (A4) precursor-like protein 2 [Source:MGI Symbol;Acc:MGI:88047])

HSP 1 Score: 47.7506 bits (112), Expect = 8.369e-7
Identity = 21/52 (40.38%), Postives = 29/52 (55.77%), Query Frame = 1
Query:  103 CNQPLEIGLCRQYRVRYGYSVERGRCVTFTYGGCPYNDNNFFTRAECEARCE 258
            C+Q    G CR    R+ + + +G+CV F YGGC  N NNF +   C A C+
Sbjct:  310 CSQEAMTGPCRAVMPRWYFDLSKGKCVRFIYGGCGGNRNNFESEDYCMAVCK 361          
BLAST of BPTI/Kunitz inhibitor domain-containing protein vs. Ensembl Mouse
Match: Ambp (alpha 1 microglobulin/bikunin precursor [Source:MGI Symbol;Acc:MGI:88002])

HSP 1 Score: 47.3654 bits (111), Expect = 1.014e-6
Identity = 19/51 (37.25%), Postives = 29/51 (56.86%), Query Frame = 1
Query:  103 CNQPLEIGLCRQYRVRYGYSVERGRCVTFTYGGCPYNDNNFFTRAECEARC 255
            CN P+  G CR +   + +   +G+C+ F YGGC  N N F++  EC+  C
Sbjct:  286 CNLPIVQGPCRAFIKLWAFDAAQGKCIQFHYGGCKGNGNKFYSEKECKEYC 336          
BLAST of BPTI/Kunitz inhibitor domain-containing protein vs. UniProt/SwissProt
Match: sp|D8KY58|VKT_BOMTE (Kunitz-type serine protease inhibitor Bt-KTI OS=Bombus terrestris OX=30195 PE=2 SV=1)

HSP 1 Score: 57.3806 bits (137), Expect = 1.104e-10
Identity = 27/53 (50.94%), Postives = 31/53 (58.49%), Query Frame = 1
Query:  100 ECNQPLEIGLCRQYRVRYGYSVERGRCVTFTYGGCPYNDNNFFTRAECEARCE 258
             C  PL  G CR Y  R+GY+VE G+CV F YGGC  N NNF    EC+  C 
Sbjct:   29 HCTLPLATGTCRGYFPRFGYNVEMGKCVEFIYGGCDGNANNFRNLEECQQSCS 81          
BLAST of BPTI/Kunitz inhibitor domain-containing protein vs. UniProt/SwissProt
Match: sp|P0DJ50|VKT31_MESMA (Kunitz-type serine protease inhibitor BmKTT-2 OS=Mesobuthus martensii OX=34649 PE=2 SV=2)

HSP 1 Score: 54.6842 bits (130), Expect = 1.014e-9
Identity = 22/72 (30.56%), Postives = 41/72 (56.94%), Query Frame = 1
Query:   43 SIIFLLFVTILSCIECKEPECNQPLEIGLCRQYRVRYGYSVERGRCVTFTYGGCPYNDNNFFTRAECEARCE 258
            +++ ++   + +  + +  +C  P + G C+ Y +RY Y+ + G C  F YGGC  N NNF T+++C  +C 
Sbjct:    6 TVVGIILSVVCTISDAEGVDCTLPSDTGRCKAYFIRYFYNQKAGECQKFVYGGCEGNSNNFLTKSDCCKQCS 77          
BLAST of BPTI/Kunitz inhibitor domain-containing protein vs. UniProt/SwissProt
Match: sp|P0DMJ3|VKTI1_ANTFU (PI-actitoxin-Afv2a OS=Anthopleura fuscoviridis OX=6111 PE=1 SV=1)

HSP 1 Score: 54.299 bits (129), Expect = 1.078e-9
Identity = 24/52 (46.15%), Postives = 33/52 (63.46%), Query Frame = 1
Query:  100 ECNQPLEIGLCRQYRVRYGYSVERGRCVTFTYGGCPYNDNNFFTRAECEARC 255
             C  P+++G CR +  R+ Y+   G+C  FTYGGC  N NNF T+A+CE  C
Sbjct:    4 NCLLPMKVGFCRAHVPRFYYNSSSGKCEGFTYGGCGANANNFQTKAQCEKAC 55          
BLAST of BPTI/Kunitz inhibitor domain-containing protein vs. UniProt/SwissProt
Match: sp|Q589G4|VKT19_ANOSM (Kunitz-type serine protease inhibitor As-fr-19 OS=Anoplius samariensis OX=200614 PE=1 SV=1)

HSP 1 Score: 54.299 bits (129), Expect = 1.334e-9
Identity = 28/72 (38.89%), Postives = 39/72 (54.17%), Query Frame = 1
Query:   49 IFLLFVTILSCIECKEPE--CNQPLEIGLCRQYRVRYGYSVERGRCVTFTYGGCPYNDNNFFTRAECEARCE 258
            + LL ++I + ++       C  P+  G C QY +RY +      C  FT+GGC  NDNNF TR +CE  CE
Sbjct:    1 MMLLVLSISAILQVSHSVSFCLLPIVPGPCTQYVIRYAFQPSISACRRFTFGGCEGNDNNFMTRRDCEHYCE 72          
BLAST of BPTI/Kunitz inhibitor domain-containing protein vs. UniProt/SwissProt
Match: sp|Q8T0W4|VKT2_PIMHY (Kunitz-type serine protease inhibitor cvp2 OS=Pimpla hypochondriaca OX=135724 PE=1 SV=1)

HSP 1 Score: 53.5286 bits (127), Expect = 2.689e-9
Identity = 27/70 (38.57%), Postives = 40/70 (57.14%), Query Frame = 1
Query:   46 IIFLLFVTILSCIECKEPECNQPLEIGLCRQYRVRYGYSVERGRCVTFTYGGCPYNDNNFFTRAECEARC 255
            +I + FV + +     +P C+    +GLC+    R+  +   G+C  F YGGC  N NNF T+AECEA+C
Sbjct:    9 LIVVGFVGMFNVATAADPLCSLEPAVGLCKASIPRF--ASVGGKCQEFIYGGCGGNANNFQTQAECEAKC 76          
BLAST of BPTI/Kunitz inhibitor domain-containing protein vs. TrEMBL
Match: A0A1I8NL51 (BPTI/Kunitz inhibitor domain-containing protein OS=Musca domestica OX=7370 GN=105261756 PE=4 SV=1)

HSP 1 Score: 69.707 bits (169), Expect = 4.574e-13
Identity = 31/64 (48.44%), Postives = 42/64 (65.62%), Query Frame = 1
Query:   70 ILSCIECKEPECNQP-LEIGLCRQYRVRYGYSVERGRCVTFTYGGCPYNDNNFFTRAECEARCE 258
            +LS    K+P C+QP +EIG CR  + R+ Y  +   CV F YGGC  NDNNF T++ECE +C+
Sbjct:   14 VLSVAAAKDPACDQPPMEIGQCRATQKRFTYVAKSNECVAFNYGGCRGNDNNFATKSECEKKCK 77          
BLAST of BPTI/Kunitz inhibitor domain-containing protein vs. TrEMBL
Match: A0A0R3WAZ9 (Uncharacterized protein OS=Taenia asiatica OX=60517 GN=TASK_LOCUS7770 PE=4 SV=1)

HSP 1 Score: 70.0922 bits (170), Expect = 6.470e-12
Identity = 32/56 (57.14%), Postives = 37/56 (66.07%), Query Frame = 1
Query:   91 KEPECNQPLEIGLCRQYRVRYGYSVERGRCVTFTYGGCPYNDNNFFTRAECEARCE 258
            KE  CN PL+IGLCR Y   +GY+   G CV F YGGC  N+NNFFT+ EC   CE
Sbjct:   47 KEDICNLPLDIGLCRAYMEVWGYNSTAGHCVKFVYGGCGGNENNFFTKKECRQTCE 102          
BLAST of BPTI/Kunitz inhibitor domain-containing protein vs. TrEMBL
Match: A0A0R3WBP8 (BPTI/Kunitz inhibitor domain-containing protein OS=Taenia asiatica OX=60517 GN=TASK_LOCUS8078 PE=4 SV=1)

HSP 1 Score: 65.855 bits (159), Expect = 2.415e-11
Identity = 30/73 (41.10%), Postives = 41/73 (56.16%), Query Frame = 1
Query:   37 MKSIIFLLFVTILSCIECKEPECNQPLEIGLCRQYRVRYGYSVERGRCVTFTYGGCPYNDNNFFTRAECEARC 255
            M + + L  +  +S  E +   C  P+E GLCR Y  R+GY  E G CV F YGGC  N N+F ++ +CE  C
Sbjct:   21 MIATLALFVLVAISLTEARIDPCKLPIEPGLCRAYMPRWGYDQESGECVRFIYGGCGGNKNHFHSKEQCENMC 93          
BLAST of BPTI/Kunitz inhibitor domain-containing protein vs. TrEMBL
Match: A0A2L2Y572 (Microlepidin-1 (Fragment) OS=Parasteatoda tepidariorum OX=114398 PE=2 SV=1)

HSP 1 Score: 65.0846 bits (157), Expect = 2.530e-11
Identity = 31/77 (40.26%), Postives = 44/77 (57.14%), Query Frame = 1
Query:   34 TMKSIIFLLF--VTILSCIECKEPECNQPLEIGLCRQYRVRYGYSVERGRCVTFTYGGCPYNDNNFFTRAECEARCE 258
            T+   +FLLF  + I+S     +  CN P+  G+CR    R+GY   +G+C  F YGGC  N NNF +  EC+ +CE
Sbjct:    1 TIMKYLFLLFCAICIISMARGADDACNDPITTGMCRAAMPRFGYDKSQGKCRGFIYGGCGANGNNFKSLEECQNKCE 77          
BLAST of BPTI/Kunitz inhibitor domain-containing protein vs. TrEMBL
Match: A0A3P8DGB2 (BPTI/Kunitz inhibitor domain-containing protein OS=Heligmosomoides polygyrus OX=6339 GN=HPBE_LOCUS22501 PE=4 SV=1)

HSP 1 Score: 64.6994 bits (156), Expect = 3.745e-11
Identity = 30/70 (42.86%), Postives = 42/70 (60.00%), Query Frame = 1
Query:   46 IIFLLFVTILSCIECKEPECNQPLEIGLCRQYRVRYGYSVERGRCVTFTYGGCPYNDNNFFTRAECEARC 255
            ++  + V +++ + C E  C   LE G CR   +RYGYS   G+CV F YGGC  N+NNF T  EC+ +C
Sbjct:    7 LLLAVIVVLMTIVYCDEI-CKMKLETGPCRMRDIRYGYSTAEGKCVKFDYGGCKGNENNFETIEECQKKC 75          
BLAST of BPTI/Kunitz inhibitor domain-containing protein vs. Ensembl Cavefish
Match: col28a2a (collagen alpha-1(XXVIII) chain-like [Source:NCBI gene;Acc:103029985])

HSP 1 Score: 54.6842 bits (130), Expect = 3.176e-9
Identity = 23/54 (42.59%), Postives = 30/54 (55.56%), Query Frame = 1
Query:   94 EPECNQPLEIGLCRQYRVRYGYSVERGRCVTFTYGGCPYNDNNFFTRAECEARC 255
            +P C+  L  G CR+Y +R+ Y      C  F YGGC  N+N F T AEC+  C
Sbjct: 1191 DPRCSLSLNQGPCREYLIRWYYDKAANSCAQFWYGGCEGNENRFETEAECKKTC 1244          
BLAST of BPTI/Kunitz inhibitor domain-containing protein vs. Ensembl Cavefish
Match: wfikkn2b (WAP, Kazal, immunoglobulin, Kunitz and NTR domain-containing protein 2 [Source:NCBI gene;Acc:103024700])

HSP 1 Score: 52.7582 bits (125), Expect = 1.219e-8
Identity = 27/62 (43.55%), Postives = 34/62 (54.84%), Query Frame = 1
Query:   70 ILSCIECKEPECNQPLEIGLCRQYRVRYGYSVERGRCVTFTYGGCPYNDNNFFTRAECEARC 255
            +LSC       C+ P   G C+ Y  R+ YS+  G+C  F YGGC  N+NNF TR  CE  C
Sbjct:  363 LLSCQGELAVPCSLPSLQGPCKAYEPRWAYSITLGQCQPFIYGGCEGNENNFETRETCEDSC 424          
BLAST of BPTI/Kunitz inhibitor domain-containing protein vs. Ensembl Cavefish
Match: col28a2b (collagen, type XXVIII, alpha 2b [Source:ZFIN;Acc:ZDB-GENE-130530-762])

HSP 1 Score: 52.7582 bits (125), Expect = 1.302e-8
Identity = 22/54 (40.74%), Postives = 29/54 (53.70%), Query Frame = 1
Query:   94 EPECNQPLEIGLCRQYRVRYGYSVERGRCVTFTYGGCPYNDNNFFTRAECEARC 255
            +P C   L+ G CR+Y +R+ Y  +   C  F YGGC  N N F T  EC+  C
Sbjct: 1080 DPRCELTLDQGPCREYNIRWYYDKQANACAQFWYGGCDGNRNRFDTEEECKKTC 1133          
BLAST of BPTI/Kunitz inhibitor domain-containing protein vs. Ensembl Cavefish
Match: ENSAMXT00000056342.1 (pep primary_assembly:Astyanax_mexicanus-2.0:25:41015233:41016395:-1 gene:ENSAMXG00000036709.1 transcript:ENSAMXT00000056342.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 48.9062 bits (115), Expect = 2.693e-8
Identity = 22/65 (33.85%), Postives = 34/65 (52.31%), Query Frame = 1
Query:   61 FVTILSCIECKEPECNQPLEIGLCRQYRVRYGYSVERGRCVTFTYGGCPYNDNNFFTRAECEARC 255
             VT ++ ++     C  P ++G C  + +++ +      C  F YGGC  N N F T+ ECEARC
Sbjct:   23 MVTAIASLQRLTLHCKLPHDLGKCYNFALKWHFDTSIQECTRFWYGGCGGNGNRFETQEECEARC 87          
BLAST of BPTI/Kunitz inhibitor domain-containing protein vs. Ensembl Cavefish
Match: wfikkn1 (WAP, follistatin/kazal, immunoglobulin, kunitz and netrin domain containing 1 [Source:NCBI gene;Acc:103037683])

HSP 1 Score: 51.2174 bits (121), Expect = 3.882e-8
Identity = 22/51 (43.14%), Postives = 30/51 (58.82%), Query Frame = 1
Query:  103 CNQPLEIGLCRQYRVRYGYSVERGRCVTFTYGGCPYNDNNFFTRAECEARC 255
            C  P   G CR +  R+ Y+    +C  F YGGC  N+NNF ++ ECE+RC
Sbjct:  377 CTLPAVQGPCRNWEARWAYNTINKQCQAFVYGGCQGNNNNFRSKVECESRC 427          
BLAST of BPTI/Kunitz inhibitor domain-containing protein vs. Ensembl Sea Lamprey
Match: WFIKKN1 (WAP, follistatin/kazal, immunoglobulin, kunitz and netrin domain containing 1 [Source:HGNC Symbol;Acc:HGNC:30912])

HSP 1 Score: 50.0618 bits (118), Expect = 2.184e-8
Identity = 22/61 (36.07%), Postives = 37/61 (60.66%), Query Frame = 1
Query:   73 LSCIECKEPECNQPLEIGLCRQYRVRYGYSVERGRCVTFTYGGCPYNDNNFFTRAECEARC 255
            ++C+      C+ P++ G C+Q+ +R+ Y+    RC +F YGGC  N+NNF ++  CE  C
Sbjct:  364 MACMANINNTCSLPVQQGPCKQWALRWAYNPLIKRCQSFIYGGCEGNENNFQSQELCEDNC 424          
BLAST of BPTI/Kunitz inhibitor domain-containing protein vs. Ensembl Sea Lamprey
Match: ENSPMAT00000008261.1 (pep scaffold:Pmarinus_7.0:GL478901:735:1448:1 gene:ENSPMAG00000007479.1 transcript:ENSPMAT00000008261.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 45.4394 bits (106), Expect = 6.014e-7
Identity = 22/60 (36.67%), Postives = 32/60 (53.33%), Query Frame = 1
Query:   76 SCIECKEPECNQPLEIGLCRQYRVRYGYSVERGRCVTFTYGGCPYNDNNFFTRAECEARC 255
            +C+      C+ P   G C+ +  R+ Y+    RC TF YGGC  N N+F +R+ CE  C
Sbjct:   12 ACVNETARVCSLPPVQGPCKAWETRWSYNPLGKRCATFLYGGCEGNGNSFASRSACERAC 71          
BLAST of BPTI/Kunitz inhibitor domain-containing protein vs. Ensembl Sea Lamprey
Match: tfpi2 (tissue factor pathway inhibitor 2 [Source:ZFIN;Acc:ZDB-GENE-060503-38])

HSP 1 Score: 45.4394 bits (106), Expect = 6.152e-7
Identity = 26/77 (33.77%), Postives = 40/77 (51.95%), Query Frame = 1
Query:   37 MKSIIFLLFVTILSCI---ECKEPECNQPLEIGLCRQYRVRYGYSVERGRCVTFTYGGCPYNDNNFFTRAECEARCE 258
            ++S +  ++++   C+   E +   C  P + G CR    RY ++    RC +F YGGC  N+NNF T  EC A C 
Sbjct:    2 VQSAVQCMYLSESQCVFTLEDQRVTCTLPAKPGDCRAAFPRYFFNRSTLRCESFIYGGCHSNENNFETMQECRAACS 78          
BLAST of BPTI/Kunitz inhibitor domain-containing protein vs. Ensembl Sea Lamprey
Match: ENSPMAT00000005043.1 (pep scaffold:Pmarinus_7.0:GL481015:31246:35903:1 gene:ENSPMAG00000004579.1 transcript:ENSPMAT00000005043.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 42.743 bits (99), Expect = 5.554e-6
Identity = 24/52 (46.15%), Postives = 28/52 (53.85%), Query Frame = 1
Query:  100 ECNQPLEIGLCRQYRVRYGYSVERGRCVTFTYGGCPYNDNNFFTRAECEARC 255
             C   L+ G CR Y  R  Y+    RC  F YGGC  N NNF + AEC A+C
Sbjct:    2 SCMMDLDPGPCRAYLPRVFYNRYTQRCEPFHYGGCFGNGNNFESLAECNAKC 53          

HSP 2 Score: 42.3578 bits (98), Expect = 7.020e-6
Identity = 21/51 (41.18%), Postives = 27/51 (52.94%), Query Frame = 1
Query:  103 CNQPLEIGLCRQYRVRYGYSVERGRCVTFTYGGCPYNDNNFFTRAECEARC 255
            C   +E+G+CR    RY +++  G C  F YGGC  N NNF   A C   C
Sbjct:   74 CRLDMEVGVCRALLRRYFFNMTSGSCEEFGYGGCQGNLNNFKDAASCYQHC 124          
BLAST of BPTI/Kunitz inhibitor domain-containing protein vs. Ensembl Sea Lamprey
Match: ENSPMAT00000005038.1 (pep scaffold:Pmarinus_7.0:GL481015:31246:35903:1 gene:ENSPMAG00000004579.1 transcript:ENSPMAT00000005038.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 42.743 bits (99), Expect = 6.147e-6
Identity = 24/52 (46.15%), Postives = 28/52 (53.85%), Query Frame = 1
Query:  100 ECNQPLEIGLCRQYRVRYGYSVERGRCVTFTYGGCPYNDNNFFTRAECEARC 255
             C   L+ G CR Y  R  Y+    RC  F YGGC  N NNF + AEC A+C
Sbjct:    2 SCMMDLDPGPCRAYLPRVFYNRYTQRCEPFHYGGCFGNGNNFESLAECNAKC 53          

HSP 2 Score: 42.3578 bits (98), Expect = 6.911e-6
Identity = 21/51 (41.18%), Postives = 27/51 (52.94%), Query Frame = 1
Query:  103 CNQPLEIGLCRQYRVRYGYSVERGRCVTFTYGGCPYNDNNFFTRAECEARC 255
            C   +E+G+CR    RY +++  G C  F YGGC  N NNF   A C   C
Sbjct:   75 CRLDMEVGVCRALLRRYFFNMTSGSCEEFGYGGCQGNLNNFKDAASCYQHC 125          
BLAST of BPTI/Kunitz inhibitor domain-containing protein vs. Ensembl Nematostella
Match: EDO41696 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S3T1])

HSP 1 Score: 50.447 bits (119), Expect = 9.965e-10
Identity = 25/52 (48.08%), Postives = 31/52 (59.62%), Query Frame = 1
Query:  100 ECNQPLEIGLCRQYRVRYGYSVERGRCVTFTYGGCPYNDNNFFTRAECEARC 255
            EC QP   G CR Y  R+ Y+    +C  F YGGC  N NNF ++AECE +C
Sbjct:    1 ECLQPKLTGPCRAYFERWFYNQTSRKCKQFVYGGCQGNSNNFESKAECEKKC 52          
BLAST of BPTI/Kunitz inhibitor domain-containing protein vs. Ensembl Nematostella
Match: EDO26263 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S3T1])

HSP 1 Score: 50.447 bits (119), Expect = 9.965e-10
Identity = 25/52 (48.08%), Postives = 31/52 (59.62%), Query Frame = 1
Query:  100 ECNQPLEIGLCRQYRVRYGYSVERGRCVTFTYGGCPYNDNNFFTRAECEARC 255
            EC QP   G CR Y  R+ Y+    +C  F YGGC  N NNF ++AECE +C
Sbjct:    1 ECLQPKLTGPCRAYFERWFYNQTSRKCKQFVYGGCQGNSNNFESKAECEKKC 52          
BLAST of BPTI/Kunitz inhibitor domain-containing protein vs. Ensembl Nematostella
Match: EDO31759 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SX08])

HSP 1 Score: 47.7506 bits (112), Expect = 1.115e-8
Identity = 22/51 (43.14%), Postives = 30/51 (58.82%), Query Frame = 1
Query:  103 CNQPLEIGLCRQYRVRYGYSVERGRCVTFTYGGCPYNDNNFFTRAECEARC 255
            C  P   G CR    ++ Y+ +R RC+ F YGGC  N NNF T+ EC++ C
Sbjct:    2 CKMPAAAGPCRAAMPQWYYNFKRHRCIRFIYGGCGGNPNNFDTKNECKSAC 52          
BLAST of BPTI/Kunitz inhibitor domain-containing protein vs. Ensembl Nematostella
Match: EDO29819 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T2J6])

HSP 1 Score: 45.8246 bits (107), Expect = 9.229e-8
Identity = 21/51 (41.18%), Postives = 28/51 (54.90%), Query Frame = 1
Query:  103 CNQPLEIGLCRQYRVRYGYSVERGRCVTFTYGGCPYNDNNFFTRAECEARC 255
            C  P ++G CR    R  ++ + G+C  F YGGC  N NNF T  EC+  C
Sbjct:   14 CTLPKKVGDCRAAMPRVFFNSQSGKCEDFIYGGCGGNANNFHTMEECKKAC 64          
BLAST of BPTI/Kunitz inhibitor domain-containing protein vs. Ensembl Nematostella
Match: EDO38284 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SDB9])

HSP 1 Score: 45.8246 bits (107), Expect = 9.822e-8
Identity = 22/51 (43.14%), Postives = 29/51 (56.86%), Query Frame = 1
Query:  103 CNQPLEIGLCRQYRVRYGYSVERGRCVTFTYGGCPYNDNNFFTRAECEARC 255
            C  P   G C     R+ Y+ + G+C +F +GGC  N NNF TR  CEA+C
Sbjct:   13 CKLPKFAGPCMASIERFYYNAKIGKCQSFVFGGCLPNGNNFKTRKACEAKC 63          
BLAST of BPTI/Kunitz inhibitor domain-containing protein vs. Ensembl Medaka
Match: ENSORLT00000042019.1 (pep primary_assembly:ASM223467v1:12:15740119:15780496:1 gene:ENSORLG00000010354.2 transcript:ENSORLT00000042019.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 52.7582 bits (125), Expect = 7.298e-9
Identity = 28/58 (48.28%), Postives = 33/58 (56.90%), Query Frame = 1
Query:   79 CIECKEPECNQPLEIGLCRQYRVRYGYSVERGRCVTFTYGGCPYNDNNFFTRAECEAR 252
            CI  K P+ + P + GLC  Y  RY Y+  +GRC  F YGGC  N NNF T  EC  R
Sbjct:   73 CILTKLPQPHIPPDSGLCFGYFERYFYNFRKGRCEIFIYGGCLGNQNNFLTEKECLQR 130          

HSP 2 Score: 46.9802 bits (110), Expect = 1.002e-6
Identity = 24/47 (51.06%), Postives = 27/47 (57.45%), Query Frame = 1
Query:  103 CNQPLEIGLCRQYRVRYGYSVERGRCVTFTYGGCPYNDNNFFTRAEC 243
            C Q  + GLC  Y  RY Y+  +GRC  F YGGC  N NNF T  EC
Sbjct:   25 CAQAPDSGLCFGYFERYFYNFRKGRCEIFIYGGCLGNQNNFLTEKEC 71          
BLAST of BPTI/Kunitz inhibitor domain-containing protein vs. Ensembl Medaka
Match: ENSORLT00000040309.1 (pep primary_assembly:ASM223467v1:11:20946373:20947959:-1 gene:ENSORLG00000024141.1 transcript:ENSORLT00000040309.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 49.2914 bits (116), Expect = 8.706e-9
Identity = 24/51 (47.06%), Postives = 29/51 (56.86%), Query Frame = 1
Query:  103 CNQPLEIGLCRQYRVRYGYSVERGRCVTFTYGGCPYNDNNFFTRAECEARC 255
            C+QPL+ G CR YRVR+ +  +   C  F YGGC  N NNF T   C   C
Sbjct:    8 CSQPLDPGPCRLYRVRWYFDPQANSCAQFWYGGCHGNQNNFETEEICRKAC 58          
BLAST of BPTI/Kunitz inhibitor domain-containing protein vs. Ensembl Medaka
Match: wfikkn2b (WAP, Kazal, immunoglobulin, Kunitz and NTR domain-containing protein 2-like [Source:NCBI gene;Acc:101161126])

HSP 1 Score: 52.7582 bits (125), Expect = 1.155e-8
Identity = 29/70 (41.43%), Postives = 36/70 (51.43%), Query Frame = 1
Query:   58 LFVTILSCIECKEPE----CNQPLEIGLCRQYRVRYGYSVERGRCVTFTYGGCPYNDNNFFTRAECEARC 255
            LF ++  C+ C  PE    C  P   G C+ Y  R+ YS     C TF YGGC  NDNNF ++  CE  C
Sbjct:  350 LFESLQDCMRCCGPEPSGPCGLPSLQGPCKAYEPRWAYSSALRGCHTFIYGGCGGNDNNFESKRACEDSC 419          
BLAST of BPTI/Kunitz inhibitor domain-containing protein vs. Ensembl Medaka
Match: ENSORLT00000012975.2 (pep primary_assembly:ASM223467v1:12:15740273:15760140:1 gene:ENSORLG00000010354.2 transcript:ENSORLT00000012975.2 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 51.2174 bits (121), Expect = 1.760e-8
Identity = 26/51 (50.98%), Postives = 29/51 (56.86%), Query Frame = 1
Query:  103 CNQPLEIGLCRQYRVRYGYSVERGRCVTFTYGGCPYNDNNFFTRAECEARC 255
            C Q  + GLC  Y  RY Y+  +GRC  F YGGC  N NNF T  EC  RC
Sbjct:  125 CAQAPDSGLCFGYFERYFYNFRKGRCEIFIYGGCLGNQNNFLTEKECLQRC 175          

HSP 2 Score: 48.521 bits (114), Expect = 1.431e-7
Identity = 25/50 (50.00%), Postives = 28/50 (56.00%), Query Frame = 1
Query:  103 CNQPLEIGLCRQYRVRYGYSVERGRCVTFTYGGCPYNDNNFFTRAECEAR 252
            C Q  + GLC  Y  RY Y+  +GRC  F YGGC  N NNF T  EC  R
Sbjct:   73 CAQAPDSGLCFGYFERYFYNFRKGRCEIFIYGGCLGNQNNFLTEKECLQR 122          
BLAST of BPTI/Kunitz inhibitor domain-containing protein vs. Ensembl Medaka
Match: ENSORLT00000044137.1 (pep primary_assembly:ASM223467v1:2:16334979:16376346:1 gene:ENSORLG00000025630.1 transcript:ENSORLT00000044137.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 50.8322 bits (120), Expect = 6.353e-8
Identity = 22/54 (40.74%), Postives = 28/54 (51.85%), Query Frame = 1
Query:   94 EPECNQPLEIGLCRQYRVRYGYSVERGRCVTFTYGGCPYNDNNFFTRAECEARC 255
            +P C+  L  G CR Y +R+ Y  +   C  F YGGC  N N F T  EC+  C
Sbjct:  955 DPRCSLVLVPGTCRDYNIRWYYDKQANACAQFWYGGCNGNGNRFDTEEECKRTC 1008          
BLAST of BPTI/Kunitz inhibitor domain-containing protein vs. Planmine SMEST
Match: SMESG000038005.1 (SMESG000038005.1)

HSP 1 Score: 147.132 bits (370), Expect = 7.287e-47
Identity = 72/81 (88.89%), Postives = 74/81 (91.36%), Query Frame = 1
Query:   37 MKSIIFLLFVTILSCIECKEPECNQPLEIGLCRQYRVRYGYSVERGRCVTFTYGGCPYNDNNFFTRAECEARCEN*F*KSF 279
            MKSIIFLLFVTILSCIECKEPECNQPLEIGLCRQYRVRYGYSVERGRCVTFTYGGCPYNDNNFFTRAECE   +  F K+ 
Sbjct:    1 MKSIIFLLFVTILSCIECKEPECNQPLEIGLCRQYRVRYGYSVERGRCVTFTYGGCPYNDNNFFTRAECEHDVKTNFKKAL 81          

HSP 2 Score: 49.6766 bits (117), Expect = 9.298e-9
Identity = 22/24 (91.67%), Postives = 23/24 (95.83%), Query Frame = 2
Query:  242 VKHDVKTNFKKALFYEIYNQTKLF 313
             +HDVKTNFKKALFYEIYNQTKLF
Sbjct:   69 CEHDVKTNFKKALFYEIYNQTKLF 92          
BLAST of BPTI/Kunitz inhibitor domain-containing protein vs. Planmine SMEST
Match: SMESG000038055.1 (SMESG000038055.1)

HSP 1 Score: 99.3673 bits (246), Expect = 2.974e-28
Identity = 50/76 (65.79%), Postives = 55/76 (72.37%), Query Frame = 1
Query:   37 MKSIIFL--LFVTILSCIECKEPECNQPLEIGLCRQYRVRYGYSVERGRCVTFTYGGCPYNDNNFFTRAECEARCE 258
            M S+I +  LFVT L  IE K PECN  LE GLCR Y VRYG+ V+ G+C TF YGGCP NDNNF TRAECE RCE
Sbjct:    1 MNSLIIISCLFVTALQLIEGKAPECNLSLETGLCRAYFVRYGFDVKLGKCRTFVYGGCPVNDNNFHTRAECEERCE 76          
BLAST of BPTI/Kunitz inhibitor domain-containing protein vs. Planmine SMEST
Match: SMESG000038052.1 (SMESG000038052.1)

HSP 1 Score: 93.5893 bits (231), Expect = 4.549e-26
Identity = 45/76 (59.21%), Postives = 52/76 (68.42%), Query Frame = 1
Query:   37 MKSIIFL--LFVTILSCIECKEPECNQPLEIGLCRQYRVRYGYSVERGRCVTFTYGGCPYNDNNFFTRAECEARCE 258
            M S++    +FV +L   E K PECN PLE GLC  Y VRYG+ V+ G+C TF YGGC  NDNNF TRAECE RCE
Sbjct:    1 MNSLVIFSCIFVAVLQLTESKSPECNLPLETGLCEAYFVRYGFDVKLGKCRTFVYGGCHVNDNNFHTRAECEERCE 76          
BLAST of BPTI/Kunitz inhibitor domain-containing protein vs. Planmine SMEST
Match: SMESG000013910.1 (SMESG000013910.1)

HSP 1 Score: 74.3294 bits (181), Expect = 1.456e-18
Identity = 35/69 (50.72%), Postives = 45/69 (65.22%), Query Frame = 1
Query:   55 LLFVTILSCIECKEPECNQPLEIGLCRQYRVRYGYSVERGRCVTFTYGGCPYNDNNFFTRAECEARCEN 261
            ++F   +   +CKEPEC+ P E+GLCR Y  RY ++ E G+C  F YGGC  N NNF+T  EC ARCE 
Sbjct:    4 VIFAVAIHLGDCKEPECSLPKEVGLCRGYFPRYFFNEETGKCEKFIYGGCQGNKNNFYTPIECRARCEQ 72          
BLAST of BPTI/Kunitz inhibitor domain-containing protein vs. Planmine SMEST
Match: SMESG000037991.1 (SMESG000037991.1)

HSP 1 Score: 71.633 bits (174), Expect = 1.923e-17
Identity = 31/59 (52.54%), Postives = 39/59 (66.10%), Query Frame = 1
Query:   85 ECKEPECNQPLEIGLCRQYRVRYGYSVERGRCVTFTYGGCPYNDNNFFTRAECEARCEN 261
            E + PECN  +E GLC  Y +RYG+  + G C+ F YGGCP N NNF T+ ECE  C+N
Sbjct:   19 ESRAPECNDTIETGLCDAYFIRYGFDTKLGECIQFIYGGCPVNKNNFKTKEECENLCKN 77          
The following BLAST results are available for this feature:
BLAST of BPTI/Kunitz inhibitor domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
EPPIN2.038e-835.29epididymal peptidase inhibitor [Source:HGNC Symbol... [more]
SPINT32.367e-842.31serine peptidase inhibitor, Kunitz type 3 [Source:... [more]
WFIKKN22.573e-838.71WAP, follistatin/kazal, immunoglobulin, kunitz and... [more]
WFIKKN22.918e-838.71WAP, follistatin/kazal, immunoglobulin, kunitz and... [more]
EPPIN3.219e-835.29epididymal peptidase inhibitor [Source:HGNC Symbol... [more]
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BLAST of BPTI/Kunitz inhibitor domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
mlt-111.456e-946.30pep chromosome:WBcel235:V:20667209:20678513:1 gene... [more]
mlt-111.456e-946.30pep chromosome:WBcel235:V:20667209:20678513:1 gene... [more]
mlt-111.502e-946.30pep chromosome:WBcel235:V:20667209:20678513:1 gene... [more]
mlt-111.503e-946.30pep chromosome:WBcel235:V:20667211:20678513:1 gene... [more]
mlt-111.538e-946.30pep chromosome:WBcel235:V:20667211:20678513:1 gene... [more]
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BLAST of BPTI/Kunitz inhibitor domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Ppn1.417e-946.15gene:FBgn0003137 transcript:FBtr0301838[more]
Ppn1.562e-946.15gene:FBgn0003137 transcript:FBtr0301839[more]
Ppn1.562e-946.15gene:FBgn0003137 transcript:FBtr0301837[more]
CG154185.727e-945.10gene:FBgn0031554 transcript:FBtr0077556[more]
CG154185.727e-945.10gene:FBgn0031554 transcript:FBtr0446100[more]
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BLAST of BPTI/Kunitz inhibitor domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
col28a2a8.728e-940.74collagen, type XXVIII, alpha 2a [Source:ZFIN;Acc:Z... [more]
col28a2a8.992e-940.74collagen, type XXVIII, alpha 2a [Source:ZFIN;Acc:Z... [more]
col6a4a3.353e-841.18collagen, type VI, alpha 4a [Source:ZFIN;Acc:ZDB-G... [more]
si:ch211-62a1.38.912e-843.18si:ch211-62a1.3 [Source:ZFIN;Acc:ZDB-GENE-061207-4... [more]
spint1b1.143e-734.85serine peptidase inhibitor, Kunitz type 1 b [Sourc... [more]
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BLAST of BPTI/Kunitz inhibitor domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
WFIKKN23.093e-940.32WAP, follistatin/kazal, immunoglobulin, kunitz and... [more]
cmtr29.304e-939.58cap methyltransferase 2 [Source:Xenbase;Acc:XB-GEN... [more]
cmtr21.296e-837.25cap methyltransferase 2 [Source:Xenbase;Acc:XB-GEN... [more]
itch3.423e-844.44itchy E3 ubiquitin protein ligase [Source:Xenbase;... [more]
itch3.428e-844.44itchy E3 ubiquitin protein ligase [Source:Xenbase;... [more]
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BLAST of BPTI/Kunitz inhibitor domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Spint31.213e-1140.74serine peptidase inhibitor, Kunitz type, 3 [Source... [more]
Wfdc6b4.628e-836.76WAP four-disulfide core domain 6B [Source:MGI Symb... [more]
Wfikkn21.273e-738.71WAP, follistatin/kazal, immunoglobulin, kunitz and... [more]
Aplp28.369e-740.38amyloid beta (A4) precursor-like protein 2 [Source... [more]
Ambp1.014e-637.25alpha 1 microglobulin/bikunin precursor [Source:MG... [more]
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BLAST of BPTI/Kunitz inhibitor domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|D8KY58|VKT_BOMTE1.104e-1050.94Kunitz-type serine protease inhibitor Bt-KTI OS=Bo... [more]
sp|P0DJ50|VKT31_MESMA1.014e-930.56Kunitz-type serine protease inhibitor BmKTT-2 OS=M... [more]
sp|P0DMJ3|VKTI1_ANTFU1.078e-946.15PI-actitoxin-Afv2a OS=Anthopleura fuscoviridis OX=... [more]
sp|Q589G4|VKT19_ANOSM1.334e-938.89Kunitz-type serine protease inhibitor As-fr-19 OS=... [more]
sp|Q8T0W4|VKT2_PIMHY2.689e-938.57Kunitz-type serine protease inhibitor cvp2 OS=Pimp... [more]
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BLAST of BPTI/Kunitz inhibitor domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A1I8NL514.574e-1348.44BPTI/Kunitz inhibitor domain-containing protein OS... [more]
A0A0R3WAZ96.470e-1257.14Uncharacterized protein OS=Taenia asiatica OX=6051... [more]
A0A0R3WBP82.415e-1141.10BPTI/Kunitz inhibitor domain-containing protein OS... [more]
A0A2L2Y5722.530e-1140.26Microlepidin-1 (Fragment) OS=Parasteatoda tepidari... [more]
A0A3P8DGB23.745e-1142.86BPTI/Kunitz inhibitor domain-containing protein OS... [more]
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BLAST of BPTI/Kunitz inhibitor domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
col28a2a3.176e-942.59collagen alpha-1(XXVIII) chain-like [Source:NCBI g... [more]
wfikkn2b1.219e-843.55WAP, Kazal, immunoglobulin, Kunitz and NTR domain-... [more]
col28a2b1.302e-840.74collagen, type XXVIII, alpha 2b [Source:ZFIN;Acc:Z... [more]
ENSAMXT00000056342.12.693e-833.85pep primary_assembly:Astyanax_mexicanus-2.0:25:410... [more]
wfikkn13.882e-843.14WAP, follistatin/kazal, immunoglobulin, kunitz and... [more]
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BLAST of BPTI/Kunitz inhibitor domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5
Match NameE-valueIdentityDescription
WFIKKN12.184e-836.07WAP, follistatin/kazal, immunoglobulin, kunitz and... [more]
ENSPMAT00000008261.16.014e-736.67pep scaffold:Pmarinus_7.0:GL478901:735:1448:1 gene... [more]
tfpi26.152e-733.77tissue factor pathway inhibitor 2 [Source:ZFIN;Acc... [more]
ENSPMAT00000005043.15.554e-646.15pep scaffold:Pmarinus_7.0:GL481015:31246:35903:1 g... [more]
ENSPMAT00000005038.16.147e-646.15pep scaffold:Pmarinus_7.0:GL481015:31246:35903:1 g... [more]
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BLAST of BPTI/Kunitz inhibitor domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of BPTI/Kunitz inhibitor domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO416969.965e-1048.08Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO262639.965e-1048.08Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO317591.115e-843.14Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO298199.229e-841.18Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO382849.822e-843.14Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of BPTI/Kunitz inhibitor domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSORLT00000042019.17.298e-948.28pep primary_assembly:ASM223467v1:12:15740119:15780... [more]
ENSORLT00000040309.18.706e-947.06pep primary_assembly:ASM223467v1:11:20946373:20947... [more]
wfikkn2b1.155e-841.43WAP, Kazal, immunoglobulin, Kunitz and NTR domain-... [more]
ENSORLT00000012975.21.760e-850.98pep primary_assembly:ASM223467v1:12:15740273:15760... [more]
ENSORLT00000044137.16.353e-840.74pep primary_assembly:ASM223467v1:2:16334979:163763... [more]
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BLAST of BPTI/Kunitz inhibitor domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000038005.17.287e-4788.89SMESG000038005.1[more]
SMESG000038055.12.974e-2865.79SMESG000038055.1[more]
SMESG000038052.14.549e-2659.21SMESG000038052.1[more]
SMESG000013910.11.456e-1850.72SMESG000013910.1[more]
SMESG000037991.11.923e-1752.54SMESG000037991.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30023241 ID=SMED30023241|Name=BPTI/Kunitz inhibitor domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=409bp
AAACTGGTGATAAAATATTTTTTTAGAATATAAACTATGAAGTCTATTAT
ATTTTTATTATTTGTTACCATCCTAAGTTGCATTGAATGCAAAGAGCCGG
AATGTAATCAGCCCCTTGAAATTGGTTTATGCCGACAATATCGAGTAAGA
TACGGATATAGCGTCGAAAGAGGAAGATGTGTTACATTTACTTACGGAGG
TTGTCCATATAATGATAATAACTTCTTCACAAGAGCCGAATGTGAAGCAC
GATGTGAAAACTAATTTTAAAAAAGCTTTATTTTATGAAATATATAATCA
AACTAAATTGTTTTAATATTTAGAAATATATTTTTCAAAATTTAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAN
AAAAAAANA
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000067goblet cell
PLANA:0000419prepharyngeal region
PLANA:0000421tail
PLANA:0002075pigment cell
Vocabulary: INTERPRO
TermDefinition
IPR036880Kunitz_BPTI_sf
IPR002223Kunitz_BPTI
IPR020901Prtase_inh_Kunz-CS
Vocabulary: molecular function
TermDefinition
GO:0004867serine-type endopeptidase inhibitor activity
GO:0008233peptidase activity
Vocabulary: biological process
TermDefinition
GO:0006508proteolysis
GO:0010951negative regulation of endopeptidase activity
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002223Pancreatic trypsin inhibitor Kunitz domainSMARTSM00131KU_2coord: 21..74
e-value: 4.7E-10
score: 49.4
IPR002223Pancreatic trypsin inhibitor Kunitz domainPFAMPF00014Kunitz_BPTIcoord: 23..71
e-value: 2.3E-13
score: 50.1
IPR002223Pancreatic trypsin inhibitor Kunitz domainPROSITEPS50279BPTI_KUNITZ_2coord: 23..73
score: 11.891
IPR002223Pancreatic trypsin inhibitor Kunitz domainCDDcd00109KUcoord: 23..71
e-value: 1.40615E-14
score: 59.1858
IPR036880Pancreatic trypsin inhibitor Kunitz domain superfamilyGENE3DG3DSA:4.10.410.10coord: 20..75
e-value: 5.0E-16
score: 60.4
IPR036880Pancreatic trypsin inhibitor Kunitz domain superfamilySUPERFAMILYSSF57362BPTI-likecoord: 13..71
NoneNo IPR availablePANTHERPTHR45938FAMILY NOT NAMEDcoord: 13..71
NoneNo IPR availablePANTHERPTHR45938:SF9ACP24A4-RELATEDcoord: 13..71
NoneNo IPR availablePROSITEPS51257PROKAR_LIPOPROTEINcoord: 1..15
score: 5.0
NoneNo IPR availableSIGNALP_EUKSignalP-noTMSignalP-noTMcoord: 1..18
score: 0.834
IPR020901Proteinase inhibitor I2, Kunitz, conserved sitePROSITEPS00280BPTI_KUNITZ_1coord: 51..69