SMED30023228

Overview
NameSMED30023228
Smed IDSMED30023228
Length (bp)3240
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30023228 (SMED30023228) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30023228 (SMED30023228) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30023228 (SMED30023228) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30023228 (SMED30023228) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30023228

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 12

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30023228SMESG000016978.1 SmedASXL_007176SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
protonephridiaSMED30023228SMESG000016978.1 dd_Smed_v4_19646_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30023228SMESG000016978.1 dd_Smed_v4_19646_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymaSMED30023228SMESG000016978.1 dd_Smed_v4_19646_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
pharynxSMED30023228SMESG000058720.1 dd_Smed_v4_7931_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30023228SMESG000058720.1 dd_Smed_v4_7931_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30023228SMESG000058720.1 dd_Smed_v4_7931_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30023228SMESG000058720.1 dd_Smed_v4_7931_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30023228SMESG000058720.1 dd_Smed_v4_7931_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neuronSMED30023228SMESG000058720.1 dd_Smed_v4_7931_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		whole organism asexual adult colorimetric in situ hybridization evidence
head regionSMED30023228SMESG000058720.1 dd_Smed_v6_7931_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
epidermisSMED30023228SMESG000058720.1 dd_Smed_v4_7931_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
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BLAST of SMED30023228 vs. Planmine SMEST
Match: SMESG000058720.1 (SMESG000058720.1)

HSP 1 Score: 574.318 bits (1479), Expect = 0.000e+0
Identity = 311/347 (89.63%), Postives = 314/347 (90.49%), Query Frame = 2 
Query:    2 KELINKIGKYERECSECVRLQRKCENCTRKRKANGYDSDEGSSIRNPEIPKKNNLQIETDESNNSVSLDENSCSASENGELISKGLQSSIDIKRRKTTWKCEFCSSTNENNLENCRNCYKVRKNIEELEKKPNIGKELIN----------------------TRKRNANIQIRPEKNPPVPQRNNPSCSNGNKKIEKVSNDDNNYNQWICVGCSKLQRKCEVCSGKIKPNKEDNIQSSPKRKSPIIYRENPRNYSQIPNENNQSKNLLQTRGDNQKQPIVKQSPPIGQTGRSRSPQKLINENKKTNEIYGKCKKCKREKELIHHKKCKSCQSLSYRIKMFLICGA*A 976
            KE INKIGKYERECSECVRLQRKCENCTRKRKANGYDSDEGSSIRNPEIPKKNNLQIETDESNNSVSL+ENSCSASENGELISKG QSSIDIKRRKTTWKCEFCSSTNENNLENCRNCYKVR NIEE EKKPNIGKE IN                      TRKRNANIQIRPEKN PVPQRNNPSCSNGNKKIEKVSNDDNNYNQ ICVGCSKLQRKCEVCSGK  PNKEDNIQ+SPKRKSPIIYRENPRNYSQIPNENNQSKNLLQTRGDNQKQPIVKQSPPIGQTGRSRSPQKLINENKKTNEIYGKCKKCKREKELI+HKKCKSCQSL YRIKMFLICGA A
Sbjct:  337 KESINKIGKYERECSECVRLQRKCENCTRKRKANGYDSDEGSSIRNPEIPKKNNLQIETDESNNSVSLEENSCSASENGELISKGFQSSIDIKRRKTTWKCEFCSSTNENNLENCRNCYKVRTNIEESEKKPNIGKESINKIGKYERECSECVRLQRKCENCTRKRNANIQIRPEKNHPVPQRNNPSCSNGNKKIEKVSNDDNNYNQLICVGCSKLQRKCEVCSGKRIPNKEDNIQNSPKRKSPIIYRENPRNYSQIPNENNQSKNLLQTRGDNQKQPIVKQSPPIGQTGRSRSPQKLINENKKTNEIYGKCKKCKREKELIYHKKCKSCQSLRYRIKMFLICGASA 683

HSP 2 Score: 150.984 bits (380), Expect = 2.250e-37
Identity = 96/169 (56.80%), Postives = 102/169 (60.36%), Query Frame = 2 
Query:  164 LQIETDESNNSVSLDENSCSASENGELISKGLQSSIDIKRRKTTWKCEFCSSTNENNLENCRNCYKVRKNIEELEKKPNIGKELINTRKRNANIQIRPEKNPPVPQRNNPSCSNGNKKIEKVSNDDNNYNQWICVGCSKLQRKCEVCSGKIKPNKEDNIQSSPKRKSPI 670
            LQ ETDES    S+DENSCSASENGELISKGLQSSIDIKRRKTTWKCEFCSSTNENNLENCRNCYKVR NIEE EKKPNIGKE IN                               KI K   +        C  C +LQRKCE C+ K K N  D+ + S  R   I
Sbjct:  260 LQFETDES----SMDENSCSASENGELISKGLQSSIDIKRRKTTWKCEFCSSTNENNLENCRNCYKVRTNIEESEKKPNIGKESIN-------------------------------KIGKYERE--------CSECVRLQRKCENCTRKRKANGYDSDEGSSIRNPEI 385
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BLAST of SMED30023228 vs. Planmine SMEST
Match: SMESG000016978.1 (SMESG000016978.1)

HSP 1 Score: 360.147 bits (923), Expect = 3.001e-114
Identity = 176/181 (97.24%), Postives = 177/181 (97.79%), Query Frame = -2 
Query: 1914 FLQYLLSTINGQYYRITDNSCVQGICTIYSYNFQRNISGLFLTGIERRPCFRLNCTNCFLKGEWNITYFLTSGILTRDIQYCKADAKNDLLNLFPNSVSTIYGIQCLNYSCTVPVYKTYNSILYVTELIKCNSQSCWNCHTSRFWQQNVTQSKIIERYIHFCNESKTIDVRLAFFNYSQGK 2456
            F  +LLSTINGQYYRITDNSCVQGICTIYSYN QRNISGLFLTGIERRPCFRLNCTNCFLKGEWNITYFLTSGILTRDIQYCKADAKNDLLNLFPNSVSTIYGIQCLNYSCTVPVYKTYNSILYVTELIKCNSQSCWNCHTSRFWQQNVTQSKIIERYIHFCNESKTIDVRLAFFNYSQ K
Sbjct:    6 FAIFLLSTINGQYYRITDNSCVQGICTIYSYNLQRNISGLFLTGIERRPCFRLNCTNCFLKGEWNITYFLTSGILTRDIQYCKADAKNDLLNLFPNSVSTIYGIQCLNYSCTVPVYKTYNSILYVTELIKCNSQSCWNCHTSRFWQQNVTQSKIIERYIHFCNESKTIDVRLAFFNYSQDK 186

HSP 2 Score: 57.3806 bits (137), Expect = 3.726e-8
Identity = 43/160 (26.88%), Postives = 67/160 (41.88%), Query Frame = -2 
Query: 1935 DNSCVQGICTIYSYNFQRNISGLFLTGIERRPCFRLNCTNCFLKGEWNITYFLTSGILTRDIQYCKADAKNDLLNLFPNSVSTIYGIQCLNYSCTVPVYKTYNSILYVTELIKCN-SQSCWNCHTSRFWQQN--VTQSKIIERYIHFCNESKTIDVRLAF 2405
            D SC  G C I+ Y    +     L    +  C  L+C NC  KGEW +        L R+  +C  +    +  +FP S ST+  I+C +Y C    Y+   S    T + +C  S  C NC     W +   +     ++R  + C E + I++   F
Sbjct:  194 DISCKYGQCQIFRYQLYFDGVSYSLRKDFQSTCQSLSCYNCIYKGEWTLMESNGHYFLQRNTSFCSMEPTRFINEVFPESYSTVTDIECQHYRCKKTFYQQPPSYQLKTYMEECQMSTICQNCRYVSSWNETNLIRIGFFLKRESYLCPEIEYINIADYF 353
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BLAST of SMED30023228 vs. Planmine SMEST
Match: SMESG000017154.1 (SMESG000017154.1)

HSP 1 Score: 76.6406 bits (187), Expect = 3.791e-15
Identity = 46/137 (33.58%), Postives = 66/137 (48.18%), Query Frame = -2 
Query: 2001 TDNSCVQGICTIYSYNFQRNISGLFLTGIERRPCFRLNCTNCFLKGEWNITYFLTSGILTRDIQYCKADAKNDLLNLFPN-SVSTIYGIQCLNYSCTVPVYKTYNSILYVTELIKCNSQSCWNCHTSRFWQQNVTQS 2408
            TD  C  G C    Y   ++ SG  +  + +  C  +NC NC  KG+W +     +  LTR+   C AD+  +   +FP+ S S +  I C +Y CT+  Y      LY   L KC+  SC  C+   +WQ N TQS
Sbjct:   24 TDIRCANGACYNVVYELYQDSSGYRMNVVTKSDCPPINCNNCVYKGDWTLIASSGAYYLTRNTFLCLADSFRNFTEIFPDPSSSNMVDIACNSYQCTIYHYIIKEPFLYTYNLGKCSDFSCEGCNMCNYWQLNKTQS 160
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BLAST of SMED30023228 vs. Planmine SMEST
Match: SMESG000017127.1 (SMESG000017127.1)

HSP 1 Score: 59.3066 bits (142), Expect = 2.322e-9
Identity = 55/180 (30.56%), Postives = 82/180 (45.56%), Query Frame = -2 
Query: 1926 FLQYLLST--INGQYYRITDNSCVQGICTIYSYNFQRNISGLFLTGIERRPCFRLNCTNCFLKGEWNITYFLTSGILTRDIQYCKADAKNDLLNLFPNSVSTIYGIQCLNYSCTVPVYKTYNSILYVTE-LIKCNSQSCWNCHTSRFWQQNVTQSKIIERYIHFCNESKTIDVRLAFFNY 2456
             LQYL+ +  IN +Y    D SC  G C + SY  +     L ++  +   C  L+C NC +K +WNI     SGIL+R +  C  + +  +  +F  +  T+  IQC N  C+   Y +Y    Y T  L  C    C +C  S  W  N   +  +ER ++ C  +  I     FF Y
Sbjct:   11 LLQYLIGSKIINKRY----DFSCNYGTCRLVSYQLESIYGRLTVSSSQVGSCSSLSCANCTVKSDWNI----FSGILSRTVVNCLTEQRKFVSEIFTYAAYTMADIQCFNNKCSKYSYSSY--FPYETNYLGSCQDWQCSDCLRSMIWTYNA--NGYLERDVYSCPAASKITAE-DFFIY 177
[Close]
BLAST of SMED30023228 vs. Planmine SMEST
Match: SMESG000057635.1 (SMESG000057635.1)

HSP 1 Score: 58.5362 bits (140), Expect = 4.964e-9
Identity = 41/162 (25.31%), Postives = 71/162 (43.83%), Query Frame = -2 
Query: 1935 DNSCVQGICTIYSYNFQRNISGLFLTGIERRPCFRLNCTNCFLKGEWNITYFLTSGILTRDIQYCKADAKNDLLNLFPNSVSTIYGIQCLNYSCTVPVYKT-YNSILY--VTELIKCNSQSCWNCHTSRFWQQNVTQSK--IIERYIHFCNESKTIDVRLAF 2405
            D +C+ G C++  Y          L      PC  L+C+ C +KG W+++       L+R  + C  + +  L  +FP   S +  IQC  ++C   +Y+  +N I++  +T    CN  +C +C     W  N T  +   I R  ++C     I +   F
Sbjct:   26 DVTCLNGKCSVIRYKLYFQNQTYSLRSAREFPCVSLSCSGCAVKGHWSLSEISGKYFLSRFTKNCSIENEKHLSEMFPFYDSKVSEIQCYGFTCRKTIYRMQHNKIIFYSLTGNYSCNKIACRSCSYLGRWVLNNTLGRNLTITRSTNYCAAPNMIAMEDYF 187
The following BLAST results are available for this feature:
BLAST of SMED30023228 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023228 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023228 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023228 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023228 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023228 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023228 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023228 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023228 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023228 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023228 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023228 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023228 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023228 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000058720.12.250e-3789.63SMESG000058720.1[more]
SMESG000016978.13.001e-11497.24SMESG000016978.1[more]
SMESG000017154.13.791e-1533.58SMESG000017154.1[more]
SMESG000017127.12.322e-930.56SMESG000017127.1[more]
SMESG000057635.14.964e-925.31SMESG000057635.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30023228 ID=SMED30023228|Name=SMED30023228|organism=Schmidtea mediterranea sexual|type=transcript|length=3240bp
GAAGGAATTGATTAATAAGATAGGAAAATATGAAAGGGAATGCAGTGAAT
GCGTTAGACTTCAAAGAAAATGTGAAAATTGTACTCGAAAAAGAAAAGCC
AATGGATATGATAGTGATGAAGGTTCATCGATAAGAAATCCAGAGATTCC
TAAAAAGAATAATTTACAAATCGAAACAGATGAGTCTAATAATTCCGTTT
CTTTGGACGAGAATAGTTGTTCAGCAAGTGAGAATGGAGAATTGATTTCC
AAAGGTTTACAGTCTTCTATTGATATTAAGAGGAGGAAAACTACTTGGAA
ATGCGAATTTTGCAGTTCTACAAATGAAAATAATTTAGAAAATTGTAGAA
ATTGTTATAAAGTAAGGAAAAACATCGAAGAATTGGAAAAAAAGCCTAAT
ATTGGCAAGGAATTGATTAATACTAGAAAAAGAAACGCCAATATTCAAAT
TAGACCAGAAAAAAATCCTCCTGTTCCTCAGAGAAATAATCCCTCTTGCA
GTAATGGAAATAAAAAGATAGAAAAAGTGTCAAATGATGATAATAATTAT
AATCAATGGATTTGTGTTGGATGTTCAAAACTTCAAAGAAAATGTGAAGT
TTGTTCTGGGAAAATAAAACCCAATAAAGAAGATAATATACAAAGTTCAC
CGAAAAGAAAATCACCAATAATTTACCGGGAAAATCCAAGAAATTATTCA
CAGATTCCCAATGAGAATAATCAATCGAAAAATCTATTGCAAACTAGAGG
TGACAATCAAAAACAACCAATTGTTAAACAAAGTCCCCCCATTGGACAGA
CTGGCCGATCGCGTTCACCACAGAAACTGATTAACGAAAACAAAAAGACA
AATGAGATTTATGGTAAATGTAAGAAATGTAAACGTGAAAAAGAACTAAT
TCACCATAAGAAATGTAAATCGTGTCAATCATTGAGTTACAGAATCAAAA
TGTTTCTTATTTGTGGAGCTTGAGCATAATATTTTCGAAAAATGTTTCTC
GACTTTAAATATCGAAACTTCTCATTGAATTTTTTCGATTCTTTTTTCAT
CGATAATGGAATTCATTTTCTATTTACGTCGGTGTAAATTTTTCTTCGAA
TTAGTTTCTCTGTGATAATAATAATTTTATGCACTATTATGAAGATTGCT
ATTCGATATCATTGGTTAGATTGATTTTGAGTCTTCTTTGATCCATTACT
TTTTATTAGTATTTTTAGAATGTATTAATTTAATTTACTCTAAATTTTCA
TATTTAACATTTTATATTCGATTTCTTATTAAAATCTCAGCTAATAGTTG
ATTTACCCCGTAAAATCAAATATCACAATTTACCCCCTTAAAAAATCATA
AATCTGATTTTATCACCGATATCCAACCAATGGGATCTCTTAATTTTCAT
AAAAGCTACCAACTTGAAATCATTATTATAAGAAAGAAACAATTTTGCCT
GTCTGTGTAGGAAACGGTTAGGTAACGGGAAGAGATTAATTTTTCAAAGA
CTAAGCACTAATTTAATAGCTGCAGAAATCAATCAAAGATAACATGTTTA
AATACATTAAATAGACGTTTTATAGAGATGCCAATTTAAAAAATGACTAA
TAATAACGAACAAGGCCAAACTTTCAATTTTTATATTGTTGGGTTAATAT
CGTTTAAAAGTCGGTTCTCGCTGACTCCTCGTGTTCTTTATATAGAAATG
AAGAAAACCCAAACCCAACCCAATTTAGCATATATATATTATTCAAATAA
GTACAAAATGTGTAGGAGGGCATAATACTCTTTCGCTACCTTACTGTGTT
AAGAAGATGAAAAAGAAAAACTGGGACTTGCATTTGTACTCTCCAATAAT
AATGCAGGTTCAACAGAGATCCCCAAGTCTTTTAAAATAATTCAAAAATA
TAAGAAATGATCATTTACCTTGTGAATAGTTGAAAAAGGCTAGCCGAACA
TCAATCGTTTTACTCTCATTGCAAAAATGAATGTATCTTTCAATAATTTT
ACTTTGTGTAACGTTCTGCTGCCAGAATCTTGAAGTATGGCAATTCCAAC
ATGACTGGCTATTACACTTTATCAGTTCTGTCACATAGAGAATCGAGTTA
TATGTTTTATAAACGGGCACAGTGCAACTGTAATTGAGACACTGAATTCC
ATATATTGTTGAAACTGAGTTGGGAAATAAATTGAGCAAGTCATTTTTAG
CGTCTGCTTTACAGTATTGAATATCTCGCGTTAGAATCCCACTTGTCAAA
AAGTAAGTAATGTTCCATTCGCCTTTCAGAAAGCAATTAGTGCAGTTCAA
TCGAAAACAAGGTCTTCTCTCAATTCCTGTTAAGAATAAACCAGATATAT
TTCTCTGGAAATTGTAGCTATAAATGGTGCAAATCCCTTGGACACAAGAG
TTATCTGTGATCCTGTAATATTGCCCATTGATTGTGGACAGAAGATATTG
CAAAAAGTTTAGAAATCATCTTGCACAATTAATTTCAACAACTCTGACAT
TTTATTTTGTATGAAAACTGAAAGTTTACGTTCTCGGTTTTGTTTAATAG
AAACATTGGCGTTTCTGAAACTTAATCTAATTCAATGCAAATTTATAGAC
TTGTAATAGAATCGCAGTTTGTGGCGTTTGGAATGTTTAAGATTGGTTCA
CAATGGATGATTAAAAATTAATTTTCATGTCAAAATATTTGTAAAATTGA
ATTGGTTTTTTAAAATATTTGATAGTTTTGCTAGGGGACTCTTGGGAACC
CTTTATTACAATCTCATCTAAGCTGAAATAAAATTGAAATATAAGCAAAA
TAGTTAGAAATCCACAAAAAGAAATTTTCCCTCTTTTTATGAGAGAGCCC
AACGAACAAGATAGCATTTCAAAAAACAGTACCTAAAACATTGATCAACA
GGAAAATAGATGAAATAATTAAGATATAATCAGCCATTATAAATTAATGA
AGCGCTTTTTATTTATAAGAGTCAGCACCGTACCATGGCAAGTGCAATCG
CACTGTATCAAAGGGCCCCCAAATTCGCAAATTGTTTCCTTCTATCTGGT
TATAGGGTTGTCAGGCGTCCCGGATTTTATGTTTTTTGGTGTCCCGAAAA
AAAGTTTCAGGACGCTCAATTGTCTCAAGTTTTCAGAAAAAAAAATTATT
GAAAAAACTTGTGTCTCTTCGTTCACTTACGGTTTATTTTTTCCAATTCT
CGATGCAGCCTATATATTTTTACTGAGTTTTCATTACGGT
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protein sequence of SMED30023228-orf-1

>SMED30023228-orf-1 ID=SMED30023228-orf-1|Name=SMED30023228-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=324bp
KELINKIGKYERECSECVRLQRKCENCTRKRKANGYDSDEGSSIRNPEIP
KKNNLQIETDESNNSVSLDENSCSASENGELISKGLQSSIDIKRRKTTWK
CEFCSSTNENNLENCRNCYKVRKNIEELEKKPNIGKELINTRKRNANIQI
RPEKNPPVPQRNNPSCSNGNKKIEKVSNDDNNYNQWICVGCSKLQRKCEV
CSGKIKPNKEDNIQSSPKRKSPIIYRENPRNYSQIPNENNQSKNLLQTRG
DNQKQPIVKQSPPIGQTGRSRSPQKLINENKKTNEIYGKCKKCKREKELI
HHKKCKSCQSLSYRIKMFLICGA*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000020protonephridia
PLANA:0000034epidermis
PLANA:0000099neuron
PLANA:0000418head
PLANA:0002032epidermal cell
Vocabulary: INTERPRO
TermDefinition
IPR001876Znf_RanBP2
Vocabulary: molecular function
TermDefinition
GO:0008270zinc ion binding