SMED30023201

Overview
NameSMED30023201
Smed IDSMED30023201
Length (bp)1164
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30023201 (SMED30023201) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30023201 (SMED30023201) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30023201 (SMED30023201) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30023201 (SMED30023201) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30023201

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 15

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
Smed sexual biotypeSMED30023201h1SMcG0015585 Contig43499newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30023201h1SMcG0015585 Contig43499uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
nervous systemSMED30023201h1SMcG0015585 dd_Smed_v4_1903_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30023201h1SMcG0015585 dd_Smed_v4_1903_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30023201h1SMcG0015585 dd_Smed_v4_1903_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30023201h1SMcG0015585 dd_Smed_v4_1903_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30023201h1SMcG0015585 dd_Smed_v4_1903_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30023201h1SMcG0015585 dd_Smed_v4_1903_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cilated neuronSMED30023201h1SMcG0015585 dd_Smed_v4_1903_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30023201h1SMcG0015585 Contig36975newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
central nervous systemSMED30023201h1SMcG0015585 DN300628smed_ncbi_20240424PMID:16344473
Zayas et al., 2005
whole organism adult hermaphrodite colorimetric in situ hybridization evidence
testisSMED30023201h1SMcG0015585 DN300628smed_ncbi_20240424PMID:16344473
Zayas et al., 2005
whole organism adult hermaphrodite colorimetric in situ hybridization evidence
pigment cellSMED30023201h1SMcG0015585 dd_Smed_v6_1903_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30023201h1SMcG0015585 dd_Smed_v6_1903_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30023201h1SMcG0015585 dd_Smed_v4_1903_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017
whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30023201 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.003231:5905..12766 -5591
Homology
BLAST of SMED30023201 vs. Ensembl Human
Match: TPM1 (tropomyosin 1 [Source:HGNC Symbol;Acc:HGNC:12010])

HSP 1 Score: 78.5666 bits (192), Expect = 4.886e-16
Identity = 66/210 (31.43%), Postives = 130/210 (61.90%), Query Frame = 3
Query:   72 SGNSAVNALKSKMQNLKTEIDELKSKIEEKDKLLQEEI-HKKS---SIDQEIETLNKKIRELESQTDELDEKLSLSSIELDEVSKRADINERTRRGLESRKQNNDSKICKLESQLQEXXXXXXXXXXXYDEISKALKDSEMKLAEAEDRSEAATKRLKETDSEMHGVSHTLKSMELNESKATLREENYEKQINKITLQLKDAQTRYEIQE 689
            +G+S++ A++ K+++L+ + D      EE+   LQ E+ H++    + + ++ +LN++I+ +E + D   E+L+ +  +L+E  K AD +ER  + +ESR Q ++ K+   E QL+EA ++   A++KY+E+++ L   E  L  AE+R+E +  +  E + E+  V++ LKS+E    K + +E+ YE++I  ++ +LK+A+TR E  E
Sbjct:    2 AGSSSLEAVRRKIRSLQEQAD----AAEERAGTLQRELDHERKLRETAEADVASLNRRIQLVEEELDRAQERLATALQKLEEAEKAADESERGMKVIESRAQKDEEKMEIQEIQLKEAKHIAEDADRKYEEVARKLVIIESDLERAEERAELSEGKCAELEEELKTVTNNLKSLEAQAEKYSQKEDRYEEEIKVLSDKLKEAETRAEFAE 207          
BLAST of SMED30023201 vs. Ensembl Human
Match: TPM1 (tropomyosin 1 [Source:HGNC Symbol;Acc:HGNC:12010])

HSP 1 Score: 78.5666 bits (192), Expect = 5.324e-16
Identity = 66/210 (31.43%), Postives = 130/210 (61.90%), Query Frame = 3
Query:   72 SGNSAVNALKSKMQNLKTEIDELKSKIEEKDKLLQEEI-HKKS---SIDQEIETLNKKIRELESQTDELDEKLSLSSIELDEVSKRADINERTRRGLESRKQNNDSKICKLESQLQEXXXXXXXXXXXYDEISKALKDSEMKLAEAEDRSEAATKRLKETDSEMHGVSHTLKSMELNESKATLREENYEKQINKITLQLKDAQTRYEIQE 689
            +G+S++ A++ K+++L+ + D      EE+   LQ E+ H++    + + ++ +LN++I+ +E + D   E+L+ +  +L+E  K AD +ER  + +ESR Q ++ K+   E QL+EA ++   A++KY+E+++ L   E  L  AE+R+E +  +  E + E+  V++ LKS+E    K + +E+ YE++I  ++ +LK+A+TR E  E
Sbjct:    2 AGSSSLEAVRRKIRSLQEQAD----AAEERAGTLQRELDHERKLRETAEADVASLNRRIQLVEEELDRAQERLATALQKLEEAEKAADESERGMKVIESRAQKDEEKMEIQEIQLKEAKHIAEDADRKYEEVARKLVIIESDLERAEERAELSEGKCAELEEELKTVTNNLKSLEAQAEKYSQKEDRYEEEIKVLSDKLKEAETRAEFAE 207          
BLAST of SMED30023201 vs. Ensembl Human
Match: TPM1 (tropomyosin 1 [Source:HGNC Symbol;Acc:HGNC:12010])

HSP 1 Score: 78.5666 bits (192), Expect = 5.330e-16
Identity = 66/210 (31.43%), Postives = 130/210 (61.90%), Query Frame = 3
Query:   72 SGNSAVNALKSKMQNLKTEIDELKSKIEEKDKLLQEEI-HKKS---SIDQEIETLNKKIRELESQTDELDEKLSLSSIELDEVSKRADINERTRRGLESRKQNNDSKICKLESQLQEXXXXXXXXXXXYDEISKALKDSEMKLAEAEDRSEAATKRLKETDSEMHGVSHTLKSMELNESKATLREENYEKQINKITLQLKDAQTRYEIQE 689
            +G+S++ A++ K+++L+ + D      EE+   LQ E+ H++    + + ++ +LN++I+ +E + D   E+L+ +  +L+E  K AD +ER  + +ESR Q ++ K+   E QL+EA ++   A++KY+E+++ L   E  L  AE+R+E +  +  E + E+  V++ LKS+E    K + +E+ YE++I  ++ +LK+A+TR E  E
Sbjct:    2 AGSSSLEAVRRKIRSLQEQAD----AAEERAGTLQRELDHERKLRETAEADVASLNRRIQLVEEELDRAQERLATALQKLEEAEKAADESERGMKVIESRAQKDEEKMEIQEIQLKEAKHIAEDADRKYEEVARKLVIIESDLERAEERAELSEGKCAELEEELKTVTNNLKSLEAQAEKYSQKEDRYEEEIKVLSDKLKEAETRAEFAE 207          
BLAST of SMED30023201 vs. Ensembl Human
Match: TPM1 (tropomyosin 1 [Source:HGNC Symbol;Acc:HGNC:12010])

HSP 1 Score: 78.1814 bits (191), Expect = 6.531e-16
Identity = 66/210 (31.43%), Postives = 130/210 (61.90%), Query Frame = 3
Query:   72 SGNSAVNALKSKMQNLKTEIDELKSKIEEKDKLLQEEI-HKKS---SIDQEIETLNKKIRELESQTDELDEKLSLSSIELDEVSKRADINERTRRGLESRKQNNDSKICKLESQLQEXXXXXXXXXXXYDEISKALKDSEMKLAEAEDRSEAATKRLKETDSEMHGVSHTLKSMELNESKATLREENYEKQINKITLQLKDAQTRYEIQE 689
            +G+S++ A++ K+++L+ + D      EE+   LQ E+ H++    + + ++ +LN++I+ +E + D   E+L+ +  +L+E  K AD +ER  + +ESR Q ++ K+   E QL+EA ++   A++KY+E+++ L   E  L  AE+R+E +  +  E + E+  V++ LKS+E    K + +E+ YE++I  ++ +LK+A+TR E  E
Sbjct:    2 AGSSSLEAVRRKIRSLQEQAD----AAEERAGTLQRELDHERKLRETAEADVASLNRRIQLVEEELDRAQERLATALQKLEEAEKAADESERGMKVIESRAQKDEEKMEIQEIQLKEAKHIAEDADRKYEEVARKLVIIESDLERAEERAELSEGKCAELEEELKTVTNNLKSLEAQAEKYSQKEDRYEEEIKVLSDKLKEAETRAEFAE 207          
BLAST of SMED30023201 vs. Ensembl Human
Match: TPM3 (tropomyosin 3 [Source:HGNC Symbol;Acc:HGNC:12012])

HSP 1 Score: 77.7962 bits (190), Expect = 1.051e-15
Identity = 63/206 (30.58%), Postives = 123/206 (59.71%), Query Frame = 3
Query:   72 SGNSAVNALKSKMQNLKTEIDELKSKIEEKDKLLQEEIHKKSSIDQEIETLNKKIRELESQTDELDEKLSLSSIELDEVSKRADINERTRRGLESRKQNNDSKICKLESQLQEXXXXXXXXXXXYDEISKALKDSEMKLAEAEDRSEAATKRLKETDSEMHGVSHTLKSMELNESKATLREENYEKQINKITLQLKDAQTRYEIQE 689
            +G + + A+K K+Q L+ + D+ + + E   + ++ E   +   + E+ +LN++I+ +E + D   E+L+ +  +L+E  K AD +ER  + +E+R   ++ K+   E QL+EA ++   A++KY+E+++ L   E  L   E+R+E A  +  E + E+  V++ LKS+E    K + +E+ YE++I  +T +LK+A+TR E  E
Sbjct:    2 AGITTIEAVKRKIQVLQQQADDAEERAERLQREVEGERRAREQAEAEVASLNRRIQLVEEELDRAQERLATALQKLEEAEKAADESERGMKVIENRALKDEEKMELQEIQLKEAKHIAEEADRKYEEVARKLVIIEGDLERTEERAELAESKCSELEEELKNVTNNLKSLEAQAEKYSQKEDKYEEEIKILTDKLKEAETRAEFAE 207          
BLAST of SMED30023201 vs. Ensembl Celegans
Match: lev-11 (Tropomyosin isoforms a/b/d/f [Source:UniProtKB/Swiss-Prot;Acc:Q22866])

HSP 1 Score: 89.7373 bits (221), Expect = 2.231e-20
Identity = 67/214 (31.31%), Postives = 132/214 (61.68%), Query Frame = 3
Query:  171 LQEEIHKKSSIDQEIETLNKKIRELESQTDELDEKLSLSSIELDEVSKRADINERTRRGLESRKQNNDSKICKLESQLQEXXXXXXXXXXXYDEISKALKDSEMKLAEAEDRSEAATKRLKETDSEMHGVSHTLKSMELNESKATLREENYEKQINKITLQLKDAQTRYEIQESDYKRXXXXXXXXXXXXXXXXXXHYKLKNDLEKMMEEFQNI 812
            L+EE  K+   + E+  LN++I  +E   +  +++L  ++ +L++ +K AD  +R R+ +E+R Q ++ +   LE+Q+ EA  +   A++KY+E+++ L   E  L  AE+R+EA   ++ E + E+  V + LKS+E++E KA  RE++YE+QI  ++ +LK+A+TR E  E   ++    ++ LEE  + E     +L+++L+ M++E  ++
Sbjct:   43 LEEERKKREDAEAEVAALNRRIVLVEEDLERTEDRLKTATSKLEQATKAADEADRARKSMETRSQQDEERANFLETQVDEAKVIAEDADRKYEEVARKLAMVEADLERAEERAEAGENKIVELEEELRVVGNNLKSLEVSEEKALQREDSYEEQIRTVSSRLKEAETRAEFAERSVQKLQKEVDRLEELRDAEVLKARQLQDELDHMVQELNSV 256          
BLAST of SMED30023201 vs. Ensembl Celegans
Match: lev-11 (Tropomyosin isoforms a/b/d/f [Source:UniProtKB/Swiss-Prot;Acc:Q22866])

HSP 1 Score: 89.3521 bits (220), Expect = 3.443e-20
Identity = 59/173 (34.10%), Postives = 110/173 (63.58%), Query Frame = 3
Query:  171 LQEEIHKKSSIDQEIETLNKKIRELESQTDELDEKLSLSSIELDEVSKRADINERTRRGLESRKQNNDSKICKLESQLQEXXXXXXXXXXXYDEISKALKDSEMKLAEAEDRSEAATKRLKETDSEMHGVSHTLKSMELNESKATLREENYEKQINKITLQLKDAQTRYEIQE 689
            L+EE  K+   + E+  LN++I  +E   +  +++L  ++ +L++ +K AD  +R R+ +E+R Q ++ +   LE+Q+ EA  +   A++KY+E+++ L   E  L  AE+R+EA   ++ E + E+  V + LKS+E++E KA  RE++YE+QI  ++ +LK+A+TR E  E
Sbjct:   43 LEEERKKREDAEAEVAALNRRIVLVEEDLERTEDRLKTATSKLEQATKAADEADRARKSMETRSQQDEERANFLETQVDEAKVIAEDADRKYEEVARKLAMVEADLERAEERAEAGENKIVELEEELRVVGNNLKSLEVSEEKALQREDSYEEQIRTVSSRLKEAETRAEFAE 215          
BLAST of SMED30023201 vs. Ensembl Celegans
Match: lev-11 (Tropomyosin isoforms a/b/d/f [Source:UniProtKB/Swiss-Prot;Acc:Q22866])

HSP 1 Score: 88.9669 bits (219), Expect = 4.626e-20
Identity = 59/173 (34.10%), Postives = 110/173 (63.58%), Query Frame = 3
Query:  171 LQEEIHKKSSIDQEIETLNKKIRELESQTDELDEKLSLSSIELDEVSKRADINERTRRGLESRKQNNDSKICKLESQLQEXXXXXXXXXXXYDEISKALKDSEMKLAEAEDRSEAATKRLKETDSEMHGVSHTLKSMELNESKATLREENYEKQINKITLQLKDAQTRYEIQE 689
            L+EE  K+   + E+  LN++I  +E   +  +++L  ++ +L++ +K AD  +R R+ +E+R Q ++ +   LE+Q+ EA  +   A++KY+E+++ L   E  L  AE+R+EA   ++ E + E+  V + LKS+E++E KA  RE++YE+QI  ++ +LK+A+TR E  E
Sbjct:   43 LEEERKKREDAEAEVAALNRRIVLVEEDLERTEDRLKTATSKLEQATKAADEADRARKSMETRSQQDEERANFLETQVDEAKVIAEDADRKYEEVARKLAMVEADLERAEERAEAGENKIVELEEELRVVGNNLKSLEVSEEKALQREDSYEEQIRTVSSRLKEAETRAEFAE 215          
BLAST of SMED30023201 vs. Ensembl Celegans
Match: lev-11 (Tropomyosin isoforms a/b/d/f [Source:UniProtKB/Swiss-Prot;Acc:Q22866])

HSP 1 Score: 88.9669 bits (219), Expect = 4.626e-20
Identity = 59/173 (34.10%), Postives = 110/173 (63.58%), Query Frame = 3
Query:  171 LQEEIHKKSSIDQEIETLNKKIRELESQTDELDEKLSLSSIELDEVSKRADINERTRRGLESRKQNNDSKICKLESQLQEXXXXXXXXXXXYDEISKALKDSEMKLAEAEDRSEAATKRLKETDSEMHGVSHTLKSMELNESKATLREENYEKQINKITLQLKDAQTRYEIQE 689
            L+EE  K+   + E+  LN++I  +E   +  +++L  ++ +L++ +K AD  +R R+ +E+R Q ++ +   LE+Q+ EA  +   A++KY+E+++ L   E  L  AE+R+EA   ++ E + E+  V + LKS+E++E KA  RE++YE+QI  ++ +LK+A+TR E  E
Sbjct:   43 LEEERKKREDAEAEVAALNRRIVLVEEDLERTEDRLKTATSKLEQATKAADEADRARKSMETRSQQDEERANFLETQVDEAKVIAEDADRKYEEVARKLAMVEADLERAEERAEAGENKIVELEEELRVVGNNLKSLEVSEEKALQREDSYEEQIRTVSSRLKEAETRAEFAE 215          
BLAST of SMED30023201 vs. Ensembl Celegans
Match: lev-11 (Tropomyosin isoforms a/b/d/f [Source:UniProtKB/Swiss-Prot;Acc:Q22866])

HSP 1 Score: 85.1149 bits (209), Expect = 1.721e-18
Identity = 64/182 (35.16%), Postives = 117/182 (64.29%), Query Frame = 3
Query:  144 SKIEEKDKLLQEEIHKKSSIDQEIETLNKKIRELESQTDELDEKLSLSSIELDEVSKRADINERTRRGLESRKQNNDSKICKLESQLQEXXXXXXXXXXXYDEISKALKDSEMKLAEAEDRSEAATKRLKETDSEMHGVSHTLKSMELNESKATLREENYEKQINKITLQLKDAQTRYEIQE 689
            SK+EEK+K +QE        + E+ +LN+++  LE + +  +E+L +++ +L+E +   D +ER R+ +E+R Q ++ +   LE+Q+ EA  +   A++KY+E+++ L   E  L  AE+R+EA   ++ E + E+  V + LKS+E++E KA  RE++YE+QI  ++ +LK+A+TR E  E
Sbjct:   69 SKLEEKEKTVQE-------AEAEVASLNRRMTLLEEELERAEERLKIATEKLEEATHNVDESERARKSMETRSQQDEERANFLETQVDEAKVIAEDADRKYEEVARKLAMVEADLERAEERAEAGENKIVELEEELRVVGNNLKSLEVSEEKALQREDSYEEQIRTVSSRLKEAETRAEFAE 243          
BLAST of SMED30023201 vs. Ensembl Fly
Match: Tm1 (gene:FBgn0003721 transcript:FBtr0333917)

HSP 1 Score: 96.6709 bits (239), Expect = 1.063e-22
Identity = 69/194 (35.57%), Postives = 117/194 (60.31%), Query Frame = 3
Query:   78 NSAVNALKSKMQNLKTEIDELKSKIEEKDKLLQEEIHKKSSIDQEIETLNKKIRELESQTDELDEKLSLSSIELDEVSKRADINERTRRGLESRKQNNDSKICKLESQLQEXXXXXXXXXXXYDEISKALKDSEMKLAEAEDRSEAATKRLKETDSEMHGVSHTLKSMELNESKATLREENYEKQINKITLQLK 659
             + ++ LK KM+  K E+++ K + EE  K LQ E+ ++   + E+  LN++I+ LE   +  +E+L  ++ +L E S+ AD +ER R+ LE+R    D K+  LE+QL +A  +   A+KKY+E+++ L   E  L  AE+R+E    ++ E + E+  V + LKS+E++E KA  REE Y+ QI  +  +LK
Sbjct:    8 GTLLDVLKKKMRQTKEEMEKYKDECEEFHKRLQLEVVRREEAESEVAALNRRIQLLEEDLERSEERLGSATAKLSEASQAADESERIRKALENRTNMEDDKVALLENQLAQAKLIAEEADKKYEEVARKLAMVEADLERAEERAEQGENKIVELEEELRVVGNNLKSLEVSEEKANQREEEYKNQIKTLNTRLK 201          
BLAST of SMED30023201 vs. Ensembl Fly
Match: Tm1 (gene:FBgn0003721 transcript:FBtr0305656)

HSP 1 Score: 90.1225 bits (222), Expect = 1.796e-20
Identity = 67/194 (34.54%), Postives = 117/194 (60.31%), Query Frame = 3
Query:   78 NSAVNALKSKMQNLKTEIDELKSKIEEKDKLLQEEIHKKSSIDQEIETLNKKIRELESQTDELDEKLSLSSIELDEVSKRADINERTRRGLESRKQNNDSKICKLESQLQEXXXXXXXXXXXYDEISKALKDSEMKLAEAEDRSEAATKRLKETDSEMHGVSHTLKSMELNESKATLREENYEKQINKITLQLK 659
             + ++ LK KM+  K E+++ K + EE  K LQ E+ ++   + E+  LN++I+ LE   +  +E+L  ++ +L E S+ AD +ER R+ LE+R    D K+  LE+QL +A  +   A+KKY+E+++ L   E  L  +E++ E +  ++ E + E+  V + LKS+E++E KA  REE Y+ QI  +  +LK
Sbjct:    8 GTLLDVLKKKMRQTKEEMEKYKDECEEFHKRLQLEVVRREEAESEVAALNRRIQLLEEDLERSEERLGSATAKLSEASQAADESERIRKALENRTNMEDDKVALLENQLAQAKLIAEEADKKYEEVARKLVLMEQDLERSEEKVELSESKIVELEEELRVVGNNLKSLEVSEEKANQREEEYKNQIKTLNTRLK 201          
BLAST of SMED30023201 vs. Ensembl Fly
Match: Tm1 (gene:FBgn0003721 transcript:FBtr0089960)

HSP 1 Score: 89.3521 bits (220), Expect = 4.271e-20
Identity = 67/194 (34.54%), Postives = 117/194 (60.31%), Query Frame = 3
Query:   78 NSAVNALKSKMQNLKTEIDELKSKIEEKDKLLQEEIHKKSSIDQEIETLNKKIRELESQTDELDEKLSLSSIELDEVSKRADINERTRRGLESRKQNNDSKICKLESQLQEXXXXXXXXXXXYDEISKALKDSEMKLAEAEDRSEAATKRLKETDSEMHGVSHTLKSMELNESKATLREENYEKQINKITLQLK 659
             + ++ LK KM+  K E+++ K + EE  K LQ E+ ++   + E+  LN++I+ LE   +  +E+L  ++ +L E S+ AD +ER R+ LE+R    D K+  LE+QL +A  +   A+KKY+E+++ L   E  L  +E++ E +  ++ E + E+  V + LKS+E++E KAT +EE +E QI  +   LK
Sbjct:    8 GTLLDVLKKKMRQTKEEMEKYKDECEEFHKRLQLEVVRREEAESEVAALNRRIQLLEEDLERSEERLGSATAKLSEASQAADESERIRKALENRTNMEDDKVALLENQLAQAKLIAEEADKKYEEVARKLVLMEQDLERSEEKVELSESKIVELEEELRVVGNNLKSLEVSEEKATQKEETFETQIKVLDHSLK 201          
BLAST of SMED30023201 vs. Ensembl Fly
Match: Tm1 (gene:FBgn0003721 transcript:FBtr0333918)

HSP 1 Score: 88.9669 bits (219), Expect = 1.037e-19
Identity = 68/195 (34.87%), Postives = 115/195 (58.97%), Query Frame = 3
Query:   96 LKSKMQNLKTEIDE-------LKSKIEEKDKLLQEEIHKKSSIDQEIETLNKKIRELESQTDELDEKLSLSSIELDEVSKRADINERTRRGLESRKQNNDSKICKLESQLQEXXXXXXXXXXXYDEISKALKDSEMKLAEAEDRSEAATKRLKETDSEMHGVSHTLKSMELNESKATLREENYEKQINKITLQLK 659
            L+ K+Q ++ E+D+       +  K+EEK+K LQ       + + E+  LN++I+ LE   +  +E+L  ++ +L E S+ AD +ER R+ LE+R    D K+  LE+QL +A  +   A+KKY+E+++ L   E  L  AE+R+E    ++ E + E+  V + LKS+E++E KA  REE Y+ QI  +  +LK
Sbjct:   46 LQKKIQTVENELDQTQEALTLVTGKLEEKNKALQ-------NAESEVAALNRRIQLLEEDLERSEERLGSATAKLSEASQAADESERIRKALENRTNMEDDKVALLENQLAQAKLIAEEADKKYEEVARKLAMVEADLERAEERAEQGENKIVELEEELRVVGNNLKSLEVSEEKANQREEEYKNQIKTLNTRLK 233          
BLAST of SMED30023201 vs. Ensembl Fly
Match: Tm1 (gene:FBgn0003721 transcript:FBtr0305654)

HSP 1 Score: 87.0409 bits (214), Expect = 4.648e-19
Identity = 69/195 (35.38%), Postives = 117/195 (60.00%), Query Frame = 3
Query:   96 LKSKMQNLKTEIDE-------LKSKIEEKDKLLQEEIHKKSSIDQEIETLNKKIRELESQTDELDEKLSLSSIELDEVSKRADINERTRRGLESRKQNNDSKICKLESQLQEXXXXXXXXXXXYDEISKALKDSEMKLAEAEDRSEAATKRLKETDSEMHGVSHTLKSMELNESKATLREENYEKQINKITLQLK 659
            L+ K+Q ++ E+D+       +  K+EEK+K LQ       + + E+  LN++I+ LE   +  +E+L  ++ +L E S+ AD +ER R+ LE+R   ++ ++  LE+QL+EA  L   A+KKYDE+++ L   E  L  AE+R+E    ++ E + E+  V + LKS+E++E KA  REE Y+ QI  +  +LK
Sbjct:   46 LQKKIQTVENELDQTQEALTLVTGKLEEKNKALQ-------NAESEVAALNRRIQLLEEDLERSEERLGSATAKLSEASQAADESERARKILENRALADEERMDALENQLKEARFLAEEADKKYDEVARKLAMVEADLERAEERAEQGENKIVELEEELRVVGNNLKSLEVSEEKANQREEEYKNQIKTLNTRLK 233          
BLAST of SMED30023201 vs. Ensembl Zebrafish
Match: tpm3 (tropomyosin 3 [Source:ZFIN;Acc:ZDB-GENE-030826-16])

HSP 1 Score: 83.9593 bits (206), Expect = 3.633e-18
Identity = 63/206 (30.58%), Postives = 130/206 (63.11%), Query Frame = 3
Query:   72 SGNSAVNALKSKMQNLKTEIDELKSKIEEKDKLLQEEIHKKSSIDQEIETLNKKIRELESQTDELDEKLSLSSIELDEVSKRADINERTRRGLESRKQNNDSKICKLESQLQEXXXXXXXXXXXYDEISKALKDSEMKLAEAEDRSEAATKRLKETDSEMHGVSHTLKSMELNESKATLREENYEKQINKITLQLKDAQTRYEIQE 689
            +G+++++A+K K++ L+ + DE + + E   + ++EE   +   + E+ +LN++I+ +E + D   E+L+ +  +L+E  K AD +ER  + +E+R   ++ K+   E QL+EA ++   A++KY+E+++ L   E +L   E+R+E A   +K+ + E+  +  TLK+++ +E K + +E+ YE++I  +T +LK+A+TR E  E
Sbjct:    2 AGSNSIDAVKRKIKVLQQQADEAEERAEILQRQVEEEKRAREQAEAEVASLNRRIQLVEEELDRAQERLATALQKLEEAEKAADESERGMKVIENRALKDEEKMELQEIQLKEAKHIAEEADRKYEEVARKLVIVEGELERTEERAELAESHVKQMEEELRALDQTLKTLQASEEKYSQKEDKYEEEIKILTDKLKEAETRAEFAE 207          
BLAST of SMED30023201 vs. Ensembl Zebrafish
Match: tpm3 (tropomyosin 3 [Source:ZFIN;Acc:ZDB-GENE-030826-16])

HSP 1 Score: 82.0333 bits (201), Expect = 1.851e-17
Identity = 64/206 (31.07%), Postives = 128/206 (62.14%), Query Frame = 3
Query:   72 SGNSAVNALKSKMQNLKTEIDELKSKIEEKDKLLQEEIHKKSSIDQEIETLNKKIRELESQTDELDEKLSLSSIELDEVSKRADINERTRRGLESRKQNNDSKICKLESQLQEXXXXXXXXXXXYDEISKALKDSEMKLAEAEDRSEAATKRLKETDSEMHGVSHTLKSMELNESKATLREENYEKQINKITLQLKDAQTRYEIQE 689
            +G+++++A+K K++ L+ + DE + + E   + ++EE   +   + E+ +LN++I+ +E + D   E+L+ +  +L+E  K AD +ER  + +E+R   ++ K+   E QL+EA ++   A++KY+E+++ L   E +L   E+R+E A  +  E + E+  V++ LKS+E    K + +E+ YE++I  +T +LK+A+TR E  E
Sbjct:    2 AGSNSIDAVKRKIKVLQQQADEAEERAEILQRQVEEEKRAREQAEAEVASLNRRIQLVEEELDRAQERLATALQKLEEAEKAADESERGMKVIENRALKDEEKMELQEIQLKEAKHIAEEADRKYEEVARKLVIVEGELERTEERAELAESKCAELEEELKNVTNNLKSLEAQAEKYSQKEDKYEEEIKILTDKLKEAETRAEFAE 207          
BLAST of SMED30023201 vs. Ensembl Zebrafish
Match: tpm2 (tropomyosin 2 (beta) [Source:ZFIN;Acc:ZDB-GENE-040625-114])

HSP 1 Score: 76.6406 bits (187), Expect = 1.412e-15
Identity = 66/212 (31.13%), Postives = 127/212 (59.91%), Query Frame = 3
Query:   66 MSSGNSAVNALKSKMQNLKTEIDELKSKIEEKDKLLQEEI----HKKSSIDQEIETLNKKIRELESQTDELDEKLSLSSIELDEVSKRADINERTRRGLESRKQNNDSKICKLESQLQEXXXXXXXXXXXYDEISKALKDSEMKLAEAEDRSEAATKRLKETDSEMHGVSHTLKSMELNESKATLREENYEKQINKITLQLKDAQTRYEIQE 689
            M+S  S + A+K K+Q L+ + D+ + + E    +LQ E+      +   + E+ +LN++I+ +E + D   E+L+ +  +L+E  K AD +ER  + +E+R   ++ K+   E QL+EA ++   A++KY+E+++ L   E +L  +E+R+E A  R+++ + E+  +   LKSM   E + + +E+ YE++I  +T +LK+A+TR E  E
Sbjct:    1 MASITS-IEAVKRKIQTLQQQADDAEDRAE----MLQREVDLERQCRERAEAEVASLNRRIQLVEEELDRAQERLATALQKLEEAEKAADESERGMKVIENRATKDEEKMEIQEMQLKEAKHIAEEADRKYEEVARKLVILEGELERSEERAEVAEARVRQLEEELRLMDQNLKSMMAGEEEYSTKEDKYEEEIRVLTDKLKEAETRAEFAE 207          
BLAST of SMED30023201 vs. Ensembl Zebrafish
Match: tpma (alpha-tropomyosin [Source:NCBI gene;Acc:30324])

HSP 1 Score: 73.1738 bits (178), Expect = 1.378e-14
Identity = 60/206 (29.13%), Postives = 126/206 (61.17%), Query Frame = 3
Query:   72 SGNSAVNALKSKMQNLKTEIDELKSKIEEKDKLLQEEIHKKSSIDQEIETLNKKIRELESQTDELDEKLSLSSIELDEVSKRADINERTRRGLESRKQNNDSKICKLESQLQEXXXXXXXXXXXYDEISKALKDSEMKLAEAEDRSEAATKRLKETDSEMHGVSHTLKSMELNESKATLREENYEKQINKITLQLKDAQTRYEIQE 689
            +G + ++A+K K+++L+ + ++ +S+     + L+ E   + S + ++ +LN++I+ +E + D   E+L+ +  +L+E  K AD +ER  + +E+R   ++ K+   E QL+EA ++   A++KY+E+++ L   E +L   E+R+E    +  E + E+  V++ +KS+E    K + +E+ YE++I  +T +LK+A+TR E  E
Sbjct:    2 AGTAYLDAVKKKIRSLQEQAEDAESRTLRLQEQLESERATRRSAEGDVASLNRRIQLVEEELDRAQERLATALQKLEEAEKAADESERGMKVIENRALKDEEKMELQEIQLKEAKHIAEEADRKYEEVARKLVIVEGELERTEERAELNEGKCSELEEELKTVTNNMKSLEAQAEKYSAKEDKYEEEIKVLTDKLKEAETRAEFAE 207          
BLAST of SMED30023201 vs. Ensembl Zebrafish
Match: tpm3 (tropomyosin 3 [Source:ZFIN;Acc:ZDB-GENE-030826-16])

HSP 1 Score: 72.4034 bits (176), Expect = 7.628e-14
Identity = 58/172 (33.72%), Postives = 108/172 (62.79%), Query Frame = 3
Query:  180 EIHKKSSIDQEIE--TLNKKIRELESQTDELDEKLSLSSIELDEVSKRADINERTRRGLESRKQNNDSKICKLESQLQEXXXXXXXXXXXYDEISKALKDSEMKLAEAEDRSEAATKRLKETDSEMHGVSHTLKSMELNESKATLREENYEKQINKITLQLKDAQTRYEIQE 689
            EI +K + D E E  +LN++I+ +E + D   E+L+ +  +L+E  K AD +ER  + +E+R   ++ K+   E QL+EA ++   A++KY+E+++ L   E +L   E+R+E A   +K+ + E+  +  TLK+++ +E K + +E+ YE++I  +T +LK+A+TR E  E
Sbjct:   72 EISEKKAADAEAEVASLNRRIQLVEEELDRAQERLATALQKLEEAEKAADESERGMKVIENRALKDEEKMELQEIQLKEAKHIAEEADRKYEEVARKLVIVEGELERTEERAELAESHVKQMEEELRALDQTLKTLQASEEKYSQKEDKYEEEIKILTDKLKEAETRAEFAE 243          
BLAST of SMED30023201 vs. Ensembl Xenopus
Match: tpm1 (tropomyosin 1 [Source:NCBI gene;Acc:448541])

HSP 1 Score: 71.633 bits (174), Expect = 1.213e-13
Identity = 60/206 (29.13%), Postives = 125/206 (60.68%), Query Frame = 3
Query:   72 SGNSAVNALKSKMQNLKTEIDELKSKIEEKDKLLQEEIHKKSSIDQEIETLNKKIRELESQTDELDEKLSLSSIELDEVSKRADINERTRRGLESRKQNNDSKICKLESQLQEXXXXXXXXXXXYDEISKALKDSEMKLAEAEDRSEAATKRLKETDSEMHGVSHTLKSMELNESKATLREENYEKQINKITLQLKDAQTRYEIQE 689
            +G +++ A+K K++ L+ + D  + + E+  +    E  ++ + + ++ +LN++I+ +E + D   E+L+ +  +L+E  K AD +ER  + +E+R   ++ K+   E QL+EA ++   A++KY+E+++ L   E  L  AE+R+E +  R ++ D ++  +  TLK++   E K + +E+ YE++I  +T +LK+A+TR E  E
Sbjct:    5 AGITSLEAVKRKIKCLQDQADAAEERAEKLQRERDMERKQREAAEGDVASLNRRIQLVEEELDRAQERLATALQKLEEAEKAADESERGMKVIENRALKDEEKMELQEIQLKEAKHIAEEADRKYEEVARKLVIIEGDLERAEERAELSESRYRQLDDQLRIMDQTLKTLMAAEEKYSQKEDKYEEEIKVLTDKLKEAETRAEFAE 210          
BLAST of SMED30023201 vs. Ensembl Xenopus
Match: tpm1 (tropomyosin 1 [Source:NCBI gene;Acc:448541])

HSP 1 Score: 70.4774 bits (171), Expect = 5.671e-13
Identity = 61/206 (29.61%), Postives = 125/206 (60.68%), Query Frame = 3
Query:   72 SGNSAVNALKSKMQNLKTEIDELKSKIEEKDKLLQEEIHKKSSIDQEIETLNKKIRELESQTDELDEKLSLSSIELDEVSKRADINERTRRGLESRKQNNDSKICKLESQLQEXXXXXXXXXXXYDEISKALKDSEMKLAEAEDRSEAATKRLKETDSEMHGVSHTLKSMELNESKATLREENYEKQINKITLQLKDAQTRYEIQE 689
            +G +++ A+K K++ L+ + D  + + E+  +    E  ++ + + ++ +LN++I+ +E + D   E+L+ +  +L+E  K AD +ER  + +E+R   ++ K+   E QL+EA ++   A++KY+E+++ L   E  L  AE+R+E +  +  E + E+  V++ LKS+E    K + +E+ YE++I  +T +LK+A+TR E  E
Sbjct:    2 AGITSLEAVKRKIKCLQDQADAAEERAEKLQRERDMERKQREAAEGDVASLNRRIQLVEEELDRAQERLATALQKLEEAEKAADESERGMKVIENRALKDEEKMELQEIQLKEAKHIAEEADRKYEEVARKLVIIEGDLERAEERAELSESKCAELEEELKTVTNNLKSLEAQAEKYSQKEDKYEEEIKVLTDKLKEAETRAEFAE 207          
BLAST of SMED30023201 vs. Ensembl Xenopus
Match: tpm1 (tropomyosin 1 [Source:NCBI gene;Acc:448541])

HSP 1 Score: 70.0922 bits (170), Expect = 6.006e-13
Identity = 64/201 (31.84%), Postives = 121/201 (60.20%), Query Frame = 3
Query:   93 ALKSKMQNLKTEIDELKSKIEEKDKLLQEEIHKKSSIDQE--IETLNKKIRELESQTDELDEKLSLSSIELDEVSKRADINERTRRGLESRKQNNDSKICKLESQLQEXXXXXXXXXXXYDEISKALKDSEMKLAEAEDRSEAATKRLKETDSEMHGVSHTLKSMELNESKATLREENYEKQINKITLQLKDAQTRYEIQE 689
            AL+ K++  + E+D+    +  KD   + E+ +K + D E  + +LN++I+ +E + D   E+L+ +  +L+E  K AD +ER  + +E+R   ++ K+   E QL+EA ++   A++KY+E+++ L   E  L  AE+R+E +  +  E + E+  V++ LKS+E    K + +E+ YE++I  +T +LK+A+TR E  E
Sbjct:   45 ALQKKLKGTEDELDKYSEAL--KDAQEKLELAEKKATDAEGDVASLNRRIQLVEEELDRAQERLATALQKLEEAEKAADESERGMKVIENRALKDEEKMELQEIQLKEAKHIAEEADRKYEEVARKLVIIEGDLERAEERAELSESKCAELEEELKTVTNNLKSLEAQAEKYSQKEDKYEEEIKVLTDKLKEAETRAEFAE 243          
BLAST of SMED30023201 vs. Ensembl Xenopus
Match: tpm1 (tropomyosin 1 [Source:NCBI gene;Acc:448541])

HSP 1 Score: 69.707 bits (169), Expect = 8.572e-13
Identity = 64/201 (31.84%), Postives = 121/201 (60.20%), Query Frame = 3
Query:   93 ALKSKMQNLKTEIDELKSKIEEKDKLLQEEIHKKSSIDQE--IETLNKKIRELESQTDELDEKLSLSSIELDEVSKRADINERTRRGLESRKQNNDSKICKLESQLQEXXXXXXXXXXXYDEISKALKDSEMKLAEAEDRSEAATKRLKETDSEMHGVSHTLKSMELNESKATLREENYEKQINKITLQLKDAQTRYEIQE 689
            AL+ K++  + E+D+    +  KD   + E+ +K + D E  + +LN++I+ +E + D   E+L+ +  +L+E  K AD +ER  + +E+R   ++ K+   E QL+EA ++   A++KY+E+++ L   E  L  AE+R+E +  +  E + E+  V++ LKS+E    K + +E+ YE++I  +T +LK+A+TR E  E
Sbjct:   45 ALQKKLKGTEDELDKYSEAL--KDAQEKLELAEKKATDAEGDVASLNRRIQLVEEELDRAQERLATALQKLEEAEKAADESERGMKVIENRALKDEEKMELQEIQLKEAKHIAEEADRKYEEVARKLVIIEGDLERAEERAELSESKCAELEEELKTVTNNLKSLEAQAEKYSQKEDKYEEEIKVLTDKLKEAETRAEFAE 243          
BLAST of SMED30023201 vs. Ensembl Xenopus
Match: tpm1 (tropomyosin 1 [Source:NCBI gene;Acc:448541])

HSP 1 Score: 69.707 bits (169), Expect = 8.967e-13
Identity = 64/201 (31.84%), Postives = 121/201 (60.20%), Query Frame = 3
Query:   93 ALKSKMQNLKTEIDELKSKIEEKDKLLQEEIHKKSSIDQE--IETLNKKIRELESQTDELDEKLSLSSIELDEVSKRADINERTRRGLESRKQNNDSKICKLESQLQEXXXXXXXXXXXYDEISKALKDSEMKLAEAEDRSEAATKRLKETDSEMHGVSHTLKSMELNESKATLREENYEKQINKITLQLKDAQTRYEIQE 689
            AL+ K++  + E+D+    +  KD   + E+ +K + D E  + +LN++I+ +E + D   E+L+ +  +L+E  K AD +ER  + +E+R   ++ K+   E QL+EA ++   A++KY+E+++ L   E  L  AE+R+E +  +  E + E+  V++ LKS+E    K + +E+ YE++I  +T +LK+A+TR E  E
Sbjct:   57 ALQKKLKGTEDELDKYSEAL--KDAQEKLELAEKKATDAEGDVASLNRRIQLVEEELDRAQERLATALQKLEEAEKAADESERGMKVIENRALKDEEKMELQEIQLKEAKHIAEEADRKYEEVARKLVIIEGDLERAEERAELSESKCAELEEELKTVTNNLKSLEAQAEKYSQKEDKYEEEIKVLTDKLKEAETRAEFAE 255          
BLAST of SMED30023201 vs. Ensembl Mouse
Match: Tpm3 (tropomyosin 3, gamma [Source:MGI Symbol;Acc:MGI:1890149])

HSP 1 Score: 78.9518 bits (193), Expect = 2.400e-16
Identity = 63/206 (30.58%), Postives = 123/206 (59.71%), Query Frame = 3
Query:   72 SGNSAVNALKSKMQNLKTEIDELKSKIEEKDKLLQEEIHKKSSIDQEIETLNKKIRELESQTDELDEKLSLSSIELDEVSKRADINERTRRGLESRKQNNDSKICKLESQLQEXXXXXXXXXXXYDEISKALKDSEMKLAEAEDRSEAATKRLKETDSEMHGVSHTLKSMELNESKATLREENYEKQINKITLQLKDAQTRYEIQE 689
            +G + + A+K K+Q L+ + D+ + + E   + ++ E   +   + E+ +LN++I+ +E + D   E+L+ +  +L+E  K AD +ER  + +E+R   ++ K+   E QL+EA ++   A++KY+E+++ L   E  L   E+R+E A  +  E + E+  V++ LKS+E    K + +E+ YE++I  +T +LK+A+TR E  E
Sbjct:    2 AGTTTIEAVKRKIQVLQQQADDAEERAERLQREVEGERRAREQAEAEVASLNRRIQLVEEELDRAQERLATALQKLEEAEKAADESERGMKVIENRALKDEEKMELQEIQLKEAKHIAEEADRKYEEVARKLVIIEGDLERTEERAELAESKCSELEEELKNVTNNLKSLEAQAEKYSQKEDKYEEEIKILTDKLKEAETRAEFAE 207          
BLAST of SMED30023201 vs. Ensembl Mouse
Match: Tpm3 (tropomyosin 3, gamma [Source:MGI Symbol;Acc:MGI:1890149])

HSP 1 Score: 78.9518 bits (193), Expect = 2.486e-16
Identity = 63/206 (30.58%), Postives = 123/206 (59.71%), Query Frame = 3
Query:   72 SGNSAVNALKSKMQNLKTEIDELKSKIEEKDKLLQEEIHKKSSIDQEIETLNKKIRELESQTDELDEKLSLSSIELDEVSKRADINERTRRGLESRKQNNDSKICKLESQLQEXXXXXXXXXXXYDEISKALKDSEMKLAEAEDRSEAATKRLKETDSEMHGVSHTLKSMELNESKATLREENYEKQINKITLQLKDAQTRYEIQE 689
            +G + + A+K K+Q L+ + D+ + + E   + ++ E   +   + E+ +LN++I+ +E + D   E+L+ +  +L+E  K AD +ER  + +E+R   ++ K+   E QL+EA ++   A++KY+E+++ L   E  L   E+R+E A  +  E + E+  V++ LKS+E    K + +E+ YE++I  +T +LK+A+TR E  E
Sbjct:    2 AGTTTIEAVKRKIQVLQQQADDAEERAERLQREVEGERRAREQAEAEVASLNRRIQLVEEELDRAQERLATALQKLEEAEKAADESERGMKVIENRALKDEEKMELQEIQLKEAKHIAEEADRKYEEVARKLVIIEGDLERTEERAELAESKCSELEEELKNVTNNLKSLEAQAEKYSQKEDKYEEEIKILTDKLKEAETRAEFAE 207          
BLAST of SMED30023201 vs. Ensembl Mouse
Match: Tpm1 (tropomyosin 1, alpha [Source:MGI Symbol;Acc:MGI:98809])

HSP 1 Score: 78.1814 bits (191), Expect = 5.123e-16
Identity = 65/210 (30.95%), Postives = 129/210 (61.43%), Query Frame = 3
Query:   72 SGNSAVNALKSKMQNLKTEIDELKSKIEEKDKLLQEEIHK----KSSIDQEIETLNKKIRELESQTDELDEKLSLSSIELDEVSKRADINERTRRGLESRKQNNDSKICKLESQLQEXXXXXXXXXXXYDEISKALKDSEMKLAEAEDRSEAATKRLKETDSEMHGVSHTLKSMELNESKATLREENYEKQINKITLQLKDAQTRYEIQE 689
            +G+S++ A++ K+++L+ + D      EE+   LQ E+ +    + + + ++ +LN++I+ +E + D   E+L+ +  +L+E  K AD +ER  + +ESR Q ++ K+   E QL+EA ++   A++KY+E+++ L   E  L  AE+R+E +  +  E + E+  V++ LKS+E    K + +E+ YE++I  ++ +LK+A+TR E  E
Sbjct:    2 AGSSSLEAVRRKIRSLQEQAD----AAEERAGSLQRELDQERKLRETAEADVASLNRRIQLVEEELDRAQERLATALQKLEEAEKAADESERGMKVIESRAQKDEEKMEIQEIQLKEAKHIAEDADRKYEEVARKLVIIESDLERAEERAELSEGKCAELEEELKTVTNNLKSLEAQAEKYSQKEDKYEEEIKVLSDKLKEAETRAEFAE 207          
BLAST of SMED30023201 vs. Ensembl Mouse
Match: Tpm1 (tropomyosin 1, alpha [Source:MGI Symbol;Acc:MGI:98809])

HSP 1 Score: 77.7962 bits (190), Expect = 5.708e-16
Identity = 65/210 (30.95%), Postives = 129/210 (61.43%), Query Frame = 3
Query:   72 SGNSAVNALKSKMQNLKTEIDELKSKIEEKDKLLQEEIHK----KSSIDQEIETLNKKIRELESQTDELDEKLSLSSIELDEVSKRADINERTRRGLESRKQNNDSKICKLESQLQEXXXXXXXXXXXYDEISKALKDSEMKLAEAEDRSEAATKRLKETDSEMHGVSHTLKSMELNESKATLREENYEKQINKITLQLKDAQTRYEIQE 689
            +G+S++ A++ K+++L+ + D      EE+   LQ E+ +    + + + ++ +LN++I+ +E + D   E+L+ +  +L+E  K AD +ER  + +ESR Q ++ K+   E QL+EA ++   A++KY+E+++ L   E  L  AE+R+E +  +  E + E+  V++ LKS+E    K + +E+ YE++I  ++ +LK+A+TR E  E
Sbjct:    2 AGSSSLEAVRRKIRSLQEQAD----AAEERAGSLQRELDQERKLRETAEADVASLNRRIQLVEEELDRAQERLATALQKLEEAEKAADESERGMKVIESRAQKDEEKMEIQEIQLKEAKHIAEDADRKYEEVARKLVIIESDLERAEERAELSEGKCAELEEELKTVTNNLKSLEAQAEKYSQKEDKYEEEIKVLSDKLKEAETRAEFAE 207          
BLAST of SMED30023201 vs. Ensembl Mouse
Match: Tpm3 (tropomyosin 3, gamma [Source:MGI Symbol;Acc:MGI:1890149])

HSP 1 Score: 77.7962 bits (190), Expect = 6.785e-16
Identity = 62/206 (30.10%), Postives = 121/206 (58.74%), Query Frame = 3
Query:   72 SGNSAVNALKSKMQNLKTEIDELKSKIEEKDKLLQEEIHKKSSIDQEIETLNKKIRELESQTDELDEKLSLSSIELDEVSKRADINERTRRGLESRKQNNDSKICKLESQLQEXXXXXXXXXXXYDEISKALKDSEMKLAEAEDRSEAATKRLKETDSEMHGVSHTLKSMELNESKATLREENYEKQINKITLQLKDAQTRYEIQE 689
            +G + + A+K K+Q L+ + D+ + + E   + ++ E   +   + E+ +LN++I+ +E + D   E+L+ +  +L+E  K AD +ER  + +E+R   ++ K+   E QL+EA ++   A++KY+E+++ L   E  L   E+R+E A  R +E D ++  +   LK +   E K + +E+ YE++I  +T +LK+A+TR E  E
Sbjct:    2 AGTTTIEAVKRKIQVLQQQADDAEERAERLQREVEGERRAREQAEAEVASLNRRIQLVEEELDRAQERLATALQKLEEAEKAADESERGMKVIENRALKDEEKMELQEIQLKEAKHIAEEADRKYEEVARKLVIIEGDLERTEERAELAESRCREMDEQIRLMDQNLKCLSAAEEKYSQKEDKYEEEIKILTDKLKEAETRAEFAE 207          
BLAST of SMED30023201 vs. UniProt/SwissProt
Match: sp|Q8WR63|TPM_TRIPS (Tropomyosin OS=Trichinella pseudospiralis OX=6337 PE=2 SV=1)

HSP 1 Score: 100.523 bits (249), Expect = 8.145e-23
Identity = 69/195 (35.38%), Postives = 126/195 (64.62%), Query Frame = 3
Query:  105 KMQNLKTEIDELKSKIEEKDKLLQEEIHKKSSIDQEIETLNKKIRELESQTDELDEKLSLSSIELDEVSKRADINERTRRGLESRKQNNDSKICKLESQLQEXXXXXXXXXXXYDEISKALKDSEMKLAEAEDRSEAATKRLKETDSEMHGVSHTLKSMELNESKATLREENYEKQINKITLQLKDAQTRYEIQE 689
            KM  ++ E+D+ + ++   +  L+E+  K    + E+  LN++I+ LE   +  +E+L +++ +L+E S+ AD +ER R+ +E+R   ++ ++ +LE+QL+EA  L   A++KYDE+++ L   E  L  AE+R+EA   ++ E + E+  V + LKS+E++E KA  RE++YE+QI  +T +LK+A+TR E  E
Sbjct:   49 KMMQVENELDKAQEELTGANAQLEEKEKKVQEAEAEVAALNRRIQLLEEDFERAEERLKIATEKLEEASQTADESERVRKVMENRSLQDEERVYQLEAQLKEAQLLAEEADRKYDEVARKLAMVEADLERAEERAEAGENKIVELEEELRVVGNNLKSLEVSEEKALQREDSYEEQIRLLTQRLKEAETRAEFAE 243          
BLAST of SMED30023201 vs. UniProt/SwissProt
Match: sp|Q95VA8|TPM_TRISP (Tropomyosin OS=Trichinella spiralis OX=6334 PE=2 SV=1)

HSP 1 Score: 99.7525 bits (247), Expect = 1.621e-22
Identity = 69/195 (35.38%), Postives = 125/195 (64.10%), Query Frame = 3
Query:  105 KMQNLKTEIDELKSKIEEKDKLLQEEIHKKSSIDQEIETLNKKIRELESQTDELDEKLSLSSIELDEVSKRADINERTRRGLESRKQNNDSKICKLESQLQEXXXXXXXXXXXYDEISKALKDSEMKLAEAEDRSEAATKRLKETDSEMHGVSHTLKSMELNESKATLREENYEKQINKITLQLKDAQTRYEIQE 689
            KM  ++ E+D+ + ++   +  L+E+  K    + E+  LN++I+ LE   +  +E+L +++ +L E S+ AD +ER R+ +E+R   ++ ++ +LE+QL+EA  L   A++KYDE+++ L   E  L  AE+R+EA   ++ E + E+  V + LKS+E++E KA  RE++YE+QI  +T +LK+A+TR E  E
Sbjct:   49 KMMQVENELDKAQEELTGANAQLEEKEKKVQEAEAEVAALNRRIQLLEEDFERAEERLIIATEKLGEASQTADESERVRKVMENRSLQDEERVYQLEAQLKEAQLLAEEADRKYDEVARKLAMVEADLERAEERAEAGENKIVELEEELRVVGNNLKSLEVSEEKALQREDSYEEQIRLLTQRLKEAETRAEFAE 243          
BLAST of SMED30023201 vs. UniProt/SwissProt
Match: sp|Q23939|TPM_DERFA (Tropomyosin OS=Dermatophagoides farinae OX=6954 PE=1 SV=2)

HSP 1 Score: 95.5153 bits (236), Expect = 5.128e-21
Identity = 69/198 (34.85%), Postives = 124/198 (62.63%), Query Frame = 3
Query:   87 VNALKSKMQNLKTEIDELK-------SKIEEKDKLLQEEIHKKSSIDQEIETLNKKIRELESQTDELDEKLSLSSIELDEVSKRADINERTRRGLESRKQNNDSKICKLESQLQEXXXXXXXXXXXYDEISKALKDSEMKLAEAEDRSEAATKRLKETDSEMHGVSHTLKSMELNESKATLREENYEKQINKITLQLK 659
            V AL+ K+Q ++ E+D+++       +K+EEK+K LQ       + + ++  LN++I+ +E   +  +E+L +++ +L+E S+ AD +ER R+ LE R   ++ ++  LE+QL+EA  +   A++KYDE+++ L   E  L  AE+R+E    ++ E + E+  V + LKS+E++E KA  REE YE+QI  +T +LK
Sbjct:   43 VRALQKKIQQIENELDQVQEQLSAANTKLEEKEKALQ-------TAEGDVAALNRRIQLIEEDLERSEERLKIATAKLEEASQSADESERMRKMLEHRSITDEERMDGLENQLKEARMMAEDADRKYDEVARKLAMVEADLERAEERAETGESKIVELEEELRVVGNNLKSLEVSEEKAQQREEAYEQQIRIMTAKLK 233          
BLAST of SMED30023201 vs. UniProt/SwissProt
Match: sp|P0DSM7|TPM02_PERAM (Tropomyosin Per a 7.0102 OS=Periplaneta americana OX=6978 PE=1 SV=1)

HSP 1 Score: 93.9745 bits (232), Expect = 1.815e-20
Identity = 69/196 (35.20%), Postives = 124/196 (63.27%), Query Frame = 3
Query:   93 ALKSKMQNLKTEIDE-------LKSKIEEKDKLLQEEIHKKSSIDQEIETLNKKIRELESQTDELDEKLSLSSIELDEVSKRADINERTRRGLESRKQNNDSKICKLESQLQEXXXXXXXXXXXYDEISKALKDSEMKLAEAEDRSEAATKRLKETDSEMHGVSHTLKSMELNESKATLREENYEKQINKITLQLK 659
            +L+ K+Q ++ ++D+       + +K++EKDK LQ       + + E+  LN++I+ LE   +  +E+L+ ++ +L E S+ AD +ER R+ LES+   ++ ++  LE+QL+EA  +   A+KKYDE+++ L   E  L  AE+R+E+   ++ E + E+  V + LKS+E++E KA LREE Y++QI  +T +LK
Sbjct:   45 SLQKKIQQIENDLDQTMEQLMQVNAKLDEKDKALQ-------NAESEVAALNRRIQLLEEDLERSEERLATATAKLAEASQAADESERARKILESKGLADEERMDALENQLKEARFMAEEADKKYDEVARKLAMVEADLERAEERAESGESKIVELEEELRVVGNNLKSLEVSEEKANLREEEYKQQIKTLTTRLK 233          
BLAST of SMED30023201 vs. UniProt/SwissProt
Match: sp|Q8T6L5|TPM_PERFU (Tropomyosin OS=Periplaneta fuliginosa OX=36977 PE=2 SV=1)

HSP 1 Score: 93.5893 bits (231), Expect = 2.063e-20
Identity = 69/196 (35.20%), Postives = 124/196 (63.27%), Query Frame = 3
Query:   93 ALKSKMQNLKTEIDE-------LKSKIEEKDKLLQEEIHKKSSIDQEIETLNKKIRELESQTDELDEKLSLSSIELDEVSKRADINERTRRGLESRKQNNDSKICKLESQLQEXXXXXXXXXXXYDEISKALKDSEMKLAEAEDRSEAATKRLKETDSEMHGVSHTLKSMELNESKATLREENYEKQINKITLQLK 659
            +L+ K+Q ++ ++D+       + +K++EKDK LQ       + + E+  LN++I+ LE   +  +E+L+ ++ +L E S+ AD +ER R+ LES+   ++ ++  LE+QL+EA  +   A+KKYDE+++ L   E  L  AE+R+E+   ++ E + E+  V + LKS+E++E KA LREE Y++QI  +T +LK
Sbjct:   45 SLQKKIQQIENDLDQTMEQLMQVNAKLDEKDKALQ-------NAESEVAALNRRIQLLEEDLERSEERLATATAKLAEASQAADESERARKILESKGLADEERMDALENQLKEARFMAEEADKKYDEVARKLAMVEADLERAEERAESGESKIVELEEELRVVGNNLKSLEVSEEKANLREEAYKQQIKTLTTRLK 233          
BLAST of SMED30023201 vs. TrEMBL
Match: Q5C3B4 (SJCHGC02287 protein (Fragment) OS=Schistosoma japonicum OX=6182 PE=2 SV=1)

HSP 1 Score: 130.183 bits (326), Expect = 5.190e-32
Identity = 82/204 (40.20%), Postives = 129/204 (63.24%), Query Frame = 3
Query:   66 MSSGNSAVNALKSKMQNLKTEIDELKSKIEEKDKLLQEEIHKKSSIDQEIETLNKKIRELESQTDELDEKLSLSSIELDEVSKRADINERTRRGLESRKQNNDSKICKLESQLQEXXXXXXXXXXXYDEISKALKDSEMKLAEAEDRSEAATKRLKETDSEMHGVSHTLKSMELNESKATLREENYEKQINKITLQLKDAQTRY 677
            MSS    + A+KSKMQ +K +ID+LK ++  K  +L++E   K+  + E+ +L K+IR+LE + +  + +L  ++++L+E SK AD ++R RR LE+R+   D +I +LES +QE       A  KY+E ++ L  +E+ L+ AEDR EAA  RLKE  S +HG    +K++E  ES+A  REE YE+ I  +T +LK A+ R 
Sbjct:    1 MSS--PVIGAVKSKMQGMKLQIDQLKQEVSSKQAVLRKEEENKTKAEAEVASLQKRIRQLEDELESTETRLQEATLKLEEASKAADESDRARRVLEARQTAEDERILQLESMVQETAKSVKDAETKYEEATRKLAVAEVALSHAEDRIEAAESRLKELQSIIHGTMGPVKTLEHQESQAAQREEAYEENIRDLTERLKAAERRV 202          
BLAST of SMED30023201 vs. TrEMBL
Match: A0A183L9D1 (Uncharacterized protein OS=Schistosoma margrebowiei OX=48269 GN=SMRZ_LOCUS406 PE=4 SV=1)

HSP 1 Score: 129.028 bits (323), Expect = 3.362e-29
Identity = 97/250 (38.80%), Postives = 156/250 (62.40%), Query Frame = 3
Query:   63 KMSSGNSAVNALKSKMQNLKTEIDELKSKIEEKDKLLQEEIHKKSSIDQEIETLNKKIRELESQTDELDEKLSLSSIELDEVSKRADINERTRRGLESRKQNNDSKICKLESQLQEXXXXXXXXXXXYDEISKALKDSEMKLAEAEDRSEAATKRLKETDSEMHGVSHTLKSMELNESKATLREENYEKQINKITLQLKDAQTRYEIQESDYKRXXXXXXXXXXXXXXXXXXHYKLKNDLEKMMEEFQNI 812
            +MSS    + A+KSKMQ +K +ID+LK ++  K  +L++E   K+  + E+ +L K+IR+LE + +  + +L  ++++L+E SK AD ++R R+ LE+R   ++ +I +LE QL+E+  +   A++KYDE ++ L  +E+ L+ AEDR EAA  RLKE  S +HG    LKS+E  ES+A  REE YE+ I  +T +LK A+ R +  E          + LEE +E E+ NH  L+ ++E M  E +NI
Sbjct:  291 RMSS--PVIGAVKSKMQGMKLQIDQLKQELSTKQAILRKEEENKTKAEAEVASLQKRIRQLEDELESTETRLQEATLKLEEASKAADESDRGRKVLENRTFADEERINQLEEQLKESTFMAEDADRKYDEATRKLAVAEVALSHAEDRIEAAESRLKELQSIIHGTMGQLKSLEHQESQAAQREEAYEENIRDLTERLKAAEDRAQESERLVNTLQADADRLEETLEAEQLNHTNLRREMETMFTEVENI 538          
BLAST of SMED30023201 vs. TrEMBL
Match: Q5C3A9 (SJCHGC02288 protein (Fragment) OS=Schistosoma japonicum OX=6182 PE=2 SV=2)

HSP 1 Score: 118.242 bits (295), Expect = 1.383e-27
Identity = 77/204 (37.75%), Postives = 127/204 (62.25%), Query Frame = 3
Query:   66 MSSGNSAVNALKSKMQNLKTEIDELKSKIEEKDKLLQEEIHKKSSIDQEIETLNKKIRELESQTDELDEKLSLSSIELDEVSKRADINERTRRGLESRKQNNDSKICKLESQLQEXXXXXXXXXXXYDEISKALKDSEMKLAEAEDRSEAATKRLKETDSEMHGVSHTLKSMELNESKATLREENYEKQINKITLQLKDAQTRY 677
            MSS    + A+KSKMQ +K +ID+LK ++  K  +L++E   K+  + E+ +L K+IR+LE + +  + +L  ++++L+E SK AD ++R RR LE+R+   D +I +LES +QE       A  KY+E ++ L  +E+ L+ AEDR EAA  RLKE  S +HG    LKS+E  ES+ + +   ++ Q+  ++ QL +A+ R 
Sbjct:    1 MSS--PVIGAVKSKMQGMKLQIDQLKQEVSSKQAVLRKEEENKTKAEAEVASLQKRIRQLEDELESTETRLQEATLKLEEASKAADESDRARRVLEARQTAEDERILQLESMVQETAKSVKDAETKYEEATRKLAVAEVALSHAEDRIEAAESRLKELQSIIHGTMGQLKSLEHQESQLSKQRSLHQSQLASLSKQLIEAERRV 202          
BLAST of SMED30023201 vs. TrEMBL
Match: C7TXT8 (Tropomyosin-2 OS=Schistosoma japonicum OX=6182 GN=TMII PE=2 SV=1)

HSP 1 Score: 118.242 bits (295), Expect = 3.374e-27
Identity = 77/204 (37.75%), Postives = 127/204 (62.25%), Query Frame = 3
Query:   66 MSSGNSAVNALKSKMQNLKTEIDELKSKIEEKDKLLQEEIHKKSSIDQEIETLNKKIRELESQTDELDEKLSLSSIELDEVSKRADINERTRRGLESRKQNNDSKICKLESQLQEXXXXXXXXXXXYDEISKALKDSEMKLAEAEDRSEAATKRLKETDSEMHGVSHTLKSMELNESKATLREENYEKQINKITLQLKDAQTRY 677
            MSS    + A+KSKMQ +K +ID+LK ++  K  +L++E   K+  + E+ +L K+IR+LE + +  + +L  ++++L+E SK AD ++R RR LE+R+   D +I +LES +QE       A  KY+E ++ L  +E+ L+ AEDR EAA  RLKE  S +HG    LKS+E  ES+ + +   ++ Q+  ++ QL +A+ R 
Sbjct:    1 MSS--PVIGAVKSKMQGMKLQIDQLKQEVSSKQAVLRKEEENKTKAEAEVASLQKRIRQLEDELESTETRLQEATLKLEEASKAADESDRARRVLEARQTAEDERILQLESMVQETAKSVKDAETKYEEATRKLAVAEVALSHAEDRIEAAESRLKELQSIIHGTMGQLKSLEHQESQLSKQRSLHQSQLASLSKQLIEAERRV 202          
BLAST of SMED30023201 vs. TrEMBL
Match: A0A183JDQ8 (Uncharacterized protein OS=Schistosoma curassoni OX=6186 GN=SCUD_LOCUS821 PE=4 SV=1)

HSP 1 Score: 119.783 bits (299), Expect = 3.104e-26
Identity = 80/199 (40.20%), Postives = 130/199 (65.33%), Query Frame = 3
Query:   63 KMSSGNSAVNALKSKMQNLKTEIDELKSKIEEKDKLLQEEIHKKSSIDQEIETLNKKIRELESQTDELDEKLSLSSIELDEVSKRADINERTRRGLESRKQNNDSKICKLESQLQEXXXXXXXXXXXYDEISKALKDSEMKLAEAEDRSEAATKRLKETDSEMHGVSHTLKSMELNESKATLREENYEKQINKITLQLK 659
            +MSS    + A+KSKMQ +K +ID+LK ++  K  +L++E   K+  + E+ +L K+IR+LE + +  + +L  ++++L+E SK AD ++R R+ LE+R   ++ +I +LE QL+E+  +   A++KYDE ++ L  +E++L  AE R EAA  RLKE  S +HG    LKS+E  ES+A  REE YE+ I  +T +LK
Sbjct:  251 RMSS--PVIGAVKSKMQGMKLQIDQLKQELSTKQAILRKEEENKTKAEAEVASLQKRIRQLEDELESTETRLQEATVKLEEASKAADESDRGRKVLENRTFADEERINQLEEQLKESTFMAEDADRKYDEAARKLAITEVELERAESRLEAAESRLKELQSIIHGTMGQLKSLEHQESQAAQREEAYEENIRDLTERLK 447          
BLAST of SMED30023201 vs. Ensembl Cavefish
Match: tpm2 (tropomyosin 2 (beta) [Source:ZFIN;Acc:ZDB-GENE-040625-114])

HSP 1 Score: 72.7886 bits (177), Expect = 3.924e-14
Identity = 59/199 (29.65%), Postives = 121/199 (60.80%), Query Frame = 3
Query:   93 ALKSKMQNLKTEIDELKSKIEEKDKLLQEEIHKKSSIDQEIETLNKKIRELESQTDELDEKLSLSSIELDEVSKRADINERTRRGLESRKQNNDSKICKLESQLQEXXXXXXXXXXXYDEISKALKDSEMKLAEAEDRSEAATKRLKETDSEMHGVSHTLKSMELNESKATLREENYEKQINKITLQLKDAQTRYEIQE 689
             L+ K++ ++ E+D+    +++  + L++   K +  + E+ +LN++I+ +E + D   E+L+ +  +L+E  K AD +ER  + +E+R   ++ K+   E QL+EA ++   A++KY+E+++ L   E  L  +E+R+E A  +  + + E+  V++ LKS+E    K + +E+ YE++I  +T +LK+A+TR E  E
Sbjct:   45 GLQKKLKGVEDELDKYSESLKDAQEKLEQAEKKATDAEAEVASLNRRIQLVEEELDRAQERLATALQKLEEAEKAADESERGMKVIENRATKDEEKMELQEMQLKEAKHIAEEADRKYEEVARKLVILEGDLERSEERAEVAEAKSGDLEEELKNVTNNLKSLEAQAEKYSTKEDKYEEEIKVLTEKLKEAETRAEFAE 243          
BLAST of SMED30023201 vs. Ensembl Cavefish
Match: ENSAMXT00000052462.1 (tropomyosin alpha-4 chain-like [Source:NCBI gene;Acc:103045363])

HSP 1 Score: 72.0182 bits (175), Expect = 4.608e-14
Identity = 66/206 (32.04%), Postives = 126/206 (61.17%), Query Frame = 3
Query:   72 SGNSAVNALKSKMQNLKTEIDELKSKIEEKDKLLQEEIHKKSSIDQEIETLNKKIRELESQTDELDEKLSLSSIELDEVSKRADINERTRRGLESRKQNNDSKICKLESQLQEXXXXXXXXXXXYDEISKALKDSEMKLAEAEDRSEAATKRLKETDSEMHGVSHTLKSMELNESKATLREENYEKQINKITLQLKDAQTRYEIQE 689
            +G ++V A+K K++ L+ + DE + + E   + ++EE   +   + E+ +LN++I+ +E + D   E+L+ +  +L+E  K AD +ER  + +E+R   ++ K+   E QL+EA ++   A++KY+E+++ L   E  L  AE+R+E A  +  E + E+  V++ LKS+E    K + +E+ YE++I  +T +LK+A+TR E  E
Sbjct:    2 AGVNSVEAVKRKIKVLQQQADEAEERAEVLQRQVEEEKRAREQAEAEVASLNRRIQLVEEELDRAQERLTTALQKLEEAEKAADESERGMKVIENRALKDEEKMELQEVQLKEAKHIAEEADRKYEEVARKLVIIEGDLERAEERAEHAEAKCAELEEELKNVTNNLKSLEAQTEKYSQKEDKYEEEIKILTDKLKEAETRAEFAE 207          
BLAST of SMED30023201 vs. Ensembl Cavefish
Match: ENSAMXT00000004687.2 (tropomyosin alpha-4 chain-like [Source:NCBI gene;Acc:103045363])

HSP 1 Score: 72.0182 bits (175), Expect = 4.750e-14
Identity = 66/206 (32.04%), Postives = 126/206 (61.17%), Query Frame = 3
Query:   72 SGNSAVNALKSKMQNLKTEIDELKSKIEEKDKLLQEEIHKKSSIDQEIETLNKKIRELESQTDELDEKLSLSSIELDEVSKRADINERTRRGLESRKQNNDSKICKLESQLQEXXXXXXXXXXXYDEISKALKDSEMKLAEAEDRSEAATKRLKETDSEMHGVSHTLKSMELNESKATLREENYEKQINKITLQLKDAQTRYEIQE 689
            +G ++V A+K K++ L+ + DE + + E   + ++EE   +   + E+ +LN++I+ +E + D   E+L+ +  +L+E  K AD +ER  + +E+R   ++ K+   E QL+EA ++   A++KY+E+++ L   E  L  AE+R+E A  +  E + E+  V++ LKS+E    K + +E+ YE++I  +T +LK+A+TR E  E
Sbjct:    2 AGVNSVEAVKRKIKVLQQQADEAEERAEVLQRQVEEEKRAREQAEAEVASLNRRIQLVEEELDRAQERLTTALQKLEEAEKAADESERGMKVIENRALKDEEKMELQEVQLKEAKHIAEEADRKYEEVARKLVIIEGDLERAEERAEHAEAKCAELEEELKNVTNNLKSLEAQTEKYSQKEDKYEEEIKILTDKLKEAETRAEFAE 207          
BLAST of SMED30023201 vs. Ensembl Cavefish
Match: ENSAMXT00000037863.1 (tropomyosin alpha-4 chain-like [Source:NCBI gene;Acc:103045363])

HSP 1 Score: 72.0182 bits (175), Expect = 5.773e-14
Identity = 66/206 (32.04%), Postives = 126/206 (61.17%), Query Frame = 3
Query:   72 SGNSAVNALKSKMQNLKTEIDELKSKIEEKDKLLQEEIHKKSSIDQEIETLNKKIRELESQTDELDEKLSLSSIELDEVSKRADINERTRRGLESRKQNNDSKICKLESQLQEXXXXXXXXXXXYDEISKALKDSEMKLAEAEDRSEAATKRLKETDSEMHGVSHTLKSMELNESKATLREENYEKQINKITLQLKDAQTRYEIQE 689
            +G ++V A+K K++ L+ + DE + + E   + ++EE   +   + E+ +LN++I+ +E + D   E+L+ +  +L+E  K AD +ER  + +E+R   ++ K+   E QL+EA ++   A++KY+E+++ L   E  L  AE+R+E A  +  E + E+  V++ LKS+E    K + +E+ YE++I  +T +LK+A+TR E  E
Sbjct:    2 AGVNSVEAVKRKIKVLQQQADEAEERAEVLQRQVEEEKRAREQAEAEVASLNRRIQLVEEELDRAQERLTTALQKLEEAEKAADESERGMKVIENRALKDEEKMELQEVQLKEAKHIAEEADRKYEEVARKLVIIEGDLERAEERAEHAEAKCAELEEELKNVTNNLKSLEAQTEKYSQKEDKYEEEIKILTDKLKEAETRAEFAE 207          
BLAST of SMED30023201 vs. Ensembl Cavefish
Match: tpm4b (tropomyosin alpha-1 chain-like [Source:NCBI gene;Acc:103021908])

HSP 1 Score: 72.0182 bits (175), Expect = 8.414e-14
Identity = 54/167 (32.34%), Postives = 103/167 (61.68%), Query Frame = 3
Query:  189 KKSSIDQEIETLNKKIRELESQTDELDEKLSLSSIELDEVSKRADINERTRRGLESRKQNNDSKICKLESQLQEXXXXXXXXXXXYDEISKALKDSEMKLAEAEDRSEAATKRLKETDSEMHGVSHTLKSMELNESKATLREENYEKQINKITLQLKDAQTRYEIQE 689
            K +  + E+  LN++I+ +E + D   E+L+ +  +L+E  K+AD +ER  + +E+R   ++ K+   E QL+EA  +   +++KYDE+++ L   E +L +AE+R+E +  +  + + E+  V++TLKS+E    K   +E+ YE++I  +T +LK+ +TR E  E
Sbjct:   77 KATEAEGEVAALNRRIQLIEEELDRAQERLATALQKLEEAEKQADESERGMKVIENRATKDEEKMVMQELQLKEAKQVAEDSDRKYDEVARKLVILEGELEKAEERAEISELKGGDLEEELKNVTNTLKSLEAQSEKYADKEDRYEEEIKVLTDRLKETETRAEFAE 243          
BLAST of SMED30023201 vs. Ensembl Sea Lamprey
Match: tpm3 (tropomyosin 3 [Source:ZFIN;Acc:ZDB-GENE-030826-16])

HSP 1 Score: 68.5514 bits (166), Expect = 8.955e-14
Identity = 53/159 (33.33%), Postives = 98/159 (61.64%), Query Frame = 3
Query:  213 IETLNKKIRELESQTDELDEKLSLSSIELDEVSKRADINERTRRGLESRKQNNDSKICKLESQLQEXXXXXXXXXXXYDEISKALKDSEMKLAEAEDRSEAATKRLKETDSEMHGVSHTLKSMELNESKATLREENYEKQINKITLQLKDAQTRYEIQE 689
            + +LN++I+ +E + D   E+LS +  +L+E  K AD +ER  + +E+R   ++ K+   E QL+EA ++   +++KY+E+++ L   E  L   E+R+E A  +  E + E+  V++ LKS+E    K + +E+ YE++I  +T +LK+A+TR E  E
Sbjct:    6 VASLNRRIQLVEEELDRAQERLSTALQKLEEAEKAADESERGMKVIENRASKDEEKMELQEIQLKEAKHIAEESDRKYEEVARKLVILEGDLERTEERAEVADAKCSELEEELKVVTNNLKSLEAQSDKYSQKEDKYEEEIKILTDKLKEAETRAEFAE 164          
BLAST of SMED30023201 vs. Ensembl Sea Lamprey
Match: TPM4 (tropomyosin 4 [Source:HGNC Symbol;Acc:HGNC:12013])

HSP 1 Score: 64.6994 bits (156), Expect = 1.909e-12
Identity = 49/159 (30.82%), Postives = 100/159 (62.89%), Query Frame = 3
Query:  213 IETLNKKIRELESQTDELDEKLSLSSIELDEVSKRADINERTRRGLESRKQNNDSKICKLESQLQEXXXXXXXXXXXYDEISKALKDSEMKLAEAEDRSEAATKRLKETDSEMHGVSHTLKSMELNESKATLREENYEKQINKITLQLKDAQTRYEIQE 689
            + +LN++I+ +E + D   E+L+ +  +L+E +K AD +ER  + +E+R   ++ K+   E QL+EA ++   A++KY+E+++ L   E +L   E+R+E    R+++ + E+  +   +KS+ + E + + +E+ YE++I  +T +LK+A+TR E  E
Sbjct:    7 VASLNRRIQLVEEELDRAQERLATALQKLEEATKAADESERGMKVIENRALKDEEKMELQEIQLKEAKHIAEEADRKYEEVARKLVIIEGELERTEERAEVGETRVRQLEEELRVMDQNMKSLLVAEEQYSQKEDKYEEEIKILTDKLKEAETRAEFAE 165          
BLAST of SMED30023201 vs. Ensembl Sea Lamprey
Match: TPM4 (tropomyosin 4 [Source:HGNC Symbol;Acc:HGNC:12013])

HSP 1 Score: 62.3882 bits (150), Expect = 6.179e-12
Identity = 49/159 (30.82%), Postives = 99/159 (62.26%), Query Frame = 3
Query:  213 IETLNKKIRELESQTDELDEKLSLSSIELDEVSKRADINERTRRGLESRKQNNDSKICKLESQLQEXXXXXXXXXXXYDEISKALKDSEMKLAEAEDRSEAATKRLKETDSEMHGVSHTLKSMELNESKATLREENYEKQINKITLQLKDAQTRYEIQE 689
            + +LN++I+ +E + D   E+L+ +  +L++  K AD +ER  + +E+R   ++ K+   E QL+EA ++   A++KY+E+++ L   E  L   E+R+E +  R+++ + E+  V   L+S+  +E + + +E+ YE++I  +T +LK+A+TR E  E
Sbjct:    6 VASLNRRIQLVEEELDRAQERLTTALQKLEDAEKAADESERGMKVIENRAGKDEEKMELQEIQLKEAKHIAEEADRKYEEVARKLVIIEGDLERTEERAEISETRVRQMEDELRHVQEELRSLFASEEQYSQKEDKYEEEIKVLTDKLKEAETRAEFAE 164          
BLAST of SMED30023201 vs. Ensembl Nematostella
Match: EDO37230 (Predicted protein; Tropomyosin [Source:UniProtKB/TrEMBL;Acc:Q6S5G5])

HSP 1 Score: 80.4925 bits (197), Expect = 2.182e-17
Identity = 66/195 (33.85%), Postives = 122/195 (62.56%), Query Frame = 3
Query:   96 LKSKMQNLKTEIDELKSKIEEKDKLLQEEIHKKSSIDQEIETLNKKIRELESQTDELDEKLSLSSIELDEVSKRADINERTRRGLESRKQNNDSKICKLESQLQEXXXXXXXXXXXYDEISKALKDSEMKLAEAEDRSEAATKRLKETDSEMHGVSHTLKSMELNESKATLREENYEKQINKITLQLKDAQTRYE 680
            +K KM  L+  +++ +++  + +  L+    +  S + E+  L K++++LE   D  + KL+ +  +L E  K+AD +ER R+ LE+R  +++ ++  LE Q  +AL     A K+Y+EIS+ L++ E +L EAE +++AA  R+KE + E+  V + L+S+E++E KA+ RE+ YE QI ++  +L+DA+ R E
Sbjct:    4 IKKKMATLRQTLEDAEARAAKAEDELKNANDRADSAETEVAALTKQLQQLEDDLDAAESKLADTQGQLTEAEKQADESERARKVLENRGASDEERLASLERQYNDALERTEEAEKQYEEISERLQELENELEEAEQKADAAEARVKELEEEVTLVGNNLRSLEISEGKASEREDTYENQIRELETKLQDAEERAE 198          
BLAST of SMED30023201 vs. Ensembl Medaka
Match: ENSORLT00000024131.2 (tropomyosin alpha-3 chain [Source:NCBI gene;Acc:101168689])

HSP 1 Score: 87.4261 bits (215), Expect = 2.061e-19
Identity = 63/208 (30.29%), Postives = 129/208 (62.02%), Query Frame = 3
Query:   66 MSSGNSAVNALKSKMQNLKTEIDELKSKIEEKDKLLQEEIHKKSSIDQEIETLNKKIRELESQTDELDEKLSLSSIELDEVSKRADINERTRRGLESRKQNNDSKICKLESQLQEXXXXXXXXXXXYDEISKALKDSEMKLAEAEDRSEAATKRLKETDSEMHGVSHTLKSMELNESKATLREENYEKQINKITLQLKDAQTRYEIQE 689
            MS G +++ A+K K++ L+ + +E   + E+  + +++E   +   + E+ +L ++++  E   D + E+L  + ++LDEV K AD +ER  + +E+R   ++ K+  LE QL+EA  +   A++KY+E+++ L   E +L  AEDR+E A  +++  + ++     +LKS++ +E K +L+E+ YE++I  ++ +LK+A+ R E+ E
Sbjct:    1 MSGGMNSIEAVKKKIKVLQDQAEEADERAEKLQREVEKEKRSREEAEAEVSSLGRRLQLSEENLDRVHERLGAALLKLDEVEKVADESERGMKVIENRALKDEEKMELLEVQLREAKQIAEEADRKYEEVARKLVIVEGELERAEDRAEQADSKVRRLEEQLRSYDQSLKSLQASEDKYSLKEDRYEEEIKNLSNKLKEAEARAELSE 208          
BLAST of SMED30023201 vs. Ensembl Medaka
Match: TPM1 (tropomyosin 1 [Source:NCBI gene;Acc:101168205])

HSP 1 Score: 80.8777 bits (198), Expect = 4.159e-17
Identity = 65/206 (31.55%), Postives = 125/206 (60.68%), Query Frame = 3
Query:   72 SGNSAVNALKSKMQNLKTEIDELKSKIEEKDKLLQEEIHKKSSIDQEIETLNKKIRELESQTDELDEKLSLSSIELDEVSKRADINERTRRGLESRKQNNDSKICKLESQLQEXXXXXXXXXXXYDEISKALKDSEMKLAEAEDRSEAATKRLKETDSEMHGVSHTLKSMELNESKATLREENYEKQINKITLQLKDAQTRYEIQE 689
            +G S+V+A+K K+++L+ + D  + + E   K L E    +   + ++ +LN++I+ +E + D   E+L+ +  +L+E  K AD +ER  + +E+R   ++ K+   E QL+EA ++   A++KY+E+++ L   E  L   E+R+E +  +  E + E+  V++ LKS+E    K +L+E+ YE++I  +T +LK+A+TR E  E
Sbjct:    2 AGVSSVDAVKRKIRSLQEQADGAEERAERLQKELLEYRKAREHAESDVASLNRRIQLVEEELDRAQERLATALTKLEEAEKAADESERGMKVIENRAMKDEEKMEIQEIQLKEAKHIAEEADRKYEEVARKLVIIESDLERTEERAELSEGKCSELEEELKTVTNNLKSLEAQAEKYSLKEDKYEEEIKVLTDKLKEAETRAEFAE 207          
BLAST of SMED30023201 vs. Ensembl Medaka
Match: tpm4a (tropomyosin 4 [Source:NCBI gene;Acc:101160801])

HSP 1 Score: 78.1814 bits (191), Expect = 3.889e-16
Identity = 64/206 (31.07%), Postives = 124/206 (60.19%), Query Frame = 3
Query:   72 SGNSAVNALKSKMQNLKTEIDELKSKIEEKDKLLQEEIHKKSSIDQEIETLNKKIRELESQTDELDEKLSLSSIELDEVSKRADINERTRRGLESRKQNNDSKICKLESQLQEXXXXXXXXXXXYDEISKALKDSEMKLAEAEDRSEAATKRLKETDSEMHGVSHTLKSMELNESKATLREENYEKQINKITLQLKDAQTRYEIQE 689
            +G ++++A+K K+Q L+ + DE + + E   K L  E   +   + ++  LN++I+ +E + D   E+L+ +  +L+E  K AD +ER  + +E+R   ++ K+   E QL+EA ++   A++KY+E+++ L   E  L  AE+R+E +  +  + + E+  V++ LKS+E    K + +E+ YE++I  +T +LK+A+TR E  E
Sbjct:    2 AGMNSLDAVKRKIQCLQQQADEAEDRAESLQKELDNERELREKAEGDVAALNRRIQLVEEELDRAQERLATALQKLEEAEKAADESERGMKVIENRAMKDEEKMEIQEMQLKEAKHIAEEADRKYEEVARKLVILEGDLERAEERAEVSESKASDLEEELKNVTNNLKSLEAQAEKYSEKEDKYEEEIKVLTDKLKEAETRAEFAE 207          
BLAST of SMED30023201 vs. Ensembl Medaka
Match: ENSORLT00000046199.1 (tropomyosin alpha-1 chain [Source:NCBI gene;Acc:101164789])

HSP 1 Score: 76.6406 bits (187), Expect = 1.122e-15
Identity = 61/206 (29.61%), Postives = 124/206 (60.19%), Query Frame = 3
Query:   72 SGNSAVNALKSKMQNLKTEIDELKSKIEEKDKLLQEEIHKKSSIDQEIETLNKKIRELESQTDELDEKLSLSSIELDEVSKRADINERTRRGLESRKQNNDSKICKLESQLQEXXXXXXXXXXXYDEISKALKDSEMKLAEAEDRSEAATKRLKETDSEMHGVSHTLKSMELNESKATLREENYEKQINKITLQLKDAQTRYEIQE 689
            +G S++ A+K K+++L+ + D  + +  +  + L +E   + + + ++ +LN++I+ +E + D   E+L+ +  +L+E  K AD +ER  + +E+R   ++ K+   E QL+EA ++   A++KY+E+++ L   E  L   E+R+E +  +  E + E+  V + LKS+E    K + +E+ YE++I  +T +LK+A+TR E  E
Sbjct:    2 AGGSSLEAVKKKIKSLQEQADAAEERAAQLQRDLNQERSAREAAEGDVASLNRRIQLVEEELDRAQERLATALTKLEEAEKAADESERGMKVIENRAMKDEEKMELQEIQLKEAKHIAEEADRKYEEVARKLVIIESDLERTEERAELSEGKCSELEEELKTVQNNLKSLEAQAEKYSQKEDKYEEEIKVLTDKLKEAETRAEFAE 207          
BLAST of SMED30023201 vs. Ensembl Medaka
Match: ENSORLT00000015430.2 (tropomyosin alpha-1 chain [Source:NCBI gene;Acc:101164789])

HSP 1 Score: 76.2554 bits (186), Expect = 1.636e-15
Identity = 61/206 (29.61%), Postives = 124/206 (60.19%), Query Frame = 3
Query:   72 SGNSAVNALKSKMQNLKTEIDELKSKIEEKDKLLQEEIHKKSSIDQEIETLNKKIRELESQTDELDEKLSLSSIELDEVSKRADINERTRRGLESRKQNNDSKICKLESQLQEXXXXXXXXXXXYDEISKALKDSEMKLAEAEDRSEAATKRLKETDSEMHGVSHTLKSMELNESKATLREENYEKQINKITLQLKDAQTRYEIQE 689
            +G S++ A+K K+++L+ + D  + +  +  + L +E   + + + ++ +LN++I+ +E + D   E+L+ +  +L+E  K AD +ER  + +E+R   ++ K+   E QL+EA ++   A++KY+E+++ L   E  L   E+R+E +  +  E + E+  V + LKS+E    K + +E+ YE++I  +T +LK+A+TR E  E
Sbjct:    2 AGGSSLEAVKKKIKSLQEQADAAEERAAQLQRDLNQERSAREAAEGDVASLNRRIQLVEEELDRAQERLATALTKLEEAEKAADESERGMKVIENRAMKDEEKMELQEIQLKEAKHIAEEADRKYEEVARKLVIIESDLERTEERAELSEGKCSELEEELKTVQNNLKSLEAQAEKYSQKEDKYEEEIKVLTDKLKEAETRAEFAE 207          
BLAST of SMED30023201 vs. Planmine SMEST
Match: SMESG000041543.1 (SMESG000041543.1)

HSP 1 Score: 416.001 bits (1068), Expect = 1.086e-146
Identity = 249/249 (100.00%), Postives = 249/249 (100.00%), Query Frame = 3
Query:   66 MSSGNSAVNALKSKMQNLKTEIDELKSKIEEKDKLLQEEIHKKSSIDQEIETLNKKIRELESQTDELDEKLSLSSIELDEVSKRADINERTRRGLESRKQNNDSKICKLESQLQEXXXXXXXXXXXYDEISKALKDSEMKLAEAEDRSEAATKRLKETDSEMHGVSHTLKSMELNESKATLREENYEKQINKITLQLKDAQTRYEIQESDYKRXXXXXXXXXXXXXXXXXXHYKLKNDLEKMMEEFQNI 812
            MSSGNSAVNALKSKMQNLKTEIDELKSKIEEKDKLLQEEIHKKSSIDQEIETLNKKIRELESQTDELDEKLSLSSIELDEVSKRADINERTRRGLESRKQNNDSKICKLESQLQEALNLKAAANKKYDEISKALKDSEMKLAEAEDRSEAATKRLKETDSEMHGVSHTLKSMELNESKATLREENYEKQINKITLQLKDAQTRYEIQESDYKRQVTLLENLEEQVEQEKENHYKLKNDLEKMMEEFQNI
Sbjct:    1 MSSGNSAVNALKSKMQNLKTEIDELKSKIEEKDKLLQEEIHKKSSIDQEIETLNKKIRELESQTDELDEKLSLSSIELDEVSKRADINERTRRGLESRKQNNDSKICKLESQLQEALNLKAAANKKYDEISKALKDSEMKLAEAEDRSEAATKRLKETDSEMHGVSHTLKSMELNESKATLREENYEKQINKITLQLKDAQTRYEIQESDYKRQVTLLENLEEQVEQEKENHYKLKNDLEKMMEEFQNI 249          
BLAST of SMED30023201 vs. Planmine SMEST
Match: SMESG000041543.1 (SMESG000041543.1)

HSP 1 Score: 263.848 bits (673), Expect = 6.002e-88
Identity = 152/152 (100.00%), Postives = 152/152 (100.00%), Query Frame = 3
Query:   66 MSSGNSAVNALKSKMQNLKTEIDELKSKIEEKDKLLQEEIHKKSSIDQEIETLNKKIRELESQTDELDEKLSLSSIELDEVSKRADINERTRRGLESRKQNNDSKICKLESQLQEXXXXXXXXXXXYDEISKALKDSEMKLAEAEDRSEAAT 521
            MSSGNSAVNALKSKMQNLKTEIDELKSKIEEKDKLLQEEIHKKSSIDQEIETLNKKIRELESQTDELDEKLSLSSIELDEVSKRADINERTRRGLESRKQNNDSKICKLESQLQEALNLKAAANKKYDEISKALKDSEMKLAEAEDRSEAAT
Sbjct:    1 MSSGNSAVNALKSKMQNLKTEIDELKSKIEEKDKLLQEEIHKKSSIDQEIETLNKKIRELESQTDELDEKLSLSSIELDEVSKRADINERTRRGLESRKQNNDSKICKLESQLQEALNLKAAANKKYDEISKALKDSEMKLAEAEDRSEAAT 152          
BLAST of SMED30023201 vs. Planmine SMEST
Match: SMESG000041543.1 (SMESG000041543.1)

HSP 1 Score: 137.887 bits (346), Expect = 3.187e-40
Identity = 88/88 (100.00%), Postives = 88/88 (100.00%), Query Frame = 3
Query:  549 MHGVSHTLKSMELNESKATLREENYEKQINKITLQLKDAQTRYEIQESDYKRXXXXXXXXXXXXXXXXXXHYKLKNDLEKMMEEFQNI 812
            MHGVSHTLKSMELNESKATLREENYEKQINKITLQLKDAQTRYEIQESDYKRQVTLLENLEEQVEQEKENHYKLKNDLEKMMEEFQNI
Sbjct:    1 MHGVSHTLKSMELNESKATLREENYEKQINKITLQLKDAQTRYEIQESDYKRQVTLLENLEEQVEQEKENHYKLKNDLEKMMEEFQNI 88          
BLAST of SMED30023201 vs. Planmine SMEST
Match: SMESG000024190.1 (SMESG000024190.1)

HSP 1 Score: 102.449 bits (254), Expect = 5.332e-25
Identity = 82/249 (32.93%), Postives = 149/249 (59.84%), Query Frame = 3
Query:   66 MSSGNSAVNALKSKMQNLKTEIDELKSKIEEKDKLLQEEIHKKSSIDQEIETLNKKIRELESQTDELDEKLSLSSIELDEVSKRADINERTRRGLESRKQNNDSKICKLESQLQEXXXXXXXXXXXYDEISKALKDSEMKLAEAEDRSEAATKRLKETDSEMHGVSHTLKSMELNESKATLREENYEKQINKITLQLKDAQTRYEIQESDYKRXXXXXXXXXXXXXXXXXXHYKLKNDLEKMMEEFQNI 812
            M+     V   K K++ L+ EI+ L+ ++ EK+  L+EE H +  +D+++  L++K+  LES  D+ +++L  +  +L+E++  A   ER RR L+S+   +D +I  LE+ ++EA      A  +Y+E+S+ L  +E  L   ED  E   K+L+E   EM  V ++ + +E+NE++A  RE+ +E ++  +   L+DA  R    E++  ++  +L+ LEE +++EK NH  L NDL++M+EEFQ +
Sbjct:    1 MNETVELVIVFKKKIEELRKEIEVLEVQLHEKETKLKEEQHTRDELDKQVVLLSRKVGVLESDLDDNEKRLQEAKFKLEEITFTAAECERARRQLQSKHDADDERIYHLETLVKEATEQAGEAEARYEEVSRKLIATENVLENTEDEYEKTEKKLREITMEMTSVCNSFRVLEINEARAAEREQKFEVEVKHLKKLLQDADKRANEAENELNQRQMMLDQLEEALDKEKRNHQLLSNDLQRMVEEFQQM 249          
BLAST of SMED30023201 vs. Planmine SMEST
Match: SMESG000011341.1 (SMESG000011341.1)

HSP 1 Score: 93.5893 bits (231), Expect = 1.150e-21
Identity = 84/247 (34.01%), Postives = 155/247 (62.75%), Query Frame = 3
Query:   72 SGNSAVNALKSKMQNLKTEIDELKSKIEEKDKLLQEEIHKKSSIDQEIETLNKKIRELESQTDELDEKLSLSSIELDEVSKRADINERTRRGLESRKQNNDSKICKLESQLQEXXXXXXXXXXXYDEISKALKDSEMKLAEAEDRSEAATKRLKETDSEMHGVSHTLKSMELNESKATLREENYEKQINKITLQLKDAQTRYEIQESDYKRXXXXXXXXXXXXXXXXXXHYKLKNDLEKMMEEFQNI 812
            +G  A   +K++M+ L  EI++L+ +I  +DKL+  E  K S +  E + L KK+  ++ +  + + KL   S  L+++SK A  +ER R+ +E+R+  +  ++ +LE  ++ +L     AN+K +E+SK L  SE  L+EAE+RS  A  R+KE ++++  V+  LKS+E+ + KA  RE NY+ +I  +TLQ+K+A+ R    E D +++   L+ ++E++++EK N+Y +K +L+KM  E+ ++
Sbjct:    4 NGPPAALVIKARMKTLNNEIEKLQDEILSRDKLIDIEKEKNSEVKLESDNLKKKLAIIDQEKLDFETKLKEVSQSLEKISKSAHKSERERKVMENRQNADSDRMDQLEEAIKLSLIATDEANRKSEELSKKLIQSEHALSEAENRSAFAENRIKELEADLKSVASKLKSLEIKKDKAVEREVNYDAKIRCLTLQVKEAEERAIAAEQDVRKREFELDKVKEEIDREKANYYHIKKELDKMHAEYFSV 250          
The following BLAST results are available for this feature:
BLAST of SMED30023201 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
TPM14.886e-1631.43tropomyosin 1 [Source:HGNC Symbol;Acc:HGNC:12010][more]
TPM15.324e-1631.43tropomyosin 1 [Source:HGNC Symbol;Acc:HGNC:12010][more]
TPM15.330e-1631.43tropomyosin 1 [Source:HGNC Symbol;Acc:HGNC:12010][more]
TPM16.531e-1631.43tropomyosin 1 [Source:HGNC Symbol;Acc:HGNC:12010][more]
TPM31.051e-1530.58tropomyosin 3 [Source:HGNC Symbol;Acc:HGNC:12012][more]
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BLAST of SMED30023201 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
lev-112.231e-2031.31Tropomyosin isoforms a/b/d/f [Source:UniProtKB/Sw... [more]
lev-113.443e-2034.10Tropomyosin isoforms a/b/d/f [Source:UniProtKB/Sw... [more]
lev-114.626e-2034.10Tropomyosin isoforms a/b/d/f [Source:UniProtKB/Sw... [more]
lev-114.626e-2034.10Tropomyosin isoforms a/b/d/f [Source:UniProtKB/Sw... [more]
lev-111.721e-1835.16Tropomyosin isoforms a/b/d/f [Source:UniProtKB/Sw... [more]
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BLAST of SMED30023201 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Tm11.063e-2235.57gene:FBgn0003721 transcript:FBtr0333917[more]
Tm11.796e-2034.54gene:FBgn0003721 transcript:FBtr0305656[more]
Tm14.271e-2034.54gene:FBgn0003721 transcript:FBtr0089960[more]
Tm11.037e-1934.87gene:FBgn0003721 transcript:FBtr0333918[more]
Tm14.648e-1935.38gene:FBgn0003721 transcript:FBtr0305654[more]
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BLAST of SMED30023201 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
tpm33.633e-1830.58tropomyosin 3 [Source:ZFIN;Acc:ZDB-GENE-030826-16][more]
tpm31.851e-1731.07tropomyosin 3 [Source:ZFIN;Acc:ZDB-GENE-030826-16][more]
tpm21.412e-1531.13tropomyosin 2 (beta) [Source:ZFIN;Acc:ZDB-GENE-040... [more]
tpma1.378e-1429.13alpha-tropomyosin [Source:NCBI gene;Acc:30324][more]
tpm37.628e-1433.72tropomyosin 3 [Source:ZFIN;Acc:ZDB-GENE-030826-16][more]
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BLAST of SMED30023201 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
tpm11.213e-1329.13tropomyosin 1 [Source:NCBI gene;Acc:448541][more]
tpm15.671e-1329.61tropomyosin 1 [Source:NCBI gene;Acc:448541][more]
tpm16.006e-1331.84tropomyosin 1 [Source:NCBI gene;Acc:448541][more]
tpm18.572e-1331.84tropomyosin 1 [Source:NCBI gene;Acc:448541][more]
tpm18.967e-1331.84tropomyosin 1 [Source:NCBI gene;Acc:448541][more]
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BLAST of SMED30023201 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Tpm32.400e-1630.58tropomyosin 3, gamma [Source:MGI Symbol;Acc:MGI:18... [more]
Tpm32.486e-1630.58tropomyosin 3, gamma [Source:MGI Symbol;Acc:MGI:18... [more]
Tpm15.123e-1630.95tropomyosin 1, alpha [Source:MGI Symbol;Acc:MGI:98... [more]
Tpm15.708e-1630.95tropomyosin 1, alpha [Source:MGI Symbol;Acc:MGI:98... [more]
Tpm36.785e-1630.10tropomyosin 3, gamma [Source:MGI Symbol;Acc:MGI:18... [more]
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BLAST of SMED30023201 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q8WR63|TPM_TRIPS8.145e-2335.38Tropomyosin OS=Trichinella pseudospiralis OX=6337 ... [more]
sp|Q95VA8|TPM_TRISP1.621e-2235.38Tropomyosin OS=Trichinella spiralis OX=6334 PE=2 S... [more]
sp|Q23939|TPM_DERFA5.128e-2134.85Tropomyosin OS=Dermatophagoides farinae OX=6954 PE... [more]
sp|P0DSM7|TPM02_PERAM1.815e-2035.20Tropomyosin Per a 7.0102 OS=Periplaneta americana ... [more]
sp|Q8T6L5|TPM_PERFU2.063e-2035.20Tropomyosin OS=Periplaneta fuliginosa OX=36977 PE=... [more]
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BLAST of SMED30023201 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
Q5C3B45.190e-3240.20SJCHGC02287 protein (Fragment) OS=Schistosoma japo... [more]
A0A183L9D13.362e-2938.80Uncharacterized protein OS=Schistosoma margrebowie... [more]
Q5C3A91.383e-2737.75SJCHGC02288 protein (Fragment) OS=Schistosoma japo... [more]
C7TXT83.374e-2737.75Tropomyosin-2 OS=Schistosoma japonicum OX=6182 GN=... [more]
A0A183JDQ83.104e-2640.20Uncharacterized protein OS=Schistosoma curassoni O... [more]
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BLAST of SMED30023201 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
tpm23.924e-1429.65tropomyosin 2 (beta) [Source:ZFIN;Acc:ZDB-GENE-040... [more]
ENSAMXT00000052462.14.608e-1432.04tropomyosin alpha-4 chain-like [Source:NCBI gene;A... [more]
ENSAMXT00000004687.24.750e-1432.04tropomyosin alpha-4 chain-like [Source:NCBI gene;A... [more]
ENSAMXT00000037863.15.773e-1432.04tropomyosin alpha-4 chain-like [Source:NCBI gene;A... [more]
tpm4b8.414e-1432.34tropomyosin alpha-1 chain-like [Source:NCBI gene;A... [more]
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BLAST of SMED30023201 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 3
Match NameE-valueIdentityDescription
tpm38.955e-1433.33tropomyosin 3 [Source:ZFIN;Acc:ZDB-GENE-030826-16][more]
TPM41.909e-1230.82tropomyosin 4 [Source:HGNC Symbol;Acc:HGNC:12013][more]
TPM46.179e-1230.82tropomyosin 4 [Source:HGNC Symbol;Acc:HGNC:12013][more]
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BLAST of SMED30023201 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023201 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO372302.182e-1733.85Predicted protein; Tropomyosin [Source:UniProtKB/... [more]
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BLAST of SMED30023201 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSORLT00000024131.22.061e-1930.29tropomyosin alpha-3 chain [Source:NCBI gene;Acc:10... [more]
TPM14.159e-1731.55tropomyosin 1 [Source:NCBI gene;Acc:101168205][more]
tpm4a3.889e-1631.07tropomyosin 4 [Source:NCBI gene;Acc:101160801][more]
ENSORLT00000046199.11.122e-1529.61tropomyosin alpha-1 chain [Source:NCBI gene;Acc:10... [more]
ENSORLT00000015430.21.636e-1529.61tropomyosin alpha-1 chain [Source:NCBI gene;Acc:10... [more]
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BLAST of SMED30023201 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000041543.11.086e-146100.00SMESG000041543.1[more]
SMESG000041543.16.002e-88100.00SMESG000041543.1[more]
SMESG000041543.13.187e-40100.00SMESG000041543.1[more]
SMESG000024190.15.332e-2532.93SMESG000024190.1[more]
SMESG000011341.11.150e-2134.01SMESG000011341.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30023201 ID=SMED30023201|Name=SMED30023201|organism=Schmidtea mediterranea sexual|type=transcript|length=1164bp
ATGACATTCATATTTAAGCCGCCAATTGATTTTTAGTTTAATTTTTTGTC
TTGATAATACGAAAAATGAGTTCCGGCAATAGTGCAGTAAATGCTTTGAA
AAGTAAAATGCAAAATCTCAAAACAGAGATAGATGAATTAAAATCCAAAA
TTGAAGAAAAAGACAAATTACTCCAAGAAGAAATACACAAAAAATCATCT
ATCGATCAAGAAATTGAAACATTGAATAAAAAAATCAGGGAGCTCGAATC
ACAAACTGATGAGTTGGATGAAAAATTGTCGCTGTCTTCAATCGAGTTAG
ACGAAGTTAGCAAACGTGCCGACATCAATGAAAGAACTCGCCGGGGATTG
GAAAGTAGAAAACAAAACAATGATAGTAAAATCTGTAAATTAGAAAGTCA
ATTACAAGAAGCATTAAATTTGAAAGCTGCCGCTAATAAAAAATATGATG
AAATATCGAAAGCTTTAAAAGATAGCGAAATGAAATTAGCTGAAGCCGAA
GACAGATCGGAAGCAGCTACTAAGAGATTAAAAGAAACAGATTCCGAAAT
GCATGGAGTATCTCATACATTGAAATCAATGGAATTAAATGAATCAAAAG
CAACATTAAGAGAAGAAAATTATGAAAAGCAAATTAACAAGATTACTTTG
CAATTGAAAGATGCTCAAACAAGATATGAAATCCAGGAATCTGATTACAA
ACGCCAAGTGACCTTGCTGGAAAACTTAGAAGAACAAGTTGAACAGGAAA
AAGAAAATCATTATAAATTGAAAAATGATTTAGAAAAAATGATGGAAGAA
TTTCAGAATATCTAAAATATTGATATTGCTTTTATTTACTATCGAAATCA
CTATTATTTTGTCTGATTTTATAGCATTTGTTAGTATTATTATATTTGTT
TTGCTCAAATGTTTATCACCATAGTATTGATTGTTTTATTGGTTTTCATT
TGATTTAATGTAAAAACAAACATTCCAGTGTAATTTTGTTTACCTAAGTG
ATTTTGAAATGCTATATCGATTAATAAATAAAAATCTCTCGCTATTTTAT
TACAAATAAGGCCGCATAATAATTATATAATTAATTGTGCGTTGTCCAAA
AATCTCTAGTGGTAATCTCATCATATTATGATTACTGCTAGCTTACAGTA
AATATTTGTTACTG
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protein sequence of SMED30023201-orf-1

>SMED30023201-orf-1 ID=SMED30023201-orf-1|Name=SMED30023201-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=250bp
MSSGNSAVNALKSKMQNLKTEIDELKSKIEEKDKLLQEEIHKKSSIDQEI
ETLNKKIRELESQTDELDEKLSLSSIELDEVSKRADINERTRRGLESRKQ
NNDSKICKLESQLQEALNLKAAANKKYDEISKALKDSEMKLAEAEDRSEA
ATKRLKETDSEMHGVSHTLKSMELNESKATLREENYEKQINKITLQLKDA
QTRYEIQESDYKRQVTLLENLEEQVEQEKENHYKLKNDLEKMMEEFQNI*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000034epidermis
PLANA:0000101muscle cell
PLANA:0000103central nervous system
PLANA:0000208testis
PLANA:0002075pigment cell
PLANA:0003001lateral region of the whole animal
PLANA:0003116parenchymal cell
Vocabulary: INTERPRO
TermDefinition
IPR000533Tropomyosin
Vocabulary: molecular function
TermDefinition
GO:0003779actin binding
GO:0008307structural constituent of muscle
GO:0051015actin filament binding
Vocabulary: cellular component
TermDefinition
GO:0005737cytoplasm
GO:0005862muscle thin filament tropomyosin
GO:0005884actin filament
Vocabulary: biological process
TermDefinition
GO:0006936muscle contraction
GO:0007015actin filament organization
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 113..161
NoneNo IPR availableCOILSCoilCoilcoord: 8..77
NoneNo IPR availableCOILSCoilCoilcoord: 183..245
NoneNo IPR availableGENE3DG3DSA:1.20.5.340coord: 6..104
e-value: 1.7E-8
score: 36.4
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 140..166
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 82..104
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 140..176
NoneNo IPR availablePANTHERPTHR19269:SF45TROPOMYOSIN-1, ISOFORMS 33/34coord: 5..247
NoneNo IPR availablePANTHERPTHR19269TROPOMYOSINcoord: 5..247
NoneNo IPR availableSUPERFAMILYSSF57997Tropomyosincoord: 5..248
IPR000533TropomyosinPRINTSPR00194TROPOMYOSINcoord: 110..138
score: 38.77
coord: 85..105
score: 36.43
coord: 196..221
score: 29.69
coord: 49..66
score: 29.77
coord: 140..163
score: 28.8
IPR000533TropomyosinPFAMPF00261Tropomyosincoord: 14..247
e-value: 1.4E-36
score: 126.0