Glycosyl hydrolase family 25

Overview
NameGlycosyl hydrolase family 25
Smed IDSMED30023196
Length (bp)835
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Glycosyl hydrolase family 25 (SMED30023196) t-SNE clustered cells

Violin plots show distribution of expression levels for Glycosyl hydrolase family 25 (SMED30023196) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Glycosyl hydrolase family 25 (SMED30023196) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Glycosyl hydrolase family 25 (SMED30023196) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30023196

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 10

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
pharynxSMED30023196SMESG000049688.1 SMESG000049683.1 SMESG000049676.1 SMESG000049663.1 SMESG000049649.1 SMESG000049621.1 SMESG000049614.1 SMESG000049603.1 SMESG000049567.1 SMESG000008530.1 SMESG000008521.1 dd_Smed_v4_2003_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30023196SMESG000049688.1 SMESG000049683.1 SMESG000049676.1 SMESG000049663.1 SMESG000049649.1 SMESG000049621.1 SMESG000049614.1 SMESG000049603.1 SMESG000049567.1 SMESG000008530.1 SMESG000008521.1 dd_Smed_v4_2003_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30023196SMESG000049688.1 SMESG000049683.1 SMESG000049676.1 SMESG000049663.1 SMESG000049649.1 SMESG000049621.1 SMESG000049614.1 SMESG000049603.1 SMESG000049567.1 SMESG000008530.1 SMESG000008521.1 dd_Smed_v4_2003_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30023196SMESG000049688.1 SMESG000049683.1 SMESG000049676.1 SMESG000049663.1 SMESG000049649.1 SMESG000049621.1 SMESG000049614.1 SMESG000049603.1 SMESG000049567.1 SMESG000008530.1 SMESG000008521.1 dd_Smed_v4_2003_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30023196SMESG000049688.1 SMESG000049683.1 SMESG000049676.1 SMESG000049663.1 SMESG000049649.1 SMESG000049621.1 SMESG000049614.1 SMESG000049603.1 SMESG000049567.1 SMESG000008530.1 SMESG000008521.1 dd_Smed_v4_2003_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30023196SMESG000049688.1 SMESG000049683.1 SMESG000049676.1 SMESG000049663.1 SMESG000049649.1 SMESG000049621.1 SMESG000049614.1 SMESG000049603.1 SMESG000049567.1 SMESG000008530.1 SMESG000008521.1 dd_Smed_v4_2003_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30023196SMESG000043141.1 Contig3969GPL15192PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30023196SMESG000008530.1 SMESG000008521.1 Contig3969GPL15192PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
epidermisSMED30023196SMESG000049688.1 SMESG000049683.1 SMESG000049676.1 SMESG000049663.1 SMESG000049649.1 SMESG000049621.1 SMESG000049614.1 SMESG000049603.1 SMESG000049567.1 SMESG000008530.1 SMESG000008521.1 dd_Smed_v4_2003_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Category 4 cellSMED30023196SMESG000049688.1 SMESG000049683.1 SMESG000049676.1 SMESG000049663.1 SMESG000049649.1 SMESG000049621.1 SMESG000049614.1 SMESG000049603.1 SMESG000049567.1 SMESG000008530.1 SMESG000008521.1 dd_Smed_v4_2003_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Glycosyl hydrolase family 25 vs. UniProt/SwissProt
Match: sp|P76421|YEGX_ECOLI (Uncharacterized protein YegX OS=Escherichia coli (strain K12) OX=83333 GN=yegX PE=3 SV=2)

HSP 1 Score: 104.76 bits (260), Expect = 2.101e-25
Identity = 71/207 (34.30%), Postives = 108/207 (52.17%), Query Frame = 2 
Query:  158 YNISMIDVSHYNGQINWTMVRKFG-----IMFSCMKATEGTKYVDPMFLTNCNGASKVNINVGAYHFYRPEEDYKLQFKNFLISVRKVNQIKFVA-LDVEQKGKVSKAKYADDIWAFLKLLDNYFRSRVLIYTRKSFWDLNVDWKRHDFTSYLLWVANYRVSKPQIPGNWTNWKIWQFNDRGKIPGINGNVDLNYVNKL*QIRELET 760
            Y I  IDVS +  +I+W  V K       + F+ +KATEG K VDP F  N   + +  +  GAYH++ P     +Q + FL +V   +Q  F A LDVE++GK+S  +    +  +LK+++     + +IY+   F+  N+      F  Y  WVA+Y   +P   G    W+ WQ +DRG++ GING VD N  N    + EL+ 
Sbjct:   65 YTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKATEGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVSASVQARLFLQTV-DFSQGDFPAVLDVEERGKLSAKELRKRVSQWLKMVEKSTGKKPIIYSGAVFYHTNLAGY---FNEYPWWVAHYYQRRPDNDG--MAWRFWQHSDRGQVDGINGPVDFNVFNGT--VEELQA 263
[Close]
BLAST of Glycosyl hydrolase family 25 vs. UniProt/SwissProt
Match: sp|Q8FFY2|YEGX_ECOL6 (Uncharacterized protein YegX OS=Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC) OX=199310 GN=yegX PE=3 SV=2)

HSP 1 Score: 104.76 bits (260), Expect = 2.236e-25
Identity = 69/197 (35.03%), Postives = 104/197 (52.79%), Query Frame = 2 
Query:  158 YNISMIDVSHYNGQINWTMVRKFG-----IMFSCMKATEGTKYVDPMFLTNCNGASKVNINVGAYHFYRPEEDYKLQFKNFLISVRKVNQIKFVA-LDVEQKGKVSKAKYADDIWAFLKLLDNYFRSRVLIYTRKSFWDLNVDWKRHDFTSYLLWVANYRVSKPQIPGNWTNWKIWQFNDRGKIPGINGNVDLNYVN 730
            Y I  IDVS +  +I+W  V K       + F+ +KATEG K VDP F  N   + +  +  GAYH++ P     +Q + FL +V   +Q  F A LDVE++GK+S  +    +  +LK+++     + +IY+   F+  N+      F  Y  WVA+Y   +P   G    W+ WQ +DRG++ GING VD N  N
Sbjct:   65 YTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKATEGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVSASVQARLFLQTV-DFSQGDFPAVLDVEERGKLSAKELRKRVSQWLKMVEKRTGKKPIIYSGAVFYHTNLAGY---FNEYPWWVAHYYQRRPDNDG--MAWRFWQHSDRGQVDGINGPVDFNVFN 255
[Close]
BLAST of Glycosyl hydrolase family 25 vs. UniProt/SwissProt
Match: sp|Q8X7H0|YEGX_ECO57 (Uncharacterized protein YegX OS=Escherichia coli O157:H7 OX=83334 GN=yegX PE=3 SV=2)

HSP 1 Score: 104.76 bits (260), Expect = 2.405e-25
Identity = 69/197 (35.03%), Postives = 105/197 (53.30%), Query Frame = 2 
Query:  158 YNISMIDVSHYNGQINWTMVRKFG-----IMFSCMKATEGTKYVDPMFLTNCNGASKVNINVGAYHFYRPEEDYKLQFKNFLISVRKVNQIKFVA-LDVEQKGKVSKAKYADDIWAFLKLLDNYFRSRVLIYTRKSFWDLNVDWKRHDFTSYLLWVANYRVSKPQIPGNWTNWKIWQFNDRGKIPGINGNVDLNYVN 730
            Y I  IDVS +  +I+W  V K       + F+ +KATEG K VDP F  N   + +  +  GAYH++ P     +Q + FL +V   +Q  F A LDVE++GK+S  +    +  +LK+++     + +IY+   F+  N+      F  Y  WVA+Y   +P   G    W+ WQ +DRG++ GING+VD N  N
Sbjct:   65 YTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKATEGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVAAPVQARLFLQTV-DFSQGDFPAVLDVEERGKLSAKELRKRVSQWLKMVEKSTGKKPVIYSGAVFYHTNLAGY---FNEYPWWVAHYYQRRPDNDG--MAWRFWQHSDRGQVDGINGSVDFNVFN 255
[Close]
BLAST of Glycosyl hydrolase family 25 vs. UniProt/SwissProt
Match: sp|P25310|LYSM1_STRGL (Lysozyme M1 OS=Streptomyces globisporus OX=1908 GN=acm PE=1 SV=1)

HSP 1 Score: 100.523 bits (249), Expect = 1.310e-23
Identity = 61/200 (30.50%), Postives = 94/200 (47.00%), Query Frame = 2 
Query:  161 NISMIDVSHYNGQINWTMVRKFGIMFSCMKATEGTKYVDPMFLTNCNGASKVNINVGAYHFYRPEEDYKLQFKNFLISV----RKVNQIKFVALDVEQ--KGKVSKAKYADDIWAFLKLLDNYFRSR----VLIYTRKSFWDLNVDWKRHDFTSYLLWVANYRVSKPQIPGNWTNWKIWQFNDRGKIPGINGNVDLNYVN 730
             +  IDVSH+ G INW+ V+  G+ F+ +KATEGT Y D  F  N   A    I  GAYHF RP         ++  S      + N+     LD+E    G +        +  ++      +++R    V+IYT  S+W+               WVA++ VS P +P  +  W  WQ++  G++ G++G+VD N  N
Sbjct:   81 GVQGIDVSHWQGSINWSSVKSAGMSFAYIKATEGTNYKDDRFSANYTNAYNAGIIRGAYHFARPNASSGTAQADYFASNGGGWSRDNRTLPGVLDIEHNPSGAMCYGLSTTQMRTWINDFHARYKARTTRDVVIYTTASWWNTCTGSWNGMAAKSPFWVAHWGVSAPTVPSGFPTWTFWQYSATGRVGGVSGDVDRNKFN 280
[Close]
BLAST of Glycosyl hydrolase family 25 vs. UniProt/SwissProt
Match: sp|P26836|LYS_CLOPE (Probable autolytic lysozyme OS=Clostridium perfringens (strain 13 / Type A) OX=195102 GN=lyc PE=3 SV=2)

HSP 1 Score: 98.2117 bits (243), Expect = 1.663e-22
Identity = 61/190 (32.11%), Postives = 99/190 (52.11%), Query Frame = 2 
Query:  161 NISMIDVSHYNGQINWTMVRKFGIMFSCMKATEGTKYVDPMFLTNCNGASKVNINVGAYHFYRPEEDYKLQFKNFLISVRKVNQIKF---VALDVEQKGKVSKAKYADDIWAFLKLLDNYFRSRVLIYTRKSFWDLNVDWKRHDFTSYLLWVANYRVSKPQIPGNWTNWKIWQFNDRGKIPGINGNVDLN 721
            N+  IDVS++ G IN+  V+  GI    +KATEG  + D     N   A    + VG YHF++P ++ K Q K F+  + ++    +   +ALDVE     S  +       FL+ +       V++YT  SF + N+D   +    Y LW+A Y V  P+    W++W  +Q++D+G + G++GN D+N
Sbjct:    8 NLKGIDVSNWQGNINFKSVKNEGIEVVYIKATEGDYFKDSYAKQNYKRAKAEGLKVGFYHFFKPNKNAKRQAKYFIDYLNEIGATDYDCKLALDVETTEGRSAYELTTMCIEFLEEVRKITNREVVVYTYTSFANNNLD---NRLGVYPLWIAEYGVKAPKDNRVWSSWIGFQYSDKGNVAGVSGNCDMN 194
[Close]
BLAST of Glycosyl hydrolase family 25 vs. TrEMBL
Match: A0A3N2QBE5 (Glycosyl hydrolase family 25 OS=Candidatus Cardinium hertigii OX=247481 GN=EDM02_05275 PE=4 SV=1)

HSP 1 Score: 143.665 bits (361), Expect = 3.571e-38
Identity = 75/192 (39.06%), Postives = 115/192 (59.90%), Query Frame = 2 
Query:  173 IDVSHYNGQINWTMVRKFGIMFSCMKATEGTKYVDPMFLTNCNGASKVNINVGAYHFYRPEEDYKLQFKNFLISVRKVN---QIKFVALDVEQK--GKVSKAKYADDIWAFLKLLDNYFRSRVLIYTRKSFWDLNVDWKRHDFTSYLLWVANYRVSKPQIPGNWTN--WKIWQFNDRGKIPGINGNVDLNYV 727
            IDVS Y G INW  V+K  I ++ ++AT G    D  F+ N  GA  V I  GAYH++R     + Q +N +  + K++       + +DVE+K   + +  + AD  +  LK+L N     + IY   ++W  +V+W+++DF+ YLLW+A++RV  P +P  W N  WKIWQF+++G+IPGI GNVDL+ V
Sbjct:   23 IDVSKYQGDINWVEVQKANIEYAMIRATSGI-VKDERFVANWEGAKAVGIRPGAYHYFRANYTAQEQAENLIERLSKISFNPYTDVLVIDVEKKFNEEATPDQMADGTYELLKILQNTSYEHLGIYASPNYWTNDVNWRKYDFSQYLLWIAHWRVQAPLVPETWINVGWKIWQFSNKGQIPGITGNVDLDIV 213
[Close]
BLAST of Glycosyl hydrolase family 25 vs. TrEMBL
Match: A0A1M6ZH87 (Glycosyl hydrolases family 25 OS=Labrenzia suaedae OX=735517 GN=SAMN05444272_0239 PE=4 SV=1)

HSP 1 Score: 141.739 bits (356), Expect = 1.189e-37
Identity = 73/198 (36.87%), Postives = 107/198 (54.04%), Query Frame = 2 
Query:  143 MASVLYNISMIDVSHYNGQINWTMVRKFGIMFSCMKATEGTKYVDPMFLTNCNGASKVNINVGAYHFYRPEEDYKLQFKNFLISVRKVNQIKFV----ALDVEQKGKVSKAKYADDIWAFLKLLDNYFRSRVLIYTRKSFWDLNVDWKRHDFTSYLLWVANY-RVSKPQIPGNWTNWKIWQFNDRGKIPGINGNVDLN 721
            M++ + N++ IDVSHY G INW  V      F+ +KAT+GT +VDPMF TN  GA+   +  GAYH++ PE+ +  Q    L  ++ V          A+D E    VS A Y   + + + +L        +IYT  +FWD   D    DF+   LW+ANY    +P +PG+W+ + IWQ+   G + G+ GNVD N
Sbjct:    1 MSTTVQNLTGIDVSHYQGDINWQEVAANPTSFAIIKATQGTGFVDPMFQTNWQGANAAGLRTGAYHYFMPEKSFLEQADLLLTQLKAVGYDASADLPPAIDCEVMDGVSNAAYTFALSSLVDILRRQLGVSPMIYTAPAFWDALGD---PDFSECPLWIANYTSADQPTLPGSWSGYAIWQYTSEGSVNGVEGNVDRN 195
[Close]
BLAST of Glycosyl hydrolase family 25 vs. TrEMBL
Match: A0A562T0S0 (Lysozyme OS=Roseibium hamelinense OX=150831 GN=JM93_02406 PE=3 SV=1)

HSP 1 Score: 139.043 bits (349), Expect = 1.382e-36
Identity = 70/205 (34.15%), Postives = 106/205 (51.71%), Query Frame = 2 
Query:  173 IDVSHYNGQINWTMVRKFGIMFSCMKATEGTKYVDPMFLTNCNGASKVNINVGAYHFYRPEEDYKLQFKNFLISVRKVNQIKFV----ALDVEQKGKVSKAKYADDIWAFLKLLDNYFRSRVLIYTRKSFWDLNVDWKRHDFTSYLLWVANYR-VSKPQIPGNWTNWKIWQFNDRGKIPGINGNVDLNYVNKL*QIRELETV*LK 772
            IDVSHY G +NW  V   GI F+ +KATEGT Y DP F  N +G  +  +  G YH++ P + +  Q +  + ++R V   +      A+D E+    S A+Y   +   + L++ +   R +IYT  SFWD        DF+ Y LW+A Y     P +P  W+++ IWQ++D+G + GI G  D +  N    +    T  LK
Sbjct:    9 IDVSHYQGTVNWQGVAASGIDFAYLKATEGTGYTDPTFKQNWSGCQEYALKCGGYHYFLPRDSFLQQAEAIVQAMRSVGYSRTTDLPPAIDCEEMDGCSAAEYVHALAGLVSLVERFLCVRPMIYTNPSFWD---GLGNPDFSEYPLWLAQYTDAQSPTVPAPWSSYTIWQYSDQGTVSGIGGFCDCDLANSSLNVSPPGTSILK 210
[Close]
BLAST of Glycosyl hydrolase family 25 vs. TrEMBL
Match: A0A2D7NJF0 (Uncharacterized protein OS=Planctomycetaceae bacterium OX=2026779 GN=CMJ57_06930 PE=4 SV=1)

HSP 1 Score: 135.961 bits (341), Expect = 4.635e-35
Identity = 73/215 (33.95%), Postives = 109/215 (50.70%), Query Frame = 2 
Query:  107 FAMNLVGIWTMQMASVLYN-----ISMIDVSHYNGQINWTMVRKFGIMFSCMKATEGTKYVDPMFLTNCNGASKVNINVGAYHFYRPEEDYKLQFKNFLISVRKVNQIKFVALDVEQKGKVSKAKYADDIWAFLKLLDNYFRSRVLIYTRKSFWDLNVDWKRHDFTSYLLWVANYRVSKPQIPGNWTNWKIWQFNDRGKIPGINGNVDLNYVNKL 736
             ++ L G    Q A          +  IDVSH+ G I+W  V   GI F+ +KAT+GTK+VDP F  N +G +K  I  GAYHF  P+ D   Q K+FL +V          +DVE KG   +     ++  FL  +    +   +IY   +FW+ +++  R D     LW+A Y VS P+  G+   W +WQ+   G++PGI G VD +   ++
Sbjct:   14 LSLALTGAAAAQEAPETTPQEDEVVEGIDVSHFQGAIDWPTVEGAGIRFAYIKATQGTKWVDPRFTENWSGVAKTGIRRGAYHFLEPDVDGTAQAKHFLKTVELKEGDLLPVVDVETKGPNLRV----NLEKFLAEIKRQTKLDAMIYVAPAFWNDHLETSREDPWPNPLWIAEYGVSTPRAAGSLGPWLVWQYEQDGRVPGITGPVDRDRARRV 224
[Close]
BLAST of Glycosyl hydrolase family 25 vs. TrEMBL
Match: A0A5J6PB45 (Glycosyl hydrolase family 25 OS=Cellvibrio sp. KY-GH-1 OX=2303332 GN=D0C16_17420 PE=4 SV=1)

HSP 1 Score: 132.88 bits (333), Expect = 1.825e-34
Identity = 70/192 (36.46%), Postives = 102/192 (53.12%), Query Frame = 2 
Query:  164 ISMIDVSHYNGQINWTMVRKFGIMFSCMKATEGTKYVDPMFLTNCNGASKVNINVGAYHFYRPEEDYKLQFKNFLISVRKVNQIKFVA-LDVEQ-KGKVSKAKYADDIWAFLKLLDNYFRSRVLIYTRKSFWDLNVDWKRHDFTSYLLWVANYRVSKPQIPGNWTNWKIWQFNDRGKIPGINGNVDLNYVNK 733
            +  IDVSHY G ++W  V+  GI F+  KAT+G  Y DP F TN        +  GAYHFY   +D   Q  NF+ +V  ++       +D+E  KG       A ++  +L  ++       +IYT  SFW+  ++    DF+ Y LWVA Y V+ P+IP  WT W  WQ++  G + G+ G+VDL+  NK
Sbjct:    7 LQGIDVSHYQGTVDWKKVKAAGIAFAYAKATDGNTYTDPQFHTNWQAMQAAGVLRGAYHFYETNDDPVTQANNFVAAVGSLDANDLAPVVDIEIFKGNYGSNSVAANLQIWLDTVEKALGRTPMIYTGPSFWNQTIN---SDFSKYPLWVAEYGVTTPKIPFGWTAWNFWQYSQSGNVDGVTGSVDLDVWNK 195
[Close]
BLAST of Glycosyl hydrolase family 25 vs. Planmine SMEST
Match: SMESG000008530.1 (SMESG000008530.1)

HSP 1 Score: 404.831 bits (1039), Expect = 6.522e-145
Identity = 196/198 (98.99%), Postives = 196/198 (98.99%), Query Frame = 2 
Query:  143 MASVLYNISMIDVSHYNGQINWTMVRKFGIMFSCMKATEGTKYVDPMFLTNCNGASKVNINVGAYHFYRPEEDYKLQFKNFLISVRKVNQIKFVALDVEQKGKVSKAKYADDIWAFLKLLDNYFRSRVLIYTRKSFWDLNVDWKRHDFTSYLLWVANYRVSKPQIPGNWTNWKIWQFNDRGKIPGINGNVDLNYVNKL 736
            MASVLYNISMIDVSHYNGQINWTMVRKFGIMFSCMKATEGTKYVDPMFLTNCNGASKVNINVGAYHFYRPEEDYKLQF NFLISVRKVNQIKFVALDVEQKGKVSKAKYADDIWAFLKLLD YFRSRVLIYTRKSFWDLNVDWKRHDFTSYLLWVANYRVSKPQIPGNWTNWKIWQFNDRGKIPGINGNVDLNYVNKL
Sbjct:    1 MASVLYNISMIDVSHYNGQINWTMVRKFGIMFSCMKATEGTKYVDPMFLTNCNGASKVNINVGAYHFYRPEEDYKLQFNNFLISVRKVNQIKFVALDVEQKGKVSKAKYADDIWAFLKLLDKYFRSRVLIYTRKSFWDLNVDWKRHDFTSYLLWVANYRVSKPQIPGNWTNWKIWQFNDRGKIPGINGNVDLNYVNKL 198
[Close]
BLAST of Glycosyl hydrolase family 25 vs. Planmine SMEST
Match: SMESG000008521.1 (SMESG000008521.1)

HSP 1 Score: 400.979 bits (1029), Expect = 2.538e-143
Identity = 194/198 (97.98%), Postives = 195/198 (98.48%), Query Frame = 2 
Query:  143 MASVLYNISMIDVSHYNGQINWTMVRKFGIMFSCMKATEGTKYVDPMFLTNCNGASKVNINVGAYHFYRPEEDYKLQFKNFLISVRKVNQIKFVALDVEQKGKVSKAKYADDIWAFLKLLDNYFRSRVLIYTRKSFWDLNVDWKRHDFTSYLLWVANYRVSKPQIPGNWTNWKIWQFNDRGKIPGINGNVDLNYVNKL 736
            MASVLYNISMIDVSHYNGQINWTMVRKFGIMFSCMKATEGTKYVDPMFLTNCNGASKVNINVGAYHFYRPEEDY+LQFK FLISV KVNQIKFVALDVEQKGKVSKAKYADDIWAFLKLLD YFRSRVLIYTRKSFWDLNVDWKRHDFTSYLLWVANYRVSKPQIPGNWTNWKIWQFNDRGKIPGINGNVDLNYVNKL
Sbjct:    1 MASVLYNISMIDVSHYNGQINWTMVRKFGIMFSCMKATEGTKYVDPMFLTNCNGASKVNINVGAYHFYRPEEDYELQFKKFLISVGKVNQIKFVALDVEQKGKVSKAKYADDIWAFLKLLDKYFRSRVLIYTRKSFWDLNVDWKRHDFTSYLLWVANYRVSKPQIPGNWTNWKIWQFNDRGKIPGINGNVDLNYVNKL 198
The following BLAST results are available for this feature:
BLAST of Glycosyl hydrolase family 25 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Glycosyl hydrolase family 25 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Glycosyl hydrolase family 25 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Glycosyl hydrolase family 25 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Glycosyl hydrolase family 25 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Glycosyl hydrolase family 25 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Glycosyl hydrolase family 25 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|P76421|YEGX_ECOLI2.101e-2534.30Uncharacterized protein YegX OS=Escherichia coli (... [more]
sp|Q8FFY2|YEGX_ECOL62.236e-2535.03Uncharacterized protein YegX OS=Escherichia coli O... [more]
sp|Q8X7H0|YEGX_ECO572.405e-2535.03Uncharacterized protein YegX OS=Escherichia coli O... [more]
sp|P25310|LYSM1_STRGL1.310e-2330.50Lysozyme M1 OS=Streptomyces globisporus OX=1908 GN... [more]
sp|P26836|LYS_CLOPE1.663e-2232.11Probable autolytic lysozyme OS=Clostridium perfrin... [more]
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BLAST of Glycosyl hydrolase family 25 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A3N2QBE53.571e-3839.06Glycosyl hydrolase family 25 OS=Candidatus Cardini... [more]
A0A1M6ZH871.189e-3736.87Glycosyl hydrolases family 25 OS=Labrenzia suaedae... [more]
A0A562T0S01.382e-3634.15Lysozyme OS=Roseibium hamelinense OX=150831 GN=JM9... [more]
A0A2D7NJF04.635e-3533.95Uncharacterized protein OS=Planctomycetaceae bacte... [more]
A0A5J6PB451.825e-3436.46Glycosyl hydrolase family 25 OS=Cellvibrio sp. KY-... [more]
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BLAST of Glycosyl hydrolase family 25 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Glycosyl hydrolase family 25 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Glycosyl hydrolase family 25 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Glycosyl hydrolase family 25 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Glycosyl hydrolase family 25 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Glycosyl hydrolase family 25 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000008530.16.522e-14598.99SMESG000008530.1[more]
SMESG000008521.12.538e-14397.98SMESG000008521.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30023196 ID=SMED30023196|Name=Glycosyl hydrolase family 25|organism=Schmidtea mediterranea sexual|type=transcript|length=835bp
TCCAATGATAGGTCAATAGTAAACAATGCACAATTTTTATTGATTTTTTT
GTAAAAATAGATTTATTAATTCATTTGTTAAATCTTAAAAACGAATTTTT
TAGAAGTTTGCAATGAATTTGGTGGGTATTTGGACAATGCAAATGGCAAG
CGTTTTATACAATATTAGCATGATTGATGTTTCCCATTACAATGGACAAA
TAAACTGGACAATGGTGAGGAAGTTTGGTATTATGTTCTCATGTATGAAA
GCGACCGAGGGAACAAAATATGTAGATCCGATGTTTCTTACTAATTGCAA
TGGAGCGTCTAAAGTCAATATCAATGTCGGTGCTTATCACTTTTACAGAC
CAGAGGAAGACTATAAGTTGCAATTTAAAAATTTTTTAATATCCGTACGT
AAAGTGAATCAAATAAAGTTTGTAGCCCTGGATGTGGAACAAAAAGGAAA
AGTTTCAAAAGCCAAATATGCCGATGACATTTGGGCGTTTCTAAAACTCC
TTGATAATTATTTCCGGTCGAGGGTATTAATTTATACTAGAAAATCGTTT
TGGGATCTTAATGTTGATTGGAAGCGACATGATTTCACGTCATATTTGCT
CTGGGTAGCGAATTATCGCGTTTCTAAGCCACAAATACCTGGAAATTGGA
CCAATTGGAAAATATGGCAATTCAATGATCGCGGAAAAATACCGGGAATT
AATGGAAATGTTGATCTAAATTATGTAAATAAATTATAGCAAATTCGTGA
ATTAGAAACTGTATAACTCAAATAGGATAGTTATTAATAATTTAAATTTA
ATATTAGTTTTATTGTGATGTAATTAATAATAAAT
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protein sequence of SMED30023196-orf-1

>SMED30023196-orf-1 ID=SMED30023196-orf-1|Name=SMED30023196-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=209bp
MNLVGIWTMQMASVLYNISMIDVSHYNGQINWTMVRKFGIMFSCMKATEG
TKYVDPMFLTNCNGASKVNINVGAYHFYRPEEDYKLQFKNFLISVRKVNQ
IKFVALDVEQKGKVSKAKYADDIWAFLKLLDNYFRSRVLIYTRKSFWDLN
VDWKRHDFTSYLLWVANYRVSKPQIPGNWTNWKIWQFNDRGKIPGINGNV
DLNYVNKL*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000018Category 4 cell
PLANA:0000034epidermis
PLANA:0002032epidermal cell
PLANA:0003116parenchymal cell
Vocabulary: INTERPRO
TermDefinition
IPR018077Glyco_hydro_fam25_subgr
IPR013781Glyco_hydro_catalytic_dom
IPR002053Glyco_hydro_25
IPR017853Glycoside_hydrolase_SF
Vocabulary: biological process
TermDefinition
GO:0005975carbohydrate metabolic process
GO:0009253peptidoglycan catabolic process
GO:0016998cell wall macromolecule catabolic process
Vocabulary: molecular function
TermDefinition
GO:0003796lysozyme activity
GO:0016787hydrolase activity
Vocabulary: cellular component
TermDefinition
GO:0016020membrane
GO:0016021integral component of membrane
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR018077Glycoside hydrolase, family 25 subgroupSMARTSM00641glyco_25coord: 18..123
e-value: 3.3E-8
score: 43.3
NoneNo IPR availableGENE3DG3DSA:3.20.20.80coord: 7..208
e-value: 9.5E-58
score: 197.3
NoneNo IPR availablePANTHERPTHR34135:SF2coord: 11..205
NoneNo IPR availablePANTHERPTHR34135FAMILY NOT NAMEDcoord: 11..205
NoneNo IPR availablePROSITEPS51904GLYCOSYL_HYDROL_F25_2coord: 17..208
score: 46.762
NoneNo IPR availableCDDcd00599GH25_muramidasecoord: 20..205
e-value: 6.10824E-54
score: 168.681
IPR002053Glycoside hydrolase, family 25PFAMPF01183Glyco_hydro_25coord: 21..196
e-value: 6.0E-42
score: 143.8
IPR017853Glycoside hydrolase superfamilySUPERFAMILYSSF51445(Trans)glycosidasescoord: 18..206