60s ribosomal protein L14
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30023172 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 14
Homology
BLAST of 60s ribosomal protein L14 vs. Ensembl Human
Match: RPL14 (ribosomal protein L14 [Source:HGNC Symbol;Acc:HGNC:10305]) HSP 1 Score: 109.768 bits (273), Expect = 3.504e-29 Identity = 48/121 (39.67%), Postives = 79/121 (65.29%), Query Frame = 3 Query: 108 VQCGRVAFIATGEDQGKLCAIVEIIDQNRVLIDGPLSGVVRKSVNISNLHLTSFNIHLPHGSRTKVVRKEWLKAEISKKWEESTWCQKLKKKAIRAGLNDYERFQVRVAIRNKTRLIAQEM 470 V+ GRVA+++ G GKL AIV++IDQNR L+DGP + V R+++ + LT F + PH + K VR+ W KA+I+ KW + W +K++ + +A + D++RF+V A + + R+I E+ Sbjct: 7 VEVGRVAYVSFGPHAGKLVAIVDVIDQNRALVDGPCTQVRRQAMPFKCMQLTDFILKFPHSAHQKYVRQAWQKADINTKWAATRWAKKIEARERKAKMTDFDRFKVMKAKKMRNRIIKNEV 127
BLAST of 60s ribosomal protein L14 vs. Ensembl Human
Match: RPL14 (ribosomal protein L14 [Source:HGNC Symbol;Acc:HGNC:10305]) HSP 1 Score: 109.768 bits (273), Expect = 3.504e-29 Identity = 48/121 (39.67%), Postives = 79/121 (65.29%), Query Frame = 3 Query: 108 VQCGRVAFIATGEDQGKLCAIVEIIDQNRVLIDGPLSGVVRKSVNISNLHLTSFNIHLPHGSRTKVVRKEWLKAEISKKWEESTWCQKLKKKAIRAGLNDYERFQVRVAIRNKTRLIAQEM 470 V+ GRVA+++ G GKL AIV++IDQNR L+DGP + V R+++ + LT F + PH + K VR+ W KA+I+ KW + W +K++ + +A + D++RF+V A + + R+I E+ Sbjct: 7 VEVGRVAYVSFGPHAGKLVAIVDVIDQNRALVDGPCTQVRRQAMPFKCMQLTDFILKFPHSAHQKYVRQAWQKADINTKWAATRWAKKIEARERKAKMTDFDRFKVMKAKKMRNRIIKNEV 127
BLAST of 60s ribosomal protein L14 vs. Ensembl Human
Match: RPL14 (ribosomal protein L14 [Source:HGNC Symbol;Acc:HGNC:10305]) HSP 1 Score: 104.375 bits (259), Expect = 2.851e-28 Identity = 44/106 (41.51%), Postives = 71/106 (66.98%), Query Frame = 3 Query: 108 VQCGRVAFIATGEDQGKLCAIVEIIDQNRVLIDGPLSGVVRKSVNISNLHLTSFNIHLPHGSRTKVVRKEWLKAEISKKWEESTWCQKLKKKAIRAGLNDYERFQV 425 V+ GRVA+++ G GKL AIV++IDQNR L+DGP + V R+++ + LT F + PH + K VR+ W KA+I+ KW + W +K++ + +A + D++RF+V Sbjct: 7 VEVGRVAYVSFGPHAGKLVAIVDVIDQNRALVDGPCTQVRRQAMPFKCMQLTDFILKFPHSAHQKYVRQAWQKADINTKWAATRWAKKIEARERKAKMTDFDRFKV 112
BLAST of 60s ribosomal protein L14 vs. Ensembl Celegans
Match: rpl-14 (Ribosomal Protein, Large subunit [Source:UniProtKB/TrEMBL;Acc:Q9XVE9]) HSP 1 Score: 112.849 bits (281), Expect = 8.368e-32 Identity = 54/123 (43.90%), Postives = 84/123 (68.29%), Query Frame = 3 Query: 102 KTVQCGRVAFIATGEDQGKLCAIVEIIDQNRVLIDGPLSGVVRKSVNISNLHLTSFNIHLPHGSRTKVVRKEWLKAEISKKWEESTWCQKLKKKAIRAGLNDYERFQVRVAIRNKTRLIAQEM 470 + VQ GRV FIA+G+DQGKL AIV +ID NRV IDGP S V R N+ +L LT F + L G RTK V+ + A++++ ++++ W +K+ ++AIRA L D+ER+++ A + + R++ E+ Sbjct: 5 RVVQIGRVVFIASGKDQGKLAAIVNVIDGNRVQIDGPSSDVTRTVRNLKDLQLTKFVLKLRVGQRTKGVKAAFDAAKVTENFQKTQWAKKIAQRAIRAKLTDFERYKLMKAKQMRNRIVRVEL 127
BLAST of 60s ribosomal protein L14 vs. Ensembl Celegans
Match: rpl-14 (Ribosomal Protein, Large subunit [Source:UniProtKB/TrEMBL;Acc:Q9XVE9]) HSP 1 Score: 112.849 bits (281), Expect = 8.368e-32 Identity = 54/123 (43.90%), Postives = 84/123 (68.29%), Query Frame = 3 Query: 102 KTVQCGRVAFIATGEDQGKLCAIVEIIDQNRVLIDGPLSGVVRKSVNISNLHLTSFNIHLPHGSRTKVVRKEWLKAEISKKWEESTWCQKLKKKAIRAGLNDYERFQVRVAIRNKTRLIAQEM 470 + VQ GRV FIA+G+DQGKL AIV +ID NRV IDGP S V R N+ +L LT F + L G RTK V+ + A++++ ++++ W +K+ ++AIRA L D+ER+++ A + + R++ E+ Sbjct: 5 RVVQIGRVVFIASGKDQGKLAAIVNVIDGNRVQIDGPSSDVTRTVRNLKDLQLTKFVLKLRVGQRTKGVKAAFDAAKVTENFQKTQWAKKIAQRAIRAKLTDFERYKLMKAKQMRNRIVRVEL 127
BLAST of 60s ribosomal protein L14 vs. Ensembl Fly
Match: RpL14 (gene:FBgn0017579 transcript:FBtr0076633) HSP 1 Score: 119.398 bits (298), Expect = 7.725e-34 Identity = 51/117 (43.59%), Postives = 82/117 (70.09%), Query Frame = 3 Query: 108 VQCGRVAFIATGEDQGKLCAIVEIIDQNRVLIDGPLSGVVRKSVNISNLHLTSFNIHLPHGSRTKVVRKEWLKAEISKKWEESTWCQKLKKKAIRAGLNDYERFQVRVAIRNKTRLI 458 VQ GR+A + G +G+L AIV++IDQNRVL+DGPL+GV R+ ++NLHLT + I P+ + T++VRK W ++++ +W+ S W K + R+ LND++RF++R A R + +L+ Sbjct: 7 VQTGRIAKASAGPLKGRLVAIVDVIDQNRVLVDGPLTGVPRQEYRLNNLHLTKYRIKFPYTAPTRIVRKAWTESDLKAQWKVSPWSVKAQNICKRSSLNDFDRFKLRYAKRQRNKLL 123
BLAST of 60s ribosomal protein L14 vs. Ensembl Zebrafish
Match: rpl14 (ribosomal protein L14 [Source:ZFIN;Acc:ZDB-GENE-030131-2085]) HSP 1 Score: 133.265 bits (334), Expect = 1.547e-39 Identity = 60/121 (49.59%), Postives = 88/121 (72.73%), Query Frame = 3 Query: 108 VQCGRVAFIATGEDQGKLCAIVEIIDQNRVLIDGPLSGVVRKSVNISNLHLTSFNIHLPHGSRTKVVRKEWLKAEISKKWEESTWCQKLKKKAIRAGLNDYERFQVRVAIRNKTRLIAQEM 470 V+ GRVAFIA G +GKL AIV++IDQNR L+DGP +GV R+++ L LT + I +PH +R K VR+ W KAEISKKWEES+W +K++ + RA ++D++R++V A + + ++I EM Sbjct: 7 VEIGRVAFIAFGPHEGKLVAIVDVIDQNRALVDGPCTGVKRQALPFKCLQLTDYVIKVPHSARQKYVRRAWEKAEISKKWEESSWAKKIEARKKRAAMSDFDRYKVSKAKKMRNKIIRHEM 127
BLAST of 60s ribosomal protein L14 vs. Ensembl Xenopus
Match: myl10 (myosin light chain 10 [Source:Xenbase;Acc:XB-GENE-986520]) HSP 1 Score: 116.701 bits (291), Expect = 7.217e-33 Identity = 51/123 (41.46%), Postives = 82/123 (66.67%), Query Frame = 3 Query: 102 KTVQCGRVAFIATGEDQGKLCAIVEIIDQNRVLIDGPLSGVVRKSVNISNLHLTSFNIHLPHGSRTKVVRKEWLKAEISKKWEESTWCQKLKKKAIRAGLNDYERFQVRVAIRNKTRLIAQEM 470 + VQ GRVA+++ G GKL AIV++IDQNR L+DGP +GV R+++ + LT F I +PH +R K VR+ W KA +++KW S W +K+ + +A + D++R++V A + + ++I EM Sbjct: 5 RYVQIGRVAYVSFGPHAGKLVAIVDVIDQNRALVDGPCTGVRRQAMPFKCMQLTDFVIKVPHSARQKYVRRAWEKANVNEKWTASNWAKKIDARQRKAKMTDFDRYKVMKAKKMRNKIIRHEM 127
BLAST of 60s ribosomal protein L14 vs. Ensembl Xenopus
Match: capn7 (calpain 7 [Source:Xenbase;Acc:XB-GENE-996630]) HSP 1 Score: 110.538 bits (275), Expect = 1.874e-30 Identity = 48/123 (39.02%), Postives = 81/123 (65.85%), Query Frame = 3 Query: 102 KTVQCGRVAFIATGEDQGKLCAIVEIIDQNRVLIDGPLSGVVRKSVNISNLHLTSFNIHLPHGSRTKVVRKEWLKAEISKKWEESTWCQKLKKKAIRAGLNDYERFQVRVAIRNKTRLIAQEM 470 + +Q GRVA+++ G GKL AIV++IDQNR L+DGP +GV R+++ + LT F I +PH +R K VR+ W KA +++KW S W +K+ + +A + D++ ++V A + + ++I E+ Sbjct: 5 RYIQIGRVAYVSFGPHAGKLVAIVDVIDQNRALVDGPCTGVRRQAMPFKCMQLTDFVIKVPHSARQKYVRRAWEKANVNEKWSASNWAKKIDARQRKAKMTDFDCYKVMKAKKMRNKIIRHEI 127
BLAST of 60s ribosomal protein L14 vs. Ensembl Mouse
Match: Rpl14 (ribosomal protein L14 [Source:MGI Symbol;Acc:MGI:1914365]) HSP 1 Score: 112.464 bits (280), Expect = 1.968e-30 Identity = 51/121 (42.15%), Postives = 79/121 (65.29%), Query Frame = 3 Query: 108 VQCGRVAFIATGEDQGKLCAIVEIIDQNRVLIDGPLSGVVRKSVNISNLHLTSFNIHLPHGSRTKVVRKEWLKAEISKKWEESTWCQKLKKKAIRAGLNDYERFQVRVAIRNKTRLIAQEM 470 V+ GRVA+I+ G GKL AIV++IDQNR L+DGP + V R+++ + LT F + PH +R K VRK W KA+I+ KW + W +K+ + +A + D++RF+V A + + R+I E+ Sbjct: 7 VEVGRVAYISFGPHAGKLVAIVDVIDQNRALVDGPCTRVRRQAMPFKCMQLTDFILKFPHSARQKYVRKAWEKADINTKWAATRWAKKIDARERKAKMTDFDRFKVMKAKKMRNRIIKTEV 127
BLAST of 60s ribosomal protein L14 vs. UniProt/SwissProt
Match: sp|O46160|RL14_LUMRU (60S ribosomal protein L14 OS=Lumbricus rubellus OX=35632 GN=RPL14 PE=3 SV=1) HSP 1 Score: 122.479 bits (306), Expect = 3.343e-34 Identity = 61/121 (50.41%), Postives = 78/121 (64.46%), Query Frame = 3 Query: 108 VQCGRVAFIATGEDQGKLCAIVEIIDQNRVLIDGPLSGVVRKSVNISNLHLTSFNIHLPHGSRTKVVRKEWLKAEISKKWEESTWCQKLKKKAIRAGLNDYERFQVRVAIRNKTRLIAQEM 470 V+ GRVA G DQGKL AIV++IDQNR L+DGP + V RKS+N L LT+ PH ++T VV+K W K EISKKWEES +K+ K R L D+ERF++ A + + RLI E Sbjct: 7 VEIGRVARAVYGPDQGKLVAIVDVIDQNRALVDGPCTHVARKSMNFKELELTNLKAKFPHSAKTGVVKKAWEKDEISKKWEESHLAKKIAAKEKRKTLTDFERFKLMKAKQARNRLIKIEF 127
BLAST of 60s ribosomal protein L14 vs. UniProt/SwissProt
Match: sp|P55841|RL14_DROME (60S ribosomal protein L14 OS=Drosophila melanogaster OX=7227 GN=RpL14 PE=1 SV=1) HSP 1 Score: 119.398 bits (298), Expect = 7.744e-33 Identity = 51/117 (43.59%), Postives = 82/117 (70.09%), Query Frame = 3 Query: 108 VQCGRVAFIATGEDQGKLCAIVEIIDQNRVLIDGPLSGVVRKSVNISNLHLTSFNIHLPHGSRTKVVRKEWLKAEISKKWEESTWCQKLKKKAIRAGLNDYERFQVRVAIRNKTRLI 458 VQ GR+A + G +G+L AIV++IDQNRVL+DGPL+GV R+ ++NLHLT + I P+ + T++VRK W ++++ +W+ S W K + R+ LND++RF++R A R + +L+ Sbjct: 7 VQTGRIAKASAGPLKGRLVAIVDVIDQNRVLVDGPLTGVPRQEYRLNNLHLTKYRIKFPYTAPTRIVRKAWTESDLKAQWKVSPWSVKAQNICKRSSLNDFDRFKLRYAKRQRNKLL 123
BLAST of 60s ribosomal protein L14 vs. UniProt/SwissProt
Match: sp|Q95ZE8|RL14_DROVI (60S ribosomal protein L14 OS=Drosophila virilis OX=7244 GN=RpL14 PE=3 SV=1) HSP 1 Score: 119.013 bits (297), Expect = 1.051e-32 Identity = 51/117 (43.59%), Postives = 82/117 (70.09%), Query Frame = 3 Query: 108 VQCGRVAFIATGEDQGKLCAIVEIIDQNRVLIDGPLSGVVRKSVNISNLHLTSFNIHLPHGSRTKVVRKEWLKAEISKKWEESTWCQKLKKKAIRAGLNDYERFQVRVAIRNKTRLI 458 VQ GR+A + G +G+L AIV++IDQNRVL+DGPL+GV R+ ++NLHLT + I P+ + T++VRK W+ +++ +W+ S W K + R+ LND++RF++R A R + +L+ Sbjct: 7 VQTGRIAKASAGPLKGRLVAIVDVIDQNRVLVDGPLTGVPRQEYRLNNLHLTKYRIKFPYTAPTRIVRKAWVDSDLKAQWKVSPWSVKAQNICKRSQLNDFDRFKLRYAKRQRNKLL 123
BLAST of 60s ribosomal protein L14 vs. UniProt/SwissProt
Match: sp|P36105|RL14A_YEAST (60S ribosomal protein L14-A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=RPL14A PE=1 SV=1) HSP 1 Score: 117.857 bits (294), Expect = 1.493e-32 Identity = 53/108 (49.07%), Postives = 72/108 (66.67%), Query Frame = 3 Query: 108 VQCGRVAFIATGEDQGKLCAIVEIIDQNRVLIDGPLSGVVRKSVNISNLHLTSFNIHLPHGSRTKVVRKEWLKAEISKKWEESTWCQKLKKKAIRAGLNDYERFQVRV 431 V+ GRV I G+ GKL AIVEIIDQ +VLIDGP +GV R+++N+ + LT LP G+RT V K+W A + +KW S+W +K+ ++ RA L D+ERFQV V Sbjct: 15 VEVGRVVLIKKGQSAGKLAAIVEIIDQKKVLIDGPKAGVPRQAINLGQVVLTPLTFALPRGARTATVSKKWAAAAVCEKWAASSWAKKIAQRERRAALTDFERFQVMV 122
BLAST of 60s ribosomal protein L14 vs. UniProt/SwissProt
Match: sp|P38754|RL14B_YEAST (60S ribosomal protein L14-B OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=RPL14B PE=1 SV=1) HSP 1 Score: 117.087 bits (292), Expect = 2.677e-32 Identity = 53/108 (49.07%), Postives = 72/108 (66.67%), Query Frame = 3 Query: 108 VQCGRVAFIATGEDQGKLCAIVEIIDQNRVLIDGPLSGVVRKSVNISNLHLTSFNIHLPHGSRTKVVRKEWLKAEISKKWEESTWCQKLKKKAIRAGLNDYERFQVRV 431 V+ GRV I G+ GKL AIVEIIDQ +VLIDGP +GV R+++N+ + LT LP G+RT V K+W A + +KW S+W +K+ ++ RA L D+ERFQV V Sbjct: 15 VEVGRVVLIKKGQSAGKLAAIVEIIDQKKVLIDGPKAGVPRQAINLGQVVLTPLTFALPRGARTATVSKKWAAAGVCEKWAASSWAKKIAQRERRAALTDFERFQVMV 122
BLAST of 60s ribosomal protein L14 vs. TrEMBL
Match: A0A1I8H056 (Ribosomal_L14e domain-containing protein OS=Macrostomum lignano OX=282301 PE=4 SV=1) HSP 1 Score: 138.658 bits (348), Expect = 4.471e-38 Identity = 61/123 (49.59%), Postives = 90/123 (73.17%), Query Frame = 3 Query: 102 KTVQCGRVAFIATGEDQGKLCAIVEIIDQNRVLIDGPLSGVVRKSVNISNLHLTSFNIHLPHGSRTKVVRKEWLKAEISKKWEESTWCQKLKKKAIRAGLNDYERFQVRVAIRNKTRLIAQEM 470 K VQ GRVAFIA G D GK+CAI EI+DQNRVLIDGP +GV R+ ++ LHLT F++ LPH SRT VVR++WL A++ ++W ++W +KL+ + +RA + D++RF+ A + + R+++ M Sbjct: 6 KFVQVGRVAFIANGADSGKVCAIAEIVDQNRVLIDGPCTGVRRQVCDMKRLHLTGFHLPLPHSSRTAVVRRKWLAAKLDERWTGTSWAKKLEARRLRASMTDFDRFRCARAKQIRNRIVSVAM 128
BLAST of 60s ribosomal protein L14 vs. TrEMBL
Match: A0A267GKT1 (Ribosomal_L14e domain-containing protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig029673g3 PE=4 SV=1) HSP 1 Score: 138.658 bits (348), Expect = 4.743e-38 Identity = 61/123 (49.59%), Postives = 90/123 (73.17%), Query Frame = 3 Query: 102 KTVQCGRVAFIATGEDQGKLCAIVEIIDQNRVLIDGPLSGVVRKSVNISNLHLTSFNIHLPHGSRTKVVRKEWLKAEISKKWEESTWCQKLKKKAIRAGLNDYERFQVRVAIRNKTRLIAQEM 470 K VQ GRVAFIA G D GK+CAI EI+DQNRVLIDGP +GV R+ ++ LHLT F++ LPH SRT VVR++WL A++ ++W ++W +KL+ + +RA + D++RF+ A + + R+++ M Sbjct: 4 KFVQVGRVAFIANGADSGKVCAIAEIVDQNRVLIDGPCTGVRRQVCDMKRLHLTGFHLPLPHSSRTAVVRRKWLAAKLDERWTGTSWAKKLEARRLRASMTDFDRFRCARAKQIRNRIVSVAM 126
BLAST of 60s ribosomal protein L14 vs. TrEMBL
Match: A0A1I8HLV3 (Ribosomal_L14e domain-containing protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig029673g2 PE=4 SV=1) HSP 1 Score: 138.272 bits (347), Expect = 5.494e-38 Identity = 61/123 (49.59%), Postives = 90/123 (73.17%), Query Frame = 3 Query: 102 KTVQCGRVAFIATGEDQGKLCAIVEIIDQNRVLIDGPLSGVVRKSVNISNLHLTSFNIHLPHGSRTKVVRKEWLKAEISKKWEESTWCQKLKKKAIRAGLNDYERFQVRVAIRNKTRLIAQEM 470 K VQ GRVAFIA G D GK+CAI EI+DQNRVLIDGP +GV R+ ++ LHLT F++ LPH SRT VVR++WL A++ ++W ++W +KL+ + +RA + D++RF+ A + + R+++ M Sbjct: 6 KFVQVGRVAFIANGADSGKVCAIAEIVDQNRVLIDGPCTGVRRQVCDMKRLHLTGFHLPLPHSSRTAVVRRKWLAAKLDERWTGTSWAKKLEARRLRASMTDFDRFKCARAKQIRNRIVSVAM 128
BLAST of 60s ribosomal protein L14 vs. TrEMBL
Match: A0A1I8IB88 (Ribosomal_L14e domain-containing protein OS=Macrostomum lignano OX=282301 PE=4 SV=1) HSP 1 Score: 138.272 bits (347), Expect = 6.753e-38 Identity = 61/123 (49.59%), Postives = 89/123 (72.36%), Query Frame = 3 Query: 102 KTVQCGRVAFIATGEDQGKLCAIVEIIDQNRVLIDGPLSGVVRKSVNISNLHLTSFNIHLPHGSRTKVVRKEWLKAEISKKWEESTWCQKLKKKAIRAGLNDYERFQVRVAIRNKTRLIAQEM 470 K VQ GRVAFIA G D GK+CAI EI+DQNRVLIDGP +GV R+ ++ LHLT F++ LPH SRT VVR++WL A + ++W ++W +KL+ + +RA + D++RF+ A + + R+++ M Sbjct: 6 KFVQVGRVAFIANGADSGKVCAIAEIVDQNRVLIDGPCTGVRRQVCDMKRLHLTGFHLPLPHSSRTAVVRRKWLAANLDERWTGTSWAKKLEARRLRASMTDFDRFRCARAKQIRNRIVSVAM 128
BLAST of 60s ribosomal protein L14 vs. TrEMBL
Match: A0A267GTU7 (Ribosomal_L14e domain-containing protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig029673g1 PE=4 SV=1) HSP 1 Score: 138.272 bits (347), Expect = 6.843e-38 Identity = 61/123 (49.59%), Postives = 89/123 (72.36%), Query Frame = 3 Query: 102 KTVQCGRVAFIATGEDQGKLCAIVEIIDQNRVLIDGPLSGVVRKSVNISNLHLTSFNIHLPHGSRTKVVRKEWLKAEISKKWEESTWCQKLKKKAIRAGLNDYERFQVRVAIRNKTRLIAQEM 470 K VQ GRVAFIA G D GK+CAI EI+DQNRVLIDGP +GV R+ ++ LHLT F++ LPH SRT VVR++WL A + ++W ++W +KL+ + +RA + D++RF+ A + + R+++ M Sbjct: 5 KFVQVGRVAFIANGADSGKVCAIAEIVDQNRVLIDGPCTGVRRQVCDMKRLHLTGFHLPLPHSSRTAVVRRKWLAANLDERWTGTSWAKKLEARRLRASMTDFDRFRCARAKQIRNRIVSVAM 127
BLAST of 60s ribosomal protein L14 vs. Ensembl Cavefish
Match: rpl14 (ribosomal protein L14 [Source:NCBI gene;Acc:103033049]) HSP 1 Score: 125.946 bits (315), Expect = 1.176e-36 Identity = 55/121 (45.45%), Postives = 86/121 (71.07%), Query Frame = 3 Query: 108 VQCGRVAFIATGEDQGKLCAIVEIIDQNRVLIDGPLSGVVRKSVNISNLHLTSFNIHLPHGSRTKVVRKEWLKAEISKKWEESTWCQKLKKKAIRAGLNDYERFQVRVAIRNKTRLIAQEM 470 V+ GRVA++ G +GKL AIV++IDQNR L+DGP +GV R+S+ + LT + I +PH +R K VR+ W KAE++KKW ES+W +K++ + RA + D++RF+V A + + ++I QE+ Sbjct: 7 VEIGRVAYVGFGPHEGKLVAIVDVIDQNRALVDGPCTGVKRQSMPFKCMQLTDYVIKIPHSARQKFVRRAWEKAEVNKKWSESSWAKKIEARQRRAKMTDFDRFKVMKAKKMRNKIIKQEV 127
BLAST of 60s ribosomal protein L14 vs. Ensembl Cavefish
Match: rpl14 (ribosomal protein L14 [Source:NCBI gene;Acc:103033049]) HSP 1 Score: 125.946 bits (315), Expect = 1.176e-36 Identity = 55/121 (45.45%), Postives = 86/121 (71.07%), Query Frame = 3 Query: 108 VQCGRVAFIATGEDQGKLCAIVEIIDQNRVLIDGPLSGVVRKSVNISNLHLTSFNIHLPHGSRTKVVRKEWLKAEISKKWEESTWCQKLKKKAIRAGLNDYERFQVRVAIRNKTRLIAQEM 470 V+ GRVA++ G +GKL AIV++IDQNR L+DGP +GV R+S+ + LT + I +PH +R K VR+ W KAE++KKW ES+W +K++ + RA + D++RF+V A + + ++I QE+ Sbjct: 7 VEIGRVAYVGFGPHEGKLVAIVDVIDQNRALVDGPCTGVKRQSMPFKCMQLTDYVIKIPHSARQKFVRRAWEKAEVNKKWSESSWAKKIEARQRRAKMTDFDRFKVMKAKKMRNKIIKQEV 127
BLAST of 60s ribosomal protein L14 vs. Ensembl Yeast
Match: RPL14A (Ribosomal 60S subunit protein L14A; N-terminally acetylated; homologous to mammalian ribosomal protein L14, no bacterial homolog; RPL14A has a paralog, RPL14B, that arose from the whole genome duplication [Source:SGD;Acc:S000001489]) HSP 1 Score: 117.857 bits (294), Expect = 2.150e-34 Identity = 53/108 (49.07%), Postives = 72/108 (66.67%), Query Frame = 3 Query: 108 VQCGRVAFIATGEDQGKLCAIVEIIDQNRVLIDGPLSGVVRKSVNISNLHLTSFNIHLPHGSRTKVVRKEWLKAEISKKWEESTWCQKLKKKAIRAGLNDYERFQVRV 431 V+ GRV I G+ GKL AIVEIIDQ +VLIDGP +GV R+++N+ + LT LP G+RT V K+W A + +KW S+W +K+ ++ RA L D+ERFQV V Sbjct: 15 VEVGRVVLIKKGQSAGKLAAIVEIIDQKKVLIDGPKAGVPRQAINLGQVVLTPLTFALPRGARTATVSKKWAAAAVCEKWAASSWAKKIAQRERRAALTDFERFQVMV 122
BLAST of 60s ribosomal protein L14 vs. Ensembl Yeast
Match: RPL14B (Ribosomal 60S subunit protein L14B; homologous to mammalian ribosomal protein L14, no bacterial homolog; RPL14B has a paralog, RPL14A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress [Source:SGD;Acc:S000000993]) HSP 1 Score: 117.087 bits (292), Expect = 3.855e-34 Identity = 53/108 (49.07%), Postives = 72/108 (66.67%), Query Frame = 3 Query: 108 VQCGRVAFIATGEDQGKLCAIVEIIDQNRVLIDGPLSGVVRKSVNISNLHLTSFNIHLPHGSRTKVVRKEWLKAEISKKWEESTWCQKLKKKAIRAGLNDYERFQVRV 431 V+ GRV I G+ GKL AIVEIIDQ +VLIDGP +GV R+++N+ + LT LP G+RT V K+W A + +KW S+W +K+ ++ RA L D+ERFQV V Sbjct: 15 VEVGRVVLIKKGQSAGKLAAIVEIIDQKKVLIDGPKAGVPRQAINLGQVVLTPLTFALPRGARTATVSKKWAAAGVCEKWAASSWAKKIAQRERRAALTDFERFQVMV 122
BLAST of 60s ribosomal protein L14 vs. Ensembl Nematostella
Match: EDO39219 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SAM6]) HSP 1 Score: 120.168 bits (300), Expect = 6.359e-35 Identity = 52/121 (42.98%), Postives = 84/121 (69.42%), Query Frame = 3 Query: 108 VQCGRVAFIATGEDQGKLCAIVEIIDQNRVLIDGPLSGVVRKSVNISNLHLTSFNIHLPHGSRTKVVRKEWLKAEISKKWEESTWCQKLKKKAIRAGLNDYERFQVRVAIRNKTRLIAQEM 470 V+ GRVA I G D+GKLC +V+++DQNR LIDGP + V R+++N+ +L LT F I + +R+ V+K + A++ KWE++ W +KL + RA LND++RF++++A + K RL+ E+ Sbjct: 7 VEVGRVALINAGPDEGKLCVVVDLVDQNRALIDGPCTSVCRQAINMKHLSLTDFKIKIGRSARSGPVKKAFEAAQVQDKWEQTAWARKLAMRKKRATLNDFDRFKLKLAKQKKNRLLRTEV 127
BLAST of 60s ribosomal protein L14 vs. Ensembl Medaka
Match: rpl14 (ribosomal protein L14 [Source:NCBI gene;Acc:101161376]) HSP 1 Score: 119.398 bits (298), Expect = 3.968e-34 Identity = 52/121 (42.98%), Postives = 85/121 (70.25%), Query Frame = 3 Query: 108 VQCGRVAFIATGEDQGKLCAIVEIIDQNRVLIDGPLSGVVRKSVNISNLHLTSFNIHLPHGSRTKVVRKEWLKAEISKKWEESTWCQKLKKKAIRAGLNDYERFQVRVAIRNKTRLIAQEM 470 V+ GRVA+++ G GKL AIV++IDQNR L+DGP +GV R+++ + LT + I +PH +R K VRK W KA++++KW +S W +K++ + RA ++D++RF+V A R + ++I E+ Sbjct: 7 VEIGRVAYLSFGPHAGKLVAIVDVIDQNRALVDGPCTGVKRQAMPFKCMQLTDYVIKVPHSARQKFVRKAWEKAQVNEKWAQSNWAKKIEARQKRAKMSDFDRFKVMKAKRMRNKIIRHEV 127
BLAST of 60s ribosomal protein L14 vs. Planmine SMEST
Match: SMESG000022343.1 (SMESG000022343.1) HSP 1 Score: 265.774 bits (678), Expect = 7.109e-92 Identity = 128/128 (100.00%), Postives = 128/128 (100.00%), Query Frame = 3 Query: 90 MKEVKTVQCGRVAFIATGEDQGKLCAIVEIIDQNRVLIDGPLSGVVRKSVNISNLHLTSFNIHLPHGSRTKVVRKEWLKAEISKKWEESTWCQKLKKKAIRAGLNDYERFQVRVAIRNKTRLIAQEMY 473 MKEVKTVQCGRVAFIATGEDQGKLCAIVEIIDQNRVLIDGPLSGVVRKSVNISNLHLTSFNIHLPHGSRTKVVRKEWLKAEISKKWEESTWCQKLKKKAIRAGLNDYERFQVRVAIRNKTRLIAQEMY Sbjct: 1 MKEVKTVQCGRVAFIATGEDQGKLCAIVEIIDQNRVLIDGPLSGVVRKSVNISNLHLTSFNIHLPHGSRTKVVRKEWLKAEISKKWEESTWCQKLKKKAIRAGLNDYERFQVRVAIRNKTRLIAQEMY 128 The following BLAST results are available for this feature:
BLAST of 60s ribosomal protein L14 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 3
BLAST of 60s ribosomal protein L14 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 2
BLAST of 60s ribosomal protein L14 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 1
BLAST of 60s ribosomal protein L14 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 1
BLAST of 60s ribosomal protein L14 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 2
BLAST of 60s ribosomal protein L14 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 1
BLAST of 60s ribosomal protein L14 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of 60s ribosomal protein L14 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of 60s ribosomal protein L14 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 2
BLAST of 60s ribosomal protein L14 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of 60s ribosomal protein L14 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 2
BLAST of 60s ribosomal protein L14 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 1
BLAST of 60s ribosomal protein L14 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 1
BLAST of 60s ribosomal protein L14 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 1
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30023172 ID=SMED30023172|Name=60s ribosomal protein L14|organism=Schmidtea mediterranea sexual|type=transcript|length=639bpback to top protein sequence of SMED30023172-orf-1 >SMED30023172-orf-1 ID=SMED30023172-orf-1|Name=SMED30023172-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=139bp MKEVKTVQCGRVAFIATGEDQGKLCAIVEIIDQNRVLIDGPLSGVVRKSVback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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