Tryptophan--tRNA ligase

Overview
NameTryptophan--tRNA ligase
Smed IDSMED30023152
Length (bp)1278
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Tryptophan--tRNA ligase (SMED30023152) t-SNE clustered cells

Violin plots show distribution of expression levels for Tryptophan--tRNA ligase (SMED30023152) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Tryptophan--tRNA ligase (SMED30023152) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Tryptophan--tRNA ligase (SMED30023152) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30023152

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 1

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
parenchymaSMED30023152h1SMcG0001333 dd_Smed_v4_6466_0_1dd_Smed_v4PMID:27063937
Scimone et al., 2016		whole organism asexual adult colorimetric in situ hybridization evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Tryptophan--tRNA ligase vs. Ensembl Human
Match: WARS2 (tryptophanyl tRNA synthetase 2, mitochondrial [Source:HGNC Symbol;Acc:HGNC:12730])

HSP 1 Score: 221.476 bits (563), Expect = 6.177e-68
Identity = 123/338 (36.39%), Postives = 191/338 (56.51%), Query Frame = 3 
Query:  192 KTVMSGIQPTGIPHLGNYYGAINQWIKLKSNPNVEQIFISIVDLHSITSYDKLNNVNENVYLMAASLMACGLSGKKVSLFQQSNIINHTYLAWILMCICPLSKIQAMPQWKEKLESWPGDELPNSGLLMYPVLQAADILLYAPELVPVGEDQIIHLELARYLRNKLINWHRFSPDCELSKYLPLPEMMKCRFPKIYNLKNCKKKMSKSDENGYSRIELMDSPDQIRNKIMKSQTDAFSRNITYDISERPALANLINIYCSVKDSELEGAMFEIEKLSKIQFKELLVDELIQLIDPIRLKIIDLLNNRDEIDRVLKAGHEIANQKANETINIVAHHIGL 1205
            K V SGIQPTGI HLGNY GAI  W++L+     + +  SIVDLHSIT       + +++  M A L+ACG++ +K  LFQQS +  HT L+WIL C+  L ++Q + QWK K      D     GLL YPVLQAADILLY    VPVGEDQ+ H+EL + L            + +  ++ P+PE +     K+ +L++   KMSKSD +  + + + DSP++I  K  K+ TD F+  +TYD + R  ++N++ ++ +V    +E  +     ++  ++K  + D +I+   PI+ +I  L  ++D +++VL+ G   A + A      V   +G 
Sbjct:   35 KRVFSGIQPTGILHLGNYLGAIESWVRLQDE--YDSVLYSIVDLHSITVPQDPAVLRQSILDMTAVLLACGINPEKSILFQQSQVSEHTQLSWILSCMVRLPRLQHLHQWKAKTTKQKHD--GTVGLLTYPVLQAADILLYKSTHVPVGEDQVQHMELVQDLAQGF--------NKKYGEFFPVPESILTSMKKVKSLRDPSAKMSKSDPDKLATVRITDSPEEIVQKFRKAVTD-FTSEVTYDPAGRAGVSNIVAVHAAVTGLSVEEVVRRSAGMNTARYKLAVADAVIEKFAPIKREIEKLKLDKDHLEKVLQIGSAKAKELAYTVCQEVKKLVGF 359
[Close]
BLAST of Tryptophan--tRNA ligase vs. Ensembl Human
Match: WARS2 (tryptophanyl tRNA synthetase 2, mitochondrial [Source:HGNC Symbol;Acc:HGNC:12730])

HSP 1 Score: 153.68 bits (387), Expect = 1.088e-43
Identity = 84/186 (45.16%), Postives = 111/186 (59.68%), Query Frame = 3 
Query:  192 KTVMSGIQPTGIPHLGNYYGAINQWIKLKSNPNVEQIFISIVDLHSITSYDKLNNVNENVYLMAASLMACGLSGKKVSLFQQSNIINHTYLAWILMCICPLSKIQAMPQWKEKLESWPGDELPNSGLLMYPVLQAADILLYAPELVPVGEDQIIHLELARYLRNKLINWHRFSPDCELSKYLPLPE 749
            K V SGIQPTGI HLGNY GAI  W++L+     + +  SIVDLHSIT       + +++  M A L+ACG++ +K  LFQQS +  HT L+WIL C+  L ++Q + QWK K      D     GLL YPVLQAADILLY    VPVGEDQ+ H+EL + L            + +  ++ P+PE
Sbjct:   35 KRVFSGIQPTGILHLGNYLGAIESWVRLQDE--YDSVLYSIVDLHSITVPQDPAVLRQSILDMTAVLLACGINPEKSILFQQSQVSEHTQLSWILSCMVRLPRLQHLHQWKAKTTKQKHDG--TVGLLTYPVLQAADILLYKSTHVPVGEDQVQHMELVQDLAQGF--------NKKYGEFFPVPE 208
[Close]
BLAST of Tryptophan--tRNA ligase vs. Ensembl Celegans
Match: prx-10 (Putative peroxisomal biogenesis factor 10 [Source:UniProtKB/Swiss-Prot;Acc:C0HKD7])

HSP 1 Score: 189.504 bits (480), Expect = 4.373e-56
Identity = 119/339 (35.10%), Postives = 194/339 (57.23%), Query Frame = 3 
Query:  201 MSGIQPTGIPHLGNYYGAINQWIKLKSNPNVE-QIFISIVDLHSITSYDKL--NNVNENVYLMAASLMACGLSGKKVSLFQQSNIINHTYLAWILMCICPLSKIQAMPQWKEKLESWPGDELPNSGLLMYPVLQAADILLYAPELVPVGEDQIIHLELARYLRNKLINWHRFSPDCELSKYLPLP-EMMKCRFPKIYNLKNCKKKMSKSDENGYSRIELMDSPDQIRNKIMKSQTDAFSRNITYDISERPALANLINIYCSVKDSELEGAMFEIEKLSKIQFKELLVDELIQLIDPIRLKIIDLLNNRDEIDRVLKAGHEIANQKANETINIVAHHIGL 1205
             +GIQPTGIPHLGN++G+I  W +L+++ +    + +S+VD H+I S   L  N + +N + M ASL+ACG+   +  LF+QS++     ++WIL  +   SK+  +PQ+KEK E +   ++P  GLL YP+LQAAD+L +    VPVGEDQ  HL L   L       + F+   E ++  P+P ++ +    +I +L+  +KKMSKS     SRIE+ DS   I  K  K+Q+D   + +TYD   R A++NL+++Y +V  ++ + +  +    + +  K  L + + + + PIR K  + L N  E+D+VL    E A + A + +  +   IG 
Sbjct:   34 FTGIQPTGIPHLGNFFGSIEPWTELQNSVDKNILMMLSVVDQHAI-SLGPLPANELRQNTHQMTASLIACGVDPNRTLLFRQSDVPQIAQISWILGSLQTTSKLARLPQYKEKKERFKKGDIP-VGLLTYPLLQAADVLTFKATTVPVGEDQSQHLNLLGGL------AYAFNKTYE-TEIFPIPKQLTRESHARIRSLREPEKKMSKSSGGPRSRIEITDSRSTIIEKCQKAQSDNAGK-VTYDKENRLAVSNLLDLYSAV--TKTQTSEIDFSNWTTLDLKMNLAEAVDKRLAPIRQK-FEELQNTGEVDKVLTENGEKAREIAEKNLEEIRRTIGF 359
[Close]
BLAST of Tryptophan--tRNA ligase vs. Ensembl Fly
Match: TrpRS-m (gene:FBgn0036763 transcript:FBtr0075141)

HSP 1 Score: 219.164 bits (557), Expect = 1.979e-64
Identity = 126/345 (36.52%), Postives = 203/345 (58.84%), Query Frame = 3 
Query:  192 KTVMSGIQPTGIPHLGNYYGAINQWIKLKSNPNVEQIFISIVDLHSITSYDKLNNVNENVYLMAASLMACGLSGKKVSLFQQSNIINHTYLAWILMCICPLSKIQAMPQWKEK---LESWPGDELPNSGLLMYPVLQAADILLYAPELVPVGEDQIIHLELARYLRNKLINWHRFSPDCELSKYLPLPEMMKCRFPKIYNLKNCKKKMSKSDENGYSRIELMDSPDQIRNKIMKSQTDAFSRNITYDISERPALANLINIYCSVKDSELEGAMFEIEKLSKIQFKELLVDELIQLIDPIRLKIIDLLNNRDEIDRVLKAGHEIANQKANETINIVAHHIGLKSHA 1217
            + V SGIQPTG  HLGNY GA+ +W++L++    + + + IVDLHSIT       + EN++ MAA+L+ACG+   K +LF QS +  H    WIL  +  + ++  +PQ++EK   L+  P       GL +YPVLQAADI+LY    VPVG DQI H++LA++L  ++ N  R+     +   +    +      ++ +L++  KKMSKS+ N  + I L DSPD I  KI K+ TD F+ +ITY+  +R  ++NL+NI+  V    ++  + E   L   ++K+ + + +++ + PIR +I   +  R+E+  +L+ G E A Q+A +T+N V   +GL + A
Sbjct:   80 RKVFSGIQPTGSLHLGNYLGAVRKWVQLQNA--RDDVTVCIVDLHSITMPHNPPLLRENIFTMAATLLACGIDPTKSTLFVQSAVAEHAEFNWILSSLTTMPRLAQLPQFREKSRLLKDVP------LGLYVYPVLQAADIMLYKSTHVPVGADQIQHIQLAQHL-ARIYNG-RYGETFPVCTAI----IEDGDASRVLSLRDPSKKMSKSEANPKATINLCDSPDLITQKIKKAVTD-FTSDITYNPGKRAGVSNLVNIHAQVTGQSIKTVVNEAATLDTAKYKDRVAEAVVEHLRPIREQIHHHMTKRNEMIYLLEVGAEKARQQARQTLNDVKQRLGLGTSA 409
[Close]
BLAST of Tryptophan--tRNA ligase vs. Ensembl Fly
Match: TrpRS-m (gene:FBgn0036763 transcript:FBtr0331811)

HSP 1 Score: 219.164 bits (557), Expect = 1.979e-64
Identity = 126/345 (36.52%), Postives = 203/345 (58.84%), Query Frame = 3 
Query:  192 KTVMSGIQPTGIPHLGNYYGAINQWIKLKSNPNVEQIFISIVDLHSITSYDKLNNVNENVYLMAASLMACGLSGKKVSLFQQSNIINHTYLAWILMCICPLSKIQAMPQWKEK---LESWPGDELPNSGLLMYPVLQAADILLYAPELVPVGEDQIIHLELARYLRNKLINWHRFSPDCELSKYLPLPEMMKCRFPKIYNLKNCKKKMSKSDENGYSRIELMDSPDQIRNKIMKSQTDAFSRNITYDISERPALANLINIYCSVKDSELEGAMFEIEKLSKIQFKELLVDELIQLIDPIRLKIIDLLNNRDEIDRVLKAGHEIANQKANETINIVAHHIGLKSHA 1217
            + V SGIQPTG  HLGNY GA+ +W++L++    + + + IVDLHSIT       + EN++ MAA+L+ACG+   K +LF QS +  H    WIL  +  + ++  +PQ++EK   L+  P       GL +YPVLQAADI+LY    VPVG DQI H++LA++L  ++ N  R+     +   +    +      ++ +L++  KKMSKS+ N  + I L DSPD I  KI K+ TD F+ +ITY+  +R  ++NL+NI+  V    ++  + E   L   ++K+ + + +++ + PIR +I   +  R+E+  +L+ G E A Q+A +T+N V   +GL + A
Sbjct:   80 RKVFSGIQPTGSLHLGNYLGAVRKWVQLQNA--RDDVTVCIVDLHSITMPHNPPLLRENIFTMAATLLACGIDPTKSTLFVQSAVAEHAEFNWILSSLTTMPRLAQLPQFREKSRLLKDVP------LGLYVYPVLQAADIMLYKSTHVPVGADQIQHIQLAQHL-ARIYNG-RYGETFPVCTAI----IEDGDASRVLSLRDPSKKMSKSEANPKATINLCDSPDLITQKIKKAVTD-FTSDITYNPGKRAGVSNLVNIHAQVTGQSIKTVVNEAATLDTAKYKDRVAEAVVEHLRPIREQIHHHMTKRNEMIYLLEVGAEKARQQARQTLNDVKQRLGLGTSA 409
[Close]
BLAST of Tryptophan--tRNA ligase vs. Ensembl Zebrafish
Match: wars2 (tryptophanyl tRNA synthetase 2, mitochondrial [Source:ZFIN;Acc:ZDB-GENE-050306-26])

HSP 1 Score: 232.646 bits (592), Expect = 1.761e-72
Identity = 134/355 (37.75%), Postives = 202/355 (56.90%), Query Frame = 3 
Query:  141 RIFYFSKLYTNVACKRFKTVMSGIQPTGIPHLGNYYGAINQWIKLKSNPNVEQIFISIVDLHSITSYDKLNNVNENVYLMAASLMACGLSGKKVSLFQQSNIINHTYLAWILMCICPLSKIQAMPQWKEKLESWPGDELPNSGLLMYPVLQAADILLYAPELVPVGEDQIIHLELARYLRNKLINWHRFSPDCELSKYLPLPEMMKCRFPKIYNLKNCKKKMSKSDENGYSRIELMDSPDQIRNKIMKSQTDAFSRNITYDISERPALANLINIYCSVKDSELEGAMFEIEKLSKIQFKELLVDELIQLIDPIRLKIIDLLNNRDEIDRVLKAGHEIANQKANETINIVAHHIGL 1205
            R+F   K  T    +    V SGIQPTGIPHLGNY GA+  W+ L+ + +   +  SIVDLHSIT       + +N+  MAASL+ACG+   +  LFQQS +  H  L+WIL C+  + +++ +PQW  K++S   +E  + GL  YPVLQAADILLY    VPVGEDQI HLELA+ L     N        +  ++ P P  +     K+ +L++   KMSKSD   +S + L D+PD I  KI ++ TD F+  +TY+   RP ++NL++++ +V    +EG +   E +   Q+K ++ + +I+ + PIRL+I  L  ++  ++ +L  G E A   A   +  V   +G 
Sbjct:   24 RLFKAGKA-TASTSRSKGLVFSGIQPTGIPHLGNYLGALESWVALQDDYST--VMYSIVDLHSITQPQDPQVLRDNIMDMAASLLACGIDPTRSILFQQSQVSEHAELSWILGCLTSMPRLRHLPQW--KMKSKQKNE-GSVGLYTYPVLQAADILLYKSTHVPVGEDQIQHLELAQDLARIFNN--------QYGEFFPEPRALLSGTRKVKSLRDPSSKMSKSDPQRFSAVFLTDTPDDIALKIRRAVTD-FTSEVTYEPDTRPGVSNLVSMHAAVSGQTIEGVIRLAEGMDTGQYKNMVAEAIIKKLQPIRLEIQRLRADQGYLESLLAQGAEKARSLAAPVMKEVRKLLGF 363
[Close]
BLAST of Tryptophan--tRNA ligase vs. Ensembl Xenopus
Match: WARS2 (tryptophanyl tRNA synthetase 2, mitochondrial [Source:NCBI gene;Acc:101731027])

HSP 1 Score: 170.629 bits (431), Expect = 1.202e-49
Identity = 100/277 (36.10%), Postives = 154/277 (55.60%), Query Frame = 3 
Query:  378 MAASLMACGLSGKKVSLFQQSNIINHTYLAWILMCICPLSKIQAMPQWKEKLESWPGDELPNSGLLMYPVLQAADILLYAPELVPVGEDQIIHLELARYLRNKLINWHRFSPDCELSKYLPLPEMMKCRFPKIYNLKNCKKKMSKSDENGYSRIELMDSPDQIRNKIMKSQTDAFSRNITYDISERPALANLINIYCSVKDSELEGAMFEIEKLSKIQFKELLVDELIQLIDPIRLKIIDLLNNRDEIDRVLKAGHEIANQKANETINIVAHHIGLK 1208
            MAA L+ACG+S  +  +FQQS +  H  LAWIL C+  + +++ +PQWK K  S   +   + GL  YPVLQAADILLY    VPVG+DQ+ HLEL +    +L N  R   D     + P+P+ +     KI +L++   KMSKSD +  + + L DSP++I  K  K+ TD F+  +TYD   RP ++NL+ I+ +V     E  +     +   Q+K  + + +IQ + PIR ++  L  +R  +  VL+ G + A + A    + V   +G +
Sbjct:    1 MAACLLACGISPAQSCIFQQSQVAEHAELAWILSCLTSMPRLRHLPQWKVK--SQRQNNEGSVGLFTYPVLQAADILLYRATHVPVGDDQVQHLELTQDT-ARLFN--RLYGD-----FFPIPKAIMGTSKKIRSLRDPSSKMSKSDPHKLATVRLTDSPEEIVMKFRKAVTD-FTSEVTYDPEGRPGVSNLVAIHSAVTGLSAEEIVGRSRGMETAQYKLAVAEAVIQKLRPIREEMHRLQKDRGYLQGVLRQGAQRAKEMAAPVYDSVCRLVGFR 266
[Close]
BLAST of Tryptophan--tRNA ligase vs. Ensembl Xenopus
Match: WARS2 (tryptophanyl tRNA synthetase 2, mitochondrial [Source:NCBI gene;Acc:101731027])

HSP 1 Score: 147.517 bits (371), Expect = 8.804e-41
Identity = 91/273 (33.33%), Postives = 146/273 (53.48%), Query Frame = 3 
Query:  393 MACGLSGKKVSLFQQSN-IINHTYLAWILMCICPLSKIQAMPQWKEKLESWPGDELPNSGLLMYPVLQAADILLYAPELVPVGEDQIIHLELARYLRNKLINWHRFSPDCELSKYLPLPEMMKCRFPKIYNLKNCKKKMSKSDENGYSRIELMDSPDQIRNKIMKSQTDAFSRNITYDISERPALANLINIYCSVKDSELEGAMFEIEKLSKIQFKELLVDELIQLIDPIRLKIIDLLNNRDEIDRVLKAGHEIANQKANETINIVAHHIGLK 1208
            +  G  G + S  ++ + +  H  LAWIL C+  + +++ +PQWK K  S   +   + GL  YPVLQAADILLY    VPVG+DQ+ HLEL +    +L N  R   D     + P+P+ +     KI +L++   KMSKSD +  + + L DSP++I  K  K+ TD F+  +TYD   RP ++NL+ I+ +V     E  +     +   Q+K  + + +IQ + PIR ++  L  +R  +  VL+ G + A + A    + V   +G +
Sbjct:   17 LGSGFRGFRESGKERGDQVAEHAELAWILSCLTSMPRLRHLPQWKVK--SQRQNNEGSVGLFTYPVLQAADILLYRATHVPVGDDQVQHLELTQDT-ARLFN--RLYGD-----FFPIPKAIMGTSKKIRSLRDPSSKMSKSDPHKLATVRLTDSPEEIVMKFRKAVTD-FTSEVTYDPEGRPGVSNLVAIHSAVTGLSAEEIVGRSRGMETAQYKLAVAEAVIQKLRPIREEMHRLQKDRGYLQGVLRQGAQRAKEMAAPVYDSVCRLVGFR 278
[Close]
BLAST of Tryptophan--tRNA ligase vs. Ensembl Mouse
Match: Wars2 (tryptophanyl tRNA synthetase 2 (mitochondrial) [Source:MGI Symbol;Acc:MGI:1917810])

HSP 1 Score: 219.164 bits (557), Expect = 3.482e-67
Identity = 124/336 (36.90%), Postives = 187/336 (55.65%), Query Frame = 3 
Query:  198 VMSGIQPTGIPHLGNYYGAINQWIKLKSNPNVEQIFISIVDLHSITSYDKLNNVNENVYLMAASLMACGLSGKKVSLFQQSNIINHTYLAWILMCICPLSKIQAMPQWKEKLESWPGDELPNSGLLMYPVLQAADILLYAPELVPVGEDQIIHLELARYLRNKLINWHRFSPDCELSKYLPLPEMMKCRFPKIYNLKNCKKKMSKSDENGYSRIELMDSPDQIRNKIMKSQTDAFSRNITYDISERPALANLINIYCSVKDSELEGAMFEIEKLSKIQFKELLVDELIQLIDPIRLKIIDLLNNRDEIDRVLKAGHEIANQKANETINIVAHHIGL 1205
            V SGIQPTGI HLGNY GAI  W+ L+     + +  SIVDLHSIT       + +++  M A L+ACG++ +K  LFQQS +  HT L+WIL C+  L ++Q + QWK K      D     GLL YPVLQAADIL Y    VPVGEDQ+ H+EL + L            + +  ++ PLP+ +     K+ +L++   KMSKSD +  + + + DSP++I  K  K+ TD F+  +TY+   R  ++N++ I+ +V    +E  +     L   ++K L+ D +I+   PIR +I  L  ++D + +VL  G   A + A+     V   +G+
Sbjct:   37 VFSGIQPTGILHLGNYLGAIESWVNLQEE--YDTVIYSIVDLHSITVPQDPTVLQQSILDMTAVLLACGINPEKSILFQQSKVSEHTQLSWILTCMVRLPRLQHLHQWKAKAAKQKHDG--TVGLLTYPVLQAADILCYKSTHVPVGEDQVQHMELVQDLARSF--------NQKYGEFFPLPKSILTSMKKVKSLRDPSSKMSKSDPDKLATVRITDSPEEIVQKFRKAVTD-FTSEVTYEPDSRAGVSNMVAIHAAVSGLSVEEVVRSSAGLDTARYKLLVADAVIEKFAPIRKEIEKLKMDKDHLRKVLLVGSAKAKELASPVFEEVKKLVGI 359
[Close]
BLAST of Tryptophan--tRNA ligase vs. Ensembl Mouse
Match: Wars2 (tryptophanyl tRNA synthetase 2 (mitochondrial) [Source:MGI Symbol;Acc:MGI:1917810])

HSP 1 Score: 127.102 bits (318), Expect = 3.585e-34
Identity = 68/139 (48.92%), Postives = 86/139 (61.87%), Query Frame = 3 
Query:  198 VMSGIQPTGIPHLGNYYGAINQWIKLKSNPNVEQIFISIVDLHSITSYDKLNNVNENVYLMAASLMACGLSGKKVSLFQQSNIINHTYLAWILMCICPLSKIQAMPQWKEKLESWPGDELPNSGLLMYPVLQAADILLY 614
            V SGIQPTGI HLGNY GAI  W+ L+     + +  SIVDLHSIT       + +++  M A L+ACG++ +K  LFQQS +  HT L+WIL C+  L ++Q + QWK K      D     GLL YPVLQAADIL Y
Sbjct:   37 VFSGIQPTGILHLGNYLGAIESWVNLQEE--YDTVIYSIVDLHSITVPQDPTVLQQSILDMTAVLLACGINPEKSILFQQSKVSEHTQLSWILTCMVRLPRLQHLHQWKAKAAKQKHDG--TVGLLTYPVLQAADILCY 171
[Close]
BLAST of Tryptophan--tRNA ligase vs. Ensembl Mouse
Match: Wars2 (tryptophanyl tRNA synthetase 2 (mitochondrial) [Source:MGI Symbol;Acc:MGI:1917810])

HSP 1 Score: 126.331 bits (316), Expect = 4.005e-34
Identity = 69/143 (48.25%), Postives = 87/143 (60.84%), Query Frame = 3 
Query:  198 VMSGIQPTGIPHLGNYYGAINQWIKLKSNPNVEQIFISIVDLHSITSYDKLNNVNENVYLMAASLMACGLSGKKVSLFQQSNIINHTYLAWILMCICPLSKIQAMPQWKEKLESWPGDELPNSGLLMYPVLQAADILLYAPEL 626
            V SGIQPTGI HLGNY GAI  W+ L+     + +  SIVDLHSIT       + +++  M A L+ACG++ +K  LFQQS +  HT L+WIL C+  L ++Q + QWK K      D     GLL YPVLQAADIL Y   L
Sbjct:   37 VFSGIQPTGILHLGNYLGAIESWVNLQEE--YDTVIYSIVDLHSITVPQDPTVLQQSILDMTAVLLACGINPEKSILFQQSKVSEHTQLSWILTCMVRLPRLQHLHQWKAKAAKQKHDG--TVGLLTYPVLQAADILCYKVSL 175
[Close]
BLAST of Tryptophan--tRNA ligase vs. Ensembl Mouse
Match: Wars2 (tryptophanyl tRNA synthetase 2 (mitochondrial) [Source:MGI Symbol;Acc:MGI:1917810])

HSP 1 Score: 55.4546 bits (132), Expect = 2.875e-9
Identity = 28/54 (51.85%), Postives = 33/54 (61.11%), Query Frame = 3 
Query:  453 HTYLAWILMCICPLSKIQAMPQWKEKLESWPGDELPNSGLLMYPVLQAADILLY 614
            HT L+WIL C+  L ++Q + QWK K      D     GLL YPVLQAADIL Y
Sbjct:   34 HTQLSWILTCMVRLPRLQHLHQWKAKAAKQKHD--GTVGLLTYPVLQAADILCY 85
[Close]
BLAST of Tryptophan--tRNA ligase vs. UniProt/SwissProt
Match: sp|Q3T099|SYWM_BOVIN (Tryptophan--tRNA ligase, mitochondrial OS=Bos taurus OX=9913 GN=WARS2 PE=2 SV=1)

HSP 1 Score: 225.713 bits (574), Expect = 7.325e-69
Identity = 125/340 (36.76%), Postives = 194/340 (57.06%), Query Frame = 3 
Query:  192 KTVMSGIQPTGIPHLGNYYGAINQWIKLKSNPNVEQIFISIVDLHSITSYDKLNNVNENVYLMAASLMACGLSGKKVSLFQQSNIINHTYLAWILMCICPLSKIQAMPQWKEKLESWPGDELPNS--GLLMYPVLQAADILLYAPELVPVGEDQIIHLELARYLRNKLINWHRFSPDCELSKYLPLPEMMKCRFPKIYNLKNCKKKMSKSDENGYSRIELMDSPDQIRNKIMKSQTDAFSRNITYDISERPALANLINIYCSVKDSELEGAMFEIEKLSKIQFKELLVDELIQLIDPIRLKIIDLLNNRDEIDRVLKAGHEIANQKANETINIVAHHIGL 1205
            K + SGIQPTGIPHLGNY GAI  W++L+   +   +  SIVDLHSIT       + +++  M A+L+ACG++ +K  LFQQS +  HT L+WIL C+  L ++Q + QWK K       E  N   GLL YPVLQAADILLY    VPVGEDQ+ H+EL + L            + +  ++ P+P+ +     K+ +L++   KMSKSD +  + + + DSP++I  K  K+ TD F+  +TY+ + R  ++NL+ I+ +V    +E  +     +   ++K ++ D +I+   PI+ +I  L  N+D +++VL+ G   A + A      V   +G 
Sbjct:   35 KRIFSGIQPTGIPHLGNYLGAIESWVRLQDEHD--SVLYSIVDLHSITVPQDPTILRQSILDMTAALLACGINPEKSILFQQSQVSEHTQLSWILTCMVRLPRLQHLHQWKAKT----AKEKHNGTVGLLTYPVLQAADILLYKSTHVPVGEDQVQHMELVQDLAQSF--------NKKYGEFFPVPKSILTSMKKVKSLRDPSAKMSKSDPDKLATVRITDSPEEIVQKFRKAMTD-FTSEVTYEPASRGGVSNLVAIHAAVAGLPVEEVVRRSAGMDTARYKLVVADAVIEKFAPIKSEIEKLKMNKDHLEKVLQVGSARAKELAYPVCQEVKKLVGF 359
[Close]
BLAST of Tryptophan--tRNA ligase vs. UniProt/SwissProt
Match: sp|Q7VBM9|SYW_PROMA (Tryptophan--tRNA ligase OS=Prochlorococcus marinus (strain SARG / CCMP1375 / SS120) OX=167539 GN=trpS PE=3 SV=1)

HSP 1 Score: 224.557 bits (571), Expect = 1.005e-68
Identity = 137/338 (40.53%), Postives = 205/338 (60.65%), Query Frame = 3 
Query:  192 KTVMSGIQPTGIPHLGNYYGAINQWIKLKSNPNVEQIFISIVDLHSITSYDKLNNVNENVYLMAASLMACGLSGKKVSLFQQSNIINHTYLAWILMCICPLSKIQAMPQWKEKLESWPGDELPNSGLLMYPVLQAADILLYAPELVPVGEDQIIHLELARYLRNKLINWHRFSPDCELSKYLPLPEMMKCRFPKIYNLKNCKKKMSKSDENGYSRIELMDSPDQIRNKIMKSQTDAFSRNITYDISERPALANLINIYCSVKDSELEGAMFEIEKLSKIQFKELLVDELIQLIDPIRLKIIDLLNNRDEIDRVLKAGHEIANQKANETINIVAHHIGL 1205
            K V+SG+QPTG  H+GN+ GAI  W+ L+   N    ++ +VDLH+IT       + EN    AA  +ACG+  KK S+F QS+I  H+ L W+L C+ PL+ ++ M Q+KEK     GD + + GLL YPVL AADILLY  +LVPVGEDQ  HLELAR +  + +N  RF+ + +    +P P +MK    KI +L +   KMSKSD N  SRI L+DSP+ I+ KI +++TD+F   + +D ++RP   NL+ IY  V +   E    E   +   +FK +L D +I+ ++PI+ K   L+ ++ E++ +L  G+  AN  +N+T+  V + +G 
Sbjct:    4 KRVLSGVQPTGAIHIGNWLGAIRNWVSLQ---NEYDTYVCVVDLHAITVPHDPQQLKENTLRTAALYVACGMDPKKCSIFVQSHISAHSELCWLLNCVTPLNWMERMIQFKEKAIK-QGDNV-SIGLLDYPVLMAADILLYDADLVPVGEDQKQHLELARDIAQQRVN-SRFNKESKSILKIPKPLIMK-EGGKIMSLIDGNMKMSKSDPNENSRIALLDSPEIIKKKIKRAKTDSF-LGLEFDNNQRPEANNLLGIYSMVSNQNREAVQKEFSNIGWGKFKPILTDAIIESLNPIQQKYYSLIKDKTELNNILNKGYIKANTISNQTLKRVRNALGF 333
[Close]
BLAST of Tryptophan--tRNA ligase vs. UniProt/SwissProt
Match: sp|Q92HR1|SYW_RICCN (Tryptophan--tRNA ligase OS=Rickettsia conorii (strain ATCC VR-613 / Malish 7) OX=272944 GN=trpS PE=3 SV=1)

HSP 1 Score: 223.016 bits (567), Expect = 2.888e-68
Identity = 135/339 (39.82%), Postives = 203/339 (59.88%), Query Frame = 3 
Query:  192 KTVMSGIQPTGIPHLGNYYGAINQWIKLKSNPNVEQIFISIVDLHSITSYDKLNNVNENVYLMAASLMACGLSGKKVSLFQQSNIINHTYLAWILMCICPLSKIQAMPQWKEKLESWPGDELPNSGLLMYPVLQAADILLYAPELVPVGEDQIIHLELARYLRNKLINWHRFSPDCELSKYLPLPEMMKCRF-PKIYNLKNCKKKMSKSDENGYSRIELMDSPDQIRNKIMKSQTDAFSRNITYDISERPALANLINIYCSVKDSELEGAMFEIEKLSKIQFKELLVDELIQLIDPIRLKIIDLLNNRDEIDRVLKAGHEIANQKANETINIVAHHIGL 1205
            KTV+SG+Q TG  HLGNY GAI  W+K++   N    F  + DLH+IT   K + +N ++  + A  +A GL+  KV++F QS +  HT LAW+L C+ PL  ++ M Q+K+K  S    E    GL  YPVL AADIL+Y  ++VPVGEDQ  HLEL R +   +IN  +F+ +      L +PE++      +I +L++  KKMSKSD + +SRI L D  D I  KI K++TD  S  ++YD   RP ++NL++IY S+ +  LE  +   +     +FKE L + +I  + PIR K ++L+N+++ + ++L  G E A  +A+ET+N V    G 
Sbjct:    3 KTVLSGVQATGSLHLGNYLGAIKNWVKMQEEYNC---FFFLADLHAITVDIKPSELNNSIMEVLAVYLAAGLNPDKVTIFAQSMVKEHTELAWLLNCVTPLGWLKRMTQFKDKAGS--DQEKACLGLFSYPVLMAADILIYKADIVPVGEDQKQHLELTRDIAG-VIN-RKFNKEI-----LKVPEVLISETGTRIMSLRDGLKKMSKSDISDFSRINLKDDNDLIHQKIKKAKTDHLSF-VSYDQETRPEISNLLDIYSSLSEESLEQIIGNYQNQGFAKFKEDLAEIIITNLQPIRDKYLELMNDKEYLLKILHKGAEKARIRASETVNEVKEQFGF 328
[Close]
BLAST of Tryptophan--tRNA ligase vs. UniProt/SwissProt
Match: sp|Q9UGM6|SYWM_HUMAN (Tryptophan--tRNA ligase, mitochondrial OS=Homo sapiens OX=9606 GN=WARS2 PE=1 SV=1)

HSP 1 Score: 221.476 bits (563), Expect = 2.966e-67
Identity = 123/338 (36.39%), Postives = 191/338 (56.51%), Query Frame = 3 
Query:  192 KTVMSGIQPTGIPHLGNYYGAINQWIKLKSNPNVEQIFISIVDLHSITSYDKLNNVNENVYLMAASLMACGLSGKKVSLFQQSNIINHTYLAWILMCICPLSKIQAMPQWKEKLESWPGDELPNSGLLMYPVLQAADILLYAPELVPVGEDQIIHLELARYLRNKLINWHRFSPDCELSKYLPLPEMMKCRFPKIYNLKNCKKKMSKSDENGYSRIELMDSPDQIRNKIMKSQTDAFSRNITYDISERPALANLINIYCSVKDSELEGAMFEIEKLSKIQFKELLVDELIQLIDPIRLKIIDLLNNRDEIDRVLKAGHEIANQKANETINIVAHHIGL 1205
            K V SGIQPTGI HLGNY GAI  W++L+     + +  SIVDLHSIT       + +++  M A L+ACG++ +K  LFQQS +  HT L+WIL C+  L ++Q + QWK K      D     GLL YPVLQAADILLY    VPVGEDQ+ H+EL + L            + +  ++ P+PE +     K+ +L++   KMSKSD +  + + + DSP++I  K  K+ TD F+  +TYD + R  ++N++ ++ +V    +E  +     ++  ++K  + D +I+   PI+ +I  L  ++D +++VL+ G   A + A      V   +G 
Sbjct:   35 KRVFSGIQPTGILHLGNYLGAIESWVRLQDE--YDSVLYSIVDLHSITVPQDPAVLRQSILDMTAVLLACGINPEKSILFQQSQVSEHTQLSWILSCMVRLPRLQHLHQWKAKTTKQKHD--GTVGLLTYPVLQAADILLYKSTHVPVGEDQVQHMELVQDLAQGF--------NKKYGEFFPVPESILTSMKKVKSLRDPSAKMSKSDPDKLATVRITDSPEEIVQKFRKAVTD-FTSEVTYDPAGRAGVSNIVAVHAAVTGLSVEEVVRRSAGMNTARYKLAVADAVIEKFAPIKREIEKLKLDKDHLEKVLQIGSAKAKELAYTVCQEVKKLVGF 359
[Close]
BLAST of Tryptophan--tRNA ligase vs. UniProt/SwissProt
Match: sp|Q8YXE4|SYW_NOSS1 (Tryptophan--tRNA ligase OS=Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576) OX=103690 GN=trpS PE=3 SV=1)

HSP 1 Score: 220.32 bits (560), Expect = 3.489e-67
Identity = 132/340 (38.82%), Postives = 199/340 (58.53%), Query Frame = 3 
Query:  192 KTVMSGIQPTGIPHLGNYYGAINQWIKLKSNPNVEQIFISIVDLHSITSYDKLNNVNENVYLMAASLMACGLSGKKVSLFQQSNIINHTYLAWILMCICPLSKIQAMPQWKEKLESWPGDELPNSGLLMYPVLQAADILLYAPELVPVGEDQIIHLELARYLRNKLINWHRFSPDCELSKYLPLPEMMKCRFPKIYNLKNCKKKMSKSDENGYSRIELMDSPDQIRNKIMKSQTDAFSRNITYDISERPALANLINIYCSVKDSELEGAMFEIEKLSKIQFKELLVDELIQLIDPIRLKIIDLLNNRDEIDRVLKAGHEIANQKANETINIVAHHIGLKS 1211
            + V+SG+QPTG  HLGNY GAI  W++++   +    F  +VDLH+IT       +  + Y +AA  +ACG+  K  ++F QS++  H+ LAW L CI PL+ +Q M Q+KEK  +    E   +GLL+YPVL AADILLY  + VPVGEDQ  HLEL R + N+    H+F+ D  + K LP P +++    ++ +L +  +KMSKSD +  SRI L+D PDQI NKI + +TD   + +T+D  ERP   NL+ +Y  +   + E    E + +   QFK L  +  I  + PI+ K  ++  ++  ++ VL+ G E A   AN+T+  V   +G  +
Sbjct:    4 QRVLSGVQPTGNLHLGNYLGAIRNWVEIQDQYDN---FFCVVDLHAITVPHNPATLAADTYAIAALYLACGIDLKYSNIFVQSHVSAHSELAWFLNCITPLNWLQDMIQFKEK--AVKQGENVGAGLLIYPVLMAADILLYQADKVPVGEDQKQHLELTRDIVNRFN--HQFAKDKPVMK-LPEP-LIRKEGARVMSLTDGTRKMSKSDPSELSRINLLDPPDQIANKIKRCKTDPV-KGLTFDDPERPECNNLLTLYMLLSGKKKEEVAAECQDMGWGQFKPLFTETAINALKPIQEKYQEITADKGYLESVLRDGREKAETVANQTLADVKAALGYSA 333
[Close]
BLAST of Tryptophan--tRNA ligase vs. TrEMBL
Match: T1G8W0 (Uncharacterized protein OS=Helobdella robusta OX=6412 GN=20217507 PE=3 SV=1)

HSP 1 Score: 267.314 bits (682), Expect = 2.369e-82
Identity = 143/338 (42.31%), Postives = 212/338 (62.72%), Query Frame = 3 
Query:  195 TVMSGIQPTGIPHLGNYYGAINQWIKLKSNPNVEQIFISIVDLHSITSYDKLNNVNENVYLMAASLMACGLSGKKVSLFQQSNIINHTYLAWILMCICPLSKIQAMPQWKEKLESWPGDELPNSGLLMYPVLQAADILLYAPELVPVGEDQIIHLELARYLRNKLINWHRFSPDCELSKYLPLPEMMKCRFPKIYNLKNCKKKMSKSDENGYSRIELMDSPDQIRNKIMKSQTDAFSRN-ITYDISERPALANLINIYCSVKDSELEGAMFEIEKLSKIQFKELLVDELIQLIDPIRLKIIDLLNNRDEIDRVLKAGHEIANQKANETINIVAHHIGL 1205
            T+M+GIQPTG PH+GNY GAI Q +KL+++ + ++IF SIVDLHSIT       + +N+  +A SL+ACGL   +  LFQQS++ +HT LAWIL C+  L+K+  +PQWKEK    P  E  + GL  YPVLQAAD+L+Y    VPVG+DQI H+E AR+L  +    +        S   P+P+ +  +  +I +L++  +KMSKS+++   RIEL DSPD+IR K+ K+ TD  +   I YD   RP ++N+I+I+ +V+ S  +    E   L+ +++KE   + +I+++ PIR +I+ L N    +  VL  G+EIA Q A +T   V   IGL
Sbjct:   48 TLMTGIQPTGFPHIGNYLGAIQQSVKLQNSESYDKIFFSIVDLHSITVPQNPTILRQNIINLACSLLACGLDKPQTVLFQQSSVPHHTELAWILGCLATLNKLDHLPQWKEK---SPSKEDVSLGLYSYPVLQAADVLIYRATDVPVGDDQIKHMEFARHLARQFNKIY--------SPVFPIPKQITSQHIRIKSLRDPIRKMSKSEKDSRGRIELFDSPDEIREKLRKAVTDLGTGGMIGYDSQGRPGISNIIDIHAAVEGSTPDQICSECAHLTTVEYKERAAEAIIEMLKPIRARIVHLRNEPQHVVSVLSKGNEIAAQNAAKTCAEVKKLIGL 374
[Close]
BLAST of Tryptophan--tRNA ligase vs. TrEMBL
Match: A0A1D1VUQ2 (Uncharacterized protein OS=Ramazzottius varieornatus OX=947166 GN=RvY_12903-1 PE=3 SV=1)

HSP 1 Score: 259.61 bits (662), Expect = 2.853e-79
Identity = 142/348 (40.80%), Postives = 211/348 (60.63%), Query Frame = 3 
Query:  165 YTNVACKRFKTVMSGIQPTGIPHLGNYYGAINQWIKLKSNPNVEQIFISIVDLHSITSYDKLNNVNENVYLMAASLMACGLSGKKVSLFQQSNIINHTYLAWILMCICPLSKIQAMPQWKEKLESWPGDELPNSGLLMYPVLQAADILLYAPELVPVGEDQIIHLELARYLRNKLINWHRFSPDCELSKYLPLPEMMKCRFPKIYNLKNCKKKMSKSDENGYSRIELMDSPDQIRNKIMKSQTDAFSRNITYDISERPALANLINIYCSVKDSELEGAMFEIEKLSKIQFKELLVDELIQLIDPIRLKIIDLLNNRDEIDRVLKAGHEIANQKANETINIVAHHIGLK 1208
            Y     +   TV SGIQPTG  HLGNY+GA+N W  L+++P    + IS+VD+H+IT   K   + +N+      L+ACG+   KV LFQQS++  H  L WI  C+  ++++  +PQ+KEK  S    E+ + GL  YPVLQAADILLY   LVPVGEDQ+ HL+LA+ +         F+  C   K  P P  +    P++ +L++  KKMSKSD +  S I+L DSP QIR K+ KS TD F   ITYD++ERPA+ANL+ +Y +V D  LE  + E  K++K++ K  L + +I+L+ P+  K+ +L N+   +++V+  G + A   A++T++ V    G +
Sbjct:   26 YNRTQSRTLTTVFSGIQPTGSLHLGNYFGAVNLWKSLQNDP-ANHLIISVVDMHAITVPWKPEQLRQNIVQSFIELLACGIDPAKVVLFQQSSVPEHGKLQWIFSCLSSMARVDNLPQFKEK--SAVQKEV-SCGLFTYPVLQAADILLYKAHLVPVGEDQVQHLQLAQDIARI------FNSKC--GKLFPTPHRLHSDLPRLKSLRDPTKKMSKSDTDSRSLIQLTDSPGQIREKLKKSVTD-FKSEITYDLAERPAIANLLMLYRAVLDVSLEKVLEETSKINKVELKARLAEAVIELLRPVHGKMKELRNDMGYLEQVMAQGTDRARTLASQTMDEVESLAGFR 360
[Close]
BLAST of Tryptophan--tRNA ligase vs. TrEMBL
Match: A0A261Y832 (Uncharacterized protein OS=Bifiguratus adelaidae OX=1938954 GN=BZG36_00280 PE=3 SV=1)

HSP 1 Score: 258.07 bits (658), Expect = 7.001e-79
Identity = 148/357 (41.46%), Postives = 211/357 (59.10%), Query Frame = 3 
Query:  195 TVMSGIQPTGIPHLGNYYGAINQWIKLKSNPNVEQIFISIVDLHSITSYDKLNNVNENVYLMAASLMACGLSGKKVSLFQQSNIINHTYLAWILMCICPLSKIQAMPQWKEKLESWPGDELPNS--------------GLLMYPVLQAADILLYAPELVPVGEDQIIHLELARYLR---NKLINWHRFSPDCELSKYLPLPE-MMKCRFPKIYNLKNCKKKMSKSDENGYSRIELMDSPDQIRNKIMKSQTDAFSRNITYDISERPALANLINIYCSVKDSELEGAMFEIEKLS-KIQFKELLVDELIQLIDPIRLKIIDLLNNRDEIDRVLKAGHEIANQKANETINIVAHHIGLK 1208
            T++SGIQPTG+PHLGNYYGA++ W+KL+       IF SIVDLH++T       + ++   MA +LMACG+  K+  LF+QS +  H  L WIL CI P+  +  M QWK K+ S   D   +S              GL  YPVLQAADIL+Y  + VP+GEDQ+ HLELAR +    NK +    F          PLP+ ++     +I +L+    KMSKSD+N  SRI L D+P+QI+ KI ++ TD+  R+I+YD S RP ++NL+ IY + K+  +E A+     +S   QFKE L D +IQ++ PI+ ++  L N    +D VL+ G   A  +A ET++ V H +GL+
Sbjct:   26 TILSGIQPTGVPHLGNYYGALDNWVKLQRTRRNTTIFYSIVDLHALTIPQDPKVLRQSKRDMAIALMACGVDVKQSVLFEQSRVPQHAELCWILNCITPVGWLSRMTQWKSKISSSNRDAAKHSDAITIAASTEGLRMGLFAYPVLQAADILIYQADEVPIGEDQVQHLELAREIASTFNKTVGEEIF----------PLPKSIIPQSTKRIMSLREPTSKMSKSDDNDMSRINLSDTPEQIKAKIARATTDS-ERSISYDPSSRPGVSNLLAIYAACKNILVEDAVKSFTNVSGHKQFKEDLADAIIQVLGPIQSEMNRLHNESGYVDSVLEKGAVKARNRAAETLDRVYHAVGLR 371
[Close]
BLAST of Tryptophan--tRNA ligase vs. TrEMBL
Match: V4CG83 (Uncharacterized protein OS=Lottia gigantea OX=225164 GN=LOTGIDRAFT_200474 PE=3 SV=1)

HSP 1 Score: 256.144 bits (653), Expect = 1.285e-78
Identity = 152/348 (43.68%), Postives = 206/348 (59.20%), Query Frame = 3 
Query:  177 ACKRFKTVMSGIQPTGIPHLGNYYGAINQWIKLKSNPNVEQIFISIVDLHSITSYDKLNNVNENVYLMAASLMACGLSGKKVSLFQQSNIINHTYLAWILMCICPLSKIQAMPQWKEKLESWPGDELPNSGLLMYPVLQAADILLYAPELVPVGEDQIIHLELARYLRNKLINWHRFSPDCELSKYLPLPEMMKCRFPKIYNLKNCKKKMSKSDENGYSRIELMDSPDQIRNKIMKSQTDAFSRNITYDISERPALANLINIYCSVK----DSELEGAMFEIEKLSKIQFKELLVDELIQLIDPIRLKIIDLLNNRDEIDRVLKAGHEIANQKANETINIVAHHIGLK 1208
            AC R   V SGIQPTGIPH+GNY GAI  W++L+     + + +S+VDLHSIT       + EN+Y M A L+ACG+   K  LFQQS +  HT LAWIL CIC L +++ + QWKEK E    ++   +GLL YP+LQ+ADILLY    VPVG+DQI H+EL+R L          S + +     P P+ +    PKI +LKN K KMSKS+ N   RI+L D PDQIR KI K+ TD+ S  ITYD   RP +ANLI I+ ++     +   E AM  +E +   ++K LL D +I+ + PIR +   L  +R  +D VL+ G   A++ A  T   V   +G +
Sbjct:    8 ACTR--RVFSGIQPTGIPHIGNYVGAIKNWLQLQKK--YDSMILSVVDLHSITLPQNPETLRENIYDMTACLLACGIDSDKCILFQQSTVPQHTQLAWILGCICTLPRLEHLAQWKEKSEK---NKDVGTGLLTYPILQSADILLYKATDVPVGKDQIQHIELSRDLAK--------SFNTKFGVLFPKPQELLGPLPKIKSLKNPKNKMSKSEINPMGRIDLTDEPDQIREKIKKAVTDSTS-EITYDPENRPGVANLIEIHEALTGDFAEDICETAM--LESMDTHKYKLLLTDIVIEKLKPIREEFFRLQKDRHHLDTVLEKGRLNASEIAEVTYKEVTKLVGFR 337
[Close]
BLAST of Tryptophan--tRNA ligase vs. TrEMBL
Match: A0A1C7N9X5 (Tryptophan--tRNA ligase, mitochondrial OS=Choanephora cucurbitarum OX=101091 GN=WARS2 PE=3 SV=1)

HSP 1 Score: 256.529 bits (654), Expect = 2.346e-78
Identity = 151/367 (41.14%), Postives = 218/367 (59.40%), Query Frame = 3 
Query:  168 TNVACKRFKTVMSGIQPTGIPHLGNYYGAINQWIKLK-SNPNVEQI---FISIVDLHSITSYDKLNNVNENVYLMAASLMACGLSGKKVSLFQQSNIINHTYLAWILMCICPLSKIQAMPQWKEKLESWP--------GDELPNSGLLM----YPVLQAADILLYAPELVPVGEDQIIHLELARYLR---NKLINWHRFSPDCELSKYLPLPEMMKCRFPKIYNLKNCKKKMSKSDENGYSRIELMDSPDQIRNKIMKSQTDAFSRNITYDISERPALANLINIYCSVKDSELEGAMFEIEKLSKIQ-FKELLVDELIQLIDPIRLKIIDLLNNRDEIDRVLKAGHEIANQKANETINIVAHHIGLK 1208
            T  A K+   V SGIQPTG PHLGNY GA+  W +L+ ++P   +I   + SIVDLH+IT   K + + +    +AA+L+ACG+  K+  LF+QS + +HT LAW+  CI P+  +  M QWK K+E  P         DE   SGL M    YPVLQAADILLY    VPVGEDQI H+ELAR +    NK   +    P+      + +P   K    ++ +L+    KMSKSD + YSR+ L+DSP  I++KI K+ TD   R +TYD  ERP ++NL++IY +++D E+E A+ E   +S  + FKE + + +I+ I PI+ +++ L  ++  +  VL  G E AN+ A ET+  V   +GL+
Sbjct:   10 TKQAAKKVHRVFSGIQPTGTPHLGNYLGALANWARLQEADPITGEIPLNYYSIVDLHAITMPQKPDTLRQAKLEVAATLLACGVDPKRSVLFEQSRVRSHTELAWVFNCITPVGWLGRMTQWKAKMEKGPSAGHAQSLADETLTSGLKMGLFDYPVLQAADILLYKGTHVPVGEDQIQHIELARDIAGVFNKTFGYTFPKPN------VIVPPAAK----RVMSLREPNSKMSKSDPSDYSRLNLIDSPQLIKSKISKATTDGI-RGVTYDPIERPGVSNLLSIYAAMRDIEIEDAVKECADISSTKDFKERVAEAIIERIQPIQSELVRLQKDQGYVRNVLDEGAEKANEVAEETMEQVYKAVGLR 365
[Close]
BLAST of Tryptophan--tRNA ligase vs. Ensembl Cavefish
Match: wars2 (tryptophanyl tRNA synthetase 2, mitochondrial [Source:ZFIN;Acc:ZDB-GENE-050306-26])

HSP 1 Score: 224.942 bits (572), Expect = 1.191e-69
Identity = 132/353 (37.39%), Postives = 197/353 (55.81%), Query Frame = 3 
Query:  147 FYFSKLYTNVACKRFKTVMSGIQPTGIPHLGNYYGAINQWIKLKSNPNVEQIFISIVDLHSITSYDKLNNVNENVYLMAASLMACGLSGKKVSLFQQSNIINHTYLAWILMCICPLSKIQAMPQWKEKLESWPGDELPNSGLLMYPVLQAADILLYAPELVPVGEDQIIHLELARYLRNKLINWHRFSPDCELSKYLPLPEMMKCRFPKIYNLKNCKKKMSKSDENGYSRIELMDSPDQIRNKIMKSQTDAFSRNITYDISERPALANLINIYCSVKDSELEGAMFEIEKLSKIQFKELLVDELIQLIDPIRLKIIDLLNNRDEIDRVLKAGHEIANQKANETINIVAHHIGL 1205
            F F  L T+ +  R   V SGIQPTG+PHLGNY GA+  W+ L++      +  SIVDLHSIT       + E++  MAASL+ACG+   +  LFQQS +  H  L WIL C+  + +++ +PQW  K++S   +E  + GL  YPVLQAADILLY    VPVGEDQ+ HLELA+ L     N +         +  P P  +     K+ +L++   KMSKSD   ++ I L D+P+ I  KI ++ TD F+  +T+D   RP ++NL++++ +V    +E  + +   L   ++K L+ + ++Q + PIRL+I  L  +R  +  VL  G   A   A   +  V   +G 
Sbjct:   13 FRFIVLITDPSLMR---VFSGIQPTGVPHLGNYLGALESWVSLQNE--YSSVLYSIVDLHSITQPQDPQLLRESILDMAASLLACGIDPARSILFQQSVVSEHAELCWILGCLTSMPRLRHLPQW--KMKSKQKNE-GSVGLYTYPVLQAADILLYKATHVPVGEDQVQHLELAQDLARIFNNKY--------GELFPEPRALLSSTRKVKSLRDPSCKMSKSDPQKFATIFLTDTPEDIVFKIRRAVTD-FTSEVTFDPEGRPGVSNLVSLHAAVSGLTVEDVLIQARGLDTGKYKSLVSEAVVQRLQPIRLEIQRLRADRGHLLSVLAEGGAKARSLAAPVMTEVRKRVGF 348
[Close]
BLAST of Tryptophan--tRNA ligase vs. Ensembl Cavefish
Match: wars2 (tryptophanyl tRNA synthetase 2, mitochondrial [Source:ZFIN;Acc:ZDB-GENE-050306-26])

HSP 1 Score: 223.016 bits (567), Expect = 1.290e-68
Identity = 126/336 (37.50%), Postives = 187/336 (55.65%), Query Frame = 3 
Query:  198 VMSGIQPTGIPHLGNYYGAINQWIKLKSNPNVEQIFISIVDLHSITSYDKLNNVNENVYLMAASLMACGLSGKKVSLFQQSNIINHTYLAWILMCICPLSKIQAMPQWKEKLESWPGDELPNSGLLMYPVLQAADILLYAPELVPVGEDQIIHLELARYLRNKLINWHRFSPDCELSKYLPLPEMMKCRFPKIYNLKNCKKKMSKSDENGYSRIELMDSPDQIRNKIMKSQTDAFSRNITYDISERPALANLINIYCSVKDSELEGAMFEIEKLSKIQFKELLVDELIQLIDPIRLKIIDLLNNRDEIDRVLKAGHEIANQKANETINIVAHHIGL 1205
            V SGIQPTG+PHLGNY GA+  W+ L++      +  SIVDLHSIT       + E++  MAASL+ACG+   +  LFQQS +  H  L WIL C+  + +++ +PQWK K +        + GL  YPVLQAADILLY    VPVGEDQ+ HLELA+ L     N        +  +  P P  +     K+ +L++   KMSKSD   ++ I L D+P+ I  KI ++ TD F+  +T+D   RP ++NL++++ +V    +E  + +   L   ++K L+ + ++Q + PIRL+I  L  +R  +  VL  G   A   A   +  V   +G 
Sbjct:   53 VFSGIQPTGVPHLGNYLGALESWVSLQNE--YSSVLYSIVDLHSITQPQDPQLLRESILDMAASLLACGIDPARSILFQQSVVSEHAELCWILGCLTSMPRLRHLPQWKMKSKQ---KNEGSVGLYTYPVLQAADILLYKATHVPVGEDQVQHLELAQDLARIFNN--------KYGELFPEPRALLSSTRKVKSLRDPSCKMSKSDPQKFATIFLTDTPEDIVFKIRRAVTD-FTSEVTFDPEGRPGVSNLVSLHAAVSGLTVEDVLIQARGLDTGKYKSLVSEAVVQRLQPIRLEIQRLRADRGHLLSVLAEGGAKARSLAAPVMTEVRKRVGF 374
[Close]
BLAST of Tryptophan--tRNA ligase vs. Ensembl Yeast
Match: MSW1 (Mitochondrial tryptophanyl-tRNA synthetase [Source:SGD;Acc:S000002676])

HSP 1 Score: 181.415 bits (459), Expect = 1.396e-53
Identity = 123/352 (34.94%), Postives = 178/352 (50.57%), Query Frame = 3 
Query:  195 TVMSGIQPTGIPHLGNYYGAINQWIKL--KSNPNVEQIFISIVDLHSITSYDKLNNVNENV-YLMAASLMACGLSGKKVSLFQQSNIINHTYLAWILMCICPLSKIQAMPQWKEKL---ESWPGDELPNS--------GLLMYPVLQAADILLYAPELVPVGEDQIIHLELARYLRNKLINWHRFSPDCELSKYLPLPEMMKCRFPKIYNLKNCKKKMSKSDENGYSRIELMDSPDQIRNKIMKSQTDAFSRNITYDISERPALANLINIYCSVKDSELEGAMFEIEKLSKIQ-FKELLVDELIQLIDPIRLKIIDLLNNRDEIDRVLKAGHEIANQKANETINIVAHHIGL 1205
            TV S IQPTG  HLGNY GA   W  L     P  E IF  + DLH+IT       +     +   AS++A G+  +K S+  QS I  H+ L W+L  +  +  +  M QWK K    +S  GD L N         GL  YPVLQAADILLY    VPVG+DQ  HLEL R+L  K    ++         + P P  M  +  K+ +L   +KKMSKSD N  S I L D P  I+ KI K+ TD+ S    YD  ERP ++NLINI   ++   +E  + ++ + +  + FK+ + + +I+ +   R +    +N    +  V+++G   A +KA + +  +   +G 
Sbjct:   36 TVFSMIQPTGCFHLGNYLGATRVWTDLCELKQPGQELIF-GVADLHAITVPKPDGEMFRKFRHEAVASILAVGVDPEKASVIYQSAIPQHSELHWLLSTLASMGLLNRMTQWKSKSNIKQSTNGDYLVNDSDVGKVRLGLFSYPVLQAADILLYKSTHVPVGDDQSQHLELTRHLAEKFNKMYK-------KNFFPKPVTMLAQTKKVLSLSTPEKKMSKSDPNHDSVIFLNDEPKAIQKKIRKALTDSISDRFYYDPVERPGVSNLINIVSGIQRKSIEDVVEDVSRFNNYRDFKDYVSEVIIEELKGPRTEFEKYINEPTYLHSVVESGMRKAREKAAKNLADIHKIMGF 379
[Close]
BLAST of Tryptophan--tRNA ligase vs. Ensembl Nematostella
Match: EDO41116 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S5F6])

HSP 1 Score: 214.157 bits (544), Expect = 8.566e-66
Identity = 131/344 (38.08%), Postives = 185/344 (53.78%), Query Frame = 3 
Query:  183 KRFKTVMSGIQPTGIPHLGNYYGAINQWIKLKSNPNVEQIFISIVDLHSITSYDKLNNVNENVYLMAASLMACGLSGKKVSLFQQSNIINHTYLAWILMCICPLSKIQAMPQWKEKLESWPGDELPNSGLLMYPVLQAADILLYAPELVPVGEDQIIHLELARYLRNKLINWHRFSPDCELSKYLPLPEMMKCRFPKIYNLKNCKKKMSKSDENGYSRIELMDSPDQIRNKIMKSQTDAFSRNITYDISERPALANLINIYCSVKDSELEG--AMFEIEKLSKIQFKELLVDELIQLIDPIRLKIIDLLNNRDEIDRVLKAGHEIANQKANETINIVAHHIGLK 1208
            ++ K + SGIQPTG  HLGN+ GAI  W+ ++       I+ SI DLHSIT   + + + ++V  MA SL+A G++  +  LFQQS +  H  LAW+L C+ P   +  M QWK K            GL  YPVL AADILLY    VP+GEDQ+ HLEL R L  ++ N            +   P  +     ++ +L++  KKMSKSD    SRIEL D+ DQI  KI ++ TD+    I YD   RP L+NLI +Y ++ +   E     ++  + SK +FK+ L D +I  + PIR  I  L  +   +  VL+ G E A   A ET+  V   +GL+
Sbjct:   34 RKPKCIFSGIQPTGAMHLGNFLGAIQSWVSMQHESEARVIY-SITDLHSITVPQEPSVLKQSVLNMATSLLASGVNPDRCILFQQSQVSQHAELAWVLSCLAPTGNLSRMHQWKSKTNQTKARTC--LGLYAYPVLMAADILLYRATHVPIGEDQLQHLELTREL-ARIYNQ-------TYGNFFMQPHAILGSTQRVMSLRDPTKKMSKSDPVPSSRIELTDTADQIAAKIRRAVTDS-EPYICYDPGTRPGLSNLIRVYAALSNESPEAISVRYQDTEHSKARFKQQLTDLVIHTLQPIRENIQSLSKDPGLVSNVLETGAEKARLLAEETLVQVRRAVGLR 365
[Close]
BLAST of Tryptophan--tRNA ligase vs. Ensembl Nematostella
Match: EDO28469 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T6D6])

HSP 1 Score: 74.7146 bits (182), Expect = 7.201e-17
Identity = 38/87 (43.68%), Postives = 55/87 (63.22%), Query Frame = 3 
Query:  192 KTVMSGIQPTGIPHLGNYYGAINQWIKLKSNPNVEQIFISIVDLHSITSYDKLNNVNENVYLMAASLMACGLSGKKVSLFQQSNIIN 452
            K + SGIQPTG  HLGN+ GAI  W+ ++       I+ SI DLHSIT   + + + ++V  MA SL+A G++  +  LFQQS ++N
Sbjct:    1 KCIFSGIQPTGAMHLGNFLGAIQSWVSMQHESEARVIY-SITDLHSITVPQEPSVLKQSVLNMATSLLASGVNPDRCILFQQSQVMN 86
[Close]
BLAST of Tryptophan--tRNA ligase vs. Ensembl Medaka
Match: wars2 (tryptophanyl tRNA synthetase 2, mitochondrial [Source:NCBI gene;Acc:101173101])

HSP 1 Score: 230.72 bits (587), Expect = 1.035e-71
Identity = 128/336 (38.10%), Postives = 195/336 (58.04%), Query Frame = 3 
Query:  198 VMSGIQPTGIPHLGNYYGAINQWIKLKSNPNVEQIFISIVDLHSITSYDKLNNVNENVYLMAASLMACGLSGKKVSLFQQSNIINHTYLAWILMCICPLSKIQAMPQWKEKLESWPGDELPNSGLLMYPVLQAADILLYAPELVPVGEDQIIHLELARYLRNKLINWHRFSPDCELSKYLPLPEMMKCRFPKIYNLKNCKKKMSKSDENGYSRIELMDSPDQIRNKIMKSQTDAFSRNITYDISERPALANLINIYCSVKDSELEGAMFEIEKLSKIQFKELLVDELIQLIDPIRLKIIDLLNNRDEIDRVLKAGHEIANQKANETINIVAHHIGL 1205
            V SGIQPTG+PHLGN+ GA+  W+ L++      +  SIVDLHSIT     + +  N+  + ASL+ACG+  +K  LFQQS +  H  LAWIL C+  + +++ +PQW  K++S   +E  + GL  YPVLQAADILLY    VPVGEDQ+ HLELA+ L  ++ N H            P P  +     K+ +L++   KMSKSD    + I + DSP+ I  KI ++ TD F+  +T+D   RP ++NL+ I+ ++    +E A+ +   L    +K+L+ D ++Q + PIR +I  L ++R  ++ +L  G + A + A   +  V H +G 
Sbjct:   41 VFSGIQPTGVPHLGNFLGALENWVALQNQ--YPTVLYSIVDLHSITQPQDPDQLRSNILDLTASLLACGIDPEKAILFQQSQVSEHAELAWILGCMTSMPRLRHLPQW--KMKSGQKNE-GSVGLFTYPVLQAADILLYQSTHVPVGEDQVQHLELAQDL-ARIFNNH-------YGDLFPEPRTLLSSTRKVKSLRDPSAKMSKSDPQTMATITITDSPEDIALKIRRAVTD-FTSEVTFDPETRPGVSNLVMIHAAMAAISVEEALSQARGLDTGAYKKLVSDAVVQRLTPIRHEIGKLRSDRAYLEGLLAQGRKKARELAAPVLAEVRHRVGF 362
[Close]
BLAST of Tryptophan--tRNA ligase vs. Ensembl Medaka
Match: wars2 (tryptophanyl tRNA synthetase 2, mitochondrial [Source:NCBI gene;Acc:101173101])

HSP 1 Score: 228.024 bits (580), Expect = 5.851e-71
Identity = 129/337 (38.28%), Postives = 195/337 (57.86%), Query Frame = 3 
Query:  198 VMSGIQPTGIPHLGNYYGAINQWIKLKSNPNVEQIFISIVDLHSITSYDKLNNVNENVYLMAASLMACGLSGKKVSLFQQSNIINHTYLAWILMCICPLSKIQAMPQWKEKLESWPGDELPNS-GLLMYPVLQAADILLYAPELVPVGEDQIIHLELARYLRNKLINWHRFSPDCELSKYLPLPEMMKCRFPKIYNLKNCKKKMSKSDENGYSRIELMDSPDQIRNKIMKSQTDAFSRNITYDISERPALANLINIYCSVKDSELEGAMFEIEKLSKIQFKELLVDELIQLIDPIRLKIIDLLNNRDEIDRVLKAGHEIANQKANETINIVAHHIGL 1205
            V SGIQPTG+PHLGN+ GA+  W+ L++      +  SIVDLHSIT     + +  N+  + ASL+ACG+  +K  LFQQS +  H  LAWIL C+  + +++ +PQWK K     G +   S GL  YPVLQAADILLY    VPVGEDQ+ HLELA+ L  ++ N H      +L    P   ++     K+ +L++   KMSKSD    + I + DSP+ I  KI ++ TD F+  +T+D   RP ++NL+ I+ ++    +E A+ +   L    +K+L+ D ++Q + PIR +I  L ++R  ++ +L  G + A + A   +  V H +G 
Sbjct:   23 VFSGIQPTGVPHLGNFLGALENWVALQNQ--YPTVLYSIVDLHSITQPQDPDQLRSNILDLTASLLACGIDPEKAILFQQSQVSEHAELAWILGCMTSMPRLRHLPQWKMK----SGQKNEGSVGLFTYPVLQAADILLYQSTHVPVGEDQVQHLELAQDL-ARIFNNHY----GDLFPEPPFSFILTGSTRKVKSLRDPSAKMSKSDPQTMATITITDSPEDIALKIRRAVTD-FTSEVTFDPETRPGVSNLVMIHAAMAAISVEEALSQARGLDTGAYKKLVSDAVVQRLTPIRHEIGKLRSDRAYLEGLLAQGRKKARELAAPVLAEVRHRVGF 347
[Close]
BLAST of Tryptophan--tRNA ligase vs. Ensembl Medaka
Match: wars2 (tryptophanyl tRNA synthetase 2, mitochondrial [Source:NCBI gene;Acc:101173101])

HSP 1 Score: 191.815 bits (486), Expect = 4.487e-57
Identity = 117/336 (34.82%), Postives = 177/336 (52.68%), Query Frame = 3 
Query:  198 VMSGIQPTGIPHLGNYYGAINQWIKLKSNPNVEQIFISIVDLHSITSYDKLNNVNENVYLMAASLMACGLSGKKVSLFQQSNIINHTYLAWILMCICPLSKIQAMPQWKEKLESWPGDELPNSGLLMYPVLQAADILLYAPELVPVGEDQIIHLELARYLRNKLINWHRFSPDCELSKYLPLPEMMKCRFPKIYNLKNCKKKMSKSDENGYSRIELMDSPDQIRNKIMKSQTDAFSRNITYDISERPALANLINIYCSVKDSELEGAMFEIEKLSKIQFKELLVDELIQLIDPIRLKIIDLLNNRDEIDRVLKAGHEIANQKANETINIVAHHIGL 1205
            V SGIQPTG+PHLGN+ GA+  W+ L++      +  SIVDLHSIT     + +  N+  + ASL+ACG+  +K  LFQQS +                       +  +K E        + GL  YPVLQAADILLY    VPVGEDQ+ HLELA+ L  ++ N H            P P  +     K+ +L++   KMSKSD    + I + DSP+ I  KI ++ TD F+  +T+D   RP ++NL+ I+ ++    +E A+ +   L    +K+L+ D ++Q + PIR +I  L ++R  ++ +L  G + A + A   +  V H +G 
Sbjct:   41 VFSGIQPTGVPHLGNFLGALENWVALQNQ--YPTVLYSIVDLHSITQPQDPDQLRSNILDLTASLLACGIDPEKAILFQQSQM-----------------------KSGQKNEG-------SVGLFTYPVLQAADILLYQSTHVPVGEDQVQHLELAQDL-ARIFNNH-------YGDLFPEPRTLLSSTRKVKSLRDPSAKMSKSDPQTMATITITDSPEDIALKIRRAVTD-FTSEVTFDPETRPGVSNLVMIHAAMAAISVEEALSQARGLDTGAYKKLVSDAVVQRLTPIRHEIGKLRSDRAYLEGLLAQGRKKARELAAPVLAEVRHRVGF 335
[Close]
BLAST of Tryptophan--tRNA ligase vs. Planmine SMEST
Match: SMESG000019268.1 (SMESG000019268.1)

HSP 1 Score: 758.829 bits (1958), Expect = 0.000e+0
Identity = 367/373 (98.39%), Postives = 371/373 (99.46%), Query Frame = 3 
Query:  135 MSRIFYFSKLYTNVACKRFKTVMSGIQPTGIPHLGNYYGAINQWIKLKSNPNVEQIFISIVDLHSITSYDKLNNVNENVYLMAASLMACGLSGKKVSLFQQSNIINHTYLAWILMCICPLSKIQAMPQWKEKLESWPGDELPNSGLLMYPVLQAADILLYAPELVPVGEDQIIHLELARYLRNKLINWHRFSPDCELSKYLPLPEMMKCRFPKIYNLKNCKKKMSKSDENGYSRIELMDSPDQIRNKIMKSQTDAFSRNITYDISERPALANLINIYCSVKDSELEGAMFEIEKLSKIQFKELLVDELIQLIDPIRLKIIDLLNNRDEIDRVLKAGHEIANQKANETINIVAHHIGLKSHALDSYPVKSKCNQ 1253
            MSRIFYFSKLYTNVACKRFKTVMSGIQPTGIPHLGNYYGAINQWIKLKSNPNVEQIFISIVDLHSITSYDKLNN+NENVYLMAASLMACGLSGKKVSLFQQSNIINHTYLAWILMCICPLS+IQAMPQWKEKLESWPGDELPNSGLLMYPVLQAADILLYAP+LVPVGEDQIIHLELARYLRNKLINWHRFSPDCELSKYLPLPEMMKCRFPKIYNLKNCKKKMSKSDEN YSRIELMDSPDQIRNKIMKSQTDAFSRNITYDI+ERPALANLINIYCSVKDSELE AMFEIEKLSKIQFKELLVDELIQLIDPIRLKIIDLLNNRDEIDRVLKAGHEIANQKANETINIVAHHIGLKSHALDSYPVKSKCNQ
Sbjct:    1 MSRIFYFSKLYTNVACKRFKTVMSGIQPTGIPHLGNYYGAINQWIKLKSNPNVEQIFISIVDLHSITSYDKLNNLNENVYLMAASLMACGLSGKKVSLFQQSNIINHTYLAWILMCICPLSRIQAMPQWKEKLESWPGDELPNSGLLMYPVLQAADILLYAPQLVPVGEDQIIHLELARYLRNKLINWHRFSPDCELSKYLPLPEMMKCRFPKIYNLKNCKKKMSKSDENSYSRIELMDSPDQIRNKIMKSQTDAFSRNITYDINERPALANLINIYCSVKDSELEDAMFEIEKLSKIQFKELLVDELIQLIDPIRLKIIDLLNNRDEIDRVLKAGHEIANQKANETINIVAHHIGLKSHALDSYPVKSKCNQ 373
[Close]
BLAST of Tryptophan--tRNA ligase vs. Planmine SMEST
Match: SMESG000019268.1 (SMESG000019268.1)

HSP 1 Score: 709.909 bits (1831), Expect = 0.000e+0
Identity = 345/351 (98.29%), Postives = 349/351 (99.43%), Query Frame = 3 
Query:  201 MSGIQPTGIPHLGNYYGAINQWIKLKSNPNVEQIFISIVDLHSITSYDKLNNVNENVYLMAASLMACGLSGKKVSLFQQSNIINHTYLAWILMCICPLSKIQAMPQWKEKLESWPGDELPNSGLLMYPVLQAADILLYAPELVPVGEDQIIHLELARYLRNKLINWHRFSPDCELSKYLPLPEMMKCRFPKIYNLKNCKKKMSKSDENGYSRIELMDSPDQIRNKIMKSQTDAFSRNITYDISERPALANLINIYCSVKDSELEGAMFEIEKLSKIQFKELLVDELIQLIDPIRLKIIDLLNNRDEIDRVLKAGHEIANQKANETINIVAHHIGLKSHALDSYPVKSKCNQ 1253
            MSGIQPTGIPHLGNYYGAINQWIKLKSNPNVEQIFISIVDLHSITSYDKLNN+NENVYLMAASLMACGLSGKKVSLFQQSNIINHTYLAWILMCICPLS+IQAMPQWKEKLESWPGDELPNSGLLMYPVLQAADILLYAP+LVPVGEDQIIHLELARYLRNKLINWHRFSPDCELSKYLPLPEMMKCRFPKIYNLKNCKKKMSKSDEN YSRIELMDSPDQIRNKIMKSQTDAFSRNITYDI+ERPALANLINIYCSVKDSELE AMFEIEKLSKIQFKELLVDELIQLIDPIRLKIIDLLNNRDEIDRVLKAGHEIANQKANETINIVAHHIGLKSHALDSYPVKSKCNQ
Sbjct:    1 MSGIQPTGIPHLGNYYGAINQWIKLKSNPNVEQIFISIVDLHSITSYDKLNNLNENVYLMAASLMACGLSGKKVSLFQQSNIINHTYLAWILMCICPLSRIQAMPQWKEKLESWPGDELPNSGLLMYPVLQAADILLYAPQLVPVGEDQIIHLELARYLRNKLINWHRFSPDCELSKYLPLPEMMKCRFPKIYNLKNCKKKMSKSDENSYSRIELMDSPDQIRNKIMKSQTDAFSRNITYDINERPALANLINIYCSVKDSELEDAMFEIEKLSKIQFKELLVDELIQLIDPIRLKIIDLLNNRDEIDRVLKAGHEIANQKANETINIVAHHIGLKSHALDSYPVKSKCNQ 351
The following BLAST results are available for this feature:
BLAST of Tryptophan--tRNA ligase vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 2
Match NameE-valueIdentityDescription
WARS26.177e-6836.39tryptophanyl tRNA synthetase 2, mitochondrial [Sou... [more]
WARS21.088e-4345.16tryptophanyl tRNA synthetase 2, mitochondrial [Sou... [more]
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BLAST of Tryptophan--tRNA ligase vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 1
Match NameE-valueIdentityDescription
prx-104.373e-5635.10Putative peroxisomal biogenesis factor 10 [Source... [more]
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BLAST of Tryptophan--tRNA ligase vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 2
Match NameE-valueIdentityDescription
TrpRS-m1.979e-6436.52gene:FBgn0036763 transcript:FBtr0075141[more]
TrpRS-m1.979e-6436.52gene:FBgn0036763 transcript:FBtr0331811[more]
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BLAST of Tryptophan--tRNA ligase vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
wars21.761e-7237.75tryptophanyl tRNA synthetase 2, mitochondrial [Sou... [more]
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BLAST of Tryptophan--tRNA ligase vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 2
Match NameE-valueIdentityDescription
WARS21.202e-4936.10tryptophanyl tRNA synthetase 2, mitochondrial [Sou... [more]
WARS28.804e-4133.33tryptophanyl tRNA synthetase 2, mitochondrial [Sou... [more]
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BLAST of Tryptophan--tRNA ligase vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 4
Match NameE-valueIdentityDescription
Wars23.482e-6736.90tryptophanyl tRNA synthetase 2 (mitochondrial) [So... [more]
Wars23.585e-3448.92tryptophanyl tRNA synthetase 2 (mitochondrial) [So... [more]
Wars24.005e-3448.25tryptophanyl tRNA synthetase 2 (mitochondrial) [So... [more]
Wars22.875e-951.85tryptophanyl tRNA synthetase 2 (mitochondrial) [So... [more]
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BLAST of Tryptophan--tRNA ligase vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q3T099|SYWM_BOVIN7.325e-6936.76Tryptophan--tRNA ligase, mitochondrial OS=Bos taur... [more]
sp|Q7VBM9|SYW_PROMA1.005e-6840.53Tryptophan--tRNA ligase OS=Prochlorococcus marinus... [more]
sp|Q92HR1|SYW_RICCN2.888e-6839.82Tryptophan--tRNA ligase OS=Rickettsia conorii (str... [more]
sp|Q9UGM6|SYWM_HUMAN2.966e-6736.39Tryptophan--tRNA ligase, mitochondrial OS=Homo sap... [more]
sp|Q8YXE4|SYW_NOSS13.489e-6738.82Tryptophan--tRNA ligase OS=Nostoc sp. (strain PCC ... [more]
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BLAST of Tryptophan--tRNA ligase vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
T1G8W02.369e-8242.31Uncharacterized protein OS=Helobdella robusta OX=6... [more]
A0A1D1VUQ22.853e-7940.80Uncharacterized protein OS=Ramazzottius varieornat... [more]
A0A261Y8327.001e-7941.46Uncharacterized protein OS=Bifiguratus adelaidae O... [more]
V4CG831.285e-7843.68Uncharacterized protein OS=Lottia gigantea OX=2251... [more]
A0A1C7N9X52.346e-7841.14Tryptophan--tRNA ligase, mitochondrial OS=Choaneph... [more]
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BLAST of Tryptophan--tRNA ligase vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
wars21.191e-6937.39tryptophanyl tRNA synthetase 2, mitochondrial [Sou... [more]
wars21.290e-6837.50tryptophanyl tRNA synthetase 2, mitochondrial [Sou... [more]
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BLAST of Tryptophan--tRNA ligase vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Tryptophan--tRNA ligase vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 1
Match NameE-valueIdentityDescription
MSW11.396e-5334.94Mitochondrial tryptophanyl-tRNA synthetase [Source... [more]
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BLAST of Tryptophan--tRNA ligase vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 2
Match NameE-valueIdentityDescription
EDO411168.566e-6638.08Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO284697.201e-1743.68Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Tryptophan--tRNA ligase vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 3
Match NameE-valueIdentityDescription
wars21.035e-7138.10tryptophanyl tRNA synthetase 2, mitochondrial [Sou... [more]
wars25.851e-7138.28tryptophanyl tRNA synthetase 2, mitochondrial [Sou... [more]
wars24.487e-5734.82tryptophanyl tRNA synthetase 2, mitochondrial [Sou... [more]
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BLAST of Tryptophan--tRNA ligase vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000019268.10.000e+098.39SMESG000019268.1[more]
SMESG000019268.10.000e+098.29SMESG000019268.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30023152 ID=SMED30023152|Name=Tryptophan--tRNA ligase|organism=Schmidtea mediterranea sexual|type=transcript|length=1278bp
GATATTAATCACTTGTAATTTATGTTATTAATATATGTATTTTAAATTTA
GTCTAAATTCGCATTATTTCAAATTGTTTGTAGAATAGAATTTCCAAATA
ATAATTCTAACAGTTGTAAAACATTAATTCTTTAATGAGTCGAATATTTT
ATTTTTCTAAATTATATACCAATGTTGCTTGCAAAAGATTCAAAACTGTT
ATGTCTGGAATTCAGCCTACAGGAATTCCTCATTTAGGGAATTATTATGG
TGCAATAAATCAGTGGATAAAATTGAAAAGTAATCCAAATGTTGAACAAA
TCTTTATTAGTATTGTTGACTTGCATTCAATTACTTCATATGATAAACTT
AATAATGTGAACGAAAATGTTTATCTCATGGCAGCCTCTTTAATGGCTTG
TGGTTTGAGTGGGAAAAAAGTTTCATTATTTCAGCAATCTAATATAATAA
ATCATACGTATCTTGCATGGATATTGATGTGTATTTGTCCATTAAGTAAA
ATTCAAGCCATGCCTCAATGGAAAGAAAAATTAGAATCATGGCCTGGCGA
TGAACTGCCGAATTCTGGATTGTTAATGTATCCGGTTCTTCAAGCAGCCG
ACATTTTATTATATGCTCCGGAATTAGTACCAGTTGGAGAAGATCAAATT
ATTCACCTGGAATTAGCCCGATATCTTCGAAATAAATTGATAAATTGGCA
TCGATTCAGTCCGGATTGCGAATTATCCAAATATTTGCCACTCCCTGAAA
TGATGAAATGTCGATTTCCAAAGATTTATAATTTGAAAAATTGCAAGAAA
AAAATGTCGAAATCCGATGAGAACGGTTATTCGAGAATTGAATTGATGGA
TTCTCCTGATCAAATCAGAAACAAGATTATGAAATCTCAAACTGATGCGT
TTTCAAGAAATATAACTTACGATATAAGTGAACGTCCGGCATTGGCTAAT
CTGATCAACATCTATTGTTCGGTTAAGGATTCAGAACTAGAAGGTGCAAT
GTTTGAGATTGAAAAGCTTTCAAAGATTCAATTCAAAGAATTATTAGTTG
ATGAACTAATACAACTAATTGATCCGATCAGATTGAAAATTATAGATTTA
TTGAATAATCGTGACGAAATTGATCGAGTGTTAAAGGCTGGGCATGAAAT
CGCCAATCAAAAGGCGAATGAAACAATCAATATCGTTGCACATCACATTG
GTTTAAAAAGCCATGCGCTTGATAGTTATCCGGTGAAATCAAAATGTAAT
CAATAAATATTTCTGTTTCTCAAAAAGA
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protein sequence of SMED30023152-orf-1

>SMED30023152-orf-1 ID=SMED30023152-orf-1|Name=SMED30023152-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=374bp
MSRIFYFSKLYTNVACKRFKTVMSGIQPTGIPHLGNYYGAINQWIKLKSN
PNVEQIFISIVDLHSITSYDKLNNVNENVYLMAASLMACGLSGKKVSLFQ
QSNIINHTYLAWILMCICPLSKIQAMPQWKEKLESWPGDELPNSGLLMYP
VLQAADILLYAPELVPVGEDQIIHLELARYLRNKLINWHRFSPDCELSKY
LPLPEMMKCRFPKIYNLKNCKKKMSKSDENGYSRIELMDSPDQIRNKIMK
SQTDAFSRNITYDISERPALANLINIYCSVKDSELEGAMFEIEKLSKIQF
KELLVDELIQLIDPIRLKIIDLLNNRDEIDRVLKAGHEIANQKANETINI
VAHHIGLKSHALDSYPVKSKCNQ*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0002142parenchyma
Vocabulary: INTERPRO
TermDefinition
IPR002306Trp-tRNA-ligase
IPR014729Rossmann-like_a/b/a_fold
IPR002305aa-tRNA-synth_Ic
IPR001412aa-tRNA-synth_I_CS
Vocabulary: biological process
TermDefinition
GO:0006436tryptophanyl-tRNA aminoacylation
GO:0006418tRNA aminoacylation for protein translation
GO:0006412translation
Vocabulary: molecular function
TermDefinition
GO:0005524ATP binding
GO:0004830tryptophan-tRNA ligase activity
GO:0000166nucleotide binding
GO:0004812aminoacyl-tRNA ligase activity
GO:0016874ligase activity
Vocabulary: cellular component
TermDefinition
GO:0005737cytoplasm
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002306Tryptophan-tRNA ligasePRINTSPR01039TRNASYNTHTRPcoord: 32..48
score: 35.9
coord: 163..184
score: 45.77
coord: 223..233
score: 55.17
coord: 85..104
score: 32.76
IPR002306Tryptophan-tRNA ligaseTIGRFAMTIGR00233TIGR00233coord: 20..357
e-value: 1.3E-72
score: 242.8
NoneNo IPR availableGENE3DG3DSA:1.10.240.10coord: 214..325
e-value: 4.7E-104
score: 350.1
NoneNo IPR availablePANTHERPTHR43766:SF1TRYPTOPHAN--TRNA LIGASE, MITOCHONDRIALcoord: 17..357
NoneNo IPR availablePANTHERPTHR43766FAMILY NOT NAMEDcoord: 17..357
NoneNo IPR availableSUPERFAMILYSSF52374Nucleotidylyl transferasecoord: 21..357
IPR002305Aminoacyl-tRNA synthetase, class IcPFAMPF00579tRNA-synt_1bcoord: 21..288
e-value: 2.6E-55
score: 187.8
IPR014729Rossmann-like alpha/beta/alpha sandwich foldGENE3DG3DSA:3.40.50.620coord: 22..356
e-value: 4.7E-104
score: 350.1
IPR001412Aminoacyl-tRNA synthetase, class I, conserved sitePROSITEPS00178AA_TRNA_LIGASE_Icoord: 28..37