SMED30023147

Overview
NameSMED30023147
Smed IDSMED30023147
Length (bp)1512
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30023147 (SMED30023147) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30023147 (SMED30023147) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30023147 (SMED30023147) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30023147 (SMED30023147) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30023147

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 8

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30023147SMESG000032765.1 SMESG000032764.1 SmedASXL_010686SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015
FACS sorted cell population asexual adult RNA-sequencing evidence
nervous systemSMED30023147SMESG000032765.1 SMESG000032764.1 dd_Smed_v4_9225_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30023147SMESG000032765.1 SMESG000032764.1 dd_Smed_v4_9225_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30023147SMESG000032765.1 SMESG000032764.1 dd_Smed_v4_9225_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymaSMED30023147SMESG000032765.1 SMESG000032764.1 dd_Smed_v4_9225_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
zeta neoblastSMED30023147SMESG000032765.1 SMESG000032764.1 dd_Smed_v4_9225_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017
whole organism asexual adult single-cell RNA-sequencing evidence
neoblastSMED30023147SMESG000032765.1 SMED_09519_V2_1GPL14150_gene_modelsPMID:22385657
Wagner et al., 2012
whole organism asexual adult cDNA to DNA expression microarray evidence
neoblastSMED30023147SMESG000032765.1 SMED_09519_V2_1GPL14150PMID:22385657
Wagner et al., 2012
whole organism asexual adult cDNA to DNA expression microarray evidence
Note: Hover over icons to view figure legend
Homology
BLAST of SMED30023147 vs. Planmine SMEST
Match: SMESG000032769.1 (SMESG000032769.1)

HSP 1 Score: 502.671 bits (1293), Expect = 2.755e-177
Identity = 252/340 (74.12%), Postives = 284/340 (83.53%), Query Frame = 3
Query:  360 NRNNKNEFVNEIECQNATAYSRIMIGRSRNQSKRIERKKNVNKLPSSASRNVSDGKANVTSAKLTKIFVGKHEVYASGCGGALEVHKYNKATTTIPSGTTDDILGFYLISEKLLLYTASELILTTKRYGRNMLHCADPPEVLRDFKPDTIINVLRLNKLFQVTLENGESWDYNFYEKTFTNVFSWKLRLKSIITKLNQSVALTSDHRVYRSIDNGNPVEIKYLEGCQHISLSFNKQFVAIFPQTICWVNWKSCNIVKQTRIKVPIKSV-FYSPLLLTVDVKNAIVTFQ--QGGQIRYPTGALVKEFPHNKEVTALSNVFFKKIYVAIDNEVHLLSRQPLQ 1370
            N ++ NEFVNEIE QNAT YSRIMIGRSRNQSKRIERKKNVNKLPSSASRNVSDGK NVTSAKLTKIFVGKHEVY SGCGGALEVHKYNKAT TIPSGTTDDILGFYLISEKLLLYTASELILTTK YGRNMLHCADPPEVLRDFKPDTIINVLRLNKLFQVTLENGESWDYNFYEKTFTNVFSWKLRLKSIITKLNQSVALTSD R+YRSI  G P+EIK L GCQ I  S +++FV IFP  ICWV+WK+  I K+ +I VP+KS   Y  + +TVDV+N + TFQ  +G ++      +V+ +    EVTA+S+   +K+YVA++N++ L++    Q
Sbjct:   28 NVSDGNEFVNEIESQNATVYSRIMIGRSRNQSKRIERKKNVNKLPSSASRNVSDGKPNVTSAKLTKIFVGKHEVYTSGCGGALEVHKYNKATKTIPSGTTDDILGFYLISEKLLLYTASELILTTKLYGRNMLHCADPPEVLRDFKPDTIINVLRLNKLFQVTLENGESWDYNFYEKTFTNVFSWKLRLKSIITKLNQSVALTSDLRLYRSIGYGTPLEIKSLGGCQDIYPSSSEEFVVIFPTEICWVDWKTYIIKKRKKIYVPVKSFSTYCDMFMTVDVENTVKTFQKHKGSEM------VVRMYGRENEVTAISDHQSQKLYVAVNNQIKLIASPSYQ 361          
BLAST of SMED30023147 vs. Planmine SMEST
Match: SMESG000032765.1 (SMESG000032765.1)

HSP 1 Score: 359.762 bits (922), Expect = 1.219e-123
Identity = 174/196 (88.78%), Postives = 181/196 (92.35%), Query Frame = 3
Query:  789 DFKPDTIINVLRLNKLFQVTLENGESWDYNFYEKTFTNVFSWKLRLKSIITKLNQSVALTSDHRVYRSIDNGNPVEIKYLEGCQHISLSFNKQFVAIFPQTICWVNWKSCNIVKQTRIKVPIKSVFYSPLLLTVDVKNAIVTFQQGGQIRYPTGALVKEFPHNKEVTALSNVFFKKIYVAIDNEVHLLSRQPLQAA 1376
            DFKP++II V RL   FQ+ L NGE   +++     +NVFSWKLRLKSIITKLNQSVALTSDHRVYRSIDNGNPVEIKYLEGCQHISLSFNKQFVAIFPQTICWVNWKSCNIVKQTRIKVPIKSVFYSPLLLTVDVKNAIVTFQQGGQIRYPTGALVKEFPHNKEVTALSNVFFKKIYVAIDNEVHLLSRQPLQAA
Sbjct:    4 DFKPESIIAVHRLVNHFQIALGNGEICTFDYTRNKLSNVFSWKLRLKSIITKLNQSVALTSDHRVYRSIDNGNPVEIKYLEGCQHISLSFNKQFVAIFPQTICWVNWKSCNIVKQTRIKVPIKSVFYSPLLLTVDVKNAIVTFQQGGQIRYPTGALVKEFPHNKEVTALSNVFFKKIYVAIDNEVHLLSRQPLQAA 199          
BLAST of SMED30023147 vs. Planmine SMEST
Match: SMESG000053453.1 (SMESG000053453.1)

HSP 1 Score: 201.06 bits (510), Expect = 3.818e-59
Identity = 156/441 (35.37%), Postives = 237/441 (53.74%), Query Frame = 3
Query:   51 MHENESDQFQILLNSLNKIRVKEIEMKNKITFQEEEINKSLNFIDQFEKCLGDQHQMLSELNENAIEVTNNKGKISREIQCL----DDESEITNQIVIEQSEGQSKSNRNN-KNEFVNEIECQNATAYSRIMIGRSRNQSKRIERKKNVNKLPSSASRNVSDGKANVTSAKLTKIFVGKHEVYASGCGGALEVHKYNKATTTIPSGTTD-DILGFYLISEKLLLYTASELILTTKRYGRNMLHCADPPEVLRDFKPDTIINVLRLNKLFQVTLENGESWDYNFYEKTFTNVFSWKLRLKSIITKLNQSVALTSDHRVYRSIDNGNPVEIKYLEGCQHISLSFNKQFVAIFPQTICWVNWKSCNIVKQTRIKVPIKSV-FYSPLLLTVDVKNAIVTFQQGGQIRYPTGALVKEFPHNKEVTALSNVFFKKIYVAIDNEVHLL 1352
            M E  S  F+ LLN L +IR +EI+++NKI  Q+EEI K+ N I+QF+KC  +Q QMLS+L ++ I+   +K KI ++++ L    DDE +I N+ V EQ+  Q  +N  N ++E + E                      R+ RK+ + K  +         K ++   ++ KIF GK  + A G  G++   K  K T       TD DI  F+ I  K+LL T   LILT     +N +      ++++ F+P  I+++ R  K FQ+TL+NGE W Y F E  F    +    LK+IIT L++SVAL+ D +VY+  D  N  EIK LEGC  I    NK FVAIF   IC VN +   I  +  I  PIK +      L+TV+++N +  F     ++  +   +++F  N E+TA+ NV+ KKIY++++NE+  L
Sbjct:    1 MPETTSQIFKNLLNELVEIRDEEIKIENKIYIQQEEIIKTNNVIEQFQKCHVEQTQMLSKLKDDGIKFRISKNKILKKLRNLNHESDDEVDIQNENVTEQT--QLDNNECNLRDELIKE---------------------SRLTRKRKLKKNDNKDKYIKPMFKFDL--ERINKIFSGKPGIGACGSSGSIAFFK--KGTVKTMKAVTDKDIFDFFFIEGKILLQTEYLLILTE---FKNKVE-----KIVKGFQPWKILDICRNEKKFQITLQNGEIWIYEFPENIFLKYSTGIRNLKAIITYLSRSVALSLDQKVYQITDYKNVKEIKDLEGCDKIYKFCNKSFVAIFSTKICIVNLEDYKIQTEITINFPIKQLAICGSYLVTVNMENTVEKF----NVKNLSRKFIEQFSQNDEITAIPNVYQKKIYISVNNEICRL 402          
BLAST of SMED30023147 vs. Planmine SMEST
Match: SMESG000060288.1 (SMESG000060288.1)

HSP 1 Score: 73.1738 bits (178), Expect = 2.369e-15
Identity = 55/117 (47.01%), Postives = 82/117 (70.09%), Query Frame = 3
Query:   51 MHENESDQFQILLNSLNKIRVKEIEMKNKITFQEEEINKSLNFIDQFEKCLGDQHQMLSELNENAIEVTNNKGKISREIQCLD----DESEITNQIVIEQSEGQSKSNRNNKNEFVN 389
            M EN + +F+ LL +L +IR +EI+++NKI  Q++EINK+ N IDQF+KCL +Q QML++LN++ I+V   K KI R ++ LD    DE EI ++ VI Q   QS+ + NN+NE +N
Sbjct:    1 MSENTTQRFKNLLQNLVEIRDEEIKIENKIIIQQDEINKTNNIIDQFQKCLDEQTQMLAKLNDDGIQVKIRKNKILRRLRNLDHESEDEVEIPSENVIGQLADQSQLD-NNENELLN 116          
BLAST of SMED30023147 vs. Planmine SMEST
Match: SMESG000032764.1 (SMESG000032764.1)

HSP 1 Score: 71.633 bits (174), Expect = 4.105e-14
Identity = 50/122 (40.98%), Postives = 74/122 (60.66%), Query Frame = 3
Query:   51 MHENESDQFQILLNSLNKIRVKEIEMKNKITFQEEEINKSLNFIDQFEKCLGDQHQMLSELNENAIEVTNNKGKISREIQCLDDESEITNQIVIEQSEGQSKSNRNNKNEFVNEIECQNATA 416
            M E  S + +IL+N L +    E ++  KI+ Q+EEINK++N I +F++CL +Q+QM+S L EN      NK KI +EI+ LDDE +  N+I       QS+ + N +N F    ECQ+ TA
Sbjct:    1 MLEKGSQELEILMNRLIECNENEKKIITKISCQQEEINKTINIIQKFQQCLSEQYQMMSTLKENENVAKLNKKKIFQEIRNLDDEMQNVNEI------DQSQLDSNKENRFSIH-ECQSKTA 115          
The following BLAST results are available for this feature:
BLAST of SMED30023147 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023147 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023147 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023147 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023147 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023147 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023147 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023147 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023147 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023147 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023147 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023147 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023147 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30023147 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000032769.12.755e-17774.12SMESG000032769.1[more]
SMESG000032765.11.219e-12388.78SMESG000032765.1[more]
SMESG000053453.13.818e-5935.37SMESG000053453.1[more]
SMESG000060288.12.369e-1547.01SMESG000060288.1[more]
SMESG000032764.14.105e-1440.98SMESG000032764.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30023147 ID=SMED30023147|Name=SMED30023147|organism=Schmidtea mediterranea sexual|type=transcript|length=1512bp
AAATTTGTTATAAAAATCCTTATAAATAATAAATTAATAAATTAATTATC
ATGCATGAAAACGAATCTGACCAGTTTCAAATTTTATTAAATAGTTTAAA
TAAAATAAGAGTAAAAGAAATAGAAATGAAAAATAAAATCACTTTCCAAG
AAGAAGAAATCAATAAATCGCTGAATTTTATTGATCAGTTCGAAAAATGC
CTCGGGGATCAGCATCAGATGCTATCAGAATTGAACGAAAACGCGATAGA
AGTGACAAATAATAAAGGAAAAATTTCCAGAGAAATTCAATGTTTGGATG
ATGAAAGCGAAATTACCAATCAAATCGTGATTGAGCAATCCGAAGGCCAA
TCAAAATCGAACAGAAACAATAAAAATGAATTTGTGAATGAAATCGAATG
CCAAAATGCAACTGCATACAGTAGAATTATGATTGGACGATCAAGAAACC
AATCAAAACGTATTGAGAGGAAAAAAAATGTGAATAAATTGCCATCGTCA
GCAAGTAGGAATGTCTCAGATGGAAAAGCCAACGTGACAAGCGCAAAACT
AACGAAAATATTTGTAGGGAAACATGAAGTGTACGCATCCGGTTGTGGAG
GAGCTCTCGAGGTCCATAAATATAATAAAGCTACCACGACAATTCCATCC
GGGACAACGGACGATATCCTCGGTTTCTATTTGATTAGTGAGAAACTATT
GCTGTATACGGCAAGCGAGCTGATTCTGACCACAAAACGATACGGGAGAA
ATATGCTGCATTGTGCAGATCCCCCGGAAGTTCTCAGAGACTTCAAGCCG
GACACCATCATAAATGTCCTCCGACTGAATAAACTCTTTCAAGTGACTCT
AGAAAACGGCGAGAGCTGGGATTATAATTTCTATGAAAAAACGTTTACAA
ATGTTTTCTCCTGGAAATTGAGATTGAAATCGATAATCACAAAACTCAAT
CAGTCCGTGGCACTCACATCGGATCACAGGGTTTATCGCTCAATCGATAA
TGGAAATCCAGTGGAAATAAAATACCTGGAAGGTTGCCAGCACATTTCTT
TATCTTTCAATAAGCAATTTGTTGCGATTTTCCCACAGACAATTTGCTGG
GTTAATTGGAAGAGCTGCAATATTGTCAAGCAAACAAGAATAAAAGTCCC
AATCAAAAGCGTATTCTATTCACCCCTGTTGCTGACAGTCGATGTCAAGA
ACGCAATCGTGACATTCCAGCAAGGGGGGCAAATCAGATATCCCACCGGA
GCTCTGGTGAAGGAATTCCCTCATAATAAGGAAGTCACTGCACTTTCAAA
TGTTTTCTTTAAGAAGATTTATGTAGCCATTGACAACGAGGTCCACCTGC
TCAGCCGGCAGCCCCTGCAAGCGGCATGAGAGATTTCCCAAGAGATTTAC
TGAATAAATTTTGCTATATTATGATTATAGATATCTATGTAACTTTTTGA
GTTGTCAATGCGCAAATTGATCTATTGTTTACACATTTATATTTCAATAT
TGACTTTACGCT
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protein sequence of SMED30023147-orf-1

>SMED30023147-orf-1 ID=SMED30023147-orf-1|Name=SMED30023147-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=443bp
MHENESDQFQILLNSLNKIRVKEIEMKNKITFQEEEINKSLNFIDQFEKC
LGDQHQMLSELNENAIEVTNNKGKISREIQCLDDESEITNQIVIEQSEGQ
SKSNRNNKNEFVNEIECQNATAYSRIMIGRSRNQSKRIERKKNVNKLPSS
ASRNVSDGKANVTSAKLTKIFVGKHEVYASGCGGALEVHKYNKATTTIPS
GTTDDILGFYLISEKLLLYTASELILTTKRYGRNMLHCADPPEVLRDFKP
DTIINVLRLNKLFQVTLENGESWDYNFYEKTFTNVFSWKLRLKSIITKLN
QSVALTSDHRVYRSIDNGNPVEIKYLEGCQHISLSFNKQFVAIFPQTICW
VNWKSCNIVKQTRIKVPIKSVFYSPLLLTVDVKNAIVTFQQGGQIRYPTG
ALVKEFPHNKEVTALSNVFFKKIYVAIDNEVHLLSRQPLQAA*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000014zeta neoblast
PLANA:0000101muscle cell
PLANA:0000429neoblast
PLANA:0002109X1 cell
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 134..159