Cell wall integrity and stress response component 1

Overview
NameCell wall integrity and stress response component 1
Smed IDSMED30023146
Length (bp)983
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Cell wall integrity and stress response component 1 (SMED30023146) t-SNE clustered cells

Violin plots show distribution of expression levels for Cell wall integrity and stress response component 1 (SMED30023146) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Cell wall integrity and stress response component 1 (SMED30023146) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Cell wall integrity and stress response component 1 (SMED30023146) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30023146

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 6

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
gutSMED30023146h1SMcG0020062 dd_Smed_v4_1356_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30023146h1SMcG0020062 dd_Smed_v4_1356_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30023146h1SMcG0020062 dd_Smed_v4_1356_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30023146h1SMcG0020062 dd_Smed_v4_1356_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
body wall musculatureSMED30023146h1SMcG0020062 dd_Smed_v4_1356_0_1dd_Smed_v4PMID:30471994
Scimone et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30023146h1SMcG0020062 dd_Smed_v4_1356_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Cell wall integrity and stress response component 1 vs. TrEMBL
Match: A0A5P8I4L8 (Uncharacterized protein OS=Schmidtea mediterranea OX=79327 PE=2 SV=1)

HSP 1 Score: 252.677 bits (644), Expect = 1.105e-79
Identity = 122/247 (49.39%), Postives = 172/247 (69.64%), Query Frame = 1 
Query:  133 MKNSKTKLLVIGAGQMRTGTTSLKSALEKLYGGKIYHMNEIRFNGVDHIGLWIKALDQGHLDREISEEIFTGYCAAVDNPTATFYQDLIEIYPDAKVILTTRNSDDWVKSVRSTIIRKGPIMNILEKISLWNQGIFRFEELCVKLRWHSFHVPYEILQYDDEVLKESFEKYNERVRQTVPKERLLEFNAKDGWTPLCEFLDLPIPNEPFPFVNNSEEFIDKIKSDITKTLVKFGSITVLLTFGILHL 873
            M + K ++LVIGAGQMRTGT SLK ALEKLY    YHM EI  +  DH+ LWI   D G ++ + + +IF+ Y A VD P+A  Y+DL+EIYP+AKV+LTTRNSDDWVKSV+ST +R     + + KI  +      F  +  K+   ++  P++ L+ DDE+LK+ +E++NE VR+ VPKERLLEFNAK+GW PLC FL+LP+PNEP+PFVN +++F   ++  + K++++ G + + LT G L L
Sbjct:    1 MNHRKNEILVIGAGQMRTGTFSLKFALEKLYNRPCYHMAEILNHHKDHVKLWISVFDSGKMNEKTAGKIFSDYSATVDFPSAAVYKDLMEIYPNAKVVLTTRNSDDWVKSVKSTTLRNNMKTSFVHKILFYFNLGTEFPVMLDKMYLKAYKEPFDALRTDDELLKQCYEEFNEEVRKYVPKERLLEFNAKEGWEPLCRFLNLPVPNEPYPFVNTTKDFQRNVRGMMLKSVIRSG-LVLSLTIGCLSL 246
[Close]
BLAST of Cell wall integrity and stress response component 1 vs. TrEMBL
Match: G7YDV6 (NAD dependent epimerase/dehydratase OS=Clonorchis sinensis OX=79923 GN=CLF_105639 PE=4 SV=1)

HSP 1 Score: 182.185 bits (461), Expect = 4.745e-52
Identity = 102/249 (40.96%), Postives = 142/249 (57.03%), Query Frame = 1 
Query:  145 KTKLLVIGAGQMRTGTTSLKSALEKLYGGKIYHMNEIRFNGVDHIGLWI---KALDQ---GHLDREISEEIFTGYCAAVDNPTATFYQDLIEIYPDAKVILTTRNSDDWVKSVRSTIIRKGPI--MNILEKISLWNQGIFRFEELCVKLRWHSFHVPYEILQYDDEVLKESFEKYNERVRQTVPKERLLEFNAKDGWTPLCEFLDLPIPNEPFPFVNNSEEFIDKIKSDITKTLVKFGSITVLLTFGIL 867
            K  +LVIGAG  RTGT SLKSALE +YG   YHM E+     DH   W+   K + Q   GH++  + +EIF GY   VD P  T+Y  L+E YPDAKVILT R +  W+ SVR TI++K     MN LE+   +     +  +  +K       + Y + +Y D+    +FEK+N+RV++ VP ERLL F   DGW PLC+FL +P+P  PFP  NN EEF   +   + +  ++   I + L  G++
Sbjct:   11 KPPILVIGAGLGRTGTMSLKSALEHIYGEPCYHMFELLSQHRDHAQKWLQVDKLVSQSPDGHIEGSLFDEIFKGYRCTVDYPACTYYAQLMEHYPDAKVILTVRETTKWLDSVRHTILKKRTFSWMNWLEEFLQFGAATVQMFDCEIKRS-----LGYTVDRYSDKECAAAFEKWNQRVQRYVPAERLLVFRVTDGWEPLCQFLQVPVPTIPFPHKNNREEFHSNVNFHMDQHRIQ---ILLFLAMGLV 251
[Close]
BLAST of Cell wall integrity and stress response component 1 vs. TrEMBL
Match: A0A267F2Y8 (Uncharacterized protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig019790g1 PE=4 SV=1)

HSP 1 Score: 179.489 bits (454), Expect = 3.539e-51
Identity = 91/220 (41.36%), Postives = 137/220 (62.27%), Query Frame = 1 
Query:  142 SKTKLLVIGAGQMRTGTTSLKSALEKLYGGKIYHMNEIRFNGVDHIGLWIKALDQGHLDREISEEIFTGYCAAVDNPTATFYQDLIEIYPDAKVILTTRNSDDWVKSVRSTIIRKGPIMNILEKISLWNQGIFRFEELCVKLRWHSFHVPYEILQYDDEVLKESFEKYNERVRQTVPKERLLEFNAKDGWTPLCEFLDLPIP-NEPFPFVNNSEEFIDKI 798
            S   L VIGAG MRTGT S   ALE L GG+ YHM+ +    + H+ LW +A  QG L +E++EEIF  + A  D P A FY++L++IYPDAK +L+ R+  DWV+SVR+TI++    +  +     W  G+  +  L   +        +++    D  + ++F ++N+ V +T+PKERLL F   DGW PLC+FL+LP+P NEPFP +N+++ F++ I
Sbjct:    2 SSAGLRVIGAGLMRTGTNSQMKALEMLLGGRCYHMDIVMEEKLSHLQLWHRAAIQG-LSKELAEEIFDDFLACTDFPAAHFYKELMQIYPDAKFVLSIRDPTDWVRSVRATILQSKLTVEYMLPPVRWLLGLSHYLPLSDAVLGSRLGFNFDM---SDAEMIDAFNRHNDTVIRTIPKERLLVFRVSDGWEPLCKFLELPVPSNEPFPHLNDTKRFLETI 217
[Close]
BLAST of Cell wall integrity and stress response component 1 vs. TrEMBL
Match: A0A267EKC2 (Uncharacterized protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig019766g3 PE=4 SV=1)

HSP 1 Score: 179.489 bits (454), Expect = 5.597e-51
Identity = 91/220 (41.36%), Postives = 137/220 (62.27%), Query Frame = 1 
Query:  130 KMKNSKTKLLVIGAGQMRTGTTSLKSALEKLYGGKIYHMNEIR--FNGVDHIGLWIKALDQGHLDREISEEIFTGYCAAVDNPTATFYQDLIEIYPDAKVILTTRNSDDWVKSVRSTIIRKGPIMNILEKISLWNQGIFRFEELCVKLRWHSFHVPYEILQYDDEVLKESFEKYNERVRQTVPKERLLEFNAKDGWTPLCEFLDLPIPNEPFPFVNNSEE 783
            KM      L+VIGAG  RTGT S K ALE L G   YHM E     N   HI +WI+A++ G L + +++EI   Y A VD PTA+FY++L++ YP+AKVIL+ R+++ WV+S++S+I++    M    +   W  GI + +++   +    F   Y +   D E +   FE+ N+ +R+ VP ERLLEF   DGW PLC+FL+LP+P++PFP +N+ ++
Sbjct:   11 KMPQKTHSLVVIGAGAPRTGTVSQKVALELLLGQPCYHMGEALGLHNSRRHIPMWIQAVESG-LSKSLADEILGNYSACVDYPTASFYKELMQHYPNAKVILSVRDTNSWVESMQSSILKAMHTMERFPRPLKWLLGIGQADKMIDAVFRREFGDNYNLA--DSEFMARQFEERNDSIRRVVPSERLLEFRVSDGWAPLCQFLNLPVPDKPFPRLNDRKQ 227
[Close]
BLAST of Cell wall integrity and stress response component 1 vs. TrEMBL
Match: A0A267EUL9 (Uncharacterized protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig019766g2 PE=4 SV=1)

HSP 1 Score: 179.104 bits (453), Expect = 6.320e-51
Identity = 91/220 (41.36%), Postives = 136/220 (61.82%), Query Frame = 1 
Query:  130 KMKNSKTKLLVIGAGQMRTGTTSLKSALEKLYGGKIYHMNEI--RFNGVDHIGLWIKALDQGHLDREISEEIFTGYCAAVDNPTATFYQDLIEIYPDAKVILTTRNSDDWVKSVRSTIIRKGPIMNILEKISLWNQGIFRFEELCVKLRWHSFHVPYEILQYDDEVLKESFEKYNERVRQTVPKERLLEFNAKDGWTPLCEFLDLPIPNEPFPFVNNSEE 783
            KM      L+VIGAG  RTGT S K ALE L G   YHM E     N   HI +WI+A++ G L + +++EI   Y A VD PTA+FY++L++ YP+AKVIL+ R++  WV+S++S+I++    M    +   W  GI + +++   +    F   Y +   D E +   FE+ N+ +R+ VP ERLLEF   DGW PLC+FL+LP+P++PFP +N+ ++
Sbjct:   10 KMPQKTHSLVVIGAGAPRTGTVSQKVALELLLGQPCYHMGEALGPHNSRRHIPMWIQAVESG-LSKSLADEILGNYAACVDYPTASFYKELMQHYPNAKVILSVRDTSSWVESMQSSILKAMHTMERFPRPLKWLLGIGQADKMIDAVFRREFGDNYNLA--DSEFMARRFEERNDSIRRVVPSERLLEFRVSDGWAPLCQFLNLPVPDKPFPRLNDRKQ 226
[Close]
BLAST of Cell wall integrity and stress response component 1 vs. Ensembl Nematostella
Match: EDO48574 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RIV4])

HSP 1 Score: 97.0561 bits (240), Expect = 8.011e-23
Identity = 70/217 (32.26%), Postives = 108/217 (49.77%), Query Frame = 1 
Query:  157 LVIGAGQMRTGTTSLKSALEKLYGGKIYHMNEIRFNGVDHIGLWIKALDQGHLDREISEEIFTGYCAAVDNPTATFYQDLIEIYPDAKVILTTRNSDD-WVKSVRSTIIRKGPIMNILEKISLWNQGIFRFEELCVKLRWHSFHVPYEILQYDDEVLKE--SFEKYNERVRQTVPKERLLEFNAKDGWTPLCEFLDLPIPNEPFPFVNNSEEFIDKI 798
            L+IGAG  +TGT SL SAL  L      H  +I+F+  ++I  +     +G +     EE++    A  D P + FY+++ + +PDAKVIL+ R+ ++ W +S+  T+     + N        N  +     L    R +S      IL++    LK    + K+N  V   +P ++LL FN KDGW PLC FL   +P  P+P VN +   I  I
Sbjct:  157 LIIGAGLPKTGTKSLASALRHLGYNVHDHPEQIQFHCDEYIAAF-----EGQMPN--FEEMYANVDATTDLPASIFYKEIFKAFPDAKVILSVRDDEESWRRSLLKTVGVVSSMTN--------NPLLIVCRTLTPTGRKYS-----RILRFIMNYLKNEIDYTKHNGDVMANIPAKQLLVFNPKDGWEPLCAFLGAEVPAIPYPRVNVNSTAIPSI 353
[Close]
BLAST of Cell wall integrity and stress response component 1 vs. Ensembl Nematostella
Match: EDO38503 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SCW7])

HSP 1 Score: 91.2781 bits (225), Expect = 9.402e-22
Identity = 68/213 (31.92%), Postives = 106/213 (49.77%), Query Frame = 1 
Query:  163 IGAGQMRTGTTSLKSALEKLYGGKIYHMNEIRFNGVDHIGLWIKALDQGHLDREISEEIFTGYCAAVDNPTATFYQDLIEIYPDAKVILTTR-NSDDWVKSVRSTIIRKGPIMNILEKISLWNQGIFRFEELCVKL--RWHSFHVPYEILQYDDEVLKE-SFEKYNERVRQTVPKERLLEFNAKDGWTPLCEFLDLPIPNEPFPFVNNSEEFI 789
            I AG  +TGT SL +AL  L G  +Y + E   N   H+  WIKA    + D      ++     A+D P   F +D++   P+ KVILT R N+++W+ S+  +       ++I    S++NQ   +   +      +W +      ++++    LK   + ++ E V+  VP +RLL FN K+GW PLC FL    P+ PFP  N   EFI
Sbjct:    4 ICAGLSKTGTKSLVAALRYL-GYTVYDIEE---NWRYHMDEWIKAFQGTNPD---FTAMYASVDTAIDLPPYLFVRDILVALPELKVILTVRENAEEWLTSLTRS-------LDIWW--SVYNQRWLKLARVLTPTGRKWAT------LIRHQTAALKTLDYTRHIEEVKAIVPADRLLVFNVKEGWEPLCAFLGHEAPSIPFPHRNKKSEFI 194
[Close]
BLAST of Cell wall integrity and stress response component 1 vs. Ensembl Nematostella
Match: EDO39565 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S9S8])

HSP 1 Score: 91.2781 bits (225), Expect = 1.297e-21
Identity = 77/254 (30.31%), Postives = 125/254 (49.21%), Query Frame = 1 
Query:  160 VIGAGQMRTGTTSLKSALEKLYGGKIYHMNEIRFNGVDHIGLWIKALDQGHLDREISEEIFTGYCAAVDNPTATFYQDLIEIYPDAKVILTTRNSDD-WVKS-VRSTIIRKGPIMNILEKISLWNQGIFRFEELCVKLRWHSFHVPYEILQYDDEVLKESFEKYNERVRQTVPKERLLEFNAKDGWTPLCEFLDLPIPNEPFPFVN-NSEEFIDKIKSD-----ITKTLVKFGSITVLL---TFGILHL--FRY 882
            +IGAG  +TGT SL SAL +L      H  +++F+  ++I  +   +          EE++    A  D P   FY+++ + +PDAKVIL+ R+ ++ W KS +++  I      N+L  + L+     R      KL    + + Y + +Y    L   + K+N  V  T+P ++LL FN KDGW  LC FL   +P  P+P  N NS      IK+      + + L    ++ V+L   T+ +L L  F+Y
Sbjct:    3 IIGAGLPKTGTKSLASALRRLGFNVHDHHEQLQFHCDEYIAAFKGPMPN-------FEEMYENVDATTDLPACIFYKEIFKAFPDAKVILSVRDDEESWRKSFLKTESIVSSATSNLLMILGLFLTPSGR------KL----WRLGYNVKKYVKNEL--DYTKHNNEVMATIPAKQLLVFNPKDGWESLCAFLGAEVPAIPYPRENVNSTGVPSTIKNHWHVRRMKRELAVMMAVFVVLLGCTYALLSLPYFKY 237
[Close]
BLAST of Cell wall integrity and stress response component 1 vs. Ensembl Nematostella
Match: EDO48573 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RIV3])

HSP 1 Score: 90.8929 bits (224), Expect = 1.622e-21
Identity = 66/220 (30.00%), Postives = 109/220 (49.55%), Query Frame = 1 
Query:  160 VIGAGQMRTGTTSLKSALEKLYGGKIYHMNE-IRFNGVDHIGLWIKALDQGHLDREISEEIFTGYCAAVDNPTATFYQDLIEIYPDAKVILTTR-NSDDWVKSVRSTI-----IRKGPIMNILEKISLWNQGIFRFEELCVKLRWHSFHVPYEILQYDDEVLKESFEKYNERVRQTVPKERLLEFNAKDGWTPLCEFLDLPIPNEPFPFVNNSEEFIDKI 798
            +IGAG  +TGT SL SAL  L G  ++   E ++F+  ++I  +     +G +     EE++    A  D P   FY+D+   +PDAKVIL+ R N + W +S   T+     +   P++ +   ++   +   RF               + +++Y +  L   ++K+N+ V   +  ++LL FN KDGW PLC FL   +P  P+P VN +   I  I
Sbjct:    3 IIGAGLPKTGTKSLASALRHL-GYNVHDRPEQVQFHCDEYIAAF-----EGQMPN--FEEMYENVDATTDVPACLFYKDIFRAFPDAKVILSVRDNEESWRRSFLKTLEVLSSMINNPLIILGMLLTPSGRKFSRFR--------------HNLIKYVENEL--DYKKHNDAVMANIQAKQLLVFNPKDGWEPLCAFLGAEVPTIPYPRVNVNSTAIPSI 198
[Close]
BLAST of Cell wall integrity and stress response component 1 vs. Ensembl Nematostella
Match: EDO49231 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RGU4])

HSP 1 Score: 91.2781 bits (225), Expect = 1.701e-21
Identity = 64/214 (29.91%), Postives = 104/214 (48.60%), Query Frame = 1 
Query:  160 VIGAGQMRTGTTSLKSALEKLYGGKIYHMNEIRFNGVDHIGLWIKALDQGHLDREISEEIFTGYCAAVDNPTATFYQDLIEIYPDAKVILTTRNSDDWVKSVRSTIIRKGPIMNILEKISL--WNQGIFRFEELCVKLRWHSFHVPYEILQYDDEVLKESFEKYNERVRQTVPKERLLEFNAKDGWTPLCEFLDLPIPNEPFPFVNNSEEFIDK 795
            VI AG  +T T S+  AL ++ G  +Y + E      D    W+  ++ G L     E +F    A  D P   F++++++  PDAKVILT R  + W++S +  +     + + L  +SL     GI +     V   + + +           ++ ++ E +N+RVR TVP  +LLE++  +GW PLC+FL    P E FP  N   EF  +
Sbjct:    3 VICAGICKTATKSMAKAL-RILGYNVYDVEEQICELFDD---WLDLIEHGRLPD--FEAMFENVDAITDLPGNFFFEEILKASPDAKVILTVREQEAWLRSSKKQLKMVQGLFSKLWFLSLSPIYLGIRKITNGMVAALYGTINAE------STWIIAKALEMHNQRVRSTVPPGQLLEYSVTEGWGPLCDFLGHATPKEKFPHENVKGEFTKR 204
[Close]
BLAST of Cell wall integrity and stress response component 1 vs. Planmine SMEST
Match: SMESG000035067.1 (SMESG000035067.1)

HSP 1 Score: 493.426 bits (1269), Expect = 3.479e-178
Identity = 238/253 (94.07%), Postives = 243/253 (96.05%), Query Frame = 1 
Query:  133 MKNSKTKLLVIGAGQMRTGTTSLKSALEKLYGGKIYHMNEIRFNGVDHIGLWIKALDQGHLDREISEEIFTGYCAAVDNPTATFYQDLIEIYPDAKVILTTRNSDDWVKSVRSTIIRKGPIMNILEKISLWNQGIFRFEELCVKLRWHSFHVPYEILQYDDEVLKESFEKYNERVRQTVPKERLLEFNAKDGWTPLCEFLDLPIPNEPFPFVNNSEEFIDKIKSDITKTLVKFGSITVLLTFGILHLFRYVRP 891
            M     KL VIGAGQMRTGTTSLKSALEKLYGGK+YHM+EI FNGVDHIGLWIKALDQGHL +E+SEEIFTGYCAAVDNPTATFYQDLIEIYPDAKVILTTRNSDDWVKSVRSTIIRKGPIMNILEKISLWNQ IFRFEELCVKLRWHSFHVPYEILQYDDEVLKESFEKYNERVRQTVPKERLLEFNAKDGWTPLCEFLDLPIPNEPFPFVNNSEEF+DKIKSDITKTLVKFGSITVLLTFGILHLFRY RP
Sbjct:    1 MTADSNKLFVIGAGQMRTGTTSLKSALEKLYGGKVYHMDEILFNGVDHIGLWIKALDQGHLCKELSEEIFTGYCAAVDNPTATFYQDLIEIYPDAKVILTTRNSDDWVKSVRSTIIRKGPIMNILEKISLWNQRIFRFEELCVKLRWHSFHVPYEILQYDDEVLKESFEKYNERVRQTVPKERLLEFNAKDGWTPLCEFLDLPIPNEPFPFVNNSEEFMDKIKSDITKTLVKFGSITVLLTFGILHLFRYGRP 253
[Close]
BLAST of Cell wall integrity and stress response component 1 vs. Planmine SMEST
Match: SMESG000035062.1 (SMESG000035062.1)

HSP 1 Score: 490.73 bits (1262), Expect = 3.150e-177
Identity = 237/253 (93.68%), Postives = 243/253 (96.05%), Query Frame = 1 
Query:  133 MKNSKTKLLVIGAGQMRTGTTSLKSALEKLYGGKIYHMNEIRFNGVDHIGLWIKALDQGHLDREISEEIFTGYCAAVDNPTATFYQDLIEIYPDAKVILTTRNSDDWVKSVRSTIIRKGPIMNILEKISLWNQGIFRFEELCVKLRWHSFHVPYEILQYDDEVLKESFEKYNERVRQTVPKERLLEFNAKDGWTPLCEFLDLPIPNEPFPFVNNSEEFIDKIKSDITKTLVKFGSITVLLTFGILHLFRYVRP 891
            M     KL VIGAGQMRTGTTSLKSALEKLYGGK+YHM+EI FNGVDHIGLWIKALDQGHL +E+SEEIFTGYCAAVDNPTATFYQDLIEIYPDAKVILTTRNSDDWVKSVRSTIIRKGPIMNILEKISLWNQ IFRFEELCVKLRWHSFHVPYEILQYDDEVLKESFEKYNERVRQTVPKERLLEFNAKDGWTPLCEFLDLPIPNEPFPFVNNSEEF+DKIKSDITKTLVKFGSITVLLTFGIL+LFRY RP
Sbjct:    1 MTADSNKLFVIGAGQMRTGTTSLKSALEKLYGGKVYHMDEILFNGVDHIGLWIKALDQGHLCKELSEEIFTGYCAAVDNPTATFYQDLIEIYPDAKVILTTRNSDDWVKSVRSTIIRKGPIMNILEKISLWNQRIFRFEELCVKLRWHSFHVPYEILQYDDEVLKESFEKYNERVRQTVPKERLLEFNAKDGWTPLCEFLDLPIPNEPFPFVNNSEEFMDKIKSDITKTLVKFGSITVLLTFGILYLFRYGRP 253
[Close]
BLAST of Cell wall integrity and stress response component 1 vs. Planmine SMEST
Match: SMESG000035061.1 (SMESG000035061.1)

HSP 1 Score: 410.609 bits (1054), Expect = 1.345e-145
Identity = 194/243 (79.84%), Postives = 215/243 (88.48%), Query Frame = 1 
Query:  133 MKNSKTKLLVIGAGQMRTGTTSLKSALEKLYGGKIYHMNEIRFNGVDHIGLWIKALDQGHLDREISEEIFTGYCAAVDNPTATFYQDLIEIYPDAKVILTTRNSDDWVKSVRSTIIRKGPIMNILEKISLWNQGIFRFEELCVKLRWHSFHVPYEILQYDDEVLKESFEKYNERVRQTVPKERLLEFNAKDGWTPLCEFLDLPIPNEPFPFVNNSEEFIDKIKSDITKTLVKFGSITVLLTFG 861
            MKNSKTKLLVIGAGQMRTGTTSLKSALEKLYGGKIYHMNEIRFNGVDHIGLWIKALDQGHLDREISEEIFTGYCAAVDNPTATFYQDLIEIYPDAKVILTTRNSDDWVKSV+ T I K  +   LE+  LWN+GI + E++C+K+RWHSF VPY+ILQ DD+VLKESFEKYNE+VRQTVPKERLLEFN KDGW PLCEFLDLPIPNE FPFVNN++E+   +K +I +TL K+ S+ V+L   
Sbjct:    1 MKNSKTKLLVIGAGQMRTGTTSLKSALEKLYGGKIYHMNEIRFNGVDHIGLWIKALDQGHLDREISEEIFTGYCAAVDNPTATFYQDLIEIYPDAKVILTTRNSDDWVKSVKQTTIIKNGLQLSLERFCLWNKGISKLEQMCMKIRWHSFQVPYKILQNDDKVLKESFEKYNEKVRQTVPKERLLEFNPKDGWKPLCEFLDLPIPNESFPFVNNADEYDKNVKMEIFQTLAKYVSVIVILMIA 243
[Close]
BLAST of Cell wall integrity and stress response component 1 vs. Planmine SMEST
Match: SMESG000035059.1 (SMESG000035059.1)

HSP 1 Score: 386.726 bits (992), Expect = 4.260e-136
Identity = 181/249 (72.69%), Postives = 210/249 (84.34%), Query Frame = 1 
Query:  133 MKNSKTKLLVIGAGQMRTGTTSLKSALEKLYGGKIYHMNEIRFNGVDHIGLWIKALDQGHLDREISEEIFTGYCAAVDNPTATFYQDLIEIYPDAKVILTTRNSDDWVKSVRSTIIRKGPIMNILEKISLWNQGIFRFEELCVKLRWHSFHVPYEILQYDDEVLKESFEKYNERVRQTVPKERLLEFNAKDGWTPLCEFLDLPIPNEPFPFVNNSEEFIDKIKSDITKTLVKFGSITVLLTFGILHLFR 879
            M N+   L VIGAGQMRTGTTSLK ALE+LYGGKIYHM+E+ FNGVDHIGLWI+ALD+GHLDRE+S EIFTGYCAAV NPTA+FYQDLIEIYPDAKVILTTRNSDDW++SVR T +RK  +M ILE+ISLWN+GIFRFEELCVKLRWHSFH+ ++ LQ DDE+LKESFE++NE+VR TV KERLLEF  KDGW PLC+FLDLPIPNEPFPFVNNSEEF  +I     K L+K+  + V+    I +L +
Sbjct:    1 MPNNGQNLFVIGAGQMRTGTTSLKYALERLYGGKIYHMDELYFNGVDHIGLWIRALDKGHLDRELSGEIFTGYCAAVGNPTASFYQDLIEIYPDAKVILTTRNSDDWIRSVRKTTMRKSELMKILERISLWNKGIFRFEELCVKLRWHSFHLQFDTLQNDDELLKESFERFNEKVRLTVSKERLLEFKPKDGWKPLCQFLDLPIPNEPFPFVNNSEEFSKRIVRSYVKDLLKYILLIVVCGLSICYLLK 249
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BLAST of Cell wall integrity and stress response component 1 vs. Planmine SMEST
Match: SMESG000035040.1 (SMESG000035040.1)

HSP 1 Score: 265.388 bits (677), Expect = 3.048e-88
Identity = 128/244 (52.46%), Postives = 164/244 (67.21%), Query Frame = 1 
Query:  133 MKNSKTKLLVIGAGQMRTGTTSLKSALEKLYGGKIYHMNEIRFNGVDHIGLWIKALDQGHLDREISEEIFTGYCAAVDNPTATFYQDLIEIYPDAKVILTTRNSDDWVKSVRSTIIRKGPIMNILEKISLWNQGIFRFEELCVKLRWHSFHVPYEILQYDDEVLKESFEKYNERVRQTVPKERLLEFNAKDGWTPLCEFLDLPIPNEPFPFVNNSEEFIDKIKSDITKTLVKFGSITVLLTFGI 864
            M+    +L VIGAGQMRTGT SLK ALEKLY GK YHM EI  N  +HI LWI   D+  LD+  +E + + YCA VD P+   Y+DL+++YP+AKVILTTRNSDDW+KSVR+T ++KG  +  L KI     G+ RF E   KL  H++   YE L  DD +LK+ +E +N+ VR+TVPKERLLEFN+K GW PLC FL+LPIP+EP+PFVN++EEF   I        VK+G    +    I
Sbjct:    1 MRKKNHELSVIGAGQMRTGTKSLKMALEKLYNGKCYHMAEIFENHRNHISLWISVFDKNGLDKSTAESLLSDYCATVDFPSTAVYKDLMKLYPNAKVILTTRNSDDWLKSVRNTTLKKGSDVTFLSKIFFMFSGLGRFPEFSEKLINHAYKNSYETLINDDAILKQCYEDFNQEVRETVPKERLLEFNSKQGWEPLCTFLNLPIPDEPYPFVNSTEEFNKYINKTYLADFVKYGIFVTIFAGAI 244
The following BLAST results are available for this feature:
BLAST of Cell wall integrity and stress response component 1 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Cell wall integrity and stress response component 1 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Cell wall integrity and stress response component 1 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Cell wall integrity and stress response component 1 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Cell wall integrity and stress response component 1 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Cell wall integrity and stress response component 1 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Cell wall integrity and stress response component 1 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Cell wall integrity and stress response component 1 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A5P8I4L81.105e-7949.39Uncharacterized protein OS=Schmidtea mediterranea ... [more]
G7YDV64.745e-5240.96NAD dependent epimerase/dehydratase OS=Clonorchis ... [more]
A0A267F2Y83.539e-5141.36Uncharacterized protein (Fragment) OS=Macrostomum ... [more]
A0A267EKC25.597e-5141.36Uncharacterized protein (Fragment) OS=Macrostomum ... [more]
A0A267EUL96.320e-5141.36Uncharacterized protein (Fragment) OS=Macrostomum ... [more]
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BLAST of Cell wall integrity and stress response component 1 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Cell wall integrity and stress response component 1 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Cell wall integrity and stress response component 1 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Cell wall integrity and stress response component 1 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO485748.011e-2332.26Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO385039.402e-2231.92Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO395651.297e-2130.31Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO485731.622e-2130.00Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO492311.701e-2129.91Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Cell wall integrity and stress response component 1 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Cell wall integrity and stress response component 1 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000035067.13.479e-17894.07SMESG000035067.1[more]
SMESG000035062.13.150e-17793.68SMESG000035062.1[more]
SMESG000035061.11.345e-14579.84SMESG000035061.1[more]
SMESG000035059.14.260e-13672.69SMESG000035059.1[more]
SMESG000035040.13.048e-8852.46SMESG000035040.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30023146 ID=SMED30023146|Name=Cell wall integrity and stress response component 1|organism=Schmidtea mediterranea sexual|type=transcript|length=983bp
AATATTGTGTACAGCTCTTGCTAAACAACAGAACTTGAAAGGTTGAAAAA
CAAGATCAATTTATAGATAAATATATGCCAACAAATATGTAGAGGTATAT
ATAATGAGCCGAATAATAATTGGTCTTTCAAAATGAAAAATAGCAAAACC
AAATTATTGGTGATCGGAGCGGGTCAAATGAGAACCGGAACTACATCCTT
AAAATCGGCATTAGAAAAGCTGTATGGAGGTAAAATTTATCATATGAATG
AAATTCGATTCAATGGCGTTGATCACATCGGTCTCTGGATAAAGGCTTTA
GATCAAGGTCATTTGGATAGAGAGATTTCAGAAGAAATCTTTACCGGATA
CTGCGCTGCTGTTGACAATCCTACAGCAACGTTTTATCAAGATCTAATCG
AGATCTATCCCGATGCAAAAGTGATATTAACAACACGAAATTCTGATGAT
TGGGTAAAGAGTGTAAGAAGCACAATTATTCGAAAAGGACCAATAATGAA
CATTTTGGAAAAGATTAGCTTATGGAATCAAGGGATTTTTCGGTTTGAAG
AATTATGTGTAAAATTACGATGGCATTCATTTCATGTGCCATACGAAATT
CTACAGTATGACGACGAAGTCCTAAAAGAGTCATTTGAAAAATATAACGA
AAGGGTGAGACAAACGGTTCCTAAAGAGAGATTGTTAGAATTCAACGCCA
AAGATGGTTGGACACCTTTATGTGAATTTTTAGATTTACCGATTCCTAAT
GAACCGTTTCCATTCGTCAACAATTCTGAAGAATTTATCGATAAAATAAA
AAGTGACATAACAAAAACATTGGTCAAGTTTGGATCTATAACTGTGTTGT
TGACTTTTGGCATTCTACATTTGTTTAGATATGTCAGACCCTAACTTTAT
TTTAATTTTTATTTATTTCTGAGAAAATAATAGATATAGGTACAATAAAT
GAGTTTTCTAAATTTTGTTTATTAATAATGTTA
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protein sequence of SMED30023146-orf-1

>SMED30023146-orf-1 ID=SMED30023146-orf-1|Name=SMED30023146-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=254bp
MKNSKTKLLVIGAGQMRTGTTSLKSALEKLYGGKIYHMNEIRFNGVDHIG
LWIKALDQGHLDREISEEIFTGYCAAVDNPTATFYQDLIEIYPDAKVILT
TRNSDDWVKSVRSTIIRKGPIMNILEKISLWNQGIFRFEELCVKLRWHSF
HVPYEILQYDDEVLKESFEKYNERVRQTVPKERLLEFNAKDGWTPLCEFL
DLPIPNEPFPFVNNSEEFIDKIKSDITKTLVKFGSITVLLTFGILHLFRY
VRP*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000026gut
PLANA:0000034epidermis
PLANA:0000240body wall musculature
PLANA:0002032epidermal cell
PLANA:0002075pigment cell
Vocabulary: INTERPRO
TermDefinition
IPR040632Sulfotransfer_4
IPR027417P-loop_NTPase
Vocabulary: cellular component
TermDefinition
GO:0016020membrane
GO:0016021integral component of membrane
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR040632Sulfotransferase, S. mansonii-typePFAMPF17784Sulfotransfer_4coord: 1..206
e-value: 4.3E-59
score: 199.9
NoneNo IPR availableGENE3DG3DSA:3.40.50.300coord: 1..223
e-value: 1.5E-64
score: 220.0
NoneNo IPR availablePANTHERPTHR36978FAMILY NOT NAMEDcoord: 1..221
NoneNo IPR availableTMHMMTMhelixcoord: 217..236
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILYSSF52540P-loop containing nucleoside triphosphate hydrolasescoord: 1..218