Exonuclease 3'-5' domain-containing protein 1

Overview
NameExonuclease 3'-5' domain-containing protein 1
Smed IDSMED30023135
Length (bp)2601
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Exonuclease 3'-5' domain-containing protein 1 (SMED30023135) t-SNE clustered cells

Violin plots show distribution of expression levels for Exonuclease 3'-5' domain-containing protein 1 (SMED30023135) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Exonuclease 3'-5' domain-containing protein 1 (SMED30023135) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Exonuclease 3'-5' domain-containing protein 1 (SMED30023135) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30023135

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 2

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
cephalic gangliaSMED30023135SMESG000053185.1 dd_Smed_v4_6723_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30023135SMESG000053185.1 dd_Smed_v4_6723_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Exonuclease 3'-5' domain-containing protein 1 vs. Ensembl Celegans
Match: egal-1 (Egalitarian protein homolog [Source:UniProtKB/Swiss-Prot;Acc:Q17902])

HSP 1 Score: 77.7962 bits (190), Expect = 1.650e-14
Identity = 92/366 (25.14%), Postives = 149/366 (40.71%), Query Frame = 1 
Query:  415 MNDPNHKAMLYFLEVLLNSDHQLTIDELVDRFYCKNFTEDMRIACGNNAHGLKLFLECYPSLFRVKGNNVSAVTIDMPNSVSLISNCSSPSLVSQSNNTTNATVSSDNTETEFTCSKISKLSLKFPQKFDFNMEIEAVKFFQSKLQKRDEKWILIKSLAGHLSQADINIREVVGPQV-EFRKFLLRHPHVFEVQGELVSLKDNFPSQWLNRKPKPINGFTNKSIRPKSLNVSSINTQREFFGATSAPTTPVAHSNGLHVLARSAPLSMSGNEYRAIMFLRKVIEK-KNGSLSLSHLFL-YLGKASETIRNIIGWTKFELQEFIRKNSVFFETTPNEMILNKKANRINVIITGTSSSCDPGTRTLTN 1503
            M +  + A+L+F++ L+  + + TI +L  +F  + F+E+MR A G    GL  FL+ +PSLF V+G+ V             I N  +  L +++N    + + S N E                         EAV FF +KL K   + + IKSL GH SQA   +R V G  + EF +FL      F V+G+ V LK+                                               P    N + +     PL+    +  A+ +L+ V+E+ ++  + L   +  +  + S TIR  +     EL +F++ N   F    N++ L K  NR+N    G+ +  D G  T  N
Sbjct:    1 MEEAKNMALLFFMDHLMQKNGRRTIHDLSCQFGARGFSEEMRNAVGTTQEGLTEFLQGHPSLFTVEGDQV-------------ILNGHN-DLNAKNNPLLQSGIRSRNYEK------------------------EAVDFFVTKLTKFGPE-LQIKSLLGHRSQAAPEVRLVSGRHLKEFCEFLQSQVDYFVVEGDRVRLKN----------------------------------------------MPEPDENAIEMDDEGRPLAGVKAKQAAVEYLKSVLEQNEDQPIPLDQFYQNFCQRFSHTIRQDVATNPKELLQFLKLNRGLFFIRSNKVSLVK--NRLNE--DGSENGSDEGEETNNN 277
[Close]
BLAST of Exonuclease 3'-5' domain-containing protein 1 vs. Ensembl Celegans
Match: egal-1 (Egalitarian protein homolog [Source:UniProtKB/Swiss-Prot;Acc:Q17902])

HSP 1 Score: 77.7962 bits (190), Expect = 1.650e-14
Identity = 92/366 (25.14%), Postives = 149/366 (40.71%), Query Frame = 1 
Query:  415 MNDPNHKAMLYFLEVLLNSDHQLTIDELVDRFYCKNFTEDMRIACGNNAHGLKLFLECYPSLFRVKGNNVSAVTIDMPNSVSLISNCSSPSLVSQSNNTTNATVSSDNTETEFTCSKISKLSLKFPQKFDFNMEIEAVKFFQSKLQKRDEKWILIKSLAGHLSQADINIREVVGPQV-EFRKFLLRHPHVFEVQGELVSLKDNFPSQWLNRKPKPINGFTNKSIRPKSLNVSSINTQREFFGATSAPTTPVAHSNGLHVLARSAPLSMSGNEYRAIMFLRKVIEK-KNGSLSLSHLFL-YLGKASETIRNIIGWTKFELQEFIRKNSVFFETTPNEMILNKKANRINVIITGTSSSCDPGTRTLTN 1503
            M +  + A+L+F++ L+  + + TI +L  +F  + F+E+MR A G    GL  FL+ +PSLF V+G+ V             I N  +  L +++N    + + S N E                         EAV FF +KL K   + + IKSL GH SQA   +R V G  + EF +FL      F V+G+ V LK+                                               P    N + +     PL+    +  A+ +L+ V+E+ ++  + L   +  +  + S TIR  +     EL +F++ N   F    N++ L K  NR+N    G+ +  D G  T  N
Sbjct:    1 MEEAKNMALLFFMDHLMQKNGRRTIHDLSCQFGARGFSEEMRNAVGTTQEGLTEFLQGHPSLFTVEGDQV-------------ILNGHN-DLNAKNNPLLQSGIRSRNYEK------------------------EAVDFFVTKLTKFGPE-LQIKSLLGHRSQAAPEVRLVSGRHLKEFCEFLQSQVDYFVVEGDRVRLKN----------------------------------------------MPEPDENAIEMDDEGRPLAGVKAKQAAVEYLKSVLEQNEDQPIPLDQFYQNFCQRFSHTIRQDVATNPKELLQFLKLNRGLFFIRSNKVSLVK--NRLNE--DGSENGSDEGEETNNN 277
[Close]
BLAST of Exonuclease 3'-5' domain-containing protein 1 vs. Ensembl Celegans
Match: egal-1 (Egalitarian protein homolog [Source:UniProtKB/Swiss-Prot;Acc:Q17902])

HSP 1 Score: 77.7962 bits (190), Expect = 1.698e-14
Identity = 92/366 (25.14%), Postives = 149/366 (40.71%), Query Frame = 1 
Query:  415 MNDPNHKAMLYFLEVLLNSDHQLTIDELVDRFYCKNFTEDMRIACGNNAHGLKLFLECYPSLFRVKGNNVSAVTIDMPNSVSLISNCSSPSLVSQSNNTTNATVSSDNTETEFTCSKISKLSLKFPQKFDFNMEIEAVKFFQSKLQKRDEKWILIKSLAGHLSQADINIREVVGPQV-EFRKFLLRHPHVFEVQGELVSLKDNFPSQWLNRKPKPINGFTNKSIRPKSLNVSSINTQREFFGATSAPTTPVAHSNGLHVLARSAPLSMSGNEYRAIMFLRKVIEK-KNGSLSLSHLFL-YLGKASETIRNIIGWTKFELQEFIRKNSVFFETTPNEMILNKKANRINVIITGTSSSCDPGTRTLTN 1503
            M +  + A+L+F++ L+  + + TI +L  +F  + F+E+MR A G    GL  FL+ +PSLF V+G+ V             I N  +  L +++N    + + S N E                         EAV FF +KL K   + + IKSL GH SQA   +R V G  + EF +FL      F V+G+ V LK+                                               P    N + +     PL+    +  A+ +L+ V+E+ ++  + L   +  +  + S TIR  +     EL +F++ N   F    N++ L K  NR+N    G+ +  D G  T  N
Sbjct:    1 MEEAKNMALLFFMDHLMQKNGRRTIHDLSCQFGARGFSEEMRNAVGTTQEGLTEFLQGHPSLFTVEGDQV-------------ILNGHN-DLNAKNNPLLQSGIRSRNYEK------------------------EAVDFFVTKLTKFGPE-LQIKSLLGHRSQAAPEVRLVSGRHLKEFCEFLQSQVDYFVVEGDRVRLKN----------------------------------------------MPEPDENAIEMDDEGRPLAGVKAKQAAVEYLKSVLEQNEDQPIPLDQFYQNFCQRFSHTIRQDVATNPKELLQFLKLNRGLFFIRSNKVSLVK--NRLNE--DGSENGSDEGEETNNN 277
[Close]
BLAST of Exonuclease 3'-5' domain-containing protein 1 vs. Ensembl Celegans
Match: egal-1 (Egalitarian protein homolog [Source:UniProtKB/Swiss-Prot;Acc:Q17902])

HSP 1 Score: 77.7962 bits (190), Expect = 1.698e-14
Identity = 92/366 (25.14%), Postives = 149/366 (40.71%), Query Frame = 1 
Query:  415 MNDPNHKAMLYFLEVLLNSDHQLTIDELVDRFYCKNFTEDMRIACGNNAHGLKLFLECYPSLFRVKGNNVSAVTIDMPNSVSLISNCSSPSLVSQSNNTTNATVSSDNTETEFTCSKISKLSLKFPQKFDFNMEIEAVKFFQSKLQKRDEKWILIKSLAGHLSQADINIREVVGPQV-EFRKFLLRHPHVFEVQGELVSLKDNFPSQWLNRKPKPINGFTNKSIRPKSLNVSSINTQREFFGATSAPTTPVAHSNGLHVLARSAPLSMSGNEYRAIMFLRKVIEK-KNGSLSLSHLFL-YLGKASETIRNIIGWTKFELQEFIRKNSVFFETTPNEMILNKKANRINVIITGTSSSCDPGTRTLTN 1503
            M +  + A+L+F++ L+  + + TI +L  +F  + F+E+MR A G    GL  FL+ +PSLF V+G+ V             I N  +  L +++N    + + S N E                         EAV FF +KL K   + + IKSL GH SQA   +R V G  + EF +FL      F V+G+ V LK+                                               P    N + +     PL+    +  A+ +L+ V+E+ ++  + L   +  +  + S TIR  +     EL +F++ N   F    N++ L K  NR+N    G+ +  D G  T  N
Sbjct:    1 MEEAKNMALLFFMDHLMQKNGRRTIHDLSCQFGARGFSEEMRNAVGTTQEGLTEFLQGHPSLFTVEGDQV-------------ILNGHN-DLNAKNNPLLQSGIRSRNYEK------------------------EAVDFFVTKLTKFGPE-LQIKSLLGHRSQAAPEVRLVSGRHLKEFCEFLQSQVDYFVVEGDRVRLKN----------------------------------------------MPEPDENAIEMDDEGRPLAGVKAKQAAVEYLKSVLEQNEDQPIPLDQFYQNFCQRFSHTIRQDVATNPKELLQFLKLNRGLFFIRSNKVSLVK--NRLNE--DGSENGSDEGEETNNN 277
[Close]
BLAST of Exonuclease 3'-5' domain-containing protein 1 vs. Ensembl Fly
Match: egl (gene:FBgn0000562 transcript:FBtr0343272)

HSP 1 Score: 65.0846 bits (157), Expect = 2.502e-10
Identity = 54/193 (27.98%), Postives = 82/193 (42.49%), Query Frame = 1 
Query:  439 MLYFLEVLLNSDHQLTIDELVDRFYCKNFTEDMRIACGNNAHGLKLFLECYPSLFRVKGNNVSAVTIDMPNSVSLISNCSSPSLVSQSNNTTNATVSSDNTETEFTCSKISKLSLKFPQKFDFNMEIEAVKFFQSK-LQKRDEKWILIKSLAGHLSQADINIREVVGPQV-EFRKFLLRHPHVFEVQGELVSL 1011
            +L+FLE LL+     T+ +L  +F  K FT++MR   G +  GLK FL  YP++F V G+ V                        Q +N  +                          K D+    EA  +F++K LQ      + ++SL GH SQA   +R + G  + EF  FL++H   F+V  + V L
Sbjct:   14 LLFFLERLLDKGEPRTVHDLSCQFGNKEFTKEMRQIAGGSQSGLKKFLAQYPAIFLVDGDYVQVNAY-------------------QHHNADDGGCGG---------------------KRDYIQ--EAKDYFKNKMLQYGAAAEVPVRSLLGHRSQASPQVRHISGQHIKEFTDFLMKHTDTFKVTDDYVML 164
[Close]
BLAST of Exonuclease 3'-5' domain-containing protein 1 vs. Ensembl Fly
Match: egl (gene:FBgn0000562 transcript:FBtr0114586)

HSP 1 Score: 65.0846 bits (157), Expect = 2.502e-10
Identity = 54/193 (27.98%), Postives = 82/193 (42.49%), Query Frame = 1 
Query:  439 MLYFLEVLLNSDHQLTIDELVDRFYCKNFTEDMRIACGNNAHGLKLFLECYPSLFRVKGNNVSAVTIDMPNSVSLISNCSSPSLVSQSNNTTNATVSSDNTETEFTCSKISKLSLKFPQKFDFNMEIEAVKFFQSK-LQKRDEKWILIKSLAGHLSQADINIREVVGPQV-EFRKFLLRHPHVFEVQGELVSL 1011
            +L+FLE LL+     T+ +L  +F  K FT++MR   G +  GLK FL  YP++F V G+ V                        Q +N  +                          K D+    EA  +F++K LQ      + ++SL GH SQA   +R + G  + EF  FL++H   F+V  + V L
Sbjct:   14 LLFFLERLLDKGEPRTVHDLSCQFGNKEFTKEMRQIAGGSQSGLKKFLAQYPAIFLVDGDYVQVNAY-------------------QHHNADDGGCGG---------------------KRDYIQ--EAKDYFKNKMLQYGAAAEVPVRSLLGHRSQASPQVRHISGQHIKEFTDFLMKHTDTFKVTDDYVML 164
[Close]
BLAST of Exonuclease 3'-5' domain-containing protein 1 vs. UniProt/SwissProt
Match: sp|Q17902|EGAL1_CAEEL (Egalitarian protein homolog OS=Caenorhabditis elegans OX=6239 GN=egal-1 PE=1 SV=2)

HSP 1 Score: 77.7962 bits (190), Expect = 2.160e-13
Identity = 92/366 (25.14%), Postives = 149/366 (40.71%), Query Frame = 1 
Query:  415 MNDPNHKAMLYFLEVLLNSDHQLTIDELVDRFYCKNFTEDMRIACGNNAHGLKLFLECYPSLFRVKGNNVSAVTIDMPNSVSLISNCSSPSLVSQSNNTTNATVSSDNTETEFTCSKISKLSLKFPQKFDFNMEIEAVKFFQSKLQKRDEKWILIKSLAGHLSQADINIREVVGPQV-EFRKFLLRHPHVFEVQGELVSLKDNFPSQWLNRKPKPINGFTNKSIRPKSLNVSSINTQREFFGATSAPTTPVAHSNGLHVLARSAPLSMSGNEYRAIMFLRKVIEK-KNGSLSLSHLFL-YLGKASETIRNIIGWTKFELQEFIRKNSVFFETTPNEMILNKKANRINVIITGTSSSCDPGTRTLTN 1503
            M +  + A+L+F++ L+  + + TI +L  +F  + F+E+MR A G    GL  FL+ +PSLF V+G+ V             I N  +  L +++N    + + S N E                         EAV FF +KL K   + + IKSL GH SQA   +R V G  + EF +FL      F V+G+ V LK+                                               P    N + +     PL+    +  A+ +L+ V+E+ ++  + L   +  +  + S TIR  +     EL +F++ N   F    N++ L K  NR+N    G+ +  D G  T  N
Sbjct:    1 MEEAKNMALLFFMDHLMQKNGRRTIHDLSCQFGARGFSEEMRNAVGTTQEGLTEFLQGHPSLFTVEGDQV-------------ILNGHN-DLNAKNNPLLQSGIRSRNYEK------------------------EAVDFFVTKLTKFGPE-LQIKSLLGHRSQAAPEVRLVSGRHLKEFCEFLQSQVDYFVVEGDRVRLKN----------------------------------------------MPEPDENAIEMDDEGRPLAGVKAKQAAVEYLKSVLEQNEDQPIPLDQFYQNFCQRFSHTIRQDVATNPKELLQFLKLNRGLFFIRSNKVSLVK--NRLNE--DGSENGSDEGEETNNN 277
[Close]
BLAST of Exonuclease 3'-5' domain-containing protein 1 vs. TrEMBL
Match: A0A267EF48 (Uncharacterized protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig015220g2 PE=4 SV=1)

HSP 1 Score: 438.343 bits (1126), Expect = 5.578e-139
Identity = 288/756 (38.10%), Postives = 380/756 (50.26%), Query Frame = 1 
Query:  367 SCLGYLKKREVFLYKMMNDPNHKAMLYFLEVLLNSDH--QLTIDELVDRFYCKNFTEDMRIACGNNAHGLKLFLECYPSLFRVKGNNVSAVTIDMPNSVSLISN-------------------------------CSSPSLVSQSNNTTNATVSSDNTETEFTCSKISKLSLKFPQKFDFNMEIEAVKFFQSKLQKRDEKWILIKSLAGHLSQADINIREVVGPQVEFRKFLLRHPHVFEVQGELVSLKDNFPSQWL-------------------------NRKPKPINGFTNKS-----IRPKSLNVSSINTQREFFGATSAPTTPVAHSNGLHVLARSA----------PLSMSGNEYRAIMFLRKVIEKKNGSLSLSHLFLYL---GKASETIRNIIGWTKFELQEFIRKNSVFFETTPNEMILN-KKANRINVIITGTSSSC----------------DPGTRTLTNRCGNVFHVAKLWGIIDLGKHEHVFFDKSIFRHLDDLQRHFKIGETLYFNAILAPKESRAKWRATQVWKESDREMVEKFSGNILSRTTTNSGINNDCLSDANSIITDASDDLTHFANGEPNNNTKMLPEDVEEELTRILKLTKTDCENDEDYFANFDKFYKKDVLIEFNKPFKNDAGKLCDLDYDDDSQSIESIPTPDFSSQTSVQSEXXXXXXXXXXXXXXXDHYAILQSTPSNKKSVFIKIVTKIPEEPKLLQPDSTKSPKMVSIATQTLSTGDIMATNLFHE 2355
            SCL YLK REVFLYKMM DP H++MLYFLEVL+N D    + +  L   F+ ++FT+DMR A  + A GLK FL+ YPSLFRV G +VSAV++D   ++S IS                                 SS S  SQ +N         N          + L        DF  E EAV+FFQ++L +R+++W+ IKSLAGHLSQA   IR VVGPQ EF +FL RH HVFE+QGELV L+D F +  L                         NR    + G  + S      RPKSL + S +  +   G  S PTTP + +      +             P+SM+  EY+ +MFLRKV+E+K G LS+  LF  L   G+ +E  RN+IGWT+ EL+EF+RK S+FF+  P +  ++ ++A ++N+IITG   S                     RTLTNR G VFH A+LWGIIDLG+HEHVFFDKSIF+H+DDLQ+HFK+ E LYFNAILAPKESRAKWRATQVWKE+DR ++E+ SG  L  T   S +        + +  +  D L      E  ++ K L +  E EL R+L+                                            D DS++  S+P+P  +   S   +                H    Q TPS   S       + P  P           KMV++ATQTLSTG+IMAT +FH+
Sbjct:    3 SCLSYLKSREVFLYKMMGDPAHRSMLYFLEVLMNVDSPDGVRLTSLSQHFHSRHFTQDMREAVAS-AGGLKPFLQKYPSLFRVNGESVSAVSLD--GALSQISGDTAATAASSSSSSSTMAAAAAQAAAAAAAAASSSGSGRSQHHNGGRRDSIGSNNSARSGSDAAAALHSVSGDPNDFARETEAVRFFQTRLSRREDRWVPIKSLAGHLSQAPPEIRAVVGPQAEFARFLARHRHVFELQGELVGLRDTFAAHCLSGGGVGGSGAGGSSQRHQQQQLQSRNRGSGNLGGGLHHSQSARMARPKSLVMPSCSGDQRSSG-KSLPTTPTSTAGPGGGSSSGGLSSSISGGAGPVSMTAAEYKTVMFLRKVLERKGGLLSIPQLFSVLSRSGEGAEQARNLIGWTQIELEEFLRKQSLFFDIHPADGSVSCRRAQKLNIIITGGRRSNRDGKSGAGGGGSAGVDQTAPRTLTNRRGRVFHCARLWGIIDLGRHEHVFFDKSIFKHVDDLQKHFKMNEILYFNAILAPKESRAKWRATQVWKEADRAVMERLSGGAL--TAAASAVK-------SPLGPEPLDSL------ELADDIKFLSDIDETELRRVLQ-------------------------------------------DDADSRTSSSVPSPVLTPDLSEGKDEPPPPTL---------HQQPPQPTPSGSTSGGCCSRCQAPTTPAAC----NGGRKMVTMATQTLSTGEIMATQIFHD 683
[Close]
BLAST of Exonuclease 3'-5' domain-containing protein 1 vs. TrEMBL
Match: A0A267E585 (Uncharacterized protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig021118g1 PE=4 SV=1)

HSP 1 Score: 424.091 bits (1089), Expect = 1.647e-133
Identity = 244/553 (44.12%), Postives = 315/553 (56.96%), Query Frame = 1 
Query:  367 SCLGYLKKREVFLYKMMNDPNHKAMLYFLEVLLNSDH--QLTIDELVDRFYCKNFTEDMRIACGNNAHGLKLFLECYPSLFRVKGNNVSAVTIDMPNSVSLISN------------------------------CSSPSLVSQSNNTTNATVSSDNTETEFTCSKISKLSLKFPQKFDFNMEIEAVKFFQSKLQKRDEKWILIKSLAGHLSQADINIREVVGPQVEFRKFLLRHPHVFEVQGELVSLKDNFPSQWL-------------------------NRKPKPINGFTNKS-----IRPKSLNVSSINTQREFFGATSAPTTPVAHSNGLHVLARSA---------PLSMSGNEYRAIMFLRKVIEKKNGSLSLSHLFLYL---GKASETIRNIIGWTKFELQEFIRKNSVFFETTPNEMILN-KKANRINVIITGTSSSC---------------DPGTRTLTNRCGNVFHVAKLWGIIDLGKHEHVFFDKSIFRHLDDLQRHFKIGETLYFNAILAPKESRAKWRATQVWKESDREMVEKFSGNILS 1755
            SCL YLK REVFLYKMM DP H++MLYFLEVL+N D    + +  L   F+ ++FT+DMR A  + A GLK FL+ YPSLFRV G +VSAV++D   ++S IS                                SS S  SQ +N         N          + L        DF  E EAV+FFQ++L +R+++W+ IKSLAGHLSQA   IR VVGPQ EF +FL RH HVFE+QGELV L+D F +  L                         NR    + G  + S      RPKSL + S +  +   G  S PTTP + +                   P+SM+  EY+ +MFLRKV+E+K G LS+  LF  L   G+ +E  RN+IGWT+ EL+EF+RK S+FF+  P +  ++ ++A ++N+IITG   S                    RTLTNR G VFH A+LWGIIDLG+HEHVFFDKSIF+H+DDLQ+HFK+ E LYFNAILAPKESRAKWRATQVWKE+DR ++E+ SG  L+
Sbjct:    3 SCLSYLKSREVFLYKMMGDPAHRSMLYFLEVLMNVDSPDGVRLTSLSQHFHSRHFTQDMREAVAS-AGGLKPFLQKYPSLFRVNGESVSAVSLD--GALSQISGDTAATAASSSSSSSTMAAAAAQAAAAAAAASSSGSGRSQHHNGGRRDSIGSNNSARSGSDAAAALHSVSGDPNDFARETEAVRFFQTRLSRREDRWVPIKSLAGHLSQAPPEIRAVVGPQAEFARFLARHRHVFELQGELVGLRDTFAAHCLSGGGGGGSGGGGSSQRHQQQQLQSRNRGSGNLGGGLHHSQSARMARPKSLVMPSCSGDQRSSG-KSLPTTPTSTAGPGGSSGGGLSSSISGGAGPVSMTAAEYKTVMFLRKVLERKGGLLSIPQLFSVLSRSGEGAEQARNLIGWTQIELEEFLRKQSLFFDIHPADGSVSCRRAQKLNIIITGGRRSNRDGKSGAGGGSAGVDQTAPRTLTNRRGRVFHCARLWGIIDLGRHEHVFFDKSIFKHVDDLQKHFKMNEILYFNAILAPKESRAKWRATQVWKEADRAVMERLSGGALT 551
[Close]
BLAST of Exonuclease 3'-5' domain-containing protein 1 vs. TrEMBL
Match: A0A1I8HNR9 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 395.971 bits (1016), Expect = 2.429e-114
Identity = 230/531 (43.31%), Postives = 297/531 (55.93%), Query Frame = 1 
Query:  340 DSQNYEDLDSCLGYLKKREVFLYKMMNDPNHKAMLYFLEVLLNSDHQ--LTIDELVDRFYCKNFTEDMRIACGNNAHGLKLFLECYPSLFRVKGNNVSAVTIDMPNSVSLISN-------------------------------CSSPSLVSQSNNTTNATVSSDNTETEFTCSKISKLSLKFPQKFDFNMEIEAVKFFQSKLQKRDEKWILIKSLAGHLSQADINIREVVGPQVEFRKFLLRHPHVFEVQGELVSLKDNFPSQWLNRKPKPINGFTNKSIRPKSLNVSSINTQREFFGATSAPTTPVAHSNGLHVLARSAP------LSMSGNEYRAIMFLRKVIEKKNGSLSLSHLFLYL---GKASETIRNIIGWTKFELQEFIRKNSVFFETTPNEMILN-KKANRINVIITGTSSSC----------------DPGTRTLTNRCGNVFHVAKLWGIIDLGKHEHVFFDKSIFRHLDDLQRHFKIGETLYFNAILAPKESRAKWRATQVWKESDREMVEKFSGNILS 1755
            D  +  D  SCL YLK REVFLYKMM DP H++MLYFLEVL+N D    + +  L   F+ ++FT+DMR A  + A GLK FL+ YPSLFRV G +VSAV++D   ++S IS                                 SS S  SQ +N         N          + L        DF  E EAV+FFQ++L +R+++W+ IKSLAGHLSQA   IR VVGPQ EF +FL RH HVFE+QGEL                                               S PTTP + +      + S+       +SM+  EY+ +MFLRKV+E+K G LS+  LF  L   G+ +E  RN+IGWT+ EL+EF+RK S+FF+  P +  ++ ++A ++N+IITG   S                     RTLTNR G VFH A+LWGIIDLG+HEHVFFDKSIF+H+DDLQ+HFK+ E LYFNAILAPKESRAKWRATQVWKE+DR ++E+ SG  L+
Sbjct:  765 DPTDVTDAQSCLSYLKSREVFLYKMMGDPAHRSMLYFLEVLMNVDSPDGVRLTSLSQHFHSRHFTQDMREAVAS-AGGLKPFLQKYPSLFRVNGESVSAVSLD--GALSQISGDTAATAASSSSSSSTMAAAAAQAAAAAAAAASSSGSGRSQHHNGGRRDSIGSNNSARSGSDAAAALHSVSGDPNDFARETEAVRFFQTRLSRREDRWVPIKSLAGHLSQAPPEIRAVVGPQAEFARFLARHRHVFELQGEL-----------------------------------------------SLPTTPTSTAGPGGGSSSSSISGGAGPVSMTAAEYKTVMFLRKVLERKGGLLSIPQLFSVLSRSGEGAEQARNLIGWTQIELEEFLRKQSLFFDIHPADGSVSCRRAQKLNIIITGGRRSNRDGKSGAGGGGSAGVDQTAPRTLTNRRGRVFHCARLWGIIDLGRHEHVFFDKSIFKHVDDLQKHFKMNEILYFNAILAPKESRAKWRATQVWKEADRAVMERLSGGALT 1245
[Close]
BLAST of Exonuclease 3'-5' domain-containing protein 1 vs. TrEMBL
Match: A0A210Q935 (Uncharacterized protein OS=Mizuhopecten yessoensis OX=6573 GN=KP79_PYT11276 PE=4 SV=1)

HSP 1 Score: 345.895 bits (886), Expect = 1.083e-104
Identity = 196/461 (42.52%), Postives = 269/461 (58.35%), Query Frame = 1 
Query:  412 MMNDPNHKAMLYFLEVLLNSDHQLTIDELVDRFYCKNFTEDMRIACGNNAHGLKLFLECYPSLFRVKGNNVSAVTIDMPNSVSLISNCSSPSLVSQSNNTTNATVSSDNTETEFTCSKISKLSLKFPQKFDFNMEIEAVKFFQSKLQKRDEKWILIKSLAGHLSQADINIREVVGPQVEFRKFLLRHPHVFEVQGELVSLKDNFPSQWLNRKPKPIN------------GFTNKSIRPKSLNVSSINTQR--EFFGATSAPTTPVAHSNGLHVLA-RSAPLSMSGNEYRAIMFLRKVIEKKNGSLSLSHLFLYLGKASETIRNIIGWTKFELQEFIRKNSVFFETTPNEMILNKKANRINVIITGTSSSCDPGTRTLTNRCGNVFHVAKLWGIIDLGKHEHVFFDKSIF-RHLDDLQRHFKIGETLYFNAILAPKESRAKWRATQVWK--ESDREMVEKFS 1740
            M +DP HKAMLYFLE+L++S+  LTI +L  RF  +NFT +MR + G N  GLK FL+ YPSLF V GN                       L+S ++ T N   +   +    T S         P   + + E EAV+FF++KL KR EKW+ IKSLAGHLSQA + IRE VGPQ EFR++LLRHP ++E++G+   LKDN     +N   +P+              +  K + P  L        R  E F   S   +P +    L   + RS P +M+ NEY+A+MF++ +IEKK G + L  L  + G+A E++RN +GW+K EL  F+  +S  F    NEM+   K+ R+NVIITG+     P   T+TNR G ++HVAKLWGIIDLGKHEHVF D+SIF +H+DDL +   +GE   F+AI A K SRA+WRAT++WK  ES  E++E+ +
Sbjct:    1 MADDPGHKAMLYFLEILMHSNAPLTISQLAGRFGSRNFTAEMRQSAGGNETGLKKFLQKYPSLFSVNGN-----------------------LISLNDGTANLGRNFRESSPASTSSG--------PSAPEVSTETEAVQFFRAKLLKRGEKWMPIKSLAGHLSQAPLEIRECVGPQNEFREWLLRHPLIYELKGDNACLKDNITYSAVNPNSEPLEDMNFNNRYSANLSYLEKDMFPSQLTPKLKRRPRSLEMFEPNSPLMSPRSPMKQLPTPSPRSGPTTMTANEYKAVMFIKGIIEKK-GDMKLGSLVNHFGQAPESLRNTVGWSKQELDHFLVDHSNIFIIGENEMVSVIKSARLNVIITGSRPPASPKP-TVTNRKGRIYHVAKLWGIIDLGKHEHVFIDRSIFGKHVDDLHKILCVGEMACFDAIPAAKGSRARWRATKIWKEHESIEELIERVA 428
[Close]
BLAST of Exonuclease 3'-5' domain-containing protein 1 vs. TrEMBL
Match: K1R391 (Uncharacterized protein OS=Crassostrea gigas OX=29159 GN=CGI_10015984 PE=4 SV=1)

HSP 1 Score: 338.961 bits (868), Expect = 3.746e-102
Identity = 193/461 (41.87%), Postives = 269/461 (58.35%), Query Frame = 1 
Query:  412 MMNDPNHKAMLYFLEVLLNSDHQLTIDELVDRFYCKNFTEDMRIACGNNAHGLKLFLECYPSLFRVKGNNVSAVTIDMPNSVSLISNCSSPSLVSQSNNTTNATVSSDNTETEFTCSKISKLSLKFPQKFDFNMEIEAVKFFQSKLQKRDEKWILIKSLAGHLSQADINIREVVGPQVEFRKFLLRHPHVFEVQGELVSLKDNFPSQWLNRKPKPINGFTNKS------------IRPKSLNVSSINTQREFFGATSAPTTPVAHSNGLHVLA---RSAPLSMSGNEYRAIMFLRKVIEKKNGSLSLSHLFLYLGKASETIRNIIGWTKFELQEFIRKNSVFFETTPNEMILNKKANRINVIITGTSSSCDPGTRTLTNRCGNVFHVAKLWGIIDLGKHEHVFFDKSIF-RHLDDLQRHFKIGETLYFNAILAPKESRAKWRATQVWKESD--REMVEKFS 1740
            M +D  HKAMLYFLE+L+NS   LTI +L  RF  +NFT +MR A G N  GLK FL+ YPSLF V GN V                C +    S  +N   ++ +S               S   P+    ++E EAV++F+ K+ K+ EKW+ I SLAGHLSQA + IRE VGPQ EFR +LLRHP +FE++GE VSLKDN     +N   + I     KS            I PKS          E      +P +P+ + N L   +   RS P +M+ NEY+A+MF++ +IEKK G + L+ L  +  +A E++RN IGWTK +L +F+  +   F  T +E++   K  R+NVIITG+     P    +T R G ++H+AKLWGIIDLG+HEHVF D++IF +H+DDL +   +GE + F+AI APK SRA+WRAT+VWKE++   +++EK +
Sbjct:    1 MADDAGHKAMLYFLEILMNSPAPLTISQLAGRFGSRNFTSEMRQAAGGNETGLKKFLQKYPSLFTVNGNLV----------------CLNDGTASLGSNFRESSPAS---------------STGLPE---VSVETEAVQYFRGKMMKKGEKWMPINSLAGHLSQASVEIRECVGPQSEFRDWLLRHPLIFEIEGENVSLKDNITYSAINPNAEAIEDVELKSRFNQDKEQFVPPITPKSKRRPKSLDMLEPRSPLMSPRSPMVNKN-LPTPSPNPRSGPATMTANEYKAVMFIKSIIEKK-GDMKLAALTGHFSQAPESLRNTIGWTKSDLSKFLADHGNIFTITEDEVVSVIKNARLNVIITGSRPQVSPKP-VVTARKGRIYHIAKLWGIIDLGRHEHVFIDRNIFGKHIDDLNKVLTVGEMVCFDAIPAPKGSRARWRATKVWKENESIEDLIEKVA 424
[Close]
BLAST of Exonuclease 3'-5' domain-containing protein 1 vs. Planmine SMEST
Match: SMESG000053185.1 (SMESG000053185.1)

HSP 1 Score: 1384.39 bits (3582), Expect = 0.000e+0
Identity = 685/688 (99.56%), Postives = 686/688 (99.71%), Query Frame = 1 
Query:  310 MSEISDSDLYDSQNYEDLDSCLGYLKKREVFLYKMMNDPNHKAMLYFLEVLLNSDHQLTIDELVDRFYCKNFTEDMRIACGNNAHGLKLFLECYPSLFRVKGNNVSAVTIDMPNSVSLISNCSSPSLVSQSNNTTNATVSSDNTETEFTCSKISKLSLKFPQKFDFNMEIEAVKFFQSKLQKRDEKWILIKSLAGHLSQADINIREVVGPQVEFRKFLLRHPHVFEVQGELVSLKDNFPSQWLNRKPKPINGFTNKSIRPKSLNVSSINTQREFFGATSAPTTPVAHSNGLHVLARSAPLSMSGNEYRAIMFLRKVIEKKNGSLSLSHLFLYLGKASETIRNIIGWTKFELQEFIRKNSVFFETTPNEMILNKKANRINVIITGTSSSCDPGTRTLTNRCGNVFHVAKLWGIIDLGKHEHVFFDKSIFRHLDDLQRHFKIGETLYFNAILAPKESRAKWRATQVWKESDREMVEKFSGNILSRTTTNSGINNDCLSDANSIITDASDDLTHFANGEPNNNTKMLPEDVEEELTRILKLTKTDCENDEDYFANFDKFYKKDVLIEFNKPFKNDAGKLCDLDYDDDSQSIESIPTPDFSSQTSVQSEXXXXXXXXXXXXXXXDHYAILQSTPSNKKSVFIKIVTKIPEEPKLLQPDSTKSPKMVSIATQTLSTGDIMATNLFHEGISQIV 2373
            MSEISDSDLYDSQNYEDLDSCLGYLKKREVFLYKMMNDPNHKAMLYFLEVLLNSDHQLTIDELVDRFYCKNFTEDMRIACGNNAHGLKLFLECYPSLFRVKGNNVSAVTIDMPNSVSLISNCSSPSLVSQSNNTTNATVSSDNTETEFTCSKISKLSLKFPQKFDFNMEIEAVKFFQSKLQKRDEKWILIKSLAGHLSQADINIREVVGPQVEFRKFLLRHPHVFEVQGELVSLKDNFPSQWLNRKPKPINGFTNKSIRPKSLNVSSINTQREFF ATSAPTTPVAHSNGLHVLARSAPLSMSGNEYRAIMFLRKVIEKKNGSLSLSHLFLYLGKASETIRNIIGWTKFELQEFIRKNSVFFETTPNEMILNKKANRINVIITGTSSSCDPGTRTLTNRCGNVFHVAKLWGIIDLGKHEHVFFDKSIFRHLDDLQRHFKIGETLYFNAILAPKESRAKWRATQVWKESDREMVEKFSGNILSRTTTNSGINNDCLSDANSIITDASDDLTHF+N EPNNNTKMLPEDVEEELTRILKLTKTDCENDEDYFANFDKFYKKDVLIEFNKPFKNDAGKLCDLDYDDDSQSIESIPTPDFSSQTSVQSEPSVASHPLSAPAASSDHYAILQSTPSNKKSVFIKIVTKIPEEPKLLQPDSTKSPKMVSIATQTLSTGDIMATNLFHEGISQIV
Sbjct:    1 MSEISDSDLYDSQNYEDLDSCLGYLKKREVFLYKMMNDPNHKAMLYFLEVLLNSDHQLTIDELVDRFYCKNFTEDMRIACGNNAHGLKLFLECYPSLFRVKGNNVSAVTIDMPNSVSLISNCSSPSLVSQSNNTTNATVSSDNTETEFTCSKISKLSLKFPQKFDFNMEIEAVKFFQSKLQKRDEKWILIKSLAGHLSQADINIREVVGPQVEFRKFLLRHPHVFEVQGELVSLKDNFPSQWLNRKPKPINGFTNKSIRPKSLNVSSINTQREFFSATSAPTTPVAHSNGLHVLARSAPLSMSGNEYRAIMFLRKVIEKKNGSLSLSHLFLYLGKASETIRNIIGWTKFELQEFIRKNSVFFETTPNEMILNKKANRINVIITGTSSSCDPGTRTLTNRCGNVFHVAKLWGIIDLGKHEHVFFDKSIFRHLDDLQRHFKIGETLYFNAILAPKESRAKWRATQVWKESDREMVEKFSGNILSRTTTNSGINNDCLSDANSIITDASDDLTHFSNSEPNNNTKMLPEDVEEELTRILKLTKTDCENDEDYFANFDKFYKKDVLIEFNKPFKNDAGKLCDLDYDDDSQSIESIPTPDFSSQTSVQSEPSVASHPLSAPAASSDHYAILQSTPSNKKSVFIKIVTKIPEEPKLLQPDSTKSPKMVSIATQTLSTGDIMATNLFHEGISQIV 688
[Close]
BLAST of Exonuclease 3'-5' domain-containing protein 1 vs. Planmine SMEST
Match: SMESG000053185.1 (SMESG000053185.1)

HSP 1 Score: 1185.63 bits (3066), Expect = 0.000e+0
Identity = 570/573 (99.48%), Postives = 571/573 (99.65%), Query Frame = 1 
Query:  310 MSEISDSDLYDSQNYEDLDSCLGYLKKREVFLYKMMNDPNHKAMLYFLEVLLNSDHQLTIDELVDRFYCKNFTEDMRIACGNNAHGLKLFLECYPSLFRVKGNNVSAVTIDMPNSVSLISNCSSPSLVSQSNNTTNATVSSDNTETEFTCSKISKLSLKFPQKFDFNMEIEAVKFFQSKLQKRDEKWILIKSLAGHLSQADINIREVVGPQVEFRKFLLRHPHVFEVQGELVSLKDNFPSQWLNRKPKPINGFTNKSIRPKSLNVSSINTQREFFGATSAPTTPVAHSNGLHVLARSAPLSMSGNEYRAIMFLRKVIEKKNGSLSLSHLFLYLGKASETIRNIIGWTKFELQEFIRKNSVFFETTPNEMILNKKANRINVIITGTSSSCDPGTRTLTNRCGNVFHVAKLWGIIDLGKHEHVFFDKSIFRHLDDLQRHFKIGETLYFNAILAPKESRAKWRATQVWKESDREMVEKFSGNILSRTTTNSGINNDCLSDANSIITDASDDLTHFANGEPNNNTKMLPEDVEEELTRILKLTKTDCENDEDYFANFDKFYKKDVLIEFNKPFKNDA 2028
            MSEISDSDLYDSQNYEDLDSCLGYLKKREVFLYKMMNDPNHKAMLYFLEVLLNSDHQLTIDELVDRFYCKNFTEDMRIACGNNAHGLKLFLECYPSLFRVKGNNVSAVTIDMPNSVSLISNCSSPSLVSQSNNTTNATVSSDNTETEFTCSKISKLSLKFPQKFDFNMEIEAVKFFQSKLQKRDEKWILIKSLAGHLSQADINIREVVGPQVEFRKFLLRHPHVFEVQGELVSLKDNFPSQWLNRKPKPINGFTNKSIRPKSLNVSSINTQREFF ATSAPTTPVAHSNGLHVLARSAPLSMSGNEYRAIMFLRKVIEKKNGSLSLSHLFLYLGKASETIRNIIGWTKFELQEFIRKNSVFFETTPNEMILNKKANRINVIITGTSSSCDPGTRTLTNRCGNVFHVAKLWGIIDLGKHEHVFFDKSIFRHLDDLQRHFKIGETLYFNAILAPKESRAKWRATQVWKESDREMVEKFSGNILSRTTTNSGINNDCLSDANSIITDASDDLTHF+N EPNNNTKMLPEDVEEELTRILKLTKTDCENDEDYFANFDKFYKKDVLIEFNKPFKNDA
Sbjct:    1 MSEISDSDLYDSQNYEDLDSCLGYLKKREVFLYKMMNDPNHKAMLYFLEVLLNSDHQLTIDELVDRFYCKNFTEDMRIACGNNAHGLKLFLECYPSLFRVKGNNVSAVTIDMPNSVSLISNCSSPSLVSQSNNTTNATVSSDNTETEFTCSKISKLSLKFPQKFDFNMEIEAVKFFQSKLQKRDEKWILIKSLAGHLSQADINIREVVGPQVEFRKFLLRHPHVFEVQGELVSLKDNFPSQWLNRKPKPINGFTNKSIRPKSLNVSSINTQREFFSATSAPTTPVAHSNGLHVLARSAPLSMSGNEYRAIMFLRKVIEKKNGSLSLSHLFLYLGKASETIRNIIGWTKFELQEFIRKNSVFFETTPNEMILNKKANRINVIITGTSSSCDPGTRTLTNRCGNVFHVAKLWGIIDLGKHEHVFFDKSIFRHLDDLQRHFKIGETLYFNAILAPKESRAKWRATQVWKESDREMVEKFSGNILSRTTTNSGINNDCLSDANSIITDASDDLTHFSNSEPNNNTKMLPEDVEEELTRILKLTKTDCENDEDYFANFDKFYKKDVLIEFNKPFKNDA 573

HSP 2 Score: 145.976 bits (367), Expect = 4.206e-36
Identity = 71/72 (98.61%), Postives = 72/72 (100.00%), Query Frame = 2 
Query: 2078 VFRLRISVPKQASNQNLALRLIRYQLQQLHRIIMLFFNQLHQTKNQFSSKLLQKFPKNLSYFNLIVRRVPKW 2293
            VFRLRISVPKQASNQNLALRLIRYQLQQLHRIIMLFFNQLHQTKNQFSSKLL+KFPKNLSYFNLIVRRVPKW
Sbjct:  574 VFRLRISVPKQASNQNLALRLIRYQLQQLHRIIMLFFNQLHQTKNQFSSKLLRKFPKNLSYFNLIVRRVPKW 645
[Close]
BLAST of Exonuclease 3'-5' domain-containing protein 1 vs. Planmine SMEST
Match: SMESG000027548.1 (SMESG000027548.1)

HSP 1 Score: 442.195 bits (1136), Expect = 1.762e-145
Identity = 235/527 (44.59%), Postives = 338/527 (64.14%), Query Frame = 1 
Query:  373 LGYLKKREVFLYKMMNDPNHKAMLYFLEVLLNSDHQLTIDELVDRFYCKNFTEDMRIACGNNAHGLKLFLECYPSLFRVKGNNVSAVTIDMPNSVSLISNCSSPSLVSQSNNTTNATVSSDNTETEFTCSKISKLSLKFPQKFDFNMEIEAVKFFQSKLQKRDEKWILIKSLAGHLSQADINIREVVGPQVEFRKFLLRHPHVFEVQGELVSLKDNFPSQWLNR--KPKPINGFTN-KSIRPKSLNVSSINTQREFFGATSAPTTPVAHSNGLHVLARSAPLSMSGNEYRAIMFLRKVIEKK-NGSLSLSHLFLYLGKASETIRNIIGWTKFELQEFIRKNSVFFETTPNEMILNKKANRINVIITGTSSSCDPGTRTLTNRCGNVFHVAKLWGIIDLGKHEHVFFDKSIFRHLDDLQRHFKIGETLYFNAILAPKESRAKWRATQVWKESDREMVEKFSGNIL---SRTTTNS-GINNDCLSDANSIITDASDDLTHFANGEPNNNTKMLPEDVEEELTRILKLTK 1929
            + YL  RE  LYKMMND +H+AML+ LEVL+NSD  + ++EL ++ +C   ++DM+  CGN+  GLKLF+E YPSLF++ GN +S + +         S+C+S             ++ +DN  +    S++ +L  + P   D   E EAVK+FQS+L +R++ W+ IKSLAGHLSQA  +IR+VVGPQ+ FRKFL RHPHVFE+QG+LVSL++NF S  L++  + +P +   N K I  K   VS I  +           T +   + L+       +++   +++A MFLR VI KK N  +S+SHLFLYL +A  ++R  IGWTK EL+EFIRK S  FE      +       I++I+    +S D G RT+ N+ G V+H+ KLWGI+DLG+HEHVFFDKSIF+HLDDLQRHF+IGE +YFNAILAPK+SRAKWRATQVWKESD++MV++F G+++    +++TN+  I++D LS   SI+++    + ++              D++EEL  IL+L+K
Sbjct:    1 MNYL--REASLYKMMNDSHHRAMLFLLEVLMNSDTTVALEELGEKIFCDKVSKDMQDVCGNDLEGLKLFMEKYPSLFQIDGNQISPIDV---------SDCAS-------------SIEADNGHSSIHDSELYRL--RHP--VDLTQETEAVKYFQSQLTRREDHWVSIKSLAGHLSQASESIRKVVGPQINFRKFLQRHPHVFEIQGDLVSLRNNFSSICLSKGSRNRPRSAIINSKKIMKKGRPVSMIVNK-----------TEIEKESKLYPPTSKGSVAIDSRDFQAAMFLRTVIRKKSNKKISISHLFLYLAQAPSSVRESIGWTKIELEEFIRKQSTLFEIDKKGQVTCLDTEEISIIVNDDLTSKD-GVRTIINQRGKVYHITKLWGIVDLGQHEHVFFDKSIFKHLDDLQRHFQIGEYIYFNAILAPKQSRAKWRATQVWKESDKDMVQRFCGDLIYGTKKSSTNTLNIDSDSLSCCRSIMSEQRHIIENY-------------HDIDEELAEILQLSK 474
[Close]
BLAST of Exonuclease 3'-5' domain-containing protein 1 vs. Planmine SMEST
Match: SMESG000027548.1 (SMESG000027548.1)

HSP 1 Score: 432.565 bits (1111), Expect = 5.867e-142
Identity = 228/514 (44.36%), Postives = 330/514 (64.20%), Query Frame = 1 
Query:  412 MMNDPNHKAMLYFLEVLLNSDHQLTIDELVDRFYCKNFTEDMRIACGNNAHGLKLFLECYPSLFRVKGNNVSAVTIDMPNSVSLISNCSSPSLVSQSNNTTNATVSSDNTETEFTCSKISKLSLKFPQKFDFNMEIEAVKFFQSKLQKRDEKWILIKSLAGHLSQADINIREVVGPQVEFRKFLLRHPHVFEVQGELVSLKDNFPSQWLNR--KPKPINGFTN-KSIRPKSLNVSSINTQREFFGATSAPTTPVAHSNGLHVLARSAPLSMSGNEYRAIMFLRKVIEKK-NGSLSLSHLFLYLGKASETIRNIIGWTKFELQEFIRKNSVFFETTPNEMILNKKANRINVIITGTSSSCDPGTRTLTNRCGNVFHVAKLWGIIDLGKHEHVFFDKSIFRHLDDLQRHFKIGETLYFNAILAPKESRAKWRATQVWKESDREMVEKFSGNIL---SRTTTNS-GINNDCLSDANSIITDASDDLTHFANGEPNNNTKMLPEDVEEELTRILKLTK 1929
            MMND +H+AML+ LEVL+NSD  + ++EL ++ +C   ++DM+  CGN+  GLKLF+E YPSLF++ GN +S + +         S+C+S             ++ +DN  +    S++ +L  + P   D   E EAVK+FQS+L +R++ W+ IKSLAGHLSQA  +IR+VVGPQ+ FRKFL RHPHVFE+QG+LVSL++NF S  L++  + +P +   N K I  K   VS I  +           T +   + L+       +++   +++A MFLR VI KK N  +S+SHLFLYL +A  ++R  IGWTK EL+EFIRK S  FE      +       I++I+    +S D G RT+ N+ G V+H+ KLWGI+DLG+HEHVFFDKSIF+HLDDLQRHF+IGE +YFNAILAPK+SRAKWRATQVWKESD++MV++F G+++    +++TN+  I++D LS   SI+++    + ++              D++EEL  IL+L+K
Sbjct:    1 MMNDSHHRAMLFLLEVLMNSDTTVALEELGEKIFCDKVSKDMQDVCGNDLEGLKLFMEKYPSLFQIDGNQISPIDV---------SDCAS-------------SIEADNGHSSIHDSELYRL--RHP--VDLTQETEAVKYFQSQLTRREDHWVSIKSLAGHLSQASESIRKVVGPQINFRKFLQRHPHVFEIQGDLVSLRNNFSSICLSKGSRNRPRSAIINSKKIMKKGRPVSMIVNK-----------TEIEKESKLYPPTSKGSVAIDSRDFQAAMFLRTVIRKKSNKKISISHLFLYLAQAPSSVRESIGWTKIELEEFIRKQSTLFEIDKKGQVTCLDTEEISIIVNDDLTSKD-GVRTIINQRGKVYHITKLWGIVDLGQHEHVFFDKSIFKHLDDLQRHFQIGEYIYFNAILAPKQSRAKWRATQVWKESDKDMVQRFCGDLIYGTKKSSTNTLNIDSDSLSCCRSIMSEQRHIIENY-------------HDIDEELAEILQLSK 463
[Close]
BLAST of Exonuclease 3'-5' domain-containing protein 1 vs. Planmine SMEST
Match: SMESG000028883.1 (SMESG000028883.1)

HSP 1 Score: 260.381 bits (664), Expect = 1.521e-76
Identity = 179/484 (36.98%), Postives = 282/484 (58.26%), Query Frame = 1 
Query:  415 MNDPNHKAMLYFLEVLLNSDHQLTIDELVDRFYCKNFTEDMRIACGNNAHGLKLFLECYPSLFRVKGNNVSAVTIDMPNSVSLISNCSSPSLVSQSNNTTNATVSSDNTETEFTCSKISKLSLKFPQKFDFNMEIEAVKFFQSKLQKRDEKWILIKSLAGHLSQADINIREVVGPQVEFRKFLLRHPHVFEVQGELVSLKDNFPSQ-----WLN--RKPKPINGFTNKSIRPKSLNVSSINT------QREFFGATSAPTTPVAH---SNGLHVL------ARSAPLSMSGNEYRAIMFLR---KVIEKKNGSLSLSHLFLYLGK-ASETIRNIIGWTKFELQEFIRKNSVFFETTPNEMILNKKANRINVIITGT----SSSCDPGTRTLTNRCGNVFHVAKLWGIIDLGKHEHVFFDKSIFRHLDDLQRHFKIGETLYFNAILAPKESRAKWRATQVWKESDREMVEKFSGNILSRTTTNSG 1776
            M + N +  LYFLE+L  S   L ++ + + +  K F     ++  N  +   LFL+ YPSLF+ +   V   + D        ++C S  L       TN   SS      F  ++  +LS+     +D  +E EAVK+FQS L K+++KWI IKSLAGHLSQA  NIR V+G Q  F  FL+RH HVFE+QGELV LKDNF S       +N  RK K      ++S++    N S  N       ++++F   +     +++   +N   +L        S  +++S  +Y+ IM+L    K IE+     +L++L + + + +S  I +I+G+T+ EL+E++ K+++FF+     ++     + +N+II+      S S +    +L+NR G V+HVAKLWGIIDLG+HEHVFFDKSIF+++++L+++F+IGE +YFNA+LA K+SRAKWRAT+VWKE+D  +++  + N+LS+  ++ G
Sbjct:    1 MAEHNLQLALYFLEMLY-SKGPLPLNLIAEVYQSKIFPHQA-LSYDNLINETVLFLKQYPSLFQTEAEFVFPASSD--------NSCHSEPL------PTNNIHSS-----RFINNR--RLSV---DDYDIALESEAVKYFQSHLLKKEDKWIPIKSLAGHLSQASDNIRLVIGNQSLFTAFLMRHSHVFEIQGELVGLKDNFMSNKSFSNSINGRRKTKGSQNMDSRSVKSDETNNSDSNKSNSGNGKKKYFRENNKNPEVMSNFILANASKILKGENNNQNSVTIALSAIDYKTIMYLHNTIKSIEETARECNLANLMVNIVRNSSNNITSIVGFTQIELEEYLIKHALFFDILDGAIVRVSDISNVNIIISDNCNLESLSNELENISLSNRKGIVYHVAKLWGIIDLGQHEHVFFDKSIFKNIENLEKYFQIGEKIYFNAVLASKDSRAKWRATRVWKEADNPILQD-NNNLLSKNPSDIG 457
The following BLAST results are available for this feature:
BLAST of Exonuclease 3'-5' domain-containing protein 1 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Exonuclease 3'-5' domain-containing protein 1 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 4
Match NameE-valueIdentityDescription
egal-11.650e-1425.14Egalitarian protein homolog [Source:UniProtKB/Swi... [more]
egal-11.650e-1425.14Egalitarian protein homolog [Source:UniProtKB/Swi... [more]
egal-11.698e-1425.14Egalitarian protein homolog [Source:UniProtKB/Swi... [more]
egal-11.698e-1425.14Egalitarian protein homolog [Source:UniProtKB/Swi... [more]
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BLAST of Exonuclease 3'-5' domain-containing protein 1 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 2
Match NameE-valueIdentityDescription
egl2.502e-1027.98gene:FBgn0000562 transcript:FBtr0343272[more]
egl2.502e-1027.98gene:FBgn0000562 transcript:FBtr0114586[more]
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BLAST of Exonuclease 3'-5' domain-containing protein 1 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Exonuclease 3'-5' domain-containing protein 1 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Exonuclease 3'-5' domain-containing protein 1 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Exonuclease 3'-5' domain-containing protein 1 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 1
Match NameE-valueIdentityDescription
sp|Q17902|EGAL1_CAEEL2.160e-1325.14Egalitarian protein homolog OS=Caenorhabditis eleg... [more]
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BLAST of Exonuclease 3'-5' domain-containing protein 1 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A267EF485.578e-13938.10Uncharacterized protein (Fragment) OS=Macrostomum ... [more]
A0A267E5851.647e-13344.12Uncharacterized protein (Fragment) OS=Macrostomum ... [more]
A0A1I8HNR92.429e-11443.31Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A210Q9351.083e-10442.52Uncharacterized protein OS=Mizuhopecten yessoensis... [more]
K1R3913.746e-10241.87Uncharacterized protein OS=Crassostrea gigas OX=29... [more]
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BLAST of Exonuclease 3'-5' domain-containing protein 1 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Exonuclease 3'-5' domain-containing protein 1 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Exonuclease 3'-5' domain-containing protein 1 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Exonuclease 3'-5' domain-containing protein 1 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Exonuclease 3'-5' domain-containing protein 1 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Exonuclease 3'-5' domain-containing protein 1 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000053185.10.000e+099.56SMESG000053185.1[more]
SMESG000053185.14.206e-3699.48SMESG000053185.1[more]
SMESG000027548.11.762e-14544.59SMESG000027548.1[more]
SMESG000027548.15.867e-14244.36SMESG000027548.1[more]
SMESG000028883.11.521e-7636.98SMESG000028883.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30023135 ID=SMED30023135|Name=Exonuclease 3'-5' domain-containing protein 1|organism=Schmidtea mediterranea sexual|type=transcript|length=2601bp
CATTTACATACACGTGTGAATGTTGGGCATTGGATAAAAAGAATACACCA
ATTGTATGTGAAAACCAAAAGCATGTGTGTATATCGAGTGTGTCAAAATG
ATCTGATAAGGTACGAATGGCAATGTAACAGTAACGATGGAAATCGATTG
ATTTATCAGCAATAAAGTGGTTAAAATTTTCTTTGGATTTTCGAGCATTT
TCTGATTTTTTCCAGATCAAATCAAAGAAATATTAATTAACGGTTTGTTT
AATCTTTCGATTGCTTCAAGAAAAGAACAATTCATTATCCGATTTAAACA
TTTCCACCAATGTCCGAGATATCGGATTCGGATTTATATGACAGTCAGAA
CTATGAAGACTTAGATTCGTGTTTGGGTTATTTGAAAAAACGTGAAGTCT
TTCTTTATAAAATGATGAATGATCCAAATCACAAAGCCATGCTCTATTTT
CTCGAAGTCCTTCTCAATTCAGATCACCAGTTGACAATCGACGAACTCGT
TGATCGATTCTACTGTAAAAACTTTACCGAAGATATGCGGATAGCGTGTG
GGAATAATGCTCACGGGTTGAAACTTTTTCTCGAATGCTATCCGTCGTTA
TTCAGAGTCAAAGGCAACAATGTCAGCGCGGTGACGATAGACATGCCGAA
TTCGGTTTCACTCATCAGTAATTGTTCGTCGCCTTCTTTAGTTTCCCAGA
GTAACAATACGACAAACGCCACTGTCAGTTCTGACAACACAGAAACCGAA
TTTACTTGCTCTAAAATATCCAAGCTCTCTCTGAAATTCCCTCAAAAATT
TGACTTTAACATGGAAATTGAAGCAGTGAAATTTTTTCAATCCAAATTAC
AAAAAAGAGATGAAAAATGGATTTTGATCAAGAGTCTTGCCGGGCATCTT
TCTCAAGCAGATATAAACATTCGAGAGGTTGTGGGACCACAAGTTGAATT
TAGGAAATTTCTTTTGCGCCATCCTCATGTTTTTGAGGTGCAAGGTGAAC
TGGTGAGTTTGAAAGATAATTTTCCTTCACAGTGGCTTAATCGTAAACCA
AAACCAATCAATGGATTCACTAACAAATCAATAAGACCTAAATCATTGAA
TGTATCAAGCATTAATACACAACGAGAGTTCTTTGGTGCTACTTCCGCTC
CGACTACGCCAGTTGCTCATAGTAATGGCTTGCATGTATTAGCTCGTTCA
GCTCCTTTGAGCATGTCCGGAAATGAGTACCGAGCAATTATGTTCTTGAG
AAAAGTGATTGAAAAAAAGAACGGATCCCTTAGTCTGTCACATTTGTTTC
TATACCTTGGCAAGGCCAGTGAAACAATTCGTAACATTATAGGATGGACC
AAGTTTGAATTGCAAGAATTCATTCGAAAGAATTCAGTATTTTTTGAAAC
AACACCCAACGAAATGATTCTTAACAAGAAAGCTAATAGAATAAACGTTA
TTATTACTGGAACTAGCAGTTCTTGTGATCCTGGTACAAGGACTCTTACA
AATAGATGTGGTAATGTATTTCACGTGGCAAAATTATGGGGCATCATAGA
TCTTGGCAAGCATGAGCATGTGTTTTTTGATAAGTCAATATTTAGACATC
TAGATGATTTGCAGCGTCATTTTAAGATTGGCGAAACTCTCTATTTCAAT
GCTATATTAGCGCCAAAAGAATCGAGAGCAAAATGGAGAGCAACGCAAGT
CTGGAAAGAGTCCGATAGGGAAATGGTTGAAAAATTCAGTGGGAATATTT
TATCAAGGACAACCACCAATTCAGGAATTAATAACGACTGCCTTTCAGAT
GCCAACAGTATCATAACCGATGCTTCTGACGATCTTACTCATTTTGCAAA
CGGCGAACCCAATAACAATACTAAAATGCTCCCTGAAGACGTAGAAGAAG
AGCTCACTAGAATTTTGAAATTGACAAAAACAGATTGTGAAAACGATGAA
GATTATTTTGCTAATTTTGACAAATTCTACAAAAAAGATGTGCTCATTGA
GTTCAATAAACCATTTAAAAATGATGCGGGTAAACTTTGTGATTTGGATT
ATGACGATGATTCTCAATCAATTGAAAGTATTCCGACTCCGGATTTCAGT
TCCCAAACAAGCGTCCAATCAGAACCTAGCGTTGCGTCTCATCCGTTATC
AGCTCCAGCAGCTTCATCGGATCATTATGCTATTCTTCAATCAACTCCAT
CAAACAAAAAATCAGTTTTCATCAAAATTGTTACAAAAATTCCCGAAGAA
CCTAAGCTACTTCAACCTGATAGTACGAAGAGTCCCAAAATGGTAAGCAT
CGCCACCCAAACCCTCTCTACTGGCGATATAATGGCTACCAATTTATTTC
ACGAGGGCATAAGTCAAATAGTCTGATTTCTCCCATGTGATTCGAATGTC
TGATCTCATGTAAATATTATTTTATACATATATACACAGAAATGAATGGT
TAGTCAATGGCTATTTCAGTCTTATAAACGTCAGTCATGGAAGTTTTATT
TGAAATCATCATTTTATTGGTTTCCTATTATTAGAAAATTAGATTATTAT
TTTTATTGATTATATATAATGTCACTATAATTGATTTTATAGAAATACAG
C
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protein sequence of SMED30023135-orf-1

>SMED30023135-orf-1 ID=SMED30023135-orf-1|Name=SMED30023135-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=689bp
MSEISDSDLYDSQNYEDLDSCLGYLKKREVFLYKMMNDPNHKAMLYFLEV
LLNSDHQLTIDELVDRFYCKNFTEDMRIACGNNAHGLKLFLECYPSLFRV
KGNNVSAVTIDMPNSVSLISNCSSPSLVSQSNNTTNATVSSDNTETEFTC
SKISKLSLKFPQKFDFNMEIEAVKFFQSKLQKRDEKWILIKSLAGHLSQA
DINIREVVGPQVEFRKFLLRHPHVFEVQGELVSLKDNFPSQWLNRKPKPI
NGFTNKSIRPKSLNVSSINTQREFFGATSAPTTPVAHSNGLHVLARSAPL
SMSGNEYRAIMFLRKVIEKKNGSLSLSHLFLYLGKASETIRNIIGWTKFE
LQEFIRKNSVFFETTPNEMILNKKANRINVIITGTSSSCDPGTRTLTNRC
GNVFHVAKLWGIIDLGKHEHVFFDKSIFRHLDDLQRHFKIGETLYFNAIL
APKESRAKWRATQVWKESDREMVEKFSGNILSRTTTNSGINNDCLSDANS
IITDASDDLTHFANGEPNNNTKMLPEDVEEELTRILKLTKTDCENDEDYF
ANFDKFYKKDVLIEFNKPFKNDAGKLCDLDYDDDSQSIESIPTPDFSSQT
SVQSEPSVASHPLSAPAASSDHYAILQSTPSNKKSVFIKIVTKIPEEPKL
LQPDSTKSPKMVSIATQTLSTGDIMATNLFHEGISQIV*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: molecular function
TermDefinition
GO:0004527exonuclease activity
Vocabulary: biological process
TermDefinition
GO:0090305nucleic acid phosphodiester bond hydrolysis
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000101muscle cell
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 592..614