Innexin

Overview
NameInnexin
Smed IDSMED30023100
Length (bp)619
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Innexin (SMED30023100) t-SNE clustered cells

Violin plots show distribution of expression levels for Innexin (SMED30023100) in cells (dots) of each of the 12 neoblast clusters.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30023100

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 2

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30023100SMESG000031896.1 SMESG000031895.1 OX_Smed_1.0.14398ox_Smed_v2PMID:24238224
Kao et al., 2013		whole organism asexual adult RNA-sequencing evidence
Category 2 cellSMED30023100SMESG000031896.1 SMESG000031895.1 dd_Smed_v4_15239_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Innexin vs. Ensembl Celegans
Match: inx-13 (Innexin [Source:UniProtKB/TrEMBL;Acc:O44887])

HSP 1 Score: 80.4925 bits (197), Expect = 1.020e-17
Identity = 55/156 (35.26%), Postives = 87/156 (55.77%), Query Frame = 2 
Query:  113 GIEDFADKFNFHFXXXXXXXXXXXXXXKQYVLKPISCYMSTDVGGXXXXXXXXXXCWIQGTIPISYNGKIPATEQXXXXXXXXXXXYYQWVPFALGLQCIMFYFPRLIWQLLCYNHTGTDIQHLVTSANQAVHSSEEARNKMVLYLAKSIAQTCYQ 580
            G +D  D+ N+++T ++L++  L ++ KQYV +PI C++     G          C++Q T  IS +  IP +E      E  +I YYQWVPF LGLQ I+FY P L W+L+ +N +G  ++ ++  A +A    E+ARN+     AKS     Y+
Sbjct:   15 GDDDSIDRLNYYWTPMLLVIFALTLSAKQYVGQPIQCWIPAQFTGAWEQYSENY-CFVQNTYFISPDKYIPDSE---IDREGAEIGYYQWVPFILGLQAILFYLPSLFWRLMNFN-SGVALKKMLFGAKKADRVDEKARNEA----AKSTGAHLYE 161
[Close]
BLAST of Innexin vs. Ensembl Celegans
Match: unc-7 (Innexin unc-7 [Source:UniProtKB/Swiss-Prot;Acc:Q03412])

HSP 1 Score: 74.3294 bits (181), Expect = 1.572e-15
Identity = 48/148 (32.43%), Postives = 78/148 (52.70%), Query Frame = 2 
Query:  119 EDFADKFNFHFXXXXXXXXXXXXXXKQYVLKPISCYMSTDVGGXXXXXXXXXXCWIQGTIPISYNGKIPATEQXXXXXXXXXXXYYQWVPFALGLQCIMFYFPRLIWQLLCYNHTGTDIQHLVTSANQAVHSSEEARNKMVLYLAKSI 562
            +DF DK N+++T  +L    L+V+ KQYV  PI C++                CW+Q T  +     IP   +E+     ++I YYQWVPF L ++ ++FY P ++W+ L Y H+G ++Q LV  A  A     E + + V  +A+ +
Sbjct:   19 DDFVDKLNYYYTTTILASFALLVSAKQYVGFPIQCWVPATFTDAMEQYTENY-CWVQNTYWVPMQEDIP---REIYSRRNRQIGYYQWVPFILAIEALLFYVPCILWRGLLYWHSGINLQGLVQMACDARLMDSEIKTRTVYTMARHM 162
[Close]
BLAST of Innexin vs. Ensembl Celegans
Match: unc-7 (Innexin unc-7 [Source:UniProtKB/Swiss-Prot;Acc:Q03412])

HSP 1 Score: 74.3294 bits (181), Expect = 1.653e-15
Identity = 48/148 (32.43%), Postives = 78/148 (52.70%), Query Frame = 2 
Query:  119 EDFADKFNFHFXXXXXXXXXXXXXXKQYVLKPISCYMSTDVGGXXXXXXXXXXCWIQGTIPISYNGKIPATEQXXXXXXXXXXXYYQWVPFALGLQCIMFYFPRLIWQLLCYNHTGTDIQHLVTSANQAVHSSEEARNKMVLYLAKSI 562
            +DF DK N+++T  +L    L+V+ KQYV  PI C++                CW+Q T  +     IP   +E+     ++I YYQWVPF L ++ ++FY P ++W+ L Y H+G ++Q LV  A  A     E + + V  +A+ +
Sbjct:   80 DDFVDKLNYYYTTTILASFALLVSAKQYVGFPIQCWVPATFTDAMEQYTENY-CWVQNTYWVPMQEDIP---REIYSRRNRQIGYYQWVPFILAIEALLFYVPCILWRGLLYWHSGINLQGLVQMACDARLMDSEIKTRTVYTMARHM 223
[Close]
BLAST of Innexin vs. Ensembl Celegans
Match: unc-7 (Innexin unc-7 [Source:UniProtKB/Swiss-Prot;Acc:Q03412])

HSP 1 Score: 74.3294 bits (181), Expect = 1.654e-15
Identity = 48/148 (32.43%), Postives = 78/148 (52.70%), Query Frame = 2 
Query:  119 EDFADKFNFHFXXXXXXXXXXXXXXKQYVLKPISCYMSTDVGGXXXXXXXXXXCWIQGTIPISYNGKIPATEQXXXXXXXXXXXYYQWVPFALGLQCIMFYFPRLIWQLLCYNHTGTDIQHLVTSANQAVHSSEEARNKMVLYLAKSI 562
            +DF DK N+++T  +L    L+V+ KQYV  PI C++                CW+Q T  +     IP   +E+     ++I YYQWVPF L ++ ++FY P ++W+ L Y H+G ++Q LV  A  A     E + + V  +A+ +
Sbjct:   93 DDFVDKLNYYYTTTILASFALLVSAKQYVGFPIQCWVPATFTDAMEQYTENY-CWVQNTYWVPMQEDIP---REIYSRRNRQIGYYQWVPFILAIEALLFYVPCILWRGLLYWHSGINLQGLVQMACDARLMDSEIKTRTVYTMARHM 236
[Close]
BLAST of Innexin vs. Ensembl Celegans
Match: unc-7 (Innexin unc-7 [Source:UniProtKB/Swiss-Prot;Acc:Q03412])

HSP 1 Score: 74.3294 bits (181), Expect = 1.714e-15
Identity = 48/148 (32.43%), Postives = 78/148 (52.70%), Query Frame = 2 
Query:  119 EDFADKFNFHFXXXXXXXXXXXXXXKQYVLKPISCYMSTDVGGXXXXXXXXXXCWIQGTIPISYNGKIPATEQXXXXXXXXXXXYYQWVPFALGLQCIMFYFPRLIWQLLCYNHTGTDIQHLVTSANQAVHSSEEARNKMVLYLAKSI 562
            +DF DK N+++T  +L    L+V+ KQYV  PI C++                CW+Q T  +     IP   +E+     ++I YYQWVPF L ++ ++FY P ++W+ L Y H+G ++Q LV  A  A     E + + V  +A+ +
Sbjct:   93 DDFVDKLNYYYTTTILASFALLVSAKQYVGFPIQCWVPATFTDAMEQYTENY-CWVQNTYWVPMQEDIP---REIYSRRNRQIGYYQWVPFILAIEALLFYVPCILWRGLLYWHSGINLQGLVQMACDARLMDSEIKTRTVYTMARHM 236
[Close]
BLAST of Innexin vs. UniProt/SwissProt
Match: sp|Q03412|UNC7_CAEEL (Innexin unc-7 OS=Caenorhabditis elegans OX=6239 GN=unc-7 PE=1 SV=1)

HSP 1 Score: 74.3294 bits (181), Expect = 2.560e-14
Identity = 48/148 (32.43%), Postives = 78/148 (52.70%), Query Frame = 2 
Query:  119 EDFADKFNFHFXXXXXXXXXXXXXXKQYVLKPISCYMSTDVGGXXXXXXXXXXCWIQGTIPISYNGKIPATEQXXXXXXXXXXXYYQWVPFALGLQCIMFYFPRLIWQLLCYNHTGTDIQHLVTSANQAVHSSEEARNKMVLYLAKSI 562
            +DF DK N+++T  +L    L+V+ KQYV  PI C++                CW+Q T  +     IP   +E+     ++I YYQWVPF L ++ ++FY P ++W+ L Y H+G ++Q LV  A  A     E + + V  +A+ +
Sbjct:  139 DDFVDKLNYYYTTTILASFALLVSAKQYVGFPIQCWVPATFTDAMEQYTENY-CWVQNTYWVPMQEDIP---REIYSRRNRQIGYYQWVPFILAIEALLFYVPCILWRGLLYWHSGINLQGLVQMACDARLMDSEIKTRTVYTMARHM 282
[Close]
BLAST of Innexin vs. UniProt/SwissProt
Match: sp|O01393|UNC9_CAEEL (Innexin unc-9 OS=Caenorhabditis elegans OX=6239 GN=unc-9 PE=1 SV=1)

HSP 1 Score: 68.9366 bits (167), Expect = 1.547e-12
Identity = 49/153 (32.03%), Postives = 85/153 (55.56%), Query Frame = 2 
Query:  119 EDFADKFNFHFXXXXXXXXXXXXXXKQYVLKPISCYMSTDVGGXXXXXXXXXXCWIQGTIPISYNGKIPATEQXXXXXXXXXXXYYQWVPFALGLQCIMFYFPRLIWQLLCYNHTGTDIQHLVTSANQAVHSSEEARNKMVLYLAKSIAQTCY 577
            +D  DK N+++T  ++ +  ++V+ KQYV  PI C++        +  Y ENYCW+Q T  +  +  IP         E ++I YYQWVPF L L+ ++FY P ++W+LL +  +G  +Q LV  A  +     E+RN+ +  +A ++ +  +
Sbjct:   21 DDIIDKLNYYYTTAIITVFAILVSAKQYVGFPIQCWVPATFT-EPMEQYTENYCWVQNTYFLPLHDYIP---HNYAERENRQIGYYQWVPFVLALEALLFYVPTIVWRLLSW-QSGIHVQSLVQMACDSRLLDLESRNRALQTIATNVEEALH 168
[Close]
BLAST of Innexin vs. UniProt/SwissProt
Match: sp|Q23157|INX11_CAEEL (Innexin-11 OS=Caenorhabditis elegans OX=6239 GN=inx-11 PE=3 SV=1)

HSP 1 Score: 63.5438 bits (153), Expect = 1.120e-10
Identity = 42/149 (28.19%), Postives = 73/149 (48.99%), Query Frame = 2 
Query:  119 EDFADKFNFHFXXXXXXXXXXXXXXKQYVLKPISCYMSTDVGGXXXXXXXXXXCWIQGTIPISYNGKIPATEQXXXXXXXXXXXYYQWVPFALGLQCIMFYFPRLIWQLLCYNHTGTDIQHLVTSANQAVHSSEEARNKMVLYLAKSIA 565
            +D++D+ N+  T  +LL  +++++ KQ+  +PI C       G          CW Q T  +     +   ++E     ++++ YYQWVPF L LQ   F  P  +W+    NH+G  I  +V  A  + +  EE R K +L L + ++
Sbjct:   20 DDWSDRLNYLMTPNILLAFSVLISFKQFGGRPIECMFPNKFPGSWEQYAENY-CWSQDTYFVEPTQDVSLLKKEERYTPDRQLSYYQWVPFFLLLQAAFFRAPSYLWKYFS-NHSGIRIHEVVEKAKDSANVEEEVREKNILILKRHLS 166
[Close]
BLAST of Innexin vs. UniProt/SwissProt
Match: sp|Q27295|EAT5_CAEEL (Innexin eat-5 OS=Caenorhabditis elegans OX=6239 GN=eat-5 PE=2 SV=1)

HSP 1 Score: 60.8474 bits (146), Expect = 1.051e-9
Identity = 38/153 (24.84%), Postives = 75/153 (49.02%), Query Frame = 2 
Query:  128 ADKFNFHFXXXXXXXXXXXXXXKQYVLKPISCYMSTDVGGXXXXXXXXXXCWIQGTIPISYNGKIPATEQXXXXXXXXXXXYYQWVPFALGLQCIMFYFPRLIWQLLCYNHTGTDIQHLVTSANQAVHSSEEARNKMVLYLAKSIAQTCYQHR 586
             D+ N++++ ++++  +L +T +QYV  P+ C++             +  C++  T  +  N K+P T +E    +     YYQW PF + ++   FY P + W +L    +G +I  LV +A +A  +  E R K +  + + I+    + R
Sbjct:   20 TDRLNYYYSTLIIMGMSLTITARQYVGSPLQCWVPAQFTKAWEQYAEDY-CFVYNTYWVKPNDKVPLTVEERVSQQLI---YYQWAPFIMAIEAAFFYLPVIFWSMLS-TKSGINIIKLVETAQKAEGAESEERKKQIDIICRHISNNLRKRR 167
[Close]
BLAST of Innexin vs. UniProt/SwissProt
Match: sp|Q19746|INX3_CAEEL (Innexin-3 OS=Caenorhabditis elegans OX=6239 GN=inx-3 PE=1 SV=2)

HSP 1 Score: 59.6918 bits (143), Expect = 1.954e-9
Identity = 41/125 (32.80%), Postives = 53/125 (42.40%), Query Frame = 2 
Query:  194 KQYVLKPISCYMSTDVGGXXXXXXXXXXCWIQGTIPISYNGKIPATEQXXXXXXXXXXXYYQWVPFALGLQCIMFYFPRLIWQLLCYNHTGTDIQHLVTSANQAVHSSEEARNKMVLYLAKSIAQ 568
            KQYV   I C+M  +  G       +  C+IQ T  I    +IP   ++ +  E     YYQWVP  L +Q  MFY P  IW  L Y   G D   +++ A        E R K V  L   I  
Sbjct:   46 KQYVGSAIQCWMPMEFKGGWEQYAEDY-CFIQNTFFIPERSEIPGDVEDRQKAEIG---YYQWVPIVLAIQAFMFYLPSWIWSSL-YKQCGLDFPSVISEAEALRSQDSETRTKGVNKLVDFIGD 165
[Close]
BLAST of Innexin vs. TrEMBL
Match: A0A4S2LNY2 (Innexin OS=Opisthorchis felineus OX=147828 GN=inx PE=3 SV=1)

HSP 1 Score: 232.261 bits (591), Expect = 4.821e-71
Identity = 123/188 (65.43%), Postives = 157/188 (83.51%), Query Frame = 2 
Query:   56 MVALEFLDFLSKFQVTNYAGIEDFADKFNFHFXXXXXXXXXXXXXXKQYVLKPISCYMSTDVGGXXXXXXXXXXCWIQGTIPISYNGKIPATEQXXXXXXXXXXXYYQWVPFALGLQCIMFYFPRLIWQLLCYNHTGTDIQHLVTSANQAVHSSEEARNKMVLYLAKSIAQTCYQHREYRKDALANFR 619
            MV  EFLD++SKFQV  Y G+EDFADKFNF  TV++LLL T VVTVKQY++KPISCYM+TD+GG NLL+Y+ENYCW+QGTIPI+Y GK+P T+   E +E+ K+LYYQWVPF LGLQCIMFY PR+IWQ++CYN TGTDIQHLV SANQAVH++++ R KM+ ++A+++ Q  +QHREYR D  ++ R
Sbjct:    1 MVGSEFLDYISKFQVATYVGVEDFADKFNFLITVMILLLCTTVVTVKQYMMKPISCYMATDIGGKNLLDYVENYCWVQGTIPIAYAGKMPETDAAWEEMEKHKLLYYQWVPFVLGLQCIMFYVPRVIWQMICYNRTGTDIQHLVLSANQAVHATDDQRTKMIQHVARTLEQMLFQHREYRNDVWSHIR 188
[Close]
BLAST of Innexin vs. TrEMBL
Match: G7YJJ0 (Innexin OS=Clonorchis sinensis OX=79923 GN=inx PE=3 SV=1)

HSP 1 Score: 231.876 bits (590), Expect = 6.806e-71
Identity = 123/188 (65.43%), Postives = 157/188 (83.51%), Query Frame = 2 
Query:   56 MVALEFLDFLSKFQVTNYAGIEDFADKFNFHFXXXXXXXXXXXXXXKQYVLKPISCYMSTDVGGXXXXXXXXXXCWIQGTIPISYNGKIPATEQXXXXXXXXXXXYYQWVPFALGLQCIMFYFPRLIWQLLCYNHTGTDIQHLVTSANQAVHSSEEARNKMVLYLAKSIAQTCYQHREYRKDALANFR 619
            MV  EFLD++SKFQV  Y G+EDFADKFNF  TV++LLL T VVTVKQY++KPISCYM+TD+GG NLL+Y+ENYCW+QGTIPI+Y GK+P T+   E +E+ K+LYYQWVPF LGLQCIMFY PR+IWQ++CYN TGTDIQHLV SANQAVH++++ R KM+ ++A+++ Q  +QHREYR D  ++ R
Sbjct:    1 MVGSEFLDYISKFQVATYVGVEDFADKFNFLVTVMILLLCTTVVTVKQYMMKPISCYMATDIGGKNLLDYVENYCWVQGTIPIAYAGKMPETDAAWEEMEKHKLLYYQWVPFVLGLQCIMFYVPRVIWQMICYNRTGTDIQHLVLSANQAVHATDDQRTKMIQHVARTLEQMLFQHREYRNDVWSHIR 188
[Close]
BLAST of Innexin vs. TrEMBL
Match: A0A183JFJ2 (Innexin OS=Schistosoma curassoni OX=6186 GN=inx PE=3 SV=1)

HSP 1 Score: 214.157 bits (544), Expect = 3.566e-67
Identity = 113/185 (61.08%), Postives = 148/185 (80.00%), Query Frame = 2 
Query:   56 MVALEFLDFLSKFQVTNYAGIEDFADKFNFHFXXXXXXXXXXXXXXKQYVLKPISCYMSTDVGGXXXXXXXXXXCWIQGTIPISYNGKIPATEQXXXXXXXXXXXYYQWVPFALGLQCIMFYFPRLIWQLLCYNHTGTDIQHLVTSANQAVHSSEEARNKMVLYLAKSIAQTCYQHREYRKDALA 610
            M+  EFLD+LSKFQV  Y G+EDFADKFNF  TV+VL+L T +VT+KQY++KPISCYM+TD+GG NLL+Y+ENYCW+QGT+PI+Y+G++P T++    LE+ K+LYYQWVPF LGLQCI+FY PRLIWQ++CYN  GTD+ HLV  ANQAVH+++E R KMV +LAK++ Q  +Q      + L 
Sbjct:    1 MIGTEFLDYLSKFQVATYVGVEDFADKFNFLITVMVLMLCTTIVTIKQYMMKPISCYMATDLGGKNLLDYVENYCWVQGTVPIAYSGRVPETDEGWAELEKHKLLYYQWVPFVLGLQCILFYLPRLIWQMICYNRVGTDVHHLVLCANQAVHANDEQRTKMVQHLAKTLEQLLFQAETNLDEVLV 185
[Close]
BLAST of Innexin vs. TrEMBL
Match: A0A430QT10 (Innexin OS=Schistosoma bovis OX=6184 GN=inx PE=3 SV=1)

HSP 1 Score: 213.386 bits (542), Expect = 3.944e-67
Identity = 112/175 (64.00%), Postives = 146/175 (83.43%), Query Frame = 2 
Query:   56 MVALEFLDFLSKFQVTNYAGIEDFADKFNFHFXXXXXXXXXXXXXXKQYVLKPISCYMSTDVGGXXXXXXXXXXCWIQGTIPISYNGKIPATEQXXXXXXXXXXXYYQWVPFALGLQCIMFYFPRLIWQLLCYNHTGTDIQHLVTSANQAVHSSEEARNKMVLYLAKSIAQTCYQ 580
            M+  EFLD+LSKFQV  Y G+EDFADKFNF  TV+VL+L T +VT+KQY++KPISCYM+TD+GG NLL+Y+ENYCW+QGT+PI+Y+G++P T++    LE+ K+LYYQWVPF LGLQCI+FY PRLIWQ++CYN  GTD+ HLV  ANQAVH+++E R KMV +LAK++ Q  +Q
Sbjct:    1 MIGTEFLDYLSKFQVATYVGVEDFADKFNFLITVMVLMLCTTIVTIKQYMMKPISCYMATDLGGKNLLDYVENYCWVQGTVPIAYSGRVPETDEGWAELEKHKLLYYQWVPFVLGLQCILFYLPRLIWQMICYNRVGTDVHHLVLCANQAVHANDEQRTKMVQHLAKTLEQLLFQ 175
[Close]
BLAST of Innexin vs. TrEMBL
Match: C1LF30 (Innexin OS=Schistosoma japonicum OX=6182 GN=inx PE=2 SV=1)

HSP 1 Score: 220.705 bits (561), Expect = 3.500e-66
Identity = 116/179 (64.80%), Postives = 149/179 (83.24%), Query Frame = 2 
Query:   56 MVALEFLDFLSKFQVTNYAGIEDFADKFNFHFXXXXXXXXXXXXXXKQYVLKPISCYMSTDVGGXXXXXXXXXXCWIQGTIPISYNGKIPATEQXXXXXXXXXXXYYQWVPFALGLQCIMFYFPRLIWQLLCYNHTGTDIQHLVTSANQAVHSSEEARNKMVLYLAKSIAQTCYQHREY 592
            M+  EFLD+LSKFQ+  Y G+EDFADKFNF  TV+VL++ T +VTVKQY++KPISCYM+TD+GG NLL+Y+ENYCW+QGTIPISY+G++P T++    LE+ K+LYYQWVPF LGLQCI+FY PRLIWQ++CYN  GTD+QHLV  ANQAVH+ ++ R KMV +LAK++ Q  +Q REY
Sbjct:    1 MIGTEFLDYLSKFQIATYVGVEDFADKFNFLITVMVLMICTTIVTVKQYMIKPISCYMATDLGGKNLLDYVENYCWVQGTIPISYSGRVPETDEGWAELEKHKLLYYQWVPFVLGLQCILFYLPRLIWQMICYNRVGTDVQHLVLCANQAVHAGDDQRTKMVQHLAKTLEQLLFQQREY 179
[Close]
BLAST of Innexin vs. Planmine SMEST
Match: SMESG000069482.1 (SMESG000069482.1)

HSP 1 Score: 238.81 bits (608), Expect = 2.716e-80
Identity = 128/188 (68.09%), Postives = 152/188 (80.85%), Query Frame = 2 
Query:   56 MVALEFLDFLSKFQVTNYAGIEDFADKFNFHFXXXXXXXXXXXXXXKQYVLKPISCYMSTDVGGXXXXXXXXXXCWIQGTIPISYNGKIPATEQXXXXXXXXXXXYYQWVPFALGLQCIMFYFPRLIWQLLCYNHTGTDIQHLVTSANQAVHSSEEARNKMVLYLAKSIAQTCYQHREYRKDALANFR 619
            MVA EFLD +SKFQ+TNY G+EDFADKFNF  T IV  +   VV+VK YVLKPISCY++ D  G NLLNY+ENYCW+QGT+PI Y+GKIP  + E   L++ KILYYQWVPF L LQCIMFY PR+IWQ+ CYN TGTDIQHL+TSAN AVHSSEEAR KMV++LAKS+ Q  +QHR++R D +ANFR
Sbjct:    1 MVAQEFLDQISKFQITNYVGVEDFADKFNFLLTTIVFSICASVVSVKLYVLKPISCYLAVDSSGTNLLNYVENYCWVQGTVPIKYDGKIPTNDAEWNQLQKNKILYYQWVPFVLSLQCIMFYIPRIIWQMFCYNRTGTDIQHLITSANHAVHSSEEARKKMVIHLAKSLEQLLFQHRDFRNDKIANFR 188
[Close]
BLAST of Innexin vs. Planmine SMEST
Match: SMESG000065083.1 (SMESG000065083.1)

HSP 1 Score: 222.246 bits (565), Expect = 7.722e-71
Identity = 120/188 (63.83%), Postives = 152/188 (80.85%), Query Frame = 2 
Query:   56 MVALEFLDFLSKFQVTNYAGIEDFADKFNFHFXXXXXXXXXXXXXXKQYVLKPISCYMSTDVGGXXXXXXXXXXCWIQGTIPISYNGKIPATEQXXXXXXXXXXXYYQWVPFALGLQCIMFYFPRLIWQLLCYNHTGTDIQHLVTSANQAVHSSEEARNKMVLYLAKSIAQTCYQHREYRKDALANFR 619
            MVA EFLD+LSK QV+   G+ED  D+ NF F+VIVL++  L+VTVKQYV KPISCY+  ++GG NLL+Y+EN CW+QGTI +S   K P+T++E + LE+ K+LYYQWVPF LGLQCIMFY P+L+WQ++CYN TGTDIQHLV SANQAVHSS+EAR+KMV YL+K++ Q  YQHR++R   LA FR
Sbjct:    1 MVASEFLDYLSKLQVSTNVGLEDLPDRLNFTFSVIVLMVCILIVTVKQYVFKPISCYLPNNIGGSNLLSYVENLCWVQGTIALSNQAKFPSTDEEWKNLEKHKMLYYQWVPFMLGLQCIMFYVPKLVWQIICYNRTGTDIQHLVVSANQAVHSSDEARSKMVSYLSKTLEQLLYQHRDHRSGKLAKFR 188
[Close]
BLAST of Innexin vs. Planmine SMEST
Match: SMESG000070656.1 (SMESG000070656.1)

HSP 1 Score: 182.57 bits (462), Expect = 9.793e-56
Identity = 98/142 (69.01%), Postives = 114/142 (80.28%), Query Frame = 2 
Query:  194 KQYVLKPISCYMSTDVGGXXXXXXXXXXCWIQGTIPISYNGKIPATEQXXXXXXXXXXXYYQWVPFALGLQCIMFYFPRLIWQLLCYNHTGTDIQHLVTSANQAVHSSEEARNKMVLYLAKSIAQTCYQHREYRKDALANFR 619
            KQY+LKPISCY+ST + G NLL Y+ENYCW++GTIPISY+GKIP+T +E   L + KILYYQWVPF L LQ IMFY PRLIWQ+ CYN TGTDI HL+ SANQAVHSS+EAR KMV Y+AK+I Q  YQHR+ R   LA FR
Sbjct:    2 KQYILKPISCYIST-ISGTNLLEYVENYCWVEGTIPISYDGKIPSTNEEWAELNKYKILYYQWVPFLLSLQAIMFYIPRLIWQIFCYNTTGTDIHHLIVSANQAVHSSDEARTKMVNYIAKTIEQMLYQHRDNRNGRLARFR 142
[Close]
BLAST of Innexin vs. Planmine SMEST
Match: SMESG000080788.1 (SMESG000080788.1)

HSP 1 Score: 155.992 bits (393), Expect = 2.380e-45
Identity = 87/189 (46.03%), Postives = 126/189 (66.67%), Query Frame = 2 
Query:   56 MVALEFLDFLSKFQVTNYAGIEDFADKFNFHFXXXXXXXXXXXXXXKQYVLKPISCYMSTDVGGXXXXXXXXXXCWIQGTIPISYNGKIPATE-QXXXXXXXXXXXYYQWVPFALGLQCIMFYFPRLIWQLLCYNHTGTDIQHLVTSANQAVHSSEEARNKMVLYLAKSIAQTCYQHREYRKDALANFR 619
            MVA EFL+ + +F+++ Y GIEDFADK +F F+V +L+LS +VVT+K Y+ KP++CY +T  GG N  NYLENYCW+ GTI       IP  +       + ++I YYQWVPF LGLQC MFY P++IWQ++CYN +GT+++ +V  AN A ++    R   +  +A++I    +QHR+YR+   A  R
Sbjct:    1 MVAEEFLNLIGQFKISTYVGIEDFADKLSFLFSVTLLILSMMVVTIKSYLFKPLACYTATAPGGSNFENYLENYCWVHGTITFLPGEDIPQNDVDWARADDTRRINYYQWVPFILGLQCFMFYIPKIIWQIICYNKSGTNLESIVNDANAANNTPPVERKDKIERIARTIEDMLFQHRDYRQGNFAKLR 189
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BLAST of Innexin vs. Planmine SMEST
Match: SMESG000031895.1 (SMESG000031895.1)

HSP 1 Score: 145.976 bits (367), Expect = 3.439e-45
Identity = 94/94 (100.00%), Postives = 94/94 (100.00%), Query Frame = 2 
Query:   56 MVALEFLDFLSKFQVTNYAGIEDFADKFNFHFXXXXXXXXXXXXXXKQYVLKPISCYMSTDVGGXXXXXXXXXXCWIQGTIPISYNGKIPATEQ 337
            MVALEFLDFLSKFQVTNYAGIEDFADKFNFHFTVIVLLLSTLVVTVKQYVLKPISCYMSTDVGGLNLLNYLENYCWIQGTIPISYNGKIPATEQ
Sbjct:    1 MVALEFLDFLSKFQVTNYAGIEDFADKFNFHFTVIVLLLSTLVVTVKQYVLKPISCYMSTDVGGLNLLNYLENYCWIQGTIPISYNGKIPATEQ 94
The following BLAST results are available for this feature:
BLAST of Innexin vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Innexin vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
inx-131.020e-1735.26Innexin [Source:UniProtKB/TrEMBL;Acc:O44887][more]
unc-71.572e-1532.43Innexin unc-7 [Source:UniProtKB/Swiss-Prot;Acc:Q0... [more]
unc-71.653e-1532.43Innexin unc-7 [Source:UniProtKB/Swiss-Prot;Acc:Q0... [more]
unc-71.654e-1532.43Innexin unc-7 [Source:UniProtKB/Swiss-Prot;Acc:Q0... [more]
unc-71.714e-1532.43Innexin unc-7 [Source:UniProtKB/Swiss-Prot;Acc:Q0... [more]
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BLAST of Innexin vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Innexin vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Innexin vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Innexin vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Innexin vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q03412|UNC7_CAEEL2.560e-1432.43Innexin unc-7 OS=Caenorhabditis elegans OX=6239 GN... [more]
sp|O01393|UNC9_CAEEL1.547e-1232.03Innexin unc-9 OS=Caenorhabditis elegans OX=6239 GN... [more]
sp|Q23157|INX11_CAEEL1.120e-1028.19Innexin-11 OS=Caenorhabditis elegans OX=6239 GN=in... [more]
sp|Q27295|EAT5_CAEEL1.051e-924.84Innexin eat-5 OS=Caenorhabditis elegans OX=6239 GN... [more]
sp|Q19746|INX3_CAEEL1.954e-932.80Innexin-3 OS=Caenorhabditis elegans OX=6239 GN=inx... [more]
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BLAST of Innexin vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A4S2LNY24.821e-7165.43Innexin OS=Opisthorchis felineus OX=147828 GN=inx ... [more]
G7YJJ06.806e-7165.43Innexin OS=Clonorchis sinensis OX=79923 GN=inx PE=... [more]
A0A183JFJ23.566e-6761.08Innexin OS=Schistosoma curassoni OX=6186 GN=inx PE... [more]
A0A430QT103.944e-6764.00Innexin OS=Schistosoma bovis OX=6184 GN=inx PE=3 S... [more]
C1LF303.500e-6664.80Innexin OS=Schistosoma japonicum OX=6182 GN=inx PE... [more]
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BLAST of Innexin vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Innexin vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Innexin vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Innexin vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Innexin vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Innexin vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000069482.12.716e-8068.09SMESG000069482.1[more]
SMESG000065083.17.722e-7163.83SMESG000065083.1[more]
SMESG000070656.19.793e-5669.01SMESG000070656.1[more]
SMESG000080788.12.380e-4546.03SMESG000080788.1[more]
SMESG000031895.13.439e-45100.00SMESG000031895.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30023100 ID=SMED30023100|Name=Innexin|organism=Schmidtea mediterranea sexual|type=transcript|length=619bp
CTTAGTGGAAATGAGAATCCATTTTGACCATTTCTAAAAACAATTCGCCA
TTCTAATGGTTGCCCTTGAATTTCTGGACTTCCTCAGTAAGTTCCAAGTA
ACAAATTATGCTGGAATAGAGGATTTTGCGGATAAATTTAATTTCCATTT
CACTGTCATCGTTTTGTTGTTGTCTACCCTTGTTGTTACAGTGAAACAAT
ATGTATTGAAACCGATCTCATGCTACATGTCGACAGATGTCGGAGGATTA
AATTTGCTGAACTATTTGGAGAACTATTGCTGGATCCAAGGAACGATACC
AATATCATACAATGGGAAAATTCCAGCGACTGAACAGGAGTTGGAAATTT
TAGAAGAAAAAAAAATACTTTATTATCAATGGGTTCCTTTTGCTTTGGGT
TTGCAATGTATAATGTTCTACTTTCCGAGACTGATTTGGCAACTTTTATG
TTATAACCACACAGGAACCGACATTCAGCATCTTGTCACAAGTGCCAACC
AAGCGGTTCACTCAAGTGAAGAGGCTAGGAACAAAATGGTCCTTTATTTA
GCAAAATCAATAGCCCAAACATGTTATCAACATCGTGAATATCGAAAGGA
TGCTTTAGCCAATTTTCGG
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protein sequence of SMED30023100-orf-1

>SMED30023100-orf-1 ID=SMED30023100-orf-1|Name=SMED30023100-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=188bp
MVALEFLDFLSKFQVTNYAGIEDFADKFNFHFTVIVLLLSTLVVTVKQYV
LKPISCYMSTDVGGLNLLNYLENYCWIQGTIPISYNGKIPATEQELEILE
EKKILYYQWVPFALGLQCIMFYFPRLIWQLLCYNHTGTDIQHLVTSANQA
VHSSEEARNKMVLYLAKSIAQTCYQHREYRKDALANFR
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000028Category 2 cell
PLANA:0000418head
Vocabulary: INTERPRO
TermDefinition
IPR000990Innexin
Vocabulary: cellular component
TermDefinition
GO:0005921gap junction
GO:0005886plasma membrane
GO:0016020membrane
GO:0016021integral component of membrane
GO:0030054cell junction
Vocabulary: biological process
TermDefinition
GO:0006810transport
GO:0006811ion transport