Non-specific serine/threonine protein kinase
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30023085 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 8
Homology
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Human
Match: RPS6KB1 (ribosomal protein S6 kinase B1 [Source:HGNC Symbol;Acc:HGNC:10436]) HSP 1 Score: 407.142 bits (1045), Expect = 2.979e-135 Identity = 202/357 (56.58%), Postives = 262/357 (73.39%), Query Frame = 2
Query: 215 ESVEICQSVVNPGV-KISMEDFKVLNVIGRGGYGKVFLVQKSSSGHDENKLYAMKVLKKARIIRNEKDTAHTKSERNILEMIRHPFLVKLHYAFQNQSNLYIVLTYCSGGELFTYLEREGTLMENQACFYVAEISLAIGHLHKTGIIYRDLKPENVLLDKKGHVKLTDFGLSKESV---SLTNTFCGTIEYMAPEILKRGGHGKEVDWWSLGTLLFDMLSGGPPFSQECDKQETITKILHSTIAYPKYFSSEVVRLIQGLLKRNPKERLGC-KNDVEDIKNHEFFKTHDINWSDVYHRRMDPPFKPELKSEMDVSCFDSTFTGQQPIMTPDDCNGAHILPDLFQGFSYVAPNVASSV 1270
E EI ++ VN G KI E F++L V+G+GGYGKVF V+K + G + K++AMKVLKKA I+RN KDTAHTK+ERNILE ++HPF+V L YAFQ LY++L Y SGGELF LEREG ME+ ACFY+AEIS+A+GHLH+ GIIYRDLKPEN++L+ +GHVKLTDFGL KES+ ++T+TFCGTIEYMAPEIL R GH + VDWWSLG L++DML+G PPF+ E ++++TI KIL + P Y + E L++ LLKRN RLG D +++ H FF+ INW ++ R+++PPFKP L+SE DVS FDS FT Q P+ +PDD + +F GF+YVAP+V SV
Sbjct: 17 EKFEISETSVNRGPEKIRPECFELLRVLGKGGYGKVFQVRKVT-GANTGKIFAMKVLKKAMIVRNAKDTAHTKAERNILEEVKHPFIVDLIYAFQTGGKLYLILEYLSGGELFMQLEREGIFMEDTACFYLAEISMALGHLHQKGIIYRDLKPENIMLNHQGHVKLTDFGLCKESIHDGTVTHTFCGTIEYMAPEILMRSGHNRAVDWWSLGALMYDMLTGAPPFTGE-NRKKTIDKILKCKLNLPPYLTQEARDLLKKLLKRNAASRLGAGPGDAGEVQAHPFFR--HINWEELLARKVEPPFKPLLQSEEDVSQFDSKFTRQTPVDSPDDSTLSESANQVFLGFTYVAPSVLESV 369
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Human
Match: RPS6KB1 (ribosomal protein S6 kinase B1 [Source:HGNC Symbol;Acc:HGNC:10436]) HSP 1 Score: 405.601 bits (1041), Expect = 5.010e-135 Identity = 202/357 (56.58%), Postives = 262/357 (73.39%), Query Frame = 2
Query: 215 ESVEICQSVVNPG-VKISMEDFKVLNVIGRGGYGKVFLVQKSSSGHDENKLYAMKVLKKARIIRNEKDTAHTKSERNILEMIRHPFLVKLHYAFQNQSNLYIVLTYCSGGELFTYLEREGTLMENQACFYVAEISLAIGHLHKTGIIYRDLKPENVLLDKKGHVKLTDFGLSKESV---SLTNTFCGTIEYMAPEILKRGGHGKEVDWWSLGTLLFDMLSGGPPFSQECDKQETITKILHSTIAYPKYFSSEVVRLIQGLLKRNPKERLGCK-NDVEDIKNHEFFKTHDINWSDVYHRRMDPPFKPELKSEMDVSCFDSTFTGQQPIMTPDDCNGAHILPDLFQGFSYVAPNVASSV 1270
E EI ++ VN G KI E F++L V+G+GGYGKVF V+K + G + K++AMKVLKKA I+RN KDTAHTK+ERNILE ++HPF+V L YAFQ LY++L Y SGGELF LEREG ME+ ACFY+AEIS+A+GHLH+ GIIYRDLKPEN++L+ +GHVKLTDFGL KES+ ++T+TFCGTIEYMAPEIL R GH + VDWWSLG L++DML+G PPF+ E ++++TI KIL + P Y + E L++ LLKRN RLG D +++ H FF+ INW ++ R+++PPFKP L+SE DVS FDS FT Q P+ +PDD + +F GF+YVAP+V SV
Sbjct: 70 EKFEISETSVNRGPEKIRPECFELLRVLGKGGYGKVFQVRKVT-GANTGKIFAMKVLKKAMIVRNAKDTAHTKAERNILEEVKHPFIVDLIYAFQTGGKLYLILEYLSGGELFMQLEREGIFMEDTACFYLAEISMALGHLHQKGIIYRDLKPENIMLNHQGHVKLTDFGLCKESIHDGTVTHTFCGTIEYMAPEILMRSGHNRAVDWWSLGALMYDMLTGAPPFTGE-NRKKTIDKILKCKLNLPPYLTQEARDLLKKLLKRNAASRLGAGPGDAGEVQAHPFFR--HINWEELLARKVEPPFKPLLQSEEDVSQFDSKFTRQTPVDSPDDSTLSESANQVFLGFTYVAPSVLESV 422
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Human
Match: RPS6KB1 (ribosomal protein S6 kinase B1 [Source:HGNC Symbol;Acc:HGNC:10436]) HSP 1 Score: 407.142 bits (1045), Expect = 1.672e-134 Identity = 202/357 (56.58%), Postives = 262/357 (73.39%), Query Frame = 2
Query: 215 ESVEICQSVVNPGV-KISMEDFKVLNVIGRGGYGKVFLVQKSSSGHDENKLYAMKVLKKARIIRNEKDTAHTKSERNILEMIRHPFLVKLHYAFQNQSNLYIVLTYCSGGELFTYLEREGTLMENQACFYVAEISLAIGHLHKTGIIYRDLKPENVLLDKKGHVKLTDFGLSKESV---SLTNTFCGTIEYMAPEILKRGGHGKEVDWWSLGTLLFDMLSGGPPFSQECDKQETITKILHSTIAYPKYFSSEVVRLIQGLLKRNPKERLGC-KNDVEDIKNHEFFKTHDINWSDVYHRRMDPPFKPELKSEMDVSCFDSTFTGQQPIMTPDDCNGAHILPDLFQGFSYVAPNVASSV 1270
E EI ++ VN G KI E F++L V+G+GGYGKVF V+K + G + K++AMKVLKKA I+RN KDTAHTK+ERNILE ++HPF+V L YAFQ LY++L Y SGGELF LEREG ME+ ACFY+AEIS+A+GHLH+ GIIYRDLKPEN++L+ +GHVKLTDFGL KES+ ++T+TFCGTIEYMAPEIL R GH + VDWWSLG L++DML+G PPF+ E ++++TI KIL + P Y + E L++ LLKRN RLG D +++ H FF+ INW ++ R+++PPFKP L+SE DVS FDS FT Q P+ +PDD + +F GF+YVAP+V SV
Sbjct: 70 EKFEISETSVNRGPEKIRPECFELLRVLGKGGYGKVFQVRKVT-GANTGKIFAMKVLKKAMIVRNAKDTAHTKAERNILEEVKHPFIVDLIYAFQTGGKLYLILEYLSGGELFMQLEREGIFMEDTACFYLAEISMALGHLHQKGIIYRDLKPENIMLNHQGHVKLTDFGLCKESIHDGTVTHTFCGTIEYMAPEILMRSGHNRAVDWWSLGALMYDMLTGAPPFTGE-NRKKTIDKILKCKLNLPPYLTQEARDLLKKLLKRNAASRLGAGPGDAGEVQAHPFFR--HINWEELLARKVEPPFKPLLQSEEDVSQFDSKFTRQTPVDSPDDSTLSESANQVFLGFTYVAPSVLESV 422
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Human
Match: RPS6KB2 (ribosomal protein S6 kinase B2 [Source:HGNC Symbol;Acc:HGNC:10437]) HSP 1 Score: 402.134 bits (1032), Expect = 2.678e-133 Identity = 193/357 (54.06%), Postives = 262/357 (73.39%), Query Frame = 2
Query: 215 ESVEICQSVVNPGV-KISMEDFKVLNVIGRGGYGKVFLVQKSSSGHDENKLYAMKVLKKARIIRNEKDTAHTKSERNILEMIRHPFLVKLHYAFQNQSNLYIVLTYCSGGELFTYLEREGTLMENQACFYVAEISLAIGHLHKTGIIYRDLKPENVLLDKKGHVKLTDFGLSKESV---SLTNTFCGTIEYMAPEILKRGGHGKEVDWWSLGTLLFDMLSGGPPFSQECDKQETITKILHSTIAYPKYFSSEVVRLIQGLLKRNPKERL-GCKNDVEDIKNHEFFKTHDINWSDVYHRRMDPPFKPELKSEMDVSCFDSTFTGQQPIMTPDDCNGAHILPDLFQGFSYVAPNVASSV 1270
E VE+ ++ VN G +I F++L V+G+GGYGKVF V+K G + K+YAMKVL+KA+I+RN KDTAHT++ERNILE ++HPF+V+L YAFQ LY++L SGGELFT+LEREG +E+ ACFY+AEI+LA+GHLH GIIYRDLKPEN++L +GH+KLTDFGL KES+ ++T+TFCGTIEYMAPEIL R GH + VDWWSLG L++DML+G PPF+ E ++++T+ KI+ +A P Y + + L++ LKRNP +R+ G D D++ H FF+ +NW D+ R+DPPF+P L+SE DVS FD+ FT Q P+ +PDD + F GF+YVAP+V S+
Sbjct: 46 EEVELTETSVNVGPERIGPHCFELLRVLGKGGYGKVFQVRKVQ-GTNLGKIYAMKVLRKAKIVRNAKDTAHTRAERNILESVKHPFIVELAYAFQTGGKLYLILECLSGGELFTHLEREGIFLEDTACFYLAEITLALGHLHSQGIIYRDLKPENIMLSSQGHIKLTDFGLCKESIHEGAVTHTFCGTIEYMAPEILVRSGHNRAVDWWSLGALMYDMLTGSPPFTAE-NRKKTMDKIIRGKLALPPYLTPDARDLVKKFLKRNPSQRIGGGPGDAADVQRHPFFR--HMNWDDLLAWRVDPPFRPCLQSEEDVSQFDTRFTRQTPVDSPDDTALSESANQAFLGFTYVAPSVLDSI 398
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Human
Match: RPS6KB1 (ribosomal protein S6 kinase B1 [Source:HGNC Symbol;Acc:HGNC:10436]) HSP 1 Score: 372.474 bits (955), Expect = 2.260e-121 Identity = 189/357 (52.94%), Postives = 244/357 (68.35%), Query Frame = 2
Query: 215 ESVEICQSVVNPGV-KISMEDFKVLNVIGRGGYGKVFLVQKSSSGHDENKLYAMKVLKKARIIRNEKDTAHTKSERNILEMIRHPFLVKLHYAFQNQSNLYIVLTYCSGGELFTYLEREGTLMENQACFYVAEISLAIGHLHKTGIIYRDLKPENVLLDKKGHVKLTDFGLSKESV---SLTNTFCGTIEYMAPEILKRGGHGKEVDWWSLGTLLFDMLSGGPPFSQECDKQETITKILHSTIAYPKYFSSEVVRLIQGLLKRNPKERLGC-KNDVEDIKNHEFFKTHDINWSDVYHRRMDPPFKPELKSEMDVSCFDSTFTGQQPIMTPDDCNGAHILPDLFQGFSYVAPNVASSV 1270
E EI ++ VN G KI E F++L V+G+GGYGK A I+RN KDTAHTK+ERNILE ++HPF+V L YAFQ LY++L Y SGGELF LEREG ME+ ACFY+AEIS+A+GHLH+ GIIYRDLKPEN++L+ +GHVKLTDFGL KES+ ++T+TFCGTIEYMAPEIL R GH + VDWWSLG L++DML+G PPF+ E ++++TI KIL + P Y + E L++ LLKRN RLG D +++ H FF+ INW ++ R+++PPFKP L+SE DVS FDS FT Q P+ +PDD + +F GF+YVAP+V SV
Sbjct: 70 EKFEISETSVNRGPEKIRPECFELLRVLGKGGYGK------------------------AMIVRNAKDTAHTKAERNILEEVKHPFIVDLIYAFQTGGKLYLILEYLSGGELFMQLEREGIFMEDTACFYLAEISMALGHLHQKGIIYRDLKPENIMLNHQGHVKLTDFGLCKESIHDGTVTHTFCGTIEYMAPEILMRSGHNRAVDWWSLGALMYDMLTGAPPFTGE-NRKKTIDKILKCKLNLPPYLTQEARDLLKKLLKRNAASRLGAGPGDAGEVQAHPFFR--HINWEELLARKVEPPFKPLLQSEEDVSQFDSKFTRQTPVDSPDDSTLSESANQVFLGFTYVAPSVLESV 399
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Celegans
Match: rsks-1 (Ribosomal protein S6 kinase beta [Source:UniProtKB/Swiss-Prot;Acc:Q9NAH6]) HSP 1 Score: 403.675 bits (1036), Expect = 2.642e-133 Identity = 199/358 (55.59%), Postives = 265/358 (74.02%), Query Frame = 2
Query: 200 EDDDIESVEICQSVVNP-GVKISMEDFKVLNVIGRGGYGKVFLVQKSSSGHDENKLYAMKVLKKARIIRNEKDTAHTKSERNILEMIRHPFLVKLHYAFQNQSNLYIVLTYCSGGELFTYLEREGTLMENQACFYVAEISLAIGHLHKTGIIYRDLKPENVLLDKKGHVKLTDFGLSKESV---SLTNTFCGTIEYMAPEILKRGGHGKEVDWWSLGTLLFDMLSGGPPFSQECDKQETITKILHSTIAYPKYFSSEVVRLIQGLLKRNPKERLGCK-NDVEDIKNHEFFKTHDINWSDVYHRRMDPPFKPELKSEMDVSCFDSTFTGQQPIMTPDDCNGAHILPDLFQGFSYVAPNV 1258
ED +ES+++C S +NP V++ EDF++L V+G+GGYGKVF V+K++ G D +++AMKVL+KA I+RN+KDTAHTK+ERNILE ++ PF+ L YAFQ LY++L Y SGGELF +LEREG MEN A FY++EI +++ HLH+ GIIYRDLKPEN+LLD GHVKLTDFGL KE + T+TFCGTIEYMAPEIL R GHGK VDWWSLG L+FDML+GGPPF+ E ++++TI KIL + P Y S+E LI+ LLKR+ RLG +D E+IK+H FFKT D W+ VY R+++ PFKP ++++ D S FD+ FT P+ +P + N + + F GF+YVAP+V
Sbjct: 57 EDPMMESIQLCASAINPPNVRVGPEDFQLLKVLGKGGYGKVFQVRKTT-GSDNGQIFAMKVLQKATIVRNQKDTAHTKAERNILEAVKSPFICDLLYAFQTGGKLYLILEYLSGGELFMHLEREGMFMENVAKFYLSEIVVSLEHLHQQGIIYRDLKPENILLDAYGHVKLTDFGLCKEEIEGDQKTHTFCGTIEYMAPEILMRCGHGKAVDWWSLGALMFDMLTGGPPFTAE-NRRKTIDKILKGRLTLPAYLSNEARDLIKKLLKRHVDTRLGAGLSDAEEIKSHAFFKTTD--WNLVYARQLEAPFKPNIENDEDTSLFDARFTKMTPVDSPCETNFSLNGDNPFVGFTYVAPSV 410
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Celegans
Match: rskn-1 (Putative ribosomal protein S6 kinase alpha-1 [Source:UniProtKB/Swiss-Prot;Acc:Q21734]) HSP 1 Score: 315.849 bits (808), Expect = 1.734e-97 Identity = 172/375 (45.87%), Postives = 236/375 (62.93%), Query Frame = 2
Query: 161 IQQFIQNGPLKREEDDDIES-VEI-CQSVVNPGVKISMEDFKVLNVIGRGGYGKVFLVQKSSSGHDENKLYAMKVLKKARIIRNEKDTAHTKSERNILEMIRHPFLVKLHYAFQNQSNLYIVLTYCSGGELFTYLEREGTLMENQACFYVAEISLAIGHLHKTGIIYRDLKPENVLLDKKGHVKLTDFGLSKESV---SLTNTFCGTIEYMAPEILKRGGHGKEVDWWSLGTLLFDMLSGGPPFSQECDKQETITKILHSTIAYPKYFSSEVVRLIQGLLKRNPKERLGCKND-VEDIKNHEFFKTHDINWSDVYHRRMDPPFKPELKSEMDVSCFDSTFTGQQPI---MTPDDCNGAHILPDLFQGFSYVAPNV 1258
+++F +N + EE D S EI V G K F++L V+G+G +GKVFLV+K G D +YAMKVLKKA + +D TK ERNIL I HPF+VKLHYAFQ + LY++L + GG+LFT L +E E+ FY+AE++LA+ HLH GI+YRDLKPEN+LLD GH+K+TDFGLSKE++ T +FCGT+EYMAPE++ R GH D+WSLG L+F+ML+G PF Q D+ +T+T+IL + ++ P + + E L++ L KRN + RLG D VE+IK H FF I++ + ++ +DPPFKP L + S FD FT + P P NG I F+GFS+V+ V
Sbjct: 7 VEEFAENEIEQGEEKTDSSSETEIDIGDVRKCGEKADPRQFELLKVLGQGSFGKVFLVRKVR-GRDSGHVYAMKVLKKATL--KVRDRQRTKLERNILAHISHPFIVKLHYAFQTEGKLYLILDFLRGGDLFTRLSKEVMFTEDDVKFYLAELTLALEHLHSLGIVYRDLKPENILLDADGHIKVTDFGLSKEAIDSEKKTYSFCGTVEYMAPEVINRRGHSMAADFWSLGVLMFEMLTGHLPF-QGRDRNDTMTQILKAKLSMPHFLTQEAQSLLRALFKRNSQNRLGAGPDGVEEIKRHAFFAK--IDFVKLLNKEIDPPFKPALSTVDSTSYFDPEFTKRTPKDSPALPASANGHEI----FRGFSFVSNAV 371 HSP 2 Score: 109.768 bits (273), Expect = 1.090e-24 Identity = 75/270 (27.78%), Postives = 137/270 (50.74%), Query Frame = 2
Query: 269 EDFKVLNVIGRGGYGKVFLVQKSSSGHDENKLYAMKVLKKARIIRNEKDTAHTKSERNILEMIRHPFLVKLHYAFQNQSNLYIVLTYCSGGELFTYLEREGTL-MENQACFYVAEISLAIGHLHKTGIIYRDLKPENVLLDKK----GHVKLTDFGLSKESVS---LTNTFCGTIEYMAPEILKRGGHGKEVDWWSLGTLLFDMLSGGPPFSQECDKQETITKILHS------TIAYPKY--FSSEVVRLIQGLLKRNPKERLGCKNDVE 1030
+D+++L IG G + V Q ++ + YA+K++KKA E+ +L H F+VKL +++++ +Y++ C GGEL L + +L E + +A + A+ +LH + +RDL N+L K +++ DFG +K+S + + T C T +++APE+L++ G+ + D WSLG LL ML+G PF+ +T +IL ++ +P + S E L++ +L +P R+ K ++
Sbjct: 395 DDYEILEKIGNGAHSVVHKCQMKAT----RRKYAVKIVKKAVFDATEEVDI-------LLRHSHHQFVVKLFDVYEDETAIYMIEELCEGGELLDKLVNKKSLGSEKEVAAIMANLLNAVQYLHSQQVAHRDLTAANILFALKDGDPSSLRIVDFGFAKQSRAENGMLMTPCYTAQFVAPEVLRKQGYDRSCDVWSLGVLLHTMLTGCTPFA--MGPNDTPDQILQRVGDGKISMTHPVWDTISDEAKDLVRKMLDVDPNRRVTAKQALQ 651
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Celegans
Match: rskn-1 (Putative ribosomal protein S6 kinase alpha-1 [Source:UniProtKB/Swiss-Prot;Acc:Q21734]) HSP 1 Score: 315.464 bits (807), Expect = 4.333e-97 Identity = 167/361 (46.26%), Postives = 228/361 (63.16%), Query Frame = 2
Query: 197 EEDDDIESVEICQSVVNPGVKISMEDFKVLNVIGRGGYGKVFLVQKSSSGHDENKLYAMKVLKKARIIRNEKDTAHTKSERNILEMIRHPFLVKLHYAFQNQSNLYIVLTYCSGGELFTYLEREGTLMENQACFYVAEISLAIGHLHKTGIIYRDLKPENVLLDKKGHVKLTDFGLSKESV---SLTNTFCGTIEYMAPEILKRGGHGKEVDWWSLGTLLFDMLSGGPPFSQECDKQETITKILHSTIAYPKYFSSEVVRLIQGLLKRNPKERLGCKND-VEDIKNHEFFKTHDINWSDVYHRRMDPPFKPELKSEMDVSCFDSTFTGQQPI---MTPDDCNGAHILPDLFQGFSYVAPNV 1258
E + DI V C +P F++L V+G+G +GKVFLV+K G D +YAMKVLKKA + +D TK ERNIL I HPF+VKLHYAFQ + LY++L + GG+LFT L +E E+ FY+AE++LA+ HLH GI+YRDLKPEN+LLD GH+K+TDFGLSKE++ T +FCGT+EYMAPE++ R GH D+WSLG L+F+ML+G PF Q D+ +T+T+IL + ++ P + + E L++ L KRN + RLG D VE+IK H FF I++ + ++ +DPPFKP L + S FD FT + P P NG I F+GFS+V+ V
Sbjct: 45 ETEIDIGDVRKCGEKADP------RQFELLKVLGQGSFGKVFLVRKVR-GRDSGHVYAMKVLKKATL--KVRDRQRTKLERNILAHISHPFIVKLHYAFQTEGKLYLILDFLRGGDLFTRLSKEVMFTEDDVKFYLAELTLALEHLHSLGIVYRDLKPENILLDADGHIKVTDFGLSKEAIDSEKKTYSFCGTVEYMAPEVINRRGHSMAADFWSLGVLMFEMLTGHLPF-QGRDRNDTMTQILKAKLSMPHFLTQEAQSLLRALFKRNSQNRLGAGPDGVEEIKRHAFFAK--IDFVKLLNKEIDPPFKPALSTVDSTSYFDPEFTKRTPKDSPALPASANGHEI----FRGFSFVSNAV 389 HSP 2 Score: 110.153 bits (274), Expect = 8.605e-25 Identity = 75/270 (27.78%), Postives = 137/270 (50.74%), Query Frame = 2
Query: 269 EDFKVLNVIGRGGYGKVFLVQKSSSGHDENKLYAMKVLKKARIIRNEKDTAHTKSERNILEMIRHPFLVKLHYAFQNQSNLYIVLTYCSGGELFTYLEREGTL-MENQACFYVAEISLAIGHLHKTGIIYRDLKPENVLLDKK----GHVKLTDFGLSKESVS---LTNTFCGTIEYMAPEILKRGGHGKEVDWWSLGTLLFDMLSGGPPFSQECDKQETITKILHS------TIAYPKY--FSSEVVRLIQGLLKRNPKERLGCKNDVE 1030
+D+++L IG G + V Q ++ + YA+K++KKA E+ +L H F+VKL +++++ +Y++ C GGEL L + +L E + +A + A+ +LH + +RDL N+L K +++ DFG +K+S + + T C T +++APE+L++ G+ + D WSLG LL ML+G PF+ +T +IL ++ +P + S E L++ +L +P R+ K ++
Sbjct: 413 DDYEILEKIGNGAHSVVHKCQMKAT----RRKYAVKIVKKAVFDATEEVDI-------LLRHSHHQFVVKLFDVYEDETAIYMIEELCEGGELLDKLVNKKSLGSEKEVAAIMANLLNAVQYLHSQQVAHRDLTAANILFALKDGDPSSLRIVDFGFAKQSRAENGMLMTPCYTAQFVAPEVLRKQGYDRSCDVWSLGVLLHTMLTGCTPFA--MGPNDTPDQILQRVGDGKISMTHPVWDTISDEAKDLVRKMLDVDPNRRVTAKQALQ 669
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Celegans
Match: rskn-1 (Putative ribosomal protein S6 kinase alpha-1 [Source:UniProtKB/Swiss-Prot;Acc:Q21734]) HSP 1 Score: 313.923 bits (803), Expect = 1.025e-96 Identity = 167/361 (46.26%), Postives = 228/361 (63.16%), Query Frame = 2
Query: 197 EEDDDIESVEICQSVVNPGVKISMEDFKVLNVIGRGGYGKVFLVQKSSSGHDENKLYAMKVLKKARIIRNEKDTAHTKSERNILEMIRHPFLVKLHYAFQNQSNLYIVLTYCSGGELFTYLEREGTLMENQACFYVAEISLAIGHLHKTGIIYRDLKPENVLLDKKGHVKLTDFGLSKESV---SLTNTFCGTIEYMAPEILKRGGHGKEVDWWSLGTLLFDMLSGGPPFSQECDKQETITKILHSTIAYPKYFSSEVVRLIQGLLKRNPKERLGCKND-VEDIKNHEFFKTHDINWSDVYHRRMDPPFKPELKSEMDVSCFDSTFTGQQPI---MTPDDCNGAHILPDLFQGFSYVAPNV 1258
E + DI V C +P F++L V+G+G +GKVFLV+K G D +YAMKVLKKA + +D TK ERNIL I HPF+VKLHYAFQ + LY++L + GG+LFT L +E E+ FY+AE++LA+ HLH GI+YRDLKPEN+LLD GH+K+TDFGLSKE++ T +FCGT+EYMAPE++ R GH D+WSLG L+F+ML+G PF Q D+ +T+T+IL + ++ P + + E L++ L KRN + RLG D VE+IK H FF I++ + ++ +DPPFKP L + S FD FT + P P NG I F+GFS+V+ V
Sbjct: 65 ETEIDIGDVRKCGEKADP------RQFELLKVLGQGSFGKVFLVRKVR-GRDSGHVYAMKVLKKATL--KVRDRQRTKLERNILAHISHPFIVKLHYAFQTEGKLYLILDFLRGGDLFTRLSKEVMFTEDDVKFYLAELTLALEHLHSLGIVYRDLKPENILLDADGHIKVTDFGLSKEAIDSEKKTYSFCGTVEYMAPEVINRRGHSMAADFWSLGVLMFEMLTGHLPF-QGRDRNDTMTQILKAKLSMPHFLTQEAQSLLRALFKRNSQNRLGAGPDGVEEIKRHAFFAK--IDFVKLLNKEIDPPFKPALSTVDSTSYFDPEFTKRTPKDSPALPASANGHEI----FRGFSFVSNAV 409 HSP 2 Score: 106.686 bits (265), Expect = 1.063e-23 Identity = 64/212 (30.19%), Postives = 113/212 (53.30%), Query Frame = 2
Query: 269 EDFKVLNVIGRGGYGKVFLVQKSSSGHDENKLYAMKVLKKARIIRNEKDTAHTKSERNILEMIRHPFLVKLHYAFQNQSNLYIVLTYCSGGELFTYLEREGTL-MENQACFYVAEISLAIGHLHKTGIIYRDLKPENVLLDKK----GHVKLTDFGLSKESVS---LTNTFCGTIEYMAPEILKRGGHGKEVDWWSLGTLLFDMLSGGPPFS 880
+D+++L IG G + V Q ++ + YA+K++KKA E+ +L H F+VKL +++++ +Y++ C GGEL L + +L E + +A + A+ +LH + +RDL N+L K +++ DFG +K+S + + T C T +++APE+L++ G+ + D WSLG LL ML+G PF+
Sbjct: 433 DDYEILEKIGNGAHSVVHKCQMKAT----RRKYAVKIVKKAVFDATEEVDI-------LLRHSHHQFVVKLFDVYEDETAIYMIEELCEGGELLDKLVNKKSLGSEKEVAAIMANLLNAVQYLHSQQVAHRDLTAANILFALKDGDPSSLRIVDFGFAKQSRAENGMLMTPCYTAQFVAPEVLRKQGYDRSCDVWSLGVLLHTMLTGCTPFA 633
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Celegans
Match: rskn-1 (Putative ribosomal protein S6 kinase alpha-1 [Source:UniProtKB/Swiss-Prot;Acc:Q21734]) HSP 1 Score: 314.309 bits (804), Expect = 2.295e-96 Identity = 167/361 (46.26%), Postives = 228/361 (63.16%), Query Frame = 2
Query: 197 EEDDDIESVEICQSVVNPGVKISMEDFKVLNVIGRGGYGKVFLVQKSSSGHDENKLYAMKVLKKARIIRNEKDTAHTKSERNILEMIRHPFLVKLHYAFQNQSNLYIVLTYCSGGELFTYLEREGTLMENQACFYVAEISLAIGHLHKTGIIYRDLKPENVLLDKKGHVKLTDFGLSKESV---SLTNTFCGTIEYMAPEILKRGGHGKEVDWWSLGTLLFDMLSGGPPFSQECDKQETITKILHSTIAYPKYFSSEVVRLIQGLLKRNPKERLGCKND-VEDIKNHEFFKTHDINWSDVYHRRMDPPFKPELKSEMDVSCFDSTFTGQQPI---MTPDDCNGAHILPDLFQGFSYVAPNV 1258
E + DI V C +P F++L V+G+G +GKVFLV+K G D +YAMKVLKKA + +D TK ERNIL I HPF+VKLHYAFQ + LY++L + GG+LFT L +E E+ FY+AE++LA+ HLH GI+YRDLKPEN+LLD GH+K+TDFGLSKE++ T +FCGT+EYMAPE++ R GH D+WSLG L+F+ML+G PF Q D+ +T+T+IL + ++ P + + E L++ L KRN + RLG D VE+IK H FF I++ + ++ +DPPFKP L + S FD FT + P P NG I F+GFS+V+ V
Sbjct: 84 ETEIDIGDVRKCGEKADP------RQFELLKVLGQGSFGKVFLVRKVR-GRDSGHVYAMKVLKKATL--KVRDRQRTKLERNILAHISHPFIVKLHYAFQTEGKLYLILDFLRGGDLFTRLSKEVMFTEDDVKFYLAELTLALEHLHSLGIVYRDLKPENILLDADGHIKVTDFGLSKEAIDSEKKTYSFCGTVEYMAPEVINRRGHSMAADFWSLGVLMFEMLTGHLPF-QGRDRNDTMTQILKAKLSMPHFLTQEAQSLLRALFKRNSQNRLGAGPDGVEEIKRHAFFAK--IDFVKLLNKEIDPPFKPALSTVDSTSYFDPEFTKRTPKDSPALPASANGHEI----FRGFSFVSNAV 428 HSP 2 Score: 110.923 bits (276), Expect = 6.320e-25 Identity = 75/270 (27.78%), Postives = 137/270 (50.74%), Query Frame = 2
Query: 269 EDFKVLNVIGRGGYGKVFLVQKSSSGHDENKLYAMKVLKKARIIRNEKDTAHTKSERNILEMIRHPFLVKLHYAFQNQSNLYIVLTYCSGGELFTYLEREGTL-MENQACFYVAEISLAIGHLHKTGIIYRDLKPENVLLDKK----GHVKLTDFGLSKESVS---LTNTFCGTIEYMAPEILKRGGHGKEVDWWSLGTLLFDMLSGGPPFSQECDKQETITKILHS------TIAYPKY--FSSEVVRLIQGLLKRNPKERLGCKNDVE 1030
+D+++L IG G + V Q ++ + YA+K++KKA E+ +L H F+VKL +++++ +Y++ C GGEL L + +L E + +A + A+ +LH + +RDL N+L K +++ DFG +K+S + + T C T +++APE+L++ G+ + D WSLG LL ML+G PF+ +T +IL ++ +P + S E L++ +L +P R+ K ++
Sbjct: 452 DDYEILEKIGNGAHSVVHKCQMKAT----RRKYAVKIVKKAVFDATEEVDI-------LLRHSHHQFVVKLFDVYEDETAIYMIEELCEGGELLDKLVNKKSLGSEKEVAAIMANLLNAVQYLHSQQVAHRDLTAANILFALKDGDPSSLRIVDFGFAKQSRAENGMLMTPCYTAQFVAPEVLRKQGYDRSCDVWSLGVLLHTMLTGCTPFA--MGPNDTPDQILQRVGDGKISMTHPVWDTISDEAKDLVRKMLDVDPNRRVTAKQALQ 708
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Fly
Match: S6k (gene:FBgn0283472 transcript:FBtr0077114) HSP 1 Score: 414.846 bits (1065), Expect = 2.209e-138 Identity = 198/353 (56.09%), Postives = 263/353 (74.50%), Query Frame = 2
Query: 215 ESVEICQSVVNPG-VKISMEDFKVLNVIGRGGYGKVFLVQKSSSGHDENKLYAMKVLKKARIIRNEKDTAHTKSERNILEMIRHPFLVKLHYAFQNQSNLYIVLTYCSGGELFTYLEREGTLMENQACFYVAEISLAIGHLHKTGIIYRDLKPENVLLDKKGHVKLTDFGLSKESVS---LTNTFCGTIEYMAPEILKRGGHGKEVDWWSLGTLLFDMLSGGPPFSQECDKQETITKILHSTIAYPKYFSSEVVRLIQGLLKRNPKERLGC-KNDVEDIKNHEFFKTHDINWSDVYHRRMDPPFKPELKSEMDVSCFDSTFTGQQPIMTPDDCNGAHILPDLFQGFSYVAPNV 1258
E++++C+ VNPG +K+ +DF++ V+G+GGYGKVF V+K++ G D NK +AMKVLKKA I+ N+KDTAHT++ERNILE ++HPF+V+L YAFQ LY++L Y SGGELF +LEREG +E+ CFY++EI LA+GHLHK GIIYRDLKPEN+LLD +GHVKLTDFGL KE + +T+TFCGTIEYMAPEIL R GHGK VDWWSLG L+FDML+G PPF+ E ++++TI IL + + P Y + E L++ L+KR +RLG D ++ H FFK +NW DV RR++PP KP L+SE DVS FD+ FT Q P+ +PDD + +FQGF+YVAP++
Sbjct: 56 ETIQLCEENVNPGKIKLGPKDFELKKVLGKGGYGKVFQVRKTA-GRDANKYFAMKVLKKASIVTNQKDTAHTRAERNILEAVKHPFIVELVYAFQTDGKLYLILEYLSGGELFMHLEREGIFLEDTTCFYLSEIILALGHLHKLGIIYRDLKPENILLDAQGHVKLTDFGLCKEHIQEGIVTHTFCGTIEYMAPEILTRSGHGKAVDWWSLGALMFDMLTGVPPFTAE-NRKKTIETILKAKLNLPAYLTPEARDLVRRLMKRQEPQRLGSGPEDAAAVQIHPFFK--HVNWDDVLARRLEPPIKPLLRSEDDVSQFDTRFTRQIPVDSPDDTTLSESANLIFQGFTYVAPSI 404
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Fly
Match: S6k (gene:FBgn0283472 transcript:FBtr0333264) HSP 1 Score: 414.846 bits (1065), Expect = 2.209e-138 Identity = 198/353 (56.09%), Postives = 263/353 (74.50%), Query Frame = 2
Query: 215 ESVEICQSVVNPG-VKISMEDFKVLNVIGRGGYGKVFLVQKSSSGHDENKLYAMKVLKKARIIRNEKDTAHTKSERNILEMIRHPFLVKLHYAFQNQSNLYIVLTYCSGGELFTYLEREGTLMENQACFYVAEISLAIGHLHKTGIIYRDLKPENVLLDKKGHVKLTDFGLSKESVS---LTNTFCGTIEYMAPEILKRGGHGKEVDWWSLGTLLFDMLSGGPPFSQECDKQETITKILHSTIAYPKYFSSEVVRLIQGLLKRNPKERLGC-KNDVEDIKNHEFFKTHDINWSDVYHRRMDPPFKPELKSEMDVSCFDSTFTGQQPIMTPDDCNGAHILPDLFQGFSYVAPNV 1258
E++++C+ VNPG +K+ +DF++ V+G+GGYGKVF V+K++ G D NK +AMKVLKKA I+ N+KDTAHT++ERNILE ++HPF+V+L YAFQ LY++L Y SGGELF +LEREG +E+ CFY++EI LA+GHLHK GIIYRDLKPEN+LLD +GHVKLTDFGL KE + +T+TFCGTIEYMAPEIL R GHGK VDWWSLG L+FDML+G PPF+ E ++++TI IL + + P Y + E L++ L+KR +RLG D ++ H FFK +NW DV RR++PP KP L+SE DVS FD+ FT Q P+ +PDD + +FQGF+YVAP++
Sbjct: 56 ETIQLCEENVNPGKIKLGPKDFELKKVLGKGGYGKVFQVRKTA-GRDANKYFAMKVLKKASIVTNQKDTAHTRAERNILEAVKHPFIVELVYAFQTDGKLYLILEYLSGGELFMHLEREGIFLEDTTCFYLSEIILALGHLHKLGIIYRDLKPENILLDAQGHVKLTDFGLCKEHIQEGIVTHTFCGTIEYMAPEILTRSGHGKAVDWWSLGALMFDMLTGVPPFTAE-NRKKTIETILKAKLNLPAYLTPEARDLVRRLMKRQEPQRLGSGPEDAAAVQIHPFFK--HVNWDDVLARRLEPPIKPLLRSEDDVSQFDTRFTRQIPVDSPDDTTLSESANLIFQGFTYVAPSI 404
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Fly
Match: Pkc98E (gene:FBgn0003093 transcript:FBtr0301516) HSP 1 Score: 286.574 bits (732), Expect = 4.304e-88 Identity = 145/340 (42.65%), Postives = 215/340 (63.24%), Query Frame = 2
Query: 248 PGVKISMEDFKVLNVIGRGGYGKVFLVQKSSSGHDENKLYAMKVLKKARIIRNEKDTAHTKSERNILEMI-RHPFLVKLHYAFQNQSNLYIVLTYCSGGELFTYLEREGTLMENQACFYVAEISLAIGHLHKTGIIYRDLKPENVLLDKKGHVKLTDFGLSKESV---SLTNTFCGTIEYMAPEILKRGGHGKEVDWWSLGTLLFDMLSGGPPFSQECDKQETITKILHSTIAYPKYFSSEVVRLIQGLLKRNPKERLGCKNDVEDIKNHEFFKTHDINWSDVYHRRMDPPFKPELKSEMDVSCFDSTFTGQQPIMTPDDCNGAHIL-PDLFQGFSYVAP 1252
PG K S+ DF + V+G+G +GKV L +K G DE +YA+KVLKK II+++ D T +E+ IL + HPFL LH FQ L+ V+ Y +GG+L +++ ++A FY AE++LA+ LH G+IYRDLK +N+LLD++GH KL DFG+ KE + LT TFCGT +Y+APEILK +G VDWW+LG L+++M++G PPF + ++ E I+H + YP + S E V +++G L +NP++RLGC D +I+ H FF D W ++ R + PPF+P++K+ D + FD+ FT + P++TP + D F GFS+V P
Sbjct: 215 PG-KCSLLDFNFIKVLGKGSFGKVMLAEKK--GTDE--IYAIKVLKKDAIIQDD-DVDCTMTEKRILALAANHPFLTALHSCFQTPDRLFFVMEYVNGGDLMFQIQKARRFEASRAAFYAAEVTLALQFLHTHGVIYRDLKLDNILLDQEGHCKLADFGMCKEGIMNGMLTTTFCGTPDYIAPEILKEQEYGASVDWWALGVLMYEMMAGQPPFEAD-NEDELFDSIMHDDVLYPVWLSREAVSILKGFLTKNPEQRLGCTGDENEIRKHPFFAKLD--WKELEKRNIKPPFRPKMKNPRDANNFDAEFTKEDPVLTPIGNEVVRCINQDEFAGFSFVNP 545
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Fly
Match: S6kII (gene:FBgn0262866 transcript:FBtr0333715) HSP 1 Score: 295.434 bits (755), Expect = 4.338e-88 Identity = 157/331 (47.43%), Postives = 212/331 (64.05%), Query Frame = 2
Query: 275 FKVLNVIGRGGYGKVFLVQKSSSGHDENKLYAMKVLKKARIIRNEKDTAHTKSERNILEMIRHPFLVKLHYAFQNQSNLYIVLTYCSGGELFTYLEREGTLMENQACFYVAEISLAIGHLHKTGIIYRDLKPENVLLDKKGHVKLTDFGLSKESV--SLTNTFCGTIEYMAPEILKRGGHGKEVDWWSLGTLLFDMLSGGPPFSQECDKQETITKILHSTIAYPKYFSSEVVRLIQGLLKRNPKERLGC-KNDVEDIKNHEFFKTHDINWSDVYHRRMDPPFKPELKSEMDVSCFDSTFTGQQPIMTPDD--CNGAHILPDLFQGFSYVAP 1252
F++L V+G G +GKVFLV+K G D LYAMKVLKKA + KD + +ER IL + H F+V+LHYAFQ LY++L + GG+LFT L +E E FY+AE++LA+ HLH GIIYRDLKPEN+LLD+ GH+ LTDFGLSK+ + S T +FCGT+EYMAPEI+ R GH DWWS G L+++ML+G PF + +QET+ +IL S + P+ S E L++ L KRNP+ RLG + DIK H FF T I+W + +++ PPF P + S D FD +T + P +P AH ++F+GFS+VAP
Sbjct: 199 FELLRVLGEGSFGKVFLVRKII-GKDAGTLYAMKVLKKATL--KVKDRVRSTNERKILADVGHAFIVRLHYAFQTPGKLYLILDFLRGGDLFTRLSKEVMFTEEDVKFYLAELALAMNHLHTLGIIYRDLKPENILLDEHGHIALTDFGLSKQPLDGSKTYSFCGTVEYMAPEIVNRKGHDFAADWWSFGVLMYEMLTGNLPFHGQT-RQETMNQILRSKLGMPENLSPEAQSLLRALFKRNPQNRLGAGAQGILDIKAHCFFAT--IDWVRLERKQVRPPFIPAV-SRDDAFYFDVEYTSKSPRDSPGGPISASAH---EIFRGFSFVAP 519 HSP 2 Score: 108.612 bits (270), Expect = 5.044e-24 Identity = 70/213 (32.86%), Postives = 116/213 (54.46%), Query Frame = 2
Query: 272 DFKVLNVIGRGGYGKVFLVQKSSSGHDENKLYAMKVLKKARIIRNEKDTAHTKSERNILEMIR---HPFLVKLHYAFQNQSNLYIVLTYCSGGELFTYLEREGTLMENQACFYVAEISLAIGHLHKTGIIYRDLKPENVLLDKKGH----VKLTDFGLSKESVS---LTNTFCGTIEYMAPEILKRGGHGKEVDWWSLGTLLFDMLSGGPPFS 880
++ +L +GRG + L + +S K YA+KV++KA + T+ E + M+R HP +V L+ +++ + Y+V+ GGEL + G + E++A + I+ A+ +LH+ G+++RDLKP N++ +KL D G +K+ + L T C T ++APE+LKR G+ D WSLG LL+ MLSG PF+
Sbjct: 567 EYNLLQELGRGTFSVCRLCEHRAS----KKHYAVKVIEKAAVAAASTSTSADCWE-EVEIMLRYGNHPNIVTLYSVYEDAGSAYLVMELLKGGELLDRILAVGQMCESEASAVLRTIASAVAYLHEHGVVHRDLKPSNMIYASMRQTPETLKLCDLGFAKQLRADNGLLMTPCYTANFVAPEVLKRQGYDLACDIWSLGVLLYIMLSGRTPFA 774
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Fly
Match: S6kII (gene:FBgn0262866 transcript:FBtr0077360) HSP 1 Score: 295.434 bits (755), Expect = 4.338e-88 Identity = 157/331 (47.43%), Postives = 212/331 (64.05%), Query Frame = 2
Query: 275 FKVLNVIGRGGYGKVFLVQKSSSGHDENKLYAMKVLKKARIIRNEKDTAHTKSERNILEMIRHPFLVKLHYAFQNQSNLYIVLTYCSGGELFTYLEREGTLMENQACFYVAEISLAIGHLHKTGIIYRDLKPENVLLDKKGHVKLTDFGLSKESV--SLTNTFCGTIEYMAPEILKRGGHGKEVDWWSLGTLLFDMLSGGPPFSQECDKQETITKILHSTIAYPKYFSSEVVRLIQGLLKRNPKERLGC-KNDVEDIKNHEFFKTHDINWSDVYHRRMDPPFKPELKSEMDVSCFDSTFTGQQPIMTPDD--CNGAHILPDLFQGFSYVAP 1252
F++L V+G G +GKVFLV+K G D LYAMKVLKKA + KD + +ER IL + H F+V+LHYAFQ LY++L + GG+LFT L +E E FY+AE++LA+ HLH GIIYRDLKPEN+LLD+ GH+ LTDFGLSK+ + S T +FCGT+EYMAPEI+ R GH DWWS G L+++ML+G PF + +QET+ +IL S + P+ S E L++ L KRNP+ RLG + DIK H FF T I+W + +++ PPF P + S D FD +T + P +P AH ++F+GFS+VAP
Sbjct: 199 FELLRVLGEGSFGKVFLVRKII-GKDAGTLYAMKVLKKATL--KVKDRVRSTNERKILADVGHAFIVRLHYAFQTPGKLYLILDFLRGGDLFTRLSKEVMFTEEDVKFYLAELALAMNHLHTLGIIYRDLKPENILLDEHGHIALTDFGLSKQPLDGSKTYSFCGTVEYMAPEIVNRKGHDFAADWWSFGVLMYEMLTGNLPFHGQT-RQETMNQILRSKLGMPENLSPEAQSLLRALFKRNPQNRLGAGAQGILDIKAHCFFAT--IDWVRLERKQVRPPFIPAV-SRDDAFYFDVEYTSKSPRDSPGGPISASAH---EIFRGFSFVAP 519 HSP 2 Score: 108.612 bits (270), Expect = 5.044e-24 Identity = 70/213 (32.86%), Postives = 116/213 (54.46%), Query Frame = 2
Query: 272 DFKVLNVIGRGGYGKVFLVQKSSSGHDENKLYAMKVLKKARIIRNEKDTAHTKSERNILEMIR---HPFLVKLHYAFQNQSNLYIVLTYCSGGELFTYLEREGTLMENQACFYVAEISLAIGHLHKTGIIYRDLKPENVLLDKKGH----VKLTDFGLSKESVS---LTNTFCGTIEYMAPEILKRGGHGKEVDWWSLGTLLFDMLSGGPPFS 880
++ +L +GRG + L + +S K YA+KV++KA + T+ E + M+R HP +V L+ +++ + Y+V+ GGEL + G + E++A + I+ A+ +LH+ G+++RDLKP N++ +KL D G +K+ + L T C T ++APE+LKR G+ D WSLG LL+ MLSG PF+
Sbjct: 567 EYNLLQELGRGTFSVCRLCEHRAS----KKHYAVKVIEKAAVAAASTSTSADCWE-EVEIMLRYGNHPNIVTLYSVYEDAGSAYLVMELLKGGELLDRILAVGQMCESEASAVLRTIASAVAYLHEHGVVHRDLKPSNMIYASMRQTPETLKLCDLGFAKQLRADNGLLMTPCYTANFVAPEVLKRQGYDLACDIWSLGVLLYIMLSGRTPFA 774
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Zebrafish
Match: rps6kb1b (ribosomal protein S6 kinase b, polypeptide 1b [Source:ZFIN;Acc:ZDB-GENE-040426-2038]) HSP 1 Score: 408.683 bits (1049), Expect = 1.218e-135 Identity = 200/360 (55.56%), Postives = 262/360 (72.78%), Query Frame = 2
Query: 206 DDIESVEICQSVVNPGVK-ISMEDFKVLNVIGRGGYGKVFLVQKSSSGHDENKLYAMKVLKKARIIRNEKDTAHTKSERNILEMIRHPFLVKLHYAFQNQSNLYIVLTYCSGGELFTYLEREGTLMENQACFYVAEISLAIGHLHKTGIIYRDLKPENVLLDKKGHVKLTDFGLSKESV---SLTNTFCGTIEYMAPEILKRGGHGKEVDWWSLGTLLFDMLSGGPPFSQECDKQETITKILHSTIAYPKYFSSEVVRLIQGLLKRNPKERLGC-KNDVEDIKNHEFFKTHDINWSDVYHRRMDPPFKPELKSEMDVSCFDSTFTGQQPIMTPDDCNGAHILPDLFQGFSYVAPNVASSV 1270
DD E +EI + VN G + I E F++L V+G+GGYGKVF V+K S G K++AMKVLKKA I+RN KDTAHTK+ERNILE ++HPF+V L YAFQ LY++L Y SGGELF LEREG ME+ ACFY+AEIS+A+GHLH+ GIIYRDLKPEN++L+ +GHVKLTDFGL KES+ ++T+TFCGTIEYMAPEIL R GH + VDWWSLG L++DML+G PPF+ E ++++TI KIL + P Y + E L++ LLKR+ RLG D +++ H FF+ +NW D+ R+++PPFKP L+S DVS FDS FT Q P+ +PDD + +F GF+YVAP+V ++
Sbjct: 41 DDCEKIEISEDNVNQGTENIRPECFELLRVLGKGGYGKVFQVRKVS-GAATGKIFAMKVLKKAMIVRNAKDTAHTKAERNILEEVKHPFIVDLIYAFQTGGKLYLILEYLSGGELFMQLEREGIFMEDTACFYLAEISMALGHLHQKGIIYRDLKPENIMLNNQGHVKLTDFGLCKESIHDGTVTHTFCGTIEYMAPEILMRSGHNRAVDWWSLGALMYDMLTGAPPFTGE-NRKKTIDKILKCKLNLPPYLTQEARDLLKRLLKRSASSRLGAGPGDATEVQTHPFFR--HVNWDDLLARKVEPPFKPFLQSAEDVSQFDSKFTSQTPVDSPDDSTLSESANQVFLGFTYVAPSVLENI 396
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Zebrafish
Match: rps6kb1a (ribosomal protein S6 kinase b, polypeptide 1a [Source:ZFIN;Acc:ZDB-GENE-091113-52]) HSP 1 Score: 404.06 bits (1037), Expect = 8.323e-134 Identity = 201/360 (55.83%), Postives = 260/360 (72.22%), Query Frame = 2
Query: 206 DDIESVEICQSVVNPGV-KISMEDFKVLNVIGRGGYGKVFLVQKSSSGHDENKLYAMKVLKKARIIRNEKDTAHTKSERNILEMIRHPFLVKLHYAFQNQSNLYIVLTYCSGGELFTYLEREGTLMENQACFYVAEISLAIGHLHKTGIIYRDLKPENVLLDKKGHVKLTDFGLSKESV---SLTNTFCGTIEYMAPEILKRGGHGKEVDWWSLGTLLFDMLSGGPPFSQECDKQETITKILHSTIAYPKYFSSEVVRLIQGLLKRNPKERLGCK-NDVEDIKNHEFFKTHDINWSDVYHRRMDPPFKPELKSEMDVSCFDSTFTGQQPIMTPDDCNGAHILPDLFQGFSYVAPNVASSV 1270
+D E EI + VN G KI E F++L V+G+GGYGKVF V+K + G K++AMKVLKKA I+RN KDTAHTK+ER+ILE ++HPF+V L YAFQ LY++L Y SGGELF LEREG +E+ ACFY+AEISLA+GHLH+ GIIYRDLKPEN++L+ GHVKLTDFGL KES+ ++T+TFCGTIEYMAPEIL R GH + VDWWSLG L++DML+G PPF+ E ++++TI KIL + P Y + E L++ LLKRN R+G DV DI+ H FF+ INW D+ ++DPPFKP L+S DVS FDS FT Q P+ +PDD + +F GF+Y+AP+V ++
Sbjct: 41 EDCEKFEISEDSVNQGTEKIRPECFELLRVLGKGGYGKVFQVRKVA-GAASGKIFAMKVLKKAMIVRNAKDTAHTKAERSILEEVKHPFIVDLIYAFQTGEKLYLILEYLSGGELFMQLEREGIFLEDTACFYLAEISLALGHLHQKGIIYRDLKPENIMLNYNGHVKLTDFGLCKESIHDGTVTHTFCGTIEYMAPEILTRSGHNRAVDWWSLGALMYDMLTGAPPFTAE-NRKKTIDKILKCKLNLPPYLTQEARDLLKKLLKRNASVRIGASPRDVLDIQRHSFFR--HINWDDLLAFKIDPPFKPFLQSADDVSQFDSKFTSQTPVDSPDDTKLSESDNQVFLGFTYIAPSVLENI 396
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Zebrafish
Match: BX000486.1 (ribosomal protein S6 kinase b, polypeptide 1a [Source:NCBI gene;Acc:562242]) HSP 1 Score: 403.675 bits (1036), Expect = 9.885e-134 Identity = 201/360 (55.83%), Postives = 260/360 (72.22%), Query Frame = 2
Query: 206 DDIESVEICQSVVNPGV-KISMEDFKVLNVIGRGGYGKVFLVQKSSSGHDENKLYAMKVLKKARIIRNEKDTAHTKSERNILEMIRHPFLVKLHYAFQNQSNLYIVLTYCSGGELFTYLEREGTLMENQACFYVAEISLAIGHLHKTGIIYRDLKPENVLLDKKGHVKLTDFGLSKESV---SLTNTFCGTIEYMAPEILKRGGHGKEVDWWSLGTLLFDMLSGGPPFSQECDKQETITKILHSTIAYPKYFSSEVVRLIQGLLKRNPKERLGCK-NDVEDIKNHEFFKTHDINWSDVYHRRMDPPFKPELKSEMDVSCFDSTFTGQQPIMTPDDCNGAHILPDLFQGFSYVAPNVASSV 1270
+D E EI + VN G KI E F++L V+G+GGYGKVF V+K + G K++AMKVLKKA I+RN KDTAHTK+ER+ILE ++HPF+V L YAFQ LY++L Y SGGELF LEREG +E+ ACFY+AEISLA+GHLH+ GIIYRDLKPEN++L+ GHVKLTDFGL KES+ ++T+TFCGTIEYMAPEIL R GH + VDWWSLG L++DML+G PPF+ E ++++TI KIL + P Y + E L++ LLKRN R+G DV DI+ H FF+ INW D+ ++DPPFKP L+S DVS FDS FT Q P+ +PDD + +F GF+Y+AP+V ++
Sbjct: 42 EDCEKFEISEDSVNQGTEKIRPECFELLRVLGKGGYGKVFQVRKVA-GAASGKIFAMKVLKKAMIVRNAKDTAHTKAERSILEEVKHPFIVDLIYAFQTGEKLYLILEYLSGGELFMQLEREGIFLEDTACFYLAEISLALGHLHQKGIIYRDLKPENIMLNYNGHVKLTDFGLCKESIHDGTVTHTFCGTIEYMAPEILTRSGHNRAVDWWSLGALMYDMLTGAPPFTAE-NRKKTIDKILKCKLNLPPYLTQEARDLLKKLLKRNASVRIGASPRDVLDIQRHSFFR--HINWDDLLAFKIDPPFKPFLQSADDVSQFDSKFTSQTPVDSPDDTKLSESDNQVFLGFTYIAPSVLENI 397
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Zebrafish
Match: rps6kb1b (ribosomal protein S6 kinase b, polypeptide 1b [Source:ZFIN;Acc:ZDB-GENE-040426-2038]) HSP 1 Score: 367.081 bits (941), Expect = 3.145e-119 Identity = 191/382 (50.00%), Postives = 257/382 (67.28%), Query Frame = 2
Query: 206 DDIESVEICQSVVNPGVK-ISMEDFKVLNVIGRGGYGKVFLVQKSSSGHDENKLYAMKVLKKARIIRNEKDTAHTKSERNILEMIRHPFLVKLHYAFQNQSNLYIVLTYCSGGEL-------FTYLER--------EGTLMENQAC-------FYVAEISLAIGHLHKTGIIYRDLKPENVLLDKKGHVKLTDFGLSKESV---SLTNTFCGTIEYMAPEILKRGGHGKEVDWWSLGTLLFDMLSGGPPFSQECDKQETITKILHSTIAYPKYFSSEVVRLIQGLLKRNPKERLGC-KNDVEDIKNHEFFKTHDINWSDVYHRRMDPPFKPELKSEMDVSCFDSTFTGQQPIMTPDDCNGAHILPDLFQGFSYVAPNVASSV 1270
DD E +EI + VN G + I E F++L V+G+GGYGKVF V+K S G K++AMKVLKKA I+RN KDTAHTK+ERNILE ++HPF+V L YAFQ LY++L Y SG L F++ +G + + C FY+AEIS+A+GHLH+ GIIYRDLKPEN++L+ +GHVKLTDFGL KES+ ++T+TFCGTIEYMAPEIL R GH + VDWWSLG L++DML+G PPF+ E ++++TI KIL + P Y + E L++ LLKR+ RLG D +++ H FF+ +NW D+ R+++PPFKP L+S DVS FDS FT Q P+ +PDD + +F GF+YVAP+V ++
Sbjct: 41 DDCEKIEISEDNVNQGTENIRPECFELLRVLGKGGYGKVFQVRKVS-GAATGKIFAMKVLKKAMIVRNAKDTAHTKAERNILEEVKHPFIVDLIYAFQTGGKLYLILEYLSGLNLCFLEIDNFSFFPHSFCCCGVAKGLIQPLKCCSGLLTCSFYLAEISMALGHLHQKGIIYRDLKPENIMLNNQGHVKLTDFGLCKESIHDGTVTHTFCGTIEYMAPEILMRSGHNRAVDWWSLGALMYDMLTGAPPFTGE-NRKKTIDKILKCKLNLPPYLTQEARDLLKRLLKRSASSRLGAGPGDATEVQTHPFFR--HVNWDDLLARKVEPPFKPFLQSAEDVSQFDSKFTSQTPVDSPDDSTLSESANQVFLGFTYVAPSVLENI 418
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Zebrafish
Match: rps6ka2 (ribosomal protein S6 kinase, polypeptide 2 [Source:ZFIN;Acc:ZDB-GENE-090827-1]) HSP 1 Score: 333.954 bits (855), Expect = 4.728e-104 Identity = 177/387 (45.74%), Postives = 244/387 (63.05%), Query Frame = 2
Query: 191 KREEDDDIESVEICQSVVNPGVKISMEDFKVLNVIGRGGYGKVFLVQKSSSGHDENKLYAMKVLKKARIIRNEKDTAHTKSERNILEMIRHPFLVKLHYAFQNQSNLYIVLTYCSGGELFTYLEREGTLMENQACFYVAEISLAIGHLHKTGIIYRDLKPENVLLDKKGHVKLTDFGLSKESV---SLTNTFCGTIEYMAPEILKRGGHGKEVDWWSLGTLLFDMLSGGPPFSQECDKQETITKILHSTIAYPKYFSSEVVRLIQGLLKRNPKERLGCKND-VEDIKNHEFFKTHDINWSDVYHRRMDPPFKPELKSEMDVSCFDSTFTGQQPIMTPDDCNGAHILPDLFQGFSYVAPNVASSVLNEPMTGVSRHTRRRNNSGLSGN 1339
K E+D ++ +I V K F++L V+G+G YGKVFLV+K G D +LYAMKVLKKA + +D +K ER+IL + HPF+VKLHYAFQ + LY++L + GG+LFT L +E E FY+AE++LA+ HLH GIIYRDLKPEN+LLD++GH+K+TDFGLSKE++ +FCGTIEYMAPE++ R GH + DWWS G L+F+ML+G PF Q D++ET+ IL + + P++ S EV L++ L KRNP RLG D VE+IK H FF T I+W+ +Y R + PPFKP + D FD FT + P +P A+ LF+GFS+VA N+ +P+T + +++ L GN
Sbjct: 31 KLEDDSILKEFDISHHVKEGFEKADPSQFELLKVLGQGSYGKVFLVRKIK-GSDTGQLYAMKVLKKATL--KVRDRVRSKMERDILAEVNHPFIVKLHYAFQTEGKLYLILDFLRGGDLFTRLSKEVMFTEEDVKFYLAELALALDHLHSLGIIYRDLKPENILLDEEGHIKITDFGLSKEAIDHDKRAYSFCGTIEYMAPEVVNRRGHTQSADWWSFGVLMFEMLTGSLPF-QGKDRKETMALILKAKLGMPQFLSPEVQSLLRALFKRNPSNRLGAGPDGVEEIKRHIFFAT--IDWNKLYRREIKPPFKPAVGRPEDTFHFDPEFTSRTPTDSPGVPASANA-HQLFRGFSFVATNLGQ---EQPLTEIKQNSVNPIVQQLHGN 407 HSP 2 Score: 104.375 bits (259), Expect = 9.692e-23 Identity = 83/282 (29.43%), Postives = 139/282 (49.29%), Query Frame = 2
Query: 224 EICQSVVNPGVK------ISMED-FKVLNVIGRGGYGKVFLVQKSSSGHDENKLYAMKVLKKARIIRNEKDTAHTKSERNILEMIRHPFLVKLHYAFQNQSNLYIVLTYCSGGELFTYLEREGTLMENQACFYVAEISLAIGHLHKTGIIYRDLKPENVL-LDKKG---HVKLTDFGLSKESVS---LTNTFCGTIEYMAPEILKRGGHGKEVDWWSLGTLLFDMLSGGPPFSQECDK--QETITKILHSTIAYP----KYFSSEVVRLIQGLLKRNPKERL 1009
EI Q+ VNP V+ I D +++ IG G Y V K + YA+K+ I R++KD ++ +L +HP ++ L + + +Y+V+ GGEL + R+ E +A + I+ + +LH G+++RDLKP N+L +D+ G +++ DFG +K+ + L T C T ++APE+LK+ G+ D WSLG LL+ ML+G PF+ D +E + +I A S ++ +L +P +RL
Sbjct: 391 EIKQNSVNPIVQQLHGNNIHFTDGYELKEDIGIGAYS----VCKRCVHRITSVEYAVKI-----IDRSKKDP--SEEIEILLRYGQHPNIITLKDVYDDGKFVYLVMELMRGGELLDRILRQKCFSEREASAVLCTITKTVEYLHSQGVVHRDLKPSNILYVDETGDPESIRICDFGFAKQLRAENGLLMTPCYTANFVAPEVLKKQGYDAACDIWSLGILLYTMLAGFTPFANGPDDTPEEILARIGSGKFALSGGNWDTVSDAAKDIVTKMLHVDPHQRL 661
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Xenopus
Match: l3hypdh (L-3-hydroxyproline dehydratase (trans-) [Source:Xenbase;Acc:XB-GENE-1000732]) HSP 1 Score: 412.535 bits (1059), Expect = 9.899e-137 Identity = 208/380 (54.74%), Postives = 271/380 (71.32%), Query Frame = 2
Query: 149 DELSIQQFIQNGPLKREED-DDIESVEICQSVVNPGV-KISMEDFKVLNVIGRGGYGKVFLVQKSSSGHDENKLYAMKVLKKARIIRNEKDTAHTKSERNILEMIRHPFLVKLHYAFQNQSNLYIVLTYCSGGELFTYLEREGTLMENQACFYVAEISLAIGHLHKTGIIYRDLKPENVLLDKKGHVKLTDFGLSKESV---SLTNTFCGTIEYMAPEILKRGGHGKEVDWWSLGTLLFDMLSGGPPFSQECDKQETITKILHSTIAYPKYFSSEVVRLIQGLLKRNPKERLGC-KNDVEDIKNHEFFKTHDINWSDVYHRRMDPPFKPELKSEMDVSCFDSTFTGQQPIMTPDDCNGAHILPDLFQGFSYVAPNVASSV 1270
D ++ +F+ +G L E + E EI ++ VN G KI E F++L V+G+GGYGKVF V+K H K++AMKVLKKA I+RN KDTAHTK+ERNILE ++HPF+V L YAFQ LY++L Y SGGELF LEREG ME+ ACFY+AEIS+A+GHLH+ GIIYRDLKPEN++L+ +GHVKLTDFGL KES+ ++T+TFCGTIEYMAPEIL R GH + VDWWSLG L++DML+G PPF+ E ++++TI KIL + P Y + E L++ LLKRN RLG D D++ H FF+ INW ++ R+++PPFKP L+SE DVS FDS FT Q P+ +PDD + +F GF+YVAP+V SV
Sbjct: 45 DGGTLTEFMDHGSLSYELGMEHCEKFEISETSVNRGPEKIRPECFELLRVLGKGGYGKVFQVRKVIGAHT-GKIFAMKVLKKAMIVRNAKDTAHTKAERNILEEVKHPFIVDLIYAFQTGGKLYLILEYLSGGELFMQLEREGIFMEDTACFYLAEISMALGHLHQKGIIYRDLKPENIMLNHQGHVKLTDFGLCKESIHDGTVTHTFCGTIEYMAPEILMRSGHNRAVDWWSLGALMYDMLTGAPPFTGE-NRKKTIDKILKCKLNLPPYLTQEARDLLKKLLKRNAASRLGAGAGDAIDVQGHPFFR--HINWEELLARKVEPPFKPLLQSEEDVSQFDSKFTRQTPVDSPDDATLSESANQVFVGFTYVAPSVLESV 420
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Xenopus
Match: l3hypdh (L-3-hydroxyproline dehydratase (trans-) [Source:Xenbase;Acc:XB-GENE-1000732]) HSP 1 Score: 412.535 bits (1059), Expect = 1.162e-136 Identity = 208/380 (54.74%), Postives = 271/380 (71.32%), Query Frame = 2
Query: 149 DELSIQQFIQNGPLKREED-DDIESVEICQSVVNPGV-KISMEDFKVLNVIGRGGYGKVFLVQKSSSGHDENKLYAMKVLKKARIIRNEKDTAHTKSERNILEMIRHPFLVKLHYAFQNQSNLYIVLTYCSGGELFTYLEREGTLMENQACFYVAEISLAIGHLHKTGIIYRDLKPENVLLDKKGHVKLTDFGLSKESV---SLTNTFCGTIEYMAPEILKRGGHGKEVDWWSLGTLLFDMLSGGPPFSQECDKQETITKILHSTIAYPKYFSSEVVRLIQGLLKRNPKERLGC-KNDVEDIKNHEFFKTHDINWSDVYHRRMDPPFKPELKSEMDVSCFDSTFTGQQPIMTPDDCNGAHILPDLFQGFSYVAPNVASSV 1270
D ++ +F+ +G L E + E EI ++ VN G KI E F++L V+G+GGYGKVF V+K H K++AMKVLKKA I+RN KDTAHTK+ERNILE ++HPF+V L YAFQ LY++L Y SGGELF LEREG ME+ ACFY+AEIS+A+GHLH+ GIIYRDLKPEN++L+ +GHVKLTDFGL KES+ ++T+TFCGTIEYMAPEIL R GH + VDWWSLG L++DML+G PPF+ E ++++TI KIL + P Y + E L++ LLKRN RLG D D++ H FF+ INW ++ R+++PPFKP L+SE DVS FDS FT Q P+ +PDD + +F GF+YVAP+V SV
Sbjct: 47 DGGTLTEFMDHGSLSYELGMEHCEKFEISETSVNRGPEKIRPECFELLRVLGKGGYGKVFQVRKVIGAHT-GKIFAMKVLKKAMIVRNAKDTAHTKAERNILEEVKHPFIVDLIYAFQTGGKLYLILEYLSGGELFMQLEREGIFMEDTACFYLAEISMALGHLHQKGIIYRDLKPENIMLNHQGHVKLTDFGLCKESIHDGTVTHTFCGTIEYMAPEILMRSGHNRAVDWWSLGALMYDMLTGAPPFTGE-NRKKTIDKILKCKLNLPPYLTQEARDLLKKLLKRNAASRLGAGAGDAIDVQGHPFFR--HINWEELLARKVEPPFKPLLQSEEDVSQFDSKFTRQTPVDSPDDATLSESANQVFVGFTYVAPSVLESV 422
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Xenopus
Match: l3hypdh (L-3-hydroxyproline dehydratase (trans-) [Source:Xenbase;Acc:XB-GENE-1000732]) HSP 1 Score: 410.223 bits (1053), Expect = 1.636e-136 Identity = 203/357 (56.86%), Postives = 260/357 (72.83%), Query Frame = 2
Query: 215 ESVEICQSVVNPGV-KISMEDFKVLNVIGRGGYGKVFLVQKSSSGHDENKLYAMKVLKKARIIRNEKDTAHTKSERNILEMIRHPFLVKLHYAFQNQSNLYIVLTYCSGGELFTYLEREGTLMENQACFYVAEISLAIGHLHKTGIIYRDLKPENVLLDKKGHVKLTDFGLSKESV---SLTNTFCGTIEYMAPEILKRGGHGKEVDWWSLGTLLFDMLSGGPPFSQECDKQETITKILHSTIAYPKYFSSEVVRLIQGLLKRNPKERLGC-KNDVEDIKNHEFFKTHDINWSDVYHRRMDPPFKPELKSEMDVSCFDSTFTGQQPIMTPDDCNGAHILPDLFQGFSYVAPNVASSV 1270
E EI ++ VN G KI E F++L V+G+GGYGKVF V+K H K++AMKVLKKA I+RN KDTAHTK+ERNILE ++HPF+V L YAFQ LY++L Y SGGELF LEREG ME+ ACFY+AEIS+A+GHLH+ GIIYRDLKPEN++L+ +GHVKLTDFGL KES+ ++T+TFCGTIEYMAPEIL R GH + VDWWSLG L++DML+G PPF+ E ++++TI KIL + P Y + E L++ LLKRN RLG D D++ H FF+ INW ++ R+++PPFKP L+SE DVS FDS FT Q P+ +PDD + +F GF+YVAP+V SV
Sbjct: 23 EKFEISETSVNRGPEKIRPECFELLRVLGKGGYGKVFQVRKVIGAHT-GKIFAMKVLKKAMIVRNAKDTAHTKAERNILEEVKHPFIVDLIYAFQTGGKLYLILEYLSGGELFMQLEREGIFMEDTACFYLAEISMALGHLHQKGIIYRDLKPENIMLNHQGHVKLTDFGLCKESIHDGTVTHTFCGTIEYMAPEILMRSGHNRAVDWWSLGALMYDMLTGAPPFTGE-NRKKTIDKILKCKLNLPPYLTQEARDLLKKLLKRNAASRLGAGAGDAIDVQGHPFFR--HINWEELLARKVEPPFKPLLQSEEDVSQFDSKFTRQTPVDSPDDATLSESANQVFVGFTYVAPSVLESV 375
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Xenopus
Match: l3hypdh (L-3-hydroxyproline dehydratase (trans-) [Source:Xenbase;Acc:XB-GENE-1000732]) HSP 1 Score: 409.453 bits (1051), Expect = 2.710e-136 Identity = 203/357 (56.86%), Postives = 260/357 (72.83%), Query Frame = 2
Query: 215 ESVEICQSVVNPGV-KISMEDFKVLNVIGRGGYGKVFLVQKSSSGHDENKLYAMKVLKKARIIRNEKDTAHTKSERNILEMIRHPFLVKLHYAFQNQSNLYIVLTYCSGGELFTYLEREGTLMENQACFYVAEISLAIGHLHKTGIIYRDLKPENVLLDKKGHVKLTDFGLSKESV---SLTNTFCGTIEYMAPEILKRGGHGKEVDWWSLGTLLFDMLSGGPPFSQECDKQETITKILHSTIAYPKYFSSEVVRLIQGLLKRNPKERLGC-KNDVEDIKNHEFFKTHDINWSDVYHRRMDPPFKPELKSEMDVSCFDSTFTGQQPIMTPDDCNGAHILPDLFQGFSYVAPNVASSV 1270
E EI ++ VN G KI E F++L V+G+GGYGKVF V+K H K++AMKVLKKA I+RN KDTAHTK+ERNILE ++HPF+V L YAFQ LY++L Y SGGELF LEREG ME+ ACFY+AEIS+A+GHLH+ GIIYRDLKPEN++L+ +GHVKLTDFGL KES+ ++T+TFCGTIEYMAPEIL R GH + VDWWSLG L++DML+G PPF+ E ++++TI KIL + P Y + E L++ LLKRN RLG D D++ H FF+ INW ++ R+++PPFKP L+SE DVS FDS FT Q P+ +PDD + +F GF+YVAP+V SV
Sbjct: 15 EKFEISETSVNRGPEKIRPECFELLRVLGKGGYGKVFQVRKVIGAHT-GKIFAMKVLKKAMIVRNAKDTAHTKAERNILEEVKHPFIVDLIYAFQTGGKLYLILEYLSGGELFMQLEREGIFMEDTACFYLAEISMALGHLHQKGIIYRDLKPENIMLNHQGHVKLTDFGLCKESIHDGTVTHTFCGTIEYMAPEILMRSGHNRAVDWWSLGALMYDMLTGAPPFTGE-NRKKTIDKILKCKLNLPPYLTQEARDLLKKLLKRNAASRLGAGAGDAIDVQGHPFFR--HINWEELLARKVEPPFKPLLQSEEDVSQFDSKFTRQTPVDSPDDATLSESANQVFVGFTYVAPSVLESV 367
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Xenopus
Match: l3hypdh (L-3-hydroxyproline dehydratase (trans-) [Source:Xenbase;Acc:XB-GENE-1000732]) HSP 1 Score: 409.068 bits (1050), Expect = 8.719e-136 Identity = 203/357 (56.86%), Postives = 260/357 (72.83%), Query Frame = 2
Query: 215 ESVEICQSVVNPGV-KISMEDFKVLNVIGRGGYGKVFLVQKSSSGHDENKLYAMKVLKKARIIRNEKDTAHTKSERNILEMIRHPFLVKLHYAFQNQSNLYIVLTYCSGGELFTYLEREGTLMENQACFYVAEISLAIGHLHKTGIIYRDLKPENVLLDKKGHVKLTDFGLSKESV---SLTNTFCGTIEYMAPEILKRGGHGKEVDWWSLGTLLFDMLSGGPPFSQECDKQETITKILHSTIAYPKYFSSEVVRLIQGLLKRNPKERLGC-KNDVEDIKNHEFFKTHDINWSDVYHRRMDPPFKPELKSEMDVSCFDSTFTGQQPIMTPDDCNGAHILPDLFQGFSYVAPNVASSV 1270
E EI ++ VN G KI E F++L V+G+GGYGKVF V+K H K++AMKVLKKA I+RN KDTAHTK+ERNILE ++HPF+V L YAFQ LY++L Y SGGELF LEREG ME+ ACFY+AEIS+A+GHLH+ GIIYRDLKPEN++L+ +GHVKLTDFGL KES+ ++T+TFCGTIEYMAPEIL R GH + VDWWSLG L++DML+G PPF+ E ++++TI KIL + P Y + E L++ LLKRN RLG D D++ H FF+ INW ++ R+++PPFKP L+SE DVS FDS FT Q P+ +PDD + +F GF+YVAP+V SV
Sbjct: 37 EKFEISETSVNRGPEKIRPECFELLRVLGKGGYGKVFQVRKVIGAHT-GKIFAMKVLKKAMIVRNAKDTAHTKAERNILEEVKHPFIVDLIYAFQTGGKLYLILEYLSGGELFMQLEREGIFMEDTACFYLAEISMALGHLHQKGIIYRDLKPENIMLNHQGHVKLTDFGLCKESIHDGTVTHTFCGTIEYMAPEILMRSGHNRAVDWWSLGALMYDMLTGAPPFTGE-NRKKTIDKILKCKLNLPPYLTQEARDLLKKLLKRNAASRLGAGAGDAIDVQGHPFFR--HINWEELLARKVEPPFKPLLQSEEDVSQFDSKFTRQTPVDSPDDATLSESANQVFVGFTYVAPSVLESV 389
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Mouse
Match: Rps6kb1 (ribosomal protein S6 kinase, polypeptide 1 [Source:MGI Symbol;Acc:MGI:1270849]) HSP 1 Score: 406.371 bits (1043), Expect = 1.864e-134 Identity = 202/357 (56.58%), Postives = 262/357 (73.39%), Query Frame = 2
Query: 215 ESVEICQSVVNPGV-KISMEDFKVLNVIGRGGYGKVFLVQKSSSGHDENKLYAMKVLKKARIIRNEKDTAHTKSERNILEMIRHPFLVKLHYAFQNQSNLYIVLTYCSGGELFTYLEREGTLMENQACFYVAEISLAIGHLHKTGIIYRDLKPENVLLDKKGHVKLTDFGLSKESV---SLTNTFCGTIEYMAPEILKRGGHGKEVDWWSLGTLLFDMLSGGPPFSQECDKQETITKILHSTIAYPKYFSSEVVRLIQGLLKRNPKERLGC-KNDVEDIKNHEFFKTHDINWSDVYHRRMDPPFKPELKSEMDVSCFDSTFTGQQPIMTPDDCNGAHILPDLFQGFSYVAPNVASSV 1270
E EI ++ VN G KI E F++L V+G+GGYGKVF V+K + G + K++AMKVLKKA I+RN KDTAHTK+ERNILE ++HPF+V L YAFQ LY++L Y SGGELF LEREG ME+ ACFY+AEIS+A+GHLH+ GIIYRDLKPEN++L+ +GHVKLTDFGL KES+ ++T+TFCGTIEYMAPEIL R GH + VDWWSLG L++DML+G PPF+ E ++++TI KIL + P Y + E L++ LLKRN RLG D +++ H FF+ INW ++ R+++PPFKP L+SE DVS FDS FT Q P+ +PDD + +F GF+YVAP+V SV
Sbjct: 70 EKFEISETSVNRGPEKIRPECFELLRVLGKGGYGKVFQVRKVT-GANTGKIFAMKVLKKAMIVRNAKDTAHTKAERNILEEVKHPFIVDLIYAFQTGGKLYLILEYLSGGELFMQLEREGIFMEDTACFYLAEISMALGHLHQKGIIYRDLKPENIMLNHQGHVKLTDFGLCKESIHDGTVTHTFCGTIEYMAPEILMRSGHNRAVDWWSLGALMYDMLTGAPPFTGE-NRKKTIDKILKCKLNLPPYLTQEARDLLKKLLKRNAASRLGAGPGDAGEVQAHPFFR--HINWEELLARKVEPPFKPLLQSEEDVSQFDSKFTRQTPVDSPDDSTLSESANQVFLGFTYVAPSVLESV 422
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Mouse
Match: Rps6kb2 (ribosomal protein S6 kinase, polypeptide 2 [Source:MGI Symbol;Acc:MGI:1927343]) HSP 1 Score: 399.823 bits (1026), Expect = 1.846e-132 Identity = 193/357 (54.06%), Postives = 261/357 (73.11%), Query Frame = 2
Query: 215 ESVEICQSVVNPGV-KISMEDFKVLNVIGRGGYGKVFLVQKSSSGHDENKLYAMKVLKKARIIRNEKDTAHTKSERNILEMIRHPFLVKLHYAFQNQSNLYIVLTYCSGGELFTYLEREGTLMENQACFYVAEISLAIGHLHKTGIIYRDLKPENVLLDKKGHVKLTDFGLSKESV---SLTNTFCGTIEYMAPEILKRGGHGKEVDWWSLGTLLFDMLSGGPPFSQECDKQETITKILHSTIAYPKYFSSEVVRLIQGLLKRNPKERLGCK-NDVEDIKNHEFFKTHDINWSDVYHRRMDPPFKPELKSEMDVSCFDSTFTGQQPIMTPDDCNGAHILPDLFQGFSYVAPNVASSV 1270
E VE+ +S VN G +I F++L+V+G+GGYGKVF V+K G + K+YAMKVL+KA+I+ + KDTAHT++ERNILE ++HPF+V+L YAFQ LY++L SGGELFT+LEREG +E+ ACFY+AEI+LA+GHLH GIIYRDLKPEN++L +GH+KLTDFGL KES+ ++T+TFCGTIEYMAPEIL R GH + VDWWSLG L++DML+G PPF+ E ++++T+ KI+ + P Y + + L + LKRNP +R+G D D++ H FF+ INW D+ RR+DPPF+P L+SE DVS FD+ FT Q P+ +PDD + F GF+YVAP+V S+
Sbjct: 46 EEVELTESSVNLGPERIGPHCFELLSVLGKGGYGKVFQVRKVQ-GTNLGKIYAMKVLRKAKIVCSAKDTAHTRAERNILESVKHPFIVELAYAFQTGGKLYLILECLSGGELFTHLEREGIFLEDTACFYLAEITLALGHLHSHGIIYRDLKPENIMLSSQGHIKLTDFGLCKESIHEGAITHTFCGTIEYMAPEILVRTGHNRAVDWWSLGALMYDMLTGSPPFTAE-NRKKTMDKIIKGKLVLPPYLTPDARDLAKKFLKRNPTQRIGGGLGDAADVQRHPFFR--HINWDDLLARRVDPPFRPSLQSEEDVSQFDARFTRQTPVDSPDDTALSESANQAFLGFTYVAPSVLDSI 398
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Mouse
Match: Rps6kb2 (ribosomal protein S6 kinase, polypeptide 2 [Source:MGI Symbol;Acc:MGI:1927343]) HSP 1 Score: 399.823 bits (1026), Expect = 1.846e-132 Identity = 193/357 (54.06%), Postives = 261/357 (73.11%), Query Frame = 2
Query: 215 ESVEICQSVVNPGV-KISMEDFKVLNVIGRGGYGKVFLVQKSSSGHDENKLYAMKVLKKARIIRNEKDTAHTKSERNILEMIRHPFLVKLHYAFQNQSNLYIVLTYCSGGELFTYLEREGTLMENQACFYVAEISLAIGHLHKTGIIYRDLKPENVLLDKKGHVKLTDFGLSKESV---SLTNTFCGTIEYMAPEILKRGGHGKEVDWWSLGTLLFDMLSGGPPFSQECDKQETITKILHSTIAYPKYFSSEVVRLIQGLLKRNPKERLGCK-NDVEDIKNHEFFKTHDINWSDVYHRRMDPPFKPELKSEMDVSCFDSTFTGQQPIMTPDDCNGAHILPDLFQGFSYVAPNVASSV 1270
E VE+ +S VN G +I F++L+V+G+GGYGKVF V+K G + K+YAMKVL+KA+I+ + KDTAHT++ERNILE ++HPF+V+L YAFQ LY++L SGGELFT+LEREG +E+ ACFY+AEI+LA+GHLH GIIYRDLKPEN++L +GH+KLTDFGL KES+ ++T+TFCGTIEYMAPEIL R GH + VDWWSLG L++DML+G PPF+ E ++++T+ KI+ + P Y + + L + LKRNP +R+G D D++ H FF+ INW D+ RR+DPPF+P L+SE DVS FD+ FT Q P+ +PDD + F GF+YVAP+V S+
Sbjct: 46 EEVELTESSVNLGPERIGPHCFELLSVLGKGGYGKVFQVRKVQ-GTNLGKIYAMKVLRKAKIVCSAKDTAHTRAERNILESVKHPFIVELAYAFQTGGKLYLILECLSGGELFTHLEREGIFLEDTACFYLAEITLALGHLHSHGIIYRDLKPENIMLSSQGHIKLTDFGLCKESIHEGAITHTFCGTIEYMAPEILVRTGHNRAVDWWSLGALMYDMLTGSPPFTAE-NRKKTMDKIIKGKLVLPPYLTPDARDLAKKFLKRNPTQRIGGGLGDAADVQRHPFFR--HINWDDLLARRVDPPFRPSLQSEEDVSQFDARFTRQTPVDSPDDTALSESANQAFLGFTYVAPSVLDSI 398
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Mouse
Match: Rps6kb2 (ribosomal protein S6 kinase, polypeptide 2 [Source:MGI Symbol;Acc:MGI:1927343]) HSP 1 Score: 383.645 bits (984), Expect = 1.767e-127 Identity = 184/332 (55.42%), Postives = 248/332 (74.70%), Query Frame = 2
Query: 215 ESVEICQSVVNPGV-KISMEDFKVLNVIGRGGYGKVFLVQKSSSGHDENKLYAMKVLKKARIIRNEKDTAHTKSERNILEMIRHPFLVKLHYAFQNQSNLYIVLTYCSGGELFTYLEREGTLMENQACFYVAEISLAIGHLHKTGIIYRDLKPENVLLDKKGHVKLTDFGLSKESV---SLTNTFCGTIEYMAPEILKRGGHGKEVDWWSLGTLLFDMLSGGPPFSQECDKQETITKILHSTIAYPKYFSSEVVRLIQGLLKRNPKERLGCK-NDVEDIKNHEFFKTHDINWSDVYHRRMDPPFKPELKSEMDVSCFDSTFTGQQPIMTPDD 1195
E VE+ +S VN G +I F++L+V+G+GGYGKVF V+K G + K+YAMKVL+KA+I+ + KDTAHT++ERNILE ++HPF+V+L YAFQ LY++L SGGELFT+LEREG +E+ ACFY+AEI+LA+GHLH GIIYRDLKPEN++L +GH+KLTDFGL KES+ ++T+TFCGTIEYMAPEIL R GH + VDWWSLG L++DML+G PPF+ E ++++T+ KI+ + P Y + + L + LKRNP +R+G D D++ H FF+ INW D+ RR+DPPF+P L+SE DVS FD+ FT Q P+ +PDD
Sbjct: 46 EEVELTESSVNLGPERIGPHCFELLSVLGKGGYGKVFQVRKVQ-GTNLGKIYAMKVLRKAKIVCSAKDTAHTRAERNILESVKHPFIVELAYAFQTGGKLYLILECLSGGELFTHLEREGIFLEDTACFYLAEITLALGHLHSHGIIYRDLKPENIMLSSQGHIKLTDFGLCKESIHEGAITHTFCGTIEYMAPEILVRTGHNRAVDWWSLGALMYDMLTGSPPFTAE-NRKKTMDKIIKGKLVLPPYLTPDARDLAKKFLKRNPTQRIGGGLGDAADVQRHPFFR--HINWDDLLARRVDPPFRPSLQSEEDVSQFDARFTRQTPVDSPDD 373
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Mouse
Match: Rps6kb2 (ribosomal protein S6 kinase, polypeptide 2 [Source:MGI Symbol;Acc:MGI:1927343]) HSP 1 Score: 383.645 bits (984), Expect = 1.767e-127 Identity = 184/332 (55.42%), Postives = 248/332 (74.70%), Query Frame = 2
Query: 215 ESVEICQSVVNPGV-KISMEDFKVLNVIGRGGYGKVFLVQKSSSGHDENKLYAMKVLKKARIIRNEKDTAHTKSERNILEMIRHPFLVKLHYAFQNQSNLYIVLTYCSGGELFTYLEREGTLMENQACFYVAEISLAIGHLHKTGIIYRDLKPENVLLDKKGHVKLTDFGLSKESV---SLTNTFCGTIEYMAPEILKRGGHGKEVDWWSLGTLLFDMLSGGPPFSQECDKQETITKILHSTIAYPKYFSSEVVRLIQGLLKRNPKERLGCK-NDVEDIKNHEFFKTHDINWSDVYHRRMDPPFKPELKSEMDVSCFDSTFTGQQPIMTPDD 1195
E VE+ +S VN G +I F++L+V+G+GGYGKVF V+K G + K+YAMKVL+KA+I+ + KDTAHT++ERNILE ++HPF+V+L YAFQ LY++L SGGELFT+LEREG +E+ ACFY+AEI+LA+GHLH GIIYRDLKPEN++L +GH+KLTDFGL KES+ ++T+TFCGTIEYMAPEIL R GH + VDWWSLG L++DML+G PPF+ E ++++T+ KI+ + P Y + + L + LKRNP +R+G D D++ H FF+ INW D+ RR+DPPF+P L+SE DVS FD+ FT Q P+ +PDD
Sbjct: 46 EEVELTESSVNLGPERIGPHCFELLSVLGKGGYGKVFQVRKVQ-GTNLGKIYAMKVLRKAKIVCSAKDTAHTRAERNILESVKHPFIVELAYAFQTGGKLYLILECLSGGELFTHLEREGIFLEDTACFYLAEITLALGHLHSHGIIYRDLKPENIMLSSQGHIKLTDFGLCKESIHEGAITHTFCGTIEYMAPEILVRTGHNRAVDWWSLGALMYDMLTGSPPFTAE-NRKKTMDKIIKGKLVLPPYLTPDARDLAKKFLKRNPTQRIGGGLGDAADVQRHPFFR--HINWDDLLARRVDPPFRPSLQSEEDVSQFDARFTRQTPVDSPDD 373
BLAST of Non-specific serine/threonine protein kinase vs. UniProt/SwissProt
Match: sp|P23443|KS6B1_HUMAN (Ribosomal protein S6 kinase beta-1 OS=Homo sapiens OX=9606 GN=RPS6KB1 PE=1 SV=2) HSP 1 Score: 407.142 bits (1045), Expect = 8.030e-134 Identity = 202/357 (56.58%), Postives = 262/357 (73.39%), Query Frame = 2
Query: 215 ESVEICQSVVNPGV-KISMEDFKVLNVIGRGGYGKVFLVQKSSSGHDENKLYAMKVLKKARIIRNEKDTAHTKSERNILEMIRHPFLVKLHYAFQNQSNLYIVLTYCSGGELFTYLEREGTLMENQACFYVAEISLAIGHLHKTGIIYRDLKPENVLLDKKGHVKLTDFGLSKESV---SLTNTFCGTIEYMAPEILKRGGHGKEVDWWSLGTLLFDMLSGGPPFSQECDKQETITKILHSTIAYPKYFSSEVVRLIQGLLKRNPKERLGC-KNDVEDIKNHEFFKTHDINWSDVYHRRMDPPFKPELKSEMDVSCFDSTFTGQQPIMTPDDCNGAHILPDLFQGFSYVAPNVASSV 1270
E EI ++ VN G KI E F++L V+G+GGYGKVF V+K + G + K++AMKVLKKA I+RN KDTAHTK+ERNILE ++HPF+V L YAFQ LY++L Y SGGELF LEREG ME+ ACFY+AEIS+A+GHLH+ GIIYRDLKPEN++L+ +GHVKLTDFGL KES+ ++T+TFCGTIEYMAPEIL R GH + VDWWSLG L++DML+G PPF+ E ++++TI KIL + P Y + E L++ LLKRN RLG D +++ H FF+ INW ++ R+++PPFKP L+SE DVS FDS FT Q P+ +PDD + +F GF+YVAP+V SV
Sbjct: 70 EKFEISETSVNRGPEKIRPECFELLRVLGKGGYGKVFQVRKVT-GANTGKIFAMKVLKKAMIVRNAKDTAHTKAERNILEEVKHPFIVDLIYAFQTGGKLYLILEYLSGGELFMQLEREGIFMEDTACFYLAEISMALGHLHQKGIIYRDLKPENIMLNHQGHVKLTDFGLCKESIHDGTVTHTFCGTIEYMAPEILMRSGHNRAVDWWSLGALMYDMLTGAPPFTGE-NRKKTIDKILKCKLNLPPYLTQEARDLLKKLLKRNAASRLGAGPGDAGEVQAHPFFR--HINWEELLARKVEPPFKPLLQSEEDVSQFDSKFTRQTPVDSPDDSTLSESANQVFLGFTYVAPSVLESV 422
BLAST of Non-specific serine/threonine protein kinase vs. UniProt/SwissProt
Match: sp|P67998|KS6B1_RABIT (Ribosomal protein S6 kinase beta-1 OS=Oryctolagus cuniculus OX=9986 GN=RPS6KB1 PE=2 SV=1) HSP 1 Score: 406.757 bits (1044), Expect = 9.237e-134 Identity = 202/357 (56.58%), Postives = 262/357 (73.39%), Query Frame = 2
Query: 215 ESVEICQSVVNPGV-KISMEDFKVLNVIGRGGYGKVFLVQKSSSGHDENKLYAMKVLKKARIIRNEKDTAHTKSERNILEMIRHPFLVKLHYAFQNQSNLYIVLTYCSGGELFTYLEREGTLMENQACFYVAEISLAIGHLHKTGIIYRDLKPENVLLDKKGHVKLTDFGLSKESV---SLTNTFCGTIEYMAPEILKRGGHGKEVDWWSLGTLLFDMLSGGPPFSQECDKQETITKILHSTIAYPKYFSSEVVRLIQGLLKRNPKERLGC-KNDVEDIKNHEFFKTHDINWSDVYHRRMDPPFKPELKSEMDVSCFDSTFTGQQPIMTPDDCNGAHILPDLFQGFSYVAPNVASSV 1270
E EI ++ VN G KI E F++L V+G+GGYGKVF V+K + G + K++AMKVLKKA I+RN KDTAHTK+ERNILE ++HPF+V L YAFQ LY++L Y SGGELF LEREG ME+ ACFY+AEIS+A+GHLH+ GIIYRDLKPEN++L+ +GHVKLTDFGL KES+ ++T+TFCGTIEYMAPEIL R GH + VDWWSLG L++DML+G PPF+ E ++++TI KIL + P Y + E L++ LLKRN RLG D +++ H FF+ INW ++ R+++PPFKP L+SE DVS FDS FT Q P+ +PDD + +F GF+YVAP+V SV
Sbjct: 70 EKFEISETSVNRGPEKIRPECFELLRVLGKGGYGKVFQVRKVT-GANTGKIFAMKVLKKAMIVRNAKDTAHTKAERNILEEVKHPFIVDLIYAFQTGGKLYLILEYLSGGELFMQLEREGIFMEDTACFYLAEISMALGHLHQKGIIYRDLKPENIMLNHQGHVKLTDFGLCKESIHDGTVTHTFCGTIEYMAPEILMRSGHNRAVDWWSLGALMYDMLTGAPPFTGE-NRKKTIDKILKCKLNLPPYLTQEARDLLKKLLKRNAASRLGAGPGDAGEVQAHPFFR--HINWEELLARKVEPPFKPLLQSEEDVSQFDSKFTRQTPVDSPDDSTLSESANQVFLGFTYVAPSVLESV 422
BLAST of Non-specific serine/threonine protein kinase vs. UniProt/SwissProt
Match: sp|P67999|KS6B1_RAT (Ribosomal protein S6 kinase beta-1 OS=Rattus norvegicus OX=10116 GN=Rps6kb1 PE=1 SV=1) HSP 1 Score: 406.757 bits (1044), Expect = 1.007e-133 Identity = 202/357 (56.58%), Postives = 262/357 (73.39%), Query Frame = 2
Query: 215 ESVEICQSVVNPGV-KISMEDFKVLNVIGRGGYGKVFLVQKSSSGHDENKLYAMKVLKKARIIRNEKDTAHTKSERNILEMIRHPFLVKLHYAFQNQSNLYIVLTYCSGGELFTYLEREGTLMENQACFYVAEISLAIGHLHKTGIIYRDLKPENVLLDKKGHVKLTDFGLSKESV---SLTNTFCGTIEYMAPEILKRGGHGKEVDWWSLGTLLFDMLSGGPPFSQECDKQETITKILHSTIAYPKYFSSEVVRLIQGLLKRNPKERLGC-KNDVEDIKNHEFFKTHDINWSDVYHRRMDPPFKPELKSEMDVSCFDSTFTGQQPIMTPDDCNGAHILPDLFQGFSYVAPNVASSV 1270
E EI ++ VN G KI E F++L V+G+GGYGKVF V+K + G + K++AMKVLKKA I+RN KDTAHTK+ERNILE ++HPF+V L YAFQ LY++L Y SGGELF LEREG ME+ ACFY+AEIS+A+GHLH+ GIIYRDLKPEN++L+ +GHVKLTDFGL KES+ ++T+TFCGTIEYMAPEIL R GH + VDWWSLG L++DML+G PPF+ E ++++TI KIL + P Y + E L++ LLKRN RLG D +++ H FF+ INW ++ R+++PPFKP L+SE DVS FDS FT Q P+ +PDD + +F GF+YVAP+V SV
Sbjct: 70 EKFEISETSVNRGPEKIRPECFELLRVLGKGGYGKVFQVRKVT-GANTGKIFAMKVLKKAMIVRNAKDTAHTKAERNILEEVKHPFIVDLIYAFQTGGKLYLILEYLSGGELFMQLEREGIFMEDTACFYLAEISMALGHLHQKGIIYRDLKPENIMLNHQGHVKLTDFGLCKESIHDGTVTHTFCGTIEYMAPEILMRSGHNRAVDWWSLGALMYDMLTGAPPFTGE-NRKKTIDKILKCKLNLPPYLTQEARDLLKKLLKRNAASRLGAGPGDAGEVQAHPFFR--HINWEELLARKVEPPFKPLLQSEEDVSQFDSKFTRQTPVDSPDDSTLSESANQVFLGFTYVAPSVLESV 422
BLAST of Non-specific serine/threonine protein kinase vs. UniProt/SwissProt
Match: sp|Q8BSK8|KS6B1_MOUSE (Ribosomal protein S6 kinase beta-1 OS=Mus musculus OX=10090 GN=Rps6kb1 PE=1 SV=2) HSP 1 Score: 406.371 bits (1043), Expect = 1.304e-133 Identity = 202/357 (56.58%), Postives = 262/357 (73.39%), Query Frame = 2
Query: 215 ESVEICQSVVNPGV-KISMEDFKVLNVIGRGGYGKVFLVQKSSSGHDENKLYAMKVLKKARIIRNEKDTAHTKSERNILEMIRHPFLVKLHYAFQNQSNLYIVLTYCSGGELFTYLEREGTLMENQACFYVAEISLAIGHLHKTGIIYRDLKPENVLLDKKGHVKLTDFGLSKESV---SLTNTFCGTIEYMAPEILKRGGHGKEVDWWSLGTLLFDMLSGGPPFSQECDKQETITKILHSTIAYPKYFSSEVVRLIQGLLKRNPKERLGC-KNDVEDIKNHEFFKTHDINWSDVYHRRMDPPFKPELKSEMDVSCFDSTFTGQQPIMTPDDCNGAHILPDLFQGFSYVAPNVASSV 1270
E EI ++ VN G KI E F++L V+G+GGYGKVF V+K + G + K++AMKVLKKA I+RN KDTAHTK+ERNILE ++HPF+V L YAFQ LY++L Y SGGELF LEREG ME+ ACFY+AEIS+A+GHLH+ GIIYRDLKPEN++L+ +GHVKLTDFGL KES+ ++T+TFCGTIEYMAPEIL R GH + VDWWSLG L++DML+G PPF+ E ++++TI KIL + P Y + E L++ LLKRN RLG D +++ H FF+ INW ++ R+++PPFKP L+SE DVS FDS FT Q P+ +PDD + +F GF+YVAP+V SV
Sbjct: 70 EKFEISETSVNRGPEKIRPECFELLRVLGKGGYGKVFQVRKVT-GANTGKIFAMKVLKKAMIVRNAKDTAHTKAERNILEEVKHPFIVDLIYAFQTGGKLYLILEYLSGGELFMQLEREGIFMEDTACFYLAEISMALGHLHQKGIIYRDLKPENIMLNHQGHVKLTDFGLCKESIHDGTVTHTFCGTIEYMAPEILMRSGHNRAVDWWSLGALMYDMLTGAPPFTGE-NRKKTIDKILKCKLNLPPYLTQEARDLLKKLLKRNAASRLGAGPGDAGEVQAHPFFR--HINWEELLARKVEPPFKPLLQSEEDVSQFDSKFTRQTPVDSPDDSTLSESANQVFLGFTYVAPSVLESV 422
BLAST of Non-specific serine/threonine protein kinase vs. UniProt/SwissProt
Match: sp|Q6TJY3|KS6B1_BOVIN (Ribosomal protein S6 kinase beta-1 OS=Bos taurus OX=9913 GN=RPS6KB1 PE=2 SV=1) HSP 1 Score: 406.371 bits (1043), Expect = 1.330e-133 Identity = 202/357 (56.58%), Postives = 262/357 (73.39%), Query Frame = 2
Query: 215 ESVEICQSVVNPGV-KISMEDFKVLNVIGRGGYGKVFLVQKSSSGHDENKLYAMKVLKKARIIRNEKDTAHTKSERNILEMIRHPFLVKLHYAFQNQSNLYIVLTYCSGGELFTYLEREGTLMENQACFYVAEISLAIGHLHKTGIIYRDLKPENVLLDKKGHVKLTDFGLSKESV---SLTNTFCGTIEYMAPEILKRGGHGKEVDWWSLGTLLFDMLSGGPPFSQECDKQETITKILHSTIAYPKYFSSEVVRLIQGLLKRNPKERLGC-KNDVEDIKNHEFFKTHDINWSDVYHRRMDPPFKPELKSEMDVSCFDSTFTGQQPIMTPDDCNGAHILPDLFQGFSYVAPNVASSV 1270
E EI ++ VN G KI E F++L V+G+GGYGKVF V+K + G + K++AMKVLKKA I+RN KDTAHTK+ERNILE ++HPF+V L YAFQ LY++L Y SGGELF LEREG ME+ ACFY+AEIS+A+GHLH+ GIIYRDLKPEN++L+ +GHVKLTDFGL KES+ ++T+TFCGTIEYMAPEIL R GH + VDWWSLG L++DML+G PPF+ E ++++TI KIL + P Y + E L++ LLKRN RLG D +++ H FF+ INW ++ R+++PPFKP L+SE DVS FDS FT Q P+ +PDD + +F GF+YVAP+V SV
Sbjct: 70 EKFEISETSVNRGPEKIRPECFELLRVLGKGGYGKVFQVRKVT-GANTGKIFAMKVLKKAMIVRNAKDTAHTKAERNILEEVKHPFIVDLIYAFQTGGKLYLILEYLSGGELFMQLEREGIFMEDTACFYLAEISMALGHLHQKGIIYRDLKPENIMLNHQGHVKLTDFGLCKESIHDGTVTHTFCGTIEYMAPEILMRSGHNRAVDWWSLGALMYDMLTGAPPFTGE-NRKKTIDKILKCKLNLPPYLTQEARDLLKKLLKRNAASRLGAGPGDAGEVQAHPFFR--HINWEELLARKVEPPFKPLLQSEEDVSQFDSKFTRQTPVDSPDDSALSESANQVFLGFTYVAPSVLESV 422
BLAST of Non-specific serine/threonine protein kinase vs. TrEMBL
Match: A0A4S2MBY0 (Non-specific serine/threonine protein kinase OS=Opisthorchis felineus OX=147828 GN=CRM22_001136 PE=4 SV=1) HSP 1 Score: 547.354 bits (1409), Expect = 0.000e+0 Identity = 273/454 (60.13%), Postives = 326/454 (71.81%), Query Frame = 2
Query: 104 DIVSKDNDFIRLSERDELSIQQFIQNGPLKREEDDDIESVEICQSVVNPGVKISMEDFKVLNVIGRGGYGKVFLVQKSSSGHDENKLYAMKVLKKARIIRNEKDTAHTKSERNILEMIRHPFLVKLHYAFQNQSNLYIVLTYCSGGELFTYLEREGTLMENQACFYVAEISLAIGHLHKTGIIYRDLKPENVLLDKKGHVKLTDFGLSKESVSLTNTFCGTIEYMAPEILKRGGHGKEVDWWSLGTLLFDMLSGGPPFSQECDKQETITKILHSTIAYPKYFSSEVVRLIQGLLKRNPKERLGCKNDVEDIKNHEFFKTHDINWSDVYHRRMDPPFKPELKSEMDVSCFDSTFTGQQPIMTPDDCNGAHILPDLFQGFSYVAPNVASSVLNEPMTGVSRHTRR---RNNSGLSGNAFNGLNANSPFLNENDFIEMSCNDAYVNQPVTNALTYFP 1456
D S+ +D S+R E + QQ +QN P EE+ E+VEICQ VNP ++ E+F + VIG+GGYGKVFLVQK G D+ KLYAMKVLKKAR++ NEKD AHT SERNIL+M+RHPFLV+LHYAFQN SNLYIVL YC GGELF YLEREG L+EN ACFY AEI LAIGHLH GIIYRDLK ENVLLD++GHVKLTDFGLSKE V TNTFCGTIEYMAPEI++ GHGK VDWWSLGTLL+DMLSG PPFSQE K+ T KIL + + +P FS EVV LI+GLL+R+PKERLG K DVE++K H+FF+ H+INWSDV+HRR+ PPF+P+L ++ DVS FD TFT QP+++P + N I PD+FQGFSYV P+V V+ EP TRR RN + LS + NE + D QPV + LT P
Sbjct: 19 DEESECSDAKSGSQRTERNCQQKLQNEPFS-EENGRKETVEICQQTVNPNNRVGSENFMIQRVIGKGGYGKVFLVQKVD-GKDKGKLYAMKVLKKARLVCNEKDKAHTVSERNILQMLRHPFLVRLHYAFQNHSNLYIVLEYCPGGELFNYLEREGALLENAACFYTAEIILAIGHLHSLGIIYRDLKSENVLLDRQGHVKLTDFGLSKEGVGTTNTFCGTIEYMAPEIIRCEGHGKAVDWWSLGTLLYDMLSGAPPFSQEESKEATTHKILTAPLKFPPAFSPEVVSLIRGLLRRDPKERLGSKEDVEEVKRHKFFRRHEINWSDVFHRRLRPPFRPKLAADNDVSMFDPTFTRLQPVVSPAE-NSRPIPPDMFQGFSYVGPSVCEEVVREPWVDADAGTRRRQYRNQAALSAGRLRRMELAPSHWNEKP--QNRLGDVRPAQPVHSELTSLP 467
BLAST of Non-specific serine/threonine protein kinase vs. TrEMBL
Match: A0A419PD32 (Non-specific serine/threonine protein kinase OS=Clonorchis sinensis OX=79923 GN=Rps6kb2 PE=4 SV=1) HSP 1 Score: 531.561 bits (1368), Expect = 1.918e-177 Identity = 261/418 (62.44%), Postives = 308/418 (73.68%), Query Frame = 2
Query: 215 ESVEICQSVVNPGVKISMEDFKVLNVIGRGGYGKVFLVQKSSSGHDENKLYAMKVLKKARIIRNEKDTAHTKSERNILEMIR-HPFLVKLHYAFQNQSNLYIVLTYCSGGELFTYLEREGTLMENQACFYVAEISLAIGHLHKTGIIYRDLKPENVLLDKKGHVKLTDFGLSKESVSLTNTFCGTIEYMAPEILKRGGHGKEVDWWSLGTLLFDMLSGGPPFSQECDKQETITKILHSTIAYPKYFSSEVVRLIQGLLKRNPKERLGCKNDVEDIKNHEFFKTHDINWSDVYHRRMDPPFKPELKSEMDVSCFDSTFTGQQPIMTPDDCNGAHILPDLFQGFSYVAPNVASSVLNEPMTGVSRHTRR---RNNSGLSGNAFNGLNANSPFLNENDFIEMSCNDAYVNQPVTNALTYFP 1456
E+VEICQ VNP ++ E+F + VIG+GGYGKVFLVQK G D+ KLYAMKVLKKAR++ NEKD AHT SERNIL+M+R HPFLV+LHYAFQN SNLYIVL YC GGELF YLEREG L+EN ACFY AEI LAIGHLH GIIYRDLK ENVLLD++GHVKLTDFGLSKE V TNTFCGTIEYMAPEI++ GHGK VDWWSLGTLL+DMLSG PPFSQE K+ T KIL + + +P FS EVV LI+GLL+R+PKERLG K DVE++K H+FF+ H+INWSDV+HRR+ PPF+P+L ++ DVS FD TFT QP+++P + N I PD+FQGFSYV P+V V+ EP TRR RN + LS + NE + DA QPV + LT P
Sbjct: 33 ETVEICQQTVNPNNRVGSENFMIQRVIGKGGYGKVFLVQKVD-GKDKGKLYAMKVLKKARLVCNEKDKAHTVSERNILQMLRQHPFLVRLHYAFQNHSNLYIVLEYCPGGELFNYLEREGALLENAACFYTAEIILAIGHLHSLGIIYRDLKSENVLLDRQGHVKLTDFGLSKEGVGTTNTFCGTIEYMAPEIIRCEGHGKAVDWWSLGTLLYDMLSGAPPFSQEESKEATTHKILTAPLKFPPAFSPEVVSLIRGLLRRDPKERLGSKEDVEEVKRHKFFRRHEINWSDVFHRRLRPPFRPKLAADNDVSMFDPTFTRLQPVVSPAE-NSRPIPPDMFQGFSYVGPSVCEEVMREPWVDGDAGTRRRQYRNQAALSAGRLRRMELAPSHWNERP--QSRLGDARPAQPVHSELTSLP 446
BLAST of Non-specific serine/threonine protein kinase vs. TrEMBL
Match: C1LJV7 (Non-specific serine/threonine protein kinase OS=Schistosoma japonicum OX=6182 PE=2 SV=1) HSP 1 Score: 518.85 bits (1335), Expect = 6.139e-173 Identity = 257/404 (63.61%), Postives = 307/404 (75.99%), Query Frame = 2
Query: 179 NGPLKREEDDDIESVEICQSVVNPGVKISMEDFKVLNVIGRGGYGKVFLVQKSSSGHDENKLYAMKVLKKARIIRNEKDTAHTKSERNILEMIRHPFLVKLHYAFQNQSNLYIVLTYCSGGELFTYLEREGTLMENQACFYVAEISLAIGHLHKTGIIYRDLKPENVLLDKKGHVKLTDFGLSKESVSLTNTFCGTIEYMAPEILKRGGHGKEVDWWSLGTLLFDMLSGGPPFSQECDKQETITKILHSTIAYPKYFSSEVVRLIQGLLKRNPKERLGCKNDVEDIKNHEFFKTHDINWSDVYHRRMDPPFKPELKSEMDVSCFDSTFTGQQPIMTPDDCNGAHILPDLFQGFSYVAPNVASSVLNEPMTG---VSRHTRRRNNSGLSGN-----AFNGLNANS 1366
N +K+ E + E+VEICQ VNP +++ E+F + VIG+GGYGKVFLVQK G D+ KLYAMKVLKKAR++ +EKD AHT SERNILEM++HPFLVKLHYAFQN SNLYIVL YC GGELF YLEREG+L+EN ACFY +EI LAIGHLH GIIYRDLK ENVLLD++GHVKLTDFGLSKE V TNTFCGTIEYMAPEI++ GHGKEVDWWSLGTLLFDMLSG PPFSQE ++ T KIL + + +P FSSEV+ LI+GLLKR+P ERLG + D+E+IK H+FFK H+INWSDVY+RR+ PPF+P+L +E DVS FD TFT +P+++P D A I PD+FQGFSYVAP+V L + SRH R +S F LNA+S
Sbjct: 46 NSSVKKNEIKNQETVEICQKTVNPNNRVNAENFNIQRVIGKGGYGKVFLVQKVD-GVDQGKLYAMKVLKKARLVCSEKDKAHTVSERNILEMLQHPFLVKLHYAFQNHSNLYIVLEYCHGGELFNYLEREGSLLENAACFYASEIVLAIGHLHSLGIIYRDLKSENVLLDRQGHVKLTDFGLSKEGVCTTNTFCGTIEYMAPEIIRCEGHGKEVDWWSLGTLLFDMLSGAPPFSQEESREATTLKILTAPLKFPPSFSSEVISLIRGLLKRDPNERLGSREDMEEIKRHKFFKRHEINWSDVYNRRLKPPFRPKLTAENDVSMFDPTFTRLRPVVSPVD-GVASIPPDMFQGFSYVAPSVCEDSLRDNTWTDNLPSRHKRHGLRGAMSAGRLRRIGFGHLNASS 447
BLAST of Non-specific serine/threonine protein kinase vs. TrEMBL
Match: C1LJV8 (Non-specific serine/threonine protein kinase OS=Schistosoma japonicum OX=6182 PE=2 SV=1) HSP 1 Score: 519.62 bits (1337), Expect = 9.528e-173 Identity = 257/404 (63.61%), Postives = 307/404 (75.99%), Query Frame = 2
Query: 179 NGPLKREEDDDIESVEICQSVVNPGVKISMEDFKVLNVIGRGGYGKVFLVQKSSSGHDENKLYAMKVLKKARIIRNEKDTAHTKSERNILEMIRHPFLVKLHYAFQNQSNLYIVLTYCSGGELFTYLEREGTLMENQACFYVAEISLAIGHLHKTGIIYRDLKPENVLLDKKGHVKLTDFGLSKESVSLTNTFCGTIEYMAPEILKRGGHGKEVDWWSLGTLLFDMLSGGPPFSQECDKQETITKILHSTIAYPKYFSSEVVRLIQGLLKRNPKERLGCKNDVEDIKNHEFFKTHDINWSDVYHRRMDPPFKPELKSEMDVSCFDSTFTGQQPIMTPDDCNGAHILPDLFQGFSYVAPNVASSVLNEPMTG---VSRHTRRRNNSGLSGN-----AFNGLNANS 1366
N +K+ E + E+VEICQ VNP +++ E+F + VIG+GGYGKVFLVQK G D+ KLYAMKVLKKAR++ +EKD AHT SERNILEM++HPFLVKLHYAFQN SNLYIVL YC GGELF YLEREG+L+EN ACFY +EI LAIGHLH GIIYRDLK ENVLLD++GHVKLTDFGLSKE V TNTFCGTIEYMAPEI++ GHGKEVDWWSLGTLLFDMLSG PPFSQE ++ T KIL + + +P FSSEV+ LI+GLLKR+P ERLG + D+E+IK H+FFK H+INWSDVY+RR+ PPF+P+L +E DVS FD TFT +P+++P D A I PD+FQGFSYVAP+V L + SRH R +S F LNA+S
Sbjct: 46 NSSVKKNEIKNQETVEICQKTVNPNNRVNAENFNIQRVIGKGGYGKVFLVQKVD-GVDQGKLYAMKVLKKARLVCSEKDKAHTVSERNILEMLQHPFLVKLHYAFQNHSNLYIVLEYCHGGELFNYLEREGSLLENAACFYASEIVLAIGHLHSLGIIYRDLKSENVLLDRQGHVKLTDFGLSKEGVCTTNTFCGTIEYMAPEIIRCEGHGKEVDWWSLGTLLFDMLSGAPPFSQEESREATTLKILTAPLKFPPSFSSEVISLIRGLLKRDPNERLGSREDMEEIKRHKFFKRHEINWSDVYNRRLKPPFRPKLTAENDVSMFDPTFTRLRPVVSPVD-GVASIPPDMFQGFSYVAPSVCEDSLRDNTWTDNLPSRHKRHGLRGAMSAGRLRRIGFGHLNASS 447
BLAST of Non-specific serine/threonine protein kinase vs. TrEMBL
Match: C1LJV6 (Non-specific serine/threonine protein kinase OS=Schistosoma japonicum OX=6182 PE=2 SV=1) HSP 1 Score: 519.62 bits (1337), Expect = 9.736e-173 Identity = 257/404 (63.61%), Postives = 307/404 (75.99%), Query Frame = 2
Query: 179 NGPLKREEDDDIESVEICQSVVNPGVKISMEDFKVLNVIGRGGYGKVFLVQKSSSGHDENKLYAMKVLKKARIIRNEKDTAHTKSERNILEMIRHPFLVKLHYAFQNQSNLYIVLTYCSGGELFTYLEREGTLMENQACFYVAEISLAIGHLHKTGIIYRDLKPENVLLDKKGHVKLTDFGLSKESVSLTNTFCGTIEYMAPEILKRGGHGKEVDWWSLGTLLFDMLSGGPPFSQECDKQETITKILHSTIAYPKYFSSEVVRLIQGLLKRNPKERLGCKNDVEDIKNHEFFKTHDINWSDVYHRRMDPPFKPELKSEMDVSCFDSTFTGQQPIMTPDDCNGAHILPDLFQGFSYVAPNVASSVLNEPMTG---VSRHTRRRNNSGLSGN-----AFNGLNANS 1366
N +K+ E + E+VEICQ VNP +++ E+F + VIG+GGYGKVFLVQK G D+ KLYAMKVLKKAR++ +EKD AHT SERNILEM++HPFLVKLHYAFQN SNLYIVL YC GGELF YLEREG+L+EN ACFY +EI LAIGHLH GIIYRDLK ENVLLD++GHVKLTDFGLSKE V TNTFCGTIEYMAPEI++ GHGKEVDWWSLGTLLFDMLSG PPFSQE ++ T KIL + + +P FSSEV+ LI+GLLKR+P ERLG + D+E+IK H+FFK H+INWSDVY+RR+ PPF+P+L +E DVS FD TFT +P+++P D A I PD+FQGFSYVAP+V L + SRH R +S F LNA+S
Sbjct: 46 NSSVKKNEIKNQETVEICQKTVNPNNRVNAENFNIQRVIGKGGYGKVFLVQKVD-GVDQGKLYAMKVLKKARLVCSEKDKAHTVSERNILEMLQHPFLVKLHYAFQNHSNLYIVLEYCHGGELFNYLEREGSLLENAACFYASEIVLAIGHLHSLGIIYRDLKSENVLLDRQGHVKLTDFGLSKEGVCTTNTFCGTIEYMAPEIIRCEGHGKEVDWWSLGTLLFDMLSGAPPFSQEESREATTLKILTAPLKFPPSFSSEVISLIRGLLKRDPNERLGSREDMEEIKRHKFFKRHEINWSDVYNRRLKPPFRPKLTAENDVSMFDPTFTRLRPVVSPVD-GVASIPPDMFQGFSYVAPSVCEDSLRDNTWTDNLPSRHKRHGLRGAMSAGRLRRIGFGHLNASS 447
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Cavefish
Match: rps6kb1b (ribosomal protein S6 kinase B1 [Source:NCBI gene;Acc:103035647]) HSP 1 Score: 409.838 bits (1052), Expect = 2.895e-136 Identity = 200/360 (55.56%), Postives = 262/360 (72.78%), Query Frame = 2
Query: 206 DDIESVEICQSVVNPGVK-ISMEDFKVLNVIGRGGYGKVFLVQKSSSGHDENKLYAMKVLKKARIIRNEKDTAHTKSERNILEMIRHPFLVKLHYAFQNQSNLYIVLTYCSGGELFTYLEREGTLMENQACFYVAEISLAIGHLHKTGIIYRDLKPENVLLDKKGHVKLTDFGLSKESV---SLTNTFCGTIEYMAPEILKRGGHGKEVDWWSLGTLLFDMLSGGPPFSQECDKQETITKILHSTIAYPKYFSSEVVRLIQGLLKRNPKERLGC-KNDVEDIKNHEFFKTHDINWSDVYHRRMDPPFKPELKSEMDVSCFDSTFTGQQPIMTPDDCNGAHILPDLFQGFSYVAPNVASSV 1270
DD E +EI + VN G + I E F++L V+G+GGYGKVF V+K + G K++AMKVLKKA I+RN KDTAHTK+ERNILE ++HPF+V L YAFQ LY++L Y SGGELF LEREG ME+ ACFY+AEIS+A+GHLH+ GIIYRDLKPEN++L+ +GHVKLTDFGL KES+ ++T+TFCGTIEYMAPEIL R GH + VDWWSLG L++DML+G PPF+ E ++++TI KIL + P Y + E L++ LLKR+ RLG D +++ H FF+ INW D+ R+++PPFKP L+S DVS FDS FT Q P+ +PDD + +F GF+YVAP+V ++
Sbjct: 38 DDCEKIEISEDSVNQGTESIRPECFELLRVLGKGGYGKVFQVRKVTGG-GSGKIFAMKVLKKAMIVRNAKDTAHTKAERNILEEVKHPFIVDLIYAFQTGGKLYLILEYLSGGELFMQLEREGIFMEDTACFYLAEISMALGHLHQKGIIYRDLKPENIMLNNQGHVKLTDFGLCKESIHDGTVTHTFCGTIEYMAPEILMRSGHNRAVDWWSLGALMYDMLTGAPPFTGE-NRKKTIDKILKCKLNLPPYLTQEARDLLKRLLKRSASSRLGAGPGDATEVQTHPFFR--HINWDDLLARKVEPPFKPFLQSAEDVSQFDSKFTSQTPVDSPDDSTLSESANQVFLGFTYVAPSVLENI 393
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Cavefish
Match: rps6kb1b (ribosomal protein S6 kinase B1 [Source:NCBI gene;Acc:103035647]) HSP 1 Score: 408.683 bits (1049), Expect = 8.505e-136 Identity = 200/360 (55.56%), Postives = 262/360 (72.78%), Query Frame = 2
Query: 206 DDIESVEICQSVVNPGVK-ISMEDFKVLNVIGRGGYGKVFLVQKSSSGHDENKLYAMKVLKKARIIRNEKDTAHTKSERNILEMIRHPFLVKLHYAFQNQSNLYIVLTYCSGGELFTYLEREGTLMENQACFYVAEISLAIGHLHKTGIIYRDLKPENVLLDKKGHVKLTDFGLSKESV---SLTNTFCGTIEYMAPEILKRGGHGKEVDWWSLGTLLFDMLSGGPPFSQECDKQETITKILHSTIAYPKYFSSEVVRLIQGLLKRNPKERLGC-KNDVEDIKNHEFFKTHDINWSDVYHRRMDPPFKPELKSEMDVSCFDSTFTGQQPIMTPDDCNGAHILPDLFQGFSYVAPNVASSV 1270
DD E +EI + VN G + I E F++L V+G+GGYGKVF V+K + G K++AMKVLKKA I+RN KDTAHTK+ERNILE ++HPF+V L YAFQ LY++L Y SGGELF LEREG ME+ ACFY+AEIS+A+GHLH+ GIIYRDLKPEN++L+ +GHVKLTDFGL KES+ ++T+TFCGTIEYMAPEIL R GH + VDWWSLG L++DML+G PPF+ E ++++TI KIL + P Y + E L++ LLKR+ RLG D +++ H FF+ INW D+ R+++PPFKP L+S DVS FDS FT Q P+ +PDD + +F GF+YVAP+V ++
Sbjct: 41 DDCEKIEISEDSVNQGTESIRPECFELLRVLGKGGYGKVFQVRKVTGG-GSGKIFAMKVLKKAMIVRNAKDTAHTKAERNILEEVKHPFIVDLIYAFQTGGKLYLILEYLSGGELFMQLEREGIFMEDTACFYLAEISMALGHLHQKGIIYRDLKPENIMLNNQGHVKLTDFGLCKESIHDGTVTHTFCGTIEYMAPEILMRSGHNRAVDWWSLGALMYDMLTGAPPFTGE-NRKKTIDKILKCKLNLPPYLTQEARDLLKRLLKRSASSRLGAGPGDATEVQTHPFFR--HINWDDLLARKVEPPFKPFLQSAEDVSQFDSKFTSQTPVDSPDDSTLSESANQVFLGFTYVAPSVLENI 396
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Cavefish
Match: ENSAMXT00000004157.2 (ribosomal protein S6 kinase B2 [Source:NCBI gene;Acc:103031203]) HSP 1 Score: 405.986 bits (1042), Expect = 3.373e-135 Identity = 208/407 (51.11%), Postives = 277/407 (68.06%), Query Frame = 2
Query: 131 IRLSERDELSIQQFIQNGPLKREEDDDIESVEICQSVVN-PGVKISMEDFKVLNVIGRGGYGKVFLVQKSSSGHDENKLYAMKVLKKARIIRNEKDTAHTKSERNILEMIRHPFLVKLHYAFQNQSNLYIVLTYCSGGELFTYLEREGTLMENQACFYVAEISLAIGHLHKTGIIYRDLKPENVLLDKKGHVKLTDFGLSKESV---SLTNTFCGTIEYMAPEILKRGGHGKEVDWWSLGTLLFDMLSGGPPFSQECDKQETITKILHSTIAYPKYFSSEVVRLIQGLLKRNPKERLGC-KNDVEDIKNHEFFKTHDINWSDVYHRRMDPPFKPELKSEMDVSCFDSTFTGQQPIMTPDDCNGAHILPDLFQGFSYVAPNVASSVLN----EPMTGVSRHTRRRNNS 1324
I L D + + N + E + E VE+ VVN ++ + F++L V+G+GGYGKVF V+K G K++AMKVL+KA+I+RN KDTAHT++ER ILE +RHPF+V L YAFQ LY++L SGGELF LE+EG ME+ ACFY+AEI+LA+GHLH GIIYRDLKPEN++L+ +GH+KLTDFGL KES+ ++T+TFCGTIEYMAPEIL R GH + VDWWSLG L+FDM++G PPF+ E ++++TI KIL I P Y + + LI+ LLK+NP +RLG K D DI+ H FF+ INW D+ H+R++PP++P L+S+ DVS FDS FT Q P+ +PDD + F GF+YVAP+V S+ EP R RR N+S
Sbjct: 7 IDLESEDVSDPEDEVYNFTVAESEPLETEEVELTSEVVNRDSERVGPDCFELLTVLGKGGYGKVFQVRKVQ-GSQMGKIFAMKVLRKAKIVRNAKDTAHTRAEREILETVRHPFIVDLWYAFQTGGKLYLILECLSGGELFMQLEKEGIFMEDTACFYLAEITLALGHLHSNGIIYRDLKPENIMLNHEGHIKLTDFGLCKESIHDGTVTHTFCGTIEYMAPEILNRSGHNRAVDWWSLGALMFDMMTGSPPFTGE-NRKKTIDKILKCKICLPPYLTPDARDLIKKLLKKNPSQRLGSGKGDCADIQKHPFFR--HINWDDLLHKRVEPPYRPSLQSDEDVSQFDSRFTRQTPVDSPDDTTLSGSADHAFAGFTYVAPSVLESLKEGFSVEPR---PRPVRRLNSS 406
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Cavefish
Match: rps6ka4 (ribosomal protein S6 kinase alpha-4-like [Source:NCBI gene;Acc:103040978]) HSP 1 Score: 340.887 bits (873), Expect = 3.802e-106 Identity = 174/344 (50.58%), Postives = 235/344 (68.31%), Query Frame = 2
Query: 257 KISMEDFKVLNVIGRGGYGKVFLVQKSSSGHDENKLYAMKVLKKARIIRNEKDTAHTKSERNILEMIRH-PFLVKLHYAFQNQSNLYIVLTYCSGGELFTYLEREGTLMENQACFYVAEISLAIGHLHKTGIIYRDLKPENVLLDKKGHVKLTDFGLSKESV----SLTNTFCGTIEYMAPEILK-RGGHGKEVDWWSLGTLLFDMLSGGPPFSQECDK---QETITKILHSTIAYPKYFSSEVVRLIQGLLKRNPKERLGCK-NDVEDIKNHEFFKTHDINWSDVYHRRMDPPFKPELKSEMDVSCFDSTFTGQQPIMTPDDCNGAHILPDLFQGFSYVAPNV 1258
K+ ME+F++L V+G G YGKVFLV+K S GHDE KLYAMKVLKKA I++ K HT++ER +LE IR PFLV LHYAFQ Q+ L+++L Y SGGE+FT+L + E++ YV EI LA+ HLHK GI+YRD+K EN+LLD +GHV LTDFGLSKE + T +FCGTIEYMAPEI++ + GHGK VDWWSLG L+F++L+G PF+ E ++ E +IL +P LI+ LL ++P +RLG EDIK+H FFK +NW+D+ +++ PFKPEL++E+DV F FTG +P+ +P + LFQG+S+VAP++
Sbjct: 43 KVGMENFELLKVLGTGAYGKVFLVRKIS-GHDEGKLYAMKVLKKAAIVQKAKTAEHTRTERQVLEHIRQSPFLVTLHYAFQTQTKLHLILDYVSGGEMFTHLYQRDHFSEDEVRIYVGEIILALEHLHKLGIVYRDIKLENILLDSEGHVVLTDFGLSKEFLEEEKERTYSFCGTIEYMAPEIIRGKAGHGKSVDWWSLGILMFELLTGASPFTLEGERNSQSEVSKRILRCEPPFPSLIGPLAQDLIKKLLVKDPHKRLGSGPRGAEDIKSHPFFK--GLNWADLAEKKVASPFKPELRNELDVGNFAEEFTGMEPVYSPASTPPS--TDRLFQGYSFVAPSI 381 HSP 2 Score: 101.679 bits (252), Expect = 5.933e-22 Identity = 72/257 (28.02%), Postives = 123/257 (47.86%), Query Frame = 2
Query: 293 IGRGGYGKVFLVQKSSSGHDENKLYAMKVLKKARIIRNEKDTAHTKSERNILEMIRHPFLVKLHYAFQNQSNLYIVLTYCSGGELFTYLEREGTLMENQACFYVAEISLAIGHLHKTGIIYRDLKPENVLLDKKGH---VKLTDFGLSK-----ESVSLTNTFCGTIEYMAPEILKRGGHGKEVDWWSLGTLLFDMLSGGPPFSQE------CDKQETITKILHSTIAYP----KYFSSEVVRLIQGLLKRNPKERL 1009
+G G + + SG + YA+K++ + +++ A + + HP +V LH + +Q + Y+V+ GGEL L+++ E +A + + A+ +H+ G+++RDLKPENVL + +K+ DFG ++ + T C T++Y APE+ G+ + D WSLG +L+ MLSG PF E + + KI + K S E L++GLL +P+ RL
Sbjct: 431 LGEGSFSICRRCRHKQSGQE----YAVKIISRRMEAMTQREIAALR------QCGSHPNIVTLHEVYTDQYHTYLVMELLRGGELLERLKKKKLFAEGEASQLMKSLVSAVSFMHEAGVVHRDLKPENVLFAGEAEDSALKVIDFGFARLFPAGSGSAPLQTPCFTLQYAAPELFHSSGYDQACDLWSLGVILYTMLSGQVPFQSEKKGMTSTHAADIMHKIKEGDFSLDGDAWKGVSEEAKDLVRGLLTVDPERRL 677
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Cavefish
Match: rps6ka2 (ribosomal protein S6 kinase A2 [Source:NCBI gene;Acc:103036630]) HSP 1 Score: 333.569 bits (854), Expect = 5.795e-104 Identity = 173/362 (47.79%), Postives = 234/362 (64.64%), Query Frame = 2
Query: 191 KREEDDDIESVEICQSVVNPGVKISMEDFKVLNVIGRGGYGKVFLVQKSSSGHDENKLYAMKVLKKARIIRNEKDTAHTKSERNILEMIRHPFLVKLHYAFQNQSNLYIVLTYCSGGELFTYLEREGTLMENQACFYVAEISLAIGHLHKTGIIYRDLKPENVLLDKKGHVKLTDFGLSKESV---SLTNTFCGTIEYMAPEILKRGGHGKEVDWWSLGTLLFDMLSGGPPFSQECDKQETITKILHSTIAYPKYFSSEVVRLIQGLLKRNPKERLGCKND-VEDIKNHEFFKTHDINWSDVYHRRMDPPFKPELKSEMDVSCFDSTFTGQQPIMTPD--DCNGAHILPDLFQGFSYVAPNV 1258
K E+D ++ ++I V K F++L V+G+G YGKVFLV+K G D +LYAMKVLKKA + +D +K ER+IL + HPF+VKLHYAFQ + LY++L + GG+LFT L +E E FY+AE++LA+ HLH GIIYRDLKPEN+LLD+ GH+K+TDFGLSKE++ +FCGTIEYMAPE++ R GH + DWWS G L+F+ML+G PF Q D++ET+ IL + + P++ SSEV L++ L KRNP RLG D VE+IK H FF T I+W+ +Y + + PPFKP + D FD FT + P +P AH LF+GFS+VA N+
Sbjct: 31 KLEDDSILKEIDISNHVKEGFEKADPSQFELLKVLGQGSYGKVFLVRKIK-GSDTGQLYAMKVLKKATL--KVRDRVRSKMERDILAEVNHPFIVKLHYAFQTEGKLYLILDFLRGGDLFTRLSKEVMFTEEDVKFYLAELALALDHLHSLGIIYRDLKPENILLDEDGHIKITDFGLSKEAIDHDKRAYSFCGTIEYMAPEVVNRRGHTQSADWWSFGVLMFEMLTGSLPF-QGKDRKETMALILKAKLGMPQFLSSEVQSLLRALFKRNPSNRLGAGPDGVEEIKRHHFFAT--IDWNKLYRKEIKPPFKPAVGRPEDTFHFDPEFTSRTPTDSPGVPPSANAH---QLFRGFSFVATNL 383 HSP 2 Score: 102.834 bits (255), Expect = 2.820e-22 Identity = 70/231 (30.30%), Postives = 122/231 (52.81%), Query Frame = 2
Query: 365 YAMKVLKKARIIRNEKDTAHTKSERNILEMIRHPFLVKLHYAFQNQSNLYIVLTYCSGGELFTYLEREGTLMENQACFYVAEISLAIGHLHKTGIIYRDLKPENVL-LDKKG---HVKLTDFGLSKESVS---LTNTFCGTIEYMAPEILKRGGHGKEVDWWSLGTLLFDMLSGGPPFSQECDKQETITKILHSTIAYPKYF---------SSEVVRLIQGLLKRNPKERL 1009
YA+K+++K++ KD ++ +L +HP ++ L + + +Y+V+ GGEL + R+ E +A + I+ + +LH G+++RDLKP N+L +D+ G +++ DFG +K+ + L T C T ++APE+LK+ G+ D WSLG LL+ ML+G PF+ D +T +IL + I KY S ++ +L +P +RL
Sbjct: 441 YAVKIIEKSK-----KDP--SEEIEILLRYGQHPNIITLKDVYDDGKFVYLVMELMRGGELLDRILRQKGFSEREASAVLCTITKTVEYLHSQGVVHRDLKPSNILYVDETGDPESIRICDFGFAKQLRAENGLLMTPCYTANFVAPEVLKKQGYDAACDIWSLGILLYTMLAGFTPFANGPD--DTPEEIL-ARIGSGKYILSGGNWDTVSDAAKDIVTKMLHVDPHQRL 661
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Sea Lamprey
Match: ENSPMAT00000006766.1 (pep scaffold:Pmarinus_7.0:GL478342:9725:27922:-1 gene:ENSPMAG00000006086.1 transcript:ENSPMAT00000006766.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 285.419 bits (729), Expect = 7.465e-90 Identity = 150/336 (44.64%), Postives = 211/336 (62.80%), Query Frame = 2
Query: 272 DFKVLNVIGRGGYGKVFLVQKSSSGHDENKLYAMKVLKKARIIRNEKDTAHTKSERNIL-EMIRHPFLVKLHYAFQNQSNLYIVLTYCSGGELFTYLEREGTLMENQACFYVAEISLAIGHLHKTGIIYRDLKPENVLLDKKGHVKLTDFGLSKESV---SLTNTFCGTIEYMAPEILKRGGHGKEVDWWSLGTLLFDMLSGGPPFSQECDKQETITKILHSTIAYPKYFSSEVVRLIQGLLKRNPKERLGCKNDVEDIKNHEFFKTHDINWSDVYHRRMDPPFKPELKSEMDVSCFDSTFTGQQPIM-------TPDDCNGAHILPDLFQGFSYV 1246
DF L V+G+G +GKV L + S G YA+KV+KK+ II +K+ H +ERN+L + +RHPFLV LHY+FQ+ LY V+ Y +GGELF +L+R+G+ E++A FY AEI AIG+LH I+YRDLKPEN+LLD +GH+ LTDFGL KE + S T TFCGT EY+APE+LK+ + + VDWW LG +L++ML G PPF + KQ ILH + ++ + +++GLL ++P +RLG +D+ +IKNH FF T INW+D+ +R+ P+ P + D+ FD FT + M T C D F GF+Y+
Sbjct: 91 DFDFLTVLGKGSFGKVLLAKHKSDG----IFYAVKVMKKS-IILQKKEEKHIMAERNVLVKNVRHPFLVGLHYSFQSADKLYFVMDYVNGGELFFHLQRQGSFPESRAQFYAAEIGSAIGYLHSLNIVYRDLKPENILLDYQGHIVLTDFGLCKEGIDPHSTTCTFCGTPEYLAPEVLKKHPYDRTVDWWCLGCVLYEMLYGLPPFYSKNLKQ-MYESILHQPLQLRPCTTNAAISVLEGLLHKDPHQRLGATSDILEIKNHVFFST--INWNDLDAKRIPSPYNPGVSGPCDLHNFDPAFTAEPLSMSMAQSSDTSSVCANVDEAADAFVGFTYM 418
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Sea Lamprey
Match: ENSPMAT00000008480.1 (pep scaffold:Pmarinus_7.0:GL482122:191709:206473:1 gene:ENSPMAG00000007654.1 transcript:ENSPMAT00000008480.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 276.944 bits (707), Expect = 6.345e-86 Identity = 142/336 (42.26%), Postives = 212/336 (63.10%), Query Frame = 2
Query: 206 DDIESVEICQSVVN-PGVKISMEDFKVLNVIGRGGYGKVFLVQKSSSGHDENKLYAMKVLKKARIIRNEKDTAHTKSERNILEMIRHPFLVKLHYAFQNQSNLYIVLTYCSGGELFTYLEREGTLMENQACFYVAEISLAIGHLH-KTGIIYRDLKPENVLLDKKGHVKLTDFGLSKESV---SLTNTFCGTIEYMAPEILKRGGHGKEVDWWSLGTLLFDMLSGGPPFSQECDKQETITKILHSTIAYPKYFSSEVVRLIQGLLKRNPKERLGCK-NDVEDIKNHEFFKTHDINWSDVYHRRMDPPFKPELKSEMDVSCFDSTFTGQQPIMTPDD 1195
+D S E ++++ P K++M +F L ++G+G +GKV LV++ ++G + YAMK+LKK I+ + + AHT +E +L+ +HPFL L Y+FQ L V+ Y +GGELF +L RE E++ FY AEI A+ +LH + ++YRDLK EN++LDK GH+K+ DFGL KE + + TFCGT EY+APE+L+ +G+ VDWW LG ++++M+ G PF + D ++ IL I +P+ S++ L+ GLLK++PK+RLG +D ++I H FF I W DVY R+M PPFKP++ SE D FD FT Q +TP D
Sbjct: 126 EDGSSTEQMDTILSKPKSKVTMNNFDYLKLLGKGTFGKVILVREKATG----QYYAMKILKKEVIVAKD-EVAHTLTENRVLQTTKHPFLTLLKYSFQTHDRLCFVMEYVNGGELFFHLSRERVFSEDRTRFYGAEIVSALDYLHTEKNVVYRDLKLENLMLDKDGHIKIADFGLCKEGITDGATMKTFCGTPEYLAPEVLEDNDYGRAVDWWGLGVVMYEMMCGRLPFYNQ-DHEKLFELILMEDIRFPRTLSNDARALLSGLLKKDPKQRLGGGVDDAKEIMRHSFFA--GIIWQDVYDRKMTPPFKPQVASETDTRYFDEEFTAQTITITPPD 453
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Sea Lamprey
Match: ENSPMAT00000001814.1 (pep scaffold:Pmarinus_7.0:GL481074:830:17391:1 gene:ENSPMAG00000001637.1 transcript:ENSPMAT00000001814.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 234.958 bits (598), Expect = 1.632e-72 Identity = 117/278 (42.09%), Postives = 178/278 (64.03%), Query Frame = 2
Query: 368 AMKVLKKARIIRNEKDTAHTKSERNILEMIRHPFLVKLHYAFQNQSNLYIVLTYCSGGELFTYLEREGTLMENQACFYVAEISLAIGHLHKTGIIYRDLKPENVLLDKKGHVKLTDFGLSKESVSLTNTFCGTIEYMAPEILKRGGHGKEVDWWSLGTLLFDMLSGGPPFSQECDKQETITKILHSTIAYPKYFSSEVVRLIQGLLKRNPKERLG-CKNDVEDIKNHEFFKTHDINWSDVYHRRMDPPFKPELKSEMDVSCFDSTFTGQQPIMTPDDC 1198
++K K+ ++++ K HT +E+ IL+ I PFLV+L Y+F++ SNLY+VL Y GGE+F++L R G E + FY A+I LA +LH +IYRDLKPEN+L+D++G++++TDFG +K T T CGT EY+APEI+ G+ K VDWW+LG L+++M +G PPF + Q KI+ + +P +FSS++ L++ LL+ + +R G KN V DIKNH++F T D W +Y R+++ PF P+ K D S FD + I + + C
Sbjct: 8 SLKATKRTKVVK-LKQVEHTLNEKRILQAISFPFLVRLEYSFKDNSNLYMVLEYVPGGEMFSHLRRIGRFSEPHSRFYAAQIVLAFEYLHSLDLIYRDLKPENLLIDQQGYIQVTDFGFAKRVKGRTWTLCGTPEYLAPEIILSKGYNKAVDWWALGVLIYEMAAGYPPFFADQPIQ-IYEKIVSGKVRFPSHFSSDLKDLLRNLLQVDLTKRFGNLKNGVNDIKNHKWFSTTD--WIAIYQRKVEAPFIPKCKGPGDASNFDDYEEEEIRISSTEKC 281
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Sea Lamprey
Match: ENSPMAT00000004683.1 (pep scaffold:Pmarinus_7.0:GL488517:199:5283:1 gene:ENSPMAG00000004265.1 transcript:ENSPMAT00000004683.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 203.371 bits (516), Expect = 4.791e-62 Identity = 93/178 (52.25%), Postives = 123/178 (69.10%), Query Frame = 2
Query: 488 AFQNQSNLYIVLTYCSGGELFTYLEREGTLMENQACFYVAEISLAIGHLHKTGIIYRDLKPENVLLDKKGHVKLTDFGLSKESVSLTN---TFCGTIEYMAPEILKRGGHGKEVDWWSLGTLLFDMLSGGPPFSQECDKQETITKILHSTIAYPKYFSSEVVRLIQGLLKRNPKERLG 1012
AFQ + LY++L + GG+LFT L +E E FY+AE++LA+ HLH GIIYRDLKPEN+LLD+ GH+ LTDFGLSKES+ +FCGT+EYMAPE++ R GH DWWS G L+F+ML+G PF Q D++ET+T IL + + P++ S E L++ L KRNP RLG
Sbjct: 1 AFQTEGKLYLILDFLRGGDLFTRLSKEVMFTEEDVKFYLAELALALDHLHSLGIIYRDLKPENILLDESGHIGLTDFGLSKESIDHEKKAYSFCGTVEYMAPEVVNRKGHTHSADWWSFGVLMFEMLTGTLPF-QGKDRKETMTMILKAKLGMPQFLSGEAQSLLRMLFKRNPGNRLG 177
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Sea Lamprey
Match: PRKCD (protein kinase C delta [Source:HGNC Symbol;Acc:HGNC:9399]) HSP 1 Score: 208.764 bits (530), Expect = 6.521e-60 Identity = 114/294 (38.78%), Postives = 170/294 (57.82%), Query Frame = 2
Query: 257 KISMEDFKVLNVIGRGGYGKVFLVQKSSSGHDENKLYAMKVLKKARIIRNEKDTAHTKSERNILEMIRHPFLVKLHYAFQNQSNLYIVLTYCSGGELFTYLEREGT--LMENQACFYVAEISLAIGHLHKTGIIYRDLKPENVLLDKKGHVKLTDFGLSKESVS---LTNTFCGTIEYMAPEILKRGGHGKEVDWWSLGTLLFDMLSGGPPFSQECDKQETITKILHSTIAYPKYFSSEVVRLIQGLLKRNPKERLGCKNDVEDIKNHEFFKTHDINWSDVYHRRMDPPFKPEL 1123
K+S++ F +L V+G+G +GKV L + G K +A+K LKK ++ + +R +L HPFL L FQ + +L+ V+ + +GG+L +++ +G L FY AE+ + LH IIYRDLK +NVLLD++GHVK+ DFG+ KE V L TFCGT +Y+APEIL +G VDWWS+G L+++ML G PF D+ E I T YP++ S ++ L +R+ +RLG V DI+ H FFK +I+W + R++ PPFKP++
Sbjct: 246 KLSLDHFHLLKVLGKGSFGKVMLAELKEQG----KFFAIKALKKEVVLMGDDVECTMVEKRVLLMACEHPFLTHLCGTFQTKEHLFFVMEFLTGGDLMFHIQDKGKFDLPTGTNRFYAAEVICGLQFLHSHNIIYRDLKLDNVLLDREGHVKIADFGMCKEHVGAGNLATTFCGTPDYIAPEILLGRAYGTSVDWWSMGVLIYEMLIGQSPFYGS-DEDELFEAIKGHTPHYPRWLSDSSRNILSCLFERDHTKRLGA---VGDIRLHPFFK--NIDWPSLEARKITPPFKPKV 529
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Yeast
Match: YPK2 (Protein kinase similar to S/T protein kinase Ypk1p; functionally redundant with YPK1 at the genetic level; participates in a signaling pathway required for optimal cell wall integrity; involved in the TORC-dependent phosphorylation of ribosomal proteins Rps6a/b (S6); human homolog SGK2 can complement a ypk1 ypk2 double mutant [Source:SGD;Acc:S000004710]) HSP 1 Score: 273.863 bits (699), Expect = 6.575e-83 Identity = 143/333 (42.94%), Postives = 212/333 (63.66%), Query Frame = 2
Query: 260 ISMEDFKVLNVIGRGGYGKVFLVQKSSSGHDENKLYAMKVLKKARIIRNEKDTAHTKSERNILEMIRHPFLVKLHYAFQNQSNLYIVLTYCSGGELFTYLEREGTLMENQACFYVAEISLAIGHLHKTGIIYRDLKPENVLLDKKGHVKLTDFGLSKESV---SLTNTFCGTIEYMAPEILKRGGHGKEVDWWSLGTLLFDMLSGGPPFSQECDKQETITKILHSTIAYPKYFSSEVVRLIQGLLKRNPKERLGCKNDVEDIKNHEFFKTHDINWSDVYHRRMDPPFKPELKSEMDVSCFDSTFTGQQPIMTPDDCNGAHILPDLFQGFSYVA 1249
+S++DF +L VIG+G +GKV V+K D K+YA+K L+KA I+ ++ + HT +ER +L + PF+V L ++FQ+ LY+VL + +GGELF +L+ EG ++ FY+AE+ A+ LHK +IYRDLKPEN+LLD +GH+ L DFGL K ++ T+TFCGT EY+APEIL G+ K VDWW+LG LL++M++G PP+ E + KIL + +P F L+ GLL R+P RLG N ++I+NH FFK DI+W + + PP+KP +KSE+D + FD FT ++PI + D + + F G++Y+
Sbjct: 339 LSIDDFDLLKVIGKGSFGKVMQVRKK----DTQKIYALKALRKAYIV-SKCEVTHTLAERTVLARVDCPFIVPLKFSFQSPEKLYLVLAFINGGELFYHLQHEGRFSLARSRFYIAELLCALDSLHKLDVIYRDLKPENILLDYQGHIALCDFGLCKLNMKDNDKTDTFCGTPEYLAPEILLGQGYTKTVDWWTLGILLYEMMTGLPPYYDE-NVPVMYKKILQQPLLFPDGFDPAAKDLLIGLLSRDPSRRLGV-NGTDEIRNHPFFK--DISWKKLLLKGYIPPYKPIVKSEIDTANFDQEFTKEKPIDSVVDEYLSASIQKQFGGWTYIG 662
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Yeast
Match: SCH9 (AGC family protein kinase; functional ortholog of mammalian S6 kinase; phosphorylated by Tor1p and required for TORC1-mediated regulation of ribosome biogenesis, translation initiation, and entry into G0 phase; involved in transactivation of osmostress-responsive genes; regulates G1 progression, cAPK activity and nitrogen activation of the FGM pathway; integrates nutrient signals and stress signals from sphingolipids to regulate lifespan [Source:SGD;Acc:S000001248]) HSP 1 Score: 276.174 bits (705), Expect = 1.699e-82 Identity = 151/340 (44.41%), Postives = 212/340 (62.35%), Query Frame = 2
Query: 269 EDFKVLNVIGRGGYGKVFLVQKSSSGHDENKLYAMKVLKKARIIRNEKDTAHTKSERNILEMI---RHPFLVKLHYAFQNQSNLYIVLTYCSGGELFTYLEREGTLMENQACFYVAEISLAIGHLHKTGIIYRDLKPENVLLDKKGHVKLTDFGLSKESV-SLTNTFCGTIEYMAPE-ILKRGGHGKEVDWWSLGTLLFDMLSGGPPFSQECDKQETITKILHSTIAYPK-YFSSEVVRLIQGLLKRNPKERLGCKNDVEDIKNHEFFKTHDINWSDVYHRRMDPPFKPELKSEMDVSCFDSTFT--------GQQPIMTPDDCNGAHILPDLFQGFSYV 1246
+DF+VL ++G+G +G+V+ V+K D ++YAMKVL K ++I + + AHT ERNIL PF+V L ++FQ ++LY+V Y SGGELF +L++EG E++A FY+AE+ LA+ HLH I+YRDLKPEN+LLD G++ L DFGLSK + TNTFCGT EY+APE +L G+ K VD+WSLG L+F+M G PF E + Q+ KI + +P+ S E ++GLL RNPK RLG +D +++ H FF DI+W + +++ PPFKP L SE D S FD FT QP+MT + A + F GF++V
Sbjct: 410 QDFEVLRLLGKGTFGQVYQVKKK----DTQRIYAMKVLSK-KVIVKKNEIAHTIGERNILVTTASKSSPFIVGLKFSFQTPTDLYLVTDYMSGGELFWHLQKEGRFSEDRAKFYIAELVLALEHLHDNDIVYRDLKPENILLDANGNIALCDFGLSKADLKDRTNTFCGTTEYLAPELLLDETGYTKMVDFWSLGVLIFEMCCGWSPFFAE-NNQKMYQKIAFGKVKFPRDVLSQEGRSFVKGLLNRNPKHRLGAIDDGRELRAHPFFA--DIDWEALKQKKIPPPFKPHLVSETDTSNFDPEFTTASTSYMNKHQPMMTATPLSPA--MQAKFAGFTFV 739
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Yeast
Match: YPK1 (S/T protein kinase; phosphorylates, downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p by phosphorylation in response to compromised sphingolipid synthesis; involved in the TORC-dependent phosphorylation of ribosomal proteins Rps6a/b (S6); mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; human homolog SGK1 can complement a null mutant; human homolog SGK2 can complement a ypk1 ypk2 double mutant [Source:SGD;Acc:S000001609]) HSP 1 Score: 270.396 bits (690), Expect = 1.495e-81 Identity = 139/333 (41.74%), Postives = 211/333 (63.36%), Query Frame = 2
Query: 260 ISMEDFKVLNVIGRGGYGKVFLVQKSSSGHDENKLYAMKVLKKARIIRNEKDTAHTKSERNILEMIRHPFLVKLHYAFQNQSNLYIVLTYCSGGELFTYLEREGTLMENQACFYVAEISLAIGHLHKTGIIYRDLKPENVLLDKKGHVKLTDFGLSKESV---SLTNTFCGTIEYMAPEILKRGGHGKEVDWWSLGTLLFDMLSGGPPFSQECDKQETITKILHSTIAYPKYFSSEVVRLIQGLLKRNPKERLGCKNDVEDIKNHEFFKTHDINWSDVYHRRMDPPFKPELKSEMDVSCFDSTFTGQQPIMTPDDCNGAHILPDLFQGFSYVA 1249
+S++DF +L VIG+G +GKV V+K D K+YA+K ++K+ I+ ++ + HT +ER +L + PF+V L ++FQ+ LY VL + +GGELF +L++EG ++A FY AE+ A+ +LHK ++YRDLKPEN+LLD +GH+ L DFGL K ++ T+TFCGT EY+APE+L G+ K VDWW+LG LL++ML+G PP+ E D + KIL + +P F + L+ GLL R+P RLG N ++I+NH FF ++W + + PP+KP + + MD S FD FT ++PI + D + + F G++YV
Sbjct: 342 LSIDDFDLLKVIGKGSFGKVMQVRK----KDTQKVYALKAIRKSYIV-SKSEVTHTLAERTVLARVDCPFIVPLKFSFQSPEKLYFVLAFINGGELFYHLQKEGRFDLSRARFYTAELLCALDNLHKLDVVYRDLKPENILLDYQGHIALCDFGLCKLNMKDDDKTDTFCGTPEYLAPELLLGLGYTKAVDWWTLGVLLYEMLTGLPPYYDE-DVPKMYKKILQEPLVFPDGFDRDAKDLLIGLLSRDPTRRLGY-NGADEIRNHPFFS--QLSWKRLLMKGYIPPYKPAVSNSMDTSNFDEEFTREKPIDSVVDEYLSESVQKQFGGWTYVG 665
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Yeast
Match: TPK1 (cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; inhibited by regulatory subunit Bcy1p in the absence of cAMP; phosphorylates and inhibits Whi3p to promote G1/S phase passage; partially redundant with Tpk2p and Tpk3p; phosphorylates pre-Tom40p, which impairs its import into mitochondria under non-respiratory conditions; TPK1 has a paralog, TPK3, that arose from the whole genome duplication [Source:SGD;Acc:S000003700]) HSP 1 Score: 248.44 bits (633), Expect = 2.285e-76 Identity = 123/311 (39.55%), Postives = 197/311 (63.34%), Query Frame = 2
Query: 227 ICQSVVNPGVKISMEDFKVLNVIGRGGYGKVFLVQKSSSGHDENKLYAMKVLKKARIIRNEKDTAHTKSERNILEMIRHPFLVKLHYAFQNQSNLYIVLTYCSGGELFTYLEREGTLMENQACFYVAEISLAIGHLHKTGIIYRDLKPENVLLDKKGHVKLTDFGLSKESVSLTNTFCGTIEYMAPEILKRGGHGKEVDWWSLGTLLFDMLSGGPPFSQECDKQETITKILHSTIAYPKYFSSEVVRLIQGLLKRNPKERLG-CKNDVEDIKNHEFFKTHDINWSDVYHRRMDPPFKPEL-KSEMDVSCFD 1153
I Q+ V G K S++DF++L +G G +G+V L++ +G + YAMKVLKK ++R K HT ER +L ++ HPF++++ FQ+ +++++ Y GGELF+ L + A FY AE+ LA+ +LH IIYRDLKPEN+LLDK GH+K+TDFG +K +T T CGT +Y+APE++ + K +DWWS G L+++ML+G PF + + +T KIL++ + +P +F+ +V L+ L+ R+ +RLG +N ED+KNH +FK ++ W + R ++ P++P + + + D S FD
Sbjct: 72 IAQARVTSG-KYSLQDFQILRTLGTGSFGRVHLIRSRHNG----RYYAMKVLKKEIVVRL-KQVEHTNDERLMLSIVTHPFIIRMWGTFQDAQQIFMIMDYIEGGELFSLLRKSQRFPNPVAKFYAAEVCLALEYLHSKDIIYRDLKPENILLDKNGHIKITDFGFAKYVPDVTYTLCGTPDYIAPEVVSTKPYNKSIDWWSFGILIYEMLAGYTPFY-DSNTMKTYEKILNAELRFPPFFNEDVKDLLSRLITRDLSQRLGNLQNGTEDVKNHPWFK--EVVWEKLLSRNIETPYEPPIQQGQGDTSQFD 373
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Yeast
Match: TPK2 (cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; partially redundant with Tpk1p and Tpk3p; localizes to P-bodies during stationary phase; relocalizes to the cytosol in response to hypoxia [Source:SGD;Acc:S000006124]) HSP 1 Score: 244.973 bits (624), Expect = 3.446e-75 Identity = 122/310 (39.35%), Postives = 195/310 (62.90%), Query Frame = 2
Query: 233 QSVVNPGVKISMEDFKVLNVIGRGGYGKVFLVQKSSSGHDENKLYAMKVLKKARIIRNEKDTAHTKSERNILEMIRHPFLVKLHYAFQNQSNLYIVLTYCSGGELFTYLEREGTLMENQACFYVAEISLAIGHLHKTGIIYRDLKPENVLLDKKGHVKLTDFGLSKESVSLTNTFCGTIEYMAPEILKRGGHGKEVDWWSLGTLLFDMLSGGPPFSQECDKQETITKILHSTIAYPKYFSSEVVRLIQGLLKRNPKERLG-CKNDVEDIKNHEFFKTHDINWSDVYHRRMDPPFKPELKSEM-DVSCFDS 1156
+S+V+ G K ++ DF+++ +G G +G+V LV+ +G + YA+KVLKK ++++ K HT ER +L+++ HPFL+++ FQ+ N+++V+ Y GGELF+ L + A FY AE+ LA+ +LH IIYRDLKPEN+LLD+ GH+K+TDFG +KE ++T T CGT +Y+APE++ + K VDWWSLG L+++ML+G PF + +T KIL + YP YF +VV L+ L+ + R+G ++ DIK H +F ++ W + + ++ P++P + S + D S FD
Sbjct: 57 KSLVSKG-KYTLHDFQIMRTLGTGSFGRVHLVRSVHNG----RYYAIKVLKKQQVVKM-KQVEHTNDERRMLKLVEHPFLIRMWGTFQDARNIFMVMDYIEGGELFSLLRKSQRFPNPVAKFYAAEVILALEYLHAHNIIYRDLKPENILLDRNGHIKITDFGFAKEVQTVTWTLCGTPDYIAPEVITTKPYNKSVDWWSLGVLIYEMLAGYTPFY-DTTPMKTYEKILQGKVVYPPYFHPDVVDLLSKLITADLTRRIGNLQSGSRDIKAHPWFS--EVVWERLLAKDIETPYEPPITSGIGDTSLFDQ 357
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Nematostella
Match: EDO41166 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S521]) HSP 1 Score: 417.927 bits (1073), Expect = 5.191e-141 Identity = 213/410 (51.95%), Postives = 281/410 (68.54%), Query Frame = 2
Query: 122 NDFIRLSERDELSIQQFIQNGPLKREE---DDDIESVEICQSVVNP-GVKISMEDFKVLNVIGRGGYGKVFLVQKSSSGHDENKLYAMKVLKKARIIRNEKDTAHTKSERNILEMIRHPFLVKLHYAFQNQSNLYIVLTYCSGGELFTYLEREGTLMENQACFYVAEISLAIGHLHKTGIIYRDLKPENVLLDKKGHVKLTDFGLSKESV---SLTNTFCGTIEYMAPEILKRGGHGKEVDWWSLGTLLFDMLSGGPPFSQECDKQETITKILHSTIAYPKYFSSEVVRLIQGLLKRNPKERL-GCKNDVEDIKNHEFFKTHDINWSDVYHRRMDPPFKPELKSEMDVSCFDSTFTGQQPIMTPDDCNGAHILPDLFQGFSYVAPNVASSVLNEPMTGVSRHTRRRNNSG 1327
ND + DELS G + E D+ +E++++ + VNP K+ E F++L V+G+GGYGKVFLV+K+ GH + K++AMKVLKKA IIR++KDTAHTK+ERNILE ++HPF+V L YAFQ LY++L Y SGGELF LEREG +E+ ACFY++EIS+A+ HLHK GI+YRDLKPEN+LLD GHVKLTDFGL KES+ SLT+TFCGTIEYMAPEIL R GHGK VDWWSLG L++DML+G PPF + ++++TI KIL + P Y + E I+ LLKR+P+ RL G D IK H FF+ I W D+ ++++PPF+P + E DVS FDS FT Q P+ +PDD + ++FQGF+YVAP+V S+ + R N+G
Sbjct: 10 NDVALAAGEDELSSDDEFDEGQVYDEPMDGDERMETLQLSEETVNPKDEKVGPESFELLKVLGKGGYGKVFLVKKNH-GHSKEKIFAMKVLKKAAIIRSQKDTAHTKAERNILECVKHPFIVDLIYAFQTGGKLYLILEYLSGGELFMQLEREGIFLEDTACFYLSEISMALEHLHKQGIVYRDLKPENILLDANGHVKLTDFGLCKESIFENSLTHTFCGTIEYMAPEILTRSGHGKAVDWWSLGALMYDMLTGTPPFCAD-NRKKTIDKILKGKLIIPPYLTPEAKDFIRKLLKRHPQARLGGMAGDANPIKAHSFFR--HICWEDLLAKKVEPPFRPSISGEEDVSQFDSKFTRQTPVDSPDDSMLSESASNIFQGFTYVAPSVLESLCRSDNFSPWKVRSPRKNTG 415
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Nematostella
Match: EDO39163 (Ribosomal protein S6 kinase [Source:UniProtKB/TrEMBL;Acc:A7SAZ2]) HSP 1 Score: 320.472 bits (820), Expect = 2.532e-99 Identity = 161/345 (46.67%), Postives = 231/345 (66.96%), Query Frame = 2
Query: 257 KISMEDFKVLNVIGRGGYGKVFLVQKSSSGHDENKLYAMKVLKKARIIRNEKDTAHTKSERNILEMIRH-PFLVKLHYAFQNQSNLYIVLTYCSGGELFTYLEREGTLMENQACFYVAEISLAIGHLHKTGIIYRDLKPENVLLDKKGHVKLTDFGLSKE-----SVSLTNTFCGTIEYMAPEILKRG--GHGKEVDWWSLGTLLFDMLSGGPPFSQECDK--QETITK-ILHSTIAYPKYFSSEVVRLIQGLLKRNPKERLGCKNDVEDIKNHEFFKTHDINWSDVYHRRMDPPFKPELKSEMDVSCFDSTFTGQQPIMTPDDCNGAHILPDLFQGFSYVAPNV 1258
K+ +E+F++L V+G G YGKVFL +K GH + +L+AMKVLKKA I++ K HT++ER +LE +R PFLV LH+AFQ ++ L++++ Y +GGELFT+L + E++ Y+ EI +A+ HLH+ GIIYRD+K EN+LLDK GHV LTDFGLSKE S +FCGTIEYMAPE++K G GH K VDWWSLG L++++L+G PF+ + +K Q I+K ILH+ P S+E + L+ LL+++PK+RLG K ++K H FFK D W+D+ +R+ PFKP ++ E+DVS F FT P +P +F+G+S+VAP++
Sbjct: 10 KVGIENFELLRVLGTGAYGKVFLARKRG-GHHDGRLFAMKVLKKATIVQKAKTAEHTRTERQVLEAVRRCPFLVTLHWAFQTEAKLHLIMDYVNGGELFTHLYQREKFTEDEVRLYIGEIVVALDHLHQLGIIYRDIKLENILLDKDGHVVLTDFGLSKEFAAPNSGERAYSFCGTIEYMAPEVVKGGSRGHDKAVDWWSLGVLMYELLTGASPFTVDGEKNSQSEISKRILHNNPPMPHDLSAEAIDLLHKLLQKDPKKRLGAKG-AHELKAHPFFKAFD--WADLAEKRIPAPFKPRIRDELDVSNFSEEFTDMAPTYSPAAV--PKTADRVFRGYSFVAPSI 348 HSP 2 Score: 112.079 bits (279), Expect = 1.099e-25 Identity = 75/276 (27.17%), Postives = 132/276 (47.83%), Query Frame = 2
Query: 287 NVIGRGGYGKVFLVQKSSSGHDENKLYAMKVLKKARIIRNEKDTAHTKSERNILEMIRHPFLVKLHYAFQNQSNLYIVLTYCSGGELFTYLEREGTLMENQACFYVAEISLAIGHLHKTGIIYRDLKPENVLL---DKKGHVKLTDFGLS--KESVSLTNTFCGTIEYMAPEILKR----GGHGKEVDWWSLGTLLFDMLSGGPPFSQECDKQETITKILHSTIAYP---------KYFSSEVVRLIQGLLKRNPKERLGCKNDVEDIKNHEFFKT 1060
V+G G + SG + YA+K++ + HT+ +++ HP +V L+ +Q++ + Y+V+ +GGEL + ++ E+ A + +I A+ +H+ G+++RDLKPEN+L + +K+ DFG + K T C T+ Y APE+L + G+ + D WSLG +L+ MLSG PF ++ T + I + S + LIQGLL +P +RL V+++ H++ +
Sbjct: 400 QVLGDGSFSTCRRCVHLRSGQE----YAVKIISRR--------CDHTREVQSLKMCHGHPNIVTLYDVYQDEFHTYLVMELLAGGELLERIRKKKMFTESAASVIMRKIVSAVEFMHQRGVVHRDLKPENLLFVDNSEDAEIKIVDFGFACVKPEAQQLQTPCFTLSYAAPEVLDQTVSNSGYDESCDLWSLGVILYTMLSGQVPFQSSGSWYKSSTGFVMQRITQGDVRFDGQQWQSISPQAKDLIQGLLTVDPSQRL----TVQEVLRHDWLRV 659
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Nematostella
Match: EDO31895 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SWM6]) HSP 1 Score: 313.153 bits (801), Expect = 3.731e-98 Identity = 162/331 (48.94%), Postives = 216/331 (65.26%), Query Frame = 2
Query: 275 FKVLNVIGRGGYGKVFLVQKSSSGHDENKLYAMKVLKKARIIRNEKDTAHTKSERNILEMIRHPFLVKLHYAFQNQSNLYIVLTYCSGGELFTYLEREGTLMENQACFYVAEISLAIGHLHKTGIIYRDLKPENVLLDKKGHVKLTDFGLSKESV--SLTNTFCGTIEYMAPEILKRGGHGKEVDWWSLGTLLFDMLSGGPPFSQECDKQETITKILHSTIAYPKYFSSEVVRLIQGLLKRNPKERLGCKN-DVEDIKNHEFFKTHDINWSDVYHRRMDPPFKPELKSEMDVSCFDSTFTGQQPIMTPDDCNGAHILPDLFQGFSYVAPNV 1258
F++L V+G+G +GKVFLV+K G D LYAMKVLKKA + +D T ER+IL ++HPF+V+L+YAFQ + +Y++L + GG+LFT L +E E FY+AE++LA+ HLH GIIYRDLKPEN+LLD GHV LTDFGLSKES T +FCGT+EYMAPE++ R GH + DWWS G L+F+ML+G PF Q D+++T+ IL + + P++ + E L++ L KRNP RLG + +EDIK H FF T INW +Y R + PPFKP D FD FT + P +P A LF+GFSYVAP++
Sbjct: 9 FELLKVLGQGSFGKVFLVRKVI-GADAGTLYAMKVLKKATL--KVRDRMRTMKERDILVDVQHPFIVRLNYAFQTEGKVYLILDFLRGGDLFTRLSKEIMFTEEDVKFYLAELALALDHLHGLGIIYRDLKPENILLDASGHVALTDFGLSKESEHDQKTYSFCGTVEYMAPEVVNRRGHSQAADWWSYGVLMFEMLTGSLPF-QGKDRKDTMNMILKAKLGMPQFLTPEAQSLLRQLFKRNPLNRLGAGDLGIEDIKKHPFFST--INWELLYKREIKPPFKPAAGRADDAFYFDPEFTSKTPQDSPAVPPSA-TCHQLFRGFSYVAPDI 332 HSP 2 Score: 106.301 bits (264), Expect = 5.515e-24 Identity = 67/212 (31.60%), Postives = 112/212 (52.83%), Query Frame = 2
Query: 266 MEDFKVLNVIGRGGYGKVFLVQKSSSGHDENKLYAMKVLKKARIIRNEKDTAHTKSERNILEMIRHPFLVKLHYAFQNQSNLYIVLTYCSGGELFTYLEREGTLMENQACFYVAEISLAIGHLHKTGIIYRDLKPENVLL-DKKG---HVKLTDFGLSKESVS---LTNTFCGTIEYMAPEILKRGGHGKEVDWWSLGTLLFDMLSGGPPFS 880
++++++ IG+G Y + G K YA+K+ +K + KD ++ +L HP +V L A++ +YIV+ GGEL + + L E +A + ++ I LH+ G+++RDLKP N++ D G +++ DFG +K+ + L T C T ++APE+LK+ G+ D WSLG LL+ MLSG PF+
Sbjct: 353 LDEYEIKEEIGKGAYSSCRRCVNKADG----KEYAVKIFEKPK-----KDP--SEEMEILLRFGHHPNIVHLRDAYEAGKQVYIVMELMRGGELLDRILKHKCLSEREAGNIMYTLTSTIAFLHEEGVVHRDLKPSNIMYADDSGTPESLRIVDFGFAKQMRATNGLLMTPCYTANFVAPEVLKKQGYDAACDIWSLGVLLYTMLSGLTPFA 553
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Nematostella
Match: EDO37800 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SEW2]) HSP 1 Score: 290.041 bits (741), Expect = 1.506e-91 Identity = 161/371 (43.40%), Postives = 226/371 (60.92%), Query Frame = 2
Query: 200 EDDDIESVEICQSVVNPGVKISMEDFKVLNVIGRGGYGKVFLVQKSSSGHDENKLYAMKVLKKARIIRNEKDTAHTKSERNIL-EMIRHPFLVKLHYAFQNQSNLYIVLTYCSGGELFTYLEREGTLMENQACFYVAEISLAIGHLHKTGIIYRDLKPENVLLDKKGHVKLTDFGLSKESV---SLTNTFCGTIEYMAPEILKRGGHGKEVDWWSLGTLLFDMLSGGPPFSQECDKQETITKILHSTIAYPKYFSSEVVRLIQGLLKRNPKERLGC-KNDVEDIKNHEFFKTHDINWSDVYHRRMDPPFKPELKSEMDVSCFDSTFTGQQPI--------------MTPDDCNGAHILPDLFQGFSYVAPN 1255
+D D+E +++ +S NP K S DF L VIG+G +GKVFL S E+K YA+KVL K+ IR + H +ERN+L + + HPFLV LHY+FQ + LY VL Y +GGELF +L+RE E++A FY AEI+ AIG+LH IIYRDLKPEN+LLD KGH+ LTDFGL KE + T+TFCGT EY+APE+L++ + K VDWW LG +L++M+ G PPF D E ILH + S +++GLL+++ +RLG + D EDI+ H FF++ INW + R ++PP+ P + ++D+ FD F ++P+ + DD + D F GF+YVAP+
Sbjct: 52 DDADVEKIDLGESN-NPKAKPS--DFLFLKVIGKGSFGKVFL----SKNKQEDKFYAIKVLNKS-AIRKRNEAKHIMAERNVLVQNVTHPFLVGLHYSFQTKDKLYFVLDYVNGGELFFHLQRERYFPESRARFYAAEIASAIGYLHGLQIIYRDLKPENILLDSKGHIVLTDFGLCKEGIDRSGTTSTFCGTPEYLAPEVLRKQDYDKSVDWWCLGAVLYEMMYGLPPFYSR-DTAEMYDNILHKPLRLRTNISQSARHILEGLLQKDKTKRLGSGEGDFEDIRTHSFFRS--INWQQLVARGIEPPYNPNVSGQLDLKHFDPEFV-REPVPGSVGKSADSNFVSASVDDPD------DAFVGFTYVAPS 404
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Nematostella
Match: EDO35585 (Protein kinase C [Source:UniProtKB/TrEMBL;Acc:A7SL27]) HSP 1 Score: 274.633 bits (701), Expect = 2.336e-82 Identity = 140/340 (41.18%), Postives = 221/340 (65.00%), Query Frame = 2
Query: 257 KISMEDFKVLNVIGRGGYGKVFLVQKSSSGHDENKLYAMKVLKKARIIRNEKDTAHTKSERNILEM-IRHPFLVKLHYAFQNQSNLYIVLTYCSGGELFTYLEREGTLMENQACFYVAEISLAIGHLHKTGIIYRDLKPENVLLDKKGHVKLTDFGLSKESVS---LTNTFCGTIEYMAPEILKRGGHGKEVDWWSLGTLLFDMLSGGPPFSQECDKQETITKILHSTIAYPKYFSSEVVRLIQGLLKRNPKERLGC--KNDVEDIKNHEFFKTHDINWSDVYHRRMDPPFKPELKSEMDVSCFDSTFTGQQPIMTPDDCNG-AHILPDLFQGFSYVAPN 1255
K++++DF + V+G+G +GKV L ++ G DE +YA+K+LKK +++++ D T ER +L + HPFL LH +FQ L+ V+ Y +GG+L +++ EN++ FY AE+ LA+ LH+ G+IYRDLK +NVLLD GH+KL DFG+ KE+++ T TFCGT +Y+APEIL+ +G VDWW+LG L+++M++G PPF E ++++ ILH + YP + S E V +++G + +N +RLGC + I+ H FF+ IN+ + R+++PPFKP+++S DV FD+ FT ++P +TP D N +I F+GFS+V P+
Sbjct: 377 KMTLKDFTFIKVLGKGSFGKVLLAERK--GTDE--VYAIKILKKESVLQDD-DVECTMIERRVLALSAGHPFLTSLHSSFQTVERLFFVMEYVNGGDLMFQIQKSRKFDENRSRFYAAEVVLALQFLHQNGVIYRDLKLDNVLLDADGHIKLADFGMCKENMAPGKTTTTFCGTPDYIAPEILQEQPYGFSVDWWALGVLMYEMMAGQPPFEAE-NEEDLFESILHDDVLYPVWLSREAVSILKGFMTKNYLKRLGCTPTSTESSIREHAFFRL--INFEKLEARQINPPFKPKIRSRTDVGNFDTDFTKEEPKLTPTDRNVIVNIDQREFRGFSFVNPH 708
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Medaka
Match: RPS6KB2 (ribosomal protein S6 kinase beta-2 [Source:NCBI gene;Acc:101163545]) HSP 1 Score: 413.305 bits (1061), Expect = 4.395e-138 Identity = 207/388 (53.35%), Postives = 273/388 (70.36%), Query Frame = 2
Query: 188 LKREEDDDIESVEICQSVVN-PGVKISMEDFKVLNVIGRGGYGKVFLVQKSSSGHDENKLYAMKVLKKARIIRNEKDTAHTKSERNILEMIRHPFLVKLHYAFQNQSNLYIVLTYCSGGELFTYLEREGTLMENQACFYVAEISLAIGHLHKTGIIYRDLKPENVLLDKKGHVKLTDFGLSKESV---SLTNTFCGTIEYMAPEILKRGGHGKEVDWWSLGTLLFDMLSGGPPFSQECDKQETITKILHSTIAYPKYFSSEVVRLIQGLLKRNPKERLGC-KNDVEDIKNHEFFKTHDINWSDVYHRRMDPPFKPELKSEMDVSCFDSTFTGQQPIMTPDDCNGAHILPDLFQGFSYVAPNVASSVLN----EPMTGVSRHTRRRNNS 1324
LK+ D E VE+ +VN ++ + F++L V+G+G YGKVF V+K G K++AMKVLKKA+I+RN KDTAHT++ER ILE +RHPF+V L YAFQ LY++L SGGELF LE+EG ME+ ACFY+ EI+LA+GHLH GIIYRDLKPEN++L+ +GH+KLTDFGL KES+ S+T+TFCGTIEYMAPEIL R GH + VDWWSLG L++DM++G PPF+ E ++++TI KIL + P Y + + LI+ LLK+NP +RLG K D DI+ H FFK INW D+ ++R++PP+KP+L SE DVS FD+ FT Q P+ +PDD +H F GF+YVAP+V S+ EP SRH RR N+S
Sbjct: 25 LKKPAADHTEEVELTSEIVNRDSERVGPDCFELLTVLGKGAYGKVFQVRKVQ-GLQIGKIFAMKVLKKAKIVRNAKDTAHTRAEREILETVRHPFIVDLLYAFQTGGKLYLILECLSGGELFMQLEKEGIFMEDTACFYLGEITLALGHLHSNGIIYRDLKPENIMLNHQGHIKLTDFGLCKESIHDGSVTHTFCGTIEYMAPEILTRSGHNRAVDWWSLGALMYDMMTGSPPFTAE-NRKKTIDKILKCKLNLPPYLTIDARELIKKLLKKNPAQRLGSGKADAADIQKHSFFK--QINWDDLLNKRVEPPYKPQLHSEEDVSQFDTRFTRQTPVDSPDDSRLSHSAELAFAGFTYVAPSVLESLKEGFSFEPK---SRHMRRHNSS 405
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Medaka
Match: RPS6KB2 (ribosomal protein S6 kinase beta-2 [Source:NCBI gene;Acc:101163545]) HSP 1 Score: 409.838 bits (1052), Expect = 1.006e-136 Identity = 205/384 (53.39%), Postives = 271/384 (70.57%), Query Frame = 2
Query: 200 EDDDIESVEICQSVVN-PGVKISMEDFKVLNVIGRGGYGKVFLVQKSSSGHDENKLYAMKVLKKARIIRNEKDTAHTKSERNILEMIRHPFLVKLHYAFQNQSNLYIVLTYCSGGELFTYLEREGTLMENQACFYVAEISLAIGHLHKTGIIYRDLKPENVLLDKKGHVKLTDFGLSKESV---SLTNTFCGTIEYMAPEILKRGGHGKEVDWWSLGTLLFDMLSGGPPFSQECDKQETITKILHSTIAYPKYFSSEVVRLIQGLLKRNPKERLGC-KNDVEDIKNHEFFKTHDINWSDVYHRRMDPPFKPELKSEMDVSCFDSTFTGQQPIMTPDDCNGAHILPDLFQGFSYVAPNVASSVLN----EPMTGVSRHTRRRNNS 1324
E+ E VE+ +VN ++ + F++L V+G+G YGKVF V+K G K++AMKVLKKA+I+RN KDTAHT++ER ILE +RHPF+V L YAFQ LY++L SGGELF LE+EG ME+ ACFY+ EI+LA+GHLH GIIYRDLKPEN++L+ +GH+KLTDFGL KES+ S+T+TFCGTIEYMAPEIL R GH + VDWWSLG L++DM++G PPF+ E ++++TI KIL + P Y + + LI+ LLK+NP +RLG K D DI+ H FFK INW D+ ++R++PP+KP+L SE DVS FD+ FT Q P+ +PDD +H F GF+YVAP+V S+ EP SRH RR N+S
Sbjct: 30 ENVQTEEVELTSEIVNRDSERVGPDCFELLTVLGKGAYGKVFQVRKVQ-GLQIGKIFAMKVLKKAKIVRNAKDTAHTRAEREILETVRHPFIVDLLYAFQTGGKLYLILECLSGGELFMQLEKEGIFMEDTACFYLGEITLALGHLHSNGIIYRDLKPENIMLNHQGHIKLTDFGLCKESIHDGSVTHTFCGTIEYMAPEILTRSGHNRAVDWWSLGALMYDMMTGSPPFTAE-NRKKTIDKILKCKLNLPPYLTIDARELIKKLLKKNPAQRLGSGKADAADIQKHSFFK--QINWDDLLNKRVEPPYKPQLHSEEDVSQFDTRFTRQTPVDSPDDSRLSHSAELAFAGFTYVAPSVLESLKEGFSFEPK---SRHMRRHNSS 406
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Medaka
Match: rps6kb1a (ribosomal protein S6 kinase beta-1 [Source:NCBI gene;Acc:101174287]) HSP 1 Score: 407.527 bits (1046), Expect = 1.360e-135 Identity = 202/360 (56.11%), Postives = 262/360 (72.78%), Query Frame = 2
Query: 206 DDIESVEICQSVVNPGV-KISMEDFKVLNVIGRGGYGKVFLVQKSSSGHDENKLYAMKVLKKARIIRNEKDTAHTKSERNILEMIRHPFLVKLHYAFQNQSNLYIVLTYCSGGELFTYLEREGTLMENQACFYVAEISLAIGHLHKTGIIYRDLKPENVLLDKKGHVKLTDFGLSKESV---SLTNTFCGTIEYMAPEILKRGGHGKEVDWWSLGTLLFDMLSGGPPFSQECDKQETITKILHSTIAYPKYFSSEVVRLIQGLLKRNPKERL-GCKNDVEDIKNHEFFKTHDINWSDVYHRRMDPPFKPELKSEMDVSCFDSTFTGQQPIMTPDDCNGAHILPDLFQGFSYVAPNVASSV 1270
DD E E+ ++ VN G +I E F++L V+G+GGYGKVF V+K S G K+YAMKVLKKA I+RN KDTAHTK+ERNILE ++HPF+V L YAFQ LY++L Y SGGELF LEREG ME+ ACFY+AEIS+A+GHLH+ GIIYRDLKPEN++L+ GHVKLTDFGL KES+ ++T+TFCGTIEYMAPEIL R GH + VDWWSLG L++DML+G PPF+ E ++++TI KIL ++ P Y + E L++ LLKR+ RL G D +++ H FF+ INW D+ R+++PPFKP L+S DVS FDS FT Q P+ +PDD + +F GF+YVAP+V +V
Sbjct: 15 DDCEKFELSENNVNKGTEQIRPECFELLKVLGKGGYGKVFQVRKVS-GASSGKIYAMKVLKKAMIVRNAKDTAHTKAERNILEEVKHPFIVDLIYAFQTGGKLYLILEYLSGGELFMQLEREGIFMEDTACFYLAEISMALGHLHQKGIIYRDLKPENIMLNDSGHVKLTDFGLCKESIHDGTVTHTFCGTIEYMAPEILMRSGHNRAVDWWSLGALMYDMLTGAPPFTGE-NRKKTIDKILKCKLSLPPYLTQEARDLLKKLLKRSASSRLGGGPGDAAEVQVHPFFR--HINWDDLLARKVEPPFKPFLQSADDVSQFDSKFTSQTPVDSPDDSTLSESANQVFLGFTYVAPSVLENV 370
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Medaka
Match: rps6kb1a (ribosomal protein S6 kinase beta-1 [Source:NCBI gene;Acc:101174287]) HSP 1 Score: 407.527 bits (1046), Expect = 2.094e-135 Identity = 202/360 (56.11%), Postives = 262/360 (72.78%), Query Frame = 2
Query: 206 DDIESVEICQSVVNPGV-KISMEDFKVLNVIGRGGYGKVFLVQKSSSGHDENKLYAMKVLKKARIIRNEKDTAHTKSERNILEMIRHPFLVKLHYAFQNQSNLYIVLTYCSGGELFTYLEREGTLMENQACFYVAEISLAIGHLHKTGIIYRDLKPENVLLDKKGHVKLTDFGLSKESV---SLTNTFCGTIEYMAPEILKRGGHGKEVDWWSLGTLLFDMLSGGPPFSQECDKQETITKILHSTIAYPKYFSSEVVRLIQGLLKRNPKERL-GCKNDVEDIKNHEFFKTHDINWSDVYHRRMDPPFKPELKSEMDVSCFDSTFTGQQPIMTPDDCNGAHILPDLFQGFSYVAPNVASSV 1270
DD E E+ ++ VN G +I E F++L V+G+GGYGKVF V+K S G K+YAMKVLKKA I+RN KDTAHTK+ERNILE ++HPF+V L YAFQ LY++L Y SGGELF LEREG ME+ ACFY+AEIS+A+GHLH+ GIIYRDLKPEN++L+ GHVKLTDFGL KES+ ++T+TFCGTIEYMAPEIL R GH + VDWWSLG L++DML+G PPF+ E ++++TI KIL ++ P Y + E L++ LLKR+ RL G D +++ H FF+ INW D+ R+++PPFKP L+S DVS FDS FT Q P+ +PDD + +F GF+YVAP+V +V
Sbjct: 25 DDCEKFELSENNVNKGTEQIRPECFELLKVLGKGGYGKVFQVRKVS-GASSGKIYAMKVLKKAMIVRNAKDTAHTKAERNILEEVKHPFIVDLIYAFQTGGKLYLILEYLSGGELFMQLEREGIFMEDTACFYLAEISMALGHLHQKGIIYRDLKPENIMLNDSGHVKLTDFGLCKESIHDGTVTHTFCGTIEYMAPEILMRSGHNRAVDWWSLGALMYDMLTGAPPFTGE-NRKKTIDKILKCKLSLPPYLTQEARDLLKKLLKRSASSRLGGGPGDAAEVQVHPFFR--HINWDDLLARKVEPPFKPFLQSADDVSQFDSKFTSQTPVDSPDDSTLSESANQVFLGFTYVAPSVLENV 380
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Medaka
Match: rps6kb1b (ribosomal protein S6 kinase beta-1-like [Source:NCBI gene;Acc:101174764]) HSP 1 Score: 405.986 bits (1042), Expect = 3.081e-135 Identity = 201/363 (55.37%), Postives = 260/363 (71.63%), Query Frame = 2
Query: 185 PLKREEDDDIESVEICQSVVNPGVK-ISMEDFKVLNVIGRGGYGKVFLVQKSSSGHDENKLYAMKVLKKARIIRNEKDTAHTKSERNILEMIRHPFLVKLHYAFQNQSNLYIVLTYCSGGELFTYLEREGTLMENQACFYVAEISLAIGHLHKTGIIYRDLKPENVLLDKKGHVKLTDFGLSKESV---SLTNTFCGTIEYMAPEILKRGGHGKEVDWWSLGTLLFDMLSGGPPFSQECDKQETITKILHSTIAYPKYFSSEVVRLIQGLLKRNPKERLGC-KNDVEDIKNHEFFKTHDINWSDVYHRRMDPPFKPELKSEMDVSCFDSTFTGQQPIMTPDDCNGAHILPDLFQGFSYVAPNV 1258
P + DD+ E +EI + VN G + I E F++L V+G+GGYGKVF V+K G K++AMKVLKKA I+RN KDTAHTK+ERNILE ++HPF+V L YAFQ LY++L Y SGGELF LEREG ME+ ACFY+AEIS+A+GHLH+ GIIYRDLKPEN++L+ +GHVKLTDFGL KES+ ++T+TFCGTIEYMAPEIL R GH + VDWWSLG L++DML+G PPF+ E ++++TI KIL + P Y + E L++ LLKR RLG D +++ H FF+ INW D+ R+++PPFKP L+S DVS FDS FT Q P+ +PDD + F GF+YVAP+V
Sbjct: 12 PEENVSDDENEEIEISEDNVNQGTESIRPECFELLRVLGKGGYGKVFQVRKVV-GAAAGKIFAMKVLKKAMIVRNAKDTAHTKAERNILEEVKHPFIVDLIYAFQTGGKLYLILEYLSGGELFMQLEREGIFMEDTACFYLAEISMALGHLHQKGIIYRDLKPENIMLNSQGHVKLTDFGLCKESIHDGTVTHTFCGTIEYMAPEILMRSGHNRAVDWWSLGALMYDMLTGAPPFTGE-NRKKTIDKILKCKLNLPPYLTQEARDLLKRLLKRTASSRLGAGPGDATEVQAHPFFR--HINWEDLLARKVEPPFKPVLQSADDVSQFDSKFTSQTPVDSPDDSTLSESANQAFLGFTYVAPSV 370
BLAST of Non-specific serine/threonine protein kinase vs. Planmine SMEST
Match: SMESG000004478.1 (SMESG000004478.1) HSP 1 Score: 1113.21 bits (2878), Expect = 0.000e+0 Identity = 547/547 (100.00%), Postives = 547/547 (100.00%), Query Frame = 2
Query: 68 MESSLDYIVTEDDIVSKDNDFIRLSERDELSIQQFIQNGPLKREEDDDIESVEICQSVVNPGVKISMEDFKVLNVIGRGGYGKVFLVQKSSSGHDENKLYAMKVLKKARIIRNEKDTAHTKSERNILEMIRHPFLVKLHYAFQNQSNLYIVLTYCSGGELFTYLEREGTLMENQACFYVAEISLAIGHLHKTGIIYRDLKPENVLLDKKGHVKLTDFGLSKESVSLTNTFCGTIEYMAPEILKRGGHGKEVDWWSLGTLLFDMLSGGPPFSQECDKQETITKILHSTIAYPKYFSSEVVRLIQGLLKRNPKERLGCKNDVEDIKNHEFFKTHDINWSDVYHRRMDPPFKPELKSEMDVSCFDSTFTGQQPIMTPDDCNGAHILPDLFQGFSYVAPNVASSVLNEPMTGVSRHTRRRNNSGLSGNAFNGLNANSPFLNENDFIEMSCNDAYVNQPVTNALTYFPCTVNNTHFFASQSPYIPSQPSNFQPANISFIQNMYNPMGQFFKWNHNPIISTAPVFXXXXXXXXXXXXXCPIPIPQNESRKIPQ 1708
MESSLDYIVTEDDIVSKDNDFIRLSERDELSIQQFIQNGPLKREEDDDIESVEICQSVVNPGVKISMEDFKVLNVIGRGGYGKVFLVQKSSSGHDENKLYAMKVLKKARIIRNEKDTAHTKSERNILEMIRHPFLVKLHYAFQNQSNLYIVLTYCSGGELFTYLEREGTLMENQACFYVAEISLAIGHLHKTGIIYRDLKPENVLLDKKGHVKLTDFGLSKESVSLTNTFCGTIEYMAPEILKRGGHGKEVDWWSLGTLLFDMLSGGPPFSQECDKQETITKILHSTIAYPKYFSSEVVRLIQGLLKRNPKERLGCKNDVEDIKNHEFFKTHDINWSDVYHRRMDPPFKPELKSEMDVSCFDSTFTGQQPIMTPDDCNGAHILPDLFQGFSYVAPNVASSVLNEPMTGVSRHTRRRNNSGLSGNAFNGLNANSPFLNENDFIEMSCNDAYVNQPVTNALTYFPCTVNNTHFFASQSPYIPSQPSNFQPANISFIQNMYNPMGQFFKWNHNPIISTAPVFTSTPSSTQPNNSTCPIPIPQNESRKIPQ
Sbjct: 1 MESSLDYIVTEDDIVSKDNDFIRLSERDELSIQQFIQNGPLKREEDDDIESVEICQSVVNPGVKISMEDFKVLNVIGRGGYGKVFLVQKSSSGHDENKLYAMKVLKKARIIRNEKDTAHTKSERNILEMIRHPFLVKLHYAFQNQSNLYIVLTYCSGGELFTYLEREGTLMENQACFYVAEISLAIGHLHKTGIIYRDLKPENVLLDKKGHVKLTDFGLSKESVSLTNTFCGTIEYMAPEILKRGGHGKEVDWWSLGTLLFDMLSGGPPFSQECDKQETITKILHSTIAYPKYFSSEVVRLIQGLLKRNPKERLGCKNDVEDIKNHEFFKTHDINWSDVYHRRMDPPFKPELKSEMDVSCFDSTFTGQQPIMTPDDCNGAHILPDLFQGFSYVAPNVASSVLNEPMTGVSRHTRRRNNSGLSGNAFNGLNANSPFLNENDFIEMSCNDAYVNQPVTNALTYFPCTVNNTHFFASQSPYIPSQPSNFQPANISFIQNMYNPMGQFFKWNHNPIISTAPVFTSTPSSTQPNNSTCPIPIPQNESRKIPQ 547
BLAST of Non-specific serine/threonine protein kinase vs. Planmine SMEST
Match: SMESG000046977.1 (SMESG000046977.1) HSP 1 Score: 382.104 bits (980), Expect = 2.851e-127 Identity = 179/235 (76.17%), Postives = 201/235 (85.53%), Query Frame = 2
Query: 578 MENQACFYVAEISLAIGHLHKTGIIYRDLKPENVLLDKKGHVKLTDFGLSKESVSLTNTFCGTIEYMAPEILKRGGHGKEVDWWSLGTLLFDMLSGGPPFSQECDKQETITKILHSTIAYPKYFSSEVVRLIQGLLKRNPKERLGCKNDVEDIKNHEFFKTHDINWSDVYHRRMDPPFKPELKSEMDVSCFDSTFTGQQPIMTPDDCNGAHILPDLFQGFSYVAPNVASSVLNEP 1282
MENQACFY+AEISLAIGHLH GIIYRDLKPENVLLDK GHVKLTDFGLSKES + TNTFCGTIEYMAPEIL+R GH VDWWSLGTLLFDMLSGGPPFSQE KQ TI KIL + + +P YFS +V+ LI+GLLKRNPKERLG KNDVE+IK+H FF+ +DINW D+ R+++PPFKP+LKSE DVSCFDSTFT QQPI++PDD G HI D+F GFSYVAPNVA SVL+EP
Sbjct: 1 MENQACFYIAEISLAIGHLHSKGIIYRDLKPENVLLDKCGHVKLTDFGLSKESATSTNTFCGTIEYMAPEILRREGHTFVVDWWSLGTLLFDMLSGGPPFSQESSKQLTIEKILRAPLKFPMYFSLDVMGLIKGLLKRNPKERLGYKNDVEEIKSHIFFQKNDINWDDILSRKVEPPFKPDLKSEADVSCFDSTFTNQQPILSPDDSTGTHISEDIFTGFSYVAPNVAESVLSEP 235
BLAST of Non-specific serine/threonine protein kinase vs. Planmine SMEST
Match: SMESG000068122.1 (SMESG000068122.1) HSP 1 Score: 360.147 bits (923), Expect = 6.447e-118 Identity = 192/408 (47.06%), Postives = 269/408 (65.93%), Query Frame = 2
Query: 191 KREEDDDIESVEICQSVVNP-GVKISMEDFKVLNVIGRGGYGKVFLVQKSSSGHDENKLYAMKVLKKARIIRNEK-DTAHTKSERNILEMIRHPFLVKLHYAFQNQSNLYIVLTYCSGGELFTYLEREGTLMENQACFYVAEISLAIGHLHKTGIIYRDLKPENVLLDKKGHVKLTDFGLSKE--SVSLTNTFCGTIEYMAPEILKRGGHGKEVDWWSLGTLLFDMLSGGPPFSQECDKQETITKILHSTIAYPKYFSSEVVRLIQGLLKRNPKERL-GCKNDVEDIKNHEFFKTHDINWSDVYHRRMDPPFKPELKSEMDVSCFDSTFTGQQPIMTPDDCNGAHILPDLFQGFSYVAPNVASSVLNEPMTGVSRHTRRRNNSGLSGNAFNGLNANSPFLNENDFIEM 1399
K E D +SV+IC+ VNP GVK++ DF +L +G+GGYG+VFL +K + G + +AMKV+KKAR+I N+K DT + +ERNILE + HPFLVKL++AFQ +L++VL Y +GGELF LER+ TL E++A FY+AE +LA+GHLH G+IYRDLK ENV+LD+ GHVKLTDFGLSKE LT++FCGT+EYMAPEIL+R GHG VDWWS+G LL+DML G PF ++ + I K+ ++ + P++ + L++ LL +NPK RL G + DVE+IK H FF++ INW R + PPF PEL S DVS FD + +++P + ++ F+ FSYV+P V L+E + + TR + S + + + FL E DF+++
Sbjct: 40 KDEYDSTSKSVDICEETVNPPGVKVTSHDFDLLKKLGKGGYGQVFLAKKVN-GAMAGEYFAMKVVKKARLITNKKNDTKYAMTERNILEAVNHPFLVKLYFAFQTNHSLFLVLEYLAGGELFMQLERQHTLTEDEARFYLAETTLALGHLHSIGVIYRDLKSENVMLDRYGHVKLTDFGLSKERNDDELTHSFCGTVEYMAPEILQRRGHGPSVDWWSMGILLYDMLIGNSPFVG-GNRSQVIQKVTNAKLKIPRFLTDSGKHLLKNLLNKNPKARLGGGEKDVEEIKRHFFFRS--INWDKALARELKPPFIPELSSRDDVSLFDKNIVSEPCMISPTTTDDSNCY--WFENFSYVSPQVYLE-LSENGSFSRQRTRSGVSHSSSSPGDSHHHQTTQFLFEEDFVDL 440
BLAST of Non-specific serine/threonine protein kinase vs. Planmine SMEST
Match: SMESG000059433.1 (SMESG000059433.1) HSP 1 Score: 342.428 bits (877), Expect = 1.940e-107 Identity = 178/393 (45.29%), Postives = 254/393 (64.63%), Query Frame = 2
Query: 122 NDFIRLSERDELSI-QQFIQNGPLKREEDDDIESVEICQSVVNPGVKISMEDFKVLNVIGRGGYGKVFLVQKSSSGHDENKLYAMKVLKKARIIRNEKDTAHTKSERNILEMIRHPFLVKLHYAFQNQSNLYIVLTYCSGGELFTYLEREGTLMENQACFYVAEISLAIGHLHKTGIIYRDLKPENVLLDKKGHVKLTDFGLSKESV-----SLTNTFCGTIEYMAPEILKRGGHGKEVDWWSLGTLLFDMLSGGPPFSQECDKQETITKILHSTIAYPKYFSSEVVRLIQGLLKRNPKERLG-CKNDVEDIKNHEFFKTHDINWSDVYHRRMDPPFKPELKSEMDVSCFDSTFTGQQPIMTPDDCNGAHILP-DLFQGFSYVAPNVASSVLN 1276
D +S +E+ + ++F N + EE ++ +V++ K+ F++L V+G+G +GKVFLV+K S + LYAMKVLKKA + +D TK ER+IL IRHPF+V LHYAFQN+ +Y++L + GG+LF+ L +E E+ FY+AE++LA+ HLH GI+YRDLKPEN+LLD+ GH+KLTDFGLSKES+ + T +FCGT+EYMAPE++ R GHG DWWS G L+F+ML+G PF Q ++ET+T IL + ++ P++ SSE L++ L KRNP R+G +E+IK+ FF+ INW D+ +R++ PPFKP+ D SCFDS FT + P +P C A ++F+GFSYVAP+V N
Sbjct: 7 TDSFHISNLNEVEVDEEFSDNAFSQEEEATEVNAVDLVDE------KLDSSHFELLRVLGQGSFGKVFLVRKVSEPQ-QGILYAMKVLKKASL--KVQDRKRTKMERDILAAIRHPFIVTLHYAFQNEGKVYLILEFLKGGDLFSRLSKEFMFTEDDVKFYLAELALALDHLHSLGIVYRDLKPENILLDEDGHIKLTDFGLSKESIYNDDANRTFSFCGTVEYMAPEVVNRKGHGTAADWWSFGVLMFEMLTGQLPF-QGSSRKETMTMILKAKLSMPQFLSSEAQTLLRVLFKRNPSNRIGFGPKGIEEIKSQPFFQL--INWKDLLNRKVTPPFKPKATCLDDTSCFDSQFTSKTPKESP--CQPASATANEIFRGFSYVAPDVLQHAQN 385 HSP 2 Score: 116.316 bits (290), Expect = 9.488e-27 Identity = 88/298 (29.53%), Postives = 150/298 (50.34%), Query Frame = 2
Query: 188 LKREEDDDIESVEIC--QSVVNPGVKISMED--FKVLNVIGRGGYGKVFLVQKSSSGHDENKLYAMKVLKKARIIRNEKDTAHTKSERNILEMIR-------HPFLVKLHYAFQNQSNLYIVLTYCSGGELFTYLEREGTLMENQACFYVAEISLAIGHLHKTGIIYRDLKPENVLLDKKG----HVKLTDFGLSKESVS---LTNTFCGTIEYMAPEILKRGGHGKEVDWWSLGTLLFDMLSGGPPFSQ-ECDKQETITKILHS-----TIAYPKYFSSEVVRLIQGLLKRNPKERL 1009
L+ ++ +IE ++I Q++V GVK D + + IG+G + + ++G + YA+KV+ + R+++E + HP +VKL + Q+ + +++ Y GGEL T + + E +A + ++ + +LH +++RDLKP N+L KK ++ + DFG +K+ + L T C T ++APE+LK+ G+ K D WSLG LLF MLSG PF+Q E D E I K + T ++ SSE L+ +L +P +R+
Sbjct: 380 LQHAQNTNIEDMKISGWQNIV--GVKFQKVDHYYTLKEEIGKGSFSVCYRAVHKTTGVN----YAIKVIDGTK--------------RDVIEEVEILFRFGAHPHIVKLKEVYVQQNTVSLIMEYLEGGELLTNILSQKYFSEKEASKILYVLTETVHYLHTNLVVHRDLKPSNILYSKKDTNPENLVICDFGFAKQQRAENGLLMTPCYTANFVAPEVLKKQGYDKASDVWSLGVLLFIMLSGKTPFTQNEHDPPEEILKRIEKKSFQLTGGQWEHISSEAKDLLLKMLHCDPAQRI 657
BLAST of Non-specific serine/threonine protein kinase vs. Planmine SMEST
Match: SMESG000022857.1 (SMESG000022857.1) HSP 1 Score: 267.314 bits (682), Expect = 9.918e-82 Identity = 141/339 (41.59%), Postives = 210/339 (61.95%), Query Frame = 2
Query: 272 DFKVLNVIGRGGYGKVFLVQKSSSGHDENKLYAMKVLKKARIIRNEKDTAHTKSERNIL-EMIRHPFLVKLHYAFQNQSNLYIVLTYCSGGELFTYLEREGTLMENQACFYVAEISLAIGHLHKTGIIYRDLKPENVLLDKKGHVKLTDFGLSKESVS---LTNTFCGTIEYMAPEILKRGGHGKEVDWWSLGTLLFDMLSGGPPFSQECDKQETITKILHSTIAYPKYFSSEVVRLIQGLLKRNPKERLGCKNDVEDIKNHEFFKTHDINWSDVYHRRMDPPFKPELKSEMDVSCFDSTFTGQQPI--------MTPDDCNGAHILPDLFQGFSYVAP 1252
DF + IG+G +GKVFL ++ S G ++A+KVL K I + + H SERNIL + +RHPFL +L+++FQ + LY L Y +GGELF +L++E + + +A FY AEI ++G+LH IIYRDLKPEN+LLD +GH+ LTDFGL KE + T+TFCGT EY+APE++K+ + + VDWWS G+++++ML G PPF + D + +IL+S + S + ++ L+++NPKERLG KND ++IK H FF I+W + ++++ PPF P++ S D+ D F ++PI + + D F GFSYV P
Sbjct: 142 DFDFIKTIGKGSFGKVFLARRKSDG----AVFAVKVLYKENI-KKRNEVKHIMSERNILVQNLRHPFLCRLYFSFQTKRKLYFFLDYVNGGELFFHLQKERSFSQKRAQFYAAEIGSSLGYLHSKNIIYRDLKPENILLDAQGHIILTDFGLCKEGIEREGTTSTFCGTPEYLAPEVIKKEPYDRSVDWWSFGSVIYEMLFGLPPFYSQ-DTHQMYQRILNSPLPLRGNCPSVISDILVKLIRKNPKERLGYKNDFDEIKVHPFFSQ--IDWVALVNKQVKPPFVPQVSSNADIRNIDPEFH-KEPISGSIPNSQLNRRTQLSVEDVDDSFVGFSYVPP 471 The following BLAST results are available for this feature:
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 5
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 5
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of Non-specific serine/threonine protein kinase vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of Non-specific serine/threonine protein kinase vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 5
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 5
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 5
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of Non-specific serine/threonine protein kinase vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30023085 ID=SMED30023085|Name=Non-specific serine/threonine protein kinase|organism=Schmidtea mediterranea sexual|type=transcript|length=2024bpback to top protein sequence of SMED30023085-orf-1 >SMED30023085-orf-1 ID=SMED30023085-orf-1|Name=SMED30023085-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=548bp MESSLDYIVTEDDIVSKDNDFIRLSERDELSIQQFIQNGPLKREEDDDIEback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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