SMED30023071

Overview
NameSMED30023071
Smed IDSMED30023071
Length (bp)2401
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30023071 (SMED30023071) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30023071 (SMED30023071) in cells (dots) of each of the 12 neoblast clusters.

 

back to top

 

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30023071 (SMED30023071) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30023071 (SMED30023071) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

back to top

 

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

back to top
Anatomical Expression

PAGE et. al., 2020



SMED30023071

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 6

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30023071SMESG000054475.1 SmedASXL_010481SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
nervous systemSMED30023071SMESG000054475.1 dd_Smed_v4_10308_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30023071SMESG000054475.1 dd_Smed_v4_10308_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30023071SMESG000054475.1 dd_Smed_v4_10308_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30023071SMESG000054475.1 dd_Smed_v4_10308_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30023071SMESG000054475.1 dd_Smed_v4_10308_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30023071 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.007107:3197..16440 -11822
Homology
[Close]
BLAST of SMED30023071 vs. Planmine SMEST
Match: SMESG000054475.1 (SMESG000054475.1)

HSP 1 Score: 1354.73 bits (3505), Expect = 0.000e+0
Identity = 707/709 (99.72%), Postives = 709/709 (100.00%), Query Frame = 1 
Query:   91 MICHFKSHIQWSAWVSVIFIGILQVYVVYSSLSRYIMLKDETFHGYHMHFAEYRWXXXXXXXXXXXXXGALGVLPIWFYSAMFSISSFFCKGFSPQLNRQGFKELVLKEAIEVFSQQKLEVNNSLHQDHPRNCSCQKTIRFGSFYPIASIFQVLIAYXXXXXXXFIESWLIQIDHKHKGGIQTNDAEFLVSNGLLLKLNWNVEYFNFLISLIGLSLQFPNFIWGANKLFSIAXXXXXXXXXXXXXXXXXXXSIVIKFLFKNCTACEDGTESVVVYSTILLISSVIGNLFLLLTFFSIFQFAHEKCVQSLLKSSWFFNNYRDDNVSFSTNLDQVPGQSNAMSSTLYSLKKIREVKTSLYPFTSAALFFVSGIALKCFTYYHFQEFRNTTKIFIIYFIQSAMLVFVYFLFWFGISLKPKWSFSIKDLENRTTIHSPIFQSEEANYYKSLKSNIKTSLISLSSASNTNLSVHSTQVQSPNTRIDFTSPDPYNNKCNSLKFPVQSRENQDFSDYIQLSQYLPQPRFYKTMYTSISSPKPFTENRDGKLMLNTFYKCNNSNSLGKPAENSRHEDPYQMIKKSNMEFSIQEETSNENVSSISDDFSSNMTSSPNNDLKNKQNPNMKFVTFRLDNAEITSGLSPSTSNESLTVITLEQSEAFLKELEGDAPDKIHFNKFRKEINHDFRDKFILPSAPTHCEADSFPLASHTKASLL 2217
            MICHFKSHIQWSAWVSVIFIGILQVYVVYSSLSRYIMLKDETFHGYHMHFAEYRWIFFLLNFQLFIIIGALGVLPIWFYSAMFSISSFFCKGFSPQLNRQGFKELVLKEAIEVFSQQKLEVNNSLHQDHPRNCSCQKTIRFGSFYPIASIFQVLIAYLLLLPLPFIESWLIQIDHKHKGGIQTNDAEFLVSNGLLLKLNWNVEYFNFLISLIGLSLQFPNFIWGANKLFSIAFSLLEFIIIFHILIDYPIISIVIKFLFKNCTACEDGTESVVVYSTILLISSVIGNLFLLLTFFSIFQFAHEKCVQSLLKSSWFFNNYRDDNVSFSTNLDQVPGQSNAMSSTLYSL+KIREVKTSLYPFTSAALFFVSGIALKCFTYYHFQEFRNTTKIFIIYFIQSAMLVFVYFLFWFGISLKPKWSFSIKDLENRTTIHSPIFQSEEANYYKSLKSNIKTSLISLSSASNTNLSVHSTQVQSPNTRIDFTSPDPYNNKCNSLKFPVQSRENQDFSDYIQL+QYLPQPRFYKTMYTSISSPKPFTENRDGKLMLNTFYKCNNSNSLGKPAENSRHEDPYQMIKKSNMEFSIQEETSNENVSSISDDFSSNMTSSPNNDLKNKQNPNMKFVTFRLDNAEITSGLSPSTSNESLTVITLEQSEAFLKELEGDAPDKIHFNKFRKEINHDFRDKFILPSAPTHCEADSFPLASHTKASLL
Sbjct:    1 MICHFKSHIQWSAWVSVIFIGILQVYVVYSSLSRYIMLKDETFHGYHMHFAEYRWIFFLLNFQLFIIIGALGVLPIWFYSAMFSISSFFCKGFSPQLNRQGFKELVLKEAIEVFSQQKLEVNNSLHQDHPRNCSCQKTIRFGSFYPIASIFQVLIAYLLLLPLPFIESWLIQIDHKHKGGIQTNDAEFLVSNGLLLKLNWNVEYFNFLISLIGLSLQFPNFIWGANKLFSIAFSLLEFIIIFHILIDYPIISIVIKFLFKNCTACEDGTESVVVYSTILLISSVIGNLFLLLTFFSIFQFAHEKCVQSLLKSSWFFNNYRDDNVSFSTNLDQVPGQSNAMSSTLYSLEKIREVKTSLYPFTSAALFFVSGIALKCFTYYHFQEFRNTTKIFIIYFIQSAMLVFVYFLFWFGISLKPKWSFSIKDLENRTTIHSPIFQSEEANYYKSLKSNIKTSLISLSSASNTNLSVHSTQVQSPNTRIDFTSPDPYNNKCNSLKFPVQSRENQDFSDYIQLNQYLPQPRFYKTMYTSISSPKPFTENRDGKLMLNTFYKCNNSNSLGKPAENSRHEDPYQMIKKSNMEFSIQEETSNENVSSISDDFSSNMTSSPNNDLKNKQNPNMKFVTFRLDNAEITSGLSPSTSNESLTVITLEQSEAFLKELEGDAPDKIHFNKFRKEINHDFRDKFILPSAPTHCEADSFPLASHTKASLL 709
[Close]
BLAST of SMED30023071 vs. Planmine SMEST
Match: SMESG000054475.1 (SMESG000054475.1)

HSP 1 Score: 1343.95 bits (3477), Expect = 0.000e+0
Identity = 702/704 (99.72%), Postives = 704/704 (100.00%), Query Frame = 1 
Query:  106 KSHIQWSAWVSVIFIGILQVYVVYSSLSRYIMLKDETFHGYHMHFAEYRWXXXXXXXXXXXXXGALGVLPIWFYSAMFSISSFFCKGFSPQLNRQGFKELVLKEAIEVFSQQKLEVNNSLHQDHPRNCSCQKTIRFGSFYPIASIFQVLIAYXXXXXXXFIESWLIQIDHKHKGGIQTNDAEFLVSNGLLLKLNWNVEYFNFLISLIGLSLQFPNFIWGANKLFSIAXXXXXXXXXXXXXXXXXXXSIVIKFLFKNCTACEDGTESVVVYSTILLISSVIGNLFLLLTFFSIFQFAHEKCVQSLLKSSWFFNNYRDDNVSFSTNLDQVPGQSNAMSSTLYSLKKIREVKTSLYPFTSAALFFVSGIALKCFTYYHFQEFRNTTKIFIIYFIQSAMLVFVYFLFWFGISLKPKWSFSIKDLENRTTIHSPIFQSEEANYYKSLKSNIKTSLISLSSASNTNLSVHSTQVQSPNTRIDFTSPDPYNNKCNSLKFPVQSRENQDFSDYIQLSQYLPQPRFYKTMYTSISSPKPFTENRDGKLMLNTFYKCNNSNSLGKPAENSRHEDPYQMIKKSNMEFSIQEETSNENVSSISDDFSSNMTSSPNNDLKNKQNPNMKFVTFRLDNAEITSGLSPSTSNESLTVITLEQSEAFLKELEGDAPDKIHFNKFRKEINHDFRDKFILPSAPTHCEADSFPLASHTKASLL 2217
            KSHIQWSAWVSVIFIGILQVYVVYSSLSRYIMLKDETFHGYHMHFAEYRWIFFLLNFQLFIIIGALGVLPIWFYSAMFSISSFFCKGFSPQLNRQGFKELVLKEAIEVFSQQKLEVNNSLHQDHPRNCSCQKTIRFGSFYPIASIFQVLIAYLLLLPLPFIESWLIQIDHKHKGGIQTNDAEFLVSNGLLLKLNWNVEYFNFLISLIGLSLQFPNFIWGANKLFSIAFSLLEFIIIFHILIDYPIISIVIKFLFKNCTACEDGTESVVVYSTILLISSVIGNLFLLLTFFSIFQFAHEKCVQSLLKSSWFFNNYRDDNVSFSTNLDQVPGQSNAMSSTLYSL+KIREVKTSLYPFTSAALFFVSGIALKCFTYYHFQEFRNTTKIFIIYFIQSAMLVFVYFLFWFGISLKPKWSFSIKDLENRTTIHSPIFQSEEANYYKSLKSNIKTSLISLSSASNTNLSVHSTQVQSPNTRIDFTSPDPYNNKCNSLKFPVQSRENQDFSDYIQL+QYLPQPRFYKTMYTSISSPKPFTENRDGKLMLNTFYKCNNSNSLGKPAENSRHEDPYQMIKKSNMEFSIQEETSNENVSSISDDFSSNMTSSPNNDLKNKQNPNMKFVTFRLDNAEITSGLSPSTSNESLTVITLEQSEAFLKELEGDAPDKIHFNKFRKEINHDFRDKFILPSAPTHCEADSFPLASHTKASLL
Sbjct:    4 KSHIQWSAWVSVIFIGILQVYVVYSSLSRYIMLKDETFHGYHMHFAEYRWIFFLLNFQLFIIIGALGVLPIWFYSAMFSISSFFCKGFSPQLNRQGFKELVLKEAIEVFSQQKLEVNNSLHQDHPRNCSCQKTIRFGSFYPIASIFQVLIAYLLLLPLPFIESWLIQIDHKHKGGIQTNDAEFLVSNGLLLKLNWNVEYFNFLISLIGLSLQFPNFIWGANKLFSIAFSLLEFIIIFHILIDYPIISIVIKFLFKNCTACEDGTESVVVYSTILLISSVIGNLFLLLTFFSIFQFAHEKCVQSLLKSSWFFNNYRDDNVSFSTNLDQVPGQSNAMSSTLYSLEKIREVKTSLYPFTSAALFFVSGIALKCFTYYHFQEFRNTTKIFIIYFIQSAMLVFVYFLFWFGISLKPKWSFSIKDLENRTTIHSPIFQSEEANYYKSLKSNIKTSLISLSSASNTNLSVHSTQVQSPNTRIDFTSPDPYNNKCNSLKFPVQSRENQDFSDYIQLNQYLPQPRFYKTMYTSISSPKPFTENRDGKLMLNTFYKCNNSNSLGKPAENSRHEDPYQMIKKSNMEFSIQEETSNENVSSISDDFSSNMTSSPNNDLKNKQNPNMKFVTFRLDNAEITSGLSPSTSNESLTVITLEQSEAFLKELEGDAPDKIHFNKFRKEINHDFRDKFILPSAPTHCEADSFPLASHTKASLL 707
[Close]
BLAST of SMED30023071 vs. Planmine SMEST
Match: SMESG000054475.1 (SMESG000054475.1)

HSP 1 Score: 1279.23 bits (3309), Expect = 0.000e+0
Identity = 671/673 (99.70%), Postives = 673/673 (100.00%), Query Frame = 1 
Query:  199 MLKDETFHGYHMHFAEYRWXXXXXXXXXXXXXGALGVLPIWFYSAMFSISSFFCKGFSPQLNRQGFKELVLKEAIEVFSQQKLEVNNSLHQDHPRNCSCQKTIRFGSFYPIASIFQVLIAYXXXXXXXFIESWLIQIDHKHKGGIQTNDAEFLVSNGLLLKLNWNVEYFNFLISLIGLSLQFPNFIWGANKLFSIAXXXXXXXXXXXXXXXXXXXSIVIKFLFKNCTACEDGTESVVVYSTILLISSVIGNLFLLLTFFSIFQFAHEKCVQSLLKSSWFFNNYRDDNVSFSTNLDQVPGQSNAMSSTLYSLKKIREVKTSLYPFTSAALFFVSGIALKCFTYYHFQEFRNTTKIFIIYFIQSAMLVFVYFLFWFGISLKPKWSFSIKDLENRTTIHSPIFQSEEANYYKSLKSNIKTSLISLSSASNTNLSVHSTQVQSPNTRIDFTSPDPYNNKCNSLKFPVQSRENQDFSDYIQLSQYLPQPRFYKTMYTSISSPKPFTENRDGKLMLNTFYKCNNSNSLGKPAENSRHEDPYQMIKKSNMEFSIQEETSNENVSSISDDFSSNMTSSPNNDLKNKQNPNMKFVTFRLDNAEITSGLSPSTSNESLTVITLEQSEAFLKELEGDAPDKIHFNKFRKEINHDFRDKFILPSAPTHCEADSFPLASHTKASLL 2217
            MLKDETFHGYHMHFAEYRWIFFLLNFQLFIIIGALGVLPIWFYSAMFSISSFFCKGFSPQLNRQGFKELVLKEAIEVFSQQKLEVNNSLHQDHPRNCSCQKTIRFGSFYPIASIFQVLIAYLLLLPLPFIESWLIQIDHKHKGGIQTNDAEFLVSNGLLLKLNWNVEYFNFLISLIGLSLQFPNFIWGANKLFSIAFSLLEFIIIFHILIDYPIISIVIKFLFKNCTACEDGTESVVVYSTILLISSVIGNLFLLLTFFSIFQFAHEKCVQSLLKSSWFFNNYRDDNVSFSTNLDQVPGQSNAMSSTLYSL+KIREVKTSLYPFTSAALFFVSGIALKCFTYYHFQEFRNTTKIFIIYFIQSAMLVFVYFLFWFGISLKPKWSFSIKDLENRTTIHSPIFQSEEANYYKSLKSNIKTSLISLSSASNTNLSVHSTQVQSPNTRIDFTSPDPYNNKCNSLKFPVQSRENQDFSDYIQL+QYLPQPRFYKTMYTSISSPKPFTENRDGKLMLNTFYKCNNSNSLGKPAENSRHEDPYQMIKKSNMEFSIQEETSNENVSSISDDFSSNMTSSPNNDLKNKQNPNMKFVTFRLDNAEITSGLSPSTSNESLTVITLEQSEAFLKELEGDAPDKIHFNKFRKEINHDFRDKFILPSAPTHCEADSFPLASHTKASLL
Sbjct:    1 MLKDETFHGYHMHFAEYRWIFFLLNFQLFIIIGALGVLPIWFYSAMFSISSFFCKGFSPQLNRQGFKELVLKEAIEVFSQQKLEVNNSLHQDHPRNCSCQKTIRFGSFYPIASIFQVLIAYLLLLPLPFIESWLIQIDHKHKGGIQTNDAEFLVSNGLLLKLNWNVEYFNFLISLIGLSLQFPNFIWGANKLFSIAFSLLEFIIIFHILIDYPIISIVIKFLFKNCTACEDGTESVVVYSTILLISSVIGNLFLLLTFFSIFQFAHEKCVQSLLKSSWFFNNYRDDNVSFSTNLDQVPGQSNAMSSTLYSLEKIREVKTSLYPFTSAALFFVSGIALKCFTYYHFQEFRNTTKIFIIYFIQSAMLVFVYFLFWFGISLKPKWSFSIKDLENRTTIHSPIFQSEEANYYKSLKSNIKTSLISLSSASNTNLSVHSTQVQSPNTRIDFTSPDPYNNKCNSLKFPVQSRENQDFSDYIQLNQYLPQPRFYKTMYTSISSPKPFTENRDGKLMLNTFYKCNNSNSLGKPAENSRHEDPYQMIKKSNMEFSIQEETSNENVSSISDDFSSNMTSSPNNDLKNKQNPNMKFVTFRLDNAEITSGLSPSTSNESLTVITLEQSEAFLKELEGDAPDKIHFNKFRKEINHDFRDKFILPSAPTHCEADSFPLASHTKASLL 673
[Close]
BLAST of SMED30023071 vs. Planmine SMEST
Match: SMESG000054475.1 (SMESG000054475.1)

HSP 1 Score: 1253.81 bits (3243), Expect = 0.000e+0
Identity = 660/662 (99.70%), Postives = 662/662 (100.00%), Query Frame = 1 
Query:  232 MHFAEYRWXXXXXXXXXXXXXGALGVLPIWFYSAMFSISSFFCKGFSPQLNRQGFKELVLKEAIEVFSQQKLEVNNSLHQDHPRNCSCQKTIRFGSFYPIASIFQVLIAYXXXXXXXFIESWLIQIDHKHKGGIQTNDAEFLVSNGLLLKLNWNVEYFNFLISLIGLSLQFPNFIWGANKLFSIAXXXXXXXXXXXXXXXXXXXSIVIKFLFKNCTACEDGTESVVVYSTILLISSVIGNLFLLLTFFSIFQFAHEKCVQSLLKSSWFFNNYRDDNVSFSTNLDQVPGQSNAMSSTLYSLKKIREVKTSLYPFTSAALFFVSGIALKCFTYYHFQEFRNTTKIFIIYFIQSAMLVFVYFLFWFGISLKPKWSFSIKDLENRTTIHSPIFQSEEANYYKSLKSNIKTSLISLSSASNTNLSVHSTQVQSPNTRIDFTSPDPYNNKCNSLKFPVQSRENQDFSDYIQLSQYLPQPRFYKTMYTSISSPKPFTENRDGKLMLNTFYKCNNSNSLGKPAENSRHEDPYQMIKKSNMEFSIQEETSNENVSSISDDFSSNMTSSPNNDLKNKQNPNMKFVTFRLDNAEITSGLSPSTSNESLTVITLEQSEAFLKELEGDAPDKIHFNKFRKEINHDFRDKFILPSAPTHCEADSFPLASHTKASLL 2217
            MHFAEYRWIFFLLNFQLFIIIGALGVLPIWFYSAMFSISSFFCKGFSPQLNRQGFKELVLKEAIEVFSQQKLEVNNSLHQDHPRNCSCQKTIRFGSFYPIASIFQVLIAYLLLLPLPFIESWLIQIDHKHKGGIQTNDAEFLVSNGLLLKLNWNVEYFNFLISLIGLSLQFPNFIWGANKLFSIAFSLLEFIIIFHILIDYPIISIVIKFLFKNCTACEDGTESVVVYSTILLISSVIGNLFLLLTFFSIFQFAHEKCVQSLLKSSWFFNNYRDDNVSFSTNLDQVPGQSNAMSSTLYSL+KIREVKTSLYPFTSAALFFVSGIALKCFTYYHFQEFRNTTKIFIIYFIQSAMLVFVYFLFWFGISLKPKWSFSIKDLENRTTIHSPIFQSEEANYYKSLKSNIKTSLISLSSASNTNLSVHSTQVQSPNTRIDFTSPDPYNNKCNSLKFPVQSRENQDFSDYIQL+QYLPQPRFYKTMYTSISSPKPFTENRDGKLMLNTFYKCNNSNSLGKPAENSRHEDPYQMIKKSNMEFSIQEETSNENVSSISDDFSSNMTSSPNNDLKNKQNPNMKFVTFRLDNAEITSGLSPSTSNESLTVITLEQSEAFLKELEGDAPDKIHFNKFRKEINHDFRDKFILPSAPTHCEADSFPLASHTKASLL
Sbjct:    1 MHFAEYRWIFFLLNFQLFIIIGALGVLPIWFYSAMFSISSFFCKGFSPQLNRQGFKELVLKEAIEVFSQQKLEVNNSLHQDHPRNCSCQKTIRFGSFYPIASIFQVLIAYLLLLPLPFIESWLIQIDHKHKGGIQTNDAEFLVSNGLLLKLNWNVEYFNFLISLIGLSLQFPNFIWGANKLFSIAFSLLEFIIIFHILIDYPIISIVIKFLFKNCTACEDGTESVVVYSTILLISSVIGNLFLLLTFFSIFQFAHEKCVQSLLKSSWFFNNYRDDNVSFSTNLDQVPGQSNAMSSTLYSLEKIREVKTSLYPFTSAALFFVSGIALKCFTYYHFQEFRNTTKIFIIYFIQSAMLVFVYFLFWFGISLKPKWSFSIKDLENRTTIHSPIFQSEEANYYKSLKSNIKTSLISLSSASNTNLSVHSTQVQSPNTRIDFTSPDPYNNKCNSLKFPVQSRENQDFSDYIQLNQYLPQPRFYKTMYTSISSPKPFTENRDGKLMLNTFYKCNNSNSLGKPAENSRHEDPYQMIKKSNMEFSIQEETSNENVSSISDDFSSNMTSSPNNDLKNKQNPNMKFVTFRLDNAEITSGLSPSTSNESLTVITLEQSEAFLKELEGDAPDKIHFNKFRKEINHDFRDKFILPSAPTHCEADSFPLASHTKASLL 662
[Close]
BLAST of SMED30023071 vs. Planmine SMEST
Match: SMESG000068495.1 (SMESG000068495.1)

HSP 1 Score: 68.9366 bits (167), Expect = 1.065e-11
Identity = 103/439 (23.46%), Postives = 193/439 (43.96%), Query Frame = 1 
Query:  121 WSAWVSVIFIGILQVYVVYSSLSRYIMLK----DETFHGYHMHFAEYRWXXXXXXXXXXXXXGALGVLPIWFYSAMFSISSFFCKGFSPQLNRQGFKELVLKEAIEVFSQQKLEVNNSLHQDHPRNCSCQKTIRFGSFYPIASIFQVLIAYXXXXXXXFIESWLIQIDHKHKGGIQTNDAEFLVSNGL-----LLKLNWNVE------YFNFLISLIGLSLQFPNFIWGANKLFSIAXXXXXXXXXXXXXXXXXXXSIVIKFLFKNCTACEDGTESV---VVYSTILLISSVIGNLFLLLTFFSIFQFAHEKCVQSLLKSSWFFNNYRDDNVSFSTNLDQVPGQSNAMSSTLYS----LKKIREVKTSLYPFTS---AALFFVSGIALKCFTYYHFQEFRNTTKIFIIYF--IQSAMLVFVYFLFWFGISLKPKWSFSI 1356
            W  W+  IF+ IL +Y++Y+S+  Y+ LK    D  + G         W    +NFQL  ++ ++ +LPI   S+M    +    G    L +       + ++ E     ++++  S    +  +C  Q +I+   F PI+ + Q++  +  LLPLPFIE+  IQ        +   D +FL+   +       +  WN +      YFN +  L  L+ ++P+  W  N+ FS+ F L+  ++  HIL+DY   SI+IK          D        ++   I+ + S++G++ LLL   ++F +   +   +L++            +  +  +    G SN  S TL +       + E       + S   A +F ++G+++K   +       + T   ++Y   I + +    + + WFG+SL  KW F +
Sbjct:   12 WRIWI-FIFLAILHIYLIYTSIVDYLRLKALSSDPIYGG--------NWKPGPMNFQLSCLLCSITLLPIILLSSMIVTDNLANDGHV--LGKDIDNNHFIFDSDERGQLIRIDMKKSQSDKNLFSCGVQASIK-QQFLPISIVLQIISGFCYLLPLPFIEAQKIQHQINKPNHLWHTDLDFLMDPDIDHEDFTNQNQWNFQLPMRPAYFNMIFCLSQLTFRYPSVFWRTNRKFSLLFYLILILLSLHILVDYSSTSILIKLALS-----PDFISPALLPLLSPIIICVISIVGSMILLLMQITLFNYGLNQYFSNLIRHRQTLR--LQFKIEHNKKISDQTGSSNVSSETLNANDTNSSDLSECDFQFNKYLSHFFAIIFGLAGMSIKIPLFLQNSNICSLTGDRVMYLNIIITTIFSTCWLITWFGLSLHNKWQFKV 431
The following BLAST results are available for this feature:
BLAST of SMED30023071 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30023071 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30023071 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30023071 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30023071 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30023071 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30023071 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30023071 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30023071 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30023071 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30023071 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30023071 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30023071 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30023071 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000054475.10.000e+099.72SMESG000054475.1[more]
SMESG000054475.10.000e+099.72SMESG000054475.1[more]
SMESG000054475.10.000e+099.70SMESG000054475.1[more]
SMESG000054475.10.000e+099.70SMESG000054475.1[more]
SMESG000068495.11.065e-1123.46SMESG000068495.1[more]
back to top
Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30023071 ID=SMED30023071|Name=SMED30023071|organism=Schmidtea mediterranea sexual|type=transcript|length=2401bp
CAAATGTGCGTATGAGATAATAATTTCAGTGAATTCCAATAGTTTCCTGT
TCAAACTTGATTCGTAGTACTGCAATTGTCAACATCATTTATGATTTGCC
ATTTTAAATCACATATTCAGTGGTCTGCCTGGGTTTCGGTAATATTCATA
GGTATTTTGCAAGTTTATGTAGTGTATTCTTCACTCTCTCGGTATATAAT
GCTGAAAGATGAAACATTTCATGGTTATCATATGCATTTTGCTGAATATA
GATGGATTTTCTTTCTTTTGAATTTTCAATTATTTATCATAATTGGGGCT
TTAGGAGTTTTGCCGATTTGGTTTTATAGTGCCATGTTTTCAATTTCATC
GTTTTTCTGTAAAGGATTTTCTCCCCAATTAAATCGCCAGGGATTTAAAG
AACTAGTTTTAAAAGAAGCCATAGAAGTTTTTAGTCAACAAAAGCTAGAA
GTCAACAATTCTTTGCATCAAGATCATCCAAGAAACTGCAGCTGTCAAAA
AACCATAAGATTTGGCTCATTCTATCCTATTGCTAGCATATTTCAGGTTT
TAATCGCGTATTTATTGTTATTACCTCTTCCATTTATTGAAAGTTGGCTT
ATACAAATTGATCACAAACATAAAGGCGGAATACAAACAAATGATGCTGA
ATTTTTGGTTTCAAATGGTTTATTATTAAAACTTAATTGGAATGTTGAAT
ATTTCAATTTTTTGATAAGTTTAATTGGACTTTCTTTACAATTTCCTAAT
TTCATTTGGGGGGCCAATAAATTGTTTTCCATTGCATTCAGTCTTTTAGA
ATTTATTATTATTTTTCACATTTTGATTGATTATCCAATTATTTCCATTG
TGATAAAATTCTTATTCAAAAATTGTACGGCTTGTGAAGATGGCACTGAA
AGTGTTGTGGTTTATTCAACAATCCTTTTGATATCCTCTGTAATTGGCAA
TCTGTTCCTGCTTCTTACATTTTTTTCAATTTTCCAATTTGCCCATGAAA
AATGTGTCCAGTCACTTTTGAAAAGTTCGTGGTTTTTCAATAATTATCGC
GACGACAATGTATCATTCTCGACGAACCTTGATCAAGTTCCTGGCCAAAG
TAATGCGATGAGTTCAACGCTATATTCTTTGAAAAAAATCCGAGAAGTCA
AGACAAGCTTATATCCGTTTACGTCTGCCGCTTTGTTTTTCGTTTCTGGG
ATCGCTTTGAAATGTTTTACGTATTATCATTTTCAGGAATTCAGAAACAC
TACCAAGATATTCATCATTTATTTTATTCAATCAGCCATGCTTGTATTTG
TGTACTTTTTGTTTTGGTTTGGTATATCGCTAAAGCCAAAGTGGTCCTTT
TCAATTAAAGATCTTGAAAATAGAACAACCATACATTCACCAATATTCCA
AAGTGAAGAAGCTAATTATTACAAGTCTCTTAAAAGTAACATAAAGACTA
GTCTAATATCATTATCATCGGCTTCGAATACAAATCTGAGTGTCCATTCC
ACTCAAGTGCAGTCTCCTAACACTCGGATTGATTTTACCAGTCCAGACCC
CTACAATAATAAATGCAACAGCTTAAAATTTCCAGTTCAAAGTAGAGAAA
ATCAAGATTTCTCTGACTATATTCAACTGAGTCAATATTTACCCCAACCA
CGTTTTTATAAAACAATGTACACCAGTATATCTTCACCAAAACCTTTTAC
TGAAAATCGCGATGGAAAGTTAATGTTGAACACGTTTTATAAATGTAATA
ATTCAAATTCACTGGGGAAACCGGCGGAAAATTCCCGCCACGAAGATCCT
TATCAAATGATCAAAAAATCAAACATGGAATTTTCAATTCAAGAAGAAAC
TTCAAACGAAAATGTTTCTTCAATAAGCGATGATTTCTCCAGCAATATGA
CGTCTTCTCCAAATAATGACTTGAAAAATAAACAAAATCCTAACATGAAA
TTTGTCACTTTTCGTTTAGACAACGCAGAAATAACTTCAGGTTTGTCACC
AAGTACCAGCAATGAAAGTCTAACAGTAATTACTCTTGAACAGTCTGAAG
CTTTTCTCAAGGAACTGGAAGGAGACGCTCCAGACAAAATACATTTCAAT
AAATTTAGAAAAGAAATTAATCATGATTTCCGAGATAAATTTATACTGCC
TTCGGCTCCCACACATTGTGAGGCAGATTCTTTTCCTCTGGCATCACACA
CAAAAGCATCTCTTTTATGAACTGAAATTTGTTAAATCTAAATGCTAACA
GAATTTTAGACTATTCATTCTATGTTATGCTTAAATCATATTTGCGTTGC
GATAGTGACTCTCATTTCTCATTGACCGCTTGACGTGCTCACCCCTCATT
GTGTTCATCGTCTTCTATATTACACAAACAAATATACGCGTTTATTAACC
G
back to top

protein sequence of SMED30023071-orf-1

>SMED30023071-orf-1 ID=SMED30023071-orf-1|Name=SMED30023071-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=710bp
MICHFKSHIQWSAWVSVIFIGILQVYVVYSSLSRYIMLKDETFHGYHMHF
AEYRWIFFLLNFQLFIIIGALGVLPIWFYSAMFSISSFFCKGFSPQLNRQ
GFKELVLKEAIEVFSQQKLEVNNSLHQDHPRNCSCQKTIRFGSFYPIASI
FQVLIAYLLLLPLPFIESWLIQIDHKHKGGIQTNDAEFLVSNGLLLKLNW
NVEYFNFLISLIGLSLQFPNFIWGANKLFSIAFSLLEFIIIFHILIDYPI
ISIVIKFLFKNCTACEDGTESVVVYSTILLISSVIGNLFLLLTFFSIFQF
AHEKCVQSLLKSSWFFNNYRDDNVSFSTNLDQVPGQSNAMSSTLYSLKKI
REVKTSLYPFTSAALFFVSGIALKCFTYYHFQEFRNTTKIFIIYFIQSAM
LVFVYFLFWFGISLKPKWSFSIKDLENRTTIHSPIFQSEEANYYKSLKSN
IKTSLISLSSASNTNLSVHSTQVQSPNTRIDFTSPDPYNNKCNSLKFPVQ
SRENQDFSDYIQLSQYLPQPRFYKTMYTSISSPKPFTENRDGKLMLNTFY
KCNNSNSLGKPAENSRHEDPYQMIKKSNMEFSIQEETSNENVSSISDDFS
SNMTSSPNNDLKNKQNPNMKFVTFRLDNAEITSGLSPSTSNESLTVITLE
QSEAFLKELEGDAPDKIHFNKFRKEINHDFRDKFILPSAPTHCEADSFPL
ASHTKASLL*
back to top
Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0002032epidermal cell
PLANA:0002109X1 cell
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 584..617
NoneNo IPR availablePANTHERPTHR21579FAMILY NOT NAMEDcoord: 11..626
NoneNo IPR availableTMHMMTMhelixcoord: 272..294
NoneNo IPR availableTMHMMTMhelixcoord: 57..79
NoneNo IPR availableTMHMMTMhelixcoord: 238..259
NoneNo IPR availableTMHMMTMhelixcoord: 355..377
NoneNo IPR availableTMHMMTMhelixcoord: 204..223
NoneNo IPR availableTMHMMTMhelixcoord: 390..412
NoneNo IPR availableTMHMMTMhelixcoord: 10..32
NoneNo IPR availableTMHMMTMhelixcoord: 149..171