EF-hand calcium-binding domain-containing protein 6
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30023059 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 13Note: Hover over icons to view figure legend
Homology
BLAST of EF-hand calcium-binding domain-containing protein 6 vs. Ensembl Human
Match: EFCAB6 (EF-hand calcium binding domain 6 [Source:HGNC Symbol;Acc:HGNC:24204]) HSP 1 Score: 219.935 bits (559), Expect = 4.816e-57 Identity = 256/1150 (22.26%), Postives = 479/1150 (41.65%), Query Frame = 3
Query: 687 QISRNPNGIMKAFKLFDFNSDYCIQRHRLKKVMENFCCPLTEQQFDKLVARFSQSQNGNVNYMEFMKGMGIPIDNQGTIQTKNSEETI-NIDTRNTKGSRIFEAALIENGLLGSNELEQLLVIFRKKIEDQFSVLRKTFLVMDPNAEGFVTLEILYKILNQFAFPMSLNVFNQIMDKCAFKATGKIIWLSFLQKFQDPQSKHLNGSTIAIKSDHKCNPIQGVNSKPNIDDLLELLSNHISNNYSSFRDAFLQINQTHKGKIPKSEFLKILNRFQFYISADQLSIILKFLDPHNEGYISYHHFLQLFEQKENLSAHKWLNSNHKVVEPRKDLQLSSETIKSTISDNIGNKWSNVVHEFNLVDLNRTGYISVEDLKKIFKKLFGYLKDDDIDRLVAPLTLKD-KKGRINYLELFNLLNVDTSPSDLEGLSTQIAESNDAQLKERHENHIEKMESIEKLGEARLNLMS----------PEELIVRIKDRIVQNRLNYHELYKKYECNDKKGGINKSQLRKMLTQFGYTLTNEQFSKISEILNFPTSSKSRIDFN-GFLAIFADNRESSNDLMTDVCFLPKSNHHVNDIRGDDNRNMNCQEVEDLLRQKIVNNFPDLMIAFHKFDRSNKGYLVKSDFQNFLLGNLIMIENDQFDLLCEKLGVGEKSKLTYNDFLNRFQIRDDMVDGHKWLDSKHRFNDPSTN-AVPDMSAEDVYMLLPEKIYQYFHEISPTFIALVTSGTGFIRFRNLQHLLSRLQISMSKSEMKKLWKRYDTNNRNKLDYHDFLYNLGLLEKFGCGDLMGPSAAIIQNSHNALVDHLVN---QETKHQQSVDNQAVL--GTHLHVEHLFEMVKRHLRVAYSDSALAFKAVANKKTLSGFFTINDLNKLLSQLHMFVTNDKFVQLAARMGFEGPDDSLSYEKFCCIFQDSLNIDGQEK-TVGSIRINQDLENPSKIGIEEAGNLNSTQIASIIGKRIVDQYSEIEKTFLVMDPENSGYVSMNEFRNLLVGFCLSLTDKQFNKYRNSLSTYIVKSANTDDKIDYRYFMKGFN----QKLNNLDIQXXXXXXXXXXXXXTYQQIQEHINESIKVRPKLFLNSFKSKDYASLNFISKSDFREIIERFLFTVPDSHFEKFWSNLKKNEFDNVNYGEFV 4064
++ +N +MKAF+L D N ++ L++V+E FC L +++++K ++ ++ V+Y F+K + I D N E ++ N +N+K R+ +A E+ + + L+++ F ++ + + K DP G+V+ L +L+ F + + +F Q+M + KAT KI W FL F +PQ ++ K N I N + ++++ L H ++ +S + A L IN G I + EF ILN +S + +++ LDP + G ++ F+ L E EN K + + L+ ++++ + D I + DL TG I + KKI +L + +L + ++D GRI Y +L + +D P+ + + D L E H++K E + R L EE+I + K I Q + + + + + G IN +K+L G + ++Q++ ++ + F S +DF GF ++ T S +VN + + +E L +++ +F D AF K D G + D LL L+ +++D+F+ LG+ L + +F N + R D ++ R P A +++ E + L K + ++S F+ G G +R R++++ L I ++ E +KLW RYDT + + Y +FL LG+ S A+ H + N TK QQ + L T V +++ RH D + AF KT + +I + ++L + +T + L G D++++Y F ++S + Q K S+ IN NP + + + S+Q+A + F +D E++G+V EF +L FC LTD Q++ + L ++ I+++YF++ F+ + + + P + + I +++++ F++ D N IS+ +FR I R + + D F++ W+ + N + Y +F+
Sbjct: 17 KVFKNIKTVMKAFELIDVNKTGLVRPQELRRVLETFCMKLRDEEYEKFSKHYNIHKDTAVDYNVFLKNLSINNDLNLRYCMGNQEVSLENQQAKNSKKERLLGSASSED-IWRNYSLDEIERNFCLQLSKSYEKVEKALSAGDPCKGGYVSFNYLKIVLDTFVYQIPRRIFIQLMKRFGLKATTKINWKQFLTSFHEPQGLQVSSKGPLTKR----NSINSRNES-HKENIITKLFRHTEDHSASLKKALLIINTKPDGPITREEFRYILNCMAVKLSDSEFKELMQMLDPGDTGVVNTSMFIDLIE--ENCRMRK----TSPCTDAKTPFLLAWDSVEEIVHDTITRNLQAFYNMLRSYDLGDTGRIGRNNFKKIMHVFCPFLTNAHFIKLCS--KIQDIGSGRILYKKLLACIGIDGPPT-----VSPVLVPKDQLLSE----HLQKDEQQQPDLSERTKLTEDKTTLTKKMTTEEVIEKFKKCIQQQDPAFKKRFLDFS-KEPNGKINVHDFKKVLEDTGMPMDDDQYALLTTKIGFEKEGMSYLDFAAGF-----EDPPMRGPETTPPQPPTPSKSYVN------SHFITAEECLKLFPRRLKESFRDPYSAFFKTDADRDGIINMHDLHRLLLHLLLNLKDDEFERFLGLLGLRLSVTLNFREFQNLCEKRPWRTD-----EAPQRLIRPKQKVADSELACEQAHQYLVTKAKNRWSDLSKNFLETDNEGNGILRRRDIKNALYGFDIPLTPREFEKLWARYDTEGKGHITYQEFLQKLGI----------NYSPAV----HRPCAEDYFNFMGHFTKPQQLQEEMKELQQSTEKAVAARDKLMDRH-----QDISKAFTKTDQSKT--NYISICKMQEVLEECGCSLTEGELTHLLNSWGVSRHDNAINYLDFLRAVENSKSTGAQPKEKEESMPINFATLNPQEAVRKIQEVVESSQLA-------------LSTAFSALDKEDTGFVKATEFGQVLKDFCYKLTDNQYHYFLRKLRIHLTPY------INWKYFLQNFSCFLEETADEWAEKMPKGPPPTSPKATADRDILARLHKAVTSHYHAITQEFENFDTMKTNTISREEFRAICNRRVQILTDEQFDRLWNEMPVNAKGRLKYPDFL 1086 HSP 2 Score: 84.3445 bits (207), Expect = 2.005e-15 Identity = 126/678 (18.58%), Postives = 265/678 (39.09%), Query Frame = 3
Query: 1380 ELLSNHISNNYSSFRDAFLQINQTHKGKIPKSEFLKILNRFQFYISADQLSIILKFLDPHNEGYISYHHFLQLFEQKENLSAHKWLNSNH-----------KVVEPRKDLQLSSETIKSTISDNIGNKWSNVVHEFNLVDLNRTGYISVEDLKKIFKKLFGYLKDDDIDRLVAPLTLKDKKGRINYLELFNLLNVDTSPSDLEGLSTQIAESNDAQLKERHENHIEKMESIEKLGEARLNLMSPEELIVRIKDRIVQNRLNYHELYKKYECNDKKGGINKSQLRKMLTQFGYTLTNEQFSKISEILNFPTSSKSRIDFNGFLAIFADNRESSNDLMTDVCFLPKSNHHVNDIRGDDNRNMNCQEVEDLLRQKIVNNFPDLMIAFHKFDRSNKGYLVKSDFQNFLLGNLIMIENDQFDLLCEKLGVGEKSKLTYNDFLNRFQIR--------------------DDMVDGHKWLDS-------------KHRFNDPSTNAVPDMS----AEDVYMLLPEKIYQYFHEISPTFIALVTSGTGFIRFRNLQHLLSRLQISMSKSEMKKLWKRYDTNNRNKLDYHDFLYNLGLLEKFGCGDLMGPSAAIIQNSHNALVDHLVNQETKHQQSVDNQAVLGTHLHVEHLFEMVKRHLRVAYSDSALAFKAVANKKTLSGFFTIN 3269
+ L N +S FL+ + G + + + L F ++ + + D +G+I+Y FLQ + + H+ ++ ++ E K+LQ S+E + D + ++ ++ F D ++T YIS+ ++++ ++ L + ++ L+ + INYL+ + + ++S AQ KE+ E+ + A LN P+E + +I++ + ++L + + D G + ++ ++L F Y LT+ Q+ L + I++ FL F+ E + D + +PK + D +++ L + + +++ + F FD + + +F+ + ++ ++QFD L ++ V K +L Y DFL+RF D+ +G + S H ST +P + + L ++I + ++ + G I + L+ + + +SK E ++L +YD + K Y DF+ + LL K LM IQN+H +E + A+L + H + ++R + +Y ++ +VA+ +T+ ++IN
Sbjct: 687 QYLVTKAKNRWSDLSKNFLETDNEGNGILRRRDIKNALYGFDIPLTPREFEKLWARYDTEGKGHITYQEFLQKLGINYSPAVHRPCAEDYFNFMGHFTKPQQLQEEMKELQQSTEKAVAA-RDKLMDRHQDISKAFTKTDQSKTNYISICKMQEVLEECGCSLTEGELTHLLNSWGVSRHDNAINYLDFLR--------------AVENSKSTGAQPKEKEESM--------PINFATLN---PQEAVRKIQEVVESSQLALSTAFSALDKEDT-GFVKATEFGQVLKDFCYKLTDNQYHYFLRKLRIHLTP--YINWKYFLQNFSCFLEETADEWAEK--MPKGPPPTSPKATAD------RDILARLHKAVTSHYHAITQEFENFDTMKTNTISREEFRAICNRRVQILTDEQFDRLWNEMPVNAKGRLKYPDFLSRFSSETAATPMATGDSAVAQRGSSVPDVSEGTRSALSLPTQELRPGSKSQSHPCTPASTTVIPGTPPLQNCDPIESRLRKRIQGCWRQLLKECKEKDVARQGDINASDFLALVEKFNLDISKEECQQLIIKYDLKSNGKFAYCDFIQSCVLLLKAKESSLM--HRMKIQNAHKM-------KEAGAETPSFYSALLRIQPKIVHCWRPMRRTFK-SYDEAGTGLLSVADFRTVLRQYSIN 1317 HSP 3 Score: 76.2554 bits (186), Expect = 4.506e-13 Identity = 99/475 (20.84%), Postives = 196/475 (41.26%), Query Frame = 3
Query: 195 ISEINPIVKSQLLKNHKEILKALQTFDIECSGLVTKGELRRVLDSYCLPMTTDQYEQFLSHNSINYSKGSINYKELIDKIYDKTSAVGH--FKKNIKLPQLQRSATTICLQNKHFTLPTTRRSSGKISRSQLFXXXXXXXXXXXXXXXXWNYNAYTIDDIIPILQMQISRNPNGIMKAFKLFDFNSDYCIQRHRLKKVMENFCCPLTEQQFDKLVARFSQSQNGNVNYMEFMKGM----GIPIDNQGTIQTKNSEETINIDTRNTKGSRIFEAALIENGLLGSNELEQLLVIFRKKIEDQFSVLRKTFLVMDPNAEGFVTLEILYKILNQFAFPMSLNVFNQIMDKCAFKATGKIIWLSFLQKFQDPQSKHLNGSTIAIKSDHKCNPIQGVNSKPNI---DDLLELLSNHISNNYSSFRDAFLQINQTHKGKIPKSEFLKILNRFQFYISADQLSIILKFLDPHNEGYISYHHFL 1592
+ E+ V ++ KN K ++KA + D+ +GLV ELRRVL+++C+ + ++YE+F H +I+ K +AV + F KN+ + C+ N+ +L + + K R L S S W Y++D+I +Q+S++ + KA D + + LK V++ F + + F +L+ RF +N+ +F+ G+ + ++G + +NS I++RN + E ++ + ED + L+K L+++ +G +T E ILN A +S + F ++M TG + F+ ++ ++ C + P + D + E++ + I+ N +F + + G+I ++ F KI++ F +++ + + G I Y L
Sbjct: 7 LRELEIQVGEKVFKNIKTVMKAFELIDVNKTGLVRPQELRRVLETFCMKLRDEEYEKFSKHYNIH-----------------KDTAVDYNVFLKNLSIN--NDLNLRYCMGNQEVSLENQQAKNSKKER----------LLGSASSEDIW--RNYSLDEIERNFCLQLSKSYEKVEKALSAGDPCKGGYVSFNYLKIVLDTFVYQIPRRIFIQLMKRFGLKATTKINWKQFLTSFHEPQGLQVSSKGPLTKRNS-----INSRN------------------ESHKENIITKLFRHTEDHSASLKKALLIINTKPDGPITREEFRYILNCMAVKLSDSEFKELMQMLDPGDTGVVNTSMFIDLIEE---------NCRMRKTSPCTDAK----TPFLLAWDSVEEIVHDTITRNLQAFYNMLRSYDLGDTGRIGRNNFKKIMHVFCPFLTNAHFIKLCSKIQDIGSGRILYKKLL 414 HSP 4 Score: 68.1662 bits (165), Expect = 1.516e-10 Identity = 49/212 (23.11%), Postives = 97/212 (45.75%), Query Frame = 3
Query: 4161 TDEMDTDESIRKNLERLLNRNWQSIFNKCKAIDQAINECQTAEGSIPIDDFVYILSEYDDSLSAENLAAINL-YKTCKNGRFIKYGDLIRSIVL---SSRGSLISNENVTEVQKKFLNVGIKTTNSDNSKDYFPQLFKLRRQILENWKDLRKNFLQSDKKQNGAVSGNIFMTIMKTVCGQLSADLFYHLMKIYDQDGTGNFHYKRFIEDFLK 4784
T + + I L + + W+ + +CK D A +G I DF+ ++ +++ +S E + + Y NG+F Y D I+S VL + SL+ + K G +T + ++ L +++ +I+ W+ +R+ F D+ G +S F T+++ LS + F+H+++ YD+ + Y F+ FL+
Sbjct: 1152 TPPLQNCDPIESRLRKRIQGCWRQLLKECKEKDVA------RQGDINASDFLALVEKFNLDISKEECQQLIIKYDLKSNGKFA-YCDFIQSCVLLLKAKESSLMHRMKIQNAHK-MKEAGAETPS------FYSALLRIQPKIVHCWRPMRRTFKSYDEAGTGLLSVADFRTVLRQYSINLSEEEFFHILEYYDKTLSSKISYNDFLRAFLQ 1349 HSP 5 Score: 58.5362 bits (140), Expect = 1.255e-7 Identity = 129/719 (17.94%), Postives = 267/719 (37.13%), Query Frame = 3
Query: 1002 IENGLLGSNELEQLLVIFRKKIEDQFSVLRKTFLVMDPNAEGFVTLEILYKILNQFAFPMSLNVFNQIMDKCAFKATGKIIWLSFLQKFQ---DPQSKHLNGSTIAIKSDHKCNPIQGVNSKPNIDDLLE---LLSNHISNNYSSFRDAFLQINQTHKGKIPKSEFLKILNRFQFYISADQLSIIL-KFLDPHNEGYISYHHFLQLFEQKENLSAH-----KWLNSNHKVVEPRKDLQLSSETIKSTISDNIGNKWSNVVHEFNLVDLNRTGYISVEDLKKIFKKLFGYLKDDDIDRLVAPLTLKDKKGRINYLELFNLLNVDTSPSDLEGLSTQIAESNDAQLKERHENHIEKMESIEKLGEARLNLMSPEELIVRIKDRIVQNRLN------YHELYKKYECND--KKGGINKSQLRKMLTQFGYTLTNEQFSKISEILNFPTSSKSRIDFNGFLAIFADNRESSNDLMTDVCFLPKSNHHVNDIR----------------GDDNRN-----------------MNCQEVEDLLRQKIVNNFPDLMIAFHKFDRSNKGYLVKSDFQNFLLGNLIMIENDQFDLLCEKLGVGEKSKLTYNDFLNR----FQIRDDMVDGHKWLDSKHRFNDPSTNAVPDMSAEDVYMLLPEKIYQYFHEISPTFIALVTSGTGFIRFRNLQHLLSRLQISMSKSEMKKLWKRYDTNNRNKLDYHDFL 2987
+ + L + Q LV K ++++S L K FL D G + + L F P++ F ++ + + G I + FLQK P H P Q + E + + + + AF + +Q+ I + ++L ++ +L+ +L + ++ I+Y FL+ E ++ A + + N + P++ ++ E ++S+ + F+ +D TG++ + ++ K L D+ + +K RI+ N L+ S + E+ D E EK+ + P DR + RL+ YH + +++E D K I++ + R + + LT+EQF ++ P ++K R+ + FL+ F+ + ++ + T + + V D+ G +++ NC +E LR++I + L+ + D + +G + SDF + + I ++ L K + K Y DF+ + ++ + + + H+ + SA + + KI + + TF + +GTG + + + +L + I++S+ E + + YD +K+ Y+DFL
Sbjct: 675 VADSELACEQAHQYLVT---KAKNRWSDLSKNFLETDNEGNGILRRRDIKNALYGFDIPLTPREFEKLWARYDTEGKGHITYQEFLQKLGINYSPAVHRPCAEDYFNFMGHFTKPQQLQEEMKELQQSTEKAVAARDKLMDRHQDISKAFTKTDQSKTNYISICKMQEVLEECGCSLTEGELTHLLNSWGVSRHDNAINYLDFLRAVENSKSTGAQPKEKEESMPINFATLNPQEAVRKIQEVVESS--------QLALSTAFSALDKEDTGFVKATEFGQVLKDFCYKLTDNQYHYFL-------RKLRIHLTPYINWKYF------LQNFSCFLEETAD--------------EWAEKMPKG-----PPPTSPKATADRDILARLHKAVTSHYHAITQEFENFDTMKTNTISREEFRAICNRRVQILTDEQFDRLWN--EMPVNAKGRLKYPDFLSRFS-SETAATPMATGDSAVAQRGSSVPDVSEGTRSALSLPTQELRPGSKSQSHPCTPASTTVIPGTPPLQNCDPIESRLRKRIQGCWRQLLKECKEKDVARQGDINASDFLALVEKFNLDISKEECQQLIIKYDLKSNGKFAYCDFIQSCVLLLKAKESSLMHRMKIQNAHKMKEAGAETPSFYSA---LLRIQPKIVHCWRPMRRTFKSYDEAGTGLLSVADFRTVLRQYSINLSEEEFFHILEYYDKTLSSKISYNDFL 1344 HSP 6 Score: 56.9954 bits (136), Expect = 4.021e-7 Identity = 78/428 (18.22%), Postives = 176/428 (41.12%), Query Frame = 3
Query: 2787 EKIYQYFHEISPTFIALVTSGTGFIRFRNLQHLLSRLQISMSKSEMKKLWKRYDTNNRNKLDYHDFLYNLGLLEKFGCGDLMGPSAAIIQNSHNALVDHLVNQETKHQQSVDNQAVLGTH----LHVEHLFEMVKRH--LRVAYSDSALAFKAVANKKTLSGFFTINDLNKLLSQLHMFVTNDKFVQLAARMGFEGPDDSLSYEKFCCIFQDSLNIDGQEKTVGSIRINQDLENPSKIGIEEAGNLNS------TQIASIIGKRIVDQYSEIEKTFLVMDPENSGYVSMNEFRNLLVGFCLSLTDKQFNKYRNSL---STYIVKSANTDDKIDYRYFMKGFNQKLNNLDIQXXXXXXXXXXXXXTYQQIQEHINESIKVRPKLFLNSFKSKDYASLNFISKSDFREIIERFLFTVPDSHFEKFWSNLK 4025
EK+++ + F + + TG +R + L+ +L + + E +K K Y+ + +DY+ FL NL + MG + V+ E + ++ + +LG+ + + + ++R+ L+++ S + A G+ + N L +L + F+QL R G + +++++F F + Q L+ SK + + ++NS I + + + D + ++K L+++ + G ++ EFR +L + L+D +F + L T +V ++ D I+ M+ ++ A + + ++E ++++I + F N +S D I +++F++I+ F + ++HF K S ++
Sbjct: 16 EKVFKNIKTVMKAFELIDVNKTGLVRPQELRRVLETFCMKLRDEEYEKFSKHYNIHKDTAVDYNVFLKNLSINNDLNLRYCMG--------------NQEVSLENQQAKNSKKERLLGSASSEDIWRNYSLDEIERNFCLQLSKSYEKVEKALSAGDPCKGGYVSFNYLKIVLDTFVYQIPRRIFIQLMKRFGLKA-TTKINWKQFLTSFHEP----------------QGLQVSSKGPLTKRNSINSRNESHKENIITKLFRHTEDHSASLKKALLIINTKPDGPITREEFRYILNCMAVKLSDSEFKELMQMLDPGDTGVVNTSMFIDLIEENCRMR-----------KTSPCTDAKTPFLLAWDSVEEIVHDTITRNLQAFYNMLRSYDLGDTGRIGRNNFKKIMHVFCPFLTNAHFIKLCSKIQ 401
BLAST of EF-hand calcium-binding domain-containing protein 6 vs. Ensembl Human
Match: EFCAB6 (EF-hand calcium binding domain 6 [Source:HGNC Symbol;Acc:HGNC:24204]) HSP 1 Score: 219.164 bits (557), Expect = 9.005e-57 Identity = 255/1152 (22.14%), Postives = 481/1152 (41.75%), Query Frame = 3
Query: 687 QISRNPNGIMKAFKLFDFNSDYCIQRHRLKKVMENFCCPLTEQQFDKLVARFSQSQNGNVNYMEFMKGMGIPIDNQGTIQTKNSEETI-NIDTRNTKGSRIFEAALIENGLLGSNELEQLLVIFRKKIEDQFSVLRKTFLVMDPNAEGFVTLEILYKILNQFAFPMSLNVFNQIMDKCAFKATGKIIWLSFLQKFQDPQSKHLNGSTIAIKSDHKCNPIQGVNSK--PNIDDLLELLSNHISNNYSSFRDAFLQINQTHKGKIPKSEFLKILNRFQFYISADQLSIILKFLDPHNEGYISYHHFLQLFEQKENLSAHKWLNSNHKVVEPRKDLQLSSETIKSTISDNIGNKWSNVVHEFNLVDLNRTGYISVEDLKKIFKKLFGYLKDDDIDRLVAPLTLKD-KKGRINYLELFNLLNVDTSPSDLEGLSTQIAESNDAQLKERHENHIEKMESIEKLGEARLNLMS----------PEELIVRIKDRIVQNRLNYHELYKKYECNDKKGGINKSQLRKMLTQFGYTLTNEQFSKISEILNFPTSSKSRIDFN-GFLAIFADNRESSNDLMTDVCFLPKSNHHVNDIRGDDNRNMNCQEVEDLLRQKIVNNFPDLMIAFHKFDRSNKGYLVKSDFQNFLLGNLIMIENDQFDLLCEKLGVGEKSKLTYNDFLNRFQIRDDMVDGHKWLDSKHRFNDPSTN-AVPDMSAEDVYMLLPEKIYQYFHEISPTFIALVTSGTGFIRFRNLQHLLSRLQISMSKSEMKKLWKRYDTNNRNKLDYHDFLYNLGLLEKFGCGDLMGPSAAIIQNSHNALVDHLVN---QETKHQQSVDNQAVL--GTHLHVEHLFEMVKRHLRVAYSDSALAFKAVANKKTLSGFFTINDLNKLLSQLHMFVTNDKFVQLAARMGFEGPDDSLSYEKFCCIFQDSLNIDGQEK-TVGSIRINQDLENPSKIGIEEAGNLNSTQIASIIGKRIVDQYSEIEKTFLVMDPENSGYVSMNEFRNLLVGFCLSLTDKQFNKYRNSLSTYIVKSANTDDKIDYRYFMKGFN----QKLNNLDIQXXXXXXXXXXXXXTYQQIQEHINESIKVRPKLFLNSFKSKDYASLNFISKSDFREIIERFLFTVPDSHFEKFWSNLKKNEFDNVNYGEFV 4064
++ +N +MKAF+L D N ++ L++V+E FC L +++++K ++ ++ V+Y F+K + I D N E ++ N +N+K R+ +A E+ + + L+++ F ++ + + K DP G+V+ L +L+ F + + +F Q+M + KAT KI W FL F +PQ ++ K + +NS+ + ++++ L H ++ +S + A L IN G I + EF ILN +S + +++ LDP + G ++ F+ L E EN K + + L+ ++++ + D I + DL TG I + KKI +L + +L + ++D GRI Y +L + +D P+ + + D L E H++K E + R L EE+I + K I Q + + + + + G IN +K+L G + ++Q++ ++ + F S +DF GF ++ T S +VN + + +E L +++ +F D AF K D G + D LL L+ +++D+F+ LG+ L + +F N + R D ++ R P A +++ E + L K + ++S F+ G G +R R++++ L I ++ E +KLW RYDT + + Y +FL LG+ S A+ H + N TK QQ + L T V +++ RH D + AF KT + +I + ++L + +T + L G D++++Y F ++S + Q K S+ IN NP + + + S+Q+A + F +D E++G+V EF +L FC LTD Q++ + L ++ I+++YF++ F+ + + + P + + I +++++ F++ D N IS+ +FR I R + + D F++ W+ + N + Y +F+
Sbjct: 169 KVFKNIKTVMKAFELIDVNKTGLVRPQELRRVLETFCMKLRDEEYEKFSKHYNIHKDTAVDYNVFLKNLSINNDLNLRYCMGNQEVSLENQQAKNSKKERLLGSASSED-IWRNYSLDEIERNFCLQLSKSYEKVEKALSAGDPCKGGYVSFNYLKIVLDTFVYQIPRRIFIQLMKRFGLKATTKINWKQFLTSFHEPQGLQVSSKGPLTKRN-------SINSRNESHKENIITKLFRHTEDHSASLKKALLIINTKPDGPITREEFRYILNCMAVKLSDSEFKELMQMLDPGDTGVVNTSMFIDLIE--ENCRMRK----TSPCTDAKTPFLLAWDSVEEIVHDTITRNLQAFYNMLRSYDLGDTGRIGRNNFKKIMHVFCPFLTNAHFIKLCS--KIQDIGSGRILYKKLLACIGIDGPPT-----VSPVLVPKDQLLSE----HLQKDEQQQPDLSERTKLTEDKTTLTKKMTTEEVIEKFKKCIQQQDPAFKKRFLDFS-KEPNGKINVHDFKKVLEDTGMPMDDDQYALLTTKIGFEKEGMSYLDFAAGF-----EDPPMRGPETTPPQPPTPSKSYVN------SHFITAEECLKLFPRRLKESFRDPYSAFFKTDADRDGIINMHDLHRLLLHLLLNLKDDEFERFLGLLGLRLSVTLNFREFQNLCEKRPWRTD-----EAPQRLIRPKQKVADSELACEQAHQYLVTKAKNRWSDLSKNFLETDNEGNGILRRRDIKNALYGFDIPLTPREFEKLWARYDTEGKGHITYQEFLQKLGI----------NYSPAV----HRPCAEDYFNFMGHFTKPQQLQEEMKELQQSTEKAVAARDKLMDRH-----QDISKAFTKTDQSKT--NYISICKMQEVLEECGCSLTEGELTHLLNSWGVSRHDNAINYLDFLRAVENSKSTGAQPKEKEESMPINFATLNPQEAVRKIQEVVESSQLA-------------LSTAFSALDKEDTGFVKATEFGQVLKDFCYKLTDNQYHYFLRKLRIHLTPY------INWKYFLQNFSCFLEETADEWAEKMPKGPPPTSPKATADRDILARLHKAVTSHYHAITQEFENFDTMKTNTISREEFRAICNRRVQILTDEQFDRLWNEMPVNAKGRLKYPDFL 1238 HSP 2 Score: 83.9593 bits (206), Expect = 2.567e-15 Identity = 126/678 (18.58%), Postives = 265/678 (39.09%), Query Frame = 3
Query: 1380 ELLSNHISNNYSSFRDAFLQINQTHKGKIPKSEFLKILNRFQFYISADQLSIILKFLDPHNEGYISYHHFLQLFEQKENLSAHKWLNSNH-----------KVVEPRKDLQLSSETIKSTISDNIGNKWSNVVHEFNLVDLNRTGYISVEDLKKIFKKLFGYLKDDDIDRLVAPLTLKDKKGRINYLELFNLLNVDTSPSDLEGLSTQIAESNDAQLKERHENHIEKMESIEKLGEARLNLMSPEELIVRIKDRIVQNRLNYHELYKKYECNDKKGGINKSQLRKMLTQFGYTLTNEQFSKISEILNFPTSSKSRIDFNGFLAIFADNRESSNDLMTDVCFLPKSNHHVNDIRGDDNRNMNCQEVEDLLRQKIVNNFPDLMIAFHKFDRSNKGYLVKSDFQNFLLGNLIMIENDQFDLLCEKLGVGEKSKLTYNDFLNRFQIR--------------------DDMVDGHKWLDS-------------KHRFNDPSTNAVPDMS----AEDVYMLLPEKIYQYFHEISPTFIALVTSGTGFIRFRNLQHLLSRLQISMSKSEMKKLWKRYDTNNRNKLDYHDFLYNLGLLEKFGCGDLMGPSAAIIQNSHNALVDHLVNQETKHQQSVDNQAVLGTHLHVEHLFEMVKRHLRVAYSDSALAFKAVANKKTLSGFFTIN 3269
+ L N +S FL+ + G + + + L F ++ + + D +G+I+Y FLQ + + H+ ++ ++ E K+LQ S+E + D + ++ ++ F D ++T YIS+ ++++ ++ L + ++ L+ + INYL+ + + ++S AQ KE+ E+ + A LN P+E + +I++ + ++L + + D G + ++ ++L F Y LT+ Q+ L + I++ FL F+ E + D + +PK + D +++ L + + +++ + F FD + + +F+ + ++ ++QFD L ++ V K +L Y DFL+RF D+ +G + S H ST +P + + L ++I + ++ + G I + L+ + + +SK E ++L +YD + K Y DF+ + LL K LM IQN+H +E + A+L + H + ++R + +Y ++ +VA+ +T+ ++IN
Sbjct: 839 QYLVTKAKNRWSDLSKNFLETDNEGNGILRRRDIKNALYGFDIPLTPREFEKLWARYDTEGKGHITYQEFLQKLGINYSPAVHRPCAEDYFNFMGHFTKPQQLQEEMKELQQSTEKAVAA-RDKLMDRHQDISKAFTKTDQSKTNYISICKMQEVLEECGCSLTEGELTHLLNSWGVSRHDNAINYLDFLR--------------AVENSKSTGAQPKEKEESM--------PINFATLN---PQEAVRKIQEVVESSQLALSTAFSALDKEDT-GFVKATEFGQVLKDFCYKLTDNQYHYFLRKLRIHLTP--YINWKYFLQNFSCFLEETADEWAEK--MPKGPPPTSPKATAD------RDILARLHKAVTSHYHAITQEFENFDTMKTNTISREEFRAICNRRVQILTDEQFDRLWNEMPVNAKGRLKYPDFLSRFSSETAATPMATGDSAVAQRGSSVPDVSEGTRSALSLPTQELRPGSKSQSHPCTPASTTVIPGTPPLQNCDPIESRLRKRIQGCWRQLLKECKEKDVARQGDINASDFLALVEKFNLDISKEECQQLIIKYDLKSNGKFAYCDFIQSCVLLLKAKESSLM--HRMKIQNAHKM-------KEAGAETPSFYSALLRIQPKIVHCWRPMRRTFK-SYDEAGTGLLSVADFRTVLRQYSIN 1469 HSP 3 Score: 76.2554 bits (186), Expect = 5.412e-13 Identity = 99/475 (20.84%), Postives = 196/475 (41.26%), Query Frame = 3
Query: 195 ISEINPIVKSQLLKNHKEILKALQTFDIECSGLVTKGELRRVLDSYCLPMTTDQYEQFLSHNSINYSKGSINYKELIDKIYDKTSAVGH--FKKNIKLPQLQRSATTICLQNKHFTLPTTRRSSGKISRSQLFXXXXXXXXXXXXXXXXWNYNAYTIDDIIPILQMQISRNPNGIMKAFKLFDFNSDYCIQRHRLKKVMENFCCPLTEQQFDKLVARFSQSQNGNVNYMEFMKGM----GIPIDNQGTIQTKNSEETINIDTRNTKGSRIFEAALIENGLLGSNELEQLLVIFRKKIEDQFSVLRKTFLVMDPNAEGFVTLEILYKILNQFAFPMSLNVFNQIMDKCAFKATGKIIWLSFLQKFQDPQSKHLNGSTIAIKSDHKCNPIQGVNSKPNI---DDLLELLSNHISNNYSSFRDAFLQINQTHKGKIPKSEFLKILNRFQFYISADQLSIILKFLDPHNEGYISYHHFL 1592
+ E+ V ++ KN K ++KA + D+ +GLV ELRRVL+++C+ + ++YE+F H +I+ K +AV + F KN+ + C+ N+ +L + + K R L S S W Y++D+I +Q+S++ + KA D + + LK V++ F + + F +L+ RF +N+ +F+ G+ + ++G + +NS I++RN + E ++ + ED + L+K L+++ +G +T E ILN A +S + F ++M TG + F+ ++ ++ C + P + D + E++ + I+ N +F + + G+I ++ F KI++ F +++ + + G I Y L
Sbjct: 159 LRELEIQVGEKVFKNIKTVMKAFELIDVNKTGLVRPQELRRVLETFCMKLRDEEYEKFSKHYNIH-----------------KDTAVDYNVFLKNLSIN--NDLNLRYCMGNQEVSLENQQAKNSKKER----------LLGSASSEDIW--RNYSLDEIERNFCLQLSKSYEKVEKALSAGDPCKGGYVSFNYLKIVLDTFVYQIPRRIFIQLMKRFGLKATTKINWKQFLTSFHEPQGLQVSSKGPLTKRNS-----INSRN------------------ESHKENIITKLFRHTEDHSASLKKALLIINTKPDGPITREEFRYILNCMAVKLSDSEFKELMQMLDPGDTGVVNTSMFIDLIEE---------NCRMRKTSPCTDAK----TPFLLAWDSVEEIVHDTITRNLQAFYNMLRSYDLGDTGRIGRNNFKKIMHVFCPFLTNAHFIKLCSKIQDIGSGRILYKKLL 566 HSP 4 Score: 73.9442 bits (180), Expect = 2.735e-12 Identity = 103/552 (18.66%), Postives = 228/552 (41.30%), Query Frame = 3
Query: 2415 NRNMNCQEVEDLLRQKIVNNFPDLMIAFHKFDRSNKGYLVKSDFQNFLLGNLIMIENDQFDLLCEKLGVGEKSKLTYNDFLNRFQIRDDMVDGHKWLDSKHRFNDPSTNAVPDMSAEDVYMLLPEKIYQYFHEISPTFIALVTSGTGFIRFRNLQHLLSRLQISMSKSEMKKLWKRYDTNNRNKLDYHDFLYNLGLLEKFGCGDLMGPSAAIIQNSHNALVDHLVNQETKHQQSVDNQAVLGTH----LHVEHLFEMVKRH--LRVAYSDSALAFKAVANKKTLSGFFTINDLNKLLSQLHMFVTNDKFVQLAARMGFEGPDDSLSYEKFCCIFQDSLNIDGQEKTVGSIRINQDLENPSKIGIEEAGNLNS------TQIASIIGKRIVDQYSEIEKTFLVMDPENSGYVSMNEFRNLLVGFCLSLTDKQFNKYRNSL---STYIVKSANTDDKIDYRYFMKGFNQKLNNLDIQXXXXXXXXXXXXXTYQQIQEHINESIKVRPKLFLNSFKSKDYASLNFISKSDFREIIERFLFTVPDSHFEKFWSNLK 4025
N ++ +V+ +L QKI + +L AF D + KS+ + + L+ + +QF + ++ + + Y FL+RF D ++G K R N + ++ + + EK+++ + F + + TG +R + L+ +L + + E +K K Y+ + +DY+ FL NL + MG + V+ E + ++ + +LG+ + + + ++R+ L+++ S + A G+ + N L +L + F+QL R G + +++++F F + Q L+ SK + + ++NS I + + + D + ++K L+++ + G ++ EFR +L + L+D +F + L T +V ++ D I+ M+ ++ A + + ++E ++++I + F N +S D I +++F++I+ F + ++HF K S ++
Sbjct: 52 NPTLSSLDVKRILFQKITDRGDELQKAFQLLDTGQNLTVSKSELRRIITDFLMPLTREQFQDVLAQIPLSTSGTVPYLAFLSRFGGIDLYINGIK------RGGGNEMNCC--RTLRELEIQVGEKVFKNIKTVMKAFELIDVNKTGLVRPQELRRVLETFCMKLRDEEYEKFSKHYNIHKDTAVDYNVFLKNLSINNDLNLRYCMG--------------NQEVSLENQQAKNSKKERLLGSASSEDIWRNYSLDEIERNFCLQLSKSYEKVEKALSAGDPCKGGYVSFNYLKIVLDTFVYQIPRRIFIQLMKRFGLKA-TTKINWKQFLTSFHEP----------------QGLQVSSKGPLTKRNSINSRNESHKENIITKLFRHTEDHSASLKKALLIINTKPDGPITREEFRYILNCMAVKLSDSEFKELMQMLDPGDTGVVNTSMFIDLIEENCRMR-----------KTSPCTDAKTPFLLAWDSVEEIVHDTITRNLQAFYNMLRSYDLGDTGRIGRNNFKKIMHVFCPFLTNAHFIKLCSKIQ 553 HSP 5 Score: 68.1662 bits (165), Expect = 1.708e-10 Identity = 49/212 (23.11%), Postives = 97/212 (45.75%), Query Frame = 3
Query: 4161 TDEMDTDESIRKNLERLLNRNWQSIFNKCKAIDQAINECQTAEGSIPIDDFVYILSEYDDSLSAENLAAINL-YKTCKNGRFIKYGDLIRSIVL---SSRGSLISNENVTEVQKKFLNVGIKTTNSDNSKDYFPQLFKLRRQILENWKDLRKNFLQSDKKQNGAVSGNIFMTIMKTVCGQLSADLFYHLMKIYDQDGTGNFHYKRFIEDFLK 4784
T + + I L + + W+ + +CK D A +G I DF+ ++ +++ +S E + + Y NG+F Y D I+S VL + SL+ + K G +T + ++ L +++ +I+ W+ +R+ F D+ G +S F T+++ LS + F+H+++ YD+ + Y F+ FL+
Sbjct: 1304 TPPLQNCDPIESRLRKRIQGCWRQLLKECKEKDVA------RQGDINASDFLALVEKFNLDISKEECQQLIIKYDLKSNGKFA-YCDFIQSCVLLLKAKESSLMHRMKIQNAHK-MKEAGAETPS------FYSALLRIQPKIVHCWRPMRRTFKSYDEAGTGLLSVADFRTVLRQYSINLSEEEFFHILEYYDKTLSSKISYNDFLRAFLQ 1501 HSP 6 Score: 58.151 bits (139), Expect = 1.781e-7 Identity = 129/719 (17.94%), Postives = 267/719 (37.13%), Query Frame = 3
Query: 1002 IENGLLGSNELEQLLVIFRKKIEDQFSVLRKTFLVMDPNAEGFVTLEILYKILNQFAFPMSLNVFNQIMDKCAFKATGKIIWLSFLQKFQ---DPQSKHLNGSTIAIKSDHKCNPIQGVNSKPNIDDLLE---LLSNHISNNYSSFRDAFLQINQTHKGKIPKSEFLKILNRFQFYISADQLSIIL-KFLDPHNEGYISYHHFLQLFEQKENLSAH-----KWLNSNHKVVEPRKDLQLSSETIKSTISDNIGNKWSNVVHEFNLVDLNRTGYISVEDLKKIFKKLFGYLKDDDIDRLVAPLTLKDKKGRINYLELFNLLNVDTSPSDLEGLSTQIAESNDAQLKERHENHIEKMESIEKLGEARLNLMSPEELIVRIKDRIVQNRLN------YHELYKKYECND--KKGGINKSQLRKMLTQFGYTLTNEQFSKISEILNFPTSSKSRIDFNGFLAIFADNRESSNDLMTDVCFLPKSNHHVNDIR----------------GDDNRN-----------------MNCQEVEDLLRQKIVNNFPDLMIAFHKFDRSNKGYLVKSDFQNFLLGNLIMIENDQFDLLCEKLGVGEKSKLTYNDFLNR----FQIRDDMVDGHKWLDSKHRFNDPSTNAVPDMSAEDVYMLLPEKIYQYFHEISPTFIALVTSGTGFIRFRNLQHLLSRLQISMSKSEMKKLWKRYDTNNRNKLDYHDFL 2987
+ + L + Q LV K ++++S L K FL D G + + L F P++ F ++ + + G I + FLQK P H P Q + E + + + + AF + +Q+ I + ++L ++ +L+ +L + ++ I+Y FL+ E ++ A + + N + P++ ++ E ++S+ + F+ +D TG++ + ++ K L D+ + +K RI+ N L+ S + E+ D E EK+ + P DR + RL+ YH + +++E D K I++ + R + + LT+EQF ++ P ++K R+ + FL+ F+ + ++ + T + + V D+ G +++ NC +E LR++I + L+ + D + +G + SDF + + I ++ L K + K Y DF+ + ++ + + + H+ + SA + + KI + + TF + +GTG + + + +L + I++S+ E + + YD +K+ Y+DFL
Sbjct: 827 VADSELACEQAHQYLVT---KAKNRWSDLSKNFLETDNEGNGILRRRDIKNALYGFDIPLTPREFEKLWARYDTEGKGHITYQEFLQKLGINYSPAVHRPCAEDYFNFMGHFTKPQQLQEEMKELQQSTEKAVAARDKLMDRHQDISKAFTKTDQSKTNYISICKMQEVLEECGCSLTEGELTHLLNSWGVSRHDNAINYLDFLRAVENSKSTGAQPKEKEESMPINFATLNPQEAVRKIQEVVESS--------QLALSTAFSALDKEDTGFVKATEFGQVLKDFCYKLTDNQYHYFL-------RKLRIHLTPYINWKYF------LQNFSCFLEETAD--------------EWAEKMPKG-----PPPTSPKATADRDILARLHKAVTSHYHAITQEFENFDTMKTNTISREEFRAICNRRVQILTDEQFDRLWN--EMPVNAKGRLKYPDFLSRFS-SETAATPMATGDSAVAQRGSSVPDVSEGTRSALSLPTQELRPGSKSQSHPCTPASTTVIPGTPPLQNCDPIESRLRKRIQGCWRQLLKECKEKDVARQGDINASDFLALVEKFNLDISKEECQQLIIKYDLKSNGKFAYCDFIQSCVLLLKAKESSLMHRMKIQNAHKMKEAGAETPSFYSA---LLRIQPKIVHCWRPMRRTFKSYDEAGTGLLSVADFRTVLRQYSINLSEEEFFHILEYYDKTLSSKISYNDFL 1496
BLAST of EF-hand calcium-binding domain-containing protein 6 vs. Ensembl Human
Match: EFCAB6 (EF-hand calcium binding domain 6 [Source:HGNC Symbol;Acc:HGNC:24204]) HSP 1 Score: 52.373 bits (124), Expect = 1.193e-6 Identity = 25/81 (30.86%), Postives = 47/81 (58.02%), Query Frame = 3
Query: 147 NSTIKDANYKMDPKLQISEINPIVKSQLLKNHKEILKALQTFDIECSGLVTKGELRRVLDSYCLPMTTDQYEQFLSHNSIN 389
N + +M+ + E+ V ++ KN K ++KA + D+ +GLV ELRRVL+++C+ + ++YE+F H +I+
Sbjct: 37 NGIKRGGGNEMNCCRTLRELEIQVGEKVFKNIKTVMKAFELIDVNKTGLVRPQELRRVLETFCMKLRDEEYEKFSKHYNIH 117
BLAST of EF-hand calcium-binding domain-containing protein 6 vs. Ensembl Xenopus
Match: immp2l (inner mitochondrial membrane peptidase subunit 2 [Source:Xenbase;Acc:XB-GENE-1010851]) HSP 1 Score: 445.277 bits (1144), Expect = 2.606e-130 Identity = 337/1312 (25.69%), Postives = 608/1312 (46.34%), Query Frame = 3
Query: 180 DPKLQISEINPIVKSQLLKNHKEILKALQTFDIECSGLVTKGELRRVLDSYCLPMTTDQYEQFLSHNSINYSKGSINYKELIDKIYDKTSAVGHFKKNIKLPQLQRSATTICLQNKHFTLPTTRRSSGKISRSQLFXXXXXXXXXXXXXXXXWNYNAYTIDDIIPILQMQISRNPNGIMKAFKLFDFNSDYCIQRHRLKKVMENFCCPLTEQQFDKLVARFSQSQNGNVNYMEFMKGMGIPIDNQGTIQTKNSEETIN---IDTRNTKGSRIFEAALIENGLLGSNELEQLLVIFRKKIEDQFSVLRKTFLVMDPNAEGFVTLEILYKILNQFAFPMSLNVFNQIMDKCAFKATGKIIWLSFLQKFQDPQSKHLNGSTIAIKSDHKCNPIQGVNSKPNIDDLLELLSNHISNNYSSFRDAFLQINQTHKGKIPKSEFLKILNRFQFYISADQLSIILKFLDPHNEGYISYHHFLQLFEQKENLSAHKWLNSNHKVVEPRKD--LQLSSETIKSTISDNIGNKWSNVVHEFNLVDLNRTGYISVEDLKKIFKKLFGYLKDDDIDRLVAPLTLKDKKGRINYLELFNLLNVDTSPS-DLEGLSTQIAESNDAQLKERHENHIEKMESIEKLGEARLNLMSPEELIVRIKDRIVQNRLNYHELYKKYECNDKKGGINKSQLRKMLTQFGYTLTNEQFSKISEILNFPTSSKSRIDFNGFLAIFADNRESSNDLMTDVCFLPKSNHHVNDIRGDDNRNMNCQEVEDLLRQKIVNNFPDLMIAFHKFDRSNKGYLVKSDFQNFLLGNLIMIENDQFDLLCEKLGVGEKSKLTYNDFLNRFQIRDDMVDGHKWLDSKHRFNDPSTNAVPDMSAEDVYMLLPEKIYQYFHEISPTFIALVTSGTGFIRFRNLQHLLSRLQISMSKSEMKKLWKRYDTNNRNKLDYHDFLYNLGLLEKFGCGDLMGPSAAIIQNSHNALVDHLVNQETKHQQSVDNQAVLGTHLHVEHLFEM------VKRHLRVAYSDSALAFKAVANKKTLSGFFTINDLNKLLSQLHMFVTNDKFVQLAARMGFEGPDDSLSYEKFCCIFQDSLNIDGQEKTVGSI-RINQDLENPSKIGIEEAGNLNSTQIASIIGKRIVDQYSEIEKTFLVMDPENSGYVSMNEFRNLLVGFCLSLTDKQFNKYRNSLSTYIVKSANTDDKIDYRYFMKGFN----QKLNNLDIQXXXXXXXXXXXXXTYQQIQEHINESIKVRPKLFLNSFKSKDYASLNFISKSDFREIIERFLFTVPDSHFEKFWSNLKKNEFDNVNYGEFV 4064
DP L EI I+ ++ +N +++ A T D E + VT+GE RVL ++ LP+T Q+ + L +N + G+I Y + + K L+ S++ C + + +RS +Q+ +++++ L+ +S+N ++++ +LFD+N + IQ+ L++++EN C + +F+KL R+ + ++Y E ++ +G+ ++ +N +N + K ++ ++ + RKKI + L K F D + G+V+ E L ++N F FP+ F ++M++ A K T +I W FL++FQ P+S NG TI +K +H+ NP++G + + + +L+ L H + Y S + AFL +++ KG+I + E +I++ F ++ Q ++ LDP + G+ISY FL LFE KE++S HKWL H + P K+ + L E + + + + I + W++ D G + + K+I + L D + L G I+Y++ + V TS + DL G+ST+I + + + R ++ +M+ IE + M EE+I ++KD ++ + E + + KG I+K RK+L + L +QF +++E L F +K + + F+++F D + D SNHHVND + M+ ++ LR K+ + DL AF+K D + G + DF++ L + +I+ +F L LG+ S L Y +FL+ FQ V WL S H + + D++ E + L K +H+++ TF + + G G ++ ++L+++L R + + E +KLW RYD + L +++FL LG+ F GD GPS I +++ L H NQ K Q+ +D H H +M +K R Y D + AF + K G +++DL +L L+ + +++F L R+ + D+ LSY F + DG+ G R E P K+ ++ ++ + + +V + + K F D + +G + +E +L FC L+D+QF + L + ID++ F+ F+ + ++ + A + +T + I HI E + R F S DYA++N +SK DFRE+ + + D FE W+ L N + N+ Y EF+
Sbjct: 51 DPTLTPLEIEGIIYRRVNENEEDVTNAFLTLDTEQNFTVTRGEFYRVLQNFILPLTESQFNELLEKIPVN-NNGTIPYLDFLAKF------------------LRTSSSATCAR-------SVKRSWSCSQANQVL----------------------SLNELEIRLKNTVSKNLKNVVRSCRLFDYNQNGQIQKQELRRILENCCFKMKTAEFEKLWNRYCIGKKNTLDYRELLRNLGVNVELHNKPTAENVASALNREAVQREQQKEKNCRPPPPLKELSAEDYTIDDIEAALRKKISASYPNLVKAFRAFDGSHSGYVSAEALQSVINNFIFPLPNGTFQKLMNRLAIKCTDRIGWEQFLRRFQGPESM-ANGQTIPMKPNHRVNPVRGESPVVSSEHILQKLQRHFQDAYPSLKQAFLVLDEGRKGRISRKELRRIVDCMMFRVTDAQFKELMIILDPQHTGFISYLQFLDLFEDKESVSGHKWL---HNMRTPLKETPVTLEWEKMGNILCEKIEHNWADFSKAMKSCDSAGCGMVDKDQFKRILQSFCPPLSDRHYNMLCEQYN-DGANGYISYMDFLRNVGVCTSAAGDLNGVSTEIVHGSQLREELRQDDLSARMKEIESQATQLVKKMPVEEVIGKLKDCVIGQEFSPRESFLAF-AKQHKGRISKKDFRKVLREHEIHLDEDQFKRLTEKLGF---TKEELSYLDFVSLFEDPTGNGPGAALDT----GSNHHVNDTKF---HYMSAEDCLAQLRDKLQQGYGDLYSAFYKIDSNRDGLVTMHDFRHLLDSFMFIIKPQEFQRLLGLLGLNLSSTLNYKEFLHLFQKPQSPV-YPPWLQSSH--SPKQSMGGEDLACEQAHYYLVTKAQGRWHDMAKTFCEIDSEGNGIVQKKDLRNVLFRFYLPIHPKEFEKLWGRYDPEGKGFLTHNEFLEKLGI--SFASGD-SGPSKQITEDNRACLEGHYNNQ-LKIQEEMD-----AFHKHQTKALDMKDIEQQIKDKFRDYYHDFSAAFAKIDRNK--DGQISVHDLRAMLQSLNFHLDDEQFANLLYRLRIKVTDNKLSYPDFLRMID-----DGRASKYGQRQRPVSAAETPPKLSLQ--------KVLLKLKEAVVSSHDALYKAFSSFDRDGTGTIKASELHQILDSFCFKLSDRQFGYLLSKLPL------GEEQTIDWKAFLHHFSTSHPEPSSSWVERVQHATRPRSTQGLTMKDILNHIREVVTARFYTVAQDFISLDYANINAVSKEDFRELFNKDFMLLTDEQFENLWNTLPLNPYGNLKYHEFL 1265 HSP 2 Score: 192.971 bits (489), Expect = 1.054e-48 Identity = 235/1213 (19.37%), Postives = 492/1213 (40.56%), Query Frame = 3
Query: 165 ANYKMDPKLQISEINPIVKS----QLLKNH-----KEILKALQTFDIECSGLVTKGELRRVLDSYCLPMTTDQYEQFLSHNSINYSKGSINYKELIDKIYDKTSAVGH-FKKNIKLPQLQRSATTICLQNKHFTLPTTRRSSGKISRSQLFXXXXXXXXXXXXXXXXWNYNAYTIDDIIPILQMQISRNPNGIMKAFKLFDFNSDYCIQRHRLKKVMENFCCPLTEQQFDKLVARFSQSQNGNVNYMEFMKGMGIPIDNQGTIQTKNSEETINIDTR-----NTKGSRIFEAALIENGLLGSNELEQLLVIFRKKIEDQFSVLRKTFLVMDPNAEGFVTLEILYKILNQFAFPMSLNVFNQIMDKCAFKATGKIIWLSFLQKFQDPQSKHLNGSTIAIKSDHKCNPIQGVNSKPNIDDLLELLSNHISNNYSSFRDAFLQINQTHKGKIPKSEFLKILNRFQFYISADQLSIILKFLDPHNEGYISYHHFLQLFEQKENLSAHKWLNSNHKVVEPRKDLQLSSETIKSTISDNIGNKWSNVVHEFNLVDLNRTGYISVEDLKKIFKKLFGYLKDDDIDRLVAPLTLKDKKGRINYLELFNLLNVDTSPSDLEGLSTQIAESNDAQLKERHENHIEKMESIEKLGEARLNLMSPEELIVRIKDRIVQNRLNYHELYKKYECNDKKGGINKSQLRKMLTQFGYTLTNEQFSKISEILNFPTSSKSRIDFNGFLAIFADNRESSNDLMTDVCFLPKSNHHVNDIRGDDN-RNMNCQEVEDLLRQKIVNNFPDLMIAFHKFDRSNKGYLVKSDFQNFLLGNLIMIENDQFDLLCEKLGVGEKSKLTYNDFLNRFQIRDDMVDGHKWLDSKHRFNDPSTNAVPDMSAEDVYMLLPEKIYQYFHEISPTFIALVTSGTGFIRFRNLQHLLSRLQISMSKSEMKKLWKRYDTNNRNKLDYHDFLYNLGLLEKFGCGDLM--------GPSAAIIQNSHNALVDH------------------LVNQETKHQQSVDNQAVLGTHLHVEHLFEMVKRHLRVAYSDSALAFKAVANKKTLSGFFTINDLNKLLSQLHMFVTNDKFVQLAARMGFEGPDDSLSYEKFCCIFQD-SLNIDGQEKTVGSIRINQDLENPSKIGIEEAGNLNSTQIASIIGKRIVDQYSEIEKTFLVMDPENSGYVSMNEFRNLLVGFCLSLTDKQF 3674
N++++P + +P+V S Q L+ H + +A D G +++ ELRR++D +T Q+++ + ++ G I+Y + +D DK S GH + N++ P L+ + T+ W + + IL +I N KA K D + + + K+++++FC PL+++ ++ L +++ NG ++YM+F++ +G+ G + ++E R + +R+ E L+ +E+++ + + Q R++FL +G ++ + K+L + + + F ++ +K F ++ +L F+ F+DP + + G+ + S+H N + + +D L L + + Y AF +I+ G + +F +L+ F F I + +L L + ++Y FL LF++ ++ WL S+H + L+ E + +W ++ F +D G + +DL+ + + + + + ++L + KG + + E L + + D G S QI E N A L+ + N ++ E ++ + + + +++ +IKD+ ++ + K + N K G I+ LR ML + L +EQF+ + L + +++ + FL + D R S K + + ++ Q+V L++ +V++ L AF FDR G + S+ L + + QF L KL +GE+ + + FL+ F W++ P + ++ +D+ + E + F+ ++ FI+L + + + + L ++ + ++ + + LW N L YH+F L +FG GD + G + I +AL + ++N+ Q ++ E + ++ L+ + + A + ++T G T ND + +L Q + ++ +L + + SY F F++ L EK+ Q P G + LN+ I +++D + + + FL D +G++S+ EF+ +L L+L++ +F
Sbjct: 384 PNHRVNP---VRGESPVVSSEHILQKLQRHFQDAYPSLKQAFLVLDEGRKGRISRKELRRIVDCMMFRVTDAQFKELMIILDPQHT-GFISYLQFLDLFEDKESVSGHKWLHNMRTP-LKETPVTL----------------------------------------EW-------EKMGNILCEKIEHNWADFSKAMKSCDSAGCGMVDKDQFKRILQSFCPPLSDRHYNMLCEQYNDGANGYISYMDFLRNVGVCTSAAGDLNGVSTEIVHGSQLREELRQDDLSARMKEIESQATQLVKKMPVEEVIGKLKDCVIGQEFSPRESFLAFAKQHKGRISKKDFRKVLREHEIHLDEDQFKRLTEKLGFTKE-ELSYLDFVSLFEDP-TGNGPGAALDTGSNHHVNDTKF--HYMSAEDCLAQLRDKLQQGYGDLYSAFYKIDSNRDGLVTMHDFRHLLDSFMFIIKPQEFQRLLGLLGLNLSSTLNYKEFLHLFQKPQSPVYPPWLQSSHSPKQSMGGEDLACEQAHYYLVTKAQGRWHDMAKTFCEIDSEGNGIVQKKDLRNVLFRFYLPIHPKEFEKLWGRYD-PEGKGFLTHNEFLEKLGISFASGD-SGPSKQITEDNRACLEGHYNNQLKIQEEMDAFHKHQTKALDMKDIEQQIKDKFRDYYHDFSAAFAKIDRN-KDGQISVHDLRAMLQSLNFHLDDEQFANLLYRLRIKVTD-NKLSYPDFLRMIDDGRAS------------KYGQRQRPVSAAETPPKLSLQKVLLKLKEAVVSSHDALYKAFSSFDRDGTGTIKASELHQILDSFCFKLSDRQFGYLLSKLPLGEEQTIDWKAFLHHFSTSHPEPSS-SWVERVQHATRPRSTQ--GLTMKDILNHIREVVTARFYTVAQDFISLDYANINAVSKEDFRELFNKDFMLLTDEQFENLWNTLPLNPYGNLKYHEF------LSQFGGGDALVSPYEKTAGDQSEFIYKPASALAGNHTPPESQAGIRRPKTAPAILNKPKTDTQRPKTAGASSRLVNCEQIEARLRGSLQKVWQEVLRACRDYDPERT--GEITTNDFSGVLKQFNAETNEEELERLTVKYDSRN-NGKFSYADF---FRNIMLGPKPLEKSPLHRMKVQKTRVPISTGPDGPCFLNAMM---RIQPKVLDCWRPMRRAFLSFDEARTGFISVQEFKQVLRKSGLNLSEDEF 1506 HSP 3 Score: 89.7373 bits (221), Expect = 4.247e-17 Identity = 111/552 (20.11%), Postives = 229/552 (41.49%), Query Frame = 3
Query: 2436 EVEDLLRQKIVNNFPDLMIAFHKFDRSNKGYLVKSDFQNFLLGNLIMIENDQFDLLCEKLGVGEKSKLTYNDFLNRFQIRDDMVDGHKWLDSKHRFNDPSTNAVPDMSAEDVYMLLPEKIYQYFHEISPTFIALVTSGTGFIRFRNLQHLLSRLQISMSKSEMKKLWKRYDTNNRNKLDYHDFLYNLGLLEKFGCGDLMGPSAAIIQNSHNALVDHLVNQETKHQQSVDNQAVL----GTHLHVEHLFEMVKRHLRVAYSDSALAFKAVANKKTLSGFFTINDLNKLLSQLHMFVTNDKFVQLAARMGFEGPDDSLSYEKFCCIFQDSLNI-DGQEKTVGSIRINQDLENPSKIGIEEAGNLNSTQIASIIGKRIVDQYSEIEKTFLVMDPENSGYVSMNEFRNLLVGFCLSLTDKQFNKYRNSLSTYIVKSANTDDKIDYRYFMKGFNQK---LNNLDIQXXXXXXXXXXXXXTYQQIQEHINESIKVRPKLFLNSFKSKDYASLNFISKSDFREIIERFLFTVPDSHFEKFWSNLKKNEFDNVNYGEFVK 4067
E+E ++ +++ N D+ AF D + + +F L ++ + QF+ L EK+ V + Y DFL +F + + K ++ N V +S ++ + L + + + + + G I+ + L+ +L M +E +KLW RY +N LDY + L NLG+ ++ + +N +AL V +E + +++ L ++ + +++ + +Y + AF+A + SG+ + L +++ + N F +L R+ + D + +E+F FQ ++ +GQ T+ ++ N + NP + E+ ++S I + + D Y +++ FLV+D G +S E R ++ +TD QF + I+ I Y F+ F K + + +M + ++++ + E I+ F + KS D A + K F+ I++ F + D H+ ++Y +F++
Sbjct: 58 EIEGIIYRRVNENEEDVTNAFLTLDTEQNFTVTRGEFYRVLQNFILPLTESQFNELLEKIPVNNNGTIPYLDFLAKFLRTSSSATCARSV--KRSWSCSQANQV--LSLNELEIRLKNTVSKNLKNVVRSCRLFDYNQNGQIQKQELRRILENCCFKMKTAEFEKLWNRYCIGKKNTLDYRELLRNLGV-------NVELHNKPTAENVASALNREAVQREQQKEKNCRPPPPLKELSAEDYTIDDIEAALRKKISASYPNLVKAFRAFDG--SHSGYVSAEALQSVINNFIFPLPNGTFQKLMNRLAIKC-TDRIGWEQFLRRFQGPESMANGQ--TI-PMKPNHRV-NPVR---GESPVVSSEHILQKLQRHFQDAYPSLKQAFLVLDEGRKGRISRKELRRIVDCMMFRVTDAQFKEL------MIILDPQHTGFISYLQFLDLFEDKESVSGHKWLHNMRTPLKETPVTLEWEKMGNILCEKIEHNWADFSKAMKSCDSAGCGMVDKDQFKRILQSFCPPLSDRHYNMLCEQYNDGANGYISYMDFLR 582 HSP 4 Score: 77.0258 bits (188), Expect = 3.075e-13 Identity = 48/202 (23.76%), Postives = 93/202 (46.04%), Query Frame = 3
Query: 4182 ESIRKNLERLLNRNWQSIFNKCKAIDQAINECQTAEGSIPIDDFVYILSEYDDSLSAENLAAINL-YKTCKNGRFIKYGDLIRSIVLSSRGSLISNENVTEVQKKFLNVGIKTTNSDNSKDYFPQLFKLRRQILENWKDLRKNFLQSDKKQNGAVSGNIFMTIMKTVCGQLSADLFYHLMKIYDQDGTGNFHYKRFIEDFLK 4784
E I L L + WQ + C+ D G I +DF +L +++ + E L + + Y + NG+F Y D R+I+L + S + +VQK + + + + + +++ ++L+ W+ +R+ FL D+ + G +S F +++ LS D F+H++ +D+D Y F+ FL+
Sbjct: 1342 EQIEARLRGSLQKVWQEVLRACRDYDPE------RTGEITTNDFSGVLKQFNAETNEEELERLTVKYDSRNNGKF-SYADFFRNIMLGPKPLEKSPLHRMKVQK----TRVPISTGPDGPCFLNAMMRIQPKVLDCWRPMRRAFLSFDEARTGFISVQEFKQVLRKSGLNLSEDEFFHILGFFDKDLKAKVSYNDFLRAFLR 1532 HSP 5 Score: 73.559 bits (179), Expect = 2.901e-12 Identity = 156/834 (18.71%), Postives = 316/834 (37.89%), Query Frame = 3
Query: 642 YNAYTIDDIIPILQMQISRNPNGIMKAFKLFDFNSDYCIQRHRLKKVMENFCCPLTEQQFDKLVARFSQSQNGNVNYMEFMKGMGIPIDNQGTIQTKNSEETINIDTRNTKGSRIFEAALIENGLLGSNEL--EQLLVIFRKKIEDQFSVLRKTFLVMDPNAEGFVTLEILYKILNQFAFPMSLNVFNQIMDKCAFKATGKIIWLSFLQKFQDPQSKHLNGSTIAIKSDHKCNPIQGVNSKPNIDDLLELLSNH-----------------ISNNYSSFRDAFLQINQTHKGKIPKSEFLKILNRFQFYISADQLSIILKFLDPH-NEGYISYHHFLQLFEQKENLSAHKWLNSNHKVVEPRKDLQLSSETIKSTISDNIGNKWSNVVHEFNLVDLNRTGYISVEDLKKIFKKLFGYLKDDDIDRLVA--PLTLKDKKGRINYLELFNLLNVDTSPSDLEGL--STQIAESNDAQLKERHENHIEKMESIE---------KLGEARLNLMSPEELIVRI-KDRIVQNRLNYHELYKKYECNDKKGGINKSQLRKMLTQFG-----YTLTNEQFSKISEILNFPTSSKSRIDFNGFLAIFADNRESSNDLM---TDVCFL--PKSNHHVNDIRGDDNRNMNCQEVEDLLRQKIVNNFPDLMIAFHKFDRSNKGYLVKSDFQNFLLGNLIMIENDQFDLLCEKLGVGEKSKLTYNDFLNRFQIRDDMVDGHKWLDSK--HRFNDPSTNAVPDMSAED------VYMLLPEKIYQYFHEISPTFIALVTSGTGFIRFRNLQHLLSRLQISMSKSEMKKLWKRYDTNNRNKLDYHDFL 2987
++ + +D + L+ ++ + + AF D N D + H + ++++F + Q+F +L+ + + +NY EF+ P Q + +++ +G +L EQ K + ++ + KTF +D G V + L +L +F P+ F ++ + + G + FL+K + +G + I D++ N++ I + ++ H + Y F AF +I++ G+I + +L F++ +Q + +L L + +SY FL++ + A K+ V +LS + + + + + + + F+ D + TG I +L +I L D L++ PL + +L F+ + + S S +E + +T+ + +K+ NHI ++ + L A +N +S E+ KD ++ + L+ N + + L+QFG + + SE + P S+ LA ES + T L PK++ G +R +NC+++E LR + + +++ A +D G + +DF L ++ + L K K +Y DF + G K L+ HR T VP + D M + K+ + + F++ + TGFI + + +L + +++S+ E + +D + + K+ Y+DFL
Sbjct: 738 FHYMSAEDCLAQLRDKLQQGYGDLYSAFYKIDSNRDGLVTMHDFRHLLDSFMFIIKPQEFQRLLGLLGLNLSSTLNYKEFLHLFQKP---QSPVYPP-----------------WLQSSHSPKQSMGGEDLACEQAHYYLVTKAQGRWHDMAKTFCEIDSEGNGIVQKKDLRNVLFRFYLPIHPKEFEKLWGRYDPEGKGFLTHNEFLEKLGISFASGDSGPSKQITEDNRACLEGHYNNQLKIQEEMDAFHKHQTKALDMKDIEQQIKDKFRDYYHDFSAAFAKIDRNKDGQISVHDLRAMLQSLNFHLDDEQFANLLYRLRIKVTDNKLSYPDFLRMIDDGR---ASKYGQRQRPVSAAETPPKLSLQKVLLKLKEAVVSSHDALYKAFSSFDRDGTGTIKASELHQILDSFCFKLSDRQFGYLLSKLPLGEEQTIDWKAFLHHFSTSHPEPSSSWVERVQHATRPRSTQGLTMKDIL-NHIREVVTARFYTVAQDFISLDYANINAVSKEDFRELFNKDFMLLTDEQFENLWNTLPLNP----YGNLKYHEFLSQFGGGDALVSPYEKTAGDQSEFIYKPASA---------LAGNHTPPESQAGIRRPKTAPAILNKPKTDTQRPKTAGASSRLVNCEQIEARLRGSLQKVWQEVLRACRDYDPERTGEITTNDFSGVLKQFNAETNEEELERLTVKYDSRNNGKFSYADFFRNIML------GPKPLEKSPLHRMKVQKTR-VPISTGPDGPCFLNAMMRIQPKVLDCWRPMRRAFLSFDEARTGFISVQEFKQVLRKSGLNLSEDEFFHILGFFDKDLKAKVSYNDFL 1527
BLAST of EF-hand calcium-binding domain-containing protein 6 vs. Ensembl Xenopus
Match: immp2l (inner mitochondrial membrane peptidase subunit 2 [Source:Xenbase;Acc:XB-GENE-1010851]) HSP 1 Score: 420.624 bits (1080), Expect = 8.361e-122 Identity = 327/1310 (24.96%), Postives = 595/1310 (45.42%), Query Frame = 3
Query: 180 DPKLQISEINPIVKSQLLKNHKEILKALQTFDIECSGLVTKGELRRVLDSYCLPMTTDQYEQFLSHNSINYSKGSINYKELIDKIYDKTSAVGHFKKNIKLPQLQRSATTICLQNKHFTLPTTRRSSGKISRSQLFXXXXXXXXXXXXXXXXWNYNAYTIDDIIPILQMQISRNPNGIMKAFKLFDFNSDYCIQRHRLKKVMENFCCPLTEQQFDKLVARFSQSQNGNVNYMEFMKGMGIPIDNQGTIQTKNSEETIN---IDTRNTKGSRIFEAALIENGLLGSNELEQLLVIFRKKIEDQFSVLRKTFLVMDPNAEGFVTLEILYKILNQFAFPMSLNVFNQIMDKCAFKATGKIIWLSFLQKFQDPQSKHLNGSTIAIKSDHKCNPIQGVNSKPNIDDLLELLSNHISNNYSSFRDAFLQINQTHKGKIPKSEFLKILNRFQFYISADQLSIILKFLDPHNEGYISYHHFLQLFEQKENLSAHKWLNSNHKVVEPRKDLQLSSETIKSTISDNIGNKWSNVVHEFNLVDLNRTGYISVEDLKKIFKKLFGYLKDDDIDRLVAPLTLKDKKGRINYLELFNLLNVDTSPS-DLEGLSTQIAESNDAQLKERHENHIEKMESIEKLGEARLNLMSPEELIVRIKDRIVQNRLNYHELYKKYECNDKKGGINKSQLRKMLTQFGYTLTNEQFSKISEILNFPTSSKSRIDFNGFLAIFADNRESSNDLMTDVCFLPKSNHHVNDIRGDDNRNMNCQEVEDLLRQKIVNNFPDLMIAFHKFDRSNKGYLVKSDFQNFLLGNLIMIENDQFDLLCEKLGVGEKSKLTYNDFLNRFQIRDDMVDGHKWLDSKHRFNDPSTNAVPDMSAEDVYMLLPEKIYQYFHEISPTFIALVTSGTGFIRFRNLQHLLSRLQISMSKSEMKKLWKRYDTNNRNKLDYHDFLYNLGLLEKFGCGDLMGPSAAIIQNSHNALVDHLVNQETKHQQSVDNQAVLGTHLHVEHLFEM------VKRHLRVAYSDSALAFKAVANKKTLSGFFTINDLNKLLSQLHMFVTNDKFVQLAARMGFEGPDDSLSYEKFCCIFQDSLNIDGQEKTVGSI-RINQDLENPSKIGIEEAGNLNSTQIASIIGKRIVDQYSEIEKTFLVMDPENSGYVSMNEFRNLLVGFCLSLTDKQFNKYRNSLSTYIVKSANTDDKIDYRYFMKGFN----QKLNNLDIQXXXXXXXXXXXXXTYQQIQEHINESIKVRPKLFLNSFKSKDYASLNFISKSDFREIIERFLFTVPDSHFEKFWSNLKKNEFDNVNYGEFV 4064
DP L EI I+ ++ +N +++ A T D E + VT+GE RVL ++ LP+T Q+ + L +N + G+I Y + + K L+ S++ C + + +RS +Q+ +++++ L+ +S+N ++++ +LFD+N + IQ+ L++++EN C + +F+KL R+ + ++Y E ++ +G+ ++ +N +N + K ++ ++ + RKKI + L K F D + G+V+ E L ++N F FP+ F ++M++ A K T +I W FL++FQ P+S NG TI +K +H+ NP++G + + + +L+ L H + Y S + AFL +++ KG+I + E +I++ F ++ Q ++ LDP + G+ISY FL LFE KE++ + + + + I + W++ D G + + K+I + L D + L G I+Y++ + V TS + DL G+ST+I + + + R ++ +M+ IE + M EE+I ++KD ++ + E + + KG I+K RK+L + L +QF +++E L F +K + + F+++F D + D SNHHVND + M+ ++ LR K+ + DL AF+K D + G + DF++ L + +I+ +F L LG+ S L Y +FL+ FQ V WL S H + + D++ E + L K +H+++ TF + + G G ++ ++L+++L R + + E +KLW RYD + L +++FL LG+ F GD GPS I +++ L H NQ K Q+ +D H H +M +K R Y D + AF + K G +++DL +L L+ + +++F L R+ + D+ LSY F + DG+ G R E P K+ ++ ++ + + +V + + K F D + +G + +E +L FC L+D+QF + L + ID++ F+ F+ + ++ + A + +T + I HI E + R F S DYA++N +SK DFRE+ + + D FE W+ L N + N+ Y EF+
Sbjct: 51 DPTLTPLEIEGIIYRRVNENEEDVTNAFLTLDTEQNFTVTRGEFYRVLQNFILPLTESQFNELLEKIPVN-NNGTIPYLDFLAKF------------------LRTSSSATCAR-------SVKRSWSCSQANQVL----------------------SLNELEIRLKNTVSKNLKNVVRSCRLFDYNQNGQIQKQELRRILENCCFKMKTAEFEKLWNRYCIGKKNTLDYRELLRNLGVNVELHNKPTAENVASALNREAVQREQQKEKNCRPPPPLKELSAEDYTIDDIEAALRKKISASYPNLVKAFRAFDGSHSGYVSAEALQSVINNFIFPLPNGTFQKLMNRLAIKCTDRIGWEQFLRRFQGPESM-ANGQTIPMKPNHRVNPVRGESPVVSSEHILQKLQRHFQDAYPSLKQAFLVLDEGRKGRISRKELRRIVDCMMFRVTDAQFKELMIILDPQHTGFISYLQFLDLFEDKESV-------------------------MGNILCEKIEHNWADFSKAMKSCDSAGCGMVDKDQFKRILQSFCPPLSDRHYNMLCEQYN-DGANGYISYMDFLRNVGVCTSAAGDLNGVSTEIVHGSQLREELRQDDLSARMKEIESQATQLVKKMPVEEVIGKLKDCVIGQEFSPRESFLAF-AKQHKGRISKKDFRKVLREHEIHLDEDQFKRLTEKLGF---TKEELSYLDFVSLFEDPTGNGPGAALDT----GSNHHVNDTKF---HYMSAEDCLAQLRDKLQQGYGDLYSAFYKIDSNRDGLVTMHDFRHLLDSFMFIIKPQEFQRLLGLLGLNLSSTLNYKEFLHLFQKPQSPV-YPPWLQSSH--SPKQSMGGEDLACEQAHYYLVTKAQGRWHDMAKTFCEIDSEGNGIVQKKDLRNVLFRFYLPIHPKEFEKLWGRYDPEGKGFLTHNEFLEKLGI--SFASGD-SGPSKQITEDNRACLEGHYNNQ-LKIQEEMD-----AFHKHQTKALDMKDIEQQIKDKFRDYYHDFSAAFAKIDRNK--DGQISVHDLRAMLQSLNFHLDDEQFANLLYRLRIKVTDNKLSYPDFLRMID-----DGRASKYGQRQRPVSAAETPPKLSLQ--------KVLLKLKEAVVSSHDALYKAFSSFDRDGTGTIKASELHQILDSFCFKLSDRQFGYLLSKLPL------GEEQTIDWKAFLHHFSTSHPEPSSSWVERVQHATRPRSTQGLTMKDILNHIREVVTARFYTVAQDFISLDYANINAVSKEDFRELFNKDFMLLTDEQFENLWNTLPLNPYGNLKYHEFL 1241 HSP 2 Score: 185.267 bits (469), Expect = 2.453e-46 Identity = 203/1033 (19.65%), Postives = 427/1033 (41.34%), Query Frame = 3
Query: 675 ILQMQISRNPNGIMKAFKLFDFNSDYCIQRHRLKKVMENFCCPLTEQQFDKLVARFSQSQNGNVNYMEFMKGMGIPIDNQGTIQTKNSEETINIDTR-----NTKGSRIFEAALIENGLLGSNELEQLLVIFRKKIEDQFSVLRKTFLVMDPNAEGFVTLEILYKILNQFAFPMSLNVFNQIMDKCAFKATGKIIWLSFLQKFQDPQSKHLNGSTIAIKSDHKCNPIQGVNSKPNIDDLLELLSNHISNNYSSFRDAFLQINQTHKGKIPKSEFLKILNRFQFYISADQLSIILKFLDPHNEGYISYHHFLQLFEQKENLSAHKWLNSNHKVVEPRKDLQLSSETIKSTISDNIGNKWSNVVHEFNLVDLNRTGYISVEDLKKIFKKLFGYLKDDDIDRLVAPLTLKDKKGRINYLELFNLLNVDTSPSDLEGLSTQIAESNDAQLKERHENHIEKMESIEKLGEARLNLMSPEELIVRIKDRIVQNRLNYHELYKKYECNDKKGGINKSQLRKMLTQFGYTLTNEQFSKISEILNFPTSSKSRIDFNGFLAIFADNRESSNDLMTDVCFLPKSNHHVNDIRGDDN-RNMNCQEVEDLLRQKIVNNFPDLMIAFHKFDRSNKGYLVKSDFQNFLLGNLIMIENDQFDLLCEKLGVGEKSKLTYNDFLNRFQIRDDMVDGHKWLDSKHRFNDPSTNAVPDMSAEDVYMLLPEKIYQYFHEISPTFIALVTSGTGFIRFRNLQHLLSRLQISMSKSEMKKLWKRYDTNNRNKLDYHDFLYNLGLLEKFGCGDLM--------GPSAAIIQNSHNALVDH------------------LVNQETKHQQSVDNQAVLGTHLHVEHLFEMVKRHLRVAYSDSALAFKAVANKKTLSGFFTINDLNKLLSQLHMFVTNDKFVQLAARMGFEGPDDSLSYEKFCCIFQD-SLNIDGQEKTVGSIRINQDLENPSKIGIEEAGNLNSTQIASIIGKRIVDQYSEIEKTFLVMDPENSGYVSMNEFRNLLVGFCLSLTDKQF 3674
IL +I N KA K D + + + K+++++FC PL+++ ++ L +++ NG ++YM+F++ +G+ G + ++E R + +R+ E L+ +E+++ + + Q R++FL +G ++ + K+L + + + F ++ +K F ++ +L F+ F+DP + + G+ + S+H N + + +D L L + + Y AF +I+ G + +F +L+ F F I + +L L + ++Y FL LF++ ++ WL S+H + L+ E + +W ++ F +D G + +DL+ + + + + + ++L + KG + + E L + + D G S QI E N A L+ + N ++ E ++ + + + +++ +IKD+ ++ + K + N K G I+ LR ML + L +EQF+ + L + +++ + FL + D R S K + + ++ Q+V L++ +V++ L AF FDR G + S+ L + + QF L KL +GE+ + + FL+ F W++ P + ++ +D+ + E + F+ ++ FI+L + + + + L ++ + ++ + + LW N L YH+F L +FG GD + G + I +AL + ++N+ Q ++ E + ++ L+ + + A + ++T G T ND + +L Q + ++ +L + + SY F F++ L EK+ Q P G + LN+ I +++D + + + FL D +G++S+ EF+ +L L+L++ +F
Sbjct: 488 ILCEKIEHNWADFSKAMKSCDSAGCGMVDKDQFKRILQSFCPPLSDRHYNMLCEQYNDGANGYISYMDFLRNVGVCTSAAGDLNGVSTEIVHGSQLREELRQDDLSARMKEIESQATQLVKKMPVEEVIGKLKDCVIGQEFSPRESFLAFAKQHKGRISKKDFRKVLREHEIHLDEDQFKRLTEKLGFTKE-ELSYLDFVSLFEDP-TGNGPGAALDTGSNHHVNDTKF--HYMSAEDCLAQLRDKLQQGYGDLYSAFYKIDSNRDGLVTMHDFRHLLDSFMFIIKPQEFQRLLGLLGLNLSSTLNYKEFLHLFQKPQSPVYPPWLQSSHSPKQSMGGEDLACEQAHYYLVTKAQGRWHDMAKTFCEIDSEGNGIVQKKDLRNVLFRFYLPIHPKEFEKLWGRYD-PEGKGFLTHNEFLEKLGISFASGD-SGPSKQITEDNRACLEGHYNNQLKIQEEMDAFHKHQTKALDMKDIEQQIKDKFRDYYHDFSAAFAKIDRN-KDGQISVHDLRAMLQSLNFHLDDEQFANLLYRLRIKVTD-NKLSYPDFLRMIDDGRAS------------KYGQRQRPVSAAETPPKLSLQKVLLKLKEAVVSSHDALYKAFSSFDRDGTGTIKASELHQILDSFCFKLSDRQFGYLLSKLPLGEEQTIDWKAFLHHFSTSHPEPSS-SWVERVQHATRPRSTQ--GLTMKDILNHIREVVTARFYTVAQDFISLDYANINAVSKEDFRELFNKDFMLLTDEQFENLWNTLPLNPYGNLKYHEF------LSQFGGGDALVSPYEKTAGDQSEFIYKPASALAGNHTPPESQAGIRRPKTAPAILNKPKTDTQRPKTAGASSRLVNCEQIEARLRGSLQKVWQEVLRACRDYDPERT--GEITTNDFSGVLKQFNAETNEEELERLTVKYDSRN-NGKFSYADF---FRNIMLGPKPLEKSPLHRMKVQKTRVPISTGPDGPCFLNAMM---RIQPKVLDCWRPMRRAFLSFDEARTGFISVQEFKQVLRKSGLNLSEDEF 1482 HSP 3 Score: 86.6557 bits (213), Expect = 3.124e-16 Identity = 111/552 (20.11%), Postives = 223/552 (40.40%), Query Frame = 3
Query: 2436 EVEDLLRQKIVNNFPDLMIAFHKFDRSNKGYLVKSDFQNFLLGNLIMIENDQFDLLCEKLGVGEKSKLTYNDFLNRFQIRDDMVDGHKWLDSKHRFNDPSTNAVPDMSAEDVYMLLPEKIYQYFHEISPTFIALVTSGTGFIRFRNLQHLLSRLQISMSKSEMKKLWKRYDTNNRNKLDYHDFLYNLGLLEKFGCGDLMGPSAAIIQNSHNALVDHLVNQETKHQQSVDNQAVL----GTHLHVEHLFEMVKRHLRVAYSDSALAFKAVANKKTLSGFFTINDLNKLLSQLHMFVTNDKFVQLAARMGFEGPDDSLSYEKFCCIFQDSLNI-DGQEKTVGSIRINQDLENPSKIGIEEAGNLNSTQIASIIGKRIVDQYSEIEKTFLVMDPENSGYVSMNEFRNLLVGFCLSLTDKQFNKYRNSLSTYIVKSANTDDKIDYRYFMKGFNQK---LNNLDIQXXXXXXXXXXXXXTYQQIQEHINESIKVRPKLFLNSFKSKDYASLNFISKSDFREIIERFLFTVPDSHFEKFWSNLKKNEFDNVNYGEFVK 4067
E+E ++ +++ N D+ AF D + + +F L ++ + QF+ L EK+ V + Y DFL +F + + K ++ N V +S ++ + L + + + + + G I+ + L+ +L M +E +KLW RY +N LDY + L NLG+ ++ + +N +AL V +E + +++ L ++ + +++ + +Y + AF+A + SG+ + L +++ + N F +L R+ + D + +E+F FQ ++ +GQ T+ ++ N + NP + E+ ++S I + + D Y +++ FLV+D G +S E R ++ +TD QF + I+ I Y F+ F K + N+ + E I+ F + KS D A + K F+ I++ F + D H+ ++Y +F++
Sbjct: 58 EIEGIIYRRVNENEEDVTNAFLTLDTEQNFTVTRGEFYRVLQNFILPLTESQFNELLEKIPVNNNGTIPYLDFLAKFLRTSSSATCARSV--KRSWSCSQANQV--LSLNELEIRLKNTVSKNLKNVVRSCRLFDYNQNGQIQKQELRRILENCCFKMKTAEFEKLWNRYCIGKKNTLDYRELLRNLGV-------NVELHNKPTAENVASALNREAVQREQQKEKNCRPPPPLKELSAEDYTIDDIEAALRKKISASYPNLVKAFRAFDG--SHSGYVSAEALQSVINNFIFPLPNGTFQKLMNRLAIKC-TDRIGWEQFLRRFQGPESMANGQ--TI-PMKPNHRV-NPVR---GESPVVSSEHILQKLQRHFQDAYPSLKQAFLVLDEGRKGRISRKELRRIVDCMMFRVTDAQFKEL------MIILDPQHTGFISYLQFLDLFEDKESVMGNI------------------------LCEKIEHNWADFSKAMKSCDSAGCGMVDKDQFKRILQSFCPPLSDRHYNMLCEQYNDGANGYISYMDFLR 558 HSP 4 Score: 76.6406 bits (187), Expect = 3.304e-13 Identity = 48/202 (23.76%), Postives = 93/202 (46.04%), Query Frame = 3
Query: 4182 ESIRKNLERLLNRNWQSIFNKCKAIDQAINECQTAEGSIPIDDFVYILSEYDDSLSAENLAAINL-YKTCKNGRFIKYGDLIRSIVLSSRGSLISNENVTEVQKKFLNVGIKTTNSDNSKDYFPQLFKLRRQILENWKDLRKNFLQSDKKQNGAVSGNIFMTIMKTVCGQLSADLFYHLMKIYDQDGTGNFHYKRFIEDFLK 4784
E I L L + WQ + C+ D G I +DF +L +++ + E L + + Y + NG+F Y D R+I+L + S + +VQK + + + + + +++ ++L+ W+ +R+ FL D+ + G +S F +++ LS D F+H++ +D+D Y F+ FL+
Sbjct: 1318 EQIEARLRGSLQKVWQEVLRACRDYDPE------RTGEITTNDFSGVLKQFNAETNEEELERLTVKYDSRNNGKF-SYADFFRNIMLGPKPLEKSPLHRMKVQK----TRVPISTGPDGPCFLNAMMRIQPKVLDCWRPMRRAFLSFDEARTGFISVQEFKQVLRKSGLNLSEDEFFHILGFFDKDLKAKVSYNDFLRAFLR 1508 HSP 5 Score: 73.559 bits (179), Expect = 2.889e-12 Identity = 156/834 (18.71%), Postives = 316/834 (37.89%), Query Frame = 3
Query: 642 YNAYTIDDIIPILQMQISRNPNGIMKAFKLFDFNSDYCIQRHRLKKVMENFCCPLTEQQFDKLVARFSQSQNGNVNYMEFMKGMGIPIDNQGTIQTKNSEETINIDTRNTKGSRIFEAALIENGLLGSNEL--EQLLVIFRKKIEDQFSVLRKTFLVMDPNAEGFVTLEILYKILNQFAFPMSLNVFNQIMDKCAFKATGKIIWLSFLQKFQDPQSKHLNGSTIAIKSDHKCNPIQGVNSKPNIDDLLELLSNH-----------------ISNNYSSFRDAFLQINQTHKGKIPKSEFLKILNRFQFYISADQLSIILKFLDPH-NEGYISYHHFLQLFEQKENLSAHKWLNSNHKVVEPRKDLQLSSETIKSTISDNIGNKWSNVVHEFNLVDLNRTGYISVEDLKKIFKKLFGYLKDDDIDRLVA--PLTLKDKKGRINYLELFNLLNVDTSPSDLEGL--STQIAESNDAQLKERHENHIEKMESIE---------KLGEARLNLMSPEELIVRI-KDRIVQNRLNYHELYKKYECNDKKGGINKSQLRKMLTQFG-----YTLTNEQFSKISEILNFPTSSKSRIDFNGFLAIFADNRESSNDLM---TDVCFL--PKSNHHVNDIRGDDNRNMNCQEVEDLLRQKIVNNFPDLMIAFHKFDRSNKGYLVKSDFQNFLLGNLIMIENDQFDLLCEKLGVGEKSKLTYNDFLNRFQIRDDMVDGHKWLDSK--HRFNDPSTNAVPDMSAED------VYMLLPEKIYQYFHEISPTFIALVTSGTGFIRFRNLQHLLSRLQISMSKSEMKKLWKRYDTNNRNKLDYHDFL 2987
++ + +D + L+ ++ + + AF D N D + H + ++++F + Q+F +L+ + + +NY EF+ P Q + +++ +G +L EQ K + ++ + KTF +D G V + L +L +F P+ F ++ + + G + FL+K + +G + I D++ N++ I + ++ H + Y F AF +I++ G+I + +L F++ +Q + +L L + +SY FL++ + A K+ V +LS + + + + + + + F+ D + TG I +L +I L D L++ PL + +L F+ + + S S +E + +T+ + +K+ NHI ++ + L A +N +S E+ KD ++ + L+ N + + L+QFG + + SE + P S+ LA ES + T L PK++ G +R +NC+++E LR + + +++ A +D G + +DF L ++ + L K K +Y DF + G K L+ HR T VP + D M + K+ + + F++ + TGFI + + +L + +++S+ E + +D + + K+ Y+DFL
Sbjct: 714 FHYMSAEDCLAQLRDKLQQGYGDLYSAFYKIDSNRDGLVTMHDFRHLLDSFMFIIKPQEFQRLLGLLGLNLSSTLNYKEFLHLFQKP---QSPVYPP-----------------WLQSSHSPKQSMGGEDLACEQAHYYLVTKAQGRWHDMAKTFCEIDSEGNGIVQKKDLRNVLFRFYLPIHPKEFEKLWGRYDPEGKGFLTHNEFLEKLGISFASGDSGPSKQITEDNRACLEGHYNNQLKIQEEMDAFHKHQTKALDMKDIEQQIKDKFRDYYHDFSAAFAKIDRNKDGQISVHDLRAMLQSLNFHLDDEQFANLLYRLRIKVTDNKLSYPDFLRMIDDGR---ASKYGQRQRPVSAAETPPKLSLQKVLLKLKEAVVSSHDALYKAFSSFDRDGTGTIKASELHQILDSFCFKLSDRQFGYLLSKLPLGEEQTIDWKAFLHHFSTSHPEPSSSWVERVQHATRPRSTQGLTMKDIL-NHIREVVTARFYTVAQDFISLDYANINAVSKEDFRELFNKDFMLLTDEQFENLWNTLPLNP----YGNLKYHEFLSQFGGGDALVSPYEKTAGDQSEFIYKPASA---------LAGNHTPPESQAGIRRPKTAPAILNKPKTDTQRPKTAGASSRLVNCEQIEARLRGSLQKVWQEVLRACRDYDPERTGEITTNDFSGVLKQFNAETNEEELERLTVKYDSRNNGKFSYADFFRNIML------GPKPLEKSPLHRMKVQKTR-VPISTGPDGPCFLNAMMRIQPKVLDCWRPMRRAFLSFDEARTGFISVQEFKQVLRKSGLNLSEDEFFHILGFFDKDLKAKVSYNDFL 1503 HSP 6 Score: 52.7582 bits (125), Expect = 7.002e-6 Identity = 94/459 (20.48%), Postives = 173/459 (37.69%), Query Frame = 3
Query: 3504 LNSTQIASIIGKRIVDQYSEIEKTFLVMDPENSGYVSMNEFRNLLVGFCLSLTDKQFNKYRNSLSTYIVKSANTDDKIDYRYFMKGFNQKLNNLDIQXXXXXX---XXXXXXXTYQQIQEHINESIKVRPKLFLNSFKSKDYASLNFISKSDFREIIERFLFTVPDSHFEKFWSNLKKNEFDNVNYGEFVKMXXXXXXXXXXXXXXXXXXXXXXXXXXNNIETDEMDTDESIRKNLERLLNRNWQSIFNKCK---------AIDQAINECQTA-----EGSIPIDDFVYILSEYDDS----LSAENLAAI--NLYKTCKNGRF--------IKYGDLIRSIVLSSR---GSLISNENVTEVQKKFLNVGIKTTNSDNSKDYFPQLFKLRRQILENWKDLRKNFLQSDKKQNGAVSGNIFMTIMKTVCGQLSADLFYHLMKIYDQDGTGNFHYKRFIEDF 4778
L +I II +R+ + ++ FL +D E + V+ EF +L F L LT+ QFN+ + N + I Y F+ F + ++ + ++ +++ + ++ K + S + DY I K + R I+E F + + FEK W+ + + ++Y E ++ + N+E T E++ L R + Q C+ A D I++ + A S P + V +D S +SAE L ++ N NG F IK D I R ++N ++ ++ + S ++ Q KL+R + + L++ FL D+ + G +S I+ + +++ F LM I D TG Y +F++ F
Sbjct: 54 LTPLEIEGIIYRRVNENEEDVTNAFLTLDTEQNFTVTRGEFYRVLQNFILPLTESQFNELLEKIPV------NNNGTIPYLDFLAKFLRTSSSATCARSVKRSWSCSQANQVLSLNELEIRLKNTVSKNLKNVVRSCRLFDYNQNGQIQKQELRRILENCCFKMKTAEFEKLWNRYCIGKKNTLDYRELLRNLGV------------------------NVELHNKPTAENVASALNREAVQREQQKEKNCRPPPPLKELSAEDYTIDDIEAALRKKISASYP--NLVKAFRAFDGSHSGYVSAEALQSVINNFIFPLPNGTFQKLMNRLAIKCTDRIGWEQFLRRFQGPESMANGQTIPMKPNHRVNPVRGESPVVSSEHILQ--KLQRHFQDAYPSLKQAFLVLDEGRKGRISRKELRRIVDCMMFRVTDAQFKELMIILDPQHTGFISYLQFLDLF 478
BLAST of EF-hand calcium-binding domain-containing protein 6 vs. Ensembl Xenopus
Match: immp2l (inner mitochondrial membrane peptidase subunit 2 [Source:Xenbase;Acc:XB-GENE-1010851]) HSP 1 Score: 339.732 bits (870), Expect = 7.694e-95 Identity = 243/941 (25.82%), Postives = 448/941 (47.61%), Query Frame = 3
Query: 180 DPKLQISEINPIVKSQLLKNHKEILKALQTFDIECSGLVTKGELRRVLDSYCLPMTTDQYEQFLSHNSINYSKGSINYKELIDKIYDKTSAVGHFKKNIKLPQLQRSATTICLQNKHFTLPTTRRSSGKISRSQLFXXXXXXXXXXXXXXXXWNYNAYTIDDIIPILQMQISRNPNGIMKAFKLFDFNSDYCIQRHRLKKVMENFCCPLTEQQFDKLVARFSQSQNGNVNYMEFMKGMGIPIDNQGTIQTKNSEETIN---IDTRNTKGSRIFEAALIENGLLGSNELEQLLVIFRKKIEDQFSVLRKTFLVMDPNAEGFVTLEILYKILNQFAFPMSLNVFNQIMDKCAFKATGKIIWLSFLQKFQDPQSKHLNGSTIAIKSDHKCNPIQGVNSKPNIDDLLELLSNHISNNYSSFRDAFLQINQTHKGKIPKSEFLKILNRFQFYISADQLSIILKFLDPHNEGYISYHHFLQLFEQKENLSAHKWLNSNHKVVEPRKD--LQLSSETIKSTISDNIGNKWSNVVHEFNLVDLNRTGYISVEDLKKIFKKLFGYLKDDDIDRLVAPLTLKDKKGRINYLELFNLLNVDTSPS-DLEGLSTQIAESNDAQLKERHENHIEKMESIEKLGEARLNLMSPEELIVRIKDRIVQNRLNYHELYKKYECNDKKGGINKSQLRKMLTQFGYTLTNEQFSKISEILNFPTSSKSRIDFNGFLAIFADNRESSNDLMTDVCFLPKSNHHVNDIRGDDNRNMNCQEVEDLLRQKIVNNFPDLMIAFHKFDRSNKGYLVKSDFQNFLLGNLIMIENDQFDLLCEKLGVGEKSKLTYNDFLNRFQIRDDMVDGHKWLDSKHRFNDPSTNAVPDMSAEDVYMLLPEKIYQYFHEISPTFIALVTSGTGFIRFRNLQHLLSRLQISMSKSEMKKLWKRYDTNNRNKLDYHDF 2984
DP L EI I+ ++ +N +++ A T D E + VT+GE RVL ++ LP+T Q+ + L +N + G+I Y + + K L+ S++ C + + +RS +Q+ +++++ L+ +S+N ++++ +LFD+N + IQ+ L++++EN C + +F+KL R+ + ++Y E ++ +G+ ++ +N +N + K ++ ++ + RKKI + L K F D + G+V+ E L ++N F FP+ F ++M++ A K T +I W FL++FQ P+S NG TI +K +H+ NP++G + + + +L+ L H + Y S + AFL +++ KG+I + E +I++ F ++ Q ++ LDP + G+ISY FL LFE KE++S HKWL H + P K+ + L E + + + + I + W++ D G + + K+I + L D + L G I+Y++ + V TS + DL G+ST+I + + + R ++ +M+ IE + M EE+I ++KD ++ + E + + KG I+K RK+L + L +QF +++E L F +K + + F+++F D + D SNHHVND + M+ ++ LR K+ + DL AF+K D + G + DF++ L + +I+ +F L LG+ S L Y +FL+ FQ V WL S H + + D++ E + L K +H+++ TF + + G G ++ ++L+++L R + + E +KLW R ++ + YHDF
Sbjct: 51 DPTLTPLEIEGIIYRRVNENEEDVTNAFLTLDTEQNFTVTRGEFYRVLQNFILPLTESQFNELLEKIPVN-NNGTIPYLDFLAKF------------------LRTSSSATCAR-------SVKRSWSCSQANQVL----------------------SLNELEIRLKNTVSKNLKNVVRSCRLFDYNQNGQIQKQELRRILENCCFKMKTAEFEKLWNRYCIGKKNTLDYRELLRNLGVNVELHNKPTAENVASALNREAVQREQQKEKNCRPPPPLKELSAEDYTIDDIEAALRKKISASYPNLVKAFRAFDGSHSGYVSAEALQSVINNFIFPLPNGTFQKLMNRLAIKCTDRIGWEQFLRRFQGPESM-ANGQTIPMKPNHRVNPVRGESPVVSSEHILQKLQRHFQDAYPSLKQAFLVLDEGRKGRISRKELRRIVDCMMFRVTDAQFKELMIILDPQHTGFISYLQFLDLFEDKESVSGHKWL---HNMRTPLKETPVTLEWEKMGNILCEKIEHNWADFSKAMKSCDSAGCGMVDKDQFKRILQSFCPPLSDRHYNMLCEQYN-DGANGYISYMDFLRNVGVCTSAAGDLNGVSTEIVHGSQLREELRQDDLSARMKEIESQATQLVKKMPVEEVIGKLKDCVIGQEFSPRESFLAF-AKQHKGRISKKDFRKVLREHEIHLDEDQFKRLTEKLGF---TKEELSYLDFVSLFEDPTGNGPGAALDT----GSNHHVNDTKF---HYMSAEDCLAQLRDKLQQGYGDLYSAFYKIDSNRDGLVTMHDFRHLLDSFMFIIKPQEFQRLLGLLGLNLSSTLNYKEFLHLFQKPQSPV-YPPWLQSSH--SPKQSMGGEDLACEQAHYYLVTKAQGRWHDMAKTFCEIDSEGNGIVQKKDLRNVLFRFYLPIHPKEFEKLWGR----DKFRDYYHDF 920 HSP 2 Score: 198.364 bits (503), Expect = 2.426e-50 Identity = 260/1209 (21.51%), Postives = 488/1209 (40.36%), Query Frame = 3
Query: 663 DIIPILQMQISRNPNGIMKAFKLFDFNSDYCIQRHRLKKVMENFCCPLTEQQFDKLVARFSQSQNGNVNYMEFMKGMGIPIDNQGTIQTKNSEETINIDTRNTKGSRIFEAALIENGLLGSNELEQLLVIFRKKIEDQFSVLRKTFLVMDPNAEGFVTLEILYKILNQFAFPMSLNVFNQIMDKCAFKATGKIIWLSFLQKF-------QDPQSKHLNGS--TIAIKSDHK----CNPIQGVNSKP----NIDDLLELLSNHISNNYSSFRDAFLQINQTHKGKIPKSEFLKILNRFQFYISADQLSIILKFLDPHNEGYISYHHFLQLFEQKENLSAHKW--LNSNHKVVEPRKDLQL-SSETIKSTISDNIGNKWSNVVHEFNLVDLNRTGYISVEDLKKIFKKLFGYLKDDDIDRLVAPLTLKDKKGRINYLELFNLLNVDTSPSD---LEGLSTQIAESNDAQLKERHENHIEKMESIEKLGEARLNLMSPEELIVRIKDRIVQNRLNYHELYKKYECNDKKGG-INKSQLRKMLTQFGYTLTNEQFSKISEILNFPTSSKSRIDFNGFLAIFADNRESSNDLMTDVCFLPKSNHHVNDIRGDD-------NRNMNCQEVEDLLRQKIVNNFPDLMI--------AFHKFDRSNKGYLVKSDFQNFLLGNLIMIENDQFDLLCEKLGVGEKSKLTYNDFLNRFQIRDDMVDGH-KWLD--SKHRFNDPSTNAVPDMSAEDVYMLLPEKIYQYFHEISPTFIALVTSGTGFIRFRNLQHLLSRLQISMSKSEMKKLWKRYDTNNRNKLDYHDFL----------YNLGLLEKFGCGDLMGPSAAIIQNSHNALVD------HLVNQETKHQQSVDNQAVLGTHL-----------HVEHLFEMVKR-HLRVAYSDSALAFKAVANKKTLSGFFTINDLNKLLSQLHMFVTNDKFVQLAARMGFEGPDDSLSYEKFCCIFQDSLNIDGQEKTVGSI-RINQDLENPSKIGIEEAGNLNSTQIASIIGKRIVDQYSEIEKTFLVMDPENSGYVSMNEFRNLLVGFCLSLTDKQFNKYRNSLSTYIVKSANTDDKIDYRYFMKGFN----QKLNNLDIQXXXXXXXXXXXXXTYQQIQEHINESIKVRPKLFLNSFKSKDYASLNFISKSDFREIIERFLFTVPDSHFEKFWSNLKKNEFDNVNYGEFV 4064
+I I+ +++ N + AF D ++ + R +V++NF PLTE QF++L+ + + NG + Y++F+ ++T +S R+ S+ N +L NELE + + + + ++ + D N G + + L +IL F M F ++ ++ + + L+ P ++++ + A++ + + C P + IDD+ L IS +Y + AF + +H G + ++N F F + ++ L I + FL+ F+ E+++ + + NH+V R + + SSE I + + + + ++ F ++D R G IS ++L++I + + D L+ L + G I+YL+ +L S S L + T + E+ EKM +I + ++I N ++ + K C+ G ++K Q +++L F L++ ++ + E N + I + FL ++ DL + + ++R DD + Q V+ + ++++ D +I +F F + +KG + K DF+ L + I ++ DQF L EKLG K +L+Y DF++ F+ D +G LD S H ND + MSAED L +K+ Q + ++ F + ++ G + + +HLL + E ++L N + L+Y +FL Y L MG + +H LV H + + S N V L H + ++ R R Y D + AF + K G +++DL +L L+ + +++F L R+ + D+ LSY F + D G+ G R E P K+ ++ ++ + + +V + + K F D + +G + +E +L FC L+D+QF + L + ID++ F+ F+ + ++ + A + +T + I HI E + R F S DYA++N +SK DFRE+ + + D FE W+ L N + N+ Y EF+
Sbjct: 58 EIEGIIYRRVNENEEDVTNAFLTLDTEQNFTVTRGEFYRVLQNFILPLTESQFNELLEKIPVNNNGTIPYLDFLAKF---------LRTSSSATCARSVKRSWSCSQA-------NQVLSLNELE---IRLKNTVSKNLKNVVRSCRLFDYNQNGQIQKQELRRILENCCFKMKTAEFEKLWNRYCIGKKNTLDYRELLRNLGVNVELHNKPTAENVASALNREAVQREQQKEKNCRPPPPLKELSAEDYTIDDIEAALRKKISASYPNLVKAFRAFDGSHSGYVSAEALQSVINNFIFPLPNGTFQKLMNRLAIKCTDRIGWEQFLRRFQGPESMANGQTIPMKPNHRVNPVRGESPVVSSEHILQKLQRHFQDAYPSLKQAFLVLDEGRKGRISRKELRRIVDCMMFRVTDAQFKELMIILDPQ-HTGFISYLQFLDLFEDKESVSGHKWLHNMRTPLKETPVTL-------EWEKMGNI-------------------LCEKIEHNWADFSKAMKS--CDSAGCGMVDKDQFKRILQSFCPPLSDRHYNMLCEQYN--DGANGYISYMDFLRNVGVCTSAAGDLNGVSTEIVHGSQLREELRQDDLSARMKEIESQATQLVKKMPVEEVIGKLKDCVIGQEFSPRESFLAFAKQHKGRISKKDFRKVLREHEIHLDEDQFKRLTEKLGF-TKEELSYLDFVSLFE--DPTGNGPGAALDTGSNHHVNDTKFHY---MSAEDCLAQLRDKLQQGYGDLYSAFYKIDSNRDGLVTMHDFRHLLDSFMFIIKPQEFQRLLGLLGLNLSSTLNYKEFLHLFQKPQSPVYPPWLQSSHSPKQSMGGEDLACEQAHYYLVTKAQGRWHDMAKTFCEIDSEGNGIVQKKDLRNVLFRFYLPIHPKEFEKLWGRDKFRDYYHDFSAAFAKIDRNK--DGQISVHDLRAMLQSLNFHLDDEQFANLLYRLRIKVTDNKLSYPDFLRMIDD-----GRASKYGQRQRPVSAAETPPKLSLQ--------KVLLKLKEAVVSSHDALYKAFSSFDRDGTGTIKASELHQILDSFCFKLSDRQFGYLLSKLPL------GEEQTIDWKAFLHHFSTSHPEPSSSWVERVQHATRPRSTQGLTMKDILNHIREVVTARFYTVAQDFISLDYANINAVSKEDFRELFNKDFMLLTDEQFENLWNTLPLNPYGNLKYHEFL 1189 HSP 3 Score: 145.591 bits (366), Expect = 3.576e-34 Identity = 216/1175 (18.38%), Postives = 459/1175 (39.06%), Query Frame = 3
Query: 165 ANYKMDPKLQISEINPIVKS----QLLKNH-----KEILKALQTFDIECSGLVTKGELRRVLDSYCLPMTTDQYEQFLSHNSINYSKGSINYKELIDKIYDKTSAVGH-FKKNIKLPQLQRSATTICLQNKHFTLPTTRRSSGKISRSQLFXXXXXXXXXXXXXXXXWNYNAYTIDDIIPILQMQISRNPNGIMKAFKLFDFNSDYCIQRHRLKKVMENFCCPLTEQQFDKLVARFSQSQNGNVNYMEFMKGMGIPIDNQGTIQTKNSEETINIDTR-----NTKGSRIFEAALIENGLLGSNELEQLLVIFRKKIEDQFSVLRKTFLVMDPNAEGFVTLEILYKILNQFAFPMSLNVFNQIMDKCAFKATGKIIWLSFLQKFQDPQSKHLNGSTIAIKSDHKCNPIQGVNSKPNIDDLLELLSNHISNNYSSFRDAFLQINQTHKGKIPKSEFLKILNRFQFYISADQLSIILKFLDPHNEGYISYHHFLQLFEQKENLSAHKWLNSNHKVVEPRKDLQLSSETIKSTISDNIGNKWSNVV------------------------------------------------HEFNL----VDLNRTGYISVEDLKKIFKKLFGYLKDDDIDRLVAPLTLKDKKGRINYLELFNLLNVDTSPSDLEGLSTQIAESNDAQLKERHENHIEKMESIEKLGEARLNLMSPEELIVRIKDRIVQNRLNYHELYKKYECNDK--KGGINKSQLRKMLTQFGYTLTNEQFSKISEILNFPTSSKSRIDFNGFLAIFADNR-ESSNDLMTDVCFLPKSNHHVNDIRGDDNRNMNCQEVEDLLRQKIVNNFPDLMIAFHKFDRSNKGYLVKSDFQNFLLGNLIMIENDQFDLLCEKLGVGEKSKLTYNDFLNRFQIRDDMVDGHK---------------WLDSKHR-----------------FNDPSTNAVPDMSA---------EDVYMLLPEKIYQYFHEISPTFIALVTSGTGFIRFRNLQHLLSRLQISMSKSEMKKLWKRYDTNNRNKLDYHDFLYNLGLLEKFGCGDLMGPSAAIIQNSHNALVDHLVNQETKHQQSV--DNQAVLGTHLHVEHLFEMVKRHLRVAYSDSALAFKAVANKKTLSGFFTINDLNKLLSQLHMFVTNDKFVQLAARMGF 3350
N++++P + +P+V S Q L+ H + +A D G +++ ELRR++D +T Q+++ + ++ G I+Y + +D DK S GH + N++ P L+ + T+ W + + IL +I N KA K D + + + K+++++FC PL+++ ++ L +++ NG ++YM+F++ +G+ G + ++E R + +R+ E L+ +E+++ + + Q R++FL +G ++ + K+L + + + F ++ +K F ++ +L F+ F+DP + + G+ + S+H N + + +D L L + + Y AF +I+ G + +F +L+ F F I + +L L + ++Y FL LF++ ++ WL S+H + L+ E + +W ++ H+F+ +D N+ G ISV DL+ + + L +L D+ L+ L +K +++Y + +++ +G +++ + + + E+ KL S +++++++K+ +V ++ LYK + D+ G I S+L ++L F + L++ QF + + P + ID+ FL F+ + E S+ + V H R + + +++ + +R+ + F + F D +N + K DF+ + +++ ++QF+ L L + L Y++FL++F D +V ++ L H N P T+ +A E + L + + + E+ TG I + +L + ++ E+++L +YD+ N K Y DF N+ ++GP H V Q+T+ S D L + ++ R +R A+ L+F + +GF ++ + ++L + + ++ D+F + +GF
Sbjct: 384 PNHRVNP---VRGESPVVSSEHILQKLQRHFQDAYPSLKQAFLVLDEGRKGRISRKELRRIVDCMMFRVTDAQFKELMIILDPQHT-GFISYLQFLDLFEDKESVSGHKWLHNMRTP-LKETPVTL----------------------------------------EW-------EKMGNILCEKIEHNWADFSKAMKSCDSAGCGMVDKDQFKRILQSFCPPLSDRHYNMLCEQYNDGANGYISYMDFLRNVGVCTSAAGDLNGVSTEIVHGSQLREELRQDDLSARMKEIESQATQLVKKMPVEEVIGKLKDCVIGQEFSPRESFLAFAKQHKGRISKKDFRKVLREHEIHLDEDQFKRLTEKLGFTKE-ELSYLDFVSLFEDP-TGNGPGAALDTGSNHHVNDTKF--HYMSAEDCLAQLRDKLQQGYGDLYSAFYKIDSNRDGLVTMHDFRHLLDSFMFIIKPQEFQRLLGLLGLNLSSTLNYKEFLHLFQKPQSPVYPPWLQSSHSPKQSMGGEDLACEQAHYYLVTKAQGRWHDMAKTFCEIDSEGNGIVQKKDLRNVLFRFYLPIHPKEFEKLWGRDKFRDYYHDFSAAFAKIDRNKDGQISVHDLRAMLQSLNFHLDDEQFANLLYRLRIKVTDNKLSYPDFLRMID--------DGRASKYGQ---------RQRPVSAAETPPKL--------SLQKVLLKLKEAVVS---SHDALYKAFSSFDRDGTGTIKASELHQILDSFCFKLSDRQFGYL--LSKLPLGEEQTIDWKAFLHHFSTSHPEPSSSWVERV-------QHAT--RPRSTQGLTMKDILNHIREVVTARFYTVAQDFISLDYANINAVSKEDFRELFNKDFMLLTDEQFENLWNTLPLNPYGNLKYHEFLSQFGGGDALVSPYEKTAGDQSEFIYKPASALAGNHTPPESQAGIRRPKTAPAILNKPKTDTQRPKTAGASSRLVNCEQIEARLRGSLQKVWQEVLRACRDYDPERTGEITTNDFSGVLKQFNAETNEEELERLTVKYDSRNNGKFSYADFFRNI----------MLGPKPLEKSPLHRMKV-----QKTRVPISTGPDGPCFLNAMMRIQPKVLDCWRPMRRAF----LSF-----DEARTGFISVQEFKQVLRKSGLNLSEDEFFHI---LGF 1436 HSP 4 Score: 89.3521 bits (220), Expect = 4.426e-17 Identity = 111/552 (20.11%), Postives = 229/552 (41.49%), Query Frame = 3
Query: 2436 EVEDLLRQKIVNNFPDLMIAFHKFDRSNKGYLVKSDFQNFLLGNLIMIENDQFDLLCEKLGVGEKSKLTYNDFLNRFQIRDDMVDGHKWLDSKHRFNDPSTNAVPDMSAEDVYMLLPEKIYQYFHEISPTFIALVTSGTGFIRFRNLQHLLSRLQISMSKSEMKKLWKRYDTNNRNKLDYHDFLYNLGLLEKFGCGDLMGPSAAIIQNSHNALVDHLVNQETKHQQSVDNQAVL----GTHLHVEHLFEMVKRHLRVAYSDSALAFKAVANKKTLSGFFTINDLNKLLSQLHMFVTNDKFVQLAARMGFEGPDDSLSYEKFCCIFQDSLNI-DGQEKTVGSIRINQDLENPSKIGIEEAGNLNSTQIASIIGKRIVDQYSEIEKTFLVMDPENSGYVSMNEFRNLLVGFCLSLTDKQFNKYRNSLSTYIVKSANTDDKIDYRYFMKGFNQK---LNNLDIQXXXXXXXXXXXXXTYQQIQEHINESIKVRPKLFLNSFKSKDYASLNFISKSDFREIIERFLFTVPDSHFEKFWSNLKKNEFDNVNYGEFVK 4067
E+E ++ +++ N D+ AF D + + +F L ++ + QF+ L EK+ V + Y DFL +F + + K ++ N V +S ++ + L + + + + + G I+ + L+ +L M +E +KLW RY +N LDY + L NLG+ ++ + +N +AL V +E + +++ L ++ + +++ + +Y + AF+A + SG+ + L +++ + N F +L R+ + D + +E+F FQ ++ +GQ T+ ++ N + NP + E+ ++S I + + D Y +++ FLV+D G +S E R ++ +TD QF + I+ I Y F+ F K + + +M + ++++ + E I+ F + KS D A + K F+ I++ F + D H+ ++Y +F++
Sbjct: 58 EIEGIIYRRVNENEEDVTNAFLTLDTEQNFTVTRGEFYRVLQNFILPLTESQFNELLEKIPVNNNGTIPYLDFLAKFLRTSSSATCARSV--KRSWSCSQANQV--LSLNELEIRLKNTVSKNLKNVVRSCRLFDYNQNGQIQKQELRRILENCCFKMKTAEFEKLWNRYCIGKKNTLDYRELLRNLGV-------NVELHNKPTAENVASALNREAVQREQQKEKNCRPPPPLKELSAEDYTIDDIEAALRKKISASYPNLVKAFRAFDG--SHSGYVSAEALQSVINNFIFPLPNGTFQKLMNRLAIKC-TDRIGWEQFLRRFQGPESMANGQ--TI-PMKPNHRV-NPVR---GESPVVSSEHILQKLQRHFQDAYPSLKQAFLVLDEGRKGRISRKELRRIVDCMMFRVTDAQFKEL------MIILDPQHTGFISYLQFLDLFEDKESVSGHKWLHNMRTPLKETPVTLEWEKMGNILCEKIEHNWADFSKAMKSCDSAGCGMVDKDQFKRILQSFCPPLSDRHYNMLCEQYNDGANGYISYMDFLR 582 HSP 5 Score: 80.1073 bits (196), Expect = 3.644e-14 Identity = 197/1015 (19.41%), Postives = 378/1015 (37.24%), Query Frame = 3
Query: 189 LQISEINPIVKSQLLKNHKEILKALQTFDIECSGLVTKGELRRVLDSYCLPMTTDQYEQFLSHNSINYSKGSINYKELIDKIYDKTSAVGHFKKNIKLPQLQRSATTICLQNKHFTLPTTRRSSGKISRSQLFXXXXXXXXXXXXXXXXWNYNAYTIDDIIPILQMQISRNPNGIMKAFKLFDFNSDYCIQRHRLKKVMENFCCPLTEQQFDKLVARFSQSQNGNVNYMEFMKGMGIPIDN-QGTIQTKNSEETINIDTRNTKGSRIFEAALIENGLLGSNELEQLLVIFRKKIEDQFSVLRKTFLVMDPNAEGFVTLEILYKILNQFAFPMSLNVFNQIMDKCAFKATGKIIWLSFLQKFQDPQS--------------KHLNGSTIAIKSDHK--CNPIQG-----------VNSKPN----IDDLLELL-----------------SNHISNNYSSFRDAFLQINQTHKGKIPKSEFLKILNRFQFYISADQLSIILKFLDPH-NEGYISYHHFLQLFEQKENLSAHKWLNSNHKVVEPRKDLQLSSETIKSTISDNIGNKWSNVVHEFNLVDLNRTGYISVEDLKKIFKKLFGYLKDDDIDRLVA--PLTLKDKKGRINYLELFNLLNVDTSPSDLEGL--STQIAESNDAQLKERHENHIEKMESIE---------KLGEARLNLMSPEELIVRI-KDRIVQNRLNYHELYKKYECNDKKGGINKSQLRKMLTQFG-----YTLTNEQFSKISEILNFPTSSKSRIDFNGFLAIFADNRESSNDLM---TDVCFL--PKSNHHVNDIRGDDNRNMNCQEVEDLLRQKIVNNFPDLMIAFHKFDRSNKGYLVKSDFQNFLLGNLIMIENDQFDLLCEKLGVGEKSKLTYNDFLNRFQIRDDMVDGHKWLDSK--HRFNDPSTNAVPDMSAED------VYMLLPEKIYQYFHEISPTFIALVTSGTGFIRFRNLQHLLSRLQISMSKSEMKKLWKRYDTNNRNKLDYHDFL 2987
L+ ++ I+ ++ N + KA+++ D G+V K + +R+L S+C P++ D++ L + + G I+Y + + + TSA G L +T I G R +L S + +S + ++++I L+ + ++F F I + +KV+ L E QF +L + ++ ++Y++F+ P N G S +N DT+ S E L R K++ + L F +D N +G VT+ +L+ F F + F +++ + + + FL FQ PQS + + G +A + H QG ++S+ N DL +L + + Y F AF +I++ G+I + +L F++ +Q + +L L + +SY FL++ + A K+ V +LS + + + + + + + F+ D + TG I +L +I L D L++ PL + +L F+ + + S S +E + +T+ + +K+ NHI ++ + L A +N +S E+ KD ++ + L+ N + + L+QFG + + SE + P S+ LA ES + T L PK++ G +R +NC+++E LR + + +++ A +D G + +DF L ++ + L K K +Y DF + G K L+ HR T VP + D M + K+ + + F++ + TGFI + + +L + +++S+ E + +D + + K+ Y+DFL
Sbjct: 504 LEWEKMGNILCEKIEHNWADFSKAMKSCDSAGCGMVDKDQFKRILQSFCPPLS-DRHYNMLCEQYNDGANGYISYMDFLRNVGVCTSAAG---------DLNGVSTEIV--------------HGSQLREELRQDDLSARMKEIESQATQLVKKMPVEEVIGKLKDCVIGQEFSPRESFLAFAKQHKGRISKKDFRKVLREHEIHLDEDQFKRLTEKLGFTKE-ELSYLDFVSLFEDPTGNGPGAALDTGSNHHVN-DTKFHYMS-----------------AEDCLAQLRDKLQQGYGDLYSAFYKIDSNRDGLVTMHDFRHLLDSFMFIIKPQEFQRLLGLLGLNLSSTLNYKEFLHLFQKPQSPVYPPWLQSSHSPKQSMGGEDLACEQAHYYLVTKAQGRWHDMAKTFCEIDSEGNGIVQKKDLRNVLFRFYLPIHPKEFEKLWGRDKFRDYYHDFSAAFAKIDRNKDGQISVHDLRAMLQSLNFHLDDEQFANLLYRLRIKVTDNKLSYPDFLRMIDDGR---ASKYGQRQRPVSAAETPPKLSLQKVLLKLKEAVVSSHDALYKAFSSFDRDGTGTIKASELHQILDSFCFKLSDRQFGYLLSKLPLGEEQTIDWKAFLHHFSTSHPEPSSSWVERVQHATRPRSTQGLTMKDIL-NHIREVVTARFYTVAQDFISLDYANINAVSKEDFRELFNKDFMLLTDEQFENLWNTLPLNP----YGNLKYHEFLSQFGGGDALVSPYEKTAGDQSEFIYKPASA---------LAGNHTPPESQAGIRRPKTAPAILNKPKTDTQRPKTAGASSRLVNCEQIEARLRGSLQKVWQEVLRACRDYDPERTGEITTNDFSGVLKQFNAETNEEELERLTVKYDSRNNGKFSYADFFRNIML------GPKPLEKSPLHRMKVQKTR-VPISTGPDGPCFLNAMMRIQPKVLDCWRPMRRAFLSFDEARTGFISVQEFKQVLRKSGLNLSEDEFFHILGFFDKDLKAKVSYNDFL 1451 HSP 6 Score: 77.0258 bits (188), Expect = 2.717e-13 Identity = 48/202 (23.76%), Postives = 93/202 (46.04%), Query Frame = 3
Query: 4182 ESIRKNLERLLNRNWQSIFNKCKAIDQAINECQTAEGSIPIDDFVYILSEYDDSLSAENLAAINL-YKTCKNGRFIKYGDLIRSIVLSSRGSLISNENVTEVQKKFLNVGIKTTNSDNSKDYFPQLFKLRRQILENWKDLRKNFLQSDKKQNGAVSGNIFMTIMKTVCGQLSADLFYHLMKIYDQDGTGNFHYKRFIEDFLK 4784
E I L L + WQ + C+ D G I +DF +L +++ + E L + + Y + NG+F Y D R+I+L + S + +VQK + + + + + +++ ++L+ W+ +R+ FL D+ + G +S F +++ LS D F+H++ +D+D Y F+ FL+
Sbjct: 1266 EQIEARLRGSLQKVWQEVLRACRDYDPE------RTGEITTNDFSGVLKQFNAETNEEELERLTVKYDSRNNGKF-SYADFFRNIMLGPKPLEKSPLHRMKVQK----TRVPISTGPDGPCFLNAMMRIQPKVLDCWRPMRRAFLSFDEARTGFISVQEFKQVLRKSGLNLSEDEFFHILGFFDKDLKAKVSYNDFLRAFLR 1456
BLAST of EF-hand calcium-binding domain-containing protein 6 vs. Ensembl Mouse
Match: Efcab6 (EF-hand calcium binding domain 6 [Source:MGI Symbol;Acc:MGI:1924877]) HSP 1 Score: 193.741 bits (491), Expect = 5.099e-49 Identity = 222/1143 (19.42%), Postives = 480/1143 (41.99%), Query Frame = 3
Query: 678 LQMQISRNPNGIMKAFKLFDFNSDYCIQRHRLKKVMENFCCPLTEQQFDKLVARFSQSQNGNVNYMEFMKGMGIPIDNQGTIQTKNSEETINIDTRNTKGSRIFEAALIENGLLGSNELEQLLVIFRKKIEDQFSVLRKTFLVMDPNAEGFVTLEILYKILNQFAFPMSLNVFNQIMDKCAFKATGKIIWLSFLQKFQDPQSKHLNGSTIAIKSDHKCNPIQGVNSKPNIDDLLELLSNHISNNYSSFRDAFLQINQTHKGKIPKSEFLKILNRFQFYISADQLSIILKFLDPHNEGYISYHHFLQLFEQKENLSAHKWLNSNHKVVEPRKDLQLSSETIKSTISDNIGNKWSNVVHEFNLVDLNRTGYISVEDLKKIFKKLFGYLKDDDIDRLVAPLTLKDKKGRINYLELFNLLNVDTSPSDLEGLSTQIAESNDAQLKERHENHIEKMESIEKLGEARLNLMSPEELIVRIKDRIVQN----RLNYHELYKKYECNDKKGGINKSQLRKMLTQFGYTLTNEQFSKISEILNFPTSSKSRIDFNGFLAIFADNRESSNDLMTDVCFLPKSNHHVNDIRGDDNRNMNCQEVEDLLRQKIVNNFPDLMIAFHKFDRSNKGYLVKSDFQ---NFLLGNLIMIENDQFDLLCEKLGVGEKSKLTYNDFLNRFQIRDDMVD--GHKWLDSKHRFNDPSTNAVPDMSAEDVYMLLPEKIYQYFHEISPTFIALVTSGTGFIRFRNLQHLLSRLQISMSKSEMKKLWKRYDTNNRNKLDYHDFLYNLGLLEKFGCGDLMGPSAAIIQNSHNALVDHLVNQETKHQQSVDNQAVLGTHLHVEHLFEMVKRH-LRVAYSDSALAFKAVANKKTLSGFFTINDLNKLLSQLHMFVTNDKFVQLAARMGFEGPDDSLSYEKFCCIFQDSLNIDGQEKTVGSIRINQDLENPSKIGIEEAGNLNSTQIASIIGKRIVDQYSEIEKTFLVMDPENSGYVSMNEFRNLLVGFCLSLTDKQFNKYRNSLSTYIVKSANTDDKIDYRYFMKGF----NQKLNNLDIQXXXXXXXXXXXXXTYQQIQEHINESIKVRPKLFLNSFKSKDYASLNFISKSDFREIIERFLFTVPDSHFEKFWSNLKKNEFDNVNYGEFV 4064
L +I RN I K F++ D N+ +Q L++V+E FC + + ++K + +++ + V+Y F+K + + D N+ E + G R+ + E+ + + L+ L F ++ + + K DP+ G+++L L +L+ F + + +F Q++ + K + KI W FL + Q ++ T+ +K ++ +++++ L + + Y++ + L I+ T G I E ILN ++ + S + + DP G + + L + + ++ K S + + L ++ ++++ + D+I DL TG I + +K+ + YL ++ + R + + GRI Y + + V P S ++ S+ Q++E + S EK E +N M+ EE+I +K RI Q R + + K+ + IN+ + RK+L + G + + Q++ ++ L F ++ + + F F D +++ + +P + + D++ ++ +E + +K+ +F D+ AF + D G + DF +L N++ +E ++F L LG+ L + +F N + R D + + K + D +++ E + L K + ++S FI G G +R R++++ L I ++ E +KLW+ YDT R + Y +FL+ LG+ + + + + + TK +Q ++ L ++ +R L Y + + AF A+ K ++ + + K+L + + ++ + L + ++ + +F + S + K ++ +P I + ++ + + + + + F +D E++G+V EF ++L C LTD Q++ + L ++ + I ++YF++ F ++ ++ A P + ++ I + +++ + F++ D N +S+ +FR I R + + D F++ WS L N + Y +F+
Sbjct: 191 LTEKIFRNMRSIRKVFQVMDVNNTGLVQPQELRRVLETFCLRMQDGDYEKFLEQYNIDKTTAVDYNAFLKNLSVKNDASFKYLLSNAAELSRETQQGKNGKRLLDTGSSED-VWKNYSLDDLEKTFCQEFSKSYEKIEKALSAGDPSKGGYISLNYLKVVLDTFIYRLPRRIFIQLIRRFGLKTSTKINWKQFLTAIYERQKLEVS-KTLPLKKRSSTE----ARNRSRKENIIKKLFKYSEDRYTALKKTLLIISSTPSGHITWEELRHILNCMVAKLNDSEFSELKQTFDPEGTGAVRVNSLLDVLD--DSTKVRKMSPS----TDTKTPLPVAWDSVEELVLDSITRNLQAFYSMLQSYDLRDTGTIGKNNFRKVMRVFCPYLSNEHLVRFSSKFQ-EAGSGRILYKKFLLSIGVGIPPPTPPLSSPKVQLSDQFQIEEPGQQDERTQPSGEKTSE--INNMTKEEVIDGLKHRIQQKDPVFRKQFLSISKEPDVK-----INQEEFRKVLERSGMPMNDCQYAMLASKLGF--KNEEGMSYQDFTMGFEDC------MLSGLETVPLQSRTASRTNMDEH-FISAEECLRIFPKKLKESFRDVYSAFFRIDLDRDGIISMHDFHRLLQYLQLNMVDLEFERFLSL---LGLRLSVTLNFREFQNLCEKRPWKSDEAPQRLIRCKQKVADS------ELACEQAHQYLIMKAKTRWADLSKNFIETDNEGNGILRRRDIKNSLYGFDIPLTPREFEKLWQNYDTEGRGYITYQEFLHRLGIR-----------YSPKVHRPYKEDYFNFLGHFTKPKQ---------VQEEIQELQQISEREKLMNHYEEISKAFNAMEKSKPVA----LCRVQKVLQECGCPLKEEELISLLKSLDVSVHNNHIDPVEFLRALEISWASKARPK-------EKEESSPPPISF---SKVTPDEVIKTMQEVVESSQPALVEAFSALDKEDTGFVKAMEFGDVLRSVCQKLTDNQYHYFLRRLRLHLTPN------IHWKYFLENFSTFQDETADDWAENMPKAPPPMSPKETAHRDIVARVQKAVASHYHTIVQEFENFDTLKSNTVSRDEFRSICTRHIQILTDEQFDRLWSELPVNAKGRLKYQDFL 1255 HSP 2 Score: 115.161 bits (287), Expect = 5.069e-25 Identity = 166/788 (21.07%), Postives = 302/788 (38.32%), Query Frame = 3
Query: 663 DIIPILQMQISRNPNGIMKAFKLFDFNSDYCIQRHRLKKVMENFCCPLTEQQFDKLVARFSQSQNGNVNYMEFMKGMGIPID-NQGTIQTKNSEETINIDTRNTKGSRIFEAALIENGLLGSNELEQLLVIFRKKIEDQFSVLRKTFLVMDPNAEGFVTLEILYKILNQFAFPMSLNVFNQIMDKCAFKATGKIIWLSFLQKFQ---DPQSKHLNGSTIAIKSDHKCNPIQGVNSKP--------------NIDDLLELLSNHISNNYSSFRDAFLQINQTHKGKIPKSEFLKILNRFQFYISADQLSIILKFLDPHNEGYISYHHFLQLFEQKENLSAHKWLNSNHKVVEPRKDLQLSSETIKSTISDNIGNKWSNVVHEFNLVDLNRTGYISVEDLKKIFKKLFGYLKDDDIDRLVAPLTLKDKKGRINYLELFNLLNVDTSPSDLEGLSTQIAESNDAQLKERHENHIEKME-SIEKLGEARLNLMSPEELIVRIKDRIVQNRLNYHELYKKYECNDKKGGINKSQLRKMLTQFGYTLTNEQFSKISEILNFPTSSKSRIDFNGFLAIFADNRESSNDLMTDVCFLPKSNHHVNDIRGDDNR------------NMNCQEVEDLLRQKIVNNFPDLMIAFHKFDRSNKGYLVKSDFQNFLLGNLIMIENDQFDLLCEKLGVGEKSKLTYNDFLNRFQIRDDMVDGHKWLDSKHRFNDPSTNAVPDMSAEDVYMLLPEKIYQYFHEISPTFIALVTSGTGFIRFRNLQHLLSRLQISMSKSEMKKL 2933
DI IL +IS + I K F++ D N + + + LK+V+ F PLT+ QF L+A+ S GNV Y+EF+ G ID N I+ N E +D R T + E L E IFR +RK F VMD N G V + L ++L F M + + +++ T + + +FL+ D K+L + + + + QG N K ++DDL + S +Y A + + G I + +L+ F + + +++ I++ FL +++ L K L + ++ IK + ++++ + ++ +G+I+ E+L+ I + L D + L + G + L ++L+ T + KM S + + S EEL++ D I +N ++ + + Y+ D G I K+ RK++ F L+NE + S F + RI + FL ++ + + D R NM +EV D L+ +I P F + + + +F+ L + + + + Q+ +L KLG + ++Y DF F+ D M+ G + + + R + +SAE+ + P+K+ + F ++ F + G I + LL LQ++M E ++
Sbjct: 85 DIERILAQKISSRRDDIKKVFQILDRNHNQMVTKGDLKRVITAFLIPLTKDQFQDLLAQIPISSLGNVPYLEFLSRFGGGIDININGIKRVNENE---VDNRRT----VKEVQLTEK-------------IFR-----NMRSIRKVFQVMDVNNTGLVQPQELRRVLETFCLRMQDGDYEKFLEQYNIDKTTAVDYNAFLKNLSVKNDASFKYLLSNAAELSRETQ----QGKNGKRLLDTGSSEDVWKNYSLDDLEKTFCQEFSKSYEKIEKALSAGDPSKGGYISLNYLKVVLDTFIYRLPRRIFIQLIRRFGLKTSTKINWKQFLTAIYERQKLEVSKTLPLKKRSSTEARNRSRKENIIKKLFKYS-EDRYTALKKTLLIISSTPSGHITWEELRHILNCMVAKLNDSEFSELKQTFD-PEGTGAVRVNSLLDVLDDSTK--------------------------VRKMSPSTDTKTPLPVAWDSVEELVL---DSITRNLQAFYSMLQSYDLRD-TGTIGKNNFRKVMRVFCPYLSNEHLVRFSS--KFQEAGSGRILYKKFLLSIGVGIPPPTPPLSSPKVQLSDQFQIEEPGQQDERTQPSGEKTSEINNMTKEEVIDGLKHRIQQKDPVFRKQFLSISKEPDVKINQEEFRKVLERSGMPMNDCQYAMLASKLGFKNEEGMSYQDFTMGFE--DCMLSGLETVPLQSRTASRTNMDEHFISAEECLRIFPKKLKESFRDVYSAFFRIDLDRDGIISMHDFHRLLQYLQLNMVDLEFERF 807 HSP 3 Score: 90.1225 bits (222), Expect = 2.467e-17 Identity = 188/1081 (17.39%), Postives = 410/1081 (37.93%), Query Frame = 3
Query: 690 ISRNPNGIMKAFKLFDFNSDYCIQRHRLKKVMENFCCPLTEQQFDKLVARFSQSQNGNVNYMEFMKGMGIPIDNQGTIQTKNS---EETINIDTRNTKGSRIFEAALIENGLLGSNELEQLLVIFRKKIEDQFSVLRKTFLVMDPNAEGFVTLEILYKILNQFAFPMSLNVFNQIMDKCAFKATGKIIWLSFLQKFQDPQSKHLNGSTIAIKSDHKCNPIQGVNSKPNIDDLLELLSNHISNNYSSFRDAFLQINQTHKGKIPKSEFLKILNRFQFYISADQLSIILKFLDPHNEGYISYHHFLQLFEQ---KENLSAHKWLNSNHKVVEPRKDLQLSSETIKSTISDNIGNKWSNVVHEFNLVDLNRTGYISVEDLKKIFKKLFGYLKDDDIDRLVAPLTLK---------DKKGR--INYLELFNLLNVDTSP-------SDLEGLSTQIAESNDAQLKERHENHIEKMESIEKLGEARLNLMSPEELIVRIKDRIVQNRLNYHELYKKYECNDKKGGINKSQLRKMLTQFGYTLTNEQFSKISEILNFPTSSKSRIDFNGFL-------AIFADNRESSNDLMTDVCFLPKSNHHVNDIRGDDNRNMNCQEVEDLLRQKIVNNFPDLMIAFHKFDRSNKGYLVKSDFQNFLLGNLIMIENDQFDLLCEKLGVGEKSKLTYNDFLNRFQ-IRDDMVDGHKWLDSKHRFNDPSTNAVPDMSAEDVYMLLPEKIYQYFHEISPTFIALVTSGTGFIRFRNLQHLLSRLQISMSKSEMKKLWKRYDTNNRNKLDYHDFLYNLGLLE----KFGCGDLMGPSAAIIQNSHNALVDHLVNQETKHQQSVDNQAVLGTHLH------------VEHLFEMVKRHLRVAYSDSALAFKAVANKKTLSGFFTINDLNKLLSQLHMFVTNDKFVQLAARMGFEGPDDSLSYEKFCCIFQDSLNIDGQEKTVGSIRINQDLENPSKIGIEEAGNLNSTQIASI--IGKRIVDQYSEIEKTFLVMDPENSGYVSMNEFRNLLVGFCLSLTDKQF----NKYRNSLSTYIVKSANTDDKIDYRYFMKGFNQ 3770
I+RN + +D I ++ +KVM FC L+ + + ++F ++ +G + Y +F+ +G+ I + + I+ + R + + + + + E+++ + +I+ + V RK FL + + + E K+L + PM+ + + K FK + + F F+D L + ++ + N + S ++ L + + ++ AF +I+ G I +F ++L Q + + L L +++ F L E+ K + + + + KV D +L+ E + +W+++ F D G + D+K L+G+ DI PLT + D +GR I Y E + L + SP D + Q E+++ ++++ E R LM+ +Y E+ K + +K + +++K+L + G L E+ + + L+ + + ID FL A A +E + F + EV +++ + ++ P L+ AF D+ + G++ +F + L + ++Q+ +L + + + FL F +D+ D W ++ + P + + + D+ + + + ++H I F T + + + + +R ++ + +LW N + +L Y DFL L + GD + + Q +A + + D++ L + H E + +++ ++ + + K K G + + L+ + + ++ ++ QL + + + +Y C F S + + K +R ++N K ++EAG + +++ I +IV + + ++F D +G +S+ +FR +L + ++L++++F Y SLS+ KI Y F++ F Q
Sbjct: 526 ITRNLQAFYSMLQSYDLRDTGTIGKNNFRKVMRVFCPYLSNEHLVRFSSKFQEAGSGRILYKKFLLSIGVGIPPPTPPLSSPKVQLSDQFQIEEPGQQDERT-QPSGEKTSEINNMTKEEVIDGLKHRIQQKDPVFRKQFLSISKEPDVKINQEEFRKVLERSGMPMNDCQYAMLASKLGFKNEEGMSYQDFTMGFEDCMLSGLETVPLQSRTASRTNMDEHFIS---AEECLRIFPKKLKESFRDVYSAFFRIDLDRDGIISMHDFHRLLQYLQLNMVDLEFERFLSLLGLRLSVTLNFREFQNLCEKRPWKSDEAPQRLIRCKQKVA----DSELACEQAHQYLIMKAKTRWADLSKNFIETDNEGNGILRRRDIK---NSLYGF----DI-----PLTPREFEKLWQNYDTEGRGYITYQEFLHRLGIRYSPKVHRPYKEDYFNFLGHFTKPKQVQ---------EEIQELQQISE-REKLMN-----------------HYEEISKAFNAMEKSKPVALCRVQKVLQECGCPLKEEELISLLKSLDVSVHN-NHIDPVEFLRALEISWASKARPKEKEESSPPPISF----------------SKVTPDEVIKTMQEVVESSQPALVEAFSALDKEDTGFVKAMEFGDVLRSVCQKLTDNQYHYFLRRLRLHLTPNIHWKYFLENFSTFQDETADD--WAENMPK--APPPMSPKETAHRDIVARVQKAVASHYHTIVQEFENFDTLKSNTVSRDEFRSICTRHIQILTDEQFDRLWSELPVNAKGRLKYQDFLSKLSIERVPSPPMAAGD--SGESTMAQRGSSAPEFSQGTRSNLYSPPRDSRVGLKSRSHPCTPVGTPPLQNCEPIESRLRKQIQGCWRELLRECKEKDTDK--QGTISAAEFLALVEKFKLDISREESQQLIVKYDLKN-NGKFAY----CDFIQSCVLLLKAKETSLMR-RMRIQNADK--MKEAGMETPSFYSALLRIQPKIVHCWRPMRRSFKTYDKNGTGLLSVADFRKVLRQYSINLSEEEFFHVLEYYDKSLSS----------KISYNDFLRAFLQ 1516 HSP 4 Score: 85.8853 bits (211), Expect = 4.035e-16 Identity = 106/554 (19.13%), Postives = 231/554 (41.70%), Query Frame = 3
Query: 2436 EVEDLLRQKIVNNFPDLMIAFHKFDRSNKGYLVKSDFQNFLLGNLIMIENDQFDLLCEKLGVGEKSKLTYNDFLNRFQIRDDM-VDGHKWLDSKHRFNDPSTNAVPDMSAEDVYMLLPEKIYQYFHEISPTFIALVTSGTGFIRFRNLQHLLSRLQISMSKSEMKKLWKRYDTNNRNKLDYHDFLYNLGLLEKFGCGDLMGPSAAIIQNSHNA-----LVDHLVNQETKHQQSVDNQAVLGTHLHVEHLF--EMVKRHLRVAYSDSALAFKAVANKKTLSGFFTINDLNKLLSQLHMFVTNDKFVQLAARMGFEGPDDSLSYEKFCCIFQDSLNIDGQEKTVGSIRINQDLENPSKIGIEEAGNLNSTQIASIIGKRIVDQYSEIEKTFLVMDPENSGYVSMNEFRNLLVGFCLSLTDKQFNKYRNSL---STYIVKSANTDDKIDYRYFMKGFNQKLNNLDIQXXXXXXXXXXXXXTYQQIQEHINESIKVRPKLFLNSFKSKDYASLNFISKSDFREIIERFLFTVPDSHFEKFWSNLKKNEFDNVNYGEFV 4064
++E +L QKI + D+ F DR++ + K D + + LI + DQF L ++ + + Y +FL+RF D+ ++G K ++ N + V L EKI++ I F + + TG ++ + L+ +L + M + +K ++Y+ + +DY+ FL NL + L+ +A + + + L+D +++ S+D+ +E F E K + ++ + AL+ A + G+ ++N L +L + F+QL R G + +++++F + ++ +++ L + E I + K D+Y+ ++KT L++ SG+++ E R++L L D +F++ + + T V+ + D +D ++ + + + + ++E + +SI + F + +S D I K++FR+++ F + + H +F S ++ + Y +F+
Sbjct: 85 DIERILAQKISSRRDDIKKVFQILDRNHNQMVTKGDLKRVITAFLIPLTKDQFQDLLAQIPISSLGNVPYLEFLSRFGGGIDININGIKRVNENEVDNRRTVKEVQ----------LTEKIFRNMRSIRKVFQVMDVNNTGLVQPQELRRVLETFCLRMQDGDYEKFLEQYNIDKTTAVDYNAFLKNLSVKNDASFKYLLSNAAELSRETQQGKNGKRLLDTGSSEDVWKNYSLDD---------LEKTFCQEFSKSYEKI---EKALS----AGDPSKGGYISLNYLKVVLDTFIYRLPRRIFIQLIRRFGLKT-STKINWKQFLTAIYERQKLE----------VSKTLPLKKRSSTEARNRSRKENIIKKLFKYSEDRYTALKKTLLIISSTPSGHITWEELRHILNCMVAKLNDSEFSELKQTFDPEGTGAVRVNSLLDVLDDSTKVRKMSPSTDT-----------KTPLPVAWDSVEELVLDSITRNLQAFYSMLQSYDLRDTGTIGKNNFRKVMRVFCPYLSNEHLVRFSSKFQEAGSGRILYKKFL 590 HSP 5 Score: 83.5741 bits (205), Expect = 1.956e-15 Identity = 110/460 (23.91%), Postives = 192/460 (41.74%), Query Frame = 3
Query: 225 QLLKNHKEILKALQTFDIECSGLVTKGELRRVLDSYCLPMTTDQYEQFLSHNSINYSKGSINYKELIDKIYDKTSAVGH--FKKNIKLPQLQRSATTICLQNKHFTLPTTRRSSGKISRSQLFXXXXXXXXXXXXXXXXWNYNAYTIDDIIPILQMQISRNPNGIMKAFKLFDFNSDYCIQRHRLKKVMENFCCPLTEQQFDKLVARFSQSQNGNVNYMEFMKGMGIPIDNQGTIQTKNSEETINIDTRNTKGSRIFEAALIENGLLGSNELEQLLVIFRKKIEDQFSVLRKTFLVMDPNAEGFVTLEILYKILNQFAFPMSLNVFNQIMDKCAFKATGKIIWLSFLQKFQDP-QSKHLNGSTIAIKSDHKCN-PIQGVNSKPNIDDLLELLSNHISNNYSSFRDAFLQINQTHKGKIPKSEFLKILNRFQFYISADQLSIILKFLDPHNEGYISYHHFL 1592
++ +N + I K Q D+ +GLV ELRRVL+++CL M YE+FL +I DKT+AV + F KN+ + ++ L N + ++SR + R L++ S W Y++DD+ + S++ I KA D + I + LK V++ F L + F +L+ RF + +N+ +F+ + Q +T+ + R++ +R N N +++L K ED+++ L+KT L++ G +T E L ILN ++ + F+++ + TG + S L D + + ++ ST D K P+ D + EL+ + I+ N +F + G I K+ F K++ F Y+S + L G I Y FL
Sbjct: 194 KIFRNMRSIRKVFQVMDVNNTGLVQPQELRRVLETFCLRMQDGDYEKFLEQYNI-----------------DKTTAVDYNAFLKNLSVK--NDASFKYLLSN-----------AAELSRETQQGKNGKRLLDTGSSEDVW--KNYSLDDLEKTFCQEFSKSYEKIEKALSAGDPSKGGYISLNYLKVVLDTFIYRLPRRIFIQLIRRFGLKTSTKINWKQFLTAI-------YERQKLEVSKTLPLKKRSSTEAR--------NRSRKENIIKKLF----KYSEDRYTALKKTLLIISSTPSGHITWEELRHILNCMVAKLNDSEFSELKQTFDPEGTGAVRVNSLLDVLDDSTKVRKMSPST-----DTKTPLPVAW-------DSVEELVLDSITRNLQAFYSMLQSYDLRDTGTIGKNNFRKVMRVFCPYLSNEHLVRFSSKFQEAGSGRILYKKFL 590 HSP 6 Score: 75.485 bits (184), Expect = 5.976e-13 Identity = 180/1085 (16.59%), Postives = 412/1085 (37.97%), Query Frame = 3
Query: 204 INPIVKSQLLKNHKEILKALQTFDIECSGLVTKGELRRVLDSYCLPMTTDQYEQFLSHNSINYSKGSINYKELIDKIYDKTSAVGHFKKNIKLPQLQRSATTICLQNKHFTLPTTRRSSGKISRSQLFXXXXXXXXXXXXXXXXWNYNAYTIDDIIPILQMQISRNPNGIMKAFKLFDFNSDYCIQRHRLKKVMENFCCPLTEQQFDKLVARFSQSQNGNVNYMEFMKGMGIPIDNQGTIQTKNSEETINIDTRNTKGSRIFEAALIENGLLGSNELEQLLVIFRKKIEDQFSVLRKTFLVMDPNAEGFVTLEILYKILNQFAFPMSLNVFNQIMDKCAFKATGKIIWLSFLQKFQDPQSKHLNGSTIAIKSDHKCNPIQGVNSKPNIDDLLELLSNHISNNYSSFRDAFLQINQTHKGKIPKSEFLKILNRFQFYISADQLSIILKFLDPHNEGYISYHHFLQLFEQKENLSAHK-----WLNSNHKVVEPRKDLQLSSETIKSTISDNIGNKWSNVVHEFNLVDLNRTGYISVEDLKKIFKKLFGYLKDDDIDRLVAPLTLKDKKGRINYLELFNLLNVDTSPSDLEGLSTQIAESNDAQLKERHENHIEKMESIEKLGEARLNLMSPEELIVRIKDRIVQNRLNYHELYKKYECNDKKGGINKSQLRKMLTQFGYTLTNEQFSKISEILNFPTSSKSRIDFNGFLAIFAD-NRESSNDLMTDVCFLPKSNHHVNDIRGDDNRNMNCQEVEDLLRQKIVNNFPDLMIAFHKFDRSNKGYLVKSDFQNFLLGNLIMIENDQFDLLCEKLGVGEKSKLTYNDFLNRFQI--------------------------------RDDMV----DGHKWLDSKHRFNDPSTNAVPDMSAEDVYMLLPEKIYQYFHEISPTFIALVTSGTGFIRFRNLQHLLSRLQISMSKSEMKKLWKRYDTNNRNKLDYHDFLYNLGLLEKFGCGDLMGPSAAIIQNSHNALVDHLVNQETKHQQSVDNQAVLGTHLHVEHLFEMVKRHLRVAYSDSALAFKAVANKKTLSGFFTINDLNKLLSQLHMFVTNDKFVQL 3332
+ +V + +N + LQ++D+ +G + K R+V+ +C P ++++ S G I YK+ + I + K+ + Q T P+ ++S N T +++I L+ +I + K F D I + +KV+E P+ + Q+ L ++ ++Y +F G + + ET+ + +R + + E + E+ L IF KK+++ F + F +D + +G +++ +++L M F + + + + + L+F ++FQ+ K S A + +C + +S+ + + L ++ F++ + G + + + L F ++ + + + D GYI+Y FL + + H+ + N +P++ + E + + + + N + + FN ++ ++ +++ ++K+ ++ LK++++ L+ L + I+ +E L +I+ ++ A+ KE+ E+ + + ++P+E+I +++ + ++ E + + D G + + +L LT+ Q+ L + I + FL F+ E+++D + +PK+ ++ + +++ +++ + +++ ++ F FD + + +F++ ++ ++ ++QFD L +L V K +L Y DFL++ I R ++ D L S+ P P + E + L ++I + E+ T G I L+ + ++ +S+ E ++L +YD N K Y DF+ + LL K LM IQN+ +E + A+L + H + ++R +FK K +G ++ D K+L Q + ++ ++F +
Sbjct: 518 VEELVLDSITRNLQAFYSMLQSYDLRDTGTIGKNNFRKVMRVFC-PYLSNEHLVRFSSKFQEAGSGRILYKKFLLSIGVGIPPPTPPLSSPKVQLSDQFQIEEPGQQDERTQPSGEKTS--------------------------EINNMTKEEVIDGLKHRIQQKDPVFRKQFLSISKEPDVKINQEEFRKVLERSGMPMNDCQYAMLASKLGFKNEEGMSYQDFTMGFEDCM--------LSGLETVPLQSRTASRTNMDEHFI---------SAEECLRIFPKKLKESFRDVYSAFFRIDLDRDGIISMHDFHRLLQYLQLNMVDLEFERFLSLLGLRLS---VTLNF-REFQNLCEKRPWKSDEAPQRLIRCKQ-KVADSELACEQAHQYLIMKAKTRWADLSKNFIETDNEGNGILRRRDIKNSLYGFDIPLTPREFEKLWQNYDTEGRGYITYQEFLHRLGIRYSPKVHRPYKEDYFNFLGHFTKPKQVQEEIQELQQISEREKLMNHYEEISKAFNAME--KSKPVALCRVQKVLQECGCPLKEEELISLLKSLDVSVHNNHIDPVEFLRAL--------------EISWASKARPKEKEESSPPPIS---------FSKVTPDEVIKTMQEVVESSQPALVEAFSALDKEDT-GFVKAMEFGDVLRSVCQKLTDNQYHYFLRRLRLHLTPN--IHWKYFLENFSTFQDETADDWAEN---MPKAPPPMSP------KETAHRDIVARVQKAVASHYHTIVQEFENFDTLKSNTVSRDEFRSICTRHIQILTDEQFDRLWSELPVNAKGRLKYQDFLSKLSIERVPSPPMAAGDSGESTMAQRGSSAPEFSQGTRSNLYSPPRDSRVGLKSRSHPCTP-VGTPPLQNCEPIESRLRKQIQGCWRELLRECKEKDTDKQGTISAAEFLALVEKFKLDISREESQQLIVKYDLKNNGKFAYCDFIQSCVLLLKAKETSLM--RRMRIQNADKM-------KEAGMETPSFYSALLRIQPKIVHCWRPMRR-----------SFKTY--DKNGTGLLSVADFRKVLRQYSINLSEEEFFHV 1493 HSP 7 Score: 65.855 bits (159), Expect = 6.087e-10 Identity = 49/213 (23.00%), Postives = 97/213 (45.54%), Query Frame = 3
Query: 4155 IETDEMDTDESIRKNLERLLNRNWQSIFNKCKAIDQAINECQTAEGSIPIDDFVYILSEYDDSLS-AENLAAINLYKTCKNGRFIKYGDLIRSIVLSSRGSLISNENVTEVQK--KFLNVGIKTTNSDNSKDYFPQLFKLRRQILENWKDLRKNFLQSDKKQNGAVSGNIFMTIMKTVCGQLSADLFYHLMKIYDQDGTGNFHYKRFIEDFLK 4784
+ T + E I L + + W+ + +CK D +G+I +F+ ++ ++ +S E+ I Y NG+F Y D I+S VL + S +Q K G++T + ++ L +++ +I+ W+ +R++F DK G +S F +++ LS + F+H+++ YD+ + Y F+ FL+
Sbjct: 1317 VGTPPLQNCEPIESRLRKQIQGCWRELLRECKEKDT------DKQGTISAAEFLALVEKFKLDISREESQQLIVKYDLKNNGKFA-YCDFIQSCVLLLKAKETSLMRRMRIQNADKMKEAGMETPS------FYSALLRIQPKIVHCWRPMRRSFKTYDKNGTGLLSVADFRKVLRQYSINLSEEEFFHVLEYYDKSLSSKISYNDFLRAFLQ 1516
BLAST of EF-hand calcium-binding domain-containing protein 6 vs. UniProt/SwissProt
Match: sp|Q5THR3|EFCB6_HUMAN (EF-hand calcium-binding domain-containing protein 6 OS=Homo sapiens OX=9606 GN=EFCAB6 PE=1 SV=1) HSP 1 Score: 219.164 bits (557), Expect = 4.324e-56 Identity = 255/1152 (22.14%), Postives = 481/1152 (41.75%), Query Frame = 3
Query: 687 QISRNPNGIMKAFKLFDFNSDYCIQRHRLKKVMENFCCPLTEQQFDKLVARFSQSQNGNVNYMEFMKGMGIPIDNQGTIQTKNSEETI-NIDTRNTKGSRIFEAALIENGLLGSNELEQLLVIFRKKIEDQFSVLRKTFLVMDPNAEGFVTLEILYKILNQFAFPMSLNVFNQIMDKCAFKATGKIIWLSFLQKFQDPQSKHLNGSTIAIKSDHKCNPIQGVNSK--PNIDDLLELLSNHISNNYSSFRDAFLQINQTHKGKIPKSEFLKILNRFQFYISADQLSIILKFLDPHNEGYISYHHFLQLFEQKENLSAHKWLNSNHKVVEPRKDLQLSSETIKSTISDNIGNKWSNVVHEFNLVDLNRTGYISVEDLKKIFKKLFGYLKDDDIDRLVAPLTLKD-KKGRINYLELFNLLNVDTSPSDLEGLSTQIAESNDAQLKERHENHIEKMESIEKLGEARLNLMS----------PEELIVRIKDRIVQNRLNYHELYKKYECNDKKGGINKSQLRKMLTQFGYTLTNEQFSKISEILNFPTSSKSRIDFN-GFLAIFADNRESSNDLMTDVCFLPKSNHHVNDIRGDDNRNMNCQEVEDLLRQKIVNNFPDLMIAFHKFDRSNKGYLVKSDFQNFLLGNLIMIENDQFDLLCEKLGVGEKSKLTYNDFLNRFQIRDDMVDGHKWLDSKHRFNDPSTN-AVPDMSAEDVYMLLPEKIYQYFHEISPTFIALVTSGTGFIRFRNLQHLLSRLQISMSKSEMKKLWKRYDTNNRNKLDYHDFLYNLGLLEKFGCGDLMGPSAAIIQNSHNALVDHLVN---QETKHQQSVDNQAVL--GTHLHVEHLFEMVKRHLRVAYSDSALAFKAVANKKTLSGFFTINDLNKLLSQLHMFVTNDKFVQLAARMGFEGPDDSLSYEKFCCIFQDSLNIDGQEK-TVGSIRINQDLENPSKIGIEEAGNLNSTQIASIIGKRIVDQYSEIEKTFLVMDPENSGYVSMNEFRNLLVGFCLSLTDKQFNKYRNSLSTYIVKSANTDDKIDYRYFMKGFN----QKLNNLDIQXXXXXXXXXXXXXTYQQIQEHINESIKVRPKLFLNSFKSKDYASLNFISKSDFREIIERFLFTVPDSHFEKFWSNLKKNEFDNVNYGEFV 4064
++ +N +MKAF+L D N ++ L++V+E FC L +++++K ++ ++ V+Y F+K + I D N E ++ N +N+K R+ +A E+ + + L+++ F ++ + + K DP G+V+ L +L+ F + + +F Q+M + KAT KI W FL F +PQ ++ K + +NS+ + ++++ L H ++ +S + A L IN G I + EF ILN +S + +++ LDP + G ++ F+ L E EN K + + L+ ++++ + D I + DL TG I + KKI +L + +L + ++D GRI Y +L + +D P+ + + D L E H++K E + R L EE+I + K I Q + + + + + G IN +K+L G + ++Q++ ++ + F S +DF GF ++ T S +VN + + +E L +++ +F D AF K D G + D LL L+ +++D+F+ LG+ L + +F N + R D ++ R P A +++ E + L K + ++S F+ G G +R R++++ L I ++ E +KLW RYDT + + Y +FL LG+ S A+ H + N TK QQ + L T V +++ RH D + AF KT + +I + ++L + +T + L G D++++Y F ++S + Q K S+ IN NP + + + S+Q+A + F +D E++G+V EF +L FC LTD Q++ + L ++ I+++YF++ F+ + + + P + + I +++++ F++ D N IS+ +FR I R + + D F++ W+ + N + Y +F+
Sbjct: 169 KVFKNIKTVMKAFELIDVNKTGLVRPQELRRVLETFCMKLRDEEYEKFSKHYNIHKDTAVDYNVFLKNLSINNDLNLRYCMGNQEVSLENQQAKNSKKERLLGSASSED-IWRNYSLDEIERNFCLQLSKSYEKVEKALSAGDPCKGGYVSFNYLKIVLDTFVYQIPRRIFIQLMKRFGLKATTKINWKQFLTSFHEPQGLQVSSKGPLTKRN-------SINSRNESHKENIITKLFRHTEDHSASLKKALLIINTKPDGPITREEFRYILNCMAVKLSDSEFKELMQMLDPGDTGVVNTSMFIDLIE--ENCRMRK----TSPCTDAKTPFLLAWDSVEEIVHDTITRNLQAFYNMLRSYDLGDTGRIGRNNFKKIMHVFCPFLTNAHFIKLCS--KIQDIGSGRILYKKLLACIGIDGPPT-----VSPVLVPKDQLLSE----HLQKDEQQQPDLSERTKLTEDKTTLTKKMTTEEVIEKFKKCIQQQDPAFKKRFLDFS-KEPNGKINVHDFKKVLEDTGMPMDDDQYALLTTKIGFEKEGMSYLDFAAGF-----EDPPMRGPETTPPQPPTPSKSYVN------SHFITAEECLKLFPRRLKESFRDPYSAFFKTDADRDGIINMHDLHRLLLHLLLNLKDDEFERFLGLLGLRLSVTLNFREFQNLCEKRPWRTD-----EAPQRLIRPKQKVADSELACEQAHQYLVTKAKNRWSDLSKNFLETDNEGNGILRRRDIKNALYGFDIPLTPREFEKLWARYDTEGKGHITYQEFLQKLGI----------NYSPAV----HRPCAEDYFNFMGHFTKPQQLQEEMKELQQSTEKAVAARDKLMDRH-----QDISKAFTKTDQSKT--NYISICKMQEVLEECGCSLTEGELTHLLNSWGVSRHDNAINYLDFLRAVENSKSTGAQPKEKEESMPINFATLNPQEAVRKIQEVVESSQLA-------------LSTAFSALDKEDTGFVKATEFGQVLKDFCYKLTDNQYHYFLRKLRIHLTPY------INWKYFLQNFSCFLEETADEWAEKMPKGPPPTSPKATADRDILARLHKAVTSHYHAITQEFENFDTMKTNTISREEFRAICNRRVQILTDEQFDRLWNEMPVNAKGRLKYPDFL 1238 HSP 2 Score: 83.9593 bits (206), Expect = 1.233e-14 Identity = 126/678 (18.58%), Postives = 265/678 (39.09%), Query Frame = 3
Query: 1380 ELLSNHISNNYSSFRDAFLQINQTHKGKIPKSEFLKILNRFQFYISADQLSIILKFLDPHNEGYISYHHFLQLFEQKENLSAHKWLNSNH-----------KVVEPRKDLQLSSETIKSTISDNIGNKWSNVVHEFNLVDLNRTGYISVEDLKKIFKKLFGYLKDDDIDRLVAPLTLKDKKGRINYLELFNLLNVDTSPSDLEGLSTQIAESNDAQLKERHENHIEKMESIEKLGEARLNLMSPEELIVRIKDRIVQNRLNYHELYKKYECNDKKGGINKSQLRKMLTQFGYTLTNEQFSKISEILNFPTSSKSRIDFNGFLAIFADNRESSNDLMTDVCFLPKSNHHVNDIRGDDNRNMNCQEVEDLLRQKIVNNFPDLMIAFHKFDRSNKGYLVKSDFQNFLLGNLIMIENDQFDLLCEKLGVGEKSKLTYNDFLNRFQIR--------------------DDMVDGHKWLDS-------------KHRFNDPSTNAVPDMS----AEDVYMLLPEKIYQYFHEISPTFIALVTSGTGFIRFRNLQHLLSRLQISMSKSEMKKLWKRYDTNNRNKLDYHDFLYNLGLLEKFGCGDLMGPSAAIIQNSHNALVDHLVNQETKHQQSVDNQAVLGTHLHVEHLFEMVKRHLRVAYSDSALAFKAVANKKTLSGFFTIN 3269
+ L N +S FL+ + G + + + L F ++ + + D +G+I+Y FLQ + + H+ ++ ++ E K+LQ S+E + D + ++ ++ F D ++T YIS+ ++++ ++ L + ++ L+ + INYL+ + + ++S AQ KE+ E+ + A LN P+E + +I++ + ++L + + D G + ++ ++L F Y LT+ Q+ L + I++ FL F+ E + D + +PK + D +++ L + + +++ + F FD + + +F+ + ++ ++QFD L ++ V K +L Y DFL+RF D+ +G + S H ST +P + + L ++I + ++ + G I + L+ + + +SK E ++L +YD + K Y DF+ + LL K LM IQN+H +E + A+L + H + ++R + +Y ++ +VA+ +T+ ++IN
Sbjct: 839 QYLVTKAKNRWSDLSKNFLETDNEGNGILRRRDIKNALYGFDIPLTPREFEKLWARYDTEGKGHITYQEFLQKLGINYSPAVHRPCAEDYFNFMGHFTKPQQLQEEMKELQQSTEKAVAA-RDKLMDRHQDISKAFTKTDQSKTNYISICKMQEVLEECGCSLTEGELTHLLNSWGVSRHDNAINYLDFLR--------------AVENSKSTGAQPKEKEESM--------PINFATLN---PQEAVRKIQEVVESSQLALSTAFSALDKEDT-GFVKATEFGQVLKDFCYKLTDNQYHYFLRKLRIHLTP--YINWKYFLQNFSCFLEETADEWAEK--MPKGPPPTSPKATAD------RDILARLHKAVTSHYHAITQEFENFDTMKTNTISREEFRAICNRRVQILTDEQFDRLWNEMPVNAKGRLKYPDFLSRFSSETAATPMATGDSAVAQRGSSVPDVSEGTRSALSLPTQELRPGSKSQSHPCTPASTTVIPGTPPLQNCDPIESRLRKRIQGCWRQLLKECKEKDVARQGDINASDFLALVEKFNLDISKEECQQLIIKYDLKSNGKFAYCDFIQSCVLLLKAKESSLM--HRMKIQNAHKM-------KEAGAETPSFYSALLRIQPKIVHCWRPMRRTFK-SYDEAGTGLLSVADFRTVLRQYSIN 1469 HSP 3 Score: 76.2554 bits (186), Expect = 2.599e-12 Identity = 99/475 (20.84%), Postives = 196/475 (41.26%), Query Frame = 3
Query: 195 ISEINPIVKSQLLKNHKEILKALQTFDIECSGLVTKGELRRVLDSYCLPMTTDQYEQFLSHNSINYSKGSINYKELIDKIYDKTSAVGH--FKKNIKLPQLQRSATTICLQNKHFTLPTTRRSSGKISRSQLFXXXXXXXXXXXXXXXXWNYNAYTIDDIIPILQMQISRNPNGIMKAFKLFDFNSDYCIQRHRLKKVMENFCCPLTEQQFDKLVARFSQSQNGNVNYMEFMKGM----GIPIDNQGTIQTKNSEETINIDTRNTKGSRIFEAALIENGLLGSNELEQLLVIFRKKIEDQFSVLRKTFLVMDPNAEGFVTLEILYKILNQFAFPMSLNVFNQIMDKCAFKATGKIIWLSFLQKFQDPQSKHLNGSTIAIKSDHKCNPIQGVNSKPNI---DDLLELLSNHISNNYSSFRDAFLQINQTHKGKIPKSEFLKILNRFQFYISADQLSIILKFLDPHNEGYISYHHFL 1592
+ E+ V ++ KN K ++KA + D+ +GLV ELRRVL+++C+ + ++YE+F H +I+ K +AV + F KN+ + C+ N+ +L + + K R L S S W Y++D+I +Q+S++ + KA D + + LK V++ F + + F +L+ RF +N+ +F+ G+ + ++G + +NS I++RN + E ++ + ED + L+K L+++ +G +T E ILN A +S + F ++M TG + F+ ++ ++ C + P + D + E++ + I+ N +F + + G+I ++ F KI++ F +++ + + G I Y L
Sbjct: 159 LRELEIQVGEKVFKNIKTVMKAFELIDVNKTGLVRPQELRRVLETFCMKLRDEEYEKFSKHYNIH-----------------KDTAVDYNVFLKNLSIN--NDLNLRYCMGNQEVSLENQQAKNSKKER----------LLGSASSEDIW--RNYSLDEIERNFCLQLSKSYEKVEKALSAGDPCKGGYVSFNYLKIVLDTFVYQIPRRIFIQLMKRFGLKATTKINWKQFLTSFHEPQGLQVSSKGPLTKRNS-----INSRN------------------ESHKENIITKLFRHTEDHSASLKKALLIINTKPDGPITREEFRYILNCMAVKLSDSEFKELMQMLDPGDTGVVNTSMFIDLIEE---------NCRMRKTSPCTDAK----TPFLLAWDSVEEIVHDTITRNLQAFYNMLRSYDLGDTGRIGRNNFKKIMHVFCPFLTNAHFIKLCSKIQDIGSGRILYKKLL 566 HSP 4 Score: 73.9442 bits (180), Expect = 1.313e-11 Identity = 103/552 (18.66%), Postives = 228/552 (41.30%), Query Frame = 3
Query: 2415 NRNMNCQEVEDLLRQKIVNNFPDLMIAFHKFDRSNKGYLVKSDFQNFLLGNLIMIENDQFDLLCEKLGVGEKSKLTYNDFLNRFQIRDDMVDGHKWLDSKHRFNDPSTNAVPDMSAEDVYMLLPEKIYQYFHEISPTFIALVTSGTGFIRFRNLQHLLSRLQISMSKSEMKKLWKRYDTNNRNKLDYHDFLYNLGLLEKFGCGDLMGPSAAIIQNSHNALVDHLVNQETKHQQSVDNQAVLGTH----LHVEHLFEMVKRH--LRVAYSDSALAFKAVANKKTLSGFFTINDLNKLLSQLHMFVTNDKFVQLAARMGFEGPDDSLSYEKFCCIFQDSLNIDGQEKTVGSIRINQDLENPSKIGIEEAGNLNS------TQIASIIGKRIVDQYSEIEKTFLVMDPENSGYVSMNEFRNLLVGFCLSLTDKQFNKYRNSL---STYIVKSANTDDKIDYRYFMKGFNQKLNNLDIQXXXXXXXXXXXXXTYQQIQEHINESIKVRPKLFLNSFKSKDYASLNFISKSDFREIIERFLFTVPDSHFEKFWSNLK 4025
N ++ +V+ +L QKI + +L AF D + KS+ + + L+ + +QF + ++ + + Y FL+RF D ++G K R N + ++ + + EK+++ + F + + TG +R + L+ +L + + E +K K Y+ + +DY+ FL NL + MG + V+ E + ++ + +LG+ + + + ++R+ L+++ S + A G+ + N L +L + F+QL R G + +++++F F + Q L+ SK + + ++NS I + + + D + ++K L+++ + G ++ EFR +L + L+D +F + L T +V ++ D I+ M+ ++ A + + ++E ++++I + F N +S D I +++F++I+ F + ++HF K S ++
Sbjct: 52 NPTLSSLDVKRILFQKITDRGDELQKAFQLLDTGQNLTVSKSELRRIITDFLMPLTREQFQDVLAQIPLSTSGTVPYLAFLSRFGGIDLYINGIK------RGGGNEMNCC--RTLRELEIQVGEKVFKNIKTVMKAFELIDVNKTGLVRPQELRRVLETFCMKLRDEEYEKFSKHYNIHKDTAVDYNVFLKNLSINNDLNLRYCMG--------------NQEVSLENQQAKNSKKERLLGSASSEDIWRNYSLDEIERNFCLQLSKSYEKVEKALSAGDPCKGGYVSFNYLKIVLDTFVYQIPRRIFIQLMKRFGLKA-TTKINWKQFLTSFHEP----------------QGLQVSSKGPLTKRNSINSRNESHKENIITKLFRHTEDHSASLKKALLIINTKPDGPITREEFRYILNCMAVKLSDSEFKELMQMLDPGDTGVVNTSMFIDLIEENCRMR-----------KTSPCTDAKTPFLLAWDSVEEIVHDTITRNLQAFYNMLRSYDLGDTGRIGRNNFKKIMHVFCPFLTNAHFIKLCSKIQ 553 HSP 5 Score: 68.1662 bits (165), Expect = 8.201e-10 Identity = 49/212 (23.11%), Postives = 97/212 (45.75%), Query Frame = 3
Query: 4161 TDEMDTDESIRKNLERLLNRNWQSIFNKCKAIDQAINECQTAEGSIPIDDFVYILSEYDDSLSAENLAAINL-YKTCKNGRFIKYGDLIRSIVL---SSRGSLISNENVTEVQKKFLNVGIKTTNSDNSKDYFPQLFKLRRQILENWKDLRKNFLQSDKKQNGAVSGNIFMTIMKTVCGQLSADLFYHLMKIYDQDGTGNFHYKRFIEDFLK 4784
T + + I L + + W+ + +CK D A +G I DF+ ++ +++ +S E + + Y NG+F Y D I+S VL + SL+ + K G +T + ++ L +++ +I+ W+ +R+ F D+ G +S F T+++ LS + F+H+++ YD+ + Y F+ FL+
Sbjct: 1304 TPPLQNCDPIESRLRKRIQGCWRQLLKECKEKDVA------RQGDINASDFLALVEKFNLDISKEECQQLIIKYDLKSNGKFA-YCDFIQSCVLLLKAKESSLMHRMKIQNAHK-MKEAGAETPS------FYSALLRIQPKIVHCWRPMRRTFKSYDEAGTGLLSVADFRTVLRQYSINLSEEEFFHILEYYDKTLSSKISYNDFLRAFLQ 1501
BLAST of EF-hand calcium-binding domain-containing protein 6 vs. UniProt/SwissProt
Match: sp|Q5RE62|EFCB6_PONAB (EF-hand calcium-binding domain-containing protein 6 OS=Pongo abelii OX=9601 GN=EFCAB6 PE=3 SV=1) HSP 1 Score: 195.667 bits (496), Expect = 2.872e-49 Identity = 230/1042 (22.07%), Postives = 432/1042 (41.46%), Query Frame = 3
Query: 687 QISRNPNGIMKAFKLFDFNSDYCIQRHRLKKVMENFCCPLTEQQFDKLVARFSQSQNGNVNYMEFMKGMGIPIDNQGTIQTKNSEETI-NIDTRNTKGSRIFEAALIENGLLGSNELEQLLVIFRKKIEDQFSVLRKTFLVMDPNAEGFVTLEILYKILNQFAFPMSLNVFNQIMDKCAFKATGKIIWLSFLQKFQDPQSKHLNGSTIAIKSDHKCNPIQGVNSKPNIDDLLELLSNHISNNYSSFRDAFLQINQTHKGKIPKSEFLKILNRFQFYISADQLSIILKFLDPHNEGYISYHHFLQLFEQKENLSAHKWLNSNHKVVEPRKDLQLSSETIKSTISDNIGNKWSNVVHEFNLVDLNRTGYISVEDLKKIFKKLFGYLKDDDIDRLVAPLTLKD-KKGRINYLELFNLLNVDTSPSDLEGL--STQIAESNDAQLKERHENHIEKMESIEKLGEARLNLMSPEELIVRIKDRIVQNRLNYHELYKKYECNDKKGGINKSQLRKMLTQFGYTLTNEQFSKISEILNFPTSSKSRIDFNGFLAIFADNRESSNDLMTDVCFLPKSNHHVNDIRGDDNRNMNCQEVEDLLRQKIVNNFPDLMIAFHKFDRSNKGYLVKSDFQNFLLGNLIMIENDQFDLLCEKLGVGEKSKLTYNDFLNRFQIRDDMVDGHKWLDSKHRFNDPSTN-AVPDMSAEDVYMLLPEKIYQYFHEISPTFIALVTSGTGFIRFRNLQHLLSRLQISMSKSEMKKLWKRYDTNNRNKLDYHDFLYNLGLLEKFGCGDLMGPSAAIIQN--SHNALVDHLVNQETKHQQSVDNQAVLGTHLHVEHLFEMVKRHLRVAYSDSALAFKAVANKKTLSGFFTINDLNKLLSQLHMFVTNDKFVQLAARMGFEGPDDSLSYEKFCCIFQDSLNIDGQEKTVGSIRINQDLENPSKIGIEEAGNLNSTQIASIIGKRIVDQYSEIEKTFLVMDPENSGYVSMNEFRNLLVGFCLSLTDKQFNKYRNSLSTYIVKSANTDDKIDYRYFMKGFNQKLNNLDI 3791
++ +N +MKAFKL D N ++ L++V+E FC L +++++K ++ + V+Y F+K + I D N E ++ N RN+K R+ +A E+ + + L+ + F ++ + + K DP G+V+ L +L+ F + + +F Q+M + KAT KI W FL F +PQ ++ K N I S+ ++++ L + +S + A L IN G I + EF ILN +S + +++ LDP + G ++ F+ L E EN K + + L+ ++++ + D I + DL TG I + KKI +L + +L + ++D GRI Y +L + +D P+ L Q+ + + +++ + E+ + E + + EE+I + + I Q + + + + + G IN RK+L G + ++Q++ ++ + F S +DF A F D + S +VN + + +E L +++ +F D AF K D G + D LL L+ +++D+F+ LG+ L + +F N + R D ++ R P A +++ E + L K + ++S F+ G G +R R++++ L I ++ E +KLW RY+T + + Y +FL LG+ + P A N H L + + QQS + + L + D + AF + KT + +I + K+L + +T + L G +++++Y F ++S + Q K E + I A LN ++ I + + + F +D E++G+V EF +L FC L D Q++ + L ++ I+++YF++ F+ L + I
Sbjct: 17 KVFKNIKTVMKAFKLIDVNKTGLVRPQELRRVLETFCLKLRDEEYEKFSKHYNIHNDTAVDYNVFLKNLSINNDLNLRYCMGNQEVSLENQQARNSKKERLLGSASSED-IWRNYSLDDIERNFCLELSKSYEKIEKALSAGDPCKGGYVSFNYLKIVLDTFIYQIPRRIFIQLMKRFGLKATTKINWKQFLTSFHEPQGLQVSNKGPLTKR----NSINS-RSESRKENIITKLFRCTEDRSASLKKALLIINTKPDGPITREEFRYILNCVAIKLSDSEFKELMQILDPGDTGVVNTSMFIDLIE--ENCRMRK----TSPCTDAKTPFLLAWDSVEEIVHDTIARNLQAFYNMLRSYDLGDTGLIGRNNFKKIMHVFCPFLTNAHFIKLCS--KIQDIGSGRILYKKLLACIGIDGPPTVSPVLVPKDQLLSEHLQKDEQQQPDLSERTKPTEDKTTLTKKMTT-EEVIEKFRKYIQQQDPAFKKRFLDFS-KEPNGKINVHDFRKILEDTGMPMDDDQYALLTTKIGFEKEGMSYLDF---AAGFEDPPMRGLETTPPQ-PPTPSKSYVN------SHLITAEECLKLFPRRLKESFRDPYSAFFKTDVDRDGIINMHDLHRLLLHLLLNLKDDEFERFLGLLGLRLSVTLNFREFQNLCEKRPWRTD-----EAPQRLIRPKQKVADSELACEQAHQYLVTKAKNRWSDLSKNFLETDNEGNGILRRRDIKNALYGFDIPLTPREFEKLWARYNTEGKGHITYQEFLQKLGINYSPA---VHQPCAEDYFNFMGHFTKPQQLQEEMKELQQSTEKAMA-------------ARDKLMDLHQDISKAFTKIDKSKT--NYISICKMQKVLEECGCSLTEGELTHLLNSWGVSRHENAINYLDFLRAVENSKSTGAQPK-----------EKEESMPISFA-TLNPQEVVRKIQEVVESSQLALSTAFSALDKEDTGFVKATEFGQVLKDFCYKLMDNQYHYFLRKLRIHLTPY------INWKYFLQNFSCFLEEVRI 991 HSP 2 Score: 78.9518 bits (193), Expect = 3.216e-13 Identity = 142/691 (20.55%), Postives = 271/691 (39.22%), Query Frame = 3
Query: 660 DDIIPILQMQISRNPNGIMKAFKLFDFNSDYCIQRHRLKKVMENFCCPLTEQQFDKLVARFSQSQNGNVNYMEFMKGMGI--PIDNQGTIQTKN---SEETINIDTRNTKGSRIFEAALIENGLLGSNELEQLLVIFRKKIEDQFSVLRKTFLVMDPNAEGFVTLEILYKILNQFAFPMSLNVFNQIMDKCAFKATGKIIWLSFLQKFQDPQSKHLNGSTIAIKSDHKCNPIQGVNSK-PNIDDLLELLSNHISNNYSSFRDAFLQINQTHKGKIPKSEFLKILNRFQFYISADQLSIILKFLDPHNEGYISYHHFLQLFEQKENLSAHKWLN--SNHKVVEPRK---DLQLSSETIKSTISDNIGNKWSNVVHEFNLVDLNRTGYISVEDLKKIFKKLFGY---LKDDDIDRLVAPLTLKDKKGRINYLELFNLLNVDTSPSDLEGLSTQIAE------SNDAQLKERHENHIEKMESIEKLGEARLNLMSPEELIVRIKDRIVQNRLNYHELYKKYECNDKKGGINKSQLRKMLTQFGYTLTNEQFSKISEILNFPTSSK--SRIDFNGFLAIFADNRESSNDLMTDVCFLPKSNHHVNDIRGDDNRNMNCQEVEDLLRQKIVNNFPDLMIAFHKFDRSNKGYLVKSDFQNFLLGNLIMIENDQFDLLCEKLGVGEKSKLTYNDFLNRF 2666
D + I+ I+RN + +D I R+ KK+M FC LT F KL ++ +G + Y + + +GI P + K+ SE + + S + + L E+++ FRK I+ Q +K FL G + + KIL PM + + + K F+ G + +L F F+DP + L + + K VNS ++ L+L + ++ AF + + G I + ++L + D+ L L +++ F L E++ W + +++ P++ D +L+ E + N+WS++ F D G + D+K L+G+ L + ++L A + KG I Y E L ++ SP+ + AE + + ++ E E +S EK AR LM + I + +I +++ NY I+ +++K+L + G +LT +++ +LN S+ + I++ FL +++ + PK I +N QEV +++ + ++ L AF D+ + G++ ++F L + ++Q+ KL + + + FL F
Sbjct: 340 DSVEEIVHDTIARNLQAFYNMLRSYDLGDTGLIGRNNFKKIMHVFCPFLTNAHFIKLCSKIQDIGSGRILYKKLLACIGIDGPPTVSPVLVPKDQLLSEHLQKDEQQQPDLSERTKPTEDKTTLTKKMTTEEVIEKFRKYIQQQDPAFKKRFLDFSKEPNGKINVHDFRKILEDTGMPMDDDQYALLTTKIGFEKEG-MSYLDFAAGFEDPPMRGLETTPPQPPTPSK----SYVNSHLITAEECLKLFPRRLKESFRDPYSAFFKTDVDRDGIINMHDLHRLLLHLLLNLKDDEFERFLGLLGLRLSVTLNFREFQNLCEKR------PWRTDEAPQRLIRPKQKVADSELACEQAHQYLVTKAKNRWSDLSKNFLETDNEGNGILRRRDIK---NALYGFDIPLTPREFEKLWARYN-TEGKGHITYQEFLQKLGINYSPA----VHQPCAEDYFNFMGHFTKPQQLQEEMKELQQSTEKAMAARDKLMDLHQDISKAFTKIDKSKTNY---------------ISICKMQKVLEECGCSLTE---GELTHLLNSWGVSRHENAINYLDFLRAVENSKSTGAQ--------PKEKEESMPI---SFATLNPQEVVRKIQEVVESSQLALSTAFSALDKEDTGFVKATEFGQVLKDFCYKLMDNQYHYFLRKLRIHLTPYINWKYFLQNF 982 HSP 3 Score: 76.2554 bits (186), Expect = 2.336e-12 Identity = 79/342 (23.10%), Postives = 154/342 (45.03%), Query Frame = 3
Query: 195 ISEINPIVKSQLLKNHKEILKALQTFDIECSGLVTKGELRRVLDSYCLPMTTDQYEQFLSHNSINYSKGSINYKELIDKIYDKTSAVGHFKKNIKLPQLQRSATTICLQNKHFTLPTTRRSSGKISRSQLFXXXXXXXXXXXXXXXXWNYNAYTIDDIIPILQMQISRNPNGIMKAFKLFDFNSDYCIQRHRLKKVMENFCCPLTEQQFDKLVARFSQSQNGNVNYMEFMKGM----GIPIDNQGTIQTKNSEETINIDTRNTKGSRIFEAALIENGLLGSNELEQLLVIFRKKIEDQFSVLRKTFLVMDPNAEGFVTLEILYKILNQFAFPMSLNVFNQIM 1208
+ E+ V ++ KN K ++KA + D+ +GLV ELRRVL+++CL + ++YE+F H +I ++ +++Y ++ K ++ + N++ C+ N+ +L + + K R L S S W Y++DDI +++S++ I KA D + + LK V++ F + + F +L+ RF +N+ +F+ G+ + N+G + +NS IN + + K E ++ + ED+ + L+K L+++ +G +T E ILN A +S + F ++M
Sbjct: 7 LRELEIQVGEKVFKNIKTVMKAFKLIDVNKTGLVRPQELRRVLETFCLKLRDEEYEKFSKHYNI-HNDTAVDY-----NVFLKNLSINN-DLNLRY----------CMGNQEVSLENQQARNSKKER----------LLGSASSEDIW--RNYSLDDIERNFCLELSKSYEKIEKALSAGDPCKGGYVSFNYLKIVLDTFIYQIPRRIFIQLMKRFGLKATTKINWKQFLTSFHEPQGLQVSNKGPLTKRNS---INSRSESRK--------------------ENIITKLFRCTEDRSASLKKALLIINTKPDGPITREEFRYILNCVAIKLSDSEFKELM 296 HSP 4 Score: 60.077 bits (144), Expect = 1.897e-7 Identity = 79/428 (18.46%), Postives = 176/428 (41.12%), Query Frame = 3
Query: 2787 EKIYQYFHEISPTFIALVTSGTGFIRFRNLQHLLSRLQISMSKSEMKKLWKRYDTNNRNKLDYHDFLYNLGLLEKFGCGDLMGPSAAIIQNSH------NALVDHLVNQETKHQQSVDNQAVLGTHLHVEHLFEMVKRHLRVAYSDSALAFKAVANKKTLSGFFTINDLNKLLSQLHMFVTNDKFVQLAARMGFEGPDDSLSYEKFCCIFQDSLNIDGQEKTVGSIRINQDLENPSKIGIEEAGNLNSTQ------IASIIGKRIVDQYSEIEKTFLVMDPENSGYVSMNEFRNLLVGFCLSLTDKQFNKYRNSL---STYIVKSANTDDKIDYRYFMKGFNQKLNNLDIQXXXXXXXXXXXXXTYQQIQEHINESIKVRPKLFLNSFKSKDYASLNFISKSDFREIIERFLFTVPDSHFEKFWSNLK 4025
EK+++ + F + + TG +R + L+ +L + + E +K K Y+ +N +DY+ FL NL + MG ++N L+ +++ S+D+ + E+ K + ++ + AL+ A G+ + N L +L + F+QL R G + +++++F F + Q L+ +K + + ++NS I + + + D+ + ++K L+++ + G ++ EFR +L + L+D +F + L T +V ++ D I+ M+ ++ A + + ++E ++++I + F N +S D I +++F++I+ F + ++HF K S ++
Sbjct: 16 EKVFKNIKTVMKAFKLIDVNKTGLVRPQELRRVLETFCLKLRDEEYEKFSKHYNIHNDTAVDYNVFLKNLSINNDLNLRYCMGNQEVSLENQQARNSKKERLLGSASSEDIWRNYSLDD-------IERNFCLELSKSYEKI---EKALS----AGDPCKGGYVSFNYLKIVLDTFIYQIPRRIFIQLMKRFGLKA-TTKINWKQFLTSFHEP----------------QGLQVSNKGPLTKRNSINSRSESRKENIITKLFRCTEDRSASLKKALLIINTKPDGPITREEFRYILNCVAIKLSDSEFKELMQILDPGDTGVVNTSMFIDLIEENCRMR-----------KTSPCTDAKTPFLLAWDSVEEIVHDTIARNLQAFYNMLRSYDLGDTGLIGRNNFKKIMHVFCPFLTNAHFIKLCSKIQ 401
BLAST of EF-hand calcium-binding domain-containing protein 6 vs. UniProt/SwissProt
Match: sp|Q6P1E8|EFCB6_MOUSE (EF-hand calcium-binding domain-containing protein 6 OS=Mus musculus OX=10090 GN=Efcab6 PE=1 SV=2) HSP 1 Score: 193.356 bits (490), Expect = 4.450e-48 Identity = 222/1143 (19.42%), Postives = 480/1143 (41.99%), Query Frame = 3
Query: 678 LQMQISRNPNGIMKAFKLFDFNSDYCIQRHRLKKVMENFCCPLTEQQFDKLVARFSQSQNGNVNYMEFMKGMGIPIDNQGTIQTKNSEETINIDTRNTKGSRIFEAALIENGLLGSNELEQLLVIFRKKIEDQFSVLRKTFLVMDPNAEGFVTLEILYKILNQFAFPMSLNVFNQIMDKCAFKATGKIIWLSFLQKFQDPQSKHLNGSTIAIKSDHKCNPIQGVNSKPNIDDLLELLSNHISNNYSSFRDAFLQINQTHKGKIPKSEFLKILNRFQFYISADQLSIILKFLDPHNEGYISYHHFLQLFEQKENLSAHKWLNSNHKVVEPRKDLQLSSETIKSTISDNIGNKWSNVVHEFNLVDLNRTGYISVEDLKKIFKKLFGYLKDDDIDRLVAPLTLKDKKGRINYLELFNLLNVDTSPSDLEGLSTQIAESNDAQLKERHENHIEKMESIEKLGEARLNLMSPEELIVRIKDRIVQN----RLNYHELYKKYECNDKKGGINKSQLRKMLTQFGYTLTNEQFSKISEILNFPTSSKSRIDFNGFLAIFADNRESSNDLMTDVCFLPKSNHHVNDIRGDDNRNMNCQEVEDLLRQKIVNNFPDLMIAFHKFDRSNKGYLVKSDFQ---NFLLGNLIMIENDQFDLLCEKLGVGEKSKLTYNDFLNRFQIRDDMVD--GHKWLDSKHRFNDPSTNAVPDMSAEDVYMLLPEKIYQYFHEISPTFIALVTSGTGFIRFRNLQHLLSRLQISMSKSEMKKLWKRYDTNNRNKLDYHDFLYNLGLLEKFGCGDLMGPSAAIIQNSHNALVDHLVNQETKHQQSVDNQAVLGTHLHVEHLFEMVKRH-LRVAYSDSALAFKAVANKKTLSGFFTINDLNKLLSQLHMFVTNDKFVQLAARMGFEGPDDSLSYEKFCCIFQDSLNIDGQEKTVGSIRINQDLENPSKIGIEEAGNLNSTQIASIIGKRIVDQYSEIEKTFLVMDPENSGYVSMNEFRNLLVGFCLSLTDKQFNKYRNSLSTYIVKSANTDDKIDYRYFMKGF----NQKLNNLDIQXXXXXXXXXXXXXTYQQIQEHINESIKVRPKLFLNSFKSKDYASLNFISKSDFREIIERFLFTVPDSHFEKFWSNLKKNEFDNVNYGEFV 4064
L +I RN I K F++ D N+ +Q L++V+E FC + + ++K + +++ + V+Y F+K + + D N+ E + G R+ + E+ + + L+ L F ++ + + K DP+ G+++L L +L+ F + + +F Q++ + K + KI W FL + Q ++ T+ +K ++ +++++ L + + Y++ + L I+ T G I E ILN ++ + S + + DP G + + L + + ++ K S + + L ++ ++++ + D+I DL TG I + +K+ + YL ++ + R + + GRI Y + + V P S ++ S+ Q++E + S EK E +N M+ EE+I +K RI Q R + + K+ + IN+ + RK+L + G + + Q++ ++ L F ++ + + F F D +++ + +P + + D++ ++ +E + +K+ +F D+ AF + D G + DF +L N++ +E ++F L LG+ L + +F N + R D + + K + D +++ E + L K + ++S FI G G +R R++++ L I ++ E +KLW+ YDT R + Y +FL+ LG+ + + + + + TK +Q ++ L ++ +R L Y + + AF A+ K ++ + + K+L + + ++ + L + ++ + +F + S + K ++ +P I + ++ + + + + + F +D E++G+V EF ++L C LTD Q++ + L ++ + I ++YF++ F ++ ++ A P + ++ I + +++ + F++ D N +S+ +FR I R + + D F++ WS L N + Y +F+
Sbjct: 191 LTEKIFRNMRSIRKVFQVMDVNNTGLVQPQELRRVLETFCLRMQDGDYEKFLEQYNIDKTTAVDYNAFLKNLSVKNDASFKYLLSNAAELSRETQQGKNGKRLLDTGSSED-VWKNYSLDDLEKTFCQEFSKSYEKIEKALSAGDPSKGGYISLNYLKVVLDTFIYRLPRRIFIQLIRRFGLKTSTKINWKQFLTAIYERQKLEVS-KTLPLKKRSSTE----ARNRSRKENIIKKLFKYSEDRYTALKKTLLIISSTPSGHITWEELRHILNCMVAKLNDSEFSELKQTFDPEGTGAVRVNSLLDVLD--DSTKVRKMSPS----TDTKTPLPVAWDSVEELVLDSITRNLQAFYSMLQSYDLRDTGTIGKNNFRKVMRVFCPYLSNEHLVRFSSKFQ-EAGSGRILYKKFLLSIGVGIPPPTPPLSSPKVQLSDQFQIEEPGQQDERTQPSGEKTSE--INNMTKEEVIDGLKHRIQQKDPVFRKQFLSISKEPDVK-----INQEEFRKVLERSGMPMNDCQYAMLASKLGF--KNEEGMSYQDFTMGFEDC------MLSGLETVPLQSRTASRTNMDEH-FISAEECLRIFPKKLKESFRDVYSAFFRIDLDRDGIISMHDFHRLLQYLQLNMVDLEFERFLSL---LGLRLSVTLNFREFQNLCEKRPWKSDEAPQRLIRCKQKVADS------ELACEQAHQYLIMKAKTRWADLSKNFIETDNEGNGILRRRDIKNSLYGFDIPLTPREFEKLWQNYDTEGRGYITYQEFLHRLGIR-----------YSPKVHRPYKEDYFNFLGHFTKPKQ---------VQEEIQELQQISEREKLMNHYEEISKAFNAMEKSKPVA----LCRVQKVLQECGCPLKEEELISLLKSLDVSVHNNHIDPVEFLRALEISWASKARPK-------EKEESSPPPISF---SKVTPDEVIKTMQEVVESSQPALVEAFSALDKEDTGFVKAMEFGDVLRSVCQKLTDNQYHYFLRRLRLHLTPN------IHWKYFLENFSTFQDETADDWAENMPKAPPPMSPKETAHRDIVARVQKAVASHYHTIVQEFENFDTLKSNTVSRDEFRSICTRHIQILTDEQFDRLWSELPVNAKGRLKYQDFL 1255 HSP 2 Score: 114.775 bits (286), Expect = 4.889e-24 Identity = 166/788 (21.07%), Postives = 302/788 (38.32%), Query Frame = 3
Query: 663 DIIPILQMQISRNPNGIMKAFKLFDFNSDYCIQRHRLKKVMENFCCPLTEQQFDKLVARFSQSQNGNVNYMEFMKGMGIPID-NQGTIQTKNSEETINIDTRNTKGSRIFEAALIENGLLGSNELEQLLVIFRKKIEDQFSVLRKTFLVMDPNAEGFVTLEILYKILNQFAFPMSLNVFNQIMDKCAFKATGKIIWLSFLQKFQ---DPQSKHLNGSTIAIKSDHKCNPIQGVNSKP--------------NIDDLLELLSNHISNNYSSFRDAFLQINQTHKGKIPKSEFLKILNRFQFYISADQLSIILKFLDPHNEGYISYHHFLQLFEQKENLSAHKWLNSNHKVVEPRKDLQLSSETIKSTISDNIGNKWSNVVHEFNLVDLNRTGYISVEDLKKIFKKLFGYLKDDDIDRLVAPLTLKDKKGRINYLELFNLLNVDTSPSDLEGLSTQIAESNDAQLKERHENHIEKME-SIEKLGEARLNLMSPEELIVRIKDRIVQNRLNYHELYKKYECNDKKGGINKSQLRKMLTQFGYTLTNEQFSKISEILNFPTSSKSRIDFNGFLAIFADNRESSNDLMTDVCFLPKSNHHVNDIRGDDNR------------NMNCQEVEDLLRQKIVNNFPDLMIAFHKFDRSNKGYLVKSDFQNFLLGNLIMIENDQFDLLCEKLGVGEKSKLTYNDFLNRFQIRDDMVDGHKWLDSKHRFNDPSTNAVPDMSAEDVYMLLPEKIYQYFHEISPTFIALVTSGTGFIRFRNLQHLLSRLQISMSKSEMKKL 2933
DI IL +IS + I K F++ D N + + + LK+V+ F PLT+ QF L+A+ S GNV Y+EF+ G ID N I+ N E +D R T + E L E IFR +RK F VMD N G V + L ++L F M + + +++ T + + +FL+ D K+L + + + + QG N K ++DDL + S +Y A + + G I + +L+ F + + +++ I++ FL +++ L K L + ++ IK + ++++ + ++ +G+I+ E+L+ I + L D + L + G + L ++L+ T + KM S + + S EEL++ D I +N ++ + + Y+ D G I K+ RK++ F L+NE + S F + RI + FL ++ + + D R NM +EV D L+ +I P F + + + +F+ L + + + + Q+ +L KLG + ++Y DF F+ D M+ G + + + R + +SAE+ + P+K+ + F ++ F + G I + LL LQ++M E ++
Sbjct: 85 DIERILAQKISSRRDDIKKVFQILDRNHNQMVTKGDLKRVITAFLIPLTKDQFQDLLAQIPISSLGNVPYLEFLSRFGGGIDININGIKRVNENE---VDNRRT----VKEVQLTEK-------------IFR-----NMRSIRKVFQVMDVNNTGLVQPQELRRVLETFCLRMQDGDYEKFLEQYNIDKTTAVDYNAFLKNLSVKNDASFKYLLSNAAELSRETQ----QGKNGKRLLDTGSSEDVWKNYSLDDLEKTFCQEFSKSYEKIEKALSAGDPSKGGYISLNYLKVVLDTFIYRLPRRIFIQLIRRFGLKTSTKINWKQFLTAIYERQKLEVSKTLPLKKRSSTEARNRSRKENIIKKLFKYS-EDRYTALKKTLLIISSTPSGHITWEELRHILNCMVAKLNDSEFSELKQTFD-PEGTGAVRVNSLLDVLDDSTK--------------------------VRKMSPSTDTKTPLPVAWDSVEELVL---DSITRNLQAFYSMLQSYDLRD-TGTIGKNNFRKVMRVFCPYLSNEHLVRFSS--KFQEAGSGRILYKKFLLSIGVGIPPPTPPLSSPKVQLSDQFQIEEPGQQDERTQPSGEKTSEINNMTKEEVIDGLKHRIQQKDPVFRKQFLSISKEPDVKINQEEFRKVLERSGMPMNDCQYAMLASKLGFKNEEGMSYQDFTMGFE--DCMLSGLETVPLQSRTASRTNMDEHFISAEECLRIFPKKLKESFRDVYSAFFRIDLDRDGIISMHDFHRLLQYLQLNMVDLEFERF 807 HSP 3 Score: 90.1225 bits (222), Expect = 1.654e-16 Identity = 188/1081 (17.39%), Postives = 410/1081 (37.93%), Query Frame = 3
Query: 690 ISRNPNGIMKAFKLFDFNSDYCIQRHRLKKVMENFCCPLTEQQFDKLVARFSQSQNGNVNYMEFMKGMGIPIDNQGTIQTKNS---EETINIDTRNTKGSRIFEAALIENGLLGSNELEQLLVIFRKKIEDQFSVLRKTFLVMDPNAEGFVTLEILYKILNQFAFPMSLNVFNQIMDKCAFKATGKIIWLSFLQKFQDPQSKHLNGSTIAIKSDHKCNPIQGVNSKPNIDDLLELLSNHISNNYSSFRDAFLQINQTHKGKIPKSEFLKILNRFQFYISADQLSIILKFLDPHNEGYISYHHFLQLFEQ---KENLSAHKWLNSNHKVVEPRKDLQLSSETIKSTISDNIGNKWSNVVHEFNLVDLNRTGYISVEDLKKIFKKLFGYLKDDDIDRLVAPLTLK---------DKKGR--INYLELFNLLNVDTSP-------SDLEGLSTQIAESNDAQLKERHENHIEKMESIEKLGEARLNLMSPEELIVRIKDRIVQNRLNYHELYKKYECNDKKGGINKSQLRKMLTQFGYTLTNEQFSKISEILNFPTSSKSRIDFNGFL-------AIFADNRESSNDLMTDVCFLPKSNHHVNDIRGDDNRNMNCQEVEDLLRQKIVNNFPDLMIAFHKFDRSNKGYLVKSDFQNFLLGNLIMIENDQFDLLCEKLGVGEKSKLTYNDFLNRFQ-IRDDMVDGHKWLDSKHRFNDPSTNAVPDMSAEDVYMLLPEKIYQYFHEISPTFIALVTSGTGFIRFRNLQHLLSRLQISMSKSEMKKLWKRYDTNNRNKLDYHDFLYNLGLLE----KFGCGDLMGPSAAIIQNSHNALVDHLVNQETKHQQSVDNQAVLGTHLH------------VEHLFEMVKRHLRVAYSDSALAFKAVANKKTLSGFFTINDLNKLLSQLHMFVTNDKFVQLAARMGFEGPDDSLSYEKFCCIFQDSLNIDGQEKTVGSIRINQDLENPSKIGIEEAGNLNSTQIASI--IGKRIVDQYSEIEKTFLVMDPENSGYVSMNEFRNLLVGFCLSLTDKQF----NKYRNSLSTYIVKSANTDDKIDYRYFMKGFNQ 3770
I+RN + +D I ++ +KVM FC L+ + + ++F ++ +G + Y +F+ +G+ I + + I+ + R + + + + + E+++ + +I+ + V RK FL + + + E K+L + PM+ + + K FK + + F F+D L + ++ + N + S ++ L + + ++ AF +I+ G I +F ++L Q + + L L +++ F L E+ K + + + + KV D +L+ E + +W+++ F D G + D+K L+G+ DI PLT + D +GR I Y E + L + SP D + Q E+++ ++++ E R LM+ +Y E+ K + +K + +++K+L + G L E+ + + L+ + + ID FL A A +E + F + EV +++ + ++ P L+ AF D+ + G++ +F + L + ++Q+ +L + + + FL F +D+ D W ++ + P + + + D+ + + + ++H I F T + + + + +R ++ + +LW N + +L Y DFL L + GD + + Q +A + + D++ L + H E + +++ ++ + + K K G + + L+ + + ++ ++ QL + + + +Y C F S + + K +R ++N K ++EAG + +++ I +IV + + ++F D +G +S+ +FR +L + ++L++++F Y SLS+ KI Y F++ F Q
Sbjct: 526 ITRNLQAFYSMLQSYDLRDTGTIGKNNFRKVMRVFCPYLSNEHLVRFSSKFQEAGSGRILYKKFLLSIGVGIPPPTPPLSSPKVQLSDQFQIEEPGQQDERT-QPSGEKTSEINNMTKEEVIDGLKHRIQQKDPVFRKQFLSISKEPDVKINQEEFRKVLERSGMPMNDCQYAMLASKLGFKNEEGMSYQDFTMGFEDCMLSGLETVPLQSRTASRTNMDEHFIS---AEECLRIFPKKLKESFRDVYSAFFRIDLDRDGIISMHDFHRLLQYLQLNMVDLEFERFLSLLGLRLSVTLNFREFQNLCEKRPWKSDEAPQRLIRCKQKVA----DSELACEQAHQYLIMKAKTRWADLSKNFIETDNEGNGILRRRDIK---NSLYGF----DI-----PLTPREFEKLWQNYDTEGRGYITYQEFLHRLGIRYSPKVHRPYKEDYFNFLGHFTKPKQVQ---------EEIQELQQISE-REKLMN-----------------HYEEISKAFNAMEKSKPVALCRVQKVLQECGCPLKEEELISLLKSLDVSVHN-NHIDPVEFLRALEISWASKARPKEKEESSPPPISF----------------SKVTPDEVIKTMQEVVESSQPALVEAFSALDKEDTGFVKAMEFGDVLRSVCQKLTDNQYHYFLRRLRLHLTPNIHWKYFLENFSTFQDETADD--WAENMPK--APPPMSPKETAHRDIVARVQKAVASHYHTIVQEFENFDTLKSNTVSRDEFRSICTRHIQILTDEQFDRLWSELPVNAKGRLKYQDFLSKLSIERVPSPPMAAGD--SGESTMAQRGSSAPEFSQGTRSNLYSPPRDSRVGLKSRSHPCTPVGTPPLQNCEPIESRLRKQIQGCWRELLRECKEKDTDK--QGTISAAEFLALVEKFKLDISREESQQLIVKYDLKN-NGKFAY----CDFIQSCVLLLKAKETSLMR-RMRIQNADK--MKEAGMETPSFYSALLRIQPKIVHCWRPMRRSFKTYDKNGTGLLSVADFRKVLRQYSINLSEEEFFHVLEYYDKSLSS----------KISYNDFLRAFLQ 1516 HSP 4 Score: 85.5001 bits (210), Expect = 3.760e-15 Identity = 106/554 (19.13%), Postives = 231/554 (41.70%), Query Frame = 3
Query: 2436 EVEDLLRQKIVNNFPDLMIAFHKFDRSNKGYLVKSDFQNFLLGNLIMIENDQFDLLCEKLGVGEKSKLTYNDFLNRFQIRDDM-VDGHKWLDSKHRFNDPSTNAVPDMSAEDVYMLLPEKIYQYFHEISPTFIALVTSGTGFIRFRNLQHLLSRLQISMSKSEMKKLWKRYDTNNRNKLDYHDFLYNLGLLEKFGCGDLMGPSAAIIQNSHNA-----LVDHLVNQETKHQQSVDNQAVLGTHLHVEHLF--EMVKRHLRVAYSDSALAFKAVANKKTLSGFFTINDLNKLLSQLHMFVTNDKFVQLAARMGFEGPDDSLSYEKFCCIFQDSLNIDGQEKTVGSIRINQDLENPSKIGIEEAGNLNSTQIASIIGKRIVDQYSEIEKTFLVMDPENSGYVSMNEFRNLLVGFCLSLTDKQFNKYRNSL---STYIVKSANTDDKIDYRYFMKGFNQKLNNLDIQXXXXXXXXXXXXXTYQQIQEHINESIKVRPKLFLNSFKSKDYASLNFISKSDFREIIERFLFTVPDSHFEKFWSNLKKNEFDNVNYGEFV 4064
++E +L QKI + D+ F DR++ + K D + + LI + DQF L ++ + + Y +FL+RF D+ ++G K ++ N + V L EKI++ I F + + TG ++ + L+ +L + M + +K ++Y+ + +DY+ FL NL + L+ +A + + + L+D +++ S+D+ +E F E K + ++ + AL+ A + G+ ++N L +L + F+QL R G + +++++F + ++ +++ L + E I + K D+Y+ ++KT L++ SG+++ E R++L L D +F++ + + T V+ + D +D ++ + + + + ++E + +SI + F + +S D I K++FR+++ F + + H +F S ++ + Y +F+
Sbjct: 85 DIERILAQKISSRRDDIKKVFQILDRNHNQMVTKGDLKRVITAFLIPLTKDQFQDLLAQIPISSLGNVPYLEFLSRFGGGIDININGIKRVNENEVDNRRTVKEVQ----------LTEKIFRNMRSIRKVFQVMDVNNTGLVQPQELRRVLETFCLRMQDGDYEKFLEQYNIDKTTAVDYNAFLKNLSVKNDASFKYLLSNAAELSRETQQGKNGKRLLDTGSSEDVWKNYSLDD---------LEKTFCQEFSKSYEKI---EKALS----AGDPSKGGYISLNYLKVVLDTFIYRLPRRIFIQLIRRFGLKT-STKINWKQFLTAIYERQKLE----------VSKTLPLKKRSSTEARNRSRKENIIKKLFKYSEDRYTALKKTLLIISSTPSGHITWEELRHILNCMVAKLNDSEFSELKQTFDPEGTGAVRVNSLLDVLDDSTKVRKMSPSTDT-----------KTPLPVAWDSVEELVLDSITRNLQAFYSMLQSYDLRDTGTIGKNNFRKVMRVFCPYLSNEHLVRFSSKFQEAGSGRILYKKFL 590 HSP 5 Score: 75.485 bits (184), Expect = 4.251e-12 Identity = 180/1085 (16.59%), Postives = 412/1085 (37.97%), Query Frame = 3
Query: 204 INPIVKSQLLKNHKEILKALQTFDIECSGLVTKGELRRVLDSYCLPMTTDQYEQFLSHNSINYSKGSINYKELIDKIYDKTSAVGHFKKNIKLPQLQRSATTICLQNKHFTLPTTRRSSGKISRSQLFXXXXXXXXXXXXXXXXWNYNAYTIDDIIPILQMQISRNPNGIMKAFKLFDFNSDYCIQRHRLKKVMENFCCPLTEQQFDKLVARFSQSQNGNVNYMEFMKGMGIPIDNQGTIQTKNSEETINIDTRNTKGSRIFEAALIENGLLGSNELEQLLVIFRKKIEDQFSVLRKTFLVMDPNAEGFVTLEILYKILNQFAFPMSLNVFNQIMDKCAFKATGKIIWLSFLQKFQDPQSKHLNGSTIAIKSDHKCNPIQGVNSKPNIDDLLELLSNHISNNYSSFRDAFLQINQTHKGKIPKSEFLKILNRFQFYISADQLSIILKFLDPHNEGYISYHHFLQLFEQKENLSAHK-----WLNSNHKVVEPRKDLQLSSETIKSTISDNIGNKWSNVVHEFNLVDLNRTGYISVEDLKKIFKKLFGYLKDDDIDRLVAPLTLKDKKGRINYLELFNLLNVDTSPSDLEGLSTQIAESNDAQLKERHENHIEKMESIEKLGEARLNLMSPEELIVRIKDRIVQNRLNYHELYKKYECNDKKGGINKSQLRKMLTQFGYTLTNEQFSKISEILNFPTSSKSRIDFNGFLAIFAD-NRESSNDLMTDVCFLPKSNHHVNDIRGDDNRNMNCQEVEDLLRQKIVNNFPDLMIAFHKFDRSNKGYLVKSDFQNFLLGNLIMIENDQFDLLCEKLGVGEKSKLTYNDFLNRFQI--------------------------------RDDMV----DGHKWLDSKHRFNDPSTNAVPDMSAEDVYMLLPEKIYQYFHEISPTFIALVTSGTGFIRFRNLQHLLSRLQISMSKSEMKKLWKRYDTNNRNKLDYHDFLYNLGLLEKFGCGDLMGPSAAIIQNSHNALVDHLVNQETKHQQSVDNQAVLGTHLHVEHLFEMVKRHLRVAYSDSALAFKAVANKKTLSGFFTINDLNKLLSQLHMFVTNDKFVQL 3332
+ +V + +N + LQ++D+ +G + K R+V+ +C P ++++ S G I YK+ + I + K+ + Q T P+ ++S N T +++I L+ +I + K F D I + +KV+E P+ + Q+ L ++ ++Y +F G + + ET+ + +R + + E + E+ L IF KK+++ F + F +D + +G +++ +++L M F + + + + + L+F ++FQ+ K S A + +C + +S+ + + L ++ F++ + G + + + L F ++ + + + D GYI+Y FL + + H+ + N +P++ + E + + + + N + + FN ++ ++ +++ ++K+ ++ LK++++ L+ L + I+ +E L +I+ ++ A+ KE+ E+ + + ++P+E+I +++ + ++ E + + D G + + +L LT+ Q+ L + I + FL F+ E+++D + +PK+ ++ + +++ +++ + +++ ++ F FD + + +F++ ++ ++ ++QFD L +L V K +L Y DFL++ I R ++ D L S+ P P + E + L ++I + E+ T G I L+ + ++ +S+ E ++L +YD N K Y DF+ + LL K LM IQN+ +E + A+L + H + ++R +FK K +G ++ D K+L Q + ++ ++F +
Sbjct: 518 VEELVLDSITRNLQAFYSMLQSYDLRDTGTIGKNNFRKVMRVFC-PYLSNEHLVRFSSKFQEAGSGRILYKKFLLSIGVGIPPPTPPLSSPKVQLSDQFQIEEPGQQDERTQPSGEKTS--------------------------EINNMTKEEVIDGLKHRIQQKDPVFRKQFLSISKEPDVKINQEEFRKVLERSGMPMNDCQYAMLASKLGFKNEEGMSYQDFTMGFEDCM--------LSGLETVPLQSRTASRTNMDEHFI---------SAEECLRIFPKKLKESFRDVYSAFFRIDLDRDGIISMHDFHRLLQYLQLNMVDLEFERFLSLLGLRLS---VTLNF-REFQNLCEKRPWKSDEAPQRLIRCKQ-KVADSELACEQAHQYLIMKAKTRWADLSKNFIETDNEGNGILRRRDIKNSLYGFDIPLTPREFEKLWQNYDTEGRGYITYQEFLHRLGIRYSPKVHRPYKEDYFNFLGHFTKPKQVQEEIQELQQISEREKLMNHYEEISKAFNAME--KSKPVALCRVQKVLQECGCPLKEEELISLLKSLDVSVHNNHIDPVEFLRAL--------------EISWASKARPKEKEESSPPPIS---------FSKVTPDEVIKTMQEVVESSQPALVEAFSALDKEDT-GFVKAMEFGDVLRSVCQKLTDNQYHYFLRRLRLHLTPN--IHWKYFLENFSTFQDETADDWAEN---MPKAPPPMSP------KETAHRDIVARVQKAVASHYHTIVQEFENFDTLKSNTVSRDEFRSICTRHIQILTDEQFDRLWSELPVNAKGRLKYQDFLSKLSIERVPSPPMAAGDSGESTMAQRGSSAPEFSQGTRSNLYSPPRDSRVGLKSRSHPCTP-VGTPPLQNCEPIESRLRKQIQGCWRELLRECKEKDTDKQGTISAAEFLALVEKFKLDISREESQQLIVKYDLKNNGKFAYCDFIQSCVLLLKAKETSLM--RRMRIQNADKM-------KEAGMETPSFYSALLRIQPKIVHCWRPMRR-----------SFKTY--DKNGTGLLSVADFRKVLRQYSINLSEEEFFHV 1493 HSP 6 Score: 65.4698 bits (158), Expect = 4.294e-9 Identity = 49/213 (23.00%), Postives = 97/213 (45.54%), Query Frame = 3
Query: 4155 IETDEMDTDESIRKNLERLLNRNWQSIFNKCKAIDQAINECQTAEGSIPIDDFVYILSEYDDSLS-AENLAAINLYKTCKNGRFIKYGDLIRSIVLSSRGSLISNENVTEVQK--KFLNVGIKTTNSDNSKDYFPQLFKLRRQILENWKDLRKNFLQSDKKQNGAVSGNIFMTIMKTVCGQLSADLFYHLMKIYDQDGTGNFHYKRFIEDFLK 4784
+ T + E I L + + W+ + +CK D +G+I +F+ ++ ++ +S E+ I Y NG+F Y D I+S VL + S +Q K G++T + ++ L +++ +I+ W+ +R++F DK G +S F +++ LS + F+H+++ YD+ + Y F+ FL+
Sbjct: 1317 VGTPPLQNCEPIESRLRKQIQGCWRELLRECKEKDT------DKQGTISAAEFLALVEKFKLDISREESQQLIVKYDLKNNGKFA-YCDFIQSCVLLLKAKETSLMRRMRIQNADKMKEAGMETPS------FYSALLRIQPKIVHCWRPMRRSFKTYDKNGTGLLSVADFRKVLRQYSINLSEEEFFHVLEYYDKSLSSKISYNDFLRAFLQ 1516
BLAST of EF-hand calcium-binding domain-containing protein 6 vs. UniProt/SwissProt
Match: sp|Q4R8T1|EFCB6_MACFA (EF-hand calcium-binding domain-containing protein 6 (Fragment) OS=Macaca fascicularis OX=9541 GN=EFCAB6 PE=2 SV=2) HSP 1 Score: 105.145 bits (261), Expect = 1.571e-21 Identity = 135/670 (20.15%), Postives = 283/670 (42.24%), Query Frame = 3
Query: 756 IQRHRLKKVMENFCCPLTEQQFDKLVARFSQSQNGNVNYMEFMKGMGIPIDNQGTIQTKNSEETINIDTRNTKGSRIFEAALIENGLLGSNELEQL---LVIFRKKIEDQFSVLRKTFLVMDPNAEGFVTLEILYKILNQFAFPMSLNVFNQIMDKCAF-KATGKIIWLSFLQKFQDPQSKHLNGSTIAIKSDHKCNPIQGVNSKPNIDDLLELLSNHISNNYSSFRDAFLQINQTHKGKIPKSEFLKILNRFQFYISADQLSIILKFLDPHNEGYISYHHFLQLFEQKENLSAHKWLNSNHKVVEPRKDLQLSSETIKSTISDNIGNKWSNVVHEFNLVDLNRTGYISVEDLKKIFKKLFGYLKDDDIDRLVAPLTLKDKKGRINYLELFNLLNVDTS------------------PSDLEGLSTQIAESN-DAQLKERHENHIEKMESIEKL-GEARLNLMSPEELIVRIKDRIVQNRLNYHELYKKYECNDK----KGGINKSQLRKMLTQFGYTLTNEQFSKISEILNFPTSSKSRIDFNGFL---AIFADNRESSNDLMTDVCFLPKSNHHVNDIRGDDNRNMNCQEVEDLLR--QKIVNNFPDLMIAFHKFDRSNKGYLVKSDFQNFLLGNLIMIENDQFDLLCEKLGVGEKSKLTYNDFLNRF 2666
++R +K + F PLT ++F+KL A + G++ Y EF++ +G+ + + +E+ N TK ++ E EL+Q V R K+ D + + K F +D + ++++ + K+L + ++ +++ + I +L FL+ ++ +S + K + PI P +++ + + ++ + AF +++ G + +EF ++L F ++ +Q L+ L H YI++ +FLQ F +A +W K P ++S I + + + + + + EF D +T IS E+ + + + L D+ DRL + + + KGR+ Y + + + + + P EG+ + ++ N + + + ++H S + G L P E R++ RI + + +L K EC +K +G I+ S+ ++ +F ++ E+ ++ I+ + + + + F+ + +ESS LM + ++ H + D + + + LLR KIV+ + + F +D + G L +DF+ L I + ++F + E S ++YNDFL F
Sbjct: 12 LRRRDIKNALYGFDIPLTPREFEKLWASYDTEGKGHITYQEFLQKLGV--NYSPAVHRPCAEDYFNFMGHFTKPQQLQEEM---------KELQQSTEKAVAARDKLVDCYQDISKAFTKIDKSKTNYISICKMQKVLEECGCSLTEGELTNLLNSWGVSRHDNSINYLDFLRAVENSKS-----TGAQPKEKEESMPINFATLNPQ--EVVRKIQEVVESSQLALSTAFSALDKEDTGFVKSTEFGQVLKDFCHKLTDNQYHYFLRKLRIHLTPYINWKYFLQNFSCFLEETAEEWAEKMPKGPPPTSPKAMASRDILARLHKAVTSHYHAITQEFENFDTMKTNTISREEFRAVCNRNVQILTDEQFDRLWNEMPV-NAKGRLKYPDFLSRFSSERAATPTATGDSAAAQRGSSVPDVSEGIRSALSLPNQELRPGSKPQSHPCTAASTTAIPGTPPLQNCDPIE--SRLRKRI---QGCWRQLLK--ECKEKDVARQGDISASEFLALVEKFNLDISKEECQQL--IIKYDLKNNGKFAYCDFIQSCVLLLKAKESS--LMQRMKI--QNAHKMKDSGAETSSFYSA-----LLRIQPKIVHCWRPMRRTFKSYDEAGTGLLSVADFRTVLRQYSINLSEEEFFHILEYYDKTLSSNISYNDFLRAF 644 HSP 2 Score: 92.4337 bits (228), Expect = 1.418e-17 Identity = 91/420 (21.67%), Postives = 171/420 (40.71%), Query Frame = 3
Query: 2826 FIALVTSGTGFIRFRNLQHLLSRLQISMSKSEMKKLWKRYDTNNRNKLDYHDFLYNLGLLEKFGCGDLMGPSAAIIQN--SHNALVDHLVNQETKHQQSVDNQAVLGTHLHVEHLFEMVKRHLRVAYSDSALAFKAVANKKTLSGFFTINDLNKLLSQLHMFVTNDKFVQLAARMGFEGPDDSLSYEKFCCIFQDSLNIDGQEK-TVGSIRINQDLENPSKIGIEEAGNLNSTQIASIIGKRIVDQYSEIEKTFLVMDPENSGYVSMNEFRNLLVGFCLSLTDKQFNKYRNSLSTYIVKSANTDDKIDYRYFMKGFN----QKLNNLDIQXXXXXXXXXXXXXTYQQIQEHINESIKVRPKLFLNSFKSKDYASLNFISKSDFREIIERFLFTVPDSHFEKFWSNLKKNEFDNVNYGEFV 4064
F+ G G +R R++++ L I ++ E +KLW YDT + + Y +FL LG+ + P A N H L + + QQS + + L Y D + AF + KT + +I + K+L + +T + L G D+S++Y F ++S + Q K S+ IN NP ++ + + S+Q+A + F +D E++G+V EF +L FC LTD Q++ + L ++ I+++YF++ F+ + + P + M + I +++++ F++ D N IS+ +FR + R + + D F++ W+ + N + Y +F+
Sbjct: 1 FLETDNEGNGILRRRDIKNALYGFDIPLTPREFEKLWASYDTEGKGHITYQEFLQKLGVNYSPA---VHRPCAEDYFNFMGHFTKPQQLQEEMKELQQSTEKAVA-------------ARDKLVDCYQDISKAFTKIDKSKT--NYISICKMQKVLEECGCSLTEGELTNLLNSWGVSRHDNSINYLDFLRAVENSKSTGAQPKEKEESMPINFATLNPQEVVRKIQEVVESSQLA-------------LSTAFSALDKEDTGFVKSTEFGQVLKDFCHKLTDNQYHYFLRKLRIHLTPY------INWKYFLQNFSCFLEETAEEWAEKMPKGPPPTSPKAMASRDILARLHKAVTSHYHAITQEFENFDTMKTNTISREEFRAVCNRNVQILTDEQFDRLWNEMPVNAKGRLKYPDFL 383 HSP 3 Score: 87.8113 bits (216), Expect = 4.338e-16 Identity = 125/661 (18.91%), Postives = 266/661 (40.24%), Query Frame = 3
Query: 1431 FLQINQTHKGKIPKSEFLKILNRFQFYISADQLSIILKFLDPHNEGYISYHHFLQLFEQKENLSAHKWLNSNH-----------KVVEPRKDLQLSSETIKSTISDNIGNKWSNVVHEFNLVDLNRTGYISVEDLKKIFKKLFGYLKDDDIDRLVAPLTLKDKKGRINYLELFNLLNVDTSPSDLEGLSTQIAESNDAQLKERHENHIEKMESIEKLGEARLNLMSPEELIVRIKDRIVQNRLNYHELYKKYECNDKKGGINKSQLRKMLTQFGYTLTNEQFSKISEILNFPTSSKSRIDFNGFLAIFADNRESSNDLMTDVCFLPKSNHHVNDIRGDDNRNMNCQEVEDLLRQKIVNNFPDLMIAFHKFDRSNKGYLVKSDFQNFLLGNLIMIENDQFDLLCEKLGVGEKSKLTYNDFLNRFQIR--------------------DDMVDGHKWLDS-------------KHRFNDPSTNAVPDMS----AEDVYMLLPEKIYQYFHEISPTFIALVTSGTGFIRFRNLQHLLSRLQISMSKSEMKKLWKRYDTNNRNKLDYHDFLYNLGLLEKFGCGDLMGPSAAIIQNSHNALVDHLVNQETKHQQSVDNQAVLGTHLHVEHLFEMVKRHLRVAYSDSALAFKAVANKKTLSGFFTIN 3269
FL+ + G + + + L F ++ + + D +G+I+Y FLQ + + H+ ++ ++ E K+LQ S+E + D + + + ++ F +D ++T YIS+ ++K+ ++ L + ++ L+ + INYL+ + + ++S AQ KE+ E+ + A LN P+E++ +I++ + ++L + + D G + ++ ++L F + LT+ Q+ L + I++ FL F+ E + + + +PK + + M +++ L + + +++ + F FD + + +F+ N+ ++ ++QFD L ++ V K +L Y DFL+RF D+ +G + S H ST A+P + + L ++I + ++ + G I L+ + + +SK E ++L +YD N K Y DF+ + LL K LM IQN+H +++ + S A+L + H + ++R + +Y ++ +VA+ +T+ ++IN
Sbjct: 1 FLETDNEGNGILRRRDIKNALYGFDIPLTPREFEKLWASYDTEGKGHITYQEFLQKLGVNYSPAVHRPCAEDYFNFMGHFTKPQQLQEEMKELQQSTEKAVAA-RDKLVDCYQDISKAFTKIDKSKTNYISICKMQKVLEECGCSLTEGELTNLLNSWGVSRHDNSINYLDFLR--------------AVENSKSTGAQPKEKEESM--------PINFATLN---PQEVVRKIQEVVESSQLALSTAFSALDKEDT-GFVKSTEFGQVLKDFCHKLTDNQYHYFLRKLRIHLTP--YINWKYFLQNFSCFLEETAEEWAEK--MPKGPPPTSP------KAMASRDILARLHKAVTSHYHAITQEFENFDTMKTNTISREEFRAVCNRNVQILTDEQFDRLWNEMPVNAKGRLKYPDFLSRFSSERAATPTATGDSAAAQRGSSVPDVSEGIRSALSLPNQELRPGSKPQSHPCTAASTTAIPGTPPLQNCDPIESRLRKRIQGCWRQLLKECKEKDVARQGDISASEFLALVEKFNLDISKEECQQLIIKYDLKNNGKFAYCDFIQSCVLLLKAKESSLM--QRMKIQNAHKM-------KDSGAETSSFYSALLRIQPKIVHCWRPMRRTFK-SYDEAGTGLLSVADFRTVLRQYSIN 614 HSP 4 Score: 70.4774 bits (171), Expect = 9.785e-11 Identity = 49/212 (23.11%), Postives = 100/212 (47.17%), Query Frame = 3
Query: 4161 TDEMDTDESIRKNLERLLNRNWQSIFNKCKAIDQAINECQTAEGSIPIDDFVYILSEYDDSLSAENLAAINL-YKTCKNGRFIKYGDLIRSIVL---SSRGSLISNENVTEVQKKFLNVGIKTTNSDNSKDYFPQLFKLRRQILENWKDLRKNFLQSDKKQNGAVSGNIFMTIMKTVCGQLSADLFYHLMKIYDQDGTGNFHYKRFIEDFLK 4784
T + + I L + + W+ + +CK D A +G I +F+ ++ +++ +S E + + Y NG+F Y D I+S VL + SL+ + K +K + ++ S ++ L +++ +I+ W+ +R+ F D+ G +S F T+++ LS + F+H+++ YD+ + N Y F+ FL+
Sbjct: 449 TPPLQNCDPIESRLRKRIQGCWRQLLKECKEKDVA------RQGDISASEFLALVEKFNLDISKEECQQLIIKYDLKNNGKFA-YCDFIQSCVLLLKAKESSLMQRMKIQNAHK------MKDSGAETS-SFYSALLRIQPKIVHCWRPMRRTFKSYDEAGTGLLSVADFRTVLRQYSINLSEEEFFHILEYYDKTLSSNISYNDFLRAFLQ 646 HSP 5 Score: 65.4698 bits (158), Expect = 3.067e-9 Identity = 112/580 (19.31%), Postives = 220/580 (37.93%), Query Frame = 3
Query: 711 IMKAFKLFDFNSDYCIQRHRLKKVMENFCCPLTEQQFDKLVARFSQSQNGN-VNYMEFMKGMGIPIDNQGTI--QTKNSEETINIDTRNTKGSRIFEAALIENGLLGSNELEQLLVIFRKKIEDQFSVLRKTFLVMDPNAEGFVTLEILYKILNQFAFPMSLNVFNQIMDKCAFKATGKIIWLSFLQKFQDPQSKHLNGSTIAIKSDHKCNPIQGVNSKPNIDDLLELLSNHISNNYSSFRDAFLQINQTHKGKIPKSEFLKILNRFQFYISADQLSIILKFLDPHNEGYISYHHFLQLFEQKE--------NLSAHKWLNSNHKVVE--------PRKDLQLSS------------------------ETIKSTISDNIGNKWSNVVHEFNLVDLNRTGYISVEDLKKIFKKLFGYLKDDDIDRLVAPLTLKDKKGRINYLELFNLLNVDTSPSDLEGLSTQIAESNDAQLKERHENHIEKMESIEKLGEARLNLMSPEELIVRIKDRIVQNRLNYHELYKKYECNDKKGGINKSQLRKMLTQFGYTLTNEQFSKISEILNFPTSSKSRIDFNGFLAIF 2321
I KAF D + I +++KV+E C LTE + L+ + S++ N +NY++F++ ++N + Q K EE++ I+ + ++++ ++ +E L F +D GFV ++L F ++ N ++ + K T I W FLQ F S L + P D+L L ++++Y + F + I + EF + NR ++ +Q + + + +G + Y FL F + + +A + +S V E P ++L+ S + I+S + I W ++ E D+ R G IS + + +K + ++ +L+ LK+ G+ Y + + + ++ ++ L +R K+++ K+ ++ S ++RI+ +IV +K Y+ G ++ + R +L Q+ L+ E+F I E + SS I +N FL F
Sbjct: 114 ISKAFTKIDKSKTNYISICKMQKVLEECGCSLTEGELTNLLNSWGVSRHDNSINYLDFLRA----VENSKSTGAQPKEKEESMPIN-------------------FATLNPQEVVRKIQEVVESSQLALSTAFSALDKEDTGFVKSTEFGQVLKDFCHKLTDNQYHYFLRKLRIHLTPYINWKYFLQNF----SCFLEETAEEWAEKMPKGPPPTSPKAMASRDILARLHKAVTSHYHAITQEFENFDTMKTNTISREEFRAVCNRNVQILTDEQFDRLWNEMPVNAKGRLKYPDFLSRFSSERAATPTATGDSAAAQRGSSVPDVSEGIRSALSLPNQELRPGSKPQSHPCTAASTTAIPGTPPLQNCDPIESRLRKRIQGCWRQLLKECKEKDVARQGDISASEFLALVEKFNLDISKEECQQLIIKYDLKN-NGKFAYCDFIQSCVL-------------LLKAKESSLMQRM-----KIQNAHKMKDSGAETSSFYSALLRIQPKIVHCWRPMRRTFKSYD-EAGTGLLSVADFRTVLRQYSINLSEEEFFHILEYYDKTLSSN--ISYNDFLRAF 644
BLAST of EF-hand calcium-binding domain-containing protein 6 vs. TrEMBL
Match: A0A267H1X4 (Uncharacterized protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig003760g2 PE=4 SV=1) HSP 1 Score: 682.559 bits (1760), Expect = 0.000e+0 Identity = 438/1465 (29.90%), Postives = 750/1465 (51.19%), Query Frame = 3
Query: 117 QSNSLNQPEKNSTIKDANYKMDPKLQISEINPIVKSQLLKNHKEILKALQTFDIECSGLVTKGELRRVLDSYCLPMTTDQYEQFLSHNSINYSKGSINYKELIDKIYDKTSAVGHFKKNIKLPQLQRSATTICLQNKHFTLPTTRRSSGKISRSQLFXXXXXXXXXXXXXXXXWNYNAYTIDDIIPILQMQISRNPNGIMKAFKLFDFNSDYCIQRHRLKKVMENFCCPLTEQQFDKLVARFSQSQNGNVNYMEFMKGMGIPIDNQGTIQTKNSEETINIDTRNTKGSRIFEAALIENGLLGSNELEQLLVIFRKKIEDQFSVLRKTFLVMDPNAEGFVTLEILYKILNQFAFPMSLNVFNQIMDKCAFKATGKIIWLSFLQKFQDPQSKHLNGSTIAIKSDHKCNPIQGVNSKPNIDDLLELLSNHISNNYSSFRDAFLQINQTHKGKIPKSEFLKILNRFQFYISADQLSIILKFLDPHNEGYISYHHFLQLFEQKENLSAHKWLNSNHKVVEPRKDLQLSSETIKSTISDNIGNKWSNVVHEFNLVDLNRTGYISVEDLKKIFKKLFGYLKDDDIDRLVAPLTLKDKKGRINYLELFNLLNVDTSPSDLEGLSTQIAESNDAQLKERHENHIEKMESIEKLGEARLNLMSPEELIVRIKDRIVQNRLNYHELYKKYECNDKKGGINKSQLRKMLTQFGYTLTNEQFSKISEILNFPTSSKSRIDFNGFLAIFADNRESSNDLMTDVCFLPKSNHHVNDIRGDDNRNMNCQEVEDLLRQKIVNNFPDLMIAFHKFDRSNKGYLVKSDFQNFLLGNLIMIEND-QFDLLCEKLGVGEKSKLTYNDFLNRFQIRDDMVDGHKWLDSKHRFNDPSTNAVPDMSAEDVYMLLPEKIYQYFHEISPTFIALVTSGTGFIRFRNLQHLLSRLQISMSKSEMKKLWKRYDTNNRNKLDYHDFLYNLGLLEKFGCGDLMGPSAAIIQNSHNALVDHLVNQETKHQQSVDNQAVLGTHLHVEHLFEMVKRHLRVAYSDSALAFKAVANKKTLSGFFTINDLNKLLSQLHMFVTNDKFVQLAARMGFEGPDDSLSYEKFCCIFQDSLNIDGQE---KTVGSIRINQDLENPSKIGIEEAGNLNSTQIASIIGKRIVDQYSEIEKTFLVMDPENSGYVSMNEFRNLLVGFCLSLTDKQFNKYRNSLSTYIVKSANTDDKIDYRYFMKGFNQKLNNLDIQX----------XXXXXXXXXXXXTYQQIQEHINESIKVRPKLFLNSFKSKDYASLNFISKSDFREIIERFLFTVPDSHFEKFWSNLKKNEFDNVNYGEFVKMXXXXXXXXXXXXXXXX----------XXXXXXXXXXNNIETDEMDTDESIRKNLERLLNRNWQSIFNKCKAIDQAINECQTAEGSIPIDDFVYIL-SEYDDSLSAENLAAINLYKTCKNGRFIKYGDLIRSIV 4436
Q+N Q S + A+ P Q S ++ + + +N E+L A + +DIE +G VTKGELRR+++ + T + + L + ++N + GS+ Y + K Y +G + ++ A L K P N NA I+ +L+ I N + +++ F+LFD+N D IQ H L+K++E +C P++ QF+KL S+ G ++YM+F+ +G+ + N G+ N+ R E ++ G G N ++QL V +R+++++Q +L+K LV+D G + ++ IL++F +S F+++M++ + KA+ I W FLQKF DPQS NG T+ I+ HK N ++G + + ++ DL++LL NHI + YSS R+AFLQI+ KGKI ++F +++ F+F +S Q+ I+ LDP + G+I+Y FL +FE+KE+ HKWL S HK + K L+ +T++ + I +W + D TGYI+ +LKK+ K+ L D+ D L+ L+ K G++NYLE ++ +D P D+EG+S QIA N+A+L+ R H + M IE R N+MS EE+IVR++DR Q R + + + + + + +KG ++K++ R L G L++E FS+++ L F + +D+ F+ F + R+S + +LP NH N +RGD+ M+ VE+ LR KI NF L AFHK D +GYL K DF+ LL L+ + ND +F+ LC KLG+ + KL+Y DFL +FQ +DD+ GHKWL S HR+N+ + +A +V+ L+ EK++Q + +I+ F + G G+I R + LL RL ++M + E KKLW YDT NRN + + FL +G+ F GD G SA IIQNS + HL QE + + V NQAVL L + LF ++++ +R + + AF+A+ K+ G+ + D + Q+H+FV ++ F +L R+G P ++YE FC F + + + KT G + ++ + + ++ + +V +EK F MD + + ++FR ++ GFC LTD QF + L + D++DY F+ F+Q + + + A + +++ Q+++ ++E + RP L +F DYA L ++K+DFR+I+ R + + + F++ + + NE+ NV+Y EF+ + + + ++ + + +++ + + + L R ++++W+++F++ K +DQA + I +DDF +L S L+ +A I + + +Y D +R+ V
Sbjct: 9 QTNFAAQGRNGSIMPVASTSRTP--QASAVDIAISKHVKENFDELLSAFKVYDIENNGSVTKGELRRIIEINITAINTADFGKVLKNATVNRN-GSVQYMTFL-KFYKD---LGQGASKEAEGEKRKQADATGLNGKKPIDP--------------------------------NLNAGEIE---KLLKNAIESNLSSVLRGFRLFDYNRDRKIQFHELRKILEQYCFPMSNVQFEKLWNSIDYSKMGLIDYMDFLGRLGVAVPNAGSK---------NVGQRT-------ERKKLQLGSSGMN-MDQLEVEYRRRLKEQHGLLKKACLVVDSKHSGLMPIKNFKAILDEFVIAVSDQEFSKLMERFSVKASHDIQWYGFLQKFVDPQSLS-NGQTLPIRPSHKANVVRGSHVEASVQDLIQLLYNHILSAYSSLRNAFLQIDINRKGKITIADFRRLVQGFKFELSEVQIKEIMVQLDPEHTGFITYQKFLDIFEEKESADGHKWLVSTHKYNKDTKPPILAWKTVEDLLHQKIVLRWKSFTAALKHYDSTGTGYINPTNLKKVLDKIAIPLSDEHYDSLLKRLSHKTADGKVNYLEFLAVMQIDVYPGDVEGVSAQIASGNEARLQMRQSQHEKAMADIETHASLRTNMMSLEEIIVRLRDRSSQTRQHIRDAFLQMD-DARKGFVSKAKFRLFLESHGIFLSDENFSQLTRELEF---GREGLDYMRFVKHF-EGRDSE----LNPSYLPMQNHKYNPVRGDEF-GMSAPAVENKLRSKIRENFTTLREAFHKMDSDGRGYLTKQDFRR-LLDQLMCLTNDAEFNKLCNKLGIDDDCKLSYRDFLGKFQEKDDLATGHKWLVSNHRYNE--LGDIKPKTAAEVHQLMAEKVHQNYKDIARAFCHIDYLGRGYITKREFKELLVRLNVTMDQEEFKKLWAYYDTANRNAITHDAFLKRVGVASAFSSGDGQGTSAKIIQNSAEYIAVHLEGQENRQENLVFNQAVLTVGLDADALFIILQKRIRDFFPTTYAAFRALDGKE--KGYLSQEDFRIAVHQMHLFVRDESFAELMRRIGLSQPTAQMTYEAFCLAFDEKFSGPRESQRPKTAGPM-LDGARGGQQQQQQQPQQRQDAKAFLKRLRASVVKDGEILEKVFRSMDTKQESRIGASDFRRVVDGFCFKLTDAQFKEVVKGL-------LSQGDQVDYYRFLANFSQTQEAAERREWMEAANGVGGGAGGSAEDGESLSQQEVEARVSEFVSARPHLLAQAFAEADYAQLGVLAKTDFRDILFRLVMRMSNEQFDQLMTRVPTNEYGNVDYKEFLSRFASAEQPQQQQQQPTRPDSPISLTPPASADQAQQQQQQPQQPTLSLETEAEAQLRRAMSQSWKTLFSRFKLLDQATPD---KPKQIHVDDFCAVLRSSGAVDLAPSQVAPILRRFGLRGDGYFRYQDCMRAFV 1387
BLAST of EF-hand calcium-binding domain-containing protein 6 vs. TrEMBL
Match: A0A267FVL1 (Uncharacterized protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig003760g3 PE=4 SV=1) HSP 1 Score: 679.863 bits (1753), Expect = 0.000e+0 Identity = 437/1470 (29.73%), Postives = 745/1470 (50.68%), Query Frame = 3
Query: 117 QSNSLNQPEKNSTIKDANYKMDPKLQISEINPIVKSQLLKNHKEILKALQTFDIECSGLVTKGELRRVLDSYCLPMTTDQYEQFLSHNSINYSKGSINYKELIDKIYDKTSAVGHFKKNIKLPQLQRSATTICLQNKHFTLPTTRRSSGKISRSQLFXXXXXXXXXXXXXXXXWNYNAYTIDDIIPILQMQISRNPNGIMKAFKLFDFNSDYCIQRHRLKKVMENFCCPLTEQQFDKLVARFSQSQNGNVNYMEFMKGMGIPIDNQGTIQTKNSEETINIDTRNTKGSRIFEAALIENGLLGSNELEQLLVIFRKKIEDQFSVLRKTFLVMDPNAEGFVTLEILYKILNQFAFPMSLNVFNQIMDKCAFKATGKIIWLSFLQKFQDPQSKHLNGSTIAIKSDHKCNPIQGVNSKPNIDDLLELLSNHISNNYSSFRDAFLQINQTHKGKIPKSEFLKILNRFQFYISADQLSIILKFLDPHNEGYISYHHFLQLFEQKENLSAHKWLNSNHKVVEPRKDLQLSSETIKSTISDNIGNKWSNVVHEFNLVDLNRTGYISVEDLKKIFKKLFGYLKDDDIDRLVAPLTLKDKKGRINYLELFNLLNVDTSPSDLEGLSTQIAESNDAQLKERHENHIEKMESIEKLGEARLNLMSPEELIVRIKDRIVQNRLNYHELYKKYECNDKKGGINKSQLRKMLTQFGYTLTNEQFSKISEILNFPTSSKSRIDFNGFLAIFADNRESSNDLMTDVCFLPKSNHHVNDIRGDDNRNMNCQEVEDLLRQKIVNNFPDLMIAFHKFDRSNKGYLVKSDFQNFLLGNLIMIEND-QFDLLCEKLGVGEKSKLTYNDFLNRFQIRDDMVDGHKWLDSKHRFNDPSTNAVPDMSAEDVYMLLPEKIYQYFHEISPTFIALVTSGTGFIRFRNLQHLLSRLQISMSKSEMKKLWKRYDTNNRNKLDYHDFLYNLGLLEKFGCGDLMGPSAAIIQNSHNALVDHLVNQETKHQQSVDNQAVLGTHLHVEHLFEMVKRHLRVAYSDSALAFKAVANKKTLSGFFTINDLNKLLSQLHMFVTNDKFVQLAARMGFEGPDDSLSYEKFCCIFQDSLNIDGQE---KTVGSIRINQDLENPSKIGIEEAGNLNSTQIASIIGKRIVDQYSEIEKTFLVMDPENSGYVSMNEFRNLLVGFCLSLTDKQFNKYRNSLSTYIVKSANTDDKIDYRYFMKGFNQKLNNLDIQXXXXXXXXXX----------XXXTYQQIQEHINESIKVRPKLFLNSFKSKDYASLNFISKSDFREIIERFLFTVPDSHFEKFWSNLKKNEFDNVNYGEFVKMXXXXXXXXXXXXXXXXXXXXXXXXXXN---------------NIETDEMDTDESIRKNLERLLNRNWQSIFNKCKAIDQAINECQTAEGSIPIDDFVYIL-SEYDDSLSAENLAAINLYKTCKNGRFIKYGDLIRSIV 4436
Q+N Q S + A+ P Q S ++ + + +N E+L A + +DIE +G VTKGELRR+++ + T + + L + ++N + GS+ Y + K Y +G + ++ A L K P N NA I+ +L+ I N + +++ F+LFD+N D IQ H L+K++E +C P++ QF+KL S+ G ++YM+F+ +G+ + N G+ N+ R E ++ G G N ++QL V +R+++++Q +L+K LV+D G + ++ IL++F +S F+++M++ + KA+ I W FLQKF DPQS NG T+ I+ HK N ++G + + ++ DL++LL NHI + YSS R+AFLQI+ KGKI ++F +++ F+F +S Q+ I+ LDP + G+I+Y FL +FE+KE+ HKWL S HK + K L+ +T++ + I +W + D TGYI+ +LKK+ K+ L D+ D L+ L+ K G++NYLE ++ +D P D+EG+S QIA N+A+L+ R H + M IE R N+MS EE+IVR++DR Q R + + + + + + +KG ++K++ R L G L++E FS+++ L F + +D+ F+ F + R+S + +LP NH N +RGD+ M+ VE+ LR KI NF L AFHK D +GYL K DF+ LL L+ + ND +F+ LC KLG+ + KL+Y DFL +FQ +DD+ GHKWL S HR+N+ + +A +V+ L+ EK++Q + +I+ F + G G+I R + LL RL ++M + E KKLW YDT NRN + + FL +G+ F GD G SA IIQNS + HL QE + + V NQAVL L + LF ++++ +R + + AF+A+ K+ G+ + D + Q+H+FV ++ F +L R+G P ++YE FC F + + + KT G + ++ + + ++ + +V +EK F MD + + ++FR ++ GFC LTD QF + L + D++DY F+ F+Q + + +++ Q+++ ++E + RP L +F DYA L ++K+DFR+I+ R + + + F++ + + NE+ NV+Y EF+ + + + ++ + + + + + + L R ++++W+++F++ K +DQA + I +DDF +L S L+ +A I + + +Y D +R+ V
Sbjct: 9 QTNFAAQGRNGSIMPVASTSRTP--QASAVDIAISKHVKENFDELLSAFKVYDIENNGSVTKGELRRIIEINITAINTADFGKVLKNATVNRN-GSVQYMTFL-KFYKD---LGQGASKEAEGEKRKQADATGLNGKKPIDP--------------------------------NLNAGEIE---KLLKNAIESNLSSVLRGFRLFDYNRDRKIQFHELRKILEQYCFPMSNVQFEKLWNSIDYSKMGLIDYMDFLGRLGVAVPNAGSK---------NVGQRT-------ERKKLQLGSSGMN-MDQLEVEYRRRLKEQHGLLKKACLVVDSKHSGLMPIKNFKAILDEFVIAVSDQEFSKLMERFSVKASHDIQWYGFLQKFVDPQSLS-NGQTLPIRPSHKANVVRGSHVEASVQDLIQLLYNHILSAYSSLRNAFLQIDINRKGKITIADFRRLVQGFKFELSEVQIKEIMVQLDPEHTGFITYQKFLDIFEEKESADGHKWLVSTHKYNKDTKPPILAWKTVEDLLHQKIVLRWKSFTAALKHYDSTGTGYINPTNLKKVLDKIAIPLSDEHYDSLLKRLSHKTADGKVNYLEFLAVMQIDVYPGDVEGVSAQIASGNEARLQMRQSQHEKAMADIETHASLRTNMMSLEEIIVRLRDRSSQTRQHIRDAFLQMD-DARKGFVSKAKFRLFLESHGIFLSDENFSQLTRELEF---GREGLDYMRFVKHF-EGRDSE----LNPSYLPMQNHKYNPVRGDEF-GMSAPAVENKLRSKIRENFTTLREAFHKMDSDGRGYLTKQDFRR-LLDQLMCLTNDAEFNKLCNKLGIDDDCKLSYRDFLGKFQEKDDLATGHKWLVSNHRYNE--LGDIKPKTAAEVHQLMAEKVHQNYKDIARAFCHIDYLGRGYITKREFKELLVRLNVTMDQEEFKKLWAYYDTANRNAITHDAFLKRVGVASAFSSGDGQGTSAKIIQNSAEYIAVHLEGQENRQENLVFNQAVLTVGLDADALFIILQKRIRDFFPTTYAAFRALDGKE--KGYLSQEDFRIAVHQMHLFVRDESFAELMRRIGLSQPIAQMTYEAFCLAFDEKFSGPRESQRPKTAGPM-LDGARGGQQQQQQQPQQRQDAKAFLKRLRASVVKDGEILEKVFRSMDTKQESRIGASDFRRVVDGFCFKLTDAQFKEVVKGL-------LSQGDQVDYYRFLANFSQTQEAAERREWMEAANGGGGGAGGSAEDGESLSQQEVEARVSEFVSARPHLLAQAFAEADYAQLGVLAKTDFRDILFRLVMRMSNEQFDQLMTRVPTNEYGNVDYKEFLSRFASAEQPQQQQQQPTAGGDARPDSPISLTPPASADQAQQQQQQPQQPTLSLETEAEAQLRRAMSQSWKTLFSRFKLLDQATPD---KPKQIHVDDFCAVLRSSGAVDLAPSQVAPILRRFGLRGDGYFRYQDCMRAFV 1392
BLAST of EF-hand calcium-binding domain-containing protein 6 vs. TrEMBL
Match: A0A267DTE8 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig003760g1 PE=4 SV=1) HSP 1 Score: 679.093 bits (1751), Expect = 0.000e+0 Identity = 437/1470 (29.73%), Postives = 745/1470 (50.68%), Query Frame = 3
Query: 117 QSNSLNQPEKNSTIKDANYKMDPKLQISEINPIVKSQLLKNHKEILKALQTFDIECSGLVTKGELRRVLDSYCLPMTTDQYEQFLSHNSINYSKGSINYKELIDKIYDKTSAVGHFKKNIKLPQLQRSATTICLQNKHFTLPTTRRSSGKISRSQLFXXXXXXXXXXXXXXXXWNYNAYTIDDIIPILQMQISRNPNGIMKAFKLFDFNSDYCIQRHRLKKVMENFCCPLTEQQFDKLVARFSQSQNGNVNYMEFMKGMGIPIDNQGTIQTKNSEETINIDTRNTKGSRIFEAALIENGLLGSNELEQLLVIFRKKIEDQFSVLRKTFLVMDPNAEGFVTLEILYKILNQFAFPMSLNVFNQIMDKCAFKATGKIIWLSFLQKFQDPQSKHLNGSTIAIKSDHKCNPIQGVNSKPNIDDLLELLSNHISNNYSSFRDAFLQINQTHKGKIPKSEFLKILNRFQFYISADQLSIILKFLDPHNEGYISYHHFLQLFEQKENLSAHKWLNSNHKVVEPRKDLQLSSETIKSTISDNIGNKWSNVVHEFNLVDLNRTGYISVEDLKKIFKKLFGYLKDDDIDRLVAPLTLKDKKGRINYLELFNLLNVDTSPSDLEGLSTQIAESNDAQLKERHENHIEKMESIEKLGEARLNLMSPEELIVRIKDRIVQNRLNYHELYKKYECNDKKGGINKSQLRKMLTQFGYTLTNEQFSKISEILNFPTSSKSRIDFNGFLAIFADNRESSNDLMTDVCFLPKSNHHVNDIRGDDNRNMNCQEVEDLLRQKIVNNFPDLMIAFHKFDRSNKGYLVKSDFQNFLLGNLIMIEND-QFDLLCEKLGVGEKSKLTYNDFLNRFQIRDDMVDGHKWLDSKHRFNDPSTNAVPDMSAEDVYMLLPEKIYQYFHEISPTFIALVTSGTGFIRFRNLQHLLSRLQISMSKSEMKKLWKRYDTNNRNKLDYHDFLYNLGLLEKFGCGDLMGPSAAIIQNSHNALVDHLVNQETKHQQSVDNQAVLGTHLHVEHLFEMVKRHLRVAYSDSALAFKAVANKKTLSGFFTINDLNKLLSQLHMFVTNDKFVQLAARMGFEGPDDSLSYEKFCCIFQDSLNIDGQE---KTVGSIRINQDLENPSKIGIEEAGNLNSTQIASIIGKRIVDQYSEIEKTFLVMDPENSGYVSMNEFRNLLVGFCLSLTDKQFNKYRNSLSTYIVKSANTDDKIDYRYFMKGFNQKLNNLDIQXXXXXXXXXX----------XXXTYQQIQEHINESIKVRPKLFLNSFKSKDYASLNFISKSDFREIIERFLFTVPDSHFEKFWSNLKKNEFDNVNYGEFVKMXXXXXXXXXXXXXXXXXXXXXXXXXXN---------------NIETDEMDTDESIRKNLERLLNRNWQSIFNKCKAIDQAINECQTAEGSIPIDDFVYIL-SEYDDSLSAENLAAINLYKTCKNGRFIKYGDLIRSIV 4436
Q+N Q S + A+ P Q S ++ + + +N E+L A + +DIE +G VTKGELRR+++ + T + + L + ++N + GS+ Y + K Y +G + ++ A L K P N NA I+ + L+ I N + +++ F+LFD+N D IQ H L+K++E +C P++ QF+KL S+ G ++YM+F+ +G+ + N G+ N+ R E ++ G G N ++QL V +R+++++Q +L+K LV+D G + ++ IL++F +S F+++M++ + KA+ I W FLQKF DPQS NG T+ I+ HK N ++G + + ++ DL++LL NHI + YSS R+AFLQI+ KGKI ++F +++ F+F +S Q+ I+ LDP + G+I+Y FL +FE+KE+ HKWL S HK + K L+ +T++ + I +W + D TGYI+ +LKK+ K+ L D+ D L+ L+ K G++NYLE ++ +D P D+EG+S QIA N+A+L+ R H + M IE R N+MS EE+IVR++DR Q R + + + + + + +KG ++K++ R L G L++E FS+++ L F + +D+ F+ F + R+S + +LP NH N +RGD+ M+ VE+ LR KI NF L AFHK D +GYL K DF+ LL L+ + ND +F+ LC KLG+ + KL+Y DFL +FQ +DD+ GHKWL S HR+N+ + +A +V+ L+ EK++Q + +I+ F + G G+I R + LL RL ++M + E KKLW YDT NRN + + FL +G+ F GD G SA IIQNS + HL QE + + V NQAVL L + LF ++++ +R + + AF+A+ K+ G+ + D + Q+H+FV ++ F +L R+G P ++YE FC F + + + KT G + ++ + + ++ + +V +EK F MD + + ++FR ++ GFC LTD QF + L + D++DY F+ F+Q + + +++ Q+++ ++E + RP L +F DYA L ++K+DFR+I+ R + + + F++ + + NE+ NV+Y EF+ + + + ++ + + + + + + L R ++++W+++F++ K +DQA + I +DDF +L S L+ +A I + + +Y D +R+ V
Sbjct: 9 QTNFAAQGRNGSIMPVASTSRTP--QASAVDIAISKHVKENFDELLSAFKVYDIENNGSVTKGELRRIIEINITAINTADFGKVLKNATVNRN-GSVQYMTFL-KFYKD---LGQGASKEAEGEKRKQADATGLNGKKPIDP--------------------------------NLNAGEIEKL---LKNAIESNLSSVLRGFRLFDYNRDRKIQFHELRKILEQYCFPMSNVQFEKLWNSIDYSKMGLIDYMDFLGRLGVAVPNAGSK---------NVGQRT-------ERKKLQLGSSGMN-MDQLEVEYRRRLKEQHGLLKKACLVVDSKHSGLMPIKNFKAILDEFVIAVSDQEFSKLMERFSVKASHDIQWYGFLQKFVDPQSLS-NGQTLPIRPSHKANVVRGSHVEASVQDLIQLLYNHILSAYSSLRNAFLQIDINRKGKITIADFRRLVQGFKFELSEVQIKEIMVQLDPEHTGFITYQKFLDIFEEKESADGHKWLVSTHKYNKDTKPPILAWKTVEDLLHQKIVLRWKSFTAALKHYDSTGTGYINPTNLKKVLDKIAIPLSDEHYDSLLKRLSHKTADGKVNYLEFLAVMQIDVYPGDVEGVSAQIASGNEARLQMRQSQHEKAMADIETHASLRTNMMSLEEIIVRLRDRSSQTRQHIRDAFLQMD-DARKGFVSKAKFRLFLESHGIFLSDENFSQLTRELEF---GREGLDYMRFVKHF-EGRDSE----LNPSYLPMQNHKYNPVRGDEF-GMSAPAVENKLRSKIRENFTTLREAFHKMDSDGRGYLTKQDFRR-LLDQLMCLTNDAEFNKLCNKLGIDDDCKLSYRDFLGKFQEKDDLATGHKWLVSNHRYNE--LGDIKPKTAAEVHQLMAEKVHQNYKDIARAFCHIDYLGRGYITKREFKELLVRLNVTMDQEEFKKLWAYYDTANRNAITHDAFLKRVGVASAFSSGDGQGTSAKIIQNSAEYIAVHLEGQENRQENLVFNQAVLTVGLDADALFIILQKRIRDFFPTTYAAFRALDGKE--KGYLSQEDFRIAVHQMHLFVRDESFAELMRRIGLSQPIAQMTYEAFCLAFDEKFSGPRESQRPKTAGPM-LDGARGGQQQQQQQPQQRQDAKAFLKRLRASVVKDGEILEKVFRSMDTKQESRIGASDFRRVVDGFCFKLTDAQFKEVVKGL-------LSQGDQVDYYRFLANFSQTQEAAERREWMEAANGGGGGAGGSAEDGESLSQQEVEARVSEFVSARPHLLAQAFAEADYAQLGVLAKTDFRDILFRLVMRMSNEQFDQLMTRVPTNEYGNVDYKEFLSRFASAEQPQQQQQQPTAGGDARPDSPISLTPPASADQAQQQQQQPQQPTLSLETEAEAQLRRAMSQSWKTLFSRFKLLDQATPD---KPKQIHVDDFCAVLRSSGAVDLAPSQVAPILRRFGLRGDGYFRYQDCMRAFV 1392
BLAST of EF-hand calcium-binding domain-containing protein 6 vs. TrEMBL
Match: A0A267EGC4 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig025834g1 PE=4 SV=1) HSP 1 Score: 677.552 bits (1747), Expect = 0.000e+0 Identity = 428/1410 (30.35%), Postives = 729/1410 (51.70%), Query Frame = 3
Query: 117 QSNSLNQPEKNSTIKDANYKMDPKLQISEINPIVKSQLLKNHKEILKALQTFDIECSGLVTKGELRRVLDSYCLPMTTDQYEQFLSHNSINYSKGSINYKELIDKIYDKTSAVGHFKKNIKLPQLQRSATTICLQNKHFTLPTTRRSSGKISRSQLFXXXXXXXXXXXXXXXXWNYNAYTIDDIIPILQMQISRNPNGIMKAFKLFDFNSDYCIQRHRLKKVMENFCCPLTEQQFDKLVARFSQSQNGNVNYMEFMKGMGIPIDNQGTIQTKNSEETINIDTRNTKGSRIFEAALIENGLLGSNELEQLLVIFRKKIEDQFSVLRKTFLVMDPNAEGFVTLEILYKILNQFAFPMSLNVFNQIMDKCAFKATGKIIWLSFLQKFQDPQSKHLNGSTIAIKSDHKCNPIQGVNSKPNIDDLLELLSNHISNNYSSFRDAFLQINQTHKGKIPKSEFLKILNRFQFYISADQLSIILKFLDPHNEGYISYHHFLQLFEQKENLSAHKWLNSNHKVVEPRKDLQLSSETIKSTISDNIGNKWSNVVHEFNLVDLNRTGYISVEDLKKIFKKLFGYLKDDDIDRLVAPLTLKDKKGRINYLELFNLLNVDTSPSDLEGLSTQIAESNDAQLKERHENHIEKMESIEKLGEARLNLMSPEELIVRIKDRIVQNRLNYHELYKKYECNDKKGGINKSQLRKMLTQFGYTLTNEQFSKISEILNFPTSSKSRIDFNGFLAIFADNRESSNDLMTDVCFLPKSNHHVNDIRGDDNRNMNCQEVEDLLRQKIVNNFPDLMIAFHKFDRSNKGYLVKSDFQNFLLGNLIMIEND-QFDLLCEKLGVGEKSKLTYNDFLNRFQIRDDMVDGHKWLDSKHRFNDPSTNAVPDMSAEDVYMLLPEKIYQYFHEISPTFIALVTSGTGFIRFRNLQHLLSRLQISMSKSEMKKLWKRYDTNNRNKLDYHDFLYNLGLLEKFGCGDLMGPSAAIIQNSHNALVDHLVNQETKHQQSVDNQAVLGTHLHVEHLFEMVKRHLRVAYSDSALAFKAVANKKTLSGFFTINDLNKLLSQLHMFVTNDKFVQLAARMGFEGPDDSLSYEKFCCIFQDSLNIDGQE---KTVGSIRINQDLENPSKIGIEEAGNLNSTQIASIIGKRIVDQYSEIEKTFLVMDPENSGYVSMNEFRNLLVGFCLSLTDKQFNKYRNSLSTYIVKSANTDDKIDYRYFMKGFNQKLNNLDIQX----------XXXXXXXXXXXXTYQQIQEHINESIKVRPKLFLNSFKSKDYASLNFISKSDFREIIERFLFTVPDSHFEKFWSNLKKNEFDNVNYGEFVKMXXXXXXXXXXXXXXXXXXXXXXXXXXN-------------NIETDEMDTDESIRKNLERLLNRNWQSIFNKCKAIDQA 4265
Q+N Q S + ++ P Q S ++ + + + +N E+L A + +DIE +G VTKGE+RR+++ + T + + L + ++N + GS+ Y + K Y +G + ++ A L K P N NA I+ +L+ I N + +++ F+LFD+N D IQ H L+K++E +C P++ QF+KL S+ G ++YM+F+ +G+ + N G+ NI R E ++ G G N ++QL V +R+++++Q +L+K LV+D G + ++ IL++F +S F+++M++ + KA+ I W FLQKF DPQS NG T+ I+ HK N ++G + + ++ DL++LL NHI + YSS R+AFLQI+ KGKI ++F +++ F+F +S Q+ I+ LDP + G+I+Y FL +FE+KE+ HKWL S HK + K L+ +T++ + I +W + D TGYI+ +LKK+ K+ L D+ D L+ L+ K G++NYLE ++ +D P D+EG+S QIA N+A+L+ R H + M IE R N+MS EE+IVR++DR Q R + + + + + + +KG ++K++ R L G L++E FS+++ L F + +D+ F+ F + R+S + +LP NH N +RGD+ M+ Q VE+ LR KI NF L AFHK D +GYL K DF+ LL L+ + ND +F+ LC+KLG+ + KL+Y DFL +FQ +DD+ GHKWL S HR+N+ + +A +V+ L+ EK++Q + +I+ F + G G+I R + LL RL ++M++ E KKLW YDT NRN + + FL +G+ F GD G SA IIQNS + HL QE + + V NQAVL L + LF ++++ +R + + AF+A+ K+ G+ + D + Q+H+FV ++ F +L R+G P ++YE FC F + + + KT G + ++ + + ++ + +V +EK F MD + G + ++FR ++ GFC LTD QF + L + D++DY F+ F+Q + + + A +++ Q+++ ++E + RP L +F DYA L ++K+DFR+I+ R + + + F++ + + NE+ NV+Y EF+ + + + ++ + + + + + D L R ++++W+S+F++ K +DQA
Sbjct: 9 QANFAAQGSNGSIMPVSSTSRTP--QASAVDIAISNHVKENFDELLSAFKVYDIENNGSVTKGEMRRIIEINITAINTADFGKVLKNATVNRN-GSVQYMTFL-KFYKD---LGQGASKEAEGEKRKQADATGLNGKKPIDP--------------------------------NLNAGEIE---KLLKNAIESNLSSVLRGFRLFDYNRDRKIQFHELRKILEQYCFPMSNVQFEKLWNSIDYSKMGLIDYMDFLGRLGVTVPNAGSK---------NIGQRT-------ERKKLQLGSSGMN-MDQLEVEYRRRLKEQHGLLKKACLVVDSKHSGLMPIKNFKAILDEFVIAVSDQEFSKLMERFSVKASHDIQWYGFLQKFVDPQSLS-NGQTLPIRPSHKANVVRGSHVEASVQDLIQLLYNHILSAYSSLRNAFLQIDINRKGKITIADFRRLVQGFKFELSEVQIKEIMVQLDPEHTGFITYQKFLDIFEEKESADGHKWLVSTHKYNKDTKPPILAWKTVEDLLHQKIVLRWKSFTAALKHYDSTGTGYINPTNLKKVLDKIAIPLSDEHYDSLLKRLSHKTADGKVNYLEFLAVMQIDVYPGDVEGVSAQIASGNEARLQMRQSQHEKAMADIETHASLRTNMMSLEEIIVRLRDRSSQTRQHIRDAFLQMD-DARKGFVSKAKFRLFLESHGIFLSDENFSQLTRELEF---GREGLDYMRFVKHF-EGRDSE----LNPSYLPMHNHKYNPVRGDEF-GMSAQVVENKLRSKIRENFTTLREAFHKMDSDGRGYLTKQDFRR-LLDQLMCLTNDAEFNKLCKKLGIDDDCKLSYRDFLGKFQEKDDLATGHKWLVSNHRYNE--LGDIKPKTAAEVHQLMAEKVHQNYKDIARAFCHIDYLGRGYITKREFKELLVRLNVTMNQEEFKKLWAYYDTANRNAITHDAFLKRVGVASAFSSGDGQGTSAKIIQNSAEYIAVHLEGQENRQENLVFNQAVLTVGLDADALFIILQKRIRDFFPTTYAAFRALDVKE--KGYLSQEDFRIAVHQMHLFVRDESFAELMRRIGLSQPTAQMTYEAFCLAFDEKFSGPRESQRPKTAGPM-LDGARGGQQQQQQQPQQRQDAKAFLKRLRASVVKDGEILEKVFRSMDTKQEGRIGASDFRRVVDGFCFKLTDAQFKEVVKGL-------LSQGDQVDYYRFLANFSQTQEAAERREWMEAANGGGGGAGGSAEVGESLSQQEVEARVSEFVSARPYLLAQAFAEADYAQLGVLAKTDFRDILFRLVMRMSNEQFDQLMTRVPTNEYGNVDYKEFLSRFASAEQPQQQQQQPTAGGDARPDSPISLTPPASADQAQQQQPQQPTLSLDTEAEAQLRRAMSQSWKSLFSRFKMLDQA 1335
BLAST of EF-hand calcium-binding domain-containing protein 6 vs. TrEMBL
Match: K1R9E2 (EF-hand calcium-binding domain-containing protein 6 OS=Crassostrea gigas OX=29159 GN=CGI_10017631 PE=4 SV=1) HSP 1 Score: 660.218 bits (1702), Expect = 0.000e+0 Identity = 454/1580 (28.73%), Postives = 782/1580 (49.49%), Query Frame = 3
Query: 201 EINPIVKSQLLKNHKEILKALQTFDIECSGLVTKGELRRVLDSYCLPMTTDQYEQFLSHNSINYSKGSINYKELIDKIYDKTSAVGHFKKNIKLPQLQRSATTICLQNKHFTLPTTRRSSGKISRSQLFXXXXXXXXXXXXXXXXWNYNAYTIDDIIPILQMQISRNPNGIMKAFKLFDFNSDYCIQRHRLKKVMENFCCPLTEQQFDKLVARFSQSQNGNVNYMEFMKGMGIPIDNQGTIQTKNSEETI----NIDTRNTKGSRIFEAALIENGLLGSNELEQLLVIFRKKIEDQFSVLRKTFLVMDPNAEGFVTLEILYKILNQFAFPMSLNVFNQIMDKCAFKATGKIIWLSFLQKFQDPQSKHLNGSTIAIKSDHKCNPIQGVNSKPNIDDLLELLSNHISNNYSSFRDAFLQINQTHKGKIPKSEFLKILNRFQFYISADQLSIILKFLDPHNEGYISYHHFLQLFEQKENLSAHKWLNSNHKVVEPRKDLQLSSETIKSTISDNIGNKWSNVVHEFNLVDLNRTGYISVEDLKKIFKKLFGYLKDDDIDRLVAPLTLKD-KKGRINYLELFNLLNVDTSPSDLEGLSTQIAESNDAQLKERHENHIEKMESIEKLGEARLNLMSPEELIVRIKDRIVQNRLNYHELYKKYECNDKKGGINKSQLRKMLTQFGYTLTNEQFSKISEILNFPTSSKSRIDFNGFLAIFADNR-ESSNDLMTDVCFLPKSNHHVNDIRGDDNRNMNCQEVEDLLRQKIVNNFPDLMIAFHKFDRSNKGYLVKSDFQNFLLGNLIMIENDQFDLLCEKLGVGEKSKLTYNDFLNRFQIRDDMVDGHKWLDSKHRFNDPSTNAVPDMSAEDVYMLLPEKIYQYFHEISPTFIALVTSGT-GFIRFRNLQHLLSRLQISMSKSEMKKLWKRYDTNNRNKLDYHDFLYNLGLLEKFGCGDLMGPSAAIIQNSHNALVDHLVNQETKHQQSVDNQAVLGTHLHVEHLFEMVKRHLRVAYSDSALAFKAVANKKTLSGFFTINDLNKLLSQLHMFVTNDKFVQLAARMGFEGPDDSLSYEKFCCIFQDSLNIDGQEKTVGSIRINQDLENPSKIGIEEAGNLNSTQIASIIGKRIVDQYSEIEKTFLVMDPENSGYVSMNEFRNLLVGFCLSLTDKQFNKYRNSLSTYIVKSANTDDKIDYRYFMKGF--------NQKLNNLDIQXXXXXXXXXXXXXTYQQIQEHINESIKVRPKLFLNSFKSKDYASLNFISKSDFREIIERFLFTVPDSHFEKFWS--------NLKKNEF-----------------DNVNYGEFVKMXXXXXXXXXXXXXXXXXXXXXXXXXXNNIETDEMDTD----ESIRKNLERLLNRNWQSIFNKCKAIDQAINECQTAEGSIPIDDFVYILSEYDDSLSAENLA-AINLYKTCKNGRFIKYGDLIRSIVLSSR----GSLISNENVTEVQKKFLNV---GIKTTNSDNSKDYFPQLFKLRRQILENWKDLRKNFLQSDKKQNGAVSGNIFMTIMKTVCGQLSADLFYHLMKIYDQDGTGNFHYKRFIEDFLK 4784
E+ I++ ++ H+ I++A T+D+E + VTKGE RRVL+S+C+P+T++Q+E ++ N S G++ Y + ++K Y + P + + G+ + Q FS + ID I +L+ +IS+N ++KA +LFD+N D IQRH L++V+EN+C LT+ QF KL R+ G V+Y EF+ +G+ +NQ ++ + I N+K IF+ + L +Q+ + FR ++ + L+K FL D + +GF+ LE L IL QF PMS +F Q+M++C K +GKI W FL KFQDPQ NG TI ++++HK PI+ DD+ +LL H+ ++Y S + AFLQI+ T KG+I ++E ++ +F F + +Q ++ LDP++ ISYH FL+LFE+ + HKWLNS H+ E K ++ ETI+ + + I W V D GYIS+ +K I + +D +V+ +D INY+E +LNVD P DL GLS+QI ++ + R +NHI + + + + R N M+ +E+I R+KDR+ Q+++ + + ++ KG + K R++L FG +T++QF +++E + F ++++ F+ F D R + D + V NH VN IRGD+ M EVE LR K+ NF +L AF+KFD + G + K++F+ L + +I +++F+ LC++LG+ + +K++Y +FL RF++RD V+GHKWL+S HR+N ST+ +M+AE + L K ++ + +++ F + G+ G +R R L+ LL ++ I M E K+LW YD + + ++Y DFL LG E F D+MG SA II NSH ++ H +Q KHQ+ +QA+ ++ E + ++ +R ++ D+ AF +K G ++N++ K+LS LH F+ +++F +L ++G L+YE+F F+D + ++ + +RI EE G L+ + + + + I +Q + K F D S +S+ +FR +L F LT+ Q+ ++ L D ++Y F++ + + L+ L Q + ++QE + E++ N FK DYA + ISK DFR+++++ + D F+K W+ NL EF +N +M ++ +S + + ++ M T ES ++ + RNW+ I C+ D+ G+I + + IL + E + Y ++G F Y D +R +L+ + G+L S V + + L+ + + S ++ + +LR +++NWK++R+ F D+ GA+ F +++ L D F+HL+ YD++ G Y FI+ +L+
Sbjct: 302 EVEAILREKVRAKHQNIMQAFMTYDVEHNLGVTKGEFRRVLESFCVPLTSEQFEAVVAKVEKN-SNGTVRYPDFMEKFYGQ--------------------------------PPSSVAGGQWAGYQKFSPPPAA-------------REINIDMIEKMLKDKISQNFKAVVKALQLFDYNRDGKIQRHELRRVIENYCFKLTDDQFQKLWLRYDFHHTGLVSYKEFLSRLGVAANNQNRPPVDGAKGALLWPQQIPVSNSK---IFKQRKDDEEALQRLNFDQIELEFRNRMRKNYLNLKKIFLTFDRHLDGFINLEDLKSILCQFTVPMSDQLFRQMMERCGVKGSGKISWEQFLDKFQDPQGGG-NGQTIPMRNNHKFFPIREGIETVATDDIWKLLYKHVQSHYPSLKQAFLQIDATRKGRITRTELRNVIEKFAFRLDNEQFKQLMLKLDPYHTNSISYHDFLKLFEETDTPEGHKWLNSVHRFNETPKPAIMAWETIEEILREKITEYWKPVSAAIMAADSKGDGYISIPKMKSIIDNCVLPVSEDHFKSMVS--RCQDCSNNMINYVEFLEMLNVDVRPGDLVGLSSQIQSGSEEREAIRVKNHIARATRVNRRAQNRTNTMTADEVITRLKDRMSQHKVQIRQAFLSFD-KSGKGRVTKKNFREVLGSFGVLMTDDQFKELTERVGF---MNGYMNYSDFVMAFEDPRAQGPGDEILRV-----GNHRVNPIRGDE-WGMTADEVEAKLRSKLRENFANLRAAFYKFDDDHNGLINKANFRRILDSFMCIISDNEFEELCKRLGITKSTKISYEEFLQRFEVRD-TVEGHKWLNSVHRYN--STHPTQEMTAESAHEALKLKCHRQWKDLAQAFTQIDAIGSKGVLRRRELRDLLFKMVIPMGPEEFKRLWTFYDEDGKGYINYQDFLDKLGASE-FAPADMMGTSADIIDNSHKTIMVHNEDQLAKHQRMTKHQAIKVAFMNAEQVERQLRDKIRDSFKDTYEAFTKYDTQK--KGSLSVNEIQKVLSDLHFFMDDEQFFKLMDKIGLGTKGSRLNYEEFLRAFEDGRK-EHYQRPLPEVRI------------EECGALSPQEAETKLRRVIENQGDVLGKAFASFDKTQSNLISLADFRRVLDIFAFKLTEPQWRHLQSRLQL-------VDKSVNYVLFLENYAMSDQEDAEKWLSALQKQMRDQSQTLM----PIDEVQERLREAVHAHFYTLANLFKEVDYADIGVISKEDFRDVMDKNVMRFTDEQFDKVWASLPTNDFGNLDYKEFLRRYSSNMEIPPTHRPASTINGRPGTQMSGRRLQSRAMSRSITQMEFGRPGSRLSRAQS-RMSTPLINAESAEARIKNRIFRNWKEIQRSCRNCDR------NNTGTIQVQELKDILVRNGIDMPDEEFYDLMTKYDLREDGSFA-YVDFLRHFILNLKPQDDGNLFSRRRVPQGRVSSLSSVSEDTQVSAGHTSTHFYDAMIRLRECVVQNWKEMRRLFRSMDRDNQGAIPSLDFRYVLRQFSVNLEEDEFFHLLSYYDKNLNGMIAYNDFIKAYLQ 1781 HSP 2 Score: 116.701 bits (291), Expect = 3.615e-22 Identity = 129/569 (22.67%), Postives = 245/569 (43.06%), Query Frame = 3
Query: 2415 NRNMNCQEVEDLLRQKIVNNFPDLMIAFHKFDRSNKGYLVKSDFQNFLLGNLIMIENDQFDLLCEKLGVGEKSKLTYNDFLNRFQIR-DDMVDGHKWLDSKHRFNDPSTNAVPDMSAEDVYMLLPEKIYQYFHEISPTFIALVTSGTGFIRFRNLQHLLSRLQISMSKSEMKKLWKRYDTNNRNKLDYHDFLYNLGLLEK--------FGCGDLMGPSAAIIQNSHNALVDHLVNQETKHQQSVDNQAVLGTHLHVEHLFEMVKRHLRVAYSDSALAFKAVANKKTLSGFFTINDLNKLLSQLHMFVTNDKFVQLAARMGFEGPDDSLSYEKFCCIFQDSLNIDGQEKTVGSIRINQDLENPSKIGIEEAGNLNSTQIASIIGKRIVDQYSEIEKTFLVMDPENSGYVSMNEFRNLLVGFCLSLTDKQFNKYRNSLSTYIVKSANTDDKIDYRYFMKGFNQK--------LNNLDIQXXXXXXXXXXXXXTYQQIQEHINESIKVRPKLFLNSFKSKDYASLNFISKSDFREIIERFLFTVPDSHFEKFWSNLKKNEFDNVNYGEFVKM 4070
N +++ EVE +LR+K+ ++M AF +D + + K +F+ L + + ++QF+ + K+ + Y DF+ +F + V G +W +F+ P A +++ + + +L +KI Q F + + G I+ L+ ++ ++ + +KLW RYD ++ + Y +FL LG+ G L+ P + NS + Q+ D +A+ L+ + + + +R Y + F + + L GF + DL +L Q + +++ F Q+ R G +G +S+E+F FQD G +T+ +R N P + GIE + + I ++ K + Y +++ FL +D G ++ E RN++ F L ++QF + L Y S I Y F+K F + LN++ N + M ++ I+E + E I K + + D +IS + II+ + V + HF+ S + + +NY EF++M
Sbjct: 295 NASLSSIEVEAILREKVRAKHQNIMQAFMTYDVEHNLGVTKGEFRRVLESFCVPLTSEQFEAVVAKVEKNSNGTVRYPDFMEKFYGQPPSSVAGGQWA-GYQKFSPPP--AAREINIDMIEKMLKDKISQNFKAVVKALQLFDYNRDGKIQRHELRRVIENYCFKLTDDQFQKLWLRYDFHHTGLVSYKEFLSRLGVAANNQNRPPVDGAKGALLWPQQIPVSNS-----------KIFKQRKDDEEAL--QRLNFDQIELEFRNRMRKNYLNLKKIF--LTFDRHLDGFINLEDLKSILCQFTVPMSDQLFRQMMERCGVKG-SGKISWEQFLDKFQDPQG-GGNGQTI-PMRNNHKF-FPIREGIE---TVATDDIWKLLYKHVQSHYPSLKQAFLQIDATRKGRITRTELRNVIEKFAFRLDNEQFKQLMLKLDPYHTNS------ISYHDFLKLFEETDTPEGHKWLNSV----HRFNETPKPAIMAWETIEEILREKITEYWKPVSAAIMAADSKGDGYISIPKMKSIIDNCVLPVSEDHFKSMVSRCQDCSNNMINYVEFLEM 828
BLAST of EF-hand calcium-binding domain-containing protein 6 vs. Ensembl Cavefish
Match: ENSAMXT00000042990.1 (EF-hand calcium-binding domain-containing protein 6-like [Source:NCBI gene;Acc:111195861]) HSP 1 Score: 95.5153 bits (236), Expect = 9.172e-21 Identity = 66/269 (24.54%), Postives = 128/269 (47.58%), Query Frame = 3
Query: 180 DPKLQISEINPIVKSQLLKNHKEILKALQTFDIECSGLVTKGELRRVLDSYCLPMTTDQYEQFLSHNSINYSKGSINYKELIDKIYDKTSAVGHFKKNIKLPQLQRSATTICLQNKHFTLPTTRRSSGKISRSQLFXXXXXXXXXXXXXXXXWNYNAYTIDDIIPILQMQISRNPNGIMKAFKLFDFNSDYCIQRHRLKKVMENFCCPLTEQQFDKLVARFSQSQNGNVNYMEFMKGMGIPIDNQGTIQTKNSEETINIDTRN-TKGSR 983
D L ++ I ++ ++ ++ A + FD E VTKGE RRV++ + +P+T Q+ L+ + G+I Y E + + +SA +I++P S+G ++ ++ + ++ L+ +I N I++AF LFD+N D IQ+H L++V+E++C PL++Q+F +L + +S S + ++Y EF++ +G+ +N I +++ +N D N +KG R
Sbjct: 57 DENLSLAHIESMLSQKVRIKKDDLKAAFRGFDQERRNTVTKGEFRRVIEGFLMPLTQSQFNALLAKIP-KTANGAIPYMEFLRQYCRVSSA------SIRIP-----------------------SAG-------------------------SHRSWPLGELQCHLKDKIGGNLKNILRAFCLFDYNRDGRIQKHELRRVLESYCFPLSQQEFHRLWSHYSPSNSHTISYKEFLERLGVDCENYRKIAPDSAKLALNWDAVNQSKGYR 270 HSP 2 Score: 62.7734 bits (151), Expect = 7.849e-10 Identity = 48/189 (25.40%), Postives = 90/189 (47.62%), Query Frame = 3
Query: 3501 NLNSTQIASIIGKRIVDQYSEIEKTFLVMDPENSGYVSMNEFRNLLVGFCLSLTDKQFNKYRNSLSTYIVKSANTDDKIDYRYFMKGFNQKLNNLDIQXXXXXXXXXXXXXTYQQIQEHINESIKVRPKLFLNSFKSKDYASLNFISKSDFREIIERFLFTVPDSHFEKFWSNLKKNEFDNVNYGEFVK 4067
NL+ I S++ +++ + +++ F D E V+ EFR ++ GF + LT QFN +L I K+AN I Y F++ + ++++ I+ SA + ++Q H+ + I K L +F DY I K + R ++E + F + F + WS+ + ++Y EF++
Sbjct: 59 NLSLAHIESMLSQKVRIKKDDLKAAFRGFDQERRNTVTKGEFRRVIEGFLMPLTQSQFN----ALLAKIPKTAN--GAIPYMEFLRQYC-RVSSASIRIPSAGSHRSW---PLGELQCHLKDKIGGNLKNILRAFCLFDYNRDGRIQKHELRRVLESYCFPLSQQEFHRLWSHYSPSNSHTISYKEFLE 237 HSP 3 Score: 60.077 bits (144), Expect = 6.245e-9 Identity = 45/196 (22.96%), Postives = 81/196 (41.33%), Query Frame = 3
Query: 2415 NRNMNCQEVEDLLRQKIVNNFPDLMIAFHKFDRSNKGYLVKSDFQNFLLGNLIMIENDQFDLLCEKLGVGEKSKLTYNDFLNRFQIRDDMVDGHKWLDSKHRFNDPSTNAVPDMSAEDVYMLLPEKIYQYFHEISPTFIALVTSGTGFIRFRNLQHLLSRLQISMSKSEMKKLWKRYDTNNRNKLDYHDFLYNLGL 3002
+ N++ +E +L QK+ DL AF FD+ + + K +F+ + G L+ + QF+ L K+ + Y +FL ++ S PS + ++ L +KI I F + G I+ L+ +L +S+ E +LW Y +N + + Y +FL LG+
Sbjct: 57 DENLSLAHIESMLSQKVRIKKDDLKAAFRGFDQERRNTVTKGEFRRVIEGFLMPLTQSQFNALLAKIPKTANGAIPYMEFLRQY-----------CRVSSASIRIPSAGSHRSWPLGELQCHLKDKIGGNLKNILRAFCLFDYNRDGRIQKHELRRVLESYCFPLSQQEFHRLWSHYSPSNSHTISYKEFLERLGV 241 HSP 4 Score: 52.373 bits (124), Expect = 2.085e-6 Identity = 39/170 (22.94%), Postives = 71/170 (41.76%), Query Frame = 3
Query: 1086 LRKTFLVMDPNAEGFVTLEILYKILNQFAFPMSLNVFNQIMDKCAFKATGKIIWLSFLQKFQDPQSKHLNGSTIAIKSDHKCNPIQGVNSKPNIDDLLELLSNHISNNYSSFRDAFLQINQTHKGKIPKSEFLKILNRFQFYISADQLSIILKFLDPHNEGYISYHHFLQ 1595
L+ F D VT +++ F P++ + FN ++ K A G I ++ FL+++ ++ ++I I P G + + +L L + I N + AF + G+I K E ++L + F +S + + P N ISY FL+
Sbjct: 80 LKAAFRGFDQERRNTVTKGEFRRVIEGFLMPLTQSQFNALLAKIPKTANGAIPYMEFLRQYC-----RVSSASIRI-------PSAGSHRSWPLGELQCHLKDKIGGNLKNILRAFCLFDYNRDGRIQKHELRRVLESYCFPLSQQEFHRLWSHYSPSNSHTISYKEFLE 237
BLAST of EF-hand calcium-binding domain-containing protein 6 vs. Ensembl Cavefish
Match: ENSAMXT00000045911.1 (pep primary_assembly:Astyanax_mexicanus-2.0:24:36828479:36830332:-1 gene:ENSAMXG00000039191.1 transcript:ENSAMXT00000045911.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 50.447 bits (119), Expect = 5.427e-6 Identity = 62/260 (23.85%), Postives = 109/260 (41.92%), Query Frame = 3
Query: 861 NVNYMEFMKGMGIPIDNQGTIQTKNSEETINID--------TRNTKGSRIFEAALIENG--LLGSNELEQLLVIFRKKIEDQFSVLRKTFLVMDPNAEGFVTLE-ILYKILNQFAFPMSLNVFNQIMDKCAFKATGKIIWLSFLQKFQDPQSKHLNGSTIAIKSDHKCNPIQGVNSKPNIDDLLELLSNHISNNYSSFRDAFLQINQTHKGKIPKSEFLKILNRFQFYISADQLSIILKFLDPHNEGYISYHHFLQLFEQ 1607
N+NY EF+K + + + E + + R S I E + + +G L S+ +E+ L R KI + +++ D G + +E L ++ P + F Q+ +K K G++ + FLQ F L + + + + S+ D LL L S I + S + AF+ ++ GKIP EF K+L + +S + + F D + G ISY+ FL +F++
Sbjct: 8 NLNYREFLKKFSGELIEKEKPAAPPTPEHLGLSESSPAVLLQRPKTASCIPERSKVNSGPELSSSSAVERRL---RFKIRSCWREIQRRCKEADTQRTGGIEIEAFLGNVVKMVTTP---SQFEQLSEKYNLKNKGRLSYPEFLQNF----VLMLKPNAPTFTERRRLHLPKTPVSRQCTDALLRLCS-PIQLRWKSIKRAFVFYDKERTGKIPLQEFRKVLRQHNVNLSEEDFFHLASFFDKNISGNISYNEFLCIFQK 256
BLAST of EF-hand calcium-binding domain-containing protein 6 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000004536.1 (pep scaffold:Pmarinus_7.0:GL477109:94389:131012:-1 gene:ENSPMAG00000004110.1 transcript:ENSPMAT00000004536.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 467.233 bits (1201), Expect = 6.061e-139 Identity = 383/1588 (24.12%), Postives = 682/1588 (42.95%), Query Frame = 3
Query: 183 PKLQISEINPIVKSQLLKNHKEILKALQTFDIECSGLVTKGELRRVLDSYCLPMTTDQYEQFLSHNSINYSKGSINYKELIDKIYDKTSAVGHFKKNIKLPQLQRSATTICLQNKHFTLPTTRRSSGKISRSQLFXXXXXXXXXXXXXXXXWNYNAYTIDDIIPILQMQISRNPNGIMKAFKLFDFNSDYCIQRHRLKKVMENFCCPLTEQQFDKLVARFSQSQNGNVNYMEFMKGMGIPIDNQGTIQTKNSEETINI----------DTRNTKGSRIFEAALIENGLLGSNELEQLLVIFRKKIEDQFSVLRKTFLVMDPNAEGFVTLEILYKILNQFAFPMSLNVFNQIMDKCAFKATGKIIWLSFLQKFQDPQSKHLNGSTIAIKSDHKCNPIQGVNSKPNIDDLLELLSNHISNNYSSFRDAFLQINQTHKGKIPKSEFLKILNRFQFYISADQLSIILKFLDPHNEGYISYHHFLQLFEQKENLSAHKWLNSNHKVVEPRKDLQLSSETIKSTISDNIGNKWSNVVHEFNLVDLNRTGYISVEDLKKIFKKLFGYLKDDDIDRLVAPLTLKDKKG-RINYLELFNLLNVDTSPSDLEGLSTQIAESNDAQLKERHENHIEKMESIEKLGEAR--LNLMSPEELIVRIKDRIVQNRLNYHELYKKYECNDKKGGINKSQLRKMLTQFGYTLTNEQFSKISEILNFPTSSKSRIDFNGFLAIFADNRESSNDLMTDVCFLPKSNHHVNDIRGDDNRNMNCQEVEDLLRQKIVNNFPDLMIAFHKFDRSNKGYLVKSDFQNFLLGNLIMIENDQFDLLCEKLGVGEKSKLTYNDFLNRFQIRDDMVDGH-KWLDSKHR---FNDPSTNAVPDMSAEDVYMLLPEKIYQYFHEISPTFIALVTSGTGFIRFRNLQHLLSRLQISMSKSEMKKLW-KRYDTNNRNKLDYHDFLYNLGLLEKFGCGDLMGPSAAIIQNSHNALVDHLVNQETKHQQSVDNQAVLGTHLHVEHLFEMVKRHLRVAYSDSALAFKAVANKKTLSGFFTINDLNKLLSQLHMFVTNDKFVQLAARMGFEGPDDSLSYEKFCCIFQDSLNIDGQEKTVGSIRINQDLENPSK-IGIEEAGNLNSTQIASIIGKRIVDQYSEIEKTFLVMDPENSGYVSMNEFRNLLVGFCLSLTDKQFNKYRNSLSTYIVKSANTDDKIDYRYFMKGF---NQKLNNLDIQXXXXXXXXXX------XXXTYQQIQEHINESIKVRPKLFLNSFKSKDYASLNFISKSDFREIIERFLFTVPDSHFEKFWSNLKKNEFDNV---NYGEFVKMXXXXXXXXXXXXXXXXXXXXXXXXXXNNIETDEMDTD---------------------ESIRKNLERLLNRNWQSIFNKCKAIDQAINECQTAEGSIPIDDFVYILSEYDDSLSAENLAAINLYKTCKNGRFIKYGDLIRSIVLSSRG---SLISNENVTEVQKKFLNVGIKTTNSDNSKDYFPQLFKLRRQILENWKDLRKNFLQSDKKQNGAVSGNIFMTIMKTVCGQLSADLFYHLMKIYDQDGTGNFHYKRFIEDFL 4781
P L SE+ +V+ + + +++++A + D +G V+ GELRRVLD + LP+T Q+ S + G+I Y + ++ HF L Q R +T Q T P R N T D++ L+ ++ R+ G+++ F+LFD+N D +QRH L+ V+E+ L+E QF KL AR Q G V Y EF++ +G+ ++Q + +T+ + T+ + SR +E I N + + R E+ + L++ F D + G + +E +L F P++ +++ + KATGK+ W FL KF +PQS NG T+ ++S H+ NP +G + + ++L L HI Y S ++AFL ++ +G++ + E +++ F F + ++ LDP + G++ Y FL LFE +E L AHKWLNS H+V + L+ + + + D + +W +V F+ D + G+I+ ++L+ I ++ L D+ ++L + G RI + E L VD P D G+S+++ ++A+ +R + ++E IE GEAR + E++VR++D + Q H+ + +++ +G ++ R++L + G + ++QF+ + + L F S I ++ F+A F D R L NH V + +R +C L K+ + DL AF+K D G + D + L + + +F L LG+ E+S+L+Y DFL F +G +WL++ R DPS +M+ E + L + + + ++S F +G ++ L+ LL R + ++ + LW +RYD + + + L L + + G GPSA I + + L Q + + QA G ++E L + +K R YSD AF K GF T +DL +L + + + ++F QL ++G LSY F + V R ++ PSK G L+ + + +++ + ++K F D G V EFR +L FCL L QF L D +D++ F++ F + K D Q A A ++ + + + + R + D + +++ FR+++ R + D FE+ W+ L +E D + +Y EF+K + + + + + I + ++ T E++ L ++R WQ I +C+ D TA G I +D + +L +D LS + LA + + + Y +R +VL+ R L+ + + Q F G ++ + + LR Q+L NW+ +R+ F D +G +S F ++K L+ D F+ ++ YD Y F+ FL
Sbjct: 59 PALSASEVAELVRHGVEERTEQLVRAFRALDPGRAGTVSHGELRRVLDLFVLPLTDPQFGALFSMVPAGRN-GNIPYLQFLE----------HF-----LGQPTRPSTASSRQKS--TPPAGR-----------------------------GINEET--DLL--LRKKLGRDLKGVVRTFRLFDYNGDGRVQRHELRGVLESHGARLSESQFSKLWARHDVHQGGTVAYQEFLESLGLSQESQWHPEP----DTVQLCMLHARHRWEHTQPSCHSRPYEIYEISVKGYCLNIVSTESAVQRIVCEN-YGNLQRAFRAFDGSGSGTLAVEDFVSVLRSFTLPLAPEAMLRVLTQLGIKATGKVPWELFLAKFPNPQSIG-NGQTLPMRSSHRVNPFRGADESTSASEVLARLRKHILECYPSLKEAFLALDINRQGRVTRQELRRLVEGFGFRMGDGHFRELMLLLDPGHSGFVDYTDFLCLFEPRETLDAHKWLNSTHRVNGHKTTDILTWKAAEEILRDKLTERWKDVCEAFDSCDQDADGFITQDNLRAILRRFGPSLSDEHFNKLCT--GCEQGPGFRILFSEFLLRLGVDPRPGDFAGVSSRLHSESNAREVKRQADLSARLELIE--GEARKLTGALPLREVLVRLRDHVAQRDTTLHQSFARHD-RRGEGRVSHFDFRQILQECGMIMEDDQFNALLQRLGFTNGS---ITYSDFVACFDDRRIHGPGEQ----LLLSPNHRVAEASRQHSRAEDCHAA---LPDKLRQVYTDLRSAFNKADGDRDGAVTAGDLRRLLETLHFSLTDGEFGRLLGMLGLNERSRLSYGDFLQTFD--PPTQEGEPRWLEAGARIMEIKDPS-----EMACEQAHQYLVSRARKSWKDVSQAFRDYDENGDRVVQKSELRTLLFRYMLPLTSHDFDSLWARRYDPDGHGVISEQE-LAQLLDVARPPTGVDKGPSARIAEQNQATLATLAEGQRERRCELAGQQARRGAFYNLEQLAQEIKDKFRDKYSDFGAAFARADRDK--DGFVTEDDLRHMLCEHNYSLDREQFEQLLGKLGIPTEGSRLSYADFL-------------RAVDDGRASRYGVRPSKPCGGNATQRLSPEGALAFLREKVPSLLAVLQKAFSAFDKSQRGLVKEEEFRRVLDNFCLKLDGPQFQHLLKRLKR------GGDGTVDWQDFLEAFSLLSDKTEGADWQREVARAALAGHRGAGDHALSAGGVLRRLRDVVSARFYAVARALVCADLPGVGRLTREQFRQVVHRHFLRLSDKQFEELWAQLPVDECDKLDYRDYREFLKRFCDGTALPAAAFTSSAPPALGIYKPPQSLNTGHRPASVAGSVRGGTARAVRSTPLVDCEALESRLRERVSRRWQEILRECRLKD------PTASGEIGANDLLGVLRTFDVWLSEDELAGLASKFAAHSTGHVAYRAFLRHLVLTLRPRQHGLLQRQRLQ--QPSFTPGGTAPNDA-----FLEAMLHLREQVLPNWRPMRRAFRAVDPSASGFLSAPDFRQVLKQFAINLTEDEFFQILNYYDGQLHNRVPYNAFLRTFL 1532
BLAST of EF-hand calcium-binding domain-containing protein 6 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000004547.1 (pep scaffold:Pmarinus_7.0:GL477109:94389:129511:-1 gene:ENSPMAG00000004110.1 transcript:ENSPMAT00000004547.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 401.364 bits (1030), Expect = 2.239e-116 Identity = 360/1598 (22.53%), Postives = 668/1598 (41.80%), Query Frame = 3
Query: 183 PKLQISEINPIVKSQLLKNHKEILKALQTFDIECSGLVTKGELRRVLDSYCLPMTTDQYEQFLSHNSINYSKGSINYKELIDKIYDKTSAVGHFKKNIKLPQLQRSATTICLQNKHFTLPTTRRSSGKISRSQLFXXXXXXXXXXXXXXXXWNYNAYTIDDIIPILQMQISRNPNGIMKAFKLFDFNSDYCIQRHRLKKVMENFCCPLTEQQFDKLVARFSQSQNGNVNYMEFMKGMGIPIDNQGTIQTKNSEETINIDTRNTKGSRIFEAALIENGLLGSNELEQLLVIFRK------------KIEDQFSVLRKTFLVMDPNAEGFVTLEILYKILNQFAFPMSLNVFNQIMDKCAFKATGKIIWLSFLQKFQDPQSKHLNGSTIAIKSDHKCNPIQGVNSKPNIDDLLELLSNHISNNYSSFRDAFLQINQTHKGKIPKSEFLKILNRFQFYISADQLSIILKFLDPHNEGYISYHHFLQLFEQKENLSAHKWLNSNHKVVEPRKDLQLSSETIKSTISDNIGNKWSNVVHEFNLVDLNRTGYISVEDLKKIFKKLFGYLKDDDIDRLVAPLTLKDKKGRINYLELFNLLNVDTSPSDLEGLSTQIAESNDAQLKERHENHIEKMESIEKLGEAR--LNLMSPEELIVRIKDRIVQNRLNYHELYKKYECNDKKGGINKSQLRKMLTQFGYTLTNEQFSKISEILNFPTSSKSRIDFNGFLAIFADNRESSNDLMTDVCFLPKSNHHVNDIRGDDNRNMNCQEVEDLLRQKIVNNFPDLMIAFHKFDRSNKGYLVKSDFQNFLLGNLIMIENDQFDLLCEKLGVGEKSKLTYNDFLNRFQIRDDMVDGH-KWLDSKHR---FNDPSTNAVPDMSAEDVYMLLPEKIYQYFHEISPTFIALVTSGTGFIRFRNLQHLLSRLQISMSKSEMKKLW-KRYDTNNRNKLDYHDFLYNLGLLEKFGCGDLMGPSAAIIQNSHNALVDHLVNQETKHQQSVDNQAVLGTHLHVEHLFEMVKRHLRVAYSDSALAFKAVANKKTLSGFFTINDLNKLLSQLHMFVTNDKFVQLAARMGFEGPDDSLSYEKFCCIFQDSLNIDGQEKTVGSIRINQDLENPSK-IGIEEAGNLNSTQIASIIGKRIVDQYSEIEKTFLVMDPENSGYVSMNEFRNLLVGFCLSLTDKQFNKYRNSLSTYIVKSANTDDKIDYRYFMKGF---NQKLNNLDIQXXXXXXXXXX------XXXTYQQIQEHINESIKVRPKLFLNSFKSKDYASLNFISKSDFREIIERFLFTVPDSHFEKFWSNLKKNEFDNV---NYGEFVKMXXXXXXXXXXXXXXXXXXXXXXXXXXNNIETD--------------EMDTDESIRKNLERLLNRNWQSIFNKCKAID------------QAINECQ----TAEGSIPIDDFVYILSEYDDSLSAENLAAINLYKTCKNGRFIKYGDLIRSIVLSSRG---SLISNENVTEVQKKFLNVGIKTTNSDNSKDYFPQLFKLRRQILENWKDLRKNFLQSDKKQNGAVSGNIFMTIMKTVCGQLSADLFYHLMKIYDQDGTGNFHYKRFIEDFL 4781
P L SE+ +V+ + + +++++A + D +G V+ GELRRVLD + LP+T Q+ + + G+I Y + ++ HF L Q R +T Q T P R N T D++ L+ ++ R+ G+++ F+LFD+N D +QRH L+ V+E+ L+E QF KL AR Q G V Y EF++ +G+ ++Q + + + + ++ F + ++ LG L+ + I ++ ++ + + L++ F D + G + +E +L F P++ +++ + KATGK+ W FL KF +PQS NG T+ ++S H+ NP +G + + ++L L HI Y S ++AFL ++ +G++ + E +++ F F + ++ LDP + G++ Y FL LFE +E L + HK + L+ + + + D + +W +V F+ D + G+I+ ++L+ I ++ L D+ ++L + RI + E L VD P D G+S+++ ++A+ +R + ++E IE GEAR + E++VR++D + Q H+ + +++ +G ++ R++L + G + ++QF+ + + L F S I ++ F+A F D R L NH V + +R +C L K+ + DL AF+K D G + D + L + + +F L LG+ E+S+L+Y DFL F +G +WL++ R DPS +M+ E + L + + + ++S F +G ++ L+ LL R + ++ + LW +RYD + + + L D+ S ++Q N + +++ TK Q + + + + R YSD AF K GF T +DL +L + + + ++F QL ++G LSY F + V R ++ PSK G L+ + + +++ + ++K F D G V EFR +L FCL L QF L D +D++ F++ F + K D Q A A ++ + + + + R + D + +++ FR+++ R + D FE+ W+ L +E D + +Y EF+K + + + + + +T T S+ R + + C+A++ + + EC+ TA G I +D + +L +D LS + LA + + + Y +R +VL+ R L+ + + Q F G ++ + + LR Q+L NW+ +R+ F D +G +S F ++K L+ D F+ ++ YD Y F+ FL
Sbjct: 1 PALSASEVAELVRHGVEERTEQLVRAFRALDPGRAGTVSHGELRRVLDLFVLPLTDPQFGACVIQVPAGRN-GNIPYLQFLE----------HF-----LGQPTRPSTASSRQKS--TPPAGR-----------------------------GINEET--DLL--LRKKLGRDLKGVVRTFRLFDYNGDGRVQRHELRGVLESHGARLSESQFSKLWARHDVHQGGTVAYQEFLESLGLSQESQWHPEPDTVQLCMLHARHRWEHTQPFN--VKDHTRLG---LQSSISIKKQHKVSIHQNSTVLQVCENYGNLQRAFRAFDGSGSGTLAVEDFVSVLRSFTLPLAPEAMLRVLTQLGIKATGKVPWELFLAKFPNPQSIG-NGQTLPMRSSHRVNPFRGADESTSASEVLARLRKHILECYPSLKEAFLALDINRQGRVTRQELRRLVEGFGFRMGDGHFRELMLLLDPGHSGFVDYTDFLCLFEPRETLVGLTHRVNGHKTTD-----ILTWKAAEEILRDKLTERWKDVCEAFDSCDQDADGFITQDNLRAILRRFGPSLSDEHFNKLCTGCE-QGPGFRILFSEFLLRLGVDPRPGDFAGVSSRLHSESNAREVKRQADLSARLELIE--GEARKLTGALPLREVLVRLRDHVAQRDTTLHQSFARHD-RRGEGRVSHFDFRQILQECGMIMEDDQFNALLQRLGFTNGS---ITYSDFVACFDDRRIHGPGEQ----LLLSPNHRVAEASRQHSRAEDCHAA---LPDKLRQVYTDLRSAFNKADGDRDGAVTAGDLRRLLETLHFSLTDGEFGRLLGMLGLNERSRLSYGDFLQTFD--PPTQEGEPRWLEAGARIMEIKDPS-----EMACEQAHQYLVSRARKSWKDVSQAFRDYDENGDRVVQKSELRTLLFRYMLPLTSHDFDSLWARRYDPDGHGVISEQELAQLL---------DVQQSSTCLLQC--NGIYCNVMKGCTKKQCPLIMGRIEEMSFSLSLSSSFHRDKFRDKYSDFGAAFARADRDK--DGFVTEDDLRHMLCEHNYSLDREQFEQLLGKLGIPTEGSRLSYADFL-------------RAVDDGRASRYGVRPSKPCGGNATQRLSPEGALAFLREKVPSLLAVLQKAFSAFDKSQRGLVKEEEFRRVLDNFCLKLDGPQFQHLLKRLKR------GGDGTVDWQDFLEAFSLLSDKTEGADWQREVARAALAGHRGAGDHALSAGGVLRRLRDVVSARFYAVARALVCADLPGVGRLTREQFRQVVHRHFLRLSDKQFEELWAQLPVDECDKLDYRDYREFLKRFCDGTALPAAAFTSSAPPDNGATAMKSRPKTTASCLLTRSEHTHGHAQSTHPSLSGGTARAVR---STPLVDCEALESRLRERVSRRWQEILRECRLKDPTASGEIGANDLLGVLRTFDVWLSEDELAGLASKFAAHSTGHVAYRAFLRHLVLTLRPRQHGLLQRQRLQ--QPSFTPGGTAPNDA-----FLEAMLHLREQVLPNWRPMRRAFRAVDPSASGFLSAPDFRQVLKQFAINLTEDEFFQILNYYDGQLHNRVPYNAFLRTFL 1473
BLAST of EF-hand calcium-binding domain-containing protein 6 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000004557.1 (pep scaffold:Pmarinus_7.0:GL477109:94389:108111:-1 gene:ENSPMAG00000004110.1 transcript:ENSPMAT00000004557.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 117.087 bits (292), Expect = 7.736e-27 Identity = 118/528 (22.35%), Postives = 217/528 (41.10%), Query Frame = 3
Query: 2160 NDKKGGINKSQLRKMLTQFGYTLTNEQFSKISEILNFPTSSKSRIDFNGFLAIFADNRESSNDLMTDVCFLPKSNHHVNDIRGDDNRNMNCQEVEDLLRQKIVNNFPDLMIAFHKFDRSNKGYLVKSDFQNFLLGNLIMIENDQFDLLCEKLGVGEKSKLTYNDFLNRFQIRDDMVDGHKWLDSKHRFNDPSTNAVPD--MSAEDVYMLLPEKIYQYFHEISPTFIALVTSGTGFIRFRNLQHLLSRLQISMSKSEMKKLWKRYDTNNRNKLDYHDFLYNLGLLEKFGCGDLMGPSAAII--------------QNSHNALVDHLVNQETKHQ-QSVDNQAVLGTHLHVEHL----FEMVKRHL--RVAYSDSALAFKAVANKKTLSGFFTINDLNKLLSQLHMFVTNDKFVQLAARMGFEGPDDSLSYEKFCCIFQDSLNIDGQEKTVGSIRINQDLENPSKIGIEEAGNLNSTQIASIIGKRIVDQYSEIEKTFLVMDPENSGYVSMNEFRNLLVGFCLSLTDKQF 3674
DK G + + LR ML + Y+L EQF ++ L PT SR+ + FL D R S + G+ + ++ + LR+K+ + L AF FD+S +G + + +F+ L + ++ QF L ++L G + + DFL F + D +G W R D +SA V L + + F+ ++ + G G + + ++ R + +S + ++LW + + +KLDY D+ L++F C P+AA Q++H +L + T H+ SV GT V E ++ L RV+ + + T SG NDL +L ++++ D+ LA++ ++Y F L + + + G ++ Q L+ PS A N + + ++++ + + + F +DP SG++S +FR +L F ++LT+ +F
Sbjct: 126 RDKDGFVTEDDLRHMLCEHNYSLDREQFEQLLGKLGIPTEG-SRLSYADFLRAVDDGRASRYGVRPS-----------KPCGGNATQRLSPEGALAFLREKVPSLLAVLQKAFSAFDKSQRGLVKEEEFRRVLDNFCLKLDGPQFQHLLKRLKRGGDGTVDWQDFLEAFSLLSDKTEGADWQREVARAALAGHRGAGDHALSAGGVLRRLRDVVSARFYAVARALVCADLPGVGRLTREQFRQVVHRHFLRLSDKQFEELWAQLPVDECDKLDYRDYRE---FLKRF-CDGTALPAAAFTSSAPPGIEHTHGHAQSTHPSLSFPPQSLNTGHRPASVAGSVRGGTARAVRSTPLVDCEALESRLRERVSRRWQEILRECRLKDPTASGEIGANDLLGVLRTFDVWLSEDELAGLASKFAAHS-TGHVAYRAFL----RHLVLTLRPRQHGLLQ-RQRLQQPSFTPGGTAPNDAFLEAMLHLREQVLPNWRPMRRAFRAVDPSASGFLSAPDFRQVLKQFAINLTEDEF 631 HSP 2 Score: 115.161 bits (287), Expect = 3.134e-26 Identity = 136/682 (19.94%), Postives = 255/682 (37.39%), Query Frame = 3
Query: 2874 LQHLLSRLQISMSKSEMKKLWKRYDTNNRNKLDYHDFLYNLGLLEKFGCGDLMGPSAAIIQNSHNALVDHLVNQETKHQQSVDNQAVLGTHLHVEHLFEMVKRHLRVAYSDSALAFKAVANKKTLSGFFTINDLNKLLSQLHMFVTNDKFVQLAARMGFEGPDDSLSYEKFCCIFQDSLNIDGQEKTVGSIRINQDLENPSK-IGIEEAGNLNSTQIASIIGKRIVDQYSEIEKTFLVMDPENSGYVSMNEFRNLLVGFCLSLTDKQFNKYRNSLSTYIVKSANTDDKIDYRYFMKGFN---QKLNNLDIQXXXXXXXXXXX------XXTYQQIQEHINESIKVRPKLFLNSFKSKDYASLNFISKSDFREIIERFLFTVPDSHFEKFWSNLKKNEFDNV---NYGEFVK-------MXXXXXXXXXXXXXXXXXXXXXXXXXXNNIETDEMDTDE-------SIRKNLERLLNRNWQSIFNKCKAID------------QAINECQ----TAEGSIPIDDFVYILSEYDDSLSAENLAAINLYKTCKNGRFIKYGDLIRSIVLSSRG---SLISNENVTEVQKKFLNVGIKTTNSDNSKDYFPQLFKLRRQILENWKDLRKNFLQSDKKQNGAVSGNIFMTIMKTVCGQLSADLFYHLMKIYDQDGTGNFHYKRFIEDFL 4781
L+ LL R + ++ + LW RYD + + + L D+ S ++Q N + +++ TK Q + + + + R YSD AF K GF T +DL +L + + + ++F QL ++G LSY F + V R ++ PSK G L+ + + +++ + ++K F D G V EFR +L FCL L QF L D +D++ F++ F+ K D Q A A ++ + + + + R + D + +++ FR+++ R + D FE+ W+ L +E D + +Y EF+K + + + + + + + + + ++T S+R R + + C+A++ + + EC+ TA G I +D + +L +D LS + LA + + + Y +R +VL+ R L+ + + Q F G ++ + + LR Q+L NW+ +R+ F D +G +S F ++K L+ D F+ ++ YD Y F+ FL
Sbjct: 17 LRTLLFRYMLPLTSHDFDSLWARYDPDGHGVISEQELAQLL---------DVQQSSTCLLQC--NGIYCNVMKGCTKKQCPLIMGRIEEMSFSLSLSSSFHRDKFRDKYSDFGAAFARADRDK--DGFVTEDDLRHMLCEHNYSLDREQFEQLLGKLGIPTEGSRLSYADFL-------------RAVDDGRASRYGVRPSKPCGGNATQRLSPEGALAFLREKVPSLLAVLQKAFSAFDKSQRGLVKEEEFRRVLDNFCLKLDGPQFQHLLKRLKR------GGDGTVDWQDFLEAFSLLSDKTEGADWQREVARAALAGHRGAGDHALSAGGVLRRLRDVVSARFYAVARALVCADLPGVGRLTREQFRQVVHRHFLRLSDKQFEELWAQLPVDECDKLDYRDYREFLKRFCDGTALPAAAFTSSAPPGIEHTHGHAQSTHPSLSFPPQSLNTGHRPASVAGSVRGGTARAVR---STPLVDCEALESRLRERVSRRWQEILRECRLKDPTASGEIGANDLLGVLRTFDVWLSEDELAGLASKFAAHSTGHVAYRAFLRHLVLTLRPRQHGLLQRQRLQ--QPSFTPGGTAPNDA-----FLEAMLHLREQVLPNWRPMRRAFRAVDPSASGFLSAPDFRQVLKQFAINLTEDEFFQILNYYDGQLHNRVPYNAFLRTFL 656 HSP 3 Score: 98.2117 bits (243), Expect = 6.264e-21 Identity = 133/690 (19.28%), Postives = 262/690 (37.97%), Query Frame = 3
Query: 723 FKLFDFNSDYCIQRHRLKKVMENFCCPLTEQQFDKLVARFSQSQNGNVNYMEFMKGMGIPIDNQGTIQTKNSEETINIDTRNTKGSRIFEAALIENGLLGSNE-----LEQLLVIFRKKIEDQFSVLRKTFLVMDPNAEGFVTLEILYKILNQFAFPMSLNVFNQIMDKCAFKATG-KIIWLSFLQKFQDPQSKHLNGSTIAIKSDHKCNPIQGVNSKPNIDDLLELLSNHISNNYSSFRDAFLQINQTHKGKIPKSEFLKILNRFQFYISADQLSIILKFLDPHNEGYISYHHFLQLFE-QKENLSAHKWLNSNHKVV----EPRKDLQLSSETIKSTISDNIGNKWSNVVHEFNLVDLNRTGYISVEDLKKIFKKLFGYLKDDDIDRLVAPLTLKDKKGRINYLELFNLLN-------------VDTSPSDLEGLSTQIAESNDA-QLKERHENHIEKMESIE---KLGEARLNLMSP----EELIVRIKDRIVQNRLNYHELYKKYECND--KKGGINKSQLRKMLTQFGYTLTNEQFSKISEILNFPTSSKSRIDFNGFLA-IFADNRESSNDL-----MTDVCFLPKSNHHVNDIRGDDNRNMNCQEVEDLLRQKIVNNFPDLMIAFHKFDRSNKGYLVKSDFQNFLLGNLIMIENDQFDLLCEKLGVGEKSKLTYNDFLNRFQI 2672
F+ +D N D +Q+ L+ ++ + PLT FD L AR+ +G ++ E + + + + +Q I KG + LI +G E L R K D++S F D + +GFVT + L +L + + + F Q++ K G ++ + FL+ D ++ S ++ C + + + L L + + + + AF +++ +G + + EF ++L+ F + Q +LK L +G + + FL+ F + W + D LS+ + + D + ++ V DL G ++ E +++ + F L D + L A L + D+ +++Y + L ++P +E ++ + + N + S+ + G AR +P E L R+++R+ + + E+ ++ D G I + L +L F L+ ++ + ++ F S + + FL + R + L + F P G N E LR++++ N+ + AF D S G+L DF+ L I + D+F + +++ YN FL F +
Sbjct: 1 FRDYDENGDRVVQKSELRTLLFRYMLPLTSHDFDSLWARYDPDGHGVISEQELAQLLDVQQSSTCLLQCN------GIYCNVMKGCTKKQCPLI----MGRIEEMSFSLSLSSSFHRDKFRDKYSDFGAAFARADRDKDGFVTEDDLRHMLCEHNYSLDREQFEQLLGKLGIPTEGSRLSYADFLRAVDDGRA-----SRYGVRPSKPCG--GNATQRLSPEGALAFLREKVPSLLAVLQKAFSAFDKSQRGLVKEEEFRRVLDNFCLKLDGPQFQHLLKRLKRGGDGTVDWQDFLEAFSLLSDKTEGADWQREVARAALAGHRGAGDHALSAGGVLRRLRDVVSARFYAVARALVCADLPGVGRLTREQFRQVVHRHFLRLSDKQFEELWAQLPV-DECDKLDYRDYREFLKRFCDGTALPAAAFTSSAPPGIEHTHGHAQSTHPSLSFPPQSLNTGHRPASVAGSVRGGTARAVRSTPLVDCEALESRLRERVSR---RWQEILRECRLKDPTASGEIGANDLLGVLRTFDVWLSEDELAGLAS--KFAAHSTGHVAYRAFLRHLVLTLRPRQHGLLQRQRLQQPSFTP----------GGTAPNDAFLEAMLHLREQVLPNWRPMRRAFRAVDPSASGFLSAPDFRQVLKQFAINLTEDEFFQILNYYDGQLHNRVPYNAFLRTFLV 657 HSP 4 Score: 84.3445 bits (207), Expect = 1.038e-16 Identity = 138/695 (19.86%), Postives = 260/695 (37.41%), Query Frame = 3
Query: 2178 INKSQLRKMLTQFGYTLTNEQFSKISEILNFPTSSKSRIDFNGF-------LAIFADNRESSNDLMTDVCFLPKSNHHVNDIRGDDNRN--MNCQEVEDLL----------RQKIVNNFPDLMIAFHKFDRSNKGYLVKSDFQNFLLGNLIMIENDQFDLLCEKLGVG-EKSKLTYNDFLNRFQIRDDMVDGHKWLDSKHRFNDP-STNAVPDMSAEDVYMLLPEKIYQYFHEISPTFIALVTSGTGFIRFRNLQHLLSRLQISMSKSEMKKLWKRYDTNNRNKLDYHDFLYNLGLL-EKFGCGDLMGPSAAIIQNSHNALVDHLVNQETKHQQSVDNQAVLGTHLHVEHLFEMVKRHLR--VAYSDSALAFKAVANKKTLSGFFTINDLNKLLSQLHMFVTNDKFVQLAARMGFEGPD--DSLSYEKFCCIFQDSLNI----------DGQEKTVGSIR------------INQDLENPSKIGIEEAGNLNSTQ---------IASIIGKRIVDQYSEIEKTFLVMDPENSGYVSMNEFRNLLVGFCLSLTDKQFNKYRNSLSTYIVKSANTDDKIDYRYFMKGF--------NQKLNNLDIQXXXXXXXXXXXXXTYQQIQEHINESIKVRPKLFLNSFKSKDYASLNFISKSDFREIIERFLFTVPDSHFEKFWSNLKKNEFDNVNYGEFVK 4067
+ KS+LR +L ++ LT+ F + +R D +G LA D ++SS C L + + N ++G + + +E++ R K + + D AF + DR G++ + D ++ L + ++ +QF+ L KLG+ E S+L+Y DFL VD + R + P NA +S E L EK+ + F A S G ++ + +L + + + + L KR +D+ DFL LL +K D A H DH ++ V R LR V+ A+A V G T +++ + + +++ +F +L A++ + D D Y +F F D + G E T G + +N S G G + + + S + +R+ ++ EI + + DP SG + N+ +L F + L++ + + +A++ + YR F++ + L +Q S P A N + + H+ E + + +F++ D ++ F+S DFR+++++F + + F + + + V Y F++
Sbjct: 12 VQKSELRTLLFRYMLPLTSHDFDSLW----------ARYDPDGHGVISEQELAQLLDVQQSST------CLLQCNGIYCNVMKGCTKKQCPLIMGRIEEMSFSLSLSSSFHRDKFRDKYSDFGAAFARADRDKDGFVTEDDLRHMLCEHNYSLDREQFEQLLGKLGIPTEGSRLSYADFLR-------AVDDGRASRYGVRPSKPCGGNATQRLSPEGALAFLREKVPSLLAVLQKAFSAFDKSQRGLVKEEEFRRVLDNFCLKLDGPQFQHLLKRLKRGGDGTVDWQDFLEAFSLLSDKTEGADWQREVARAALAGHRGAGDHALSAGG------------------------VLRRLRDVVSARFYAVARALVCADLPGVGRLTREQFRQVVHRHFLRLSDKQFEELWAQLPVDECDKLDYRDYREFLKRFCDGTALPAAAFTSSAPPGIEHTHGHAQSTHPSLSFPPQSLNTGHRPASVAGSVRGGTARAVRSTPLVDCEALESRLRERVSRRWQEILRECRLKDPTASGEIGANDLLGVLRTFDVWLSEDELAGLASKF------AAHSTGHVAYRAFLRHLVLTLRPRQHGLLQRQRLQQPSFTPGGTAPNDAFLEAMLHLREQVLPNWRPMRRAFRAVDPSASGFLSAPDFRQVLKQFAINLTEDEFFQILNYYDGQLHNRVPYNAFLR 653 HSP 5 Score: 75.8702 bits (185), Expect = 4.377e-14 Identity = 85/434 (19.59%), Postives = 170/434 (39.17%), Query Frame = 3
Query: 711 IMKAFKLFDFNSDYCIQRHRLKKVMENFCCPLTEQQFDKLVARFSQSQNGNVNYMEFMKGMGIPIDNQGTIQTKNSEETINIDTRNTKGSRIFEAALIENGLLGSNELEQLLVI--FRKKIEDQFSVLRKTFLVMDPNAEGFVTLEILYKILNQFAFPMSLNVFNQIMDKCAFKATGKI---IWLSFLQKFQD--------------PQSKHLNG-----------STIAIKSDHKCNPIQG---------VNSKPNID--DLLELLSNHISNNYSS-FRDAFLQINQTHKGKIPKSEFLKILNRFQFYISADQLSIILKFLDPHNEGYISYHHFLQLFEQKENLSAHKWLNSNHKVVEPRKDLQLSSETIKST------------ISDNIGNKWSNVVHEFNLVDLNRTGYISVEDLKKIFKKLFGYLKDDDI 1850
+ KAF FD + ++ ++V++NFC L QF L+ R + +G V++ +F++ + S++T D + + AAL + G + L V+ R + +F + + + D G +T E +++++ +S F ++ + K+ + FL++F D P +H +G ++ + H+ + G V S P +D L L +S + R+ L+ + T G+I ++ L +L F ++S D+L+ + H+ G+++Y FL+ H L R+ LQ S T T + + + W + F VD + +G++S D +++ K+ L +D+
Sbjct: 222 LQKAFSAFDKSQRGLVKEEEFRRVLDNFCLKLDGPQFQHLLKRLKRGGDGTVDWQDFLEAFSL-----------LSDKTEGADWQRE----VARAALAGHRGAGDHALSAGGVLRRLRDVVSARFYAVARALVCADLPGVGRLTREQFRQVVHRHFLRLSDKQFEELWAQLPVDECDKLDYRDYREFLKRFCDGTALPAAAFTSSAPPGIEHTHGHAQSTHPSLSFPPQSLNTGHRPASVAGSVRGGTARAVRSTPLVDCEALESRLRERVSRRWQEILRECRLK-DPTASGEIGANDLLGVLRTFDVWLSEDELAGLASKFAAHSTGHVAYRAFLRHLVLTLRPRQHGLLQ--------RQRLQQPSFTPGGTAPNDAFLEAMLHLREQVLPNWRPMRRAFRAVDPSASGFLSAPDFRQVLKQFAINLTEDEF 631 HSP 6 Score: 60.4622 bits (145), Expect = 2.545e-9 Identity = 60/348 (17.24%), Postives = 149/348 (42.82%), Query Frame = 3
Query: 249 ILKALQTFDIECSGLVTKGELRRVLDSYCLPMTTDQYEQFLSH------NSINYSKGSINYKELIDKIYDKTSAVGHFKKNIKLPQLQRSATTICLQNKHFTLPTTRRSSGKISRSQLFXXXXXXXXXXXXXXXXWNYNAYTIDDIIPILQMQISRNPNGIMKAFKLFDFNSDYCIQRHRLKKVMENFCCPLTEQQFDKLVARFSQSQNGNVNYMEFMKGMGIPID--NQGTIQTKNSEETINIDTRNTKGSRIFEAALIENGLLGSNELEQLLVIFRKKIEDQFSVLRKTFLVMDPNAEGFVTLEILYKILNQFAFPMSLNVFNQIMDKCAFKATGKIIWLSFLQKF 1268
+ +AL D+ G +T+ + R+V+ + L ++ Q+E+ + + ++Y +Y+E + + D T+ + P ++ + + + P ++G S + S+ + + + + L+ ++SR I++ +L D + I + L V+ F L+E + L ++F+ G+V Y F++ + + + G +Q + R+ + + G ++ + ++ R+++ + +R+ F +DP+A GF++ ++L QFA ++ + F QI++ + ++ + +FL+ F
Sbjct: 334 VARALVCADLPGVGRLTREQFRQVVHRHFLRLSDKQFEELWAQLPVDECDKLDYR----DYREFLKRFCDGTALPAAAFTSSAPPGIEHTHGHAQSTHPSLSFPPQSLNTGHRPAS------VAGSVRGGTARAVRSTPLVDCEALESRLRERVSRRWQEILRECRLKDPTASGEIGANDLLGVLRTFDVWLSEDELAGLASKFAAHSTGHVAYRAFLRHLVLTLRPRQHGLLQRQ----------------RLQQPSFTPGGTAPNDAFLEAMLHLREQVLPNWRPMRRAFRAVDPSASGFLSAPDFRQVLKQFAINLTEDEFFQILNYYDGQLHNRVPYNAFLRTF 655
BLAST of EF-hand calcium-binding domain-containing protein 6 vs. Ensembl Nematostella
Match: EDO28210 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T743]) HSP 1 Score: 205.297 bits (521), Expect = 1.633e-57 Identity = 114/330 (34.55%), Postives = 192/330 (58.18%), Query Frame = 3
Query: 663 DIIPILQMQISRNPNGIMKAFKLFDFNSDYCIQRHRLKKVMENFCCPLTEQQFDKLVARFSQSQNGN-VNYMEFMKGMGIPID-----------NQGTIQTKNSEETINIDTRNTKGSRIFEAALIENGLLGSNELEQLLVIFRKKIEDQFSVLRKTFLVMDPNAEGFVTLEILYKILNQFAFPMSLNVFNQIMDKCAFKATGKIIWLSFLQKFQDPQSKHLNGSTIAIKSDHKCNPIQGVNSKPNIDDLLELLSNHISNNYSSFRDAFLQINQTHKGKIPKSEFLKILNRFQFYISADQLSIILKFLDPHNEGYISYHHFLQLFEQKEN 1616
DI L+ +I+ ++KA +LFD+N D IQRH L++V+ENFC LT +QFD+L ++ ++NG+ ++YM+F+K +G+ NQG T+N + + + R GS + + ++ +LE+ L R K+ + F +++ F+ D +GFVTL+ L +IL F FPMS ++F +M +C +A+ KI + FL+KFQDP+ NG TI IK +HK NP++ P +D+ + L + ++++++S + AFL + G+I + +F +IL F F ++ +Q ++ +DP N GYISY FL FE +E+
Sbjct: 14 DIEQQLRTKIAGQLQAVLKAIRLFDYNRDGQIQRHELRRVVENFCFRLTNEQFDRLWCKYDLTRNGHTLDYMDFLKRLGVNTHPKRRPSTANRLNQGN-GTENKVKFLATNKRPLSGSSSRDGS---PAVMTVKDLERKL---RAKMVENFENIQRAFVAFDRRCDGFVTLDQLKRILTHFVFPMSDSLFEDLMRRCGIRASHKIGYEQFLEKFQDPRVSG-NGQTIPIKPNHKFNPVREAEDMPTTEDIWKFLFSKVTDSFTSVKQAFLVFDDNKDGRITRRDFRRILESFCFRMTEEQFHELMAKIDPMNRGYISYLEFLDRFEHRES 335 HSP 2 Score: 93.9745 bits (232), Expect = 6.088e-20 Identity = 89/389 (22.88%), Postives = 174/389 (44.73%), Query Frame = 3
Query: 168 NYKMDPKLQ-ISEINPIVKSQLLKNHKEILKALQTFDIECSGLVTKGELRRVLDSYCLPMTTDQYEQFLSHNSINYSKGSINYKELIDKIYDKTSAVGHFKKNIKLP-QLQRSATTICLQNKHFTLPTTRRS-SGKISRSQLFXXXXXXXXXXXXXXXXWNYNAYTIDDIIPILQMQISRNPNGIMKAFKLFDFNSDYCIQRHRLKKVMENFCCPLTEQQFDKLVARFSQSQNGNVNYMEFMKGMGIPIDNQGTIQTKNSEETINIDTRNTKGSRIFEAALIENGLLGSNELEQLLVIFRKKIEDQFSVLRKTFLVMDPNAEGFVTLEILYKILNQFAFPMSLNVFNQIMDKCAFKATGKIIWLSFLQKFQDPQSKHLNGSTIAIKSDH 1325
N PK Q ++I +++++ + +LKA++ FD G + + ELRRV++++C +T +Q+++ + + +++Y + + ++ T H K+ +L + T +NK L T +R SG SR + T+ D+ L+ ++ N I +AF FD D + +LK+++ +F P+++ F+ L+ R + + Y +F++ P + + +TI I N K + + EA + E + K+ D F+ +++ FLV D N +G +T +IL F F M+ F+++M K G I +L FL +F+ +S L+ + + D
Sbjct: 2 NQTKSPKEQSFADIEQQLRTKIAGQLQAVLKAIRLFDYNRDGQIQRHELRRVVENFCFRLTNEQFDRLWCKYDLTRNGHTLDYMDFLKRLGVNT----HPKRRPSTANRLNQGNGT---ENKVKFLATNKRPLSGSSSRD-------------------GSPAVMTVKDLERKLRAKMVENFENIQRAFVAFDRRCDGFVTLDQLKRILTHFVFPMSDSLFEDLMRRCGIRASHKIGYEQFLEKFQDP-------RVSGNGQTIPIKP-NHKFNPVREAEDMPT-------TEDIWKFLFSKVTDSFTSVKQAFLVFDDNKDGRITRRDFRRILESFCFRMTEEQFHELMAKIDPMNRGYISYLEFLDRFEHRESPVLDSLGLYMTDDQ 349 HSP 3 Score: 87.0409 bits (214), Expect = 1.127e-17 Identity = 73/333 (21.92%), Postives = 147/333 (44.14%), Query Frame = 3
Query: 1695 IKSTISDNIGNKWSNVVHEFNLVDLNRTGYISVEDLKKIFKKLFGYLKDDDIDRLVAPLTLKDKKGRINYLELFNLLNVDTSPSDLEGLSTQIAESNDAQLKERHENHIEKMESIEKLGEARLNLMSPEELIVRIKDRIVQNRLNYHELYKKYE--CNDKKGGINKSQLRKMLTQFGYTLTNEQFSKISEILNFPTSSKSRIDFNGFLAIFADNRESSNDLMTDVCFLPKSNHHVNDIRGDDNRNMNCQEVEDLLRQKIVNNFPDLMIAFHKFDRSNKGYLVKSDFQNFLLGNLIMIENDQFDLLCEKLGVGEKSKLTYNDFLNRFQIRDDMV 2687
I+ + I + V+ L D NR G I +L+++ + L ++ DRL L ++Y++ L V+T P + ++ + N + K + ++ S + +M+ ++L +++ ++V+N N + ++ C+ G + QL+++LT F + +++ F + S K I + FL F D R S N + K NH N +R ++ +++ L K+ ++F + AF FD + G + + DF+ L + +QF L K+ + ++Y +FL+RF+ R+ V
Sbjct: 15 IEQQLRTKIAGQLQAVLKAIRLFDYNRDGQIQRHELRRVVENFCFRLTNEQFDRLWCKYDLTRNGHTLDYMDFLKRLGVNTHPKRRPSTANRLNQGNGTENKVKFLATNKRPLSGSSSRDGSPAVMTVKDLERKLRAKMVENFENIQRAFVAFDRRCD---GFVTLDQLKRILTHFVFPMSDSLFEDLMRRCGIRASHK--IGYEQFLEKFQDPRVSGNGQTIPI----KPNHKFNPVREAEDMP-TTEDIWKFLFSKVTDSFTSVKQAFLVFDDNKDGRITRRDFRRILESFCFRMTEEQFHELMAKIDPMNRGYISYLEFLDRFEHRESPV 337 HSP 4 Score: 83.1889 bits (204), Expect = 1.930e-16 Identity = 73/292 (25.00%), Postives = 128/292 (43.84%), Query Frame = 3
Query: 2163 DKKGGINKSQLRKMLTQFGYTLTNEQFSKISEILNFPTSSKSRIDFNGFLAIFADN-----RESS-------NDLMTDVCFLPKSNHHVNDIRGDDNRN--MNCQEVEDLLRQKIVNNFPDLMIAFHKFDRSNKGYLVKSDFQNFLLGNLIMIENDQFDLLCEKLGVGEKSKLTYNDFLNRFQIRDDMVDGHKW---LDSKHRFNDPSTNAVPDMSAEDVYMLLPEKIYQYFHEISPTFIALVTSGTGFIRFRNLQHLLSRLQISMSKSEMKKLWKRYDTNNRNKLDYHDFL 2987
++ G I + +LR+++ F + LTNEQF ++ + T + +D+ FL N R S+ N V FL + ++ D M +++E LR K+V NF ++ AF FDR G++ + L + + + F+ L + G+ K+ Y FL +FQ D V G+ + H+FN P A + ED++ L K+ F + F+ + G I R+ + +L M++ + +L + D NR + Y +FL
Sbjct: 40 NRDGQIQRHELRRVVENFCFRLTNEQFDRLWCKYDL-TRNGHTLDYMDFLKRLGVNTHPKRRPSTANRLNQGNGTENKVKFLATNKRPLSGSSSRDGSPAVMTVKDLERKLRAKMVENFENIQRAFVAFDRRCDGFVTLDQLKRILTHFVFPMSDSLFEDLMRRCGIRASHKIGYEQFLEKFQ--DPRVSGNGQTIPIKPNHKFN-PVREAEDMPTTEDIWKFLFSKVTDSFTSVKQAFLVFDDNKDGRITRRDFRRILESFCFRMTEEQFHELMAKIDPMNRGYISYLEFL 327 HSP 5 Score: 80.1073 bits (196), Expect = 2.019e-15 Identity = 64/289 (22.15%), Postives = 122/289 (42.21%), Query Frame = 3
Query: 66 DITDSLIVRKSIYREPIQSNSLNQ----------------PEKNSTIKDANYKMDPKLQISEINPIVKSQLLKNHKEILKALQTFDIECSGLVTKGELRRVLDSYCLPMTTDQYEQFLSHNSINYSKGSINYKELIDKIYDKTSAVGHFKKNIKLPQLQRSATTICLQNKHFTLPTTRRSSGKISRSQLFXXXXXXXXXXXXXXXXWNYNAYTIDDIIPILQMQISRNPNGIMKAFKLFDFNSDYCIQRHRLKKVMENFCCPLTEQQFDKLVARFSQSQNGNVNYMEFM 884
D L V R P +N LNQ P S+ +D + + + + ++ +++++++N + I +A FD C G VT +L+R+L + PM+ +E + I S I Y++ ++K D P++ + TI ++ H P R + + T +DI L +++ + + +AF +FD N D I R ++++E+FC +TE+QF +L+A+ G ++Y+EF+
Sbjct: 86 DFLKRLGVNTHPKRRPSTANRLNQGNGTENKVKFLATNKRPLSGSSSRDGSPAV---MTVKDLERKLRAKMVENFENIQRAFVAFDRRCDGFVTLDQLKRILTHFVFPMSDSLFEDLMRRCGIRASH-KIGYEQFLEKFQD--------------PRVSGNGQTIPIKPNHKFNPV--REAEDMP---------------------------TTEDIWKFLFSKVTDSFTSVKQAFLVFDDNKDGRITRRDFRRILESFCFRMTEEQFHELMAKIDPMNRGYISYLEFL 327 HSP 6 Score: 79.337 bits (194), Expect = 3.383e-15 Identity = 69/280 (24.64%), Postives = 130/280 (46.43%), Query Frame = 3
Query: 2853 GFIRFRNLQHLLSRLQISMSKSEMKKLWKRYD-TNNRNKLDYHDFLYNLGLLEKFGCGDLMGPSAAIIQNSHNALVDHLVNQETKHQQSVDNQAVLGTH--LHVEHLFEMVKRHLRVAYSDSALAFKAVANKKTLSGFFTINDLNKLLSQLHMFVTNDKFVQLAARMGFEGPDDSLSYEKFCCIFQDSLNIDGQEKTVGSIRINQDLENPSKIGIEEAGNLNSTQ-IASIIGKRIVDQYSEIEKTFLVMDPENSGYVSMNEFRNLLVGFCLSLTDKQFNK 3680
G I+ L+ ++ ++ + +LW +YD T N + LDY DFL LG+ PS A N N + + T + + + G+ + V+ L ++ + + + AF VA + GF T++ L ++L+ +++ F L R G + YE+F FQD + G +T+ I+ N NP + EA ++ +T+ I + ++ D ++ +++ FLV D G ++ +FR +L FC +T++QF++
Sbjct: 43 GQIQRHELRRVVENFCFRLTNEQFDRLWCKYDLTRNGHTLDYMDFLKRLGV----NTHPKRRPSTANRLNQGNGTENKVKFLATNKRPLSGSSSRDGSPAVMTVKDLERKLRAKMVENFENIQRAF--VAFDRRCDGFVTLDQLKRILTHFVFPMSDSLFEDLMRRCGIRA-SHKIGYEQFLEKFQDP-RVSGNGQTI-PIKPNHKF-NP----VREAEDMPTTEDIWKFLFSKVTDSFTSVKQAFLVFDDNKDGRITRRDFRRILESFCFRMTEEQFHE 308
BLAST of EF-hand calcium-binding domain-containing protein 6 vs. Ensembl Nematostella
Match: EDO46548 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RPQ2]) HSP 1 Score: 86.6557 bits (213), Expect = 6.319e-17 Identity = 107/465 (23.01%), Postives = 196/465 (42.15%), Query Frame = 3
Query: 2403 RGDDNRNMNCQEVEDLLRQKIVNNFPDLMIAFHKFDRSNKGYLVKSDFQNFL---LGNLIMIENDQFDLLCEKLGVGEKSKLTYNDFLNRFQIRDDMVDGHKWLDSKHRFNDPSTNAVPDMSAEDVYMLLPEKIYQYFHEISPTFIALVTSGTGFIRFRNLQHLLSRLQISMSKSEMKKLWKRYDTNNRNKLDYHDFLYNLGLLEKFGCGDLMGPSAAIIQNSHNALVDHLVNQETKHQQSVDNQAVLGTHLHVEHLFEMVKRHLRVAYSDSALAFKAVANKKTLSGFFTINDLNKLLSQLHMFVTNDKFVQ-LAARMGFE-GPDDSLSYEKFCCIFQDSLNIDGQEKTVGSIRINQDLENPSKIGIEEAGNLNSTQIASIIGKRIVDQYSE----IEKTFLVMDPENSGYVSMNEFRNLLVG-FCLSLTDKQFNKYRNSLSTYIVKSANTDDKIDYRYFMKGFN 3767
RG D +++ E E LLR+K+ + + F + D NKG + + L +G I + QF+ L +L + K + Y++F F+ +G WL+ P+ M+A V+ L EK Q F +++ + G+G I L+++L+++ M E +KLW +YDT N + + LG+ K G M S ++ +D + +KR R +SD AF + +T G +D K+L++ + ++ DK V+ AR G DD + Y++F FQD + DG + + +++ + + G+ ST S + R++D + + TF +D ++ G ++ +FR + G F L ++D+++ + + N D + Y FM F+
Sbjct: 126 RGPDKASVD--EFEYLLREKMKTGYFTIKNLFKQRDPENKGIVSRESLSQILTTFVGKPISLS--QFNKLMVRLNLDHKKMIAYDEFYAAFRPPKKADEGPAWLE----LEKPNMRT---MTASQVHAHLKEKAKQRFLDVAELIPQMNPGGSGRILRPELRNVLNQMGFYMDDDEFEKLWLKYDTENVGTIRAEKLMGKLGISIKPGSTGDMPRSPRKLEVERKVQLD---------------------------IERWLKRKFREGFSDMKHAFMELDLDRT--GRVRKDDFRKVLAEFDLKLSTDKQVEDFLARCGVTLTSDDLVPYKEFLRRFQDR-SQDGMPHKILANQLH-------RYHHSDQGSQFST--TSAVEARLMDLFQRDFLALLGTFHNIDRKDCGLLTQQQFRAAIEGRFELGMSDEEYEDFLTRVPI------NNDGMVKYVEFMSKFD 534 HSP 2 Score: 74.7146 bits (182), Expect = 2.498e-13 Identity = 88/469 (18.76%), Postives = 194/469 (41.36%), Query Frame = 3
Query: 240 HKEILKALQTF---DIECSGLVTKGELRRVLDS-YCLPMTTDQYEQFLSHNSINYSKGSINYKELIDK-----------IYDKTSAVGHFKKNIKLPQLQRSATTICLQNKHFTLPTTRRSSGKISRSQLFXXXXXXXXXXXXXXXXWNYNAYTIDDIIPILQMQISRNPNGIMKAFKLFDFNSDYCIQRHRLKKVMENFCCPLTEQQFDKLVARFSQSQNGNVNYMEFMKGMGIPIDNQGTIQTKNSEETINIDTRNTKGSRIFEAALIENGLLGSNELEQLLVIFRKKIEDQFSVLRKTFLVMDPNAEGFVTLEILYKILNQFAFPMSLNVFNQIMDKCAFKATGKIIWLSFLQKFQDPQSKHLNGSTIAIKSDHKCN-PIQGVNSKPN--IDDLLELLSNHISNNYSSFRDAFLQINQTHKGKIPKSEFLKILNRFQFYISADQLSIILKFLDPHNEGYISYHHFL 1592
++ L L TF D + GL+T+ + R ++ + L M+ ++YE FL+ IN + G + Y E + K ++D++S +G + +P ++ + KH R + + + + + +++ +++ + N +AF D + + ++K+++ F + + KL F Q G++ Y++F++ G I + K S +G F ++ + L S+ ++ L + K++ + LR F DPN GFV+ E +IL ++ + + +K K ++ ++ FL+ + + ++ + + + + + P+ + KPN + + L +S + + R AF +++ + G + EF +L + D++ +L LD G ++Y FL
Sbjct: 466 QRDFLALLGTFHNIDRKDCGLLTQQQFRAAIEGRFELGMSDEEYEDFLTRVPIN-NDGMVKYVEFMSKFDTTFDRDNNSLFDRSSVLGAPEP---MPPIREKESPPGSPRKH-------RRPRPVKTVDSYD----------------SQDGRSAEELKRVIKNVLVNNFQDFEQAFYDLDELNSKRLTPEMMRKLLKKFDVDVNRNEIKKLWDTFITDQRGHLEYLQFVRHFGYSIGSAAFPNAKVSPPK--------RGDNDF---MMRSNKLNSD-VDMLFDQLKSKVDFHWDRLRGEFAAADPNNAGFVSREAFKEILQDLCIELTDYELSYVTNKFDPKNENRVSYVKFLEPYAKRRRRYFSNNMGTVMNHPQAELPMDDIVPKPNKGLSGITAKLRQKLSGEWKNLRKAFRKLDLQNDGYLSIPEFRSVLRLCNTVLEEDEIYHLLSELDSGMSGNVNYREFL 895 HSP 3 Score: 72.7886 bits (177), Expect = 8.998e-13 Identity = 135/679 (19.88%), Postives = 278/679 (40.94%), Query Frame = 3
Query: 756 IQRHRLKKVMENFCCPLTEQQFDKLVARFSQSQNGNVNYMEFMKGMGIPIDNQGTIQTKNSEETINIDTRNTKGSRIFEAALIENGLLGSNELEQLLVIFRKKIEDQFSVLRKTFLVMDPNAEGFVTLEILYKILNQFAFPMSLNVFNQIMD---KCAFKATGK--IIWLSFLQKFQDPQSKHLNGSTIAIKSDHKCNPIQGVNSKPNIDDLLEL-LSNHISNNYSSFRDAFLQINQTHKGKIPKSEF-LKILNRFQFYISADQLSIILKFLDPHNEGYISYHHFLQ------------LFEQKENLSAHKWLNS------------NHKVVEPRKDLQ-------LSSETIKSTISDNIGNKWSNVVHEFNLVDLNRTGYISVEDLKKIFKKLFGYLKDDDIDRLVAPLTLKDKKGRINYLELFNLLNVDTSPSDLEGLSTQIAESNDAQLKERHENHIEKMESIEKLGEARLNLMSPEELIVRIKDRIVQNRLNYHELYKKYECNDK--KGGINKSQLRKMLTQFGYTLTNEQFSKISEILNFPTSSKSRIDFNGFLAIFADNRES--SNDLMTDVCFLPKSNHHVNDIRGDDNRNMNCQEVEDLLRQKIVNNFPDLMIAFHKFDRSNKGYLVKSDFQNFL-LGNLIMIENDQFDLLCEKLGVGEKSKLTYNDFLNR 2663
I R L+ V+ + + +F+KL ++ G + + M +GI I T S + ++ + ++E+ L ++K + FS ++ F+ +D + G V + K+L +F +S + Q+ D +C T + + FL++FQD + +A + + QG S+ + +E L + ++ + F I++ G + + +F I RF+ +S ++ L + +N+G + Y F+ LF++ L A + + H+ P K + S+E +K I + + N + + F +D + ++ E ++K+ KK + ++I +L + D++G + YL+ G S A +A++ + M KL + L ++K ++ ++ L ++ D G +++ +++L LT+ + S ++ F +++R+ + FL +A R SN++ T V P++ ++DI N+ ++ + LRQK+ + +L AF K D N GYL +F++ L L N ++ E++ + LL E L G + Y +FL +
Sbjct: 270 ILRPELRNVLNQMGFYMDDDEFEKLWLKYDTENVGTIRAEKLMGKLGISIKPGSTGDMPRSPRKLEVERKVQL------------------DIERWL---KRKFREGFSDMKHAFMELDLDRTGRVRKDDFRKVLAEFDLKLSTD--KQVEDFLARCGVTLTSDDLVPYKEFLRRFQDRSQDGMPHKILANQLHRYHHSDQG--SQFSTTSAVEARLMDLFQRDFLALLGTFHNIDRKDCGLLTQQQFRAAIEGRFELGMSDEEYEDFLTRVPINNDGMVKYVEFMSKFDTTFDRDNNSLFDRSSVLGAPEPMPPIREKESPPGSPRKHRRPRPVKTVDSYDSQDGRSAEELKRVIKNVLVNNFQDFEQAFYDLDELNSKRLTPEMMRKLLKKFDVDVNRNEIKKLWDTF-ITDQRGHLEYLQFVRHF----------GYSIGSAAFPNAKVSPPKRGDNDFMMRSNKLNS------DVDMLFDQLKSKV---DFHWDRLRGEFAAADPNNAGFVSREAFKEILQDLCIELTDYELSYVTN--KFDPKNENRVSYVKFLEPYAKRRRRYFSNNMGT-VMNHPQAELPMDDIVPKPNKGLSG--ITAKLRQKLSGEWKNLRKAFRKLDLQNDGYLSIPEFRSVLRLCNTVLEEDEIYHLLSE-LDSGMSGNVNYREFLTK 897 HSP 4 Score: 65.4698 bits (158), Expect = 1.806e-10 Identity = 148/796 (18.59%), Postives = 316/796 (39.70%), Query Frame = 3
Query: 1356 KPNIDDLLELLSNHISNNYSSFRDAFLQINQTHKGKIPKSEFLKILNRF-QFYISADQLSIILKFLDPHNEGYISYHHFLQLFEQ-KENLSAHKWLNSNHKVVEPRKDLQLSSETIKSTISDNIGNKWSNVVHEFNLVDLNRTGYISVEDLKKIFKKLFGYLKDDDIDRLVAPLTLKDKKGRINYLELFNLLNVDTSPSDLEGLSTQIAESNDAQLKERHENHIEKMESIEKLGEARLNLMSPEELIVRIKDRIVQNRLNYHELYKKYECNDKKGGINKSQLRKMLTQFGYTL-TNEQFSKISEILNFPTSSKSRIDFNGFLAIFADNRESSNDLMTDVCFLPKSNHHVNDIRGDDNRNMNCQEVEDLLRQKIVNNFPDLMIAFHKFDRSNKGYLVKSDFQNFLLGNL-IMIENDQFDLLCEKLGVGEKSKLTYNDFLNRFQI---RDD--MVDGHKWLDS------------------KHRFNDP-----STNAVPDMSAEDVYMLLPEKIYQYFHEISPTFIALVTSGTGFIRFRNLQHLLSRLQISMSKSEMKKLWKRYDTNNRNKLDYHDFLYNLGLLEKFGCGDLMGPSAAIIQNSHNALVDHLVNQETKHQQSVDNQAVLGTH---LHVEHLFEMVKRHLRVAYSDSALAFKAVANKKTLSGFFTINDLNKLLSQLHMFVTNDKFVQLAARMGFEGPDDSLSYEKFCCIFQDSLNIDGQEKTVGSIRINQDLENPSKIGIEEAGNLNSTQIASIIGKRIVDQYSEIEKTFLVMDPENSGYVSMNEFRNLL 3638
K ++D+ LL + Y + ++ F Q + +KG + + +IL F IS Q + ++ L+ ++ I+Y F F K+ WL +E +++ + + + + ++ +V ++ +G I +L+ + ++ Y+ DD+ ++L ++ G I +L L G+S + + D R K+E E ++ L L + ++ + + EL D+ G + K RK+L +F L T++Q +S + + FL F D S D M + + + + +G ++ VE L +F L+ FH DR + G L + F+ + G + + +++++ ++ + + Y +F+++F RD+ + D L + KHR P S ++ SAE++ ++ + F + F L + + ++ LL + + ++++E+KKLW + T+ R L+Y F+ + G + G P+A + DN ++ ++ V+ LF+ +K +V + L + A +GF + ++L L + +T+ + + + + ++ +SY KF + +G++ + E P I N + I + + +++ ++ + K F +D +N GY+S+ EFR++L
Sbjct: 130 KASVDEFEYLLREKMKTGYFTIKNLFKQRDPENKGIVSRESLSQILTTFVGKPISLSQFNKLMVRLNLDHKKMIAYDEFYAAFRPPKKADEGPAWLE-----LEKPNMRTMTASQVHAHLKEKAKQRFLDVAELIPQMNPGGSGRILRPELRNVLNQMGFYMDDDEFEKLWLKYDTENV-GTIRAEKLMGKL----------GISIKPGSTGDMPRSPR------KLEV-----ERKVQLDIERWLKRKFREGFSDMKHAFMEL-----DLDRTGRVRKDDFRKVLAEFDLKLSTDKQVEDFLARCGVTLTSDDLVPYKEFLRRFQDR---SQDGMPHKILANQLHRYHHSDQG--SQFSTTSAVEARLMDLFQRDFLALLGTFHNIDRKDCGLLTQQQFRAAIEGRFELGMSDEEYEDFLTRVPINNDGMVKYVEFMSKFDTTFDRDNNSLFDRSSVLGAPEPMPPIREKESPPGSPRKHRRPRPVKTVDSYDSQDGRSAEELKRVIKNVLVNNFQDFEQAFYDLDELNSKRLTPEMMRKLLKKFDVDVNRNEIKKLWDTFITDQRGHLEYLQFVRHFG----YSIGSAAFPNAKVSPPKRG-----------------DNDFMMRSNKLNSDVDMLFDQLKS--KVDFHWDRLRGEFAAADPNNAGFVSREAFKEILQDLCIELTDYELSYVTNKFDPKN-ENRVSYVKFLEPYAKRRR-RYFSNNMGTVMNHPQAELPMD-DIVPKPNKGLSGITAKLRQKLSGEWKNLRKAFRKLDLQNDGYLSIPEFRSVL 862 HSP 5 Score: 58.5362 bits (140), Expect = 2.013e-8 Identity = 132/690 (19.13%), Postives = 275/690 (39.86%), Query Frame = 3
Query: 711 IMKAFKLFDFNSDYCIQRHRLKKVMENFCC-PLTEQQFDKLVARFSQSQNGNVNYMEFMKGMGIPIDNQGTIQTKNSEETINIDTRNTKGSRIFEAALIENGLLGSNELEQLLVIFRKKIEDQFSVLRKTFLVMDPNAEGFVTLEILYKILNQFAFPMSLNVFNQIMDKCAFKATGKIIWLSFLQKFQDPQSKHLNGSTIAIKS--DHKCNPIQ-GVNSKPNIDDLLELLSNHISNNYSSFRDAFLQINQTHKGKIPKSEFLKILNRFQFYISAD-QLSIILK--FLDPHNEGYISYHHFLQLFEQK-ENLSAHKWL-NSNHKVVEPRKDLQLSSET-IKSTISDNIGNKWSNVVHEFNLVDLNRTGYISVEDLKKIFKKLFGY-LKDDDIDRLVAPLTLKDKKGRINYLE---------------LFNLLNVDTSPSDLEGLSTQIAESNDAQLKERHENHIEKMESIEKLGEARLNLMSPEELIVRIKDRIVQNRLNYHE-LYKKYECNDKKGGINKSQLRKMLTQFGYTLTNEQFSKISEILNFPTSSKSRIDFNGFLAIFA--------DNRESSNDLMTDVCFLPKSNHHVNDIRGDDNRNMNCQEVEDLLRQKIVNNFPDLMIAFHKFDRSNKGYLVKSDFQNFLLGNLIMIENDQFDLLCEKLGVGEKSKLTYNDFLNRFQIR 2675
I FK D + + R L +++ F P++ QF+KL+ R + + Y EF P K ++E G E +E + + Q+ ++K + +F + + M+P G + L +LNQ F M + F ++ K + G I + K G +I S D +P + V K +D + L +S + AF++++ G++ K +F K+L F +S D Q+ L + ++ + Y FL+ F+ + ++ HK L N H+ + Q S+ + +++ + D + ++ F+ +D G ++ + + + F + D++ + + + + + G + Y+E LF+ +V +P + + + + + K R ++ ++S + + S EEL IK+ +V N ++ + Y E N K+ + +RK+L +F + + K+ + F T + +++ F+ F N + S D F+ +SN +D+ + D L+ K+ ++ L F D +N G++ + F+ L I + + + + K +++++Y FL + R
Sbjct: 151 IKNLFKQRDPENKGIVSRESLSQILTTFVGKPISLSQFNKLMVRLNLDHKKMIAYDEFYAAFRPP---------KKADE----------GPAWLE---LEKPNMRTMTASQVHAHLKEKAKQRFLDVAELIPQMNPGGSGRILRPELRNVLNQMGFYMDDDEFEKLWLKYDTENVGTIRAEKLMGKL---------GISIKPGSTGDMPRSPRKLEVERKVQLD-IERWLKRKFREGFSDMKHAFMELDLDRTGRVRKDDFRKVLAEFDLKLSTDKQVEDFLARCGVTLTSDDLVPYKEFLRRFQDRSQDGMPHKILANQLHRYHHSDQGSQFSTTSAVEARLMDLFQRDFLALLGTFHNIDRKDCGLLTQQQFRAAIEGRFELGMSDEEYEDFLTRVPI-NNDGMVKYVEFMSKFDTTFDRDNNSLFDRSSVLGAPEPMPPIREKESPPGSPR-KHRRPRPVKTVDSYDS-----QDGRSAEELKRVIKNVLVNNFQDFEQAFYDLDELNSKR--LTPEMMRKLLKKFDVDVNRNEIKKLWD--TFITDQRGHLEYLQFVRHFGYSIGSAAFPNAKVSPPKRGDNDFMMRSNKLNSDV----------DMLFDQLKSKVDFHWDRLRGEFAAADPNNAGFVSREAFKEILQDLCIELTDYELSYVTNKFDPKNENRVSYVKFLEPYAKR 787
BLAST of EF-hand calcium-binding domain-containing protein 6 vs. Ensembl Nematostella
Match: EDO32038 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SW85]) HSP 1 Score: 63.5438 bits (153), Expect = 2.689e-10 Identity = 50/214 (23.36%), Postives = 95/214 (44.39%), Query Frame = 3
Query: 2373 PKSNHHVNDIRGDDNRNMNCQEVEDLLRQKIVNNFPDLMIAFHKFDRSNKGYLVKSDFQNFLLGNLIMIENDQFDLLCEKLGVGEKSKLTYNDFLNRFQIRDDMVDGHKWLDSKHRFNDPSTNAVPDMSAEDVYMLLPEKIYQYFHEISPTFIALVTSGTGFIR-FRNLQHLLSRLQISMSKSEMKKLWKRYDTNNRNKLDYHDFLYNLGLLEK 3011
P+ HH R M+ EV+ +++ ++ F L AF D S G + + L ++ + +DQFD + + L ++ Y +FL + RDD++ + + PS ++ +S M+ P K Y SP + S + R +N +L + + ++ +E+ LW+++D N + ++ Y DFL + L K
Sbjct: 56 PEGYHH--------RRTMSEDEVKQRVKEVVMARFYRLAKAFTDQDSSRSGTVSPRGLRRVLDEHVFRMNDDQFDQIWQSLPKVPDGRVEYREFLKTYSTRDDVIRSQSATPPRTQERKPSRESL--LSP----MMSPTKPY------SPDIPTPLPSASDVERKIKNTVDVLVQHHVILNNNELLSLWRKHDVNGKGRIVYKDFLRHFVLAMK 249 HSP 2 Score: 58.9214 bits (141), Expect = 7.343e-9 Identity = 59/315 (18.73%), Postives = 125/315 (39.68%), Query Frame = 3
Query: 3843 QIQEHINESIKVRPKLFLNSFKSKDYASLNFISKSDFREIIERFLFTVPDSHFEKFWSNLKKNEFDNVNYGEFVKMXXXXXXXXXXXXXXXXXXXXXXXXXXNNIETDEMDTDESIRKNLERLLNRNWQSIFNKCKAIDQAINECQTAEGSIPIDDFVYILSEYDDSLSAENLAAI-NLYKTCKNGRFIKYGDLIRSIVLSSRGSLISNENVTEVQKKFLNVGIKTTNSDNSKDYFPQLFKLRRQILENWKDLRKNFLQSDKKQNGAVSGNIFMTIMKTVCGQLSADLFYHLMKIYDQDGTGNFHYKRFIEDFLK 4784
++++ + E + R +F +D + +S R +++ +F + D F++ W +L K V Y EF+K S + ++ + + + E LL+ I + E I + V +L ++ L+ L ++ + GR + Y D +R VL+ + ++ ++ K + ++ S + + +R ++ E+WK++R+ F DK +G + F ++K L+ + F+H+ YD+D TG Y F+ L+
Sbjct: 69 EVKQRVKEVVMARFYRLAKAFTDQDSSRSGTVSPRGLRRVLDEHVFRMNDDQFDQIWQSLPKVPDGRVEYREFLKTYSTRDDVIRS-------------------QSATPPRTQERKPSRESLLSPMMSPTKPYSPDIPTPLPSASDVERKI--KNTVDVLVQHHVILNNNELLSLWRKHDVNGKGRIV-YKDFLRHFVLA----MKPQDDSKLIRPKLQPTKVPSSPGVRSDELIDVMATIRGKVQEDWKEMRRAFRAIDKDGSGLCNPLDFRQMLKRFKIDLNEEEFFHVYSFYDKDMTGKISYNEFLRAHLE 357 HSP 3 Score: 57.3806 bits (137), Expect = 2.810e-8 Identity = 64/309 (20.71%), Postives = 133/309 (43.04%), Query Frame = 3
Query: 2070 MSPEELIVRIKDRIVQNRLNYHELYKKYECND--KKGGINKSQLRKMLTQFGYTLTNEQFSKISEILNFPTSSKSRIDFNGFLAIFADNRESSNDLMTDVCFLPKSNHHVNDIRGDDNRNMNCQEVEDLLRQKIVNNFPDLMIAFHKFDRSNKGYLVKSDFQNFLLGNLIMIENDQFDLLCEKLGVGEKSKLTYNDFLNRFQIRDDMVDGHKWLDSKHRFND-PSTNAVPDMSAEDVYMLLPEKIYQYFHEISPTFIALVTSGTGFIRFRNLQHLLSRLQISMSKSEMKKLWKRYDTNNRNKLDYHDFL 2987
MS +E+ R+K+ +V R ++ L K + D + G ++ LR++L + + + ++QF +I + L P R+++ FL ++ + +D++ P R M + + P + + +R K + + L+ + +++ N++ L K V K ++ Y DFL F + D K + K + PS+ V DV + K+ + + E+ F A+ G+G + + +L R +I +++ E ++ YD + K+ Y++FL
Sbjct: 65 MSEDEVKQRVKE-VVMAR--FYRLAKAFTDQDSSRSGTVSPRGLRRVLDEHVFRMNDDQFDQIWQSL--PKVPDGRVEYREFLKTYS----TRDDVIRSQSATPPRTQERKPSRESLLSPMMSPT------KPYSPDIPTPLPSASDVERKIKNTV------DVLVQHHVILNNNELLSLWRKHDVNGKGRIVYKDFLRHFVLAMKPQDDSKLIRPKLQPTKVPSSPGVRSDELIDVMATIRGKVQEDWKEMRRAFRAIDKDGSGLCNPLDFRQMLKRFKIDLNEEEFFHVYSFYDKDMTGKISYNEFL 352
BLAST of EF-hand calcium-binding domain-containing protein 6 vs. Ensembl Nematostella
Match: EDO28943 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T520]) HSP 1 Score: 56.9954 bits (136), Expect = 7.168e-9 Identity = 41/177 (23.16%), Postives = 82/177 (46.33%), Query Frame = 3
Query: 2460 KIVNNFPDLMIAFHKFDRSNKGYLVKSDFQNFLLGNLIMIENDQFDLLCEKLGVGEKSKLTYNDFLNRFQIRDDMVDGHKWLDSKHRFND-PSTNAVPDMSAEDVYMLLPEKIYQYFHEISPTFIALVTSGTGFIRFRNLQHLLSRLQISMSKSEMKKLWKRYDTNNRNKLDYHDFL 2987
++ N+ L +F D N G++ ++ L+ + +++ N++ L K V K ++ Y DFL F + D K + K + PS+ V DV + K+ + + E+ F A+ G+G + + +L R +I +++ E ++ YD + K+ Y++FL
Sbjct: 24 EVCRNWQQLQKSFRNLDPDNTGHVSFHQLKDVLVQHYVILNNNELLSLWRKHDVDGKGRIVYKDFLRHFVLAMKPQDDSKLIRPKLQPTKVPSSPGVRSDELIDVMATIRGKVQEDWKEMRRAFRAIDKDGSGLCNPVDFRQMLKRFKIDLNEEEFFHVYSFYDKDMTGKISYNEFL 200 HSP 2 Score: 51.2174 bits (121), Expect = 7.666e-7 Identity = 31/130 (23.85%), Postives = 62/130 (47.69%), Query Frame = 3
Query: 4395 GRFIKYGDLIRSIVLSSRGSLISNENVTEVQKKFLNVGIKTTNSDNSKDYFPQLFKLRRQILENWKDLRKNFLQSDKKQNGAVSGNIFMTIMKTVCGQLSADLFYHLMKIYDQDGTGNFHYKRFIEDFLK 4784
GR + Y D +R VL+ + ++ ++ K + ++ S + + +R ++ E+WK++R+ F DK +G + F ++K L+ + F+H+ YD+D TG Y F+ L+
Sbjct: 81 GRIV-YKDFLRHFVLAMK----PQDDSKLIRPKLQPTKVPSSPGVRSDELIDVMATIRGKVQEDWKEMRRAFRAIDKDGSGLCNPVDFRQMLKRFKIDLNEEEFFHVYSFYDKDMTGKISYNEFLRAHLE 205 HSP 3 Score: 50.447 bits (119), Expect = 1.431e-6 Identity = 42/171 (24.56%), Postives = 75/171 (43.86%), Query Frame = 3
Query: 1086 LRKTFLVMDPNAEGFVTLEILYKILNQFAFPMSLNVFNQIMDKCAFKATGKIIWLSFLQKFQDPQSKHLNGSTIAIK-SDHKCNPIQGVNSKPNIDDLLELLSNHISNNYSSFRDAFLQINQTHKGKIPKSEFLKILNRFQFYISADQLSIILKFLDPHNEGYISYHHFLQ 1595
L+K+F +DP+ G V+ L +L Q ++ N + K G+I++ FL+ F + I K K GV S ID ++ + + ++ R AF I++ G +F ++L RF+ ++ ++ + F D G ISY+ FL+
Sbjct: 32 LQKSFRNLDPDNTGHVSFHQLKDVLVQHYVILNNNELLSLWRKHDVDGKGRIVYKDFLRHFVLAMKPQDDSKLIRPKLQPTKVPSSPGVRSDELID-VMATIRGKVQEDWKEMRRAFRAIDKDGSGLCNPVDFRQMLKRFKIDLNEEEFFHVYSFYDKDMTGKISYNEFLR 201
BLAST of EF-hand calcium-binding domain-containing protein 6 vs. Planmine SMEST
Match: SMESG000052556.1 (SMESG000052556.1) HSP 1 Score: 2090.85 bits (5416), Expect = 0.000e+0 Identity = 1060/1071 (98.97%), Postives = 1065/1071 (99.44%), Query Frame = 3
Query: 1305 IAIKSDHKCNPIQGVNSKPNIDDLLELLSNHISNNYSSFRDAFLQINQTHKGKIPKSEFLKILNRFQFYISADQLSIILKFLDPHNEGYISYHHFLQLFEQKENLSAHKWLNSNHKVVEPRKDLQLSSETIKSTISDNIGNKWSNVVHEFNLVDLNRTGYISVEDLKKIFKKLFGYLKDDDIDRLVAPLTLKDKKGRINYLELFNLLNVDTSPSDLEGLSTQIAESNDAQLKERHENHIEKMESIEKLGEARLNLMSPEELIVRIKDRIVQNRLNYHELYKKYECNDKKGGINKSQLRKMLTQFGYTLTNEQFSKISEILNFPTSSKSRIDFNGFLAIFADNRESSNDLMTDVCFLPKSNHHVNDIRGDDNRNMNCQEVEDLLRQKIVNNFPDLMIAFHKFDRSNKGYLVKSDFQNFLLGNLIMIENDQFDLLCEKLGVGEKSKLTYNDFLNRFQIRDDMVDGHKWLDSKHRFNDPSTNAVPDMSAEDVYMLLPEKIYQYFHEISPTFIALVTSGTGFIRFRNLQHLLSRLQISMSKSEMKKLWKRYDTNNRNKLDYHDFLYNLGLLEKFGCGDLMGPSAAIIQNSHNALVDHLVNQETKHQQSVDNQAVLGTHLHVEHLFEMVKRHLRVAYSDSALAFKAVANKKTLSGFFTINDLNKLLSQLHMFVTNDKFVQLAARMGFEGPDDSLSYEKFCCIFQDSLNIDGQEKTVGSIRINQDLENPSKIGIEEAGNLNSTQIASIIGKRIVDQYSEIEKTFLVMDPENSGYVSMNEFRNLLVGFCLSLTDKQFNKYRNSLSTYIVKSANTDDKIDYRYFMKGFNQKLNNLDIQXXXXXXXXXXXXXTYQQIQEHINESIKVRPKLFLNSFKSKDYASLNFISKSDFREIIERFLFTVPDSHFEKFWSNLKKNEFDNVNYGEFVKMXXXXXXXXXXXXXXXXXXXXXXXXXXNNIETDEMDTDESIRKNLERLLNRNWQSIFNKCKAIDQAINECQTAEGSIPIDDFVYILSEYDDSLSAENLAAINLYKTCKNGRFIKYGDLIRSIVLSSRGSLISNENVTEVQKKFLNVGIKT 4517
I++ D CNPIQGVNSKPNIDDLLELLSNHISNNYSSFRDAFLQINQTHKGKIPKSEFLKILNRFQFYISADQLSIILKFLDPHNEGYISYHHFLQLFEQKENLSAHKWLNSNHKVVEPRKDLQLSSETIKSTISDNIGNKWSNVVHEFNLVDLNRTGYISVEDLKKIFKKLFGYLKDDDIDRLVAPLTLKDKKGRINYLELFNLLNVDTSPSDLEGLSTQIAESNDAQLKERHENHIEKME I+KLGEARLNLMSPEELIVRIKDRIVQNRLNYHELYKKYECNDKKGGINKSQLRKMLTQFGYTLTNEQFSKISEILNFPTSSKSRIDFNGFLAIFADNRESSNDLMTDVCFLPKSNHHVNDIRGDDNRNMNCQEVEDLLRQKIVNNFPDLMIAFHKFDRSNKGYLVKSDFQNFLLGNLIMIENDQFDLLCEKLGVGEKSKLTYNDFLNRFQIRDDMVDGHKWLDSKHRFNDPSTNAVPDMSAEDVYMLLPEKIYQYFHEISPTFIALVTSGTGFIRFRNLQHLLSRLQISMSKSEMKKLWKRYDTNNRNKLDYHDFLYNLGLLEKFGCGDLMGPSAAIIQNSHNALVDHLVNQETKHQQSVDNQAVLGTHLHVEHLFEMVKRHLRVAYSDSALAFKAVANKKTL+GFFTINDLNKLLSQLHMFVTNDKFVQLAARMGFEGPDDSLSYEKFCCIFQDSLNIDGQEKTVGSIRINQDLENPSKIGIEEAGNLNSTQIASIIGKRIVDQYSEIEKTFLVMDPENSGYVSMNEFRNLLVGFCLSLTDKQFNKYRNSLSTYIVKSANTDDKIDYRYFMKGFNQK+NNLDIQSMSANPAANASNMTYQQIQEHINESIKVRPKLFLNSFKSKDYASLNFISKSDFREIIERFLFTVPDSHFEKFWSNLKKNEFDNVNYGEFVKMISESSETDDDKKSKISKTKVKIKEETNNIETDEMDTDESIRKNLERLLNRNWQSIFNKCKAIDQAINECQTAEGSIPIDDFVYILSEYDDSLSAENLAAINLYKTCKNGRFIKYGDLIRSIVLSSRGSLISNENVTEVQKKFLNVGIK
Sbjct: 28 ISLVIDLACNPIQGVNSKPNIDDLLELLSNHISNNYSSFRDAFLQINQTHKGKIPKSEFLKILNRFQFYISADQLSIILKFLDPHNEGYISYHHFLQLFEQKENLSAHKWLNSNHKVVEPRKDLQLSSETIKSTISDNIGNKWSNVVHEFNLVDLNRTGYISVEDLKKIFKKLFGYLKDDDIDRLVAPLTLKDKKGRINYLELFNLLNVDTSPSDLEGLSTQIAESNDAQLKERHENHIEKMEYIDKLGEARLNLMSPEELIVRIKDRIVQNRLNYHELYKKYECNDKKGGINKSQLRKMLTQFGYTLTNEQFSKISEILNFPTSSKSRIDFNGFLAIFADNRESSNDLMTDVCFLPKSNHHVNDIRGDDNRNMNCQEVEDLLRQKIVNNFPDLMIAFHKFDRSNKGYLVKSDFQNFLLGNLIMIENDQFDLLCEKLGVGEKSKLTYNDFLNRFQIRDDMVDGHKWLDSKHRFNDPSTNAVPDMSAEDVYMLLPEKIYQYFHEISPTFIALVTSGTGFIRFRNLQHLLSRLQISMSKSEMKKLWKRYDTNNRNKLDYHDFLYNLGLLEKFGCGDLMGPSAAIIQNSHNALVDHLVNQETKHQQSVDNQAVLGTHLHVEHLFEMVKRHLRVAYSDSALAFKAVANKKTLTGFFTINDLNKLLSQLHMFVTNDKFVQLAARMGFEGPDDSLSYEKFCCIFQDSLNIDGQEKTVGSIRINQDLENPSKIGIEEAGNLNSTQIASIIGKRIVDQYSEIEKTFLVMDPENSGYVSMNEFRNLLVGFCLSLTDKQFNKYRNSLSTYIVKSANTDDKIDYRYFMKGFNQKVNNLDIQSMSANPAANASNMTYQQIQEHINESIKVRPKLFLNSFKSKDYASLNFISKSDFREIIERFLFTVPDSHFEKFWSNLKKNEFDNVNYGEFVKMISESSETDDDKKSKISKTKVKIKEETNNIETDEMDTDESIRKNLERLLNRNWQSIFNKCKAIDQAINECQTAEGSIPIDDFVYILSEYDDSLSAENLAAINLYKTCKNGRFIKYGDLIRSIVLSSRGSLISNENVTEVQKKFLNVGIKV 1098 HSP 2 Score: 113.235 bits (282), Expect = 8.561e-25 Identity = 171/797 (21.46%), Postives = 348/797 (43.66%), Query Frame = 3
Query: 711 IMKAFKLFDFNSDYCIQRHRLKKVMENFCCPLTEQQFDKLVARFS-QSQNGNVNYMEFMKGMGI---PIDNQGTIQTKNSEETINIDTRNTKGSRIFEAALIENGLLGSNEL-----EQLLVIFRKKIED---QFSVLRKTFLVMDPNAEGFVTLEILYKILNQFAFPMSLNVFNQIMDKCAFKATGK--IIWLSFLQKFQDPQSKH---LNGSTIAIKSDHKCNPIQG-VNSKPNIDDLLELLSNHISNNYSSFRDAFLQINQTHKGKIPKSEFLKILNRFQFYISADQLSIILKFLDPHNEGYISYHHFLQLFEQKENL-SAHKWLNSNHKVVEPRKDL--QLSSETIKSTISDNIGNKWSNVVHEFNLVDLNRTGYISVEDLKKIFKKLFGYLKDDDIDRLVAPLTLKDKKGRINYLE-LFNLLNVDT-SPSDLEGLSTQIAESNDAQLKERHEN----HIEKMESIEKLGEARLNLMSPEELIVRIKDRIVQNRLNYHE--LYKKYECNDK--KGGINKSQLRKMLTQFGYTLTNEQFSKISEILNFPTSSKSRIDFNGFLAIFADNRE-SSNDLMTDVCFLPKSNHHVNDIRGDDNRNMNCQEVEDLLRQKIVNNFPDLMIAFHKFDRSNKGYLVKSDFQNFLLGNLIMIENDQFDLLCEKL------GVGEKSKLTYNDFLNRFQIRDDMVDGHKWLDSKHRFNDPSTNAVPDMSAEDVYMLLPEKIYQYFHEISPTFIALVTSGTGFIRFRNLQHLLSRLQISMSKSEMKKLWKRYDTNNRNKLDYHDFL 2987
++ F L D N I LKK+ + L + D+LVA + + + G +NY+E + + P D +G + T+ +E + + + I + I+ LG L E+L+V + +I + L K + D +G + L K+L QF + ++ F++I + F + K I + FL F D + + KS+H N I+G N N ++ +LL I NN+ AF + ++++KG + KS+F L I DQ ++ + L + ++Y+ FL F+ ++++ HKWL+S H+ +P + +S+E + + + I + + F + + TG+I +L+ + +L + ++ +L + + +++Y + L+NL ++ DL G S I +++ L + N H + +++ LG + E L +K + R+ Y + L K N K G + L K+L+Q +TN++F +++ + F S + + F IF D+ + + + + + I ++ N+N ++ ++ ++IV+ + ++ F D N GY+ ++F+N L+G + + + QF+ L K+ Y F+ F + + LD + +P+ NA +M+ + + + E I +F + + FI + + ++ R ++ S +K W N + ++Y +F+
Sbjct: 173 VVHEFNLVDLNRTGYISVEDLKKIFKKLFGYLKDDDIDRLVAPLTLKDKKGRINYLELFNLLNVDTSPSDLEG-LSTQIAESN-DAQLKERHENHIEKMEYIDK--LGEARLNLMSPEELIVRIKDRIVQNRLNYHELYKKYECND--KKGGINKSQLRKMLTQFGYTLTNEQFSKISEILNFPTSSKSRIDFNGFLAIFADNRESSNDLMTDVCFLPKSNHHVNDIRGDDNRNMNCQEVEDLLRQKIVNNFPDLMIAFHKFDRSNKGYLVKSDFQNFLLGNLIMIENDQFDLLCEKLGVGEKSKLTYNDFLNRFQIRDDMVDGHKWLDSKHRFNDPSTNAVPDMSAEDVYMLLPEKIYQYFHEISPTFIALVTSGTGFIRFRNLQHLLSRLQISMSKSEMKKLWKRYD-TNNRNKLDYHDFLYNLGLLEKFGCGDLMGPSAAIIQNSHNALVDHLVNQETKHQQSVDNQAVLGTH----LHVEHLFEMVKRHL---RVAYSDSALAFKAVANKKTLTGFFTINDLNKLLSQLHMFVTNDKFVQLAARMGFEGPDDS-LSYEKFCCIFQDSLNIDGQEKTVGSIRINQDLENPSKIGIEEAGNLNSTQIASIIGKRIVDQYSEIEKTFLVMDPENSGYVSMNEFRNLLVGFCLSLTDKQFNKYRNSLSTYIVKSANTDDKIDYRYFMKGFNQKVNN------LDIQSMSANPAANA-SNMTYQQIQEHINESIKVRPKLFLNSFKSKDYASLNFISKSDFREIIERFLFTVPDSHFEKFWSNLKKNEFDNVNYGEFV 947 HSP 3 Score: 77.0258 bits (188), Expect = 1.069e-13 Identity = 211/980 (21.53%), Postives = 393/980 (40.10%), Query Frame = 3
Query: 1011 GLLGSNELEQLLVIFRKKIEDQFSVLRKTFLVMDPNAEGFVTLEILYKILNQFAFPMSLNVFNQIMDKCAFKATGKIIWLSFLQKFQDPQSKHLNGSTIAIKSDHK-CNPIQGVNSKPNIDDLLELLSNHISNNYSSFRDAFLQINQTHKGKIPKSEFLKILNRFQFYISADQLSIILKFLDPHN-EGYISYHHFLQLF------EQKENLSAH---------KWLNSNH--------KVVEPRKDLQLSSETIKSTISDNIGNKWSNVVHEFNLVDLN-RTGYISVEDLKKIFKKLFGY-LKDDDIDRLVAPLTL-KDKKGRIN---YLELFNLLNVDTSPSDLEGLSTQIAESNDAQLKERHENHIEKMESIEKLGEARLNLMSPEELIVRIKDRIVQNRLNYHELYKKYECNDKKGGINKSQLRKMLTQFGYTLTNEQFSKISEILNFPTSSKSRIDFNGFLAIFADNRESSNDLMTDVCFLPKSNHHVNDIRGDDNRNMNCQEVEDLLRQKIVNNFPDLMIAFHKFDRSNKGYLVKSDFQNFLLGNLIMIENDQFDLLCEKLGVGEKSKLTYNDF------LNRFQIRDDM-------VDGHKWL-------DSKHRFN-DPSTNAVPDMSAEDVYMLLPEKIYQYFHEISPTFIALVT--SGTGFIRFRNLQHLLSRLQISMSKSEMKKLWKRYD-TNNRNKLDYHDFLYNLGLLEKFGCGDLMGPSAAIIQNSHNALVDHLVNQETKHQQSVDNQAVLGT----HLHVEHLFEMVKRHLRVAYSDSALAFKAVANKKTLSGFFTINDLNKLLSQLHMFVTNDKFVQLAARMGF-----EGPDDSLSYEKFCCIFQDSLNIDGQEKTVGSIRINQDLENPSKIGIEEAGNLNSTQIASIIGKRIVDQYSEIEKTFLVMDPENSGYVSMNEFRNLLVGFCLSLTDKQFNKYRNSLSTYIVKSANTDDKIDYRYFMK 3758
G+ ++ LL + I + +S R FL ++ +G + KILN+F F +S + + I+ G I + FLQ F+ Q ++L+ + S+HK P + + + + + + +S++I N +S+ F ++ G I + KI + Y+ D + ++ L + +G I+Y L E LS K + NH K+ E R +L +S E + I D I N + + N + G I+ L+K+ + FGY L ++ ++ L K RI+ +L +F + S +DL + +SN H N I G+ N M+ +E+ ++ +IV N + + K++ ++ KG + KS + L + N+QF + E L KS++ +N FL NR D M D S H ND + +M+ ++V LL +KI F ++ F S G++ + Q+ L I + + L ++ ++KL Y+DF L +F D M + H L ++KH+ + D + E ++ ++ + + + + F A+ + TGF +L LLS+L + ++ + +L R + L Y EKF C I Q+S N + Q ++N + +G +L+ + ++ + + YS+ F + + SG+ ++N+ LL + +T+ +F + + DD + Y F F +N N D+++ S A N+ QI I + I + +F D + ++S ++FR ++ F ++ D F K+ ++L N D ++Y F+K
Sbjct: 41 GVNSKPNIDDLLELLSNHISNNYSSFRDAFLQINQTHKGKIPKSEFLKILNRFQFYISADQLSIILKFLDPHNEGYISYHHFLQLFE--QKENLSAHK-WLNSNHKVVEPRKDL--QLSSETIKSTISDNIGNKWSNVVHEFNLVDLNRTGYISVEDLKKIFKKLFGYLKDDDIDRLVAPLTLKDKKGRINYLELFNLLNVDTSPSDLEGLSTQIAESNDAQLKERHENHIEKMEYIDKLGEARLNL-MSPEELIVRIKDRIVQNRLNYHELYKKYECNDKKGGINKSQLRKMLTQ-FGYTLTNEQFSKISEILNFPTSSKSRIDFNGFLAIF--ADNRESSNDLMTDVCFLPKSN------HHVNDIR--------GDDNRN-MNCQEVEDLLRQKIVNNFPDLMIAFHKFDRSN-KGYLVKSDFQNFLLGNLIMIENDQFDLLCEKLG--VGEKSKLTYNDFL-----NRFQIRDDMVDGHKWLDSKHRFNDPSTNAVPDMSAEDVYMLLPEKIYQYFHEISPTFIALVTSGTGFIRFRNLQHLLSRLQISMSKSEMKKLWKRYDTNNRNKLDYHDFLYNLGLLEKFGCGDLMGPSAAIIQNSHNALVDHLVNQETKHQQSVDNQAVLGTHLHVEHLFEMVKRHLRVAYSDSALAFKAVANKKTLTGFFTINDLNKLLSQLHMFVTNDKFVQLAARMGFEGPDDSLSY----------EKFCC---------IFQDSLNIDGQEKTVGSIRINQDLENPSKIGIEEAGNLNSTQIASIIGKRIVDQYSEIEKTFLVMDPEN--SGYVSMNEFRNLLVGFCLSLTDKQFNKYRNSLSTYIVKSANTDDKIDYRYFMKGFNQKVN-------------NLDIQSMSANPAANASNMTYQQIQEHINESIKVRPKLFLNSFKSKDYASLNFISKSDFREIIERFLFTVPDSHFEKFWSNL------KKNEFDNVNYGEFVK 948
BLAST of EF-hand calcium-binding domain-containing protein 6 vs. Planmine SMEST
Match: SMESG000052556.1 (SMESG000052556.1) HSP 1 Score: 2088.54 bits (5410), Expect = 0.000e+0 Identity = 1060/1071 (98.97%), Postives = 1065/1071 (99.44%), Query Frame = 3
Query: 1305 IAIKSDHKCNPIQGVNSKPNIDDLLELLSNHISNNYSSFRDAFLQINQTHKGKIPKSEFLKILNRFQFYISADQLSIILKFLDPHNEGYISYHHFLQLFEQKENLSAHKWLNSNHKVVEPRKDLQLSSETIKSTISDNIGNKWSNVVHEFNLVDLNRTGYISVEDLKKIFKKLFGYLKDDDIDRLVAPLTLKDKKGRINYLELFNLLNVDTSPSDLEGLSTQIAESNDAQLKERHENHIEKMESIEKLGEARLNLMSPEELIVRIKDRIVQNRLNYHELYKKYECNDKKGGINKSQLRKMLTQFGYTLTNEQFSKISEILNFPTSSKSRIDFNGFLAIFADNRESSNDLMTDVCFLPKSNHHVNDIRGDDNRNMNCQEVEDLLRQKIVNNFPDLMIAFHKFDRSNKGYLVKSDFQNFLLGNLIMIENDQFDLLCEKLGVGEKSKLTYNDFLNRFQIRDDMVDGHKWLDSKHRFNDPSTNAVPDMSAEDVYMLLPEKIYQYFHEISPTFIALVTSGTGFIRFRNLQHLLSRLQISMSKSEMKKLWKRYDTNNRNKLDYHDFLYNLGLLEKFGCGDLMGPSAAIIQNSHNALVDHLVNQETKHQQSVDNQAVLGTHLHVEHLFEMVKRHLRVAYSDSALAFKAVANKKTLSGFFTINDLNKLLSQLHMFVTNDKFVQLAARMGFEGPDDSLSYEKFCCIFQDSLNIDGQEKTVGSIRINQDLENPSKIGIEEAGNLNSTQIASIIGKRIVDQYSEIEKTFLVMDPENSGYVSMNEFRNLLVGFCLSLTDKQFNKYRNSLSTYIVKSANTDDKIDYRYFMKGFNQKLNNLDIQXXXXXXXXXXXXXTYQQIQEHINESIKVRPKLFLNSFKSKDYASLNFISKSDFREIIERFLFTVPDSHFEKFWSNLKKNEFDNVNYGEFVKMXXXXXXXXXXXXXXXXXXXXXXXXXXNNIETDEMDTDESIRKNLERLLNRNWQSIFNKCKAIDQAINECQTAEGSIPIDDFVYILSEYDDSLSAENLAAINLYKTCKNGRFIKYGDLIRSIVLSSRGSLISNENVTEVQKKFLNVGIKT 4517
I++ D CNPIQGVNSKPNIDDLLELLSNHISNNYSSFRDAFLQINQTHKGKIPKSEFLKILNRFQFYISADQLSIILKFLDPHNEGYISYHHFLQLFEQKENLSAHKWLNSNHKVVEPRKDLQLSSETIKSTISDNIGNKWSNVVHEFNLVDLNRTGYISVEDLKKIFKKLFGYLKDDDIDRLVAPLTLKDKKGRINYLELFNLLNVDTSPSDLEGLSTQIAESNDAQLKERHENHIEKME I+KLGEARLNLMSPEELIVRIKDRIVQNRLNYHELYKKYECNDKKGGINKSQLRKMLTQFGYTLTNEQFSKISEILNFPTSSKSRIDFNGFLAIFADNRESSNDLMTDVCFLPKSNHHVNDIRGDDNRNMNCQEVEDLLRQKIVNNFPDLMIAFHKFDRSNKGYLVKSDFQNFLLGNLIMIENDQFDLLCEKLGVGEKSKLTYNDFLNRFQIRDDMVDGHKWLDSKHRFNDPSTNAVPDMSAEDVYMLLPEKIYQYFHEISPTFIALVTSGTGFIRFRNLQHLLSRLQISMSKSEMKKLWKRYDTNNRNKLDYHDFLYNLGLLEKFGCGDLMGPSAAIIQNSHNALVDHLVNQETKHQQSVDNQAVLGTHLHVEHLFEMVKRHLRVAYSDSALAFKAVANKKTL+GFFTINDLNKLLSQLHMFVTNDKFVQLAARMGFEGPDDSLSYEKFCCIFQDSLNIDGQEKTVGSIRINQDLENPSKIGIEEAGNLNSTQIASIIGKRIVDQYSEIEKTFLVMDPENSGYVSMNEFRNLLVGFCLSLTDKQFNKYRNSLSTYIVKSANTDDKIDYRYFMKGFNQK+NNLDIQSMSANPAANASNMTYQQIQEHINESIKVRPKLFLNSFKSKDYASLNFISKSDFREIIERFLFTVPDSHFEKFWSNLKKNEFDNVNYGEFVKMISESSETDDDKKSKISKTKVKIKEETNNIETDEMDTDESIRKNLERLLNRNWQSIFNKCKAIDQAINECQTAEGSIPIDDFVYILSEYDDSLSAENLAAINLYKTCKNGRFIKYGDLIRSIVLSSRGSLISNENVTEVQKKFLNVGIK
Sbjct: 10 ISLVIDLACNPIQGVNSKPNIDDLLELLSNHISNNYSSFRDAFLQINQTHKGKIPKSEFLKILNRFQFYISADQLSIILKFLDPHNEGYISYHHFLQLFEQKENLSAHKWLNSNHKVVEPRKDLQLSSETIKSTISDNIGNKWSNVVHEFNLVDLNRTGYISVEDLKKIFKKLFGYLKDDDIDRLVAPLTLKDKKGRINYLELFNLLNVDTSPSDLEGLSTQIAESNDAQLKERHENHIEKMEYIDKLGEARLNLMSPEELIVRIKDRIVQNRLNYHELYKKYECNDKKGGINKSQLRKMLTQFGYTLTNEQFSKISEILNFPTSSKSRIDFNGFLAIFADNRESSNDLMTDVCFLPKSNHHVNDIRGDDNRNMNCQEVEDLLRQKIVNNFPDLMIAFHKFDRSNKGYLVKSDFQNFLLGNLIMIENDQFDLLCEKLGVGEKSKLTYNDFLNRFQIRDDMVDGHKWLDSKHRFNDPSTNAVPDMSAEDVYMLLPEKIYQYFHEISPTFIALVTSGTGFIRFRNLQHLLSRLQISMSKSEMKKLWKRYDTNNRNKLDYHDFLYNLGLLEKFGCGDLMGPSAAIIQNSHNALVDHLVNQETKHQQSVDNQAVLGTHLHVEHLFEMVKRHLRVAYSDSALAFKAVANKKTLTGFFTINDLNKLLSQLHMFVTNDKFVQLAARMGFEGPDDSLSYEKFCCIFQDSLNIDGQEKTVGSIRINQDLENPSKIGIEEAGNLNSTQIASIIGKRIVDQYSEIEKTFLVMDPENSGYVSMNEFRNLLVGFCLSLTDKQFNKYRNSLSTYIVKSANTDDKIDYRYFMKGFNQKVNNLDIQSMSANPAANASNMTYQQIQEHINESIKVRPKLFLNSFKSKDYASLNFISKSDFREIIERFLFTVPDSHFEKFWSNLKKNEFDNVNYGEFVKMISESSETDDDKKSKISKTKVKIKEETNNIETDEMDTDESIRKNLERLLNRNWQSIFNKCKAIDQAINECQTAEGSIPIDDFVYILSEYDDSLSAENLAAINLYKTCKNGRFIKYGDLIRSIVLSSRGSLISNENVTEVQKKFLNVGIKV 1080 HSP 2 Score: 113.235 bits (282), Expect = 8.093e-25 Identity = 170/797 (21.33%), Postives = 346/797 (43.41%), Query Frame = 3
Query: 711 IMKAFKLFDFNSDYCIQRHRLKKVMENFCCPLTEQQFDKLVARFS-QSQNGNVNYMEFMKGMGI---PIDNQGTIQTKNSEETINIDTRNTKGSRIFEAALIENGLLGSNEL-----EQLLVIFRKKIED---QFSVLRKTFLVMDPNAEGFVTLEILYKILNQFAFPMSLNVFNQIMDKCAFKATGK--IIWLSFLQKFQDPQSKH---LNGSTIAIKSDHKCNPIQG-VNSKPNIDDLLELLSNHISNNYSSFRDAFLQINQTHKGKIPKSEFLKILNRFQFYISADQLSIILKFLDPHNEGYISYHHFLQLFEQKENL-SAHKWLNSNHKVVEPRKDL--QLSSETIKSTISDNIGNKWSNVVHEFNLVDLNRTGYISVEDLKKIFKKLFGYLKDDDIDRLVAPLTLKDKKGRINYLE-LFNLLNVDT-SPSDLEGLSTQIAESNDAQLKERHEN----HIEKMESIEKLGEARLNLMSPEELIVRIKDRIVQNRLNYHE--LYKKYECNDK--KGGINKSQLRKMLTQFGYTLTNEQFSKISEILNFPTSSKSRIDFNGFLAIFADNRE-SSNDLMTDVCFLPKSNHHVNDIRGDDNRNMNCQEVEDLLRQKIVNNFPDLMIAFHKFDRSNKGYLVKSDFQNFLLGNLIMIENDQFDLLCEKL------GVGEKSKLTYNDFLNRFQIRDDMVDGHKWLDSKHRFNDPSTNAVPDMSAEDVYMLLPEKIYQYFHEISPTFIALVTSGTGFIRFRNLQHLLSRLQISMSKSEMKKLWKRYDTNNRNKLDYHDFL 2987
++ F L D N I LKK+ + L + D+LVA + + + G +NY+E + + P D +G + E+ + + + I + I+ LG L E+L+V + +I + L K + D +G + L K+L QF + ++ F++I + F + K I + FL F D + + KS+H N I+G N N ++ +LL I NN+ AF + ++++KG + KS+F L I DQ ++ + L + ++Y+ FL F+ ++++ HKWL+S H+ +P + +S+E + + + I + + F + + TG+I +L+ + +L + ++ +L + + +++Y + L+NL ++ DL G S I +++ L + N H + +++ LG + E L +K + R+ Y + L K N K G + L K+L+Q +TN++F +++ + F S + + F IF D+ + + + + + I ++ N+N ++ ++ ++IV+ + ++ F D N GY+ ++F+N L+G + + + QF+ L K+ Y F+ F + + LD + +P+ NA +M+ + + + E I +F + + FI + + ++ R ++ S +K W N + ++Y +F+
Sbjct: 155 VVHEFNLVDLNRTGYISVEDLKKIFKKLFGYLKDDDIDRLVAPLTLKDKKGRINYLELFNLLNVDTSPSDLEGL--STQIAESNDAQLKERHENHIEKMEYIDK--LGEARLNLMSPEELIVRIKDRIVQNRLNYHELYKKYECND--KKGGINKSQLRKMLTQFGYTLTNEQFSKISEILNFPTSSKSRIDFNGFLAIFADNRESSNDLMTDVCFLPKSNHHVNDIRGDDNRNMNCQEVEDLLRQKIVNNFPDLMIAFHKFDRSNKGYLVKSDFQNFLLGNLIMIENDQFDLLCEKLGVGEKSKLTYNDFLNRFQIRDDMVDGHKWLDSKHRFNDPSTNAVPDMSAEDVYMLLPEKIYQYFHEISPTFIALVTSGTGFIRFRNLQHLLSRLQISMSKSEMKKLWKRYD-TNNRNKLDYHDFLYNLGLLEKFGCGDLMGPSAAIIQNSHNALVDHLVNQETKHQQSVDNQAVLGTH----LHVEHLFEMVKRHL---RVAYSDSALAFKAVANKKTLTGFFTINDLNKLLSQLHMFVTNDKFVQLAARMGFEGPDDS-LSYEKFCCIFQDSLNIDGQEKTVGSIRINQDLENPSKIGIEEAGNLNSTQIASIIGKRIVDQYSEIEKTFLVMDPENSGYVSMNEFRNLLVGFCLSLTDKQFNKYRNSLSTYIVKSANTDDKIDYRYFMKGFNQKVNN------LDIQSMSANPAANA-SNMTYQQIQEHINESIKVRPKLFLNSFKSKDYASLNFISKSDFREIIERFLFTVPDSHFEKFWSNLKKNEFDNVNYGEFV 929 HSP 3 Score: 77.0258 bits (188), Expect = 1.126e-13 Identity = 211/980 (21.53%), Postives = 393/980 (40.10%), Query Frame = 3
Query: 1011 GLLGSNELEQLLVIFRKKIEDQFSVLRKTFLVMDPNAEGFVTLEILYKILNQFAFPMSLNVFNQIMDKCAFKATGKIIWLSFLQKFQDPQSKHLNGSTIAIKSDHK-CNPIQGVNSKPNIDDLLELLSNHISNNYSSFRDAFLQINQTHKGKIPKSEFLKILNRFQFYISADQLSIILKFLDPHN-EGYISYHHFLQLF------EQKENLSAH---------KWLNSNH--------KVVEPRKDLQLSSETIKSTISDNIGNKWSNVVHEFNLVDLN-RTGYISVEDLKKIFKKLFGY-LKDDDIDRLVAPLTL-KDKKGRIN---YLELFNLLNVDTSPSDLEGLSTQIAESNDAQLKERHENHIEKMESIEKLGEARLNLMSPEELIVRIKDRIVQNRLNYHELYKKYECNDKKGGINKSQLRKMLTQFGYTLTNEQFSKISEILNFPTSSKSRIDFNGFLAIFADNRESSNDLMTDVCFLPKSNHHVNDIRGDDNRNMNCQEVEDLLRQKIVNNFPDLMIAFHKFDRSNKGYLVKSDFQNFLLGNLIMIENDQFDLLCEKLGVGEKSKLTYNDF------LNRFQIRDDM-------VDGHKWL-------DSKHRFN-DPSTNAVPDMSAEDVYMLLPEKIYQYFHEISPTFIALVT--SGTGFIRFRNLQHLLSRLQISMSKSEMKKLWKRYD-TNNRNKLDYHDFLYNLGLLEKFGCGDLMGPSAAIIQNSHNALVDHLVNQETKHQQSVDNQAVLGT----HLHVEHLFEMVKRHLRVAYSDSALAFKAVANKKTLSGFFTINDLNKLLSQLHMFVTNDKFVQLAARMGF-----EGPDDSLSYEKFCCIFQDSLNIDGQEKTVGSIRINQDLENPSKIGIEEAGNLNSTQIASIIGKRIVDQYSEIEKTFLVMDPENSGYVSMNEFRNLLVGFCLSLTDKQFNKYRNSLSTYIVKSANTDDKIDYRYFMK 3758
G+ ++ LL + I + +S R FL ++ +G + KILN+F F +S + + I+ G I + FLQ F+ Q ++L+ + S+HK P + + + + + + +S++I N +S+ F ++ G I + KI + Y+ D + ++ L + +G I+Y L E LS K + NH K+ E R +L +S E + I D I N + + N + G I+ L+K+ + FGY L ++ ++ L K RI+ +L +F + S +DL + +SN H N I G+ N M+ +E+ ++ +IV N + + K++ ++ KG + KS + L + N+QF + E L KS++ +N FL NR D M D S H ND + +M+ ++V LL +KI F ++ F S G++ + Q+ L I + + L ++ ++KL Y+DF L +F D M + H L ++KH+ + D + E ++ ++ + + + + F A+ + TGF +L LLS+L + ++ + +L R + L Y EKF C I Q+S N + Q ++N + +G +L+ + ++ + + YS+ F + + SG+ ++N+ LL + +T+ +F + + DD + Y F F +N N D+++ S A N+ QI I + I + +F D + ++S ++FR ++ F ++ D F K+ ++L N D ++Y F+K
Sbjct: 23 GVNSKPNIDDLLELLSNHISNNYSSFRDAFLQINQTHKGKIPKSEFLKILNRFQFYISADQLSIILKFLDPHNEGYISYHHFLQLFE--QKENLSAHK-WLNSNHKVVEPRKDL--QLSSETIKSTISDNIGNKWSNVVHEFNLVDLNRTGYISVEDLKKIFKKLFGYLKDDDIDRLVAPLTLKDKKGRINYLELFNLLNVDTSPSDLEGLSTQIAESNDAQLKERHENHIEKMEYIDKLGEARLNL-MSPEELIVRIKDRIVQNRLNYHELYKKYECNDKKGGINKSQLRKMLTQ-FGYTLTNEQFSKISEILNFPTSSKSRIDFNGFLAIF--ADNRESSNDLMTDVCFLPKSN------HHVNDIR--------GDDNRN-MNCQEVEDLLRQKIVNNFPDLMIAFHKFDRSN-KGYLVKSDFQNFLLGNLIMIENDQFDLLCEKLG--VGEKSKLTYNDFL-----NRFQIRDDMVDGHKWLDSKHRFNDPSTNAVPDMSAEDVYMLLPEKIYQYFHEISPTFIALVTSGTGFIRFRNLQHLLSRLQISMSKSEMKKLWKRYDTNNRNKLDYHDFLYNLGLLEKFGCGDLMGPSAAIIQNSHNALVDHLVNQETKHQQSVDNQAVLGTHLHVEHLFEMVKRHLRVAYSDSALAFKAVANKKTLTGFFTINDLNKLLSQLHMFVTNDKFVQLAARMGFEGPDDSLSY----------EKFCC---------IFQDSLNIDGQEKTVGSIRINQDLENPSKIGIEEAGNLNSTQIASIIGKRIVDQYSEIEKTFLVMDPEN--SGYVSMNEFRNLLVGFCLSLTDKQFNKYRNSLSTYIVKSANTDDKIDYRYFMKGFNQKVN-------------NLDIQSMSANPAANASNMTYQQIQEHINESIKVRPKLFLNSFKSKDYASLNFISKSDFREIIERFLFTVPDSHFEKFWSNL------KKNEFDNVNYGEFVK 930
BLAST of EF-hand calcium-binding domain-containing protein 6 vs. Planmine SMEST
Match: SMESG000052552.1 (SMESG000052552.1) HSP 1 Score: 829.706 bits (2142), Expect = 0.000e+0 Identity = 419/424 (98.82%), Postives = 422/424 (99.53%), Query Frame = 3
Query: 57 MTTDITDSLIVRKSIYREPIQSNSLNQPEKNSTIKDANYKMDPKLQISEINPIVKSQLLKNHKEILKALQTFDIECSGLVTKGELRRVLDSYCLPMTTDQYEQFLSHNSINYSKGSINYKELIDKIYDKTSAVGHFKKNIKLPQLQRSATTICLQNKHFTLPTTRRSSGKISRSQLFXXXXXXXXXXXXXXXXWNYNAYTIDDIIPILQMQISRNPNGIMKAFKLFDFNSDYCIQRHRLKKVMENFCCPLTEQQFDKLVARFSQSQNGNVNYMEFMKGMGIPIDNQGTIQTKNSEETINIDTRNTKGSRIFEAALIENGLLGSNELEQLLVIFRKKIEDQFSVLRKTFLVMDPNAEGFVTLEILYKILNQFAFPMSLNVFNQIMDKCAFKATGKIIWLSFLQKFQDPQSKHLNGSTIAIKSDHK 1328
MTTDITDSLIVRKSI REPIQSNSLNQPEKNSTIKDANYKM+PKLQISEINPIVKSQLLKNHKEILKALQTFDIECSGLVTKGELRRVLDSYCLPMTTDQYEQFLSHNSINYSKGSINYKELIDKIYDKTSAVGHF+KNIKLPQLQRSATTICLQNKHFTLPTTRRSSGKISRSQLFSQCSSRSLNSQQSSRSWNYNAYTIDDIIPILQMQISRNPNGIMKAFKLFDFNSDYCIQRHRLKKVMENFCCPLTEQQFDKLVARFSQSQNGNVNYMEFMKGMGIPIDNQGTIQTKNSEETINIDTRNTK SRIFEAALIENGLLGSNELEQLLVIFRKKIEDQFSVLRKTFLVMDPNAEGFVTLEILYKILNQFAFPMSLNVFNQIMDKCAFKATGKI+WLSFLQKFQDPQSKHLNGSTIAIKSDHK
Sbjct: 1 MTTDITDSLIVRKSICREPIQSNSLNQPEKNSTIKDANYKMNPKLQISEINPIVKSQLLKNHKEILKALQTFDIECSGLVTKGELRRVLDSYCLPMTTDQYEQFLSHNSINYSKGSINYKELIDKIYDKTSAVGHFEKNIKLPQLQRSATTICLQNKHFTLPTTRRSSGKISRSQLFSQCSSRSLNSQQSSRSWNYNAYTIDDIIPILQMQISRNPNGIMKAFKLFDFNSDYCIQRHRLKKVMENFCCPLTEQQFDKLVARFSQSQNGNVNYMEFMKGMGIPIDNQGTIQTKNSEETINIDTRNTKESRIFEAALIENGLLGSNELEQLLVIFRKKIEDQFSVLRKTFLVMDPNAEGFVTLEILYKILNQFAFPMSLNVFNQIMDKCAFKATGKIVWLSFLQKFQDPQSKHLNGSTIAIKSDHK 424 HSP 2 Score: 72.0182 bits (175), Expect = 1.533e-12 Identity = 83/381 (21.78%), Postives = 168/381 (44.09%), Query Frame = 3
Query: 657 IDDIIPILQMQISRNPNGIMKAFKLFDFNSDYCIQRHRLKKVMENFCCPLTEQQFDKLVARFS-QSQNGNVNYME--------------FMKGMGIPI--DNQGTIQTKNSEETINIDTRNT-KGSRIFEAALIENGLLGSNE-----------LEQLLVIFRKKIEDQFSVLRKTFLVMDPNAEGFVTLEILYKILNQFAFPMSLNVFNQIMDKCAFKATGKIIWLSFLQKFQDPQSKHLNGSTIAIKSDHKCNPIQGVNSKPN-----------------IDDLLELLSNHISNNYSSFRDAFLQINQTHKGKIPKSEFLKILNRFQFYISADQLSIILKFLDPHNEGYISYHHFLQLFE--QKENLSAHK-WLNSNHK 1652
I +I PI++ Q+ +N I+KA + FD + + L++V++++C P+T Q+++ ++ S G++NY E F K + +P + TI +N T+ R++ K SR + + L S + ++ ++ I + +I + + K F + D N++ + L K++ F P++ F++++ + + G + ++ F++ P N TI K+ + I N+K + ++ LL + I + +S R FL ++ +G + KILN+F F +S + + I+ G I + FLQ F+ Q ++L+ + S+HK
Sbjct: 47 ISEINPIVKSQLLKNHKEILKALQTFDIECSGLVTKGELRRVLDSYCLPMTTDQYEQFLSHNSINYSKGSINYKELIDKIYDKTSAVGHFEKNIKLPQLQRSATTICLQNKHFTLPTTRRSSGKISRSQLFSQCSSRSLNSQQSSRSWNYNAYTIDDIIPILQMQISRNPNGIMKAFKLFDFNSDYCIQRHRLKKVMENFCCPLTEQQFDKLVARFSQSQNGNVNYMEFMKGMGIPID---NQGTIQTKNSEETINIDTRNTKESRIFEAALIENGLLGSNELEQLLVIFRKKIEDQFSVLRKTFLVMDPNAEGFVTLEILYKILNQFAFPMSLNVFNQIMDKCAFKATGKIVWLSFLQKFQDPQSKHLNGSTIAIKSDHK 424 The following BLAST results are available for this feature:
BLAST of EF-hand calcium-binding domain-containing protein 6 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 3
BLAST of EF-hand calcium-binding domain-containing protein 6 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of EF-hand calcium-binding domain-containing protein 6 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of EF-hand calcium-binding domain-containing protein 6 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of EF-hand calcium-binding domain-containing protein 6 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 3
BLAST of EF-hand calcium-binding domain-containing protein 6 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 1
BLAST of EF-hand calcium-binding domain-containing protein 6 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 4
BLAST of EF-hand calcium-binding domain-containing protein 6 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of EF-hand calcium-binding domain-containing protein 6 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 2
BLAST of EF-hand calcium-binding domain-containing protein 6 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 3
BLAST of EF-hand calcium-binding domain-containing protein 6 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of EF-hand calcium-binding domain-containing protein 6 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 4
BLAST of EF-hand calcium-binding domain-containing protein 6 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of EF-hand calcium-binding domain-containing protein 6 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 3
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30023059 ID=SMED30023059|Name=EF-hand calcium-binding domain-containing protein 6|organism=Schmidtea mediterranea sexual|type=transcript|length=4864bpback to top protein sequence of SMED30023059-orf-1 >SMED30023059-orf-1 ID=SMED30023059-orf-1|Name=SMED30023059-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=1579bp MTTDITDSLIVRKSIYREPIQSNSLNQPEKNSTIKDANYKMDPKLQISEIback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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